KR101263173B1 - 진핵 세포의 트랜스펙션을 위한 고정화된 분해가능한양이온성 중합체 - Google Patents

진핵 세포의 트랜스펙션을 위한 고정화된 분해가능한양이온성 중합체 Download PDF

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KR101263173B1
KR101263173B1 KR1020077015797A KR20077015797A KR101263173B1 KR 101263173 B1 KR101263173 B1 KR 101263173B1 KR 1020077015797 A KR1020077015797 A KR 1020077015797A KR 20077015797 A KR20077015797 A KR 20077015797A KR 101263173 B1 KR101263173 B1 KR 101263173B1
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transfection
cells
cell
formula
degradable
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KR1020077015797A
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KR20070101859A (ko
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야스노부 다나까
크리스 피. 카스텔로
레이 유
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닛토덴코 가부시키가이샤
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020077015797A 2004-12-17 2005-12-14 진핵 세포의 트랜스펙션을 위한 고정화된 분해가능한양이온성 중합체 KR101263173B1 (ko)

Applications Claiming Priority (2)

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US63734404P 2004-12-17 2004-12-17
US60/637,344 2004-12-17

Related Child Applications (1)

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KR1020137000063A Division KR20130010036A (ko) 2004-12-17 2005-12-14 진핵 세포의 트랜스펙션을 위한 고정화된 분해가능한 양이온성 중합체

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KR20070101859A KR20070101859A (ko) 2007-10-17
KR101263173B1 true KR101263173B1 (ko) 2013-05-13

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KR1020077015797A KR101263173B1 (ko) 2004-12-17 2005-12-14 진핵 세포의 트랜스펙션을 위한 고정화된 분해가능한양이온성 중합체
KR1020137000063A KR20130010036A (ko) 2004-12-17 2005-12-14 진핵 세포의 트랜스펙션을 위한 고정화된 분해가능한 양이온성 중합체

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Country Status (7)

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EP (1) EP1836293A2 (fr)
JP (1) JP4739352B2 (fr)
KR (2) KR101263173B1 (fr)
CN (1) CN101124316A (fr)
AU (1) AU2005316501B2 (fr)
CA (1) CA2590820A1 (fr)
WO (1) WO2006066001A2 (fr)

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US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
EP3718635A1 (fr) 2003-07-31 2020-10-07 Handylab, Inc. Traitement d'échantillons contenant des particules
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
EP1774017B1 (fr) 2004-07-26 2013-05-15 Pfenex Inc. Procede permettant d'ameliorer l'expression d'une proteine par mise au point d'une souche par genie genetique
US7358223B2 (en) 2004-10-04 2008-04-15 Nitto Denko Corporation Biodegradable cationic polymers
US9572886B2 (en) 2005-12-22 2017-02-21 Nitto Denko Corporation Agent for treating myelofibrosis
EP2001990B1 (fr) 2006-03-24 2016-06-29 Handylab, Inc. Système intégré de traitement d'échantillons microfluides et son procédé d'utilisation
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
WO2008061165A2 (fr) 2006-11-14 2008-05-22 Handylab, Inc. Cartouche microfluidique et son procédé de fabrication
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
WO2008134461A2 (fr) 2007-04-27 2008-11-06 Dow Global Technologies, Inc. Procédé pour rapidement cribler des hôtes microbiens et identifier certaines souches ayant un rendement et/ou une qualité d'expression des protéines hétérologues améliorés
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
ES2648798T3 (es) * 2007-07-13 2018-01-08 Handylab, Inc. Materiales de captura de polinucleótidos y métodos de utilización de los mismos
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
BR112013026451B1 (pt) 2011-04-15 2021-02-09 Becton, Dickinson And Company sistema e método para realizar ensaios de diagnóstico molecular em várias amostras em paralelo e simultaneamente amplificação em tempo real em pluralidade de câmaras de reação de amplificação
JP6117217B2 (ja) 2011-09-30 2017-04-19 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ ユニット化された試薬ストリップ
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
EP2773892B1 (fr) 2011-11-04 2020-10-07 Handylab, Inc. Dispositif de préparation d'échantillons de polynucléotides
BR112014018995B1 (pt) 2012-02-03 2021-01-19 Becton, Dickson And Company sistemas para executar ensaio automatizado
CN102827756B (zh) * 2012-06-25 2014-04-09 华南农业大学 应用于大豆下胚轴外植体转化受体系统的农杆菌局部侵染方法和装置
CN105579584B (zh) * 2013-06-28 2020-08-28 埃泽瑞斯公司 用于将rna引入细胞的组合物
US20180214572A1 (en) * 2014-11-10 2018-08-02 Ethris Gmbh Induction of osteogenesis by delivering bmp encoding rna
PT3523431T (pt) * 2016-10-05 2021-01-04 Syngenta Participations Ag Melhoramentos no ou relacionados com o silenciamento de genes
CN109735573A (zh) * 2019-02-19 2019-05-10 武汉普诺赛生命科技有限公司 一种瞬时转染试剂及其应用方法
EP3798250A1 (fr) * 2019-09-25 2021-03-31 University College Dublin Polymères cationiques hyperramifiés utiles en tant que vecteurs d'administration d'acide nucléique pour la transfection
CN112195193A (zh) * 2020-09-30 2021-01-08 上海交通大学 单链dna在基因转染中的应用
CN114904003B (zh) * 2021-02-09 2023-09-29 广州立得生物医药科技有限公司 可电离的阳离子脂质类似物材料在作为核酸药物递送载体或转染试剂中的应用

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US20030235916A1 (en) * 2002-06-14 2003-12-25 Monahan Sean D. Novel methods for the delivery of polynucleotides to cells
EP1398042A1 (fr) * 2002-09-10 2004-03-17 Lipotek, Inc Transfection d' acides nucleiques

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US20030215395A1 (en) * 2002-05-14 2003-11-20 Lei Yu Controllably degradable polymeric biomolecule or drug carrier and method of synthesizing said carrier
US20030235916A1 (en) * 2002-06-14 2003-12-25 Monahan Sean D. Novel methods for the delivery of polynucleotides to cells
EP1398042A1 (fr) * 2002-09-10 2004-03-17 Lipotek, Inc Transfection d' acides nucleiques

Also Published As

Publication number Publication date
EP1836293A2 (fr) 2007-09-26
KR20070101859A (ko) 2007-10-17
KR20130010036A (ko) 2013-01-24
JP4739352B2 (ja) 2011-08-03
CA2590820A1 (fr) 2006-06-22
AU2005316501B2 (en) 2010-10-21
WO2006066001A3 (fr) 2006-09-08
CN101124316A (zh) 2008-02-13
WO2006066001A2 (fr) 2006-06-22
AU2005316501A1 (en) 2006-06-22
JP2008523810A (ja) 2008-07-10

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