CN1922319A - 质粒纯化 - Google Patents

质粒纯化 Download PDF

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Publication number
CN1922319A
CN1922319A CNA2005800059786A CN200580005978A CN1922319A CN 1922319 A CN1922319 A CN 1922319A CN A2005800059786 A CNA2005800059786 A CN A2005800059786A CN 200580005978 A CN200580005978 A CN 200580005978A CN 1922319 A CN1922319 A CN 1922319A
Authority
CN
China
Prior art keywords
plasmid
matrix
separation matrix
plasmids
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CNA2005800059786A
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English (en)
Chinese (zh)
Inventor
J·范阿尔斯泰恩
J·贝里斯特伦
D·林德斯特伦
J·施塔德勒
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
GE Healthcare Bio Sciences AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare Bio Sciences AB filed Critical GE Healthcare Bio Sciences AB
Publication of CN1922319A publication Critical patent/CN1922319A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction

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  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Saccharide Compounds (AREA)
CNA2005800059786A 2004-02-26 2005-02-21 质粒纯化 Pending CN1922319A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE04004909 2004-02-26
SE0400490A SE0400490D0 (sv) 2004-02-26 2004-02-26 Plasmid purification

Publications (1)

Publication Number Publication Date
CN1922319A true CN1922319A (zh) 2007-02-28

Family

ID=32067270

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA2005800059786A Pending CN1922319A (zh) 2004-02-26 2005-02-21 质粒纯化

Country Status (6)

Country Link
US (1) US8093373B2 (enExample)
EP (1) EP1718744B1 (enExample)
JP (1) JP5095386B2 (enExample)
CN (1) CN1922319A (enExample)
SE (1) SE0400490D0 (enExample)
WO (1) WO2005083080A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105567672A (zh) * 2007-05-23 2016-05-11 Vgxi公司 包含高浓度的生物活性分子的组合物及其制备工艺
WO2016192012A1 (zh) * 2015-06-01 2016-12-08 易初丽 一种质粒纯化系统
CN106884011A (zh) * 2015-12-16 2017-06-23 固安鼎泰海规生物科技有限公司 一种规模化质粒纯化的联合液相层析分离方法
CN111088248A (zh) * 2019-12-23 2020-05-01 广州湾区生物科技有限公司 一种用于提取无内毒素质粒的磁性材料及其使用方法

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2315839A4 (en) * 2008-08-25 2012-03-21 Ge Healthcare Bio Sciences EASY LOADING AND ELONGATION METHOD FOR CLEANING GENOMIC DNA
EP2674753B1 (en) * 2011-02-10 2020-05-13 Sekisui Medical Co., Ltd. Method for separating and detecting a nucleic acid strand
US9647476B2 (en) 2014-09-16 2017-05-09 Navitas Semiconductor Inc. Integrated bias supply, reference and bias current circuits for GaN devices
KR101751700B1 (ko) 2014-09-18 2017-06-30 한국과학기술연구원 전하를 띄는 다공성 구조체
CN109803736B (zh) 2016-09-29 2022-02-08 生物辐射实验室股份有限公司 琼脂糖填充的陶瓷磷灰石
EP4234710A3 (en) 2017-03-28 2023-11-01 Phase Scientific International, Ltd. Method for accurate diagnosis of a disease targeting biomarkers in liquid biopsy
EP3595791B1 (en) 2017-06-01 2024-05-15 Phase Diagnostics, Inc. Phase separation behavior modifying agent dextran for aqueous two-phase separation within porous fibreglass material
WO2019046563A1 (en) 2017-09-01 2019-03-07 Phase Diagnostics, Inc. METHOD AND DEVICE FOR USING AQUEOUS BIPHASE SYSTEMS (ATPS) TO IMPROVE THE DIAGNOSIS OF SEXUALLY TRANSMITTED INFECTIONS
CN111356770B (zh) 2017-09-18 2023-12-08 相达生物科技国际有限公司 使用双水相系统来分离、纯化和/或浓缩短链核酸片段的方法
WO2019144016A1 (en) 2018-01-19 2019-07-25 Yin To Chiu Method of isolating exosomes using encapsulation and aqueous micellar system
US11643646B2 (en) * 2018-01-19 2023-05-09 Phase Scientific International, Ltd. Method for isolating and purifying nucleic acids using a solid-liquid phase system
WO2019143895A1 (en) 2018-01-19 2019-07-25 Yin To Chiu Spontaneous nucleic acid purification and concentration in a single step
CN115135392A (zh) 2020-02-19 2022-09-30 生物辐射实验室股份有限公司 制备聚合物填充的色谱树脂的方法
US12319909B2 (en) 2020-03-20 2025-06-03 Phase Scientific International, Ltd. Compositions and methods for ribonucleic acid extraction

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9601368D0 (sv) 1996-04-11 1996-04-11 Pharmacia Biotech Ab Process for the production of a porous cross-linked polysaccharide gel
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US6011148A (en) 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
DE69739567D1 (de) 1996-08-30 2009-10-15 Upfront Chromatography As Kope Isolierung von Immunoglobulinen
SE9700769D0 (sv) 1997-03-04 1997-03-04 Pharmacia Biotech Ab Matriser för separation och separation som utnyttjar matriserna
US6214586B1 (en) 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
US6441160B2 (en) 1998-05-11 2002-08-27 Tosoh Corporation Separating plasmids from contaminants using hydrophobic or hydrophobic and ion exchange chromatography
WO1999063076A1 (en) * 1998-06-01 1999-12-09 The Immune Response Corporation Novel method of large scale plasmid purification
EP0987327A1 (en) 1998-09-14 2000-03-22 QIAGEN GmbH Novel method for purifying covalently closed circular DNA
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
NZ516641A (en) 1999-07-23 2003-07-25 Genentech Inc Method for RNase- and organic solvent-free plasmid DNA purification using tangential flow filtration
SE9904272D0 (sv) * 1999-11-25 1999-11-25 Amersham Pharm Biotech Ab A method for selective removal of a substance from samples containing compounds having nucleic acid structure
DE10009982A1 (de) 2000-03-03 2001-09-06 Qiagen Gmbh Polymere Anionenaustauscher und deren Verwendung in chromatographischen Verfahren
AU2003254174A1 (en) * 2002-07-26 2004-02-16 Applera Corporation Size-exclusion ion-exchange particles

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105567672A (zh) * 2007-05-23 2016-05-11 Vgxi公司 包含高浓度的生物活性分子的组合物及其制备工艺
WO2016192012A1 (zh) * 2015-06-01 2016-12-08 易初丽 一种质粒纯化系统
CN106884011A (zh) * 2015-12-16 2017-06-23 固安鼎泰海规生物科技有限公司 一种规模化质粒纯化的联合液相层析分离方法
CN111088248A (zh) * 2019-12-23 2020-05-01 广州湾区生物科技有限公司 一种用于提取无内毒素质粒的磁性材料及其使用方法
CN111088248B (zh) * 2019-12-23 2020-10-16 广州湾区生物科技有限公司 一种用于提取无内毒素质粒的磁性材料及其使用方法

Also Published As

Publication number Publication date
WO2005083080A1 (en) 2005-09-09
EP1718744B1 (en) 2013-11-13
JP5095386B2 (ja) 2012-12-12
US20070161000A1 (en) 2007-07-12
EP1718744A1 (en) 2006-11-08
SE0400490D0 (sv) 2004-02-26
US8093373B2 (en) 2012-01-10
JP2007525222A (ja) 2007-09-06

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