JP4553424B2 - Cosmetic composition - Google Patents

Description

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（処方例２８）被覆保護剤
ガーゼ又はリニメント布にフローブランカ熱水抽出液，抗生物質，抗炎症など適量を混合した処方液を含浸させ、外傷部に添付する。又、フローブランカ抽出物を直接、局所に散布し、ガーゼなどで被覆しても良い。
【０１３８】
（試験８）使用効果試験
本発明の化粧料組成物を実際に使用した場合の効果について検討を行った。使用テストはシミ，ソバカス，色黒で悩む人及び乾燥ぎみやツヤ・張りのない肌で悩む人３０〜６０歳の各１０名をパネラーとし、毎日、朝と夜の２回、洗顔後に処方例１の乳液の適量を顔面に３ヶ月に渡って塗布することにより行った。尚、対照には、乳液から本発明の各植物抽出物を除いたものを同様な方法にて処方したものを用いた。又、評価方法は下記の基準にて行い、結果は表１及び表２の通りで表中の数値は人数を表す。尚、使用期間中に皮膚の異常を訴えた者はなかった。
【０１３９】
「シミ・ソバカス＆皮膚色改善効果」
有 効：シミ・ソバカスや肌の色が白く、軽減された。
やや有効：シミ・ソバカスや肌の色が白く、やや軽減された。
無 効：使用前と変化なし。
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「皮膚感触改善効果」
有 効：乾燥肌や肌のツヤ・張りが増し、肌が改善された。
やや有効：乾燥肌や肌のツヤ・張りがやや増し、肌が改善された。
無 効：使用前と変化なし。
【０１４２】

【０１４３】
（試験結果）
表１及び表２の通り、本発明のバルバスコ、フローブランカの各植物抽出物を配合した乳液は、有意に乾燥肌を改善し、肌にツヤ・張りを与え、又、シミ，ソバカスや肌の色も白く、軽減することが確認できた。
【０１４４】
（試験９）使用効果試験
本発明の美肌化粧料組成物を実際に使用した場合の効果について検討を行った。使用テストは湿疹・肌荒れで悩む人、３０〜５０歳の各１０名をパネラーとし、毎日、朝と夜の２回、洗顔後に処方例１の乳液の適量を顔面に３ヶ月に渡って塗布することにより行った。尚、対照には、乳液から本発明の各植物抽出物を除いたものを同様な方法にて処方したものを用いた。又、評価方法は下記の基準にて行い、結果は表３の通りで表中の数値は人数を表す。尚、使用期間中に皮膚の異常を訴えた者はなかった。
【０１４５】
「美肌効果」
有 効：湿疹・肌荒れが改善された。
やや有効：湿疹・肌荒れが少し改善された。
無 効：使用前と変化なし。
【０１４６】

【０１４７】
（試験結果）
表３の通り、本発明のバルバスコ、フローブランカの各植物抽出物を配合した乳液は、湿疹・肌荒れを改善することが確認された。
【０１４８】
【発明の効果】
本発明のバルバスコ、フローブランカから選ばれる１種以上の植物抽出物を含有したメラニン生成抑制剤、活性酸素消去剤、過酸化脂質生成抑制剤、抗アレルギー剤、ヒスタミン遊離抑制剤又は化粧料組成物は、優れたメラニン生成抑制作用を有し、美白効果も確認され、又、活性酸素も有意に消去・抑制すること、又、過酸化脂質の生成を抑制すること。更に、ヒスタミン遊離抑制作用を確認した。そして、肌に対してもシミ、ソバカスを軽減すること。又、皮膚炎症性疾患（例えば、発赤、浮腫、湿疹など）・肌荒れ・乾燥肌の改善、更に、肌にツヤ・張りも与えることができ、人・動物に対しても安全なものである。尚、本発明は、その他一般的な飲食品類への利用も可能である。
【図面の簡単な説明】
【図１】エルカンプレ、バルバスコ、フローブランカの各植物抽出物のメラニン生成量に対する抑制率を示す図である。
【図２】エルカンプレ、バルバスコ、フローブランカの各植物抽出物の活性酸素消去作用を示す図である。
【図３】エルカンプレ、バルバスコ、フローブランカの各植物抽出物の過酸化脂質生成抑制作用を示す図である。
【図４】エルカンプレ、バルバスコ、フローブランカの各植物抽出物のヒスタミン遊離抑制作用を示す図である。[0001]
BACKGROUND OF THE INVENTION
The present inventionBarbasco, Flow BlancaThe present invention relates to melanin production inhibitor, active oxygen scavenger, lipid peroxide production inhibitor, antiallergic agent, histamine release inhibitor or cosmetic composition containing one or more plant extracts selected from .
[0002]
The field of use can be used in various external preparations (including preparations used for animals) in general, specifically, ampoules, capsules, pills, tablets, powders, granules, solids, liquids, gels Or 1) pharmaceuticals, 2) quasi-drugs, 3) topical or systemic skin cosmetics (for example, basic cosmetics such as lotions, emulsions, creams, ointments, lotions, oils, packs, etc.) Facial cleanser, skin cleanser, massage agent, cleansing agent, hair remover, hair remover, shaving treatment, after shave lotion, pre-show lotion, shaving cream, foundation, lipstick, blusher, eye shadow, eyeliner, mascara 4) Medicinal or / and cosmetic preparations applied to the scalp and hair (eg shampoos, rinses, treatments, perms) Liquids, hair dyes, hair conditioners, hair styling agents, hair growth / hair nourishing agents, etc.), 5) bathing agents used in bath water, 6) other anti-smell and deodorants, deodorants, hygiene products, Sanitary cotton, wet tissue, mouth freshener (for example, toothpaste, mouthwash, etc.) and the like.
[0003]
[Prior art]
Factors that determine human skin color include the amount of melanin in the epidermis, blood flow in capillaries, food-derived pigments (carotene), and the thickness of the stratum corneum. One is melanin pigment. The so-called liver spots (spots), sparrow eggs (sobacus), and skin pigmentation after sunburn are markedly enhanced production of melanin pigments due to the activation of pigment cells in the skin. It has become one of the major skin problems for women.
[0004]
This melanin pigment is produced by melanosomes, which are organelles in pigment cells (melanocytes) present in the basal layer of the epidermis, the hair root, and the outer root sheath. In the process of melanin pigment production, tyrosinase acts on tyrosine in pigment cells (melanocytes) to oxidize tyrosine, convert it into dopa and dopaquinone, and further auto-oxidize to produce dopachrome and 5,6-dihydroxyindole. After that, it polymerizes and eventually becomes a melanin pigment. The produced melanin is secreted from the dendrites of pigment cells (melanocytes) to the basal cells, and the basal cells divide and become spiny cells, then rises, reaches the stratum corneum, and then drops off with detachment of the stratum corneum. It is supposed to go.
[0005]
Therefore, in order to prevent or improve skin spots (spots), sparrow eggspot (sobacus) and darkness, inhibit the melanin production process. Alternatively, it is conceivable to lighten already produced melanin. Therefore, various whitening components have been proposed based on these ideas. For example, kojic acid or its derivative, ascorbic acid or its derivative, tocopherol or its derivative, glutathione, hydrogen peroxide, zinc peroxide, hydroquinone monobenzyl ether, placenta extract, silk extract or plant extract (Arnica, Altea , Aloe, oxon, enamel, chamomile, licorice, gardenia, genus shochu, sikon, shouma, birch, nematode, genus shochu, psycho, yamacha flower, toki, dragonflower, elderberry, safflower, garlic, pearl beetle, logwood, etc.) It was known.
[0006]
In recent years, the production of active oxygen in the living body and various effects (diseases) caused thereby have been reported. In general, this active oxygen is also called Activated oxygens.^2-, H₂O₂, OH, and chemiluminescence, each of which has a strong bactericidal action and is regarded as an important substance involved in the self-defense of living organisms. Enter the body, neutrophils, monocytes, and macrophages, which are phagocytes in the blood, start phagocytosis, and then lyse the phagocytic foreign bodies in the phagocytic vesicles. For this reason, active oxygen is produced, and this produced active oxygen not only dissolves sputum food, but on the other hand, it directly acts as a bactericidal and protects against foreign enemies such as bacteria and foreign substances. Playing a role. In other words, it has the function of eliminating antigenic substances before the immune function is activated.
[0007]
However, if this active oxygen for self-protection is excessively produced and secreted, even normal cells are lysed and stimulated, resulting in various disordered reactions. Recently, many diseases and illnesses induced by active oxygen have been reported. For example, fats and oils such as cholesterol in the blood are oxidized by active oxygen, and vascular damage (stroke, myocardial infarction, etc.) caused by arteriosclerosis in the brain and heart. It is said to cause blood flow disturbance due to thrombus and the like, and ischemia in blood vessels due to rapid transient atrophy during the brain trauma, resulting in an oxygen deficient state. Therefore, in this state, the xanthine dehydrogenase in the blood vessel is changed to xanthine oxidase, and a large amount of active oxygen is generated in the blood in the blood vessel, causing damage to the blood vessel wall and causing fatal damage. Yes.
[0008]
As for the relationship between the skin and active oxygen, for example, since the skin is an organ that directly contacts the outside world, the active oxygen continues to be excessive in the skin due to ultraviolet rays, radiation, etc. while being susceptible to environmental factors. Then, it reacts with the unsaturated fatty acid of the biological membrane phospholipid and lipid peroxide is produced. The generated lipid peroxide causes arteriosclerosis, carcinogenesis, aging, membrane destruction, protein denaturation, etc., and also causes skin inflammation, edema, wrinkles and the like. Furthermore, as a disease caused by excess of active oxygen, from a cosmetic viewpoint, for example, herpes dermatitis, X-ray dermatitis, burns, trauma, solar dermatitis, contact dermatitis, eczema, atopic dermatitis, etc. Therefore, in order to prevent or ameliorate various diseases so far, for example, in the living body of animals and plants, superoxide is required. Dismutase (SOD), catalase, glutathioperoxidase, etc., tocopherol (vitamin E), oryzanol, plant extract (hamamelis, melissa, birch, birch, sage, rosemary, ages, basil, ginkgo, psycho, peonies, hanger, Keihi, Taiso, Ogon, carrot, licorice, ginger) It was. In addition, as lipid peroxide production inhibitors, β-carotene, plant extracts (sesame culture cells, Achacha, Hypericum, Hamamelis, Melissa, Enmezo, birch, sage, rosemary, Nanten, Ajitsu, Ginkgo, green tea), etc. It was known.
[0009]
On the other hand, allergies such as hay fever, allergic rhinitis, bronchial asthma, atopic dermatitis, urticaria due to drugs, etc. due to recent deterioration of environmental conditions, lifestyle changes, and increased stress accompanying social life complexity An increasing number of people suffer from diseases, rough skin, and dry skin. In particular, atopic dermatitis has been attracting attention as one of the modern diseases because its increase has been screamed in a wide range of ages ranging from children to adults.
[0010]
Originally, in order to eliminate foreign substances (bacteria, pollen, mites, etc .: antigens) that have invaded the body, immunity that works to protect the body by producing biological components (antibodies, lymphocytes, etc.) against it It has a function. However, sometimes the reaction becomes so sensitive that it can be harmful to the body and cause various diseases. This disordered reaction due to immune function is called allergy and is classified into immediate type and delayed type.
[0011]
Among allergic reactions, those with a high onset frequency are immediate types, and reactions involving mainly immunoglobulin E (IgE) antibodies. This IgE is produced against allergens that have invaded the body and has a strong effector effect on mast cells and basophils. Mast cells and basophils (a type of white blood cell) are histamine and A cell having granules containing a pharmacologically active amine such as serotonin. The former is present in the vicinity of blood vessels and connective tissue, and the latter is present in blood. On these cell membranes, there is a receptor that binds IgE antibody, and it has the property of binding strongly to IgE antibody, and when allergens of the same kind bind to IgE antibody bound to mast cells and basophils again. Along with degranulation, many active substances such as histamine and serotonin mentioned above are released from these cells, causing various allergic symptoms, redness and blistering rashes (urticaria) with itching on the skin, nose and eyes Symptoms that cause inflammation and itching and nasal discharge and tear secretion become active, or symptoms that cause tracheal congestion and respiratory distress (bronchial asthma) are classified as allergic diseases of this type ing.
[0012]
In the case of immediate allergy, the process can be broadly divided into three stages, classified according to the respective action points, and attempts have been made to prevent, alleviate, or treat allergic diseases by drug administration. . In other words, when foreign antigens enter the body, IgE antibodies are produced by the immunocompetent cell line, and IgE antibodies are distributed in mast cells distributed in the site of allergic reactions such as the respiratory tract, skin, digestive organs, or in the blood. Sensitization is established by adhering to basophils. This is the reaction referred to as the first stage.
[0013]
Next, when the antigen is brought into contact with the sensitized cells again, the cells undergo morphological changes such as vacuolation, swelling, and degranulation, and release chemical transmitters called histamine, serotonin, SRS-A, and the like. This is the second stage reaction.
[0014]
The released chemical transmitter acts on the contraction of smooth muscles such as bronchial muscle and digestive tract, increased capillary permeability, neutrophil migration, platelet aggregation, etc., resulting in asthma, low back pain and diarrhea The process of developing allergic symptoms such as gastrointestinal allergies, nasal allergies and urticaria is the third stage reaction.
[0015]
Therefore, in a narrow sense, a drug that acts on the first and second stages can be regarded as a preventive agent for an allergic reaction, and a drug that acts on the third stage can be regarded as a symptomatic therapeutic agent. Currently, the most active research on antiallergic drugs is the development of drugs that suppress this second stage.
[0016]
[Problems to be solved by the invention]
However, the above-mentioned whitening ingredients, active oxygen scavenging substances, and lipid peroxide production inhibiting substances have poor stability and solubility in the prescription system, cause off-flavor and coloring due to starch and decomposition, and are effective at the living body level. In addition, there were problems in terms of safety, such as causing skin irritation, which was not satisfactory.
[0017]
In addition, many of the antiallergic agents that have been conventionally used are drugs related to diseases caused by immediate allergic reactions whose action points are relatively clear. Examples include antispasmodics that relax smooth muscles, sympathomimetics that suppress increased capillary permeability, and antihistamines, all of which are contrasting therapeutic agents that act in the third stage. Moreover, most of them are synthetic drugs, and there were problems in terms of side effects.
[0018]
On the other hand, research on antiallergic agents involved in immediate allergic diseases that are currently most actively conducted include drugs that suppress the release of chemical mediators, drugs that antagonize the released chemical mediators, etc. Although it is the development of a drug that suppresses the second stage, no drug having sufficient efficacy has been found.
[0019]
[Means for Solving the Problems]
In view of such circumstances, the present inventors set the theme of development of useful plants having melanin production inhibitory action, active oxygen scavenging (SOD) action, lipid peroxide production inhibitory action, antiallergic action (histamine release inhibitory action), as a result,Barbasco, Flow BlancaOne or more plant extracts selected from the group have a whitening effect that significantly suppresses the formation of melanin pigments. In addition, active oxygen should be significantly eliminated and suppressed. Also, suppress the production of lipid peroxide. Furthermore, the histamine release inhibitory action was confirmed. And to reduce spots and freckles on the skin. In addition, skin inflammatory diseases (for example, redness, edema, eczema, etc.), improvement of rough skin and dry skin, as well as highly safe melanin production inhibitor, active oxygen scavenger that can give skin gloss and tension, The present invention has been completed by providing a lipid peroxide production inhibitor, an antiallergic agent, a histamine release inhibitor or a cosmetic composition.
[0021]
DETAILED DESCRIPTION OF THE INVENTION
Used in the present invention“Barbasco” uses flowers, leaves, rhizomes, and roots of the Buddlejaceae and Buddleja plants: Barbasco: Buddleja thyrsoides Lam. Or Buddleja cambaraArech. The species Brazilian Buddleia (Carson de Vello: Buddleja brasiliensis Jacq.ex K.Sprengel or Buddleja australis Vell.) Can also be used.
[0022]
DETAILED DESCRIPTION OF THE INVENTION
Used in the present invention“Flow Blanca” means Amaranthaceae and Iresine plants: Flor Blance: Iresineweberbaueri Suess.) Use above-ground parts such as flowers, leaves, stems and bark.
[0023]
Used in the present inventionBarbasco, Flow BlancaThe extract of is obtained by pulverizing various parts (stems, leaves, stems, branches, or flowers, rhizomes, roots, bark, aerial parts, etc.) of each plant as they are or after pulverization. Alternatively, it is extracted as it is or after pulverization with a solvent.
[0024]
Examples of the extraction solvent include water, alcohols (for example, lower alcohols such as methanol, absolute ethanol, and ethanol, or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), ketones such as acetone, ethyl ether, and dioxane. , Esters such as acetonitrile and ethyl acetate, and organic solvents such as xylene, benzene and chloroform can be used alone or in any combination of two or more, and the solvent extracts are combined. But you can use it.
[0025]
The production method is not particularly limited, but is usually within the range of the boiling point of the solvent at normal temperature and normal pressure. After extraction, filtration or ion exchange resin is used for adsorption, decolorization, and purification. A solution, paste, gel, or powder may be used. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decolorization may be added as long as the effect is not affected. An activated carbon column or the like may be used, and usual means generally applied depending on the extracted substance may be arbitrarily selected.
[0026]
or,Barbasco, Flow BlancaVarious plant extracts are dried, concentrated, or diluted depending on the dosage form / form of the melanin production inhibitor, active oxygen scavenger, lipid peroxide production inhibitor, antiallergic agent, histamine release inhibitor, or cosmetic composition to be applied. Any adjustments may be made.
[0027]
Of the present inventionBarbasco, Flow BlancaThe extract can be used as it is as a melanin production inhibitor, active oxygen scavenger, lipid peroxide production inhibitor, antiallergic agent, histamine release inhibitor, and can be contained in cosmetic compositions. Melanin production inhibitor, active oxygen scavenger, lipid peroxide production inhibitor, antiallergic agent, histamine release inhibitor or cosmetic composition type, quality, and expected degree of action Usually, it is 0.001% by weight or more (hereinafter expressed as%), preferably 0.2 to 20%. If the content is less than 0.001%, the effect cannot be expected sufficiently.
[0028]
In addition, the melanin production inhibitor, active oxygen scavenger, lipid peroxide production inhibitor, antiallergic agent, histamine release inhibitor or cosmetic composition of the present invention is added to the above essential components as necessary. The components and additives exemplified below can be arbitrarily selected and used in combination as long as the effects are not impaired.
[0029]
(1) Various fats and oils
Avocado oil, almond oil, fennel oil, egoma oil, olive oil, orange oil, orange rafa oil, sesame oil, cacao butter, chamomile oil, carrot oil, cucumber oil, beef tallow fatty acid, kukui nut oil, safflower oil, shea fat, soybean oil , Camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, molasses, coconut oil, beef tallow, tallow or these oils and fats Hydrogenated products (hardened oil, etc.)
[0030]
(2) Wax
Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, rice wax, squalene, squalane, pristane, etc.
[0031]
(3) Mineral oil
Liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microkristane wax, etc.
[0032]
(4) Fatty acids
Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid , Synthetic fatty acids such as 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.
[0033]
(5) Alcohols
Natural alcohols such as ethanol, isopyropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, 2-octyldodecanol.
[0034]
(6) Polyhydric alcohols
Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1,3-butylene glycol Glycerin, pentaerythritol, sorbitol, mannitol, etc.
[0035]
(7) Esters
Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, cetyl lactate, myristyl lactate, diethyl phthalate, phthalate Dibutyl acid, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate and the like.
[0036]
(8) Metal soap
Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate, etc.
[0037]
(9) Gums, sugars or water-soluble polymer compounds
Gum arabic, benzoin gum, danmar gum, guaiac gum, Irish moss, Karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, lactose, fructose, sucrose or esters thereof, trehalose or derivatives thereof, dextrin, gelatin, pectin, starch, Carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) chitin or chitosan to which alkylene (C2-C4) oxide such as ethylene oxide is added, low molecular chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorus Oxidized chitin or chitosan, alginic acid or salt thereof, hyaluronic acid or salt thereof, chondroitin sulfate or salt thereof, heparin, ethylcellulose, methylcellulose, carboxymethylcellulose, carbo Polyalkylene oxide such as xyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyethylene oxide and polypropylene oxide or Its cross-linked polymer, carboxyvinyl polymer, polyethyleneimine, etc.
[0038]
(10) Surfactant
Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate ester salt, alkyl phosphate ester salt), cationic surfactant (alkylamine salt, alkyl quaternary ammonium salt), amphoteric surfactant: carboxylic acid Type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic surfactant) Agent, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants (natural surfactant, protein Hydrolyzed derivatives, polymer surfactants, surfactants containing titanium and silicon, fluorocarbon surfactants Such as.
[0039]
(11) Various vitamins
Vitamin A group: retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), Pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C group: ascorbic acid or derivatives thereof, vitamin D group: ergocalciferol (vitamin D2) , Cholecalciferol (vitamin D3), dihydrotaxosterol, vitamin E group: tocopherol or derivatives thereof, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K2), menadione (bi) Min K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, .gamma.-oryzanol, orotic acid, vitamin P such (rutin, eriocitrin, hesperidin), and vitamin U.
[0040]
(12) Various amino acids
Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine, ornithine, histidine And amino acid derivatives such as sulfate, phosphate, nitrate, citrate, or pyrrolidone carboxylic acid.
[0041]
(13) Various additives derived from plant or animal materials
These are processed in a conventional manner according to the product type and form to be added (for example, pulverization, milling, washing, hydrolysis, fermentation, purification, pressing, extraction, fractionation, filtration, drying, pulverization) , Granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, and the like are arbitrarily selected and combined), and may be arbitrarily selected from various materials.
[0042]
The solvent used for the extraction may be selected in consideration of the intended purpose and type of the product to be provided, or the processing performed later. Usually, water, a water-soluble organic solvent (for example, ethanol, propylene glycol) It is desirable to use one or a mixture of two or more selected from 1,3-butylene glycol and the like. However, when it is not preferable to contain an organic solvent depending on the application, it is sufficient to use only water or ethanol that can be easily removed after extraction, and it may be used alone or in any mixture with water. The thing extracted and extracted may be sufficient.
[0043]
In addition, when using additives derived from plant or animal-based ingredients for systemic or topical external preparations and cosmetics, it protects skin and hair, moisturizes, improves touch and texture, imparts flexibility, and stimulates Relaxation, stress relief by fragrance, cell activation (cell aging prevention), suppression of inflammation, improvement of skin and hair quality, prevention of rough skin and its improvement, hair growth, hair growth, hair loss prevention, imparting gloss, cleansing effect, In addition to cosmetic effects such as fatigue relief, blood flow promotion, and bathing effects, effects such as flavoring, deodorizing, thickening, preserving, and buffering can also be expected.
[0044]
Specific plants (herbal medicines) as raw materials include, for example, almonds (toen), ai (indigo leaves), aokazura (Keifu Fuji), Aoki (Aoki), Aogiri or Kenashia Ogiri (Ao Tung), Aotsu Samurai (wooden candy) , Acacia, Akashoma (red hempen), Akasuguri `` fruit '', Red grape, Akaminoki (Logwood), Akamegashiwa (Red bud moth), Akane (Agaric root), Akajia or Giou (Early yellow), Agi (Aki), Akikara pine, Akinare (Crust), Aguahe (Omanten palm), Akebii (Kido), Asa (Makojin), Malva morning glory or morning glory (Chicken), Hydrangea (hydrangea), Ashitaba (Tomorrow), Azuki (Red Azuki), Acerola, Asenyaku (Asen Yaku), Athira (Shokuyokanna), Anise, Abemaki `` Fruits '', Avocado, Ama, Amacha (Amacha), Achacharu, Amadokoro (Tamatake), Amana (Kojyo), Amaran Susus (Hyuyu, Hagaitoto, Higegateto, Senninkoku, Sugimorigateto, Hosoaogeito, Aogaetou, Haribi, Amaranthus Hippocondriacus), Amigasayuri or Baimo (Strauch), Arcana (Arcanna), Algarrobo (Cave), Altea Alka, Altea, Alca Katsudaidai (Almond), Aloe (Amber), Aloe Vera, Angelica, Apricot or Honanzu (Anzujin), Ansokkou (benzoic incense), Ikakouzolina (Gallhead), Ikarisou or Yatsuma Taikarisou (Inyoukaku), Igusa (Kanshinso), Itadori Tiger cane), yew (first place), strawberry, fig (no flower `` fruit, leaf ''), ichihatsu (first), ichibi (winter lion), ichiyakuso (one herb), ginkgo (ginkgo `` seed, leaf '') , Itohimehagi (distant), locust bean, Inazuna (Tailexi), Inubiyu (Honaga) Nubiyu), rice `` seed, seed coat '', Inondo `` seed '', Ibuki Sawweed, nettle, ylang ylang, iwa tobacco (Iwabuchi), iwahiba or iwamatsu (rolled mushroom), oolong tea, fennel (mud), wheat (chibusanoki), ukiyara (Sanryo), Warbler's fruit `` fruit '', Japanese cypress, turmeric (depressed gold), duckweed (float), Usbasaishin or Keirinsaishin or Oshusaishin (spicy), Usvenia oyster, Usbenitachiaoi, Nezubusa (Summer hay) , Udo or Shishido (Cultivation, Independence, Tang Dynasty), Unyadegato (Uncaria, Cat's Claw), Uba Tea, Ume (Plum Plum “Seeds, Flesh”), Vulgari, Uwaurushi (Culberry), Eustagon, Estragon (蝦 夷 蝦 夷加), Echinashi (Hosobamurasaki Barengiku), Enishida, Enokitake (ケ), Bisugusa or Cassia Tora (Keriko), Elderberry `` fruit '', Elemi, Elingi or Pre-Lotus Elinji, Enju (Aoihana, Aoi Rice), Ougi or Kibanaougi (Yellow), Ouren (Huangren), Okarasuuri (Callocon), Ooguruma (civil engineering incense), Otsuka Rafuji (preventive self), psyllium (car front child, car front grass), hazel (palm), large white pokeweed or pokeweed (white cocoon), large white beetle (Banaba), Obayayashabushi "fruit", Hawthorn or Hawthorn (Yamako), Ousei (Naruco Yuri, Kagura-mabanaru Yuri), Ohisano Yuki or Sasano Yuki, Barley (barley), Oohoshigusa (cereal essence), Oka, Okazeri (serpentine), Ogura (overturn), Okra Fruit ", ginseng or ginseng (carrot), hypericum or scabbard Hypericum (brothers cut grass), Odorikosou (continuous), Onamomi (Crows ears), Onigurumi, Onidokoro or Tokoro or Nagadokoro (hikai), Oninoyagara (Tempa), Onyuri or Sasayuri or Hakatayuri (Yuri), Onionis, Halibut ), Ominaeshi (defeated soy sauce), Yuuko, Dutch mustard (watercress), Dutch jellies, Dutch honey bees, olives "fruit, seeds, leaves", oregano, orange "fruits, pericarp", silkworm (mature yellow), cacao "fruits" , Pericarp, seeds '', oysters (柿蒂 `` leaves ''), scallops (Katsufuji 鈎), oysters or cantors (lotus), cassia, casserole (fruit berries), gadgets (莪 朮), oak (Tree, maple leaves), Cascarilla, Cascaraca grada, gypsophila, cynomolgus (Ginsato), Kaniwa, valerian (Valentine radix), birch or fern birch (white birch), pumpkin, mosquito Sea buckthorn `` seed '', kahoku salamander (蜀椒), catfish (蒲 yellow), chamomile or roman chamomile, chamomile (weed), cam cam (camo duck), red crested mane, crow cucumber or cinnamon crow (king moth), crow bishak (half-summer), Oat seeds, guarana `` seed '', calahoo (kokubaku), karaya, karin (kiso), garcinia, kawamidori, kawara-saiko (compulsed rape, white-billed grass), kawajisha, kawaratake, stone mulberry (stone bamboo) or Ezokawara radish (buckwheat, buckwheat) Child), Carrot Carrot (blue strawberry), Kawaramugi (Golden Chin), Kansui (sweet candy), Licorice (licorice), Kantara Asa, Candelilla, Ganto, Kanna, Raspberry (Ezo strawberry, Dutch strawberry, Shrimp strawberry, Navashiro strawberry, Momiji strawberry, Europe (Kistrawberry), Kiwi (fruit, leaves), Kikarasuuri (Gurone), Kikyo (kikyo) (Chrysanthemum root), chrysanthemum (Chrysanthemum flower), striped daisy, ginkgo biloba), catalpa (fruit), gypsophila (sheep), pheasant (carp), kizuta, yellow aloe vera, yellow mint, quina, linden (shinkona, red linden), quinoa (quinoa) ), Yellowfin, yellowtail, cabbage, cabbage `` unripe fruit '', cucumber, goryu (Nishikawa willow, taiyanagi), Kiraja saponaria, kiraya, Kiranso (golden acne grass), kumquat `` fruit '', betel, Goldfish (Sengakurusu), Guava `` fruit '', guayule, quercus infectria (garlic), kukui nuts, wolfberry (bamboo shoots, coconut leaves, cocoon leaves, husks), kusasu katsura (Tenmon winter), kudu (kudzu), camphor tree , Gooseberry "fruit", ginseng (yellow flower moth), gardenia (Yamashiko), kunugi (Sawada), Kupuas, Kumazasa, Kumamatsura (horse whip) ), Clara (bitter), cranberry `` fruit '', chestnut `` seed, fruit, astringent skin '', curcrigo latifolia `` fruit '', grapefruit `` fruit and leaf '', buckthorn, black-headed mochi (rescue), black-faced chick koji or long-tailed wolf (life extension) Grass), clove (chome, clove), gumbainazuna (sekimei, sekimeishi), keigai (bamboo shoot, coconut), keito (chicken flower, chicken cub), bay bay (bay laurel), kenashi sartorii rose (dough, mountain return), gentian , Gennoshouko (Oldweed Grass), Kemponashi (Kugushi), Kokisekokukoku, Kushimikan (Koji, Tachibana, Obenimikan, Fukuromikan, Sagamikouji, Ponkan, Santara (Tachibana Skin)), Kousinbara (Monthly Flower), Kousihakka, Kozo `` Fruit '', kocha (tea), kohone (river bone), kouhon (Tsubakimoto, Karatsumoto), Kouriyan, Koryo (fruit), Koenro (fruit), Koganen (yellow gong), Koganebana (yellow gong), Cowberry (Etsutachibana), Coco palm (fruit), Goshuyu (Kurean), Gosho strawberry (covered bonsai), Pepper (pepper), Copaiba balsam, coffee `` seed, leaves '', kobushi or magnolia (hot pepper), burdock (beef bowl, beef eggplant), kobotan, sesame (sesame seeds), sesame seedling (genus ginseng), garbage (seed quail), sanekazura or binankazura or pine bush, wheat (Wheat), rice or rice bran `` red cocoon, white birch '', rice oil, cola acminata `` seed '', cola vera `` seed '', fenugreek `` fruit '', colombo, kondurango, kombu, konjac, comfrey ), Sisal (Sisal Asa), Saxima button eel or Syna button eel or Chinese ginseng (wei Sensen), Sakura (Oshima cherry, Yamazakura, Oyama) Kura, Edhigashi, Bean cherry, Miyazakura, Yoshino cherry, Takane cherry, Kasami cherry, Chojizakura, Kohigan, Sato cherry, Kanzakura `` Leaf, flowers, fruit, bark (cherry bark) ''), cherry, pomegranate, Sasa, Sasanka, Sajimodaka Sweet potato, sugar cane, sugar beet, sunflower jujube (acid vine), saffron (bancho flower, west red flower), pomelo `` fruit '', savanna, salmon berry `` fruit '', salamander (sasmaki), salvia (sage), ragweed (mountain) Chinese cabbage), Sawagurumi (Yamamuro), Sangre de Grado (Crotone), Japanese horse mackerel, Sansine carrot (Ginseng ginseng), Sanshuyu (Japanese yam), Sansho (Japanese yam), Sandskon (Yamazu root), Shea (Kalite) ), Cyanoki "fruit", shiitake (shiitake), shion (purple grape), jikitarisu, shikunshi (messenger), shiso Is Aojiso or Chile-meiji or Katamen-jiso (Shiso leaf, Shiso-ko), Rosewood, Shinanoki, Shirahonoki, Sinarenkyo (ream), Shimeji (Hinshimeji, Shakashimeji, Hatake-shimeji, Oshiroi-Shimeji, Buna-shimeji, Honjimeshi, Shirotamotake, Shita-moe-takeshi (Glue), Shajin (Shasan), Jasmine (Matsurika), Janohige (Bakumon Winter), Shukukonkamiso, Shukushamitsu (Sanjin, Shredded Sandals), Juzudama, Shuro "fruit", Ginger (Ginger), Zhoushan Hydrangea (Tsuneyama), Shobu (bamboo root, Sone), Shozu "fruit", Shirashi "seed", Shirocho butterfly "seed", White-headed adzuki (chicken bone grass), Shirobanairis (Nioiris), Shirobanatsuta "flower", Shirominanten (southern sky) Fruit), cinchona sill sill bra, ginkgo biloba Flowers), cinnamon, cinnamum cassia (cinnamon), watermelon (western phoenix), honeysuckle (gold and silver flowers, shinofu), sorrel (acid mimic), swim berry "fruit", stevia, strawberry "fruit", succulent (Xu Longan), cedar ( Q & A), purslane (horse-toothed tendon, horse-toothed tendon), plum (fruit), Japanese red pine (cone), Japanese larch, Japanese walnut, Japanese walnut, Japanese hawthorn, Japanese dandelion, Japanese horse chestnut (Maroni), Japanese pear Fruits Root), secrecy (stone wall), cedro (Boushuboku), mallow, Hiroha Senega, Senega, Seri, Celery, Senkyu (river cucumber), Senshinren (Purex ream), Sendan, Senburi (Takumitsu), Senna `` fruit, leaf '', Senninsou (Daisen), Soka ( (Grass and fruit), buckwheat `` seed '', rice potato, daiou (daihuang), radish, soybean, daidai (orange peel, strawberry), takasaburo (bamboo lotus), takatodai (daikon), hawk abalone (dark spine), dark sweet cherry `` Fruits '', hollyhock, prickly-spotted thyme (thyme, perilla), tachidokoro (hikai), tachiyanagi, tamarind `` seed '', onion, ramshiba (hot pepper), taranoki `` fruit, leaves, root bark '', tarwi, tanjin (dansang), Dandelion (Shinko) or Shirobana dandelion or Mouko dandelion, danmar, cherry `` fruit '', chigaya `` fruit, root, bud '', chixetsu carrot (bamboo ginseng), chi , Chancapiedra (Coleoptera), Ginseng (Large Yellow), Ginseng (蕪 夷), Ginkgo biloba (艾), Choreimaitake (、), Chorogi, Primrose, Tsukuritake (mushroom), camellia, camellia, clover (Lacquered licorice), camellia (duck cocoon grass), vine azuki (red azuki), tsurudukudami (what neck moth), tsuruna (apricot), tsuruninjin (four leaves ginseng), azabuki, deiko, teuchigurumi, duberry `` fruit '', tengusa, tencha (甜 Tea), Tendai Yaku (glaze), Touga (winter coconut), Toka Kazura, Pepper (Ganban), Toki (Tochigi), Tokinsenka (Marigold), Tounabena (river cut), corn or corn hair (Southern cocoon hair) ), Mulberry (Sadako), Torindo or Chosenrindo (Ryuboku), Tokusa (wood bandits), Dokudami (ten drugs), Tokon (root) Toshishi or Mamedaoshi or Eurasian quail, eucommia (bark `` bark, leaf, root ''), ash (bark), tomato, tragacanth, triacanthus `` seed '', tormentilla, dronoki, trooaoi, nigerian berry `` fruit '', yam or yam (mountain) (Medicine), nagyidada (butcher bloom), naginata koju, nazuna, rapeseed, jujube or vertical bean (sword bean), jujube, jujube (large cocoon), naniwaibara (gold cherry blossom), navena (continuous), nameko, narco lily (yellow spirit) , Peanuts (peanuts), nanten (southern berries), namban karamushi (snacks), nymphs (nape), sagebrush (bitter melons), nikuzuku, kei or nikkei or ceylon nikkei or yabu nikkei (cinnamon) or keishi (katsushi), Chinese leek, elderberry (bone, `` fruit, flower, stem, leaf ''), garlic (large persimmon), nurde (penta), leek, green Japanese cypress or Nebu or Nebi or Nemurino or Digokubana (community), Japanese thistle (Daisetsu), Japanese rose (Nutoshi), Yarrow, Japanese pear (Plum), Novara, Peach, Palm, Pineapple `` Fruit '', Hibiscus (Busouge, Furimbushouge, Roselle) ), Hydra, Hakama Labosi (bone crush), Hakusen (white crust), Hakulberry `` fruit '', Hakobe (breeding), hazel (lion), Hashiridokoro (roth root), basil, lotus (lotus, lotus, lotus) ), Parsley (Netherlands jellies), hadakamugi, batata, wasp or madake (bamboo bamboo), patchouli, mint (thin load, light load leaf), pearl barley (yokuinin), hanasuge (knowing mother), banana, hana hakka, hanabishi (shitsuri, shishitsuri) ), Vanilla bins, papaya, leafhoppers, pahalobobo, hub "whole grass, stem, leaf", paprika, clam or honey bee Gou (Vulgaris), Hamasuge (Katsukiko), Hamabishi (Shitsuko), Hamanasu (Maika Flower), Hamaboufu (Hamaboshi), Hamamelis, Rose (Rose), Agaric (Agaric, White-faced Agaric, Usukimorinokasa), Haran, Pariurus (Prunus japonicus), Parietalia, Balsamina (Plum Reishi, Nigauri), Harnile (Crustacea, Coral Peel, Coral Leaves), Harlin Dou, Handaikai (Popular Sea), Panoki, Hagigi (Gardenfish), Hikageturinjin (Participant), Pu'er tea (general tea), pecan nuts, ganbanana (stone crabs, vines), pistachio, pistachio, beet, scabbard (stone crabs), scabbard fern, hinata taikozu (cow knee), cypress, Hiba, Castor, Sunflower, Pepper, Himeuzu (Tempo), Himegama (Kaori), Himematsutake (Kawariharatake, Hiromatsutake), Pimenta "fruit", Bia Shrimp, beakkyu, Japanese apricot `` fruit '', belude oyster, Hirohaokinagusa (bald bald eagle), loquat `` fruit, leaves, stem '', betel nut (large clothes skin, coconut), sweet pod (southern rattan), Japanese cypress, Japanese cypress Winter leaf), Fujibakama (Orchid), Fuji bean (Tanso), Grapes `` Fruits, pericarp, seeds, leaves '', Beech, Fuyumushinatsusatake (Cycium Natsweed), Brazilian licorice, Brazilian carrot, Blackcurrant `` Fruits '', Blackberry , Plum `` fruit '', fullceraria, blueberry (Prunus serrata), prunes, blond psyllium, bundo (mung bean), hazelnut, loofah, safflower (safflower), heneken, belladonna, berry `` fruit '', pervianburger, red-breasted or ixusa ( (Keiten), Boysenberry "fruit", scallop or sardine or nendo or hahagigi Chia (geumbako), spinach (Daffodil, acute pupa, perforated grass), spinach, bow-fu (windproof), spinach, physalis (Torone), honoki (Japanese rustic, magnolia), bokeh (wooden), Hosobanaokera ( Amber), Acer palmatum (Keiten 37), Azalea barrengiku, Bodaiju (bamboo tree), Button (peony `` flowers, leaves, stems, bark ''), Button pi (peony skin), Hop, Jojoba, Bordeaux, White berry `` Fruit '', Hononiku (meat jutsu, Daiki), Honsecek (iron skin stone, sparrow mountain stone 斛), Mizuru tennansho (Tennan star), Maitake (maiko), maou (mao), maca, macadamia nut, macri ( Seaweed), Magwa (mulberry white bark `` bark, leaf ''), Magnolia splengeri, mashua (Tama no sen haren, kyukonkinrenka), matatabi (wood tengu), matsukasa, pine `` leaf, bark, root '', Matsuhodo (茯苓), Matico (co (Don Shijo), Mayorum (Hanahakka), Malvano Jazine (Bitter), Mulberry `` Fruit '', Quince, Mango, Mangosteen, Manzanya (Andesca Mitsuru), Manshugurumi, Mandarin `` Fruit '', Mannentake (Ganoderma), Pheasant Fruit ''), Mishimasaiko (Shibahu), Mizuoobako or Mizuasagao (Dongweed Grass), Mizokakushi (Half-sided lotus), Misohagi (Senka-na), Michiyanagi or Niwayanagi (Henpo), Mitsugashi, Mitsuba, Midorihakka, Mimosa, Myouga , Miracle fruit `` fruit '', myrrh, mirabaran, wheat straw, mugweed, mugwort, mugwort (life-prolonging skin), munya, murasaki (purple root), murasakiki kibu (purple pearl), murasaki corn, murasaki kinafuji (Kunming) (Chicken blood wisteria), Swordfish (beneficial herb), Meowoggi, Melaroika, Merri , Merirot, Melon `` Fruit '', Moukomomogi, Mosouchiku, Moje (Pepper), Mokko (Mika), Momijibaio, Peach (Peach `` Leaf, Seeds, Flowers, Fruit ''), Palm, Morello Cherry `` Fruit '', Morohaya (Hemp), Yakawa Murasakiimo, Yakuchi (Michichi), Cornflower (Yagurumagi), Yagurumahakka, Yakon, Yashabushi (Yagari) or Himeya Shabushi or Oya Yashabushi `` Fruits, pericarps, fruit ears '', Yachiyanagi, Yatsude ), Mistletoe (willow infestation), Willow (Kawayanagi, Tachiyanagi, Weeping willow, Red willow, Pussy willow, Incolyana, Kinuyanagi, Koryanagi, Unryu willow, Miyama willow, Palm, Yamanagi, Obayakina, Taira `` Stem '', Yabgarashi, Yabukouji ( (Purple gold beef), Yabu tobacco (crane camellia, Tenmeisei), pokeweed (commercial land), Japanese alder (Yamamuro), Japanese yam (Yamamushi), Japanese mugwort, eucalyptus, Yukinoshita (tiger ear grass), Yucca or flevifolia, Yuzu "fruit '', Lily, sagebrush, mugwort (palm leaf), ligan (Raimaru), lime `` fruit '', rye, lacanca `` fruit '', raspberry `` leaf, fruit '', latania (chlamelia), lacquer or shallot (palm white), lavender, Longan (longan), agave (Aonu agave, fuchlin agave), ryokcha (green tea), apple `` fruit, seeds, leaves, roots '', gentian, lubas or suabisimus (cool), litchi (bamboo shoots, toothpick), lettuce ( Chisha), red currant "fruit", lemon "fruit", lemongrass, forsythia or forsythia (ream), forsythia, wax palm , Lokon (Yoshi, Sone), Logan Berry "fruit", Rosemary (Mannenrou), Rosehip (Novara), Wasabi, Waremokkou (ground).
[0045]
In addition, other seawaters such as sea salt, seawater dried products, dead sea or Atlantic or Pacific sea water (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud and mud (fango), such as Sea mud or mud in various regions such as Italy, France, Germany, Eifel and Franceburg (Contained components: silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, magnesium oxide, calcium oxide) Strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic, water).
[0046]
As seaweeds, seaweed [Green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Aonori (Usubaonori, Susiaoonori, Hiraonori), seaweed [Brown algae: Kombu, Macub Hosome Kombu, Mitsuishi Kombu), Wakame, Hirome, Aowakame, Giant Kelp (Macrocystis pirifera, Macrocystis integrifolia, Hijiki, Hibamata), seaweed [Red algae: Hijirimen, Maxa (Tengusa), Nettle, cypress, prickly pear, caterpillar, yatabegusa, yui-kiri, striped primrose, crested beetle, spruce rhinoceros, crested rhinoceros, giraffe rhinoceros, ginseng giraffe, horned mackerel, plover Data, carrageen (Yahazutsunomata), Ezotsunomata, Togetsunomata, Hirakotoji, Kotojitsunomata, Suginori, Shikin'nori, Kainori, Ibotsunomata, Yareusubanori, Kagiusubanori, Sujiusubanori, Haiusubanori, Akamomijinori] and the like as a representative.
[0047]
In addition, other algae such as green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Kuwanomimo: Kuwanomimo, Volbox: Ohigegawa, Volbox, Palmera, Yotsumeo, Aomido: Chrysanthemum, Aoumidro, Tirguimidro, Hibidoro: Hibidoro, Aosa: Anaaaosa, Amiaosa, Nagaaosa, Kawanori: Kawanori, Friccella, Shiogusa Magama genus: Magamata, Iwagata: Fusaikawa, Surichogata, Heraiwata, Kurokita, Hanemo, Genus: Mill, Chromel, Sakibutimil, Nagamil, Hira Lemon, Casanori genus: Casanori, Jusmo genus: Jujusmo, Tamajusmo, Mizojuzumo, Mika ヅ kimo, Korekaete, Tsutsumimo genus: Kyukougusa, Hitogususa genus: Humanegusa, Hirohatohigususa, Umushihimoe, Sakikimoe Sumiremo: Sumiremo, Hoshimamidro, Fushinashimidoro etc.).
[0048]
Cyanobacteria (Suizenjinori: Suizinori, Aoko, Nenjumo: Kawatake, Ishijelly, Hassai, Yuremo, Rasenmo (Spirulina): Spirulina, Trichodesmium (Iaciasio), etc.
[0049]
Brown alga , Sanadagusa: Sanadagusa, Fukurimamiji, Commongusa: Commongusa, Yahazugusa: Ezoyahaz, Yahazugusa, Uraboshiyahaz, Jigamusa: Jigammagusa, Umiuchiwa, Uruminus : Navarimo, Chasomen genus: Motsuki Chasomen, Matsumo genus: Matsumo, Nagamatsumo genus: Nagamatsumo, Okinawa mozuku: Okinawa mozuku, Nisefu Mozuku: Niseft mozuku, Fumozuku genus: Futomozuku, Ishimozuku genus: Ishimozuku, Kuromo genus: Kuromo, Nisemozuku genus: Nisemozuku, Mozuku genus: Mozuku, Ishigeu genus: Ishige, Iroro, Ichimegasa genus: Ichimegasa, zelkova genus: Cicada Umibosu, Urushigusu: Urushigusa, Keurushigusa, Tobaccogusa, Composita: Composita, Haberodomo: Haberodoki, Havanori: Habanori, Habanori: Habanori, Commonbukuro, Ezobukuro, Ezobukuro Chishima fukuronori genus: Chishima fukuronori, Kagomenori genus: Kagomenori, Muraridori genus: Murachidori, shark genus: shark zusa, genus genus: lobster, genus genus: Yokojima manori, mosquito Monogen genus: cynomolgus genus, fennel spider genus: fennel spider, genus turmo: genus turmeric, turtle genus: shark, genus genus sujime Barracuda, Trollocombu: Trollocombu, Antokume: Antokume, Kajime: Kajime, Tsuruarame, Kurome, Kikuishikombu: Kikuishicombu, Nejikcombu: Nejicombu, Kuroshiome: Kuroshiome, Nekoshikonbu, Aramei Genus: Ainuwakame, Chigaiso, Oniwakame Ezoi Shige: Ezoi Shige, Yabane Moku: Yabane Moku, Rappa Moku: Rappa Moku, Joromoku: Uganomok, Jolo Mok, Hiemoku, Honda Walla: Tamanashimoku, Isomoku, Nagashimamoku, Akamoku, Shidamoku, Honda Walla, Nejimoku, Narasamo, Mametawala, Tatsuri, Yatsumata Moku, Umitorano, Obamoku, Fushishimoku, Hakumokumoku ), Genus Ukimo: Ouki, genus Burukimo: burukimo, genus genus: mosquito mono).
[0050]
Red algae Genus: Sea urchins, Tsukumonori, Anemone moth Genus: Tamaitadaki, Kagikenori Genus: Kaginori, Kagikenori, Proboscis spp .: Himetengusa, Hytengususa, Oobusa, Nambugusa, Ko La, Yohira, Kinukusa, Hibirodo: Hibirodo, Himebiro, Isomumedo: Isommedo, Occadium: Rhizoidea, Ryumonsou: Ryumonso, Rana, Nara Genus: Hosobanaminohana, Naminohana, Sangomodoki: Ragaramomodoki, Shiogusagoromo: Shiogusagoromo, Iwanokawa: Etsukiiwanawakawa, Kainokawa , Sakuranori, Nikumukade, Tambanori, Tsurutsuru, Isonohana: Nurakusa, Cronouraxa, Oumkadenori, Hirakinto Genus: Hirakintoki, Matabou: Matabou, Kintoki: Chabokintoki, Kintoki, Matsunori, Konoeri, Tokamatsu, Hitomatsu, Kakeraito: Oobakintoki, Itofori: Itofori, Naganobane, Funori Genus: Coleoptera: crenata, crested genus: crimson, crested, crested, crested, crested genus: cicada, cicada, cicada Kalimenia, Honoho: Honoo, Hikagenoit: Hikagenoit, Usuginu, Niku Honoo: Nikko Honoo, Venice Nago: Venice Nago, Susca Veni: Susca Beni, Oka Muragusa spp .: Porcupine spp., Milin spp. Isodantsu: Isodantsu, Atsubanori: Atsubanori, Ogonori: Ogonori, Tsurushiramo, Shiramo, Ogonogori, Mizookonori, Birch, Tensusamodoki: Bee Genus: Cyprinus, Saimi: Caterpillar, Saimi, Hari, Genus: Pterosa: Spinach, Sugiori: Ikanoashi, Hosobonori, Nobonori, Surgeonfish Genus: black-backed ginkgo, red-backed ginkgo: red-billed genus, echinacea: eagles, red-tailed moth Spiraea goat, dallus genus: dallus, scorpion varieties, anadalus, genus webagusa, genus genus: benif chronori, genus genus: fushitsunagi, himefutsutsunagi, hirohagitsugigi, tsutsugigi , Hanegis, Amica, Egonori: Egonori, Futogigi, Saeda: Saeda, Chirimomid: Chirmomid, Coniferaceae: Habutaeenori, Nohanori, Suzushironori, Usbeni: Usbeni, Hasujiginu: Hasujiginu, Nagakonohanori Genus: Nagakonohanori, Sugiginu: Sugiginu, Abasbasginu, Haius Banori, Caiusus Nori Ayaginu: Aeginu, Dadia: Enasidadia, Shimadadia: Isohagi, Shimadadia, Dajimodoki: Dajimodoki, Otogusa: Mourotogususa, Fuyugusa, Makuri: Makri, Yanaginogi, Yuna, Yasugi nona, Yuna Sasabayanaginori, Sozo genus: Kurosozo, Kobusoso, Hanesozo, Sozonohana, Hanegusa: Hanegusa, Kehanegusa, Kozanemo: Kozanemo Murasaki, Hoso-Zanemo, Himegoke: Himegoke, Kurohimegoke, Hyodoshigu: Chrysanthemum, Hyodoshigusa, Usubahiodoshi, Isomegusa: Isomegususa, Suginasagigusu, Fukushima, Fuso : Kawamoku, Aokawa Mozuku, Himekawa Mozuku, Ideyugogome: Ideyukogome, Okichimozuku: Ochimodoku, Otosa, Chinorimo: Chinorimo, Chisujinori: Chisujinori, etc.).
[0051]
Axal algae (Shajikumo spp., Shiratamamo spp., Hoshitsurimo spp .: Hoshitsurimo, Rikunotamus spp., Flask spp .: Hime flaskmo, Chaboflaskmo, Tripera spp., Etc.), Yellow algae (Hikarimo spp .: Hikarimo, etc.), etc.
[0052]
In addition, animal-derived materials include poultry crown extract, cow / human placenta extract, pig / cow stomach, duodenum or intestinal extract or degradation products thereof, pig / cow spleen extract or its Degradation products, extracts of porcine and bovine brain tissue, water-soluble collagen, collagen derivatives such as acylated collagen, collagen hydrolysates, elastin, elastin hydrolysates, water-soluble elastin derivatives, keratin and degradation products thereof Derivatives, silk proteins and their degradation products or derivatives thereof, porcine and bovine blood cell protein degradation products (globin peptides), porcine and bovine hemoglobin degradation products (hemin, hematin, heme, protoheme, heme iron, etc.), milk, casein and Decomposition product thereof or derivatives thereof, skim milk powder and decomposition product thereof or derivatives thereof, lactoferrin or decomposition product thereof, egg component, fish decomposition product Etc..
[0053]
(14) Microbial culture metabolites
Yeast metabolite, yeast extract, natto metabolite, natto extract, rice fermented extract, rice bran (red koji, birch) fermented extract, euglena extract, lactic acid fermented raw milk and skim milk, trehalose or its derivatives, etc. .
[0054]
(15) α-hydroxy acids
Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid, etc.
[0055]
(16) Inorganic pigment
Silica, magnesium silicate, talc, kaolin, bentonite, mica, mica titanium, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide , Gunjo, chromium oxide, chromium hydroxide, carbon black, calamine and so on.
[0056]
(17) UV absorber / blocker
Benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, sodium 2-hydroxy-4-methoxybenzophenone-5-sulfonate, dihydroxydimethoxybenzophenone, dihydroxydimethoxybenzophenone-sulfone Acid sodium, 2,4-dihydroxybenzophenone, tetrahydroxybenzophenone, etc.), p-aminobenzoic acid derivatives (paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, octyl paradimethylaminobenzoate) ), Methoxycinnamic acid derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate, octyl paramethoxycinnamate, paramethoxy 2-ethoxyethyl cinnamate, sodium paramethoxycinnamate, potassium paramethoxycinnamate, di-2-methoxycinnamic acid mono-2-ethylhexanoate glyceryl, etc.), salicylic acid derivatives (octyl salicylate, salicylphenyl, homomenthyl salicylate, dipropylene glycol salicylate, ethylene salicylate) Glycol, myristyl salicylate, methyl salicylate, etc.), anthranilic acid derivatives (such as methyl anthranilate), urocanic acid derivatives (such as urocanic acid, ethyl urocanate), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, Pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nico Acid derivatives, vitamin B6 derivatives, umbelliferone, esculin, benzyl cinnamate, cinoxalate, oxybenzone, dioxybenzone, octabenzone, soribenzone, benzoresorcinol, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, escalol, Zinc oxide, talc, kaolin, etc.
[0057]
(18) Whitening agent
p-aminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, Allantoin derivatives, nicotinic acid derivatives, vitamin C or derivatives thereof, vitamin E or derivatives thereof, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine and the like.
[0058]
(19) Tyrosinase activity inhibitor
Ascorbic acid or its derivative, hydroquinone or its derivative (hydroquinone benzyl ether), kojic acid or its derivative, tocopherol or its derivative, N-acetyltyrosine or its derivative, glutathione, hydrogen peroxide, zinc peroxide, hydroquinone monobenzyl ether Tell, placenta extract, silk extract, plant extract (mulberry, toki, firewood, clara, mugwort, honeysuckle, yellowfin, dolphin, matsuhod, pearl barley, licorice, hop, hawthorn, eucalyptus, sorghum, altea, keihi, mankei, hamamelis , Yamaguwa, Lifelong Grass, Kikyo, Toshishi, Zetsuko, Ishibushi, Mao, Senkyu, Dokatsu, Psycho, Bowfu, Hamaboufu, Ogon, Peony, Peonies, Genpoko, Kakkon, Liquorice, Pyramid, Roe, Cimicifuga, safflower, green tea, black tea, Ali elixir), and the like.
[0059]
(20) Melanin pigment reduction / degradation substance
Phenylmercury hexachlorophene, mercuric oxide, mercuric chloride, hydrogen peroxide, zinc peroxide, hydroquinone or its derivatives (hydroquinone benzyl ether).
[0060]
(21) Turnover promotion / cell activator
Hydroquinone, Lactic acid bacteria extract, Placenta extract, Ganoderma extract, Vitamin A, Vitamin E, Allantoin, Spleen extract, Thymus extract, Yeast extract, Fermented milk extract, Plant extract (Aloe, Ogon, Horsetail, Gentian, Burdock, Shikon, Carrot, (Hamelis, Hop, Yokuinin, Odori Sou, Assembly, Toki, Tokinsenka, Achacha, Hypericum, Cucumber, Ginger, Rosemary, Parsley).
[0061]
(22) Astringent
Succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid (including catechin compounds) and the like.
[0062]
(23) Active oxygen scavenger
SOD, catalase, glutathione peroxidase, etc.
[0063]
(24) Antioxidants
Ascorbic acid or a salt thereof, stearic acid ester, tocopherol or an ester derivative thereof, nordihydrogua ceretenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole, propyl gallate, sesamol , Sesamorin, gossypol etc.
[0064]
(25) Lipid peroxide production inhibitor
β-carotene, plant extracts (sesame cultured cells, amacha, hypericum, clams, clove, melissa, enamel, birch, sage, rosemary, nanami, jitsu, ginkgo, green tea).
[0065]
(26) Anti-inflammatory agent
Itamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor, dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or a salt thereof, glycyrrhetinic acid or a salt thereof Salt, licorice extract, sicon extract, age extract, etc.
[0066]
(27) Antibacterial / disinfectant / disinfectant
Acrinol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or its hydrolyzate, alkyldiaminoethylglycine chloride solution, triclosan, sodium hypochlorite, chloramine T, salicy powder, iodine compound, iodoform, sorbin Acid or salt thereof, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p-chlorophenol, p-chloro-m-xylenol, p-chloro -M-cresol, thymol, phenethyl alcohol, o-phenylphenol, irgasan CH3565, halocarban, hexachlorophene, chlorohexidine, ethanol, methanol, i Sopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, phenoxyethanol, chlorobutanol, isopropylmethylphenol, nonionic surfactant (polyoxyethylene lauryl ether, polyoxyethylene nonyl phenyl ether, polyoxyethylene octyl phenyl ether, etc.), Amphoteric surfactants, anionic surfactants (sodium lauryl sulfate, potassium lauroyl sarcosine, etc.), cationic surfactants (cetyltrimethylammonium bromide, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride), formaldehyde, hexamine, brilliant green, Malachite Green, Crystal Violet, Jamal, Photosensitive Element 101, Photosensitive Element 201, Photosensitive Element 4 No. 1, N- long chain acyl basic amino acid derivative and its acid shark salts, zinc oxide, hinokitiol, etc. sophora root.
[0067]
(28) Moisturizer
Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin or chitosan derivative, pyrrolidone carboxylic acid or its salt, sodium lactate, mini sasanishiki extract, natto Metabolites, natto extract, etc.
[0068]
(29) Elastase activity inhibitor
Fluorophosphate diisopropyl, plant extracts (Ogon, Hypericum, Clara, Mulberry leaves, Keihi, Genshosho, Comfrey, Sage, Elderberry, Bodaige, Buttonpi), seaweed extract, etc.
[0069]
(30) Hair preparation
Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castari tincture, pepper tincture, pepper tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, sodium bromate, thioglycolic acid and the like.
[0070]
(31) Antiandrogen
Follicular hormones (estrone, estradiol, ethinyl estradiol, etc.), isoflavones, oxendron, etc.
[0071]
(32) Peripheral blood flow promoter
Vitamin E or its derivatives, assembly extract, garlic extract, carrot extract, aloe extract, gentian extract, toki extract, cephalanthin, carpronium chloride, minoxidil and the like.
[0072]
(33) Local stimulant
Pepper tincture, nonyl acid vanillamide, cantalis tincture, ginger tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.
[0073]
(34) Metabolic active agent
Photosensitizer 301, hinokitiol, pantothenic acid or derivatives thereof, allantoin, placenta extract, biotin, pentadecanoic acid glyceride and the like.
[0074]
(35) Antiseborrheic agent
Pyridoxine or its derivatives, sulfur, vitamin B6 and the like.
[0075]
(36) Keratolytic agent
Resorcin, salicylic acid, lactic acid, etc.
[0076]
(37) Oxidizing agent
Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, sodium pyrophosphate, sodium orthophosphate Sodium silicate hydrogen peroxide adduct, sodium sulfate sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract and the like.
[0077]
(38) Hair remover
Inorganic reducing agents such as strontium sulfate, sodium sulfide, barium sulfide, calcium sulfide, thioglycolic acid or its salts (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate, strontium thioglycolate) Such.
[0078]
(39) Hair swelling agent
Ethanolamine, urea, guanidine, etc.
[0079]
(40) Dye agent
5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'-iminodiphenol, 2,4-diaminophenoxyethanol , Hydrochloric acid 2,4-diaminophenol, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, orthoaminophenol, N-phenyl acetate Paraphenylenediamine, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-diamine, nitroparaphenylenediamine, paraaminophenol Paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol, picramic acid, sodium picramate, N, N'-bis (4-aminophenyl) -2,5-diamino-1,4-quinonediimine, 5- ( 2-hydroxyethylamino) -2-methylphenol, N-phenylparaphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorcresol sulfate, 2-amino-5-nitrophenol sulfate, orthoaminophenol sulfate, sulfuric acid Orthochloroparaphenylenediamine, sulfuric acid 4,4'-diaminodiphenylamine, sulfuric acid 2,4-diaminophenol, sulfuric acid toluene-2,5-diamine, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroiosulfate Tophenylenediamine, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol, diphenylamine, α-naphthol, hydroquinone, pyrogallol, fluorologlucin, gallic acid , Resorcin, tannic acid, sodium 2-hydroxy-5-nitro-2 ', 4'-diaminoazobenzozene-5'-sulfonate, hematein and the like.
[0080]
(41) Fragrance
Natural animal fragrances such as musk, civet, castrium, ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guayakwood essential oil, cumin essential oil, black letter Essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western peppermint essential oil, dairy perfume essential oil, Tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trubalsum essential oil, buttery essential oil, rose essential oil, palmarosa essential oil, cocoon essential oil, hiba essential oil, sandalwood essential oil, petit gren essential oil, bay essential oil, vetiva essential oil, bergamot essential oil, pe -Plant flavors such as balsam essential oil, boad rose essential oil, melamine essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, Japanese mint essential oil, and other synthetic flavors .
[0081]
(42) Dyes / colorants
Red cabbage dye, red rice dye, red dye, anato dye, scum dye, turmeric dye, enju dye, krill dye, amber dye, caramel, gold, silver, gardenia dye, corn dye, onion dye, tamarind dye, spirulina dye, Buckwheat whole pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.
[0082]
Others, hormones, sequestering agents, pH adjusters, chelating agents, antiseptic / antibacterial agents, cooling agents, stabilizers, emulsifiers, animal / plant proteins or their degradation products, animal / plant polysaccharides or the like Degradation product, animal / plant glycoprotein or its degradation product, anti-inflammatory agent / anti-allergic agent, wound treatment agent, foam / foaming agent, thickener, oral agent, deodorant / deodorant, bitter, seasoning Enzymes and the like are raised, and by using them together, various additive or synergistic effects can be expected.
[0083]
The dosage form of the melanin production inhibitor, active oxygen scavenger, lipid peroxide production inhibitor, antiallergic agent, histamine release inhibitor or cosmetic composition of the present invention is arbitrary, and capsules, powders, granules, solids , Liquids, gels, bubbles, emulsions, creams, ointments, sheets and the like.
[0084]
Specifically, for example, external pharmaceutical preparations, lotions, emulsions, creams, ointments, lotions, oils, packs, and other basic cosmetics, facial cleansers and skin cleansers, hair removers, hair removers, after shave lotions, preshaves Makeup such as lotion, shaving cream, shampoo, rinse, hair treatment, hair conditioner, perm, hair nick, hair dye, hair growth and hair restorer, foundation, lipstick, blusher, eye shadow, eyeliner, mascara, etc. Applicable to various products such as cosmetics, perfumes, bath preparations, toothpastes, mouth fresheners, gargles, deodorants, deodorants, sanitary cotton, wet tissue, etc. It can also be used.
[0085]
The melanin production inhibitor, active oxygen scavenger, lipid peroxide production inhibitor, antiallergic agent, histamine release inhibitor or cosmetic composition of the present invention may be added in advance, It may be added during the production, and may be appropriately selected in consideration of workability.
[0086]
【Example】
Hereinafter, production examples, test examples, and formulation examples will be described. However, the present invention is not limited to these examples.
[0087]
(Production Example 1)
Elcampre, Barbasco and Flow Blanca 100g each was dried with purified water (about 80 ° C) for about 5 hours, filtered and extracted (dry solids: Elcampre 0.5-2.0%, Barbasco 0.6) ~ 2.0%, flow blanker 0.8-2.5%) is obtained about 1.0-3.0 kg.
[0088]
(Production Example 2)
100g of each dried plant of El Campre, Barbasco and Flow Blanca is immersed in 50% ethanol solution, extracted at room temperature for 5 days and nights, and then filtered to extract (dry solid content: El Campre 0.5-2.0%, Barvasco 0.6 ~ 2.0%, flow blanker 0.8-2.5%) is obtained about 1.0-3.0 kg.
[0089]
(Production Example 3)
100g of dried plant of El Campre, Barbasco and Flow Blanca were gently heated and extracted at 50 ° C for 6 hours with 1L of 30% propylene glycol solution and 30% 1,3-butylene glycol solution in equal volume, and suction filtered. About 1.0 to 3.0 kg of an extract (dry solid content: Elcampre 0.5 to 2.0%, Barvasco 0.6 to 2.0%, Flow Blanca 0.8 to 2.5%) is obtained.
[0090]
(Test 1) Melanin production inhibition test
The present inventor decided to evaluate the melanin production inhibitory action based on B16 melanoma cells.
[0091]
"Test method and evaluation method"
The B16 melanoma cells pre-cultured for 24 hours are transferred to a fresh medium, and 0.5% of the sample is added thereto and cultured for 3 days. Next, the cultured cells are treated with trypsin, heated and dissolved in 1N-NaOH, 10% DMSO solution, and the absorbance at 420 nm is measured. In addition, distilled water was used as a blank instead of the sample, and the activity of the cells to which the sample was added at the same time was measured by the MTT reduction method (Tim Mosmann; Journal of Immunological Methods p55-63 (1983)). The MTT reduction activity of the sample-loaded cells was calculated with the MTT reduction activity of the cells as 1 unit. The amount of melanin in the sample was calculated as absorbance / MTT reducing activity (1 unit), and the inhibition rate (%) with respect to the amount of melanin produced by blank cells was determined by the following equation (Equation 1). The results are shown in FIG.
"sample"
The sample for the melanin production inhibitory action test was prepared by using the extract obtained in Production Example 2 of the present invention (solid content concentration: adjusted to 0.5%, but for Barbasco, solid content concentration: 0.2%). did. As a comparative sample, arbutin (manufactured by Sigma) was prepared under the same conditions and used in the test.
[0092]
<Equation 1>
Melanin production inhibition rate (%) = {1- (sample melanin amount / blank melanin amount)} × 100
[0093]
(Test results)
As shown in FIG.Barbasco, Flow BlancaEach plant extract was confirmed to have a melanin production-inhibiting action, although slightly weaker than arbutin, and can therefore be used effectively as a whitening agent.
[0094]
(Test 2) Active oxygen scavenging (SOD-like activity) action test
In recent years, many diseases and illnesses induced by active oxygen have been reported, and it has been pointed out that removal and elimination of active oxygen (SOD activity) leads to prevention of tissue damage. In this test, the extract obtained in Production Example 2 has the ability to eliminate active oxygen generated by the xanthine-xanthine oxidase system by the present NBT method (lipid peroxide experimental method, p. 144, medical and dental publishing). ).
[0095]
"Test method and evaluation method"
In a small test tube, 2.5 mL of 0.05 M sodium carbonate buffer (pH 10.2), 0.75 mM nitroblue tetrazolium (NBT), 3.0 mM xanthine, 3.0 mM disodium ethylenediaminetetraacetate, 0.15 W / V% bovine serum albumin, and sample Add 0.1 mL of each solution (the solvent for the control) and keep at 25 ° C. for 10 minutes, add 0.1 mL of an aqueous xanthine oxidase solution (adjusted so that the absorbance change of NBT in the control is about 0.3 / 20 minutes), Absorbance at 560 nm was measured.
In addition, a sample containing purified water instead of a sample was used as a control, a blank containing a reaction stop solution was set before adding xanthine oxidase for each sample and control, and the active oxygen elimination rate was calculated by the following formula (Equation 2). Asked. The results are shown in FIG.
"sample"
In addition, the sample of the active oxygen scavenging action test was subjected to the test using the extract (solid content concentration: adjusted to 0.1%) obtained in Production Example 2 of the present invention. As a comparative sample, a Tochu extract (manufactured by Ichimaru Falcos) was prepared under the same conditions and used in the test.
[0096]
<Equation 2>
Active oxygen elimination rate (%) = {1− (sample O.D. value−blank (sample) O.D. value) / (control O.D. value−blank (control) O.D. value)} × 100
[0097]
(Test results)
As shown in FIG.Barbasco, Flow BlancaIt was confirmed that each plant extract significantly suppressed active oxygen production and had an active oxygen scavenging action. In addition, it has been confirmed that it has a strong active oxygen scavenging action as compared with Tochu extract, and therefore can be effectively used as an active oxygen scavenger.
[0098]
(Test 3) Lipid peroxide production inhibition test
The lipid peroxide is a compound in which an unsaturated fatty acid, which is a constituent of lipid, is added with a peroxide, mainly hydroperoxide. These lipid peroxides cause damage to cell membranes in vivo, cause inactivation of various enzymes and denaturation of biological proteins, and have adverse effects on living tissues. As a result, it is thought to promote aging and lead to diseases such as cancerous changes. In the skin, accumulation of lipid peroxides caused by peroxidation of sebum changes the function of skin cell membranes and mitochondria. Liver patches, melanosis, primary irritation dermatitis, atopic dermatitis Or it is said to be a cause of various skin diseases such as skin aging (wrinkles, senile pigmentation, etc.) and rough skin, and suppression of this lipid peroxidation is regarded as important in dermatology. . In this test, with respect to the extract obtained in Production Example 1, the inhibitory effect on peroxidation of skin surface lipids and squalene by the iron-ascorbic acid system was measured by the TBA method (Analytical, Biochemistry. Vol.95, P351-358). (1979)).
[0099]
"Test method and evaluation method"
4.9 mL of 0.8% sodium lauryl sulfate solution in which 0.1% linolenic acid is dissolved is placed in a 9 mL screw bottle. Add 0.1 mL of the sample solution to this, and irradiate with UV light for 1 hour (Toshiba: 3 FL-20SE lamps and FL-20SBL lamps are connected in parallel at a distance of 15 cm), and then 1.0 mL of this solution is 4.5%. Add 0.07 mL of dibutylhydroxytoluene and add 1.0 mL of 0.67% thiobarbituric acid (TBA) / 15% acetic acid solution and heat at 95 ° C for 1 hour. After cooling, add 4 mL of 15% methanol-containing n-butanol solution, shake well, and then centrifuge (2000 rpm, 10 minutes). The absorbance at 532 nm of this n-butanol layer was measured and used as the amount of lipid peroxide.
In addition, the amount of lipid peroxide when the sample is added and irradiated with ultraviolet rays is a, the amount of lipid peroxide when the sample is added and not irradiated with ultraviolet rays is b, and ethanol is used instead of the sample to add ultraviolet rays. The lipid peroxide production inhibition rate is calculated by the following formula (Equation 3) as the lipid peroxide amount a ′ when irradiated, and the lipid peroxide amount b ′ when ultraviolet light is not irradiated by adding ethanol instead of the sample. Is shown in FIG.
"sample"
In addition, the sample of the lipid peroxide production inhibition test was subjected to the test using the extract (solid content concentration: adjusted to 0.5%) obtained in Production Example 2 of the present invention. As a comparative sample, a Tochu extract (manufactured by Ichimaru Falcos) was prepared under the same conditions and used in the test.
[0100]
<Equation 3>
Lipid peroxide production inhibition rate (%) = {1− (ab) / (a′−b ′)} × 100
[0101]
(Test results)
As shown in FIG.Barbasco, Flow BlancaIt was confirmed that each plant extract significantly suppressed the production of lipid peroxide and had a lipid peroxide production inhibitory action. In addition, it has been confirmed that it has a strong lipid peroxide production inhibitory effect as compared with Tochu extract, and therefore can be effectively used as an antioxidant.
[0102]
(Test 4) Histamine release inhibition test
In an immediate allergic reaction, chemical mediators such as histamine are released from mast cells or basophils sensitized in the second stage. Therefore, a substance that suppresses histamine release can be expected to have an antiallergic action. In this test, a test method (J. Soc. Cosmet. Japan, Vol. 25, No. 4, P. 246 (1992)) for releasing histamine from rat mast cells using compound 48/80, a histamine release reagent, The extract obtained in Production Example 2 was examined.
[0103]
"Test method and evaluation method"
a. sample
In addition, the sample of the histamine release inhibition test was subjected to the test using the extract obtained in Production Example 2 of the present invention (solid content concentration: adjusted to 0.5%). As a comparative sample, cromolyn (manufactured by Sigma) was prepared under the same conditions and used for the test.
b. Measurement of free histamine
To a 1.2 mL mast cell suspension collected from the abdominal cavity of a rat (Slc: Wister male rat, about 4-9 weeks old), add 0.2 mL of the sample and compound 48/80 to a final concentration of 1 μg / mL. Incubated for 15 minutes at ° C. After stopping the reaction by cooling with ice, the reaction solution is centrifuged, and histamine released from the supernatant is extracted and purified, colored with o-phthaldialdehyde, and measured for fluorescence absorbance at an excitation wavelength of 360 nm and a fluorescence wavelength of 450 nm. The histamine release inhibition rate was determined by the following formula (Equation 4), and the results are shown in FIG.
[0104]
<Equation 4>
Histamine release inhibition rate (%) = {1− (A−C / B−C)} × 100
A: When a sample is coexisted with mast cells and a histamine releaser is added
Fluorescence intensity of liberated histamine
B: Released when a histamine releasing agent was added to mast cells
Fluorescence intensity of histamine
C: Fluorescence intensity of histamine naturally released from mast cells
(A, B, and C are the measured values minus the blind values.)
[0105]
(Test results)
As shown in FIG.Barbasco, Flow BlancaEach plant extract was confirmed to have a higher histamine release inhibitory effect than cromolyn, and can be effectively used as an antiallergic agent.
[0106]
(Test 5) Safety test
(1) Skin primary irritation test
Each plant extract obtained in Production Examples 1 to 3 was prepared so that the solid content concentration was about 2.0%, and this was white shaved rabbit (female, 3 birds per group, weight around 2.3 kg) 2x2cm of skin)²0.03 mL per square was applied. Judgment was performed 24, 48, and 72 hours after application using the primary irritation scoring method with erythema and edema as indicators. As a result, in all animals, no erythema and edema were observed, and it was judged negative.
[0107]
(Test 6) Safety test
(2) Cumulative skin irritation test
Each plant extract obtained in Production Examples 1 to 3 is prepared with purified water so that the solid concentration is about 2.0%, and this is shaved on the flank (2 × 4 cm²) Hartley guinea pigs (female, 3 mice per group, body weight around 320 g) were applied once a day, 5 times a week, 0.5 mL / per animal. Application was for 2 weeks, and shaving was performed on the last application day of each week. Judgment was made on the day of each application and the day after the last application by the scoring method of primary irritation using erythema and edema as indices. As a result, in all animals, no erythema and edema were observed for 2 weeks after application, and it was judged negative.
[0108]
(Test 7) Safety test
(3) Single dose toxicity study
Each plant extract powder obtained in Production Examples 1 to 3 was adjusted with purified water to a concentration of 12.5%, and ddy mice (male and (Female, 1 group, 5 animals, 5 weeks of age) was orally administered with a sonde, and the onset and degree of toxic symptoms were observed over time. As a result, no abnormalities were observed in all mice for 14 days, and no abnormalities were found in the anatomical results. Therefore, LD₅₀Was 2,000 mg / kg or more, and judged to be a safe substance.
[0109]
(Formulation example) Manufacture of melanin production inhibitor, active oxygen scavenger, lipid peroxide production inhibitor, antiallergic agent, histamine release inhibitor or cosmetic composition
According to said evaluation result, the example of the formulation is shown below, but each formulation example may be what was manufactured by the conventional method in manufacture of each product, and showed only the compounding quantity. The present invention is not limited to these.
[0110]

[0111]

[0112]

[0113]

[0114]

[0115]

[0116]

[0117]

[0118]

[0119]

[0120]

[0121]

[0122]

[0123]

[0124]

[0125]

[0126]

[0127]

[0128]

[0129]

[0130]

[0131]

[0132]

[0133]

[0134]

[0135]

[0136]

[0137]
(Formulation example 28) Coating protective agent
A gauze or liniment cloth is impregnated with an appropriate amount of prescription liquid such as flow blanker hot water extract, antibiotics, anti-inflammation, and attached to the wound. Alternatively, the flow blanker extract may be directly applied locally and covered with gauze or the like.
[0138]
(Test 8) Use effect test
The effect when the cosmetic composition of the present invention was actually used was examined. The use test is paneled by 10 people aged 30-60 years who are suffering from stains, buckwheat, darkness and those who are suffering from dry skin, no gloss, and no skin tension. An appropriate amount of the emulsion 1 was applied to the face over 3 months. In addition, the thing which remove | excluded each plant extract of this invention from the emulsion by the same method was used for the control. Moreover, the evaluation method is performed according to the following criteria, and the results are as shown in Tables 1 and 2. The numerical values in the tables represent the number of people. No one complained of skin abnormalities during the period of use.
[0139]
“Blemish / Sobacus & Skin Color Improvement”
Effective: Blemish and freckles and skin color are white and reduced.
Slightly effective: white spots and skin color are white and slightly reduced.
Invalid: No change before use.
[0140]

[0141]
"Skin feel improvement effect"
Effective: Improved dry skin, gloss and firmness of the skin, and improved skin.
Slightly effective: Slightly increased dry skin and skin gloss and tension, and improved skin.
Invalid: No change before use.
[0142]

[0143]
(Test results)
As shown in Tables 1 and 2, the present inventionBarbasco, Flow BlancaIt was confirmed that the emulsion containing each plant extract significantly improved dry skin, gave the skin gloss and tension, and white spots, freckles and skin color were reduced.
[0144]
(Test 9) Use effect test
The effect at the time of actually using the skin care cosmetic composition of this invention was examined. The use test is for people who suffer from eczema and rough skin, each of 10 people aged 30 to 50 years as a panelist, and apply an appropriate amount of the emulsion of Formulation Example 1 to the face for 3 months after washing the face twice daily in the morning and evening. Was done. In addition, the thing which remove | excluded each plant extract of this invention from the emulsion by the same method was used for the control. The evaluation method is based on the following criteria, and the results are as shown in Table 3. The numerical values in the table represent the number of people. No one complained of skin abnormalities during the period of use.
[0145]
"Beautiful skin effect"
Effective: Eczema and rough skin were improved.
Slightly effective: Eczema and rough skin were slightly improved.
Invalid: No change before use.
[0146]

[0147]
(Test results)
As shown in Table 3, the present inventionBarbasco, Flow BlancaIt was confirmed that the emulsion containing each plant extract improved the eczema and rough skin.
[0148]
【The invention's effect】
Of the present inventionBarbasco, Flow BlancaMelanin production inhibitor, active oxygen scavenger, lipid peroxide production inhibitor, antiallergic agent, histamine release inhibitor or cosmetic composition containing one or more plant extracts selected from It has an action, a whitening effect is confirmed, and active oxygen is also significantly eliminated / suppressed, and production of lipid peroxide is suppressed. Furthermore, the histamine release inhibitory action was confirmed. And to reduce spots and freckles on the skin. In addition, skin inflammatory diseases (for example, redness, edema, eczema, etc.), rough skin and dry skin can be improved, and the skin can be given gloss and tension, which is safe for humans and animals. The present invention can be used for other general foods and drinks.
[Brief description of the drawings]
[Figure 1]]It is a figure which shows the suppression rate with respect to the amount of melanin production of each plant extract of El Campre, Barbasco, and Flow Blanca.
FIG.]It is a figure which shows the active oxygen scavenging action of each plant extract of El Campre, Barbasco, and Flow Blanca.
[Fig. 3]]It is a figure which shows the lipid peroxide production inhibitory effect of each plant extract of El Campre, Barbasco, and Flow Blanca.
FIG.]It is a figure which shows the histamine release inhibitory action of each plant extract of El Campre, Barbasco, and Flow Blanca.

Claims (3)

An active oxygen scavenger comprising an extract obtained from the above-ground part of a flow blanker . An antiallergic agent comprising an extract obtained from the above-ground part of a flow blanker . A cosmetic composition comprising at least one of the active oxygen scavenger and the antiallergic agent according to claim 1 or 2.

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