JP2000247897A - Cosmetic composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a cosmetic composition including melanin-formation inhibitor that suppress the formation of melanin by using the extracts from the plants selected from cinchona bark, iris, Polygonum multiflorum, clover. SOLUTION: This cosmetic composition includes the extracts from the plants selected from (A) Cinchona sussirubra Pavon L or the like in Cinchona L, Rubiaceae, (B) Acorus calamus linne Besser in Acorus L., Araceae, (C) Polygonum multiflorum Thunb. in Polygonum, Polygonaceae, (D) Syzygium aromaticum Merrill et Perry in Syzygium Gaertn., Myrtaceae, preferably in an amount of 0.2-20 wt.%.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a melanin production inhibitor containing at least one plant extract selected from linden, shobu, turdokudami, and clove, an active oxygen scavenger, an antiallergic agent, a histamine release inhibitor, The present invention relates to an anti-captive active agent and its application to various cosmetic compositions in the field of pharmaceuticals / quasi-drugs or cosmetics.

[0002] The field of application is, for example, applicable to all kinds of external preparations (including preparations for animals), and specifically, ampules, capsules, pills, tablets, powders, granules, solids, 1) Pharmaceuticals, 2) of liquid, gel or air bubbles
Quasi-drugs, 3) topical or systemic skin cosmetics (e.g., basic cosmetics such as lotions, emulsions, creams, ointments, lotions, oils, packs, facial cleansers and skin cleansers, massage agents) , Cleansing agent, depilatory, depilatory, shaving treatment, after-shave lotion, pre-show lotion, shaving cream, etc.),
4) Pharmaceutical or / and cosmetic preparations applied to the scalp / hair (eg, shampoos, rinses, treatments, permanent liquids, hair dyes, hair styling, hair tonics, hair growth / hair restoration, etc.), 5 ) Bath agents to be poured into bath water, 6) In addition, liquid odor and deodorant inhibitors, sanitary goods, sanitary cotton, wet tissues, etc. are listed.

[0003]

2. Description of the Related Art Factors that determine human skin color include the amount of melanin in the epidermis, blood flow in capillaries, pigments derived from food (carotene), and the thickness of the stratum corneum. One of the most important factors is melanin pigment. So-called liver spots (stains), sparrow egg spots (buckwheat),
Pigmentation of the skin after sunburn is one in which the production of melanin pigment is remarkably enhanced by the activation of pigment cells present in the skin, and this is one of the major concerns for middle-aged and elderly people and women.

[0004] The melanin pigment is produced by melanosomes, which are organelles in pigment cells (melanocytes) existing in the basal layer of the epidermis, the hair root, and the outer root sheath. In the process of melanin pigment production, tyrosine is activated by tyrosine in pigment cells (melanocytes) to oxidize tyrosine,
It is converted to dopa and dopaquinone, further autoxidized, polymerized via dopachrome and 5,6-dihydroxyindole, and finally turned into a melanin pigment. The produced melanin is secreted by the dendrites of pigment cells (melanocytes) into basal cells, which divide and rise as spinous cells, reach the stratum corneum, and fall off as the stratum corneum detaches. It is going to go.

[0005] Therefore, in order to prevent or improve skin spots, spots and freckles, and darkness of the skin, it is necessary to inhibit the production process of melanin. Alternatively, it is conceivable to make already generated melanin lighter. Therefore, various whitening components have been conventionally proposed based on these ideas. For example, kojic acid and its derivatives, ascorbic acid and its derivatives, tocopherol and its derivatives, glutathione, hydrogen peroxide, zinc peroxide, hydroquinone monobenzyl ether, placenta extract, silk extract and plant extracts (Arnica, Altea , Aloe, Japanese gourd, Japanese radish, chamomile, licorice, gardenia, genoshoko, sicon, ginger, birch, senkyu,
Gennoshoko, psycho, sasanqua, Toki, calendula, elderberry, safflower, garlic, adlay, litchi, logwood), etc. were known.

In recent years, the production of active oxygen in a living body and various effects (diseases) caused thereby have been reported. Generally, this active oxygen is also called Activated oxygens, and is roughly classified into four types, O ²⁻ , H ₂ O ₂ , .OH, and chemiluminescence. Captured as important substances involved in defense, for example, when foreign enemies (antigens) such as bacteria, viruses, and foreign substances enter the living body, phagocytic neutrophils, monocytes, and macrophages in the blood first phagocytose. Initiating action, then active oxygen is produced to dissolve the phagocytosed foreign material in the phagocytic endoplasmic reticulum, and this produced active oxygen is responsible for dissolving the phagocytosis, while It directly exerts a bactericidal action on external enemies such as bacteria and foreign substances, and plays a role of protecting them from external enemies. In other words, it has the function of eliminating antigenic substances before the immune function works.

[0007] However, if active oxygen for self-defense is produced and secreted in excess, even normal cells are lysed and stimulated, resulting in various damage reactions. Recently, many diseases and diseases caused by active oxygen have been reported. For example, fats and oils such as cholesterol in the blood are oxidized by active oxygen, and vascular disorders due to arteriosclerosis in the brain and heart (stroke, myocardial infarction, etc.) ) And brain trauma, it is said to cause impaired blood flow due to thrombus and the like, and to cause ischemic state in blood vessels due to rapid transient atrophy, thereby causing anoxia. Therefore, in this state, it is said that xanthine dehydrogenase in the blood vessel is converted to xanthine oxidase, which generates a large amount of active oxygen in the blood in the blood vessel, causing damage to the blood vessel wall and causing fatal damage I have.

Further, regarding the relationship between the skin and the active oxygen, for example, since the skin is an organ which is in direct contact with the outside world, the active oxygen is excessively exposed to the skin by ultraviolet rays or radiation while being susceptible to environmental factors. If the state continues, it reacts with the unsaturated fatty acids of the phospholipids of the biological membrane, and the lipid peroxides are generated. The generated lipid peroxide causes arteriosclerosis, carcinogenesis, aging, membrane destruction, protein denaturation, etc., and also causes skin inflammation, edema, wrinkles, and the like. Further, as a disease due to excess active oxygen, from a cosmetic point of view, for example, herpetic dermatitis, X-ray dermatitis, burns, trauma, solar dermatitis, contact dermatitis,
Eczema, atopic dermatitis, etc.
There is a need for a substance that eliminates excess active oxygen present in a living body. In order to prevent or improve various diseases, for example, in a living body of animals and plants, superoxide dismutase (SOD), catalase, Glutathioperoxidase and other tocopherols (vitamin E), oryzanol, plant extracts (hamamelis, melissa, emisoso, birch, sage, rosemary, age, basil, ginkgo, psycho, peonies, hange, cahig, tissou, ogon, carrot ,
Licorice, ginger) and the like were used.

On the other hand, due to the recent deterioration of environmental conditions, changes in lifestyles, and increased stress accompanying the complication of social life, hay fever, allergic rhinitis, bronchial asthma, atopic dermatitis, drug-induced urticaria, etc. More and more people suffer from allergic diseases, rough skin, dry skin and the like. In particular, atopic dermatitis has been attracting attention as one of the modern illnesses, with its increase being sought in not only children but also in a wide range of ages up to adults.

[0010] In order to eliminate foreign substances (antigens such as bacteria, pollen, ticks, etc.) that have entered the body, the body must protect the body by producing biological components (antibodies, lymphocytes, etc.) against it. It has an immune function that works. However, sometimes the reaction becomes too sensitive and harmful to the body,
It can cause various diseases. The disorder reaction caused by this immune function is called allergy, and is classified into immediate type and delayed type.

Among allergic reactions, those with a high frequency of onset are immediate forms (types I to III), mainly reactions involving immunoglobulin E (IgE) antibodies. This IgE is produced against an allergen that has entered the body,
It has a strong effector effect on mast cells and basophils. Mast cells and basophils (a type of leukocyte) are cells that have granules containing pharmacologically active amines such as histamine and serotonin. The tissue and the latter are present in the blood. On these cell membranes, receptors that bind IgE antibodies are present and have the property of strongly binding to IgE antibodies, and Ig bound to mast cells and basophils.
When the same type of allergen binds again to the E antibody, a number of active substances such as histamine and serotonin are released from these cells with degranulation, causing various allergic symptoms, and itching and redness and bulging of the skin Symptoms of rash (urticaria), inflammation of the nose and eyes and itching and increased secretion of nasal discharge and tears, or symptoms of choking of the trachea and causing attacks of dyspnea (bronchial asthma) ) Are classified as allergic diseases of this type.

In immediate allergy, the process can be roughly classified into three stages, and each stage is classified according to the point of action, and attempts are made to prevent, alleviate or treat allergic diseases by administering drugs or the like. I have been. That is, when a foreign antigen enters the body, IgE antibodies are produced by immunocompetent cell lines, and the IgE antibodies are distributed in airway, skin, digestive organs and other mast cells, or in blood. Sensitization is established by sticking to basophils.
This is the reaction referred to as the first step.

Next, when the sensitized cells are again contacted with an antigen, the cells undergo morphological changes such as vacuolation, swelling, and degranulation, and release chemical transmitters such as histamine, serotonin, and SRS-A. I do. This is the second stage reaction.

The released chemical messenger acts on contraction of smooth muscles such as bronchial muscles and gastrointestinal tract, enhancement of capillary permeability, migration of neutrophils, aggregation of platelets, and the like. The process of developing allergic symptoms such as digestive allergy with diarrhea, nasal allergy, and urticaria is the third stage reaction.

Therefore, in a narrow sense, drugs acting in the first and second stages are prophylactics for allergic reactions,
Those acting in stages can be considered as symptomatic treatments. Currently, the most active research on antiallergic drugs is the development of drugs that suppress this second stage.

[0016] Type II allergy is known as a damaging reaction caused by the destruction of tissue cells by IgG and IgM antibodies. IgG and IgM antibodies acting on this system significantly activate the complement system when linked to an antigen. (Effector action). The reaction mechanism is based on the fact that IgG and IgM antibodies bind to antigen cells such as microorganisms, thereby activating the complement system and destroying target cells.

The function of the complement system is to activate the protease system on target cells in a chain, form and destroy the membrane-damaging complex (MAC), and to generate a group of fragments called anaphylatoxins. It takes complex steps such as promoting histamine release from mast cells and attracting polymorphonuclear leukocytes and macrophages to promote foreign body treatment. However, such reactions are caused by virus-infected autologous cells, tissue cells or erythrocytes to which haptens (substances that are not antigenic in nature but produce antibodies when bound to proteins, etc.) or bacteria are bound or adhered. In an immune disease, even if the cells are in their own normal cells, the living body recognizes and causes them as antigen cells. Typical types II allergic diseases include aplastic anemia and hemolytic anemia.

[0018] Type III allergy is a reaction in which a large amount of an antigen-antibody conjugate formed by binding of an antibody to an antigen is deposited on a tissue and damaged. Antibodies involved in this reaction are mainly IgG antibodies, which are reactions involving many factors such as the complement system and polymorphonuclear leukocytes, similar to type II, but the difference from type II is that the antigen is not a cell but a substance Is a major feature. still,
The reaction mechanism is that the produced antigen-antibody conjugate is deposited on tissues such as blood vessels, kidneys, joints, and skin to activate the complement system (effector effect). As a result, a large amount of anaphylatoxin is produced, which increases vascular permeability, contracts smooth muscle, and promotes the release of histamine from mast cells, resulting in inflammation.

Various test methods have been proposed according to the allergic reaction types classified in this way, and approaches to factors effective for preventing or ameliorating allergic diseases have been actively conducted.

[0020]

However, the above-mentioned whitening component or active oxygen-eliminating substance has poor stability and solubility in a prescription system, and produces an unpleasant odor and coloring due to sedimentation and decomposition. Effect is not enough,
Furthermore, there was a problem in terms of safety, such as rash on the skin, and it was not satisfactory.

Many of the conventionally used antiallergic drugs are drugs relating to diseases caused by an immediate allergic reaction whose action point is relatively clear.
For example, for type I allergy, antispasmodics that relax smooth muscle, sympathomimetics that suppress the increase in capillary permeability, and antihistamines are listed. Although it is a drug that acts at a stage and is effective as a symptomatic treatment, most of it is a synthetic drug and has a problem in side effects. Furthermore, active research has been conducted on the development of a drug effective for II-III type allergy, but at present, no specific antiallergic agent has been found yet.

On the other hand, the most active research on antiallergic agents involved in immediate-type allergic diseases is a drug that suppresses the release of chemical mediators and exhibits an antagonistic effect on the released chemical mediators. The development of drugs that suppress the second stage, such as drugs, has not yet been found to have sufficient efficacy.

[0023]

In view of these circumstances, the present inventors have studied the effects of suppressing melanin production and eliminating active oxygen (SO
D) Useful plants with action and anti-allergic action (histamine release inhibitory action, anti-captive activity) are the theme of development, and as a result, linden, shobu, turdkudami,
One or more plant extracts selected from cloves significantly suppress the production of melanin pigment and have a whitening effect.
Also, active oxygen should be significantly eliminated and suppressed. Furthermore, a histamine release inhibitory action and an anti-complement activity were confirmed. And to reduce stains and freckles on the skin. or,
Skin inflammatory diseases (for example, redness, edema, eczema, etc.), improvement of rough and dry skin, and highly safe melanin production inhibitors or active oxygen scavengers that can give gloss and firmness to the skin. Allergic agent, histamine release inhibitor,
The present invention has been completed by providing an anti-captive active agent and a cosmetic composition.

[0024]

BEST MODE FOR CARRYING OUT THE INVENTION The term "kinole: golden rouge" used in the present invention means "Rubiaceae" and "Cinc".
hona L.) Plant: Red linden (Cinchona succirubra P
avon et Klotzch) branches, stems, roots, and bark are used.
d) Moens ex Trimen ”, (C. officinalis L.), (C. calysay
a Wedd.) can also be used.

"Shobu: iris" refers to the taro family (Ar
aceae), plants of the genus Acorus L .: A stem (Acorus calamus Linne Besser) stem, roots and rhizomes are used, and other plants of the same genus, A. gramineus S.
oland.) can also be used.

[0026] The term "Tsurukudami: What a crow" means a rhizome or root bark, root, tuber of Polygonaceae, a plant of the genus Polygonum (Polygonum multiflorum Thunb.).

The term "clove: clove, clove" refers to the family Myrtaceae and the genus Syzygium.
Gaertn.) Plant: Clove “Syzygium aromaticum (L.)
Merrill et Perry (= Eugenia caryophyllata Thunber
g) ”.

The extracts of linden, shobu, tsuldokudami, and clove used in the present invention include various parts (branches, stems, barks, or stems, roots, rhizomes, root bark, tuberous roots, buds, etc.) of each plant. ) As-is and then crushed and then exploited. Alternatively, it is extracted with a solvent as it is or after pulverization.

Examples of the extraction solvent include water and alcohols (for example, lower alcohols such as methanol and ethanol,
Or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), ketones such as acetone, esters such as diethyl ether, dioxane, acetonitrile, ethyl acetate, xylene, benzene,
Organic solvents such as chloroform can be used alone or in any combination of two or more.
Further, it can be used in a state where each solvent extract is combined.

Also, linden, shobu, tsuldokudami,
Clove each plant extract is applied, melanin production inhibitor, active oxygen scavenger, antiallergic agent, histamine release inhibitor, antitrapping agent, drying, concentration, or depending on the form and form of the cosmetic composition, or The dilution may be arbitrarily performed and adjusted.

Although the production method is not particularly limited, it is usually sufficient if it is within the range of the boiling point of the solvent at normal temperature to normal pressure. Purification may be performed to obtain a solution, paste, gel, or powder. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decolorization may be added as long as the effect is not affected.
An activated carbon column or the like may be used as a purification treatment means such as decolorization, and any ordinary means generally applied to the extracted substance may be selected.

Each of the plant extracts of the linden, shrub, turd kudami, and clove of the present invention comprises a melanin production inhibitor,
Active oxygen scavenger, antiallergic agent, histamine release inhibitor, anticaptive active agent, can be contained in cosmetic compositions, and the content thereof is not particularly limited. However, melanin production inhibitor, active oxygen scavenger , An antiallergic agent, a histamine release inhibitor, an anti-captive active agent, the type of cosmetic composition, the quality, and the degree of expected action are slightly different.
The content is 0.001% by weight or more (hereinafter, expressed as% by weight), preferably 0.2 to 20%. The content is 0.001%
If less, the effect cannot be expected sufficiently.

The melanin production inhibitor, active oxygen scavenger, antiallergic agent, histamine release inhibitor, anticaptor active agent, and cosmetic composition of the present invention may be added to the above essential components, if necessary. It can be produced by arbitrarily selecting and using components and additives used in pharmaceuticals, quasi-drugs, cosmetics and the like as long as the effects of the invention are not impaired.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil,
Cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow fatty acid, kukuinut oil, safflower oil, shea butter oil, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil , Mink oil, egg yolk oil, palm oil, palm kernel oil, mokuro, coconut oil, tallow, lard, or hydrogenated products of these oils (hardened oil, etc.).

(2) Waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, rice wax, squalene, squalane, pristane, etc.

(3) Mineral oil Liquid paraffin, vaseline, paraffin, ozokelide, ceresin, microcrystalline wax and the like.

(4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil and lanolin fatty acid, isononanoic acid, caproic acid , 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2
-Synthetic fatty acids such as ethylhexanoic acid and isopentanoic acid.

(5) Alcohols Ethanol, isopiranol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, and phytosterol; and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) Gum, saccharides and water-soluble polymer compounds Arabic gum, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose and its esters, trehalose and its derivatives, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or alkylene such as chitosan, ethylene oxide (C2-C4)
Oxide-added hydroxyalkyl (C2-C
4) chitin or chitosan, low molecular weight chitin or chitosan,
Chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate and its salt, heparin, ethylcellulose, methylcellulose, carboxymethylcellulose, carboxyethylcellulose, sodium carboxyethylcellulose, hydroxyethylcellulose , Hydroxypropylcellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinylpyrrolidone, polyvinyl methacrylate, polyacrylate, polyalkylene oxide such as polyethylene oxide and polypropylene oxide or a crosslinked polymer thereof, carboxyvinyl polymer, Polyethyleneimine and the like.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt) ), Amphoteric surfactant:
Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate type amphoteric surfactant, nonionic surfactant (ether type nonionic) Surfactants, ether ester type nonionic surfactants, ester type nonionic surfactants, block polymer type nonionic surfactants, nitrogen-containing nonionic surfactants), other surfactants (natural surfactants) , Derivatives of protein hydrolysates,
Polymer surfactant, surfactant containing titanium and silicon,
Fluorocarbon surfactants).

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A
1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B)
6), cyanocobalamin (vitamin B12), folic acids,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: ascorbic acid and its derivatives, vitamin D group: ergocalciferol (vitamin D2), cholecalciferol (vitamin D
3), dihydrotaxerol, vitamin E group: tocopherol and its derivatives, ubiquinones, vitamin K
Group: phytonadione (vitamin K1), menaquinone (vitamin K2), menadione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (Rutin, eriocitrin, hesperidin), vitamin U, etc.

(12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine and the like, and their sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These are processed in a conventional manner according to the type and form of the product to be added (for example, pulverization, milling, washing, hydrolysis, Fermentation, purification, squeezing, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decoloration, etc. You can choose to serve.

The solvent to be used for the extraction may be selected in consideration of the purpose and type of the product to be provided, the processing to be performed later, and the like. Usually, water and a water-soluble organic solvent (for example, ethanol) are used. , Propylene glycol, 1,3-butylene glycol, etc.).
However, when the content of the organic solvent is not preferable depending on the application, only water may be used, or ethanol which is easily removed after the extraction may be used, and may be used alone or in an arbitrary mixed solution with water. Exploited extract may be used.

In addition, additives derived from plant or animal raw materials are
When applied to systemic or topical external preparations and cosmetics, including protection of skin and hair, moisturizing, improvement of feel and texture,
Gives flexibility, relieves irritation, relieves stress due to aroma, activates cells (prevents cell aging), suppresses inflammation, improves skin and hair quality, prevents and improves rough skin, hair growth, hair growth, hair loss prevention, gloss Application, cleaning effect, alleviation of fatigue, promotion of blood flow,
In addition to cosmetic effects such as a warm bath effect, effects such as flavoring, deodorizing, thickening, preserving, and buffering can be expected.

Specific plants (crude drugs) as raw materials include:
For example, almond (Hen peach), eye (Aiba), blue oak
(Seifu Wisteria), Aoki (Aoki), Aogiri or Kenasia Ogiri (Ogi), Aotsu-la-Fuji (Kibo), Acacia, Akashoma (Akashoma), Akasuguri "fruit", Aka-grape, Akaminoki (Logwood), Akamega-shiwa (Akabu-Kashiwa) , Akane (Akane grass root), Acayanius or Zhiou (Jihuang), Agi (Awei), Akinire (Royu skin), Akebi (Kidori),
Asa (John Asako), Malva Asagao or Asagao (Toshiko),
Hydrangea (Hydrangea), Amacha (Amacha), Ashitaba (Asahiha), Amana (Kojingi), Azuki (Red azuki), Acerola, Asenyak (Asenyaku), Anise, Abemaki fruit, Avocado, Ama, Amacha (Amacha), Amachaper , Amadorokoro (Jade bamboo), Amaranthus (Amaranthus), Amaranthus hippochondriacus, Haribiyu, Amaranthus hippochondriacus, Amigasa lily or Bimbo, Altea or Arnica, Alpinia ), Aloe (log), aloe vera, angelica, apricot or hon apricot (almond jelly), ansokkou (benzuka), Igakouzolina (chiral bile head), epimedium or potato biloba (inyokaku), rush (light heart grass), knotweed (tiger stick) , Strawberry (first place), strawberry , Figs (fructose "fruits and leaves"), figs (first), ichibi (winter aiko), ichiyakusou (one medicinal herb), ginkgo (ginkgo "seed and leaves"),
Itohimemegi (Enshi), Carob, Carob bean (Telexy), Inubiyu (Honaguinubi), Rice “seed, seed coat”, Inondo “seed”, Ibuki-kusou-so, nettle, ylang-ylang, iwa-tobacco, iwahiba or iwamatsu , Oolong tea, Fennel,
Ukiyagara (Mitsuryo), Uguisukagura "fruit", Himeuikyo, turmeric (Utsukin), duckweed (buoyant), Usubasaishin or Keirinsaishin or Shousaisaishin (spicy), Usvenia oi, Usvenitachiaoi, Nepenthes (summer grass) ), Udo or Shishiudo (Qang, independent, Tang independent),
Japanese apricot (Ume “seed, pulp”), Vulgarine, Pleurotus japonicus (Walker), Unshuang mandarin (Chin skin), Estragon, Ezocogi (Ezo Goka), Echinashi (Hosobamurasakibarengiku), Echinida, Enokitake (Enotake mushroom) Tora (Keiko), Elderberry "fruit", Elemi, Elingy or Pre-Lotus Elinge, Enju (Sophora oleracea, Sophora oleracea), Ogi or Kibanaohagi
(Astragalus), Ouren (Yellow), Ookarasuri (Karokon),
Giant giant lumber (incense civil engineering), giant giant clam (self-defense), plantain
(Horuko), Toucan (Haruko), Oobanokera or Okera (white jitsu), Obayashishabushi "fruit", Oomisehas or Hawthorne (Yamasato), Ousei (Nalco lily, Kagikurumabanarukoyuri), Ohisasanoyuki, Sasanoki (Barley), giant croaker (grain sperm), okazeri (snake bed), oguruma (turnover), okra "fruit", panax ginseng or ginseng (ginseng), hypericum or hypericum perforatum or hypericum perforatum
(Brother's cut grass), Odorikosou (intermittent), Onomimomi (blue ears), Origurumi, Onidokoro or Tokoro or Nagadokoro (Hikai), Oninoyagara (Tenma), Onyuri or Sasasayuri or Hakatayuri (Yuri), Ononis, Halibut , Ominaesi (losing sauce), Dutch mustard (Watercress), Dutch jelly, Dutch honeysuckle, olive "fruit, seeds, leaves", oregano, orange "fruits, peel", caycaio (ripe yellow), cacao "fruits, peel, Seeds ”, oysters (persimmon“ leaves ”), kagi-kazura (crop hooks), oysters or lanterns (lotus grass), cassia, casina aki (pepper fruits“ fruits ”), gajutsu (grajujutsu), kashiwa Foliage), cascarilla, cascara sagrada, kanikusa (kinsato), valerian (valer), birch or weeping birch (white birch), pumpkin, kapok seeds, kahoksansho (pepper), Ma (bamboo), chamomile or roman chamomile, kamiyatsude (weed), kacamu (duckling), crowsuri / chinakarasuri (gold melon), karasubishak (midsummer), oak mulberry, guarana seeds, karahoo (thick), karaya, karin (Gourd melon), Garcinia, Kawamidori, Kawara Psycho (Koryo greens), Kawazusha, Kawaratake, Kawaranadeshiko (Ishitake), Ezokawaranadeshiko (Quarnut, Quumiko), Kawaranzin (blue gourd), Kawarayomogi (Inchinchinko) , Kansui
(Sweet), licorice (licorice), cantaraasa, candelilla,
Canto, Canna, Raspberry (Ezo strawberry, Dutch strawberry, Shrimp strawberry, Nawashiroi strawberry, Momiji strawberry, European strawberry), Kiwi "Fruits and leaves", Kikarasuri (Urorone), Kikyo (Kikyo, Kikyo root), Kiku (Chrysanthemum, Shimakaniku, Datura (Catella japonicus), Catalpa (Azumitsu), Rigid ridge (Yotei), Pheasant (Kikomi), Kizuta, Kidachi aloe, Kidachihakka, Yellowfin (Huangbai), Gymnema sylvesta, Cabbage, Cabbage "Immature fruit", Cucumber,
Gyo-ryu (Nishikawayanagi / Teriyanagi), Kiraja Saponaria, Kiraya, Kiranso (Kinraku grass), Kumquat "fruit", betel, kinmizukiki (Senaku grass), Guava "fruit", guayule, Quercus infectria (gallicia), Kukui nuts, wolfberry (Gugi, gugi, gugi leaf, ground bone bark), Kusagi Kazura (Tenmen Fuyu), Kuzu (Kakkon), camphor tree, gooseberry "fruit", Xining ginseng (yellow flower)
Gardenia, gardenia, black clover, black clover, black croaker, cranberry "fruit",
Chestnut "seed, fruit, astringent skin", Curculigo latifolia "fruit", grapefruit "fruit / leaf", buckthorn, black stalked mochi (rescue rescues), kurobana okiokoshi or hinokiokoshi (extended life grass / extended life skin), gumbinazuna (sekimei, sekimeishi) , Kaygai
(Cow flower, rooster), bay laurel, kenashisarutibara (Tokubukuryo, Yamakiko), gentiana, gennoshoko (Old Grass), kenponashi (Kigushi), Koukisekokukku, Kishumitsukan (Koji, Tachibana, Obenimikan, Hukuremican, Sagami Koji, Ponkan, Santara
(Tachibana skin)), Koshinbara (Monthly flower), Kosui mint, Kozo "fruit", Kocha (tea), Kouhone (river bone), Kouhon (straw, Kara straw), Kouriang, Koryokyo
(Kogaro), Cilantro “fruit”, Kourouen (Kohren), Koganebana (yellow gon), Cowberry (Koshibachi), Coconut “fruit”, Goshuyu (goshuyu), Goshoichigo (covered bunko),
Pepper, copaiba balsam, coffee "seed,
Leaf ”, kobushi or magnolia (hot spring), burdock (burdock, burdock), coconut pea, sesame (sesame), sesame seeds (gensen), goji (sesame), sanekazura or binankazura or pine bush, wheat (wheat), rice or rice bran "Red bran, white bran", rice oil, cola acuminata "seed", cola
Vera “seed”, fenugreek “fruit”, colombo, kondulango, kelp, konjac, comfrey (finnari grass), sisal (sisalasa), saxima button or cinnamon button or cinnamon pear (weird sac), sakura (oshima sakura, yamazakura, Oyamazakura, Ed Higashi,
Mamezakura, Miyamazakura, Someiyoshino, Takanezakura, Kasumizakura, Kura, Kohigan, Satozakura, Kanzakura "leaves, flowers, fruits, bark (cherry bark)"), cherries,
Pomegranate, Sasa, Sazanka, Sadomodaka (Sawaka), Sweet potato, Sugar cane, Sugar beet, Sunabet jujube
(Acid soju), saffron (banseki safflower, western safflower), pomelo "fruit", saponso, salmon berry "fruit", salicinosa (maso), salvia (sage), sawagikyo (sangansai), sawagurumi (sangan peach) , Panax ginseng, Panax ginseng (Sax carrot), Sanshuyu (sanshuyu), Sansho (Sansho), Sandscon (Yamazone), Shea (Karite), Cyanoki "fruit", Shiitake (Shitake mushroom), Shion (Shionen) ), Red squirrels, red clover (shikunshi), perilla or aodiso or chirimenjiso or catamendiso (perilla leaves, perilla), rosewood,
Linden, Chinese pine, Chinese forsythia (forsythia), shimeji (hinshimeji, shakashimeji, hatakeshimeji, ossiloishimeji, bunashimeji, shirotamogitake), shimotsukesou, potato, peonies (shasanyaku), shajin (shasan),
Jano-beard (Bakumon winter), Sukushamitsu (sand sand, sand compaction), Juzudama, Palm "fruit", Ginger (ginger), Jouzan hydrangea (Tsaneyama), Shozuku "fruit", Shirakashi "seed", Shirogoshi butterfly "seed" ”, White adzuki (chicken bone grass), white iris (small iris), white napta“ flower ”, white nananten (minamitenmi), cinchona sassilbura, ginkgo biloba (mizuka, mizukahana, kenchohana), cinnamon, watermelon (watermelon), honeysuckle (Gold and silver flowers, Shinobi winter), sorrel
(Acid model), swim berry “fruit”, stevia, strawberry “fruit”, suzu psycho (Xu Chang Lord), horsetail (Qing Ting), purslane (horse dent, dent tooth), plum “fruit”,
Pinus sylvestris "cone", larch, larch, walnuts, walnuts, hawthorn, dandelion, cinnamon (malonie), pear "fruits", swordfish, elder, and pine wood , Yarrow (milfoil), Yezobara, Y. euphoria, Y. mistletoe, Y. mint or Y. mint, Y. radishes, S. terrestris (Iris iris), ZE. Kyuu),
Senshinren (shashinren), sendan, assembly (this drug),
Senna "fruits and leaves", Senginsou (large tate), Soka (grass), buckwheat "seed", Somenimo, Rhubarb (daioh),
Radish, soybean, Daidai (orange peel, Kikomi), Takasaburo (Dried lotus grass), Takatodai (Daigaku), Takawarabi (Inu spine), Dark sweet cherry "fruit", Tachiaoi, Tachijakoso (thyme, Momoka), Tachidokoro ( Hikai), Twill willow, tamarind "seed", onion, tamashiba (hot spring), taro tree "fruit, leaf, root bark", tanjin (tansan),
Dandelion (Kabuko) or White dandelion or Moko dandelion, Danmar, Cherry "fruit", Chigaya "fruit,
Roots, buds ”, ginseng (bamboo ginseng), chicory, ginseng (gomi), ginseng (daisuki), ginseng (Wuyi), ginseng (carrots), ginseng, ginseng (bamboo) Chorei), Tsukimiso, Tsukutake mushroom (mushroom), Camellia, Camellia, Tsumexa (lacquer lacustrine), Commelina (duckling grass), Tsuruazuki
(Red azuki bean), crucian carp, crocodile carrot (four-leaf ginseng), camellia, daico, teuchigurumi, duberry "fruit", tengusa, tencha (tencha), tendaiyaku (crowd drug), touga (winter gourd), toukagizura, capsicum (Pan pepper), Touki (Toki), Tokinsenka (Marigold), Tonabena (River cut), Maize or corn hair (Nanbaru hair), Tomozumomochi (Female Sadako), Tododo-kosensendo (Ryu-buri), Tokusa (Pirate), Dokudami (jujutsu), konkon (root), Toshishi or mamedaoshi or scutellaria, eucommia (Moraka "bark, leaves, roots"), ash (qin bark), tomato, tragacanth, triacanthos "seed"
Offspring, Tormentilla, Dronoki, Trolo-aoi, Niseria berry “fruit”, Yam yam (Yakuyaku), Nagikadada (Butcher Bloom), Naginata Kouju, Nazuna, Rape, Natame bean or Natameki (sword bean), Natsumikan, Jujube (Dajutsu) ), Naniwaibara (Kanekurako), Navena (continuous), Nameko, Narco lily (Yellow), Peanut (peanut), Nanten (Minami Tenmi), Nambankaramushi (ramie),
Nigaki (bitter tree), bitter mugwort (bitter mite), nutmeg, kei or nikkei or ceylon nikkei or yabnikkei (cinnamon bark)
Or Keishi (Keishi), Chinese Chive (Niriko), Elderberry (Bone-bearing tree "fruits, flowers, stems, leaves") Garlic (garlic), Nurde (fivefolds), Leek, Nemoki or Nebu or Nebi or Nemurinoki or Jigokubana ), Thistle (daiji), wild rose (real fruit), yarrow, Japanese radish (maehu), wild rose, peach,
Palm, pineapple "fruit", hibiscus (Bussouge, Furinbussouge, Roselle), Hakamauraboshi (bone crushing supplement), Hakusen (ringworm skin), Huckleberry "Fruit", Hakobe (Horuko), Hashibami (Haruko), Hashidokoro
(Roth root), basil, lotus (lotus, lotus, lotus), parsley
(Netherland jelly), naked mussels, batata, bees or medakes (bamboo-boiled), patchouli, mint (lightly-loaded, lightly-leaved leaves), pearl barley (yokuninin), hanasuge (tomochimo), banana, hanamacka, hanabishi (situri, shissuri), vanilla bins ,
Papaya, Hakkogusa (rat koji), hub "whole plant, stem,
Leaf ”, paprika, hamago or honeysuckle (vine thorns), hamasuge (kobushiko), hamabishi (shikoriko), hamanas (maikai flower), hamaboufu (beach storm), hamamelis,
Rose, agaric, agaric, agaric
Usukimori no Kasa), Haran, Parietalia, Harnile
(Yellow, Yu white skin, Yuba), Handaikai (Giant Sea), Breadfruit, Hiougi (sun dried), Ginkgo Ginseng (party), Pecan Nut, Giganbana (Shirubi, Jinshasha), Hikikoshi (Enmeisou) Surviving skin), Hishi (Hishimi), Pistachio, Beet, Hitotsuba (Stone reed), Hitotsubashinenida, Hinata Kozuchi (cow knee), Hinoki, Hiba, Castor, Sunflower, Bell pepper, Himeuzu (Tamaoi), Himegama (Kagami) , Himematsutake (Kawariharatake, Hiromatsutake), Pimenta "fruit", juniper, beakyu, hiyu "fruit", belodo oyster, hirohaokinagusa (white man), loquat "fruit,
Leaves, stems ”, betel nut (large clothing skin, betel nut), sprout
(Southern rattan), butterbur, butterbur dandelion (winter flower, winter leaf), fujibakama (orchid grass), wisteria bean (flat bean), grape "fruit, peel, seeds, leaves", beech, beetle Brazilian carrot, black currant “fruit”, blackberry, plum “fruit”, flucellaria, blueberry (Prunus aegypti), prunes, blonde psyllium, bundou (green beans), loofah, safflower (safflower), henneken, belladonna, berry “ Fruits ”, Perbianberg, Crassophilus duckweed (Keiten), Boysenberry“ Fruits ”, Broomweed or Elderweed or Nendow or Hawakigki / Kokia (skin), Baldwink (Fengxian, acute child, osteoporotic grass), Bawfish (windbreak) ), Spinach, physalis (Torone), honoki (Japanese thick, park), bokeh
(Gourd melon), Hosovanaokera (blue ajutsu),
(Kageten 37), Hosobabarengiku, Bodaiju (Bodai tree),
Button (peony, peony skin), hop, jojoba, white berry “fruit”, hon-oniku (meat porcelain / daigei), hon-shimeji, hon-sekko (iron bark stone, sparrow mountain stone), meisulten nansha (tennan star), Maitake (maitake), ephedra (mao), macadamia nuts, macri (sea grass), maguwa ("mulberry white bark" bark, leaves "), magnolia or splengeri, matatabi (kiten tate), matsukasa, pine" leaf, bark,
Root ”, Matsuhodo (bukuryo), Mayorum (Hanaminka), Malvanojazine (Bitterfruit), Mulberry“ Fruit ”, Quince, Mango, Mangosteen, Mansugurumi, Mandarin“ Fruit ”, Mannentake (Lingzhi), Pheasant (Fruit“ Fruit ”) ), Mishima Saiko (Saiko), Mizuobako Mizuasagao (Ryugoshi), Mizokaku (Hanbe Lotus), Misohagi (Chikusenna), Miyamanagi or Niyanagi (Trend), Mitsugashiwa, Mitsuba, Midorihakka, Mimosa, Miyuga, Miracle Fruit "Fruit", Myrrh, Myrobaran, Mukuge (Mokuge), Mukunoki, Mokuroji, Murasaki (purple root), Murasakikisukibu / Omurasakishikibu (purple pearl), Murasaki maize, Murasaki natsufuji (Kunming chicken blood Fuji), Mehajiki (mass mother plant), Mebogi, Melaleuca, Melissa, Melillot, Melon "fruit", Mowberry mugwort, Moso Tick, woodworking (climate), maple bar rhubarb, peach (peach "leaves, seeds, flowers, fruit"), bean sprouts, Morello cherry "fruit",
Moloheiya (burlap), yakawamurasakiimo, yakuchi (masuchi), cornflower (cornflower), cornflower mint,
Yashabushi (sharp car) or Himeyashabushi or Obayashishabushi "fruit, peel, ears", yachiyanagi, yatsude (octagonal lantern), mistletoe (willow parasitism), willow (kawayanagi, tachinyanagi, weeping willow, akayanagi, nekoyanagi, okinoyagi, okinoyagi, okinoyagi, okinoyagi, oak Unryu willow, yamayama willow, yamayanagi, obanyanagi, tyrickinuyanagi, foxwood willow, dronoki), yanagitade "leaves and stems", yabugarashi, yabukouji (purple gold cow), yabu tobacco (tsuruisuri / tenmeisei), yamagobo hanno (Yamaharu), Bayberry (Plum skin), Bayberry, Eucalyptus, Yukinoshita (Tiger ear grass), Yucca or Flebifolia,
Yuzu "fruit", lily, yorogusa, mugwort (Aiba), ligan (lightning circle), lime "fruit", rye, lacanca "fruit", raspberry "leaf, fruit", ratania (clameria), rakkyoe shallot (scallop white) ,lavender,
Longan (longan flesh), agave (Aon agave, fukurin agave), ryocha (green tea), apple "fruit, seed, leaf, root", gentian, rubus or suavisimus (tenryu), litchi (lichi, lichi nucleus),
Lettuce (Chisha), Red currant "Fruit", Lemon "Fruit", Lemongrass, Forsythia (Forsythia: Forsythia, Chinese Forsythia), Astragalus, Waxy palm, Lokon (Yoshi: Azuki), Loganberry "Fruit", Rosemary (Mannenrou), Rosehip (Nobara), Wasabi, Waremokou (Jiyu) and the like.

Other seawater, for example, seawater salt, dried seawater, inorganic salts obtained from the Dead Sea or the Atlantic or Pacific Ocean (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud / mud (fango) , For example, sea mud or mud of various places such as Italian Fango, German Fango, Eifel Fango, Freiburg Fango, etc. (Ingredients: silicon dioxide, titanium dioxide, aluminum oxide, iron oxide,
Manganese oxide, sodium oxide, potassium oxide, magnesium oxide, calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic,
Water).

Examples of the seaweeds include seaweeds [green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Aonori (Usbaaonori, Susiaonori, Hiraaonori, Bouaonori, Hosoedaoonori), and seaweed [brown algae: Kombu algae: brown algae: brown algae: brown seaweed: , Resiliconibu, hosomekobu, mishitishibu), seaweed, hirome, blue seaweed, giant kelp (macrocystis pilifera, macrocystis integrifolia, neocitys luetokeana), hijiki, hibamata], seaweed [red algae: hijirimen, maxa ( Tengsa),
Hirakusa, Onyxa, Obakusa, Kataobaxa, Yatabegusa, Yuikiri, Shimatengusa, Tosakanori, Togekirinsai, Akumasakirinsai, Kirinsai, Byaksinkirinsai, Tsunomata, Obatsunomata, Tochaka, Tohkanota, Tojika , Ibotsunomata, Yaleus Banori, Kagius Banori,
Suzius Banori, Hyus Banori, Akamomininori] and the like.

Other algae such as green algae (Chlamydomonas spp .: Chlamydomonas sp., Akayukimo, Dunaliella spp .: Dunaliella spp., Chlorococcus spp .: Chlorococcus, Kwanomimo spp. Spirogyra sp.
Genus Fricciella, genus genus: medusa, asamidori ogusa, kawashiogusa, marimo, valonia: tamagobaronia, tamabaronia, magnum: tamatamamo, iwata: husaiwata, suricogita, heraiwirata, crocodile, crocus Sakibutimimil, Nagamil, Hiramil, and Casanori:
Casa nori, Juzumo
Mizojuzumo, Mizuazumimo, Korekaete, Tsutsumimo, Kikkogusa: Kikkogusa, Hitoexa: Hitoexa, Hirohanohiegusa, Usuhitoexa, Motsuhitoe, Sayamidro, Genus spp.

Blue-green algae (Genus Lynch: L., L., L., L .: L., mushrooms,
(Hassai, Yuremo, Racenmo (Spirulina) genus: Spirulina, Trichodesmium (Aiacacio), etc.).

Brown algae (Piraella spp .: Piraella spp., Shiomidoro spp.
Genus Isogawara, genus Isogasula, genus genus: Gunsenkurogashira, genus Kashirazaki, genus Kashirazaki, genus Muchimo: genus Muchimo, Hiramutimo, Kebelligsa, genus Amis. Artemisia spp.
Genus Matsumo: genus Matsumo, genus matsumo: genus sylvestris, genus okinawa, genus okinawa-mozuku, genus niseftomozuku: niseftomozuku, futomozuku: genus futomozuku, ishimozuku: ishimozuku, kuromo: kuromo, nisemozuku: genus, spruce, spruce Ishige, Irolo, Icmegasa: Ichimegasa, Keyari: Keyari, Umibos: Umibos, Urushigusa: Urushigusa,
Species of the genus Coleoptera, Tobacco planta, Laminaria genus: Laminaria genus: Habanomodo genus: Habanomodo genus, Habanori genus: Havanori, genus Habanori: genus Habanori, genus bukuro: common bukuro, Ezobukuro genus: Ezobukuro, Fukuronori genus: Fukuronori, Watanabe Genus Kagomenori: Kagomenori, Muraridori: Murachidori, Shakusugusa: Sharks,
Genus Genus: Genus Genus, Genus Genus: Genus Genus: Genus Genus, Genus Kayamonori, Genus Kayamono, Genus Genus: Genus Ligustrum, Genus Tsurumo: Genus Turrum, Genus Anana: Genus Anastasia, Genus Ligustrum: Sujime, Genus Missiscoma: Genus Genus Ligustrum, G. , Onikombu, Goheikombu, Nagakombu, Pea kelp, Ochimikombu, Torokombu genus: Torokombu, Antokume genus: Antokume, Kajime: Kajime, Tsuruarame, Kurome, Kikuishikomu: Genus Kikuishi, Nejirekomu: Neshilecomb, Kuroshiomeko : Nekoshikambu,
Alame: Alame, Ainuwakame: Ainuwakame, Chigaiso, Oniwakameezoishige: Ezoishige, Yabanemoku: Yabanemoku, Rapamoku: Rapamoku, Jiromoku: Uganomok, Jiromoku, Hiemomoku, Honamomaku, Tamamomaku, Tamamomoku Akamoku, Shidamoku, Hondawara, Nejimoku, Narasamo, Mametawara, Tatsukuri, Yasumatamoku, Umitranoo, Oobamoku, Fushizushimoku, Hahakimokum, Togemoku, Jolemoku, Saw-mokum, Obanoki-moku, Genus genus: Genus
Bulukimo, genus cynomolgus: cynomolgus etc.).

Red algae (Genus genus: Ushikenori, Funorinoushike, Genus Amanori: Asakusanori, Susabinori,
Genus Upirinori, Onimanori, Tasa, Filitasa, Benitasa, Rhodocorton: Milnoveni, Akebonomozuku: Akebonomozuku, Konahada: Haikonahada, Yogorekonahada, Aokonahada, Sea Species: Acer genus : Larch, Fusanori: Fusanori, Nisefusanori: Nisefusanori,
Sodegarami: sodegarami, rattles: rattle, hiraragara, hirohatamataki: hirohatamatamadaki, tamamaidaki: tamaitadaki, kakenokenori: kaginori, kakenenori, tengusa, kurobana, kurobana, swordfish, swordfish, squirrel Genus: hibiroudo, himehibiroudo,
Genus genus: genus isensis, genus genus:
Genus spruce, Ryumonsou: Ryumonsou, Hera Ryumon, Nisekarequigusa: Nisekarequigusa, Okitsubara genus: Obaokitsubara, Akaba: Akaba, Malvaacaba, Naminohana: Genus Hosobanamino, Naminohanagosa, Coralthus Genus Ikawanokawa, Genus Kanokawa: Kinokawa, Genus Kaninote: Kaninote, Coralum: Coralum, Genus Mucadenori: Mucadenori, Sujimukade, Catanori, Hiramukade, Kyonohimo, Sakuranori, Nikunukade, Tambanori, Tsurutsuru,
Genus Isonohana: Nuracusa, Chronulaxa, Stinging genus, Hirakintoki Genus: Hirakintoki, Matabou: Matabou, Kintoki: Chabokintoki, Kintoki, Matsunori, Komenori, Tosakamatsu, Aedes pine, Kakureto: Genus Otokinari, Genus genus Genus Nagaobane, Genus Funori: Hanafunori, Fukuronori, Makhunori, Karenikusa Genus: Kalexigusa, Tosakamaki Genus: Hosobanotokamaki, Hirohanotosakamodoroki, Yatsudegatotoka, Kurotosakamodoki, Nezashinokidoka, Nezashinokinusakada Genus: Ezotosaka, Tsukasanori: Enashikarimenia, Otsukasanori, Hanagatakarimenia, Honoo: Honoo, Hikagenoito: Hikagenoito, Usuginu, Nikuhono: Nikuhono Oh, the genus Venice: genus Venice, genus Suskabeni: genus Suskebeni, genus Okamuragusa:
Yamadagusa, Mirin genus: Mirin, Hosobamilin, Tosakanori genus: Kikutosaka, Ezonameshi: Ezonameshi, Isomokka: Isomocca, Yukari: Yukari, Hosoyukari, Ibaranori: Ibaranori, Saidaibara, Tachiibara, Kagiinobaliso, Phyllophorus Isanthus, Atsubanori: Atsubanori,
Ogonori: Ogonori, Tsurushiramo, Shiramo, Ogogonori, Mizokonori, Kabanori, Tengusamodoki: Hachijotengusamodoki, Fushikrenori: Fushikenori, Namiiwatake: Namiiwatake, Spiraea:
Sponge, genus: ssp.
Genus: crocodile, saimi, crocodile, crocus genus: genus genus: moss genus, genus cynosum: squid moss, hosoibonori, nobonori, kurohaginnansou genus: kurohaginnansou, akabaginnansou genus: akabaginnansou, genus genus: genus serrata The genus Thaliosa: The genus Thalassium, Hanasakura, The genus Scrophulari: The genus Scrophulari, Schikonori, The genus Hananoeda: Hanaeda, The genus Hirata: The genus Hirata goat, The genus Darus: The darth, Masagoshibari, Anadarus, The genus Webagusa, Benifukunori
Genus Fusinagi: Fussushinagi, Himefusushinagi, Hirohafushinagisagi, Satsumagiso Genus: Hirawaginagiso, Usubawaginagiso, Igis Genus: Igis, Keigisu, Harigigissu, Haneigisu, Amigusa, Egonomida, Chrysanthemum Genus, Genus genus Family: Habutaenori, Konohanori, Suzuronori, Usveni genus: Usbeni, Hasujiginu: Hasujiginu, Nagakonohanori: Nagakonohanori, Suzuginu: Sujiginu, Atubastinu, Hyasubanori: Kagiusubanori, Jaureusubanoli, Jareusubanoli
Genus Ayanishiki: Genus Ayanishiki, Genus Ayaginu: Genus Ayaginu, Genus Dadia: Enashidazia, Genus Shimadagia: Isohagi, Simadadia, Dazimodoki: Genus Dazimomodoki, Genus Itogusa: Moleutogusa, Genus Genus: Makuri, Yanaginori, Hanayana, Rana Salix var. Genus Isomegusa: Isomegusa, Suzinasugusa: Suzinasugusa, Isobashou Genus: Isobashou, Fujimatsumo Genus: Fujimatsumo, Sawtooth Lilium: Scutellaria Rihiba, genus: genus kawamozuku, okamomozuku, himekamozuku, genus idiomyme: ideyugomegome, genus okitamozuku: genus okitimozuku,
Genus genus, genus chinorimo: chinorimo, genus genus: chisinori).

Axle algae (genus Chara, sp.,
Hossiturimo: Hossiturimo, Lichnotumnus, Flassica: Himefuramo, Chaboflasmo, Tripella, etc., Yellow algae (Hikarimo: Hikarimo etc.) and the like.

Materials derived from animal raw materials include cockscomb extract, bovine / human placenta extract, swine / cattle stomach, duodenum or intestinal extract or its decomposed product, and swine / cattle spleen extract. Products or their decomposed products, extracts of swine or bovine brain tissue, water-soluble collagen, collagen derivatives such as acylated collagen, collagen hydrolysates, elastin, elastin hydrolysates, water-soluble elastin derivatives, keratin and its decomposed products Or a derivative thereof, a silk protein and its degradation product or a derivative thereof, a pig / bovine blood cell protein degradation product (globin peptide), a pig / bovine hemoglobin degradation product (hemin, hematin, heme, protohem, heme iron, etc.), milk , Casein and its decomposition products or their derivatives, skim milk powder and its decomposition products or their derivatives, lactoferrin or its decomposition products, chicken egg ingredients Such as fish meat decomposition products.

(14) Microbial culture metabolites Yeast metabolite, yeast extract, fermented rice extract, fermented rice bran extract, euglena extract, lactic acid fermented product of raw milk and skim milk powder, trehalose or derivatives thereof.

(15) α-hydroxy acid Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(16) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

(17) UV absorbing / blocking agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin B6 derivatives, benzophenones, oxybenzone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, urocanic acid derivatives, escalol, zinc oxide, talc, kaolin and the like.

(18) Whitening agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin B6 derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine and the like.

(19) Tyrosinase activity inhibitor Ascorbic acid and its derivatives, hydroquinone and its glycosides, kojic acid and its derivatives, tocopherol and its derivatives, N-acetyltyrosine and its derivatives,
Glutathione, hydrogen peroxide, zinc peroxide, hydroquinone monobenzyl ether, placental extract, silk extract,
Plant extracts (mulberry, corn, smelt, Clara, mugwort, honeysuckle, yellowfin, dokudami, matsuhodo, adlay barley, edulis, hops, hawthorn, eucalyptus, achillea millefolium, althea, keihi, mankeishi, hamamerisu, yamaguwa, enmeshi, kyoto , Continued spouse,
Sun-dried, mahuang, senkyu, dokkatsu, psycho, bohu, hamabafu, ougon, peony skin, peonies, gennoshoko, kakkon, licorice, quintoko, aloe, ginger, safflower, green tea, black tea, asenyaku).

(20) Melanin dye reduction / decomposition Hydroquinone monobenzyl ether, phenylmercury hexachlorophene, mercuric oxide, mercurous chloride, aqueous hydrogen peroxide, zinc peroxide, etc.

(21) Acceleration of turnover / cell activation Hydroquinone, lactic acid bacteria extract, placenta extract, reishi extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract ( Aloe, ogon, horsetail, gentian, burdock,
Sicon, carrot, hamamelis, hop, yokunin,
Odikoso, Assemblage, Touki, Tokinsenka, Amacha, Hypericum perforatum, Cucumber, Periwinkle,
Rosemary, parsley).

(22) Astringent succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, tannic acid (including catechin compound) Such.

(23) Active oxygen scavenger SOD, catalase, glutathione peroxidase and the like.

(24) Antioxidants ascorbic acid and its salts, stearic acid ester, tocopherol and its ester derivatives, nordihydroguaseletenic acid, butylhydroxytoluene (BHT),
Butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole, propyl gallate, sesamol, sesamolin, gossypol and the like.

(25) Lipid peroxide production inhibitor β-carotene, plant extract (cultured sesame cells, amateur,
Hypericum, Hamamelis, Melissa, Emperor,
Birch, sage, rosemary, Minami Minami, Agetsu,
Ginkgo, green tea).

(26) Anti-inflammatory agent Ictamole, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-
Camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts, licorice extract components, sicon extract, age extract extract and the like.

(27) Antibacterial / bactericidal / disinfectant Acrynol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or its hydrolyzate, alkyl diaminoethyl glycine chloride solution, triclosan, sodium hypochlorite, Chloramine T, salad powder, iodine compound,
Iodoform, sorbic acid or a salt thereof, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, phenol, cresol, p-chlorophenol, p-chloro-m-xylenol, p-chloro-
m-cresol, thymol, phenethyl alcohol, o
-Phenylphenol, Irgasan CH3565, halocarban, hexachlorophen, chlorohexidine, ethanol, methanol, isopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, phenoxyethanol, chlorobutanol, isopropylmethylphenol, nonionic surfactant (polyoxyethylene lauryl) Ether, polyoxyethylene nonylphenyl ether, polyoxyethylene octylphenyl ether, etc.), amphoteric surfactant, anionic surfactant (sodium lauryl sulfate, lauroyl sarcosine potassium, etc.), cationic surfactant (cetyltrimethylammonium bromide, chloride) Benzalkonium, benzethonium chloride, methylrosaniline chloride), formaldehyde, hexamine, Brilliant Green, Malachite Green, Crystal Violet, Germar, Photosensitizer 101, Photosensitizer 201, Photosensitizer 401, N-long-chain acyl basic amino acid derivatives and their acid addition salts, zinc oxide, hinokitiol, quinidine, etc.

(28) Humidifier Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid and its salts, polyethylene glycol, chondroitin sulfate and its salts, water-soluble chitin or chitosan derivatives, pyrrolidone carboxylic acid and its salts, lactic acid Sodium, Mini Sasanishiki extract, etc.

(29) Inhibitors of elastase activity diisopropyl fluorophosphate, plant extracts (Ougon, Hypericum perforatum, Clara, mulberry leaves, calyx, gennoshoko, comfrey, sage, sambucus japonica, bodaiju, buttonpi), seaweed extract and the like.

(30) Hair preparations Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, bromate Sodium, thioglycolic acid, etc.

(31) Antiandrogens Estrogen (eg, estrone, estradiol, ethinylestradiol), isoflavones, oxendrone, etc.

(32) Peripheral vascular blood flow promoter Vitamin E and its derivatives, assembly extract, garlic extract, carrot extract, aloe extract, gentian extract, touki extract, cepharanthin, carpronium chloride, minoxidil and the like.

(33) Local stimulants Pepper tincture, vanylamide nonylate, tincture tincture, tincture tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(34) Metabolic activators Photosensitizer No. 301, hinokitiol, pantothenic acid and derivatives thereof, allantoin, placental extract, biotin, glyceride pentadecanoate, and the like.

(35) Antiseborrheic agents pyridoxine and its derivatives, sulfur, vitamin B6 and the like.

(36) Keratolytic agents Resorcin, salicylic acid, lactic acid and the like.

(37) Oxidizing agent Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, pyrroline peroxide Sodium silicate, sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract and the like.

(38) Hair remover Strontium sulfate, sodium sulfide, barium sulfide,
Inorganic reducing agents such as calcium sulfide; thioglycolic acid or salts thereof (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate, strontium thioglycolate) and the like.

(39) Hair swelling agent Ethanolamine, urea, guanidine and the like.

(40) Dyes 5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-
Amino-4-methylaminoanthraquinone, 3,3'-
Iminodiphenol, 2,4-diaminophenoxyethanol hydrochloride, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, meta-hydrochloride Phenylenediamine, orthoaminophenol, N-phenylparaphenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene
3,4-diamine, nitroparaphenylenediamine, paraaminophenol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol, picramic acid, sodium piclamate, N,
N'-bis (4-aminophenyl) -2,5-diamino-1,4-quinonediimine, 5- (2-hydroxyethylamino) -2-methylphenol, N-phenylparaphenylenediamine, metaaminophenol, meta Phenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate, orthoaminophenol sulfate, orthochloroparaphenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, sulfuric acid Toluene-2,5-
Diamine, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthophenylenediamine sulfate, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol, diphenylamine , Α-naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcin, tannic acid, sodium 2-hydroxy-5-nitro-2 ', 4'-diaminoazobenzozen-5'-sulfonate, hematein and the like.

(41) Flavors Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guayakwood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil, cedar essential oil, spearmint essential oil, western Mint essential oil, Great Fenxia essential oil, Tuberose essential oil, T-shaped essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batturi essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petit glen essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemongrass essential oil, Lemon essential oil, Rosemary Vegetable flavors such as essential oils and Japanese peppermint essential oils, and other synthetic flavors.

(42) Dyes / colorants Red cabbage dye, red rice dye, madder dye, anato dye, squid dye, turmeric dye, ende dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

In addition, hormones, sequestering agents,
pH adjuster, chelating agent, antiseptic / antibiostatic agent, cooling agent, stabilizer, emulsifier, animal / vegetable protein and its decomposed product, animal / vegetable polysaccharide and its decomposed product, animal / vegetable glycoprotein and Decomposed products, anti-inflammatory and anti-allergic agents, wound treatment agents,
Foam thickeners, thickeners, oral agents, deodorants / deodorants, bitters, seasonings, enzymes, etc. are raised.
Various additive and synergistic effects can be expected.

The melanin production inhibitor, active oxygen scavenger, antiallergic agent, histamine release inhibitor, anticaptor active agent and cosmetic composition of the present invention can be in any form, including capsules, powders and granules. , Solids, liquids, gels, air bubbles, emulsions, creams, ointments, sheets, and other pharmaceuticals, quasi-drugs, and skin / hair cosmetics.

Specifically, for example, basic preparations such as topical pharmaceutical preparations, lotions, emulsions, creams, ointments, lotions, oils, packs, etc .; , Pre-show lotion, shaving cream, shampoo, rinse,
Hair treatments, hair styling agents, perm agents, hair tonics, hair dyes, hair growth / hair restorations, etc., makeup cosmetics such as foundations, lipsticks, blushers, eye shadows, eyeliners, mascaras, perfumes, bath agents In addition, it can be applied to various products such as toothpastes, mouth fresheners / gargles, deodorants / deodorants, sanitary cotton, wet tissues, and can be used for general foods and drinks.

The method of adding to the melanin production inhibitor, active oxygen scavenger, antiallergic agent, histamine release inhibitor, anticaptor active agent and cosmetic composition of the present invention is described below.
It may be added in advance or may be added during the production, and may be appropriately selected in consideration of workability.

[0091]

EXAMPLES The present invention will be described below with reference to production examples, test examples, and prescription examples, but the present invention is not limited to these examples.

(Production Example 1) 100 g of each of the plants of linden, shobu, tsuldokudami, and clove was purified water (about 100 g).
(80 ° C) for about 5 hours, followed by extraction, followed by filtration to obtain about 1.0 kg of an extract (dry solid content: about 0.3 to 2.5% by weight).

(Preparation Example 2) 100 g of each of the plants of linden, shobu, tsuldokudami, and clove was immersed in a 50% ethanol solution, extracted at room temperature for 5 days and nights, and then filtered to obtain an extract (dry solid content: approx. 0.3 to 2.5% by weight)
obtain.

(Production Example 3) 100 g of each of the plants of linden, shobu, tsuldokudami, and clove was 70% ethanol solution or 30% 1,3-butylene glycol solution, or 60 g, respectively.
% Propylene glycol solution or purified water (about 30 ° C.), extract at room temperature for 5 days and nights, and then filter to obtain about 1.0 kg of an extract (dry solid content: about 0.3 to 3.0% by weight).

(Test 1) Melanin production inhibitory effect test The present inventors evaluated melanin production inhibitory activity based on B16 melanoma cells.

[Test Method and Evaluation Method] B16 melanoma cells pre-cultured for 24 hours are transferred to a fresh medium, and 0.5% of a sample is added thereto and cultured for 3 days. Next, the cultured cells are treated with trypsin, dissolved by heating in a 1N-NaOH, 10% DMSO solution, and the absorbance at 420 nm is measured. In addition, instead of the sample, distilled water was used as a blank, and cells to which the sample was added at the same time were subjected to an MTT reduction method (Tim Mosmann; Journal).
al. of Immunological Methods p55-63 (1983)), and the MTT reduction activity of the sample-loaded cells was calculated using the MTT reduction activity of blank cells as one unit.
The amount of melanin in the sample was calculated as absorbance / MTT reduction activity (1 unit), and the inhibition rate (%) of the blank cells against the amount of melanin production was determined by <Equation 1>. The result is shown in FIG.
It was shown to. "Sample" As a sample for the melanin production inhibitory action test, the extract (solid content concentration: adjusted to 0.5%) obtained in Production Example 2 of the present invention was used for the test. As a comparative sample, arbutin (manufactured by Sigma) was prepared under the same conditions and used for the test.

<Equation 1> Melanin production inhibition rate (%) = ｛1- (melanin amount of sample /
Blank melanin amount)｝ × 100

(Test Results) As shown in FIG. 1, the plant extracts of the linden, shobu, tsuldokudami, and clove of the present invention can significantly suppress the production of melanin pigment and have a high melanin production inhibitory action. confirmed. or,
Compared with arbutin, it has been confirmed that it has almost the same or higher melanin production inhibitory action, and can be used as a whitening agent.

(Test 2) Elimination of active oxygen (SOD-like activity)
Tests In recent years, many diseases and diseases induced by active oxygen have been reported, and it has been pointed out that removal / elimination of active oxygen (SOD activity) leads to prevention of tissue damage. In this test, the extract obtained in Production Example 2 was tested for its ability to eliminate active oxygen generated by the xanthine-xanthine oxidase system using the NBT method of the Imana et al. (Co., Ltd.).

"Test Method and Evaluation Method" a. After distilling off the solvent from the sample extract under reduced pressure, the solid content concentration in purified water
It was redissolved to a concentration of 0.1% by weight and used for the test. b. Measurement of scavenging ability of active oxygen 0.05M sodium carbonate buffer (pH 10.2) 2.5
mL, 0.75 mM nitro blue tetrazolium (NBT), 3.
0 mM xanthine, 3.0 mM disodium ethylenediaminetetraacetate, 0.15 W / V% bovine serum albumin, and 0.1 mL each of a sample solution (the solvent for the control) were added, and kept at 25 ° C. for 10 minutes.
0.1 mL of an aqueous solution of xanthine oxidase (adjusted so that the change in absorbance of NBT in the control is about 0.3 / 20 minutes) was added, and the absorbance at 560 nm was measured. In addition, a blank containing a reaction stop solution before adding xanthine oxidase was set for each sample and control before adding xanthine oxidase, and the active oxygen scavenging rate was determined by the following formula <Equation 2>. I asked. The results are shown in FIG.

<Equation 2> Active oxygen scavenging rate (%) = {1− (sample OD value−flank (sample) OD value) / (control OD value−flank (control) OD value)} ×
100

(Test Results) As shown in FIG. 2, it was confirmed that each of the plant extracts of the present invention, such as linden, shobu, tsuldokudami, and clove, has an excellent active oxygen scavenging effect.

(Test 3) Histamine Release Inhibition Test In the immediate allergic reaction, histamine and other chemical mediators are released from mast cells or basophils sensitized in the second stage. Therefore, a substance that suppresses histamine release can be expected to have an antiallergic effect. In this test, histamine releasing reagent co
Test method for releasing histamine with mpound48 / 80 (J. Soc. C
osmet.Japan, Vol.25, No.4, P.246 (1992))
The extract obtained in Production Example 2 was examined.

(Test Method) a. Sample The sample for the histamine release inhibition test is Production Example 2 of the present invention.
The extract obtained in was adjusted to a solid content concentration shown below with purified water, and subjected to a test. Solid wood concentration, 0.5% by weight (Fig. 1)
Adjusted. The same concentrations of dipotassium glycyrrhizinate and cromolyn were used as positive controls. b. Measurement of the amount of free histamine A 0.2 mL sample was added to 1.2 mL of mast cell suspension collected from the intraperitoneal cavity of a rat (Slc: Wister male rat, about 4 to 9 weeks old).
And compound48 / 80 were added to a final concentration of 1 μg / mL, and incubated at 37 ° C. for 15 minutes. After stopping the reaction by cooling on ice, the reaction solution was centrifuged, the histamine released from the supernatant was extracted and purified, and the color was developed with o-phthaldialdehyde. The histamine release inhibition rate was calculated by the following formula <Equation 3>, and the results are shown in FIG.

<Equation 3> Histamine release inhibition rate (%) = ｛1- (AC) / (B
-C)｝ × 100 A: Fluorescence intensity of histamine released when a histamine releasing agent was added in the presence of a sample in mast cells B: Fluorescence intensity of histamine released when a histamine releasing agent was added to mast cells C : Fluorescence intensity of histamine naturally released from mast cells (A, B and C are measured values minus blind values)

(Test Results) As shown in FIG. 3, it was confirmed that the plant extracts of linden, shobu, turdkudami, and clove of the present invention have a higher histamine release inhibitory effect than dipotassium glycyrrhizinate or cromolyn. It turns out that it can be used as an antiallergic agent.

(Test 4) Anti-Complement Activity Test The complement system plays an important role in immediate allergy involving antibodies other than the IgE antibody. Substances that affect the complement system include allergies,
It may affect image formation such as inflammation. In this test, the extract obtained in Production Example 2 was examined using an anti-complement activity measurement method using the hemolytic reaction of sensitized red blood cells as an index. (Test method) a. Gelatin / veronal buffer solution (GVB ²⁺ ) 1.7 g of sodium chloride, 0.115 g of barbital, 0.075 g of sodium barbital, 0.015 g of calcium chloride, 0.010 g of magnesium chloride, 0.2 g of gelatin, and 100 mL of purified water are adjusted to pH 7.5. Thereafter, the total amount was adjusted to 200 mL with purified water. b. Sheep red blood cell (SRBC) suspension Sheep blood is centrifuged at 2,000 rpm for 5 minutes, washed three times with physiological saline, and GVB ²⁺ is added to the sediment to prepare a 10% SRBC suspension, which is finally SRBC 3.05 to 0.25 mL of suspension
When complete hemolysis was performed by adding 0.1 mL of a 0.1% sodium carbonate solution, the absorbance at 540 nm was adjusted to 0.455. c. Anti-SRBC Mouse Serum 0.2 mL of 10% SRBC suspension was injected intravenously into the tail of IVCS male mice, 4 days later, blood was collected, serum was separated and GVB
^It was diluted 40 times with ²⁺ and used. d. Complement Guinea pig fresh serum was diluted 20-fold with GVB ²⁺ and used. e. After distilling off the solvent from the sample extract under reduced pressure, the sample extract was redissolved in purified water so as to have a solid content concentration of 0.1 W / V%, and used for the test. still,
The same concentration of dipotassium glycyrrhizinate was used as a positive control. f. Measurement of anti-complement activity 0.1 mL of sample in 0.5 mL of GVB ²⁺ and 0.5 mL of anti-SRBC serum, S
After 0.25 mL of the RBC suspension and 0.25 mL of the complement solution were sequentially added, the reaction was carried out in a thermostat at 37 ° C. for 60 minutes. 10 in ice water
After leaving the reaction to stand for
After centrifugation for 0 minutes to separate unlysed red blood cells, the OD value of the supernatant at 540 nm was measured. A sample containing purified water instead of a sample was used as a control, a blank containing no serum was set for each sample and control, and the complement activity inhibition rate (= anti-complement activity) was calculated by the following equation I asked. The results are shown in FIG.

<Equation 4> Complement activity inhibition rate (%) = {1− (sample OD value−flank (sample) OD value) / (control OD value−flank (control) OD)
Value) x 100

(Test Results) As shown in FIG. 4, the plant extracts of the linden, shobu, turdkudami, and clove of the present invention have excellent anti-complement activity compared to dipotassium glycyrrhizinate. confirmed.

(Test 5) Safety test (1) Primary skin irritation test Various plant extracts obtained in Production Examples 1 to 3 were added to purified water so that the dry solid concentration was about 0.5 W / V%. It was applied to the skin of a Japanese white rabbit (female, 3 per group, weighing about 2.3 kg) whose back was shaved. The judgment was made 24, 48, and 72 hours after application by the primary irritation scoring method using erythema and edema as indices. The result was negative in all animals without any erythema and edema.

(Test 5) Safety test (2) Skin cumulative irritation test Similarly, various plant extracts obtained in Production Examples 1 to 3 were purified to a dry solid content of about 0.5 W / V%. Prepared in water,
0.5m, once a day, 5 times a week, on the skin of Hartley-type guinea pigs (female, 3 per group, weighing about 320g) with shaved flank
L / animal was applied. Application was performed over two weeks and shaving was performed on the last application day of each week. Judgment was made on the day of each application and on the day after the last application using the primary irritancy scoring method using erythema and edema as indices. The result, in all animals,
No erythema and edema were observed over a period of 2 weeks, and the sample was determined to be negative.

(Test 6) Safety test (3) Acute toxicity test Similarly, powders obtained by concentrating and drying the various plant extracts obtained in Production Examples 1 to 3 (dry solid content: about 1.0 W / V%) was orally administered to ddy mice (male and female, 5 mice per group, 5 weeks old), which had been fasted for 4 hours before the test, at a dose of 2,000 mg / kg.
The appearance and degree of toxicity symptoms were observed over time. As a result, no abnormality was observed in all mice for 14 days,
In addition, there was no abnormality in the dissection result. Therefore, LD50 is 2,00
It was determined to be 0 mg / kg or more.

(Prescription Examples) Production of melanin production inhibitor, active oxygen scavenger, antiallergic agent, histamine release inhibitor, anticaptive active agent, and cosmetic composition. As shown, each formulation example may be one manufactured by a conventional method in the manufacture of each product, and only the compounding amount is shown. Further, the present invention is not limited to these.

(Formulation Example 1) Emulsion weight% 1. Squalane 8.0 2. Jojoba oil 7.0 3. Cetyl alcohol 1.5 4. Glycerin monostearate 2.0 5. Polyoxyethylene (20) cetyl ether 3.0 6. Polyoxyethylene (20 ) Soorbitan monooleate 2.0 7.1,3-butylene glycol 1.0 8. Glycerin 2.0 9. A: 50% ethanol extract from Bleach B: 50% ethanol extract from Shoub C: 50% ethanol extract from Turdokudami D: 50% ethanol Extract * Any one of A to D 5.0 10. Appropriate amount of fragrance and preservatives 11. Residual water with 100 purified water

(Formulation Example 2) Peel-off pack wt% 1. Glycerin 5.0 2. Propylene glycol 4.0 3. Polyvinyl alcohol 15.0 4. Ethanol 8.0 5. Polyoxyethylene glycol 1.0 6. Hyaluronic acid solution 1.0 7. Extraction of 50% ethanol Liquid 3.0 8. Clove 50% ethanol extract 1.0 9. Appropriate amount of fragrance and preservative 10. Purified water 100

(Formulation Example 3) Cold cream weight% 1. Salicone beeswax 11.0 2. Liquid paraffin 22.0 3. Lanolin 10.0 4. Almond oil 15.0 5. Olive extract 1.0 6. Borax 0.5 7. Kinoki 50% 1,3- Butyrene recall extract 2.0 8. Shobu 50% ethanol extract 2.0 9. Appropriate amount of fragrance and preservative 10. Purified water 100 residue

(Formulation Example 4) Body soap weight% 1. Potassium laurate 15.0 2. Potassium myristate 5.0 3. Propylene glycol 5.0 4. Clove 70% ethanol extract 0.5 5. Shobu 50% 1,3-butylene glycol extract Liquid 0.5 6. Sekisho 1,3-butylene glycol extract 0.5 7. Carboxymethyl chitin solution 0.5 8. Lemon extract 0.5 9. pH adjuster qs 10. Preservative (methyl benzoate) qs 11. Purified water 100

(Formulation Example 5) Shampoo wt% 1. Triethanolamine lauryl sulfate 5.0 2. Sodium polyoxyethylene lauryl ether sulfate 12.0 3.1,3-butylene glycol 4.0 4. Diethanolamide laurate 2.0 5. Disodium edetate 0.1 6. Extract 30% Fluoro-Penolicol Extract 1.0 7. Clove 80% Ethanol Extract 1.0 8. Royal Jelly Extract 1.0 9. Appropriate amount of fragrance and preservative 10. Purified water 100 residue

(Formulation Example 6) Rinse weight% 1. Stearyltrimethylammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Equine 50% ethanol extract 1.0 6. Water solubility Collagen solution 1.0 7. Hydrolyzed silk solution 1.0 8. Arnica extract 0.5 9. Preservative appropriate amount 10. Purified water 100 residue

(Formulation Example 7) Hair liquid weight% 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Clove hot water extract 1.0 6. Eucalyptus Extract 1.0 7. Seaweed extract 1.0 8. Enamel extract 1.0 9. Preservatives appropriate amount 10. Purified water 100 residue

(Formulation Example 8) Hair tonic% by weight 1. Ethanol 40.0 2. Ethyl oleate 1.0 3. Polyoxyethylene (40) hydrogenated castor oil 2.0 4. Turdkudami 50% 1,3-butylene glycol extract 1.0 5. Clove 30% ethanol extract 1.0 6. Buttonpi extract 1.0 7. Seaweed extract 1.0 8. Comfrey extract 1.0 9. Hydrolyzed elastin solution 1.0 10. Mucopolysaccharide solution 1.0 11. Appropriate perfume / preservative 12. Purified water 100 Residue to do

(Formulation Example 9) Granule bath agent weight% 1. Sodium hydrogencarbonate 58.0 2. Anhydrous sodium sulfate 30.0 3. Borax 3.0 4. Equine 70% ethanol extract 5.0 5. Chimp extract 2.0 6. Tochu leaf extract 2.0

(Formulation Example 10) Coating protective agent A gauze or liniment cloth was coated with a 30% ethanol extract of Shoub.
It is impregnated with a 30% ethanol extract, a prescription solution containing appropriate amounts of antibiotics and anti-inflammation, and attached to the wound. or,
The Shobu 30% ethanol extract and the Shoub 30% ethanol extract may be directly sprayed locally and covered with gauze or the like.

(Test 7) Use effect test The effect when the cosmetic composition of the present invention was actually used was examined. The use test is a panel of 10 people aged 30 to 60 years old who are worried about spots, freckles, dark and dark, and those who are worried about dry skin and no shiny skin. This was done by applying an appropriate amount of the emulsion of Example 1 to the face for 3 months. As a control, a mixture prepared by removing the respective plant extracts of the present invention from the emulsion in the same manner was used. The evaluation was performed according to the following criteria. The results are as shown in <Table 1> and <Table 2>, and the numerical values in the table represent the number of persons. No one complained of abnormal skin during the use period.

"Stain, freckles and skin color improving effect" Effective: The freckles, freckles and skin color were white and reduced. Slightly effective: White spots and freckles and skin color were slightly reduced. Ineffective: No change from before use.

[0126]

"Effect of improving skin feel" Efficacy: Dry skin and skin gloss / tension increased, and skin was improved. Slightly effective: Dry skin and skin gloss / tension increased slightly, and skin was improved. Ineffective: No change from before use.

[0128]

(Test Results) As shown in Tables 1 and 2, the emulsion containing the plant extracts of the linden, shobu, tsuldokudami, and clove of the present invention significantly improved dry skin and gave glossy skin. It was confirmed that the skin was whitened and the spots, freckles and skin color were white and reduced.

(Test 8) Use Effect Test The effect when the skin care cosmetic composition of the present invention was actually used was examined. Use test is for those who suffer from rough skin,
The test was performed by applying an appropriate amount of the emulsion of Formulation Example 1 to the face over three months, twice a day, in the morning and at night, after washing the face twice a day, with 10 persons aged 30 to 50 as panelists. As a control, a mixture prepared by removing the respective plant extracts of the present invention from the emulsion in the same manner was used. The evaluation was performed according to the following criteria. The results are as shown in Table 3 and the numbers in the table represent the number of persons. No one complained of abnormal skin during the use period.

"Beautiful skin effect" Effective: Skin roughness was improved. Somewhat effective: skin roughness was slightly improved. Ineffective: No change from before use.

[0132]

(Test Results) As shown in Table 3, it was confirmed that each of the plant extracts of the present invention, such as linden, shobu, tsuldokudami, and clove, improved skin roughness.

Industrial Applicability The melanin production inhibitor, active oxygen scavenger, antiallergic agent, histamine release inhibitor, anticaptor which contains at least one plant extract selected from linden, shobu, tsuldokudami and clove of the present invention The active agent and the cosmetic composition have an excellent melanin production inhibitory action, a whitening effect is also confirmed, and also active oxygen is significantly eliminated / suppressed, further, histamine release inhibitory action, anticomplement activity It was confirmed. And to reduce stains and freckles on the skin. In addition, skin inflammatory diseases (eg, redness, edema,
Eczema, etc.) ・ Improvement of rough skin and dry skin.
It can also provide tension and is safe for humans and animals. The present invention can be used for other general foods and drinks.

[Brief description of the drawings]

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1.
It is a figure which shows the suppression rate with respect to the melanin production amount of each plant extract of a butterfly.

FIG. 2 shows the linden, show, turdkudami,
It is a figure which shows the active oxygen scavenging effect of each plant extract of Clove.

FIG. 3 shows a linden, a show, a turdkudami of the present invention.
It is a figure which shows the histamine release inhibitory effect of each plant extract of a butterfly.

FIG. 4 shows a linden, a show, a turdkudami of the present invention;
It is a figure which shows the capturing activity inhibitory effect of each plant extract of a butterfly.

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61P 37/08 A61K 31/00 637E 37/06 637D 39/06 639C 43/00 643F F-term (Reference) 4C083 AA082 AA111 AA112 AA122 AB152 AB312 AB352 AC022 AC072 AC122 AC182 AC242 AC342 AC392 AC432 AC442 AC542 AC782 AC902 AD042 AD112 AD312 AD332 AD432 CC05 CC07 CC23 CC32 CC38 DD27 DD32 DD33 EE13 EE42 4C088 AB14 AB43 AB57 AB09 AC03 CA07 AC06 ZA89 ZB01 ZB13 ZC13 ZC41

Claims (6)

[Claims] 1. A melanin production inhibitor characterized by containing at least one plant extract selected from linden, shobu, tsukudokudami, and clove. 2. An active oxygen scavenger comprising at least one plant extract selected from linden, shobu, tsukudokudami, and clove. 3. An anti-allergic agent comprising at least one plant extract selected from linden, shobu, tsukudokudami and clove. 4. A histamine release inhibitor comprising at least one plant extract selected from linden, shobu, tsukudokudami, and clove. 5. An anti-complement activator comprising at least one plant extract selected from linden, shobu, tsukudokudami, and clove. 6. It contains any one or more of the melanin production inhibitors, active oxygen scavengers, antiallergic agents, histamine release inhibitors and anticaptor active agents according to claims 1 to 5. A cosmetic composition comprising: