JP4861584B2 - イオンチャネルの電気生理的性質を測定及び/または監視するための基体及び方法 - Google Patents
イオンチャネルの電気生理的性質を測定及び/または監視するための基体及び方法 Download PDFInfo
- Publication number
- JP4861584B2 JP4861584B2 JP2001528683A JP2001528683A JP4861584B2 JP 4861584 B2 JP4861584 B2 JP 4861584B2 JP 2001528683 A JP2001528683 A JP 2001528683A JP 2001528683 A JP2001528683 A JP 2001528683A JP 4861584 B2 JP4861584 B2 JP 4861584B2
- Authority
- JP
- Japan
- Prior art keywords
- substrate
- electrode
- site
- ion channel
- electrodes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK199901407 | 1999-10-01 | ||
| DKPA199901407 | 1999-10-01 | ||
| DKPA199901407 | 1999-10-01 | ||
| PCT/DK2000/000548 WO2001025769A2 (en) | 1999-10-01 | 2000-10-02 | A substrate and a method for determining and/or monitoring electrophysiological properties of ion channels |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2003511668A JP2003511668A (ja) | 2003-03-25 |
| JP2003511668A5 JP2003511668A5 (https=) | 2007-02-01 |
| JP4861584B2 true JP4861584B2 (ja) | 2012-01-25 |
Family
ID=8104506
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001528683A Expired - Lifetime JP4861584B2 (ja) | 1999-10-01 | 2000-10-02 | イオンチャネルの電気生理的性質を測定及び/または監視するための基体及び方法 |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP1221046B1 (https=) |
| JP (1) | JP4861584B2 (https=) |
| CN (1) | CN1204404C (https=) |
| AT (1) | ATE317545T1 (https=) |
| AU (1) | AU780157B2 (https=) |
| CA (1) | CA2385482C (https=) |
| DE (1) | DE60025929T2 (https=) |
| DK (1) | DK1221046T3 (https=) |
| IL (1) | IL148650A0 (https=) |
| NZ (1) | NZ518057A (https=) |
| WO (1) | WO2001025769A2 (https=) |
Families Citing this family (91)
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|---|---|---|---|---|
| US20020144905A1 (en) | 1997-12-17 | 2002-10-10 | Christian Schmidt | Sample positioning and analysis system |
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| DE19948473A1 (de) * | 1999-10-08 | 2001-04-12 | Nmi Univ Tuebingen | Verfahren und Vorrichtung zum Messen an in einer flüssigen Umgebung befindlichen Zellen |
| FR2802078B1 (fr) * | 1999-12-14 | 2003-10-03 | Univ Joseph Fourier | Microelectrode support de cellules a membrane excitable |
| JP2004514417A (ja) * | 2000-07-07 | 2004-05-20 | ブリストル−マイヤーズ スクイブ カンパニー | 薬品発見用化合物の高速処理スクリーニングに適した電気生理学的配置 |
| US7067046B2 (en) | 2000-08-04 | 2006-06-27 | Essen Instruments, Inc. | System for rapid chemical activation in high-throughput electrophysiological measurements |
| US7270730B2 (en) | 2000-08-04 | 2007-09-18 | Essen Instruments, Inc. | High-throughput electrophysiological measurement system |
| WO2002024862A2 (en) * | 2000-09-19 | 2002-03-28 | Cytion S.A. | Sample positioning and analysis system |
| DK1322955T3 (da) * | 2000-10-02 | 2012-09-10 | Sophion Bioscience As | System til elektrofysiologiske målinger |
| US6932893B2 (en) | 2000-10-02 | 2005-08-23 | Sophion Bioscience A/S | System for electrophysiological measurements |
| US6776896B1 (en) | 2000-10-11 | 2004-08-17 | Axon Instruments, Inc. | Method of positioning cells for electrophysiological testing |
| WO2002055653A1 (en) * | 2001-01-09 | 2002-07-18 | Matsushita Electric Industrial Co., Ltd. | Device for measuring extracellular potential, method of measuring extracellular potential by using the same and apparatus for quickly screening drug provided therewith |
| US7968305B2 (en) | 2001-03-24 | 2011-06-28 | Aviva Biosciences Corporation | Biochips including ion transport detecting structures and methods of use |
| US20060029955A1 (en) | 2001-03-24 | 2006-02-09 | Antonio Guia | High-density ion transport measurement biochip devices and methods |
| US6899800B2 (en) | 2001-06-20 | 2005-05-31 | Axon Instruments, Inc. | Polymeric electrode for electrophysiological testing |
| CN1529814A (zh) * | 2001-06-20 | 2004-09-15 | 索菲昂生物科学有限公司 | 一种用于确定和/或监控离子通道电生理学性质的装置和方法 |
| JP2003043009A (ja) | 2001-07-30 | 2003-02-13 | Matsushita Electric Ind Co Ltd | 生体試料の発する物理化学的変化を検出するデバイスおよび装置 |
| DE10142393C1 (de) * | 2001-08-30 | 2003-01-23 | Fraunhofer Ges Forschung | Vorrichtung und Verfahren zur Erfassung bioelektrischer Signale aus elektrophysiologisch aktiven Bereichen in Sphäroiden |
| DE10202887B4 (de) * | 2002-01-25 | 2004-05-06 | Advalytix Ag | Zellanalyseverfahren |
| JP2005517180A (ja) * | 2002-02-05 | 2005-06-09 | ザ ユニヴァーシティー コート オブ ザ ユニヴァーシティー オブ グラスゴー | 細胞検定を実施するための装置 |
| EP1476536B1 (en) | 2002-02-12 | 2011-07-20 | Cellectricon Ab | Systems and methods for rapidly changing the solution environment around sensors |
| US7470518B2 (en) | 2002-02-12 | 2008-12-30 | Cellectricon Ab | Systems and method for rapidly changing the solution environment around sensors |
| US7390650B2 (en) | 2002-08-21 | 2008-06-24 | Cellectricon Ab | System and method for obtaining and maintaining high-resistance seals in patch clamp recordings |
| GB0207845D0 (en) * | 2002-04-04 | 2002-05-15 | Lgc Ltd | Apparatus, methods and means for biochemical analysis |
| WO2003089564A1 (en) * | 2002-04-17 | 2003-10-30 | Sophion Bioscience A/S | Substrate and method for measuring the electrophysiological properties of cell membranes |
| GB2398635A (en) | 2003-02-21 | 2004-08-25 | Sophion Bioscience As | A substrate providing a cell gigaseal for a patch clamp |
| EP1501924A4 (en) * | 2002-05-04 | 2006-05-24 | Aviva Biosciences Corp | DEVICE WITH STRUCTURES OF PROTECTIVE ION TRANSPORT AND USE METHOD |
| AU2003234787A1 (en) * | 2002-05-13 | 2003-11-11 | Matsushita Electric Industrial Co., Ltd. | Instrument and method for measuring action signal of biological sample |
| ES2208082B1 (es) * | 2002-05-29 | 2005-09-16 | Jose Luis Bardasano Rubio | Dispositivo para la medida del potencial de la accion transmembrana en preparaciones celulares bajo la accion de campos electromagneticos de frecuencia e intensidad variables. |
| WO2007072790A1 (ja) | 2005-12-20 | 2007-06-28 | Matsushita Electric Industrial Co., Ltd. | 細胞電気生理センサ |
| JP3861831B2 (ja) * | 2003-03-07 | 2006-12-27 | 松下電器産業株式会社 | 細胞外電位測定デバイスおよびその製造方法 |
| WO2006132116A1 (ja) | 2005-06-07 | 2006-12-14 | Matsushita Electric Industrial Co., Ltd. | 細胞電気生理測定デバイスおよびその製造方法 |
| EP1411351A4 (en) * | 2002-06-05 | 2010-07-07 | Panasonic Corp | DEVICE FOR MEASURING THE EXTRACELLULAR POTENTIAL AND METHOD FOR PRODUCING THE DEVICE |
| JP3925439B2 (ja) * | 2003-03-07 | 2007-06-06 | 松下電器産業株式会社 | 細胞外電位測定デバイスおよびその製造方法 |
| US8202439B2 (en) | 2002-06-05 | 2012-06-19 | Panasonic Corporation | Diaphragm and device for measuring cellular potential using the same, manufacturing method of the diaphragm |
| JP3945338B2 (ja) * | 2002-08-01 | 2007-07-18 | 松下電器産業株式会社 | 細胞外電位測定デバイスおよびその製造方法 |
| JP4552423B2 (ja) * | 2003-11-21 | 2010-09-29 | パナソニック株式会社 | 細胞外電位測定デバイスおよびこれを用いた細胞外電位の測定方法 |
| JP3945317B2 (ja) * | 2002-06-05 | 2007-07-18 | 松下電器産業株式会社 | 細胞外電位測定デバイスおよびその製造方法 |
| WO2004079354A1 (ja) * | 2003-03-07 | 2004-09-16 | Matsushita Electric Industrial Co., Ltd. | 細胞外電位測定デバイスおよびその製造方法 |
| WO2003104455A1 (en) * | 2002-06-07 | 2003-12-18 | Sophion Bioscience A/S | Screening methods |
| US7732127B2 (en) | 2002-12-20 | 2010-06-08 | Acea Biosciences, Inc. | Dynamic monitoring of cell adhesion and spreading using the RT-CES system |
| US7192752B2 (en) | 2002-12-20 | 2007-03-20 | Acea Biosciences | Real time electronic cell sensing systems and applications for cell-based assays |
| US8206903B2 (en) | 2002-12-20 | 2012-06-26 | Acea Biosciences | Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses |
| US7468255B2 (en) | 2002-12-20 | 2008-12-23 | Acea Biosciences | Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology |
| WO2004010102A2 (en) * | 2002-07-20 | 2004-01-29 | Acea Biosciences, Inc. | Impedance based apparatuses and methods for analyzing cells and particles |
| US8263375B2 (en) | 2002-12-20 | 2012-09-11 | Acea Biosciences | Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology |
| US7470533B2 (en) | 2002-12-20 | 2008-12-30 | Acea Biosciences | Impedance based devices and methods for use in assays |
| US7560269B2 (en) | 2002-12-20 | 2009-07-14 | Acea Biosciences, Inc. | Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays |
| US7810380B2 (en) | 2003-03-25 | 2010-10-12 | Tearlab Research, Inc. | Systems and methods for collecting tear film and measuring tear film osmolarity |
| EP1801586B1 (en) * | 2002-08-21 | 2010-10-13 | Cellectricon Ab | System for obtaining and maintaining high-resistance seals in patch clamp recordings |
| FR2844052B1 (fr) | 2002-08-28 | 2005-07-01 | Commissariat Energie Atomique | Dispositif de mesure de l'activite electrique d'elements biologiques et ses applications |
| WO2004036202A1 (en) | 2002-10-16 | 2004-04-29 | Cellectricon Ab | Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells |
| US9612234B2 (en) | 2008-05-05 | 2017-04-04 | Acea Biosciences, Inc. | Data analysis of impedance-based cardiomyocyte-beating signals as detected on real-time cell analysis (RTCA) cardio instruments |
| US10539523B2 (en) | 2002-12-20 | 2020-01-21 | Acea Biosciences, Inc. | System and method for monitoring cardiomyocyte beating, viability, morphology, and electrophysiological properties |
| US10551371B2 (en) | 2003-11-10 | 2020-02-04 | Acea Biosciences, Inc. | System and method for monitoring cardiomyocyte beating, viability and morphology and for screening for pharmacological agents which may induce cardiotoxicity or modulate cardiomyocyte function |
| US10215748B2 (en) | 2002-12-20 | 2019-02-26 | Acea Biosciences, Inc. | Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways |
| US11346797B2 (en) | 2002-12-20 | 2022-05-31 | Agilent Technologies, Inc. | System and method for monitoring cardiomyocyte beating, viability, morphology and electrophysiological properties |
| GB0303920D0 (en) | 2003-02-21 | 2003-03-26 | Sophion Bioscience As | Capillary stop |
| EP1634075B1 (en) * | 2003-06-10 | 2015-01-14 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Electronic device for communication with living cells |
| CA2556219A1 (en) * | 2004-02-09 | 2005-08-25 | Acea Biosciences, Inc. | Methods for assaying cells using cell-substrate impedance monitoring |
| FR2866958B1 (fr) | 2004-02-26 | 2006-08-04 | Commissariat Energie Atomique | Procede et dispositif de controle du positionnement d'un element biologique sur un support |
| JP4897681B2 (ja) * | 2004-07-23 | 2012-03-14 | エレクトロニック・バイオサイエンシーズ・エルエルシー | イオン・チャネルを通過する時間的に変化する電流を検出するための方法及び装置 |
| EP1783202B1 (en) | 2004-08-25 | 2013-10-02 | Panasonic Corporation | Probe for measuring electric potential of cell |
| JP2006184207A (ja) * | 2004-12-28 | 2006-07-13 | Matsushita Electric Ind Co Ltd | 細胞膜の特性および状態の少なくとも一方の電気的測定方法および電気的測定装置 |
| US20060216203A1 (en) * | 2005-03-28 | 2006-09-28 | Mds Sciex (Us) A Division Of Mds Pharma Services (Us) Inc. | Multiwell sample plate with integrated impedance electrodes and connection scheme |
| JP4661539B2 (ja) * | 2005-11-11 | 2011-03-30 | パナソニック株式会社 | 細胞電気生理センサおよびその製造方法 |
| US7608417B2 (en) * | 2005-11-14 | 2009-10-27 | Panasonic Corporation | Cell electro-physiological sensor and method of manufacturing the same |
| JP2009156572A (ja) * | 2006-04-06 | 2009-07-16 | National Institutes Of Natural Sciences | イオンチャンネルタンパク質バイオセンサー |
| US8071363B2 (en) | 2006-05-25 | 2011-12-06 | Panasonic Corporation | Chip for cell electrophysiological sensor, cell electrophysiological sensor using the same, and manufacturing method of chip for cell electrophysiological sensor |
| US8445263B2 (en) | 2006-07-06 | 2013-05-21 | Panasonic Corporation | Device for cellular electrophysiology sensor, cellular electrophysiology sensor using the device, and method for manufacturing the cellular electrophysiology sensor device |
| US8041515B2 (en) | 2006-09-20 | 2011-10-18 | Acea Biosciences, Inc. | Use of impedance-based cytological profiling to classify cellular response profiles upon exposure to biologically active agents |
| WO2008137716A2 (en) * | 2007-05-04 | 2008-11-13 | Tecella, Llc | Subsystems and methods for use in patch clamp systems |
| US8314466B2 (en) | 2007-09-11 | 2012-11-20 | Panasonic Corporation | Silicon structure, method for manufacturing the same, and sensor chip |
| CA2723223C (en) | 2008-05-05 | 2017-06-06 | Acea Biosciences, Inc. | Label-free monitoring of excitation-contraction coupling and excitable cells using impedance based systems with millisecond time resolution |
| CN103068486B (zh) * | 2010-07-09 | 2015-09-23 | 索菲昂生物科学有限公司 | 用于微流体分析系统中的芯片组件 |
| JP5575623B2 (ja) * | 2010-12-03 | 2014-08-20 | マルボシ酢株式会社 | 誘電特性測定用耐圧容器センサーに用いる絶縁体 |
| JP6037717B2 (ja) | 2011-12-20 | 2016-12-07 | 国立研究開発法人科学技術振興機構 | プレーナーパッチクランプ装置、該装置用電極部及び細胞イオンチャンネル電流計測方法 |
| CA2860501C (en) * | 2012-01-09 | 2021-05-18 | Sophion Bioscience A/S | Improved patch area cell adhesion |
| DE102013007295B4 (de) * | 2013-04-26 | 2015-06-25 | Universität Rostock | Elektrophysiologische Messanordnung und elektrophysiologisches Messverfahren |
| SG11201601487XA (en) | 2013-08-30 | 2016-04-28 | Japan Science & Tech Agency | Planar patch clamp device and planar patch clamp system |
| CN106716116B (zh) | 2014-09-23 | 2021-04-27 | 蒂尔实验室研究有限公司 | 用于微流体泪液收集和感兴趣的分析物的横向流动分析的集成的系统和方法 |
| US12066428B2 (en) | 2015-11-20 | 2024-08-20 | Agilent Technologies, Inc. | Cell-substrate impedance monitoring of cancer cells |
| WO2017175879A1 (ja) * | 2016-04-05 | 2017-10-12 | シャープ株式会社 | センサ装置、検出方法、及びセンサユニット |
| JP6676486B2 (ja) * | 2016-04-05 | 2020-04-08 | シャープ株式会社 | 検出方法 |
| CN110582569B (zh) | 2017-03-03 | 2024-04-02 | 安捷伦科技有限公司 | 用于iPSC和ESC衍生的心肌细胞的功能成熟的方法和系统 |
| TWI695166B (zh) * | 2017-11-03 | 2020-06-01 | 國立臺灣大學 | 一種多離子感測電極陣列試片及其感測裝置 |
| JP7229110B2 (ja) * | 2019-06-25 | 2023-02-27 | 株式会社Screenホールディングス | 細胞電位測定装置 |
| JP7469092B2 (ja) | 2020-03-23 | 2024-04-16 | 株式会社Screenホールディングス | 細胞保持容器 |
| US20210301245A1 (en) | 2020-03-29 | 2021-09-30 | Agilent Technologies, Inc. | Systems and methods for electronically and optically monitoring biological samples |
| EP4460695B1 (en) | 2022-01-07 | 2025-12-24 | Sophion Bioscience A/S | Semi-automated patch-clamp apparatus and a method for carrying out a patch-clamp procedure |
| EP4460694A1 (en) | 2022-01-07 | 2024-11-13 | Sophion Bioscience A/S | Pipette guidance in multiple-well plate patch-clamp |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4062750A (en) * | 1974-12-18 | 1977-12-13 | James Francis Butler | Thin film electrochemical electrode and cell |
| US4225410A (en) * | 1978-12-04 | 1980-09-30 | Technicon Instruments Corporation | Integrated array of electrochemical sensors |
| US4874500A (en) * | 1987-07-15 | 1989-10-17 | Sri International | Microelectrochemical sensor and sensor array |
| US4812221A (en) * | 1987-07-15 | 1989-03-14 | Sri International | Fast response time microsensors for gaseous and vaporous species |
| DE4115414C2 (de) * | 1991-05-10 | 1995-07-06 | Meinhard Prof Dr Knoll | Verfahren zur Herstellung von miniaturisierten Chemo- und Biosensorelementen mit ionenselektiver Membran sowie von Trägern für diese Elemente |
| IL103674A0 (en) * | 1991-11-19 | 1993-04-04 | Houston Advanced Res Center | Method and apparatus for molecule detection |
| US6758961B1 (en) * | 1997-12-17 | 2004-07-06 | Ecole Polytechnique Federale De Lausanne | Positioning and electrophysiological characterization of individual cells and reconstituted membrane systems on microstructured carriers |
-
2000
- 2000-10-02 NZ NZ518057A patent/NZ518057A/en not_active IP Right Cessation
- 2000-10-02 EP EP00962271A patent/EP1221046B1/en not_active Expired - Lifetime
- 2000-10-02 WO PCT/DK2000/000548 patent/WO2001025769A2/en not_active Ceased
- 2000-10-02 JP JP2001528683A patent/JP4861584B2/ja not_active Expired - Lifetime
- 2000-10-02 IL IL14865000A patent/IL148650A0/xx unknown
- 2000-10-02 CA CA2385482A patent/CA2385482C/en not_active Expired - Fee Related
- 2000-10-02 DE DE60025929T patent/DE60025929T2/de not_active Expired - Lifetime
- 2000-10-02 AT AT00962271T patent/ATE317545T1/de not_active IP Right Cessation
- 2000-10-02 CN CNB008136157A patent/CN1204404C/zh not_active Expired - Fee Related
- 2000-10-02 AU AU74065/00A patent/AU780157B2/en not_active Ceased
- 2000-10-02 DK DK00962271T patent/DK1221046T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| IL148650A0 (en) | 2002-09-12 |
| WO2001025769A2 (en) | 2001-04-12 |
| DE60025929T2 (de) | 2006-11-09 |
| DK1221046T3 (da) | 2006-06-12 |
| AU780157B2 (en) | 2005-03-03 |
| NZ518057A (en) | 2004-01-30 |
| DE60025929D1 (de) | 2006-04-20 |
| CN1377464A (zh) | 2002-10-30 |
| JP2003511668A (ja) | 2003-03-25 |
| ATE317545T1 (de) | 2006-02-15 |
| CA2385482C (en) | 2011-01-18 |
| WO2001025769A3 (en) | 2001-08-30 |
| EP1221046B1 (en) | 2006-02-08 |
| AU7406500A (en) | 2001-05-10 |
| EP1221046A2 (en) | 2002-07-10 |
| CN1204404C (zh) | 2005-06-01 |
| CA2385482A1 (en) | 2001-04-12 |
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