JP4832694B2 - 増加した熱安定性を有するｒｎａポリメラーゼ変異体 - Google Patents

増加した熱安定性を有するｒｎａポリメラーゼ変異体 Download PDF

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JP4832694B2
JP4832694B2 JP2001565862A JP2001565862A JP4832694B2 JP 4832694 B2 JP4832694 B2 JP 4832694B2 JP 2001565862 A JP2001565862 A JP 2001565862A JP 2001565862 A JP2001565862 A JP 2001565862A JP 4832694 B2 JP4832694 B2 JP 4832694B2
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rna polymerase
mutation
enzyme
rna
gene
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JP2003525627A5 (enExample
JP2003525627A (ja
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明生 杉山
西矢　　芳昭
川上　　文清
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アクゾ・ノベル・エヌ・ベー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Heat Sensitive Colour Forming Recording (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
JP2001565862A 2000-03-07 2001-03-01 増加した熱安定性を有するｒｎａポリメラーゼ変異体 Expired - Lifetime JP4832694B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00200787 2000-03-07
EP00200787.0 2000-03-07
PCT/EP2001/002327 WO2001066705A1 (en) 2000-03-07 2001-03-01 Rna polymerase mutants with increased thermostability

Publications (3)

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JP2003525627A JP2003525627A (ja) 2003-09-02
JP2003525627A5 JP2003525627A5 (enExample) 2008-04-17
JP4832694B2 true JP4832694B2 (ja) 2011-12-07

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JP2001565862A Expired - Lifetime JP4832694B2 (ja) 2000-03-07 2001-03-01 増加した熱安定性を有するｒｎａポリメラーゼ変異体

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US (1) US7507567B2 (enExample)
EP (1) EP1261696B1 (enExample)
JP (1) JP4832694B2 (enExample)
AT (1) ATE294855T1 (enExample)
AU (1) AU2001237425A1 (enExample)
CA (1) CA2400049C (enExample)
DE (1) DE60110564T2 (enExample)
ES (1) ES2242733T3 (enExample)
WO (1) WO2001066705A1 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009136153A (ja) * 2007-12-03 2009-06-25 Tosoh Corp 変異型ｒｎａポリメラーゼ
WO2009113718A1 (ja) * 2008-03-11 2009-09-17 東ソー株式会社 機能改善したｒｎａポリメラーゼ変異体
WO2010016621A1 (ja) 2008-08-08 2010-02-11 東ソー株式会社 機能改善したｒｎａポリメラーゼ変異体
US9193959B2 (en) 2010-04-16 2015-11-24 Roche Diagnostics Operations, Inc. T7 RNA polymerase variants with enhanced thermostability
ES2839424T3 (es) 2010-08-31 2021-07-05 Greenlight Biosciences Inc Métodos para control de flujo en vías metabólicas mediante manipulación de proteasas
US9062292B2 (en) 2010-09-13 2015-06-23 Enzo Life Sciences Inc. Mutant T7 polymerases
FR2981088B1 (fr) 2011-10-06 2013-11-29 Biomerieux Sa Arn polymerases mutees
US9045740B2 (en) * 2012-02-24 2015-06-02 University Of Massachusetts Modified T7-related RNA polymerases and methods of use thereof
SG11201600898YA (en) 2013-08-05 2016-03-30 Greenlight Biosciences Inc Engineered proteins with a protease cleavage site
WO2015024017A2 (en) * 2013-08-16 2015-02-19 President And Fellows Of Harvard College Rna polymerase, methods of purification and methods of use
MX2016012206A (es) 2014-03-20 2017-04-06 Univ Texas Variantes de t7 rna polimerasa con rango de substrato expandido y rendimiento de transcripcion mejorado.
EP3277808A4 (en) 2015-03-30 2019-05-22 Greenlight Biosciences, Inc. ACELLULAR PRODUCTION OF RIBONUCLEIC ACID
US20190017100A1 (en) * 2015-07-01 2019-01-17 Curevac Ag Method for analysis of an rna molecule
CN116144626A (zh) 2016-01-13 2023-05-23 新英格兰生物实验室公司 T7 rna聚合酶的热稳定变体
RU2018138975A (ru) 2016-04-06 2020-05-12 Гринлайт Байосайенсис, Инк. Бесклеточная продукция рибонуклеиновой кислоты
US10034951B1 (en) * 2017-06-21 2018-07-31 New England Biolabs, Inc. Use of thermostable RNA polymerases to produce RNAs having reduced immunogenicity
JP7588391B2 (ja) 2017-06-30 2024-11-22 コデクシス， インコーポレイテッド Ｔ７ ｒｎａポリメラーゼバリアント
AU2018292104B2 (en) 2017-06-30 2025-03-27 Codexis, Inc. T7 RNA polymerase variants
WO2019075167A1 (en) 2017-10-11 2019-04-18 Greenlight Biosciences, Inc. METHODS AND COMPOSITIONS FOR THE PRODUCTION OF NUCLEOSIDE TRIPHOSPHATE AND RIBONUCLEIC ACID
WO2019199807A1 (en) * 2018-04-10 2019-10-17 Greenlight Biosciences, Inc. T7 rna polymerase variants
EP3976770A1 (en) * 2019-05-24 2022-04-06 Primordial Genetics Inc. Methods and compositions for manufacturing polynucleotides
CN112980811B (zh) * 2021-03-06 2022-02-25 苏州瀚源新酶生物科技有限公司 Rna聚合酶突变体及其应用、重组载体及其制备方法和应用、重组工程菌及其应用
EP4108770A1 (en) 2021-06-24 2022-12-28 Aptamimetics GmbH Age-selex method for aptamer identification and use
WO2025127053A1 (ja) * 2023-12-12 2025-06-19 タカラバイオ株式会社 Ｒｎａポリメラーゼ変異体とｒｎａ合成

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1175867A (ja) * 1997-07-07 1999-03-23 Rikagaku Kenkyusho Ｒｎａポリメラーゼ
JP2001054387A (ja) * 1999-08-17 2001-02-27 Toyobo Co Ltd 改良されたｒｎａポリメラーゼ
JP2002532095A (ja) * 1998-12-11 2002-10-02 アクゾ・ノベル・エヌ・ベー 安定性を増大させたｒｎａポリメラーゼ変異体

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4952496A (en) * 1984-03-30 1990-08-28 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5385834A (en) * 1993-08-13 1995-01-31 Georgia Tech Research Corporation Mutant T7 RNA polymerase GP1(lys222) exhibiting altered promoter recognition
FR2761695B1 (fr) * 1997-04-04 1999-09-24 Bio Merieux Arn polymerase fonctionnant preferentiellement sur matrice d'arn, sa preparation et son utilisation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1175867A (ja) * 1997-07-07 1999-03-23 Rikagaku Kenkyusho Ｒｎａポリメラーゼ
JP2002532095A (ja) * 1998-12-11 2002-10-02 アクゾ・ノベル・エヌ・ベー 安定性を増大させたｒｎａポリメラーゼ変異体
JP2001054387A (ja) * 1999-08-17 2001-02-27 Toyobo Co Ltd 改良されたｒｎａポリメラーゼ

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Publication number Publication date
EP1261696B1 (en) 2005-05-04
US20030175738A1 (en) 2003-09-18
US7507567B2 (en) 2009-03-24
ES2242733T3 (es) 2005-11-16
EP1261696A1 (en) 2002-12-04
ATE294855T1 (de) 2005-05-15
CA2400049C (en) 2010-10-05
DE60110564T2 (de) 2006-01-19
AU2001237425A1 (en) 2001-09-17
DE60110564D1 (en) 2005-06-09
CA2400049A1 (en) 2001-09-13
WO2001066705A1 (en) 2001-09-13
JP2003525627A (ja) 2003-09-02

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