JPH06219934A - Skin-whitening cosmetic - Google Patents

Abstract

PURPOSE:To obtain a skin-whitening cosmetic having excellent skin whitening effect and sufficient storage stability and high safety. CONSTITUTION:The skin-whitening cosmetic is obtained by blending at least one extract of galenical drug selected from the group consisting of Pinellie Tuber, Trichosanthis Fructus, Trichosanthis Radix and Dioscoreae radix. Concretely, about 100g of dried material of Pinellie Tuber which is a galenical drug is pulverized by a mixer and pulverized material thereof and 500ml 50% ethyl alcohol are put in flask and the pulverized material is subjected to reflux extraction at 50 deg.C for 1hr while stirring. After extraction, this solution is filtered with suction and the resultant filtrate is concentrated under reduced pressure at 50 deg.C using an evaporator. Then the resultant concentrate is dried under reduced pressure to provide 3.6g brawn crystal. 14.5g, 6.2 g and 3.2g extracts are obtained from about 100g dried material of Trichosanthis Fructus, trichosanthis Radix and Dioscoreae radix which are galenical drugs as similarly as mentioned above, respectively. The resultant extract is blended as an active ingredient in amount of 0.01-5.0% based on the cosmetic.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a whitening cosmetic composition having a skin whitening effect, and more particularly to a whitening cosmetic composition containing, as an active ingredient, a crude drug extract having a whitening effect based on a melanin production inhibitory effect.

[0002]

2. Description of the Related Art Generally, it is believed that dark skin, spots, freckles, etc. due to sunburn are caused by the production of melanin, which is a dark brown amorphous pigment, and this melanin causes the skin to receive external stimuli such as ultraviolet rays. Upon receiving it, tyrosinase (tyrosine oxidase) present in the melanocytes of the skin is activated, and tyrosine, which is one of the protein-constituting amino acids, is oxidized and produced. For this reason, it has been proposed to incorporate a component having a melanin production suppressing action such as inactivation of melanocytes and suppression or inhibition of tyrosinase activity into cosmetics.

Conventionally, as a whitening cosmetic having a whitening effect, ascorbic acid or a derivative thereof, as disclosed in JP-B-55-43443 "Whitening cosmetic" and JP-B-54-974 "Crude drug extract-containing composition". It is known that a mixture of In addition, a skin external preparation containing arbutin and a bleaching cosmetic containing kojic acid (Japanese Patent Publication No. 32-8).
100), a plant component (JP-A-63-2913, etc.) or an animal component (JP-A-63-3812) is known to have a whitening effect.

However, many of the above-mentioned conventional cosmetics do not have a sufficient whitening effect, and also have some problems such as insufficient storage stability and irritation, which are problematic to the skin. There were many.

[0005]

The object of the present invention is to provide a whitening cosmetic composition which solves the above-mentioned conventional problems, has an excellent whitening effect, and has sufficient storage stability and high safety. And

[0006]

Means for Solving the Problems As a result of intensive studies for solving the above problems, the present invention found that a crude drug extract of Hange, Karojitsu, Carocon and Sanyaku has an action of suppressing melanin production in melanoma cells. The invention could be provided.

[0007] That is, the present invention provides a whitening cosmetic composition containing an extract of at least one crude drug selected from the group consisting of Hanghe, Karoditsu, Carocon and Sanyaku.

[0008]

The cosmetic of the present invention can be produced by the following method. First, a dried product or a dried product crushed product of Hange, Karoditsu, Carocon and Sanyaku is heated and extracted using an extraction solvent. As the extraction solvent, alcohol (methanol, ethanol, propanol, isopropyl alcohol, or the like), water, or the like can be used, or a mixed solvent thereof can also be used.
For example, extraction efficiency is good if hydrous alcohol having an alcohol concentration of 20 to 70% is used and extraction is performed at 50 ° C. for 1 hour.

After the extraction, the extract is filtered to obtain an extract. The obtained extract is further concentrated under reduced pressure while heating at a temperature of 60 ° C. or lower to dryness, and the dry extract is collected and blended in a cosmetic. In addition, you may mix | blend the said extract with cosmetics as it is.

The herbal medicine extracts of Hange, Karoditsu, Carocon and Sanyaku obtained in this manner are expected to exert an excellent melanin production inhibitory effect at a low concentration, as compared with conventionally used ascorbic acid. It has been confirmed by the inventors that this extract is 0.01% as an active ingredient.
By blending up to 5.0%, a whitening cosmetic having a whitening effect can be obtained.

Hereinafter, the present invention will be described in more detail with reference to examples. However, the scope of the present invention is not limited by the following examples.

[0012]

Example 1 In this example, an example of a method for extracting a crude drug will be described. First, about 100 g of the dried product of Hange, which is a crude drug, was crushed with a mixer, and the crushed product and 500 ml of 50% ethyl alcohol were placed in a flask and stirred at 50 ° C.
Reflux extraction was performed for 1 hour. After extraction, this solution was suction filtered, and the obtained filtrate was concentrated under reduced pressure at 50 ° C. using an evaporator. Then, the obtained concentrated liquid is dried under reduced pressure,
3.6 g of brown crystals were obtained.

[0013] Further, as in the above, the herbal medicine Carodizu,
Approximately 100 g of calocon and sanyaku dried products yielded 14.5 g, 6.2 g and 3.2 g of extract, respectively.

[0014]

Example 2 In this example, the melanin production inhibitory action of each of the herbal medicine extracts of Hange, Karoditsu, Carocon and Sanyaku obtained in Example 1 was measured. First, B16 melanoma cells (B16-FO, ATCC No. CRL-63), which are melanin-producing mouse-derived malignant melanoma cells.
22) was cultured in Eagle MEN medium supplemented with fetal bovine serum to a final concentration of 10%, and 3 × 10 6 of the cells was placed in each well of a 6-well plate (FALCON).
Add 6 ml of the above medium containing ³ Cell / ml concentration,
5 in a CO ₂ incubator (5% CO ₂ , 37 ° C)
Cultured for a day.

Then, this medium was replaced with a new Eagle MEM medium (6 ml) containing 0.03% theophylline (manufactured by SIGMA), and an appropriate amount of the sample solution (extract obtained in Example 1 was added to each well. After the addition of the aqueous solution), the cells were further cultured for 3 days. After the completion of the culture, the medium was discarded, 1 ml of physiological saline was added to each well, and the cells attached to the bottom of the well were suspended using a scraper so as to be scraped. Next, the cell suspension was pipetted into a microcentrifuge tube (1.5 ml volume, manufactured by Eppendorf).
And was centrifuged (1000 × g, 15 minutes).

On the other hand, as a control, sterilized water was added instead of the sample solution and the same test as above was conducted. In addition, as an experimental section for comparing the whiteness of cells, 60 μl of (a) 2% L-ascorbic acid aqueous solution was used instead of the sample solution.
(B) 150 μl and (c) 300 μl were added, and the same test as above was conducted.

Next, the whiteness of the pelletized cells was visually compared to determine the melanin production inhibitory effect. At that time, the degree of whiteness of cells in the control experimental section (sterile water-added section) was “−”, and the degree of whiteness of cells in the experimental section to which L-ascorbic acid was added was (a): “+”, (b ):
“++”, (c): As “++++”, the degree of whiteness of the cells when the sample solution was added was −, +, ++, ++++.
It was judged visually which of the above corresponds to the strength of the melanin production inhibitory effect of the sample solution, and the judgment was carried out in four steps.
The results are shown in Table 1.

[0018]

[Table 1]

As can be seen from Table 1, each extract of Hange, Karojitsu, Carocon and Sanyaku is 200 μg / m 2.
At a concentration of 1 L, as much as 500 μg / ml of L-ascorbic acid showed a melanin production inhibitory action. In addition, the extract of carocon was L-ascorbic acid 1 at a low concentration of 50 μg / ml.
It was confirmed that it showed a melanin production inhibitory effect equivalent to 000 μg / ml and suppressed melanin production at a lower concentration than L-ascorbic acid. Furthermore, each extract is 800 μg
There was no toxicity to cells even at the high concentration of / ml.

[0020]

Example 3 In this example, an example of blending the extract of the herbal medicine Hange obtained in Example 1 into a whitening cosmetic composition is shown. (Wt%) Hange extract 1.0 Glycerin 5.0 Polyoxyethylene sorbitan monolaurate 1.5 Ethanol 10.0 Fragrance Suitable amount Preservative, antioxidants Suitable amount Pigment Suitable amount Purified water Remainder

[0021]

EFFECTS OF THE INVENTION Since the extracts of Hangge, Caroditu, Carocon and Sanyaku exert excellent melanin production inhibitory action on melanoma cells, whitening cosmetics containing one or more of these extracts are excellent. Exhibits the skin whitening effect. In addition, the extracts of Hange, Karojitsu, Carocon and Sanyaku are highly safe because they have low toxicity to cells.

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[Procedure amendment]

[Submission date] January 17, 1994

[Procedure 3]

[Document name to be amended] Statement

[Name of item to be corrected] 0003

[Correction method] Change

[Correction content]

Conventionally, as a whitening cosmetic composition having a whitening effect, as disclosed in Japanese Patent Publication No. 55-43443 "whitening cosmetic" and JP-B-54-974 "herbal extract blend composition", ascorbic acid or a Those containing a derivative are known. In addition, a skin external preparation containing arbutin and a bleaching cosmetic containing kojic acid (Japanese Patent Publication No.
8100), plant components (JP-A-63-2913, etc.)
Alternatively, cosmetics extracted from animal components (Japanese Patent Laid-Open No. 63-3812) are known to have a whitening effect.

[Procedure amendment 4]

[Document name to be amended] Statement

[Correction target item name] 0014

[Correction method] Change

[Correction content]

[0014]

Example 2 In this example, the hanger obtained in Example 1
The melanin production inhibitory action of each crude drug extract of Caroditu, Carocon and Sanyaku was measured. First, B16 melanoma cells (B16-FO, ATCC No. CRL-6), which are melanin-producing mouse-derived malignant melanoma cells.
322), were cultured in the added Eagle ME M medium to the fetal calf serum to a final concentration of 10% to each well of a 6 well plate (FALCON Corp.), the cells 3 × 1
6 ml of the above medium containing a concentration of 0 ³ cell / ml was added and cultured in a CO ₂ incubator (5% CO ₂ , 37 ° C.) for 5 days.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Chen Jingban, No. 116, 3rd dan, Chongqing South Road, Taipei City, Taiwan (72) Inventor Masao Takahashi 4-11 Nihonbashi Kobunecho, Chuo-ku, Tokyo Junsei Industrial Co., Ltd. In the company

Claims (1)

[Claims] 1. A whitening cosmetic characterized in that it contains an extract of at least one herbal medicine selected from the group consisting of Hange, Karojitsu, Carocon and Sanyaku.