ES2266374T3 - Procedimiento para la deteccion de adn procariotico. - Google Patents
Procedimiento para la deteccion de adn procariotico. Download PDFInfo
- Publication number
- ES2266374T3 ES2266374T3 ES02020904T ES02020904T ES2266374T3 ES 2266374 T3 ES2266374 T3 ES 2266374T3 ES 02020904 T ES02020904 T ES 02020904T ES 02020904 T ES02020904 T ES 02020904T ES 2266374 T3 ES2266374 T3 ES 2266374T3
- Authority
- ES
- Spain
- Prior art keywords
- dna
- protein
- polypeptide
- baselineskip
- prokaryotic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 70
- 238000001514 detection method Methods 0.000 title claims abstract description 39
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 65
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 60
- 229920001184 polypeptide Polymers 0.000 claims abstract description 44
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 44
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 44
- 108020004414 DNA Proteins 0.000 claims description 136
- 210000001124 body fluid Anatomy 0.000 claims description 27
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- 238000000338 in vitro Methods 0.000 claims description 6
- 230000011987 methylation Effects 0.000 claims description 6
- 238000007069 methylation reaction Methods 0.000 claims description 6
- 101000947157 Homo sapiens CXXC-type zinc finger protein 1 Proteins 0.000 claims description 5
- 230000003321 amplification Effects 0.000 claims description 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 5
- 238000000746 purification Methods 0.000 claims description 5
- 101150038509 TLR9 gene Proteins 0.000 claims description 3
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- 244000052769 pathogen Species 0.000 description 19
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- 230000003612 virological effect Effects 0.000 description 2
- 239000000304 virulence factor Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 108020000946 Bacterial DNA Proteins 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
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- 102000003839 Human Proteins Human genes 0.000 description 1
- 108010058683 Immobilized Proteins Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 206010051379 Systemic Inflammatory Response Syndrome Diseases 0.000 description 1
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- 238000004458 analytical method Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 244000078885 bloodborne pathogen Species 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000002612 cardiopulmonary effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
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- 230000002759 chromosomal effect Effects 0.000 description 1
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- 239000012149 elution buffer Substances 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
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- 239000011521 glass Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229940029575 guanosine Drugs 0.000 description 1
- 230000001951 hemoperfusion Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000003308 immunostimulating effect Effects 0.000 description 1
- 208000027866 inflammatory disease Diseases 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
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- 239000004816 latex Substances 0.000 description 1
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- 239000002184 metal Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000005373 porous glass Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- ZNNZYHKDIALBAK-UHFFFAOYSA-M potassium thiocyanate Chemical compound [K+].[S-]C#N ZNNZYHKDIALBAK-UHFFFAOYSA-M 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
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- 239000002683 reaction inhibitor Substances 0.000 description 1
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- 239000013049 sediment Substances 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 108010075210 streptolysin O Proteins 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 210000001179 synovial fluid Anatomy 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP02020904A EP1400589B1 (de) | 2002-09-18 | 2002-09-18 | Verfahren zum Nachweis prokaryontischer DNA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2266374T3 true ES2266374T3 (es) | 2007-03-01 |
Family
ID=31896878
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES02020904T Expired - Lifetime ES2266374T3 (es) | 2002-09-18 | 2002-09-18 | Procedimiento para la deteccion de adn procariotico. |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US8062854B2 (enExample) |
| EP (1) | EP1400589B1 (enExample) |
| JP (1) | JP4447459B2 (enExample) |
| KR (1) | KR20050057152A (enExample) |
| CN (1) | CN1681925A (enExample) |
| AT (1) | ATE335816T1 (enExample) |
| AU (1) | AU2003260392A1 (enExample) |
| CA (1) | CA2498951C (enExample) |
| DE (1) | DE50207801D1 (enExample) |
| DK (1) | DK1400589T3 (enExample) |
| ES (1) | ES2266374T3 (enExample) |
| WO (1) | WO2004033683A1 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE335816T1 (de) | 2002-09-18 | 2006-09-15 | Sirs Lab Gmbh | Verfahren zum nachweis prokaryontischer dna |
| ES2384571T3 (es) * | 2004-03-05 | 2012-07-09 | Sirs-Lab Gmbh | Procedimiento para el enriquecimiento y/o la separación de ADN procariota mediante una proteína que se une específicamente a ADN que contiene motivos CpG no metilados |
| DE102005001889B4 (de) * | 2005-01-14 | 2012-03-29 | Sirs-Lab Gmbh | Verfahren zur Steigerung der Bindungskapazität und -effizienz eines Proteins, das nicht methylierte CPG-Motive enthaltende DNA spezifisch bindet |
| GB0413688D0 (en) * | 2004-06-18 | 2004-07-21 | Novartis Forschungsstiftung | Analysis of methylated nucleic acid |
| DE202005009490U1 (de) * | 2005-06-16 | 2006-10-19 | Sirs-Lab Gmbh | Vorrichtung zur Anreicherung/Abtrennung von nicht-methylierte CpG-Motive enthaltender DNA |
| DE102005034628B4 (de) * | 2005-07-19 | 2007-08-23 | Epigenomics Ag | Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA |
| DE102007041864B4 (de) | 2007-09-04 | 2012-05-03 | Sirs-Lab Gmbh | Verfahren zum Nachweis von Bakterien und Pilzen |
| LT3067431T (lt) | 2010-09-15 | 2019-11-11 | Debiopharm Int Sa | Įrenginys, turintis kraujo komponentus, skirtas atskirti tikslines molekules ar daleles iš mėginių |
| US9145580B2 (en) * | 2011-04-02 | 2015-09-29 | New England Biolabs, Inc. | Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides |
| US8980553B2 (en) * | 2011-04-02 | 2015-03-17 | New England Biolabs, Inc. | Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides |
| DE102012219142B4 (de) | 2012-10-19 | 2016-02-25 | Analytik Jena Ag | Verfahren zum Trennen, Erfassen oder Anreichern von unterschiedlichen DNA-Spezies |
| WO2020216966A1 (en) * | 2019-04-26 | 2020-10-29 | Thermo Fisher Scientific Baltics Uab | Isolated nucleic acid binding domains |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6239116B1 (en) | 1994-07-15 | 2001-05-29 | University Of Iowa Research Foundation | Immunostimulatory nucleic acid molecules |
| JPH11510692A (ja) * | 1995-07-28 | 1999-09-21 | ベス イスラエル ディーコネス メディカル センター，インク． | 突然変異誘発を検査するための方法及び検査器具 |
| WO2001012659A2 (en) | 1999-08-18 | 2001-02-22 | Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. | Human dna sequences |
| JP2002034565A (ja) | 2000-07-19 | 2002-02-05 | Japan Science & Technology Corp | 細菌ｄｎａを特異的に認識する受容体タンパク質 |
| ATE335816T1 (de) | 2002-09-18 | 2006-09-15 | Sirs Lab Gmbh | Verfahren zum nachweis prokaryontischer dna |
| ES2384571T3 (es) * | 2004-03-05 | 2012-07-09 | Sirs-Lab Gmbh | Procedimiento para el enriquecimiento y/o la separación de ADN procariota mediante una proteína que se une específicamente a ADN que contiene motivos CpG no metilados |
| GB0423991D0 (en) * | 2004-10-29 | 2004-12-01 | Univ Edinburgh | Applications of nucleic acid fragments |
-
2002
- 2002-09-18 AT AT02020904T patent/ATE335816T1/de active
- 2002-09-18 DK DK02020904T patent/DK1400589T3/da active
- 2002-09-18 EP EP02020904A patent/EP1400589B1/de not_active Expired - Lifetime
- 2002-09-18 ES ES02020904T patent/ES2266374T3/es not_active Expired - Lifetime
- 2002-09-18 DE DE50207801T patent/DE50207801D1/de not_active Expired - Lifetime
-
2003
- 2003-08-08 WO PCT/EP2003/008825 patent/WO2004033683A1/de not_active Ceased
- 2003-08-08 AU AU2003260392A patent/AU2003260392A1/en not_active Abandoned
- 2003-08-08 JP JP2004542300A patent/JP4447459B2/ja not_active Expired - Fee Related
- 2003-08-08 US US10/528,235 patent/US8062854B2/en not_active Expired - Fee Related
- 2003-08-08 CA CA2498951A patent/CA2498951C/en not_active Expired - Fee Related
- 2003-08-08 KR KR1020057003719A patent/KR20050057152A/ko not_active Ceased
- 2003-08-08 CN CNA038222337A patent/CN1681925A/zh active Pending
-
2011
- 2011-10-05 US US13/253,661 patent/US8288115B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003260392A1 (en) | 2004-05-04 |
| US20100316993A1 (en) | 2010-12-16 |
| KR20050057152A (ko) | 2005-06-16 |
| EP1400589A1 (de) | 2004-03-24 |
| CA2498951C (en) | 2013-04-23 |
| JP2005538737A (ja) | 2005-12-22 |
| JP4447459B2 (ja) | 2010-04-07 |
| CN1681925A (zh) | 2005-10-12 |
| DK1400589T3 (da) | 2006-11-27 |
| DE50207801D1 (de) | 2006-09-21 |
| EP1400589B1 (de) | 2006-08-09 |
| US8062854B2 (en) | 2011-11-22 |
| US20120040363A1 (en) | 2012-02-16 |
| CA2498951A1 (en) | 2004-04-22 |
| WO2004033683A1 (de) | 2004-04-22 |
| ATE335816T1 (de) | 2006-09-15 |
| US8288115B2 (en) | 2012-10-16 |
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