JP3231908B2 - Whitening cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a whitening cosmetic having a skin whitening effect, and more particularly to a whitening cosmetic in which a crude drug extract having a whitening effect based on a melanin production inhibitory action is blended as an active ingredient.

[0002]

2. Description of the Related Art In general, it is considered that darkening, spots, freckles, and the like due to sunburn are caused by the production of melanin, which is a black-brown amorphous pigment. This melanin causes external stimuli such as ultraviolet rays on the skin. When it is received, tyrosinase (tyrosine oxidase) present in the melanocytes of the skin is activated, and tyrosine, one of the amino acids constituting the protein, is oxidized and produced.

[0003] Accordingly, since an effect of whitening skin is expected by suppressing the activity of tyrosinase involved in melanin production, it has been proposed to add a tyrosinase activity-inhibiting component to cosmetics.

Conventionally, as a whitening cosmetic having a whitening effect, as disclosed in JP-B-55-43443, "Whitening cosmetic" and JP-B-54-974, "Composition of crude drug extract", ascorbin Known are those containing an acid or a derivative thereof, an external preparation for skin containing arbutin (JP-A-60-16906, etc.), a bleaching cosmetic containing kojic acid (Japanese Patent Publication No. 32-8100), Cosmetics extracted from plant components (JP-A-63-2913 and others) or animal components (JP-A-63-8312 and others) are known to have a whitening effect.

However, many of the above-mentioned conventional cosmetics do not have a sufficient whitening effect, and also have problems in safety to the skin such as insufficient storage stability and irritation. There were many.

[0006]

DISCLOSURE OF THE INVENTION The present invention solves the above-mentioned problems of the prior art, and has a novel skin whitening effect having an excellent skin whitening effect, sufficient storage stability and high safety. The purpose is to provide.

[0007]

Means for Solving the Problems The present inventors have conducted intensive studies in order to solve such problems, and found that crude drug extracts of Soju and anti-Chrysanthemum have an inhibitory effect on melanin production in melanoma cells, The present invention could be provided.

[0008] That is, the present invention relates to a whitening cosmetic comprising an extract of at least one crude drug selected from the group consisting of Soju and anti-Chrysanthemum.

[0009]

The cosmetic of the present invention can be manufactured by the following method. First, pulverized products of Sojuju and anti-Chrysanthemum flower are extracted by heating using an extraction solvent.

In this case, as the extraction solvent, alcohols such as methanol, ethanol, propanol or isopropyl alcohol, water and the like can be used alone or as a mixed solution thereof. Extraction at 50 ° C. for 1 hour using 50% aqueous alcohol improves the extraction efficiency.

After extraction, the extract is filtered to obtain an extract, and the extract is concentrated under reduced pressure while heating at a temperature of 60 ° C. or lower to dryness. And blend it into cosmetics. In this case, the same effect can be obtained even if the above-mentioned extract is directly added to the cosmetic.

[0012] The inventors of the present invention have found that the crude drug extracts of sojujutsu and anti-chrysanthemum flowers thus obtained exhibit excellent melanin production inhibitory action at a low concentration as compared with conventionally used ascorbic acid. Has been confirmed by testing,
By blending this extract as an active ingredient in an amount of 0.01 to 5.0%, a whitening cosmetic having a whitening effect can be obtained.

Hereinafter, the present invention will be described in more detail by way of examples, but the scope of the present invention is not limited thereto.

[0014]

Embodiment 1 In this embodiment, an example of a method for extracting crude drugs will be described. First, about 100 g of crude drug, Soju, was pulverized with a mixer, and the pulverized product and 500 ml of 50% ethyl alcohol were placed in a flask and refluxed at 50 ° C. for 1 hour with stirring.

After the extraction, this solution was subjected to suction filtration, and the obtained filtrate was concentrated under reduced pressure at 50 ° C. using an evaporator.
Next, the concentrated liquid was dried under reduced pressure to obtain 17.9 g of brown crystals.

In the same manner as above, 13.8 g of an extract was obtained from about 100 g of anti-chrysanthemum as a crude drug.

[0017]

Example 2 In this example, the melanin production inhibitory effect of each extract obtained in Example 1 was measured. First, B16, a malignant melanoma cell derived from a mouse that produces melanin,
Melanoma cells (B16Fo, ATCC No. CRL)
-6322) was cultured in Eagle's MEM medium supplemented with fetal bovine serum to a final concentration of 10%, and the cells were added to each well of a 6-well plate (manufactured by FALCON).
Add 6 ml of the above medium containing a concentration of × 10 ³ cell / ml,
The cells were cultured in an O ₂ incubator (5% CO ₂ , 37 ° C.) for 5 days.

Next, this medium was replaced with a new Eagle MEM medium (6 ml) containing 0.03% theophylline (manufactured by SIGMA), and an appropriate amount of the sample solution (the extract obtained in Example 1) was added to each well. Was further cultured for 3 days. After completion of the culture, the medium was discarded from the culture solution, 1 ml of physiological saline was added to each well, and the cells adhered to the bottom of the well were suspended using a scraper, and then suspended using a pipette. The cell suspension was transferred to a microcentrifuge tube (1.5 ml volume, manufactured by Eppendorf) and centrifuged (1,000 × g, 15 minutes).
did.

On the other hand, as a control, the same test as above was conducted by adding sterile water instead of the sample solution. In addition, as an experimental group for comparing the whitening of the cells, instead of the sample solution, 2
% (L) -ascorbic acid aqueous solution (a) 60 μl, (b)
150 μl and (c) 300 μl were added, and the same test was performed.

Next, the whiteness of the pelleted cells was visually compared to determine the melanin production inhibitory action.
In this case, the degree of whiteness of the cells in the control group (sterilized water-added group) was "-", and the degree of whiteness of the cells in the experimental group to which L-ascorbic acid was added was (a): "+", (b) ):
"++", (c): As "++++", the degree of whiteness of the cells when the sample solution was added was visually judged to determine which of these, and the strength of the melanin production inhibitory effect of the sample solution. , And the results are shown in Table 1.

[0021]

[Table 1]

[0022] As can be seen from the results shown in Table 1, the extracts of Sophora autumatum and Anti-Chrysanthemum flower show a melanin production inhibitory action at a concentration of 200 µg / ml equivalent to 500 µg / ml of L-ascorbic acid. It was confirmed that melanin production was suppressed at a much lower concentration. In addition, even at a high concentration of 800 μg / ml, there was no toxicity to cells, and safety was confirmed.

[0023]

Example 3 In this example, an example of blending the extract of Sophora jujuta obtained in Example 1 with a whitening cosmetic is shown.

(% By weight) Soju extract 1.0 Glycerin 5.0 Polyoxyethylene sorbitan monolaurate 1.5 Ethanol 10.0 Appropriate perfume Preservative, antioxidant Appropriate dye Appropriate purified water Remainder

[0025]

EFFECTS OF THE INVENTION As described above, the extracts of C. aureus and anti-chrysanthemum used in the present invention exhibit an excellent melanin production inhibitory effect on melanoma cells. Therefore, one or more of these extracts may be used. The formulated whitening cosmetics exhibit an excellent skin whitening effect, and these extracts can provide highly safe products because of their low toxicity to cells.

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Claims (1)

(57) [Claims] (1) Sojutsu and anti-chrysanthemum (Kou)
A whitening cosmetic comprising an extract of at least one crude drug selected from the group consisting of Kicca) .