JP5283367B2 - 線維芽細胞増殖因子−1産生促進剤 - Google Patents
線維芽細胞増殖因子−1産生促進剤 Download PDFInfo
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- JP5283367B2 JP5283367B2 JP2007278097A JP2007278097A JP5283367B2 JP 5283367 B2 JP5283367 B2 JP 5283367B2 JP 2007278097 A JP2007278097 A JP 2007278097A JP 2007278097 A JP2007278097 A JP 2007278097A JP 5283367 B2 JP5283367 B2 JP 5283367B2
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Description
イネ科オガルカヤ属植物レモングラスの茎および/または葉を乾燥させて粉砕し、サンプル重量の10倍量の50%1,3−ブチレングリコール水溶液を加えて50℃にて3日間浸漬し、これをろ過し、植物抽出物を得る。
イネ科オガルカヤ属植物レモングラスの茎および/または葉を乾燥させて粉砕し、サンプル5グラムに対して100グラム重量(20倍量)の精製水を加えてオートクレーブ(120度、20min)を使って抽出し、温度の高い状態を保って吸引濾過後、凍結乾燥を行って抽出物を得た。
ヒト表皮角化細胞(HaCaT)を1ウェル当り2.0×104個となるように96ウェルマイクロプレートに播種した。播種培地にはダルベッコ改変イーグル培地(DMEM)に5重量%のウシ胎児血清(FBS)を添加したものを用いた。24時間後、5%重量FBS添加DMEM培地にて各濃度に調整したサンプル(抽出方法1:レモングラス50%1,3−ブチレングリコール抽出物)培養液に交換し、さらに48時間培養した。KGFの産生量の定量にはFGF acidic(FGF−1)ELISA,Human(R&D Systems社製)キットを使用した。キットはサンドイッチELISAの原理を用いている。最後に発色物質を反応させた後、マイクロプレートリーダーにて450nmの吸光度を測定した。評価ではサンプル培養液の他にネガティブコントロールとして5%FBS添加DMEM培地を用いた。PIERCE社製BCA Protein Assay Kitにてタンパク量を測定し単位タンパク量当りのコラーゲン産生量を求めた。評価はネガティブコントロールの単位当りFGF−1産生量を100とした時の相対値を求めて行った。結果を表1(別紙)に示す。なお、表中の*および**は、t検定における有意確率P値に対し、有意確率5%未満(P<0.05)を*、有意確率1%未満(P<0.01)を**で表したものである。
Claims (1)
- イネ科オガルカヤ属レモングラス(Cymbopogon citratus)の水、エタノール、1,3−ブチレングリコール、ジプロピレングリコール、およびグリセリンからなる群から選ばれる1種又は2種以上の溶媒を用いて抽出した抽出物を有効成分とすることを特徴とする表皮角化細胞における線維芽細胞増殖因子−1産生促進剤(化粧料を除く)。
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Families Citing this family (3)
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JP2011051901A (ja) * | 2009-08-31 | 2011-03-17 | Riyo Miura | α−グルコシダーゼ阻害剤 |
JP6324458B2 (ja) * | 2016-09-21 | 2018-05-16 | 一丸ファルコス株式会社 | キネシン抑制剤 |
KR102229902B1 (ko) * | 2019-07-30 | 2021-03-19 | 재단법인 남원시화장품산업지원센터 | 페놀성화합물을 유효성분으로 하는 혼합 추출물을 포함하는 피부 보습 및 피부 장벽 개선 화장료 조성물 |
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JP4056570B2 (ja) * | 1994-03-31 | 2008-03-05 | 一丸ファルコス株式会社 | レモングラスの抽出物含有化粧料 |
JP2003321385A (ja) * | 2002-05-09 | 2003-11-11 | Kanegafuchi Chem Ind Co Ltd | Tnf産生促進剤 |
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