JP3231907B2 - Whitening cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic having a skin whitening effect, and more particularly, to a whitening cosmetic containing, as an active ingredient, a plant extract having a whitening effect based on an inhibitory action on melanin production.

[0002]

2. Description of the Related Art In general, it is considered that darkening, spots, freckles, and the like due to sunburn are caused by the production of melanin, which is a black-brown amorphous pigment. This melanin causes external stimuli such as ultraviolet rays on the skin. When it is received, tyrosinase (tyrosine oxidase) present in the melanocytes of the skin is activated, and tyrosine, one of the amino acids constituting the protein, is oxidized and produced.

[0003] Accordingly, since an effect of whitening skin is expected by suppressing the activity of tyrosinase involved in melanin production, it has been proposed to add a tyrosinase activity-inhibiting component to cosmetics.

Conventionally, as a whitening cosmetic having a whitening effect, as disclosed in Japanese Patent Publication No. 55-43443, "Whitening Cosmetic" and Japanese Patent Publication No. 54-974, "Composition of crude drug extract", ascorbic acid In addition to those containing a derivative thereof, an external preparation for skin containing arbutin (JP-A-60-16906), a bleaching cosmetic containing kojic acid (Japanese Patent Publication No. 32-8100), and a plant Cosmetics extracted from ingredients (JP-A-63-2913 and others) or animal ingredients (JP-A-63-8312 and others) are known to have a whitening effect.

However, many of the above-mentioned conventional cosmetics do not have a sufficient whitening effect, and also have problems in safety to the skin such as insufficient storage stability and irritation. There were many.

[0006]

DISCLOSURE OF THE INVENTION The present invention solves the above-mentioned problems of the prior art, and has a novel skin whitening effect having an excellent skin whitening effect, sufficient storage stability and high safety. The purpose is to provide.

[0007]

Means for Solving the Problems The present inventors have conducted intensive studies in order to solve the above problems, and as a result, the extracts of Mexican plants Thira, Camote de azafuran, Hamaica, Poleoverde and Nabonegro were found to contain tyrosinase enzyme. The present invention was found to have an activity inhibitory effect and an inhibitory effect on melanin production in melanoma cells, and could provide the present invention.

[0008] That is, the present invention relates to a tila (TILA),
CAMOTE DE AZAF
RAN), Jamaica, POLEO VERDE and Navonegro (NAV)
At least one selected from the group consisting of O NEGRO)
The present invention relates to a whitening cosmetic comprising an extract obtained from a seed plant.

[0009]

The cosmetic of the present invention can be manufactured by the following method. First, at least one of a Mexican plant, Thira, Camote de azafuran, Hamaica, Poleoverde, and Nabonegro, is dried and heat-extracted using an extraction solvent.

In this case, as the extraction solvent, alcohols such as methanol, ethanol, propanol or isopropyl alcohol, water, or a mixed solution thereof can be used.
It has been confirmed that an extraction efficiency of 1 hour at 50 ° C. using 0-70% aqueous alcohol is good.

After the extraction, the extract is filtered to obtain an extract, and the extract is further concentrated under reduced pressure while heating at a temperature of 60 ° C. or less to dryness. In this case, the above-mentioned extract may be directly blended with the cosmetic.

The extract of the plant (tila, camote de azafuran, Hamaica, oleoverde, nabonegro) obtained in this way has a lower concentration of melanin production than the conventionally used ascorbic acid. It has been confirmed by the present inventors that it exerts an action, and a whitening cosmetic having a whitening effect can be obtained by incorporating this extract as an active ingredient in an amount of 0.01 to 5.0%.

[0013] As the dried plant which can be used in the present invention, each part of the plant such as flowers, leaves, stems and roots can be used.

Hereinafter, the present invention will be described in detail with reference to Examples, but the scope of the present invention is not limited thereto.

[0015]

Embodiment 1 In this embodiment, an example of a method for extracting a plant will be described. First, about 100 g of a dried product consisting of the flower of Thira, a kind of Mexican plant, is pulverized with a mixer, and then the pulverized product and 1 liter of 50% ethyl alcohol are placed in a flask and stirred at 50 ° C. for 1 hour. Reflux extraction was performed.

After the extraction, this solution was subjected to suction filtration, and the obtained filtrate was concentrated under reduced pressure at 50 ° C. using an evaporator. Then, the obtained concentrated liquid was dried under reduced pressure to obtain 10.5 g of brown crystals (extracted product). ) Got.

In the same manner as described above, 14.4 g, 15.3 g, and 14.3 g, respectively, are obtained by pulverizing about 100 g of a dried product of Camote de azafuran, Hamaica, Poleoverde and Nabonegro, which are Mexican plants.
7.2 g and 8.4 g were obtained.

[0018]

Example 2 In this example, the tyrosinase activity inhibitory effect of the extracts of Thira, Camote de azafuran, Hamaica, Poleoverde and Nabonegro obtained in Example 1 was measured.

In this case, in order to measure the tyrosinase activity inhibitory action, a method was used in which dopachrome produced from dopa by tyrosinase was quantified by measuring absorbance at 475 nm. In this method, the following reaction reagent was used. Using.

(A) Sodium succinate buffer
(100 mM, pH 5.5) (b) Mushroom tyrosinase (manufactured by SIGMA)
Solution (adjusted to 270 unit / ml with buffer (a)) (c) L-DOPA (manufactured by Wako Pure Chemical Industries) solution (adjusted to 6 mM with buffer (a))

First, 1.8 ml of the buffer (a) and the tyrosinase solution (b) were placed in a predetermined number of test tubes.
1 ml, then 2% (w / v), 1% (w / v), 0.5% consisting of an aqueous solution of the extract obtained in Example 1
(W / v), 0.1 ml of each sample solution of
The mixture was incubated for 15 minutes in a constant temperature water bath at 0 ° C.

Next, the L-DOP of (c) was added to this test tube.
After adding 1 ml of the solution A and stirring, the test tube was set at about 45 ° in a reciprocating shaker in a constant temperature room at 30 ° C.
It was shaken for 150 minutes (the number of reciprocations was 150 / min). After shaking, the absorbance at 475 nm was measured using a spectrophotometer.

On the other hand, the value obtained by measuring the absorbance at 475 nm in the same manner as described above except that the buffer (a) was added instead of the sample solution as a control was B, and a blank was used instead of the L-DOPA solution ( The absorbance at 475 nm was measured in the same manner as described above except that the buffer of b) was added, and the measured value was designated as C.

The tyrosinase activity inhibition rate was calculated as follows:
The calculation was performed using the following formula, and the results are shown in Table 1.

Tyrosinase activity inhibition rate (%) = ｛1-
(AC) / B｝ × 100

[0026]

[Table 1]

As is clear from Table 1, the extracts of Thira, Camote de azafuran, Hamaica, Boleoverde and Nabonegro strongly inhibit tyrosinase activity even at a low concentration of 2% aqueous solution. It was confirmed that the compound had an excellent tyrosinase activity inhibitory action.

[0028]

Example 3 In this example, the melanin production inhibitory effect of each extract obtained in Example 1 was measured. First, B16, a malignant melanoma cell derived from a mouse that produces melanin,
Melanoma cells (B16Fo, ATCC No. CRL)
-6322) was cultured in Eagle's MEM medium supplemented with fetal bovine serum to a final concentration of 10%, and the cells were added to each well of a 6-well plate (manufactured by FALCON).
Add 6 ml of the above medium containing a concentration of × 10 ³ cell / ml,
The cells were cultured in an O ² incubator (5% CO ₂ , 37 ° C.) for 5 days.

Next, this medium was replaced with a new Eagle MEM medium (6 ml) containing 0.03% theophylline (manufactured by SIGMA), and an appropriate amount of the sample solution (the extract obtained in Example 1) was added to each well. Was further cultured for 3 days. After completion of the culture, the medium was discarded from the culture, 1 ml of physiological saline was added to each well, and the cells adhered to the bottom of the well were suspended using a scraper to scrape the cells.

Next, the cell suspension was transferred to a microcentrifuge tube (1.5 ml volume, manufactured by Eppendorf) using a pipette, and centrifuged (1,000 × g, 15 minutes).
did.

On the other hand, as a control, the same test was performed by adding sterile water instead of the sample solution, and 2% L was used instead of the sample solution as an experimental group for comparing the whitening of cells.
-Ascorbic acid aqueous solution (a) 60 μl, (b) 15
0 μl and (c) 300 μl were added, and the same test was performed.

Next, the degree of whiteness of the pelleted cells was visually compared to determine the melanin production inhibitory effect. In this case, the degree of whiteness of the cells in the control group (sterilized water-added group) was "-", and the degree of whiteness of the cells in the comparative group to which L-ascorbic acid was added was (a):
“+”, (B): “++”, (c): “++”, the degree of whiteness of the cells when the sample solution was added,
Which of-, +, ++, and +++ the sample solution corresponds to was visually determined, and the strength of the melanin production inhibitory effect of the sample solution was determined in four stages, and the results are shown in Table 2.

[0033]

[Table 2]

As is clear from the results shown in Table 2, the tila extract was 200 μg / ml and ascorbic acid 500 μg / ml.
It showed melanin production inhibitory action equivalent to that of ml, and it was confirmed that melanin production was inhibited at a much lower concentration than L-ascorbic acid.

Also, Camote de Azafran, Hamaica,
Each of Poleoverde and Nabonegro extracts also showed the same melanin production inhibitory action as ascorbic acid. Furthermore, these extracts were not toxic to cells even at high concentrations of 1000 μg / ml.

[0036]

Example 4 In this example, an example of blending the extract of thira obtained in Example 1 with a whitening cosmetic is shown.

(Wt%) Tila extract 1.0 Glycerin 5.0 Polyoxyethylene sorbitan monolaurate 1.5 Ethanol 10.0 Appropriate perfume Preservative, antioxidant Appropriate dye Appropriate amount of purified water Remainder

[0038]

As described above, the extracts of Mexican plants Thira, Camote de azafuran, Hamaica, Poleoverde, and Nabonegro have excellent tyrosinase activity inhibitory activity and melanoma production inhibitory activity in melanoma cells. The whitening cosmetic of the present invention, which contains at least one of these extracts, has an excellent skin whitening effect. In addition, these extracts exhibit an excellent skin whitening effect in a small amount, and are effective for cells. Is highly safe because of its low toxicity.

──────────────────────────────────────────────────続 き Continuation of the front page (56) References FRAGANCE JOURNAL, extra edition, No. 6, (1986) FRAGANCE JOURNAL L, Vol. 18, No. 6, (1990) (58) Fields investigated (Int. Cl. ⁷ , DB name) A61K ^7/ 00-7/50 CA (STN)

Claims (1)

(57) [Claims] 1. Tila, CAMOTED AZAFRAN, JAMAICA, POLEO VE
A whitening cosmetic comprising an extract obtained from at least one plant selected from the group consisting of RDE) and Navonegro (NAVO NEGRO).