DK1735459T3 - Fremgangsmåder til kvantificering af microRNA'er og små interfererende RNA'er - Google Patents

Fremgangsmåder til kvantificering af microRNA'er og små interfererende RNA'er

Info

Publication number
DK1735459T3
DK1735459T3 DK05715157.3T DK05715157T DK1735459T3 DK 1735459 T3 DK1735459 T3 DK 1735459T3 DK 05715157 T DK05715157 T DK 05715157T DK 1735459 T3 DK1735459 T3 DK 1735459T3
Authority
DK
Denmark
Prior art keywords
methods
quantification
expression
monitoring
well
Prior art date
Application number
DK05715157.3T
Other languages
Danish (da)
English (en)
Inventor
Nana Jacobsen
Lars Kongsbak
Sakari Kauppinen
Peter Mouritzen
Soeren Morgenthaler Echwald
Peter Stein Nielsen
Mikkel Noerholm
Original Assignee
Exiqon As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exiqon As filed Critical Exiqon As
Application granted granted Critical
Publication of DK1735459T3 publication Critical patent/DK1735459T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DK05715157.3T 2004-04-07 2005-04-07 Fremgangsmåder til kvantificering af microRNA'er og små interfererende RNA'er DK1735459T3 (da)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DKPA200400578 2004-04-07
DKPA200401146 2004-07-23
DKPA200401218 2004-08-11
DKPA200401587 2004-10-15
DKPA200500140 2005-01-28
PCT/DK2005/000239 WO2005098029A2 (en) 2004-04-07 2005-04-07 Methods for quantification of micrornas and small interfering rnas

Publications (1)

Publication Number Publication Date
DK1735459T3 true DK1735459T3 (da) 2012-05-29

Family

ID=35116021

Family Applications (1)

Application Number Title Priority Date Filing Date
DK05715157.3T DK1735459T3 (da) 2004-04-07 2005-04-07 Fremgangsmåder til kvantificering af microRNA'er og små interfererende RNA'er

Country Status (8)

Country Link
EP (1) EP1735459B1 (https=)
JP (1) JP5192229B2 (https=)
CN (1) CN1961081B (https=)
AT (1) ATE542918T1 (https=)
CA (1) CA2562390C (https=)
DK (1) DK1735459T3 (https=)
ES (1) ES2380923T3 (https=)
WO (1) WO2005098029A2 (https=)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040219565A1 (en) 2002-10-21 2004-11-04 Sakari Kauppinen Oligonucleotides useful for detecting and analyzing nucleic acids of interest
US8192937B2 (en) 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
EP1838870A2 (en) * 2004-12-29 2007-10-03 Exiqon A/S NOVEL OLIGONUCLEOTIDE COMPOSITIONS AND PROBE SEQUENCES USEFUL FOR DETECTION AND ANALYSIS OF MICRORNAS AND THEIR TARGET MRNAs
JP2008528003A (ja) * 2005-01-25 2008-07-31 ロゼッタ インファーマティックス エルエルシー 小ｒｎａ分子の定量方法
US8071306B2 (en) 2005-01-25 2011-12-06 Merck Sharp & Dohme Corp. Methods for quantitating small RNA molecules
US7556943B2 (en) * 2005-05-31 2009-07-07 Applied Biosystems, Llc Linear amplification of short nucleic acids
JP2007082436A (ja) * 2005-09-20 2007-04-05 Bioinformatics Institute For Global Good Inc 機能性ＲＮＡが制御するターゲットｍＲＮＡの予測・同定方法及びその利用方法
WO2007073737A1 (en) * 2005-12-29 2007-07-05 Exiqon A/S Detection of tissue origin of cancer
SG10201406016SA (en) 2006-04-03 2014-11-27 Stella Aps Pharmaceutical composition comprising anti-mirna antisense oligonucleotides
US7955848B2 (en) 2006-04-03 2011-06-07 Trustees Of Dartmouth College MicroRNA biomarkers for human breast and lung cancer
EP2007888A2 (en) 2006-04-03 2008-12-31 Santaris Pharma A/S Pharmaceutical composition comprising anti-mirna antisense oligonucleotides
US8207325B2 (en) 2006-04-03 2012-06-26 Univ. of Copenhagen MicroRNA biomarkers for human breast and lung cancer
US8580494B2 (en) 2006-08-25 2013-11-12 Research Foundation For Mental Hygiene, Inc. Methods and compositions for amplification and detection of MicroRNAs
WO2008040355A2 (en) * 2006-10-06 2008-04-10 Exiqon A/S Novel methods for quantification of micrornas and small interfering rnas
US8188255B2 (en) 2006-10-20 2012-05-29 Exiqon A/S Human microRNAs associated with cancer
EP2090665A2 (en) 2006-10-20 2009-08-19 Exiqon A/S Novel human microRNAs associated with cancer
EP1914317A1 (en) * 2006-10-21 2008-04-23 Biolytix AG A method for qualitative and quantitative detection of short nucleic acid sequences of about 8 to 50 nucleotides in length
US7645578B2 (en) 2006-10-31 2010-01-12 Agilent Technologies, Inc. Cleavage of RNA at redundant sites
US8470791B2 (en) 2007-03-22 2013-06-25 Santaris Pharma A/S RNA antagonist compounds for the inhibition of Apo-B100 expression
DK2149605T3 (da) 2007-03-22 2013-09-30 Santaris Pharma As Korte RNA antagonist forbindelser til modulering af det ønskede mRNA
EP1978104A1 (en) * 2007-04-03 2008-10-08 Freie Universität Berlin A method for the quantitative analysis of RNA molecules in a sample
EP2623599B1 (en) 2007-10-04 2019-01-02 Roche Innovation Center Copenhagen A/S Micromirs
EP2198059B1 (en) * 2007-10-09 2014-12-03 Igor Vassily Kutyavin Methods for the improved detection of small rna molecules
KR20100099158A (ko) 2007-11-09 2010-09-10 보드 오브 리전츠 더 유니버시티 오브 텍사스 시스템 심근세포 생존과 심장 회복을 제어하는 ＭＩＲ-15 집단의 마이크로ＲＮＡｓ
WO2009098988A1 (ja) 2008-02-04 2009-08-13 Genecare Research Institute Co., Ltd. siRNA検出方法
CA2717792A1 (en) 2008-03-07 2009-09-11 Santaris Pharma A/S Pharmaceutical compositions for treatment of microrna related diseases
WO2010012667A1 (en) 2008-08-01 2010-02-04 Santaris Pharma A/S Micro-rna mediated modulation of colony stimulating factors
US20100240049A1 (en) 2009-01-16 2010-09-23 Cepheid Methods of Detecting Cervical Cancer
CA2787218A1 (en) * 2009-01-19 2010-07-22 Sistemic Scotland Limited Methods employing non-coding rna expression assays
DK2391736T3 (en) * 2009-02-02 2015-05-11 Exiqon As A method for the quantitation of small RNA species
US9493832B2 (en) 2009-02-02 2016-11-15 Cepheid Methods of detecting sepsis
EP2419537B1 (en) 2009-04-13 2014-01-29 Somagenics, Inc. Methods and compositions for detection of small rnas
ES2599979T3 (es) 2009-04-24 2017-02-06 Roche Innovation Center Copenhagen A/S Composiciones farmacéuticas para el tratamiento de pacientes de VHC que no responden al interferón
EP2456870A1 (en) 2009-07-21 2012-05-30 Santaris Pharma A/S Antisense oligomers targeting pcsk9
KR101822609B1 (ko) * 2010-06-24 2018-01-26 주식회사 파나진 Ｐｎａ 기반의 실시간 ｐｃｒ 클램핑을 이용한 마이크로ｒｎａ 발현양상의 분석 방법 및 키트
US9200314B2 (en) * 2010-07-21 2015-12-01 Takara Bio Inc. Synthetic siRNA detection method
CA2825522A1 (en) 2011-01-26 2012-08-02 Cepheid Methods of detecting lung cancer
CN102154505B (zh) * 2011-04-20 2013-03-20 苟德明 一种检测miRNA的方法及引物及其应用
EP2794926B1 (en) 2011-12-22 2018-01-17 SomaGenics Inc. Methods of constructing small rna libraries and their use for expression profiling of target rnas
JP2015507928A (ja) * 2012-02-14 2015-03-16 ザ・ジョンズ・ホプキンス・ユニバーシティ Ｍｉｒｎａ解析方法
US9163235B2 (en) 2012-06-21 2015-10-20 MiRagen Therapeutics, Inc. Inhibitors of the miR-15 family of micro-RNAs
DK2914741T3 (da) 2012-11-02 2017-11-20 Life Technologies Corp Hidtil ukendte sammensætninger og fremgangsmåder til forbedring af PCR-specificitet
US9862987B2 (en) 2013-01-16 2018-01-09 The Regents Of The University Of California Label free molecular detection methods, systems and devices
WO2014113598A2 (en) 2013-01-16 2014-07-24 The Regents Of The University Of California Microfluidic devices to extract, concentrate and isolate molecules
EP2971144B1 (en) * 2013-03-14 2019-10-30 Aegea Biotechnologies, Inc. Method for amplification of nucleic acids on solid support
CN103218543B (zh) * 2013-03-27 2016-04-13 中国科学院计算技术研究所 一种区分蛋白编码基因和非编码基因的方法及系统
HUE048738T2 (hu) 2013-06-27 2020-08-28 Roche Innovation Ct Copenhagen As Antiszensz oligomerek és konjugátumok, amelyek a PCK9-t célozzák meg
CN104109708B (zh) * 2013-12-31 2015-05-13 厦门成坤生物技术有限公司 一种直接测定生物样品中未经分离的小核酸的方法及检测试剂盒
EP2942400A1 (en) 2014-05-09 2015-11-11 Lifecodexx AG Multiplex detection of DNA that originates from a specific cell-type
JP6681841B2 (ja) * 2014-05-09 2020-04-15 ライフコーデックス アーゲー 特別な細胞タイプに由来するｄｎａの検出とそれに関連する方法
PT3204520T (pt) * 2014-10-08 2020-04-16 Univ Cornell Método de identificação e quantificação da expressão de ácidos nucleicos, variantes de splicing, translocação, número de cópias ou alterações de metilação
CN104598775B (zh) * 2014-12-31 2018-10-16 北京邮电大学 一种rna编辑事件识别方法
WO2016117700A1 (ja) 2015-01-22 2016-07-28 アークレイ株式会社 ターゲット分析チップおよびターゲット分析方法
CN104673901B (zh) * 2015-02-06 2018-05-11 中国医学科学院基础医学研究所 用于检测靶核酸的用途和试剂盒
EP3967768A1 (en) 2015-03-13 2022-03-16 Life Technologies Corporation Compositions for small rna capture, detection and quantification
CN104975078A (zh) * 2015-05-18 2015-10-14 上海市农业科学院 一种检测转基因植物中靶标siRNA池的方法
TWI676679B (zh) * 2015-06-02 2019-11-11 奎克生技光電股份有限公司 核苷酸序列、通用反向引子、通用反轉錄引子、引子設計方法及miRNA檢測方法
HUE050491T2 (hu) 2015-11-10 2020-12-28 Eurofins Lifecodexx Gmbh Magzati kromoszomális aneuploidiák kimutatása olyan DNS régiókat alkalmazva, amelyek különbözõképpen vannak metilezve a magzat és a terhes nõstény között
WO2017112666A1 (en) 2015-12-21 2017-06-29 Somagenics, Inc. Methods of library construction for polynucleotide sequencing
JP6577660B2 (ja) 2016-03-18 2019-09-18 株式会社東芝 試料中の複数種類の短鎖核酸の検出方法、組み合わせ分析キットおよび分析キット供給管理方法
CZ2016248A3 (cs) * 2016-04-29 2017-12-20 Ústav molekulární genetiky AV ČR, v.v.i. Způsob detekce specifických mikroRNA pomocí templátové DNA a molekula templátové DNA pro použití při tomto způsobu
WO2019011875A1 (en) * 2017-07-11 2019-01-17 Qiagen Gmbh QUANTIFICATION OF ANTISENSE OLIGONUCLEOTIDES (ASOS)
US20220298555A1 (en) * 2018-12-20 2022-09-22 Roche Molecular Systems, Inc. Detection of target nucleic acid by solid-phase molography
WO2020239822A1 (en) * 2019-05-27 2020-12-03 The European Molecular Biology Laboratory Nucleic acid construct binding to influenza polymerase pb1 rna synthesis active site
EP3901286A1 (en) * 2020-04-24 2021-10-27 Mirnax Biosens, S.L. Bivalent reverse primer
ES3030914T3 (en) * 2019-11-04 2025-07-02 Mirnax Biosens S L Bivalent reverse primer
CN111665351B (zh) * 2020-06-20 2021-12-03 江南大学 一种快速、特异性测定rna含量的方法
CN113322523B (zh) * 2021-06-17 2024-03-19 翌圣生物科技(上海)股份有限公司 Rna快速建库方法及其应用
CN116200492B (zh) * 2022-11-08 2025-04-01 浙江大学 一种基于探针锚定双链的小核酸检测试剂盒及其应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6695600A (en) * 1999-07-19 2001-02-05 Cambridge University Technical Services Limited A method for amplifying low abundance nucleic acid sequences and means for performing said method
WO2002057479A2 (en) * 2000-12-27 2002-07-25 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US20040235005A1 (en) * 2002-10-23 2004-11-25 Ernest Friedlander Methods and composition for detecting targets
US7217807B2 (en) * 2002-11-26 2007-05-15 Rosetta Genomics Ltd Bioinformatically detectable group of novel HIV regulatory genes and uses thereof
JP4230457B2 (ja) * 2002-12-18 2009-02-25 サード・ウェーブ・テクノロジーズ・インク 低分子核酸の検出方法
EP1644533A4 (en) * 2003-07-02 2007-11-14 Perkinelmer Las Inc ANALYSIS AND METHOD OF MARKING AND DETECTING RNA MICROSEQUENCES AND SMALL INTERFERENCE RNA SEQUENCES
JP2007512805A (ja) * 2003-10-14 2007-05-24 ノバルティス　アクチエンゲゼルシャフト オリゴヌクレオチドマイクロアレイ

Also Published As

Publication number Publication date
CN1961081B (zh) 2012-09-05
JP5192229B2 (ja) 2013-05-08
ES2380923T3 (es) 2012-05-21
WO2005098029A3 (en) 2005-12-15
EP1735459A2 (en) 2006-12-27
CA2562390A1 (en) 2005-10-20
JP2007532100A (ja) 2007-11-15
CN1961081A (zh) 2007-05-09
CA2562390C (en) 2014-12-02
EP1735459B1 (en) 2012-01-25
WO2005098029A2 (en) 2005-10-20
ATE542918T1 (de) 2012-02-15

Similar Documents

Publication Publication Date Title
DK1735459T3 (da) Fremgangsmåder til kvantificering af microRNA'er og små interfererende RNA'er
WO2008040355A3 (en) Novel methods for quantification of micrornas and small interfering rnas
Lopez et al. Biomarker discovery: quantification of microRNAs and other small non-coding RNAs using next generation sequencing
DK1597391T3 (da) Anvendelse af intron-RNA til måling af genekspression
Ichii et al. Altered expression of microRNA miR-146a correlates with the development of chronic renal inflammation
Courts et al. Micro-RNA–a potential for forensic science?
WO2006069584A3 (en) NOVEL OLIGONUCLEOTIDE COMPOSITIONS AND PROBE SEQUENCES USEFUL FOR DETECTION AND ANALYSIS OF microRNAs AND THEIR TARGET mRNAs
EP1750771A4 (en) PANCREAS MICRO RNA AND METHOD OF SUPPRESSING IT
Badal et al. MicroRNAs and their applications in kidney diseases
DE60324188D1 (de) Verfahren zur verwendung von riboprimern zur strangverdrängungsreplikation von zielsequenzen
WO2014205555A1 (en) Methods and uses for diagnosis and treatment of prostate cancer
WO2006119266A3 (en) Human micrornas and methods for inhibiting same
ATE437962T1 (de) Verfahren für die behandlung von proliferativen erkrankungen von brustzellen
WO2004111190A3 (en) IN VIVO HIGH THROUGHPUT SELECTION OF EFFECTIVE RNAi PROBES
DE602006017192D1 (de) Verfahren zur herstellung von oligonukleotiden
MX2009002462A (es) Siarn y metodos de manufactura del mismo.
WO2008094516A8 (en) Multi-targeting short interfering rnas
ATE505562T1 (de) Doppel-oligonukleotid- nukleinsäurenachweisverfahren
BRPI0415302A (pt) uso de polimorfismos genéticos que se associam a eficácia de tratamento de doença inflamatória
CY1115586T1 (el) Μεθοδος για την απομονωση mrna απο μονιμοποιημενο με φορμαλινη εμπεδωμενο με παραφινη ιστο
Hong et al. Identification and characterization of novel and conserved microRNAs in several tissues of the Chinese rare minnow (Gobiocypris rarus) based on illumina deep sequencing technology
WO2006076674A3 (en) Deprotection and purification of oligonucleotides and their derivatives
ATE446385T1 (de) Polymorphismen im nod2/card15 gen
Wu et al. Simple and nonradioactive detection of microRNAs using digoxigenin (DIG)-labeled probes with high sensitivity
Peng et al. A spatiotemporally resolved atlas of mRNA decay in the C. elegans embryo reveals differential regulation of mRNA stability across stages and cell types