JP4947034B2 - Polylysine preparation and cosmetic composition containing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide new antiallergic agents and histamine isolation inhibitors, and a safe cosmetic composition containing at least one of them. <P>SOLUTION: The antiallergic agents contain &epsi;-polylysine. The histamine isolation inhibitors contain &epsi;-polylysine. The safe cosmetic composition contains at least one of them. The &epsi;-polylysine is allowed to use free &epsi;-polylysine; an inorganic salt of &epsi;-polylysine formed by &epsi;-polylysine with such inorganic acids as hydrochloric acid, sulfuric acid and phosphoric acid; and an organic salt of &epsi;-polylysine formed by &epsi;-polylysine with such organic salts as acetic acid, propionic acid, fumaric acid, malic acid, citric acid, maleic acid, adipic acid, gluconic acid and lactic acid. The cosmetic composition improves eczema, pimples and skin roughness; gives gloss, firmness and moistness to the skin; and is safe for humans and animals. <P>COPYRIGHT: (C)2009,JPO&amp;INPIT

Description

  The present invention relates to an antiallergic agent containing ε-polylysine and a histamine release inhibitor containing ε-polylysine (sometimes collectively referred to as a polylysine preparation) and a cosmetic composition containing the preparation.

  The polylysine preparation of the present invention and the cosmetic composition containing the preparation can be used, for example, in various external preparations (including preparations used for animals) in general, specifically, ampoules, capsules, pills. , Tablets, powders, granules, solids, liquids, gels or bubbles 1) Pharmaceuticals, 2) Quasi-drugs, 3) Topical or systemic skin cosmetics (eg lotions, emulsions, creams, ointments) , Basic cosmetics such as lotions, oils, packs, facial cleansers and skin cleansers, massage agents, cleansing agents, depilators, depilators, shaving treatments, after-shave lotions, pre-show lotions, shaving creams, foundations Makeup cosmetics such as lipstick, blusher, eye shadow, eyeliner, mascara, perfume, nail polish, nail enamel, nail enamel remover, poultice Plasters, tapes, sheets, patches, aerosols, etc.), 4) medicinal and / or cosmetic preparations (for example, shampoos, rinses, treatments, permanent solutions, dyes) Hair, hair styling, hair styling agent, hair growth and hair nourishing agent, poultice, plaster, tape, sheet, patch, aerosol, etc.), 5) bathing agent used in bath water, 6) other, It can be used as a foul odor preventing agent, deodorant, deodorant, sanitary article, sanitary cotton, wet tissue, mouth freshener (for example, toothpaste, mouthwash, etc.).

  Allergic diseases such as hay fever, allergic rhinitis, bronchial asthma, atopic dermatitis, drug urticaria, etc. due to recent deterioration of environmental conditions, changes in lifestyle, and increased stress accompanying social life complexity An increasing number of people suffer from rough skin, dry skin, and the like. In particular, atopic dermatitis has been attracting attention as one of the modern diseases because its increase has been screamed in a wide range of ages ranging from children to adults.

  Originally, in order to eliminate foreign substances (bacteria, pollen, ticks, etc .: antigen) that have entered the body, the immune system that works to protect the body by producing biological components (antibodies, lymphocytes, etc.) against it It has a function. However, sometimes the reaction becomes so sensitive that it can be harmful to the body and cause various diseases. This disordered response due to immune function is called allergy and is classified into four types.

  The central cell constituting the immune system is a group of two types of lymphocytes called B cells and T cells that exist mainly in bone marrow, thymus, spleen, lymph nodes, blood and the like. B cells are involved in humoral immunity (types I to III allergy), and in response to antigens, differentiate and proliferate into plasma cells (antibody producing cells), and secrete antibodies outside the cells. T cells are mainly involved in cellular immunity (type IV allergy). When they react with antigens, they become activated T cells that differentiate, proliferate, and destroy antigens.

  Type I allergic reactions are the most frequently occurring type, and mainly involve immunoglobulin E (IgE) antibodies. The onset process is the first stage in which IgE antibody is produced from B cells against foreign antigens, and IgE antibody adheres to mast cells and basophils. The second stage in which chemical mediators such as histamine, serotonin, and SRS-A are released from the body, the released chemical mediator causes smooth muscle contraction, increased vascular permeability, edema, nerve stimulation, etc. It is roughly divided into the third stage in which allergic symptoms are manifested.

  Type II allergic reaction is a disordered reaction caused by destruction of tissue cells by IgG and IgM antibodies. When an antibody binds to an antigen cell, the complement system (a system of serum proteins that is activated by the effector action of the antibody and kills the antigen cell or significantly accelerates the degradation of the antigen by phagocytes) is linked and activated. The cells are destroyed because a complex that breaks the cell membrane is formed. In addition, when activated components of the complement system bind to cells, antigen cells are captured, decomposed, and removed by phagocytic cells such as polymorphonuclear leukocytes and macrophages. On the other hand, in the process of activation of the complement system, a substance called anaphylatoxin having an action of remarkably accelerating the degradation of antigens by phagocytes is generated. This also has the effect of increasing the permeability of capillaries, the effect of contracting smooth muscles, and the effect of releasing histamine from mast cells. Therefore, if it is produced in excess, various allergic symptoms will appear.

  Type III allergic reactions are mainly caused by IgG antibodies, but their onset occurs by a complex mechanism involving many factors such as the complement system and polymorphonuclear leukocytes. Antigen-antibody conjugates produced by binding antibodies to antigens are deposited in tissues such as blood vessels, kidneys, joints, and skin to activate the complement system. When the amount of conjugate is high, the complement system is over-activated to produce anaphylatoxins, which increases inflammation and causes inflammation. In addition, when phagocytic cells work to remove antigen-antibody conjugates due to the action of anaphylatoxin, lysosomal degranulation occurs, and many types of hydrolases such as protease in the lysosome are released outside the cell. Tissue and cell damage progresses and type III allergy develops.

  All types I to III allergic reactions are due to humoral immunity, appearing in 2 to 3 minutes after contact with the antigen, and the reaction is highest in 10 minutes, so it is called immediate type, whereas type IV Allergic reactions appear after a few hours and are called delayed allergies because it takes 48-72 hours to reach a maximum. This allergic reaction is a reaction due to cellular immunity. A part of T cells reacts with an antigen to become activated T cells. When this reacts with the antigen again, various inflammatory factors (macrophage chemotactic factors, Lymphocyte migration factor, macrophage activation factor, vascular permeability factor, etc.). These work to eliminate antigens, but if this reaction proceeds excessively, allergies develop as a disordered reaction.

  Various test methods have been proposed according to the allergic reaction types classified as described above, and approaches to factors effective in preventing or ameliorating allergic diseases have been actively conducted so far.

  Many of the antiallergic agents that have been used in the past are drugs related to diseases caused by immediate allergic reactions whose action points are relatively clear. For example, for type I allergies, antispasmodics that relax smooth muscles, sympathomimetic drugs that suppress increased permeability of capillaries, and antihistamines, etc., are all listed. It is a drug that acts on the stage and is effective as a symptomatic treatment, but most of them are synthetic drugs and have problems in terms of side effects. Furthermore, although research is actively conducted on the development of drugs effective for type II to type III allergies, no specific antiallergic agent has yet been found.

In view of these circumstances, the present inventors have intensively studied to develop useful substances having antiallergic action and histamine release inhibiting action. As a result, it was found that ε-polylysine has a histamine release inhibitory action. It has also been found to be a highly safe antiallergic agent and histamine release inhibitor that can improve eczema, acne, rough skin, and also give skin gloss, tension and moisture, and one or more of these. It has been found that a cosmetic composition containing the above becomes a cosmetic having these effects.
That is, the present invention is an antiallergic agent and histamine release inhibitor containing ε-polylysine, and a cosmetic composition containing one or more of these.

  The polylysine preparation and cosmetic composition of the present invention have a histamine release inhibitory action, can improve eczema, acne and rough skin, and can give skin gloss, tension and moisture to humans and animals. It is also safe. The polylysine preparation of the present invention can be used for other general foods and drinks.

  The ε-polylysine used in the present invention may be obtained by any method. Specifically, the Streptomyces alpras sub-species lysinopolymeras described in Japanese Patent No. 1245361 is converted into glucose. 5% by weight, yeast extract 0.5% by weight, ammonium sulfate 1% by weight, dipotassium hydrogen phosphate 0.08% by weight, potassium dihydrogen phosphate 0.136% by weight, magnesium sulfate heptahydrate 0.05% by weight , Zinc sulfate heptahydrate 0.00 wt. 4%, iron sulfate heptahydrate 0.03% wt., Cultured in medium adjusted to pH 6.8, and separated and collected from the resulting culture Ε-polylysine obtained by doing so.

  In the present invention, free ε-polylysine may be used, and an inorganic acid salt of ε-polylysine formed from ε-polylysine such as hydrochloric acid, sulfuric acid, and phosphoric acid and acetic acid, propionic acid, fumaric acid. Organic acid salts of ε-polylysine formed from organic acids such as acid, malic acid, citric acid, maleic acid, adipic acid, gluconic acid and lactic acid and ε-polylysine, caproic acid, lauric acid, stearic acid, etc. Saturated fatty acid salts of ε-polylysine formed from medium and long chain saturated fatty acids and ε-polylysine, medium and long chain unsaturated fatty acids such as oleic acid, linoleic acid, and arachidonic acid and ε-polylysine An unsaturated fatty acid salt of ε-polylysine formed by the above may be used, or a mixture thereof.

In addition to polylysine, α-polylysine produced by a synthetic method can be used, but for this purpose, ε-polylysine is preferable. The toxicity of ε-polylysine used in the present invention is very low. For example, the acute toxicity of ε-polylysine hydrochloride in oral administration to mice is 5 g / kg or more at LD ₅₀ . Further, ε-polylysine may be prepared by arbitrarily drying, concentrating, or diluting depending on the dosage form / form of the polylysine preparation or cosmetic composition.

  When the polylysine preparation of the present invention and the cosmetic composition containing the polylysine preparation are used in combination with an electrolyte having a pH buffering ability and an amino acid, an excellent synergistic effect is observed. Examples of electrolytes having pH buffering capacity include phosphoric acid-phosphate metal salt, acetic acid-acetic acid metal salt, lactic acid-lactic acid metal salt, succinic acid-succinic acid metal salt solution, tartaric acid-tartaric acid metal salt, citric acid-citrate metal Salt, malic acid-malic acid metal salt, and the like. Further, the type of metal salt is not particularly limited, but sodium salt or potassium salt is preferable from the viewpoint of solubility. The content of the electrolyte having a pH buffering capacity is 0.001 to 20% by weight, preferably 0.01 to 10% by weight, based on the total amount of the polylysine preparation or the cosmetic composition. If the content is less than 0.001% by weight, sufficient pH buffering ability may not be exhibited, and if it is more than 20% by weight, it is economically disadvantageous.

  The pH of the polylysine preparation or cosmetic composition of the present invention varies slightly depending on the type, quality, and expected action, but is adjusted to a range of 3.0 to 9.0, more preferably 4.5 to 6.5. It is preferable to do. When the pH is less than 3.0 or 9.0 or more, there is a case where irritation is felt on the skin during use, which is not preferable. Such pH adjustment can be obtained by appropriately selecting the composition of the electrolyte.

  Examples of amino acids used in combination include neutral amino acids (glycine, alanine, valine, leucine, etc.), acidic amino acids (aspartic acid, glutamic acid, etc.), basic amino acids (lysine, arginine, etc.), etc. -Most preferred is the use of glycine or alanine, where a significant synergistic effect is observed in combination with polylysine. The content of these amino acids is 0.001 to 25% by weight, preferably 0.01 to 20% by weight, based on the total amount of the polylysine preparation or cosmetic composition. If the content is less than 0.001% by weight, a significant synergistic effect with ε-polylysine may not be observed, and if it is more than 25% by weight, it is economically disadvantageous.

  The content of ε-polylysine in the polylysine preparation or cosmetic composition of the present invention is not particularly limited, but the type, quality, expected antiallergic action and histamine of the polylysine preparation or cosmetic composition are not particularly limited. It is slightly different depending on the degree of the release inhibiting action, and is usually 0.001% by weight or more (hereinafter simply expressed as%), preferably 0.01 to 5%. If the content is less than 0.001%, the effect cannot be expected sufficiently.

  In addition, the polylysine preparation or cosmetic composition of the present invention includes, in addition to the electrolyte having the pH buffering ability and the amino acid, the components exemplified below as long as the effects of the present invention are not impaired, if necessary. Can be manufactured by arbitrarily selecting and using additives, and the content in the polylysine preparation or cosmetic composition can be any anti-allergic agent or histamine release inhibitor polylysine preparation or cosmetic composition. Depending on whether or not, usually 0.0001 to 50% is preferable.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, sesame oil, olive oil, orange oil, orange rafa oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, beef tallow fatty acid, cucumber oil, safflower oil , Shea fat, liquid shea fat, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, owl, palm Oil, beef tallow, pork tallow or hydrogenated products (hardened oil etc.) of these fats.

(2) Wax beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, rice wax, squalene, squalane, pristane, etc.

(3) Mineral oils Liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcrystalline wax, etc.

(4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid, 2- Synthetic fatty acids such as ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.

(5) Alcohols Natural alcohols such as ethanol, isopyropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol and phytosterol, and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol and 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, myristyl lactate, Diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) gum, sugar or water-soluble polymer compound gum arabic, benzoin rubber, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, lactose, fructose, sucrose or ester thereof, Trehalose or derivatives thereof, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) chitin or chitosan to which alkylene (C2-C4) oxide such as ethylene oxide is added, low molecular chitin Or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or salt thereof, hyaluronic acid or salt thereof, chondroitin sulfate or salt thereof, heparin, ethylcellulose, methylcellulose Polycarboxylate such as carboxymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyethylene oxide and polypropylene oxide Alkylene oxide or its crosslinked polymer, carboxyvinyl polymer, polyethyleneimine and the like.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate ester salt, alkyl phosphate ester salt), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt), amphoteric Surfactant: Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant ( Ether type nonionic surfactant, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants ( Natural surfactants, protein hydrolyzate derivatives, polymer surfactants, surfactants containing titanium and silicon, fluorocarbons System surfactant), and the like.

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), Riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C group: vitamin C acid or derivatives thereof, vitamin D group: Ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotaxosterol, vitamin E group: vitamin E or derivatives thereof, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K2), me Dione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, .gamma.-oryzanol, orotic acid, vitamin P such (rutin, eriocitrin, hesperidin), vitamin U and the like.

(12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine , Arginine, ornithine, histidine and the like, and their amino acid derivatives such as sulfate, phosphate, nitrate, citrate or pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These are processed in a conventional manner according to the type and form of the product to be added (for example, grinding, milling, washing, hydrolysis, fermentation, purification) , Compression, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc., arbitrarily selected and combined), arbitrarily selected from various materials You can use it.

  The solvent used for the extraction may be selected in consideration of the intended purpose and type of the product to be provided, or the processing performed later. Usually, water, a water-soluble organic solvent (for example, ethanol, propylene glycol) , 1,3-butylene glycol, etc.) is preferably used. However, when it is not preferable to contain an organic solvent depending on the application, it is sufficient to use only water or ethanol that can be easily removed after extraction, and it may be used alone or in any mixture with water. The thing extracted and extracted may be sufficient.

  In addition, when using additives derived from plant or animal-based ingredients for systemic or topical external preparations and cosmetics, it protects skin and hair, moisturizes, improves touch and texture, imparts flexibility, and stimulates Relaxation, stress relief by fragrance, cell activation (cell aging prevention), suppression of inflammation, improvement of skin and hair quality, prevention of rough skin and its improvement, hair growth, hair growth, hair loss prevention, imparting gloss, cleansing effect, In addition to cosmetic effects such as fatigue relief, blood flow promotion, and warm bath effects, effects such as flavoring, deodorization, thickening, antiseptic, and buffering can also be expected.

  Specific plants (herbal medicines) as raw materials include, for example, almonds (toen), ai (indigo leaves), aokazura (Keifu Fuji), Aoki (Aoki), Aogiri or Kenashia Ogiri (Ao Tung), Aotsu Samurai (wooden candy) , Acacia, red pepper (red urn), red spruce `` fruit '', red clover, red grape, red sardine (logwood), red wrinkle (red bud), red radish (red bud), red radish or red squirrel (yellow), agi (red eel), red pine , Akinire (crust), Aguay Guasu, Aguahe (Omitengu Palm), Akebi (Kitsu), Asa (Makojin), Malva morning glory or morning glory (Toshiba), Hydrangea (hydrangea), Ashitaba (Tomorrow), Azuki (Red Azuki), Acerola, Asenyaku (Asen Yaku), Athira (Shokuyo Kanna), Anise, Abiu, Abiurana, Brassica, Abemaki `` Fruit '', Avocado, Ama, A Macha (Amacha), Achacharu, Amadokoro (Tamatake), Amana (Kojito), Amaranth (Amaranthus, Hagatetou, Higegateto, Senninkoku, Sugimorigateto, Hosooaogeito, Aogateto, Haribiyu, Amaranthus Hippocondrix ), Aristolomeria (Yurizuisen), Arcana (Arcanna), Argarobo (Cabbage), Altea, Arnica, Alpinia or Katsumadai (Suzukuku), Aloe Vera, Aloe Vera, Angelica, Apricot or Honanzu (Anzujin), Ansokkou (benzoic incense), Yellow support, Izokouzolina (Gallhead), Ikarisou or Yachima Taikoshi (Yinokukaku), rushes (Ginseng grass), Japanese knotweed (Tiger root), Yew (first place), Strawberry, Fig (No fruit "fruit, leaf"), Ichihatsu (First), Ichibi (Winter Reiko), Ichi Ginseng (one herb), Ginkgo (Ginkgo `` Seeds, Leaves ''), Japanese beetle (distant), Carob bean, Inazuna (Tailexi), Inubiyu (Honagainubiyu), Rice `` Seeds, Seed coat '', Inondo `` Seeds '', Ibukikakuso, Nettle, Ylang Ylang, Iwa Tobacco (Iwabuchi), Iwahiba or Iwamatsu (Makimaki), Oolong Tea, Fennel (Welcome), Wheat (Chibusanoki), Ukiyagara (Three Ridges), Uguisukagura `` Fruits '', Japanese Meadow, Turmeric (Uranium), Duckweed (Float), Usubasaicin or Keirinsaishin or Oshusaishin (spicy), Usbenia Oy, Usubenitachia, Nepenthes (Summer hay), Udo or Shishiudo (Aki, Aki, Tang Dynasty), Unyadegato (Uncaria, Cats Claw) , Uba tea, Japanese apricot (Japanese apricot `` seed, pulp ''), Vulture, Japanese eel Sea urchin (Chen), Estragon, Ezoukogi (蝦 夷 五 加), Echinashi (Horaxum barrengiku), Enishida, Enokitake (榎 茸), Ebisugusa or Cassia tora (Keiko), El Campre, Elderberry `` fruit '', Elemi, Elingi or Pre Lotus Elinji, Enju

  Ougi or Kibanaougi (Yellow Twilight), Ousei (Naruco Yuri, Kagura Mabanaru Yuri), Ohisano Yuki or Sasano Yuki, Ouren (Yo Ren), Ogata Hakken, Okarasuuri (Karokon), Ooguruma (Civil Scent), Otsu Rafuji Car forerunner, car forerunner), toucan beetle (lion), toucan cactus, toucan okera or okera (white birch), giant beetle (banaba), tossed beetle "fruit", tossed beetle (cereals), tossed hawthorn, hawthorn or hawthorn Mountain barley), Barley (barley), Oka, Okazeri (snake bed), Okra `` fruit '', Oguruma (overturn), Panax ginseng or Tochibaninjin (carrot), Hypericum or Sorbus, Hypericum pertussis (brothered cut grass), Odorikosou (continuous) , Onamomi, Onigurumi, Onidoco Bronze or Tokoro or Nagadokoro (Hikai), Onino Yagara (Tempa), Onyuri or Sasayuri or Hakatayuri (Yuri), Onionis, Halibut (Kiribana), Ominaeshi (losing sauce), Oyuko, Dutch mustard (Cresson), Dutch jelly, Netherlands Honey Bees, Olives “Fruits, Seeds, Leaves”, Oregano, Orange “Fruits, Peels”, Silkworms (Mature Yellow), Cacao “Fruits, Peels, Seeds”, Oysters (柿蒂 “Leaves”), Kagi Kazura ), Kakidooshi or Kantori (Lotus herb), Gaku hydrangea, Cassia, Casino oyster (fruit `` fruit ''), Gadju (莪 朮), Kashiwa (Kashiwagi, Kashiwa), Cascarilla, Cascara grada, Gypsophila, Gypsophila, Crab (Kinshato), Kaniwa, carnation, valerian (valerian), birch or fern birch (white birch), gerbera, pumpkin, kapoknoki "seed", kaho Salamander (蜀椒), Catfish (蒲 yellow), Chamomile or Roman chamomile, Camille deer (weed), Cam cam (camo duck), Collar, Carax salmon man, Prunus or Chinese pear (Wang (), Crow bishak (half-summer), oats, guarana `` Seeds '', Karahoo (Koh-Pak), Karaya, Garyu, Karin (Kiso), Garcinia, Kawamidori, Kawara-Psycho (Koryo-cho, Hakucho), Kawajisha, Kawaratake, Kawaranadeshiko (Ishitake) or Ezokawaranadeko (Soba, Buckwheat) , Chinese carrot (blue potato), Chinese mugwort (green strawberry), Kansui (sweet candy), licorice (licorice), Kantarasa, Kandelira, Kantou, Kanna, raspberry (Ezo strawberry, Dutch strawberry, Shrimp strawberry, Navashiro strawberry, Momiji strawberry, European raspberry) , Kiwi "fruits, leaves", Kikarasuuri (Gurone), Kiko (kikyo, bellflower) ), Chrysanthemum, chrysanthemum, chrysanthemum, chrysanthemum, caterpillar (gumnuts), gypsophila (sheep's root), pheasant (kurami), kizuta, yellow aloe vera, yellowfin mint, quina, linden (cinchona, red linden), quinoa (quinoa) , Yellowfin, gymnema sylvestre, kimenakaku, cabbage, cabbage `` immature fruit '', cucumber, goryo (west river willow, cedar willow), kiraja saponaria, kiraya, ginsau, fruit, ginsekai , Kimbusen, betel, Kimizumi (sentsukakusa),

  Guapeva Vermela, Guava `` fruit '', Guayule, Quercus Infectria (garlic), Cucuin nuts, wolfberry (bamboo shoots, coconut leaves, cocoon leaves, husks), Kusasagikazura (Tenmon winter), Kudzu (Kadoku), Kusunoki, Goose Berry `` fruit '', xanthine carrot (yellow flower moth), cutiti river, gardenia (mountain lion), kunugi (樸 saw), kupuas, kumazasa, kumatsura (horse whip grass), clara (bitter), cranberry `` fruit '', chestnut `` `` Seeds, fruits, astringent skins '', curcrigo latifolia `` fruits '', grapefruits `` fruits and leaves '', buckthorn, black scabbard (emergency), black-faced toads or toads (prolonged life), cloves (clove, clove), gumbai tuna (sekimei, (Sekimeishi), Kei-gai (bamboo shoots, Aoiho), Keito (chicken crown flower, chicken crown), gekkavidin, bay bay (laurel) Kenashi Sartorii rose (earthen soil, mountain return), Gentiana, Gennoshouko (Oldgrass grass), Kemponashi (Kigushi), Kokisekoku, Kushimikan (Koji, Tachibana, Obenimikan, Fuclican, Sagamikouji, Ponkan, Santara (Tachibana bark)), Koshinbara (Monthly flower), kousuihakka, kouzo `` fruit '', kocha (tea), kohone (river bone), kouhon (Tsubakimoto, Karatsumoto), kouryang, koukyokyo (Kora 姜), cilantro `` fruit '', kouren (huoren) , Koganebana (Yellow Gon), Cowberry (Koshitachi), Coco `` Fruit '', Goshuyu (Kurei), Gosho Strawberry (Pepper), Pepper (Pepper), Cosmos, Copaiba Balsam, Coffee `` Seeds, Leaves '', Kobushi or Mokuren (Spicy rice cake), burdock (beef bowl, beef eggplant), kobotan eel, sesame (sesame seeds), sesame seedling (genus ginseng), rubbish (poison), sanekaz La or binankazura or pine bush, wheat (wheat), rice or rice bran `` red cocoon, white birch '', rice oil, cola acminata `` seed '', cola vera `` seed '', colony laja, fenugreek `` fruit '', colombo, condurango, kombu , Konjac, comfrey, sisal (sisal asa), saxima button leopard or cinnabar leopard or cinnamon (wei Sensen), cherry (Oshima cherry, Yamazakura, Oyamazakura, edhigashi, bean cherry, Miyazakura, Yoshino cherry, Takaneza , Kohigan, Satozakura, Kanzakura (leaves, flowers, fruits, bark (cherry bark))), cherries, pomegranates, sasa, sasanqua, sardine squirrel, potato, sugar cane, sugar beet, saffron jujube, saffron Safflower, safflower, sapotea Lillo, pomelo `` fruit '', savonso, salmon berry `` fruit '', salamander (sasma), salvia (sage), scallops (mountain vegetables), sawagurumi (wild walnuts), sankaku cactus, sangre degrad (croton), sansiku Carrot, Sansine carrot (Ginseng ginseng), Sansyuyu (Yamamuro), Sansho (Yamamuro), Sandskon (Yamazune),

  Shia (carite), Cyanoki `` fruit '', shiitake (shiitake), shion (purple candy), jikitarisu, shikunshi (messenger), perilla or ajizo or chimmento or catamendizo (purple leaf, shisoko), rosewood, linden tree, red linden tree, Chinese cypress Forsythia, shimeji (hinshimeji, shakashimeji, hatake shimeji, oshiroi shimeji, beech shimeji, honjimeshi, shirotakemotake), shimokren (hot pepper), shimotsukeso, potato, peonies, shajin (jap), lily (Bakumon Winter), Shukukonkamisou, Shukushamitsu (Sanjin, Shredded Sand), Juzudama, Shuro `` Fruit '', Ginger (Ginger), Gyozan Hydrangea (Tsuneyama), Shobu (Koji, Sone), Shozuk `` Fruit ”, Shirakashi“ Seeds ”, White Rooster“ Seeds ”, White clover (Clover), White toe Ki (chicken bone grass), white squirrels (scented squirrels), Shirobanatsuta "flower", shirominanten (southern tenmami), cinchona sill bra, ginkgo biloba (mika, sika), cinnamon, cassia (cinnamon), watermelon (Saijo), Honeysuckle (Silver flower, Shinobu), Sorrel (Acami), Swimberry `` Fruit '', Star Apple, Stevia, Strawberry `` Fruit '', Suzu-Psycho (Xucho), Sugina (Question), Suburihiyu (Horse tooth soap, (Horse teeth), plum (fruit), Japanese red pine (cone), Japanese larch, Japanese lizard, Japanese walnut, Japanese hawthorn, Japanese dandelion, Japanese horse chestnut (Maroni), Japanese pear `` fruit '', Japanese cherry tree, Japanese elderberry (Elder), Apricot (Juniper, Juniper), Achillea millefol (milfoil) Rose, pearl butterfly, mistletoe, yellow mint or wild mint , Senshinren (Penshinren), Sendan, Sennin cactus, Senna `` fruit, leaf '', Senninsou (Otsuka), Semburi (medicinal herb), Soka (grass), Elephant spinach, Soshishinbai, buckwheat `` seed '', Somer potato, Diou (Daihuang), radish, soybeans, daidai (orange peel, fruit), takasaburo (bamboo lotus), takatodai (daikon), kawarabi (sword spine), dark sweet cherries `` fruit '', hollyhock oyster, ginger mulberry (thyme, perilla) ), Tachidokoro (hikai), Tachiyanagi, Tamarind `` seed '', onion, beetle Bamboo (powder), taranoki `` fruit, leaves, root bark '', dahlia (tenjiku button), tarwi, tanshichiwa, tanjin (dansang), dandelion (Shang Xining) or Shirobana dandelion or mouko dandelion, danmar,

Cherry `` fruit '', chigaya `` fruit, root, bud '', chikutsuninjinjin (bamboo ginseng), chicory, chankapiedora (Kidachikomikansou), ginseng (daihuang), ginseng (bamboo), ginseng (bamboo leaves), Choreimaitake (bamboo shoots), chorogi, camellia, tsukuritake (mushrooms), camellia, camellia, tsumekusa (lacquered vines), camellia (duck vines), tsurumazuki (red azuki beans), tsurudukudami (what neck candy), tsuruna (apricot) , Tsurun carrot (yotsuba ginseng), tsuwabuki, deiko, teuchigurumi, dubery `` fruit '', tulip, choconostay, tengusa, tencha (chapter tea), tendaiyaku (glaze), chili (winter coconut), chief quail, chili pepper (banban) ), Toki (home and going back), Tokin-Senka (Marigold), Tounabena (river cut), corn or corn hair (Southern shark), Mouse mochi (Sadako), Torindou or Kyakusen Gindou (Ryuboku), Tokusa (wood band), Dokudami (10 medicines), Tokon (Togashi root), Toshishi or Mamedaoshi or Nekazura, Tochu (Tochu `` bark, leaf, root ''), Ash (crust), tomato, tragacanth, triacanthus `` seed '', turkish leopard, tormentilla, dronoki, troloaoi, nigerian berry `` fruit '', Chinese yam or yam (wild boar), nagiida (butcher bloom), naginata koju, nazuna, rapeseed , Peanuts or vertical blades (sword beans), Natsumikan, jujube (Otsuchi), Naniwaibara (gold cherry blossoms), navena (continuous), sea cucumbers, narco lily (yellow spirits), peanuts (peanuts), nanten (nantanami), nanban karamushi ( Sesame), Smoke violet, Nigaki (Nakiki), Nigamugi (Sweet), Nikuzuku, Kei or Nikkei or Ceylonni Kayak or yabuni kei (cinnamon) or cinnamon (katsushi), leek (lion), elderberry (bone, fruit, flower, stem, leaf), garlic (large persimmon), nurde (penta), leek, nemkin or neb or Nebi or Nemuroki or Jigokubana (community), Noazami (Otsuka), Noibara (fruit), Nozenkazura (Ryozenhana), Nogateto, Yarrow, Nodake (Maehu), Novara, Peach,

  Pineapple `` fruit '', hibiscus (Busouge, Fulin Bushouge, Roselle), Hailindou, Hauchiwa, Pau Doce, Hakamurabosi (bone crush), hakusen (white tine), Hakulberry `` fruit '', coconut (breeding), hazelnut (lion) ), Hasidorokoro (roth root), basil, lotus (lotus, lotus meat, lotus root), parsley (holland jellied), hadakamugi, batata, hachiku or madake (bamboo bamboo), patchouli, mint (thin load, thin load leaf), pearl barley ( (Yokuinin), Hanasuge (knowing mother), banana, Hanahaku, Hanabishi (Sitoshi, Shishitsuri), vanilla bins, papaya, scallops (Camellia), pahalobo, hub `` whole grass, stem, leaf '', paprika, clam or honey bee Vines), hamasge (kotsubushi), terrestris (spotted lion), hermanus (maikai flower), kingfisher (beach wind), hamamelis, palm, La (rose), balata, balata rubber tree, agaric (agaric, white-faced agaric, cuspidorium mosquito), halan, pauliurus (Amanicus japonicus), parietalia, balsamina (crane reishi, bitter gourd), haruni (crusted rind, cinnabar white skin, cocoon leaf), Barbasco, Harlin Dou, Handaikai (Aoi Okai), Pannoki, Hinogi (Shihi), Hikageturinjin (Party Affiliation), Pecan Nuts, Higanbana (Ishizuchi, Bujushahua), Hygurmadaria, Hishi (Rishimi), Pistachio, Beet , Hitsuba (Ishizuchi), Hitosuba Enishi, Daisies (Daisy), Hinata Taikozuchi (Cow Knee), Hinoki, Hiba, Castor, Sunflower, Peppers, Himezu (Tengu), Himegai (Kasumi), Himematsutake (Harimatsutake, Hiromatsutake) , Pimenta "fruit", beakshi, beakkyu, hiyu "fruit", belode oyster, hiro Okinagusa (bald eagle), loquat "fruits, leaves, stems", betel wax (large clothes, coconut), puer tea (Fuyu tea), Japanese quail (southern wicker), Japanese pufferfish, Japanese dandelion (Japanese winter flowers, Japanese winter leaf), Fujibacama (Orchid grass), Fuji bean (Tsuname), Fujimodoki (Chrysanthemum cherry, cocoon flower), Grape `` Fruits, pericarp, seeds, leaves '', Beech, Fuyushinatsusatake (Cycium edulis), Brazilian licorice, Brazilian carrot, Blackcurrant `` Fruits '' , Blackberry, Branjen, Plum `` Fruit '', Fruceraria, Blueberry, Prune, Floral Blanca, Blond Psyllium, Bundou (Mung Bean), Hazelnut, Loofah, Safflower (Safflower), Henken, Belladonna, Berry `` Fruit '', Percea, Pervianberg , Pereschia Grandifolia, Benquisium or Ikusa (Keiten), Boysenberry `` Fruit '', scallop or sardine or nendo or hachigi kokia (silk), spinach (daffodil, acute pupa, teleost), pouteria sapota, pouteria lucuma, cypress, burdock (windproof), spinach, physalis ( Toro), Hoonoki (Japanese Kokaku, Pak), Bokeh (Kiso), Hosoba Abraciku (Kayoyoku), Hosobao Guruma, Hosobana Okera (Samurai), Hosoba Kirinso (Keiten 37), Hosobabarengiku, Bodaiju (Ogi), Button (peony `` flower, leaf, stem, bark ''), button pi (peony skin), hop, jojoba, borage (borage), Bordeaux, white berry `` fruit '', Hononiku (meat juju, large art), Honsecok (Iron-Skin Stone Wall, Sparrow Mountain Stone Wall)

  Mizul Tennan Show (Tennan Star), Maitake (Maiko), Maou (Mao), Maca, Macadamia Nut, Margaret (Mokushungiku), Macri (Seaman Grass), Magwa (Mulberry White Bark `` Bark, Leaf ''), Magnolia Sprengeri, Masalan Suva, Masalan Suba de Ceara, Masheira de Boi, Mashua (Tamanousen Hallen, Kyukonkinrenka), Matatabi (Mushroom), Matsukasa, Pine "Leaf, Bark, Root", Matta Oryo, Matsuhodo (茯苓), Matico (Cordon Sillo), Mayoram (Hanahakka), Malvano Jazine (Bitter), Mulberry `` Fruit '', Quince, Malene (Bilaud Muzuka), Mango, Mangosteen, Manzanya (Andesca Mitsule), Manshuugurumi, Mandarin `` Fruits, Mannentake (Reishi), Pheasants (fruit berries), Mishima Saiko (Shibako), Japanese giant plantain or Japanese morning glory (Dragongrass), Mizokakushi (half-sided lotus), Misohagi (Chinese cypress), Japanese willow or Japanese willow (Henpo), Japanese oak, Mitsuba, Midorisapotate, Midorihakka, Mimosa, Miyuga, Miracle fruit `` Fruit '', Myrrh , Myrobalan, wheat straw, mugwort, mugwort, mugwort (prolonged skin), munya, murasaki (purple), murasakikibu or oomurasakikibu (purple pearl), murasaki corn, murasaki kinfuji (Kunming chicken blood wisteria), megakansa opuntica , Swordfish (beneficial herb), Meowugi, Melaroika, Melissa, Merirot, Melon `` Fruit '', Moukomugi, Mosouchiku, Mokukirin, Mogi (Pepper), Mokko (Mokuko), Momijidaio, Peach (Peach `` Leaf, Seeds, Flowers) , Fruit "), sprout, Morello cherry" fruit ", Moroheiya (burlap) , Yakawamura potato, Yakuchi (Michichi), cornflower (Yamagmagiku), cornflower mint, yacon, Yashabushi (Yagar) or Himeyashabushi or Ooyabayashabushi `` fruit, pericarp, fruit ear '', Yachiyanagi, starfish (octagonal gold plate), mistletoe (octagonal gold plate) (Willow parasitism), willow (river willow, prickly willow, weeping willow, red willow, cat willow, Incolyana, kinu willow, kolyanagi, unryu willow, Miyama willow, coconut, willow, sardine, gill , Yabukouji (purple gold beef), Yabu tobacco (crane candy, Tenmeisei), pokeweed (trade land), Yamahanoki (Yamabuchi), bayberry (Bamboo plum), sagebrush, eucalyptus, yukinoshita (tiger ear grass), yucca or flevifolia, Yuzu “fruits”, lilies, armor rushes, peaches Gi (bamboo leaves),

  Rigan (Raimaru), lime `` fruit '', rye, lacanca `` fruit '', raspberry `` leaves, fruit '', latania (chlamelia), rakkyo or shallot (lavender), lavender, longan (long-eyed meat), agave (aonogave agave, fuchlin) Agave), Ryokucha (green tea), apples `` fruits, seeds, leaves, roots '', gentian, rubus or suabisimus (cool), litchi (toothpick, toothpick core), lettuce (chisha), red currant `` fruit '', red pitaya , Lemon `` fruit '', lemongrass, forsythia or Chinese forsythia (ream), forsythia, wintersweet, wax palm, locon (reed, root), loganberry `` fruit '', rosemary (mannenrou), rosehip (nobara) ), Horseradish, cotton wisteria (Minghong flower), and bituminous (land).

  In addition, other seawaters such as sea salt, seawater dried products, dead sea or Atlantic or Pacific sea water (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud or mud (fango), such as Sea mud or mud from various locations such as Italy, Fango, German Fango, Eifel Fango, Freiburg Fango, etc. (Ingredients: silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, magnesium oxide, calcium oxide Strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic, water), saintoku stone, etc.

  As seaweeds, seaweed [Green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Aonori (Usba aonori, Sugiaonori, Hiraaoori, Boaonori, Hosoeda Aonori), Anaaoosa (Aosa)] (Macombu, Resilibu, Hosocome, Mitsuishi Kombu), Wakame, Hirome, Aowakame, Giant Kelp (Macrocystis Integrifolia, Neocitus Ruetkeana), Hijiki, Hibamata, Umiuchiwa, Usubaumiuchiwa Umiuchiwa, Akaba Umiuchiwa, Konami Uchiwa, Okinawa Uchiwa, Usuki Uchiwa, Etsumi Uchiwa], seaweed [Red algae: Hijirimen, Maca (Tengusa), Hiraksa, Syria, Oaxa Caterpillar, Yatabegusa, Yuukiri, Shimatengusa, Tosakanori, Togekirinsai, Amakusakirinsai, Giraffe, Juniper ginkgo rhinoceros, Tsunomata, Totsuka (Yahazutsu notamata), Ezotsunotamata, Togetu no Takita , Yaleus banori, Kagius banori, Susius banori, Haius banori, Akamo mizunori] and the like.

  In addition, other algae such as green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Kuwanomimo: Kuwanomimo, Volboxa: Ougemawari, Volbox, Palmera, Yotsumeo, Aomido: Chrysanthemum, Aoumidro, Tirguimidro, Hibidoro: Hibidoro, Aosa: Anaaaosa, Amiaosa, Nagaaosa, Kawanori: Kawanori, Friccella, Shiogusa Magama genus: Magamata, Iwagata: Fusaikawa, Surichogata, Heraiwata, Kurokita, Hanemo, Genus: Mill, Chromel, Sakibutimil, Nagamil, Hira Lemon, Casanori genus: Casanori, Jusmo genus: Jujusmo, Tamajusmo, Mizojuzumo, Mika ヅ kimo, Korekaete, Tsutsumimo genus: Kyukougusa, Hitogususa genus: Humanegusa, Hirohatohigususa, Umushihimoe, Sakikimoe Sumiremo: Sumiremo, Hoshimamidro, Fushinashimidoro etc.).

  Cyanobacteria (Suizenjinori: Suizinori, Aoko, Nenju: Kawatake, Ishijelly, Hassai, Yuremo, Rasenmo (Spirulina): Spirulina, Trichodesmium (Iaciasio), etc.

  Brown alga Sanadagusa: Sanadagusa, Fukurimamiji, Commongusa: Commongusa, Yahazugusa: Ezoyahaz, Yahazugusa, Uraboshiyahaz, Jigamusa: Jigmygusa, Umichiwagusa, Umamisakugakua, Hakusou : Navarimo, Chasomen genus: Motsuki Chasomen, Matsumo genus: Matsumo, Nagamatsumo genus: Nagamatsumo, Okinawa mozuku: Okinawa mozuku, fake Tomozuku: Niseft Mozuku, Futomozuku: Futomozuku, Ishimozuku: Ishimozuku, Kuromo: Kuromo, Nisemozuku: Nisemozuku, Mozuku: Mozuku, Ishige: Ishige, Iroro, Ichimegasa: Ichimegasa Umibosu, Urushigusu: Urushigusa, Keurushigusa, Tobaccogusa, Composita: Composita, Haberodomo: Haberodoki, Havanori: Habanori, Habanori: Habanori, Commonbukuro, Ezobukuro, Ezobukuro Chishima fukuronori genus: Chishima fukuronori, Kagomenori genus: Kagomenori, Muraridori genus: Murachidori, shark genus: shark zusa, genus genus: lobster, genus genus: yokojimamanori, Ganoderma genus: cynomolgus, fennel spider: fennel spider, vine genus: turmo, turtle genus: sea turtle, scorpion genus: sujime, misji kumbu genus: misji kumbu, atsuba misji kumbu, kumbu genus: tsutsugara kumbu, oyster kumbu, onikombu, gakonbubu Barracuda, Trollocombu: Trollocombu, Antokume: Antokume, Kajime: Kajime, Tsuruarame, Kurome, Kikuishikombu: Kikuishicombu, Nejikcombu: Nejicombu, Kuroshiome: Kuroshiome, Nekoshikonbu, Aramei Genus: Ainuwakame, Chigaiso, Oniwakame Ezoi Shige: Ezoi Shige, Yabane Moku: Yabane Moku, Rappa Moku: Rappa Moku, Joromoku: Ugano Moku, Jo Lomoku, Hiemoku, Honda Walla: Tamanashimoku, Isomoku, Nagashimamoku, Akamoku, Shidamoku, Honda Walla, Nejimoku, Narasamo, Mametawala, Tatsukuri, Yatsumata Moku, Umitora no Moku, Fushishimoku, Mohmokumoku, Hahamokumoku , Ukimo: Ouokimo, Burukimo: Burukimo, Kayamonori: Kayamonori, etc.).

  Red algae Genus: Sea urchins, Tsukumonori, Anemone crickets, Astragalus, Amaranthus, Amaranthus, Agaricata Genus: Tamaitadaki, Kaikekenori Genus: Kaginori, Kaikekenori, Tengusa: Himetengususa, Hytengususa, Oobusa, Nambugu , Kohira, Yohira, Kinukusa, Hibirodo: Hibirodo, Himebiro, Isomumedo: Isommedo, Aedes: Rhizome, Ryumons: Ryumon, Ranunculaceae, Nisekare Naminohana: Hosobanaminohana, Naminohana, Sangomodoki: Ragaramomodoki, Shiogusagoromo: Shiogusagoromo, Iwanokawa: Etsukiiwanawa, Kinokawa: Kinokawa, Kanonote, Sangori Kyono Shiro, Sakuranori, Nikumukade, Tambanori, Tsurutsuru, Fudarak, Marubahdarak, Isonohana: Nurakusa, Ronulaksa, Oomkadenori, Hirakintoki: Hirakintoki, Matabou: Matabou, Kintoki: Chabokintoki, Kintoki, Matsunori, Rice, Tokamatsu, Hitomatsu, Fushikintoki, Tsunomude, Nagakinito, Crayfish Nagaobane genus: Nagaobane, Funori genus: Hanafnori, Fukurofunori, Mafunori, Karekigusa: Karekigusa, Tosakamodoki: Hosobanosakamodoki, Hirohatosakamodoki,

  Yatsudegatatosakamodoki, Kurotosakamodoki, Nesanonotosakamodoki, Yusora, Etsukinotosakamodoki, Nankaitosakamodoki, Himetosakamodoki, Kinuhada: Kinuhada, Ezotosaka, Ezotosakari Hoonoo: Hounoo, Hikagenoit: Hikagenoit, Usuginu, Nikuhounoo: Nikuhounoo, Benisunago: Venice Nago, Susukabeni: Skakebeni, Okaragusa: Yamadagusa, Mirin: Mirin, Hosobamiri, Zochoe Genus: Isomocca, Yukari Genus: Yukari, Hosoyukari, Ibaranori: Ibaranori, Saidaibara, Tachiibara, Kagyibaranori, Kizino-o: Genus Kizinoo, Isodane Udonus: Isodantsu, Atsubanori: Atsubanori, Ogonori: Ogonori, Tsurushiramo, Shiramo, Oogo-nori, Mizo-okonori, Birch, Beniogonori, Fukureshinori, Purple Birch, Shinkabanori, Ryugo Species of the genus: Acer genus, Acer genus Genus: Lotus Jigusa, Sugiori Genus: Ikanoashi, Hosoibonori, Novonori, Black Hagininso: Black Haginanso, Akabaginanso Genus: Red-backed ginkgo, Hoshibukuro, Hashibukuro, Madaragusa: Togemadara, Etsukimadara, Taoyagisou: Taoyagiso, Hanasakura, Fukutsunagi: Fukutsunagi, Sugikonori, Hanadaeda, Hiratakadari , Uebusca genus: Uebusca, Benifukuronori genus: Benifuronori, Fusutunagi: Fusutunagi, Himefusunagi, Hirohafushitsunagi, Etsugienaga: Hirawatsugisou, Usubawatsunagisou, Igis genus: Igis, Keigis, Egret Phytophthora, Saeda: Saeda, Chile Momiji: Chile Momiji, Conanaceae: Habutaeori, Conanori, Suzuronori, Usbeni: Usbeni, Ha Sugiginu: Hasujiginu, Nagakonohanori Genus: Nagakonohanori, Sugiginu: Sugiginu, Atsubasjiginu, Heiusbanori: Kagiosubanori, Yareusubanori, Susiusbanori, Hiusubaniyaki, Usubanoirakiki, Enashidajia, Shimadadia genus: Isohagi, Shimadadia, Dajimodoki: Dajimodokuki, Otogusa: Moroitogusa, Futoigusa, Makuri genus: Makri, Willow genus: Hanayanagi, Yuna, Yanaginori, Motsurayazo, Sasazokori, Sasazozo Hanesozo, Sozonohana, Hanegusa: Hanegusa, Kehanegusa, Kozanemo: Kozanemo, Isomurasaki, Hosozozanemo, Himegoke: Himegoke, Rohmegokke, Hyodosig genus: Chrysanthemum terrestris, Hyodosigusa, Usubahiodoshi, Isomegususa, Isuzumegususa, Suginasugusa, Suibushigusa, Isobabushi genus: Isobasho, Fujimatsumomu, Fujimatsukiwa, Akisawa Ideyukogome: Ideyukogome, Okimozukoku: Okichimozu, Otogusa, Chinorimo: Chinorimo, Chisujinori: Chisujinori, etc.).

  Axial algae (Shajikumo, Shiratamamo, Hoshitsurimo: Hoshitsurimo, Rikunotumusu, Flaskmo: Himebumo, Chaboflasmo, Tripera, etc.), yellow algae (Hikarimo: Hikarimo, etc.), etc.

  In addition, the raw materials derived from animal sources include chicken crown extract, cow or human placenta extract, pig or cow stomach, duodenum or intestinal extract or degradation product thereof, pig or cow spleen extract or its Degradation products, extracts of porcine or bovine brain tissue, water-soluble collagen, collagen derivatives such as acylated collagen, collagen hydrolysates, elastin, elastin hydrolysates, water-soluble elastin derivatives, keratin and their degradation products or their Derivatives, silk proteins and degradation products thereof or derivatives thereof, porcine or bovine blood cell protein degradation products (globin peptides), porcine or bovine hemoglobin degradation products (hemin, hematin, heme, protoheme, heme iron, etc.), milk, casein and Decomposition product thereof or derivatives thereof, skim milk powder and decomposition product thereof or derivatives thereof, lactoferrin or decomposition product thereof, egg component Fish hydrolyzate, nucleic acid-related substance (ribonucleic acid, deoxyribonucleic acid), or the like.

(14) Microbial culture metabolites Yeast metabolites, yeast extract, natto metabolites, natto extract, rice fermented extract, rice bran (red potato, white birch) fermented extract, euglena extract, raw milk or skim milk lactic acid fermentation Products, trehalose or derivatives thereof.

(15) α-hydroxy acids glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(16) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, Bengala, black iron oxide, gunjo, chromium oxide, chromium hydroxide, carbon black, calamine, etc.

(17) UV absorber / blocker benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, sodium 2-hydroxy-4-methoxybenzophenone-5-sulfonate, dihydroxy Dimethoxybenzophenone, dihydroxydimethoxybenzophenone-sodium sulfonate, 2,4-dihydroxybenzophenone, tetrahydroxybenzophenone, etc.), p-aminobenzoic acid derivatives (paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, paradimethylaminobenzoic acid) Amyl, octyl paradimethylaminobenzoate, etc.), methoxycinnamate derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate, octyl paramethoxycinnamate, 2-ethoxycinnamic paracinnamic acid) , Sodium paramethoxycinnamate, potassium paramethoxycinnamate, diparamethoxycinnamate mono-2-ethylhexanoate glyceryl, etc.), salicylic acid derivatives (octyl salicylate, salicylphenyl, homomenthyl salicylate, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate, Methyl salicylate, etc.), anthranilic acid derivatives (methyl anthranilate, etc.), urocanic acid derivatives (urocanic acid, ethyl urocanate, etc.), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives , Camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives Umbelliferone, esculin, benzyl cinnamate, cinoxate, oxybenzone, dioxybenzone, Okutabenzon, sulisobenzone, benzo resorcinol, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, Neo Heliopan, ESCA rolls, zinc oxide, talc, kaolin.

(18) Whitening agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound, benzotriazole derivative, tetrazole derivative, imidazoline derivative, pyrimidine derivative, dioxane derivative, camphor derivative, furan derivative, pyrone Derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin C or derivatives thereof (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), vitamin E or derivatives thereof, kojic acid or derivatives thereof, oxybenzone, benzophenone, arbutin, Guaiazulene, shikonin, baicalin, baicalein, berberine, placental extract, ellagic acid, lucinol, etc.

(19) Tyrosinase activity inhibitor Vitamin C or a derivative thereof (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), hydroquinone or a derivative thereof (hydroquinone benzyl ether, etc.), kojic acid or a derivative thereof, vitamin E or a derivative thereof, N-acetyltyrosine or its derivatives, glutathione, hydrogen peroxide, zinc peroxide, placenta extract, ellagic acid, arbutin, lucinol, silk extract, plant extract (camille, mulberry, scented, firewood, clara, mugwort, honeysuckle, yellowfin) , Dokudami, matsuhod, pearl barley, nettle, hops, hawthorn, eucalyptus, yarrow, altea, keihi, mankaishi, hamamelis, yamagwa, lifelong grass, bellflower, toshishi, goblin, shooting dried, mao, senkyu, dokatsu, rhinoceros , Windproof, littoralis, Scutellaria, peony bark, peony, cranesbill, kudzu root, licorice, Fushi aloe, Cimicifuga, safflower, green tea, black tea, Ali elixir), and the like.

(20) Melanin pigment reduction / decomposition substance Phenylmercury hexachlorophene, mercuric oxide, mercuric chloride, hydrogen peroxide, zinc peroxide, hydroquinone or its derivative (hydroquinone benzyl ether).

(21) Turnover promoting action / cell activator hydroquinone, lactic acid bacteria extract, placenta extract, ganoderma extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (aloe, Ogon, Japanese horsetail, Gentian, Burdock, Shikon, Carrot, Camelis, Hop, Yokuinin, Odori, Semburi, Japanese cypress, Japanese red snapper, Achacha, Hypericum, Cucumber, Necklace, Rosemary, Parsley).

(22) Astringents Succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid (including catechin compounds) and the like.

(23) Active oxygen scavenger
SOD, catalase, glutathione peroxidase and the like.

(24) Antioxidants Vitamin C or salts thereof, stearic acid ester, vitamin E or derivatives thereof, nordihydrogua ceretenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole , Propyl gallate, sesamol, sesamorin, gossypol, propolis, etc.

(25) Lipid peroxide production inhibitor β-carotene, plant extract (sesame culture cell, hamcha, hypericum, hamamelis, clove, melissa, enamel, birch, salvia, rosemary, minamitenji, agetsu, ginkgo, green tea).

(26) Anti-inflammatory agent ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor, dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or its Salt, glycyrrhetinic acid or a salt thereof, licorice extract, sicon extract, age extract, propolis, etc.

(27) Antibacterial / bactericidal / disinfectant Acrinol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or hydrolyzate thereof, alkyldiaminoethylglycine chloride solution, triclosan, sodium hypochlorite, chloramine T, Sarashi powder, iodine compound, iodoform, sorbic acid or its salt, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p-chlorophenol, p- Chloro-m-xylenol, p-chloro-m-cresol, thymol, phenethyl alcohol, o-phenylphenol, Irgasan CH3565, halocarban, hexachlorophene, chlorohexidine, ethanol, methanol, Sopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, phenoxyethanol, chlorobutanol, isopropylmethylphenol, nonionic surfactant (polyoxyethylene lauryl ether, polyoxyethylene nonyl phenyl ether, polyoxyethylene octyl phenyl ether, etc.), Amphoteric surfactants, anionic surfactants (sodium lauryl sulfate, potassium lauroyl sarcosine, etc.), cationic surfactants (cetyltrimethylammonium bromide, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride), formaldehyde, hexamine, brilliant green, Malachite Green, Crystal Violet, Jamal, Photosensitive Element 101, Photosensitive Element 201, Photosensitive Element 40 No., N- long chain acyl basic amino acid derivative and its acid shark salts, zinc oxide, hinokitiol, Sophora root, propolis, and the like.

(28) Moisturizer Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin or chitosan derivative, pyrrolidone carboxylic acid or its salt, sodium lactate, mini Sasanishiki extract, natto bacteria metabolite, natto extract, loofah water, birch (white birch) or red pine sap,

(29) Elastase activity inhibitor Diisopropyl fluorophosphate, plant extract (eg, gongoid, hypericum, clara, mulberry leaves, keihi, genokosho, comfrey, salvia, elderberry, bodaiju, button pi), seaweed extract and the like.

(30) Hair preparation.
Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castari tincture, pepper tincture, red pepper tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, sodium bromate, thioglycolic acid and the like.

(31) Antiandrogens Follicular hormones (estrone, estradiol, ethinyl estradiol, etc.), isoflavones, oxendrone, etc.

(32) Peripheral vascular blood flow promoter Vitamin E or derivatives thereof, assembly extract, garlic extract, carrot extract, aloe extract, gentian extract, toki extract, cephalanthin, carpronium chloride, minoxidil and the like.

(33) Stimulants Pepper tincture, nonyl acid vanillamide, cantalis tincture, ginger tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(34) Metabolic activator Photosensitive element 301, hinokitiol, pantothenic acid or derivatives thereof, allantoin, placental extract, biotin, pentadecanoic acid glyceride and the like.

(35) Antiseborrheic agent Pyridoxine or a derivative thereof, sulfur, vitamin B6 and the like.

(36) Keratolytic agent Resorcin, salicylic acid, lactic acid and the like.

(37) Oxidizing agent Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, sodium pyrophosphate, Sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract, etc.

(38) Depilatory agents Inorganic reducing agents such as strontium sulfate, sodium sulfide, barium sulfide, calcium sulfide, thioglycolic acid or its salts (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate) Strontium thioglycolate) and the like.

(39) Hair swelling agents such as ethanolamine, urea and guanidine.

(40) Dye
5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'-iminodiphenol, 2,4-diaminophenoxyethanol hydrochloride 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, orthoaminophenol, N-phenyl acetate Paraphenylenediamine, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-diamine, nitroparaphenylenediamine, paraaminophenol, para Nitroorthophenylenediamine, paraphenylenediamine, paramethylamino Phenol, picramic acid, sodium picramate, N, N'-bis (4-aminophenyl) -2,5-diamino-1,4-quinonediimine, 5- (2-hydroxyethylamino) -2-methylphenol, N -Phenylparaphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate, orthoaminophenol sulfate, orthochloroparaphenylenediamine sulfate, 4,4'-diamino sulfate Diphenylamine, 2,4-diaminophenol sulfate, toluene-2,5-diamine sulfate, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthophenylenediamine sulfate, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, parasulfate sulfate Methylaminophenol, metaaminosulfate Diol, metaphenylenediamine sulfate, catechol, diphenylamine, α-naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcin, tannic acid, 2-hydroxy-5-nitro-2 ', 4'-diaminoazobenzene-5'-sulfone Sodium acid, hematein, etc.

(41) Fragrance Natural animal fragrance such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guayakwood essential oil, Cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western peppermint essential oil, Otsuka perfume essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsam essential oil, buttery essential oil, rose essential oil, palmarosa essential oil, persimmon essential oil, hiba essential oil, sandalwood essential oil, petit gren essential oil, bay essential oil, vetiva essential oil, bergamot Spirit , Peru balsam essential oil, boad rose essential oil, melamine essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, Japanese mint essential oil, and other synthetic flavors Fragrance etc.

(42) Dye and Colorant Red Cabbage Dye, Red Rice Dye, Akane Dye, Anato Dye, Ikumi Dye, Turmeric Dye, Enju Dye, Krill Dye, Amber Dye, Caramel, Gold, Silver, Gardenia Dye, Corn Dye, Onion Dye , Tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin etc.

  Others, moisturizers, hormones, sequestering agents, pH adjusters, chelating agents, antiseptic and antibacterial agents, cooling agents, stabilizers, emulsifiers, animal and vegetable proteins and their degradation products, animals and plants Saccharides and their degradation products, animal and plant glycoproteins and their degradation products, blood flow promoters, anti-inflammatory agents, antiallergic agents, cell activators, keratolytic agents, wound treatment agents, foam enhancers, thickeners, oral cavity Additives, deodorizing / deodorizing agents, bittering agents, seasonings, enzymes and the like can be mentioned, and by using them in combination, various additive and synergistic effects can be expected.

  The dosage form of the polylysine preparation or cosmetic composition of the present invention is arbitrary, and can be used in the form of capsules, powders, granules, solids, liquids, gels, bubbles, emulsions, creams, ointments, sheets and the like.

  Specifically, for example, external pharmaceutical preparations, lotions, emulsions, creams, ointments, lotions, oils, packs, and other basic cosmetics, facial cleansers and skin cleansers, hair removers, hair removers, after shave lotions, preshaves Makeup cosmetics such as lotion, shaving cream, foundation, lipstick, blusher, eye shadow, eyeliner, mascara, perfume, nail polish, nail enamel, nail enamel remover, shampoo, rinse, hair treatment, pre Hair treatments, hair conditioners, permanents, hair nicks, hair dyes, hair cosmetics such as hair growth and hair nourishing agents, bath preparations, deodorants, deodorants, poultices, plasters, tapes, sheets, patches, Suitable for various products such as aerosols, toothpastes, mouth fresheners and gargles, sanitary cotton, wet tissue, etc. It can, also, can also be used to common foods and drinks.

  In addition, about the addition method to the polylysine formulation or cosmetic composition of this invention, it may add previously, may be added in the middle of manufacture, and should just select it suitably considering workability | operativity.

  EXAMPLES Hereinafter, although it demonstrates still in detail according to an Example, this invention is not limited to these Examples. The ε-polylysine used in this example was a 0.25 wt% aqueous solution obtained by diluting a 25 wt% ε-polylysine aqueous solution manufactured by Chisso Corporation 100 times with water.

(Test 1) Histamine release inhibition test In the immediate allergic reaction, chemical substances such as histamine are released from mast cells or basophils sensitized in the second stage. Therefore, a substance that suppresses histamine release can be expected to have an antiallergic action. In this test, a test method (J. Soc. Cosmet. Japan, Vol. 25, No. 4, P246 (1992)) that releases compound histamine from rat mast cells with compound 48/80, a histamine release reagent, was used.

“Test method and evaluation method” In a 1.2 mL mast cell suspension collected from the abdominal cavity of rats (Slc: Wister male rats, about 4-9 weeks old), 0.02 mL of the sample and compound 48/80 were added at a final concentration of 1.0 μg / It added to mL, and it incubated at 37 degreeC for 15 minute (s). After stopping the reaction by cooling with ice, the reaction solution is centrifuged, and histamine released from the supernatant is extracted and purified. Colored with o-phthaldialdehyde, and the fluorescence absorbance at excitation wavelength of 360 nm and fluorescence wavelength of 450 nm is measured. The histamine release inhibition rate was determined from Equation 1, and the results are shown in FIG. As a comparative sample, cromolyn (manufactured by Sigma) was prepared under the same conditions and used for the test.

<Equation 1> Histamine release inhibition rate (%) = {1- (A−C) / (B−C)} × 100
A: fluorescence intensity of histamine released when a sample was coexisted with mast cells and a histamine releasing agent was added,
B: fluorescence intensity of histamine released when a histamine releasing agent was added to mast cells,
C: Fluorescence intensity of histamine naturally released from mast cells (A, B, C are the measured values minus the blind values).

(Test Results) As shown in FIG. 1, it was confirmed that the ε-polylysine of the present invention has an action of significantly suppressing histamine release. In addition, compared with cromolyn, it can be confirmed that it has a very strong histamine release inhibitory action and can be used as an antiallergic agent.

(Test 2) Safety test (1) Skin primary irritation test ε-polylysine prepared in purified water so that the dry solid content concentration is 0.5% and shaved back of Japanese white rabbit (female, 1 It was applied to the skin of 3 animals in a group weighing approximately 2.3 kg. 24, 48, and 72 hours after application, evaluation was performed using erythema and edema as an index based on the criteria for Draise. As a result, in all animals, no erythema and edema were observed, and it was judged that there was no problem with primary skin irritation.

(Test 3) Safety test (2) Cumulative skin irritation test Hartley guinea pigs (female, 1 group) prepared with purified water with ε-polylysine having a dry solids concentration of 0.5% and shaved back 0.5 mL / animal was applied to the skin of 3 animals (approx. 320 g body weight) once a day, 5 times a week. Application was performed over 2 weeks and shaving was performed on the last application day of each week. Evaluation was performed using erythema and edema as indices based on the criteria for Draise on the day after each application and the day after the last application. As a result, in all animals, no erythema and edema were observed for 2 weeks, and it was judged that there was no problem with respect to cumulative skin irritation.

(Test 4) Safety test (3) Acute toxicity test ddy mice (male and female, 1 group, 5 animals, 5 weeks old) fasted for 4 hours before testing ε-polylysine powder obtained by vacuum concentration and drying ) Was orally administered at a dose of 5,000 mg / kg, and the onset and degree of toxic symptoms were observed over time. As a result, no abnormalities were observed in all mice for 14 days, and no abnormalities were found in the anatomical results. Therefore, LD50 is 5,000 mg / kg or more, and is judged as a safe substance.

(Prescription example) Manufacture of antiallergic agent, histamine release inhibitor or cosmetic composition According to the above evaluation results, the formulation examples are shown below. Only the blending amount was shown. The present invention is not limited to these. In addition, in the following formulation examples, for those whose pH falls outside the range of 4.5 or more and less than 6.5, the pH is adjusted to 4.5 or more and less than 6.5 with a citric acid-sodium citrate buffer did.

(Formulation Example 1) Emulsion
weight%
1. Squalane 8.0
2. Jojoba oil 7.0
3.Cetyl alcohol 1.5
4.Glycerin monostearate 2.0
5.Polyoxyethylene (20) cetyl ether 3.0
6. Polyoxyethylene (20) sorbitan monooleate 2.0
7.1,3-Butylene glycol 1.0
8.Glycerin 2.0
9.ε-Polylysine 1.0
10. Preservative (paraoxybenzoic acid ester) appropriate amount
11.Perfume appropriate amount
12. Citric acid-sodium citrate buffer
13. Residue with 100% total purified water

(Prescription Example 2) Emulsion
weight%
1. Squalane 8.0
2. Jojoba oil 7.0
3.Glyceryl paraaminobenzoate 1.0
4.ethyl urocanate 1.0
5. Vitamin C phosphate magnesium salt 1.0
6.Cetyl alcohol 1.5
7. Glycerin monostearate 2.0
8.Polyoxyethylene (20) cetyl ether 3.0
9.Polyoxyethylene (20) soorbitan monooleate 2.0
10.1,3-Butylene glycol 1.0
11.Glycerin 2.0
12.ε-Polylysine 0.6
13. Preservative (paraoxybenzoic acid ester) appropriate amount
14. Fragrance (lavender water) appropriate amount
15 Citric acid-sodium citrate buffer
16. Residue with 100% total purified water

(Formulation example 3) Cosmetic oil
weight%
1.liquid paraffin 30.0
2.Squalane 20.0
3.Olive oil 20.0
4.Isopropyl palmitate 10.0
5.ε-Polylysine 0.5
6. Umiuchiwa hot water extract 2.0
7. Elcampre hot water extract 2.0
8. Grapefruit fruit or leaf hot water extract 1.0
9. Shea fat 1.0
10.Butylhydroxyanisole 0.1
11. Vitamin E derivative 0.1
12. Preservative (benzalkonium chloride) appropriate amount
13. Perfume (apple water) appropriate amount
14. Citric acid-sodium citrate buffer
15. Residue with 100% total purified water

(Formulation Example 4) Flexible lotion
weight%
1.Glycerin 5.0
2.1,3-Butylene glycol 5.0
3.Polyoxyethylene sorbitan monolaurate (20E.O) 1.0
4.Ethanol 15.0
5.ε-Polylysine 0.3
6. Toki hot water extract 2.0
7. Hijirimen 50% ethanol extract 1.0
8. Horse chestnut whole plant 50% ethanol extract 1.0
9.Leaf or stem 50% ethanol extract 1.0
10. Appropriate amount of antibacterial / preservative (lactoferrin solution)
11. Fragrance (Western light water) appropriate amount
12. Residue with 100% total purified water

(Formulation Example 5) Astringent lotion
weight%
1.Sorbit 2.0
2. POE (20) oleyl alcohol 1.0
3.Ethanol 15.0
4.ε-polylysine 0.5
5. Hibamata 50% glycerin extract 2.0
6. Green tea leaf or stem 50% glycerin extract 2.0
7. Gentian whole plant 50% glycerin extract 2.0
8. Zeny mushroom whole plant 50% glycerin extract 2.0
9.Allantoin 0.2
10. Astringent (Zinc Sulfocolate) 0.2
11. Antibacterial and antiseptic (thiamin lauryl sulfate) appropriate amount
12. Perfume (Chen skin water) appropriate amount
13. Citric acid-sodium citrate buffer
14. Residue with 100% total purified water

(Formulation Example 6) Two-layer lotion (liquid to liquid type)
weight%
1. Squalane 8.0
2. POE sorbitan tetraoleate 0.3
3.Sorbit 1.0
4.Ethanol 15.0
5.ε-Polylysine 0.3
6. Mulberry white skin 70% ethanol extract powder 1.0
7. Savonso 50% ethanol extract 1.0
8. Senkyu rhizome 30% ethanol extract 2.0
9. Sage whole plant 30% ethanol extract 2.0
10. Fuquidan popo flower and leaf 30% ethanol extract 1.0
11. Pomelo fruit 30% ethanol extract 1.0
12.Ascorbic acid 0.2
13. Astringent (calamine) 0.1
14. Preservative (phenoxyethanol) appropriate amount
15. Perfume (mandarin orange) appropriate amount
16. Residue with 100% total purified water

(Formulation Example 7) Carmine lotion (liquid to solid)
weight%
1.Glycerin 3.0
2.1,3-Butylene glycol 5.0
3.Ethanol 15.0
4. Iron oxide (Bengara) 0.2
5.Zinc oxide 0.5
6.Kaolin 2.0
7.ε-Polylysine 0.5
8. Glaze 50% ethanol extract 1.0
9. Ishizuchi fruit 50% propylene glycol extract 1.0
10. Hypericum flower or leaf 50% propylene glycol extract 1.0
11. Placenta extract 1.0
12.Camphor 0.2
13.Phenol 0.03
14. Preservative (photosensitive element 201 & 401) appropriate amount
15. Perfume (Ouren water) appropriate amount
16. Residue with 100% total purified water

(Prescription Example 8) Pack
weight%
1.Polyvinyl alcohol 15.0
2.Carboxymethylcellulose sodium 5.0
3.Propylene glycol 3.0
4.Ethanol 10.0
5.ε-Polylysine 0.3
6. Camelis leaf or bark 70% 1,3-butylene glycol extract 3.0
7. Sakuraba 50% ethanol extract 5.0
8. Peach leaf 50% ethanol extract 5.0
9. Hosobabarengiku (Echinashi) whole plant 30% ethanol extract 2.0
10. Eucalyptus leaf or stem 30% ethanol extract 2.0
11. Preservative (paraoxybenzoic acid ester) appropriate amount
12. Perfume (Bodaiju water) appropriate amount
13. Residue with 100% total purified water

(Formulation example 9) Peel-off pack
weight%
1.Glycerin 6.0
2.Propylene glycol 4.0
3.Polyvinyl alcohol 15.0
4.Ethanol 10.0
5.Polyoxyethylene glycol 1.0
6. Pantothenyl ethyl ether 1.0
7.ε-Polylysine 0.2
8. Scented rhizome 50% ethanol extract 1.0
9. Capsicum 30% ethanol extract 1.0
10. Hosobabareniku (Echinashi) leaf 30% ethanol extract 1.0
11. Arnica flower or leaf 30% ethanol extract 1.0
12. Preservative (paraoxybenzoic acid ester) appropriate amount
13. Perfume (Kashiwa distilled water) appropriate amount
14. Residue with 100% total purified water

(Formulation Example 10) Cold cream
weight%
1. Sarah Beeswax 11.0
2.liquid paraffin 22.0
3.Lanoline 10.0
4.Olive oil 5.0
5. Chamomile oil 5.0
6. Palm oil 5.0
7.borax 0.5
8.ε-Polylysine 0.2
9.Oat bark extract 2.0
(Ethanol: 1,3-butylene glycol = 1: 1 extraction solvent)
10. Birch bark extract 2.0
(Ethanol: 1,3-butylene glycol = 1: 1 extraction solvent)
11. Comfrey leaf extract 2.0
(Ethanol: 1,3-butylene glycol = 1: 1 extraction solvent)
12. Parietalia 50% ethanol extract 2.0
(Ethanol: 1,3-butylene glycol = 1: 1 extraction solvent)
13.Liquid shea fat 1.0
14. Preservative (Acrinol) 0.1
15. Perfume (sage water) appropriate amount
16. Residue with 100% total purified water

(Prescription Example 11) Emollient cream
weight%
1.Stearic acid 2.0
2. Stearyl alcohol 7.0
3.Reduced lanolin 2.0
4.Squalane 5.0
5. Octyldodecanol 6.0
6. Polyoxyethylene cetyl ether (25E.O.) 3.0
7. Lipophilic glyceryl monostearate 2.0
8.Propylene glycol 5.0
9.ε-Polylysine 0.1
10.Suzuki 50% 1,3-butylene glycol extract 3.0
11. Wild time 50% 1,3-butylene glycol extract 3.0
12. Chimp skin 50% 1,3-butylene glycol extract 3.0
13. Pine fruit 50% 1,3-butylene glycol extract 3.0
14. Orange peel 50% 1,3-butylene glycol extract 3.0
15. Preservative (phenoxyethanol) appropriate amount
16. Perfume (chome water) appropriate amount
17. Residue with 100% total purified water

(Prescription Example 12) Face Wash Cream
weight%
1.Myristic acid 25.0
2.Stearic acid 5.0
3. Tallow fatty acid 5.0
4.Propylene glycol 10.0
5.Potassium hydroxide 6.0
6. Palm oil fatty acid diethanolamide 6.0
7.ε-Polylysine 0.2
8. Raspberry fruit 50% 1,3-butylene glycol extract 2.0
9. Persimmon leaves 50% 1,3-butylene glycol extract 2.0
10. Western elderflower or berries
50% 1,3-butylene glycol extract 2.0
11. Chamomile flower 50% 1,3-butylene glycol extract 2.0
12. Soybean 50% 1,3-butylene glycol extract 2.0
13.Dipotassium glycyrrhetinate 1.0
14.Apricot kernel 0.1
15. Preservative (salicylic acid) 0.1
16. Fragrance (sage water) appropriate amount
17. Residue with 100% total purified water

(Prescription Example 13) Face Wash Cream
weight%
1.Stearic acid 10.0
2.palmitic acid 10.0
3.Myristic acid 12.0
4.Lauric acid 4.0
5.Oleyl alcohol 1.5
6. Lanolin derivative (EO adduct) 1.0
7.Glycerin 18.0
8.Potassium hydroxide 6.0
9.ε-Polylysine 0.2
10. Royal Jelly Extract 2.0
(Ethanol: 1,3-butylene glycol = 1: 1 extract)
11.Glycyrrhizic acid 1.0
12. Antiseptic (salicylic acid) 0.1
13. Walnut kernel 0.1
14.Corn cob grain 0.1
15. Fragrance (rose water) appropriate amount
16. Residue with 100% total purified water

(Prescription Example 14) Body soap
weight%
1.Potassium laurate 15.0
2.Potassium myristate 5.0
3.Propylene glycol 5.0
4. Whey 1.5
5.ε-Polylysine 0.3
6. Birch sap 10.0
7.Carrageenan 5.0
8. Persimmon leaf 70% 1,3-butylene glycol extract 0.5
9. Kiwi fruit 70% 1,3-butylene glycol extract 0.5
10. Ginseng root 70% 1,3-butylene glycol extract 0.5
11. Natto metabolite 0.5
12.Polyethylene powder 0.5
13.Hydroxypropyl chitosan solution 0.5
14. Amino acids (Glycine, Glutamine, etc.) 0.5
15. Preservative (Undecylenic acid, phenol)
16.pH adjusting agent
17. Perfume (shower water) appropriate amount
18. Residue with 100% total purified water

(Prescription example 15) Sunscreen cosmetics (O / W type)
weight%
1.Oxybenzone 2.0
2.Octyl paramethoxycinnamate 5.0
3.Squalane 10.0
4.Vaseline 5.0
5. Stearyl alcohol 3.0
6.Stearic acid 3.0
7. Glyceryl monostearate 2.0
8.Polyethyl acrylate 1.0
9.1,3-Butylene glycol 6.0
10.Edetate disodium 0.1
11.Triethanolamine 1.0
12.Titanium dioxide 5.0
13.ε-Polylysine 0.1
14. Horsetail 70% dipropylene glycol extract 0.5
15. Peony skin 50% dipropylene glycol extract 0.5
16. Birch bark 50% dipropylene glycol extract 0.5
17. Hypericum whole plant 50% dipropylene glycol extract 0.5
18. Saiko Root 50% Dipropylene Glycol Extract 0.5
19. Preservative (paraben) appropriate amount
20. Perfume (apple water) appropriate amount
21. Residue with 100% total purified water

(Formulation Example 16) Sunscreen cosmetics (oil type)
weight%
1.liquid paraffin 70.0
2.Ethyl paraaminobenzoate 1.0
3.Tetrahydroxybenzophenone 1.0
4.Cetyl octanoate 25.0
5.ε-Polylysine 0.3
6. Sancha Flower Whole Water Extract 0.5
7. Glaze root or leaf water extract 0.5
8. Hop whole plant water extract 0.5
9.Ginger rhizome water extract 0.5
10.Tachi musk whole plant water extract 0.5
11.Comfrey water extract 0.5
12.Dihydroxymethoxybenzophenone 0.2
13. Antioxidant (Butylhydroxytoluene)
14. Fragrance (jasmine water) appropriate amount

(Prescription Example 17) Cream foundation (O / W type)
weight%
1.Stearic acid 4.0
2.Glycerol monostearate3.0
3.Cetanol 1.5
4.Isopropyl myristate 7.0
5. Liquid paraffin 10.0
6. Sarah Beeswax 3.0
7.Triethanolamine 1.5
8.Glycerin 3.0
9. Bentonite 1.0
10.Titanium oxide 8.0
11.Titanium mica 3.0
12.2,4-Dihydroxybenzophenone 8.0
13.Kaolin 3.0
14. Talc 3.0
15.ε-Polylysine 0.2
16. Centella leaf or stem 70% ethylene glycol extracted dry powder 0.2
17. Mulberry leaf 70% ethylene glycol extracted dry powder 0.2
18. Coloring pigment (yellow iron oxide) 1.0
19. Coloring pigment (Bengara) 1.0
20. Preservative (paraben) appropriate amount
21. Fragrance (white sand water) appropriate amount
22. Residue with 100% total purified water

(Prescription Example 18) Shampoo
weight%
1.Lauryl sulfate triethanolamine 5.0
2. Sodium polyoxyethylene lauryl ether sulfate 12.0
3.1,3-Butylene glycol 4.0
4.Lauric acid diethanolamide 2.0
5. Edetate disodium 0.1
6.ε-polylysine 0.5
7. Usvenia mallow leaves 30% 1,2-butylene glycol extract 2.0
8. Mulberry white skin 30% 1,2-butylene glycol extract 2.0
9.Kakkon 30% 1,2-butylene glycol extract 1.0
10.Vegetable squalane 1.0
11.Hydroxypropyl chitosan solution 2.0
12. Mucopolysaccharide solution 1.0
13. Antibacterial and antiseptic (photosensitive element 401)
14.Adjustable pH adjuster
15. Fragrance (Hamamelis water) appropriate amount
16. Residue with 100% total purified water

(Prescription Example 19) Rinse
weight%
1. Stearyltrimethylammonium chloride 2.0
2.Cetostearyl alcohol 2.0
3.Polyoxyethylene lanolin ether 3.0
4.Propylene glycol 5.0
5.ε-Polylysine 0.2
6. Chamomile flower or stem 50% 2,3-butylene glycol extract 2.0
7. 50% 2,3-butylene glycol extract from bonito leaves or stems 2.0
8. Forsythia leaf 50% 2,3-butylene glycol extract 0.1
9.Hydroxyethyl chitosan solution 1.0
10. Natto metabolite 0.5
11. Trehalose solution 0.1
12. Hydrolyzed silk solution 1.0
13. Antibacterial and antiseptic (benzalkonium chloride) appropriate amount
14. Fragrance (mugwort water) appropriate amount
15. Residue with 100% total purified water

(Prescription Example 20) Rinse
weight%
1. Stearyltrimethylammonium chloride 2.0
2.Cetostearyl alcohol 2.0
3.Polyoxyethylene lanolin ether 3.0
4.Propylene glycol 5.0
5.Ethylene glycol 3.0
6.ε-Polylysine 0.3
7. Forsythia fruit 50% ethylene glycol extract 2.0
8. pearl seed 50% ethylene glycol extract 2.0
9. Trehalose solution 2.0
10. Hydrolyzed keratin solution 2.0
11.Silicon resin solution 2.0
12. Antibacterial and antiseptic (benzalkonium chloride) appropriate amount
13. Perfume (camomile water) appropriate amount
14. Residue with 100% total purified water

(Prescription Example 21) Hair Liquid
weight%
1.Ethanol 29.0
2.Polyoxypropylene butyl ether phosphate 10.0
3.Polyoxypropylene monobutyl ether 5.0
4.Triethanolamine 1.0
5.ε-Polylysine 0.2
6. Glaze hot water extract 0.5
7.Avocado hot water extract 0.5
8.Spicy whole hot water extract 1.0
9. Ginkgo Leaf Hot Water Extract 1.0
10.Carpronium chloride 0.3
11. Hinokitiol 0.5
12.Keratin degradation solution 1.0
13. Antibacterial and antiseptic (paraben, benzalkonium chloride) appropriate amount
14. Fragrance (rose water) appropriate amount
15. Residue with 100% total purified water

(Formulation Example 22) Hair Liquid
weight%
1.Ethanol 29.0
2.Polyoxypropylene butyl ether phosphate 10.0
3.Polyoxypropylene monobutyl ether 5.0
4.Triethanolamine 1.0
5.Trimethylene glycol 5.0
6.ε-polylysine 0.1
7. Wild edible whole plant 50% ethanol extract 1.0
8. Artemisia whole plant 50% ethanol extract 1.0
9. Oolong tea leaves or seeds 50% ethanol extract 1.0
10.azulene 1.0
11. Antibacterial and antiseptic (paraben, benzalkonium chloride) appropriate amount
12. Fragrance (Western light water) appropriate amount
13. Residue with 100% total purified water

(Prescription Example 23) Hair Nick
weight%
1.Ethanol 40.0
2.Ethyl oleate 1.0
3.Polyoxyethylene (40) hydrogenated castor oil 2.0
4.ε-Polylysine 0.3
5. Peony skin 60% ethanol extract 3.0
6. Coconut leaf or fruit 60% ethanol extract 3.0
7. Preservative (paraben) appropriate amount
8.Residue with 100% total purified water

(Prescription Example 24) Hair Nick
weight%
1. l-Menthol 0.2
2.Ethanol 32.5
3.Polyoxyethylene hydrogenated castor oil 0.5
4.Propylene glycol 5.0
5.Diphenhydramine hydrochloride 0.3
6.ε-Polylysine 0.2
7. Rakan fruit hot water extract 1.0
8.Ouren rhizome hot water extract 1.0
9. Whole corn hot water extract 1.0
10. Chicken crown extract solution 1.0
11.Carboxymethylchitin solution 1.0
12.Silicon resin 0.5
13. Vitamin E derivative 0.5
14. Antibacterial and antiseptic (paraben) 0.1
15. Fragrance (cornflower water) appropriate amount
16. Citric acid-sodium citrate buffer
17. Residue with 100% total purified water

(Formulation Example 25) Hair growth / hair growth agent
weight%
1.Ethanol 60.0
2.ε-Polylysine 0.3
3. Ginseng whole plant 50% propylene glycol extract 5.0
4. Green tea (leaves or seeds) 50% propylene glycol extract 2.0
5. Yarrow fruit 50% propylene glycol extract 1.0
6. Vitamin E derivative 0.5
7.Pepper tincture 0.5
8.Resorcin 0.5
9.Glycyrrhizic acid 0.5
10.Hydroxypropyl chitosan solution 0.5
11.Bovine hematin solution 0.5
12.Soy protein hydrolysis solution 0.5
13.Mucin solution 0.5
14. Amino acids (Glycine, Glutamine, etc.) 0.5
15. Antibacterial and antiseptic (paraben) 0.1
16. Fragrance (kiwi water) appropriate amount
17. Residue with 100% total purified water

(Prescription Example 26) Hair growth / hair growth agent
weight%
1.Ethanol 60.0
2.ε-Polylysine 0.2
3. Tree bark or fruit 50% 1,3-butylene glycol extract 1.0
4. Tomorrow's 50% 1,3-butylene glycol extract 1.0
5. Aspergillus nuclei 50% 1,3-butylene glycol extract 1.0
6. Lotah whole plant 50% 1,3-butylene glycol extract 0.5
7. Cantalis tincture 0.5
8.Pantothenic acid derivative 0.5
9.Resorcin 0.5
10.Glycyrrhizic acid 0.5
11.salicylic acid 0.5
12 Hydroxyethyl chitosan solution 0.5
13.Elastin hydrolyzate 0.5
14. Antibacterial and antiseptic (paraben) 0.1
15. Perfume (lavender water) appropriate amount
16. Residue with 100% total purified water

(Prescription Example 27) Granule bath preparation
weight%
1.Sodium bicarbonate 50.0
2.Anhydrous sodium sulfate 30.0
3.borax 5.0
4.ε-polylysine 0.5
5. Assembly hot water extraction dry powder 3.0
6. Achillea millet hot water extraction dry powder 3.0
7. Western thin leaf or stem hot water extract dry powder 3.0
8. Yukinoshita whole plant hot water extraction dry powder 3.0
9. Pigment (Gunjo green) appropriate amount

(Prescription Example 28) Granule bath preparation
weight%
1.Sodium bicarbonate 58.0
2.Anhydrous sodium sulfate 30.0
3. Borax 4.0
4.ε-Polylysine 0.2
5. Cornflower flower or leaf stem 70% ethanol extracted dry powder 2.0
6. Summer mandarin fruit or peel 70% ethanol extracted dry powder 2.0
7. Aloe pulp 70% ethanol extracted dry powder 2.0
8. Dye (Gunjou, chromium hydroxide) appropriate amount
9. Fragrance (orange water) appropriate amount

(Prescription Example 29) A coating solution containing an appropriate amount of ε-polylysine, antibiotics, anti-inflammatory agent and the like is impregnated into a coating protective agent gauze or liniment cloth, and attached to the wound. Further, ε-polylysine may be directly sprayed locally and coated with gauze or the like.

(Test 5) Use effect test The effect at the time of actually using the skin-care cosmetic composition of this invention was examined. The test is glossy, unskinned skin and / or people suffering from eczema, acne, rough skin, 10 to 30 years old each as a panelist, daily, twice daily in the morning and night, after washing the face, the appropriate amount of the emulsion of Formulation Example 1 Was applied to the face for 3 months. In addition, the thing which prescribed | regulated what remove | excluded (epsilon) -polylysine from the emulsion by the same method was used for the control | contrast. Moreover, the evaluation method is performed according to the following criteria, and the results are as shown in Tables 1 and 2. The numerical values in the tables represent the number of people. No one complained of skin abnormalities during the period of use.

"Skin feel improvement effect"
Effective: Increased skin gloss and tension.
Slightly effective: Skin gloss / tension increased slightly.
Invalid: No change before use.

"Eczema / rough skin improvement effect"
Effective: Eczema, acne and rough skin were improved.
Slightly effective: Eczema, acne, rough skin were slightly improved.
Invalid: No change before use.

(Test results) As shown in Tables 1 and 2, the cosmetic composition containing ε-polylysine of the present invention has been confirmed to have increased skin gloss and tension and to improve eczema, acne and rough skin. It was.

(Test 6) Use effect test The effect at the time of actually using the cosmetic composition of this invention was examined. The use test is conducted by applying 10 persons aged 30 to 60 years old who are suffering from dry skin as a panelist, applying the appropriate amount of the emulsion of Formulation Example 2 to the face for 3 months after washing their face every morning and night. went. In addition, the thing which prescribed | regulated what remove | excluded (epsilon) -polylysine from the emulsion by the same method was used for the control | contrast. The evaluation method is based on the following criteria, and the results are as shown in Table 3. The numerical values in the table represent the number of people. No one complained of skin abnormalities during the period of use.

"Dry skin improvement effect"
Effective: Improved dryness of the skin.
Slightly effective: Dryness of skin was slightly improved.
Invalid: No change before use.

(Test results) As shown in Table 3, it was confirmed that the cosmetic composition containing ε-polylysine of the present invention significantly improved skin drying.

The figure which showed the histamine release inhibitory effect of (epsilon) -polylysine of this invention.

Claims (2)

  An antiallergic agent comprising ε-polylysine.   A histamine release inhibitor comprising ε-polylysine.

Images