JP4570296B2 - Mushroom artificial culture medium and mushroom artificial cultivation method using the same - Google Patents
Mushroom artificial culture medium and mushroom artificial cultivation method using the same Download PDFInfo
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- JP4570296B2 JP4570296B2 JP2001267085A JP2001267085A JP4570296B2 JP 4570296 B2 JP4570296 B2 JP 4570296B2 JP 2001267085 A JP2001267085 A JP 2001267085A JP 2001267085 A JP2001267085 A JP 2001267085A JP 4570296 B2 JP4570296 B2 JP 4570296B2
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- JP
- Japan
- Prior art keywords
- culture medium
- mushroom artificial
- hydroxide
- sawdust
- mushrooms
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【0001】
【発明の属する技術分野】
本発明は、きのこの人工培養基及びそれを用いたきのこの人工栽培方法に関する。
なお、本発明でいう部や%は特に規定のないかぎり質量基準である。
【0002】
【従来の技術とその課題】
従来、きのこの栽培は、くぬぎ、ぶな、及びならなどの原木を利用したほだ木栽培がほとんどであり、そのため気象条件により収穫が左右されることが多い。
また、最近では、ほだ木栽培用の原木の切り出しのための労働力が不足していることなどによって、原木の入手が困難になりつつある。
さらに、ほだ木栽培では栽培期間が長いこと、即ち、種菌の接種からきのこの収穫までに1年半〜2年も要するので、生産コストが相当高くつくのが現状である。
【0003】
近年、えのきたけ、ひらたけ、なめこ、及びしいたけなどは、鋸屑に米糠を配合した人工培養基を用い、瓶又は箱で培養を行う菌床人工栽培方法が確立され、一年を通じて、四季に関係なく安定してこれらのきのこが収穫できるようになっている。
即ち、農家での副業的性格が強く、小規模生産に頼っていた従来のきのこの栽培が、現在では大規模専業生産が可能で、かつ、原料が入手しやすい菌床人工栽培方法に移りつつある。
【0004】
しかしながら、菌床人工栽培においても、きのこを大量に連続栽培するには、いまだ収率も低く、かつ、栽培期間がかなり長いため、その生産コストは安価とは言えず、今後、これら生産性の改善が切望されている。
【0005】
生産性を改善する方法としては、例えば、特定組成を有するアルミノシリケート系化合物や特定組成を有するマグネシウムアルミニウムシリケート系化合物を人工培養基中に含有させる方法が提案されている(特開平03-210126号公報、特開平03-58716号公報)。
【0006】
一方、リチウムアルミニウム複合水酸化物は、塩素吸着剤や制酸剤として利用され市販されており、その製造方法も各種提案されている(特開平05-179052号公報、特開平07-300313号公報、及び特開平10-17322号公報等)。
【0007】
しかしながら、これらの方法では充分な収率で生産することができていないのが実状である。
【0008】
本発明者は、きのこの人工栽培における課題を解消するために、鋭意努力を重ねた結果、特定の人工培養基を用いることによって、きのこが高収率で栽培できることを知見して本発明を完成するに至った。
【0009】
【課題を解決するための手段】
即ち、本発明は、リチウムアルミニウム複合水酸化物を含有してなるきのこの人工培養基であり、該人工培養基を用いてなるきのこの人工栽培方法である。
【0010】
【発明の実施の形態】
以下、本発明をさらに詳しく説明する。
【0011】
本発明で使用するリチウムアルミニウム複合水酸化物(以下、LA水酸化物という)とは、一般に、[Al2Li(OH)6]nX・mH2Oで表される化合物を総称するものである。式中のXは、例えば、炭酸イオン、重炭酸イオン、硫酸イオン、塩素イオン、塩化物イオン、硝酸イオン、亜硝酸イオン、及び水酸化物イオンなどのアニオンを表し、nは1又は2、mは0〜10である。
本発明ではいかなる組成を有するLA水酸化物も利用可能であるが、入手のしやすさから、Xが炭酸イオン、重炭酸イオン、又は水酸化物イオンであるものが好ましい。
LA水酸化物の平均粒径は、0.5〜20μm程度の範囲であり、そのBET比表面積は5〜70m2/g程度である。
【0012】
本発明で使用する人工培養基とは、通常、鋸屑、ふすま、及びもみがらなどの炭素源と、米糠や大豆粕等の窒素源との混合物、また、この混合物に水を適当量加え、これを瓶又は箱に圧詰めして調製したもの、さらに、これらにLA水酸化物を添加混合したものである。
【0013】
炭素源量、窒素源量、及び水量は、その種類や含有水分量等により変化し、一義的に決定されるものではない。
【0014】
例えば、炭素源として鋸屑を用いる場合、鋸屑は、乾燥状態で培地全乾物量の20〜90%程度の範囲で使用することができるが、この量は窒素源として用いる培地成分によって変動する。例えば、窒素源に米糠を使用する場合、鋸屑は乾物量として培地全乾物量の20〜90%の範囲で使用するが、鋸屑と米糠を重量比で1:1で混合した混合物に水を加えて、水分含水率を55〜70%に調整したものを、瓶又は箱に圧詰めして調製することが好ましい。
【0015】
また、鋸屑としては広葉樹鋸屑あるいは針葉樹鋸屑をそれぞれ単独あるいは混合して使用することも可能である。
【0016】
LA水酸化物の混合割合は、炭素源と窒素源からなる人工培養基100部に対して、1〜30部が好ましく、3〜15部がより好ましい。LA水酸化物の混合割合がこの範囲外では本発明の効果が充分に得られない場合がある。
【0017】
本発明に係るきのこは、人工栽培できるきのこであれば特に限定されるものではない。その具体例としては、例えば、えのきたけ、ひらたけ、なめこ、ぶなしめじ、及びしいたけなどが挙げられる。
【0018】
本発明では、LA水酸化物、鋸屑等の炭素源、米糠等の窒素源、及び水のほかに、従来より利用されている、きのこの収率を向上させる材料である、アルミノシリケート系化合物、マグネシウムアルミニウムシリケート系化合物、マグネシウムシリケート系化合物、カルシウムシリケート系化合物、マグネシウムアルミネート系化合物、カルシウムアルミネート系化合物、スラグ粉末、エトリンガイト、リン化合物、水酸化カルシウム、及び炭酸カルシウムなどを本発明の目的を実質的に阻害しない範囲で併用することが可能である。
【0019】
【実施例】
以下、実験例により本発明を詳細に説明する。
【0020】
実験例1
広葉樹鋸屑500g、針葉樹鋸屑500g、及び米糠1,000gをビニール袋に入れ充分に混合して人工培養基を調製した。
調製した人工培養基200gに表1に示すLA水酸化物を添加し、水分含有率を65%に調整して混合物とし、その混合物200gをプラスチック製1リットル広口瓶に圧詰めした。それぞれの瓶の中央に直径1cm程度の穴を開け、打栓後、120℃で90分間殺菌した。
冷却後、ひらたけの鋸屑菌種を植菌し、暗所で温度25℃、相対湿度55%の条件下で30日間培養(菌まわし工程)し、さらに、30日間培養を続けて熟成させた。
次に、栓を外して培養基の上部から約1cm程度菌かきをして菌糸層を除いた後、水道水20mlを添加して充分に吸水させた。
4時間放置後、上部に残った水を取り除いて、温度15℃、相対湿度95%、及び照度20ルクスの条件下で、4日間培養して子実体原基を形成させ、さらに照度を200ルクスに上げて、10日間培養を続け、LA水酸化物の重量比が子実体収量におよぼす影響について検討した。結果を表1に併記する。
【0021】
<使用材料>
広葉樹鋸屑:ぶな材の鋸屑
針葉樹鋸屑:すぎ材の鋸屑
米糠 :市販品
LA水酸化物:市販品
水 :水道水
【0022】
<測定方法>
コントロール対比:LA水酸化物添加の人工培養基の子実体収量(g)/LA水酸化物無添加の人工培養基の子実体収量(g)×100(%)
【0023】
【表1】
【0024】
【発明の効果】
本発明のきのこの人工培養基を用いることによって、きのこの収率を飛躍的に高めることができる。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an artificial culture medium for mushrooms and an artificial cultivation method for mushrooms using the same.
In the present invention, “parts” and “%” are based on mass unless otherwise specified.
[0002]
[Prior art and its problems]
Conventionally, mushroom cultivation is mostly mortar cultivation using raw wood such as kunugi, beech and nara, and therefore the harvest is often influenced by weather conditions.
In addition, recently, it has become difficult to obtain raw wood due to a lack of labor for cutting out raw wood for cultivating wood.
In addition, the cultivation period is long in hodoki cultivation, that is, it takes one and a half years to two years from the inoculation of the inoculum to the harvest of the mushrooms, so that the production cost is considerably high at present.
[0003]
In recent years, enokitake, hiratake, nameko, shiitake, etc., have been established for artificial cultivation of fungus beds using a culture medium containing sawdust and rice bran in bottles or boxes, regardless of the season throughout the year. These mushrooms can be harvested stably.
In other words, the traditional mushroom cultivation, which has a strong side-business character at farmers and relies on small-scale production, is now shifting to a fungus bed artificial cultivation method that allows large-scale specialized production and is easy to obtain raw materials. is there.
[0004]
However, even in fungus bed artificial cultivation, in order to continuously cultivate mushrooms in large quantities, the yield is still low and the cultivation period is quite long, so the production cost cannot be said to be low. Improvement is eagerly desired.
[0005]
As a method for improving productivity, for example, a method in which an aluminosilicate compound having a specific composition or a magnesium aluminum silicate compound having a specific composition is contained in an artificial culture medium has been proposed (Japanese Patent Laid-Open No. 03-210126). JP, 03-58716, A).
[0006]
On the other hand, lithium aluminum composite hydroxide is commercially available as a chlorine adsorbent or an antacid, and various production methods have been proposed (Japanese Patent Laid-Open Nos. 05-179052 and 07-300313). And JP-A-10-17322).
[0007]
However, the fact is that these methods cannot be produced in a sufficient yield.
[0008]
The present inventor completed the present invention by finding that mushrooms can be cultivated in high yield by using a specific artificial culture medium as a result of intensive efforts to solve the problems in artificial cultivation of mushrooms. It came to.
[0009]
[Means for Solving the Problems]
That is, the present invention is an artificial culture medium for mushrooms containing a lithium aluminum composite hydroxide, and an artificial cultivation method for mushrooms using the artificial culture medium.
[0010]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, the present invention will be described in more detail.
[0011]
The lithium aluminum composite hydroxide (hereinafter referred to as LA hydroxide) used in the present invention is a general term for compounds represented by [Al 2 Li (OH) 6 ] n X · mH 2 O. is there. X in the formula represents anions such as carbonate ion, bicarbonate ion, sulfate ion, chloride ion, chloride ion, nitrate ion, nitrite ion, and hydroxide ion, and n is 1 or 2, m Is 0-10.
In the present invention, LA hydroxide having any composition can be used, but from the viewpoint of availability, X is preferably a carbonate ion, bicarbonate ion, or hydroxide ion.
The average particle size of the LA hydroxide is in the range of about 0.5 to 20 μm, and the BET specific surface area is about 5 to 70 m 2 / g.
[0012]
The artificial culture medium used in the present invention is usually a mixture of a carbon source such as sawdust, bran and rice bran and a nitrogen source such as rice bran and soybean meal, and an appropriate amount of water is added to this mixture. It is prepared by crushing into a bottle or box, and further, LA hydroxide is added to and mixed with these.
[0013]
The amount of carbon source, the amount of nitrogen source, and the amount of water vary depending on the type, the amount of water contained, etc., and are not uniquely determined.
[0014]
For example, when sawdust is used as the carbon source, the sawdust can be used in a range of about 20 to 90% of the total amount of dry matter in the dry state, but this amount varies depending on the medium component used as the nitrogen source. For example, when rice bran is used as a nitrogen source, sawdust is used as a dry matter amount in the range of 20 to 90% of the total dry matter of the medium, but water is added to a mixture of sawdust and rice bran in a weight ratio of 1: 1. Thus, it is preferable to prepare by adjusting the water content of water to 55 to 70% in a bottle or box.
[0015]
Moreover, as sawdust, hardwood sawdust or coniferous sawdust can be used alone or in combination.
[0016]
The mixing ratio of LA hydroxide is preferably 1 to 30 parts, more preferably 3 to 15 parts with respect to 100 parts of the artificial culture medium composed of a carbon source and a nitrogen source. When the mixing ratio of the LA hydroxide is outside this range, the effects of the present invention may not be sufficiently obtained.
[0017]
The mushroom according to the present invention is not particularly limited as long as it can be artificially cultivated. Specific examples thereof include enokitake, hiratake, nameko, bean shimeji, shiitake and the like.
[0018]
In the present invention, in addition to LA hydroxide, a carbon source such as sawdust, a nitrogen source such as rice bran, and water, an aluminosilicate compound, which is a conventionally used material for improving the yield of mushrooms, The object of the present invention is magnesium aluminum silicate compound, magnesium silicate compound, calcium silicate compound, magnesium aluminate compound, calcium aluminate compound, slag powder, ettringite, phosphorus compound, calcium hydroxide, calcium carbonate, etc. It can be used in combination as long as it does not substantially inhibit.
[0019]
【Example】
Hereinafter, the present invention will be described in detail by experimental examples.
[0020]
Experimental example 1
An artificial culture medium was prepared by placing 500 g of hardwood sawdust, 500 g of coniferous sawdust and 1,000 g of rice bran in a plastic bag and mixing them well.
LA hydroxide shown in Table 1 was added to 200 g of the prepared artificial culture medium, the water content was adjusted to 65% to obtain a mixture, and 200 g of the mixture was pressed into a plastic 1-liter wide-mouth bottle. A hole with a diameter of about 1 cm was made in the center of each bottle and after sterilization, the bottle was sterilized at 120 ° C for 90 minutes.
After cooling, scallop sawdust species were inoculated, cultured in the dark at a temperature of 25 ° C. and a relative humidity of 55% for 30 days (fungus spinning process), and further cultured for 30 days for aging.
Next, the stopper was removed, and about 1 cm of bacteria was scraped from the top of the culture medium to remove the mycelium layer, and then 20 ml of tap water was added to absorb water sufficiently.
After standing for 4 hours, remove the water remaining on the top and incubate for 4 days under the conditions of temperature 15 ° C, relative humidity 95% and illuminance 20 lux to form a fruiting body primordium, and illuminance is 200 lux. The culture was continued for 10 days, and the influence of the weight ratio of LA hydroxide on fruit body yield was examined. The results are also shown in Table 1.
[0021]
<Materials used>
Hardwood Sawdust: Beechwood Sawdust Coniferous Sawdust: Sawwood Sawdust Rice Butter: Commercial Product LA Hydroxide: Commercial Product Water: Tap Water [0022]
<Measurement method>
Control contrast: fruit body yield of artificial culture medium with LA hydroxide added (g) / fruit body yield of artificial culture medium without LA hydroxide added (g) x 100 (%)
[0023]
[Table 1]
[0024]
【The invention's effect】
By using the artificial culture medium for mushrooms of the present invention, the yield of mushrooms can be dramatically increased.
Claims (2)
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0284112A (en) * | 1987-11-19 | 1990-03-26 | Takara Shuzo Co Ltd | Artificial cultivation of mushroom |
JPH03210126A (en) * | 1990-01-11 | 1991-09-13 | Takara Shuzo Co Ltd | Method for artificially culturing mushroom |
JPH0466029A (en) * | 1990-07-06 | 1992-03-02 | Fuji Kagaku Kogyo Kk | Soil cover for cultivating mushroom and cultivation of mushroom |
JPH11187762A (en) * | 1997-12-26 | 1999-07-13 | Denki Kagaku Kogyo Kk | Artificial culture medium for mushroom and artificial culture for mushroom using the same |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6434216A (en) * | 1987-07-31 | 1989-02-03 | Nisshin Flour Milling Co | Culture medium for mushroom |
-
2001
- 2001-09-04 JP JP2001267085A patent/JP4570296B2/en not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0284112A (en) * | 1987-11-19 | 1990-03-26 | Takara Shuzo Co Ltd | Artificial cultivation of mushroom |
JPH03210126A (en) * | 1990-01-11 | 1991-09-13 | Takara Shuzo Co Ltd | Method for artificially culturing mushroom |
JPH0466029A (en) * | 1990-07-06 | 1992-03-02 | Fuji Kagaku Kogyo Kk | Soil cover for cultivating mushroom and cultivation of mushroom |
JPH11187762A (en) * | 1997-12-26 | 1999-07-13 | Denki Kagaku Kogyo Kk | Artificial culture medium for mushroom and artificial culture for mushroom using the same |
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