JP4447459B2 - 原核生物dnaを分離する方法、体液を精製する方法および原核生物dnaを検出する方法、ならびに原核生物dnaを検出するための試験キット - Google Patents

原核生物dnaを分離する方法、体液を精製する方法および原核生物dnaを検出する方法、ならびに原核生物dnaを検出するための試験キット Download PDF

Info

Publication number
JP4447459B2
JP4447459B2 JP2004542300A JP2004542300A JP4447459B2 JP 4447459 B2 JP4447459 B2 JP 4447459B2 JP 2004542300 A JP2004542300 A JP 2004542300A JP 2004542300 A JP2004542300 A JP 2004542300A JP 4447459 B2 JP4447459 B2 JP 4447459B2
Authority
JP
Japan
Prior art keywords
dna
prokaryotic dna
prokaryotic
separating
tlr9
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2004542300A
Other languages
English (en)
Japanese (ja)
Other versions
JP2005538737A (ja
JP2005538737A5 (enExample
Inventor
シュミット、カール−ヘルマン
シュトラウベ、エーバーハルト
ルースブルム、シュテファン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SIRS Lab GmbH
Original Assignee
SIRS Lab GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SIRS Lab GmbH filed Critical SIRS Lab GmbH
Publication of JP2005538737A publication Critical patent/JP2005538737A/ja
Publication of JP2005538737A5 publication Critical patent/JP2005538737A5/ja
Application granted granted Critical
Publication of JP4447459B2 publication Critical patent/JP4447459B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2004542300A 2002-09-18 2003-08-08 原核生物dnaを分離する方法、体液を精製する方法および原核生物dnaを検出する方法、ならびに原核生物dnaを検出するための試験キット Expired - Fee Related JP4447459B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02020904A EP1400589B1 (de) 2002-09-18 2002-09-18 Verfahren zum Nachweis prokaryontischer DNA
PCT/EP2003/008825 WO2004033683A1 (de) 2002-09-18 2003-08-08 Verfahren zur anreicherung prokaryontischer dna

Publications (3)

Publication Number Publication Date
JP2005538737A JP2005538737A (ja) 2005-12-22
JP2005538737A5 JP2005538737A5 (enExample) 2006-09-21
JP4447459B2 true JP4447459B2 (ja) 2010-04-07

Family

ID=31896878

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2004542300A Expired - Fee Related JP4447459B2 (ja) 2002-09-18 2003-08-08 原核生物dnaを分離する方法、体液を精製する方法および原核生物dnaを検出する方法、ならびに原核生物dnaを検出するための試験キット

Country Status (12)

Country Link
US (2) US8062854B2 (enExample)
EP (1) EP1400589B1 (enExample)
JP (1) JP4447459B2 (enExample)
KR (1) KR20050057152A (enExample)
CN (1) CN1681925A (enExample)
AT (1) ATE335816T1 (enExample)
AU (1) AU2003260392A1 (enExample)
CA (1) CA2498951C (enExample)
DE (1) DE50207801D1 (enExample)
DK (1) DK1400589T3 (enExample)
ES (1) ES2266374T3 (enExample)
WO (1) WO2004033683A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE335816T1 (de) 2002-09-18 2006-09-15 Sirs Lab Gmbh Verfahren zum nachweis prokaryontischer dna
ES2384571T3 (es) * 2004-03-05 2012-07-09 Sirs-Lab Gmbh Procedimiento para el enriquecimiento y/o la separación de ADN procariota mediante una proteína que se une específicamente a ADN que contiene motivos CpG no metilados
DE102005001889B4 (de) * 2005-01-14 2012-03-29 Sirs-Lab Gmbh Verfahren zur Steigerung der Bindungskapazität und -effizienz eines Proteins, das nicht methylierte CPG-Motive enthaltende DNA spezifisch bindet
GB0413688D0 (en) * 2004-06-18 2004-07-21 Novartis Forschungsstiftung Analysis of methylated nucleic acid
DE202005009490U1 (de) * 2005-06-16 2006-10-19 Sirs-Lab Gmbh Vorrichtung zur Anreicherung/Abtrennung von nicht-methylierte CpG-Motive enthaltender DNA
DE102005034628B4 (de) * 2005-07-19 2007-08-23 Epigenomics Ag Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA
DE102007041864B4 (de) 2007-09-04 2012-05-03 Sirs-Lab Gmbh Verfahren zum Nachweis von Bakterien und Pilzen
LT3067431T (lt) 2010-09-15 2019-11-11 Debiopharm Int Sa Įrenginys, turintis kraujo komponentus, skirtas atskirti tikslines molekules ar daleles iš mėginių
US9145580B2 (en) * 2011-04-02 2015-09-29 New England Biolabs, Inc. Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides
US8980553B2 (en) * 2011-04-02 2015-03-17 New England Biolabs, Inc. Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides
DE102012219142B4 (de) 2012-10-19 2016-02-25 Analytik Jena Ag Verfahren zum Trennen, Erfassen oder Anreichern von unterschiedlichen DNA-Spezies
WO2020216966A1 (en) * 2019-04-26 2020-10-29 Thermo Fisher Scientific Baltics Uab Isolated nucleic acid binding domains

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6239116B1 (en) 1994-07-15 2001-05-29 University Of Iowa Research Foundation Immunostimulatory nucleic acid molecules
JPH11510692A (ja) * 1995-07-28 1999-09-21 ベス イスラエル ディーコネス メディカル センター,インク. 突然変異誘発を検査するための方法及び検査器具
WO2001012659A2 (en) 1999-08-18 2001-02-22 Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. Human dna sequences
JP2002034565A (ja) 2000-07-19 2002-02-05 Japan Science & Technology Corp 細菌dnaを特異的に認識する受容体タンパク質
ATE335816T1 (de) 2002-09-18 2006-09-15 Sirs Lab Gmbh Verfahren zum nachweis prokaryontischer dna
ES2384571T3 (es) * 2004-03-05 2012-07-09 Sirs-Lab Gmbh Procedimiento para el enriquecimiento y/o la separación de ADN procariota mediante una proteína que se une específicamente a ADN que contiene motivos CpG no metilados
GB0423991D0 (en) * 2004-10-29 2004-12-01 Univ Edinburgh Applications of nucleic acid fragments

Also Published As

Publication number Publication date
AU2003260392A1 (en) 2004-05-04
US20100316993A1 (en) 2010-12-16
KR20050057152A (ko) 2005-06-16
EP1400589A1 (de) 2004-03-24
CA2498951C (en) 2013-04-23
JP2005538737A (ja) 2005-12-22
ES2266374T3 (es) 2007-03-01
CN1681925A (zh) 2005-10-12
DK1400589T3 (da) 2006-11-27
DE50207801D1 (de) 2006-09-21
EP1400589B1 (de) 2006-08-09
US8062854B2 (en) 2011-11-22
US20120040363A1 (en) 2012-02-16
CA2498951A1 (en) 2004-04-22
WO2004033683A1 (de) 2004-04-22
ATE335816T1 (de) 2006-09-15
US8288115B2 (en) 2012-10-16

Similar Documents

Publication Publication Date Title
US8288115B2 (en) Method for enriching a prokaryotic DNA
EP2505666B1 (en) Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides
US20190225958A1 (en) Method for enrichment and purification of cell-free dna from body fluid for high-throughput processing
JP2018504089A (ja) ピペットチップおよびその使用方法
JP4848358B2 (ja) 非メチル化CpGモチーフを含むDNAに特異的に結合するタンパク質を用いて原核生物DNAの濃縮または分離のうち少なくともいずれか一方を行う方法
CN111868253A (zh) 提取核酸的方法
CN106232812A (zh) 短链核酸的回收方法
KR20180052991A (ko) 살모넬라 타이피뮤리움 생균의 표면에 특이적으로 결합하는 dna 앱타머 및 이의 용도
JP2007525982A5 (enExample)
CN113227363B (zh) 腺病毒类型的特异性dna适体
EP2171098B1 (en) Methods for extraction and purification of components of biological samples
RU2603098C1 (ru) Способ выделения циркулирующих днк из плазмы или сыворотки крови
EP1475387B1 (en) Method for preparing assay samples
JP3800615B2 (ja) 核酸診断用粒子の調製方法および該核酸診断用粒子を用いた検査試料中の標的核酸の検出方法
CN115948407B (zh) 丁香假单胞杆菌猕猴桃致病变种适配体及筛选方法和应用
JP4519247B2 (ja) 核酸の抽出精製用試薬
JP2006042743A (ja) 細菌の回収方法
CN113980970A (zh) 与海葵溶细胞素gt-4特异性结合的适配体及其应用
CN116804196A (zh) 一种用于检测hcmv病毒的核酸适配体、试剂盒及应用
JPH11142409A (ja) 病原性微生物の検出方法
US20110224420A1 (en) Solid support
CN120989070A (zh) 基于噬菌体裂解酶突变体的mrsa及其耐药基因核酸poct快速检测试剂盒、快速提取纯化试剂和3重pcr快速扩增检测试剂
JPH09322777A (ja) 固相担体を用いる生理活性物質の処理方法
CN118207301A (zh) 一种基于带表面尖刺的二氧化硅纳米球高效捕获微量dna联合pcr原位扩增的方法和应用
CN119592714A (zh) 细菌16s扩增试剂盒、检测方法及应用

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20060802

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20060802

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20090602

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20090902

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20090909

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20091002

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20091009

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20091202

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20100105

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20100120

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130129

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130129

Year of fee payment: 3

S531 Written request for registration of change of domicile

Free format text: JAPANESE INTERMEDIATE CODE: R313531

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130129

Year of fee payment: 3

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20140129

Year of fee payment: 4

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees