JP5095386B2 - プラスミド精製 - Google Patents

プラスミド精製 Download PDF

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Publication number
JP5095386B2
JP5095386B2 JP2007500711A JP2007500711A JP5095386B2 JP 5095386 B2 JP5095386 B2 JP 5095386B2 JP 2007500711 A JP2007500711 A JP 2007500711A JP 2007500711 A JP2007500711 A JP 2007500711A JP 5095386 B2 JP5095386 B2 JP 5095386B2
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JP
Japan
Prior art keywords
plasmid
separation matrix
matrix
dna
separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2007500711A
Other languages
English (en)
Japanese (ja)
Other versions
JP2007525222A5 (enExample
JP2007525222A (ja
Inventor
ヴァン・アルスティン，ジェイムズ
ベルグストロム，ヤン
リンドストロム，ダグ
スタッドラー，ホアキム
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Amersham Bioscience AB
GE Healthcare Bio Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Bioscience AB, GE Healthcare Bio Sciences Corp filed Critical Amersham Bioscience AB
Publication of JP2007525222A publication Critical patent/JP2007525222A/ja
Publication of JP2007525222A5 publication Critical patent/JP2007525222A5/ja
Application granted granted Critical
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Saccharide Compounds (AREA)
JP2007500711A 2004-02-26 2005-02-21 プラスミド精製 Expired - Fee Related JP5095386B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0400490-9 2004-02-26
SE0400490A SE0400490D0 (sv) 2004-02-26 2004-02-26 Plasmid purification
PCT/SE2005/000229 WO2005083080A1 (en) 2004-02-26 2005-02-21 Plasmid purification

Publications (3)

Publication Number Publication Date
JP2007525222A JP2007525222A (ja) 2007-09-06
JP2007525222A5 JP2007525222A5 (enExample) 2008-04-10
JP5095386B2 true JP5095386B2 (ja) 2012-12-12

Family

ID=32067270

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2007500711A Expired - Fee Related JP5095386B2 (ja) 2004-02-26 2005-02-21 プラスミド精製

Country Status (6)

Country Link
US (1) US8093373B2 (enExample)
EP (1) EP1718744B1 (enExample)
JP (1) JP5095386B2 (enExample)
CN (1) CN1922319A (enExample)
SE (1) SE0400490D0 (enExample)
WO (1) WO2005083080A1 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HRP20170233T1 (hr) * 2007-05-23 2017-06-02 Vgxi, Inc. Sastav koji sadrži visoku koncentraciju biološki aktivnih molekula i postupci za njegovu pripravu
WO2010027696A1 (en) * 2008-08-25 2010-03-11 Ge Healthcare Bio-Sciences Corp. Simple load and elute process for purification of genomic dna
CN103392128B (zh) * 2011-02-10 2016-05-04 积水医疗株式会社 离子交换色谱法用填充剂以及核酸链的分离检测方法
US9647476B2 (en) 2014-09-16 2017-05-09 Navitas Semiconductor Inc. Integrated bias supply, reference and bias current circuits for GaN devices
KR101751700B1 (ko) 2014-09-18 2017-06-30 한국과학기술연구원 전하를 띄는 다공성 구조체
WO2016192012A1 (zh) * 2015-06-01 2016-12-08 易初丽 一种质粒纯化系统
CN106884011B (zh) * 2015-12-16 2020-11-17 固安鼎泰海规生物科技有限公司 一种规模化质粒纯化的联合液相层析分离方法
WO2018063980A1 (en) 2016-09-29 2018-04-05 Bio-Rad Laboratories, Inc. Agarose-filled ceramic apatite
CN110462400B (zh) 2017-03-28 2022-06-21 相达生物科技国际有限公司 用于在液体活检中准确诊断疾病靶向生物标记的方法
CN119425158A (zh) 2017-06-01 2025-02-14 相达生物科技美国有限公司 用于多孔材料中双水相分离的相分离行为改性剂
US11609232B2 (en) 2017-09-01 2023-03-21 Phase Diagnostics, Inc. Method and device of using aqueous two-phase systems (ATPS) for enhancing diagnostics for sexually transmitted infections
JP7323532B2 (ja) 2017-09-18 2023-08-08 フェーズ サイエンティフィック インターナショナル リミテッド 短い核酸断片の単離、精製及び／又は濃縮のために水性二相系を使用する方法
US12043826B2 (en) 2018-01-19 2024-07-23 Phase Scientific International, Ltd. Spontaneous nucleic acid purification and concentration in a single step
US11643646B2 (en) 2018-01-19 2023-05-09 Phase Scientific International, Ltd. Method for isolating and purifying nucleic acids using a solid-liquid phase system
WO2019144016A1 (en) 2018-01-19 2019-07-25 Yin To Chiu Method of isolating exosomes using encapsulation and aqueous micellar system
CN111088248B (zh) * 2019-12-23 2020-10-16 广州湾区生物科技有限公司 一种用于提取无内毒素质粒的磁性材料及其使用方法
CN115135392A (zh) 2020-02-19 2022-09-30 生物辐射实验室股份有限公司 制备聚合物填充的色谱树脂的方法
AU2021239220B2 (en) 2020-03-20 2024-11-14 Phase Scientific International, Ltd. Compositions and methods for ribonucleic acid extraction

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9601368D0 (sv) 1996-04-11 1996-04-11 Pharmacia Biotech Ab Process for the production of a porous cross-linked polysaccharide gel
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US6011148A (en) 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
JP4425994B2 (ja) 1996-08-30 2010-03-03 ウプフロント クロマトグラフィー アクティーゼルスカブ 免疫グロブリンの単離
SE9700769D0 (sv) 1997-03-04 1997-03-04 Pharmacia Biotech Ab Matriser för separation och separation som utnyttjar matriserna
US6214586B1 (en) 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
DE69922740T2 (de) 1998-05-11 2005-12-08 Tosoh Corp., Shinnanyo Methode zur Trennung von Nucleinsäuren mittels Flüssigchromatographie
WO1999063076A1 (en) 1998-06-01 1999-12-09 The Immune Response Corporation Novel method of large scale plasmid purification
EP0987327A1 (en) 1998-09-14 2000-03-22 QIAGEN GmbH Novel method for purifying covalently closed circular DNA
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
BR0012627A (pt) 1999-07-23 2002-04-09 Genentech Inc Processo para purificar o dna de plasmìdeo de células procarióticas mediante o emprego de filtração de fluxo tangencial
SE9904272D0 (sv) * 1999-11-25 1999-11-25 Amersham Pharm Biotech Ab A method for selective removal of a substance from samples containing compounds having nucleic acid structure
DE10009982A1 (de) * 2000-03-03 2001-09-06 Qiagen Gmbh Polymere Anionenaustauscher und deren Verwendung in chromatographischen Verfahren
AU2003256787A1 (en) * 2002-07-26 2004-02-16 Applera Corporation Petal-array support for use with microplates

Also Published As

Publication number Publication date
CN1922319A (zh) 2007-02-28
US8093373B2 (en) 2012-01-10
EP1718744B1 (en) 2013-11-13
EP1718744A1 (en) 2006-11-08
WO2005083080A1 (en) 2005-09-09
US20070161000A1 (en) 2007-07-12
JP2007525222A (ja) 2007-09-06
SE0400490D0 (sv) 2004-02-26

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