JP5192229B2 - microRNAおよび低分子干渉RNAの定量化のための新規方法 - Google Patents

microRNAおよび低分子干渉RNAの定量化のための新規方法 Download PDF

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JP5192229B2
JP5192229B2 JP2007506659A JP2007506659A JP5192229B2 JP 5192229 B2 JP5192229 B2 JP 5192229B2 JP 2007506659 A JP2007506659 A JP 2007506659A JP 2007506659 A JP2007506659 A JP 2007506659A JP 5192229 B2 JP5192229 B2 JP 5192229B2
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sequence
rna
probe
nucleotide
lna
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JP2007532100A (ja
JP2007532100A5 (https=
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ナナ・ヤコブセン
ラース・コングスバク
サカリ・カウッピネン
セレン・モーゲンタラー・エヒヴァルズ
ペーター・モウリツェン
ペーター・ステイン・ニールセン
ミッケル・ネアホルム
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates

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JP2007506659A 2004-04-07 2005-04-07 microRNAおよび低分子干渉RNAの定量化のための新規方法 Expired - Lifetime JP5192229B2 (ja)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
DKPA200400578 2004-04-07
DKPA200400578 2004-04-07
DKPA200401146 2004-07-23
DKPA200401146 2004-07-23
DKPA200401218 2004-08-11
DKPA200401218 2004-08-11
DKPA200401587 2004-10-15
DKPA200401587 2004-10-15
DKPA200500140 2005-01-28
DKPA200500140 2005-01-28
PCT/DK2005/000239 WO2005098029A2 (en) 2004-04-07 2005-04-07 Methods for quantification of micrornas and small interfering rnas

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JP2007532100A JP2007532100A (ja) 2007-11-15
JP2007532100A5 JP2007532100A5 (https=) 2012-06-21
JP5192229B2 true JP5192229B2 (ja) 2013-05-08

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JP (1) JP5192229B2 (https=)
CN (1) CN1961081B (https=)
AT (1) ATE542918T1 (https=)
CA (1) CA2562390C (https=)
DK (1) DK1735459T3 (https=)
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10525471B2 (en) 2015-01-22 2020-01-07 Arkray, Inc. Target analysis chip and target analysis method

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040219565A1 (en) 2002-10-21 2004-11-04 Sakari Kauppinen Oligonucleotides useful for detecting and analyzing nucleic acids of interest
US8192937B2 (en) 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
EP1838870A2 (en) * 2004-12-29 2007-10-03 Exiqon A/S NOVEL OLIGONUCLEOTIDE COMPOSITIONS AND PROBE SEQUENCES USEFUL FOR DETECTION AND ANALYSIS OF MICRORNAS AND THEIR TARGET MRNAs
JP2008528003A (ja) * 2005-01-25 2008-07-31 ロゼッタ インファーマティックス エルエルシー 小rna分子の定量方法
US8071306B2 (en) 2005-01-25 2011-12-06 Merck Sharp & Dohme Corp. Methods for quantitating small RNA molecules
US7556943B2 (en) * 2005-05-31 2009-07-07 Applied Biosystems, Llc Linear amplification of short nucleic acids
JP2007082436A (ja) * 2005-09-20 2007-04-05 Bioinformatics Institute For Global Good Inc 機能性RNAが制御するターゲットmRNAの予測・同定方法及びその利用方法
WO2007073737A1 (en) * 2005-12-29 2007-07-05 Exiqon A/S Detection of tissue origin of cancer
SG10201406016SA (en) 2006-04-03 2014-11-27 Stella Aps Pharmaceutical composition comprising anti-mirna antisense oligonucleotides
US7955848B2 (en) 2006-04-03 2011-06-07 Trustees Of Dartmouth College MicroRNA biomarkers for human breast and lung cancer
EP2007888A2 (en) 2006-04-03 2008-12-31 Santaris Pharma A/S Pharmaceutical composition comprising anti-mirna antisense oligonucleotides
US8207325B2 (en) 2006-04-03 2012-06-26 Univ. of Copenhagen MicroRNA biomarkers for human breast and lung cancer
US8580494B2 (en) 2006-08-25 2013-11-12 Research Foundation For Mental Hygiene, Inc. Methods and compositions for amplification and detection of MicroRNAs
WO2008040355A2 (en) * 2006-10-06 2008-04-10 Exiqon A/S Novel methods for quantification of micrornas and small interfering rnas
US8188255B2 (en) 2006-10-20 2012-05-29 Exiqon A/S Human microRNAs associated with cancer
EP2090665A2 (en) 2006-10-20 2009-08-19 Exiqon A/S Novel human microRNAs associated with cancer
EP1914317A1 (en) * 2006-10-21 2008-04-23 Biolytix AG A method for qualitative and quantitative detection of short nucleic acid sequences of about 8 to 50 nucleotides in length
US7645578B2 (en) 2006-10-31 2010-01-12 Agilent Technologies, Inc. Cleavage of RNA at redundant sites
US8470791B2 (en) 2007-03-22 2013-06-25 Santaris Pharma A/S RNA antagonist compounds for the inhibition of Apo-B100 expression
DK2149605T3 (da) 2007-03-22 2013-09-30 Santaris Pharma As Korte RNA antagonist forbindelser til modulering af det ønskede mRNA
EP1978104A1 (en) * 2007-04-03 2008-10-08 Freie Universität Berlin A method for the quantitative analysis of RNA molecules in a sample
EP2623599B1 (en) 2007-10-04 2019-01-02 Roche Innovation Center Copenhagen A/S Micromirs
EP2198059B1 (en) * 2007-10-09 2014-12-03 Igor Vassily Kutyavin Methods for the improved detection of small rna molecules
KR20100099158A (ko) 2007-11-09 2010-09-10 보드 오브 리전츠 더 유니버시티 오브 텍사스 시스템 심근세포 생존과 심장 회복을 제어하는 MIR-15 집단의 마이크로RNAs
WO2009098988A1 (ja) 2008-02-04 2009-08-13 Genecare Research Institute Co., Ltd. siRNA検出方法
CA2717792A1 (en) 2008-03-07 2009-09-11 Santaris Pharma A/S Pharmaceutical compositions for treatment of microrna related diseases
WO2010012667A1 (en) 2008-08-01 2010-02-04 Santaris Pharma A/S Micro-rna mediated modulation of colony stimulating factors
US20100240049A1 (en) 2009-01-16 2010-09-23 Cepheid Methods of Detecting Cervical Cancer
CA2787218A1 (en) * 2009-01-19 2010-07-22 Sistemic Scotland Limited Methods employing non-coding rna expression assays
DK2391736T3 (en) * 2009-02-02 2015-05-11 Exiqon As A method for the quantitation of small RNA species
US9493832B2 (en) 2009-02-02 2016-11-15 Cepheid Methods of detecting sepsis
EP2419537B1 (en) 2009-04-13 2014-01-29 Somagenics, Inc. Methods and compositions for detection of small rnas
ES2599979T3 (es) 2009-04-24 2017-02-06 Roche Innovation Center Copenhagen A/S Composiciones farmacéuticas para el tratamiento de pacientes de VHC que no responden al interferón
EP2456870A1 (en) 2009-07-21 2012-05-30 Santaris Pharma A/S Antisense oligomers targeting pcsk9
KR101822609B1 (ko) * 2010-06-24 2018-01-26 주식회사 파나진 Pna 기반의 실시간 pcr 클램핑을 이용한 마이크로rna 발현양상의 분석 방법 및 키트
US9200314B2 (en) * 2010-07-21 2015-12-01 Takara Bio Inc. Synthetic siRNA detection method
CA2825522A1 (en) 2011-01-26 2012-08-02 Cepheid Methods of detecting lung cancer
CN102154505B (zh) * 2011-04-20 2013-03-20 苟德明 一种检测miRNA的方法及引物及其应用
EP2794926B1 (en) 2011-12-22 2018-01-17 SomaGenics Inc. Methods of constructing small rna libraries and their use for expression profiling of target rnas
JP2015507928A (ja) * 2012-02-14 2015-03-16 ザ・ジョンズ・ホプキンス・ユニバーシティ Mirna解析方法
US9163235B2 (en) 2012-06-21 2015-10-20 MiRagen Therapeutics, Inc. Inhibitors of the miR-15 family of micro-RNAs
DK2914741T3 (da) 2012-11-02 2017-11-20 Life Technologies Corp Hidtil ukendte sammensætninger og fremgangsmåder til forbedring af PCR-specificitet
US9862987B2 (en) 2013-01-16 2018-01-09 The Regents Of The University Of California Label free molecular detection methods, systems and devices
WO2014113598A2 (en) 2013-01-16 2014-07-24 The Regents Of The University Of California Microfluidic devices to extract, concentrate and isolate molecules
EP2971144B1 (en) * 2013-03-14 2019-10-30 Aegea Biotechnologies, Inc. Method for amplification of nucleic acids on solid support
CN103218543B (zh) * 2013-03-27 2016-04-13 中国科学院计算技术研究所 一种区分蛋白编码基因和非编码基因的方法及系统
HUE048738T2 (hu) 2013-06-27 2020-08-28 Roche Innovation Ct Copenhagen As Antiszensz oligomerek és konjugátumok, amelyek a PCK9-t célozzák meg
CN104109708B (zh) * 2013-12-31 2015-05-13 厦门成坤生物技术有限公司 一种直接测定生物样品中未经分离的小核酸的方法及检测试剂盒
EP2942400A1 (en) 2014-05-09 2015-11-11 Lifecodexx AG Multiplex detection of DNA that originates from a specific cell-type
JP6681841B2 (ja) * 2014-05-09 2020-04-15 ライフコーデックス アーゲー 特別な細胞タイプに由来するdnaの検出とそれに関連する方法
PT3204520T (pt) * 2014-10-08 2020-04-16 Univ Cornell Método de identificação e quantificação da expressão de ácidos nucleicos, variantes de splicing, translocação, número de cópias ou alterações de metilação
CN104598775B (zh) * 2014-12-31 2018-10-16 北京邮电大学 一种rna编辑事件识别方法
CN104673901B (zh) * 2015-02-06 2018-05-11 中国医学科学院基础医学研究所 用于检测靶核酸的用途和试剂盒
EP3967768A1 (en) 2015-03-13 2022-03-16 Life Technologies Corporation Compositions for small rna capture, detection and quantification
CN104975078A (zh) * 2015-05-18 2015-10-14 上海市农业科学院 一种检测转基因植物中靶标siRNA池的方法
TWI676679B (zh) * 2015-06-02 2019-11-11 奎克生技光電股份有限公司 核苷酸序列、通用反向引子、通用反轉錄引子、引子設計方法及miRNA檢測方法
HUE050491T2 (hu) 2015-11-10 2020-12-28 Eurofins Lifecodexx Gmbh Magzati kromoszomális aneuploidiák kimutatása olyan DNS régiókat alkalmazva, amelyek különbözõképpen vannak metilezve a magzat és a terhes nõstény között
WO2017112666A1 (en) 2015-12-21 2017-06-29 Somagenics, Inc. Methods of library construction for polynucleotide sequencing
JP6577660B2 (ja) 2016-03-18 2019-09-18 株式会社東芝 試料中の複数種類の短鎖核酸の検出方法、組み合わせ分析キットおよび分析キット供給管理方法
CZ2016248A3 (cs) * 2016-04-29 2017-12-20 Ústav molekulární genetiky AV ČR, v.v.i. Způsob detekce specifických mikroRNA pomocí templátové DNA a molekula templátové DNA pro použití při tomto způsobu
WO2019011875A1 (en) * 2017-07-11 2019-01-17 Qiagen Gmbh QUANTIFICATION OF ANTISENSE OLIGONUCLEOTIDES (ASOS)
US20220298555A1 (en) * 2018-12-20 2022-09-22 Roche Molecular Systems, Inc. Detection of target nucleic acid by solid-phase molography
WO2020239822A1 (en) * 2019-05-27 2020-12-03 The European Molecular Biology Laboratory Nucleic acid construct binding to influenza polymerase pb1 rna synthesis active site
EP3901286A1 (en) * 2020-04-24 2021-10-27 Mirnax Biosens, S.L. Bivalent reverse primer
ES3030914T3 (en) * 2019-11-04 2025-07-02 Mirnax Biosens S L Bivalent reverse primer
CN111665351B (zh) * 2020-06-20 2021-12-03 江南大学 一种快速、特异性测定rna含量的方法
CN113322523B (zh) * 2021-06-17 2024-03-19 翌圣生物科技(上海)股份有限公司 Rna快速建库方法及其应用
CN116200492B (zh) * 2022-11-08 2025-04-01 浙江大学 一种基于探针锚定双链的小核酸检测试剂盒及其应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6695600A (en) * 1999-07-19 2001-02-05 Cambridge University Technical Services Limited A method for amplifying low abundance nucleic acid sequences and means for performing said method
WO2002057479A2 (en) * 2000-12-27 2002-07-25 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US20040235005A1 (en) * 2002-10-23 2004-11-25 Ernest Friedlander Methods and composition for detecting targets
US7217807B2 (en) * 2002-11-26 2007-05-15 Rosetta Genomics Ltd Bioinformatically detectable group of novel HIV regulatory genes and uses thereof
JP4230457B2 (ja) * 2002-12-18 2009-02-25 サード・ウェーブ・テクノロジーズ・インク 低分子核酸の検出方法
EP1644533A4 (en) * 2003-07-02 2007-11-14 Perkinelmer Las Inc ANALYSIS AND METHOD OF MARKING AND DETECTING RNA MICROSEQUENCES AND SMALL INTERFERENCE RNA SEQUENCES
JP2007512805A (ja) * 2003-10-14 2007-05-24 ノバルティス アクチエンゲゼルシャフト オリゴヌクレオチドマイクロアレイ

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10525471B2 (en) 2015-01-22 2020-01-07 Arkray, Inc. Target analysis chip and target analysis method

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CN1961081B (zh) 2012-09-05
ES2380923T3 (es) 2012-05-21
WO2005098029A3 (en) 2005-12-15
EP1735459A2 (en) 2006-12-27
CA2562390A1 (en) 2005-10-20
JP2007532100A (ja) 2007-11-15
DK1735459T3 (da) 2012-05-29
CN1961081A (zh) 2007-05-09
CA2562390C (en) 2014-12-02
EP1735459B1 (en) 2012-01-25
WO2005098029A2 (en) 2005-10-20
ATE542918T1 (de) 2012-02-15

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