CN1681925A - 原核dna的富集方法 - Google Patents
原核dna的富集方法 Download PDFInfo
- Publication number
- CN1681925A CN1681925A CNA038222337A CN03822233A CN1681925A CN 1681925 A CN1681925 A CN 1681925A CN A038222337 A CNA038222337 A CN A038222337A CN 03822233 A CN03822233 A CN 03822233A CN 1681925 A CN1681925 A CN 1681925A
- Authority
- CN
- China
- Prior art keywords
- dna
- protein
- described method
- polypeptide
- procaryotic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 66
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 61
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 51
- 229920001184 polypeptide Polymers 0.000 claims abstract description 44
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 42
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 42
- 108020004414 DNA Proteins 0.000 claims description 138
- 238000001514 detection method Methods 0.000 claims description 24
- 210000001124 body fluid Anatomy 0.000 claims description 21
- 239000010839 body fluid Substances 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 16
- 238000012360 testing method Methods 0.000 claims description 15
- 239000011159 matrix material Substances 0.000 claims description 12
- 230000003321 amplification Effects 0.000 claims description 8
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 8
- 239000002299 complementary DNA Substances 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 6
- 101000947157 Homo sapiens CXXC-type zinc finger protein 1 Proteins 0.000 claims description 4
- 101150038509 TLR9 gene Proteins 0.000 claims description 2
- 238000001962 electrophoresis Methods 0.000 claims description 2
- 230000004048 modification Effects 0.000 claims description 2
- 238000012986 modification Methods 0.000 claims description 2
- 230000001717 pathogenic effect Effects 0.000 description 26
- 238000003752 polymerase chain reaction Methods 0.000 description 22
- 210000004369 blood Anatomy 0.000 description 16
- 239000008280 blood Substances 0.000 description 16
- 239000003795 chemical substances by application Substances 0.000 description 16
- 239000000463 material Substances 0.000 description 14
- 239000000523 sample Substances 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 244000052616 bacterial pathogen Species 0.000 description 6
- 230000000813 microbial effect Effects 0.000 description 6
- 244000052769 pathogen Species 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000007857 nested PCR Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 4
- 238000013016 damping Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 150000007523 nucleic acids Chemical class 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000002560 therapeutic procedure Methods 0.000 description 4
- 108010067770 Endopeptidase K Proteins 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000007834 ligase chain reaction Methods 0.000 description 3
- 239000012139 lysis buffer Substances 0.000 description 3
- NCMVOABPESMRCP-SHYZEUOFSA-N 2'-deoxycytosine 5'-monophosphate Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)C1 NCMVOABPESMRCP-SHYZEUOFSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 102100036168 CXXC-type zinc finger protein 1 Human genes 0.000 description 2
- 230000004568 DNA-binding Effects 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 241000194046 Streptococcus intermedius Species 0.000 description 2
- 241000193996 Streptococcus pyogenes Species 0.000 description 2
- 108010060818 Toll-Like Receptor 9 Proteins 0.000 description 2
- 102100033117 Toll-like receptor 9 Human genes 0.000 description 2
- 108020005202 Viral DNA Proteins 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000004087 circulation Effects 0.000 description 2
- 230000001332 colony forming effect Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 230000001351 cycling effect Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229940124276 oligodeoxyribonucleotide Drugs 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 102000011931 Nucleoproteins Human genes 0.000 description 1
- 108010061100 Nucleoproteins Proteins 0.000 description 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 1
- 102000016979 Other receptors Human genes 0.000 description 1
- 101150102573 PCR1 gene Proteins 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 102000023732 binding proteins Human genes 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 230000008081 blood perfusion Effects 0.000 description 1
- 244000078885 bloodborne pathogen Species 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003302 ferromagnetic material Substances 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000003308 immunostimulating effect Effects 0.000 description 1
- 230000001524 infective effect Effects 0.000 description 1
- 208000027866 inflammatory disease Diseases 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 150000002500 ions Chemical group 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 230000005291 magnetic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- -1 particulate Substances 0.000 description 1
- 210000003516 pericardium Anatomy 0.000 description 1
- 210000004224 pleura Anatomy 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- ZNNZYHKDIALBAK-UHFFFAOYSA-M potassium thiocyanate Chemical compound [K+].[S-]C#N ZNNZYHKDIALBAK-UHFFFAOYSA-M 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 238000001814 protein method Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 108010075210 streptolysin O Proteins 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 210000001258 synovial membrane Anatomy 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP02020904.5 | 2002-09-18 | ||
| EP02020904A EP1400589B1 (de) | 2002-09-18 | 2002-09-18 | Verfahren zum Nachweis prokaryontischer DNA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1681925A true CN1681925A (zh) | 2005-10-12 |
Family
ID=31896878
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA038222337A Pending CN1681925A (zh) | 2002-09-18 | 2003-08-08 | 原核dna的富集方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US8062854B2 (enExample) |
| EP (1) | EP1400589B1 (enExample) |
| JP (1) | JP4447459B2 (enExample) |
| KR (1) | KR20050057152A (enExample) |
| CN (1) | CN1681925A (enExample) |
| AT (1) | ATE335816T1 (enExample) |
| AU (1) | AU2003260392A1 (enExample) |
| CA (1) | CA2498951C (enExample) |
| DE (1) | DE50207801D1 (enExample) |
| DK (1) | DK1400589T3 (enExample) |
| ES (1) | ES2266374T3 (enExample) |
| WO (1) | WO2004033683A1 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE335816T1 (de) | 2002-09-18 | 2006-09-15 | Sirs Lab Gmbh | Verfahren zum nachweis prokaryontischer dna |
| ES2384571T3 (es) * | 2004-03-05 | 2012-07-09 | Sirs-Lab Gmbh | Procedimiento para el enriquecimiento y/o la separación de ADN procariota mediante una proteína que se une específicamente a ADN que contiene motivos CpG no metilados |
| DE102005001889B4 (de) * | 2005-01-14 | 2012-03-29 | Sirs-Lab Gmbh | Verfahren zur Steigerung der Bindungskapazität und -effizienz eines Proteins, das nicht methylierte CPG-Motive enthaltende DNA spezifisch bindet |
| GB0413688D0 (en) * | 2004-06-18 | 2004-07-21 | Novartis Forschungsstiftung | Analysis of methylated nucleic acid |
| DE202005009490U1 (de) * | 2005-06-16 | 2006-10-19 | Sirs-Lab Gmbh | Vorrichtung zur Anreicherung/Abtrennung von nicht-methylierte CpG-Motive enthaltender DNA |
| DE102005034628B4 (de) * | 2005-07-19 | 2007-08-23 | Epigenomics Ag | Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA |
| DE102007041864B4 (de) | 2007-09-04 | 2012-05-03 | Sirs-Lab Gmbh | Verfahren zum Nachweis von Bakterien und Pilzen |
| LT3067431T (lt) | 2010-09-15 | 2019-11-11 | Debiopharm Int Sa | Įrenginys, turintis kraujo komponentus, skirtas atskirti tikslines molekules ar daleles iš mėginių |
| US9145580B2 (en) * | 2011-04-02 | 2015-09-29 | New England Biolabs, Inc. | Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides |
| US8980553B2 (en) * | 2011-04-02 | 2015-03-17 | New England Biolabs, Inc. | Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides |
| DE102012219142B4 (de) | 2012-10-19 | 2016-02-25 | Analytik Jena Ag | Verfahren zum Trennen, Erfassen oder Anreichern von unterschiedlichen DNA-Spezies |
| WO2020216966A1 (en) * | 2019-04-26 | 2020-10-29 | Thermo Fisher Scientific Baltics Uab | Isolated nucleic acid binding domains |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6239116B1 (en) | 1994-07-15 | 2001-05-29 | University Of Iowa Research Foundation | Immunostimulatory nucleic acid molecules |
| JPH11510692A (ja) * | 1995-07-28 | 1999-09-21 | ベス イスラエル ディーコネス メディカル センター，インク． | 突然変異誘発を検査するための方法及び検査器具 |
| WO2001012659A2 (en) | 1999-08-18 | 2001-02-22 | Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. | Human dna sequences |
| JP2002034565A (ja) | 2000-07-19 | 2002-02-05 | Japan Science & Technology Corp | 細菌ｄｎａを特異的に認識する受容体タンパク質 |
| ATE335816T1 (de) | 2002-09-18 | 2006-09-15 | Sirs Lab Gmbh | Verfahren zum nachweis prokaryontischer dna |
| ES2384571T3 (es) * | 2004-03-05 | 2012-07-09 | Sirs-Lab Gmbh | Procedimiento para el enriquecimiento y/o la separación de ADN procariota mediante una proteína que se une específicamente a ADN que contiene motivos CpG no metilados |
| GB0423991D0 (en) * | 2004-10-29 | 2004-12-01 | Univ Edinburgh | Applications of nucleic acid fragments |
-
2002
- 2002-09-18 AT AT02020904T patent/ATE335816T1/de active
- 2002-09-18 DK DK02020904T patent/DK1400589T3/da active
- 2002-09-18 EP EP02020904A patent/EP1400589B1/de not_active Expired - Lifetime
- 2002-09-18 ES ES02020904T patent/ES2266374T3/es not_active Expired - Lifetime
- 2002-09-18 DE DE50207801T patent/DE50207801D1/de not_active Expired - Lifetime
-
2003
- 2003-08-08 WO PCT/EP2003/008825 patent/WO2004033683A1/de not_active Ceased
- 2003-08-08 AU AU2003260392A patent/AU2003260392A1/en not_active Abandoned
- 2003-08-08 JP JP2004542300A patent/JP4447459B2/ja not_active Expired - Fee Related
- 2003-08-08 US US10/528,235 patent/US8062854B2/en not_active Expired - Fee Related
- 2003-08-08 CA CA2498951A patent/CA2498951C/en not_active Expired - Fee Related
- 2003-08-08 KR KR1020057003719A patent/KR20050057152A/ko not_active Ceased
- 2003-08-08 CN CNA038222337A patent/CN1681925A/zh active Pending
-
2011
- 2011-10-05 US US13/253,661 patent/US8288115B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003260392A1 (en) | 2004-05-04 |
| US20100316993A1 (en) | 2010-12-16 |
| KR20050057152A (ko) | 2005-06-16 |
| EP1400589A1 (de) | 2004-03-24 |
| CA2498951C (en) | 2013-04-23 |
| JP2005538737A (ja) | 2005-12-22 |
| JP4447459B2 (ja) | 2010-04-07 |
| ES2266374T3 (es) | 2007-03-01 |
| DK1400589T3 (da) | 2006-11-27 |
| DE50207801D1 (de) | 2006-09-21 |
| EP1400589B1 (de) | 2006-08-09 |
| US8062854B2 (en) | 2011-11-22 |
| US20120040363A1 (en) | 2012-02-16 |
| CA2498951A1 (en) | 2004-04-22 |
| WO2004033683A1 (de) | 2004-04-22 |
| ATE335816T1 (de) | 2006-09-15 |
| US8288115B2 (en) | 2012-10-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8288115B2 (en) | Method for enriching a prokaryotic DNA | |
| CN1187362C (zh) | 核酸的固相分离法 | |
| AU2019204844A1 (en) | Methods and kits for detecting cell-free pathogen-specific nucleic acids | |
| US10889868B2 (en) | Exosomal biomarkers diagnostic of tuberculosis | |
| CN1304589C (zh) | 利用固相载体从生物粒子中分离核酸的方法 | |
| JP3194943B2 (ja) | クリプトコックス・ネオホルマンスの検出に用いる核酸プローブおよび方法 | |
| Berdimuratova et al. | Optimization of PCR purification using silica-coated magnetic beads | |
| US20240025929A1 (en) | Methods and devices for ultrasensitive direct detection of microorganisms | |
| CN113136415A (zh) | 一种核酸提取方法及其用途 | |
| CN1717412A (zh) | 浓缩和纯化核酸的方法和装置 | |
| JP4684533B2 (ja) | 下層コロイド媒体からのフローティングにより生物サンプルを回収または除去する多段階法 | |
| CN110684824B (zh) | 一种用于检测布鲁氏菌抗原的免疫磁珠试剂盒 | |
| CN109852611B (zh) | 一种血细胞裂解液及利用该裂解液提取血液中核酸的方法 | |
| TW202323534A (zh) | 藉由固相吸附富集微生物核酸與鑑定微生物及/或抗藥基因的檢測系統及方法 | |
| JP3800615B2 (ja) | 核酸診断用粒子の調製方法および該核酸診断用粒子を用いた検査試料中の標的核酸の検出方法 | |
| CN1850991A (zh) | 猪附红细胞体pcr检测方法 | |
| CN1291039C (zh) | 一种提取样品中原核微生物dna的方法及其专用试剂 | |
| JP7725453B2 (ja) | 生物試料に由来する微生物ｒｎａの迅速な分離と収集 | |
| EP4558648A1 (en) | Methods and systems for detection and identification of pathogens and antibiotic resistance genes | |
| CN112813137A (zh) | 一种液体生物样品中选择性去除宿主核酸的方法 | |
| JPH11142409A (ja) | 病原性微生物の検出方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Open date: 20051012 |