JP4520568B2 - Mushroom artificial culture medium and mushroom artificial cultivation method using the same - Google Patents
Mushroom artificial culture medium and mushroom artificial cultivation method using the same Download PDFInfo
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Description
【０００１】
【発明の属する技術分野】
本発明は、きのこの人工培養基及びそれを用いたきのこの人工栽培方法に関する。
なお、本発明でいう部や％は特に規定のないかぎり質量基準である。
【０００２】
【従来の技術とその課題】
従来、きのこの栽培は、くぬぎ、ぶな、及びなら等の原木を利用した、ほだ木栽培がほとんどであり、そのため、気象条件により収穫が左右されることが多いという課題があった。
【０００３】
また、最近では、ほだ木栽培用の原木切り出しのための労働力が不足していることなどによって原木の入手が困難になりつつあるという課題があった。
さらに、ほだ木栽培では栽培期間が長いこと、即ち、種菌の接種からきのこの収穫までに１年半から２年も要することにより、生産コストが相当高くつくという課題があった。
【０００４】
近年、えのきたけ、ひらたけ、なめこ、及びしいたけ等は、鋸屑に米糠を配合した人工培養基を用い、瓶又は箱で栽培を行う菌床人工栽培方法が確立され、一年を通して、四季に関係なく安定してきのこが収穫できるようになっている。
即ち、従来は農家での副業的性格が強く、小規模生産に頼っていたきのこ栽培が、現在では大規模専業生産が可能となり、かつ、原料が入手しやすい菌床人工栽培方法に移りつつある。
しかしながら、菌床人工栽培方法においても、きのこを大量に連続栽培するには、いまだ収率も低く、かつ、栽培期間がかなり長いため、その生産コストは安価とはいえず、今後これら生産性の改善が切望されている。
例えば、(Al2O3) X (SiO2)（ただし、式中のX は１以上の数）で示される化合物を前記の人工培養基に含有させたものや、(MgO) W (Al2O3) X (SiO2)y （ただし、式中のW は１〜３の数、X は１〜５の数、y は０〜３の数）で示される化合物を上記の人工培養基に含有させたもの、あるいはケイ酸アルミニウム、ケイ酸カルシウム、酸化第一鉄、酸化第二鉄、及び四三酸化鉄から選ばれるいずれか一種類の無機化合物を人工培養基に添加したものがあるが、充分な収率できのこを生産することができていないのが現状である（特開平03−210126号公報、特開平03−058716号公報、及び特開平07−322754号公報）。
【０００５】
本発明者は、きのこの人工栽培における従来の方法の課題を解決するため、誠意検討を重ねた結果、特定の人工培養基を使用することにより、きのこを高収率で栽培できることを見いだし、本発明を完成するに至った。
【０００６】
【課題を解決するための手段】
即ち、本発明は、ケイ酸カルシウム水和物を含有してなるきのこの人工培養基であって、前記ケイ酸カルシウム水和物の平均粒子径が10μｍ以下であり、前記ケイ酸カルシウム水和物の使用量が、前記人工培養基100部中、２〜10部であることを特徴とするきのこの人工培養基であり、さらに、硫酸塩を含有してなることを特徴とする該きのこの人工培養基であり、前記ケイ酸カルシウム水和物が、ゾノトライト、ＣＳＨ（II）及びトバモライトの中から選ばれた一種以上であることを特徴とする該きのこの人工培養基であり、これらの人工培養基を用いてなるきのこの人工栽培方法である。
【０００７】
【発明の実施の形態】
以下、本発明をさらに詳しく説明する。
【０００８】
本発明で使用するケイ酸カルシウム水和物とは、きのこの収率を向上するために必要なもので、ケイ石、ケイ砂、石英、及びケイ藻土等のシリカ原料と、生石灰(CaO)、消石灰(Ca(OH)2)、及び石灰石(CaCO3)等のカルシア原料を主原料として水熱合成して得られるものをいい、具体的には、ゾノトライト（6CaO・6SiO2・H2O）、ＣＳＨ（Ｉ）、ＣＳＨ（II）、トバモライト（5CaO・6SiO2・1〜9H2O）、ヒレブランダイト（2CaO・SiO2・H2O）、ジャイロライト、及びフォシャジャイト等の結晶性や、非結晶性の水和物が挙げられる。ケイ酸カルシウム水和物は、建材や断熱材用として大量に製造されており、これらの製造過程で発生するものや、施工現場における端材等を使用することも可能である。これらは、単独で使用してもよく、二種類以上を混合して使用することも可能である。ケイ酸カルシウム水和物の平均粒子径は、少量の添加量できのこの収率が向上することから小さいほど好ましい。具体的には、１mm以下が好ましく、100μｍ以下がより好ましく、10μｍ以下が最も好ましい。１mmを越えると、きのこの収率の向上が得られない場合がある。ケイ酸カルシウム水和物の使用量は、人工培養基100部中、0.01〜20部が好ましく、２〜10部がより好ましい。この範囲外ではきのこの収率の向上が得られない場合がある。
【０００９】
本発明において、ケイ酸カルシウム水和物に硫酸塩を併用することは、きのこの収率をさらに向上する面から好ましい。
硫酸塩としては、無水セッコウ、半水セッコウ、二水セッコウ、無水硫酸アルミニウム、含水硫酸アルミニウム、無水硫酸ナトリウム、含水硫酸ナトリウム、無水硫酸マグネシウム、含水硫酸マグネシウム、無水硫酸リチウム、及び含水硫酸リチウム等が好ましい。特に、無水セッコウが収縮向上の面から最も好ましい。
硫酸塩の粒度は、少量の配合量できのこの収率が向上することから細かいほど好ましい。具体的には、硫酸塩の平均粒子径は１mm以下が好ましく、100μｍ以下がより好ましく、10μｍ以下が最も好ましい。平均粒子径が１mmを越えると、きのこの収率の向上が得られない場合がある。
硫酸塩の配合量は、人工培養基100部中、0.01〜20部が好ましく、0.1 〜10部がより好ましい。0.01部未満あるいは20部を越えると、きのこの収率の向上が得られない場合がある。
【００１０】
本発明で使用する人工培養基としては、鋸屑、もみ殻、コーンコブミール、バガス、パルプ廃材、ビート粕、及びデンプン粕等の基材に、米糠、もろこし粉砕物、及びフスマ等の栄養源の一種又は二種以上の混合物にケイ酸カルシウム水和物、又はこれと硫酸塩を混合したものを使用することが可能である。
きのこの種類、栽培環境、及び条件等に応じて、基材や栄養源の種類、両者の配合割合は任意に変化するもので、特に限定されるものではないが、栄養源の使用量は、例えば、鋸屑等の基材100部に対して、10〜150部が、きのこを高収率で得る面からより好ましい。
【００１１】
本発明の人工培養基を用いてきのこを栽培する方法は、各々の環境や状況などに応じて任意に変えることができるので特に限定されるものではないが、通常、ケイ酸カルシウム水和物、又はこれと硫酸塩を混合した人工培養基に水を加えて、人工培養基の水分含有率を50〜70％に調整し、必要に応じて殺菌・冷却後、菌を接種し、各々のきのこについて通常採用されている培養工程や生育条件に従って行うことが好ましい。
例えば、ぶなしめじ栽培の場合は、菌を接種した人工培養基を22〜26℃で約30日間培養後、24〜28℃で40〜50日間熟成し、菌かき後に温度14〜17℃、湿度95〜100％で20〜25日間育成を行って収穫する。
また、しいたけ栽培の場合は、菌を接種した人工培養基を20〜25℃で約30日間培養後、26〜30℃で40〜50日間熟成し、その後、温度13〜17℃で１〜３日間低温処理し、温度17〜20℃、湿度90〜95％で約10日間発生を行ってきのこを収穫し、この際に第１回目の収穫後に再び発生にかけて第２回目の収穫を行うことも可能である。
【００１２】
本発明では、前述の基材や栄養源の他にも、必要に応じて人工培養基において使用されている、例えば、炭酸カルシウム、卵殻粉末、貝殻粉末、及び消石灰等の成分を併用することも可能である。
【００１３】
本発明で栽培されるきのこは人工栽培できるきのこであり、例えば、えのきたけ、ひらたけ、なめこ、ぶなしめじ、まいたけ、きくらげ、さるのこしかけ、及びしいたけ等が挙げられる。
【００１４】
【実施例】
以下、本発明の実験例を示し、本発明をさらに説明するが、本発明はこれらに限定されるものではない。
【００１５】
実験例１
広葉樹鋸屑250g、針葉樹鋸屑250g、米糠500g、及び水140mlをビニール袋に入れ充分に混合し、水分含有率65％の混合物を調製した。
調製した混合物とケイ酸カルシウム水和物からなる人工培養基100部中、表１に示すケイ酸カルシウム水和物ア〜オを３部添加混合した人工培養基500gをプラスチック製850mlの広口瓶に圧詰めした。
広口瓶の中央に直径約２cmの穴を開け、打栓後、120℃で90分間殺菌した。冷却後、ひらたけの鋸屑種菌を植菌し、暗所、温度25℃、湿度55％の条件下で30日間培養した。
次に、栓を外して培養基の上部から約１cm菌かきして菌糸層を除いた後、水道水20mlを添加して充分に吸水させた。４時間放置後、上部に残った水を取り除いて、温度15℃、湿度95％、照度20ルックスの条件下で、７日間培養して子実体原基を形成させ、さらに照度を200ルックスに上げて、10日間培養を続け、ケイ酸カルシウム水和物の組成が子実体収量におよぼす影響について検討した。結果を表１に併記する。
【００１６】
＜使用材料＞
広葉樹鋸屑：ぶな材の鋸屑
針葉樹鋸屑：すぎ材の鋸屑
米糠 ：市販品
ケイ酸カルシウム水和物ア：ゾノトライト、平均粒子径10μｍ
ケイ酸カルシウム水和物イ：ＣＳＨ（Ｉ）、平均粒子径10μｍ
ケイ酸カルシウム水和物ウ：ＣＳＨ（II）、平均粒子径10μｍ
ケイ酸カルシウム水和物エ：トバモライト、平均粒子径10μｍ
ケイ酸カルシウム水和物オ：ヒレブランダイト、平均粒子径10μｍ
【００１７】
＜測定方法＞
コントロール対比：（ケイ酸カルシウム水和物＋硫酸塩）添加の子実体収量(g)／（ケイ酸カルシウム水和物＋硫酸塩）無添加の子実体収量(g) ×100 (％)
【００１８】
【表１】
表１から明らかなように、人工培養基に、ケイ酸カルシウム水和物を含有することにより、ひらたけの収率が向上した。
【００２０】
実験例２
表２に示す量のケイ酸カルシウム水和物アを用いたこと以外は実験例１と同様に行った。結果を表２に併記する。
【００２１】
【表２】
表２から明らかなように、人工培養基中、ケイ酸カルシウム水和物が５部の場合、最もひらたけの収率が向上した。
【００２３】
実験例３
表３に示す平均粒子径のゾノトライトであるケイ酸カルシウム水和物を用いたこと以外は実験例１と同様に行った。結果を表３に併記する。
【００２４】
【表３】
表３から明らかなように、ケイ酸カルシウム水和物の平均粒子径が小さくなるほど、ひらたけの収率が向上した。
【００２６】
実験例４
人工培養基100部中、ケイ酸カルシウム水和物アを３部、表４に示す硫酸塩を３部としたこと以外は実験例１と同様に行った。結果を表４に併記する。
【００２７】
＜使用材料＞
硫酸塩ａ ：無水セッコウ、平均粒子径10μｍ
硫酸塩ｂ ：半水セッコウ、平均粒子径10μｍ
硫酸塩ｃ ：二水セッコウ、平均粒子径10μｍ
硫酸塩ｄ ：無水硫酸アルミニウム、平均粒子径10μｍ
硫酸塩ｅ ：硫酸アルミニウム18水塩、平均粒子径10μｍ
硫酸塩ｆ ：無水硫酸ナトリウム、平均粒子径10μｍ
硫酸塩ｇ ：無水硫酸マグネシウム、平均粒子径10μｍ
【００２８】
【表４】
表４から明らかなように、人工培養基中に、ケイ酸カルシウム水和物と硫酸塩を含有することにより、ひらたけの収率が向上した。
【００３０】
実験例５
コーンコブミール、米糠、及びフスマを重量比で５／４／１の割合で混合し、水分含有率63％の混合物を調製した。
人工培養基100部中、ケイ酸カルシウム水和物と硫酸塩ａを表５に示す量とした人工培養基700gを、プラスチック製1,100ml広口瓶に圧詰めした。
各々の中央に直径約２cmの穴を開け、打栓後、120℃で90分間殺菌した。冷却後、えのきたけの種菌を植菌し、温度18℃にて30日間培養した。
次に、菌かきをした後、芽出しを温度15℃、湿度95％で10日間、ならし・抑制を温度５〜８℃、湿度90％で７日間、収穫までの生育を温度５℃、湿度80％で栽培管理を行い30日後に収穫した。結果を表５に併記する。
なお、比較のため、ケイ酸カルシウム水和物の代わりにケイ酸カルシウムを２部使用して同様の実験を行った。結果を表５に併記する。
【００３１】
＜使用材料＞
ケイ酸カルシウムα：ウォラストナイト(β-CaO・SiO2)、平均粒子径10μｍ
ケイ酸カルシウムβ：3CaO・SiO2、平均粒子径10μｍ
【００３２】
【表５】
表５から明らかなように、人工培養基に、ケイ酸カルシウム水和物、又は、ケイ酸カルシウム水和物と硫酸塩とを含有することにより、えのきたけの収率が向上した。
【００３４】
【発明の効果】
以上、詳細に説明したとおり、本発明による栽培方法によれば、きのこを高収率で得ることが可能となった。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an artificial culture medium for mushrooms and an artificial cultivation method for mushrooms using the same.
In the present invention, “parts” and “%” are based on mass unless otherwise specified.
[0002]
[Prior art and its problems]
Conventionally, the cultivation of mushrooms has mostly been cultivating cypress trees using raw wood such as kunugi, beech, and nara. Therefore, there has been a problem that the harvest is often influenced by weather conditions.
[0003]
In addition, recently, there has been a problem that it is becoming difficult to obtain raw wood due to a lack of labor for cutting out raw wood for cultivating wood.
Furthermore, in the case of cultivating wood, since the cultivation period is long, that is, it takes one and a half years to two years from the inoculation of the inoculum to the harvest of the mushroom, there is a problem that the production cost is considerably high.
[0004]
In recent years, enokitake, hiratake, nameko, shiitake, etc. have established a fungus bed artificial cultivation method that uses an artificial culture medium in which rice bran is mixed with sawdust, and is cultivated in bottles or boxes, regardless of the seasons throughout the year. Mushrooms can be harvested stably.
In other words, mushroom cultivation, which used to be a farmer's sideline character and relied on small-scale production, is now shifting to a fungus bed artificial cultivation method that enables large-scale full-scale production and easy access to raw materials. .
However, even with the fungus bed artificial cultivation method, the yield is still low and the cultivation period is quite long to cultivate a large amount of mushrooms continuously. Improvement is eagerly desired.
For example, a compound represented by (Al 2 O 3 ) X (SiO 2 ) (where X is a number of 1 or more) is added to the artificial culture medium, or (MgO) W (Al 2 O 3 ) X (SiO 2 ) y (wherein W is a number of 1 to 3, X is a number of 1 to 5, and y is a number of 0 to 3) is contained in the above artificial culture medium. Or a material obtained by adding one kind of inorganic compound selected from aluminum silicate, calcium silicate, ferrous oxide, ferric oxide, and triiron tetroxide to the artificial culture medium. The present situation is that the yield cannot be produced (Japanese Patent Laid-Open Nos. 03-210126, 03-058716, and 07-322754).
[0005]
The present inventor found that mushrooms can be cultivated in high yield by using a specific artificial culture medium as a result of repeated sincerity studies in order to solve the problems of conventional methods in artificial cultivation of mushrooms, and the present invention It came to complete.
[0006]
[Means for Solving the Problems]
That is, the present invention is an artificial culture medium for mushrooms containing calcium silicate hydrate, wherein the calcium silicate hydrate has an average particle size of 10 μm or less, and the calcium silicate hydrate The mushroom artificial culture medium is characterized in that the amount used is 2 to 10 parts in 100 parts of the artificial culture medium, and further contains sulfate. And the calcium silicate hydrate is one or more kinds selected from zonotlite, CSH (II) and tobermorite, and is an artificial culture medium of the mushroom characterized by using these artificial culture media It is an artificial cultivation method for mushrooms.
[0007]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, the present invention will be described in more detail.
[0008]
Calcium silicate hydrate used in the present invention is necessary to improve the yield of mushrooms, silica raw materials such as quartzite, quartz sand, quartz, and diatomaceous earth, and quick lime (CaO) , Slaked lime (Ca (OH) 2 ), limestone (CaCO 3 ) and other calcia raw materials, which are obtained by hydrothermal synthesis. Specifically, zonotlite (6CaO · 6SiO 2 · H 2 O ), CSH (I), CSH (II), tobermorite (5CaO · 6SiO 2 · 1 to 9H 2 O), Hillebrandite (2CaO · SiO 2 · H 2 O), gyrolite, fossilite and other crystals And non-crystalline hydrates. Calcium silicate hydrate is produced in large quantities for building materials and heat insulating materials, and it is also possible to use materials generated in these production processes, end materials at construction sites, and the like. These may be used singly or in combination of two or more. The average particle size of the calcium silicate hydrate is preferably as small as possible since this yield can be improved by adding a small amount. Specifically, it is preferably 1 mm or less, more preferably 100 μm or less, and most preferably 10 μm or less. If it exceeds 1 mm, the improvement in the yield of mushrooms may not be obtained. The amount of calcium silicate hydrate used is preferably 0.01 to 20 parts, more preferably 2 to 10 parts, in 100 parts of the artificial culture medium. Outside this range, the yield of mushrooms may not be improved.
[0009]
In the present invention, the combined use of calcium silicate hydrate with sulfate is preferable from the viewpoint of further improving the yield of mushrooms.
Examples of sulfates include anhydrous gypsum, semi-aqueous gypsum, dihydrate gypsum, anhydrous aluminum sulfate, hydrous aluminum sulfate, anhydrous sodium sulfate, hydrous sodium sulfate, anhydrous magnesium sulfate, hydrous magnesium sulfate, anhydrous lithium sulfate, and hydrous lithium sulfate. preferable. In particular, anhydrous gypsum is most preferable from the viewpoint of improving shrinkage.
The finer the particle size of the sulfate, the better this yield, since a small amount can be added. Specifically, the average particle diameter of the sulfate is preferably 1 mm or less, more preferably 100 μm or less, and most preferably 10 μm or less. If the average particle size exceeds 1 mm, the yield of mushrooms may not be improved.
The blending amount of the sulfate is preferably 0.01 to 20 parts, more preferably 0.1 to 10 parts, in 100 parts of the artificial culture medium. If it is less than 0.01 part or exceeds 20 part, there is a case where the improvement of the mushroom yield cannot be obtained.
[0010]
As an artificial culture medium used in the present invention, a substrate such as sawdust, rice husk, corn cob meal, bagasse, pulp waste, beet lees, and starch lees, a kind of nutrient source such as rice bran, corn grind, and bran It is possible to use a mixture of two or more kinds of calcium silicate hydrate or a mixture thereof with sulfate.
Depending on the type of mushrooms, cultivation environment, conditions, etc., the type of base material and nutrient source, the mixing ratio of both may be arbitrarily changed, and although not particularly limited, the amount of nutrient source used is For example, with respect to 100 parts of the base material such as sawdust, 10 to 150 parts are more preferable from the viewpoint of obtaining mushrooms in a high yield.
[0011]
The method for cultivating mushrooms using the artificial culture medium of the present invention is not particularly limited because it can be arbitrarily changed according to each environment or situation, but usually calcium silicate hydrate, or Add water to the artificial culture medium mixed with this and sulfate to adjust the moisture content of the artificial culture medium to 50-70%, sterilize and cool as necessary, inoculate the fungus, and usually adopt for each mushroom It is preferable to carry out according to the culturing process and the growing conditions.
For example, in the case of Bunjimeji cultivation, the artificial culture medium inoculated with the fungus is cultured at 22-26 ° C. for about 30 days and then aged at 24-28 ° C. for 40-50 days. Harvest at ~ 100% for 20-25 days.
In the case of Shiitake cultivation, the artificial culture medium inoculated with the fungus is cultured at 20-25 ° C. for about 30 days, then aged at 26-30 ° C. for 40-50 days, and then at a temperature of 13-17 ° C. for 1-3 days. It is possible to harvest the mushrooms that have been generated for about 10 days at a temperature of 17-20 ° C and humidity of 90-95%. At this time, the second harvest can be performed after the first harvest. It is.
[0012]
In the present invention, in addition to the above-mentioned base materials and nutrient sources, components such as calcium carbonate, eggshell powder, shellfish powder, and slaked lime that are used in an artificial culture medium as necessary can be used in combination. It is.
[0013]
The mushrooms cultivated in the present invention are mushrooms that can be artificially cultivated, and examples thereof include enokitake mushrooms, octopus mushrooms, nameko mushrooms, bean mushrooms, maitake mushrooms, jellyfish mushrooms, and mushrooms.
[0014]
【Example】
Hereinafter, although the experiment example of this invention is shown and this invention is demonstrated further, this invention is not limited to these.
[0015]
Experimental example 1
250 g of hardwood sawdust, 250 g of softwood sawdust, 500 g of rice bran, and 140 ml of water were placed in a plastic bag and mixed well to prepare a mixture having a water content of 65%.
In 100 parts of the artificial culture medium consisting of the prepared mixture and calcium silicate hydrate, 500 g of the artificial culture medium mixed with 3 parts of calcium silicate hydrate a to o shown in Table 1 and mixed in a 850 ml plastic jar did.
A hole with a diameter of about 2 cm was made in the center of the wide-mouthed bottle, sterilized at 120 ° C. for 90 minutes after stoppering. After cooling, inoculum of scallop sawdust was inoculated and cultured for 30 days in the dark, at a temperature of 25 ° C. and a humidity of 55%.
Next, after removing the stopper and scraping about 1 cm of bacteria from the top of the culture medium to remove the mycelium layer, 20 ml of tap water was added to absorb water sufficiently. After standing for 4 hours, remove the water remaining in the upper part and incubate for 7 days under the conditions of temperature 15 ℃, humidity 95%, illumination 20 lux to form a fruiting body primordium, and further increase the illuminance to 200 lux The effect of the composition of calcium silicate hydrate on fruit body yield was examined. The results are also shown in Table 1.
[0016]
<Materials used>
Hardwood sawdust: Sawdust of beech coniferous sawdust: Sawdust of surplus wood: Commercially available calcium silicate hydrate A: Zonotolite, average particle size 10μm
Calcium silicate hydrate A: CSH (I), average particle size 10 μm
Calcium silicate hydrate C: CSH (II), average particle size 10μm
Calcium silicate hydrate D: Tobermorite, average particle size 10μm
Calcium silicate hydrate O: Hilleblandite, average particle size 10μm
[0017]
<Measurement method>
Control contrast: fruit body yield (g) with addition of (calcium silicate hydrate + sulfate) / fruit yield without addition of (calcium silicate hydrate + sulfate) (g) x 100 (%)
[0018]
[Table 1]
As is apparent from Table 1, the yield of octopus was improved by containing calcium silicate hydrate in the artificial culture medium.
[0020]
Experimental example 2
The experiment was conducted in the same manner as in Experimental Example 1 except that the amount of calcium silicate hydrate a shown in Table 2 was used. The results are also shown in Table 2.
[0021]
[Table 2]
As is clear from Table 2, when the amount of calcium silicate hydrate was 5 parts in the artificial culture medium, the yield of the octopus was most improved.
[0023]
Experimental example 3
The experiment was performed in the same manner as in Experimental Example 1 except that calcium silicate hydrate, which is a zonotolite having an average particle diameter shown in Table 3, was used. The results are also shown in Table 3.
[0024]
[Table 3]
As is apparent from Table 3, the yield of octopus was improved as the average particle size of the calcium silicate hydrate decreased.
[0026]
Experimental Example 4
It was carried out in the same manner as in Experimental Example 1 except that 100 parts of the artificial culture medium was composed of 3 parts of calcium silicate hydrate and 3 parts of sulfate shown in Table 4. The results are also shown in Table 4.
[0027]
<Materials used>
Sulfate a: anhydrous gypsum, average particle size 10 μm
Sulfate b: half water gypsum, average particle size 10μm
Sulfate c: dihydrate gypsum, average particle size 10 μm
Sulfate d: Anhydrous aluminum sulfate, average particle size 10 μm
Sulfate e: Aluminum sulfate 18 hydrate, average particle size 10μm
Sulfate f: anhydrous sodium sulfate, average particle size 10 μm
Sulfate g: anhydrous magnesium sulfate, average particle size 10μm
[0028]
[Table 4]
As is apparent from Table 4, the yield of octopus was improved by containing calcium silicate hydrate and sulfate in the artificial culture medium.
[0030]
Experimental Example 5
Corn cobmeal, rice bran, and bran were mixed at a weight ratio of 5/4/1 to prepare a mixture having a water content of 63%.
In 100 parts of the artificial culture medium, 700 g of the artificial culture medium with calcium silicate hydrate and sulfate a as shown in Table 5 was packed into a plastic 1,100 ml wide-mouth bottle.
A hole about 2 cm in diameter was made in the center of each, and after sterilization, sterilized at 120 ° C. for 90 minutes. After cooling, inoculum of Enokitake was inoculated and cultured at a temperature of 18 ° C. for 30 days.
Next, after mushrooming, germination is 15 ° C and humidity is 95% for 10 days. Cultivation was controlled at 80% and harvested 30 days later. The results are also shown in Table 5.
For comparison, the same experiment was conducted using 2 parts of calcium silicate instead of calcium silicate hydrate. The results are also shown in Table 5.
[0031]
<Materials used>
Calcium silicate α: Wollastonite (β-CaO · SiO 2 ), average particle size 10μm
Calcium silicate β: 3CaO · SiO 2 , average particle size 10μm
[0032]
[Table 5]
As is apparent from Table 5, the yield of oyster mushroom was improved by containing calcium silicate hydrate or calcium silicate hydrate and sulfate in the artificial culture medium.
[0034]
【The invention's effect】
As described above in detail, according to the cultivation method of the present invention, mushrooms can be obtained in high yield.
Claims (4)
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