JP4748542B2 - フローサイトメトリーによる生体分子の定量システム、及び定量方法 - Google Patents

フローサイトメトリーによる生体分子の定量システム、及び定量方法 Download PDF

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Publication number
JP4748542B2
JP4748542B2 JP2008501743A JP2008501743A JP4748542B2 JP 4748542 B2 JP4748542 B2 JP 4748542B2 JP 2008501743 A JP2008501743 A JP 2008501743A JP 2008501743 A JP2008501743 A JP 2008501743A JP 4748542 B2 JP4748542 B2 JP 4748542B2
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Prior art keywords
silica particles
fluorescent
types
fluorescence
fluorescent silica
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Expired - Fee Related
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Japanese (ja)
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JPWO2007097377A1 (ja
Inventor
英樹 會澤
典雄 大久保
教泰 中村
弘一 三好
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Furukawa Electric Co Ltd
University of Tokushima NUC
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Furukawa Electric Co Ltd
University of Tokushima NUC
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/552—Glass or silica
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/13—Tracers or tags
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Inorganic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2008501743A 2006-02-24 2007-02-21 フローサイトメトリーによる生体分子の定量システム、及び定量方法 Expired - Fee Related JP4748542B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008501743A JP4748542B2 (ja) 2006-02-24 2007-02-21 フローサイトメトリーによる生体分子の定量システム、及び定量方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2006049303 2006-02-24
JP2006049303 2006-02-24
JP2008501743A JP4748542B2 (ja) 2006-02-24 2007-02-21 フローサイトメトリーによる生体分子の定量システム、及び定量方法
PCT/JP2007/053227 WO2007097377A1 (ja) 2006-02-24 2007-02-21 フローサイトメトリーによる生体分子の定量システム、その定量方法、細胞の検出・分取システム、その検出・分取方法、それらに用いる蛍光シリカ粒子、及び複数個の蛍光シリカ粒子を組み合わせたキット

Related Child Applications (2)

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JP2008177413A Division JP4572274B2 (ja) 2006-02-24 2008-07-07 標的生体分子標識用もしくは標的細胞標識用の蛍光シリカ粒子組成物、及びこれを用いたフローサイトメトリー用マルチプレックシングキット
JP2008177409A Division JP4716337B2 (ja) 2006-02-24 2008-07-07 フローサイトメトリーによる細胞の検出・分取システム、及び検出・分取方法

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JPWO2007097377A1 JPWO2007097377A1 (ja) 2009-07-16
JP4748542B2 true JP4748542B2 (ja) 2011-08-17

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JP2008501743A Expired - Fee Related JP4748542B2 (ja) 2006-02-24 2007-02-21 フローサイトメトリーによる生体分子の定量システム、及び定量方法
JP2008177409A Active JP4716337B2 (ja) 2006-02-24 2008-07-07 フローサイトメトリーによる細胞の検出・分取システム、及び検出・分取方法
JP2008177413A Expired - Fee Related JP4572274B2 (ja) 2006-02-24 2008-07-07 標的生体分子標識用もしくは標的細胞標識用の蛍光シリカ粒子組成物、及びこれを用いたフローサイトメトリー用マルチプレックシングキット

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JP2008177409A Active JP4716337B2 (ja) 2006-02-24 2008-07-07 フローサイトメトリーによる細胞の検出・分取システム、及び検出・分取方法
JP2008177413A Expired - Fee Related JP4572274B2 (ja) 2006-02-24 2008-07-07 標的生体分子標識用もしくは標的細胞標識用の蛍光シリカ粒子組成物、及びこれを用いたフローサイトメトリー用マルチプレックシングキット

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US (1) US8796040B2 (https=)
JP (3) JP4748542B2 (https=)
WO (1) WO2007097377A1 (https=)

Cited By (2)

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WO2014024598A1 (ja) 2012-08-08 2014-02-13 株式会社 日立ハイテクノロジーズ 生体分子検出方法、生体分子検出装置、および分析用デバイス
JP2023082660A (ja) * 2021-12-02 2023-06-14 株式会社デンソー 分析方法

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CA2642277A1 (en) * 2005-04-15 2006-10-19 Kanarev Philipp Michaylovich Heat generator
US9265729B2 (en) * 2007-12-06 2016-02-23 The University Of Tokushima Nanofunctional silica particles and manufacturing method thereof
CA2735543C (en) * 2008-09-02 2017-05-02 Alison Jane Basile Method for detection of antigen-specific antibodies in biological samples
JP2010091553A (ja) * 2008-09-09 2010-04-22 Konica Minolta Holdings Inc 生体分子の検出方法
WO2011065753A2 (ko) * 2009-11-24 2011-06-03 가톨릭대학교 산학협력단 형광 강도의 조절을 이용한 유세포 분석법
KR101251590B1 (ko) * 2009-11-24 2013-04-08 가톨릭대학교 산학협력단 형광 강도의 조절을 이용한 유세포 분석법
EP3779433A3 (en) * 2010-08-30 2021-05-19 Konica Minolta, Inc. Tissue staining method, tissue evaluation method and biosubstance detection method
WO2012142301A2 (en) 2011-04-12 2012-10-18 Quanterix Corporation Methods of determining a treatment protocol for and/or a prognosis of a patients recovery from a brain injury
JP5367915B2 (ja) * 2011-04-26 2013-12-11 古河電気工業株式会社 生体分子を結合させた機能性分子含有シリカナノ粒子の製造方法
JP5579343B1 (ja) 2012-11-28 2014-08-27 古河電気工業株式会社 イムノクロマトグラフィー、これに用いられる検出装置および試薬
WO2014136885A1 (ja) * 2013-03-08 2014-09-12 コニカミノルタ株式会社 組織染色用染色剤、組織染色用染色剤の製造方法および組織染色用染色剤を含む組織染色用キット
US9726605B2 (en) 2013-03-14 2017-08-08 Furukawa Electric Co., Ltd. Fluorescence immuno-chromatography, kit and test strip for the same
EP3035057B1 (en) * 2013-08-12 2018-11-14 Furukawa Electric Co. Ltd. Silica particles having reactive functional group on surface, and method for producing the same
US20190137396A1 (en) * 2016-04-28 2019-05-09 National University Corporation Nagoya University Fluorescent probe, method for detecting fluorescence, and method for using fluorescent probe
FR3051556A1 (fr) * 2016-05-18 2017-11-24 Biocytex Utilisation de marqueurs fluorescents pour la detection de cible biologique dans un echantillon
JP6231709B1 (ja) * 2016-05-31 2017-11-15 シスメックス株式会社 蛍光画像分析装置および分析方法
SG11202011500UA (en) 2018-07-12 2020-12-30 Beckman Coulter Inc Fixable viability dyes and their uses
CN110632051B (zh) * 2019-09-29 2022-11-25 浙江海洋大学 一种用流式细胞仪计数并分选荧光标记微塑料颗粒的方法
US20230241255A1 (en) * 2022-01-31 2023-08-03 The Texas A&M University System Magnetic resonance contrast agents and methods thereof
WO2024034371A1 (ja) * 2022-08-10 2024-02-15 株式会社島津製作所 測定方法
US20250020639A1 (en) * 2023-06-18 2025-01-16 Cytek Biosciences, Inc. Ultra-bright nanoparticle fluorescent dye complexes

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JPH0572204A (ja) * 1991-09-12 1993-03-23 Sumitomo Pharmaceut Co Ltd 高感度特異的抗体測定法
JP2002537564A (ja) * 1999-02-17 2002-11-05 デルタゲン プロテオミクス， インコーポレイテッド 基質−リガンド相互作用スクリーニング方法
JP2005506547A (ja) * 2001-10-26 2005-03-03 ビーティーエフ プロプライアタリー リミティド サイトメーター
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Publication number Priority date Publication date Assignee Title
WO2014024598A1 (ja) 2012-08-08 2014-02-13 株式会社 日立ハイテクノロジーズ 生体分子検出方法、生体分子検出装置、および分析用デバイス
US9759681B2 (en) 2012-08-08 2017-09-12 Hitachi High-Technologies Corporation Biomolecule detection method, biomolecule detection device and analysis device
JP2023082660A (ja) * 2021-12-02 2023-06-14 株式会社デンソー 分析方法

Also Published As

Publication number Publication date
JP2009014729A (ja) 2009-01-22
JP2009014730A (ja) 2009-01-22
US8796040B2 (en) 2014-08-05
JPWO2007097377A1 (ja) 2009-07-16
US20090068639A1 (en) 2009-03-12
WO2007097377A1 (ja) 2007-08-30
JP4572274B2 (ja) 2010-11-04
JP4716337B2 (ja) 2011-07-06

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