JP5042431B2 - Ｂｉｆｉｄｏｂａｃｔｅｒｉｕｍから単離された新たな酵素 - Google Patents
Ｂｉｆｉｄｏｂａｃｔｅｒｉｕｍから単離された新たな酵素 Download PDFInfo
- Publication number
- JP5042431B2 JP5042431B2 JP2001587113A JP2001587113A JP5042431B2 JP 5042431 B2 JP5042431 B2 JP 5042431B2 JP 2001587113 A JP2001587113 A JP 2001587113A JP 2001587113 A JP2001587113 A JP 2001587113A JP 5042431 B2 JP5042431 B2 JP 5042431B2
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- Prior art keywords
- enzyme
- cell
- lactose
- bifidobacterium
- activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- 125000003275 alpha amino acid group Chemical group 0.000 claims description 4
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- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 description 1
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
- C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Dairy Products (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US20715400P | 2000-05-26 | 2000-05-26 | |
| US60/207,154 | 2000-05-26 | ||
| PCT/DK2001/000366 WO2001090317A2 (fr) | 2000-05-26 | 2001-05-25 | Nouvelle enzyme isolee a partie de bifidobacterium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2003534007A JP2003534007A (ja) | 2003-11-18 |
| JP5042431B2 true JP5042431B2 (ja) | 2012-10-03 |
Family
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Family Applications (1)
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| EP (1) | EP1283876B1 (fr) |
| JP (1) | JP5042431B2 (fr) |
| KR (2) | KR20080045766A (fr) |
| AT (1) | ATE352611T1 (fr) |
| AU (2) | AU6206801A (fr) |
| DE (1) | DE60126269T2 (fr) |
| DK (1) | DK1283876T3 (fr) |
| NZ (1) | NZ522623A (fr) |
| WO (1) | WO2001090317A2 (fr) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7250496B2 (en) * | 2002-11-14 | 2007-07-31 | Rosetta Genomics Ltd. | Bioinformatically detectable group of novel regulatory genes and uses thereof |
| GB0315266D0 (en) * | 2003-06-30 | 2003-08-06 | Clasado Inc | Novel galactooligosaccharide composition and the preparation thereof |
| ATE360682T1 (de) * | 2003-06-30 | 2007-05-15 | Clasado Inc | Neue galactooligosaccharidzusammensetzung und herstellung davon |
| GB0525857D0 (en) | 2005-12-20 | 2006-02-01 | Product and process | |
| GB0601901D0 (en) | 2006-01-31 | 2006-03-08 | Product and Process | |
| GB0606112D0 (en) | 2006-03-28 | 2006-05-03 | Product and process | |
| US8071353B2 (en) | 2006-08-18 | 2011-12-06 | Nestec S.A. | Genetic remodeling in Bifidobacterium |
| US8361756B2 (en) * | 2006-09-15 | 2013-01-29 | The Regents Of The University Of California | Bifidobacterial gene sequences and their use |
| WO2009071539A1 (fr) | 2007-12-03 | 2009-06-11 | Novozymes A/S | Procédé de fabrication d'un produit laitier |
| GB0809921D0 (en) * | 2008-05-30 | 2008-07-09 | Clasado Inc | Product and process therefor |
| US20090297660A1 (en) * | 2008-06-02 | 2009-12-03 | Kraft Food Holdings, Inc. | Cheese Products Containing Galacto-Oligosaccharides And Having Reduced Lactose Levels |
| US11065268B2 (en) | 2009-05-27 | 2021-07-20 | Clasado Research Services Limited | Method of preventing diarrhoea |
| US20110189342A1 (en) * | 2010-02-01 | 2011-08-04 | Jeong Hea-Seok | High-purity galactooligosaccharides and uses thereof |
| US9107440B2 (en) | 2010-03-29 | 2015-08-18 | Dupont Nutrition Biosciences Aps | Polypeptides having transgalactosylating activity |
| JP2017018135A (ja) * | 2010-07-19 | 2017-01-26 | アルラ フーズ アムバＡｒｌａ Ｆｏｏｄｓ Ａｍｂａ | ガラクトオリゴ糖含有組成物およびそれを生産する方法 |
| MX348325B (es) * | 2010-07-19 | 2017-06-06 | Arla Foods Amba | Composicion que contiene galacto-oligosacarido y un metodo para producirla. |
| EP2620506A1 (fr) * | 2012-01-25 | 2013-07-31 | Arla Foods Amba | Procédé de production de composition contenant des galacto-oligosaccharides |
| JP6641179B2 (ja) * | 2012-06-08 | 2020-02-05 | デュポン ニュートリション バイオサイエンシス エーピーエス | トランスガラクトシル化活性を有するポリペプチド |
| KR20150093775A (ko) | 2012-12-07 | 2015-08-18 | 노쓰 캐롤라이나 스테이트 유니버시티 | 베타 헥소실-전이효소 및 이의 이용 |
| CA2913393A1 (fr) | 2013-05-24 | 2014-11-27 | General Mills, Inc. | Produits alimentaires a base de lactoserum de yogourt |
| AU2014363517B2 (en) * | 2013-12-11 | 2018-06-28 | International N&H Denmark Aps | A method for preparing a dairy product having a stable content of galacto-oligosaccharide(s) |
| WO2015107050A1 (fr) * | 2014-01-14 | 2015-07-23 | Dsm Ip Assets B.V. | Variants enzymatiques améliorés de lactase provenant de kluyveromyces lactis |
| WO2015132402A1 (fr) * | 2014-03-06 | 2015-09-11 | Arla Foods Amba | Produits laitiers à teneur réduite en lactose contenant des galacto-oligosaccharides et des monosaccharides et procédé de production |
| EP3214942B1 (fr) | 2014-11-07 | 2021-02-24 | DuPont Nutrition Biosciences ApS | Cellule hôte recombinante exprimant bêta-galactosidase et/ou une activité de transgalactosylation déficient en cellulase, mannanase et pectinase. |
| EP3215628A1 (fr) * | 2014-11-07 | 2017-09-13 | DuPont Nutrition Biosciences ApS | Procédé de génération d'un saccharide, contenant une fraction galactose et une fraction fructose, utilisant une enzyme à activité de transgalactosylation |
| WO2017167847A1 (fr) | 2016-03-31 | 2017-10-05 | Dsm Ip Assets B.V. | Production de lait à faible teneur en lactose |
| EP3435771A1 (fr) | 2016-03-31 | 2019-02-06 | DSM IP Assets B.V. | Composition enzymatique et préparation d'un produit laitier ayant des propriétés améliorées |
| WO2017167849A1 (fr) | 2016-03-31 | 2017-10-05 | Dsm Ip Assets B.V. | Composition enzymatique et préparation d'un produit laitier aux propriétés améliorées |
| PT3228194T (pt) | 2016-04-07 | 2021-04-05 | Dsm Ip Assets Bv | Produto lácteo fermentado com diacetilo produzido com auxílio de lactase |
| WO2018187524A1 (fr) | 2017-04-07 | 2018-10-11 | Dupont Nutrition Biosciences Aps | CELLULES HÔTES DE BACILLUS PRODUISANT DES β-GALACTOSIDASES ET DES LACTASES EN L'ABSENCE D'ACTIVITÉ SECONDAIRE P-NITROBENZYLESTÉRASE |
| EP3624593A1 (fr) | 2017-05-15 | 2020-03-25 | Novozymes A/S | Compositions de bêta-galactosidase glycosylées présentant une activité de transgalactosylation améliorée |
| RU2698460C1 (ru) * | 2019-03-28 | 2019-08-27 | Общество с ограниченной ответственностью "Международный Биотехнологический Центр "Генериум" | Способ получения рекомбинантной в-1,4-галактозидазы bgaa из streptococcus pneumoniae |
| EP4110909A1 (fr) * | 2020-02-26 | 2023-01-04 | Novozymes A/S | Variants polypeptidiques |
| WO2023083971A1 (fr) | 2021-11-12 | 2023-05-19 | Novozymes A/S | Procédé de production d'un produit laitier |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5153128A (en) * | 1990-03-16 | 1992-10-06 | Suntory Limited | Heat-resistant β-galactosyltransferase, its production process and its use |
| JPH05146296A (ja) * | 1991-11-27 | 1993-06-15 | Yakult Honsha Co Ltd | β−ガラクトシダーゼ遺伝子を含むＤＮＡ断片及び該ＤＮＡ断片を組み込んだプラスミド |
| GB9221163D0 (en) * | 1992-10-08 | 1992-11-25 | Nobipol And Protan Biopolymer | Dna compounds |
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2001
- 2001-05-25 EP EP01936043A patent/EP1283876B1/fr not_active Expired - Lifetime
- 2001-05-25 JP JP2001587113A patent/JP5042431B2/ja not_active Expired - Lifetime
- 2001-05-25 WO PCT/DK2001/000366 patent/WO2001090317A2/fr active IP Right Grant
- 2001-05-25 DE DE60126269T patent/DE60126269T2/de not_active Expired - Lifetime
- 2001-05-25 AU AU6206801A patent/AU6206801A/xx active Pending
- 2001-05-25 AU AU2001262068A patent/AU2001262068B2/en not_active Expired
- 2001-05-25 KR KR1020087010589A patent/KR20080045766A/ko not_active Application Discontinuation
- 2001-05-25 NZ NZ522623A patent/NZ522623A/en not_active IP Right Cessation
- 2001-05-25 KR KR1020027015920A patent/KR100878968B1/ko active IP Right Grant
- 2001-05-25 AT AT01936043T patent/ATE352611T1/de not_active IP Right Cessation
- 2001-05-25 DK DK01936043T patent/DK1283876T3/da active
- 2001-05-29 US US09/865,621 patent/US6555348B2/en not_active Expired - Lifetime
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Also Published As
| Publication number | Publication date |
|---|---|
| EP1283876A2 (fr) | 2003-02-19 |
| AU2001262068B2 (en) | 2006-07-06 |
| AU6206801A (en) | 2001-12-03 |
| EP1283876B1 (fr) | 2007-01-24 |
| JP2003534007A (ja) | 2003-11-18 |
| US7081355B2 (en) | 2006-07-25 |
| KR100878968B1 (ko) | 2009-01-19 |
| ATE352611T1 (de) | 2007-02-15 |
| US6555348B2 (en) | 2003-04-29 |
| WO2001090317A3 (fr) | 2002-04-25 |
| US20020086358A1 (en) | 2002-07-04 |
| DK1283876T3 (da) | 2007-02-19 |
| DE60126269T2 (de) | 2007-06-21 |
| DE60126269D1 (de) | 2007-03-15 |
| WO2001090317A2 (fr) | 2001-11-29 |
| US20030162280A1 (en) | 2003-08-28 |
| NZ522623A (en) | 2003-10-31 |
| KR20030016275A (ko) | 2003-02-26 |
| KR20080045766A (ko) | 2008-05-23 |
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