JP2001002550A - Olive leaf extract, application to skin preparation for external use or bathing agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a melamine production inhibitor or an active oxygen- eliminating agent by including an olive leaf extract as an active ingredient, and to obtain a skin preparation for external use or a bathing agent which has excellent usability and an effective melamine production-inhibiting action, improves a dry skin, eczema and skin roughness, and gives luster and firmness to the skin and hair, by including the melamine production inhibitor or the active oxygen-eliminating agent. SOLUTION: This melamine production inhibitor of active oxygen-eliminating agent contains an olive leaf extract as an active ingredient preferably in an amount of about 0.01 to 20 wt.% as the solid content. The extract is obtained, for example, by extracting olive leaves with an extraction solvent such as purified water usually at ordinary temperature to the boiling point of the solvent under the atmospheric pressure and then purifying the extract by a filtration method and/or by a method using an ion exchange resin. The agent is useful for milky lotions, soft skin lotions, astringent skin lotions, and so on.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a novel cosmetic effect of olive leaf extract and its application to a skin external preparation or bath preparation based on the same.

[0002] The external preparation for skin or bath preparation according to the present invention refers to all external preparations for preventing or improving pigmentation such as spots and freckles, rough skin, and rough skin. Specifically, ampules, capsules, pills, tablets, powders, granules, solids, liquids,
1) Topical pharmaceuticals, 2) quasi-drugs, 3) Topical or systemic skin cosmetics (eg, basic makeup such as lotion, emulsion, cream, ointment, lotion, oil, pack, etc.) Ingredients, facial cleansers and skin cleansers, massage agents,
Cleansing agent, hair remover, hair remover, shaving treatment fee, aftershave lotion, pre-show lotion,
Shaving creams, etc.), 4) Medicinal or cosmetic preparations applied to the scalp / hair (eg, shampoos, rinses, treatments, permanent solutions, hair dyes, hair styling agents, hair tonics, hair restoration / hair restoration, etc.) ), 5) Bath agent to be poured into bath water, 6) Others, axillary deodorant, deodorant, deodorant, sanitary goods, sanitary cotton, wet tissue, mouth freshener (eg, toothpaste, mouthwash, etc.) And the like.

[0003]

2. Description of the Related Art Factors determining human skin color include the amount of melanin in the epidermis, blood flow in capillaries, pigments derived from food (carotene), and the thickness of the stratum corneum. The most important factor is melanin pigment. The so-called liver spots (stains), sparrow egg spots (buckwheat), and pigmentation of the skin after sunburn are markedly enhanced in the production of melanin pigments due to the activation of pigment cells present in the skin. This is one of the major concerns for women.

[0004] The melanin pigment is produced by melanosomes, which are organelles in pigment cells (melanocytes) present in the basal layer of the epidermis, the hair root, and the outer root sheath. In the process of melanin production, tyrosinase is activated by tyrosine in pigment cells (melanocytes) to oxidize tyrosine,
Dopa, converted to dopaquinone, further autoxidized, polymerized via dopachrome, 5,6-dihydroxyindole,
Eventually it becomes melanin pigment. The melanin thus produced is secreted by the dendrites of pigment cells (melanocytes) into basal cells, and the basal cells divide, become spiny cells, rise and reach the stratum corneum, and then fall off as the stratum corneum detaches. I will do it.

[0005] In order to prevent or improve skin spots, spots and freckles, and dark spots on the skin, it is conceivable to inhibit the production process of melanin or to lighten the already produced melanin. Can be Based on these, various whitening components have been conventionally proposed. For example, arbutin, kojic acid or a derivative thereof, ascorbic acid or a derivative thereof,
Tocopherol or a derivative thereof, glutathione, hydrogen peroxide, zinc peroxide, hydroquinone monobenzyl ether, placenta extract, silk extract and plant extract (arnica, altea, aloe, ogon, emmeiso, chamomile, licorice, gardenia, genoshoko, sicon) , Ginger, birch, senkyu, gennoshoko, psycho, sasanqua, toki, calendula, elderberry, safflower, garlic, adlay, litchi, logwood)
Etc. are known.

On the other hand, in recent years, the production of active oxygen in a living body and various effects (diseases) caused thereby have been reported. Generally, this active oxygen is also called Activated oxygens, and includes O2-, H2O2, OH, and chemiluminescence.
They are broadly classified into species, all of which have a strong bactericidal action and are considered important substances involved in the self-defense of living organisms. For example,
When foreign enemies (antigens) such as bacteria, viruses, and foreign substances enter the living body, phagocytic neutrophils, monocytes, and macrophages in the blood first initiate phagocytosis, and then the phagocytic endoplasmic reticulum Active oxygen is produced to dissolve foreign substances engulfed therein. This active oxygen acts on the dissolution of phagocytic foods, and on the other hand, directly exerts a bactericidal action on external enemies such as bacteria and foreign substances, thereby playing a role of protecting the living body from external enemies.

However, if the active oxygen for self-defense is excessively produced and secreted, even normal cells may be lysed and stimulated, resulting in various disorders.
Recently, many diseases / diseases induced by active oxygen have been reported. For example, fats and oils such as cholesterol in blood are oxidized by active oxygen, and vascular disorders due to arteriosclerosis in the brain and heart (stroke, myocardial infarction, etc.)
And blood flow impairment due to a thrombus or the like at the time of cerebral trauma, and ischemic state (oxygen deficiency) in blood vessels due to sudden transient atrophy.

[0008] Regarding the relationship between the skin and active oxygen, for example, the skin is an organ that is in direct contact with the outside world, and thus is susceptible to environmental factors. If the active oxygen continues to be excessive in the skin due to ultraviolet rays or radiation, it may react with unsaturated fatty acids of the phospholipids of the biological membrane to generate lipid peroxide. It is feared that the generated lipid peroxide induces arteriosclerosis, carcinogenesis, aging, membrane destruction, protein degeneration, skin inflammation, edema, wrinkles and the like. In addition, as a disease due to excess active oxygen, from a cosmetic point of view, for example, herpes dermatitis, X-ray dermatitis, burns, trauma, sunshine dermatitis, contact dermatitis, eczema, atopic dermatitis, etc. are concerned. You.

[0009] In order to prevent or ameliorate a disease such as the one described above, for example, superoxide dismutase (SOD), catalase, glutathione peroxidase, In addition, tocopherols (vitamin E), oryzanol, plant extracts (hamamelis, melissa, emmeiso, birch, sage, rosemary, age, basil, ginkgo, psycho, peonies, hange, cahig, tissou, ougon, carrot, licorice, ginger) Its usefulness has been pointed out.

[0010]

However, the above-mentioned whitening components or active oxygen-eliminating substances have poor stability and solubility in a prescription system, and give off odors and coloring due to sedimentation and decomposition, as well as biological substances. It was not satisfactory in practical use, for example, the effect at the level was not sufficient, and there were safety problems such as skin irritation.

In view of these circumstances, the present inventors have conducted intensive research on a natural material which is excellent in usability and has an effective melanin production inhibitory action and an active oxygen scavenging (SOD-like) action. Olive leaf extract is found to be a useful ingredient that satisfies the title task,
By utilizing this, an external preparation for skin or a bath for improving dry skin, eczema, rough skin, giving luster and firmness to skin and hair, and the like was completed.

[0012]

SUMMARY OF THE INVENTION The present invention relates to a melanin production inhibitor containing olive leaf extract as an active ingredient, an active oxygen scavenger, or an external preparation for skin or bath containing olive leaf extract.

[0013]

BEST MODE FOR CARRYING OUT THE INVENTION "Olive" used in the present invention is a plant of the family Oleaceae or Olea.
Olives (Olea europaea Linne) use their leaves, but may include stems, branches, and branches. Other congeners, for example, O. welwitschii, O. paniculata, and the like can also be used.

A plant that has been squeezed out as it is or after crushing. Alternatively, it is extracted with a solvent as it is or after pulverization.
Examples of the extraction solvent include water, alcohols (for example, lower alcohols such as methanol, anhydrous ethanol and ethanol, or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), ketones such as acetone, ethyl ether, and dioxane. , Acetonitrile, ethyl acetate and the like can be used alone or in combination of two or more.

The olive leaf extract can be arbitrarily prepared by drying, concentrating, diluting, or mixing with other components, depending on the dosage form and form to be applied.

The extraction is usually carried out under normal pressure at a temperature ranging from room temperature to the boiling point of the solvent. After the extraction, the solution is purified by filtration and / or an ion exchange resin to obtain a solution, paste, gel, or powder. I do. In many cases, the extract can be used as it is, but if necessary, purification treatment such as deodorization and decolorization can be performed as long as the effects of the present invention are not affected. In addition, as a purification treatment means such as deodorization and decolorization, an activated carbon column or the like can be used.

The olive leaf extract of the present invention can be used as a melanin production inhibitor, an active oxygen scavenger, or as a raw material to be incorporated into a skin external preparation or bath preparation. The content thereof varies somewhat depending on the type of the applied dosage form, the method of use, and the expected degree of action, but is usually 0.001% by weight or more (hereinafter, expressed as% by weight) as solid content, and preferably about 0.01 to 20%. .

The melanin production inhibitor, active oxygen scavenger, external preparation for skin or bath agent according to the present invention may further comprise, in addition to the above-mentioned essential extract, a range which does not impair the effects of the present invention.
It can be produced by arbitrarily selecting and using the components and additives exemplified below.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow fatty acid, kukui nut oil, safflower oil, shea butter, soybean oil, camellia oil, corn oil, rapeseed oil,
Persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, mocro, coconut oil, tallow, horse oil, lard or hydrogenated products of these oils (hardened oil) Etc., etc.

(2) waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, rice wax, squalene, squalane, pristane, etc.

(3) Mineral oil Liquid paraffin, vaseline, paraffin, ozokeride, ceresin, microcrystalline wax and the like.

(4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid , 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2
-Synthetic fatty acids such as ethylhexanoic acid and isopentanoic acid.

(5) alcohols ethanol, isopiranol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, and phytosterol, and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) Gum, sugar or water-soluble polymer compound Arabic gum, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose or its ester, trehalose or its derivative, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, alkylene such as ethylene oxide (C2-C4)
Oxide-added hydroxyalkyl (C2-C
4) chitin or chitosan, low molecular weight chitin or chitosan,
Chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or a salt thereof, hyaluronic acid or a salt thereof, chondroitin sulfate or a salt thereof, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose , Hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyalkylene oxide such as polyethylene oxide or polypropylene oxide or a crosslinked polymer thereof, carboxyvinyl polymer, Polyethyleneimine and the like.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt) ), Amphoteric surfactant:
Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic) Surfactants, ether ester nonionic surfactants, ester nonionic surfactants, block polymer nonionic surfactants, nitrogen-containing nonionic surfactants, and other surfactants (natural surfactants) , Derivatives of protein hydrolysates,
Polymer surfactants, surfactants containing titanium and silicon,
Fluorocarbon surfactants).

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A
1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B)
6), cyanocobalamin (vitamin B12), folic acids,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: vitamin C acid or a derivative thereof, vitamin D group: ergocalciferol (vitamin D2), cholecalciferol (vitamin D3),
Dihydrotaxerol, vitamin E group: tocopherol, tocotrienol or derivatives thereof, ubiquinones, vitamin K group: phytonadione (vitamin K1),
Menaquinone (vitamin K2), menadione (vitamin K
3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (rutin, eriocitrin, hesperidin), vitamin U and the like.

(12) Various amino acids valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine, and the like, and their sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These are processed in a usual manner according to the type and form of the product to be added (for example, pulverization, milling, washing, hydrolysis, Fermentation, purification, squeezing, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc. Select to serve.

The solvent used in the case of extraction is selected in consideration of the purpose and type of the product to be provided, and the processing to be performed later. Usually, water and a water-soluble organic solvent (eg, ethanol) are used. , Propylene glycol, 1,
Or a mixture of two or more selected from 3-butylene glycol). However, when the content of the organic solvent is not preferable depending on the application, use only water, or employ ethanol which is easily removed after extraction, or use alone or in an arbitrary mixture with water,
Or simply use what is extracted and extracted.

When an additive derived from a plant or animal material is used for a systemic or topical external preparation or cosmetics, the skin and hair are protected, the moisturizing, the feel and texture are improved, and the flexibility is imparted. Relieve irritation, relieve stress by aroma, activate cells (prevent cell aging), suppress inflammation, prevent spots and freckles, improve skin and hair quality, prevent rough skin and improve it,
Cosmetic effects such as hair growth, hair growth, hair loss prevention, gloss imparting, cleansing effect, alleviation of fatigue, blood flow promotion, warm bath effect, and other functions such as fragrance, deodorant, thickening, antiseptic, buffering An additive or synergistic effect can be expected.

Specific plants (crude drugs) as raw materials include:
For example, almonds (Hentomo), eye (Aiba), Blue Kazura
(Seifu Fuji), Aoki (Aoki), Aokiri or Kenasia Ogiri
(Otogi), Aotsumuraji (Kibo), Acacia, Akashoma (Akashoma), Akasuguri "fruit", Akagrape, Akaminoki (Logwood), Akamegawiwa (Akabu Kashiwa), Akane
(Akane grass root), Acacia elegans or Jiou (Jihuang), Agi (Awei), Akinire (Royu skin), Aguahe (Omitengu palm), Akebi (Kidori), Asa (Ajihito), Malva Asagao or Asagao (Towushiko) , Hydrangea (hydrangea), ashitaba (morning leaves), azuki (red azuki bean), acerola, asenyaku (asenyaku), acira (shokuyoukanna), anise, abemaki `` fruit '', avocado, ama, amacha (sweet tea), amachaper, Amadorokoro (Jade bamboo), Amana (Kojimitsu), Amaranthus (Hyuyu, Hagaito, Himogeito, Senninkoku,
Japanese cedar
(Haribiyu, Amaranthus hippochondriax), Amigasa lily or Bimo (shellfish), Algarrobo (cabbage),
Althea, Arnica, Alpinia or Katsudaidai (Azuma-ku), Aloe (lux), Aloe vera, Angelica, Apricot or Hon-azu (Almond jelly), Ansoko (Azusa), Ikakozolina (Biliary head), Epimedium or Periwinkle (Inyokaku), Igusa (Wicked grass), Knotweed (Tiger root), Yew (No. 1), Strawberries, Figs (Apricot `` Fruit,
Leaf)), Ichihatsu (first time), Ichibi (Fruit Aoi), Ichiyakusou (one medicinal herb), Ginkgo (Ginkgo `` seed, leaf ''), Itohimemegi (Enshi), Carob, Inu-zuna (Telelexi), Inubeyu (Sorainubiyu), Rice `` seed, seed coat '', Inondo `` seed '', Ibuki-kusou-so, nettle, ylang-ylang, iwa-tobacco (iwatsuchi-chi), iwahiba or iwamatsu (makikashiwa), oolong tea, wikyo (fenka), wheat (tibusanoki), ukiyagara (miyoko) ), Uguisukagura "fruit",
Himeuikyo, Turmeric (Utsukin), Duckweed (Floating), Usubasaishin or Keirinsaishin or Shousaisaishin (Spicy), Usvenia oi, Usvenitai oi, Utsubogusa (summer dry grass), Udo or Shishiudo (Qang, Independent, Tang Independent) ), Unyadegato (Unkaria, Cat's claw), Uba tea, Ume (Ume `` seed, pulp ''), Vladimir, Owaurushi (Kusaco peach), Unshu mandarin (Chen skin), Estragon, Ezocogi (Ezo Goka), Echinashi (Hosobamurasakisakirengiku) , Enishida, Enokitake (Enotake), Ebisugusa or Cassia Tora (Kyoko), Elcampre, Elderberry `` fruit '', Elemi, Elingy or Prelotus elinge, Endu (Solanaceae, Sophoraceae rice), Ogi or Kibanaugi (yellow) Aspergillus (Ogi), Ouren (Yellow), Ookarasuri (Karokon), Ooguruma (Civil engineering) ), Giant Tsudzurafuji (spelled), psyllium (psyllium, psyllium), Oohashibami
(Haruko), Obanana okera or Okera (white jujutsu), Obananasasuberi (Banaba), Obanayasabushi "fruit", Omihasashi or Hawthorn (Yamako), Ousei (Nalco lily, Kagikurumabanarukoyuri), Ohisasanoyuki or Sasanogi ogi, Oosakasayukigi, Osamugi ogi , Giant grasshopper (cereal grass),
Oka, Okazeri (snake bed), Oguruma (turnover), Okra `` fruit '', Panax ginseng or carrot (carrot), Hypericum or Kogomebai perforated or Periwinkle (Brother's cut grass), Odorikosou (intermittent), Onamimomi (blue ears), Onigurumi, Onidokoro or Tokoro or Nagadokoro (Hikai), Oninoyagara (Tianma), Oniyuri or Sasasayuri or Hakatayuri (Yuri), Ononis, Halibut (Hibaha Yu), Ominaeshi (Loose), Oyuko, Dutch mustard (Crasson), Dutch jelly , Dutch honeybee, olives "fruits, seeds, leaves", oregano, orange "fruits, pericarp", calyxio (ripe yellow), cacao "fruits, pericarp,
Seeds '', persimmons (persimmons, `` leaves, fruits ''), stalks (hooks), persimmons or licorice (lotus), cassia,
Kasinaki (Kozomi "fruits"), Gajutsu (Shazajutsu), Kashiwa (Kazuki, Kazaha), Cascarilla, Cascarasagrada, Kanixa
(Kin Sato), Kaniwa, Kanokoso (Yoshikone), Birch or weeping birch (white birch), Pumpkin, Capoknoki `` seed '', Kahokoku Sansho (shopper), Gama (Kabao), Chamomile or Roman chamomile, Camilletula (tsukusa), Kamkamu (duckling), crow crow or cinnamon cucumber (gold melon), crow squirrel (midsummer), oak wheat, guarana `` seed '', karahoo (thick), karaya, karin (gull), garcinia, kawamidori, kawara saiko Hakubaku), Kawajisha,
Kawatake mushroom, Kawaranadeshiko (stone bamboo) or Ezokawaranadeshiko (Qumugi, Quumiko), Carrot ginseng (Aotoko), Kawara mugwort (Inchinko), Kansui (Asutsu), Kanzo (Licorice), Kantarasa, Candelilla, Kantou, Canna, Rubus
(Ezo strawberry, Dutch strawberry, Shrimp strawberry, Sawberry strawberry, Maple strawberry, European strawberry), Kiwi `` fruit, leaf '', Kikarasuuri (Ururone), Kikyo
(Kikyo, Kikyo root), Chrysanthemum (Chrysanthemum, Shima kaniku, Datura), Catalpa (Azumitsu), Cedar (Yotei root), Pheasant (Kikumi), Kizuta, Kidachi aloe, Kidachi mint, Kina, Kinoki (Shincona, Red linden), Quinua (Quinoa), yellowfin (yellow), gymnema sylvesta, cabbage, cabbage `` immature fruit '', cucumber, gyoryu (Nishikawayanagi, teriyaki), Kiraja saponaria, Kiraya, Kiranso (gold pea grass), kumquat `` fruit '', Betel, Kinkihiki (Senakuraku), Guava "fruit", Guayule, Quercus Infectria (Gallery), Kukuinuts, Kuko
(Gugi, gugi, gugi leaf, ground skin), Kusagi Kazura (Tenmen Fuyu), Kuzu (Kakkon), camphor tree, gooseberry `` fruit '', Xining ginseng (Yellow flower), Gardenia (Sanjiko), Kunugi (Bokusou) , Cupuas, kumazasa, black pine (horse whip grass), clara (bitter grass), cranberry `` fruit '', chestnut `` seed, fruit, astringent skin '', curculigo latifolia `` fruit '', grapefruit `` fruit and leaf '', buckthorn, black squirrel Necessary), Kurobana Hiokikoshi or Hiokiokoshi (extended life grass), Clove (clove, clove), Gumbinazu (Sekimei, Sekimeishi), Kaigai (Ikutake, Ikutakeho), Kaito (Rooster corolla, Chicken pupil), Gakekei (laurel tree), Kenashi Sartorii rose (Tokubukuryo, Yamakiko), gentian, Gennoshoko (Oirou grass), Kemponashi (Kigushi),
Koukisekoku, Kishumitsukan (Koji, Tachibana,
Olivenimikan, Fukuromikan, Sagamikouji, Ponkan, Santara (Tachibana skin)), Koushinbara (Monthly flower), Kosuui mint, Kozo `` fruit '', Kocha (tea), Kohone
(River bone), kouhon (straw and kara straw), kouriang, kourikyo (takara kang), cilantro `` fruit '', koren
(Hou Lian), Scutellaria (Yellow Gon), Cowberry (Koshibachi), Coco Palm `` Fruit '', Goshuyu (Goshuyu), Gosho Strawberry (Ubonoko), Pepper (Pepper), Copaiba Balsam, Coffee `` Seeds, Leaves '', Kobushi or Magnolia (hot spring), burdock (burdock, burdock), kobotdul, sesame (sesame), sesame seeds (ginseng), beetle (gomiko), Sanekazura or Binankazura or Matsubusa, wheat (wheat), rice or rice bran `` red bran, white bran ”, Rice oil, cola acuminata“ seed ”, cola
Vera `` seed '', fenugreek `` fruit '', colombo, kondulango, kelp, konjac, comfrey (finnari grass), sisal (sisal asa), saxima button pulp or sina button pulp or cinnamon pear (weird sac), sakura (oshima sakura, yamazakura, Oyamazakura, Edhigashi, Mamezakura, Miyamazakura, Someiyoshino, Takanezakura, Kasumizakura, Choujizakura, Kohigan, Satozakura, Kanzakura `` leaves, flowers, fruits, bark (cherry bark) ''), cherries, pomegranate, sasa, sasanqua, sajimodaka , Sweet potato, sugar cane, sugar beet, sapling jujube (acid jujube), saffron (red safflower, western safflower), pomelo `` fruit '', saponso, salmon berry `` fruit '', sarasin pear (masuma), salvia (sage), sagikyo ( Yamabana), Sawagurumi (Yamato) Thang Rede Grado
(Croton), Sansuiyousou, Sansin ginseng (37 ginseng), Sanshuyu (sanshuyu), Sansho (sansho), Sandscon (Yamazone), shea (carite), cyanoki `` fruit '',
Shiitake mushroom (Shiitake), Shion (Shion), Dichtaris, Shikunshi
(Kimiko), Perilla or Aodiso or Chirimenzo or Catamendiso (Shiyo leaf, Shiso), Rosewood, Shinanoki, Shinanohonoki, Sinarenkyo (ream fortune), Shimeji (Hinshimeji,
Shaka shimeji, hatake shimeji, ossiloi shimeji, bunashimeji, honjimeshi, shirotamogitake),
Potato, Peony (Shakuyaku), Shajin (Sasan), Jasmine (Matsurika), Janohige (Motomon winter), Sukushamitsu (Sandin, Shredded sand), Juzudama, Palm "fruit", Ginger (Ginger), Giant hydrangea (Tsuneyama), Shobu (iris, iris root), Shozuku `` fruit '', Shirakashi `` seed '',
White-headed butterfly `` seed '', white adzuki bean (chicken bone), white bean iris (small iris), white banana (flower), white nananten (minamitenmi), cinchona sassil, brassicae (mizuka, mizuka, shencho), cinnamon, watermelon (watermelon) ), Honeysuckle (gold and silver flowers, Shinobi winter), sorrel (acid pattern), swimberry `` fruit '', stevia, strawberry `` fruit '',
Suzu Psycho (Xu Chang Lord), Horsetail (Thorn), Purslane (Horse Tongue, Horse Tongue), Plum "Fruit", Pinus sylvestris "Pine cone", Larch, L., Kuzuta, S. aurumum, L. hawthorn, L. dandelion, L. Horse chestnut (malonie), pear `` fruit '', snowy pear, elderberry, elderberry, northern pear (juniper, juniper), yarrow
(Milfoil), rose, cornflower, mistletoe, mint or mint, horseradish, horseradish, sekisho (stone iris), sekikoku (stone mushroom), sedron (boshboku), mallow,
Hiroha Senega, Senega, Seri, Celery, Senkyu (River Kyu), Senshinren (Shinshinren), Sendan, Assembly
(Drug), senna `` fruits and leaves '', senninso (great tate), souka (grass), buckwheat `` seed seeds '', soybean potato, rhubarb
(Ohta), radish, soybean, daidai (orange, kikomi), takasaburo (dry lotus), takatodai (daigei), takawarabi (gull), dark sweet cherry `` fruit '', hollyhock,
Tachijakoso (thyme, Hyoka), Tachidokoro (Hikari), Tachiyanagi, Tamarind `` seed '', onion, tamashiba (spicy), taro (fruit, leaf, root bark), talwi, tanjin (dansan), dandelion (gakkohide) or shirobana Dandelion or moko dandelion, dammar, cherry `` fruit '', chiyaya `` fruit, root, bud '', chikusetsu carrot (bamboo ginseng), chicory, chancapiedra (Kidachikomikansou), kosensendaiou (daihuang), kosensen (buoy) , Ginseng (Ginseng), Artemisia (Ginseng), Choreimaitake (Irei), Tsukimiso, Tsukuritake (Mushroom), Camellia, Camellia, Spikeweed (Lacquer soup grass), Tsuyukusa (Duckweed grass), Tsuruazuki (Red azuki bean) , Tsurukudami (Hanshu crow), Tsuruna (Ban apricot), Cran carrot (Four leaf ginseng), Tsubaki, Deiko, Teuchigurumi, Du Lee: "fruit", Gelidium, boiling tea (Tien-cha), Tendaiuyaku
(Crowd drug), Pepper (Furry gourd), Pepper sparrow, Pepper
(Pan pepper), Touki (Toki), Tokinsenka (Marigold), Tonabena (River cut), Maize or Maize hair (Nanbaru hair), Tomozumomochi (Female Sadako), Toudodo or Korean gooseberry (dragon bile), Tokusa (Wood pirates), dokudami (jujutsu), radish (root spit), red sea bream or mamedaoshi or scutellaria, eucommia (mushroom `` bark, leaves, roots ''), ash (skin), tomato, tragacanth, triacanthos `` seed '', tolmentilla, Dronoki, Trolloa mallow, Niseria berry `` fruit '', potato or yam (yakuyaku),
Naginika (butcher bloom), Naginata Kouju,
Nazuna, oilseed rape, oil bean or sword bean (sword bean), jujube, jujube (daijutsu), nanawaibara (gold cherry blossom), navena
(Intermittent), nameko, narco lily (yellow), peanut (peanut), nanten (minamitenmi), nambangarashi (ramie),
Nigaki (bitter tree), bitter mugwort (bitter), nutmeg, kei or nikkei or ceylon nikkei or yabnikkei (cinnamon bark)
Or Keishi (Keishi), Chinese chive (Nirako), elderberry (bone-bearing tree `` fruit, flower, stem, leaf ''), garlic (garlic), nulde (fivefold child), leek, nemoki or neb or nebi or nemlinoki or zigokubana ( Healing), Nozami (Ohi), Noibara (Fruit), Yarrow, Nodake (Maehu), Nobara, Nomomo,
Palm, pineapple `` fruit '', hibiscus (Bussouge, Furinbussouge, Roselle), Hakamauraboshi (bone crushing), Hakusen (ringworm skin), Huckleberry `` Fruits '', Hakobe (Horuko), Hashibami (Haruko), Hashidokoro
(Roth root), basil, lotus (lotus, lotus, lotus), parsley
(Netherland jelly), naked mussel, batata, bee or medake (bamboo boiled), patchouli, mint (lightly loaded, lightly loaded leaves), barley (yokuinin), hanasuge (chimbo), banana, hana mint, hanabishi (situri, shisuturi), vanilla bins , Papaya, japonicus (rat koji), pahalobobo, hub "whole plant, stem, leaf", paprika, hamago or honeysuckle
(Thorn vines), Hamasuge (Kazuki), Hamabishi (Situriko), Hamanas (Maikai flower), Hamabuu (Hama windbreak), Hamamelis, Rose (Roses), Haratake (Haratake, Shilo-Oharatake, Usukimori no Kasa), Haran, Parietaria , Balsamina (Tsurureishi, bitter gourd), harunire (yu bark, yu white bark, yuba), hand dai kai (makidai sea), breadfruit, giant forage (sun dried), lizard carrot (party party), puer tea (pu-cha), pecan nut , Amaryllidaceae (Shirubi, Tsurumi Hana), Hishi (Hisami), Pistachio, Beet, Hitotsuba (Stone reed), Aitutsubasa (Echinacea purpurea), Hinata no Kozuchi (cow knee), Hinoki, Hiba, Castor, Sunflower, Bell pepper, Himeuzu
(Tamaoi), Himegama (incense blossom), Himematsutake (Kawariharatake, Hiromatsutake), Pimenta `` fruit '', juniper, beakkyuu, hiyu `` fruit '', belowudo oi, hirohaokinagusa (baldow), loquat `` fruit, leaf, stem '', Betel nut
(Large clothes skin, betel nut), Spodoptera serrata (southern rattan), Butterflies, Butterbur dandelion (Winter flower, Winter leaf), Fujibama (Orchid grass), Fuji bean (Tanto), Grape `` fruit, peel, seed, leaf '', beech, fu Yumsinatsukutake (Cordyceps), Brazilian Licorice, Brazilian Carrot, Black Currant `` Fruit '', Blackberry, Plum `` Fruit '', Flucellaria, Blueberry (Prunus avium), Prune, Blonde Psyllium, Bundou (Mung Bean), Loofah, Safflower (Safflower), Henneken, Belladonna, Berry `` fruit '', Peruvian Berg,
Benikeaiso or duckweed (Keiten), boysenberry `` fruit '', broomflower or elderweed or nendo or hawakigi kokia (skin), spinach (horizon, acute offspring, osteophyte), boufu (windbreak), spinach, physalis ( Toron), bonito (Japanese thick and magnolia), bokeh (gourd tree), bonsai (green aurora), bonsai tree (Satoshi Jitsuten), bonsai valley, bodaiju (bodai tree), button (peony, peony bark), hop , Jojoba, Bordeaux, Whittle berry "fruit", Hong Onik (Nikuju Ryo, Daigei), Hong Sekkok
(Iron bark stone, sparrow mountain stone mascara), myzurutennansha (tennanboshi), maitake (maitake), ephedra (mao), maca, macadamia nut, macri (sea grass), magua (mulberry white bark `` bark,
Leaf ''), magnolia or splengeri, mashua (Tamanouzenhallen, Kyuukonkinrenka), matabi (Mokuten tate), Matsukasa, pine `` leaves, bark, roots '', matsuhodo (bukuryo), matico (cordoncillo), mayolam ( (Hanamint), Malvano Jadine (Difficult), Mulberry `` fruit '',
Quince, mango, mangosteen, mansagna (andesukamitsure), manshu walnut, mandarin `` fruit '', mannentake (reishi), pheasant (kikomi `` fruit ''), mishimasaiko (saiko), mizuobako or mizuasagao (dragon tongue grass), Mizokokushi (half-edge lotus), misohagi (senkakuna), beet willow or elder willow (edition), mitsugashiwa, honeywort, midorihakka, mimosa, myoga, miracle fruit `` fruit '', myrrh, mirobaran, mukuge (mokugeki), mukunoki,
Mukuroji (surviving skin), Munya, purple (purple root), purple or purple purple, purple purple maize, purple purple mackerel (Kunming chicken blood wisteria), red seaweed (mother grass), mebogi, melaleuca, melissa, melilot, melon `` Fruit '', sagebrush, moso bamboo, moje (pepper blossom), mokko (wood incense), maple blossom, peach (peach `` leaves, seeds, flowers, fruits ''), sprouts, morello cherries `` fruits '', moroheiya (burlap), Yakawamurasakiimo, yakuchi (masuchi), cornflowers (cornflowers), cornflower mint, yacon, yashabushi (arrow wheel) or himeyashabushi or giant yashabushi `` fruit, peel, fruit ear '',
Willow willows, yatsude (octagonal gold plate), mistletoe (willow parasitism), willows (kawayanagi, tachiyanagi, weeping willow, akameanagi, pussy willow, dog puppy willow, kinuyanagi, koryanagi, unryuyanagi, miyanagiyanagi, yamanagiyanagi, yamayanagi, yamayanaginagi, yamayanagi, yamayanaginagi, yamanaginagi, yamanaginagi, yamanaginagi, yamanaginagi, yamanaginagi, yamanaginagi, yamanaginagi, yamanaginagi, yamanaginagi, yamanaginagi, yamanaginagi, yamanaginagi, yamanaginagi, yamanaginagi, yamanaginagi, yamanaginagi, yamanaginagi) , Willow litter "leaves, stems", horseshoe,
Yabu Koji (purple gold beef), Yabu cigarette (crane squirrel, Tenmeisei), pokeweed (commercial land), yamanoki (yamaharu), yamamomo (yang plum skin), yamamogi, eucalyptus, yukinoshita (tiger ear grass), yucca or flevifolia, yuzu "Fruit", lily, yogurusa, mugwort (Aiba), ligan (Rainuma), lime "fruit", rye, lakanka "fruit", raspberry "leaf,
Fruits, Latania (Crameria), Rakkyo or Shallot (Shiroi), Lavender, Longan (Longan flesh), Agave (Aono agave, Fukurin Agave), Ryokucha (green tea), Apple `` Fruits, seeds,
`` Leaves, roots '', gentian, rubus or suavisimus,
Ganoderma litchi (Lichi, Lichi nucleus), lettuce (Chisha), red currant "fruit", lemon "fruit", lemongrass, forsythia or Chinese forsythia (forsythia), astragalus, coconut palm, lokon (reed, azuki), loganberry Examples include "fruit", rosemary (mannen wax), rose hip (nobara), wasabi, and valerobium (chiyu).

In addition, other seawaters, for example, seawater salt, dried seawater, inorganic salts obtained from the Dead Sea or the Atlantic or Pacific Ocean (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud or mud (fango) Species, for example, sea mud or mud of various places such as Italian Fango, German Fango, Eifel Fango, Freiburg Fango, etc.
Silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, magnesium oxide, calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese,
Arsenic, water).

Examples of the seaweeds include seaweeds [green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Aonori (Usubaa nori, Susiaonori, Hiraaonori, Bouaonori, Hosoedaoonori), and seaweed [brown alga: Kombu algae: , Resilicon b, hosomekobu, mishitishibu), seaweed, hirome, blue seaweed, giant kelp (macrocystis pirifella, macrocystis integrifolia, neocitys luetokeana), hijiki, hibamata), seaweed [red alga: hijirimen, maxa] Tengsa),
Hirakusa, Omikusa, Obakusa, Katao Bakusa, Yatabegusa, Yuikiri, Shimanegusa, Tosakanori, Togekirinsai, Akumasakirinsai, Kirinsai, Zyakushinkirinsai, Tsunomata, Obatsunomata, Tochaka , Ibotsunomata, Yaleus Banori, Kagius Banori,
Sujiusbanori, haiusbanori, akaminominori] and the like.

Other algae such as green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Kwanomimo: Kwanomimo, Volvox: Ohikawawari, Volvox, Palmera, Yotsumemo, Spirogyra: Hizaori, Spirogyra, Tulsimidro, Hibimidro: Hibimidoro, Aosa: Anaoasa, Amiosa, Nagaosa, Kawanori: Kawanori,
Genus Fricciella, genus genus: medusa, asiaticum gutta, kawashiogusa, marimo, baronia: tamagobaronia, tamabaronia, tamagata: tamagatamo, iwata: husaiwata, surichogita, heraiwata, kurokita, kurokita, kurokita Sakibuchimil, Nagamir, Hiramil, and Casanori:
Casanori, Jusmo: Fusuzumo, Tamazumi,
Species of the genus Mizudzumo, Genus Genus, Genus Colecaet, Genus Genus genus, Species of the genus Cyperaceae: Species of the genus Cyperaceae, the genus of the spider genus;

Cyanobacteria (Genus Lynx: L., L., L., L .;
(Hassay, Yuremo genus, Racenmo (Spirulina) genus: Spirulina, Trichodesmium (Aiacacio) genus, etc.).

Brown algae (Piraella spp .: Piraella spp., Shiomidoro spp.
Genus Isogawara: Isogawara, Kuroshira Genus: Gunsenkurogashira, Kashirazaki Genus: Kashirazaki, Muchimo Genus: Muchimo, Hiramutimo, Kebelligsa, Amidixa Genus: Amidigsa, Sakibiroamiji, Sanadagusa: Sanadagusa, Fukurinamiji, Common Genus Artemisia spp.
Genus genus: genus pine, genus genus: genus genus: genus genus, genus okinawa, genus okinawazuku, genus phlebosum, genus phlebosum, genus phlebosum: genus futomozuku, genus genus moss: genus genus: genus kuromo, genus moss, genus genus Ishige, Irolo, Icmegasa: Ichimegasa, Keyari: Keyari, Umibos: Umibos, Urushigusa: Ursigusa,
Betula serrata, Tobacco moss, Laminaria spp. Genus Kagomenori: Kagomenori, Muraridori: Murachidori, Shakuganusa: Shakuganusa,
Genus Genus: Genus Genus, Genus Genus: Genus Genus: Genus Genus, Genus Kayamonori, Genus Genus: Genus Genus: Genus Filiformis, Genus Turmo: Genus Tsurumo, Genus Anana: Genus Anastasia, Genus Suzume: Genus Musicum, Genus Missiscoma, Genus L. , Oni-kombu, Gohei-kombu, Naga-kombu, Pea-kombu, Ochimitsu-kombu, Toro-kombu genus: Toro-kombu, Antokume genus: Antokume, Kajime genus: Kajime, Tsuruarame, Kurome, Kikuishicombu genus: Kikuishikombu, Nejirekombu: Nekorecome genus, Kuroshimae : Nekoshikambu,
Alame: Alame, Ainuwakame: Ainuwakame, Chigaiso, Oniwakameezoishige: Ezoishige, Yabanemoku: Yanemoku, Rapamoku: Rapamoku, Jiromoku: Uganomok, Jiromoku, Hiemomoku, Hokumoku, Tamamomoku, Tamamomoku Akamoku, Shidamoku, Hondawara, Nejimoku, Narasamo, Mametarawara, Tatsukuri, Yasumatamoku, Umitrano, Oobamoku, Fushizushimoku, Hahakimoku, Togemoku, Jolemoku, Saw-moku, Obanoki-mimoku, Genus genus:
Bulukimo, genus cyno (Kayamonoli etc.).

Red algae (Russula genus: Rush genus, Funorinoshiki, Amanori genus: Asakusanori, Susabinori,
Genus Upirinori, Onimanori, Tasa, Filitasa, Benitasa, Rhodocorton: Milnoveni, Akebonomozuku: Akebonomozuku, Konahada: Haikonahada, Yogorekonahada, Aokonahada, Sea Species: Acer genus, Acer genus : Larch, Fusanori: Fusanori, Nisefusanori: Nisefusanori,
Sodegarami: Sodegarami, rattles: rattle, rattle rattle, hirohatamatamaki genus: hirohatamaitadaki, tamamaidaki genus: tamaitadaki, kagikenori genus: kaginori, kakenenori, tengusa: himetenugosa, haitenugosa, haitenugosa, haitenugusaugou, haitenuganugou, haitenuganugou, haitenugusaugou Genus: hibiroudo, himehibiroudo,
Genus Isommedogi: Genus Isomomedo, Genus genus: Genus medusa, Ryumonsou: Ryumonsou, Hera Ryumon, Nisekarekususa: Genus Nisekaregusa, Okitsubara: Obaokitsubara, Akaba: Akaba, Malvaacaba, Naminohana genus
Naminohana, coral genus: rattle, mushroom, shiogosagoromo, iwanokawa: ezukiiwanokawa, kainokawa: kainokawa, kaninote: kaninote, coral genus: coral, coral, mosquito mosquito , Tambanori, slippery,
Genus Isonohana: Nuracusa, Cronulla, Stinging genus, Hirakintoki: Hirakintoki, Matagawa: Matabou, Kintoki: Chabokintoki, Kintoki, Matsunori, Komenori, Tosakamatsu, Aedes pine, Kakraitus: Genus Odonata, Genus Larva Genus Nagaobane, Funori: Hanafunori, Fukuronori, Makuhnori, Karenikusa: Kalexigusa, Tosakamomoki: Hosobanotokamodoki, Hirohanotosakamakiki, Yatsudegatomodoka, Kurosakacamodoki, Nezashinodoka, Nezashinotokada : Ezotosaka, Tsukasanori: Enashikarimenia, Otsukasanori, Hanagatakarimenia, Honooka: Hounoo, Hikagenoito: Hikagenoito, Usuginu, Nikuhono: Nikuho Noo, Benisunago genera: Benisunago, Susukakebeni genera: Susukakebeni, Okamuragusa genera:
Yamadakusa, Mirin genus: Mirin, Hosobamilin, Tosakanori genus: Kikutosaka, Ezonameshi: Ezonameshi, Isomokka genus: Isomocca, Yukari genus: Yukari, Hosoyukari, Ibaranori genus: Ibaranori, Saidaibara, Tachiibara, Kagiibanodan, Kijinotho genus Isanthus, Atsubanori: Atsubanori,
Ogonori: Ogonori, Tsurushiramo, Shiramo, Ogogonori, Mizookonori, Kabanori, Tengusamodoki: Hachijotengusamodoki, Fushikrenori: Fushikenori, Namiiwatake: Namiiwatake, Spiraea:
Sponge and genus: ssp.
Genus: crocodile, crocodile, crocodile, lotus spp .: lotus spp. The genus Taogosou: Taoyanagisou, Hanasakura, Shirotsunagi: Skullconi, Shikokonori, Hananoeda: Hanaeda, Hiratagoat: Hiratagoat, Darusu: Dars, Masagoshibari, Anadars, Weedbugsa: Uebaguni, Benifukunori
Species of sarcophagi: sarcophagia, scorpion, scorpion, scorpion genus: syllabus, smelt, ssp. Family: Habutaenori, Konohanori, Suzuronori, Usbeni: Usbeni, Hasuginu: Hasuginu, Nagakonohanori: Na

The axle algae (Charus spider, Shiratamamo,
Hossiturimo: Hossiturimo, Lychnotanus genus, Hessian moss: Himejirasamo, Chaboshiramo, Tripera, etc., and yellow algae (Hikarimo: Hikarimo etc.).

Examples of the material derived from animal raw materials include a cockscomb extract, a cow or human placenta extract, a pig or cow extract from the stomach, duodenum or intestine or a decomposition product thereof, and a pig or cow spleen extract. Product or its degradation product, extract of pig or cow brain tissue, water-soluble collagen, collagen derivative such as acylated collagen, collagen hydrolyzate, elastin, elastin hydrolyzate, water-soluble elastin derivative, keratin and its decomposition product Or a derivative thereof, a silk protein and its degradation product or a derivative thereof, a pig or bovine blood cell protein degradation product (globin peptide), a pig or bovine hemoglobin degradation product (hemin, hematin, heme, protohem, heme iron, etc.),
Cow milk, casein and its decomposition products or their derivatives, skim milk powder and its decomposition products or their derivatives, lactoferrin or its decomposition products, chicken egg components, fish meat decomposition products and the like.

(14) Microbial culture metabolite Yeast metabolite, yeast extract, natto metabolite, natto extract, rice ferment extract, rice bran ferment extract, euglena extract, lactic acid fermentation of raw milk or skim milk powder, and trehalose Or a derivative thereof.

(15) α-hydroxy acids glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(16) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

(17) UV absorber / blocker Benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid Sodium, dihydroxydimethoxybenzophenone, sodium dihydroxydimethoxybenzophenone-sulfonate, 2,4
-Dihydroxybenzophenone, tetrahydroxybenzophenone, etc.), p-aminobenzoic acid derivatives (such as paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, octyl paradimethylaminobenzoate), methoxycinnamic acid Derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate, octyl paramethoxycinnamate, 2-ethoxyethyl paramethoxycinnamate, sodium paramethoxycinnamate, potassium paramethoxycinnamate,
Diparamethoxycinnamate mono-2-ethylhexanoate glyceryl, etc.), salicylic acid derivatives (octyl salicylate, salicylphenyl, homomenthyl salicylate, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate, methyl salicylate, etc.), anthranilic acid derivatives (methyl anthranilate) Urocanic acid derivatives (urocanic acid, ethyl urocanate, etc.), coumarin derivatives, amino acid compounds,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin B6 derivatives, umbelliferone, esculin,
Benzyl cinnamate, sinoxate, oxybenzone, dioxybenzone, octabenzone, slisobenzone, benzoresorcinol, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, escalol, zinc oxide, talc, kaolin and the like.

(18) Whitening agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin C or a derivative thereof, vitamin E or a derivative thereof, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein,
Berberine, placenta extract, etc.

(19) Tyrosinase activity inhibitor Vitamin C acid or its derivative, hydroquinone or its derivative (hydroquinone benzyl ether), kojic acid or its derivative, vitamin E or its derivative, N-acetyltyrosine or its derivative, glutathione, Hydrogen oxide, zinc peroxide, hydroquinone monobenzyl ether, placenta extract, silk extract, plant extract (mulberry, squid, waremokou, clara, mugwort, honeysuckle, yellowfin, dokudami, matsuhodo, barley, hydrangea, hops, hawthorn, Eucalyptus, Yarrow, Altea, Keihi, Mankeishi, Hamamelis, Yamaguwa,
Prolonged life grass, bellflower, toshishi, spermatozoa, sun dried, mahuang, senkyu, dokkatsu, psycho, bohu, hamabofu, ougon, peony, peonies, genoshoko, kakkon, licorice, five times child, aloe, shoma, safflower, green tea, tea, Asenyaku).

(20) Melanin pigment reduction / decomposition substance Phenylmercury hexachlorophene, mercuric oxide, mercurous chloride, aqueous hydrogen peroxide, zinc peroxide, hydroquinone or a derivative thereof (hydroquinone benzyl ether) and the like.

(21) Turnover promoting action / cell activator hydroquinone, lactic acid bacteria extract, placenta extract, reishi extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (Aloe, Ogon, Horsetail, Gentian, Burdock,
Sicon, carrot, hamamelis, hop, yokunin,
Odrich, Assemble, Touki, Calendula officinalis, Amacha, Hypericum perforatum, Cucumber, Periwinkle,
Rosemary, parsley).

(22) Astringent Succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, aluminum potassium sulfate, resorcinol, ferric chloride, tannic acid (including catechin compound) Such.

(23) Active oxygen scavenger Superoxide dismutase (SOD), catalase, glutathione peroxidase and the like.

(24) Antioxidant Ascorbic acid or ascorbic acid derivative or a salt thereof, stearic acid ester, tocopherol or tocotrienol or a derivative thereof, nordihydroguaseletenic acid, butylhydroxytoluene (BHT), butylhydroxyanisole ( BHA), hydroxytyrosol, parahydroxyanisole, propyl gallate, sesamol, sesamolin, gossypol and the like.

(25) Lipid peroxide production inhibitor β-carotene, plant extract (cultured cells of sesame, amateur,
Hypericum, Hamamelis, Clove, Melissa, Emmeso, Birch, Sage, Rosemary, Minami Amami,
Ages, ginkgo, green tea) etc.

(26) Anti-inflammatory agent ictamole, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor, dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizin Acid or a salt thereof, glycyrrhetinic acid or a salt thereof, licorice extract, sicon extract, age extract and the like.

(27) Antibacterial / bactericidal / disinfectant Acrynol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof, alkyl diaminoethyl glycine chloride solution, triclosan, sodium hypochlorite, Chloramine T, salad powder, iodine compound,
Iodoform, sorbic acid or a salt thereof, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p-chlorophenol, p-chloro-m-xylenol, p-chloro-m
-Cresol, thymol, phenethyl alcohol, o-
Phenylphenol, Irgasan CH3565, halocarban, hexachlorophen, chlorohexidine, ethanol, methanol, isopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, phenoxyethanol, chlorobutanol, isopropylmethylphenol, nonionic surfactant (polyoxyethylene lauryl ether , Polyoxyethylene nonyl phenyl ether, polyoxyethylene octyl phenyl ether, etc.), amphoteric surfactants, anionic surfactants (sodium lauryl sulfate, lauroyl sarcosine potassium, etc.), cationic surfactants (cetyltrimethylammonium bromide, benza chloride) Ruconium, benzethonium chloride, methylrosaniline chloride), formaldehyde, hexamine, Reliant Green, Malachite Green, Crystal Violet, Jarmal, Photosensitizer No. 101, Photosensitizer No. 201, Photosensitizer No. 401, N-long-chain acyl basic amino acid derivatives and acid addition salts thereof, zinc oxide, hinokitiol, kudin and the like.

(28) Humidifier Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin or chitosan derivative, pyrrolidone carboxylic acid or its salt, lactic acid Sodium, Mini Sasanishiki extract, Bacillus natto metabolite, Natto extract, etc.

(29) Elastase activity inhibitor diisopropyl fluorophosphate, plant extracts (Ougon, Hypericum perforatum, Clara, mulberry leaves, calyx, gennoshoko, comfrey, sage, western elderberry, bollige, buttonpi), seaweed extract and the like.

(30) Hair Agent Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, bromate Sodium, thioglycolic acid and the like.

(31) Antiandrogens Estrogen (eg, estrone, estradiol, ethinylestradiol), isoflavone, oxendrone, etc.

(32) Peripheral vascular blood flow promoter Vitamin E or a derivative thereof, assembly extract, garlic extract, carrot extract, aloe extract, gentian extract, touki extract, cepharanthin, carpronium chloride, minoxidil and the like.

(33) Topical stimulants Pepper tincture, vanillamide nonylate, tincture tincture, tincture tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(34) Metabolic activators Photosensitizer 301, hinokitiol, pantothenic acid or derivatives thereof, allantoin, placental extract, biotin, glyceride pentadecanoate and the like.

(35) Antiseborrheic agents pyridoxine or its derivatives, sulfur, vitamin B6 and the like.

(36) Keratolytic agent Resorcin, salicylic acid, urea, lactic acid and the like.

(37) Oxidizing agent Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, pyrroline peroxide Sodium acid, sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract and the like.

(38) Hair remover strontium sulfate, sodium sulfide, barium sulfide,
Inorganic reducing agents such as calcium sulfide; thioglycolic acid or salts thereof (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate, strontium thioglycolate) and the like.

(39) Hair swelling agent Ethanolamine, urea, guanidine and the like.

(40) Dyes 5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-
Amino-4-methylaminoanthraquinone, 3,3'-
Iminodiphenol, 2,4-diaminophenoxyethanol hydrochloride, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, meta-hydrochloride Phenylenediamine, orthoaminophenol, N-phenylparaphenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene
3,4-diamine, nitroparaphenylenediamine, paraaminophenol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol, picramic acid, sodium piclaminate, N,
N'-bis (4-aminophenyl) -2,5-diamino-1,4-quinonediimine, 5- (2-hydroxyethylamino) -2-methylphenol, N-phenylparaphenylenediamine, metaaminophenol, meta Phenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate, orthoaminophenol sulfate, orthochloroparaphenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, sulfuric acid Toluene-2,5-
Diamine, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthophenylenediamine sulfate, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol, diphenylamine , Α-naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcinol, tannic acid, 2-hydroxy-5-nitro-2 ', 4'-diaminoazobenzene-
Sodium 5'-sulfonate, hematein and the like.

(41) Flavor Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guayakwood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, Coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil, cedar essential oil, spearmint essential oil, western Mentha essential oil, Greater Fengxiang essential oil, Tuberose essential oil, T-shaped essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batturi essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petit glen essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemon grass essential oil, Lemon essential oil, Rosemary Vegetable flavors such as essential oils and Japanese peppermint essential oils, and other synthetic flavors.

(42) Dyes / colorants Red cabbage dye, red rice dye, madder dye, anato dye, squid dye, turmeric dye, ende dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

In addition, hormones, sequestering agents,
pH adjuster, chelating agent, antiseptic / antibiostatic agent, cooling agent, stabilizer, emulsifier, animal / vegetable protein or its degradation product, animal / vegetable polysaccharide or its degradation product, animal / vegetable glycoprotein or Decomposed products, anti-inflammatory and anti-allergic agents, wound treatment agents,
Bubble / foaming agents, thickeners, oral agents, deodorants / deodorants, bittering agents, seasonings, enzymes, etc. are used. Additional or synergistic improvements can be expected.

The melanin production inhibitor, active oxygen scavenger or skin external preparation or bath preparation of the present invention may have any dosage form, such as capsules, powders, granules, solids, liquids, gels, bubbles,
It can be in the form of an emulsion, cream, ointment, sheet or the like.

More specifically, for example, basic preparations such as medicinal preparations for external use, lotions, emulsions, creams, ointments, lotions, oils, and packs, facial cleansers and skin cleansers, depilatory agents, depilatory agents, aftershave lotions , Pre-show lotion, shaving cream, shampoo, rinse,
Hair treatments, hair styling agents, perm agents, hair tonics, hair dyes, hair growth and hair growth products, etc., makeup cosmetics such as foundation, lipstick, blusher, eyeshadow, eyeliner, mascara, perfumes, bath agents , Other, toothpaste, mouth freshener / gargle, deodorant,
Various products such as deodorant, sanitary cotton, wet tissue, etc. can be used, and can be blended into general foods and drinks.

The method of adding to the melanin formation inhibitor, active oxygen scavenger, skin external preparation or bath agent of the present invention may be added in advance or during the production, What is necessary is just to select suitably considering a property.

[0076]

The present invention will be described below with reference to production examples, test examples, and prescription examples, but the present invention is not limited to these examples.

(Production Example 1) Olive leaves (100 g) were purified water (1
kg) for about 5 hours, extract (about 80 ° C), filter and extract
(Dry solids content: about 0.3-2.5% by weight).

(Production Example 2) 30% 1,3-
It is immersed in a butylene glycol solution (1 kg) or a 30% ethanol solution (1 kg), extracted at room temperature for 5 days and nights, and then filtered to obtain about 1 kg of an extract (dry solid content: about 0.3 to 2.5% by weight).

(Production Example 3) Olive leaves (100 g) in 70% ethanol solution (1 kg) or 50% 1,3-butylene glycol solution
(1 kg), or immersed in 60% propylene glycol solution (1 kg), or purified water (1 kg), extracted at room temperature for 5 days and nights, filtered and extracted (dry solid content: about 0.3 to 3.0% by weight) About 1kg
obtain.

(Test 1) Melanin production inhibitory test Melanin production inhibitory effect was evaluated using B16 melanoma cells.

[Test Method and Evaluation Method] B16 melanoma cells pre-cultured for 24 hours were transferred to a fresh MEM medium (5 mL).
A sample (0.5% of the medium volume) is added thereto, and the cells are cultured for 3 days. Next, the cultured cells were treated with trypsin, and dissolved by heating with a 1N-NaOH solution (1 mL) containing 10% DMSO.
The absorbance at 0 nm is measured. Note that distilled water was used as a blank in place of the sample, and cells to which the sample was added at the same time were subjected to MTT reduction (Tim Mosmann; Journal of Immunology).
The activity was measured by logical methods p55-63 (1983)), and the MTT reduction activity of the sample-loaded cells was calculated using the MTT reduction activity of blank cells as one unit. The amount of melanin in the sample was calculated as absorbance / MTT reduction activity (1 unit), and the inhibition rate (%) against the amount of melanin produced by blank cells was calculated.
Was determined by Equation 1. The results are shown in FIG.

"Sample" The sample of the melanin production inhibitory action test was the extract (solid content: 0.5%) obtained in the production example of the present invention.
Was used for the test. As a comparative sample, arbutin (manufactured by Sigma) was prepared under the same conditions and tested.

<Equation 1> Melanin production inhibition rate (%) = [1− (melanin amount of sample / amount of blank melanin)] × 100

(Test Results) As shown in FIG. 1, the production of melanin was significantly suppressed in the sample-added system, and it was confirmed that the olive leaf extract of the present invention had a high inhibitory effect on melanin production. Also, compared to the known active ingredient arbutin,
Since almost the same or better effects were observed,
It is considered useful as a whitening agent.

(Test 2) Elimination of active oxygen (SOD-like activity)
Action test In recent years, many diseases and diseases induced by active oxygen have been reported, and removal and elimination of active oxygen (SOD-like activity)
It has been pointed out that this can lead to prevention of organizational disability. In this test, the extract obtained in the production example was tested for its ability to eliminate active oxygen generated by a xanthine-xanthine oxidase system using the NBT method of the Imana et al. Co., Ltd.).

[Test Method and Evaluation Method]
2.5 mL of M sodium carbonate buffer (pH = 10.2), 0.75 mM nitro blue tetrazolium (NBT), 3.0 mM xanthine, 3.0 mM disodium ethylenediaminetetraacetate, 0.15 W
/ V% bovine serum albumin and 0.1 mL each of a sample solution (the solvent for the control) were added, and the mixture was kept at 25 ° C. for 10 minutes. The aqueous solution of xanthine oxidase (the change in the absorbance of NBT in the control was about
0.1 mL was added, and the absorbance at 560 nm was measured. A sample containing purified water instead of a sample was used as a control, and a blank containing a reaction stop solution was set for each sample and control before adding xanthine oxidase. %)
I asked. The results are shown in FIG.

"Sample" The sample used was the extract (solid content: adjusted to 0.10% and 0.05%) obtained in the production example of the present invention.
Tested. As a comparative sample, a Tonaka leaf extract was prepared under the same conditions and used for the test.

<Equation 2> Active oxygen elimination rate (%) = [1− (sample OD value−blank (sample) OD value) / (control OD value−blank (control) OD value)] ×
100

(Test Results) As shown in FIG. 2, it was confirmed that the olive leaf extract of the present invention significantly suppressed the production of active oxygen and had a high active oxygen eliminating action. In addition, since it has a higher active oxygen scavenging effect than the Tochu leaf extract, which is a known active ingredient, it is considered to be useful as an active oxygen scavenger.

(Test 3) Safety test (1) Primary skin irritation test Various plant extracts obtained in Production Examples 1 to 3 were prepared with purified water so that the dry solid content concentration was 1.0%. Japanese white rabbit with shaved back (female, 3 per group, weight around 2.3kg)
Was applied to the skin. At 24, 48, and 72 hours after application, evaluation was performed using erythema and edema as indices based on the criteria of Draize. The results confirmed that all animals did not show any erythema and edema and were safe with respect to primary skin irritation.

(Test 4) Safety Test (2) Skin Cumulative Irritation Test Various plant extracts obtained in Production Examples 1 to 3 were prepared with purified water so that the dry solid content concentration was 1.0%. Hartley guinea pig with shaved back (female, 3 per group, weight 33)
0.5 g / animal) was applied to the skin (about 0 g) once a day, 5 times a week. Application was performed over two weeks and shaving was performed on the last application day of each week. Evaluation was performed using erythema and edema as indices based on the criteria of Draize on each application day and the day after the last application day. The results confirmed that all animals did not show any erythema and edema for 2 weeks and were safe with respect to cumulative skin irritation.

(Test 5) Safety test (3) Single dose toxicity test The various plant extracts obtained in Production Examples 1 to 3 were adjusted with purified water to a concentration of 12.5%. kg quantity,
Before the test, ddy mice (male and female, 5 animals per group, 5 weeks old) which had been fasted for 4 hours were orally administered, and the appearance and degree of toxic symptoms were observed over time. As a result, no abnormality was observed in all the mice for 14 days, and it was confirmed that there was no abnormality in the dissection result. Therefore, LD50 is 2,000mg / k
g was considered to be a safe substance.

(Prescription example) Production of melanin production inhibitor, active oxygen scavenger, external preparation for skin or bath preparation In accordance with the above evaluation results, the following prescription examples are shown. It was produced by a conventional method, and only the compounding amount is shown.

(Formulation Example 1) Emulsion weight% 1. Squalane 8.0 2. Jojoba oil 7.0 3. Cetyl alcohol 1.5 4. Glycerin monostearate 2.0 5. Polyoxyethylene (20) cetyl ether 3.0 6. Polyoxyethylene (20 ) Soorbitan monooleate 2.0 7. 1,3-butylene glycol 1.0 8. Glycerin 2.0 9. Olive leaf 50% 1,3-butylene glycol extract 5.0 10. Kuzu 50% 1,3-butylene glycol extract 3.0 10 Preservative (Ethyl paraoxybenzoate) qs 11. perfume qs 12. purified water 100

(Formulation Example 2) Flexible lotion% by weight 1. Glycerin 5.0 2. 1,3-butylene glycol 5.0 3. Polyoxyethylene sorbitan monolaurate (20E.O) 1.0 4. Ethanol 15.0 5. Olive leaf 50 % Ethanol extract 4.0 6. Calendula 45% 1,3-butylene glycol extract 2.0 7. Vitamin C derivative 0.05 8. Arbutin 0.01 9. Antibacterial and preservative (thiamine lauryl sulfate) qs 10. Flavor qs 11. Purification Water 100 and the rest

(Formulation Example 3) Astringent lotion weight% 1. Sorvitol 2.0 2. POE (20) oleyl alcohol 1.0 3. Ethanol 15.0 5. Olive leaf 20% 1,3-butylene glycol extract 3.0 6. Novara 50% Ethanol extract 1.0 7. Time 50% 1,3-butylene glycol extract 1.0 8. Allantoin 0.2 9. Astringent (zinc sulfocarbonate) 0.2 10. Antibacterial and preservative (thiamine lauryl sulfate) qs 11. Flavor qs 12 . Residue with 100 purified water

(Formulation Example 4) Two-layer lotion (liquid / liquid type)% by weight 1. Squalane 8.0 2. POE sorbitan tetraoleate 0.3 3. Sorbit 1.0 4. Ethanol 15.0 5. Olive leaf 30% propylene glycol Extract 2.0 6. Dutch mustard 50% ethanol extract 1.0 7. Ascorbic acid 0.2 8. Astringent (sodium sulfocarbonate) 0.2 9. Preservative (phenoxyethanol) qs 10. Fragrance qs 11. Purified water 100

Formulation Example 5 Carmine Lotion (Liquid / Solid) Weight% 1. Glycerin 3.0 2. 1,3-butylene glycol 5.0 3. Ethanol 15.0 4. Iron oxide (Bengara) 0.2 5. Zinc oxide 0.5 6. Kaolin 2.0 7. Olive leaf 60% propylene glycol extract 5.0 8. Placenta extract 1.0 9. Camphor 0.2 10. Lactic acid or glycolic acid 0.03 11. Preservative (photosensitizer 201) qs 12. fragrance qs 13. Purified water 100 Residue to do

(Formulation Example 6) Peel-off pack weight% 1. Glycerin 5.0 2. Propylene glycol 4.0 3. Polyvinyl alcohol 15.0 4. Ethanol 8.0 5. Polyoxyethylene glycol 1.0 6. Pantothenyl ethyl ether 1.0 7. Olive leaf 50% Ethanol extract 1.0 8. Seaweed 50% 1,3-butylene glycol extract 1.0 9. Appropriate amount of flavoring agent 10. Appropriate preservative (paraoxybenzoate) 11. Purified water 100

(Formulation Example 7) Cream weight% 1. Salicone beeswax 11.0 2. Liquid paraffin 22.0 3. Lanolin 10.0 4. Olive oil 15.0 5. Borax 0.5 6. Olive leaf 30% 1,3-butylene glycol extract 5.0 7 Liquid shea butter 1.0 8. Hawthorn 50% 1,3-butylene glycol extract 2.0 9. Preservative (acrinol) 0.1 10. Appropriate perfume 11. Purified water 100

(Formulation Example 8) Face Wash Cream% by Weight 1. Myristic acid 25.0 2. Stearic acid 5.0 3. Tallow fatty acid 5.0 4. Propylene glycol 10.0 5. Potassium hydroxide 6.0 6. Coconut oil fatty acid diethanolamide 6.0 7. Olive leaf 50% 1,3-butylene glycol extract 2.0 8. Honeysuckle 30% 1,3-butylene glycol extract 2.0 9. Dipotassium glycyrrhizinate 1.0 10. Lactoferrin solution 0.1 11. Preservative (salicylic acid) 0.1 12. Fragrance Appropriate amount 13 . Residue with 100 purified water

(Formulation Example 9) Body soap weight% 1. Potassium laurate 15.0 2. Potassium myristate 5.0 3. Propylene glycol 5.0 4. Olive leaf 80% 1,3-butylene glycol extract 1.5 5. Loquat leaf 50% 1,3-butylene glycol extract 1.0 6. Common mint 30% ethanol extract 0.5 7. Bodige 45% 1,3-butylene glycol extract 0.5 8. Polyethylene powder 0.5 9. Soybean 30% ethanol extract 0.5 10. Amino acid (Glycine, glutamine, etc.) 0.5 11. Preservative (undecylenic acid, phenol) appropriate amount 12. pH adjuster appropriate amount 13. Purified water 100 residue

(Formulation Example 10) Sunscreen cosmetics (O / W type)% by weight 1. Oxybenzone 2.0 2. Octyl paramethoxycinnamate 5.0 3. Squalane 10.0 4. Vaseline 5.0 5. Stearyl alcohol 3.0 6. Stearic acid 3.0 7. Glyceryl monostearate 2.0 8. Ethyl polyacrylate 1.0 9. 1,3-butylene glycol 6.0 10. Disodium edetate 0.1 11. Triethanolamine 99% 1.0 12. Titanium dioxide 5.0 13. Olive leaf 30% 1 , 3-Butylene glycol extract 2.5 14. Ogon 50% ethanol extract 1.0 15. Sicon 30% 1,3-butylene glycol extract 0.5 16. Preservative (paraben) qs 17. Purified water 100 residue

(Formulation Example 11) Sunscreen cosmetics (oil type)% by weight 1. Liquid paraffin 70.0 2. Ethyl p-aminobenzoate 1.0 3. Tetrahydroxybenzophenone 1.0 4. Cetyl octanoate 25.0 5. Olive leaf 90% 1,3-butylene glycol extract 1.0 6. Rice germ oil 1.0 7. Adlay 50% 1,3-butylene glycol extract 1.0 8. Antioxidant (butylhydroxytoluene)

(Formulation Example 12) Shampoo wt% 1. Triethanolamine lauryl sulfate 5.0 2. Sodium polyoxyethylene lauryl ether sulfate 12.0 3. 1,3-butylene glycol 4.0 4. Diethanolamide laurate 2.0 5. Edetate di Sodium 0.1 6. Olive leaf 40% propylene glycol extract 2.0 7. Roman chamomile 50% 1,3-butylene glycol extract 1.0 8. Seaweed 50% 1,3-butylene glycol extract 2.0 9.4% hydroxypropyl chitosan Solution 2.0 10. Antibacterial / preservative (thiamine lauryl sulfate) qs 11. pH adjuster qs 12. Fragrance qs 13. Purified water 100 residue

(Formulation Example 13) Rinse weight% 1. Stearyltrimethylammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Olive leaf 50% 1,3-butylene glycol extraction Solution 1.0 6. Rosemary 50% 1,3-butylene glycol extract 1.0 7. Panax ginseng 50% ethanol extract 2.0 8. Hydrolyzed silk solution 1.0 9. Comfrey 50% ethanol extract 1.0 10. Antibacterial and preservative ( Benzalkonium chloride) Appropriate amount 11. Fragrance Appropriate amount 12. Purified water 100

(Formulation Example 14) Hair liquid weight% 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphate 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Olive leaf hot water extract 1.0 6. Soybean hot water extract 1.0 7. Assembly 60% ethanol extract 1.0 8. Chimpi 50% 1,3-butylene glycol extract 1.0 9. Keratin oxidative degradation solution 1.0 10. Appropriate amount of pH adjuster (citric acid) 11. Antibacterial Preservative (paraben, benzalkonium chloride) Appropriate amount 12. Perfume Appropriate amount 13. Purified water 100 residue

(Formulation Example 15) Hair tonic weight% 1. Ethanol 40.0 2. Ethyl oleate 1.0 3. Polyoxyethylene (40) hydrogenated castor oil 2.0 4. Olive leaf 50% 1,3-butylene glycol extract 1.05 Seaweed 10% 1,3-butylene glycol extract 2.0 6. Peony skin 50% ethanol extract 2.0 7. Hydroxypropyl β-1,3-glucan solution 1.0 8. Mucopolysaccharide (porcine gastric mucosa mucin) solution 1.0 9 Antibacterial and preservative (paraben) 0.1 10. Appropriate amount of fragrance 11. Residual with purified water 100

(Formulation Example 16) Granule bath preparation weight% 1. Sodium bicarbonate 58.0 2. Anhydrous sodium sulfate 30.0 3. Borax 3.0 4. Olive leaf 80% ethanol extracted powder 6.0 5. Peony 50% ethanol extracted powder 1.0 6 Fermented milk extract powder 2.0

(Formulation Example 17) Coating Protecting Agent Gauze or liniment cloth is impregnated with a 30% ethanol extract solution of olive leaves, a formulation solution containing an appropriate amount of antibiotics, anti-inflammatory and the like, and used as a clothing protecting agent attached to the wound. . Alternatively, an olive leaf 30% 1,3-butylene glycol extract may be directly applied locally and covered with gauze or the like.

(Test 6) Use effect test A use test was conducted in order to evaluate the effect when the external preparation for skin of the present invention was actually used. The condition is a person who suffers from spots, freckles, dark and black (34-41 years old, 8 people) and a person who suffers from dry skin or skin without gloss and tension (26-47 years old, 10 people), who are panelists every day. Prescription example 1 after washing face twice a night
A suitable amount of the milky lotion (test product) was applied to the left half of the face, and a control product obtained by removing the olive leaf 50% 1,3-butylene glycol extract from the milky lotion was applied to the right half of the face as a control. The evaluation was performed according to the following criteria. The results are shown in Tables 1 and 2, and the numbers in the tables represent the number of persons. No one complained of skin abnormalities during the use period.

"Stain, freckles, skin color improving effect" Efficacy: Blemish, freckles, and skin blackness were less noticeable (improved) than before. Slightly effective: spots, freckles, and skin darkness were slightly reduced. Ineffective: No change from before use.

[0113]

"Effect of improving skin quality" Efficacy: The gloss and firmness of the skin increased, and the dry skin quality was improved to a crisp skin. Somewhat effective: Skin gloss and tension increased slightly, and dry skin quality was slightly improved. Ineffective: No change from before use.

[0115]

(Test Results) As shown in Tables 1 and 2, the emulsion containing the olive leaf extract of the present invention significantly improved dry skin, provided gloss and firmness to the skin, and improved the appearance of spots and freckles. We confirmed that it was effective for reduction. In addition, there was a case that it was effective in reducing eczema.

(Test 7) Use effect test A use test was carried out to evaluate the effect when the external preparation for skin of the present invention was actually used. The conditions were as follows: 14 women (31 to 55 years old) suffering from rough hands were panelists, and the appropriate amount of the cream (test product) of Formulation Example 7 was applied to one of the left and right fingers twice daily, morning and night. Then, a cream obtained by removing the olive leaf 30% 1,3-butylene glycol extract from the cream was applied to the other finger. or,
The evaluation was performed according to the following criteria. The results are as shown in Table 3 and the numbers in the table represent the number of persons. No one complained of skin abnormalities during the use period.

"Effect of improving skin roughness" Effective: Hand roughness was improved. Slightly effective: Hand roughening was somewhat reduced. Ineffective: No change or deterioration before use.

[0119]

(Test Results) As shown in Table 3, it was confirmed that the cream containing the olive leaf extract of the present invention was effective in improving or reducing hand roughness.

(Test 8) Use effect test A use test was conducted in order to evaluate the effect when the bath agent of the present invention was actually used. The condition is after the bath, elbow,
People suffering from knees, heels, backs, etc. (35-65
Age, 10) as panelists, and for two weeks after the start of the test,
For the next two weeks, 30 g / bath water (200 g) of the bath preparation of Formulation Example 16 prepared as a control product except for the olive leaf 80% ethanol-extracted powder, and the granule bath preparation of Test Example 16 for the next two weeks.
L) (per time / day). The evaluation was performed according to the following criteria. The results are shown in Table 4 and the numbers in the table represent the number of persons. No one complained of skin abnormalities during the use period.

"Improvement effect on elbows, knees, heels, and back swelling" Effectiveness: Sickness disappeared (improved). Slightly effective: Kasatsuki was slightly reduced. Ineffective: No change from before use.

[0123]

[0124]

The melanin production inhibitor and active oxygen scavenger containing the olive leaf extract of the present invention as an active ingredient have been confirmed to have excellent melanin production inhibitory and active oxygen scavenging effects. In addition, the external preparation for skin or bath preparation containing them has an effect of improving spots, freckles, skin color, improving dry skin, eczema, rough skin, and giving a shiny or firm skin or hair.

By combining the melanin production inhibitor or active oxygen scavenger according to the present invention with the above-mentioned components and additives usually used in cosmetics and bath preparations, the effects of the present invention can be improved or Since new effects can be expected to be exhibited, it is expected that its use in skin external preparations and bath preparations will be limited.

[Brief description of the drawings]

FIG. 1 is a graph showing the melanin production inhibitory rate of the olive leaf extract of the present invention.

FIG. 2 is a view showing an active oxygen scavenging rate of an olive leaf extract of the present invention.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61K 7/50 A61K 7/50 31/00 617 31/00 617 639 639C 35/78 35/78 C // A61K 7/02 7/02 A 7/06 7/06 7/075 7/075 7/08 7/08 7/42 7/42 F term (reference) 4C083 AA072 AA082 AA111 AA122 AB032 AB052 AB152 AB232 AB242 AB312 AB352 AB442 AC012 AC022 AC072 AC102 AC122 AC132 AC172 AC182 AC212 AC242 AC252 AC302 AC342 AC352 AC392 AC432 AC442 AC472 AC482 AC532 AC542 AC552 AC582 AC642 AC682 AC692 AC782 AC792 AC902 AD022 AD042 AD092 AD112 AD312 AD322 AD392 AD442 AD452 AD512 AD532 AD642 CC51 CC01 CC04 CC33 CC39 DD33 EE12 EE13 EE16 4C088 AA12 AB12 AB15 AB16 AB26 AB38 AB51 AB58 AB59 AB61 AB62 AB64 AB67 AB77 AC05 AD13 BA09 BA10 CA05 CA08 MA22 MA28 MA63 NA14 ZA92

Claims (3)

[Claims] 1. A melanin production inhibitor comprising an olive leaf extract as an active ingredient. 2. An active oxygen scavenger comprising an olive leaf extract as an active ingredient. 3. An external preparation for skin or bath containing the melanin production inhibitor according to claim 1 or the active oxygen scavenger according to claim 2, which comprises an olive leaf extract as an active ingredient.