JP2904628B2 - Fair skin - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a whitening cosmetic having a skin whitening effect, and more particularly to a whitening cosmetic in which a plant extract having a whitening effect based on a melanin production inhibitory action is blended as an active ingredient.

[0002]

2. Description of the Related Art It is considered that darkening, spots, freckles, and the like due to sunburn are caused by the production of melanin, a black-brown amorphous pigment, and this melanin is exposed to external stimuli such as ultraviolet rays. Tyrosinase (tyrosine oxidase) present in melanocytes of the skin is activated, and tyrosine, one of the amino acids constituting protein, is oxidized and produced. Therefore, since an effect of whitening skin is expected by suppressing the activity of tyrosinase involved in melanin production, it has been proposed to add a tyrosinase activity inhibitory component to cosmetics.

[0003] Conventionally, as whitening cosmetics having a whitening effect, as disclosed in JP-B-55-43443, "Whitening cosmetics" and JP-B-54-974, "Composition of crude drug extract", ascorbic acid or What blended the derivative is known. In addition, skin external preparations containing arbutin (JP-A-60-16906), bleaching cosmetics containing kojic acid (Japanese Patent Publication No. 32-8100), plant components (JP-A-63-2913, etc.) ) Or animal components (JP-A-63-8312, etc.) are known as having a whitening effect.

[0004] However, many of the above-mentioned conventional cosmetics do not show a sufficient whitening effect, and some of them have problems in safety to the skin such as insufficient storage stability and irritation. There were many.

[0005]

SUMMARY OF THE INVENTION The present invention solves the above-mentioned problems of the prior art and provides a fair-skin cosmetic having an excellent skin whitening effect, sufficient storage stability and high safety. The purpose is to do.

[0006]

Means for Solving the Problems The present inventors have made intensive studies to solve the above problems, and as a result, the extract of Floro de Manita showed an inhibitory effect on tyrosinase enzyme activity and melanoma cells. The present invention was found to have a melanin production inhibitory effect, and could provide the present invention.

That is, the present invention provides a fair-skin cosmetic characterized by containing an extract of Floro de Manita, a plant native to Mexico.

[0008]

The cosmetic of the present invention can be manufactured by the following method. First, Flor de Manita, a kind of plant native to Mexico [also known as Flor de Manita]
MACPALXOCHITL], or a dry ground product is extracted by heating using an extraction solvent. In addition, as the extraction solvent, alcohol (methanol, ethanol, propanol, isopropyl alcohol, or the like), water, or the like can be used, and a mixed solution thereof can also be used. For example, extraction efficiency is good when extraction is performed at 50 ° C. for 1 hour using hydroalcohol having an alcohol concentration of 20 to 70%.

After extraction, the extract is filtered to obtain an extract. The obtained extract is further concentrated under reduced pressure while heating at a temperature of 60 ° C. or lower to dryness, and the dried extract is collected and blended into cosmetics. In addition, you may mix the said extract with cosmetics as it is.

[0010] The present inventors have found that the extract of Floro de Manita thus obtained exhibits an excellent inhibitory action on melanin production at a low concentration as compared with conventionally used ascorbic acid. By adding this extract as an active ingredient in an amount of 0.01 to 5.0%, a whitening cosmetic having a whitening effect can be obtained.

Hereinafter, the present invention will be described in more detail with reference to examples. However, the scope of the present invention is not limited by the following examples.

[0012]

Embodiment 1 In this embodiment, an example of a method for extracting Floro de Manita will be described. First, one of the plants native to Mexico
Flor de Manita is a seed
About 100 g of the dried product of [also called MACPALXOCHITL] was pulverized with a mixer, and the pulverized product and 1 liter of 50% ethyl alcohol were placed in a flask and refluxed at 50 ° C. for 1 hour with stirring. After the extraction, this solution was subjected to suction filtration, and the obtained filtrate was concentrated under reduced pressure at 50 ° C. using an evaporator. Then, the obtained concentrated liquid was dried under reduced pressure, and 10 g
A brown crystal (extract) was obtained.

[0013]

[Embodiment 2] In this embodiment, the flowmeter obtained in Embodiment 1 is used.
The tyrosinase activity inhibitory effect of the extract of De Manita was measured. The tyrosinase activity inhibitory effect was measured by a method of quantifying dopachrome produced from dopa by tyrosinase by measuring absorbance at 475 nm. In measuring the tyrosinase activity inhibitory action, the following reaction reagents were used. (A) Sodium succinate buffer (100 mM, pH 5.
5) (b) Mushroom tyrosinase (SIGMA) solution (adjusted to 270 units / ml with (a) buffer) (c) L-DOPA (Wako Pure Chemical Industries) solution (6 mM to (b) buffer) Adjustment)

First, 1.8 ml of the buffer (a) was placed in a test tube.
0.1 ml of the tyrosinase solution of (b) and (2) were added to the test tubes, respectively, at 2% (w / v), 1% (w / v) or 0.5% (w).
(v) 0.1 ml of a sample solution (an aqueous solution of the extract obtained in Example 1) of each concentration was added, and the mixture was incubated for 15 minutes in a thermostatic water layer at 30 ° C. Next, the L-DOPA solution of (c) was added to this test tube.
After adding 1 ml and stirring, the test tube was set at about 45 ° in a reciprocating shaker in a thermostatic chamber at 30 ° C., and shaken for 40 minutes (reciprocation frequency 150 / min). After shaking, use a spectrophotometer for 4
The absorbance at 75 nm was measured, and the measured value was designated as A.

On the other hand, the absorbance at 475 nm was measured in the same manner as above except that the buffer (a) was added instead of the sample solution as a control.
Further, the absorbance at 475 nm was measured in the same manner as above except that the buffer (a) was added instead of the L-DOPA solution as a blank.

The tyrosinase activity inhibition rate of the sample solution was calculated from the measured value of the absorbance at 475 nm. The calculation of the tyrosinase activity inhibition rate is performed using the following formula,
The results are shown in Table 1. Tyrosinase activity inhibition rate (%) = ｛1- (AC) /
B｝ × 100

[Table 1]

As can be seen from Table 1, Floro de.
Manita extract, even at a low concentration of 0.5% aqueous solution, inhibits tyrosinase activity by as much as 59%.
It was confirmed to have an extremely excellent tyrosinase activity inhibitory action.

[0018]

[Embodiment 3] In this embodiment, the flow meter obtained in Embodiment 1 is used.
The melanin production inhibitory effect of the de Manita extract was measured.

First, B16 melanoma cells (B16-F0, ATCC), which are malignant melanoma cells derived from mice that produce melanin
No. CRL-6322) was cultured in Eagle's MEM medium supplemented with fetal bovine serum to a final concentration of 10%, and the cells were added to each well of a 6-well plate (FALCON) at 3 × 10 5.
⁶ ml of the above medium containing ³ cells / ml was added and cultured in a CO ₂ incubator (5% CO ₂ , 37 ° C.) for 5 days.

Next, the medium was added with 0.03% theophylline.
New Eagle MEM medium (6 ml) containing (SIGMA)
After adding an appropriate amount of a sample solution (aqueous solution of the extract obtained in Example 1) to each well, the cells were further cultured for 3 days. After completion of the culture, the medium was discarded from the culture solution, 1 ml of physiological saline was added to each well, and the cells adhered to the bottom of the well were suspended using a scraper to scrape the cells. Next, the cell suspension was transferred to a microcentrifuge tube (1.5 ml volume, manufactured by Eppendorf) using a pipette.
Centrifugation (1,000 xg, 15 minutes).

On the other hand, as a control, the same test as described above was conducted by adding sterilized water instead of the sample solution. In addition, as an experimental group for comparing the whitening of the cells, a 2% L-ascorbic acid aqueous solution was used instead of the sample solution (a) 60 μl, (b) 150 μl.
μl and (c) 300 μl were added, and the same test was performed.

Next, the degree of whitening of the pelleted cells was visually compared to determine the melanin production inhibitory effect. At that time, the degree of whiteness of the cells in the control experiment (sterilized water-added) was "-", and the degree of whiteness of the cells in the comparative experiment to which L-ascorbic acid was added was (a): "+", ( b)
: “++”, (c): As “++++”, the degree of whiteness of the cells when the sample solution was added was −, +, ++, +++
It was visually determined which one of the two corresponded, and the strength of the melanin production inhibitory effect of the sample solution was determined in four steps. The results are shown in Table 2.

[Table 2]

As can be seen from Table 2, Floro de.
At a concentration of 200 μg / ml, the extract of Manita showed an inhibitory effect on melanin production equivalent to 500 μg / ml of L-ascorbic acid,
It was confirmed that melanin production was suppressed at a much lower concentration than L-ascorbic acid. Even at a high concentration of 1,000 μg / ml, there was no toxicity to cells.

[0024]

[Embodiment 4] In this embodiment, the flowmeter obtained in Embodiment 1 is used.
An example of blending the extract of De Manita into a fair skin cosmetic is shown. (Wt%) Floro de Manita extract 1.0 Glycerin 5.0 Polyoxyethylene sorbitan monolaurate 1.5 Ethanol 10.0 Perfume Appropriate Preservative, antioxidant Appropriate pigment Appropriate amount Purified water Remainder

[0025]

The extract of Floro de Manita, a plant native to Mexico, has an excellent inhibitory effect on tyrosinase activity, and a fair skin cosmetic containing this extract exhibits an excellent skin whitening effect. I do. In addition, the extract of Floro de Manita exhibits excellent skin whitening effect in a small amount and has low toxicity to cells, so that it is highly safe.

────────────────────────────────────────────────── ─── Continued on the front page (58) Fields surveyed (Int. Cl. ⁶ , DB name) A61K 7/00-7/50 A61K 35/78 WPI (DIALOG)

Claims (1)

(57) [Claims] 1. A fair-skin cosmetic containing an extract of Flor de Manita, a plant native to Mexico.