WO2017167849A1 - Composition enzymatique et préparation d'un produit laitier aux propriétés améliorées - Google Patents

Composition enzymatique et préparation d'un produit laitier aux propriétés améliorées Download PDF

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Publication number
WO2017167849A1
WO2017167849A1 PCT/EP2017/057490 EP2017057490W WO2017167849A1 WO 2017167849 A1 WO2017167849 A1 WO 2017167849A1 EP 2017057490 W EP2017057490 W EP 2017057490W WO 2017167849 A1 WO2017167849 A1 WO 2017167849A1
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WO
WIPO (PCT)
Prior art keywords
activity
seq
lactase
sequence
enzyme composition
Prior art date
Application number
PCT/EP2017/057490
Other languages
English (en)
Inventor
Petrus Jacobus Theodorus Dekker
Cornelis Marinus Muijlwijk
Marten Aalt PAASMAN
Original Assignee
Dsm Ip Assets B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets B.V. filed Critical Dsm Ip Assets B.V.
Priority to EP17714719.6A priority Critical patent/EP3435772A1/fr
Publication of WO2017167849A1 publication Critical patent/WO2017167849A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G9/00—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor
    • A23G9/32—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds
    • A23G9/36—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
    • A23C9/1206—Lactose hydrolysing enzymes, e.g. lactase, beta-galactosidase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/137—Thickening substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G9/00—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor
    • A23G9/32—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds
    • A23G9/40—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds characterised by the dairy products used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01108—Lactase (3.2.1.108)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C2260/00—Particular aspects or types of dairy products
    • A23C2260/15—Frozen dairy products

Abstract

La présente invention concerne un produit laitier pouvant être obtenu par un procédé qui comprend : a) la fourniture d'un substrat à base de lait comprenant du lactose ; et b) le traitement dudit substrat avec une composition enzymatique comprenant une activité de la lactase, au moins une partie de l'activité de la lactase étant fournie par un polypeptide ayant une séquence d'acides aminés qui comprend la séquence définie dans SEQ ID NO: 1 ou SEQ ID NO : 2, une version tronquée de l'une ou l'autre desdites séquences ou une séquence ayant au moins environ 60 % d'identité de séquence avec l'une quelconque desdites séquences, et la composition enzymatique ayant une activité de l'arylsulfatase réduite, une activité de l'invertase, une activité de la lipase et/ou une activité de l'amylase.
PCT/EP2017/057490 2016-03-31 2017-03-30 Composition enzymatique et préparation d'un produit laitier aux propriétés améliorées WO2017167849A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP17714719.6A EP3435772A1 (fr) 2016-03-31 2017-03-30 Composition enzymatique et préparation d'un produit laitier aux propriétés améliorées

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662316389P 2016-03-31 2016-03-31
US62/316389 2016-03-31

Publications (1)

Publication Number Publication Date
WO2017167849A1 true WO2017167849A1 (fr) 2017-10-05

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2017/057490 WO2017167849A1 (fr) 2016-03-31 2017-03-30 Composition enzymatique et préparation d'un produit laitier aux propriétés améliorées

Country Status (2)

Country Link
EP (1) EP3435772A1 (fr)
WO (1) WO2017167849A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020127511A1 (fr) 2018-12-19 2020-06-25 Firmenich Sa Compositions laitières édulcorées comprenant des produits d'hydrolyse du lactose et des glycosides de stéviol naturels glucosylés
WO2022268875A1 (fr) 2021-06-23 2022-12-29 Firmenich Sa Compositions modifiant l'arôme contenant de la lactase

Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990014423A1 (fr) 1989-05-18 1990-11-29 The Infergene Company Transformation de microorganismes
EP0481008A1 (fr) 1989-07-07 1992-04-22 Unilever Plc Procede de preparation d'une proteine a partir d'un champignon transforme par integration multicopie d'un vecteur d'expression
EP0635574A1 (fr) 1993-07-23 1995-01-25 Gist-Brocades N.V. Souches récombinantes dépourvues de marqueurs de sélection: procédé pour leur obtention et utilisation de ces souches
WO1998046772A2 (fr) 1997-04-11 1998-10-22 Dsm N.V. Transformation genetique comme outil pour la construction de champignons filamenteux industriels de recombinaison
WO1999060102A2 (fr) 1998-05-19 1999-11-25 Dsm N.V. Perfectionnement d'un procede de production de cephalosporines
WO2000037671A2 (fr) 1998-12-22 2000-06-29 Dsm N.V. Production in vivo amelioree de cephalosporines
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
WO2001090317A2 (fr) 2000-05-26 2001-11-29 Arla Foods Amba Nouvelle enzyme isolee a partie de bifidobacterium
WO2009071538A2 (fr) 2007-12-04 2009-06-11 Shell Internationale Research Maatschappij B.V. Procédé et appareil permettant de refroidir et/ou de liquéfier un flux d'hydrocarbure
WO2009071539A1 (fr) 2007-12-03 2009-06-11 Novozymes A/S Procédé de fabrication d'un produit laitier
AU2013200779A1 (en) * 2005-11-28 2013-02-28 Dsm Ip Assets B.V. Enzyme preparations yielding a clean taste
WO2013182686A1 (fr) * 2012-06-08 2013-12-12 Dupont Nutrition Biosciences Aps Polypeptides présentant une activité de transgalactosylation
WO2014184189A2 (fr) 2013-05-14 2014-11-20 Novozymes A/S Procédé de saccharification et de fermentation simultanées de perméat de lactosérum
WO2015132349A1 (fr) * 2014-03-05 2015-09-11 Dsm Ip Assets B.V. Compositions de lactase liquides

Patent Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990014423A1 (fr) 1989-05-18 1990-11-29 The Infergene Company Transformation de microorganismes
EP0481008A1 (fr) 1989-07-07 1992-04-22 Unilever Plc Procede de preparation d'une proteine a partir d'un champignon transforme par integration multicopie d'un vecteur d'expression
EP0635574A1 (fr) 1993-07-23 1995-01-25 Gist-Brocades N.V. Souches récombinantes dépourvues de marqueurs de sélection: procédé pour leur obtention et utilisation de ces souches
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
WO1998046772A2 (fr) 1997-04-11 1998-10-22 Dsm N.V. Transformation genetique comme outil pour la construction de champignons filamenteux industriels de recombinaison
WO1999060102A2 (fr) 1998-05-19 1999-11-25 Dsm N.V. Perfectionnement d'un procede de production de cephalosporines
WO2000037671A2 (fr) 1998-12-22 2000-06-29 Dsm N.V. Production in vivo amelioree de cephalosporines
WO2001090317A2 (fr) 2000-05-26 2001-11-29 Arla Foods Amba Nouvelle enzyme isolee a partie de bifidobacterium
AU2013200779A1 (en) * 2005-11-28 2013-02-28 Dsm Ip Assets B.V. Enzyme preparations yielding a clean taste
WO2009071539A1 (fr) 2007-12-03 2009-06-11 Novozymes A/S Procédé de fabrication d'un produit laitier
WO2009071538A2 (fr) 2007-12-04 2009-06-11 Shell Internationale Research Maatschappij B.V. Procédé et appareil permettant de refroidir et/ou de liquéfier un flux d'hydrocarbure
WO2013182686A1 (fr) * 2012-06-08 2013-12-12 Dupont Nutrition Biosciences Aps Polypeptides présentant une activité de transgalactosylation
WO2014184189A2 (fr) 2013-05-14 2014-11-20 Novozymes A/S Procédé de saccharification et de fermentation simultanées de perméat de lactosérum
WO2015132349A1 (fr) * 2014-03-05 2015-09-11 Dsm Ip Assets B.V. Compositions de lactase liquides

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J. MOL. BIOL., vol. 215, 1990, pages 403 - 10
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020127511A1 (fr) 2018-12-19 2020-06-25 Firmenich Sa Compositions laitières édulcorées comprenant des produits d'hydrolyse du lactose et des glycosides de stéviol naturels glucosylés
WO2022268875A1 (fr) 2021-06-23 2022-12-29 Firmenich Sa Compositions modifiant l'arôme contenant de la lactase

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