JP4739352B2 - 真核細胞をトランスフェクトするための固定化分解性カチオンポリマーを持つ固体表面 - Google Patents

真核細胞をトランスフェクトするための固定化分解性カチオンポリマーを持つ固体表面 Download PDF

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JP4739352B2
JP4739352B2 JP2007546895A JP2007546895A JP4739352B2 JP 4739352 B2 JP4739352 B2 JP 4739352B2 JP 2007546895 A JP2007546895 A JP 2007546895A JP 2007546895 A JP2007546895 A JP 2007546895A JP 4739352 B2 JP4739352 B2 JP 4739352B2
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cell
transfection
cationic
poly
degradable
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JP2008523810A (ja
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康進 田中
カステロ、クリス、ピー．
ユー、レイ
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Nitto Denko Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2007546895A 2004-12-17 2005-12-14 真核細胞をトランスフェクトするための固定化分解性カチオンポリマーを持つ固体表面 Expired - Fee Related JP4739352B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US63734404P 2004-12-17 2004-12-17
US60/637,344 2004-12-17
PCT/US2005/045429 WO2006066001A2 (fr) 2004-12-17 2005-12-14 Surface solide comprenant un polymere cationique degradable immobilise afin de transfecter des cellules eucaryotes

Publications (2)

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JP2008523810A JP2008523810A (ja) 2008-07-10
JP4739352B2 true JP4739352B2 (ja) 2011-08-03

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Country Status (7)

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EP (1) EP1836293A2 (fr)
JP (1) JP4739352B2 (fr)
KR (2) KR101263173B1 (fr)
CN (1) CN101124316A (fr)
AU (1) AU2005316501B2 (fr)
CA (1) CA2590820A1 (fr)
WO (1) WO2006066001A2 (fr)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
EP3718635A1 (fr) 2003-07-31 2020-10-07 Handylab, Inc. Traitement d'échantillons contenant des particules
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
EP1774017B1 (fr) 2004-07-26 2013-05-15 Pfenex Inc. Procede permettant d'ameliorer l'expression d'une proteine par mise au point d'une souche par genie genetique
US7358223B2 (en) 2004-10-04 2008-04-15 Nitto Denko Corporation Biodegradable cationic polymers
US9572886B2 (en) 2005-12-22 2017-02-21 Nitto Denko Corporation Agent for treating myelofibrosis
EP2001990B1 (fr) 2006-03-24 2016-06-29 Handylab, Inc. Système intégré de traitement d'échantillons microfluides et son procédé d'utilisation
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
WO2008061165A2 (fr) 2006-11-14 2008-05-22 Handylab, Inc. Cartouche microfluidique et son procédé de fabrication
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
WO2008134461A2 (fr) 2007-04-27 2008-11-06 Dow Global Technologies, Inc. Procédé pour rapidement cribler des hôtes microbiens et identifier certaines souches ayant un rendement et/ou une qualité d'expression des protéines hétérologues améliorés
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
ES2648798T3 (es) * 2007-07-13 2018-01-08 Handylab, Inc. Materiales de captura de polinucleótidos y métodos de utilización de los mismos
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
BR112013026451B1 (pt) 2011-04-15 2021-02-09 Becton, Dickinson And Company sistema e método para realizar ensaios de diagnóstico molecular em várias amostras em paralelo e simultaneamente amplificação em tempo real em pluralidade de câmaras de reação de amplificação
JP6117217B2 (ja) 2011-09-30 2017-04-19 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ ユニット化された試薬ストリップ
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
EP2773892B1 (fr) 2011-11-04 2020-10-07 Handylab, Inc. Dispositif de préparation d'échantillons de polynucléotides
BR112014018995B1 (pt) 2012-02-03 2021-01-19 Becton, Dickson And Company sistemas para executar ensaio automatizado
CN102827756B (zh) * 2012-06-25 2014-04-09 华南农业大学 应用于大豆下胚轴外植体转化受体系统的农杆菌局部侵染方法和装置
CN105579584B (zh) * 2013-06-28 2020-08-28 埃泽瑞斯公司 用于将rna引入细胞的组合物
US20180214572A1 (en) * 2014-11-10 2018-08-02 Ethris Gmbh Induction of osteogenesis by delivering bmp encoding rna
PT3523431T (pt) * 2016-10-05 2021-01-04 Syngenta Participations Ag Melhoramentos no ou relacionados com o silenciamento de genes
CN109735573A (zh) * 2019-02-19 2019-05-10 武汉普诺赛生命科技有限公司 一种瞬时转染试剂及其应用方法
EP3798250A1 (fr) * 2019-09-25 2021-03-31 University College Dublin Polymères cationiques hyperramifiés utiles en tant que vecteurs d'administration d'acide nucléique pour la transfection
CN112195193A (zh) * 2020-09-30 2021-01-08 上海交通大学 单链dna在基因转染中的应用
CN114904003B (zh) * 2021-02-09 2023-09-29 广州立得生物医药科技有限公司 可电离的阳离子脂质类似物材料在作为核酸药物递送载体或转染试剂中的应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003509060A (ja) * 1999-09-17 2003-03-11 ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ 逆トランスフェクション方法
WO2004065636A1 (fr) * 2003-01-13 2004-08-05 Nitto Denko Corporation Surface solide pour administration de biomolecules et dosage a rendement eleve
WO2004076557A1 (fr) * 2003-02-25 2004-09-10 Nitto Denko Corporation Polyacetals biodegradables
JP2007534317A (ja) * 2004-02-18 2007-11-29 日東電工株式会社 真核細胞トランスフェクションのための培養装置および方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020006664A1 (en) * 1999-09-17 2002-01-17 Sabatini David M. Arrayed transfection method and uses related thereto
CA2427916C (fr) * 2000-11-03 2012-01-17 The Regents Of The University Of Michigan Transfection de surface et protocole d'expression
US20030215395A1 (en) * 2002-05-14 2003-11-20 Lei Yu Controllably degradable polymeric biomolecule or drug carrier and method of synthesizing said carrier
EP1513538A4 (fr) * 2002-06-14 2007-08-22 Mirus Bio Corp Nouveaux procedes d'apport de polynucleotides dans des cellules
US20040048260A1 (en) * 2002-09-10 2004-03-11 Fu-Hsiung Chang Transfection of nucleic acid
GB0320627D0 (en) * 2003-09-03 2003-10-01 Imp College Innovations Ltd Methods
US7125709B2 (en) * 2004-02-10 2006-10-24 Nitto Denko Corporation Culture device and method for eukaryotic cell transfection

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003509060A (ja) * 1999-09-17 2003-03-11 ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ 逆トランスフェクション方法
WO2004065636A1 (fr) * 2003-01-13 2004-08-05 Nitto Denko Corporation Surface solide pour administration de biomolecules et dosage a rendement eleve
WO2004076557A1 (fr) * 2003-02-25 2004-09-10 Nitto Denko Corporation Polyacetals biodegradables
JP2007534317A (ja) * 2004-02-18 2007-11-29 日東電工株式会社 真核細胞トランスフェクションのための培養装置および方法

Also Published As

Publication number Publication date
EP1836293A2 (fr) 2007-09-26
KR101263173B1 (ko) 2013-05-13
KR20070101859A (ko) 2007-10-17
KR20130010036A (ko) 2013-01-24
CA2590820A1 (fr) 2006-06-22
AU2005316501B2 (en) 2010-10-21
WO2006066001A3 (fr) 2006-09-08
CN101124316A (zh) 2008-02-13
WO2006066001A2 (fr) 2006-06-22
AU2005316501A1 (en) 2006-06-22
JP2008523810A (ja) 2008-07-10

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