CA2400049C - Rna polymerase mutants with increased thermostability - Google Patents

Rna polymerase mutants with increased thermostability Download PDF

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Publication number
CA2400049C
CA2400049C CA2400049A CA2400049A CA2400049C CA 2400049 C CA2400049 C CA 2400049C CA 2400049 A CA2400049 A CA 2400049A CA 2400049 A CA2400049 A CA 2400049A CA 2400049 C CA2400049 C CA 2400049C
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CA
Canada
Prior art keywords
rna polymerase
rna
mutated
gene
mutant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2400049A
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English (en)
French (fr)
Other versions
CA2400049A1 (en
Inventor
Akio Sugiyama
Yoshiaki Nishiya
Bunsei Kawakami
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Biomerieux BV
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Biomerieux BV
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Publication date
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Publication of CA2400049A1 publication Critical patent/CA2400049A1/en
Application granted granted Critical
Publication of CA2400049C publication Critical patent/CA2400049C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Heat Sensitive Colour Forming Recording (AREA)
CA2400049A 2000-03-07 2001-03-01 Rna polymerase mutants with increased thermostability Expired - Lifetime CA2400049C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00200787.0 2000-03-07
EP00200787 2000-03-07
PCT/EP2001/002327 WO2001066705A1 (en) 2000-03-07 2001-03-01 Rna polymerase mutants with increased thermostability

Publications (2)

Publication Number Publication Date
CA2400049A1 CA2400049A1 (en) 2001-09-13
CA2400049C true CA2400049C (en) 2010-10-05

Family

ID=8171152

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2400049A Expired - Lifetime CA2400049C (en) 2000-03-07 2001-03-01 Rna polymerase mutants with increased thermostability

Country Status (9)

Country Link
US (1) US7507567B2 (enExample)
EP (1) EP1261696B1 (enExample)
JP (1) JP4832694B2 (enExample)
AT (1) ATE294855T1 (enExample)
AU (1) AU2001237425A1 (enExample)
CA (1) CA2400049C (enExample)
DE (1) DE60110564T2 (enExample)
ES (1) ES2242733T3 (enExample)
WO (1) WO2001066705A1 (enExample)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009136153A (ja) * 2007-12-03 2009-06-25 Tosoh Corp 変異型ｒｎａポリメラーゼ
WO2009113718A1 (ja) * 2008-03-11 2009-09-17 東ソー株式会社 機能改善したｒｎａポリメラーゼ変異体
US8551752B2 (en) 2008-08-08 2013-10-08 Tosoh Corporation RNA polymerase mutant with improved functions
US9193959B2 (en) 2010-04-16 2015-11-24 Roche Diagnostics Operations, Inc. T7 RNA polymerase variants with enhanced thermostability
US8916358B2 (en) 2010-08-31 2014-12-23 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
US9062292B2 (en) 2010-09-13 2015-06-23 Enzo Life Sciences Inc. Mutant T7 polymerases
FR2981088B1 (fr) 2011-10-06 2013-11-29 Biomerieux Sa Arn polymerases mutees
US9045740B2 (en) * 2012-02-24 2015-06-02 University Of Massachusetts Modified T7-related RNA polymerases and methods of use thereof
WO2015021058A2 (en) 2013-08-05 2015-02-12 Greenlight Biosciences, Inc. Engineered proteins with a protease cleavage site
WO2015024017A2 (en) * 2013-08-16 2015-02-19 President And Fellows Of Harvard College Rna polymerase, methods of purification and methods of use
AU2015231031A1 (en) * 2014-03-20 2016-09-22 The Board Of Regents Of The University Of Texas System T7 RNA polymerase variants with expanded substrate range and enhanced transcriptional yield
RU2017137788A (ru) 2015-03-30 2019-04-30 Гринлайт Байосайенсис, Инк. Бесклеточное получение рибонуклеиновой кислоты
EP4239080A3 (en) * 2015-07-01 2023-11-01 CureVac Manufacturing GmbH Method for analysis of an rna molecule
EP3402881B1 (en) * 2016-01-13 2021-04-28 New England Biolabs, Inc. Thermostable variants of t7 rna polymerase
SG11201808721YA (en) 2016-04-06 2018-11-29 Greenlight Biosciences Inc Cell-free production of ribonucleic acid
US10034951B1 (en) 2017-06-21 2018-07-31 New England Biolabs, Inc. Use of thermostable RNA polymerases to produce RNAs having reduced immunogenicity
JP2020530267A (ja) 2017-06-30 2020-10-22 コデクシス， インコーポレイテッド Ｔ７ ｒｎａポリメラーゼバリアント
KR102773542B1 (ko) 2017-06-30 2025-02-25 코덱시스, 인코포레이티드 T7 rna 폴리머라제 변이체
WO2019075167A1 (en) 2017-10-11 2019-04-18 Greenlight Biosciences, Inc. METHODS AND COMPOSITIONS FOR THE PRODUCTION OF NUCLEOSIDE TRIPHOSPHATE AND RIBONUCLEIC ACID
US20210180034A1 (en) * 2018-04-10 2021-06-17 Greenlight Biosciences, Inc. T7 rna polymerase variants
WO2020243026A1 (en) * 2019-05-24 2020-12-03 Primordial Genetics Inc. Methods and compositions for manufacturing polynucleotides
CN112980811B (zh) * 2021-03-06 2022-02-25 苏州瀚源新酶生物科技有限公司 Rna聚合酶突变体及其应用、重组载体及其制备方法和应用、重组工程菌及其应用
EP4108770A1 (en) 2021-06-24 2022-12-28 Aptamimetics GmbH Age-selex method for aptamer identification and use
WO2025127053A1 (ja) 2023-12-12 2025-06-19 タカラバイオ株式会社 Ｒｎａポリメラーゼ変異体とｒｎａ合成
WO2026089040A1 (ja) * 2024-10-25 2026-04-30 タカラバイオ株式会社 Ｒｎａポリメラーゼ変異体
US20260185136A1 (en) 2024-12-27 2026-07-02 Eisai R&D Management Co., Ltd. 5'-cap modifications of rna

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4952496A (en) * 1984-03-30 1990-08-28 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5385834A (en) * 1993-08-13 1995-01-31 Georgia Tech Research Corporation Mutant T7 RNA polymerase GP1(lys222) exhibiting altered promoter recognition
FR2761695B1 (fr) * 1997-04-04 1999-09-24 Bio Merieux Arn polymerase fonctionnant preferentiellement sur matrice d'arn, sa preparation et son utilisation
JP3172710B2 (ja) * 1997-07-07 2001-06-04 理化学研究所 Ｒｎａポリメラーゼ
JP4485066B2 (ja) * 1998-12-11 2010-06-16 アクゾ・ノベル・エヌ・ベー 安定性を増大させたｒｎａポリメラーゼ変異体
JP4399684B2 (ja) * 1999-08-17 2010-01-20 東洋紡績株式会社 改良されたｒｎａポリメラーゼ

Also Published As

Publication number Publication date
DE60110564T2 (de) 2006-01-19
CA2400049A1 (en) 2001-09-13
ATE294855T1 (de) 2005-05-15
JP2003525627A (ja) 2003-09-02
DE60110564D1 (en) 2005-06-09
JP4832694B2 (ja) 2011-12-07
US20030175738A1 (en) 2003-09-18
AU2001237425A1 (en) 2001-09-17
WO2001066705A1 (en) 2001-09-13
US7507567B2 (en) 2009-03-24
EP1261696B1 (en) 2005-05-04
EP1261696A1 (en) 2002-12-04
ES2242733T3 (es) 2005-11-16

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Effective date: 20210301