JP5231271B2 - 側面通気用の蓋を含む培養プレート - Google Patents
側面通気用の蓋を含む培養プレート Download PDFInfo
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- JP5231271B2 JP5231271B2 JP2009022005A JP2009022005A JP5231271B2 JP 5231271 B2 JP5231271 B2 JP 5231271B2 JP 2009022005 A JP2009022005 A JP 2009022005A JP 2009022005 A JP2009022005 A JP 2009022005A JP 5231271 B2 JP5231271 B2 JP 5231271B2
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- lid
- filter element
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- culture
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- 238000009423 ventilation Methods 0.000 title abstract description 3
- 239000000463 material Substances 0.000 claims abstract description 28
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- 238000004519 manufacturing process Methods 0.000 claims description 10
- 239000004698 Polyethylene Substances 0.000 claims description 9
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- 229920000573 polyethylene Polymers 0.000 claims description 9
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- 230000000717 retained effect Effects 0.000 claims description 4
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- 238000005245 sintering Methods 0.000 claims description 3
- 238000003466 welding Methods 0.000 claims description 2
- 230000008020 evaporation Effects 0.000 abstract description 2
- 238000001704 evaporation Methods 0.000 abstract description 2
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- 238000007789 sealing Methods 0.000 description 12
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- 241000233866 Fungi Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000001746 injection moulding Methods 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 238000003856 thermoforming Methods 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
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- 239000004793 Polystyrene Substances 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
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- 238000005273 aeration Methods 0.000 description 1
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- 238000010438 heat treatment Methods 0.000 description 1
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- 238000009630 liquid culture Methods 0.000 description 1
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- 238000013022 venting Methods 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
Claims (14)
- a)底部側、少なくとも1つの側壁、及び上部側を含む基板、及び該基板に所定のパターンで配置され、互いに分離している複数の空洞、並びに各空洞を取り囲む少なくとも1つの側壁を含む底部要素;底部壁;並びに物質(material)を該空洞に保持されるように受け入れ、及び該基板の上部側から接近可能とするための開口端;
b)上部及び周囲側壁を含む前記底部要素を覆うための蓋であって、少なくとも該蓋の周辺側壁が、フィルター材料で作られている少なくとも1つのフィルター要素から少なくとも部分的に形成されていることによって特徴付けられる蓋であって、ここで、培養プレートの内部体積と周辺環境との間のガス交換が該少なくとも1つのフィルター要素を介して達成される前記蓋
を含む培養プレート。 - 前記基板の底部側が、該基板の少なくとも1つの側壁の上方に伸びて、蓋の少なくとも1つの側壁の底部側が培養プレートの密着した配置で支えられている伸長を提供する、請求項1に記載の培養プレート。
- 前記少なくとも1つのフィルター要素が、機械的に安定な要素である、請求項1又は2に記載の培養プレート。
- 前記蓋の少なくとも1つの側壁がフィルター要素で完全に存在している、請求項1〜3のいずれか1項に記載の培養プレート。
- さらに、前記蓋の上部は、少なくとも1つの作製されたフィルター要素を含み、完全にフィルター要素からなる、請求項1〜4のいずれか1項に記載の培養プレート。
- 前記フィルター要素が、0.1〜1μmの範囲の孔サイズを有する、請求項1〜5のいずれか1項に記載の培養プレート。
- 前記フィルター要素が焼結されたポリエチレンから作られている、請求項1〜6のいずれか1項に記載の培養プレート。
- 前記空洞が、行及び列で配置されている、請求項1に記載の培養プレート。
- 前記底部要素が、従来のマイクロタイタープレートに基づいた寸法及びレイアウトを有する、請求項8に記載の培養プレート。
- 前記マイクロタイタープレートが、プレート当たり4、6、8、12、24、48、96、384又は1536個の空洞を含む、請求項9に記載の培養プレート。
- 請求項1〜10のいずれか1項に記載の培養プレートを製造する方法であって、
a)所定のパターンで配置され、互いに分離している複数の空洞を有する底部要素を用意し、
b)フィルター材料で作られている少なくとも1つのフィルター要素を用意し、
c)上部のみ又は上部から伸びている少なくとも1つの周囲の側壁を有する上部からなり、少なくとも1つのフィルター要素を受け入れるように適合された少なくとも1つの凹所を有する蓋を提供し、そして
d)該少なくとも1つのフィルター要素と該蓋とを連結する
工程を含む前記方法。 - 工程b)において、蓋の完全な側壁を形成するフィルター要素が提供される、請求項11に記載の方法。
- 前記フィルター要素の提供が、ポリエチレン粉末を鋳型に充填し、それを焼結過程に供することによる該フィルター要素の製造を含む、請求項12に記載の方法。
- 前記フィルター要素が、留め(snapping)、接着を伴う固着、又は超音波溶着によって前記蓋に連結される、請求項11〜13のいずれか1項に記載の方法。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP08001920.1 | 2008-02-01 | ||
EP08001920A EP2085463B1 (en) | 2008-02-01 | 2008-02-01 | Culture plate comprising a lid for lateral ventilation |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2009183288A JP2009183288A (ja) | 2009-08-20 |
JP5231271B2 true JP5231271B2 (ja) | 2013-07-10 |
Family
ID=39598439
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009022005A Active JP5231271B2 (ja) | 2008-02-01 | 2009-02-02 | 側面通気用の蓋を含む培養プレート |
Country Status (5)
Country | Link |
---|---|
US (1) | US8658422B2 (ja) |
EP (1) | EP2085463B1 (ja) |
JP (1) | JP5231271B2 (ja) |
AT (1) | ATE481472T1 (ja) |
DE (1) | DE602008002545D1 (ja) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9790465B2 (en) | 2013-04-30 | 2017-10-17 | Corning Incorporated | Spheroid cell culture well article and methods thereof |
CN103814767A (zh) * | 2014-02-07 | 2014-05-28 | 兰州大学 | 苔藓植物实验材料快速培养装置 |
JP6930914B2 (ja) | 2014-10-29 | 2021-09-01 | コーニング インコーポレイテッド | 灌流バイオリアクタ・プラットフォーム |
SG11201703493SA (en) | 2014-10-29 | 2017-05-30 | Corning Inc | Cell culture insert |
JP2019030222A (ja) * | 2015-12-25 | 2019-02-28 | 東京エレクトロン株式会社 | 培養容器 |
US11857970B2 (en) | 2017-07-14 | 2024-01-02 | Corning Incorporated | Cell culture vessel |
JP7245222B2 (ja) | 2017-07-14 | 2023-03-23 | コーニング インコーポレイテッド | 手動又は自動で培地を交換するための3d細胞培養容器 |
WO2019014610A1 (en) | 2017-07-14 | 2019-01-17 | Corning Incorporated | CELL CULTURE CONTAINER FOR 3D CULTURE AND METHODS OF CULTURING 3D CELLS |
CN107287095A (zh) * | 2017-08-24 | 2017-10-24 | 熹农生物科技(涟源)有限公司 | 微生物培养容器 |
JP7171695B2 (ja) * | 2018-07-13 | 2022-11-15 | コーニング インコーポレイテッド | 液体培地送達面を含む側壁を有するマイクロキャビティ皿 |
WO2020013845A1 (en) | 2018-07-13 | 2020-01-16 | Corning Incorporated | Cell culture vessels with stabilizer devices |
CN111065725B (zh) | 2018-07-13 | 2024-03-29 | 康宁股份有限公司 | 包括具有互联的壁的微板的流体装置 |
EP4363550A1 (en) * | 2021-06-30 | 2024-05-08 | Corning Incorporated | Protective carrier for microcavity vessels |
Family Cites Families (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4299921A (en) * | 1979-03-30 | 1981-11-10 | Youssef Kamal A | Prolonged incubation microbiological apparatus and filter gaskets thereof |
US4786601A (en) * | 1985-03-15 | 1988-11-22 | Rothenberg Barry E | Tissue culture holder |
US4670398A (en) | 1986-02-24 | 1987-06-02 | Song John S | Plant tissue culture vessel and filter |
US4754840B1 (en) * | 1987-10-07 | 1997-01-14 | Jlg Ind Inc | Leveling assembly for work platforms on articulating booms |
JPH0198600U (ja) * | 1987-12-21 | 1989-06-30 | ||
US5554533A (en) * | 1990-04-11 | 1996-09-10 | Commonwealth Scientific And Industrial Research Organisation | Apparatus and method for rearing nematodes, fungi, tissue cultures and the like, and for harvesting nematodes |
US5520302A (en) * | 1994-01-27 | 1996-05-28 | Anderson; Roger | Petri dish having two-position lid |
WO1996029856A1 (en) | 1995-03-28 | 1996-10-03 | Osmotek Ltd. | Heat sealed container and method of use in plant culture |
JPH09235399A (ja) * | 1996-02-27 | 1997-09-09 | Nitto Denko Corp | 多孔質フィルム |
US5817509A (en) * | 1997-03-19 | 1998-10-06 | Becton Dickinson And Company | Culture vessel assembly |
US5780294A (en) * | 1997-03-19 | 1998-07-14 | Becton Dickinson And Company | Culture vessel assembly |
US5863792A (en) | 1997-03-19 | 1999-01-26 | Becton Dickson And Company | Culture vessel assembly |
JP3761676B2 (ja) * | 1997-07-11 | 2006-03-29 | 株式会社メニコン | 細胞培養用容器 |
EP1069181A3 (en) | 1999-07-13 | 2002-03-27 | Whatman, Inc. | Closure assembly for multiwell vessel |
FR2803852B1 (fr) * | 2000-01-17 | 2004-11-05 | Farzin Sarem | Dispositif de culture cellulaire et tissulaire a circulation de fluide de culture controlee |
US20020012982A1 (en) * | 2000-07-13 | 2002-01-31 | Invitrogen Corporation | Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix |
DE10244859B4 (de) * | 2002-09-20 | 2007-01-11 | Leibniz-Institut Für Polymerforschung Dresden E.V. | Bioreaktor mit modularem Aufbau, insbesondere zur ex-vivo Zellvermehrung |
JP4101638B2 (ja) * | 2002-12-24 | 2008-06-18 | 日鉄鉱業株式会社 | フィルタエレメント及びその製造方法 |
US20050074873A1 (en) * | 2003-09-09 | 2005-04-07 | Shanler Michael S. | Tissue culture vessel |
US20050178054A1 (en) | 2003-12-09 | 2005-08-18 | Hsu Wei K. | Stacking autoclaveable mushroom culture jar with filter lid |
US7323024B2 (en) * | 2003-12-10 | 2008-01-29 | Babcock & Wilcox Technical Services Y-12, Llc | Vessel with filter and method of use |
DE102004024834A1 (de) * | 2004-05-19 | 2006-01-12 | Universität Rostock | Vorrichtung zur Durchführung einer Liquid-Air-Kultur von Epithel |
JP2006204263A (ja) * | 2005-01-31 | 2006-08-10 | Olympus Corp | 培養容器および培養方法 |
DE102006004157A1 (de) * | 2006-01-30 | 2007-08-02 | Eppendorf Ag | Vorrichtung und Verfahren zum Inkubieren von Zellen |
US20080003670A1 (en) * | 2006-06-30 | 2008-01-03 | Corning Incorporated | High density permeable supports for high throughput screening |
-
2008
- 2008-02-01 AT AT08001920T patent/ATE481472T1/de not_active IP Right Cessation
- 2008-02-01 DE DE602008002545T patent/DE602008002545D1/de active Active
- 2008-02-01 EP EP08001920A patent/EP2085463B1/en active Active
-
2009
- 2009-02-02 JP JP2009022005A patent/JP5231271B2/ja active Active
- 2009-02-02 US US12/364,371 patent/US8658422B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
EP2085463A1 (en) | 2009-08-05 |
EP2085463B1 (en) | 2010-09-15 |
ATE481472T1 (de) | 2010-10-15 |
US20090253197A1 (en) | 2009-10-08 |
DE602008002545D1 (de) | 2010-10-28 |
JP2009183288A (ja) | 2009-08-20 |
US8658422B2 (en) | 2014-02-25 |
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