JP3449759B2 - Whitening cosmetics - Google Patents

Description

Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic having a skin whitening effect, and more particularly to a plant extract having a whitening effect based on an inhibitory action on melanin production as an active ingredient. It relates to blended whitening cosmetics. 2. Description of the Related Art Generally, it is considered that darkening, spots, freckles, etc. due to sunburn are caused by the formation of melanin which is a black-brown amorphous pigment. When stimulated, tyrosinase (tyrosine oxidase) present in the melanocytes of the skin is activated, and tyrosine, one of the amino acids constituting the protein, is oxidized and produced. [0003] Accordingly, since an effect of whitening skin is expected by suppressing the activity of tyrosinase involved in melanin production, it has been proposed to add a tyrosinase activity-inhibiting component to cosmetics. Conventionally, as whitening cosmetics having a whitening effect, as disclosed in JP-B-55-43443, "Whitening cosmetics" and JP-B-54-974, "Composition of crude drug extraction", ascorbic acid or In addition to those containing a derivative thereof, an external skin material containing arbutin (Japanese Patent Application Laid-Open No. 60-16906), a bleaching cosmetic containing kojic acid (Japanese Patent Publication No. 32-8100), a plant component ( JP-A-63-2913) or an animal component (JP-A-63-8913).
No. 312) is known as having a whitening effect. However, many of the above-mentioned conventional cosmetics do not have a sufficient whitening effect, and also have problems in safety to the skin such as insufficient storage stability and irritation. There were many. SUMMARY OF THE INVENTION [0006] The present invention solves the above-mentioned problems of the prior art, and has a novel skin whitening effect, and has a sufficient storage stability and high safety. The purpose is to provide whitening cosmetics. Means for Solving the Problems The inventors of the present invention have conducted intensive studies to solve the above-mentioned problems, and as a result, a plant obtained from Amor, Panicle, Buffalo Uri, Achote, and Guacol is a plant produced in Central America. The present inventors have found that the extract has a melanin-suppressing effect on melanoma cells, and have provided the present invention. [0008] That is, the present invention relates to a whitening cosmetic comprising an extract obtained from at least one plant selected from the group consisting of amor, spikelet, buffalo uri, achote and guacol. is there. The cosmetic of the present invention can be produced by the following method. First, Amor (scientific name: Stegnosperma halimifolium), Hobiyu (scientific name: Am)
aranthus candatus), buffalo cucumber (scientific name: Cucu)
rbita foetidissima), Achote (scientific name: Bixa orellaa)
na) and guayule (scientific name: Parthenium argentatum)
) Is dried by heating using an extraction solvent. In this case, as the extraction solvent, alcohols such as methanol, ethanol, propanol or isopropyl alcohol, water or a mixed solution thereof can be used.
It has been confirmed that an extraction efficiency of 1 hour at 50 ° C. using 0-70% aqueous alcohol is good. After the extraction, the extract is filtered to obtain an extract, and the extract is further concentrated under reduced pressure while heating at a temperature of 60 ° C. or less to dryness. In this case, the above-mentioned extract may be directly blended with the cosmetic. The extracts of the plants (amol, spikelet, buffalo uli, achote and guacol) thus obtained have a superior melanin production inhibitory activity at a lower concentration than the conventionally used ascorbic acid. It has been confirmed by the present inventors and others that a whitening cosmetic having a whitening effect can be obtained by incorporating this extract as an active ingredient in an amount of 0.01 to 5.0%. [0013] As the dried product of the plant that can be used in the present invention, each part of these plants such as fruit, leaves, stems and roots can be used. Hereinafter, the present invention will be described in detail with reference to Examples, but the scope of the present invention is not limited thereto. Embodiment 1 In this embodiment, an example of a method for extracting ear panicles will be described. First, about 100 g of the dried panicle was pulverized with a mixer, and the pulverized substance and 500 ml of 50% ethyl alcohol were placed in a flask and subjected to reflux extraction at 50 ° C. for 1 hour with stirring. After the extraction, this solution was subjected to suction filtration, and the obtained filtrate was concentrated under reduced pressure at 50 ° C. using an evaporator. Then, the obtained concentrated liquid was dried under reduced pressure to obtain 7.2 g of a brown powder. In the same manner as described above, dried amol, buffalo uri, achote, and guaco
14.4 g each as an extract from pulverized 0 g.
g, 15.3 g, 7.2 g and 8.4 g. Example 2 In this example, the amor obtained in Example 1
The melanin production inhibitory effect of each of the extract of panicle bilberry, buffalo uli, acochate, and guacol was measured.
First, B16 melanoma cells (B16FO, ATCC) which are malignant melanoma cells derived from mice that produce melanin
No. CRL-6322) and fetal bovine serum at a final concentration of 1
Were cultured in 0% and comprising as the added Eagle's MEM medium, to each well of a 6-well plate (FALCON Corp.), the culture medium was placed 6ml containing the cells at a concentration of ^{3 × 10 3 cell / ml,} CO 2 Incubator (5% CO ₂ , 3
(7 ° C) for 5 days. Next, this medium was replaced with a new Eagle MEM medium (6 ml) containing 0.03% theophylline (manufactured by SIGMA), and an appropriate amount of the sample solution (the extract obtained in Example 1) was added to each well. Was further cultured for 3 days. After completion of the culture, the medium was discarded, 1 ml of physiological saline was added to each well, and the cells adhered to the bottom of the well were suspended using a scraper to scrape the cells. Next, the cell suspension was transferred to a microcentrifuge tube (1.5 ml, manufactured by Eppendorf) using a pipette, and centrifuged (1,000 × g, 15 minutes).
did. On the other hand, as a control, sterilized water was added in place of the sample solution, and the same test was performed. In addition, 2% instead of the sample solution was used as an experimental group for comparing the whitening of cells.
L-ascorbic acid aqueous solution (a) 60 μl, (b) 1
50 μl and (c) 300 μl were added, and the same test was performed. Next, the degree of whiteness of the pelleted cells was visually compared to determine the melanin production inhibitory effect. In this case, the degree of whiteness of the cells in the control group (sterilized water-added group) was "-", and the degree of whiteness of the cells in the comparative group to which L-ascorbic acid was added was (a):
“+”, (B): “++”, (c): “++”, the degree of whiteness of the cells when the sample solution was added,
Which one of-, +, ++, and +++ it corresponds to was visually determined, and the strength of the melanin production inhibitory effect of the sample solution was determined in four steps, and the results are shown in Table 1. [Table 1] As is evident from the results shown in Table 1, each of the extract of panicle bilberry, buffalo uri, achote and guayule was L-ascorbic acid 5 at a concentration of 200 μg / ml.
A melanin production inhibitory action equivalent to 00 μg / ml was shown. Also, it was confirmed that the amor extract exhibited a melanin production inhibitory effect at a low concentration of 50 μg / ml equivalent to that of 1000 μg / ml L-ascorbic acid, and inhibited melanin production at a lower concentration than L-ascorbic acid. Was done. Example 3 In this example, an example of blending the extract of panicle biloba obtained in Example 1 with a whitening cosmetic will be described. (% By weight) Panicle 1.0 Glycerin 5.0 Polyoxyethylene sorbitan monolaurate 1.5 Ethanol 10.0 Appropriate amount of preservative, antioxidant Appropriate amount of dye Appropriate amount of purified water Remainder of the present invention EFFECTS As described above, the extracts of Central American plants Amol, Panicle, Buffalo Uri, Achote and Guayule have an inhibitory effect on melanin production in melanoma cells, and at least one of these extracts In addition to having an excellent skin whitening effect, the extracts of the present invention exhibit excellent skin whitening effect in a small amount and are highly safe because of low toxicity to cells. is there.

Claims (1)

(57) [Claims] [Claim 1] Amor, ear biyu, buffalo uri,
A whitening cosmetic comprising an extract obtained from at least one plant selected from the group consisting of achote and guayule.