JPH07118138A - Beautifying cosmetic - Google Patents

Abstract

PURPOSE:To obtain a cosmetic having excellent beautifying effect on skin, sufficient shelf stability and high safety to skin free from irritation. CONSTITUTION:This beautifying cosmetic comprises an extract from a plant such as Stegnospoerma halimifolium, Amarantus candatus, Cucurbita foetidissima, Bioxa orellana and Parthenium argentatum with a solvent and has raised beautifying effect without impairing safety to human body.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic having a skin whitening effect, and more particularly to a whitening cosmetic containing as an active ingredient a plant extract having a whitening effect based on a melanin production inhibitory effect.

[0002]

2. Description of the Related Art Generally, it is believed that dark skin, spots, freckles, etc. due to sunburn are caused by the production of melanin, which is a dark brown amorphous pigment, and this melanin causes external stimuli such as ultraviolet rays on the skin. Upon reception, tyrosinase (tyrosine oxidase) present in melanocytes of the skin is activated, and tyrosine, which is one of the protein-constituting amino acids, is oxidized and produced.

Therefore, since the effect of whitening the skin is expected by suppressing the activity of tyrosinase involved in melanin production, the incorporation of a tyrosinase activity suppressing component into cosmetics has been proposed.

Conventionally, as whitening cosmetics having a whitening effect, ascorbic acid or ascorbic acid as disclosed in JP-B-55-43443, "Whitening cosmetics" and JP-B-54-974, "Crude drug extract-containing composition". Besides the known ones containing the derivative, a skin external preparation containing arbutin (JP-A-60-16906), a bleaching cosmetic composition containing kojic acid (Japanese Patent Publication No. 32-8100), a plant component ( JP-A-63-2913) or animal components (JP-A-63-8)
It is known that cosmetics extracted from No. 312) have a whitening effect.

However, many of the above-mentioned conventional cosmetics do not have a sufficient whitening effect, and also have insufficient storage stability and irritation, which are problematic for skin safety. There were many.

[0006]

DISCLOSURE OF THE INVENTION The present invention solves the above-mentioned problems of the conventional techniques, has a superior skin whitening effect, and has a sufficient storage stability and high safety, which is a novel whitening cosmetic composition. The purpose is to provide.

[0007]

Means for Solving the Problems As a result of intensive studies by the present inventors in order to solve such problems, it was found that plant extracts obtained from Central American plants such as Amor, Panicle, Buffalo Uri, Achote and Guacol It was found that it has a melanin suppressing effect on melanoma cells, and was able to provide the present invention.

That is, the present invention relates to a whitening cosmetic characterized in that it contains an extract obtained from at least one plant selected from the group consisting of amor, panicle, buffalouri, achote and guacol. is there.

[0009]

The cosmetic of the present invention can be produced by the following method. First, Amor (Scientific name: Stegnosperma halimifolium), which is from Central America, and Earbill (Scientific name: Am
aranthus candatus), Buffalo Uri (scientific name: Cucu
rbita foetidissima), Achote (scientific name: Bixa orellaa
na) and guayule (scientific name: Parthenium argentatum
The dried product or dry pulverized product of 1) is extracted by heating with an extraction solvent.

In this case, as the extraction solvent, alcohols such as methanol, ethanol, propanol or isopropyl alcohol, water or a mixed solution thereof can be used. For example, the alcohol concentration is 2
It has been confirmed that extraction efficiency is good when extraction is performed at 50 ° C. for 1 hour using 0 to 70% hydrous alcohol.

After extraction, the extract is filtered to obtain an extract, which is then concentrated under reduced pressure while heating the extract at a temperature of 60 ° C. or lower to dryness, and the dry extract is recovered to prepare a cosmetic composition. In this case, the above extract may be directly blended with the cosmetic.

The thus obtained extracts of plants (amor, panicle, buffalouri, achote and guacol) have an excellent melanin production inhibitory action at a low concentration as compared with conventionally used ascorbic acid. It has been confirmed by the present inventors that the above effect is exhibited, and by adding 0.01 to 5.0% of this extract as an active ingredient, a whitening cosmetic having a whitening effect can be obtained.

As the dried plant product which can be used in the present invention, various parts such as fruits, leaves, stems and roots of these plants can be used.

The present invention will be described in detail below with reference to examples, but the scope of the present invention is not limited to these.

[0015]

[Embodiment 1] In this embodiment, an example of a method for extracting a spikelet is shown. First, about 100 g of the dried product of Panicle millet was crushed with a mixer, the crushed product and 500 ml of 50% ethyl alcohol were placed in a flask, and reflux extraction was carried out at 50 ° C. for 1 hour while stirring.

After the extraction, this solution was suction filtered, the obtained filtrate was concentrated under reduced pressure at 50 ° C. using an evaporator, and then the obtained concentrated liquid was dried under reduced pressure to obtain 7.2 g of a brown powder.

In the same manner as described above, dried products of amor, buffalo uri, achote and guacol were prepared in 10 parts each.
14.4 each as an extract from crushed 0 g
g, 15.3 g, 7.2 g and 8.4 g were obtained.

[0018]

Example 2 In this example, the amor obtained in Example 1
The melanin production inhibitory effect of each extract of Panicle, Buffalo Ume, Achote and Guacol was measured.
First, B16 melanoma cells (B16FO, ATCC) that are melanin-producing malignant melanoma cells derived from mice.
No. CRL-6322) with fetal bovine serum at a final concentration of 1
The cells were cultured in Eagle's MEM medium added to 0%, and 6 ml of the above medium containing the cells at a concentration of 3 × 10 ³ cells / ml was placed in each well of a 6-well plate (FALCON), and CO ₂ was added. Incubator (5% CO ₂ , 3,
The cells were cultured at 7 ° C for 5 days.

Next, this medium was replaced with a new Eagle MEM medium (6 ml) containing 0.03% theophylline (manufactured by SIGMA), and an appropriate amount of the sample solution (extract obtained in Example 1 was added to each well. After the addition of the aqueous solution), the cells were further cultured for 3 days. After completion of the culture, the medium was discarded, 1 ml of physiological saline was added to each well, and the cells attached to the bottom of the well were suspended by scraping with a scraper.

Next, the cell suspension was transferred to a microcentrifuge tube (1.5 ml volume, manufactured by Eppendorf) using a pipette and centrifuged (1,000 × g, 15 minutes).
did.

On the other hand, as a control, sterilized water was added in place of the sample solution, and the same test as above was conducted. In addition, as a test group for comparing the whitening of cells, 2% was used instead of the sample solution.
L-ascorbic acid aqueous solution (a) 60 μl, (b) 1
50 μl and 300 μl of (c) were added, and the same test as above was conducted.

Next, the degree of whiteness of the pelletized cells was visually compared to determine the effect of suppressing melanin production. In this case, the degree of whiteness of cells in the control experiment group (sterile water addition group) was "-", and the degree of whiteness of cells in the comparative experiment group to which L-ascorbic acid was added was (a):
“+”, (B): “++”, (c): “++++”, the degree of whiteness of the cells when the sample solution was added,
It was visually judged which of-, +, ++ and +++ it corresponds to, and the strength of the melanin production inhibitory effect of the sample solution was judged in four stages, and the results are shown in Table 1.

[0023]

[Table 1]

As is clear from the results shown in Table 1, each of the extracts of Panicle, Buffalo Ume, Achote and Guayule at a concentration of 200 μg / ml contained 5 L-ascorbic acid.
It exhibited a melanin production inhibitory effect equivalent to that of 00 μg / ml.

Further, it was confirmed that the extract of amor exhibits a melanin production inhibitory effect equivalent to 1000 μg / ml of L-ascorbic acid at a low concentration of 50 μg / ml, and inhibits melanin production at a concentration lower than that of L-ascorbic acid. Was done.

[0026]

[Embodiment 3] In this embodiment, an example of blending the extract of Panicle Amaranthus obtained in Example 1 into a whitening cosmetic composition is shown.

(% By weight) Ear spikelet 1.0 Glycerin 5.0 Polyoxyethylene sorbitan monolaurate 1.5 Ethanol 10.0 Fragrance appropriate amount Preservative, antioxidant appropriate amount pigment appropriate amount purified water balance

[0028]

EFFECTS OF THE INVENTION As described above, the extracts of Central American plants such as amor, panicle, buffalouri, achote and guayule have an action of suppressing melanin production in melanoma cells, and among these extracts The whitening cosmetic composition of the present invention containing at least one kind has an excellent skin whitening effect. In addition, these extracts exhibit an excellent skin whitening effect even in a small amount and have low toxicity to cells, and thus are safe. It is expensive.

Claims (1)

[Claims] 1. Amor, spikelet, buffalo,
A whitening cosmetic comprising an extract obtained from at least one plant selected from the group consisting of achote and guayule.