JP5307439B2 - レーザ顕微鏡 - Google Patents
レーザ顕微鏡 Download PDFInfo
- Publication number
- JP5307439B2 JP5307439B2 JP2008105994A JP2008105994A JP5307439B2 JP 5307439 B2 JP5307439 B2 JP 5307439B2 JP 2008105994 A JP2008105994 A JP 2008105994A JP 2008105994 A JP2008105994 A JP 2008105994A JP 5307439 B2 JP5307439 B2 JP 5307439B2
- Authority
- JP
- Japan
- Prior art keywords
- optical fiber
- laser light
- laser
- microscope
- ultrashort pulse
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000013307 optical fiber Substances 0.000 claims description 157
- 239000006185 dispersion Substances 0.000 claims description 109
- 230000003287 optical effect Effects 0.000 claims description 87
- 230000007246 mechanism Effects 0.000 claims description 46
- 238000001514 detection method Methods 0.000 claims description 30
- 239000000835 fiber Substances 0.000 claims description 22
- 230000009022 nonlinear effect Effects 0.000 claims description 21
- 230000008859 change Effects 0.000 claims description 18
- 238000007493 shaping process Methods 0.000 claims description 12
- 230000001902 propagating effect Effects 0.000 claims description 9
- 230000010287 polarization Effects 0.000 claims description 6
- 230000000644 propagated effect Effects 0.000 claims description 6
- 239000004038 photonic crystal Substances 0.000 claims description 4
- 230000000903 blocking effect Effects 0.000 claims 1
- 230000008878 coupling Effects 0.000 description 19
- 238000010168 coupling process Methods 0.000 description 19
- 238000005859 coupling reaction Methods 0.000 description 19
- 230000005284 excitation Effects 0.000 description 13
- 230000005540 biological transmission Effects 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 210000001747 pupil Anatomy 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 238000003384 imaging method Methods 0.000 description 4
- 239000013078 crystal Substances 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- 238000002073 fluorescence micrograph Methods 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- 239000002612 dispersion medium Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000002310 reflectometry Methods 0.000 description 1
- SBIBMFFZSBJNJF-UHFFFAOYSA-N selenium;zinc Chemical compound [Se]=[Zn] SBIBMFFZSBJNJF-UHFFFAOYSA-N 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000003079 width control Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
- Mechanical Light Control Or Optical Switches (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008105994A JP5307439B2 (ja) | 2007-04-23 | 2008-04-15 | レーザ顕微鏡 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007113042 | 2007-04-23 | ||
| JP2007113042 | 2007-04-23 | ||
| JP2008105994A JP5307439B2 (ja) | 2007-04-23 | 2008-04-15 | レーザ顕微鏡 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2008292994A JP2008292994A (ja) | 2008-12-04 |
| JP2008292994A5 JP2008292994A5 (enExample) | 2011-04-07 |
| JP5307439B2 true JP5307439B2 (ja) | 2013-10-02 |
Family
ID=39529618
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008105994A Expired - Fee Related JP5307439B2 (ja) | 2007-04-23 | 2008-04-15 | レーザ顕微鏡 |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US7995271B2 (enExample) |
| EP (1) | EP1986030B1 (enExample) |
| JP (1) | JP5307439B2 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009032916A (ja) * | 2007-07-27 | 2009-02-12 | Fujifilm Corp | 分散補償器およびそれを用いた固体レーザ装置並びに分散補償方法 |
| DE102009048710B4 (de) * | 2009-10-08 | 2020-04-02 | Leica Microsystems Cms Gmbh | Lasersystem für ein Mikroskop und Verfahren zum Betreiben eines Lasersystems für ein Mikroskop |
| EP2312367A1 (en) * | 2009-10-16 | 2011-04-20 | Olympus Corporation | Laser scanning microscope |
| DE102010037190B4 (de) | 2010-08-27 | 2015-11-26 | Leica Microsystems Cms Gmbh | Vorrichtung zum zeitlichen Verschieben von Weißlichtlaserpulsen |
| JP5833299B2 (ja) * | 2010-11-02 | 2015-12-16 | 株式会社ディスコ | レーザー加工装置 |
| DE102011000905A1 (de) | 2011-02-24 | 2012-08-30 | Leica Microsystems Cms Gmbh | Pulsvereiniger für die verschiedenen Spektralfarben eines Superkontinuum-Lasers |
| WO2013089258A1 (ja) * | 2011-12-15 | 2013-06-20 | 株式会社ニコン | 顕微鏡及び刺激装置 |
| WO2014075713A1 (en) * | 2012-11-14 | 2014-05-22 | Wavelight Gmbh | Laser pulse focusing |
| CA2943566C (en) * | 2014-05-23 | 2018-09-04 | Wavelight Gmbh | Measuring module including an interface for coupling to a laser device |
| JP7121606B2 (ja) * | 2018-09-11 | 2022-08-18 | 浜松ホトニクス株式会社 | 光計測装置 |
| CN111122567B (zh) * | 2018-11-01 | 2022-09-16 | 华中科技大学苏州脑空间信息研究院 | 一种高通量光学层析三维成像系统 |
| DE102021103899A1 (de) * | 2021-02-18 | 2022-08-18 | Toptica Photonics Ag | Beleuchtungsvorrichtung für ein Konfokalmikroskop |
| US20220395924A1 (en) * | 2021-06-11 | 2022-12-15 | Electronics And Telecommunications Research Institute | Two-photon microscopy and pulse width correction method using the same |
| CN115685516B (zh) * | 2022-11-01 | 2025-06-20 | 贵州大学 | 一种单色光高光谱系统及控制方法 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1229904A (en) * | 1985-06-28 | 1987-12-01 | John C. Goodwin | Laser-fiber positioner |
| JP2504747B2 (ja) * | 1986-08-08 | 1996-06-05 | 日本電信電話株式会社 | 半導体素子と光伝送路との光軸合せ方法およびその装置 |
| DE19622359B4 (de) * | 1996-06-04 | 2007-11-22 | Carl Zeiss Jena Gmbh | Vorrichtung zur Einkopplung der Strahlung von Kurzpulslasern in einem mikroskopischen Strahlengang |
| US5862287A (en) * | 1996-12-13 | 1999-01-19 | Imra America, Inc. | Apparatus and method for delivery of dispersion compensated ultrashort optical pulses with high peak power |
| JP3014039B2 (ja) * | 1997-01-31 | 2000-02-28 | 株式会社日立製作所 | 光パルス分散補償器、及びこれを用いた光パルス圧縮器、半導体短パルスレーザ素子並びに光通信システム |
| US5995281A (en) * | 1997-04-09 | 1999-11-30 | Carl Zeiss Jena Gmbh | Device for coupling the radiation of short-pulse lasers in an optical beam path of a microscope |
| US5933274A (en) * | 1997-05-15 | 1999-08-03 | Andrew F. DeSimone | Dye laser system |
| JPH10318924A (ja) * | 1997-05-19 | 1998-12-04 | Olympus Optical Co Ltd | パルスレーザを備えた光学装置 |
| US6855941B1 (en) * | 1998-03-11 | 2005-02-15 | Olympus Optical Co., Ltd. | Laser microscope |
| JP2000304619A (ja) * | 1999-04-26 | 2000-11-02 | Oyo Koden Kenkyushitsu:Kk | 群速度分散制御装置および群速度分散測定装置 |
| US6471419B1 (en) * | 1999-06-07 | 2002-10-29 | International Business Machines Corporation | Fiber optic assembly |
| JP4693972B2 (ja) * | 2000-09-29 | 2011-06-01 | オリンパス株式会社 | レーザ顕微鏡 |
| JP4845279B2 (ja) * | 2001-02-09 | 2011-12-28 | オリンパス株式会社 | 生体機能測定方法 |
| JP4854878B2 (ja) * | 2001-07-03 | 2012-01-18 | オリンパス株式会社 | レーザー顕微鏡 |
| JP2003322799A (ja) * | 2002-04-30 | 2003-11-14 | Olympus Optical Co Ltd | レーザ顕微鏡システム |
| DE10332064A1 (de) * | 2003-07-11 | 2005-01-27 | Carl Zeiss Jena Gmbh | Anordnung zur Erfassung der Beleuchtungsstrahlung ineinem Laser-Scanning-Mikroskop |
| US7176428B2 (en) * | 2004-03-12 | 2007-02-13 | Olympus Corporation | Laser-based, multiphoton-excitation-type optical examination apparatus |
| JP4276971B2 (ja) * | 2004-03-12 | 2009-06-10 | オリンパス株式会社 | 多光子励起型測定装置 |
| JP4729269B2 (ja) * | 2004-06-01 | 2011-07-20 | オリンパス株式会社 | レーザ走査型顕微鏡 |
| JP4763979B2 (ja) * | 2004-07-01 | 2011-08-31 | 財団法人光科学技術研究振興財団 | パルス光照射装置 |
| FR2877103B1 (fr) * | 2004-10-22 | 2012-02-10 | Mauna Kea Technologies | Systeme et procede d'imagerie microscopique multiphotonique fibre d'un echantillon |
| JP4878751B2 (ja) * | 2004-12-10 | 2012-02-15 | オリンパス株式会社 | 顕微鏡用照明装置および蛍光顕微鏡装置 |
| JP2006275917A (ja) * | 2005-03-30 | 2006-10-12 | Olympus Corp | 多光子励起型観察装置および多光子励起型観察用光源装置 |
| JP4869734B2 (ja) * | 2005-04-25 | 2012-02-08 | オリンパス株式会社 | 多光子励起走査型レーザ顕微鏡 |
| GB0519761D0 (en) * | 2005-09-28 | 2005-11-09 | Point Source Ltd | Laser systems |
| JP4622800B2 (ja) | 2005-10-19 | 2011-02-02 | 住友金属工業株式会社 | 機械攪拌方式による溶銑の脱燐方法 |
-
2008
- 2008-04-15 JP JP2008105994A patent/JP5307439B2/ja not_active Expired - Fee Related
- 2008-04-17 EP EP08007511.2A patent/EP1986030B1/en not_active Not-in-force
- 2008-04-21 US US12/106,615 patent/US7995271B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US7995271B2 (en) | 2011-08-09 |
| US20080259445A1 (en) | 2008-10-23 |
| JP2008292994A (ja) | 2008-12-04 |
| EP1986030B1 (en) | 2017-08-30 |
| EP1986030A1 (en) | 2008-10-29 |
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