WO2001012659A2 - Human dna sequences - Google Patents

Abstract

Novel human cDNA sequence of a clones, the encoded protein sequence of a clones, antibodies and variants thereof, are provided. The disclosed sequence of a clones find application in a number of ways, including use in profiling assays. In this regard, various assemblages of nucleic acids or proteins are provided that are useful in providing large arrays of human material for implementing large-scale screening strategies. The disclosed sequence of a clones may also be used in formulating medicaments, treating various disorders and in certain diagnostic applications.

Description

HUMAN DNA SEQUENCES

Background of the Invention

Current methods for testing pharmacological substances rely on a three-stage testing approach to drug development. First, candidate compounds are typically screened in some sort of in vitro system, like inhibition of cancer cell growth. Candidates are then tested in an animal model, as a first approximation of systemic effects, including efficacy and toxicity. Compounds that still show promise after these initial in vivo screens, finally are tested in humans. Again, human testing typically occurs in three phases: toxicity; preliminary efficacy; and efficacy. The entire process can take more than a decade and cost hundreds of millions of dollars. Aside from the monetary costs and protracted time scale, moreover, current testing regimes waste the lives of countless laboratory animals and needlessly endanger the lives of human subjects.

A need exists, therefore, for more sophisticated drug screening techniques that can be done rapidly in vitro. These screening techniques ideally will be reflective of systemic and/or organ-specific responses, so that they provide a reliable indicator of action in a human body. Current techniques, however, tend to utilize only a single or limited number of markers, thus answering only very simple questions that are of questionable medical import. For example, a typical in vitro assay may ask whether a lead compound binds a particular receptor, which has been implicated in a certain disorder. It is presumed that such binding is indicative of therapeutic usefulness, but it does not even purport to address systemic effects.

Not only are screening techniques for efficacy inadequate, the available toxicity screens likewise are inadequate. Toxicity, on a first level, is usually measured by animal testing. Aside from the complications related to in vivo versus in vitro testing, such screens are insufficient because of differences in metabolism, uptake, etc., relative to humans. Thus, improved methods would be not only be in v.trø-based, they would also be more "human. "

With the increasing miniaturization of screening assays and the growing availability of targets for pharmaceutical intervention, there is increasing interest in developing arrays containing large numbers of these targets that can be assayed simultaneously. If such an array contains a large enough population of targets, it can be used to essentially mimic the systemic response. In other words, the array becomes an in vitro surrogate for the human body. The more refined the array, the more accurate the predictive capability. In theory, an array could be constructed that can detect all of the known human expression products simultaneously, thereby, providing a very reliable indicator of the human response to a given compound. These arrays offer advantages over the present in vitro screening systems in that they can assay large numbers of responses simultaneously. They are superior to animal testing because they are more "human" and, thus, more predictive of human responses.

In order to construct such arrays, however, the field is in need of further human targets. Advantageously, such targets will be provided with additional physiologically relevant information, such as whether the target is expressed in a particular tissue and whether it is related to a known functional class of targets. In this way, the artisan can focus as needed, for example, on tissue-specific effects or target class-specific effects, thereby providing information useful in evaluating efficacy and/or toxicity.

In addition to a need for pharmacological screening targets, there is a need for further pharmacological substances. These substances can be used in the formulation of medicinal compositions and in treating a wide variety of disorders.

The present invention responds to the aforementioned and other needs in the field by providing a population of novel targets useful, inter alia, in the profiling and medicinal contexts described above.

Summary of the Invention

It is an object of the invention, therefore, to provide a set of human cDNA clones. Further to this object, the invention provides sequences of human cDNA clones that were isolated from libraries generated from different human tissues.

It is another object of the invention to provide assemblages of targets useful in profiling matrices for screening pharmacological test compounds. According to this object, assemblages comprising different populations of human nucleic acids, proteins and antibodies are provided. In different embodiments, cDNA library-specific assemblages and target-family-specific targets are provided. It is a further object of the invention to provide a database of human nucleotide and protein sequences. Further to this object, novel human nucleotide and protein sequences are provided in electronic form. In one embodiment, one or more of these sequences is provided in a searchable database.

It is still another object of the invention to provide biologically active target molecules useful in treating or detecting human disorders. Further to this object, the invention provides nucleic acid and protein molecules that have the capacity to affect disease etiology or symptoms or correlate with known disease states. Also further to this object, a database is provided which comprises the disclosed molecules in electronic form.

It is still a further object of the invention to provide polypeptides encoded by the human cDNA clones disclosed herein. Further to this object, the invention provides antibodies and fragments thereof that are capable of binding to a specific portion of these polypeptides.

It is yet another object of the invention to provide pharmaceutical compositions which comprise an effective amount of a pharmaceutical agent, wherein the pharmaceutical agent is selected from the group consisting of one or more polypeptides contemplated by the invention, variants or functional derivatives thereof, and antibodies thereto; and a physiologically acceptable carrier or excipient.

It is still another object of the invention to provide expression vectors comprising one or more human cDNA clones disclosed herein or fragments thereof; and optionally a promoter operably linked to the cDNA clone or fragment thereof . Further to this object, the invention provides methodology for recombinanfly producing a desired peptide, comprising expressing in a host cell a peptide encoded by a human cDNA clone disclosed herein.

Detailed Description

The invention results from a need in the art for new human nucleic acids and proteins. This need arises in several contexts. First, there is a need to identify targets for therapeutic intervention. Second, there is a need to identify molecules that may be adversely affected in a therapeutic context, thereby resulting in toxicity. Knowledge of these molecules will aid in the design of new medicaments with enhanced efficacy and decreased toxicity. Finally, the need encompasses human nucleic acids and proteins that have medicinal applicability in their own right.

In view of these needs, the present inventors set out to isolate and sequence human cDNAs from tissue-specific libraries. In this way, they represent subsets of molecules likely to be targets for therapeutic intervention or for avoiding toxicity. In addition, the inventors divided the molecules into various sub-categories, based on suspected functionality, structural similarity etc, which are of interest from a pharmacological perspective. These molecules are disclosed in provisional application serial nos. 60/149,499 and 60/156,503, filed August 18, 1999, and September 28, 1999, respectively, both of which are hereby incorporated by reference in their entirety.

GENERAL DESCRIPTION OF THE INVENTIVE MOLECULES

The present invention provides novel polynucleotide molecules that, in some instances, have similarities with known molecules. The inventive DNAs were cloned from five different human cDNA libraries. In addition to these DNA molecules, the invention provides their protein translations and antibodies derived from them. The inventive DNA and protein sequences are show individually, below. The inventive nucleic acids also include the complements of these DNA sequences, as well as their RNA counterparts. Methods of producing the molecules also are provided. Further, the invention provides methods for detecting all or part of the molecules and of detecting polynucleotides encoding all or part of the molecules.

The inventive molecules derive from five cDNA libraries: human fetal brain; human fetal kidney; human mammary carcinoma; human testis; and human uterus. For convenience, each sequence bears a designation that indicates from which library it is derived. In particular, these designations are: "hfpbr" for human fetal brain; "hfkd" for human fetal kidney; "hmcf" for human mammary carcinoma; "htes" for human testis; and "hute" for human uterus. The individual libraries were constructed and screened as described below in the examples.

The protein and DNA molecules of the invention are variously described herein as "target" molecules or "inventive" molecules. The sequences and other information pertinent to the nucleic acid and protein molecules of the invention are shown, below. Interpreting the data disclosed with the Table and cDNA sequences, below:

The table and data below provide the coding sequences of the inventive cDNAs as well as the protein sequences and other useful information, as set out below.

Grouping

The clones were assigned to the following fourteen functional and/or tissue-derived groups:

1. Cell Cycle

2. Cell Structure and Motility

3. Differentiation/Development

4. Intracellular Transport and Trafficking

5. Metabolism

6. Nucleic Acid Management

7. Signal Transduction

8. Transmembrane Protein

9. Transcription Factors

10. Brain derived

11. Kidney derived

12. Mammary Carcinoma derived

13. Testes derived

14. Uterus derived

Description of Clone Files

The individual clone files are structured in the same pattern. The Sections are separated by paragraphs.

1. Clone Name

The clone names are deciphered with reference to the following example: DKFZphfkd2_24e23, wherein the code represents:

• producer of library ("DKFZ") (for convenience, this reference may be eliminated)

• a "p" for "plasmid cDNA library" (for convenience, this reference may be eliminated)

• library name (e.g. hfbr = human fetal brain; hfkd = human fetal kidney; hmcf = human mammary carcinoma; htes = human testes; hute = human uterus) an underscore ("_") to separate library information from plate information plate number (e.g. "16") plate coordinates (letter first; e.g. "fl4")

Group 3. Introduction short review of the similarities, function of the protein and possible applications

4. Short Information specifications about the cDNA (who sequenced, completeness of the cDNA, similarity, who sequenced, chromosomal localisation, length of cDNA, localisation of poly A tail and polyadenylation signal)

5. cDNA-Sequence

6. BLASTn Results search results of blasting the cDNA sequence against all public databases

7. Medline Entries information about genes/proteins similar to the novel cDNA (if available)

8. Putative Encoded Protein Information specifications about the encoded protein (ORF: length and localisation of the reading frame)

9. Protein Sequence

10. BLASTp Results search results of blasting the protein sequence against all public databases

11. Pedant Information output of fully automated annotation: summarises peptide information, homologies, patterns as follows:

[Length]

- length of the protein = number of amino acid residues [MW] - molecular weight of the protein

[pi]

- isoelectric point

[HOMOL]

- shows protein with closest similarity to the cDNA-encoded protein [FUNCAT]

- functional information according to a catalogue developed by Munich Information center for Protein Sequences (MIPS)

[BLOCKS]

- Blocks are multiply aligned ungapped segments corresponding to the most highly conserved regions of proteins. The blocks for the Blocks Database are made automatically by looking for the most highly conserved regions in groups of proteins documented in the Prosite Database. The Prosite pattern for a protein group is not used in any way to make the Blocks Database and the pattern may or may not be contained in one of the blocks representing a group. These blocks are then calibrated against the SWISS-PROT database to obtain a measure of the chance distribution of matches. It is these calibrated blocks that make up the Blocks Database. The WWW versions of the Prosite and SWISS-PROT Databases that are used on this server are located at the ExPASy World Wide Web (WWW) Molecular Biology Server of the Geneva University Hospital and the University of Geneva. World Wide Web URL http://blocks.fhcrc.org/blocks/about_blocks.html/ is the entry point to the database.

- here Blocks segments found in the analysed protein sequences are displayed [SCOP]

Nearly all proteins have structural similarities with other proteins and, in some of these cases, share a common evolutionary origin. The scop database provides a detailed and comprehensive description of the structural and evolutionary relationships between all proteins whose structure is known, including all entries in Brookhaven National Laboratory's Protein Data Bank (PDB). It is available as a set of tightly linked hypertext documents which make the large database comprehensible and accessible. In addition, the hypertext pages offer a panoply of representations of proteins, including links to PDB entries, sequences, references, images and interactive display systems. World Wide Web URL http://scop.mrc-lmb.cam.ac.uk/scop/ is the entry point to the database. Existing automatic sequence and structure comparison tools cannot identify all structural and evolutionary relationships between proteins. The scop classification of proteins has been constructed manually by visual inspection and comparison of structures, but with the assistance of tools to make the task manageable and help provide generality. Proteins are classified to reflect both structural and evolutionary relatedness. Many levels exist in the hierarchy, but the principal levels are family, superfamily and fold. The exact position of boundaries between these levels are to some degree subjective. Scop evolutionary classification is generally conservative: where any doubt about relatedness exists, we made new divisions at the family and superfamily levels.

- - here SCOPE segments found in the analysed protein sequences are displayed

[EC]

ENZYME is a repository of information relative to the nomenclature of enzymes. It is primarily based on the recommendations of the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (IUBMB) and it describes each type of characterized enzyme for which an EC (Enzyme Commission) number has been provided. World Wide Web URL http://www.expasy.ch/enzyme/ is the entry point to the database.

- here EC -number and name of enzymes with similarity to the analysed protein sequences are displayed

[PIRKW]

- functional information according to the Protein Information Resource (PIR) database catalogue developed by Munich Information Center for Protein Sequences (MIPS), the National Biomedical Research Foundation (NBRF) and the International Protein Information Database in Japan (JIPID).

[SUPFAM]

- information according to the Protein Information Resource (PIR) database catalogue of protein superfamilies developed by Munich Information Center for Protein Sequences (MIPS), the National Biomedical Research Foundation (NBRF) and the International Protein Information Database in Japan (JIPID). [PROSITE] please refer to 12. PROSITE Motifs [PFAM] please refer to 13. PFAM Motifs [KW]

- overall 2dimensional folding information

- 3D indicates that the proteins is similar to a protein of which a 3 dimensional structure is known

- overall structural information

G

The last PEDANT-block depicts information about the folding structure of the protein generated by PREDATOR. PREDATOR is a secondary structure prediction program. It takes as input a single protein sequence to be predicted and can optimally use a set of unaligned sequences as additional information to predict the query sequence. The mean prediction accuracy of PREDATOR is 68% for a single sequence and 75% for a set of related sequences. PREDATOR does not use multiple sequence alignment. Instead, it relies on careful pairwise local alignments of the sequences in the set with the query sequence to be predicted.

World Wide Web URL http://www.embl- heidelberg.de/argos/predator/predator_info.html is the entry point to the database.

- H = helix, E - extended or sheet, _ = coil, T = transmembrane, B = beta

- x indicates a low-complexity region with repeat-like structure which is omitted in all BLAST searches

12. PROSITE Motifs

PROSITE is a database of protein families and domains. It consists of biologically significant sites, patterns and profiles that help to reliably identify to which known protein family (if any) a new sequence belongs. World Wide Web URL http://www.expasy.ch/prosite/ is the entry point to the database. A description of the prosite consensus patterns is also provided, below.

13. PFAM Motifs

PFAM (protein families) is a large collection of multiple sequence alignments and hidden Markov models covering many common protein domains. World Wide Web URL http://www.sanger.ac.uk/Pfam/ is the entry point to the database.

Deposit of Clones

Clones were deposited as a pool with the American Type Culture Collection under accession number , from which each clone comprising a particular polynucleotide is obtainable. Each clone has been transfected into separate bacterial cells (E. coli) in this composite deposit.

The clones may also be obtained from the Resource Center of the German Human Genome Project (Heubner Weg 6, 14059 Berlin, GERMANY). The Resource Center library numbers are slightly different that those presented here, but may be readily obtained by the following key or with the assistance of Resource Center personnel.

The library name becomes a number: brain (hfbr2) becomes 564; kidney (hfkd2) becomes 566; mammary carcinoma (hmcfl) becomes 727; testis (htes3) becomes 434;and uterus (hutel) becomes 586. Next, the plate number is converted to two digits (e.g., "2" becomes "02") and is moved behind the plate coordinate, and the underscore is dropped. The following examples are helpful:

Listed Number Resource Center Number

DKFZphfbr2_l 6f21 DKFZp564F2116

DKFZphfkd2_lj9 DKFZp566J091

DKFZphmcfl_lc23 DKFZp727C231

DKFZphtes3_14g5 DKFZp434G0514

DKFZphutel_17k7 DKFZp586K0717

The libraries were constructed using two commercially available vectors. The brain (hfbr2 designations) and kidney (hfkd2 designations) libraries utilize pAMP 1 from Life Technologies and are maintained in XL-2Blue (Strategene); the uterus (hutel), testes (htes3) and mammary carcinoma (hmcfl) libraries are constructed in pSPORTl, also from Life Technologies, and are maintained in DH10B (LifeTechnologies). In addition to the following techniques, consultation with the commercial literature available on these clones will make evident all of the housekeeping techniques needed to propagate and isolate the individual constructs. All inserts may be excised with a Notl/Sall digestion. Alternatively, universal primers, flanking the cloning region, may be used to amplify the inserts using PCR methods. Bacterial cells containing a particular clone can be obtained from the composite deposit as follows:

An oligonucleotide probe or probes should be designed to the sequence that is known for that particular clone. This sequence can be derived from the sequences provided herein, or from a combination of those sequences. Methods of probe design are presented below.

Oligonucleotide probes may be labeled with γ- ²P ATP (specific activity 6000 Ci/mmole) and T4 polynucleotide kinase using commonly employed techniques for labeling oligonucleotides. Other, non-radioactive labeling techniques can also be used. Unincorporated label typically is removed by gel filtration chromatography or other established methods. The amount of radioactivity incorporated into the probe can be quantified by measurement in a scintillation counter. Preferably, specific activity of the resulting probe generally should be approximately 4X10⁶ dmp/pmole.

The bacterial culture containing the pool of full-length clones should preferably be thawed and 100 μl of the stock used to inoculate a sterile culture flask containing 25 ml of sterile L-broth containing ampicillin at 50 - 100 μg/ml (for XL-2Blue strains 25 μg/ml tetracycline should also be used). The culture should preferably be grown to saturation at 37°C, and the saturated culture should preferably be diluted in fresh L-broth. Aliquots of these dilutions should preferably be plated to determine the dilution and volume which will yield approximately 5000 distinct and well-separated colonies on solid bacteriological media containing L-broth containing ampicillin at 100 μg/ml (for XL-2Blue strains 25 μg/ml tetracycline should also be used)and agar at l.5% in a l50 mm petri dish when grown overnight at 37°C. Other known methods of obtaining distinct, well-separated colonies can also be employed.

Standard colony hybridization procedures should then be used to transfer the colonies to nitrocellulose filters and lyse, denature and bake them. The filter is then preferably incubated at 65°C. for 1 hour with gentle agitation in 6 x SSC (20 x stock is 175.3 g NaCl/liter, 88.2 g Na citrate/liter, adjusted to pH 7.0 with NaOH) containing 0.5% SDS, 100 μg/ml of yeast RNA, and 10 mM EDTA (approximately 10 mL per 150 mm filter). Preferably, the probe is then added to the hybridization mix at a concentration greater than or equal to 1X10⁶ dpm/mL. The filter is then preferably incubated at 65°C. with gentle agitation overnight. The filter is then preferably washed in 500 mL of 2 x SSC/0.5% SDS at room temperature without agitation, preferably followed by 500 mL of 2 x SSC/0.1% SDS at room temperature with gentle shaking for 15 minutes. A third wash with 0.1 x SSC/0.5% SDS at 65°C. for 30 minutes to 1 hour is optional. The filter is then preferably dried and subjected to autoradiography for sufficient time to visualize the positives on the X-ray film. Other known hybridization methods can also be employed.

The positive colonies are picked, grown in culture, and plasmid DNA isolated using standard procedures. The clones can then be verified by restriction analysis, hybridization analysis, or DNA sequencing.

Alternatively, clones may be grown as described above, and PCR used to isolate the insert DNAs. Methods of PCR are described below and are otherwise well known .

ERROR SCREENING

The DNA sequences found herein derive from individual clones, which are publicly available, as noted above. Thus, the skilled artisan will recognize that any specific sequence disclosed herein readily can be screened for errors by resequencing a particular fragment, in both directions (i.e. , by sequencing both strands). Alternatively, error screening can be performed by amplifying and/or cloning any of the inventive DNAs, using for example RT- PCR, and sequencing the resulting amplified product. In the event that there is a sequencing error, reference should be made to the deposited clone as the correct sequence.

USES AND BIOLOGICAL ACTIVITIES OF THE INVENTIVE MOLECULES

The inventive molecules and their derivatives are susceptible to a wide variety of uses, based on functional and/or structural properties. The skilled worker will appreciate, based on the biological activities detailed below, and discussed with regard to the individual sequences disclosed below, that the inventive molecules will find usefulness in numerous therapeutic and diagnostic applications.

The DNA molecules, especially the potassium salts thereof, can be used as fertilizer supplements due to their high nitrogen and phosphorus contents. Since the DNAs are of defined length, they are also useful in gel electrophoresis as molecular weight markers. Due to their similarity with known molecules, certain of the DNA molecules and their variants and derivatives may be used in any number of different diagnostic procedures and therapeutic applications. They may also be used to make the encoded proteins. The proteins themselves have many possible uses. They may be used as a nutritional supplement for humans, animals and even for laboratory use as, for example, medium for bacterial cultures. Moreover, since the proteins are of defined, known sizes, they may be used as molecular weight markers for gel electrophoresis and gel filtration. Because they are of defined sequences, they also have use in microsequencing and protein fingerprinting applications.

Expression Profiling Applications

Given their known tissue expression and functional associations, assemblages of the inventive proteins (or corresponding antibodies) and nucleic acids are particularly suited to expression profiling applications. Expression profiling generally entails constructing an array of indicators that signal the presence of a particular RNA or protein expression product. Such arrays can be used to evaluate, for example, pharmacological effectiveness and toxicity. In particular, expression profiles from such arrays can be generated from cells treated with known compounds, having known properties, and these profiles can be compared to profiles of unknowns to evaluate similarities and differences, which can be correlated with efficacy or toxicity.

Additional uses of profiling include diagnosis, tracking development, and ascertaining signaling and metabolic pathways. For examples of references describing profiling and its uses, see Farr et α/., U.S. Patent 5,811,231 (1998); Seilhamer et al. , U.S. Patent 5,840,484 (1998); Rine et al, U.S. Patent No. 5,777,888 (1998); WO 97/27317; WO 99/05323; WO 99/09218; and WO 99/14369. For a device for implementing such techniques, see Lipshutz et al , U.S. Patent No. 5,856,174 (1999) and Anderson et al., U.S. Patent No. 5,922,591 (1999).

In one embodiment, a subset of the inventive DNAs will be arrayed on a substrate, like a gene chip, a filter or a 96-well plate. Test samples containing cells are maintained in the presence of a label capable of incorporation into nascent mRNA. Samples are treated with test and control compounds, which will induce mRNA expression in the sample, resulting in incorporation of label. Whole mRNA is isolated and applied to the array such that it hybridizes with the DNAs contained therein. After washing, the amount of hybridization is quantified and a profile is generated. These steps are repeated with various control and test compounds, thereby generating a library of profiles, which can be used to ascertain the relationships relevant to pharmacological efficacy or toxicity. The matrices used in such profiling, however, need not be limited to those utilizing DNAs. Rather, other nucleic acids, like RNAs and protein nucleic acids (PNAs), as well as the inventive proteins and antibodies corresponding to the inventive proteins may also be employed. Hence, for example, antibodies could form the array and the samples could be treated in order to label nascent proteins. Whole proteins then would be isolated and applied to the antibody matrix. Developing the resulting signal would result in a protein expression profile, which is useful in essentially the same manner as the nucleic acid profile. A protein matrix could be used, for example, in evaluating antibody responses to pharmaceutical agents in order to eliminate possible cross-reactivity.

Moreover, where nucleic acids are used in the matrix, it is often beneficial to use variants (as defined below) of the molecules described herein. This can be used to account for genetic variations that are of little or no consequence to the function of the resultant gene product. Hence, they can account for wobble or conservative amino acid variations that do not perturb function, like variations in some of the protein motifs elucidated below. Thus, each position in the matrix can employ multiple nucleic acid probes that account for a series of variants.

Expression profiling may also be done, in another embodiment, using two- dimensional protein gels in which the inventive proteins are detected. The resultant profiles can be used in the same way as described.

Matrices useful for profiling may be constructed based on different criteria. Of course, the more relevant profiles will take into account expression of most human genes, preferably all of them. In certain situations, however, it is advantageous to look at a smaller subset. For example, if one were concerned about fetal neural toxicity, a fetal brain-specific matrix might be chosen. On the other hand, if one were interested in targeting mammary carcinoma tissue, a corresponding matrix could be used. Thus, matrices may be constructed using all of the sequences available from a tissue-specific library.

* * *

The following discussion relates to some of the various functional and structural groupings that would be of interest to the artisan wishing to construct profiling matrices. Of course, the artisan will also recognized that these functional descriptions may find additional applicability in the therapeutic and diagnostic applications discussed below. Cell Cycle

A proliferating cell must coordinate replication and chromosomal separation to ensure that the genome is replicated completely, and that a single copy, is correctly inherited by each daughter cell. The cell cycle is the coordinated series of events that achieves these aims. Many of the key events are initiated by a family of conserved Seiren/threonine protein kinases, the cyclin-dependent kinases (CDKs), that are activated by the cyclin family of proteins (cyclins A-H). In turn, the cyclin-CDK complexes are modulated by other protein kinases or phosphatases, and by binding specific inhibitor proteins. The enormous variety of ways in which CDK activity can be regulated allows the cell to respond to internal signals generated by preceding events in the cell cycle and to external growth signals.

The somatic cell cycle is divided into four phases: DNA replication (S phase) and chromosome separation (M phase) are separated by gap phases (GI and G2). At specific control points the decision to begin the next stage (DNA synthesis or mitosis) is carefully regulated.

Cdc2, the primary kinase, is especially required for the Gl-S transition and S phase. Cdc4 and Cdc6 are involved at the restriction point, where the cell can decide to proliferate or arrest (GK->G0) and Cdc7 is a CDK activating kinase (CAK) as well as a subunit of TFIIH.

The Cyclin-CDK complexes are regulated in various ways. One is through phosphorylation by CDK activating kinases (CAK), like the Y15 kinase (Weel) and dephosphorylation by CDK associated phosphatases (CAP), like Cdc25A a member of the Cdc25 family (Cdc25A, B and C).

An other way of regulation occurs through two classes of CDK inhibitors (CKI), the INK4 proteins pi 5, pi 6, pi 8, and pi 9, who negatively regulates the cyclin D CDK complexes and second the p21 family with p21, p27, and p57.

The cell cycle is also regulated through ubiquitin-mediated proteolysis involving the destruction of both cyclins and CDK inhibitors by the 26S proteasome, that requires an ubiquitin conjugating enzyme (UBC) and an ubiquitin ligase. The instability is conferred by PEST regions (cyclin D and E) or a ten amino acid region in the amino terminus (degradation box) in the A- and B-type cyclins. All these modifications play an important role for the cellular localization, because only the nuclear CDK-cyclin complexes are functional for cell cycle. During GI phase of the cell cycle, cyclines A, E and D are synthesized and bind to their cyclin-dependent kinase (CDK) partners. CDK complexes containing cyclins A, E and Dl are then imported into and concentrated within nuclei. Cdk6- cyclin D3 has been localized to both cytoplasmic and nuclear compartments, although only the nuclear complex is active. As cells enter S phase, cyclin A and cyclin E complexes remain within the nucleus, whereas cyclin Dl relocalizes to the cytoplasm for proteolysis at the onset of S phase. Like Cdk2-cyclin A, Cdc2-cyclin A is nuclear and remains so until it is degraded during mitosis. By contrast, as a result of ongoing nuclear import and more rapid re-export, cyclin Bl, which binds to Cdc2 upon synthesis during S phase, is predominantly cytoplasmic. Cdc2-cyclin B2 is also cytoplasmic, although this might occur through anchoring of the complex to some cytoplasmic constituent. At prophase, phosphorylation of cyclin Bl promotes accumulation of Cdc2 -cyclin Bl in the nucleus, whereas cyclin B2 remains in the cytoplasm until nuclear envelope breakdown.

Two crucial regulators of Cdc2-cyclin B-Weel and Cdc25C exist and are responsible for the G2 to M control point. Weel is a nuclear protein throughout the cell cycle, whereas Cdc25C binds to 14-3-3 proteins during interphase and remains predominantly cytoplasmic. In some systems Cdc25C, like cyclin Bl, rushes precipitously into the nucleus just before entry into mitosis.

The 110-kDa retinoblastoma (tumor suppressor) protein (RB), a pRB-family member is an important regulator of cell-cycle progression and differentiation. Like the E2F family (E2F1-5) or DP family (DP 1-3) of transcription activators, RB suppresses inappropriate proliferation by arresting cells in GI by repressing the transcription of genes required for the transition into S phase. Before the cell proceeds into S phase, RB becomes phosphorylated at multiple sites by the cyclin dependent protein kinases (CDKs) and loses its transcriptional repressing activity. Phosphorylation of RB during late GI phase results in the dissociation of the E2F-RB repressor complex which allows S-phase specific genes to be transcribed. Cyclin E is the evolutionary conserved target for E2F and interacts together with CDC2 in late GI .

For a proliferating cell it is vital that only undamaged DNA is replicated because if DNA damage is substantial, its replication can lead to chromosome loss or rearrangement. Thus, we find a G1<->S checkpoint in late GI that requires tumor suppressor p53. A p53- dependent GI arrest is effected by the cyclin dependent kinase inhibitor p21 through higher expression levels that inhibits almost all cyclin CDK complexes.

The kinase responsible for phosphorylating the unidentified kinetochore component in metaphase may be a member of the MAP kinase family and appears to be the proto oncogene c-MOS, a cytostatic factor (CSF) in meiosis.

Several categories of proteins are coded for by clones of the invention within the overall group of "Cell cycle"and include, among others, the following:

Tumor suppressors (e.g. N33): Tumour-suppressor genes are known to be involved in the control of cell growth and division, interacting with proteins which control the cell cycle. The N33 gene is significantly methylated in tumour cells, a mechanism by which tumor- suppressor genes are inactivated in cancer. The N33 gene has been reported by OMIN OMIN (Online Mendelian Inheritance in Man at http://www.ncbi.nlm.nih.gov/htbin-post/Omin) to be associated (as potentially diagnostic, therapeutic, causative, and or related, etc..) with the following diseases: 1) prostate cancer suppression (OMIN *601385). Clones in this category include: fbr2_2kl4.

C-TAK1 Cdc25c associated protein kinase: Cdc25C is a protein kinase that controls entry into mitosis by dephosphorylation of Cdc2. Cdc25C function is regulated by phosphorylation, too. Serine 216 phosphorylation of Cdc25C mediates the binding of 14-3-3 protein to Cdc25C. C-TAK1 (Cdc twenty-five C associated protein kinase) phosphorylates Cdc25C on serine 216 in vitro. Alterations in the gene coding for the above protein kinase has been reported by OMIN to be associated (as potentially diagnostic, therapeutic, causative, and or related, etc...) with Pancreatic cancer (OMIN *60278). Clones in this category include: tes3_7j3.

Cell structure and motility

One of the major differences between prokaryotes and eukaryotes is the ability of the eukaryotic cell to adopt very different shapes dependent on its function during the differentiation process. Animal cells vary from being round to extended cylindric forms like motorneurons or muscle cells. In humans, more than 100 different cell types can be distinguished, each having a characteristic shape. The form of a cell often is closely related to its capacity to move. Some completely differentiated cells like fibroblasts can still change their form actively, thereby migrating. Other cell types serve as motor elements - "macroscopically" like muscle cells or "microscopically" like ciliated epithelia. Such tasks are fulfilled by a big class of proteins; on the one hand responsible for maintenance of cell structure and contacting neighbor cells or the intercellular matrix and on the other hand for cell motility. These topics cannot be regarded separately: The motility apparatus e.g. must be fixed in the cytoskeleton. Three different types of filaments can be distinguished: Actin filaments, tubulin filaments and intermediate filaments, each present in almost all types of cells.

Actin filaments (F-actin) are built up of monomers (G- Actin). In muscle cells, actin, myosin, for both of which several paralogous genes are known, as well as many more proteins are constituents of the contractile apparatus.

The "thin" and "thick filaments" in a muscle cell consist mainly of actin and myosin, respectively.

Several different proteins are responsible for the anchoring of the actin filaments in the Z-disks (e.g. alpha-actinin and desmin) or at the end of the myofibers in the cell membrane.

Troponin I, -C, -T and Tropomyosin - associated with actin - confer the Ca++- dependent triggering of contraction.

Length of the sarcomere is controlled by the giant protein titin.

In smooth muscle, there is no troponin. Contraction activity is controlled by phosphorylation / dephosphorylation of myosin by a specialized kinase instead. Contractile fibers are not organized in sarcomeres.

Apart from contributing to muscle contraction, the actomyosin system is responsible for many other motions at cellular level, e.g. the amoeboid movement of pseudopodia or the fission of cells at the end of mitosis by a contractile ring.

Besides this, actin fibers fulfill structural tasks like maintenance of the shape of stereocilia or microvilli. Here, actin filaments are connected by proteins like fimbrin. But not only specialized structures like the mentioned ones contain actin fibers. There is a network covering the complete cell volume with F-actin as a major constituent. Whereas the actin filaments in the structures mentioned above are relatively stable, this F-actin is highly dynamic. Management of the network structure and turnover is achieved by connecting proteins like alpha-actinin, fimbrin or fill-in; turnover is regulated by gelsolin, villin, and different capping- and fragmentation-proteins.

Microtubules are built up of alpha-beta tubulin heterodimers. Turnover of filaments is achieved by building-in and releasing of monomers with different time constant rates at both ends. The resulting cycle is called "treadmilling". Thirteen strings of tubulin duplets build up one subfiber, whereas one fiber contains two or three of those. A complete axoneme consists of 9 radial and 2 central fibers. This "9+2" - structure is the basis both of flagella, their basal bodies and centrioles. In flagella, several additional structures like radial elements exist. Nexin connects the fibers and dyneine is the motor ATPase which shifts the fibers relative to each other. Several genetic diseases like the Cartageneric syndrome are caused by deficiencies of distinct proteins in cilia.

Besides this, microtubules are abundant in all types of cells. They are part of a delivery system for organelles, e.g. in the golgi apparatus. A further very important system based on microtubules is the mitotic spindle, it is organized by the centrosomes. Besides many other components, the major part of a centrosome are two centrioles which are built up of nine microtubule-triplets. Most remarkably, new centrioles are not synthesized de novo but generated by duplication of old ones.

Cytoplasmic microtubules are associated with many different proteins. Two major classes are known: The MAPs ("microtubule-associated proteins", with molecular masses between 200 and 300 kD) and the much smaller tau-Proteins with a MW between 60 and 70 kD. These proteins regulate the treadmill-process and the interaction with other structures in the cell.

Besides actin and myosin the so-called intermediate filaments constitute a third class of filaments. In contrast to the former two groups, they do not participate in motility, nor are they dynamic structures subject to a vivid turnover. The most important ones are neurofilaments (in neurons), keratin filaments (mainly in epithelial cells), and vimentin filaments (in many sorts different cell types).

The biological function of both the cytoskeleton as well as contractile apparatus of a cell does not end at the cell membrane. Cells must be embedded in the extracellular matrix, all cells of a muscle must act as one single mechanical unit and epithelia must resist macroscopic mechanical forces. Hence, cell adhesion and the extracellular matrix are closely connected to the cytoskeleton. Vincullin is one of the proteins which serve as an anchor for intracellular fibers (actin). Different types of desmosomes and tight junctions connect neighbor cells with intercellular fibers. On the inside, cytoplasmic plaques connect them to the cytoskeleton. These structures, on the one hand, serve as mechanical elements whereas gap junctions, on the other hand, connect cells metabolically.

The extracellular matrix consists of a network of proteins, glycoproteins and polysaccharides. Different proteins are present in relation to different mechanical demands:. Elastin is found in tissues with high elasticity (lungs, heart) whereas collagen, a more hard- wearing protein, is found in tendons and ligaments. Fibronectin is an extracellular protein highly important for cell adhesion.

Reference: Murray J et al (1992): Cell Motil Cytoskeleton 22: 211-223.

Within the overall group of Cell Structure and Motility several categories of proteins are coded for by clones of the invention:

Collagen alpha chain proteins: Proteins with the typical (xxG)n repeat of collagen proteins and Pfam von Willebrand factor type A domain(s) suggest they are collagen alpha chains. These proteins can find application in modulation of connective tissue, bone and cartilage development and maintainance. OMIN reports collagen alpha chains have associations (as potentially diagnostic, therapeutic, causative, and/or related, etc..) with the following diseases: 1) Osteogenesis imperfecta, type I (OMIN #166200); 2) Osteogenesis imperfecta congenita (OMIN #166210); 3) Alport Syndrome, X-linked (OMIN #301050); 4) Thrombastenia of Glanzmann and Naegeli (OMIN *273800); 5) Ehlers-Danlos Syndrome, Type VII (OMIN #130060); 6) Marfan Syndrome (OMIN #154700); 7) Alport Syndrome, Autosomal Recessive (OMIN #203780); 8) Alpha-2-Deficient Collagen Disease (OMIN 203760); 9) Goodpasture Syndrome (Omin 233450); 10) Osteogenesis Imperfecta, progressively deforming, with normal sclerae (OMIN #259420); 11) ) Ehlers-Danlos Syndrome, Type VII Autosomal Recessive (OMIN *225410); and 12) ) Osteogenesis imperfecta, Type IV (OMIN #166220). OMIN reports that von Willebrand factor type A domains have associations (as potentially diagnostic, therapeutic, causative, and/or related, etc..) with the following diseases:: 1) Hemophilia A (OMIN *306700); 2) Von Willebrand Disease (OMIN * 193400); 3) Giant Platelet Syndrome (OMIN *231200); 4) Thrombastenia of Glanzmann and Naegeli (OMIN *273800); 5) Congenital Thrombotic Diseasae due to protein C deficiency (OMIN #176860); 6) Polycystic Kidney Disease 1 (OMIN *601313); 7) Nephrogenic Diabetes Insipidus (OMIN *304800); 8) Factor V Deficiency (OMIN *227400); and 9) Dentatorubral-Pallidoluysian Atrophy (Omin * 125370). Clones in this category include: fbr2_2b5.

Radial spokehead protein: Radial spokehead proteins, e.g., Chlamydomonas reinhardtii radial spokehead protein of flagella or axoneme and the Strongylocentrotus purpuratus sea urchin spermatozoa protein p63, and human proteins with similarity thereto are important for the maintenance of a planar form of sperm flagellar beating. The human protein(s) can find application in modulating the structure of the human spermatozoa radial spoke head and modulation of sperm motility in men (e.g., in sterility). Clones in this category include: tes3_15i5.

Ankyrins: Ankyrins are peripheral membrane proteins which interconnect integral proteins with the spectrin-based membrane skeleton. Thus these proteins are involved in coupling of cyto skeleton and cell membrane. OMIN reports that Ankyrins have associations (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with the following diseases: 1) Heriditary Spherocytosis (OMIN * 182900); 2) Hemolytic Poikilocytic Anemia due to reduced ankyrin binding sites (OMIN 141700); 3) Atypical Elliptocytosis (OMIN 225450); 4) Autosomal recessive spherocystosis (OMIN #270970); 5) Werner Syndrome (OMIN *277700); and 6) Rhesus-unlinked type Elliptocytosis (OMIN #130600). Clones in this category include: tes3_l 817.

FGD1 -related F-actin binding protein (Farbin/FGDl): FGD1 -related F-actin-binding protein (Farbin/FGDl) is a novel F-actin-binding protein. The gene locus fgdl seems to be responsible for faciogenital dysplasia or Aarskog-Scott syndrome. (OMIN 305400). Frabin binds F-actin and shows F-actin-cross-linking activity. Overexpression of frabin in Swiss 3T3 cells and COS7 cells induces cell shape change and c-Jun N-terminal kinase activation, as described for FGD1. Because FGD1 has been shown to serve as a GDP/GTP exchange protein for Cdc42 small G protein, it is likely that frabin is a direct linker between Cdc42 and the actin cytoskeleton. Cdc42p is an esin yeast, Cdc42p transduces signals to the actin cytoskeleton to initiate and maintain polarized growth and to mitogen-activated protein morphogenesis. In mammalian cells, Cdc42p regulates a variety of actin-dependent events and induces the JNK/SAPK protein kinase cascade, which leads to the activation of transcription factors within the nucleus. Clones in this category include: tes3_72kl5.

Paramyosins: Paramyosin is a major structural component of thick filaments and invertebrate muscle. Paramyosins are promising antigens for immunization against several parasites, such as Schistosoma mansoni. Clones in this category include: tes3_7b22.

Tuftelin: Tuftelin/enamelin are matrix proteins of the teeth. As other proteins involved in calcification, these proteins are also expressed in the uterus matrix. The new protein can find application in modulation of tissue-calcification, especially the uterus. As reported by OMIN, tuftelin has been associated (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with amelogenesis imperfecta (OMIN *600087). Clones in this category include: utel_19g22.

Cell Adhesion Regulator (CARD: CAR1 is involved in the regulation of cell-cell adhesion. OMIN reports the association (as potentially diagnostic, therapeutic, causative, and/or related, etc...) of CAR1 with tumor suppression by the reduction of tumor invasion (OMIN *116935). Clones in this category include: utel_24j6.

Differentiation Development

Almost every multicellular organism originates from meiotic cell divisions and the recombination of a paternal and a maternal set of chromosomes. After fertilization of the egg, all cells of a body originate from this one cell. Thus the cells of the developing body are initially genetically alike. But phenotypically they become very different. They are specialized to a certain cell type and arranged in an organized pattern to a certain type of tissue and the whole structure has the well-defined shape of an organ. All these features are determined by the DNA sequence of the genome, which is reproduced in every cell. Each cell acts on the genetic instructions given to a certain time and at a certain place of development and plays its individual part in the multicellular organism. Cell differentiation may be divided into three general steps: cell cycle exit, apoptosis protection and tissue specific gene expression. These processes are coordinated to provide the final and unique tissue characteristics.

An animal cell that has achieved a certain level of development is said to be determined. This differentiation of a cell may be irreversible and in that case the cell may be renewed only by simple duplication. Other cells are renewed by means of stem cells which are immortal ( e.g. stem cells of the bone marrow, epidermal stem cells). The genetic control of development is extensively studied in non- vertebrates and vertebrates. The classical animal model is the fruit fly Drosophilia and the modern model is the transgenic mouse. Animal transgenesis has proven to be useful for physiological as well as physiopathological studies. Besides the approach based on the random integration of a DNA construct in the mouse genome, gene targeting can be achieved using totipotent embryonic stem cells for targeted transgenesis. Transgenic mice are than derived from the embryonic stem cells. This allows the introduction of null mutations in the genome (so-called knock-out) or the control of the transgene expression by the endogeneous regulatory sequence of the gene of interest (so- called knock-in). Mice can be created that express wild-type genes, mutant genes, marker genes or cell lethal genes in a tissue specific manner. These animal models allow to follow changes in tissue and organ development and lead to a better understanding of the cellular function of many genes or to the generation of animal models for human diseases. Fundamental problems in immunology, onset and development of cancer, regulation in fatty acid metabolism, aspects of cardiovascular function, control of the central nervous system development, analysis of reproductive development and function are only some examples of research interests.

The final stage of cell differentiation is growth arrest. In animal tissues with rapid cell turnover terminally differentiated cells undergo programmed cell death. The cells have the ability to kill themselves by activating an intrinsic cell suicide program when they are no longer needed or have become seriously damaged. The execution of this program is termed apoptosis. Apoptosis is of importance for development and homeostasis of animals. The key components of this program have been conserved in evolution from worms (C. elegans) to insects (Drosophilia) to humans. The roles of apoptosis include the sculpting of structures during development, deletion of unneeded cells and tissues, regulation of growth and cell number, and the elimination of abnormal and potentially dangerous cells. In this way apoptosis provides "quality control mechanism" that limits the accumulation of harmful cells, such as virus-infected cells and tumor cells. On the other hand inappropriate apoptosis is associated with a wide variety of diseases, including AIDS, neuro-degenerative disorders and ischemic stroke. Because it is now clear that apoptosis is a result of an active, gene-directed process, it should be eventually possible to manipulate this form of cell death by developing drugs that interact with its recently identified mechanisms of action. Inducers of cell differentiation, cell cycle arrest and apoptosis might be the novel molecular targets for new anticancer agents in addition to the signaling pathways for growth factors and cytokines.

Proteins, factors, receptors and genes of importance in apoptosis:

Proteases:

- Calpain, an intracellular cysteine protease, exact role unknown.

- Caspase-1 to Caspase-11, a family of proteases synthesized as an inactive proenzyme. Targets of the activated enzymes include: poly(ADP-ribose) polymerase, DNA- dependent protein kinase, Ul ribonucleoprotein, nuclear laminins and cytoskeleton components (actin).

- Granzyme B, a serine protease released by cytotoxic T-cells.

Receptors:

- CD 95 (synonyms: Fas, APO-1), a receptor protein of the TNF -receptor family which includes TNF-R1 and TNF-R2 with the common characteristic of a 70 amino acid cytoplasmic domain.

- FADD (synonym: MORT-1), a cytoplasmic protein

- DR-3 (synonym: APO-3) a member of the TNF -receptor-family

- DR-4 and DR-5

Genes: - ced-3, ced-4 and ced-9 encode the general apoptotic and antiapoptotic program in Caenorhabditis elegans. Apaf-3 is the mammalian homologue of ced-3.

- Bcl-2 / Bcl-xL / Bax / Bcl-xS / Bak: a large gene family that can either inhibit or promote apoptosis.

- Cytokine response modifier A, a cowpox virus gene whose gene product inhibits caspases.

Others:

- Caspase-activated DNase (CAD) and its inhibitor (ICAD), causes DNA fragmentation in the nucleus

- Ceramide, a complex lipid that acts as a second messenger.

- c-Jun N-terminal kinase (JNK) is a proline-directed kinase

- p53 protein, is essential for the induction of apoptosis as a response to chromosomal damage.

- RAIDD, a death signal-transducing protein.

- Receptor interacting protein (RIP) is an accessory protein with a death domain and a serine/threonine kinase activity.

- Sphingomyelinase, an enzyme that hydrolyzes the complex lipid sphingomyelin to ceramide.

- Tumor necrosis factor (TNF) is a type -II membrane protein

- TNF-receptor associated factor (TRAF2), is an accessory protein that can bind to both TNF-R1 and TNF-R2.

Within the overall group of Differentiation/Development, several categories of proteins are coded for by clones of the invention:

Interleukins (e.g. Interleukin-7): Interleukin precursors related to interleukin-7, for example, are expected to act as new growth factors for human B lineage cells. Additionally, these proteins should induce the gene rearrangement of the T-cell receptor repertoire, leading to thymocyte commitment, and subsequently induce both cytotoxic T-cell- and lymphocyte- activated killer cells These interleukins could find clinical application in a variety of conditions of hematolymphopoietic failure and different tumours, because of its recruitment of B cell lineage cells, cytotoxic T-cell- and lymphocyte-activated killer cells. (OMIN * 146660). Clones in this category include: tes3_35e21.

Testis-specific Y-encoded proteins: The TSPY genes are arranged in clusters on the Y chromosome of many mammalian species. TSPY is believed to function in early spermatogenesis and is a candidate for GBY, the putative gonadoblastoma-inducing gene on the Y. Proteins of the TSPY-SET-NAP1L1 family represent proteins closely related to TSPY. These proteins seem to be involved in early spermatogenesis. Clones in this category include: fbr2_2dl5.

Intracellular transport and trafficking

Eukaryotic cells rely for their viability on the partitioning of many basic cellular processes into membrane-bounded organelles. These are the nucleus, endoplasmic reticulum (ER), Golgi apparatus, endosomes, lysosomal compartments, mitochondria and peroxisomes. Most molecules destined for the lysosome, cell surface and outside the cell are routed through the ER and Golgi, which together with the vesicular intermediates between them, comprise the secretory pathway (Palade 1975). In the ER and Golgi compartments proteins are sorted, modified and often assembled into complexes en route to their final destination. Incorrectly assembled proteins are retained in the ER until they fold correctly or are targeted for degradation. Additional proteins are translocated into and function within the lumenal spaces of organelles or are secreted. Thus a large proportion of proteins synthesized require targeting to membranes either for insertion into or transport across them. A major purpose of this is growth. The secretory pathway is dependent on an intact cytoskeleton and also closely linked to general metabolism by affecting ribosome biogenesis (Mizuta and Warner, 1994). A huge number of proteins is required for targeting, translocation and sorting of newly synthesized proteins.

The first step in sorting is the recognition of cis-acting targeting or signal sequences that organelle-targeted proteins contain. This is carried out by cytosolic targeting factors and/or receptors on the membrane to which the protein is targeted. In some cases the primary sequences are extremely degenerate, with only the overall character being conserved (hydrophobicity for an ER signal sequence, helical amphiphilicity for mitochondrial targeting sequence (Kaiser et al, 1987; Lemire et al, 1989). Following the targeting step, proteins are either inserted into or transported across the membrane (translocated) through a proteinaceous apparatus (termed the translocon). The translocon include or recruit motors to drive the translocation process in the correct direction (Schatz and Dobberstein, 1996). Defined intracellular protein transport steps:

• ER

- targeting to the ER

- translocation into the lumen of the ER, and, depending on the presence of certain signals in the peptide sequence transport through the golgi complex

• Mitochondria

- targeting

- translocation

• Peroxisomes

• The general secretory pathway

- protein modification, assembly and quality control in the ER

- vesicle-mediated trafficking

- vesicle docking and fusion

- transport through the golgi apparatus and sorting at the trans-golgi

- transport to the cell surface

- transport routes to the lysosome

• Endocytosis

• Specialized protein transport routes

• Protein export from the cytoplasm

References: Palade, G (1975) Science 189:347-358; Mizuta et al. (1994) Mol Cell Biol 14: 2493-2502; Kaiser et al. (1987) Science 235: 312-317; Lemire et al. (1989) J Biol Chem 264: 20206-20215; Schatz et al. (1996) Science 271 : 1519-1526.

Rab proteins

In eukaryotic cells the compartmentalisation of processes is a prerequisite for a tight regulation of processes and activities. The cells contain a highly dynamic set of membrane compartments that are responsible for packaging, sorting, secreting, and recycling proteins and other molecules. Trafficking between organelles within the secretory pathway occurs as vesicles derived from a donor compartment fuse with specific acceptor membranes, resulting in the directional transfer of cargo molecules. This process is tightly controlled by the Rab/Ypt family of proteins (reviewed by Novick and Zerial, 1997 ), a branch of the superfamily of small GTPases. Rab proteins regulate a variety of functions, including vesicle translocation and docking at specific fusion sites. Rabs may also play critical roles in higher order processes such as modulating the levels of neurotransmitter release in neurons, a likely mechanism in synaptic plasticity that underlies learning and memory (Geppert and Sudhof, 1998).

Small GTPases share a common three-dimensional fold that, in the GTP bound state, can bind a variety of downstream effector proteins. GTP hydrolysis leads to a conformational change in the "switch" regions that renders the GTPase unrecognizable to its effectors. In this way, by localizing and activating a select set of effectors, a common structural motif is used to control a wide array of distinct cellular processes.

The final steps in membrane fusion are likely to be driven by a set of proteins known as SNAREs. After a vesicle becomes docked, the cytoplasmic domains of VAMP (also termed synaptobrevin) and syntaxin on opposing membranes, in combination with a SNAP- 25 molecule, coalesce into an elongated -helical bundle (Poirier et al., 1998 ; Sutton et al., 1998 ), which may lead to fusion. Because numerous SNARE isoforms have been identified that localize to distinct membrane compartments, it was originally proposed that the specificity of interaction between the SNARE proteins accounted for the specificity in membrane trafficking. Recent results, however, suggest that SNAREs are not specific in their ability to form complexes in vitro, suggesting that trafficking specificity requires additional factors (Yang et al., 1999 ). In this regard, Rab proteins are strong candidates for governing the specificity of vesicle trafficking. Like the SNAREs, many isoforms (40) of the Rab family have been identified that localize to specific membrane compartments (reviewed by Novick and Zerial, 1997 ).

Concomitant with the SNARE cycle, Rab proteins undergo a intricate cycle of membrane and protein interactions. Rabs are posttranslationally modified at C-terminal cysteines by the addition of two geranylgeranyl groups, which mediate membrane association when the Rab is in the GTP-bound state. After guanine nucleotide hydrolysis occurs, the Rab is extracted from the membrane upon forming a complex with a cytosolic GDP-dissociation inhibitor (GDI). This cytosolic intermediate is then recycled onto a newly forming vesicle, most likely through a secondary factor termed a GDI dissociation factor (GDF), which displaces GDI. After the Rab becomes membrane bound, a guanidine nucleotide exchange factor (GEF) promotes release of GDP and the subsequent loading of GTP. In its GTP-bound conformation, the Rab is then free to associate with its specific set of effectors, which can in turn trigger events leading to the eventual fusion of the vesicle with a target membrane. To complete the cycle, perhaps after or concurrent with membrane fusion, a GTPase activating protein (GAP) accelerates nucleotide hydrolysis, switching off the GTPase. The remaining GDP-bound Rab can then participate in a new round of fusion.

Rab interactions with effectors are likely to regulate vesicle targeting and membrane fusion in three ways. First, a Rab may specifically facilitate vectorial vesicle transport. Vesicles are transported from their site of origin to acceptor compartments likely through associations with cytoskeletal elements and transport motors. A protein has been identified with a domain structure that suggests a connection between the cytoskeleton and the Rabs. This protein, called Rabkinesin-6, contains a kinesin-like ATPase motor domain followed by a coiled-coil stalk region and a RBD that specifically binds Rab6 (Echard et al., 1998 ). An additional link with the cytoskeleton is provided by the Rab effector, Rabphilin-3A. Rabphilin-3A has been shown in vitro to interact with -actinin, an actin-bundling protein, but only when not bound to Rab3A (Kato et al., 1996 ). These results raise the intriguing possibility that Rab proteins regulate vesicle interactions with the cytoskeleton and thereby play an active role in targeting vesicles to their appropriate destinations.

Second, Rab proteins may regulate membrane trafficking at the vesicle docking step. A number of Rab effectors, including Rabaptin-5, EEA1, Rabphilin-3A, and Rim, may serve as molecular tethers. Each effector protein contains a RBD, followed by a linker region (some having the potential to form elongated coiled-coil structures), and a domain capable of interacting with a second Rab or the target membrane. Rabaptin-5, for example, contains two RBDs, one near the N terminus that specifically recognizes Rab4 and a second near the C terminus that binds Rab5 (Vitale et al., 1998 ). Both Rim, which is localized to the target membrane, and Rabphilin-3 A, which is localized to the vesicle, contain N-terminal RBDs and C-terminal Ca2+-binding C2 domains, implicating these effectors in synaptic vesicle localization or docking in response to Ca2+ influx (Wang et al., 1997 ). Tethering effectors may also recognize protein complexes on the acceptor membrane. Sec4p, a yeast Rab3A homolog, interacts with the exocyst (Guo et al., 1999 ), a complex of seven or more subunits that is assembled at sites of vesicle fusion along the plasma membrane. The exocyst complex may therefore function as a landmark for Rab/effector-mediated vesicle docking.

Third, once a vesicle has become tethered to its fusion site, Rab proteins may selectively activate the SNARE fusion machinery. The mechanism of this activation is unknown but may involve direct interactions of Rabs or, more likely, their effectors with SNAREs. For example, Hrs-2 is a protein that binds to SNAP-25 and contains a Zn2+-fϊnger motif characteristic of Rab-binding proteins such as Rabphilin-3 A, Rim, EEA1, and Noc2, suggesting that Hrs-2 may form a physical link between Rabs and SNAREs (Bean et al., 1997). In addition, certain mutations in the syntaxin-binding protein Slylp, the Seclp homolog utilized in ER to Golgi trafficking, eliminate the requirement for Yptlp, a Rab protein that functions at this trafficking step (Dascher et al., 1991 ). Rabs may therefore regulate SNARE associations through Seel family members. In support of this idea, a Rab effector was recently found to interact with a vacuole Rab, a Seclp homolog, and a SNARE protein (Peterson et al., 1999 ), which suggests that this effector serves to connect Rab and SNARE function. In this way, Rabs and their effectors may facilitate the correct pairing of SNAREs.

References: Dascher et al. (1991) Mol. Cell. Biol. 11, 872-885; Echard et al. (1998). Science. 279, 580-585; Geppert et al. (1998) Annu. Rev. Neurosci. 21, 75-95; Guo et al. (1999). EMBO J. 18, 1071-1080; Kato et al. (1996) J. Biol. Chem. 271, 31775-31778; Novick et al. (1997) Curr. Opin. Cell Biol. 9, 496-504; Peterson (1999) Curr. Biol. 9, 159- 162; Poirier et al. (1998) Nat. Struct. Biol. 5, 765-769; Vitale et al. (1998) EMBO J. 17, 1941-1951; Wang et al. (1997) Nature. 388, 593-598; Yang et al. (1999) J. Biol. Chem. 274, 5649-5653.

Within the overall group of Intracellular Transport and Trafficking several categories of proteins are coded for by clones of the invention.

Rab proteins:

Rab IB is essential for the intracellular transport of nascent low density lipoprotein (LDL) receptor. It is discussed as a universal mediator of endoplasmatic reticulum to Golgi transport of membrane glycoproteins in mammalian cells. . Clones in this category include: fbr2 2il7, fbr2 3b 16. Rab 10 appear concentrated on membranes in the perinuclear region. Rab 10 has been associated (as potentially diagnostic, therapeutic, causative, and or related, etc..) with the following diseases as reported by OMIN: 1) Choroideremia (OMIN *303199); and 2)RETT Syndrome (OMIN 312750). Clones in this category include: fbr2_62119.

In mice, Rab 17 shows epithelial cell specificity. Rab 17 is discussed as candidate gene for the mouse mutations In (leaden), Tw (twirler), and ax (ataxia). Cloned from a brain cDNA library, the new putative Rab-protein is expected to be involved in vesicle trafficking within neuronal cells. These proteins can find application in modulating the transport of vesicles inside neuronal cells, which are essential for development of functional dendritic processes. . . Clones in this category include: fbr2_41ml5.

Ankyrin G: The ankyrin 3 gene encodes a novel ankyrin, which is expressed in multiple tissues, with very high expression at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Ankyrin G shows several tissue- specific alternative mRNA processing. The different ankyrin G proteins participate in maintenance/targeting of ion channels and cell adhesion molecules to nodes of Ranvier and axonal initial segments. Ankyrin G has been associated (as potentially diagnostic, therapeutic, causative, and/or related, etc ..) with Werner disease (OMIN *277700). Clones in this category include: fkd2_24p5.

Zn-T-transporters : The Zn-T-transporters are membrane proteins that facilitates sequestration of zinc in endosomal vesicles. In the brain, ZnT-3 mRNA seems to be involved in the accumulation of zinc in synaptic vesicles. Zinc (Zn) is an essential element in normal development and metabolism. Recent studies show that in Alzheimer's disease, Zn functions as a double-edged sword, affording protection against Alzheimer's amyloid beta peptide (the major component of senile plaques) at low concentrations and enhancing toxicity at high concentrations by accelerated aggregation of the amyloid beta peptide. These proteins can find application in modulation of Zinc transport in neuronal cells, thus providing means for a modulation of Alzheimer's amyloid beta peptide plaque formation. (OMIN *602878, *602095). Clones in this category include: fbr2_62fl0.

Metabolism

This group includes proteins which are involved in the uptake and consumption of nutrients, and enzymes which are part of the biochemical pathways for energy metabolism or which are involved in the supply of building blocks of nucleic acids, proteins (NTPs, dNTPs, amino acids) for DNA/RNA and protein synthesis, and fatty acids (membranes), to allow for the generation of higher order structures. This group constitutes the most important and largest group in prokaryotes and lower eukaryotes. The higher the evolutionary level of an organism is, however, the more other protein classes like 'signal transduction', 'cell cycle' and 'differentiation and development' increase in importance and number of representatives.

Proteins involved in the metabolism of energy and compounds (here: other than nucleic acids or proteins) are usually the products of house keeping genes, they are often constitutively and/or ubiquitously expressed.

Several categories of proteins are coded for by clones of the invention within the overall group of Metabolism:

NAT1. ARD1 : In yeast, ARD1 and NAT1, are required for the expression of an N- terminal protein acetyltransferase 1. NAT1 controls full repression of the silent mating type locus HML, sporulation and entry into GO. ARD1 is involved in the assembly of the NAT 1- complex. These can find application modulating NAT assembly and action and therefore could be important in metabolism of drugs and environmental mutagens.(OMIN * 108345). Clones in this category include: fbr2_3g8.

Apolipoprotein E receptor: In LDL-receptors the class A domains form the binding site for LDL and calcium. The acidic residues between the fourth and sixth cysteines are important for high-affinity binding of positively charged sequences in LDLR's ligands. These proteins can find application in modulation of cholesterol binding and transport by LDL- receptors and LDL-binding proteins. In normal individuals, chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. In familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants because of a defect in apolipoprotein E. Accumulation of the remnants can result in xanthomatosis and premature coronary and or peripheral vascular disease. OMIN reports that apolipoprotein has associations (as potentially diagnostic, therapeutic, causative, and/or related, etc..) with the following diseases: 1) Familial hypercholesterolemia (OMIN 143890); 2) Familial combined hyperiipidemia (OMIN 144250); and 3) Alzheimer disease. (OMIN #104300). Clones in this category include: fbr2_62017. Ubiquitin carboxyl-terminal hydrolases: Ubiquitin carboxyl-terminal hydrolases (EC 3.1.2.15) (UCH) (deubiquitinating enzymes) are thiol proteases that recognize and hydrolyze the peptide bond at the C-terminal glycine of ubiquitin. These enzymes are involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. OMIN reports that Ubiquitin-specific proteases have associations (as potentially diagnostic, therapeutic, causative, and or related, etc..) with the following diseases: 1) Lung carcinoma (OMIN *603486); 2) x-linked retinal diseases (OMIN *300050); 3) oncogenesis (OMIN *300050);4) ovarian cancer (OMIN *300050). Clones in this category include: fbr2_78k24; htes3_27dl.

Phosphoserine signature (phosphoglucomutases. phosphomannomutase): These proteins take part in the conversion of hexose phosphates. OMIN reports that these proteins have associations (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with the following disease: Fanconi-Bickel Syndrome (OMIN #227810). Clones in this category include: fkd2_24bl5.

NADH ubiquinone oxidoreductase: NADH:ubiquinone oxidoreductase is the first enzyme in the respiratory electron transport chain of mitochondria. It is a a membrane-bound multi-subunit protein. The bovine heart enzyme contains about 40 different polypeptides. OMIN reports that these proteins have associations (as potentially diagnostic, therapeutic, causative, and/or related, etc ..) with the following disease: Brancio-oto-renal syndrome (OMIN *6601445). Clones in this category include: fkd2_3ol7.

Transketolases: Transketolase requires thiamin pyrophosphate as cofactor and shows a wide specificity for both reactants, e.g. converts hydroxypyruvate and R-CHO into CO(2) and R-CHOH-CO-CH(2)OH. OMIN reports that these proteins have associations (as potentially diagnostic, therapeutic, causative, and or related, etc..) with the following diseases: Wernicke-Korsakoff Syndrome (OMIN *277730). Clones in this category include: tes3_17117.

Fatty acid-Co A svnthetases/li gases: These proteins contain AMP-binding domain signature(s), which is present in enzymes which act via an ATP-dependent covalent binding of AMP to their substrate. This domain is found in several CoA synthetases, such as acetate- CoA ligase (EC 6.2.1.1), long-chain-fatty-acid-CoA ligase (EC 6.2.1.3), bile acid-CoA ligase. OMIN reports that these proteins have associations (as potentially diagnostic, therapeutic, causative, and or related, etc..) with the following diseases: 1) Alport syndrome , mental retardation and elliptocytosis (OMIN *300157); 2) Adrenoleukodystrophy (OMIN *300100). Clones in this category include: tes3_35kl7.

ADP/ATP or Adenine Nucleotide Translocataors: These proteins contain mitochondrial energy transfer signature(s) and are most abundant in mitochondria. In its functional state, it is a homodimer of 30-kD subunits embedded asymmetrically in the inner mitochondrial membrane. The dimer forms a gated pore through which ADP is moved from the matrix into the cytoplasm.. OMIN reports that these proteins have associations (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with the following diseases: 1) cardiomyopathy (OMIN * 103220); 2) myopathy (OMIN * 103220); 3)Progressive external ophthalmoplegia (OMIN *601227). Clones in this category include: tes3_35nl2.

Carboxylesterases : OMIN reports that these proteins have associations (as potentially diagnostic, therapeutic, causative, and or related, etc...) with the following diseases: l)hepatic carboxylesterase with detoxification of foreign compounds (OMIN *114835); 2) non-Hodgkin lymphoma (OMIN *114835); 3) B-cell chronic lymphocytic leukemia (OMIN * 114835); 4) rheumatoid arthritis (OMIN * 114835). Clones in this category include: tes3_35n9.

Heat shock proteins: OMIN reports that these proteins have associations (as potentially diagnostic, therapeutic, causative, and or related, etc ..) with the following diseases: 1)27 kd heat shock protein has been correlated with thermotolerance in response to environmental challenges and developmental transitions. (OMIN *6021295). Clones in this category include: utell_23el3.

Nucleic acid management

The genetic information is stored in the form of nucleic acids in all organisms. Two kinds of nucleic acids exist, DNA and RNA. Whereas the more stable DNA in most organisms constitutes the storage form of the genetic information, the labile RNA and in particular mRNA is an intermediate used for the temporal expression of specific genes.

In eukaryotes, DNA is usually a double stranded linear molecule consisting of two antiparallel strands and made up of a deoxyribose, a phosphorus backbone and the four bases A, C, G, and T. The DNA of some organisms has a ring structure. The structure of DNA was unraveled years ago by Watson and Crick. DNA is directional molecule determined by the C- atoms of the sugar.

The most important processes dealing with nucleic acids are:

• replication (e.g. DNA polymerases, Telomerase)

• transcription (RNA polymerases)

• RNA processing (maturation - splicing and degradation)

• in addition, enzymes and proteins exist which require a nucleic acid (mostly RNA) in the active center to be functional (ribozymes - e.g. RNase, Ribosomal proteins)

The DNA of a cell is replicated in the S-phase of the cell cycle. Several enzymes carry out the task of doubling this nucleic acid. As all steps of the cell cycle, also the process of replication is tightly regulated. The enzyme DNA polymerase and several other proteins are involved in this process. Whereas many prokaryotes do have only one origin of replication (i.e., the starting point of the replication cycle), in eukaryotic DNAs (chromosomes) multiple such start points exist. The switch from the synthesis (S) phase to the subsequent G2 or M phases of the cell cycle are dependent on the completion of the replication. This makes clear, that a number of proteins are involved in the replication itself as well as in the control of the process. Since most eukaryotic chromosomes are linear structures, additional proteins and enzymes are necessary to make sure that the structure is maintained through successive generations. This includes those proteins necessary to build the three dimensional structure of chromosomes (e.g. histones) and the structural network of the nucleus and nucleolus (including the defined localization of transcriptionally active genes in the vicinity of nucleoli) but also such enzymes as telomerase which guarantees the integrity of the chromosomal ends.

The expression of genes is usually performed in two steps. First a messenger RNA (mRNA) is produced (transcribed) in one to many copies and second this mRNA is translated into the protein product. The regulation of transcription is discussed under the separate heading 'transcription factors', but also the classes 'signal transduction', 'development', 'cell cycle' and others are affected as the expression of certain genes determines the fate of a cell or organism.

The primary transcript (hnRNA - heterogeneous nuclear RNA) is a single stranded one-to-one copy of the gene as it is located on the chromosome. Before a protein can be translated, already during transcription the process of maturation is initiated. Firstly, a 5' cap structure is enzymatically and covalently added to the RNA, blocking the 5' end of the RNA. Second, when the RNA polymerase has terminated polymerization, the enzyme poly A polymerase adds varying numbers of adenine residues to the 3' end of the transcript. This enzyme recognizes the sequence AAUAAA or AUUAAA (+ some minor variations), cuts the RNA 10 - 30 nucleotides downstream and adds the A residues. The size of the poly A sequence affects the stability of the RNA. Finally, in the process of splicing, the introns present on the genomic level and also present in the hnRNA are spliced out by a multi-protein complex consisting of several proteins and RNAs. The finally maturated mRNA is exported to the cytoplasm where it is translated with help of the ribozymes.

The half life of RNA is usually much shorter than that of DNA. Usually, the mRNA is degraded shortly after synthesis, to guarantee a very defined window of expression of a given gene. This regulation is necessary to specifically maintain or change the set of proteins present at any time in a cell. Specific regions in the 3'UTR (untranslated region) determine the stability of the mRNA in the cytoplasm before it is degraded by RNases, enzymes consisting both of protein and RNA.

References: Watson and Crick (1953) Nature 171: 737-738.

Several categories of proteins are coded for by clones of the invention within the overall group of "Nucleic acid managemenf'and include, among others, the following:

RNA helicases including DEAD/H box helicases: RNA helicases comprise a large family of proteins that are involved in basic biological systems such as nuclear and mitochondrial splicing processes, RNA editing, rRNA processing, translation initiation, nuclear mRNA export, and mRNA degradation. RNA helicases are essential factors in cell development and differentiation, and some of them play a role in transcription and replication of viral single-stranded RNA genomes. The members of the largest subgroup, the DEAD and DEAH box proteins, exhibit a strong dependence of the unwinding activity on ATP hydrolysis. DEAD box proteins have been associated (as potentially diagnostic, therapeutic, causative, and/or related, etc..) as reported by with the following disease processes and/or genes: 1) ataxia-telangiectasia gene: "A human gene (DDX10) encoding a putative DEAD- box RNA helicase at 1 Iq22-q23" Genomics 33:199-206, 1996, Savitsky et al., (OMIN *601235); 2) hematopoetic tumors: "Cloning and expression of a murine cDNA homologous to the human RCK P54, a lymphoma-linked chromosomal breakpoint 1 lq23", Gene 166:293- 6, 1995, Seto et al. (OMIN *600326); 3) dermatomyositis: a) "The major dermatomyositis- specific Mi-2 autoantigen is a presumed helicase involved in transcriptional activation." Arthritis Rheum. 38: 1389-1399, 1995, Seelig et al. (OMIN *603277); b) "Two forms of the major antigenic protein of the dermatomyositis-specific Mi-2 autoantigen." (Letter), Arthritis Rheum. 39: 1769-1771, 1996., Seelig et al. (OMIN *603277); c) "The dermatomyositis- specific autoantigen Mi2 is a component of a complex containing histone deacetylase and nucleosome remodeling activities", Cell 95: 279-289, 1998. Zhang et al. (OMIN *603277); 4) Muscular Dystrophy, Pseudohypertrophic Progressive Duchenne and Becker Types (OMIN *310200); 5) Mucopolysaccharidosis Type IVA (OMIN *253000); 6) Albinism I (OMIN *203100); 7) Wilms Tumor 1 (OMIN * 194070); 8) Spinocerebellar Ataxia 7 (OMIN * 164500). Clones in this category include: fbr2_23bl0, fbr2_3cl8, fbr2_6ol7, fbr2_82i24, and tes3_14h21.

Inorganic pyrophosphatase: Inorganic pyrophosphatase (EC 3.6.1.1) (PPase) is the enzyme responsible for the hydrolysis of pyrophosphate (PPi) which is formed as the product of the many biosynthetic reactions that utilize ATP. All known PPases require the presence of divalent metal cations, with magnesium conferring the highest activity. Clones in this category include: fbr2_64al5.

DNA-damage -inducible protein (dinP) or Proteins induced by DNA-Damage: The dinB/P pathway is a second SOS-pathway in E.coli. Genes related to this seem to be involved in modulating DNA repair and mutagenesis. Clones in this category include: fbr2_72M8_i

Proteins with myc-tvpe, helix-loop-helix dimerization domain signature(s). This helix-loop-helix domain mediates protein dimerization has been found in proteins such as the myc family of cellular oncogenes, proteins involved in myogenesis and vertebrate proteins that bind specific DNA sequences in various immunoglobulin chains enhancers. Therefore, these proteins could be novel DNA-binding proteins. Clones in this category include: fbr2_72112.

Cytosolic ribosomal proteins L36: L36 seems to be part of the eukaryotic ribosomal peptidyl transferase center and can find application in modulation of ribosome assembly, maintenance and activity. Clones in this category include: fkd2_3b2.

Ribonuclease H: Ribonuclease H proteins are RNA modificating proteins and have been associated (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with the following diseases as reported by OMIN: 1) Adenomatous Polyposis of the Colon (OMIN * 175100); 2) Retinoblastoma (OMIN * 180200) ; and 3) Von Hippel-Lindau Syndrome (OMIN * 193300). Clones in this category include: phtes3_15j3.

Signal transduction

Cells in higher order organisms need to continuously communicate with its environment especially with other cells of the same organism in order to maintain the function and specialization of the whole system these cells are part of. This important task of communication is performed with help of cell-surface receptors which receive and transmit signals from outside into the cell. G-proteins

The largest known family of cell-surface receptors is that of the G-protein-coupled receptors, which mediate the transmission of diverse stimuli such as neurotransmitters, glycopeptides, hormones, peptides, odorant molecules, and photons. The functional unit of these receptors is composed of the receptor molecule itself (GPCR) which is anchored in the cytoplasma membrane with seven membrane spanning domains, the heterotrimeric G-protein which is composed of α and βγ-subunits (Gα and Gβγ), and the effectors that interact with Gα and / or Gβγ. In particular, the dissociated Gα and Gβγ can regulate the activities of a number of effector molecules such as adenylate cyclases, phopholipase C isoforms, ion channels, and tyrosine kinases, resulting in a variety of cellular functions. The process of signal transduction must be tightly regulated and reversible in order to avoid overstimulation, to achieve signal termination, and render the receptor responsive to subsequent stimuli [Iacovelly L. et al., (1999) FASEB J. 13, 1-8, Hamm, H.E. (1998) J. Biol. Chem. 273, 669- 672].

G-proteins are GTPases that, upon binding of GTP change their conformation which in return unmasks structural motives, in particular the so called effector loop, which can mediate the interactions to target proteins, or effectors, for the GTPases. This ability enables the GTPases to cycle between active, GTP-bound and inactive, GDP bound conformations and in the process to function as molecular traffic lights in a multitude of signal transduction pathways. The most important of these signal transduction pathways that are regulated with help of G-proteins are that of the phospholipase C / protein kinase C and that of the adenylate cyclase / protein kinase A. The cycling of GTPases is tightly regulated by three main classes of proteins: The exchange of hydrolyzed GDP for a fresh GTP is facilitated by guanosine nucleotide exchange factors (GEFs), the hydrolysis of GTP to GDP is sped up by GTPase-activating proteins (GAPs), and the dissociation of GDP from the GTPases is inhibited by GDP dissociation inhibitors (GDIs) [Tapon and Hall (1997) Curr.Opin. Cell. Biol. 9, 86-92, Van Aelst and D- Souza-Schorey (1997) Genes Dev. 11, 2295-2322].

SOC-familv

A conserved motif that was originally identified in proteins that negatively regulate the signaling action of cytokines was termed SOCS box, the Suppressor Of Cytokine

Signaling. Based on homology, five distinct structural protein classes have been identified since that carry this motif. The function of most of these proteins is presently not known.

Common to the proteins is only the SOCS box which is located near the C-terminus of the respective peptides. Recently, the SOCS box has been demonstrated to induce binding of proteins to elongins B and C which could target the proteins (and bound substrates) to the proteasomal protein degradation pathway (Kamura, T. et al. (1998) Genes Dev. 12, 3872-

3881; Zhang, J.-G. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 2071-2076).

The class where the SOCS box was originally described contains several members (SOCS-l-SOCS-7 and CIS). In addition to the SOCS box, these proteins also contain a SH2 (Src-homology 2) domain and a variable N-terminus. These SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Upon cytokine stimulation, expression of SOCS proteins is rapidly induced and the proteins inhibit further cytokine action. The mode of action of the SOCS proteins is variable. While SOCS-1 binds and inhibits the JAK (Janus kinases) family of cytoplasmic protein kinases [Narahzaki M. et al (1998) Proc. Natl. Acad. Sci. USA 95, 13130-13134, Nicholson, S.E. et al (1999) EMBO. J. 18, 375-385], CIS appears to act by competing with signaling molecules such as the STATs (Transducers and Activators of Transcription) family for binding to phosphorylated receptor cytoplasmic domains [Yoshimura, A. et al. (1995) EMBO J. 14, 2816-2826; Matsumoto, A. et al (1997) BloodS9, 3148-3154].

A second class of SOCS box protein contains additionally WD-40 repeats which were initially identified in the mouse WSB-1 and -2 proteins. The functions of WD-40 proteins are not completely understood but seem to be rather divergent. In Cdc4p the WD-40 repeats probably are necessary for binding the substrate for Cdc34p [Mathias, N. et al. (1999) Mol. Cell Biol. 19, 1759-1767]. Cdc4p is a component of a ubiquitin ligase that tethers the ubiquitin-conjugating enzyme Cdc34p to its substrates. The posttranslational modification of a protein by ubiquitin usually results in rapid degradation of the ubiquitinated protein by the proteasome. The transfer of ubiquitin to substrate is a multistep process where WD-40 repeats might play an important function.

Other WD-40 containing proteins (e.g. the retino blastoma binding protein RbAp48) have been shown to bind metal ions (Zinc) and that this metal binding might mediate and/or regulate protein-protein interactions which are functionally important in chromatin metabolism [Kenzior, A.L. and Folk, W.R. (1998) FEBSLett. 440, 425-429]. These proteins are involved in the RAS-cAMP pathway that regulates cellular growth [Ach R.A. et al. (1997) Plant Cell 9, 1595-1606].

The SPRY domain has been identified in pyrin or marenostrin, a protein which is mutated in patients with Mediterranean fever and which is similar to the butyrophilin family. While butyrophilins seem to be involved in the lactation process in mammals, the function pyrin is unknown. Three proteins (SSB-1 to -3) have been identified to contain both SPRY and SOCS box motifs. The function of these proteins is also not known.

Ankyrin repeat containing proteins share a 33-residue repeating motif, an L-shaped structure with protruding β-hairpin tips which mediate specific macromolecular interactions with cytoskeletal, membrane, and regulatory proteins. These proteins play fundamental roles in diverse biological activities including growth and development, intracellular protein trafficking, the establishment and maintenance of cellular polarity, cell adhesion signal transduction, and mRNA transcription. Three proteins that contain ankyrin repeats (ASB-1 to -3) have been identified to contain a C-terminal SOCS box additionally to the ankyrin repeats. The function of these proteins or the individual domains remains to be discovered [Hilton, D.J. et al (1998) Proc. Natl. Acad. Sci. USA 95, 114-119].

A few small GTPases (RAR and RAR like) do also contain a SOCS box. GTPases are involved in signal transduction during cellular communication. The function of the SOCS box in this type of proteins is currently unclear [Hilton, D.J. et al (1998) Proc. Natl. Acad. Sci. USA 95, 114-119].

Ca ²⁺ as second messenger

The bivalent cation Ca²⁺ is, besides cAMP, one of the two major second messengers in eukaryotic cells. Its intracellular concentration is tightly regulated and usually kept very low compared to the cell's environment. Ca²⁺ binding proteins and transporters (Gap junction, Voltage-gated, second messenger-gated) help to sequester huge amounts of the ion in various organelles from where Ca²⁺ can be released upon extracellular stimuli. E.g. the contraction of the muscle is dependent on the presence of Ca²⁺ ions which are readily transported back into the organelles in order for the muscle to relax. In signal transduction, Ca²⁺ functions as a second messenger that activates Ca²⁺ dependent processes through the activation of Ca²7calmodulin dependent protein kinases (CaM kinases) which are the major effector molecules of Ca²⁺. In the signaling cascades, the CaM dependent kinases activate phospholipases (e.g. phospholipase C) that in return activate other protein kinases such as protein kinase C. cAMP

The cyclic AMP is produced by the enzyme adenylate cyclase in response to extracellular signals. Certain G-proteins stimulate the activity of adenylate cyclase which converts ATP to cAMP and PPi. Two molecules of cAMP bind to each of two regulatory subunits of cAMP dependent protein kinase which in turn dissociate from the two catalytic subunits of the heterotetramer R₂C₂. Upon release of the C-subunits, they become active and phosphorylate substrate proteins at Ser and Thr residues. The process leading from binding of extracellular molecules to their receptors, the transmission of the stimuli into the cell, the activation of adenylate cyclase and the subsequent activation of cAMP dependent protein kinase is one of two major signal transduction pathways in eukaryotic cells. Since the phosphorylation of proteins is a posttranslational modification of proteins, the kinases are described in the class "signal transduction."

SARA

Members of the transforming growth factor β (TGFβ) superfamily signal through a family of cell-surface transmembrane serine/threonine kinases, known as type I and type II receptors (Heldin et al., 1997 ; Attisano and Wrana, 1998 ; Kretzschmar and Massague,

1998). Ligand induces formation of heteromeric complexes of these receptors, and signaling is initiated when receptor I is phosphorylated and activated by the constitutively active kinase of receptor II (Wrana et al., 1994 ). The activated type I receptor kinase then propagates the signal to a family of intracellular signaling mediators known as Smads (contraction of the

C.elegans Sma and Drosophila Mad genes which were the first identified members of this class of signaling effectors). Three classes of Smads with distinct functions have been defined: the receptor- regulated Smads, which include Smadl, 2, 3, 5, and 8; the common mediator Smad, Smad4; and the antagonistic Smads, which include Smad6 and 7 (Heldin et al., 1997; Attisano and Wrana, 1998 ; Kretzschmar and Massague, 1998 ). Receptor-regulated Smads (R-Smads) act as direct substrates of specific type I receptors, and the proteins are phosphorylated on the last two serines at the carboxyl terminus within a highly conserved SSXS motif (Macias-Silva et al., 1996 ; Abdollah et al., 1997 ; Kretzschmar et al., 1997 ; Liu et al., 1997b ; Souchelnytskyi et al., 1997 ). Regulation of R-Smads by the receptor kinase provides an important level of specificity in this system. Thus, Smad2 and Smad3 are substrates of TGFβ or activin receptors and mediate signaling by these ligands (Macias-Silva et al., 1996 ; Liu et al., 1997b ; Nakao et al., 1997 ), whereas Smadl, 5, and 8 are targets of BMP receptors and propagate BMP signals (Hoodless et al., 1996 ; Chen et al., 1997b ; Kretzschmar et al., 1997 ; Nishimura et al., 1998 ). Once phosphorylated, R-Smads associate with the common Smad, Smad4 (Lagna et al., 1996 ; Zhang et al., 1997 ), and mediate nuclear translocation of the heteromeric complex. In the nucleus, Smad complexes then activate specific genes through cooperative interactions with DNA and other DNA-binding proteins such as FASTI, FAST2, and Fos/Jun (Chen et al., 1996 , Chen et al., 1997a ; Liu et al., 1997a ; Labbe et al., 1998 ; Zhang et al., 1998 ; Zhou et al., 1998 ). In contrast to R-Smads and Smad4, the antagonistic Smads, Smadό and 7, appear to function by blocking ligand-dependent signaling (reviewed in Heldin et al., 1997 ).

Phosphorylation of R-Smads by the type I receptor is essential for activating the TGFβ signaling pathway (Heldin et al., 1997 ; Attisano and Wrana, 1998 ; Kretzschmar and Massague, 1998 ). However, little is known of how Smad interaction with receptors is controlled. A novel Smad2/Smad3 interacting protein has been described (Tsukazaki T. et al., 1998 ) that contains a double zinc finger, or FYVE domain, and which has been called SARA (Smad anchor for receptor activation). The SARA motif recruits Smad2 into distinct subcellular domains and co-localizes and interacts with TGFβ receptors. TGFβ signaling induces dissociation of Smad2 from SARA with concomitant formation of Smad2/Smad4 complexes and nuclear translocation. Moreover, deletion of the FYVE domain in SARA causes mislocalization of Smad2 and inhibits TGFβ-dependent transcriptional responses. Thus, SARA defines a component of TGFβ signaling that functions to recruit Smad2 to the receptor by controlling the subcellular localization of Smad. References: Abdollah et al. (1997) J. Biol. Chem. 272, 27678-27685; Attisano et al. (1998) Curr. Opin. Cell Biol. 10, 188-194; Chen et al. (1996) Nature 383, 691-696; Chen et al. (1997a) Nature 389, 85-89; Chen et al. (1997b) Proc. Natl. Acad. Sci. USA 94, 12938-12943; Heldin et al. (1997) Nature 390, 465-471; Hoodless et al. (1996) Cell 85, 489-500; Kretzschmar et al. (1998) Curr. Opin. Genet. Dev. 8, 103-111; Kretzschmar et al.

(1997) Genes Dev. 11, 984-995; Labbe et al. (1998) Mol. Cell 2, 109-120; Lagna et al. (1996) Nature 383, 832-836; Liu et al. (1997a) Genes Dev. 11, 3157-3167; Liu et al. (1997b) Proc. Natl. Acad. Sci. USA 94, 10669-10764; Macias-Silva et al. (1996) Cell 87, 1215-1224; Nakao et al. (1997) EMBO J. 16, 5353-5362; Nishimura et al. (1998) J. Biol. Chem. 273, 1872-1879; Souchelnytskyi et al. (1997) J. Biol. Chem. 272, 28107-28115; Tsukazaki et al. (1998) Cell 95, 779-791; Wrana et al. (1994) Nature 370, 341-347; Zhang et al. (1997) Curr. Biol. 7, 270-276; Zhang et al. (1998) Nature 394, 909-913; Zhou et al.

(1998) Mol. Cell 2, 121-127.

Calcium

The bivalent cation Ca²⁺ is, along with cAMP, one of the two major second messengers in eukaryotic cells. Its intracellular concentration is tightly regulated and usually kept very low compared to the cell's environment. Ca²⁺ binding proteins and transporters

(Gap junction, Voltage-gated, second messenger-gated) help to sequester huge amounts of the ion in various organelles from where Ca²⁺ can be released upon extracellular stimuli. E.g. the contraction of the muscle is dependent on the presence of Ca²⁺ ions which are readily transported back into the organelles in order for the muscle to relax. In signal transduction,

Ca²⁺ functions as a second messenger that activates Ca²⁺ dependent processes through the activation of Ca²7calmodulin dependent protein kinases (CaM kinases) which are the major effector molecules of Ca²⁺. In the signaling cascades, the CaM dependent kinases activate phospholipases (e.g. phospholipase C) that in return activate other protein kinases such as protein kinase C.

Rab proteins

In eukaryotic cells the compartmentalization of processes is a prerequisite for a tight regulation of processes and activities. The cells contain a highly dynamic set of membrane compartments that are responsible for packaging, sorting, secreting, and recycling proteins and other molecules. Trafficking between organelles within the secretory pathway occurs as vesicles derived from a donor compartment fuse with specific acceptor membranes, resulting in the directional transfer of cargo molecules. This process is tightly controlled by the Rab/Ypt family of proteins (reviewed by Novick and Zerial, 1997 ), a branch of the superfamily of small GTPases. Rab proteins regulate a variety of functions, including vesicle translocation and docking at specific fusion sites. Rabs may also play critical roles in higher order processes such as modulating the levels of neurotransmitter release in neurons, a likely mechanism in synaptic plasticity that underlies learning and memory (Geppert and Sudhof, 1998 ).

Small GTPases share a common three-dimensional fold that, in the GTP bound state, can bind a variety of downstream effector proteins. GTP hydrolysis leads to a conformational change in the "switch" regions that renders the GTPase unrecognizable to its effectors. In this way, by localizing and activating a select set of effectors, a common structural motif is used to control a wide array of distinct cellular processes.

The final steps in membrane fusion are likely to be driven by a set of proteins known as SNAREs. After a vesicle becomes docked, the cytoplasmic domains of VAMP (also termed synaptobrevin) and syntaxin on opposing membranes, in combination with a SNAP- 25 molecule, coalesce into an elongated -helical bundle (Poirier et al., 1998 ; Sutton et al., 1998 ), which may lead to fusion. Because numerous SNARE isoforms have been identified that localize to distinct membrane compartments, it was originally proposed that the specificity of interaction between the SNARE proteins accounted for the specificity in membrane trafficking. Recent results, however, suggest that SNAREs are not specific in their ability to form complexes in vitro, suggesting that trafficking specificity requires additional factors (Yang et al., 1999 ). In this regard, Rab proteins are strong candidates for governing the specificity of vesicle trafficking. Like the SNAREs, many isoforms (40) of the Rab family have been identified that localize to specific membrane compartments (reviewed by Novick and Zerial, 1997 ).

Concomitant with the SNARE cycle, Rab proteins undergo a intricate cycle of membrane and protein interactions. Rabs are posttranslationally modified at C-terminal cysteines by the addition of two geranylgeranyl groups, which mediate membrane association when the Rab is in the GTP-bound state. After guanine nucleotide hydrolysis occurs, the Rab is extracted from the membrane upon forming a complex with a cytosolic GDP-dissociation inhibitor (GDI). This cytosolic intermediate is then recycled onto a newly forming vesicle, most likely through a secondary factor termed a GDI dissociation factor (GDF), which displaces GDI. After the Rab becomes membrane bound, a guanidine nucleotide exchange factor (GEF) promotes release of GDP and the subsequent loading of GTP. In its GTP-bound conformation, the Rab is then free to associate with its specific set of effectors, which can in turn trigger events leading to the eventual fusion of the vesicle with a target membrane. To complete the cycle, perhaps after or concurrent with membrane fusion, a GTPase activating protein (GAP) accelerates nucleotide hydrolysis, switching off the GTPase. The remaining GDP-bound Rab can then participate in a new round of fusion.

Rab interactions with effectors are likely to regulate vesicle targeting and membrane fusion in three ways. First, a Rab may specifically facilitate vectorial vesicle transport. Vesicles are transported from their site of origin to acceptor compartments likely through associations with cytoskeletal elements and transport motors. A protein has been identified with a domain structure that suggests a connection between the cytoskeleton and the Rabs. This protein, called Rabkinesin-6, contains a kinesin-like ATPase motor domain followed by a coiled-coil stalk region and a RBD that specifically binds Rab6 (Echard et al., 1998 ). An additional link with the cytoskeleton is provided by the Rab effector, Rabphilin-3A. Rabphilin-3A has been shown in vitro to interact with -actinin, an actin-bundling protein, but only when not bound to Rab3A (Kato et al., 1996 ). These results raise the intriguing possibility that Rab proteins regulate vesicle interactions with the cytoskeleton and thereby play an active role in targeting vesicles to their appropriate destinations.

Second, Rab proteins may regulate membrane trafficking at the vesicle docking step. A number of Rab effectors, including Rabaptin-5, EEA1, Rabphilin-3 A, and Rim, may serve as molecular tethers. Each effector protein contains a RBD, followed by a linker region (some having the potential to form elongated coiled-coil structures), and a domain capable of interacting with a second Rab or the target membrane. Rabaptin-5, for example, contains two RBDs, one near the N terminus that specifically recognizes Rab4 and a second near the C terminus that binds Rab5 (Vitale et al., 1998 ). Both Rim, which is localized to the target membrane, and Rabphilin-3 A, which is localized to the vesicle, contain N-terminal RBDs and C-terminal Ca2+-binding C2 domains, implicating these effectors in synaptic vesicle localization or docking in response to Ca2+ influx (Wang et al., 1997 ). Tethering effectors may also recognize protein complexes on the acceptor membrane. Sec4p, a yeast Rab3A homolog, interacts with the exocyst (Guo et al., 1999 ), a complex of seven or more subunits that is assembled at sites of vesicle fusion along the plasma membrane. The exocyst complex may therefore function as a landmark for Rab/effector-mediated vesicle docking.

Third, once a vesicle has become tethered to its fusion site, Rab proteins may selectively activate the SNARE fusion machinery. The mechanism of this activation is unknown but may involve direct interactions of Rabs or, more likely, their effectors with SNAREs. For example, Hrs-2 is a protein that binds to SNAP-25 and contains a Zn2+-fϊnger motif characteristic of Rab-binding proteins such as Rabphilin-3 A, Rim, EEA1, and Noc2, suggesting that Hrs-2 may form a physical link between Rabs and SNAREs (Bean et al., 1997). In addition, certain mutations in the syntaxin-binding protein Slylp, the Seclp homolog utilized in ER to Golgi trafficking, eliminate the requirement for Yptlp, a Rab protein that functions at this trafficking step (Dascher et al., 1991 ). Rabs may therefore regulate SNARE associations through Seel family members. In support of this idea, a Rab effector was recently found to interact with a vacuole Rab, a Seclp homolog, and a SNARE protein (Peterson et al., 1999 ), which suggests that this effector serves to connect Rab and SNARE function. In this way, Rabs and their effectors may facilitate the correct pairing of SNAREs.

References: Dascher et al. (1991). Mol. Cell. Biol. 11, 872-885; Echard et al. (1998). Science. 279, 580-585; Geppert et al. (1998). Annu. Rev. Neurosci. 21, 75-95; Guoet al. (1999). EMBO J. 18, 1071-1080; Kato et al. (1996). J. Biol. Chem. 271, 31775-31778; Novick et al. (1997). Curr. Opin. Cell Biol. 9, 496-504; Peterson et al. (1999). Curr. Biol. 9, 159-162; Poirier et al. (1998). Nat. Struct. Biol. 5, 765-769; Vitale et al. (1998). EMBO J. 17, 1941-1951; Wang et al. (1997). Nature. 388, 593-598; Yang et al. (1999). J. Biol. Chem. 274, 5649-5653.

Kinases

Reversible posttranslational modifications of proteins are major means of regulating cellular activities. Among the various modifications that are carried out by the cells, the addition of phosphoryl groups to Ser/Thr or Tyr residues is the most important and widely used. The phosphorylation of proteins is accomplished by protein kinases, while the reverse reaction, the removal of phosphoryl groups, is carried out by phosphatases. Kinases /

Phosphatases regulate key positions e.g. in the processes of cell proliferation, differentiation and communication/signaling. These processes must be tightly regulated in order to maintain a steady state level of cellular fate. Mis-regulation of kinase activities (or that of phosphatases) is made responsible for a multitude of disease processes such as oncogenesis, inflammatory processes, arteriosclerosis, and psoriasis.

Protein kinases constitute the largest protein family that is currently known. Several hundred kinases have been identified already. Classically, kinases are subdivided into two classes based on the amino acid residues in their substrates that are phosphorylated by the particular enzymes. The kinases specifically add phosphoryl groups from adenosine triphosphate (ATP) or, less frequently, guanosine triphosphate (GTP), either to serine and/or threonine or to tyrosine residues of substrate proteins. An estimated 1,000 to 10,000 proteins present in a typical mammalian cell are believed to be regulated also by the action of protein kinases.

Protein kinases are frequently integral parts of signaling cascades that transmit extracellular stimuli (e.g. hormones, neurotransmitters, growth- or differentiation factors) into the cell and result in various responses by the cells. The kinases play key roles in these cascades as they constitute a sort of 'molecular switches' turning on or off the activities of other enzymes and proteins, e.g. metabolic, regulatory, channels and pumps, receptors, cytoskeletal, transcription factors.

The regulation of kinase activities is accomplished by various means:

The best characterized example for the regulation via regulatory subunits is the cAMP-dependent protein kinase (PKA) which is also a prototype for second messenger activated protein kinases. This enzyme consists of a heterotetramer of two catalytic (C) and two regulatory (R) subunits. Upon binding of two molecules of second messenger (cAMP) in each R subunit, the catalytic subunits are released and active. Both of the catalytic and the regulatory subunits several isoforms exist. The combination of catalytic and regulatory subunits determines the localization of the holoenzyme and also the substrate spectrum that is available for phosphorylation. The consensus pattern necessary to be present in the substrate for PKA action is RRXS/T where X can be any amino acid.

The casein kinase II comprises another examples for holoenzymes that consist of catalytic and regulatory subunits. Other kinases that are activated by second messengers are cGMP-dependent protein kinase and Protein kinase C (PKC) which is activated by diacylglycerol, which in turn is produced by phospholipases by cleavage of phosphatidylcholine. Receptor kinases usually consists of an extracellular domain which can bind effector molecules (e.g. growth factors and hormones) and transfer the stimulus to the intracellular domain of these proteins which usually is a protein tyrosine kinase. Other tyrosine kinases lack an extracellular domain but are associated with receptors which transfer the signal after effector binding by activating the associated protein kinase enzyme (e.g. Src kinase family; Src, Blk, Fgr, Fyn, Lck Lyn, Yes and Janus kinase family; Jakl-3, Tyk2).

Dysfunction of kinases, e.g. caused by non-functioning regulation, can be the cause of inflammatory diseases and uncontrolled proliferation. v-Src which is a truncated version of the C-Src protooncogene tyrosine kinase is a classical example for this process as v-Src does not contain the regulatory domain of the cellular gene and is thus constitutively active.

Several categories of proteins are coded for by clones of the invention within the overall group of "Signal transduction"and include, among others, the following:

Neurocalcin (Recoverin): Neurocalcin is a Ca(2+)-binding protein with three putative Ca(2+)-binding domains (EF -hands). In cattle, 6 isoforms are differentially expressed in the central nervous system, retina and adrenal gland. Homology with recoverin indicates involvement in Ca2+ dependent activation of guanylate cyclase.. These proteins can find application in modulating/blocking the guanylate cyclase-pathway. Diseases associated (as potentially diagnostic, therapeutic, causative, and/or related, etc..) with these proteins include as reported by OMIN 1) autosomal dominant cone dystrophy (OMIN *600364); 2) cone dystrophy 3 (OMIN *600364); 3) cancer associated retinopathy (OMIN *179618). Clones in this category include: fbr2_23b21.

Proteins with a WW Domain: Proteins that contain a WW domain which has been originally described as a short conserved region in a number of unrelated proteins, among them dystrophin, the gene responsible for Duchenne muscular dystrophy. The domain, which spans about 35 residues, is repeated up to 4 times in some proteins. It has been shown to bind proteins with particular proline-motifs, [AP]-P-P-[AP]-Y, and thus resembles somewhat SH3 domains. This domain is frequently associated with other domains typical for proteins in signal transduction processes. Examples of proteins containing the WW domain are Dystrophin, Utrophin, vertebrate YAP protein (binds the SH3 domain of the Yes oncoprotein), murine NEDD-4 (embryonic development and differentiation of the central nervous system), IQGAP (human GTPase activating protein acting on ras). Therefore these proteins should be involved in intracellular signal transduction. Diseases associated (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with these proteins include as reported by OMIN 1) Muscular Dystrophy, Pseudohypertrophic Progressive Duchenne and Becker Types (OMIN *310200). Clones in this category include: fbr2_23nl6.

Protein substrates for cAMP-dependent protein kinase: Acting as a choride channel or chloride channel inhibitor these proteins have been associated (as potentially diagnostic, therapeutic, causative, and/or related, etc..) as reported by OMIN with Cystic Fibrosis (OMIN #219700). Clones in this category include fbr2_82il7.

Sphingosine kinase: Sphingosine kinase is a new type of lipid kinase, which is regulated by growth factors. The enzyme phosphorylates sphingosine, which subsequently exerts intracellular and extracellular actions. Intracellulary, sphingosine 1 -phosphate (SPP) promotes proliferation and inhibits apoptosis. In yeast, survival of cells exposed to heat shock indicates is dependent on SPP. Extracellulary, SPP inhibits cell motility and influences cell morphology, effects that appear to be mediated by the G protein-coupled receptor EDG1. These proteins have been associated (as potentially diagnostic, therapeutic, causative, and/or related, etc...) as reported by OMIN with Gaucher Disease, Type I (OMIN *230800). Clones in this category include fbr2_82m6.

Vanilloid Receptors: VR1 seems to play an important role in the activation and sensitization of nociceptors. It is the receptor for e.g. capsaicin, a selective activator of nociceptors, a natural product of capsicum peppers. Related can find application as a target for the development of new nociception-modulating drugs. Clones in this category include tes3_20k2.

RCCl (Regulator of chromosome condensation): RCCl (regulator of chromosome condensation) is a eukaryotic protein which binds to chromatin and interacts with ran, a nuclear GTP-binding protein. RCCl promotes the exchange of bound GDP with GTP, acting as a guanine-nucleotide dissociation stimulator. These proteins can find application in the regulation of gene expression by activition of nuclear GTP-binding proteins. The X-linked retinitis pigmentosa is a result of a defect GTPase regulator, which contains a RCCl -type repeat. OMIN also reports that RCCl has associations (as potentially diagnostic, therapeutic, causative, and or related, etc ..) with retinitis pigmentosa (OMIN *312610). Clones in this category include tes3_21d4.

Ras inhibitor proteins: Ras is a signal transducting molecule involved in the receptor tyrosine kinase/RAS/Map kinase signalling cascade. Ras proteins bind GDP/GTP and show intrinsic GTPase activity. Mutations in ras, which change aa 12, 13 or 61 activate the potential of ras to transform cultured cells and are implicated in a variety of human tumours. Ras inhibitor proteins have been associated (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with many disease processes as reported by OMIN including: 1) Tumors of the lung, breast, brain, pituitary, pancrase, bone, skin, bladder, kidney, ovary, prostate and lymphocyte, Melanoma (OMIN *600160); 2) X-linked non-specific mental retardation (OMIN * 300104); 3)adenomatouspolyposis of the colon (OMIN * 175100); 4) Beckwith-Wieddemann Syndrome (#130650); and 5) Major affective disorder 1 (OMIN * 125480). Clones in this category include utel_22g21.

Mammalian proteins cornicon involving the EGF-receptor: Cornicon proteins are part of a signal transduction pathway involving the EGF-receptor. The EGF-receptor has been reported by OMIN to be associated (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with the following diseases: 1) Familial hypercholesterolemia (OMIN 143890); 2) Leprechaunism (OMIN #246200); 3) Hemophilia B (OMIN *306900); 4) Ectodermal dysplasia 1; 5) Kartagenerer syndrome (OMIN *244400) and 6) Glioma of the brain (OMIN * 137800). ). Clones in this category include utel_22el2.

Transmembrane proteins

Membrane region prediction was effected using the ALOM2 software (Klein et al., 1985; version 2 by K. Nakai). Similar to many other methods, the Kyte & Doolitle (1982) amino acid hydrophobicity scale is used in ALOM2 as the primary variable for classifying sequences in terms of their localization. High prediction accuracy is achieved through the system of intelligent decision rules and the utilization of a carefully selected training data set. The method also generates reliability estimates which makes it possible to distinguish between membrane-spanning proteins (I, intrinsic) and globular proteins with regions of high hydrophobicity buried in the core.

For a protein of length L, the block of length / with maximum hydrophobicity is found:

where H, represents the hydrophobicity of an individual residue.

Let P(I/maxΗ) and P(E/maxH) be the conditional probabilities that a protein is integral or peripheral, respectively, given its value of maximal hydrophobicity maxH, and let P(I) and P(E) be the prior probabilities of intrinsic and extrinsic membrane proteins estimated from the training set. Then a sequence is assigned to E if

P(E/maxH) > P(I/maxH)

or, after applying the Bayes rule,

P(E)P(maxH/E) > P(I)P(maxH/I),

where the conditional probabilities P(maxH E) and P(maxH I) can be determined based on the estimates of probability distributions of maxH in both groups.

Discriminant analysis allows to simplify this task by calculating the odds P(E/MaxH):P(I/maxH) as e^b, where b is the left-hand side of a linear or quadratic inequality. For example, for the window of length 17, the protein is allocated to the peripheral category E based on the empirically derived quadratic inequality:

1.05(maxH)²+12.30maxH+17.49 >0,

whereas the optimal inequality for assigning membrane proteins (category I) is linear:

-9.02maxH + 14.27 > 0

The odds parameter can be made more or less stringent. For example, one can require odds at least 1 : 10 for a protein to be classified as integral. This leads to higher selectivity but less sensitivity.

The boundaries of membrane-spanning regions in putative membrane proteins are detected by means of an iterative procedure whereby the most hydrophobic region corresponding to the value maxH is considered to be membrane and removed from the sequence. The classification procedure is then repeated again for the remaining sequence, and, if such a protein is again classified as integral, the next most hydrophobic region is considered. Reference: Klein, P., Kanehisa, M., DeLisi, C. (1985) The detection and classification of membrane-spanning proteins. Biochem Biophys Ada 815: 468-476

Transcription factors

Purified eukaryotic RNA polymerase II is unable to initiate promoter-specific transcription. A family of factors that collectively confer RNAPII promoter specificity is known as the general transcription factors (GTFs). They include the TATA-binding Protein (TBP) TFIIB, TFIIE, TFIIF and TFI IH. These factors are conserved among all eukaryotes.

RNAPII complexes containing the entire set of GTFs or a subset of GTFs together with other proteins have been isolated from mammalian and yeast cells. Although purified RNAPII and GTFs are sufficient for promoter-specific initiation, this system fails to respond to activators. This is mediated by a further complex termed mediator complex which associates with the carboxy-terminal heptapeptide domain (CTD) of the largest subunit of RNAPII.

Purification of human RNAPII complexes resulted in two distinct forms of human RNAPII after analysis of functional properties. One complex contained chromatin remodeling activities but was devoid of GTFs. The other complex did not contain factors that modify chromatin but contained a subset of SRB/mediator subunits and GTFs and other polypeptides that mediate transcriptional activation, a scenario similar to that reported for yeast.

A complex designated NAT (~2O SU) for negative regulator of transcription contains RNAPII, Cdk8, homologs of the yeast mediator complex as well as Rgrl and SrblO/11 known as negative regulators of transcription.

A complex with striking similar structural and functional properties to NAT has been identified designated SMCC (~15 SU) (SRB/mediator coactivator complex), that can also mediate transcriptional activation.

The SMCC complex includes all reported NAT subunits including subunits of the TRAP complex. TRAP is a coactivator complex isolated on the basis of its interaction with the thyroid hormone receptor. Another coactivator complex DRIP, isolated on the basis of its ability to interact with the vitamin D3 receptor, contains novel subunits as well as subunits of NAT/SMCC and TRAP complexes.

The effects of each of these coactivator complexes is dependent on the TFIID complex. It is not known if the T AF subunits of TFIID are required. It is likely that new coactivator complexes will be uncovered containing both novel and previously defined components.

Beside the huge amount of transcription factors which can be part of the RNAIIP holoenzyme or the coactivator complexes there is an even larger quantity of specific transcription factors binding to promoter elements within the DNA sequences of a given gene leading to activation or repression of transcription. A broad range of cellular responses like differentiation, proliferation, cell death and others are elicited through activating or repressing the transcription of target genes.

There are at least five superclasses of transcription factors:

1. Superclass contains members with characteristic basic domains:

Members are:

Leucine zipper factors, where the basic domain is followed by a leucine zipper of repeated leucine residues at every seventh position. The zipper mediates protein dimerization as a prerequisite for DNA-binding.

Helix-loop-helix factors (bHLH) contain a DNA-binding basic region followed by a motif of two potential amphipathic alpha-helices connected by a loop of variable length also mediating dimerization.

Factors with a combination of Helix-loop-helix and leucine zipper.

Further members of this superclass are NF-1, RF-X, and bHSH like proteins.

2. Superclass comprises factors containing zinc-coordinating DNA-binding domains.

Members are: Proteins with Cys4 zinc finger of nuclear receptor type, where two such motifs differing in size, composition and function are present in each receptor molecule. Each finger comprises 4 cysteine residues coordinating one zinc ion. The second half including the second cysteine pair has alpha-helix conformation and the helix of the first finger binds to the DNA through the major groove. The sequence between the first two cysteines of the second finger mediates dimerization upon DNA-binding. This class includes the steroid hormone receptors and the thyroid hormone receptor-like factors. Other diverse cys4 zinc fingers have a motif of GATA-type.

Proteins with Cys2His2 zinc finger domain(s). Each finger comprises 2 cysteine and 2 histidine residues coordinating one zinc ion, and in some cases one histidine is replaced by another cysteine. The zinc ion is essential for DNA-binding.

Proteins with Cys6 cysteine-zinc cluster(s). Six cysteine residues coordinate two zinc ions, i. e. two of the thiol groups are coordinating two zinc ions each. Present in many fungal regulators.

Zinc fingers of alternating composition.

3. Superclass contains factors of helix-turn-helix type.

Members are:

Proteins with homeo domains. Homeo domains are three consecutive alpha-helix structures. Helix 3 contacts mainly the major groove of the DNA, some contacts at the minor groove are observed as well. Helix 2 and 3 resemble the helix-turn-helix structure of prokaryotic regulators.

Proteins with Paired box domain(s). This is a DNA-binding domain of approximately 130 amino acid residues. Its N-terminal half is basic, its C-terminal half is highly charged in general. It probably comprises 3 alpha-helices.

Proteins with Fork head / winged helix domain(s). This domain was identified by homology between HNF-3A and fkh. The domain comprises approx. 110 AA. Analysis of the crystal structure has revealed a compact structure of three alpha-helices, the third alpha-helix being exposed towards the major groove of the DNA. The domain also exerts minor groove contacts. Upon binding to DNA, it induces a bend of 13 degree.

Heat shock factors

Proteins with Tryptophan clusters. The tryptophan clusters comprise several tryptophan residues with a spacing of 12-21 amino acid residues; the subclass of myb-type DNA-binding domains typically exhibit a spacing of 19-21 amino acid residues.

Proteins with TEA domain(s). The TEA domain has been identified as a region which is conserved among the transcription factors TEF-1, TEC1 and abaA. This domain in TEF-1 has been shown to interact with DNA, although two additional regions may also contribute to DNA-binding. It is predicted to fold into three alpha-helices, with a randomly coiled region of 16-18 amino acid residues between helices 1 and 2, and a short stretch between helices 2 and 3 of 3-8 residues.

4. Superclass contains beta-Scaffold Factors with Minor Groove Contacts

Members are:

Proteins with RHR (Rel homology) region.

The structure of the Rel-type DBD exhibits a bipartite subdomain structure, each subdomain comprising a beta-barrel with five loops that form an extensive contact surface to the major groove of the DNA. Particularly, the first loop of the N-terminal subdomain (the highly conserved recognition loop) performs contacts with the recognition element on the DNA, but other loops are involved. The fact that the main DNA-contacts are made through loops has been suggested to provide a high degree of flexibility in binding to a range of different target sequences. Augmenting interactions are achieved by two alpha-helices within the N-terminal Part that form strong minor groove contacts to the A/T-rich center of the B- element. In p65, the sequence between both alpha-helices is much shorter and even helix 2 is truncated. The second, C-terminal domain is necessary mainly for protein dimerization.

p53 proteins MADS (MCMl-agamous-deficiens-SRF) box proteins. Proteins of this class comprise a region of homology. The DNA-binding domain also comprises the dimerization capability. In the DNA-bound dimer (shown for SRF), two antiparallel amphipathic alpha-helices (alpha- I), form a coiled coil and are oriented approximately parallel on the minor groove. These helices make minor and major groove contacts, the N-terminal extensions form minor groove contacts. The bound DNA is bent and wrapped around the protein. It exhibits a compressed minor groove in the center and widened minor groove in the flanks.

Beta-Barrel alpha-helix transcription factors.

TATA-binding proteins

HMG proteins

Proteins of this class comprise a region of homology with the chromosomal non- histone HMG proteins such as HMG1. This region comprises the DNA-binding domain which in some instances such as HMG1 mediates sequence-unspecific, in other cases such LEF-1 sequence-specific binding to DNA. This domain exhibits a typical L-shaped conformation made up of 3 alpha-helices and an extended N-terminal extension of the first helix. The latter together with helix 1, which contains a kink, form the long arm of the L, whereas helices 1 and 2 form the short arm. Binding to the minor groove induces a sharp bending of the DNA by more than 90 degree, away from the bound protein. The overall topology of the DNA-protein complexes resembles somewhat that of the TBP-TATA box complex.

Heteromeric CCAAT factors

Proteins with Grainyhead domain(s)

Cold-shock domain factors. Cold-shock domain proteins are characterized by a highly conserved region first found in prokaryotic cold-shock proteins. This domain is a single- stranded nucleic acid-binding structure interacting with DNA or RNA. It consists of an antiparallel five-stranded beta-barrel, the strands of which are connected by turns and loops. Within this structure, a three-stranded beta-strand contains a conserved RNA-binding motif, RNP1. Not all CSD proteins are transcription factors. Those which specifically bind to a certain sequence are termed Y-box proteins. Proteins of this class were previously called protamine-like domain proteins because of having a highly positively charged domain with interspersed proline residues.

Proteins with Runt homology domain

The members of this transcription factor class have been identified on the basis of their homology to a defined region within the Drosophilia protein Runt. The runt domain is part of the DNA-binding domain of these factors. It consists mainly of beta-strands, does not contain alpha-helical regions and seems to be most similar to the palm domain found in DNA polymerase beta (rat).

5. Superclass contains other transcription factors like Copper fist proteins. HMGKY). STAT. Pocket domain proteins and Ap2/EREBP-related factors.

The classification of transcription factors originates from TRANSFAC database:

http: //transfac.gbf.de/TRANSFAC/

Reference: Heinemeyer

Several categories of proteins are coded for by clones of the invention within the overall group of "Transcription Factors".and include, among others, the following:

Dcoh: Dcoh is a bifunctional protein, complexed with biopterin. It serves as dimerization cofactor of hepatocyte nuclear factor- 1 and catalyzes the dehydration of the biopterin cofactor of phenylalanine hydroxylase. The Dcoh protein has been reported by OMIN to be associated (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with the following diseases: 1) hyperphenylalanemia (OMIN 126090, #264070). Clones in this category include fkd2_46kl2.

Signal transducing proteins: Beta-transducin subunits of G-proteins contain WD-40 repeats. The beta subunits seem to be required for the replacement of GDP by GTP as well as for membrane anchoring and receptor recognition. Due to the zinc finger the novel protein seems to be a new molecule involved in signal transduction and transcription. These proteins have been reported by OMIN to be associated (as potentially diagnostic, therapeutic, causative, and or related, etc...) with the following diseases: 1) essential hypertension (OMIN *139130). Clones in this category include utel_H2. * * *

The invention, therefore, specifically contemplates the following assemblages of materials, which track the above- identified fourteen functional groupings, that are useful in practicing the profiling aspects of the invention. One type of assemblage is nucleic acid- based and can include the following groupings of sequences and their derivatives: all sequences; human fetal brain sequences; brain derived sequences; human fetal kidney library sequences; kidney derived sequences; human mammary carcinoma library sequences; mammary carcinoma derived sequences; human testis library sequences; testes derived sequences; cell cycle genes; cell structure and motility genes; differentiation and development genes; intracellular transport and trafficking genes; metabolism genes; nucleic acid management genes; signal transduction genes; transmembrane protein genes; and transcription factor genes. Other assemblages contain proteins or their corresponding antibodies or antibody fragments, divided along the same groupings.

Database Applications

Because they are human genes and gene products, the inventive molecules are useful as members of a database. Such a database may be used, for example, in drug discovery and rationale drug design or in testing the novelty and non-obviousness of newly sequenced materials. In addition, they are particularly suited in designing variants for the profiling (and other) applications described herein. Hence, the following discussion of electronic embodiments applies equally to such variants, which, naturally, will be generated and stored using a computer using known methodologies.

Accordingly, one aspect of the invention contemplates a database of at least one of the inventive sequences stored on computer readable media. Again, the individual sequences may be grouped with regard to the individual functional and structural groups mentioned above. While the individual sequences of a database may exist in printed form, they are preferably in electronic form, as in an ascii or a text file. They may also exist as word processing files or they may be stored in database applications like DB2, Sybase, Oracle, GCG and GenBank. One skilled in the art will understand the range of applications suitable for using and storing the electronic embodiments of the invention.

"Computer readable media" refers to any medium which can be read and accessed by a computer. These include: magnetic storage media, like floppy discs, hard drives and magnetic tape; optical storage media, like CD-ROM; electrical storage media, like RAM and ROM; and hybrids of these categories, like magnetic/optical storage media. One skilled in the art will readily understand the scope of computer readable media and how to implement them.

Biological Activities and Assays for Implementing Therapeutic and Diagnostic Applications

This section provides assays for biological activity that are useful in characterizing and quantifying the biological activity of the inventive molecules and their derivatives, which is relevant to the pharmacological effects of the inventive molecules. As used in this section, it will be understood that "protein" may also refer to the inventive antibodies (including fragments).

Cytokine and Cell Proliferation/Differentiation Activity

A protein of the present invention may exhibit cytokine, cell proliferation (either inducing or inhibiting) or cell differentiation (either inducing or inhibiting) activity or may induce production of other cytokines in certain cell populations. Many protein factors discovered to date, including all known cytokines, have exhibited activity in one or more factor dependent cell proliferation assays, and hence the assays serve as a convenient confirmation of cytokine activity. The activity of a protein of the present invention is evidenced by any one of a number of routine factor dependent cell proliferation assays for cell lines including, without limitation, 32D, DA2, DA1G, T10, B9, B9/11, BaF3, MC9/G, M + (preB M + ), 2E8, RB5, DAI, 123, T1165, HT2, CTLL2, TF-1, Mo7e and CMK.

The activity of a protein of the invention may, among other means, be measured by the following methods:

Assays for T-cell or thymocyte proliferation include without limitation those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, W. Strober, Pub. Greene Publishing Associates and Wiley- Interscience (Chapter 3, In Vitro assays for Mouse Lymphocyte Function 3.1-3.19; Chapter 7, Immunologic studies in Humans); Takai et al., J. Immunol. 137:3494-3500, 1986; Bertagnolli et al., J. Immunol. 145:1706-1712, 1990; Bertagnolli et al., Cellular Immunology 133:327-341, 1991; Bertagnolli, et al., I. Immunol. 149:3778-3783, 1992; Bowman et al., I. Immunol. 152:1756-1761, 1994. Assays for cytokine production and/or proliferation of spleen cells, lymph node cells or thymocytes include, without limitation, those described in: Polyclonal T cell stimulation, Kruisbeek, A. M. and Shevach, E. M. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 3.12.1-3.12.14, John Wiley and Sons, Toronto. 1994; and Measurement of mouse and human interleukin gamma , Schreiber, R. D. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 6.8.1-6.8.8, John Wiley and Sons, Toronto. 1994.

Assays for proliferation and differentiation of hematopoietic and lymphopoietic cells include, without limitation, those described in: Measurement of Human and Murine Interleukin 2 and Interleukin 4, Bottomly, K., Davis, L. S. and Lipsky, P. E. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 6.3.1-6.3.12, John Wiley and Sons, Toronto. 1991; deVries et al., J. Exp. Med. 173: 1205-1211, 1991; Moreau et al., Nature 336:690-692, 1988; Greenberger et al., Proc. Natl. Acad. Sci. U.S.A. 80:2931- 2938, 1983; Measurement of mouse and human interleukin 6-Nordan, R. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 6.6.1-6.6.5, John Wiley and Sons, Toronto. 1991; Smith et al., Proc. Natl. Aced. Sci. U.S.A. 83: 1857-1861, 1986; Measurement of human Interleukin 11-Bennett, F., Giannotti, J.; Clark, S. C. and Turner, K. J. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 6.15.1 John Wiley and Sons, Toronto. 1991; Measurement of mouse and human Interleukin 9-Ciarletta, A., Giannotti, J., Clark, S. C. and Turner, K. J. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 6.13.1, John Wiley and Sons, Toronto. 1991.

Assays for T-cell clone responses to antigens (which will identify, among others, proteins that affect APC-T cell interactions as well as direct T-cell effects by measuring proliferation and cytokine production) include, without limitation, those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, W Strober, Pub. Greene Publishing Associates and Wiley- Interscience (Chapter 3, In Vitro assays for Mouse Lymphocyte Function; Chapter 6, Cytokines and their cellular receptors; Chapter 7, Immunologic studies in Humans); Weinberger et al., Proc. Natl. Acad. Sci. USA 77:6091-6095, 1980; Weinberger et al., Eur. J. Immun. 11:405-411, 1981; Takai et al., J. Immunol. 137:3494-3500, 1986; Takai et al., J. Immunol. 140:508-512, 1988. Immune Stimulating or Suppressing Activity

A protein of the present invention may also exhibit immune stimulating or immune suppressing activity, including without limitation the activities for which assays are described herein. A protein may be useful in the treatment of various immune deficiencies and disorders (including severe combined immunodeficiency (SOD)), e.g., in regulating (up or down) growth and proliferation of T and/or B lymphocytes, as well as effecting the cytolytic activity of NK cells and other cell populations. These immune deficiencies may be genetic or be caused by vital (e.g., HIV) as well as bacterial or fungal infections, or may result from autoimmune disorders. More specifically, infectious diseases causes by viral, bacterial, fungal or other infection may be treatable using a protein of the present invention, including infections by HIV, hepatitis viruses, herpesviruses, mycobacteria, Leishmania spp., malaria spp. and various fungal infections such as candidiasis. Of course, in this regard, a protein of the present invention may also be useful where a boost to the immune system generally may be desirable, i.e., in the treatment of cancer.

Autoimmune disorders which may be treated using a protein of the present invention include, for example, connective tissue disease, multiple sclerosis, systemic lupus erythematosus, rheumatoid arthritis, autoimmune pulmonary inflammation, Guillain-Barre syndrome, autoimmune thyroiditis, insulin dependent diabetes mellitis, myasthenia gravis, graft-versus-host disease and autoimmune inflammatory eye disease. Such a protein of the present invention may also to be useful in the treatment of allergic reactions and conditions, such as asthma (particularly allergic asthma) or other respiratory problems. Other conditions, in which immune suppression is desired (including, for example, organ transplantation), may also be treatable using a protein of the present invention.

Using the proteins of the invention it may also be possible to modify immune responses, in a number of ways. Down regulation may be in the form of inhibiting or blocking an immune response already in progress or may involve preventing the induction of an immune response. The functions of activated T cells may be inhibited by suppressing T cell responses or by inducing specific tolerance in T cells, or both. Immunosuppression of T cell responses is generally an active, non-antigen-specific, process which requires continuous exposure of the T cells to the suppressive agent. Tolerance, which involves inducing non-responsiveness or anergy in T cells, is distinguishable from immunosuppression in that it is generally antigen-specific and persists after exposure to the tolerizing agent has ceased. Operationally, tolerance can be demonstrated by the lack of a T cell response upon reexposure to specific antigen in the absence of the tolerizing agent.

Down regulating or preventing one or more antigen functions (including without limitation B lymphocyte antigen functions (such as, for example, B7)), e.g., preventing high level lymphokine synthesis by activated T cells, will be useful in situations of tissue, skin and organ transplantation and in graft-versus-host disease (GVHD). For example, blockage of T cell function should result in reduced tissue destruction in tissue transplantation. Typically, in tissue transplants, rejection of the transplant is initiated through its recognition as foreign by T cells, followed by an immune reaction that destroys the transplant. The administration of a molecule which inhibits or blocks interaction of a B7 lymphocyte antigen with its natural ligand(s) on immune cells (such as a soluble, monomeric form of a peptide having B7-2 activity alone or in conjunction with a monomeric form of a peptide having an activity of another B lymphocyte antigen (e.g., B7- 1, B7-3) or blocking antibody), prior to transplantation can lead to the binding of the molecule to the natural ligand(s) on the immune cells without transmitting the corresponding costimulatory signal. Blocking B lymphocyte antigen function in this matter prevents cytokine synthesis by immune cells, such as T cells, and thus acts as an immunosuppressant. Moreover, the lack of costimulation may also be sufficient to anergize the T cells, thereby inducing tolerance in a subject. Induction of long-term tolerance by B lymphocyte antigen-blocking reagents may avoid the necessity of repeated administration of these blocking reagents. To achieve sufficient immunosuppression or tolerance in a subject, it may also be necessary to block the function of a combination of B lymphocyte antigens.

The efficacy of particular blocking reagents in preventing organ transplant rejection or GVHD can be assessed using animal models that are predictive of efficacy in humans. Examples of appropriate systems which can be used include allogeneic cardiac grafts in rats and xenogeneic pancreatic islet cell grafts in mice, both of which have been used to examine the immunosuppressive effects of CTLA4Ig fusion proteins in vivo as described in Lenschow et al., Science 257:789-792 (1992) and Turka et al., Proc. Natl. Acad. Sci USA, 89:11102-11105 (1992). In addition, murine models of GVHD (see Paul ed., Fundamental Immunology, Raven Press, New York, 1989, pp. 846-847) can be used to determine the effect of blocking B lymphocyte antigen function in vivo on the development of that disease. Blocking antigen function may also be therapeutically useful for treating autoimmune diseases. Many autoimmune disorders are the result of inappropriate activation of T cells that are reactive against self tissue and which promote the production of cytokines and autoantibodies involved in the pathology of the diseases. Preventing the activation of autoreactive T cells may reduce or eliminate disease symptoms. Administration of reagents which block costimulation of T cells by disrupting receptor: ligand interactions of B lymphocyte antigens can be used to inhibit T cell activation and prevent production of autoantibodies or T cell-derived cytokines which may be involved in the disease process. Additionally, blocking reagents may induce antigen-specific tolerance of autoreactive T cells which could lead to long-term relief from the disease. The efficacy of blocking reagents in preventing or alleviating autoimmune disorders can be determined using a number of well-characterized animal models of human autoimmune diseases. Examples include murine experimental autoimmune encephalitis, systemic lupus erythmatosis in MRL/lpr/lpr mice or NZB hybrid mice, murine autoimmune collagen arthritis, diabetes mellitus in NOD mice and BB rats, and murine experimental myasthenia gravis (see Paul ed., Fundamental Immunology, Raven Press, New York, 1989, pp. 840-856).

Upregulation of an antigen function (preferably a B lymphocyte antigen function), as a means of up regulating immune responses, may also be useful in therapy. Upregulation of immune responses may be in the form of enhancing an existing immune response or eliciting an initial immune response. For example, enhancing an immune response through stimulating B lymphocyte antigen function may be useful in cases of viral infection. In addition, systemic viral diseases such as influenza, the common cold, and encephalitis might be alleviated by the administration of stimulatory forms of B lymphocyte antigens systemically.

Alternatively, anti-vital immune responses may be enhanced in an infected patient by removing T cells from the patient, costimulating the T cells in vitro with viral antigen- pulsed APCs either expressing a peptide of the present invention or together with a stimulatory form of a soluble peptide of the present invention and reintroducing the in vitro activated T cells into the patient. Another method of enhancing anti-viral immune responses would be to isolate infected cells from a patient, transfect them with a nucleic acid encoding a protein of the present invention as described herein such that the cells express all or a portion of the protein on their surface, and reintroduce the transfected cells into the patient. The infected cells would now be capable of delivering a costimulatory signal to, and thereby activate, T cells in vivo.

In another application, up regulation or enhancement of antigen function (preferably B lymphocyte antigen function) may be useful in the induction of tumor immunity. Tumor cells (e.g., sarcoma, melanoma, lymphoma, leukemia, neuroblastoma, carcinoma) transfected with a nucleic acid encoding at least one peptide of the present invention can be administered to a subject to overcome tumor-specific tolerance in the subject. If desired, the tumor cell can be transfected to express a combination of peptides. For example, tumor cells obtained from a patient can be transfected ex vivo with an expression vector directing the expression of a peptide having B7-2-like activity alone, or in conjunction with a peptide having B7-l-like activity and/or B7-3-like activity. The transfected tumor cells are returned to the patient to result in expression of the peptides on the surface of the transfected cell. Alternatively, gene therapy techniques can be used to target a tumor cell for transfection in vivo.

The presence of the peptide of the present invention having the activity of a B lymphocyte antigen(s) on the surface of the tumor cell provides the necessary costimulation signal to T cells to induce a T cell mediated immune response against the transfected tumor cells. In addition, tumor cells which lack MHC class I or MHC class II molecules, or which fail to reexpress sufficient mounts of MHC class I or MHC class II molecules, can be transfected with nucleic acid encoding all or a portion of (e.g. , a cytoplasmic-domain truncated portion) of an MHC class I alpha chain protein and beta 2 microglobulin protein or an MHC class II alpha chain protein and an MHC class II beta chain protein to thereby express MHC class I or MHC class II proteins on the cell surface. Expression of the appropriate class I or class II MHC in conjunction with a peptide having the activity of a B lymphocyte antigen (e.g., B7-1, B7-2, B7-3) induces a T cell mediated immune response against the transfected tumor cell. Optionally, a gene encoding an antisense construct which blocks expression of an MHC class II associated protein, such as the invariant chain, can also be cotransfected with a DNA encoding a peptide having the activity of a B lymphocyte antigen to promote presentation of tumor associated antigens and induce tumor specific immunity. Thus, the induction of a T cell mediated immune response in a human subject may be sufficient to overcome tumor-specific tolerance in the subject. The activity of a protein of the invention may, among other means, be measured by the following methods:

Suitable assays for thymocyte or splenocyte cytotoxicity include, without limitation, those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, W. Strober, Pub. Greene Publishing Associates and Wiley-Interscience (Chapter 3, In Vitro assays for Mouse Lymphocyte Function 3.1-3.19; Chapter 7, Immunologic studies in Humans); Herrmann et al., Proc. Natl. Acad. Sci. USA 78:2488-2492, 1981; Herrmann et al., J. Immunol. 128: 1968-1974, 1982; Handa et al., J. Immunol. 135:1564-1572, 1985; Takai et al., I. Immunol. 137:3494- 3500, 1986; Takai et al., J. Immunol. 140:508-512, 1988; Herrmann et al., Proc. Natl. Acad. Sci. USA 78:2488-2492, 1981; Herrmann et al., J. Immunol. 128:1968-1974, 1982; Handa et al., J. Immunol. 135: 1564-1572, 1985; Takai et al., J. Immunol. 137:3494-3500, 1986; Bowmanet al., J. Virology 61:1992-1998; Takai et al., J. Immunol. 140:508-512, 1988; Bertagnolli et al. , Cellular Immunology 133:327-341, 1991; Brown et al., J. Immunol. 153:3079-3092, 1994.

Assays for T-cell-dependent immunoglobulin responses and isotype switching (which will identify, among others, proteins that modulate T-cell dependent antibody responses and that affect Thl/Th2 profiles) include, without limitation, those described in: Maliszewski, J. Immunol. 144:3028-3033, 1990; and Assays for B cell function: In vitro antibody production, Mond, J. J. and Brunswick, M. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 3.8.1-3.8.16, John Wiley and Sons, Toronto. 1994.

Mixed lymphocyte reaction (MLR) assays (which will identify, among others, proteins that generate predominantly Thl and CTL responses) include, without limitation, those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, W. Strober, Pub. Greene Publishing Associates and Wiley-Interscience (Chapter 3, In Vitro assays for Mouse Lymphocyte Function 3.1-3.19; Chapter 7, Immunologic studies in Humans); Takai et al., J. Immunol. 137:3494-3500, 1986; Takai et al., J. Immunol. 140:508-512, 1988; Bertagnolli et al., J. Immunol. 149:3778-3783, 1992.

Dendritic cell-dependent assays (which will identify, among others, proteins expressed by dendritic cells that activate naive T-cells) include, without limitation, those described in: Guery et al., J. Immunol. 134:536-544, 1995; Inaba et al., Journal of Experimental Medicine 173:549-559, 1991; Macatonia et al. , Journal of Immunology 154:5071-5079, 1995; Porgador et al., Journal of Experimental Medicine 182:255-260, 1995; Nair et al., Journal of Virology 67:4062-4069, 1993; Huang et al., Science 264:961- 965, 1994; Macatonia et al., Journal of Experimental Medicine 169:1255-1264, 1989; Bhardwaj et al., Journal of Clinical Investigation 94:797-807, 1994; and Inaba et al., Journal of Experimental Medicine 172:631-640, 1990.

Assays for lymphocyte survival/apoptosis (which will identify, among others, proteins that prevent apoptosis after superantigen induction and proteins that regulate lymphocyte homeostasis) include, without limitation, those described in: Darzynkiewicz et al., Cytometry 13:795-808, 1992; Gorczyca et al., Leukemia 7:659-670, 1993; Gorczyca et al., Cancer Research 53: 1945-1951, 1993; Itoh et al., Cell 66:233-243, 1991; Zacharchuk, Journal of Immunology 145:4037-4045, 1990; Zamai et al., Cytometry 14:891-897, 1993; Gorczyca et al., International Journal of Oncology 1:639-648, 1992.

Assays for proteins that influence early steps of T-cell commitment and development include, without limitation, those described in: Antica et al., Blood 84:111-117, 1994; Fine et al., Cellular Immunology 155:111-122, 1994; Galy et al., Blood 85:2770-2778, 1995; Toki et al., Proc. Nat. Acad Sci. USA 88:7548-7551, 1991.

Hematopoiesis Regulating Activity

A protein of the present invention may be useful in regulation of hematopoiesis and, consequently, in the treatment of myeloid or lymphoid cell deficiencies. Even marginal biological activity in support of colony forming cells or of factor-dependent cell lines indicates involvement in regulating hematopoiesis, e.g. in supporting the growth and proliferation of erythroid progenitor cells alone or in combination with other cytokines, thereby indicating utility, for example, in treating various anemias or for use in conjunction with irradiation/chemotherapy to stimulate the production of erythroid precursors and/or erythroid cells; in supporting the growth and proliferation of myeloid cells such as granulocytes and monocytes/macrophages (i.e., traditional CSF activity) useful, for example, in conjunction with chemotherapy to prevent or treat consequent myelo- suppression; in supporting the growth and proliferation of megakaryocytes and consequently of platelets thereby allowing prevention or treatment of various platelet disorders such as thrombocytopenia, and generally for use in place of or complimentary to platelet transfusions; and/or in supporting the growth and proliferation of hematopoietic stem cells which are capable of maturing to any and all of the above-mentioned hematopoietic cells and therefore find therapeutic utility in various stem cell disorders (such as those usually treated with transplantation, including, without limitation, aplastic anemia and paroxysmal nocturnal hemoglobinuria), as well as in repopulating the stem cell compartment post irradiation/chemotherapy, either in-vivo or ex-vivo (i.e., in conjunction with bone marrow transplantation or with peripheral progenitor cell transplantation (homologous or heterologous)) as normal cells or genetically manipulated for gene therapy.

The activity of a protein of the invention may, among other means, be measured by the following methods:

Suitable assays for proliferation and differentiation of various hematopoietic lines are cited above.

Assays for embryonic stem cell differentiation (which will identify, among others, proteins that influence embryonic differentiation hematopoiesis) include, without limitation, those described in: Johansson et al. Cellular Biology 15:141-151, 1995; Keller et al., Molecular and Cellular Biology 13:473-486, 1993; McClanahan et al., Blood 81:2903- 2915, 1993. Assays for stem cell survival and differentiation (which will identify, among others, proteins that regulate lympho-hematopoiesis) include, without limitation, those described in: Methylcellulose colony forming assays, Freshney, M. G. In Culture of Hematopoietic Cells. R. I. Freshney, et al. eds. Vol pp. 265-268, Wiley-Liss, Inc., New York, N.Y. 1994; Hirayama et al., Proc. Natl. Acad. Sci. USA 89:5907-5911, 1992; Primitive hematopoietic colony forming cells with high proliferative potential, McNiece, I. K. and Briddell, R. A. In Culture of Hematopoietic Cells. R. I. Freshney, et al. eds. Vol pp. 23- 39, Wiley-Liss, Inc., New York, N.Y. 1994; Neben et al., Experimental Hematology 22:353-359, 1994; Cobblestone area forming cell assay, Ploemacher, R. E. In Culture of Hematopoietic Cells. R. I. Freshney, et al. eds. Vol pp. 1-21, Wiley-Liss, Inc., New York, N.Y. 1994; Long term bone marrow cultures in the presence of stromal cells, Spooncer, E., Dexter, M. and Allen, T. In Culture of Hematopoietic Cells. R. I. Freshney, et al. eds. Vol pp. 163-179, Wiley-Liss, Inc., New York, N.Y. 1994; Long term culture initiating cell assay, Sutherland, H. J. In Culture of Hematopoietic Cells. R. I. Freshney, et al. eds. Vol pp. 139-162, Wiley-Liss, Inc., New York, N.Y. 1994.

Tissue Growth Activity

A protein of the present invention also may have utility in compositions used for bone, cartilage, tendon, ligament and/or nerve tissue growth or regeneration, as well as for wound healing and tissue repair and replacement, and in the treatment of burns, incisions and ulcers.

A protein of the present invention, which induces cartilage and/or bone growth in circumstances where bone is not normally formed, has application in the healing of bone fractures and cartilage damage or defects in humans and other animals. Such a preparation employing a protein of the invention may have prophylactic use in closed as well as open fracture reduction and also in the improved fixation of artificial joints. De novo bone formation induced by an osteogenic agent contributes to the repair of congenital, trauma induced, or oncologic resection induced craniofacial defects, and also is useful in cosmetic plastic surgery.

A protein of this invention may also be used in the treatment of periodontal disease, and in other tooth repair processes. Such agents may provide an environment to attract bone-forming cells, stimulate growth of bone-forming cells or induce differentiation of progenitors of bone-forming cells. A protein of the invention may also be useful in the treatment of osteoporosis or osteoarthritis, such as through stimulation of bone and/or cartilage repair or by blocking inflammation or processes of tissue destruction (collagenase activity, osteoclast activity, etc.) mediated by inflammatory processes.

Another category of tissue regeneration activity that may be attributable to the protein of the present invention is tendon/ligament formation. A protein of the present invention, which induces tendon/ligament-like tissue or other tissue formation in circumstances where such tissue is not normally formed, has application in the healing of tendon or ligament tears, deformities and other tendon or ligament defects in humans and other animals. Such a preparation employing a tendon ligament-like tissue inducing protein may have prophylactic use in preventing damage to tendon or ligament tissue, as well as use in the improved fixation of tendon or ligament to bone or other tissues, and in repairing defects to tendon or ligament tissue. De novo tendon ligament-like tissue formation induced by a composition of the present invention contributes to the repair of congenital, trauma induced, or other tendon or ligament defects of other origin, and is also useful in cosmetic plastic surgery for attachment or repair of tendons or ligaments. The compositions of the present invention may provide environment to attract tendon- or ligament-forming cells, stimulate growth of tendon- or ligament-forming cells, induce differentiation of progenitors of tendon- or ligament-forming cells, or induce growth of tendon/ligament cells or progenitors ex vivo for return in vivo to effect tissue repair. The compositions of the invention may also be useful in the treatment of tendonitis, carpal tunnel syndrome and other tendon or ligament defects. The compositions may also include an appropriate matrix and/or sequestering agent as a carrier as is well known in the art.

The protein of the present invention may also be useful for proliferation of neural cells and for regeneration of nerve and brain tissue, i.e. for the treatment of central and peripheral nervous system diseases and neuropathies, as well as mechanical and traumatic disorders, which involve degeneration, death or trauma to neural cells or nerve tissue. More specifically, a protein may be used in the treatment of diseases of the peripheral nervous system, such as peripheral nerve injuries, peripheral neuropathy and localized neuropathies, and central nervous system diseases, such as Alzheimer's, Parkinson's disease, Huntington's disease, amyotrophic lateral sclerosis, and Shy-Drager syndrome. Further conditions which may be treated in accordance with the present invention include mechanical and traumatic disorders, such as spinal cord disorders, head trauma and cerebro vascular diseases such as stroke. Peripheral neuropathies resulting from chemotherapy or other medical therapies may also be treatable using a protein of the invention.

Proteins of the invention may also be useful to promote better or faster closure of non-healing wounds, including without limitation pressure ulcers, ulcers associated with vascular insufficiency, surgical and traumatic wounds, and the like.

It is expected that a protein of the present invention may also exhibit activity for generation or regeneration of other tissues, such as organs (including, for example, pancreas, liver, intestine, kidney, skin, endothelium), muscle (smooth, skeletal or cardiac) and vascular (including vascular endothelium) tissue, or for promoting the growth of cells comprising such tissues. Part of the desired effects may be by inhibition or modulation of fibrotic scarring to allow normal tissue to regenerate. A protein of the invention may also exhibit angiogenic activity.

A protein of the present invention may also be useful for gut protection or regeneration and treatment of lung or liver fibrosis, reperfusion injury in various tissues, and conditions resulting from systemic cytokine damage.

A protein of the present invention may also be useful for promoting or inhibiting differentiation of tissues described above from precursor tissues or cells; or for inhibiting the growth of tissues described above.

The activity of a protein of the invention may, among other means, be measured by the following methods:

Assays for tissue generation activity include, without limitation, those described in: International Patent Publication No. WO95/ 16035 (bone, cartilage, tendon); International Patent Publication No. WO95/05846 (nerve, neuronal); International Patent Publication No. WO91/07491 (skin, endothelium).

Assays for wound healing activity include, without limitation, those described in: Winter, Epidermal Wound Healing, pps. 71-112 (Maibach, H. I. and Rovee, D. T., eds.), Year Book Medical Publishers, Inc., Chicago, as modified by Eaglstein and Mertz, J. Invest. Dermatol 71:382-84 (1978).

Activin/Inhibin Activity

A protein of the present invention may also exhibit activin- or inhibin-related activities. Inhibins are characterized by their ability to inhibit the release of follicle stimulating hormone (FSH), while activins and are characterized by their ability to stimulate the release of follicle stimulating hormone (FSH). Thus, a protein of the present invention, alone or in heterodimers with a member of the inhibin alpha family, may be useful as a contraceptive based on the ability of inhibins to decrease fertility in female mammals and decrease spermatogenesis in male mammals. Administration of sufficient amounts of other inhibins can induce infertility in these mammals. Alternatively, the protein of the invention, as a homodimer or as a heterodimer with other protein subunits of the inhibin- beta group, may be useful as a fertility inducing therapeutic, based upon the ability of activin molecules in stimulating FSH release from cells of the anterior pituitary. See, for example, U.S. Pat. No. 4,798,885. A protein of the invention may also be useful for advancement of the onset of fertility in sexually immature mammals, so as to increase the lifetime reproductive performance of domestic animals such as cows, sheep and pigs.

^" The activity of a protein of the invention may, among other means, be measured by the following methods:

Assays for activin/ inhibin activity include, without limitation, those described in: Vale et al., Endocrinology 91:562-572, 1972; Ling et al., Nature 321:779-782, 1986; Vale et al., Nature 321:776-779, 1986; Mason et al., Nature 318:659-663, 1985; Forage et al., Proc. Natl. Acad. Sci. USA 83:3091-3095, 1986.

Chemotactic/Chemokinetic Activity

A protein of the present invention may have chemotactic or chemokinetic activity (e.g., act as a chemokine) for mammalian cells, including, for example, monocytes, fibroblasts, neutrophils, T-cells, mast cells, eosinophils, epithelial and/or endothelial cells. Chemotactic and chemokinetic proteins can be used to mobilize or attract a desired cell population to a desired site of action. Chemotactic or chemokinetic proteins provide particular advantages in treatment of wounds and other trauma to tissues, as well as in treatment of localized infections. For example, attraction of lymphocytes, monocytes or neutrophils to tumors or sites of infection may result in improved immune responses against the tumor or infecting agent.

A protein or peptide has chemotactic activity for a particular cell population if it can stimulate, directly or indirectly, the directed orientation or movement of such cell population. Preferably, the protein or peptide has the ability to directly stimulate directed movement of cells. Whether a particular protein has chemotactic activity for a population of cells can be readily determined by employing such protein or peptide in any known assay for cell chemotaxis.

The activity of a protein of the invention may, among other means, be measured by the following methods:

Assays for chemotactic activity (which will identify proteins that induce or prevent chemotaxis) consist of assays that measure the ability of a protein to induce the migration of cells across a membrane as well as the ability of a protein to induce the adhesion of one cell population to another cell population. Suitable assays for movement and adhesion include, without limitation, those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Marguiles, E. M. Shevach, W. Strober, Pub. Greene Publishing Associates and Wiley-Interscience (Chapter 6.12, Measurement of alpha and beta Chemokines 6.12.1-6.12.28; Taub et al. J. Clin. Invest. 95:1370-1376, 1995; Lind et al. APMIS 103:140-146, 1995; Muller et al Eur. J. Immunol. 25: 1744-1748; Gruber et al. J. of Immunol. 152:5860-5867, 1994; Johnston et al. J. of Immunol. 153: 1762-1768, 1994.

Hemostatic and Thrombolytic Activity

A protein of the invention may also exhibit hemostatic or thrombolytic activity. As a result, such a protein is expected to be useful in treatment of various coagulation disorders (including hereditary disorders, such as hemophilias) or to enhance coagulation and other hemostatic events in treating wounds resulting from trauma, surgery or other causes. A protein of the invention may also be useful for dissolving or inhibiting formation of thromboses and for treatment and prevention of conditions resulting therefrom (such as, for example, infarction of cardiac and central nervous system vessels (e.g., stroke).

The activity of a protein of the invention may, among other means, be measured by the following methods:

Assay for hemostatic and thrombolytic activity include, without limitation, those described in: Linet et al., J. Clin. Pharmacol. 26: 131-140, 1986; Burdick et al., Thrombosis Res. 45:413-419, 1987; Humphrey et al., Fibrinolysis 5:71-79 (1991); Schaub, Prostaglandins 35:467-474, 1988.

Receptor/Ligand Activity

A protein of the present invention may also demonstrate activity as receptors, receptor ligands or inhibitors or agonists of receptor/ligand interactions. Examples of such receptors and ligands include, without limitation, cytokine receptors and their ligands, receptor kinases and their ligands, receptor phosphatases and their ligands, receptors involved in cell-cell interactions and their ligands (including without limitation, cellular adhesion molecules (such as selectins, integrins and their ligands) and receptor/ligand pairs involved in antigen presentation, antigen recognition and development of cellular and humoral immune responses). Receptors and ligands are also useful for screening of potential peptide or small molecule inhibitors of the relevant receptor/ligand interaction. A protein of the present invention (including, without limitation, fragments of receptors and ligands) may themselves be useful as inhibitors of receptor/ligand interactions.

The activity of a protein of the invention may, among other means, be measured by the following methods:

Suitable assays for receptor-ligand activity include without limitation those described imCurrent Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, W. Strober, Pub. Greene Publishing Associates and Wiley- Interscience (Chapter 7.28, Measurement of Cellular Adhesion under static conditions 7.28.1-7.28.22), Takai et al., Proc. Natl. Acad. Sci. USA 84:6864-6868, 1987; Bierer et al., J. Exp. Med. 168:1145-1156, 1988; Rosenstein et al., J. Exp. Med. 169:149-160 1989; Stoltenborg et al., J. Immunol. Methods 175:59-68, 1994; Stitt et al., Cell 80:661- 670, 1995.

Anti-Inflammatory Activity

Proteins of the present invention may also exhibit anti-inflammatory activity. The anti-inflammatory activity may be achieved by providing a stimulus to cells involved in the inflammatory response, by inhibiting or promoting cell-cell interactions (such as, for example, cell adhesion), by inhibiting or promoting chemotaxis of cells involved in the inflammatory process, inhibiting or promoting cell extravasation, or by stimulating or suppressing production of other factors which more directly inhibit or promote an inflammatory response. Proteins exhibiting such activities can be used to treat inflammatory conditions including chronic or acute conditions), including without limitation intimation associated with infection (such as septic shock, sepsis or systemic inflammatory response syndrome (SIRS)), ischemia-reperfusion injury, endotoxin lethality, arthritis, complement- mediated hyperacute rejection, nephritis, cytokine or chemokine-induced lung injury, inflammatory bowel disease, Crohn's disease or resulting from over production of cytokines such as TNF or IL-1. Proteins of the invention may also be useful to treat anaphylaxis and hypersensitivity to an antigenic substance or material.

Tumor Inhibition Activity

In addition to the activities described above for immunological treatment or prevention of tumors, a protein of the invention may exhibit other anti-tumor activities. A protein may inhibit tumor growth directly or indirectly (such as, for example, via ADCC). A protein may exhibit its tumor inhibitory activity by acting on tumor tissue or tumor precursor tissue, by inhibiting formation of tissues necessary to support tumor growth (such as, for example, by inhibiting angiogenesis), by causing production of other factors, agents or cell types which inhibit tumor growth, or by suppressing, eliminating or inhibiting factors, agents or cell types which promote tumor growth.

Other Activities

A protein of the invention may also exhibit one or more of the following additional activities or effects: inhibiting the growth, infection or function of, or killing, infectious agents, including, without limitation, bacteria, viruses, fungi and other parasites; effecting (suppressing or enhancing) bodily characteristics, including, without limitation, height, weight, hair color, eye color, skin, fat to lean ratio or other tissue pigmentation, or organ or body part size or shape (such as, for example, breast augmentation or diminution, change in bone form or shape); effecting biorhythms or caricadic cycles or rhythms; effecting the fertility of male or female subjects; effecting the metabolism, catabolism, anabolism, processing, utilization, storage or elimination of dietary fat, lipid, protein, carbohydrate, vitamins, minerals, cofactors or other nutritional factors or component(s); effecting behavioral characteristics, including, without limitation, appetite, libido, stress, cognition (including cognitive disorders), depression (including depressive disorders) and violent behaviors; providing analgesic effects or other pain reducing effects; promoting differentiation and growth of embryonic stem cells in lineages other than hematopoietic lineages; hormonal or endocrine activity; in the case of enzymes, correcting deficiencies of the enzyme and treating deficiency-related diseases; treatment of hyperproliferative disorders (such as, for example, psoriasis); immunoglobulin-like activity (such as, for example, the ability to bind antigens or complement); and the ability to act as an antigen in a vaccine composition to raise an immune response against such protein or another material or entity which is cross-reactive with such protein.

Particular Applications for Certain Clones

The following sets out a non-exclusive list of applications for certain embodiments of the invention. In the interest of economy, applications relevant to multiple embodiments are not duplicated in this list. Other embodiments described in below have similar characteristics, as described therein. The artisan is directed, therefore, to this section for similar descriptions of the functions of other embodiment. Testes htes3_l 5c24: The new protein can find application in modulation of 2-hydroxyacid dehydrogenases-dependent pathways and as a new enzyme for biotechnologic production processes. htes3_15i5: The new protein can find application in modulating the structure of the human spermatozoa radia spoke head and modulation of sperm motility in men. htes3_15kl 1 : The novel protein contains a protein kinase ATP-binding region signature and a serine/threonine protein kinase active-site signature. The new protein can find application in modulation of intracellular signal pathways dependent on this kinase. htes3_17nl2: The new protein can find application in modulating/blocking the expression of SOX-controlled genes. htes3_20k2: The new protein can find application as a target for the development of new nociception-modulating drugs. htes3_20ml8: The new protein can find application in modulation of mitochondrial DNA replication and maintenance. htes3_20d4: The new protein can find application in the regulation of gene expression by activition of nuclear GTP-binding proteins. The X-linked retinitis pigmentosa is a result of a defect GTPase regulator, which contains a RCCl -type repeat. htes3_21jl5: NY-CO-33 is a protein recognised by autologous antibodies of human colon cancer patients. The novel protein contains 4 C2H2 Zinc fingers and is a new putativ transcription factor. The new protein can find application in modulating/blocking the expression of genes controlled by this transcription factor.

The new protein can find application in modulating chromosome transport in mitosis and meiosis and modulation of cell division. htes3_26g22: The new protein can find application in modulating chromosome transport in mitosis and meiosis and modulation of cell division. The novel TBP- binding protein is considered to participate in transcription regulation through the interaction with TBP. The new protein can find application in modulation of gene transcription. htes3_21116: The new protein can find application in modulation of protein translocation into the endoplasmic reticulum. htes3_27dl : The novel protein can find application in modulation of ubiquitin- and protein metabolism in cells. htes3_2ml8: The novel protein can find application as multifunctional nuclease / exoribonuclease. htes3_35b4: The new protein can find application in modulation of the mitotic spindle. htes3_35b5: The novel protein can find application in modulating the v-ATPase activity in endocytic and secretory organelles. htes3_35e21 : Due to the close relationship to human interleukin-7, the novel interleukin is expected to act as a new growth factor for human B lineage cells. Additionally, the protein should induce the gene rearrangement of the T-cell receptor repertoire, leading to thymocyte commitment, and subsequently induce both cytotoxic T-cell- and lymphocyte-activated killer cells. This new interleukin could find clinical application in a variety of conditions of hematolymphopoietic failure and different tumours, because of its recruitment of B cell lineage cells, cytotoxic T-cell- and lymphocyte-activated killer cells. htes3_35kl6: Therefore it is a new fatty acid-Co A synthetasese/ligase with unknown substrate. The new protein can find application in modulation of fatty acid metabolism and as a new enzyme for biotechnologic production processes. htes3_35nl2: The new protein can find application in modulation of ADP-transport and energy metabolism in cells/mitochondria. htes3_35n9: The new protein can find application in modulation of carboxylester metabolism and as a new enzyme for biotechnologic production processes. htes3_35p22: The novel protein is closely raleted to human tre-2 and other enzymes involved in the degradation of ubiquitinated proteins. The human tre-2 oncogene encodes a deubiquitinating enzyme, indicating a role for the ubiquitin system in mammalian growth control. The novel protein can find application in cancer diagnostics and treatment, and in regulating protein stability and growth control via regulation of ubiquitination. htes3_4h6: The novel kinesin protein can find application in modulating the function of kinesin and modulating intracellular transport via/on microtubules. htes3_72kl5: FGDl -related F-actin-binding protein (Farbin/FGDl) is a novel F-actin- binding protein. The gene locus fgdl seems to be responsible for faciogenital dysplasia or Aarskog-Scott syndrome. Frabin binds F-actin and shows F-actin-cross- linking activity. Overexpression of frabin in Swiss 3T3 cells and COS7 cells induces cell shape change and c-Jun N-terminal kinase activation, as described for FGDl. Because FGDl has been shown to serve as a GDP/GTP exchange protein for Cdc42 small G protein, it is likely that frabin is a direct linker between Cdc42 and the actin cytoskeleton. Cdc42p is an esin yeast, Cdc42p transduces signals to the actin cytoskeleton to initiate and maintain polarized growth and to mitogen-activated protein morphogenesis. In mammalian cells, Cdc42p regulates a variety of actin- dependent events and induces the JNK/SAPK protein kinase cascade, which leads to the activation of transcription factors within the nucleus. The novel protein seems to be the human orthologue of rat frabin.

The new protein can find application in modulating of cell structure and motility as well as modulation of the JNK/SAPK pathway. htes3_72pl6: As Mem3, the novel protein is similar to yeast VPS (vacuolar protein sorting) 35. The null allele of VPS35 results in yeast in a differential defect in the sorting of vacuolar carboxypeptidase Y (CPY), proteinase A (PrA), proteinase B (PrB), and alkaline phosphatase (ALP). The new protein can find application in modulation the sorting of proteins into different compartments. htes3_7b22: The novel protein is related to paramyosin, a major structural component of thick filaments and invertebrate muscle. Paramyosins are promising antigens for immunization against several parasites, such as Schistosoma mansoni. The new protein can find application in modulating cell adhesion/motility and membrane/cyto skeleton structure and dynamic. htes3_7j3: The new protein is closely related to C-Takl and therefore should be involved in cell-cycle regulation, too. The new protein can find application in modulating/blocking the cell cycle. htes3_7p9: The nuclear domain (ND)10 also described as POD or Kr bodies is involved in the development of acute promyelocytic leukemia and virus-host interactions. The NDP52 protein is part of this complex structure. In vivo, NDP52 is transcribed in all human tissues, but is redistributed upon viral infection and interferon treatment. ND10 plays an important role in the viral life cycle. The novel protein is similar to NDP52. It contains three leucine zippers and a RGD cell attachment site. This protein seems to be a novel part of the ND819) complex. The new protein can find application in modulation of viral infections and tumour events. htes3_8ml0: The poly(A)-binding protein (PABP) binds to the messenger (mRNA) 3'-poly(A) tail found on most eukaryotic mRNAs and together with the poly(A) tail has been implicated in governing the stability and the translation of mRNA. The new protein can find application in modulation of mRNA translation and processing/stability.

Kidney hfkd2_24bl5: The new protein can find application in modulation of hexose metabolism pathways and as a new enzyme for biotechnologic production processes. hfkd2_24n20: The new protein seems to be part of the signalling pathway between tyrosine kinases and the membrane/cyto skeleton. The new protein can find application in modulating cell adhesion/motility and membrane/cyto skeleton structure and dynamics. hfkd2_3ol7: The new protein can find application in modulation of the respiratory electron transport chain pathways of mitochondria. hfkd2_46j20: The new protein can find application in modulating the homoprotocatechuate degradative pathway and as a enzyme for biotechnologic production processes. hfkd2_46kl9: The new protein can find application in modulating/blocking the expression of genes controlled by the hepatocyte nuclear factor- 1. hfkd2_46m4: SARI proteins are involved in vesicular transport between the endoplasmic reticulum and the Golgi apparatus. hfkd2_46kl4: rab6 is a ubiquitous ras-like GTPase involved in intra-Golgi transport. The new protein can find application in modulating the transport of vesicles inside the Golgi apparatus.

Uterus Associated: hutel_18il9: The SREBP-2 protein is embedded in the membranes of the nucleus and endoplasmic reticulum. In cholesterol-depleted cells the proteins are cleaved to release soluble NH2 -terminal fragments that enter the nucleus and activate genes encoding the low density lipoprotein receptor and enzymes of cholesterol synthesis. The new protein is a putative transcription factor capable of protein-protein interaction via a lim domain and additionally shows similarity to the common sunflower transcription factor SF3. hute 1_1811 : The novel protein is similar to several 40S ribosomal proteins and therefore seems to part of the corresponding ribosome sub-unit. hute l_19g22: The new protein can find application in modulation of tissue- calcification, especially the uterus. hutel_19hl7: The new protein can find application in modulating the response of cells to oxysterols. hute l_20bl9: The novel protein seems to be a novel enzyme with sarcosine oxidase activity. The new protein can find application in modulation of sarcosine metabolism and as a new enzyme for biotechnologic production processes. hute l_20g21 : The novel protein seems to be a new ras inhibitor protein. The new protein can find application in modulating/blocking ras dependent signal transduction pathways. hute l_20hl3: The novel protein is a new human alpha-adaptin. The new protein can find application in modulating endocytosis and vesicle trafficking in cells. hute l_20ml 1 : The new protein can find application in modulating/blocking the activity of protein phosphatase- 1 and in modulating the cell cycle. hute l_20m24: This protein is a putative mannosyl transferase that is involved in the assembly of the core oligosaccharide Glc3Man9GlcNAc2. The new protein can find application in modulation of glycosylation of proteins and as a new enzyme for biotechnologic production processes. hute l_22el2: The new protein can find application in modulating the cornichon modulated signal transduction way and also the EGF receptor signaling processes. hute l_23el3: The novel protein contains a serine protease of the subtilase family with an aspartic acid-containing active site. The new protein can find application in modulation of proteinase activity in cells and as a new enzyme for proteomics and biotechnologic production processes. hutel_24j6: The new protein can find application in modulation of cell-cell-adhesion. hutel_24h3: The new protein can find application as a useful marker for chondro- osteogenic cell differentiation and for the modulation of chondro-osteogenic cell differentiation.

Fetal Brain: hfbr2_16cl6: The new protein can find application in modulating/blocking of cyto skeleton-membrane protein interaction. hfbr2_23b21 : The new protein can find application in modulating/blocking the guanylate cyclase-pathway. hfbr2_23bl0: The new protein can find application in modulation of splicing. hfbr2_2b5: The novel protein contains the typical (xxG)n repeat of collagen proteins and a Pfam von Willebrand factor type A domain. Therefore, the protein seems to be a new collagen alpha chain. The new protein can find application in modulation of connective tissue, bone and cartilage development and maintainance. hfbr2_2cl7: The new protein can find application in modulating/blocking G-protein- dependent pathways. hfbr2_2dl5: The new protein can find application in modulating early spermatogenesis. hfbr2_2il7: The new protein can find clinical application in modulating the transport of glycoproteins inside cells, especially of the LDL receptor. hfbr2_2kl4: Tumour-suppressor genes are known to be involved in the control of cell growth and division, interacting with proteins which control the cell cycle. The N33 gene is significantly methylated in tumour cells, a mechanism by which tumor- suppressor genes are inactivated in cancer. In addition, the novel protein contains a RGD cell attachment site. Therefore the novel protein is a new putative tumour- suppressor gene. hfbr_3cl8: RNA helicases comprise a large family of proteins that are involved in basic biological systems such as nuclear and mitochondrial splicing processes, RNA editing, rRNA processing, translation initiation, nuclear mRNA export, and mRNA degradation. RNA helicases are essential factors in cell development and differentiation, and some of them play a role in transcription and replication of viral single-stranded RNA genomes. The members of the largest subgroup, the DEAD and DEAH box proteins, exhibit a strong dependence of the unwinding activity on ATP hydrolysis. The novel protein contains a DEAD-box and is a new member of this subgroup. hfbr_3g8: The new protein can find application modulating NAT assembly and action and therefore be important in metabolism of drugs and environmental mutagens. hfbr2_62bl 1 : The rac small GTPase is associated with type-I phosphatidylinositol 4- phosphate 5-kinase and regulating the production of phosphatidylinositol 4,5- bisphosphate. The new protein is expected to activate p21 rac -related small GTPases. hfbr2_62ol7: The new protein can find application in modulation of cholesterol binding and transport by LDL-receptors and LDL-binding proteins. hfbr_6b24: The new protein can find application in modulation of rhamnose metabolism and as a new enzyme for biotechnologic production processes. hfbr_72bl8: The new protein can find application in modulating DNA repair and mutagenesis. hfbr_78c4: The new protein can find application in modulating/blocking the response of cells to interferons. hfbr_78k24: These enzymes are involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. The new protein can find application in modulation of protein stability /degradation in cells. hfbr_82e4: The new protein can find clinical application in modulating/blocking calmodulin-mediated pathways in human neuronal cells.

VARIANTS OF THE INVENTIVE DNA MOLECULES

Variants in General

"Variants," according to the invention, include DNA and/or protein molecules that resemble, structurally and/or functionally, those set forth in herein. Variants may be isolated from natural sources ("homologs"), may be entirely synthetic or may be based in part on both natural and synthetic approaches.

The section set forth below presents various structural and functional characteristics of molecules within the invention. Preferred molecules are characterized by a combination of one or more of these characteristics. For instance, some preferred molecules are described with reference to at least two structural characteristics, while others may be described with reference to at least one structural and at least one functional characteristic.

It will be recognized by the skilled artisan that structure ultimately defines function, i.e. the functions of the molecules described herein derives from the structures of those molecules. Accordingly, the structural variants described below that bear the closest structural relationship (as variously defined below) to the inventive molecules are the variants that most likely will preserve biological function. This relationship between structure and function will guide the skilled artisan in identifying the preferred embodiments of the invention.

Splicing Variants

It is well-known that eukaryotic structural genes are comprised of both protein coding and non-coding portions. When the messenger RNA is transcribed from the DNA template, it contains introns, which are non-coding, and exons, which are coding. In order to form a translation competent mRNA, the introns must be "spliced" out of this initial pre mRNA.

Specific sequences within the pre mRNA represent "splice junctions" that direct the cellular splicing machinery to the appropriate position. The splice junctions are loosely conserved sequence regions of the pre mRNA, which almost invariably begin with GT and end with AG (DNA perspective). The 5' end of the splice junction typically contains about nine somewhat conserved residues, for example, C/AAGTA/G AGT . The 3' end usually contains a pyrimidine rich stretch of at least about 11 nucleotides, followed by NC/TAGG. Splicing occurs before the GT and after the AG. Mount, Nucleic Acids Res. 10:459-72 (1982).

Interestingly, exons often correspond to discrete functional domains of the protein product. The intron exon arrangement thus creates a linear array of nucleotides which can be correlated to discrete, and often interchangeable, functional protein fragments. Go, Nature 291:90-92 (1981); Branden et al , EMBO J. 3:1307-10 (1984). This linear arrangement creates the possibility of generating multiple different full length proteins by rearranging the order of the different functional portions in the array. For example, if a set of exons are arranged 1-2-3-4, where (-) represents the introns separating the exons, a splicing event need not simply produce 1234, but may produce 123, 134, 124 and so on. Production of different mRNA products in this way is commonly called "alternative splicing. " Andreadiset al. , Ann. Rev. Cell Biol. 3:207-42 (1987).

Some of the present DNA molecules can be represented in modular fashion in terms of their coding regions. Essentially, these modules are exons (though each "exon" may in fact be made up of several exons), which may be combined in different ways to form a variety of different DNA molecules, each encoding a different functional protein. Splicing variants are indicated below.

Degenerate Variants

One aspect of the present invention provides "degenerate variants" of the nucleic acid fragments of the present invention. A "degenerate variant" is a nucleotide fragment which differs from those of inventive molecules by nucleotide sequence, but due to the degeneracy of the genetic code, encodes an identical polypeptide sequence.

Given the known relationship between DNA sequences and the proteins they encode, degenerate variants typically are described by reference to this relationship. It is well known that the degeneracy of the genetic code results in many possible DNA sequences which encode a particular protein. Indeed, of the three bases which comprise an amino acid- encoding triplet, the third position, and often the second, almost always may vary. This fact alone allows for a class of variant DNA molecules which encode protein sequences identical to those disclosed herein, yet have about 30% sequence variation. In other words, the variant DNA molecules are about 70% identical to the inventive DNAs, having no additional or deleted sequences. Thus, one aspect of the invention provides degenerate variant DNA molecules encoding the inventive protein sequences.

In one embodiment, these variants have at least about 70% sequence identity with the DNA molecules described herein. In a preferred embodiment, these variants have at least about 80% sequence identity to the inventive molecules. In a more preferred embodiment these variants have at least about 90% sequence identity with the inventive molecules.

Conservative Amino Acid Variants

Variants according to the invention also may be made that conserve the overall molecular structure of the encoded proteins. Given the properties of the individual amino acids comprising the disclosed protein products, some rational substitutions will be recognized by the skilled worker. Amino acid substitutions, i.e. "conservative substitutions," may be made, for instance, on the basis of similarity in polarity, charge, solubility, hydrophobicity, hydrophilicity, and/or the amphipathic nature of the residues involved.

For example: (a) nonpolar (hydrophobic) amino acids include alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, and methionine; (b) polar neutral amino acids include glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine; (c) positively charged (basic) amino acids include arginine, lysine, and histidine; and (d) negatively charged (acidic) amino acids include aspartic acid and glutamic acid. Substitutions typically may be made within groups (a)-(d). In addition, glycine and proline may be substituted for one another based on their ability to disrupt α-helices. Similarly, certain amino acids, such as alanine, cysteine, leucine, methionine, glutamic acid, glutamine, histidine and lysine are more commonly found in α-helices, while valine, isoleucine, phenylalanine, tyrosine, tryptophan and threonine are more commonly found in β-pleated sheets. Glycine, serine, aspartic acid, asparagine, and proline are commonly found in turns. Some preferred substitutions may be made among the following groups: (i) S and T; (ii) P and G; and (iii) A, V, L and I. Given the known genetic code, and recombinant and synthetic DNA techniques, the skilled scientist readily can construct DNAs encoding the conservative amino acid variants.

As used herein, "sequence identity" between two polypeptide sequences indicates the percentage of amino acids that are identical between the sequences. "Sequence similarity" indicates the percentage of amino acids that either are identical or that represent conservative amino acid substitutions.

Functionally Equivalent Variants

Yet another class of DNA variants within the scope of the invention may be described with reference to the product they encode. As shown below, some of the inventive DNA molecules encode a protein having a degree of homology with known proteins, or protein domains. It is expected, therefore, that they will have some or all of the requisite functional features of such molecules. These "functionally equivalent variants" products are characterized by the fact that they are functionally equivalent, with respect to biological activity, to certain known molecules.

The instant invention provides information on common structural motifs, including consensus sequences that will guide the artisan in constructing functionally equivalent variants. It will be understood that the motifs, identified for each inventive protein, may be modified within the identified consensus sequences. Thus, the invention contemplates the proteins disclosed herein that contain variability in the consensus sequences identified, and the invention further contemplates the full range of nucleic acids encoding them, and the complements of those nucleic acids. Hybridizing Variants

DNA variants within the invention also may be described by reference to their physical properties in hybridization. One skilled in the field will recognize that DNA can be used to identify its complement and, since DNA is double stranded, its equivalent or homolog, using nucleic acid hybridization techniques. It will also be recognized that hybridization can occur with less than 100% complementarity. However, given appropriate choice of conditions, hybridization techniques can be used to differentiate among DNA sequences based on their structural relatedness to a particular probe. For guidance regarding such conditions see, for example, Sambrook et al , 1989, MOLECULAR CLONING, A LABORATORY MANUAL, Cold Spring Harbor Press, N.Y. ; and Ausubel et al, 1989, CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, Green Publishing Associates and Wiley Interscience, N.Y.

Structural relatedness between two polynucleotide sequences can be expressed as a function of "stringency" of the conditions under which the two sequences will hybridize with one another. As used herein, the term "stringency" refers to the extent that the conditions disfavor hybridization. Stringent conditions strongly disfavor hybridization, and only the most structurally related molecules will hybridize to one another under such conditions. Conversely, non-stringent conditions favor hybridization of molecules displaying a lesser degree of structural relatedness. Hybridization stringency, therefore, directly correlates with the structural relationships of two nucleic acid sequences. The following relationships are useful in correlating hybridization and relatedness (where T_m is the melting temperature of a nucleic acid duplex):

a. T_m = 69.3 + 0.41(G+C)%

b. The T_m of a duplex DNA decreases by 1°C with every increase of 1 % in the number of mismatched base pairs.

c μ₂ - (T_m)_μ, = 18.5 lθg₁₀μ2/μl where μl and μ2 are the ionic strengths of two solutions.

Hybridization stringency is a function of many factors, including overall DNA concentration, ionic strength, temperature, probe size and the presence of agents which disrupt hydrogen bonding. Factors promoting hybridization include high DNA concentrations, high ionic strengths, low temperamres, longer probe size and the absence of agents that disrupt hydrogen bonding.

Hybridization usually is done in two stages. First, in the "binding" stage, the probe is bound to the target under conditions favoring hybridization. Stringency is usually controlled at this stage by altering the temperature. For high stringency, the temperature is usually between 65°C and 70°C, unless short (<20 nt) oligonucleotide probes are used. A representative hybridization solution comprises 6X SSC, 0.5% SDS, 5X Denhardt's solution and lOOμg of non-specific carrier DNA. See Ausubel et al , supra, section 2.9, supplement 27 (1994). Of course many different, yet functionally equivalent, buffer conditions are known. Where the degree of relatedness is lower, a lower temperature may be chosen. Low stringency binding temperatures are between about 25°C and 40°C. Medium stringency is between at least about 40°C to less than about 65°C. High stringency is at least about 65°C.

Second, the excess probe is removed by washing. It is at this stage that more stringent conditions usually are applied. Hence, it is this "washing" stage that is most important in determining relatedness via hybridization. Washing solutions typically contain lower salt concentrations. One exemplary medium stringency solution contains 2X SSC and 0.1 % SDS. A high stringency wash solution contains the equivalent (in ionic strength) of less than about 0.2X SSC, with a preferred stringent solution containing about 0. IX SSC. The temperatures associated with various stringencies are the same as discussed above for "binding. " The washing solution also typically is replaced a number of times during washing. For example, typical high stringency washing conditions comprise washing twice for 30 minutes at 55° C. and three times for 15 minutes at 60° C.

The present invention includes nucleic acid molecules that hybridize to the inventive molecules under high stringency binding and washing conditions. More preferred molecules (from an mRNA perspective) are those that are at least 50 % of the length of any one of those depicted in below. Particularly preferred molecules are at least 75 % of the length of those molecules.

Substitutions, Insertions, Additions and Deletions

In a general sense, the preferred DNA variants of the invention are those that retain the closest relationship, as described by "sequence identity" to the inventive DNA molecules. According to another aspect of the invention, therefore, substitutions, insertions, additions and deletions of defined properties are contemplated. It will be recognized that sequence identity between two polynucleotide sequences, as defined herein, generally is determined with reference to the protein coding region of the sequences. Thus, this definition does not at all limit the amount of DNA, such as vector DNA, that may be attached to the molecules described herein. Preferred DNA sequence variants include molecules encoding proteins sharing some or all of any relevant biological activity of the native molecule.

In creating these variants, the skilled worker will be guided by reference to the protein structure. First, insertions and deletions in any recognized functional domain, above, generally should be avoided, except as noted below in the section entitled "Proteins," where this domain is discussed in detail. Alterations in such domains usually will be limited to conservative amino acid substitutions. In addition, where insertions and deletions are desired, this may be accomplished at the N- and/or C-terminus of the protein molecule (or the corresponding coding regions of the DNA). If insertions or deletions are made within the protein, deletions of major structural features usually should be avoided. Thus, a preferred place to make insertion or deletion variants is in non-structural regions, such as linker regions between two alpha helices.

"Substitutions" generally refer to alterations in the DNA sequence which do not change its overall length, but only alter one or more nucleotide positions, substituting one for another in the common sense of the word. One class of preferred substitutions, "degenerate substitutions, " are those that do not alter the encoded amino acid sequence. Some subsitutions retains 50%, 55%, 60% or 65% identity. Preferred substitutions retain at least about 70% identity, more preferably at least 70% or 75% identity, with the inventive DNAs. Some more preferred molecules have at least about 80% identity, more preferably at least 80% or 85% identity. Particularly preferred DNAs share at least about 90% identity, more preferably at least 90% or 95% identity.

"Insertions," unlike substitutions, alter the overall length of the DNA molecule, and thus sometimes the encoded protein. Insertions add extra nucleotides to the interior (not the 5' or 3' ends) of the subject DNAs. Preferred insertions are made with reference to the protein sequence encoded by the DNA. Thus, it is most preferred to provide an insertion in the DNA at a location that corresponds to an area of the encoded protein which lacks structure. For instance, it typically would not be beneficial, if the preservation of biological activity is desired, to provide an insertion within an alpha-helical region or a beta-pleated sheet. Accordingly, non-structural areas, such as those containing helix-breaking glycines and proline residues, are most preferred sites of insertion. Other preferred sites of insertion are the splice sites, which are indicated above in the description of the inventive DNA molecules.

While the optimal size of insertions will vary depending upon the site of insertion and its effect on the overall conformation of the encoded protein, some general guides are useful. Generally, the total insertions (irrespective of their number) should not add more than about 30% (or preferably not more than 30%) to the overall size of the encoded protein. More preferably, the insertion adds less than about 10-20% (yet more preferably 10-20%) in size, with less than about 10% being most preferred. The number of insertions is limited only by the number of suitable insertions sites, and secondarily by the foregoing size preferences.

"Additions," like insertions, also add to the overall size of the DNA molecule, and usually the encoded protein. However, instead of being made within the molecule, they are made on the 5' or 3' end, usually corresponding to the N- or C- terminus of the encoded protein. Unlike deletions, additions are not very size-dependent. Indeed, additions may be of virtually any size. Preferred additions, however, do not exceed about 100% of the size of the native molecule. More preferably, they add less than about 60 to 30% to the overall size, with less than about 30% being most preferred.

"Deletions" diminish the overall size of the DNA and, therefore, also reduce the size of the protein encoded by that DNA. Deletions may be made from either end of the molecule or internal to it. Typical preferred deletions remove discrete structural features of the encoded protein. For example, some deletions will comprise the deletion of one or more exons which may define a structural feature. Preferred deletions remove less than about 30% of the size of the subject molecule. More preferred deletions remove less than about 20% and most preferred deletions remove less than about 10% .

Computer-Defined Variants and Definition of "Sequence Identity "

In general, both the DNA and protein molecules of the invention can be defined with reference to "sequence identity. " As used herein, "sequence identity" refers to a comparison made between two molecules using, for example, the standard Smith- Waterman algorithm that is well known in the art.

Some molecules have at lease about 50%, 55% or 60% identity. Preferred molecules are those having at least about 65% sequence identity, more preferably at least 65% or 70% sequence identity. Other preferred molecules have at least about 80%, more preferably at least 80% or 85%, sequence identity. Particularly preferred molecules have at least about 90% sequence identity, more preferably at least 90% sequence identity. Most preferred molecules have at least about 95 % , more preferably at least 95 % , sequence identity. As used herein, two nucleic acid molecules or proteins are said to "share significant sequence identity" if the two contain regions which possess greater than 85 % sequence (amino acid or nucleic acid) identity.

"Sequence identity" is defined herein with reference the Blast 2 algorithm, which is available at the NCBI (http://www.ncbi.nlm.nih.gov/BLAST), using default parameters. References pertaining to this algorithm include: those found at http://www.ncbi.nlm.nih.gov/BLAST/blast_references.html; Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990) "Basic local alignment search tool." J. Mol. Biol. 215:403-410; Gish, W. & States, D.J. (1993) "Identification of protein coding regions by database similarity search." Nature Genet. 3:266-272; Madden, T.L., Tatusov, RL. & Zhang, J. (1996) "Applications of network BLAST server" Meth. Enzymol. 266:131-141; Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D.J. (1997) "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs." Nucleic Acids Res. 25:3389-3402; and Zhang, J. & Madden, T.L. (1997) "PowerBLAST: A new network BLAST application for interactive or automated sequence analysis and annotation." Genome Res. 7:649-656.

METHODS OF MAKING VARIANTS

It will be recognized that variants of the inventive molecules can be constructed in several different ways. For example, they may be constructed as completely synthetic DNAs. Methods of efficiently synthesizing oligonucleotides in the range of 20 to about 150 nucleotides are widely available. See Ausubel et al , supra, section 2.11, Supplement 21 (1993). Overlapping oligonucleotides may be synthesized and assembled in a fashion first reported by Khorana et al, J. Mol. Biol. 72:209-217 (1971); see also Ausubel et al, Section 8.2. The synthetic DNAs are designed with convenient restriction sites engineered at the 5' and 3 ' ends of the gene to facilitate cloning into an appropriate vector.

An alternative method of generating variants is to start with one of the inventive DNAs and then to conduct site-directed mutagenesis. See Ausubel et al , supra, chapter 8, Supplement 37 (1997). In a typical method, a target DNA is cloned into a single-stranded DNA bacteriophage vehicle. Single-stranded DNA is isolated and hybridized with a oligonucleotide containing the desired nucleotide alteration(s). The complementary strand is synthesized and the double stranded phage is introduced into a host. Some of the resulting progeny will contain the desired mutant, which can be confirmed using DNA sequencing. In addition, various methods are available that increase the probability that the progeny phage will be the desired mutant. These methods are well known to those in the field and kits are commercially available for generating such mutants.

ISOLATING HOMOLOGS

Methods

By using the sequences disclosed herein as probes or as primers, and techniques such as PCR cloning and colony /plaque hybridization, one skilled in the art can obtain homologs. "Homologs" are essentially naturally-occurring variants and include allelic, species-specific and tissue-specific variants.

Region-specific primers or probes derived from the nucleotide sequence(s) provided can be used to prime DNA synthesis and PCR amplification, as well as to identify colonies containing cloned DNA encoding a homolog using known methods (Innis et al, PCR Protocols, Academic Press, San Diego, CA (1990)). Such an application is useful in diagnostic methods, as described in more detail below, as well as in preparing full-length DNAs from various sources. The PCR primers are preferably at least 15 bases, and more preferably at least 18 bases in length. When selecting a primer sequence, it is preferred that the primer pairs have approximately the same G/C ratio, so that melting temperatures are approximately the same. As a general guide, the formula 3(G+C) + 2(A+T) = °C, is useful.

When using primers derived from the inventive sequences, one skilled in the art will recognize that by employing high stringency conditions (e.g. , annealing at 50-60°C), only sequences with greater than 75% sequence identity to the primer will be amplified. By employing lower stringency conditions (e.g., annealing at 35-37°C), sequences which have greater than 40-50% sequence identity to the primer also will be amplified.

The PCR product may be subcloned and sequenced to confirm that it indeed displays the expected sequence identity. The PCR fragment may then be used to isolate a full length cDNA clone by a variety of methods. For example, the amplified fragment may be labeled and used to screen a bacteriophage cDNA library. Alternatively, the labeled fragment may be used to screen a genomic library.

PCR technology may also be utilized to isolate full length cDNA sequences. For example, RNA may be isolated, following standard procedures, from an appropriate cellular or tissue source. A reverse transcription reaction may be performed on the RNA using an oligonucleotide primer specific for the most 5 ' end of the amplified fragment for the priming of first strand synthesis. The resulting RNA/DNA hybrid may then be "tailed" with guanines using a standard terminal transferase reaction, the hybrid may be digested with RNAase H, and second strand synthesis may then be primed with a poly-C primer. Thus, cDNA sequences upstream of the amplified fragment may easily be isolated. For a review of cloning strategies which may be used, see e.g. , Sambrook et al. , 1989, supra.

When using DNA probes derived from the inventive sequences for colony/plaque hybridization, one skilled in the art will recognize that by employing medium to high stringency conditions (e.g., hybridizing at 50-65°C in 5X SSPC and 50% formamide, and washing at 50-65°C in 0.5X SSPC), sequences having regions with greater than 90% sequence identity to the probe can be obtained, and that by employing lower stringency conditions (e.g., hybridizing at 35-37°C in 5X SSPC and 40-45% formamide, and washing at 42°C in SSPC), sequences having regions with greater than 35-45% sequence identity to the probe will be obtained.

Suitably, genomic or cDNA libraries can be constructed and screened in accord with the previous paragraph. The libraries should be derived from a tissue or organism that is known to express the gene of interest, or that is suspected of expressing the gene. The clone containing the homolog may then be purified through methods routinely practiced in the art, and subjected to sequence analysis.

Additionally, an expression library can be constructed utilizing DNA isolated from or cDNA synthesized from a tissue or organism that is known to express the gene of interest, or that is suspected of expressing the gene. In this manner, clones may be induced and screened using standard antibody screening techniques in conjunction with antibodies raised against the normal gene product, as described herein. (For screening techniques, see, for example, Harlow, E. and Lane, eds., 1988, ANTIBODIES: A LABORATORY MANUAL, Cold Spring Harbor Press, Cold Spring Harbor Press.)

Human Homologs Any organism or tissue can be used as the source for homologs of the present invention so long as the organism or tissue naturally expresses such a protein or contains genes encoding the same. The most preferred organism for isolating homologs is human.

PROTEINS OF THE INVENTION

One class of proteins included within the invention is encoded by the inventive DNA molecules presented. Other proteins according to the invention are those encoded by the DNA variants described above. As noted, these variants are designed with the encoded proteins in mind.

A preferred class of protein fragments includes those fragments which retain any biological activity. These molecules share functional features common the family of proteins, although these characteristics may vary in degree.

According to one aspect of the invention fragments of the inventive proteins are contemplated. Some preferred fragments are those which are capable of eliciting an immune response. Generally these "antigenic" fragments will be from about five amino acids in length to about fifty amino acids in length. Some preferred antigenic fragments are from five to about twenty amino acids long. "Antigenic" response may refer to a T cell response, a B cell response or a response by cells of the macrophage/monocyte lineages. In most cases, however, it will refer to the immune response involved in the generation of antibodies. In other words, the relevant immune response is that of helper T cells and/or B cells. These preferred molecules comprise one or more T cell and /or B cell epitopes.

ANTIBODIES OF THE INVENTION

Antibodies raised against the proteins and protein fragments of the invention also are contemplated by the invention. Described below are antibody products and methods for producing antibodies capable of specifically recognizing one or more epitopes of the presently described proteins and their derivatives.

Antibodies include, but are not limited to polyclonal antibodies, monoclonal antibodies (mAbs), humanized or chimeric antibodies, single chain antibodies including single chain Fv (scFv) fragments, Fab fragments, F(ab')₂ fragments, fragments produced by a Fab expression library, anti-idiotypic (anti-Id) antibodies, epitope-binding fragments, and humanized forms of any of the above. As known to one in the art, these antibodies may be used, for example, in the detection of a target protein in a biological sample. They also may be utilized as part of treatment methods, and/or may be used as part of diagnostic techniques whereby patients may be tested for abnormal levels or for the presence of abnormal forms of the such proteins.

In general, techniques for preparing polyclonal and monoclonal antibodies as well as hybridomas capable of producing the desired antibody are well known in the art (Campbell, A.M., Monoclonal Antibody Technology: Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier Science Publishers, Amsterdam, The Netherlands (1984); St. Groth et al., /. Immunol. Methods 35:1-21 (1980); Kohler and Milstein, Nature 256:495-497 (1975)), the trioma technique, the human B-cell hybridoma technique (Kozbor et al, Immunology Today 4:72 (1983); Cole et al, in Monoclonal Antibodies and Cancer Therapy, Alan R. Liss, Inc. (1985), pp. 77-96). Antibodies may also be generated by the known techniques of phage display and in vitro immunization.

Polyclonal Antibodies

Polyclonal antibodies are heterogeneous populations of antibody molecules derived from the sera of animals immunized with an antigen, such as an inventive protein or an antigenic derivative thereof.

Polyclonal antiserum, containing antibodies to heterogeneous epitopes of a single protein, can be prepared by immunizing suitable animals with the expressed protein described above, which can be unmodified or modified, as known in the art, to enhance immunogenicity. Immunization methods include subcutaneous or intraperitoneal injection of the polypeptide.

Effective polyclonal antibody production is affected by many factors related both to the antigen and to the host species. For example, small molecules tend to be less immunogenic than other and may require the use of carriers and/or adjuvant. In addition, host animal response may vary with site of inoculation. Both inadequate or excessive doses of antigen may result in low titer antisera. In general, however, small doses (high ng to low μg levels) of antigen administered at multiple intradermal sites appears to be most reliable. Host animals may include but are not limited to rabbits, mice, chickens and rats, to name but a few. An effective immunization protocol for rabbits can be found in Vaitukaitis, J. et αl, J. Clin. Endocrinol. Metαb. 33:988-991 (1971). The protein immunogen may be modified or administered in an adjuvant in order to increase the protein's antigenicity. Methods of increasing the antigenicity of a protein are well known in the art and include, but are not limited to coupling the antigen with a heterologous protein (such as globulin β-galactosidase) or through the inclusion of an adjuvant during immunization. Adjuvants include Freund's (complete and incomplete), mineral gels such as aluminum hydroxide, surface active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, keyhole limpet hemocyanin, dimtrophenol, and potentially useful human adjuvants such as BCG (bacille Calmette-Guerin) and Corynebacteriumparvum.

Booster injections can be given at regular intervals, with at least one usually being required for optimal antibody production. The antiserum may be harvested when the antibody titer begins to fall. Titer may be determined semi-quantitatively, for example, by double immunodiffusion in agar against known concentrations of the antigen. See, for example, Ouchterlony et al, Chap. 19 in: Handbook of Experimental Immunology, Wier, ed, Blackwell (1973). Plateau concentration of antibody is usually in the range of 0.1 to 0.2 mg/ml of serum (about 12 μM). The antiserum may be purified by affinity chromatography using the immobilized immunogen carried on a solid support. Such methods of affinity chromatography are well known in the art.

Affinity of the antisera for the antigen may be determined by preparing competitive binding curves, as described, for example, by Fisher, Chap. 42 in: Manual of Clinical Immunology, second edition, Rose and Friedman, eds., Amer. Soc. For Microbiology, Washington, D.C. (1980).

In addition to using protein an the immunogen, DNA molecules may be used directly. In this manner, a DNA encoding the protein immunogen is administered. Boosting and harvesting is done in a manner analogous to that detailed above. Yet another method of producing antibodies entails immunizing chickens and harvesting the antibodies from their eggs.

Monoclonal Antibodies

Monoclonal antibodies (MAbs), are homogeneous populations of antibodies to a particular antigen. They may be obtained by any technique which provides for the production of antibody molecules by continuous cell lines in culture or in vivo. MAbs may be produced by making hybridomas which are immortalized cells capable of secreting a specific monoclonal antibody.

Monoclonal antibodies to any of the proteins, peptides and epitopes thereof described herein can be prepared from murine hybridomas according to the classical method of Kohler, G. and Milstein, C, Nature 256:495-497 (1975) (and U.S. Patent No. 4,376,110) or modifications of the methods thereof, such as the human B-cell hybridoma technique (Kosbor et al , 1983, Immunology Today 4:72; Cole et al , 1983, Proc. Natl. Acad. Sci. USA 80: 2026-2030), and the EBV-hybridoma technique (Cole et al , 1985, MONOCLONAL ANTIBODIES AND CANCER THERAPY, Alan R. Liss, Inc., pp. 77-96).

In one method a mouse is repetitively inoculated with a few micrograms of the selected protein over a period of a few weeks. The mouse is then sacrificed, and the antibody producing cells of the spleen are isolated.

The spleen cells are fused, typically using polyethylene glycol, with mouse myeloma cells, such as SP2/0-Agl4 myeloma cells. The excess, unfused cells are destroyed by growth of the system on selective media comprising aminopterin (HAT media). The successfully fused cells are diluted, and aliquots are plated to microliter plates where growth is continued.

Antibody-producing clones (hybridomas) are identified by detection of antibody in the supernatant fluid of the wells by immunoassay procedures. These include ELISA, as originally described by Engvall, Meth. Enzymol. 70:419 (1980), western blot analysis, radioimmunoassay (Lutz et al , Exp. Cell Res. 175:109-124 (1988)) and modified methods thereof.

Selected positive clones can be expanded and their monoclonal antibody product harvested for use. Detailed procedures for monoclonal antibody production are described in Davis, L. et al BASIC METHODS IN MOLECULAR BIOLOGY, Elsevier, New York. Section 21-2 (1989). The hybridoma clones may be cultivated w vitro or in vivo, for instance as ascites. Production of high titers of mAbs in vivo makes this the presently preferred method of production. Alternatively, hybridoma culture in hollow fiber bioreactors provides a continuous high yield source of monoclonal antibodies.

The antibody class and subclass may be determined using procedures known in the art (Campbell, A.M. , Monoclonal Antibody Technology: Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier Science Publishers, Amsterdam, The Netherlands (1984)). MAbs may be of any immunoglobulin class including IgG, IgM, IgE, IgA, IgD and any subclass thereof. Methods of purifying monoclonal antibodies are well known in the art.

Antibody Derivatives and Fragments

Fragments or derivatives of antibodies include any portion of the antibody which is capable of binding the target antigen, or a specific portion thereof. Antibody derivatives include poly-specific (e.g., bi-specific) antibodies, which contain binding sites specific for two or more different epitopes. These epitopes may be from the same or different inventive molecules or one or more epitope may be from a molecule not specifically disclosed here.

Antibody fragments specifically include F(ab Fab, Fab' and Fv fragments. These can be generated from any class of antibody, but typically are made from IgG or IgM. They may be made by conventional recombinant DNA techniques or, using the classical method, by proteolytic digestion with papain or pepsin. See CURRENT PROTOCOLS IN IMMUNOLOGY, chapter 2, Coligan et α/. , eds., (John Wiley & Sons 1991-92).

F(ab')₂ fragments are typically about 110 kDa (IgG) or about 150 kDa (IgM) and contain two antigen-binding regions, joined at the hinge by disulfide bond(s). Virtually all, if not all, of the Fc is absent in these fragments. Fab' fragments are typically about 55 kDa (IgG) or about 75 kDa (IgM) and can be formed, for example, by reducing the disulfide bond(s) of an F(ab')₂ fragment. The resulting free sulfhydryl group(s) may be used to conveniently conjugate Fab' fragments to other molecules, such as detection reagents (e.g. , enzymes).

Fab fragments are monovalent and usually are about 50 kDa (from any source). Fab fragments include the light (L) and heavy (H) chain, variable (V_L and V_H, respectively) and constant (C_L C_H, respectively) regions of the antigen-binding portion of the antibody. The H and L portions are linked by an intramolecular disulfide bridge.

Fv fragments are typically about 25 kDa (regardless of source) and contain the variable regions of both the light and heavy chains (V_L and V_H, respectively). Usually, the V_L and V_H chains are held together only by non-covalent interacts and, thus, they readily dissociate. They do, however, have the advantage of small size and they retain the same binding properties of the larger Fab fragments. Accordingly, methods have been developed to crosslink the V_L and V_H chains, using, for example, glutaraldehyde (or other chemical crosslinkers), intermolecular disulfide bonds (by incorporation of cysteines) and peptide linkers. The resulting Fv is now a single chain (i.e. , SCFv). Other antibody derivatives include single chain antibodies (U.S. Patent 4,946,778; Bird, Science 242:423-426 (1988); Huston et al , Proc. Natl. Acad. Sci. USA 85:5879-5883 (1988); and Ward et al. , Nature 334:544-546 (1989)). Single chain antibodies are formed by linking the heavy and light chain fragments of the Fv region via an amino acid bridge, resulting in a single chain FV (SCFv).

One preferred method involves the generation of scFvs by recombinant methods, which allows the generation of Fvs with new specificities by mixing and matching variable chains from different antibody sources. In a typical method, a recombinant vector would be provided which comprises the appropriate regulatory elements driving expression of a cassette region. The cassette region would contain a DNA encoding a peptide linker, with convenient sites at both the 5' and 3' ends of the linker for generating fusion proteins. The DNA encoding a variable region(s) of interest may be cloned in the vector to form fusion proteins with the linker, thus generating an scFv.

In an exemplary alternative approach, DNAs encoding two Fvs may be ligated to the DNA encoding the linker, and the resulting tripartite fusion may be ligated directly into a conventional expression vector. The scFv DNAs generated any of these methods may be expressed in prokaryotic or eukaryotic cells, depending on the vector chosen.

Antibody fragments which recognize specific epitopes may be generated by known techniques. For example, such fragments include but are not limited to: the F(ab'^ fragments which can be produced by pepsin digestion of the antibody molecule and the Fab fragments which can be generated by reducing the disulfide bridges of the F(ab^ fragments. Alternatively, Fab expression libraries may be constructed (Huse et al., 1989, Science, 246: 1275-1281) to allow rapid and easy identification of monoclonal Fab fragments with the desired specificity.

Derivatives also include "chimeric antibodies" (Morrison et al , Proc. Natl. Acad. Sci. , 81:6851-6855 (1984); Neuberger et al , Nature, 312:604-608 (1984); Takeda et al , Nature, 314:452-454 (1985)). These chimeras are made by splicing the DNA encoding a mouse antibody molecule of appropriate specificity with, for instance, DNA encoding a human antibody molecule of appropriate specificity. Thus, a chimeric antibody is a molecule in which different portions are derived from different animal species, such as those having a variable region derived from a murine mAb and a human immunoglobulin constant region. These are also known sometimes as "humanized" antibodies and they offer the added advantage of at least partial shielding from the human immune system. They are, therefore, particularly useful in therapeutic in vivo applications.

Labeled Antibodies

The present invention further provides the above-described antibodies in detectably labeled form. Antibodies can be detectably labelled through the use of radio isotopes, affinity labels (such as biotin, avidin, etc.), enzymatic labels (such as horseradish peroxidase, alkaline phosphatase, etc.) fluorescent labels (such as FITC or rhodamine, etc.), paramagnetic atoms, etc. Procedures for accomplishing such labeling are well-known in the art, for example see (Sternberger et al , J. Histochem. Cytochem. 18:315 (1970); Bayer et al, Meth. Enzym. 62:308 (1979); Engval et al, Immunol. 109:129 (1972); Goding, J. Immunol. Meth. 13:215 (1976)). The labeled antibodies of the present invention can be used for vitro, in vivo, and in situ diagnostic assays.

Immobilized Antibodies

The foregoing antibodies also may be immobilized on a solid support. Examples of such solid supports include plastics such as polycarbonate, complex carbohydrates such as agarose and sepharose, acrylic resins and such as polyacrylamide and latex beads. Techniques for coupling antibodies to such solid supports are well known in the art (Weiret al, "Handbook of Experimental Immunology" 4th Ed., Blackwell Scientific Publications, Oxford, England, Chapter 10 (1986); Jacoby et al, Meth. Enzym. 34 Academic Press, N.Y. (1974)). The immobilized antibodies of the present invention can be used for in vitro, in vivo, and in situ assays as well as for immunoaffimty purification of the proteins of the present invention.

THERAPEUTIC AND DIAGNOSTIC COMPOSITIONS

The proteins, antibodies and polynucleotides of the present invention can be formulated according to known methods to prepare pharmaceutically useful compositions, whereby these materials, or their functional derivatives, are combined in admixture with a pharmaceutically acceptable carrier vehicle. Suitable vehicles and their formulation, inclusive of other human proteins, e.g., human serum albumin, are described, for example, in Remington's Pharmaceutical Sciences (16th ed., Osol, A., Ed., Mack, Easton PA (1980)). In order to form a pharmaceutically acceptable composition suitable for effective administration, such compositions will contain an effective amount of one or more of the agents of the present invention, together with a suitable amount of carrier vehicle.

Pharmaceutical compositions for use in accordance with the present invention may be formulated in conventional manner using one or more physiologically acceptable carriers or excipients. Thus, the compounds and their physiologically acceptable salts and solvate may be formulated for administration by inhalation or insufflation (either through the mouth or the nose) or oral, buccal, parenteral or rectal administration.

For oral administration, the pharmaceutical compositions may take the form of, for example, tablets or capsules prepared by conventional means with pharmaceutically acceptable excipients such as binding agents (e.g. , pregelatinised maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose); fillers (e.g. , lactose, microcrystalline cellulose or calcium hydrogen phosphate); lubricants (e.g. , magnesium stearate, talc or silica); disintegrants (e.g. , potato starch or sodium starch glycolate); or wetting agents (e.g. , sodium lauryl sulphate). The tablets may be coated by methods well known in the art. Liquid preparations for oral administration may take the form of, for example, solutions, syrups or suspensions, or they maybe presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations may be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents (e.g. , sorbitol syrup, cellulose derivatives or hydrogenated edible fats); emulsifying agents (e.g. , lecithin or acacia); non-aqueous vehicles (e.g. , almond oil, oily esters, ethyl alcohol or fractionated vegetable oils); and preservatives (e.g. , methyl or propyl- p-hydroxybenzoates or sorbic acid). The preparations may also contain buffer salts, flavoring, coloring and sweetening agents as appropriate.

Preparations for oral administration may be suitably formulated to give controlled release of the active compound. For buccal administration the composition may take the form of tablets or lozenges formulated in conventional manner.

For administration by inhalation, the compounds for use according to the present invention are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or a nebuliser, with the use of a suitable propellant, e.g. , dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol the dosage unit may be determined by providing a valve to deliver a metered amount. Capsules and cartridges of, e.g. gelatin for use in an inhaler or insufflator may be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch.

The compounds may be formulated for parenteral administration by injection, e.g. , by bolus injection or continuous infusion. Formulations for injection may be presented in unit dosage form, e.g. , in ampules or in multi-dose containers, with an added preservative. The compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents. Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e.g. , sterile pyrogen-free water, before use.

The compounds may also be formulated in rectal compositions such as suppositories or retention enemas, e.g. , containing conventional suppository bases such as cocoa butter or other glycerides.

In addition to the formulations described previously, the compounds may also be formulated as a depot preparation. Such long acting formulations may be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection. Thus, for example, the compounds may be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt.

The compositions may, if desired, be presented in a pack or dispenser device which may contain one or more unit dosage forms containing the active ingredient. The pack may for example comprise metal or plastic foil, such as a blister pack. The pack or dispenser device may be accompanied by instructions for administration.

RECOMBINANT CONSTRUCTS AND EXPRESSION

The present invention further provides recombinant DNA constructs comprising one or more of the nucleotide sequences of the present invention. The recombinant constructs of the present invention comprise a vector, such as a plasmid or viral vector, into which a DNA or DNA fragment, typically bearing an open reading frame, is inserted, in either orientation.

The gene products encoded by the subject DNAs may be produced by recombinant DNA technology using techniques well known in the art. See, for example, the techniques described in Sambrook et al., 1989, supra, and Ausubel et al., 1989, supra. Alternatively, the DNA sequences may be chemically synthesized using, for example, synthesizers. See, for example, the techniques described in OLIGONUCLEOTIDE SYNTHESIS, 1984, Gait, ed., IRL Press, Oxford, which is incorporated by reference herein in its entirety. They may be assembled from fragments and short oligonucleotide linkers, or from a series of oligonucleotides. The are preferably made by RT-PCR methods. The resulting synthetic gene is capable of being expressed in a recombinant vector.

In some cases the recombinant constructs will be expression vectors, which are capable of expressing the RNA and/or protein products of the encoded DNA(s). Thus, the vector may further comprise regulatory sequences, including for example, a promoter, operably linked to the open reading frame (ORF). The vector may further comprise a selectable marker sequence.

Specific initiation signals may also be required for efficient translation of inserted target gene coding sequences. These signals include the ATG initiation codon and adjacent sequences. In cases where a target DNA includes its own initiation codon and adjacent sequences is inserted into the appropriate expression vector, no additional translation control signals may be needed. However, in cases where only a portion of an ORF is used, exogenous translational control signals, including, perhaps, the ATG initiation codon, must be provided. Furthermore, the initiation codon must be in phase with the reading frame of the desired coding sequence to ensure translation of the entire target. These exogenous translational control signals and initiation codons can be of a variety of origins, both natural and synthetic. The efficiency of expression may be enhanced by the inclusion of appropriate transcription enhancer elements, transcription terminators, etc. (see Bittner et al, Methods in Enzymol. 153:516-544 (1987)). Some appropriate cloning and expression vectors for use with prokaryotic and eukaryotic hosts are described by Sambrook, et al, in Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor, New York (1989), the disclosure of which is hereby incorporated by reference.

If desired, to enhance expression and facilitate proper protein folding, the codon context and codon pairing of the sequence may be optimized for the particular expression organism, as explained by Hatfieldet /., U.S. Patent No. 5,082,767.

The present invention further provides host cells containing at least one of the DNAs of the present invention. The host cell can be virtually any cell for which expression vectors are available. It may be, for example, a higher eukaryotic host cell, such as a mammalian cell, a lower eukaryotic host cell, such as a yeast cell, or the host cell can be a prokaryotic cell, such as a bacterial cell. Introduction of the recombinant construct into the host cell can be effected by calcium phosphate transfection, DEAE, dextran mediated transfection, or electroporation (Davis et al, Basic Methods in Molecular Biology (1986)).

A wide variety of expression systems are available, such as: yeast (e.g. Saccharomyces, Pichia) transformed with recombinant yeast expression vectors containing the target DNA; insect cell systems infected with recombinant virus expression vectors (e.g. , baculovirus) containing the target DNA sequences; plant cell systems infected with recombinant virus expression vectors (e.g. , cauliflower mosaic virus, CaMV; tobacco mosaic virus, TMV) or transformed with recombinant plasmid expression vectors (e.g. Ti plasmid) containing target DNA coding sequences; or mammalian cell systems (e.g. COS, CHO, BHK, 293, 3T3) harboring recombinant expression constructs containing promoters derived from the genome of mammalian cells (e.g. , metallothionein promoter) or from mammalian viruses (e.g. , the adenovirus late promoter; the vaccinia virus 7.5K promoter).

Depending on the system chosen, the resulting product may differ. For example, proteins expressed in most bacterial cultures, e.g. , E. coli, will be free of glycosylation modifications; polypeptides or proteins expressed in yeast will have a glycosylation pattern different from that expressed in mammalian cells.

Vectors

Generally, recombinant expression vectors will include origins of replication and selectable markers permitting selection of the host cell, e.g. , the ampicillin resistance gene of E. coli and S. cerevisiae TRP1 gene, and a promoter derived from a highly -expressed gene to direct transcription of a downstream structural sequence. Such promoters can be derived from operons encoding glycolytic enzymes such as 3 -phosphogly cerate kinase (PGK), α-factor, acid phosphatase, or heat shock proteins, among others. The heterologous structural sequence is assembled in appropriate phase with translation initiation and termination sequence, and in one aspect of the invention, a leader sequence capable of directing secretion of translated protein into the periplasmic space or extracellular medium. Optionally, the heterologous sequence can encode a fusion protein including an N-terminal or C-terminal identification peptide imparting desired characteristics, e.g. , stabilization or simplified purification of expressed recombinant product. Bacterial Expression

Useful expression vectors for bacterial use are constructed by inserting a structural DNA sequence encoding a desired protein together with suitable translation initiation and termination signals in operable reading phase with a functional promoter. The vector will comprise one or more phenotypic selectable markers and an origin of replication to ensure maintenance of the vector and, if desirable, to provide amplification within the host. Suitable prokaryotic hosts for transformation include E. coli, Bacillus subtilis, Salmonella typhimurium and various species within the genera Pseudomonas, Streptomyces, and Staphylococcus, although others may, also be employed as a matter of choice.

Bacterial vectors may be, for example, bacteriophage-, plasmid- or cosmid-based. These vectors can comprise a selectable marker and bacterial origin of replication derived from commercially available plasmids typically containing elements of the well known cloning vector pBR322 (ATCC 37017). Such commercial vectors include, for example, GEM 1 (Promega Biotec, Madison, WI, USA), pBs, phagescript, PsiX174, pBluescript SK, pBs KS, pNH8a, pNHlόa, pNH18a, pNH46a (Stratagene); pTrc99A, pKK223-3, pKK233-3, pKK232-8, pDR540, and pRIT5 (Pharmacia).

These "backbone" sections are combined with an appropriate promoter and the structural sequence to be expressed. Bacterial promoters include lac, T3, T7, lambda P_R or P_L, tip, and ara.

Following transformation of a suitable host strain and growth of the host strain to an appropriate cell density, the selected promoter is derepressed/ induced by appropriate means (e.g., temperature shift or chemical induction) and cells are cultured for an additional period. Cells are typically harvested by centrifugation, disrupted by physical or chemical means, and the resulting crude extract retained for further purification.

In bacterial systems, a number of expression vectors may be advantageously selected depending upon the use intended for the protein being expressed. For example, when a large quantity of such a protein is to be produced, for the generation of antibodies or to screen peptide libraries, for example, vectors which direct the expression of high levels of fusion protein products that are readily purified may be desirable. Such vectors include, but are not limited, to the E. coli expression vector pUR278 (Ruther et al., 1983, EMBO J. 2:1791), in which the coding sequence may be ligated into the vector in frame with the lac Z coding region so that a fiision protein is produced; pIN vectors (Inouye et al 1985, Nucleic Acids Res. 13:3101-3109; Van Heeke et α/. , 1989, 7. Biol Chem. 264:5503-5509); pET vectors, Studier et al , Methods in Enzymology 185: 60-89 (Academic Press 1990); and the like.

Moreover, pGEX vectors may be used to express foreign polypeptides as fusion proteins with glutathione S-transferase (GST). In general, such fusion proteins are soluble and easily can be purified from lysed cells by adsorption to glutathione-agarose beads followed by elution in the presence of free glutathione. The pGEX vectors are designed to include thrombin or factor Xa protease cleavage sites so that the cloned target gene protein can be released from the GST moiety.

In a one embodiment, full length cDNA sequences are appended with in-frame BamHl sites at the amino terminus and EcoRI sites at the carboxyl terminus using standard PCR methodologies (Innis et al., 1990, supra) and ligated into the pGEX-2TK vector (Pharmacia, Uppsala, Sweden). The resulting cDNA construct contains a kinase recognition site at the amino terminus for radioactive labeling and glutathione S-transferase sequences at the carboxyl terminus for affinity purification (Nilsson, et al. 1985, EMBO J. 4: 1075; Zabeau and Stanley, 1982, EMBO J. 1: 1217.

Eukaryotic Expression

Various mammalian cell culture systems can also be employed to express recombinant protein. Examples of mammalian expression systems include the COS-7 lines of monkey kidney fibroblasts, described by Gluzman, Cell 23:175 (1981), and other cell lines capable of expressing a compatible vector, for example, the C127, 3T3, CHO, HeLa and BHK cell lines. Mammalian expression vectors will comprise an origin of replication, a suitable promoter and enhancer, and also any necessary ribosome binding sites, polyadenylation site, splice donor and acceptor sites, transcriptional termination sequences, and 5' flanking nontranscribed sequences. DNA sequences derived from the SV40 viral genome, for example, SV40 origin, early promoter, enhancer, splice, and polyadenylation sites may be used to provide the required nontranscribed genetic elements.

Mammalian promoters include CMV immediate early, HSV thymidine kinase, early and late SV40, LTRs from retrovirus, and mouse metallothionein-I. Exemplary mammalian vectors include pWLneo, pSV2cat, pOG44, pXTl, pSG (Stratagene) pSVK3, pBPV, pMSG, and pSVL (Pharmacia). Selectable markers include CAT (chloramphenicol transferase).

In mammalian host cells, a number of viral-based expression systems may be utilized. In cases where an adenovirus is used as an expression vector, the coding sequence of interest may be ligated to an adenovirus transcription/translation control complex, e.g. , the late promoter and tripartite leader sequence. This chimeric gene may then be inserted in the adenovirus genome by in vitro or in vivo recombination. Insertion in a non-essential region of the viral genome (e.g. , region El or E3) will result in a recombinant virus that is viable and capable of expressing a target protein in infected hosts. (E.g. , See Logan et al. , 1984, Proc. Natl Acad. Sci. USA 81:3655-3659).

In one embodiment, cDNA sequences encoding the full-length open reading frames are ligated into pCMVβ replacing the β-galactosidase gene such that cDNA expression is driven by the CMV promoter (Alam, 1990, Anal. Biochem. 188: 245-254; MacGregor et al , 1989, Nucl Acids Res. 17: 2365; Norton et al 1985, Mol. Cell Biol. 5: 281).

In addition, a host cell strain may be chosen which modulates the expression of the inserted sequences, or modifies and processes the gene product in the specific fashion desired. Such modifications (e.g. , glycosylation) and processing (e.g. , cleavage) of protein products may be important for the function of the protein. Different host cells have characteristic and specific mechanisms for the post-translational processing and modification of proteins.

Appropriate cell lines or host systems can be chosen to ensure the correct modification and processing of the foreign protein expressed. To this end, eukaryotic host cells which possess the cellular machinery for proper processing of the primary transcript, glycosylation, and phosphorylation of the gene product may be used. Such mammalian host cells include but are not limited to CHO, VERO, BHK, HeLa, COS, MDCK, 293, 3T3, WI38, etc.

For long-term, high-yield production of recombinant proteins in eukaryotic cells, stable expression is preferred. Rather than using expression vectors which contain viral origins of replication, host cells can be transformed with DNA controlled by appropriate expression control elements (e.g. , promoter, enhancer, sequences, transcription terminators, polyadenylation sites, etc.), and a selectable marker.

Following the introduction of the foreign DNA, engineered cells may be allowed to grow for 1-2 days in an enriched media, and then are switched to a selective media. The selectable marker in the recombinant plasmid confers resistance to the selection and allows cells to stably integrate the plasmid into their chromosomes and grow to form foci which in turn can be cloned and expanded into cell lines. This method may advantageously be used to engineer cell lines which express the target protein. Such engineered cell lines may be particularly useful in screening and evaluation of compounds that affect the endogenous activity of the protein.

A number of selection systems may be used, including but not limited to the herpes simplex virus thymidine kinase (Wigler, et al , Cell 11:223 (1977)), hypoxanthine-guanine phosphoribosyltransferase(Szybalskaet α/., Proc. Natl. Acad. Sci. USA 48:2026 (1962)), and adenine phosphoribosyltransferase(Lowy, et al , Cell 22:817 (1980)) genes can be employed in tk^", hgprt^" or aprf cells, respectively. Also, antimetabolite resistance can be used as the basis of selection for dhfr, which confers resistance to methotrexate (Wigler, et al. , Proc. Natl. Acad, Sci. USA 77:3567 (1980)); O'Hare, et al , 1981, Proc. Natl. Acad. Sci. USA 78:1527); gpt, which confers resistance to mycophenolic acid (Mulligan et al , Proc. Natl. Acad. Sci. USA 78:2072 (1981)); neo, which confers resistance to the aminoglycoside G-418 (Colberre-Garapin, et al. , 1981, J. Mol. Biol 150: 1); and hydro, which confers resistance to hygromycin (Santerre, et al. , 1984, Gene 30: 147) genes.

An alternative fusion protein system allows for the ready purificationof non-denatured fusion proteins expressed in human cell lines (Janknecht, et al. , Proc. Natl Acad. Sci. USA 88: 8972-8976 (1991)). In this system, the gene of interest is subcloned into a vaccinia-based plasmid such that the gene's open reading frame is translationally fused to an amino-terminal tag consisting of six histidine residues. Extracts from cells infected with recombinant vaccinia virus are loaded onto N?⁺ nitriloacetic acid-agarose columns and histidine-tagged proteins are selectively eluted with imidazole-containing buffers.

In an insect system, Autographa californica nuclear polyhedrosis virus (AcNPV) is used as a vector to express foreign genes. The virus grows in Spodoptera frugiperda cells. The target coding sequence may be cloned individually into non-essential regions (for example the polyhedrin gene) of the virus and placed under control of an AcNPV promoter (for example the polyhedrin promoter). Successful insertion of a target gene coding sequence will result in inactivation of the polyhedrin gene and production of non-occluded recombinant virus (i.e., virus lacking the proteinaceous coat coded for by the polyhedrin gene). These recombinant viruses are then used to infect Spodoptera frugiperda cells in which the inserted gene is expressed. (E.g. , see Smith et al., 1983, J. Virol. 46: 584; Smith, U.S. Patent No. 4,215,051). While the present proteins can be expressed in recombinant systems, as described above, cell-free translation systems can also be employed to produce such proteins using RNAs derived from the DNA constructs of the present invention.

Purification of Recombinant Proteins

Recombinant proteins produced may be isolated by host cell lysis. This may be followed by one or more salting-out, aqueous ion exchange or size exclusion chromatography steps. Finally, high performance liquid chromatography (HPLC) can be employed for final purification steps. Microbial cells employed in expression of proteins can be disrupted by any convenient method, including freeze-thaw cycling, sonication, mechanical disruption, or use of cell lysing agents, like lysozyme and chelators.

If inclusion bodies are formed in bacterial systems, they may be extracted from cell pellets using, for example, detergents, reducing agents, salts, urea, guanidinium chloride and extremes of pH (e.g. <4 or > 10). If denaturation occurs, protein refolding steps (e.g. , dialysis) can be used, as necessary, in completing configuration of the mature protein. If disulfide bridges are present in the native protein, they may be reoxidized using known methods.

By way of specific non-limiting example, the recombinant bacterial cells, for example E. coli, are grown in any of a number of suitable media, for example LB, and the expression of the recombinant protein induced by adding IPTG (e.g. , lac operator-promoter) to the media or switching incubation to a higher temperature (e.g. , λ cl⁸⁵⁷). After culturing the bacteria for a further period of between 2 and 24 hours, the cells are collected by centrifugation and washed to remove residual media. The bacterial cells are then lysed, for example, by disruption in a cell homogenizer and centrifuged to separate the cell membranes from the soluble cell components. If the protein aggregates into inclusion bodies, this centrifugation can be performed under conditions whereby the dense inclusion bodies are selectively enriched by incorporation of sugars such as sucrose into the buffer and centrifugation at a selective speed. The inclusion bodies can then be washed in any of several solutions to remove some of the contaminating host proteins, then solubilized in solutions containing high concentrations of urea (e.g. 8M) or chaotropic agents such as guanidinium hydrochloride in the presence of reducing agents such as β-mercaptoethanolor DTT (dithiothreitol).

At this stage it may be advantageous to incubate the protein for several hours under conditions suitable for the protein to undergo a refolding process into a conformation which more closely resembles that of the native protein. Such conditions generally include low protein concentrations less than 500 μg/ml), low levels of reducing agent, concentrations of urea less than 2 M and often the presence of reagents such as a mixmre of reduced and oxidized glutathione which facilitate the interchange of disulphide bonds within the protein molecule. The refolding process can be monitored, for example, by SDS-PAGE or with antibodies which are specific for the native molecule. Following refolding, the protein can then be purified further and separated from the refolding mixture by chromatography on any of several supports including ion exchange resins, gel permeation resins or on a variety of affinity columns.

Labeling Proteins

When used as a component in assay systems such as those described, below, the target protein may be labeled, either directly or indirectly, to facilitate detection of the present res- like molecules either in vitro or in vivo. Any of a variety of suitable labeling systems may be used including but not limited to radioisotopes such as ¹²⁵I; enzyme labeling systems that generate a detectable colorimetric signal or light when exposed to substrate; and fluorescent labels.

Where recombinant DNA technology is used for protein production the, it may be advantageous to engineer fusion proteins that can facilitate labeling, immobilization and/or detection. These fusion proteins may, for example, add amino acids which facilitate further chemical modification. They also may add a functional moiety, such as an enzyme, which directly facilitates detection.

TRANSGENIC ANIMALS

The invention further contemplates animal models for studying the function of the present molecules and for overproducing the protein products. The disclosed DNA sequences may be used in conjunction with techniques for producing transgenic animals that are well known to those of skill in the art. To prepare transgenic animals, target gene sequences may for example be introduced into, and overexpressed in, the genome of the animal of interest, or, if endogenous target gene sequences are present, they may either be overexpressed or, alternatively, be disrupted in order to underexpress or inactivate target gene expression, such as described for the disruption of apoE in mice (Plum et al , Cell 71 : 343-353 (1992)).

In order to overexpress a target gene sequence, the coding portion of the target gene sequence may be ligated to a regulatory sequence which is capable of driving gene expression in the animal and cell type of interest. Such regulatory regions will be well known to those of skill in the art, and may be utilized in the absence of undue experimentation.

For underexpression of an endogenous target gene sequence, such a sequence may be isolated and engineered such that when reintroduced into the genome of the animal of interest, the endogenous target gene alleles will be inactivated. Preferably, the engineered target gene sequence is introduced via gene targeting such that the endogenous target sequence is disrupted upon integration of the engineered target gene sequence into the animal ' s genome .

Animals of any species, including, but not limited to, mice, rats, rabbits, guinea pigs, pigs, micro-pigs, goats, and non-human primates, e.g. , baboons, monkeys, and chimpanzees may be used to generate cardiovascular disease animal models. Goats, cows and sheep are particularly preferred for producing protein in vivo.

Any technique known in the art may be used to introduce a target gene transgene into animals to produce the founder lines of transgenic animals. Such techniques include, but are not limited to pronuclear microinjection (Hoppe et al , U.S. Pat. No. 4,873,191 (1989)); retrovirus mediated gene transfer into germ lines (Van der Putten et al. , Proc. Natl. Acad. Sci., USA 82:6148-6152 (1985)); gene targeting in embryonic stem cells (Thompson et al , Cell 56:313-321 (1989)); electroporation of embryos (Lo, Mol. Cell. Biol. 3:1803-1814 (1983)); and sperm-mediated gene transfer (Lavitrano et al , Cell 57:717-723 (1989)); etc. For a review of such techniques, see Gordon, Transgenic Animals, Intl. Rev. Cytol. 115:171- 229 (1989).

The present invention provides for transgenic animals that carry the transgene in all their cells, as well as animals which carry the transgene in some, but not all their cells, i.e. , mosaic animals. The transgene may be integrated as a single transgene or in concatamers, e.g. , head-to-head tandems or head-to-tail tandems. The transgene may also be selectively introduced into and activated in a particular cell type by following, for example, the teaching of Lasko et al. (Lasko et al , Proc. Natl Acad. Sci. USA 89:3232-6236 (1992)). The regulatory sequences required for such a cell-type specific activation will depend upon the particular cell type of interest, and will be apparent to those of skill in the art. When it is desired that the target gene be integrated into the chromosomal site of the endogenous target gene, gene targeting is preferred. Briefly, when such a technique is to be utilized, vectors containing some nucleotide sequences homologous to the endogenous target gene of interest are designed for the purpose of integrating, via homologous recombination with chromosomal sequences, into and disrupting the function of the nucleotide sequence of the endogenous target gene.

The transgene may also be selectively introduced into a particular cell type, thus inactivating the endogenous gene of interest in only that cell type, by following, for example, the teaching of Gu et al. Science 265: 103-106 (1994)). The regulatory sequences required for such a cell-type specific inactivation will depend upon the particular cell type of interest, and will be apparent to those of skill in the art.

Once transgenic animals have been generated, the expression of the recombinant target gene and protein may be assayed utilizing standard techniques. Initial screening may be accomplished by Southern blot analysis or PCR techniques to analyze animal tissues to assay whether integration of the transgene has taken place. The level of mRNA expression of the transgene in the tissues of the transgenic animals may also be assessed using techniques which include but are not limited to Northern blot analysis of tissue samples obtained from the animal, in situ hybridization analysis, and RT-PCR. Samples of target gene-expressing tissue, may also be evaluated immunocytochemically using antibodies specific for the target gene transgene gene product of interest.

The transgenic animals that express target gene mRNA or target gene transgene peptide (detected immunocytochemically, using antibodies directed against the target gene product's epitopes) at easily detectable levels should then be further evaluated to identify those animals which display characteristic increased susceptibility to carcinogenesis. Additionally, specific cell types within the transgenic animals may be analyzed and assayed in vitro for cellular phenotypes characteristic of mutant phenotype.

Once target gene transgenic founder animals are produced, they may be bred, inbred, outbred, or crossbred to produce colonies of the particular animal. Examples of such breeding strategies include but are not limited to: outbreeding of founder animals with more than one integration site in order to establish separate lines; inbreeding of separate lines in order to produce compound target gene transgenics that express the target gene transgene of interest at higher levels because of the effects of additive expression of each target gene transgene; crossing of heterozygous transgenic animals to produce animals homozygous for a given integration site in order both to augment expression and eliminate the possible need for screening of animals by DNA analysis; crossing of separate homozygous lines to produce compound heterozygous or homozygous lines; breeding animals to different inbred genetic backgrounds so as to examine effects of modifying alleles on expression of the target gene transgene and the possible development of carcinogenesis. One such approach is to cross the target gene transgenic founder animals with a wild type strain to produce an Fl generation that exhibits increased susceptibility to carcinogenesis. The Fl generation may then be inbred in order to develop a homozygous line, if it is found that homozygous target gene transgenic animals are viable.

Methods of generating "knockout" mice using homologous recombination in embryonic stem cells are well known in the art. Suitable methods are described, for example, in Mansour et al , Nature, 336:348 (1988); Zijlstra et al , Nature, 342:435 (1989) and 344:742 (1990); and Hasty et al , Nature, 350:243 (1991). This genomic DNA can be obtained by conventional methods using the cDNA sequence as a probe in a commercially- available genomic DNA library.

Briefly, a genomic fragment is cleaved with a restriction endonuclease and a heterologous cassette containing a neomycin-resistancegene is inserted at the cleavage site. A suitable cassette is the GTI-II neo cassette described by Lufkin et al , Cell 66:1105 (1991). The modified genomic fragment is cloned into a suitable targeting vector that is introduced into murine embryonic stem cells by electroporation. Cells that have undergone homologous recombination (and hence disruption of the gene) are selected by resistance to G418, and used to generate chimeric mice using well known methods. See Lufkin et al, supra. Traditional breeding methods then can be used to generate mice that are homozygous for the disrupted gene.

The phenotype of mice that are homozygous for the mutation then can be studied to provide insights into the role of the protein in, for example, carcinogenesis. These mice also can be used as models for developing new treatments for cancers. If this mutation is lethal in homozygous mice (for example during embryogenesis) heterozygous mice, which express only half the amount of the protein can also be studied.

GENE THERAPY APPLICATIONS

When mutations in the inventive protein, or in the elements controlling expression of that protein, are found to be associated with a malignant phenotype, control of cellular proliferation can be restored by gene therapy methods. For example, overexpression of the protein can be counteracted by concurrent expression of an antisense molecule that binds to and inhibits expression of the mRNA encoding the protein. Alternatively, overexpression can be inhibited in an analogous manner using a ribozyme that cleaves the mRNA. In another embodiment, where expression of a mutated protein induces the malignant phenotype, concomitant expression of the non-mutated molecule via introduction of an exogenous gene may be used. Methods of using antisense and ribozyme technology to control gene expression, or of gene therapy methods for expression of an exogenous gene in this manner are well known in the art.

Each of these methods requires a system for introducing a vector into the cells containing the mutated gene. The vector encodes either an antisense or ribozyme transcript of the inventive protein. The construction of a suitable vector can be achieved by any of the methods well-known in the art for the insertion of exogenous DNA into a vector. See, e.g. , Sambrook et al, Molecular Cloning (Cold Spring Harbor Press 2d ed. 1989), which is incorporated herein by reference. In addition, the prior art teaches various methods of introducing exogenous genes into cells in vivo. See Rosenberg et al. , Science 242: 1575-1578 (1988) and Wolff et al , PNAS 86:9011-9014 (1989), which are incorporated herein by reference. The routes of delivery include systemic admimstration and admimstration in situ. Well-known techniques include systemic administration with cationic liposomes, and admimstration in situ with viral vectors. Any one of the gene delivery methodologies described in the prior art is suitable for the introduction of a recombinant vector containing an inventive gene according to the invention into a MTX-resistant, transport-deficient cancer cell. A listing of present-day vectors suitable for the purpose of this invention is set forth in Hodgson, Bio /Technology 13: 222 (1995), which is incorporated by reference.

For example, liposome-mediated gene transfer is a suitable method for the introduction of a recombinant vector containing an inventive gene according to the invention into a MTX-resistant, transport-deficient cancer cell. The use of a cationic liposome, such as DC-Chol/DOPE liposome, has been widely documented as an appropriate vehicle to deliver DNA to a wide range of tissues through intravenous injection of DNA/cationic liposome complexes. See Caplen et al , Nature Med. 1:39-46 (1995) and Zhu et al, Science 261:209- 211 (1993), which are herein incorporated by reference. Liposomes transfer genes to the target cells by fusing with the plasma membrane. The entry process is relatively efficient, but once inside the cell, the liposome-DNA complex has no inherent mechanism to deliver the DNA to the nucleus. As such, the most of the lipid and DNA gets shunted to cytoplasmic waste systems and destroyed. The obvious advantage of liposomes as a gene therapy vector is that liposomes contain no proteins, which thus minimizes the potential of host immune responses.

As another example, viral vector-mediated gene transfer is also a suitable method for the introduction of the vector into a target cell. Appropriate viral vectors include adenovirus vectors and adeno-associated virus vectors, retrovirus vectors and herpesvirus vectors.

Adenoviruses are linear, double stranded DNA viruses complexed with core proteins and surrounded by capsid proteins. The common serotypes 2 and 5, which are not associated with any human malignancies, are typically the base vectors. By deleting parts of the virus genome and inserting the desired gene under the control of a constitutive viral promoter, the virus becomes a replication deficient vector capable of transferring the exogenous DNA to differentiated, non-proliferating cells. To enter cells, the adenovirus fibre interacts with specific receptors on the cell surface, and the adenovirus surface proteins interact with the cell surface integrins. The virus penton-cell integrin interaction provides the signal that brings the exogenous gene-containing virus into a cytoplasmic endosome. The adenovirus breaks out of the endosome and moves to the nucleus, the viral capsid falls apart, and the exogenous DNA enters the cell nucleus where it functions, in an epichromosomal fashion, to express the exogenous gene. Detailed discussions of the use of adenoviral vectors for gene therapy can be found in Berkner, Biotechniques 6:616-629 (1988) and Trapnell, Advanced Drug Delivery Rev. 12: 185-199 (1993), which are herein incorporated by reference. Adenovirus-derived vectors, particularly non-replicative adenovirus vectors, are characterized by their ability to accommodate exogenous DNA of 7.5 kB, relative stability, wide host range, low pathogenicity in man, and high titers (10⁴ to 10⁵ plaque forming units per cell). See Stratford- Perricaudet et al , PNAS 89:2581 (1992). Adeno-associated virus (AAV) vectors also can be used for the present invention. AAV is a linear single-stranded DNA parvovirus that is endogenous to many mammalian species. AAV has a broad host range despite the limitation that AAV is a defective parvovirus which is dependent totally on either adenovirus or herpesvirus for its reproduction in vivo. The use of AAV as a vector for the introduction into target cells of exogenous DNA is well-known in the art. See, e.g. , Lebkowski et al , Mole. & Cell. Biol 8:3988 (1988), which is incorporated herein by reference. In these vectors, the capsid gene of AAV is replaced by a desired DNA fragment, and transcomplementation of the deleted capsid function is used to create a recombinant virus stock. Upon infection the recombinant virus uncoats in the nucleus and integrates into the host genome.

Another suitable virus-based gene delivery mechanism is retroviral vector-mediated gene transfer. In general, retroviral vectors are well-known in the art. See Breakfield et al. , Mole. Neuro. Biol 1:339 (1987) and Shih et al , in Vaccines 85: 177 (Cold Spring Harbor Press 1985). A variety of retroviral vectors and retroviral vector-producing cell lines can be used for the present invention. Appropriate retroviral vectors include Moloney Murine Leukemia Virus, spleen necrosis virus, and vectors derived from retroviruses such as Rous Sarcoma Virus, Harvey Sarcoma Virus, avian leukosis virus, human immunodeficiency virus, myeloproliferative sarcoma virus, and mammary tumor virus. These vectors include replication-competent and replication-defective retroviral vectors. In addition, amphotropic and xenotropic retroviral vectors can be used. In carrying out the invention, retroviral vectors can be introduced to a tumor directly or in the form of free retroviral vector producing-cell lines. Suitable producer cells include fibroblasts, neurons, glial cells, keratinocytes, hepatocytes, connective tissue cells, ependymal cells, chromaffin cells. See Wolff et al, PNAS 84:3344 (1989).

Retroviral vectors generally are constructed such that the majority of its structural genes are deleted or replaced by exogenous DNA of interest, and such that the likelihood is reduced that viral proteins will be expressed. See Bender et al, J. Virol. 61: 1639 (1987) and Armento et al, J. Virol 61:1647 (1987), which are herein incorporated by reference. To facilitate expression of the antisense or ribozyme molecule, of the inventive protein, a retroviral vector employed in the present invention must integrate into the genome of the host cell genome, an event which occurs only in mitotically active cells. The necessity for host cell replication effectively limits retroviral gene expression to tumor cells, which are highly replicative, and to a few normal tissues. The normal tissue cells theoretically most likely to be transduced by a retroviral vector, therefore, are the endothelial cells that line the blood vessels that supply blood to the tumor. In addition, it is also possible that a retroviral vector would integrate into white blood cells both in the tumor or in the blood circulating through the tumor.

The spread of retroviral vector to normal tissues, however, is limited. The local administration to a tumor of a retroviral vector or retroviral vector producing cells will restrict vector propagation to the local region of the tumor, minimizing transduction, integration, expression and subsequent cytotoxic effect on surrounding cells that are mitotically active.

Both replicatively deficient and replicatively competent retroviral vectors can be used in the invention, subject to their respective advantages and disadvantages. For instance, for mmors that have spread regionally, such as lung cancers, the direct injection of cell lines that produce replication-deficient vectors may not deliver the vector to a large enough area to completely eradicate the tumor, since the vector will be released only form the original producer cells and their progeny, and diffusion is limited. Similar constraints apply to the application of replication deficient vectors to tumors that grow slowly, such as human breast cancers which typically have doubling times of 30 days versus the 24 hours common among human gliomas. The much shortened survival-time of the producer cells, probably no more than 7-14 days in the absence of immunosuppression, limits to only a portion of their replicative cycle the exposure of the tumor cells to the retroviral vector.

The use of replication-defective retroviruses for treating mmors requires producer cells and is limited because each replication-defective retrovirus particle can enter only a single cell and cannot productively infect others thereafter. Because these replication- defective retroviruses cannot spread to other tumor cells, they would be unable to completely penetrate a deep, multilayered tumor in vivo. See Markert et al, Neurosurg. 77: 590 (1992). The injection of replication-competent retroviral vector particles or a cell line that produces a replication-competent retroviral vector virus may prove to be a more effective therapeutic because a replication competent retroviral vector will establish a productive infection that will transduce cells as long as it persists. Moreover, replicatively competent retroviral vectors may follow the tumor as it metastasizes, carried along and propagated by transduced tumor cells. The risks for complications are greater, with replicatively competent vectors, however. Such vectors may pose a greater risk then replicatively deficient vectors of transducing normal tissues, for instance. The risks of undesired vector propagation for each type of cancer and affected body area can be weighed against the advantages in the situation of replicatively competent verses replicatively deficient retroviral vector to determine an optimum treatment.

Both amphotropic and xenotropic retroviral vectors may be used in the invention. Amphotropic viruses have a very broad host range that includes most or all mammalian cells, as is well known to the art. Xenotropic viruses can infect all mammalian cell cells except mouse cells. Thus, amphotropic and xenotropic retroviruses from many species, including cows, sheep, pigs, dogs, cats, rats, and mice, inter alia can be used to provide retroviral vectors in accordance with the invention, provided the vectors can transfer genes into proliferating human cells in vivo.

Clinical trials employing retroviral vector therapy treatment of cancer have been approved in the United States. See Culver, Clin. Chem. 40: 510 (1994). Retroviral vector- containing cells have been implanted into brain tumors growing in human patients. See Oldfield et al, Hum. Gene Ther. 4: 39 (1993). These retroviral vectors carried the HSV-1 thymidine kinase (HSV-tk) gene into the surrounding brain tumor cells, which conferred sensitivity of the mmor cells to the antiviral drug ganciclovir. Some of the limitations of current retroviral based cancer therapy, as described by Oldfield are: (1) the low titer of virus produced, (2) virus spread is limited to the region surrounding the producer cell implant, (3) possible immune response to the producer cell line, (4) possible insertional mutagenesis and transformation of retroviral infected cells, (5) only a single treatment regimen of pro-drug, ganciclovir, is possible because the "suicide" product kills retrovirally infected cells and producer cells and (6) the bystander effect is limited to cells in direct contact with retrovirally transformed cells. See Bi et al. , Human Gene Therapy 4: 725 (1993).

Yet another suitable virus-based gene delivery mechanism is herpesvirus vector- mediated gene transfer. While much less is known about the use of herpesvirus vectors, replication-competent HSV-1 viral vectors have been described in the context of antitumor therapy. See Martuza et al, Science 252: 854 (1991), which is incorporated herein by reference. DIAGNOSTIC METHODS

The present invention also contemplates, for certain molecules described below, methods for diagnosis of human disease. In particular, patients can be screened for the occurrence of cancers, or likelihood of occurrence of cancers, associated with mutations in the encoded protein. DNA from tumor tissue obtained from patients suffering from cancer can be isolated and the gene encoding the protein can be sequenced. By examining a number of patients in this manner, mutations in the gene that are associated with a malignant cellular phenotype can be identified. In addition, correlation of the nature of the observed mutations with subsequent observed clinical outcomes allows development of prognostic model for the predicted outcome in a particular patient.

Screening for mutations conveniently can be carried out at the DNA level by use of PCR, although the skilled artisan will be aware that many other well known methods are available for the screening. PCR primers can be selected that flank known mutation sites, and the PCR products can be sequenced to detect the occurrence of the mutation. Alternatively, the 3 ' residue of one PCR primer can be selected to be a match only for the residue found in the unmutated gene. If the gene is mutated, there will be a mismatch at the 3' end of the primer, and primer extension cannot occur, and no PCR product will be obtained. Alternatively, primer mixtures can be used where the 3' residue of one primer is any nucleotide other than the nonmutated residue. Observation of a PCR product then indicates that a mutation has occurred. Other methods of using, for example, oligonucleotide probes to screen for mutations are described, or example, in U.S. Patent No. 4,871,838, which is herein incorporated by reference in its entirety.

Alternatively, antibodies can be generated that selectively bind either mutated or non- mutated protein. The antibodies then can be used to screen tissue samples for occurrence of mutations in a manner analogous to the DNA-based methods described supra.

The diagnostic methods described above can be used not only for diagnosis and for prognosis of existing disease, but may also be used to predict the likelihood of the future occurrence of disease. For example, clinically healthy patients can be screened for mutations in the inventive molecule that correlate with later disease onset. Such mutations may be observed in the heterozygous state in healthy individuals. In such cases a single mutation event can effectively disable proper functioning of the gene and induce a transformed or malignant phenotype. This screening also may be carried out prenatally or neonatally. DNA molecules according to the invention also are well suited for use in so-called "gene chip" diagnostic applications. Such applications have been developed by, inter alia, Synteni and Affymetrix. Briefly, all or part of the DNA molecules of the invention can be used either as a probe to screen a polynucleotide array on a "gene chip," or they may be immobilized on the chip itself and used to identify other polynucleotides via hybridization to the surface of the chip. In this manner, for example, related genes can be identified, or expression patterns of the gene in various tissues can be simultaneously studied. Such gene chips have particular application for diagnosis of disease, or in forensic analysis to detect the presence or absence of an analyte. Suitable chip technology is described for example, in Wodicka et al , Nature Biotechnology, 15: 1359 (1997) which is hereby incorporated by reference in its entirety, and references cited therein.

PROTEIN-PROTEIN INTERACTIONS

Due to their similarity to certain known proteins, it is anticipated that some of the inventive protein molecules will interact with another class of cellular proteins. This is particularly true of those molecule containing leucine zipper motifs.

Any method suitable for detecting protein-protein interactions can be employed for identifying interacting targets. Among the traditional methods which can be employed are co- immunoprecipitation, crosslinking and co-purification through gradients or chromatographic columns. Utilizing procedures such as these allows for the identification of GAP gene products. Once identified, a GAP protein can be used, in conjunction with standard techniques, to identify its corresponding pathway gene. For example, at least a portion of the amino acid sequence of the pathway gene product can be ascertained using techniques well known to those of skill in the art, such as via the Edman degradation technique (see, e.g.. Creighton, 1983, PROTEINS: STRUCTURES AND MOLECULAR PRINCIPLES, W.H. Freeman & Co. , N. Y. , pp.34-49). The amino acid sequence obtained can be used as a guide for the generation of oligonucleotide mixtures that can be used to screen for pathway gene sequences. Screening can be accomplished, for example, by standard hybridization or PCR techniques. Techniques for the generation of oligonucleotide mixtures and for screening are well-known. (See e.g. , Ausubel, supra, and PCR PROTOCOLS: A GUIDE TO METHODS AND APPLICATIONS, 1990, Innis et al. , eds. Academic Press, Inc. , New York). Additionally, methods can be employed which result in the simultaneous identification of interacting target genes. One method which detects protein interactions in vivo, the two- hybrid system, is described in detail for illustration purposes only and not by way of limitation. One version of this system has been described (Chien et al. , Proc. Natl Acad. Sci. USA, 88: 9578-9582 (1991)) and is commercially available from Clontech (Palo Alto, CA).

Briefly, utilizing such a system, plasmids are constructed that encode two hybrid proteins: one consists of the DNA-binding domain of a transcription activator protein fused to a known protein, in this case an inventive protein, and the other contains the activator protein's activation domain fused to an unknown protein (a putative GAP, for instance) that is encoded by a cDNA which has been recombined into this plasmid as part of a cDNA library. The plasmids are transformed into a strain of the yeast Saccharomyces cerevisiae that contains a reporter gene (e.g., lacZ) whose regulatory region contains the transcription activator's binding sites. Either hybrid protein alone cannot activate transcription of the reporter gene, the DNA-binding domain hybrid cannot because it does not provide activation function, and the activation domain hybrid cannot because it cannot localize to the activator's binding sites. Interaction of the two hybrid proteins reconstitutes the functional activator protein and results in expression of the reporter gene, which is detected by an assay for the reporter gene product.

The two-hybrid system or related methodology can be used to screen activation domain libraries for proteins that interact with a known "bait" gene product. By way of example, and not by way of limitation, gene products known to be involved in TH cell subpopulation-related disorders and/or differentiation, maintenance, and/or effector function of the subpopulations can be used as the bait gene products. Total genomic or cDNA sequences are fused to the DNA encoding on activation domain. This library and a plasmid encoding a hybrid of the bait gene product fused to the DNA-binding domain are cotransformed into a yeast reporter strain, and the resulting transformants are screened for those that express the reporter gene. For example, and not by way of limitation, the bait gene can be cloned into a vector such that it is translationally fused to the DNA encoding the DNA- binding domain of the GAL4 protein. These colonies are purified and the library plasmids responsible for reporter gene expression are isolated. DNA sequencing is then used to identify the proteins encoded by the library plasmids. The present invention, thus generally described, will be understood more readily by reference to the following examples, which are provided by way of illustration and are not intended to be limiting of the present invention.

The examples below are provided to illustrate the subject invention. These examples are provided by way of illustration and are not included for the purpose of limiting the invention.

EXAMPLES

EXAMPLE I: cDNA Library Construction

cDNA library plates and clones originated from five cDNA libraries that were constructed by directional cloning. These are available through the Resource Center (http://www.rzpd.de) of the German Genome Project. In particular, the hfbr2 (human fetal brain; RZPD number DKFZp564) and hfkd2 (human fetal kidney; DKFZp566) libraries were generated using the Smart kit (Clontech), except that PCR was carried out with primers that contained uracil residues to permit directional cloning without restriction digestion and ligation, and were complementary with the pAMPl (LifeTechnologies) cloning sites for directional cloning. The htes3 (human testes; DKFZp434), hutel (human uterus; DKFZp586) and hmcfl (human mammary carcinoma; DKFZp727) libraries are conventional (Gubler, U., Hoffman, B.J., (1983), A simple and very efficient method for generating cDNA libraries. Gene 25, 263-269), size-selected cDNA libraries. They are cloned into pSPORTl (LifeTechnologies) via a Notl site which is introduced during reverse transcription downstream of the oligo dT primer and a Sail site that is introduced by the ligation of a adapters. The human mammary carcinoma library was constructed fgrom MCF7 cells.

The cDNA sequences of this application were first identified among the sequences comprising various libraries. Technology has advanced considerably since the first cDNA libraries were made. Many small variations in both chemicals and machinery have been instituted over time, and these have improved both the efficiency and safety of the process. Although the cDNAs could be obtained using an older procedure, the procedure presented in this application is exemplary of one currently being used by persons skilled in the art. For the purpose of providing an exemplary method, the mRNA isolation and cDNA library construction described here is for the MCF-7 library (DKFZp727) from which the clones named DKFZphmcfl xxyyxx were obtained.

The human cell line MCF-7 was grown in DMEM supplemented with 10% fetal calf serum until confluency. 3 X 10⁸ cells were harvested with a cell scraper in PBS. Cells were lysed in buffer containing 0.5 % NP-40 to leave the nuclei intact. The debris was pelleted by centrifugation at 15 000 x g for 10 minutes at 4 degrees Celsius. Proteins in the supernatant were degraded in presence of SDS and Proteinase K (30 minutes at 56 degrees Celsius). Precipitation of proteins was done in a Phenol/Chloroform extraction, RNA was precipitated from the aqueous phase with Na-acetate and Ethanol. Polyadenylated messages were isolated using Qiagen Oligotex (QIAGEN, Hilden Germany).

First strand cDNA synthesis was accomplished using an oligo (dT) primer which also contained an Notl restriction site. Second strand synthesis was performed using a combination of DNA polymerase I, E. coli ligase and RNase H, followed by the addition of a Sail adaptor to the blunt ended cDNA. The Sail adapted, double-stranded cDNA was then digested with Notl restriction enzyme, and fractionated by size on an agarose gel. DNA of the appropriate size was cut from the gel and cast into a second gel in a 90° angle. After electrophoresis in the second dimension, cDNA of the appropriate size was cut from the gel. The agarose block was broken down with help of gelase. The cDNA was purified with help of two phenol extractions and an ethanol precipitation. The cDNA was ligated into Sall/Notl pre-digested pSportl vector (LifeTechnologies) and transformed into DH10B bacteria.

The libraries were arrayed into 384-well microtiter plates and spotted on high density nylon membranes for hybridization analysis. Filters and clones are available through the Resource Center. Whole plates were distributed to the sequencing partners of the consortium for systematic sequencing.

EXAMPLE II: Sequencing of cDNA Clones

All clones in the 384-well microtiter plates were sequenced from the 5' end. Sequencing was done preferentially using dye terminator chemistry (ABD or Amersham) on ABI automated DNA sequencers (ABI 377, Applied Biosystems), one partner used EMBL prototype instruments (Arakis) mainly with dye primer chemistry.

The resulting expressed sequence tag (EST) sequences ("rl ESTs" = sequenced from 5 '-end) were analysed for:

a) the lack of identical matches with known genes.

For this, the EST-sequence was blasted against the cDNA consortiums own database and after that against public databases and (with BLASTn and BLASTx against EMBL/EMBLNEW and assembled ESTs, please refer to EXAMPLE III: Bioinformatics analysis of full length cDNAs, for description and parameter settings). ESTs which were identical to known genes in more than 100 bp, with less than 2 mismatches, were excluded from further analysis.

b) the presence of an open reading frame

Open reading frames (ORFs) were detected with an tool developed by Munich Information Center for Protein Sequences (MIPS) called ORF-map. ORF-map visualises potential start and stop-codons. If an ORF without a stop codon was detected in a rl-EST, the sequence was processed further.

c) the presence of GC rich sequences

A script developed by MIPS computed the GC -content of the rl -sequence, which should be >40%. Writing similar scripts is within the ordinary skill of one in bioinformatics.

d) the lack of repeat structures

Repeats such as Alu, Line or CA-repeats were detected by blasting (BLASTn and BLASTx, please refer to EXAMPLE III: Bioinformatics analysis of full length cDNAs, for description and parameter settings) against a repeat-database compiled by MIPS. If a repeat was present within the rl -sequence, the sequence were not processed further.

Novel clones that met all criteria were identified to the sequencers, who then performed 3 '-end sequencing of these clones. The resulting 3' ESTs ("si ESTs" = sequenced from 3 '-end) were checked for a) the lack of matches with known genes in public databases, and sequences already generated by us.

This was done by blasting against EMBL/EMBLNEW and assembled EST (BLASTn and BLASTx, please refer to EXAMPLE III: Bioinformatics analysis of full length cDNAs, for description and parameter settings).

b) the presence of polyadenylation signals.

Again only clones matching the selection criteria were chosen to be sequenced completely by the sequencers. Clones were selected after the following criteria:

A very good ORF had at least one BLASTx match to other proteins. A "good ORF" should extend to the 3' end and be longer than ~40 codons. If the ORF started in the rl sequence, in front of the potential start codon, there should not exist too many competing start codons in frame with the ORF start codon and the start should match the Kozak consensus ATG. If the EST sequence was to short to decide according to the potential ORF, and there were only a few or no start codons in the sequence the GC content of the Sequence should be greater than 40%. The rl sequences needed not contain an polyA-tail at the 3' end. In addition, the results of the blasting against the assembled human ESTs could help in questionable cases to decide whether to stop or to continue. A hit against these ESTs was an indication to go further.

Clones passing the above-described screening were sequenced in full. Sequencing was done preferentially using dye terminator chemistry (ABD or Amersham) on ABI automated DNA sequencers (ABI 377, Applied Biosystems), one partner used EMBL prototype instruments (Arakis) mainly with dye primer chemistry. Primer walking (Strauss et al., 1986, Specific-primer-directed DNA sequencing. Anal Biochem. 154, 353-360) was the preferred sequencing strategy because of the lower redundancy possible compared to random shotgun (Messing, J., Crea, R., Seeburg, H.P. (1981) A system for shotgun DNA sequencing. Nucleic Acids Res. 9, 32-39) methods. Walking primers were generally designed using software (e.g. Haas, S., Vingron, M., Poustka, A., Wiemann, S. (1998) Primer design in large-scale sequencing. Nucleic Acids Res. 26, 3006-3012, Schwager, C, Wiemann, S., Ansorge, W. (1995) GeneSkipper: integrated software environment for DNA sequence assembly and alignment. HUGO Genome Digest 2, 8-9) that permitted complete automation of this usually time consuming process and helped in the parallel processing of large numbers of clones.

EXAMPLE III: Bioinformatics analysis of full length cDNAs

Each sequence obtained was compared on nucleotide level in a stepwise manner to sequences in EMBL/EMBLNEW, EMBL-EST, EMBL-STS using the BLASTn algorithm. Basic Local Alignment Search Tool (BLAST, Altschul S. F. (1993) J Mol Evol 36:290-300; Altschul, S. F. et al (1990) J Mol Biol 215:403-10) is used to search for local sequence alignments. BLAST produces alignments of both nucleotide (BLASTn) and amino acid sequences (BLASTp or BLASTx) to determine sequence similarity. BLAST is especially useful in determining exact matches or in identifying homologs, because of the local nature of the alignments. While it is useful for matches which do not contain gaps, it is inappropriate for performing motif-style searching. The fundamental unit of BLAST algorithm output is the High-scoring Segment Pair (HSP).

An HSP consists of two sequence fragments of arbitrary but equal lengths whose alignment is locally maximal and for which the alignment BLAST approach is to look threshold or cut off score set by the user. BLAST looks for HSPs between a query sequence and a database sequence, to evaluate the statistical significance of any matches found, and to report only those matches which satisfy the user-selected threshold of significance. The parameter E establishes the statistically significant threshold for reporting database sequence matches. E is interpreted as the upper bound of the expected frequency of chance occurrence of an HSP (or set of HSPs) within the context of the entire database search. Any database sequence whose match satisfies E is reported in the program output. Parameter settings for the BLAST-operations (BLASTN 2.0al9MP-WashU) described were: EMBL-EMBLNEW: H=0 V=5 B=5 -filter seg; EMBL-EST: H=0 E=le-10 B=500 V=500 -filter seg; EMBL-STS: H=0 V=5 B=5.

Search against EMBL/EMBLNEW was done to determine whether the cDNAs are already known, and also to find out whether the cDNAs are encoded by genomic sequences already sequenced and published/submitted to these databases. Search against EMBL-EST was performed to get a first impression how abundant a particular cDNA would be and to get information on tissue specificity (so-called "electronic Northern-Blot", e.g. some of the cDNAs derived of the testis library show only hits to ESTs also derived of testis libraries).

The cDNA-sequences were blasted against EMBL-STS to determine STS-sequence- match to the cDNA, thus providing a mapping information to the new cDNA.

The potential protein-sequences were generated automatically by a script searching for the longest open reading frame (ORF) in each of the three forward frames with a minimum length of 90 codons. Next, the automatically generated ORFs were translated into protein sequences. These protein sequences were searched against the non redundant protein data set of PIR/SwissProt/Trembel/Tremblnew (BLASTP 2.0al9MP-WashU, parameter setting: V=7 B-7 H=0 -filter seg). If the script generated more than one ORF, one ORF was chosen manually by the annotater according to the degree of similarity to known proteins, the location of the ORF in the cDNA, the length, the amino acid composition and the content of Prosite-Motifs.

Additionally there was a BLASTx (BLASTX 2.0al9MP-WashU against non redundant protein database comprising PIR/SWISSPROT/TREMBL/TREMBLNEW; parameter-settings were: matrix/home/data/blast/matrix aa/BLOSUM62 H=0 V=5 B=5 -filter seg) search to find potential frame shift in the complementary eds of the cDNAs and to identify unspliced or partly spliced cDNAs. The protein sequence was then transferred to the PEDANT system, in order to generate additional information on the new proteins. PEDANT (Protein Extraction, Description, and ANalysis Tool, Frishman, D. & Mewes, H.-W. (1997) PEDANTic genome analysis. Trends in Genetics , 13, 415-416) is a platform developed at the Munich Information Center for Protein Sequences (MIPS, Munich, Germany), which incorporates practically all bioinformatics methods important for the functional and structural characterisation of protein sequences. Computational methods used by PEDANT are: FASTA

Very sensitive protein sequence database searches with estimates of statistical significance. Pearson W.R. (1990) Rapid and sensitive sequence comparison with FASTP and FASTA. Methods Enzymol. 183, 63-98.

BLAST2

Very sensitive protein sequence database searches with estimates of statistical significance. Altschul S.F., Gish W., Miller W., Myers E.W., and Lipman D.J. Basic local alignment search tool. Journal of Molecular Biology 215, 403-10.

PREDATOR

High-accuracy secondary structure prediction from single and multiple sequences. Frishman, D. and Argos, P. (1997) 75% accuracy in protein secondary structure prediction. Proteins, 27, 329-335. Frishman, D. and Argos, P.(1996) Incorporation of long-distance interactions in a secondary structure prediction algorithm. Prot. Eng. 9, 133-142.

STRIDE

Secondary structure assignment from atomic coordinates. Frishman, D. and Argos, P. (1995) Knowledge-based secondary structure assignment. Proteins 23, 566-579.

CLUSTALW

Multiple sequence alignment. Thompson, J.D., Higgins, D.G. and Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22:4673-4680.

TMAP

Transmembrane region prediction from multiply aligned sequences. Persson, B. and Argos, P. (1994) Prediction of transmembrane segments in proteins utilising multiple sequence alignments. J. Mol. Biol. 237, 182-192. ALOM2

Transmembrane region prediction from single sequences. Klein, P., Kanehisa, M., and DeLisi, C. Prediction of protein function from sequence properties: A discriminant analysis of a database. Biochim. Biophys. Acta 787, 221-226 (1984). Version 2 by Dr. K. Nakai.

SIGNALP

Signal peptide prediction Nielsen, H., Engelbrecht, J., Brunak, S., and von Heijne, G (1997). Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Engineering 10, 1-6.

SEG

Detection of low complexity regions in protein sequences. Wootton, J.C., Federhen, S. (1993) Statistics of local complexity in amino acid sequences and sequence databases. Computers & Chemistry 17, 149-163.

COILS

Detection of coiled coils. Lupas, A., M. Van Dyke, and J. Stock, "Predicting Coiled Coils from Protein Sequences." Science (1991) 252, 1162-1164.

PROSEARCH

Detection of PROSITE protein sequence patterns. Kolakowski L.F. Jr., Leunissen J.A.M., Smith J.E. (1992) ProSearch: fast searching of protein sequences with regular expression patterns related to protein structure and function. Biotechniques 13, 919-921.

BLIMPS

Similarity searches against a database of ungapped blocks. J.C. Wallace and Henikoff S., (1992) PATMAT: a searching and extraction program for sequence, pattern and block queries and databases, CABIOS 8, 249-254. Written by Bill Alford.

HMMER Hidden Markov model software . Sonnhammer E.L.L., Eddy S.R., Durbin R. (1997) Pfam: A Comprehensive Database of Protein Families Based on Seed Alignments. Proteins 28, 405-420.

pi

Perl script that returns the amino acid composition, molecular weight, theoretical pi, and expected extinction coefficient of an amino acid sequence. By Fred Lindberg. The parameter-settings were as follows: known3d: score > 100; BLAST: E-value < 10; SCOP: <= 50 Alignments, E-Value < 0.0001 ; signalp: Y=0.7; untersucht vom N-Terminus her: 50 aa; funcat: E-value < 0.001; BLOCKS: <= 10 hits; BLIMPS: threshold 1100.0; COILS: threshold 0.95; SEG: threshold 20.0; BLAST in report: E-value < 0.001; PIR-KW, superfamilies, EC- Nummern in report: E-value < 0.00001; known3d in report: score > 120

The results of PEDANT analysis, together with the results of the similarity searches, constitute the basis for the structural and functional annotation of the cDNAs and the encoded proteins, as specified below.

EXAMPLE III: CELLULAR LOCALIZATIONS OF GFP-FUSION PROTEINS

Plasmids of cDNA-GFP fusions were transfected into mammalian tissue culture cells and allowed to express the proteins for up to 48 hours. Live cells were imaged at 24 hours and 48 hours after transfection and the localisations recorded. The chart, below, depicts the apparent final cellular localisations of 107 cDNA-GFP fusions.

In order to minimize the possibility of the GFP interfering with protein function and/or localization, two separate populations of cDNAs were generated encoding N-terminal or C-terminal GFP fusions. Clearly this appears to be a crucial strategy, since overall only 56% of the proteins localised to a specific compartment irrespective of the position of the GFP. In the instances where only one fusion localized, the complementary fusion either gave no expression or a nuclear and cytosolic staining - characteristic for GFP alone expression.

Each cDNA in turn was subjected to bioinformatic analysis. Where possible, the potential subcellular localisations of the expressed proteins were determined. This information was then compared to the actual localisations determined from expression of the GFP-fusion proteins in mammalian cells.

DKFZphfbr₂_16cl6

group: Cell structure and motility

DKFZphfbr₂_16cl6 ,₃ encodes a novel 586 amino acid¹ protein with .similarity to the human actin binding protein MAYVEN and Drosophila Kelch. AVEN is a novel actin binding protein predominantly expressed in brain. Drosophila kelch is involved in the maintenance of ring canal organization during oogenesis. The amino half of the protein including the BTB domain mediates dimerization, while the amino half might allow cross-linking of ring canal actin filaments, thus organising the inner rim cytoskeleton. The kelch repeat domain is necessary for ring canal localisation and believed to mediate an additional interaction, possibly with actin. The new protein shares the features of both proteins and therefore should be involved in the organisation of cyto skeleton binding to membrane proteins .

The new protein can find application in modulating/blocking of cyto skeleton-membrane protein interaction.

similarity to Drosophila kelch complete cDNA, complete eds, EST hits on genomic level partly encoded by AC00508 and AC006039

Sequenced by Qiagen

Locus : unknown

Insert length: ₃028 bp

Poly A stretch at pos . ₃004, polyadenylation signal at pos . 2984

1 GGGGGCCCGG GGACGCAGCC CAGTTGGTAG CGTCGCTCCC TGAGCGTTTC

51 TAAGGGGGCC GCCCGGCCCT GTCTTTCGGC AGTGGCCGAG CCACCGCCGC

101 CTGCCGCGCG TTCCAGAGCT GGGCGCTGCA GCTGCACTGC CGATCGCCGT

151 GTTTGGTCGA TAGAATCCCC AGTGTGCCCA GAGAGTGCGA CCCCTCGCCC

201 GGCCCGGCGA GCCCCGGGCG TGAACCGAGC TGAGGGAGGA TGGCAGCCTC

251 TGGGGTGGAG AAGAGCAGCA AGAAGAAGAC CGAGAAGAAA CTTGCTGCTC

301 GGGAAGAAGC TAAATTGTTG GCGGGTTTCA TGGGCGTCAT GAATAACATG

351 CGGAAACAGA AAACGTTGTG TGACGTGATC CTCATGGTCC AGGAAAGAAA

401 GATACCTGCT CATCGTGTTG TTCTTGCTGC AGCCAGTCAT TTTTTTAACT 451 TAATGTTCAC AACTAACATG CTTGAATCAA AGTCCTTTGA AGTAGAACTC 501 AAAGATGCTG AACCTGATAT TATTGAACAA CTGGTGGAAT TTGCTTATAC 551 TGCTAGAATT TCCGTGAATA GCAACAATGT TCAGTCTTTG TTGGATGCAG 601 CAAACCAATA TCAGATTGAA CCTGTGAAGA AAATGTGTGT TGATTTTTTG 651 AAAGAACAAG TTGATGCTTC AAATTGTCTT GGTATAAGTG TGCTAGCGGA 701 GTGTCTAGAT TGTCCTGAAT TGAAAGCAAC TGCAGATGAC TTTATTCATC 751 AGCACTTTAC TGAAGTTTAC AAAACTGATG AATTTCTTCA ACTTGATGTC 801 AAGCGAGTAA CACATCTTCT CAACCAGGAC ACTCTGACTG TGAGAGCAGA 851 GGATCAGGTT TATGATGCTG CAGTCAGGTG GTTGAAATAC ^' GATGAGCCTA 901 ATCGCCAGCC ATTTATGGTT GATATCCTTG CTAAAGTCAG GTTTCCTCTT 951 ATATCAAAGA ATTTCTTAAG TAAAACGGTA CAAGCTGAAC CACTTATTCA

1001 AGACAATCCT GAATGCCTTA AGATGGTGAT AAGTGGAATG AGGTACCATC 1051 TACTGTCTCC AGAGGACCGA GAAGAACTTG TAGATGGCAC AAGACCTAGA 1101 AGAAAGAAAC ATGACTACCG CATAGCCCTA TTTGGAGGCT CTCAACCACA 1151 GTCTTGTAGA TATTTTAACC CAAAGGATTA TAGCTGGACA GACATCCGCT 1201 GCCCCTTTGA AAAACGAAGA GATGCAGCAT GCGTGTTTTG GGACAATGTA 1251 GTATACATTT TGGGAGGCTC TCAGCTTTTC CCAATAAAGC GAATGGACTG 1301 CTATAATGTA GTGAAGGATA GCTGGTATTC GAAACTGGGT CCTCCGACAC 1351 CTCGAGACAG CCTTGCTGCA TGTGCTGCAG AAGGCAAAAT TTATACATCT 1401 GGAGGTTCAG AAGTAGGAAA CTCAGCTCTG TATTTATTTG AGTGCTATGA 1451 TACGAGAACT GAAAGCTGGC ACACAAAGCC CAGCATGCTG ACCCAGCGCT 1501 GCAGCCATGG GATGGTGGAA GCCAATGGCC TAATCTATGT TTGTGGTGGA 1551 AGTTTAGGAA ACAATGTTTC AGGGAGAGTG CTTAATTCCT GTGAAGTTTA 1601 TGATCCTGCC ACAGAAACAT GGACTGAGCT GTGTCCAATG ATTGAAGCCA 1651 GGAAGAATCA TGGGCTGGTA TTTGTAAAAG ACAAGATATT TGCTGTGGGT 1701 GGTCAGAATG GTTTAGGTGG TCTGGACAAT GTGGAATATT ACGATATTAA 1751 GTTGAACGAA TGGAAGATGG TCTCACCAAT GCCATGGAAG GGTGTAACAG 1801 TGAAATGTGC AGCAGTTGGC TCTATAGTTT ATGTCTTGGC TGGTTTTCAG 2₃01 AGAAGATTGG CTCATCAGTG AAGCGCAGTA TCTTAGCTCT AGATTCTATT

₂₃51 TTCATGCATC ACAGAAGTGC TATACGGTTA GGTCTGTTTG TGCTCAGTCA

₂401 AGAACTAAGA AATAGTATGA ATTGTAAGTC AAGATGGGCA ACTCAGATGG

₂451 AGCAGCTTAG TCTCACAGTT TGCTTGTCTA TTTATTTTAT TTAGTGCCAA

2501 ATGTATTCCA TTTTAAAAGT AAGCCAGAGT GAGTCAAGGC ATATACACAC

₂551 TTTCTCACAA AACTTCCTAA ACAGATTTGG GGGTTTAATA TGTCCAACTC

₂601 CTCATGAAAT ATATTCAATC CACTTAAATA TATTCCATCT TTTTAACATA

₂651 AAATGTAAAG CTTAGCACCC ATCATTAATT TATGTCTCTG TTTTATCCAG

2701 TGGTTAAAAA AGGATTCTGC CTCTTTAGTC CTCACTGTTA AATAAAACCC

2751 AATCATAGTA AGTGATTAAC TAGCAAAAAG TAAAGCTATT TATAGCAAAT

2801 TTCTAGATCA TTAGAAAAGC ACTGGTAGTT GTACAATATC AGTGTTGACT

2851 TTGAACTTCT TTAACGAGAT CATGAATTCT TTTCCCTTAG CCAAAACATG

2901 AAATATTTAA CCTAGTTGTC TCTAAAAGTT TTGTAATCAT GAGTTAGATA

2951 TATGTCATCT CCTATTCATT GCTTTTATGT GATCAATAAA TCTTTTACAA

3001 ACCCAAAAGA AAAAAAAAAA AAAAAAAA

BLAST Results

Entry AC005082 from database EMBL:

Homo sapiens clone RG271G13; HTGS phase 1, 7 unordered pieces.

Score = 6460, P = O.Oe+00, identities = 1292/1292

4 exons matching Bp 1180-3007

Entry AC006039 from database EMBL:

*** SEQUENCING IN PROGRESS *** Homo sapiens clone NH0319F03; HTGS phase

1, 3 unordered pieces.

Score = 1780, P = 2.0e-117, identities = 368/377

5 exons matching Bp 6-860

Entry HSG20603 from database EMBL: human STS A005Y34. Score = 670, P = 1.0e-23, identities = 134/134

Medline entries

93201592: kelch encodes a component of intercellular bridges in

Drosophila egg chambers .

97412177:

Drosophila kelch is an oligomeric ring canal actin organizer.

Peptide information for frame 3

ORF from 240 bp to 1997 bp; peptide length: 586 Category: strong similarity to known protein

1 MAASGVEKSS KKKTEKKLAA REEAKLLAGF MGVMNNMRKQ KTLCDVILMV

51 QERKIPAHRV VLAAASHFFN LMFTTNMLES KSFEVELKDA EPDIIEQLVE

101 FAYTARISVN SNNVQSLLDA ANQYQIEPVK KMCVDFLKEQ VDASNCLGIS

151 VLAECLDCPE LKATADDFIH QHFTEVYKTD EFLQLDVKRV THLLNQDTLT

201 VRAEDQVYDA AVRWLKYDEP NRQPFMVDIL AKVRFPLISK NFLSKTVQAE

251 PLIQDNPECL KMVISGMRYH LLSPEDREEL VDGTRPRRKK HDYRIALFGG

301 SQPQSCRYFN PKDYS TDIR CPFEKRRDAA CVFWDNVVYI LGGSQLFPIK

351 RMDCYNVVKD S YSKLGPPT PRDSLAACAA EGKIYTSGGS EVGNSALYLF

401 ECYDTRTES HTKPSMLTQR CSHGMVEANG LIYVCGGSLG NNVSGRVLNS

451 CEVYDPATET WTELCPMIEA RKNHGLVFVK DKIFAVGGQN GLGGLDNVEY

501 YDIKLNE KM VSPMPWKGVT VKCAAVGSIV YVLAGFQGVG RLGHILEYNT

551 ETDKWVANSK VRAFPVTSCL ICVVDTCGAN EETLET

BLASTP hits

Entry KELC_DROME from database SWISSPROT:

RING CANAL PROTEIN (KELCH PROTEIN) .

Length = 689

Score = 816 (287.2 bits), Expect = 1.9e-81, P = 1.9e-81

Identities = 187/542 (34%), Positives = 290/542 (53%)

Entry AC004021_1 from database TREMBL:

WUGSC:H_DJ0186K10.1"; Human PAC clone DJ0186K10 from 5q31, complete sequence. Homo sapiens (human) Length = 497 Score = 704 (247.8 b ts). Expect = 1.4e-69, P = 1.4e-69 Identities = 163/483 (33%), Positives = 253/483 (52%)

Entry HSDKG12_1 from database TREMBL:

"KIAA0132"; Human mRNA for KIAA0132 gene, complete eds. Homo sapiens (human)

Length = 624

Score = 692 (243.6 bits), Expect = 2.6e-68, P = 2.6e-68

Identities = 175/527 (33%), Positives = 272/527 (51%)

Entry A45773 from database PIR: kelch protein, long form - fruit fly (Drosophila melanogaster)

Length = 1476

Score = 817 (287.6 bits), Expect = 1.7e-80, P = 1.7e-80

Identities = 189/549 (34%), Positives = 292/549 (53%)

Alert BLASTP hits for DKFZphfbr2_16cl6, frame 3 No Alert BLASTP hits found Pedant information for DKFZphfbr2_16cl6, frame 3

Report for DKFZphfbr2_16cl6.3

[LENGTH] 586 [MW] 65992.06 [pi] 6.08 [HOMOL] PIR:A45773 kelch protein, long form - fruit fly (Drosophila melanogaster) 5e-85

[BLOCKS] BL00075D Dihydrofolate reductase proteins

[SCOP] dlgog_3 2.46.1.1.1 (151-537) Galactose oxidase, central domai 6e-36

[PIRK ] zinc finger 2e-ll

[PIRK ] DNA binding 9e-10

[PIRKW] transcription factor le-06

[SUPFAM] A55R protein middle region homology le-35

[SUPFAM] POZ domain homology le-35

[SUPFAM] vaccinia virus 59K Hindlll-C protein 5e-15

[SUPFAM] A55R protein le-35

[SUPFAM] myxoma virus M9-R protein 2e-ll

[SUPFAM] A55R protein carboxyl-terminal homology le-35

[PROSITE] CAMP_PHOSPHO_SITE 2

[PROSITE] MYRISTYL 8

[PROSITE] CK2_PHOSPHO_SITE 10

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 11

[PROSITE] ASN_GLYCOSYLATION 1

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 3.75 %

SEQ MAASGVEKSSKKKTEKKLAAREEAKLLAGFMGVMNNMRKQKTLCDVILMVQERKIPAHRV SEG xxxxxxxxxxxxxxxxxxxxxx PRD ccceeeeccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhheeeeccccchhhhhe

SEQ VLAAASHFFNLMFTTNMLESKSFEVELKDAEPDIIEQLVEFAYTARISVNSNNVQSLLDA SEG PRD eeccccccccccccccchhhhhheeeeccccchhhhhhhhhhhhheeeeccchhhhhhhh

SEQ ANQYQIEPVKKMCVDFLKEQVDASNCLGISVLAECLDCPELKATADDFIHQHFTEVYKTD SEG PRD hhhhhhhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ EFLQLDVKRVTHLLNQDTLTVRAEDQVYDAAVRWLKYDEPNRQPFMVDILAKVRFPLISK SEG PRD hhhchhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhccch

SEQ NFLSKTVQAEPLIQDNPECLKMVISGMRYHLLSPEDREELVDGTRPRRKKHDYRIALFGG SEG PRD hhhhhhhhhhhccccccchhhhhhhhhhhhccccccccccccccccccccceeeeeeecc

SEQ SQPQSCRYFNPKDYSWTDIRCPFEKRRDAACVFWDNVVYILGGSQLFPIKRMDCYNVVKD SEG PRD ccccceeeccccccccccccccccccceeeeeeeceeeeeeccccccccceeeecccccc

SEQ SWYSKLGPPTPRDSLAACAAEGKIYTSGGSEVGNSALYLFECYDTRTESWHTKPSMLTQR SEG PRD cccccccccccccceeeeeccceeeeeccccccccceeeeeecccccccccccccccccc SEQ CSHGMVEANGLIYVCGGSLGNNVSGRVLNSCEVYDPATETWTELCPMIEARKNHGLVFVK SEG PRD ccceeeecceeeeeecccccccccccccceeeeccccccccccccccccccccceeeeec

SEQ DKIFAVGGQNGLGGLDNVEYYDIKLNEWKMVSPMPWKGVTVKCAAVGSIVYVLAGFQGVG SEG PRD ceeeecccccccccccceeeccccccceeecccccccccceeeeeccceeeeeccccccc

SEQ RLGHILEYNTETDKWVANSKVRAFPVTSCLICVVDTCGANEETLET SEG PRD cccceeecccccccccccccccccccceeeeeeeeccccccccccc

Prosite for DKFZphfbr2_16cl6.3

PS00001 442->446 ASN_GLYCOΞYLATION PDOC00001 PS00004 11->15 CAMP_PHOSPHO_SITE PDOC00004 PS00004 188->192 CAMP_PHOSPHO_SITE PDOC00004 PS00005 9->12 PKC_PHOSPHO_SITE PDOC00005 PS00005 10->13 PKC_PHOSPHO_SITE PDOC00005 PS00005 14->17 PKC_PHOSPHO_SITE PDOC00005 PS00005 104->107 PKC_PHOSPHO_SITE PDOC00005 PS00005 200->203 PKC_PHOSPHO_SITE PDOC00005 PS00005 305->308 PKC_PHOSPHO_SITE PDOC00005 PS00005 370->373 PKC_PHOSPHO_SITE PDOC00005 PS00005 418->421 PKC_PHOSPHO_SITE PDOC00005 PS00005 444->447 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 520->523 PKC_PHOSPHO_SITE PDOC00005 PS00005 ,552->555 PKC_PHOSPHO_SITE PDOC00005 PS00006 4->8 CK2_PHOSPHO_SITE PDOC00006 PS00006 42->46 CK2_PHOSPHO_SITE PDOC00006 PS00006 116->120 CK2_PHOSPHO_SITE PDOC00006 PS00006 164->168 CK2_PHOSPHO_SITE PDOC00006 PS00006 273->277 CK2_PHOSPHO_SITE PDOC00006 PS00006 315->319 CK2_PHOSPHO_SITE PDOC00006 PS00006 370->374 CK2_PHOSPHO_SITE PDOC00006 PS00006 405->409 CK2_PHOSPHO_SITE PDOC00006 PS00006 460->464 CK2_PHOSPHO_SITE PDOC00006 PS00006 550->554 CK2_PHOSPHO_SITE PDOC00006 PS00007 202->209 TYR_PHOSPHO_SITE PDOC00007 PΞ00008 5->ll MYRISTYL PDOC00008 PΞ00008 32->38 MYRISTYL PDOC00008 PS00008 389->395 MYRISTYL PDOC00008 PS00008 424->430 MYRISTYL PDOC00008 PS00008 436->442 MYRISTYL PDOC00008 PS00008 440->446 MYRISTYL PDOC00008 PS00008 487->493 MYRISTYL PDOC00008 PS00008 493->499 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_16cl6.3)

DKFZphfbr2_16f21

group: brain derived

DKFZphfbr2_16f21 encodes a novel 208 ammo acid protein with strong similarity to human zinc finger protein 216.

The novel protein shows strong similarity to the human zinc finger protein 216, but has no Zn finger.

PROSITE: Contains no Zinc finger; No informative BLAST results; no predictive prosite, pfam or SCOP motife

The new protein can find application in studying the expression profile of bram-specific genes . strong similarity to zinc finger protein 216 complete cDNA, complete eds, EST hits start matches Kozak consensus ANNatgG,

Sequenced by Qiagen

Locus : unknown

Insert length: 1512 bp

Poly A stretch at pos. 1490, polyadenylation signal at pos. 1474

1 GGGAGCAAGC AGGGGTTCGG CGGCATTACC TGTACCCATT CACCGGCGGC 51 TACCGGCGGC GGCGCGTAGC GTGTCAGGCG GAGAGACCCG CCGCCAGGTG

101 TGCAACTGAG GAACATGGCT CAAGAAACTA ATCACAGCCA AGTGCCTATG

151 CTTTGTTCCA CTGGCTGTGG ATTTTATGGA AACCCTCGTA CAAATGGCAT

201 GTGTTCAGTA TGCTATAAAG AACATCTTCA AAGACAGAAT AGTAGTAATG

251 GTAGAATAAG CCCACCTGCA ACCTCTGTCA GTAGTCTGTC TGAATCTTTA

301 CCAGTTCAAT GCACAGATGG CAGTGTGCCA GAAGCCCAGT CAGCATTAGA

351 CTCTACATCT TCATCTATGC AGCCCAGCCC TGTATCAAAT CAGTCACTTT

401 TATCAGAATC TGTAGCATCT TCTCAATTGG ACAGTACATC TGTGGACAAA

451 GCAGTACCTG AAACAGAAGA TGTGCAGGCT TCAGTATCAG ACACAGCACA

501 GCAGCCATCT GAAGAGCAAA GCAAGCCTCT TGAAAAACCG AAACAAAAAA

551 AGAATCGCTG TTTCATGTGC AGGAAGAAAG TGGGACTTAC TGGGTTTGAA

601 TGCCGGTGTG GAAATGTTTA CTGTGGTGTA CACCGTTACT CAGATGTACT

651 CAATTGCTCT TACAATTACA AAGCCGATGC TGCTGAGAAA ATCAGAAAAG

701 AAAATCCAGT AGTTGTTGGT GAAAAGATCC AAAAGATTTG AACTCCTGCT

751 GGAATACAAA ATTCTTGAGC ATCTGCAAAC TAAAAATTGA CTTGAGGTTT

801 TTTTTTTCCT AGTCATTGGG AATGTAGAGC AGTGTATCTT GCATGTCATC

851 GGAAGAATAG ATTTTTGTTT TGGTTTTGTT TTGAAAATGA CTCTGAACAT

901 TTATTTCCAT TGCAATTTCT GTGGCTGAGG AGACTTAAAC TTTACAAGTA

951 TTATCCTTTT AAGATCATTT TAATTTTAGT TGAGTGCAGA GGGCTTTTAT 1001 AACAAACGTG CAGAAATTTT GGAGGGCTGT GATTTTTCCA GTATTAAACA 1051 TGCATGCATT AATCTTGCAG TTTATTTTCT CATTATGTAT GTATATATCG 1101 CTTTTCTCTG CAGCACGATT TCTCTTTTGA TAATGCCCTT TAGGGCACAA 1151 CTAGTTATCA GTAACTGAAT GTATCTTAAT CATTATGGCT GCTTCTGTTT 1201 TTTCATTAAC AAAGGTTATT CATATGTTAG CATATAGTTT CTTTGCACCC 1251 ACTATTTATG TCTGAATCAT TTGTCACAAG AGAGTGTGTG CTGATGAGAT 1301 TGTAAGTTTG TGTGTTTAAA CTTTTTTTTG AGCGAGGGAA GAAAAAGCTG 1351 TATGCATTTC ATTGCTGTCT ACAGGTTTCT TTCAGATTAT GTTCATGGGT 1401 TTGTGTGTAT ACAATATGAA GAATGATCTG AAGTAATTGT GCTGTATTTA 1451 TGTTTATTCA CCAGTCTTTG ATTAAATAAA AAGGAAAACC AGAAAAAAAA 1501 AAAAAAAAAA AA

BLAST Results

No BLAST result

Medlme entries o Medlme entry

Peptide information for frame 1 ORF from 115 bp to 738 bp; peptide length: 208 Category: strong similarity to known protein

1 MAQETNHSQV PMLCSTGCGF YGNPRTNGMC SVCYKEHLQR QNSSNGRISP

51 PATSVSSLSE SLPVQCTDGS VPEAQSALDS TSSSMQPSPV SNQSLLSESV

101 ASSQLDSTSV DKAVPETEDV QASVSDTAQQ PSEEQSKPLE KPKQKKNRCF

151 MCRKKVGLTG FECRCGNVYC GVHRYSDVLN CSYNYKADAA EKIRKENPVV

201 VGEKIQKI

BLASTP hits

Entry ATF7H19_1 from database TREMBLNE : gene: "F7H19.10"; product: "putative protein"; Arabidopsis thaliana DNA chromosome 4, BAC clone F7H19 (ESSAII project) >TREMBL: ATT12H17_21 gene: "T12H17.210"; product: "predicted protein"; Arabidopsis thaliana

DNA chromosome 4, BAC clone T12H17 (ESSAII project)

Score = 206, P = 2.1e-24, identities = 51/146, positives = 77/146

Entry PVPVPR3A_1 from database TREMBL: gene: "PVPR3"; P.vulgaris PVPR3 protein mRNA, complete eds.

Score = 237, P = 4.9e-20, identities = 50/136, positives = 73/136

Entry AF062072_1 from database TREMBL: gene: "ZNF216"; product: "zinc finger protein 216"; Homo sapiens zinc finger protein 216 (ZNF216) gene, complete eds.

Score = 591, P = 1.6e-57, identities = 124/215, positives = 147/215

Alert BLASTP hits for DKFZphfbr2_16f21, frame 1

TREMBL:AF062071_1 product: "zinc finger protein ZNF216"; Mus musculus zinc finger protein ZNF216 mRNA, complete eds., N = 1, Score = 590, P = 2.1e-57

TREMBLNEW:AB001773_1 gene: "pem-6"; product: "PEM-6"; Ciona savignyi pem-6 (posterior end mark 6) mRNA, complete eds., N = 1, Score = 421, P = 1.7e-39

>TREMBL:AF062071_1 product: "zinc finger protein ZNF216"; Mus musculus zinc finger protein ZNF216 mRNA, complete eds. Length = 213

HSPs:

Score = 590 (88.5 bits), Expect = 2.1e-57, P = 2.1e-57 Identities = 123/213 (57%), Positives = 146/213 (68%)

Query: 1 MAQETNHSQVPMLCSTGCGFYGNPRTNGMCSVCYKEHLQRQNSSNGRISPPAT SVSS 57

MAQETN + PMLCSTGCGFYGNPRTNGMCSVCYKEHLQRQ +S GR+SP T S S Sb.ct: 1 MAQETNQTPGPMLCSTGCGFYGNPRTNGMCSVCYKEHLQRQQNS-GRMSPMGTASGSNSP 59

Query: 58 LSESLPVQCTDGSVPEAQSALDSTSSSMQPSPVSNQSLLSE—SVASSQLDSTSVDKAVP 115

S+S VQ D + + A STS + PV+ + + ++ S+ D + K Sb]Ct: 60 TSDSASVQRADAGLNNCEGAAGSTSEKSRNVPVAALPVTQQMTEMSISREDKITTPKT-E 118

Query: 116 ETEDVQASVSDTAQQPSEEQS—KPLEKPKQKKNRCFMCRKKVGLTGFECRCGNVYCGVH 173

+E V S + QPS QS K E PK KKNRCFMCRKKVGLTGF+CRCGN++CG+H Sb.ct: 119 VSEPVVTQPSPSVSQPSSSQSEEKAPELPKPKKNRCFMCRKKVGLTGFDCRCGNLFCGLH 178

Query: 174 RYSDVLNCSYNYKADAAEKIRKENPVVVGEKIQKI 208

RYSD NC Y+YKA+AA KIRKENPVVV EKIQ+I Sb₃ct: 179 RYSDKHNCPYDYKAEAAAKIRKENPVVVAEKIQRI 213

Pedant information for DKFZphfbr2_16 21, frame 1

Report for DKFZphfbr2_16 21.1

[LENGTH] 208

[MW] 22541.23

[pi] 6.80

[HOMOL] TREMBL :AF062072_1 gene: "ZNF216"; product: "zinc finger protein 216"; Homo sapiens zinc finger protein 216 (ZNF216) gene, complete eds. 9e-57

[PIRKW] zinc 8e-13

[PIRKW] zinc finger 8e-13 [PIRKW] fusion protein 8e-13

[SUPFAM] unassigned ubiquitin-related proteins 8e-13

[SUPFAM] ubiquitin homology 8e-13

[PROSITE] MYRISTYL 2

[PROSITE] CK2_PHOSPHO_SITE 7

[PROSITE] ASN_GLYCOSYLATION 4

[KW] Irregular

[KW] LOW_COMPLEXITY 7.21 %

SEQ MAQETNHSQVPMLCSTGCGFYGNPRTNGMCSVCYKEHLQRQNSSNGRISPPATSVSSLSE

SEG PRD ccccccccccccccccccccccccccccccchhhhhhhhhhccccccccccccccccccc

SEQ SLPVQCTDGSVPEAQSALDSTSSSMQPSPVSNQSLLSESVASSQLDSTSVDKAVPETEDV SEG xxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ QASVSDTAQQPSEEQSKPLEKPKQKKNRCFMCRKKVGLTGFECRCGNVYCGVHRYSDVLN SEG PRD cccccccccccccccccccccccccccceeecccccccceeecccccccccccccccccc

SEQ CSYNYKADAAEKIRKENPVVVGEKIQKI SEG PRD ccchhhhhhhhhhhhhcccccccccccc

Prosite for DKFZphfbr2_16f21.1

PS00001 6->10 ASN_GLYCOSYLATION PDOC00001

PS00001 42->46 ASN_GLYCOSYLATION PDOC00001

PS00001 92->96 ASN_GLYCOSYLATION PDOC00001

PS00001 180->184 ASN_GLYCOSYLATION PDOC00001

PS00006 57->61 CK2_PHOSPHO_SITE PDOC00006

PS00006 70->74 CK2_PHOSPHO_SITE PDOC00006

PS00006 76->80 CK2_PHOSPHO_SITE PDOC00006

PS00006 103->107 CK2_PHOSPHO_SITE PDOC00006

PS00006 108->112 CK2_PHOSPHO_SITE PDOC00006

PS00006 123->127 CK2_PHOSPHO_SITE PDOC00006

PS00006 159->163 CK2_PHOSPHO_SITE PDOC00006

PS00008 22->28 MYRISTYL PDOC00008

PS00008 166->172 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_16f21.1)

DKFZphfbr2_16gl8

group: cell cycle

DKFZphfbr2_16gl8.3 encodes a novel 984 amino acid protein with similarity to centromeric proteins of yeasts.

The novel protein shows similarity to S. pombe SPAC17A5.07c and the S. cerevisiae Smt4p suppressor of MIF2 gene. MIF2 encodes a centromeric protein with homology to the mammalian centromeric protein CENP-C. Mutations in MIF2 stabilise dicentric rainichromosomes and confer high instability to chromosomes that bear a cis-acting mutation in element I of the yeast centromeric DNA (CDEI). Therefore the new protein should be involved in centromer organisation, too.

The new protein can find application in modulating/blocking the cell cycle and influencing the behavior of chromosomes, both natural and artificial in eukaryotic cells. similarity to KIAA0797 and yeast Smt4p complete cDNA, complete eds, EST hits the yeast Smt4 protein seems to be involved in centromer function and microtuble organisation

Sequenced by Qiagen

Locus: unknown

Insert length: 4826 bp

Poly A stretch at pos. 4756, polyadenylation signal at pos. 4736

1 GGGTCGAGGT CGACGGTATC GATAAGTTTT TTTTTTTTTT TTTTTTTTTT 51 TTTTCCTTTC CCCTCCCCCT CCCTCTCCAA GCCGGAGGGG TCCTGAGGTG

101 ACAGCGCCTG CAACTGAAAT TTCAGCAGCG GGAGAAGATG GACAAGAGAA

151 AGCTCGGGCG ACGGCCATCT TCATCCGAAA TCATCACAGA AGGAAAAAGG

201 AAAAAGTCAT CTTCTGATTT ATCGGAGATA AGAAAGATGT TAAATGCAAA

251 ACCAGAGGAT GTCCATGTTC AATCACCACT GTCCAAATTC AGAAGCTCAG

301 AACGCTGGAC TCTCCCTTTG CAGTGGGAAA GAAGCCTAAG GAATAAAGTC

351 ATCTCTCTAG ACCATAAAAA TAAAAAACAT ATCCGAGGGT GTCCTGTTAC

401 TTCCAGGTCA TCACCAGAAA GGATACCCAG AGTTATATTG ACGAATGTCC

451 TGGGAACGGA GTTAGGAAGA AAATACATAA GGACCCCACC TGTAACTGAG

501 GGAAGTTTGA GTGATACAGA CAACTTGCAA TCAGAGCAAC TTTCTTCATC

551 ATCTGATGGC AGCCTAGAAT CTTATCAAAA TCTAAACCCT CACAAGAGCT

601 GTTATTTATC TGAAAGGGGC TCACAACGAA GTAAGACAGT AGATGACAAT

651 TCTGCAAAGC AGACTGCGCA CAATAAAGAA AAACGAAGAA AGGATGATGG

701 CATTTCTCTT TTAATATCTG ATACTCAGCC TGAAGACCTT AACAGTGGAA

751 GTAGAGGTTG TGATCATCTC GAACAGGAAA GCAGAAACAA GGATGTTAAA

801 TATTCTGATT CAAAAGTGGA ACTCACTCTG ATTTCCAGGA AGACAAAGAG

851 AAGGCTTAGA AATAATTTAC CTGATTCTCA ATATTGTACT TCTTTGGATA

901 AGTCAACAGA ACAGACAAAA AAACAAGAAG ATGACTCAAC AATATCCACT

951 GAGTTTGAAA GGCCAAGTGA AAACTATCAT CAGGATCCAA AACTGCCTGA 1001 AGAAATTACA ACTAAACCTA CAAAAAGTGA TTTTACTAAG CTATCCTCAC 1051 TTAACAGTCA GGAGTTGACT TTGAGTAATG CCACCAAAAG TGCCTCTGCC 1101 GGTTCAACCA CTGAAACCGT TGAGTACTCT AATTCCATTG ATATTGTGGG 1151 GATTTCTTCC CTGGTTGAGA AGGATGAGAA TGAGTTGAAT ACCATAGAAA 1201 AGCCTATTCT AAGAGGACAT AATGAAGGGA ACCAATCACT GATCTCAGCT 1251 GAACCAATTG TTGTTTCCAG TGATGAAGAA GGACCTGTTG AACATAAAAG 1301 TTCAGAAATT CTTAAGTTAC AATCTAAGCA AGACCGTGAG ACAACTAATG 1351 AAAATGAGAG TACTTCTGAA TCAGCATTGT TAGAACTACC ATTGATTACA 1401 TGTGAATCTG TACAGATGTC ATCTGAATTA TGCCCATATA ATCCTGTCAT 1451 GGAGAACATT TCCAGTATTA TGCCTAGTAA TGAGATGGAT CTACAACTGG 1501 ATTTTATATT TACTTCTGTT TATATTGGTA AAATAAAAGG AGCTTCTAAA 1551 GGTTGTGTTA CAATCACAAA AAAATATATT AAGATCCCAT TTCAAGTGTC 1601 CCTGAATGAG ATTTCATTGC TAGTGGATAC CACACATTTA AAGCGGTTTG 1651 GGTTATGGAA AAGTAAGGAT GATAATCACA GTAAAAGGAG TCATGCTATT 1701 CTTTTCTTCT GGGTCTCTTC AGATTATCTT CAAGAGATTC AGACCCAATT 1751 AGAACACTCT GTATTAAGCC AGCAATCAAA ATCTAGTGAA TTCATTTTCC 1801 TTGAACTACA CAATCCTGTT TCACAGAGAG AAGAATTGAA GCTGAAAGAT 1851 ATTATGACGG AAATAAGTAT AATCAGTGGA GAATTAGAGC TTTCTTACCC 1901 GTTGTCTTGG GTTCAGGCAT TTCCTTTGTT TCAGAACCTC TCTTCAAAAG 1951 AAAGTTCTTT TATTCATTAT TACTGTGTTT CAACTTGTTC TTTCCCTGCT 2001 GGTGTTGCTG TTGCTGAAGA AATGAAGCTG AAATCAGTAT CTCAGCCCTC 2051 AAACACAGAT GCGGCCAAGC CTACTTACAC CTTCCTGCAG AAGCAAAGTA 2101 GCGGTTGCTA CTCCCTTTCT ATTACATCTA ATCCAGATGA AGAATGGCGG 2151 GAAGTCAGGC ACACTGGACT TGTTCAGAAG TTGATTGTAT ATCCTCCACC 2201 ACCTACTAAG GGGGGATTGG GAGTAACTAA TGAAGATCTG GAGTGTTTAG 2251 AAGAAGGAGA GTTTCTTAAT GATGTAATCA TTGATTTTTA CCTTAAGTAT 2301 CTTATATTGG AGAAGGCATC AGATGAACTT GTTGAACGAA GTCACATTTT 2351 TAGTAGCTTT TTCTATAAAT GCTTGACAAG AAAGGAAAAT AATTTAACAG

2401 AAGATAATCC AAATCTTTCA ATGGCACAGA GAAGACATAA AAGAGTAAGA

2451 ACATGGACTC GTCACATAAA CATTTTTAAT AAAGATTACA TCTTTGTACC

2501 TGTAAATGAG TCGTCTCACT GGTATCTCGC AGTCATTTGT TTTCCATGGT

2551 TAGAAGAAGC TGTGTATGAA GATTTTCCAC AAACTGTATC CCAGCAGTCC

2601 CAGGCTCAGC AGTCCCAAAG TGACAACAAA ACAATAGATA ATGATCTACG

2651 TACTACTTCG ACACTGTCTT TGAGTGCAGA GGATTCCCAA AGTACCGAGT

2701 CGAATATGTC AGTACCAAAG AAAATGTGTA AAAGGCCATG TATTCTTATA

2751 CTAGACTCCT TGAAAGCTGC TTCTGTACGA AACACAGTTC AGAATTTACG

2801 AGAGTATTTA GAGGTAGAGT GGGAAGTTAA ACTAAAAACT CATCGTCAAT

2851 TCAGCAAAAC AAACATGGTG GATCTATGCC CTAAAGTTCC TAAACAGGAC

2901 AATAGCAGTG ATTGTGGAGT ATATTTATTG CAGTATGTGG AAAGCTTCTT

2951 CAAGGATCCT ATTGTTAACT TTGAACTTCC AATTCATTTG GAGAAGTGGT

3001 TTCCTCGTCA TGTAATAAAG ACCAAACGGG AAGATATTCG AGAGCTCATC

3051 TTGAAACTTC ATTTACAGCA ACAGAAGGGC AGCAGTAGCT AGTTAATCTG

3101 TACAAACATG ACACAGATGT TCTCTAAGAT TACTGGAAAG CCCCTTACCA

3151 GCATTTGTGT TAGCCAGCTC ACAGAGAAGA AAATAACTTG CAGTAGTTTT

3201 ATAATAAGTC ATTGGAACAT TATTTAAAAT ATGTAGGACA CATTATTAGA

3251 ATTGTTGGGA TCTCATAGAT GGAATGGGAA TGGGGGTGAT ATAGATAAAC

3301 TTACTAGATA TAAATTAAAA TTTTATAAAT ATTTCATATT TTTCTGAGTA

3351 AATATGATTG GATTATGCAA CAGCATATGT AATATGGGAA TGTTTTGTAG

3401 ATAATAAAAC TTACATGATC TGTACTTCCA CGTGACTGGG TGCTGAGGGG

3451 AGTTAAAGCC TCCCTGGTGC CAGCCCCAGT GCTTGTCAAA TTTGCTGACA

3501 GGTCACATCA TATTGTAATT CTATTCTTTG CAGCTCAAGC ATGCAGTATG

3551 AATACTGTGT ATTTTTTAAA AAAATAATTT AGTATCAAGG CTTCAGAAAA

3601 TGCCATTTAC GGCATCCCTT CTGTATGTAA CAAAAAGACA TTCATAATGT

3651 TAGGAAGATG ATAAAAATTC GCTCTTTTAA AGTGCAGCTT ATTATTCTCA

3701 ATTGCTAAAT ACGATTACTC TGCTTTTTTT TTTTCATTTC TTTTGATGTC

3751 ATATGTGAGT ATCTTATAAT TTAGTTCATT TGTTCAGGGT AAAATTTGAA

3801 ACAAAAAATT TTACCTGTGC AAAATAGTTT TTTAAAAATT ATACATGTAG

3851 CTCAACTTGA GGTACTGCTA TATAAATATT CACTCACATT ATCACGGAAT

3901 TTATGTATAG TTTCTCTAAT ATAGAAGATA AAATTGGTGT CCTCATAACT

3951 TTAACAAAGA AAACCCTCAG TCCTATTTAT TAATGGGTAG AATTAAATAT

4001 ATAATTTTAT AGCTCAGTTT ACCCAGTATT CATCTGCAAA GCCAGATTGC

4051 TCTCATTGCT TTTATATTTT TAAATTGTAG CTTTTAGAGA CCTATGATCC

4101 TCATGGAACT TAATTTTTTA TTAAATATTC AGGTAACAGT TCTGAATTCA

4151 TGTGATAATG GTGGCATTAT ATATGATTAA ACACTTCAGA ACTTTCTAAT

4201 GTTATCAGGA GTATTTTGAG GGAGATATGA TTATATTGTA TTTTCTCAGA

4251 TAAGAAAAAT GTTTTTTAAC AATATTATTT TAATCTGTTT TAAGCATCTC

4301 TTAGATTTAC ATTATAACTA CATAAAGCAG TGAAGCAAAG GCAAATTAAG

4351 ATAAAGCTAG AAAGTCTGAA CATTTTATTT CAAAATCATA CGAATCGGGG

4401 TCAGTTAAGC CTCAGTATTC TTAGCTTTTG TTGATTTTGG CACTATCTTT

4451 ATATTATTAA ATATATTTGT TGTTTGGATA TTTCATATAA AGATGGCTAT

4501 AATTACATAT TTCATTCCCA ATTTGTGTGT GTTGGGGGGT ACTTTTAAAG

4551 GTGACTATTG TTTTGTACAT CTAATTTTGG GAAACCAAGT CTATAAGACA

4601 TCTTGTGATT TCTTAATGTT TTTGTTTGTA TGTTTTTCAA AGATA CACT

4651 GTCCTTTATC ATGTTTTGAA GATTGTTTAA AATTCATTTT CCTAAATTAA

4701 TGTGCAAGTA ATGTTTTGAG GATATCGGTG TTTTATATTA AACATATTTC

4751 CAATTCAAAA AAAAAAAAAA AAAAACTTAT CGATACCGTC GACCTCGATG

4801 ATGATGATGA TGATGATGAT GTCGAC

BLAST Results

No BLAST result

Medline entries

No Medlme entry

Peptide information for frame 3

ORF from 138 bp to 3089 bp; peptide length: 984 Category: similarity to known protein

1 MDKRKLGRRP SSSEIITEGK RKKΞSSDLSE IRKMLNAKPE DVHVQSPLΞK 51 FRSSERWTLP LQWERSLRNK VISLDHKNKK HIRGCPVTSR SSPERIPRVI 101 LTNVLGTELG RKYIRTPPVT EGSLSDTDNL QSEQLSSSSD GSLESYQNLN 151 PHKSCYLSER GSQRSKTVDD NSAKQTAHNK EKRRKDDGIΞ LLISDTQPED 201 LNSGSRGCDH LEQESRNKDV KYSDSKVELT LISRKTKRRL RNNLPDSQYC 251 TSLDKSTEQT KKQEDDSTIS TEFERPSENY HQDPKLPEEI TTKPTKSDFT 301 KLSSLNSQEL TLSNATKSAS AGSTTETVEY ΞNSIDIVGIS SLVEKDENEL 351 NTIEKPILRG HNEGNQSLIS AEPIVVSSDE EGPVEHKSSE ILKLQΞKQDR 401 ETTNENESTS ESALLELPLI TCESVQMSSE LCPYNPVMEN ISSIMPSNEM 451 DLQLDFIFTS VYIGKIKGAS KGCVTITKKY IKIPFQVSLN EISLLVDTTH 501 LKRFGLWKSK DDNHSKRSHA ILFFWVSΞDY LQEIQTQLEH SVLSQQSKSS 551 EFIFLELHNP VSQREELKLK DIMTEISIIS GELELSYPLS WVQAFPLFQN 601 LSSKESSFIH YYCVSTCSFP AGVAVAEEMK LKSVSQPSNT DAAKPTYTFL 651 QKQSSGCYSL SITSNPDEEW REVRHTGLVQ KLIVYPPPPT KGGLGVTNED 701 LECLEEGEFL NDVIIDFYLK YLILEKASDE LVERSHIFSS FFYKCLTRKE 751 NNLTEDNPNL SMAQRRHKRV RTWTRHINIF NKDYIFVPVN ESSHWYLAVI 801 CFPWLEEAVY EDFPQTVSQQ ΞQAQQSQSDN KTIDNDLRTT STLSLSAEDS 851 QSTESNMSVP KKMCKRPCIL ILDSLKAASV RNTVQNLREY LEVEWEVKLK 901 THRQFSKTNM VDLCPKVPKQ DNSSDCGVYL LQYVESFFKD PIVNFELPIH 951 LEK FPRHVI KTKREDIREL ILKLHLQQQK GSSS

BLASTP hits

Entry SPAC17A5_7 from database TREMBL:

"ΞPAC17A5.07c"; product: "hypothetical protein"; S. pombe chromosome I cosmid cl7A5. Schizosaccharomyces pombe (fission yeast)

Length = 652

Score = 275 (96.8 bits). Expect = 1.9e-29, Sum P(3) = 1.9e-29

Identities = 56/120 (46%), Positives = 78/120 (65%)

Entry S49947 from database PIR:

SMT4 protein - yeast (Ξaccharomyces cerevisiae)

Length = 1034

Score = 163 (57.4 bits), Expect = 4.6e-16, Sum P(3) = 4.6e-16

Identities = 46/159 (28%), Positives = 76/159 (47%)

Entry YQG6_CAEEL from database SWISSPROT:

HYPOTHETICAL 35.7 KD PROTEIN C41C4.6 IN CHROMOSOME II.

Length = 342

Score = 162 (57.0 bits), Expect = 6.1e-13, Sum P(3) = 6.1e-13

Identities = 37/119 (31%), Positives = 62/119 (52%)

Entry AB018340_1 from database TREMBL: gene: "KIAA0797"; product: "KIAA0797 protein"; Homo sapiens mRNA for

KIAA0797 protein, partial eds.

Score = 540, P = 1.9e-50, identities = 120/243, positives = 155/243

Alert BLASTP hits for DKFZphfbr2_16gl8, frame 3

TREMBL :ATT16L1_11 gene: "T16L1.110"; product: "putative protein"; Arabidopsis thaliana DNA chromosome 4, BAC clone T16L1 (ESSAII project), N = 2, Score = 239, P = 2.1e-18

>TREMBL:ATT16L1_11 gene: "T16L1.110"; product: "putative protein";

Arabidopsis thaliana DNA chromosome 4, BAC clone T16L1 (ESSAII project) Length = 710

HSPs:

Score = 239 (35.9 bits), Expect = 2.1e-18, Sum P(2) = 2.1e-18 Identities = 51/135 (37%), Positives = 78/135 (57%)

Query: 683 IVYPPPPTKGGLGVTNEDLECLEEGEFLNDVIIDFYLKYLILEKASDELVERSHIFSSFF 742

+VYP + V +D+E L+ F+ND IIDFY+KYL + S + R H F+ FF Ξbjct: 176 LVYPQGEPDAVV-VRKQDIELLKPRRFINDTIIDFYIKYL-KNRISPKERGRFHFFNCFF 233

Query: 743 YKCLTRKENNLTEDNPNLSMAQRRHKRVRTWTRHINIFNKDYIFVPVNESSHWYLAVICF 802

+ RK NL + P+ + ++RV+ WT+++++F KDYIF+P+N S HW L +IC Sb ct: 234 F RKLANLDKGTPSTCGGREAYQRVQKWTKNVDLFEKDYIFIPINCSFHWSLVIICH 289

Query: 803 PWLEEAVYEDFPQTV 817

P + + PQ V Sbjct: 290 PGELVPSHVENPQRV 304

Score = 70 (10.5 bits), Expect = 2.1e-18, Sum P(2) = 2.1e-18 Identities = 13/28 (46%), Positives = 15/28 (53%)

Query: 948 PIHLEKWFPRHVIKTKREDIRELILKLH 975

P HL FP KR +1 EL+ LH Sbjct: 403 PSHLRNWFPAKEASLKRRNILELLYNLH 430

Pedant information for DKFZphfbr2_16gl8, frame 3

Report for DKFZphfbr2_16gl8.3 [LENGTH] 984 [MW] 112265.80

[pi] 6.13

[HOMOL] TREMBL:AB018340_1 gene: "KIAA0797"; product: "KIAA0797 protein"; Homo sapiens mRNA for KIAA0797 protein, partial eds. 8e-53

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YIL031w] 9e-17 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YPL020c] 4e-06 [BLOCKS] BL00494C Bacterial luciferase subunits proteins [PROSITE] AMIDATION 3 [PROSITE] MYRISTYL 9 [PROSITE] CAMP PH0SPH0 SITE 2 [PROSITE] CK2 PHOSPHO SITE 30 [PROSITE] TYR PHOSPHO SITE 1 [PROSITE] PKC PHOSPHO SITE 19 [PROSITE] ASN GLYCOSYLATION 12 [KW] Alpha Beta [KW] LOW COMPLEXITY 4.47 %

SEQ MDKRKLGRRPSSSEIITEGKRKKSSSDLSEIRKMLNAKPEDVHVQSPLSKFRSSERWTLP SEG

PRD ccccceeecccceeeeecccccccccchhhhhhhhhhccccccccccccccccccccchh

SEQ LQWERSLRNKVISLDHKNKKHIRGCPVTSRΞSPERIPRVILTNVLGTELGRKYIRTPPVT SEG

PRD hhhhhhhhhheeeeccccceeeccccccccccccceeeeeeeeeccceeeccceeecccc

SEQ EGSLSDTDNLQSEQLSSSSDGSLESYQNLNPHKSCYLSERGSQRSKTVDDNSAKQTAHNK SEG xxxxxxxxxxxxxxxx

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccchhhhhhhh

SEQ EKRRKDDGISLLISDTQPEDLNSGSRGCDHLEQESRNKDVKYSDΞKVELTLISRKTKRRL SEG

PRD hhhhcccceeeeecccccccccccccccccccccccccccccccccceeeeeehhhhhhh

SEQ RNNLPDSQYCTSLDKSTEQTKKQEDDSTISTEFERPSENYHQDPKLPEEITTKPTKSDFT SEG

PRD hccccccccccccccccchhhhhccccccccccccccccccccccccccccccccccccc

SEQ KLSSLNSQELTLSNATKSASAGSTTETVEYSNSIDIVGISSLVEKDENELNTIEKPILRG SEG

PRD ccccccccceeehhhhhhhcccccceeeeccceeeceeeccchhhhhhhhhhhccccccc

SEQ HNEGNQSLISAEPIVVSSDEEGPVEHKSSEILKLQSKQDRETTNENESTSESALLELPLI

SEG xxxxxxxxxxxxxxxxx ...

PRD cccccceeeecceeeeecccccccccchhhhhhhhhhhhhhcccccccchhhhhccccce

SEQ TCESVQMSSELCPYNPVMENISΞIMPSNEMDLQLDFIFTSVYIGKIKGASKGCVTITKKY SEG

PRD eecccccccccccccccccceeeccccchhhhhhheeeeeeeeeeeeccccceeeeeeee

SEQ IKIPFQVSLNEISLLVDTTHLKRFGLWKSKDDNHSKRSHAILFFWVSSDYLQEIQTQLEH SEG

PRD eeeeccccceeeeeeecccceeeeeeeecccccccccceeeeeeeeccchhhhhhhhhhh

SEQ SVLSQQSKSSEFIFLELHNPVSQREELKLKDIMTEISIISGELELSYPLSWVQAFPLFQN SEG

PRD hhhhccccceeeeeeeeccccccchhhhhhhhhheeeeeccceeeeccceeeeeeceeec

SEQ LSSKESSFIHYYCVSTCSFPAGVAVAEEMKLKSVSQPSNTDAAKPTYTFLQKQSSGCYSL SEG

PRD ccccccccceeeeecccccccchhhhhhhhhhhcccccccccccccceeeecccccccce

SEQ SITSNPDEEWREVRHTGLVQKLIVYPPPPTKGGLGVTNEDLECLEEGEFLNDVIIDFYLK SEG :

PRD eeccccccceeeeeeccceeeeeeecccccccccccccchhhhhhhhccchhhhhhhhhh

SEQ YLILEKASDELVERSHIFSSFFYKCLTRKENNLTEDNPNLSMAQRRHKRVRTWTRHINIF SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccchhhhhhhhhhhhhhhhhhhhc

SEQ NKDYIFVPVNESSHWYLAVICFPWLEEAVYEDFPQTVSQQSQAQQSQSDNKTIDNDLRTT SEG xxxxxxxxxxx

PRD cceeeeeccccccceeeeeeeccchhhhhhhccccchhhhhhhhhhcccccccccccccc

SEQ STLSLSAEDSQSTEΞNMSVPKKMCKRPCILILDSLKAASVRNTVQNLREYLEVEWEVKLK SEG

PRD cceeeeecccccceeeccccccccccceeeeeccccccccchhhhhhhhhhhhhhhhhhh

SEQ THRQFSKTNMVDLCPKVPKQDNSSDCGVYLLQYVESFFKDPIVNFELPIHLEKWFPRHVI SEG PRD hhhhhccccccccccccccccccccceeeeehhhhhhhcccceeecccccccccccchhh

SEQ KTKREDIRELILKLHLQQQKGSSS SEG PRD hhhhhhhhhhhhhhhhhhhccccc

Prosite for DKFZphfbr2_16gl8.3

PS00001 314->318 ASN_GLYCOSYLATION PDOC00001 PS00001 365->369 ASNJΞLYCOSYLATION PDOC00001 PS00001 406->410 ASN_GL COSYLATION PDOC00001 PS00001 440->444 ASN_GLYCOSYLATION PDOC00001 PΞ00001 513->517 ASN_GLYCOSYLATION PDOC00001 PS00001 600->604 ASN_GLYCOSYLATION PDOC00001 PS00001 752->756 ASN_GLYCOSYLATION PDOC00001 PS00001 759->763 ASN_GLYCOSYLATION PDOC00001 PS00001 790->794 ASN_GLYCOSYLATION PDOC00001 PS00001 830->834 ASN_GLYCOSYLATION PDOC00001 PSOOOOl 856->860 ASN_GLYCOSYLATION PDOC00001 PS00001 922->926 ASN 3LYCOSYLATION PDOC00001 PS00004 8->12 CAMP_PHOSPHO_SITE PDOC00004 PS00004 21->25 CAMP_PHOSPHO_SITE PDOC00004 PS00005 54->57 PKC_PHOSPHO_SITE PDOC00005 PS00005 66->69 PKC_PHOSPHO_SITE PDOC00005 PS00005 88->91 PKC_PHOSPHO_SITE PDOC00005 PS00005 158->161 PKC_PHOSPHO_SITE PDOC00005 PS00005 162->165 PKC_PHOSPHO_SITE PDOC00005 PS00005 172->175 PKC_PHOSPHO_SITE PDOC00005 PS00005 233->236 PKC_PHOSPHO_SITE PDOC00005 PS00005 236->239 PKC_PHOSPHO_SITE PDOC00005 PS00005 260->263 PKC_PHOSPHO_SITE PDOC00005 PS00005 291->294 PKC_PHOSPHO_SITE PDOC00005 PS00005 477->480 PKC_PHOSPHO_SITE PDOC00005 PS00005 515->518 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 562->565 PKC_PHOSPHO_SITE PDOC00005 PS00005 602->605 PKC_PHOSPHO_SITE PDOC00005 PS00005 747->750 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 874->877 PKC_PHOSPHO_SITE PDOC00005 PS00005 879->882 PKC_PHOSPHO_SITE PDOC00005 PS00005 901->904 PKC_PHOSPHO_SITE PDOC00005 PS00005 962->965 PKC_PHOSPHO_SITE PDOC00005 PS00006 11->15 CK2_PHOSPHO_SITE PDOC00006 PS00006 24->28 CK2_PHOSPHO_SITE PDOC00006 PS00006 91->95 CK2_PHOSPHO_SITE PDOC00006 PS00006 123-M27 CK2_PHOSPHO_SITE PDOC00006 PS00006 125->129 CK2_PHOSPHO_SITE PDOC00006 PS00006 137->141 CK2_PHOSPHO_SITE PDOC00006 PS00006 167->171 CK2_PHOSPHO_SITE PDOC00006 PS00006 196->200 CK2_PHOSPHO_SITE PDOC00006 PS00006 225->229 CK2_PHOSPHO_SITE PDOC00006 PS00006 251->255 CK2_PHOSPHO_SITE PDOC00006 PS00006 271->275 CK2_PHOΞPHO_SITE PDOC00006 PS00006 295->₂99 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 323->327 CK2_PHOSPHO_SITE PDOC00006 PS00006 341->345 CK2_PHOSPHO_SITE PDOC00006 PS00006 377->381 CK2_PHOSPHO_SITE PDOC00006 PS00006 396->400 CK2_PHOSPHO_SITE PDOC00006 PS00006 402->406 CK2_PHOSPHO_SITE PDOC00006 PS00006 408->412 CK2_PHOSPHO_SITE PDOC00006 PS00006 488->492 CK2_PHOSPHO_SITE PDOC00006 PS00006 509->513 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 536->540 CK2_PHOSPHO_SITE PDOC00006 PS00006 562->566 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 602->606 CK2_PHOSPHO_SITE PDOC00006 PS00006 638->642 CK2_PHOSPHO_SITE PDOC00006 PS00006 664->668 CK2_PHOSPHO_SITE PDOC00006 PS00006 697->701 CK2_PHOSPHO_SITE PDOC00006 PS00006 747->751 CK2_PHOSPHO_SITE PDOC00006 PS00006 826->830 CK2_PHOSPHO_SITE PDOC00006 PS00006 846->850 CK2_PHOSPHO_SITE PDOC00006 PS00006 962->966 CK2_PHOSPHO_SITE PDOC00006 PS00007 216->223 TYR_PHOSPHO_SITE PDOC00007 PS00008 84->90 MYRISTYL PDOC00008 PS00008 106-M12 MYRISTYL PDOC00008 PS00008 141->147 MYRISTYL PDOC00008 PS00008 161-M67 MYRISTYL PDOC00008 PS00008 204->210 MYRISTYL PDOC00008 PS00008 468->474 MYRISTYL PDOC00008 PS00008 505->511 MYRISTYL PDOC00008 PS00008 622->628 MYRISTYL PDOC00008 PS00008 693->699 MYRISTYL PDOC00008 PS00009 6->10 AMIDATION PDOC00009 PS00009 18->22 AMIDATION PDOC00009 PS00009 109->113 AMIDATION PDOC00009

(No Pfam data available for DKFZphfbr2_16gl8.3)

DKFZphfbr2_16ιl2

group: transmembrane protein

DKFZphfbr2_16ιl2 encodes a novel 185 amino acid protein, with strong similarity to PUT2 protein of Fugu rubripes.

The novel protein contains 1 transmembrane region.

PUT 2 is a Fugu rupies protein similar to the neural cell adhesion molecule LI (Ll-CAM) a mitosis-specific chromosome segregation protein (SMCl) and the calcium channel alpha-1 subunit homolog (CCA1) .

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of bram-specific genes and as a new marker for neuronal cells. strong similarity to Fugu rubripes PUT2 complete cDNA, complete eds, EST hits, TRANSMEMBRANE 1

Sequenced by LMU

Locus: /map="873.3/875.1 CR from top of Chrl linkage group"

Insert length: 1552 bp

Poly A stretch at pos. 1528, polyadenylation signal at pos. 1506

1 GGGGGGGGAC AACTGGGTCT TTTGCGGCTG CAGCGGGCTT GTAGGCGTCC 51 GGCTTTGCTG GCCCAGCAAG CCTGATAAGC ATGAAGCTCT TATCTTTGGT

101 GGCTGTGGTC GGGTGTTTGC TGGTGCCCCC AGCTGAAGCC AACAAGAGTT

151 CTGAAGATAT CCGGTGCAAA TGCATCTGTC CACCTTATAG AAACATCAGT

201 GGGCACATTT ACAACCAGAA TGTATCCCAG AAGGACTGTT GTAGCAACTG

251 CCTGCACGTG GTGGAGCCCA TGCCAGTGCC TGGCCATGAC GTGGAGGCCT

301 ACTGCCTGCT GTGCGAGTGC AGGTACGAGG AGCGCAGCAC CACCACCATC

351 AAGGTCATCA TTGTCATCTA CCTGTCCGTG GTGGGTGCCC TGTTGCTCTA

401 CATGGCCTTC CTGATGCTGG TGGACCCTCT GATCCGAAAG CCGGATGCAT

451 ACACTGAGCA ACTGCACAAT GAGGAGGAGA ATGAGGATGC TCGCTCTATG

501 GCAGCAGCTG CTGCATCCCT CGGGGGACCC CGAGCAAACA CAGTCCTGGA

551 GCGTGTGGAA GGTGCCCAGC AGCGGTGGAA GCTGCAGGTG CAGGAGCAGC

601 GGAAGACAGT CTTCGATCGG CACAAGATGC TCAGCTAGAT GGGCTGGTGT

651 GGTTGGGTCA AGGCCCCAAC ACCATGGCTG CCAGCTTCCA GGCTGGACAA

701 AGCAGGGGGC TACTTCTCCC TTCCCTCGGT TCCAGTCTTC CCTTTAAAAG

751 CCTGTGGCAT TTTTCCTCCT TCTCCCTAAC TTTAGAAATG TTGTACTTGG

801 CTATTTTGAT TAGGGAAGAG GGATGTGGTC TCTGATCTCT GTTGTCTTCT

851 TGGGTCTTTG GGGTTGAAGG GAGGGGGAAG GCAGGCCAGA AGGGAATGGA

901 GACATTCGAG GCGGCCTCAG GAGTGGATGC GATCTGTCTC TCCTGGCTCC

951 ACTCTTGCCG CCTTCCAGCT CTGAGTCTTG GGAATGTTGT TACCCTTGGA 1001 AGATAAAGCT GGGTCTTCAG GAACTCAGTG TTTGGGAGGA AAGCATGGCC 1051 CAGCATTCAG CATGTGTTCC TTTCTGCAGT GGTTCTTATC ACCACCTCCC 1101 TCCCAGCCCC AGCGCCTCAG CCCCAGCCCC AGCTCCAGCC CTGAGGACAG 1151 CTCTGATGGG AGAGCTGGGC CCCCTGAGCC CACTGGGTCT TCAGGGTGCA 1201 CTGGAAGCTG GTGTTCGCTG TCCCCTGTGC ACTTCTCGCA CTGGGGCATG 1251 GAGTGCCCAT GCATACTCTG CTGCCGGTCC CCTCACCTGC ACTTGAGGGG 1301 TCTGGGCAGT CCCTCCTCTC CCCAGTGTCC ACAGTCACTG AGCCAGACGG 1351 TCGGTTGGAA CATGAGACTC GAGGCTGAGC GTGGATCTGA ACACCACAGC 1401 CCCTGTACTT GGGTTGCCTC TTGTCCCTGA ACTTCGTTGT ACCAGTGCAT 1451 GGAGAGAAAA TTTTGTCCTC TTGTCTTAGA GTTGTGTGTA AATCAAGGAA 1501 GCCATCATTA AATTGTTTTA TTTCTCTCAA AAAAAAAAAA AAAAAAAATA 1551 TC

BLAST Results

Entry HS808349 from database EMBL: human STS WI-11986. Score = 1716, P = 5.7e-73, identities = 364/378

Entry HS487355 from database EMBL: human STS WI-13088. Score = 1358, P = 1.3e-56, identities = 274/277

Medl e entries No Medline entry

Peptide information for frame 3

ORF from 81 bp to 635 bp; peptide length: 185 Category: similarity to unknown protein

1 MKLLSLVAVV GCLLVPPAEA NKSSEDIRCK CICPPYRNIS GHIYNQNVSQ

51 KDCCSNCLHV VEPMPVPGHD VEAYCLLCEC RYEERSTTTI KVIIVIYLSV

101 VGALLLYMAF LMLVDPLIRK PDAYTEQLHN EEENEDARSM AAAAASLGGP

151 RANTVLERVE GAQQRWKLQV QEQRKTVFDR HKMLS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_16ιl2, frame 3

TREMBL:AF026198_5 gene: "PUT2"; product: "putative protein 2"; Fugu rubripes neural cell adhesion molecule LI homolog (Ll-CAM) gene, complete eds; putative protein 1 (PUTl) gene, partial eds; mitosis-specific chromosome segregation protein SMCl homolog (SMCl) gene, complete eds; and calcium channel alpha-1 subunit homolog (CCAl) and putative protein 2 (PUT2) genes, partial eds, complete sequence., N = 1, Score = 655, P = 2.8e-64

TREMBL :CER12C12_5 gene: "R12C12.6"; Caenorhabditis elegans cosmid R12C12., N = 1, Score = 225, P = le-18

>TREMBL:AF026198_5 gene: "PUT2"; product: "putative protein 2"; Fugu rubripes neural cell adhesion molecule LI homolog (Ll-CAM) gene, complete eds; putative protein 1 (PUTl) gene, partial eds; mitosis-specific chromosome segregation protein SMCl homolog (SMCl) gene, complete eds; and calcium channel alpha-1 subunit homolog (CCAl) and putative protein 2 (PUT2) genes, partial eds, complete sequence. Length = 187

HSPs:

Score = 655 (98.3 bits), Expect = 2.8e-64, P = 2.8e-64 Identities = 124/163 (76%), Positives = 140/163 (85%)

Query: 22 KSSEDIRCKCICPPYRNISGHIYNQNVSQKDCCSNCLHVVEPMPVPGHDVEAYCLLCECR 81

KS +D+RCKCICPPYRNISGHIYN+N +QKDC NCLHVV+PMPVPG+DVEAYCLLCEC+ Sbjct: 31 KSFDDVRCKCICPPYRNISGHIYNRNFTQKDC—NCLHVVDPMPVPGNDVEAYCLLCECK 88

Query: 82 YEERSTTTIKVIIVIYLSVVGALLLYMAFLMLVDPLIRKPDAYTEQLHNEEENEDARSMA 141

YEERST TI+V I+I+LSVVGALLLYM FL+LVDPLIRKPD + LHNEE++ED + Sbjct: 89 YEERSTNTIRVTIIIFLSVVGALLLYMLFLLLVDPLIRKPDPLAQTLHNEEDSEDIQPQM 148

Query: 142 AAAASLGGP-RANTVLERVEGAQQRWKLQVQEQRKTVFDRHKML 184

+ G P R NTVLERVEGAQQRWK QVQEQRKTVFDRHKML Sbjct: 149 S GDPARGNTVLERVEGAQQRWKKQVQEQRKTVFDRHKML 187

Pedant information for DKFZphfbr2_16ιl2, frame 3

Report for DKFZphfbr2_16ιl2.3

[LENGTH] 185 [MW] 20764.29 [pi] 6.21 [HOMOL] TREMBL:AF026198_5 gene: "PUT2"; product: "putative protein 2' Fugu rubripes neural cell adhesion molecule LI homolog (Ll-CAM) gene, complete eds; putative protein 1 (PUTl) gene, partial eds; mitosis-speeific chromosome segregation protein SMCl homolog (SMCl) gene, complete eds; and calcium channel alpha-1 subunit homolog (CCAl) and putative protein 2 (PUT2) genes, partial eds, complete sequence. 3e-68 [PROSITE] MYRISTYL 1 [PROSITE] CK2_PHOSPHO_SITE 4 [PROSITE] PKC_PHOSPHO_SITE 2 [PROSITE] ASN_GLYCOSYLATION 3 [KW] SIGNAL PEPTIDE 21 [KW] TRANSMEMBRANE 1 [KW] LOW COMPLEXITY 2.70

SEQ MKLLSLVAWGCLLVPPAEANKΞSEDIRCKCICPPYRNISGHIYNQNVSQKDCCSNCLHV SEG PRD ccceeeeeeeeccccccccccccccceeeeeecccccccccceeeccccccccccceeee MEM

SEQ VEPMPVPGHDVEAYCLLCECRYEERSTTTIKVIIVI LSVVGALLLYMAFLMLVDPLIRK SEG PRD eecccccccccchhhhhhhhhhhhccccceeeeeeehhhhhhhhhhhhhhhhhhhccccc

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM ...

SEQ PDAYTEQLHNEEENEDARSMAAAAASLGGPRANTVLERVEGAQQRWKLQVQEQRKTVFDR SEG xxxxx PRD ccchhhhhhhhhcccchhhhhhhhhhccccccchhhhhhhchhhhhhhhhhhhhhhhhhh MEM

SEQ HKMLS SEG PRD hhccc MEM

Prosite for DKFZphfbr2_16ιl2.3

PS00001 21->25 ASN_GLYCOSYLATION PDOC00001

PS00001 38->42 ASN_GLYCOSYLATION PDOC00001

PS00001 47->51 ASN_GLYCOΞYLATION PDOC00001

PS00005 49->52 PKC_PHOSPHO_ΞITE PDOC00005

PS00005 89->92 PKC_PHOSPHO_SITE PDOC00005

PS00006 23->27 CK2_PHOSPHO_SITE PDOC00006

PS00006 49->53 CK2_PHOSPHO_SITE PDOC00006

PS00006 154->158 CK2_PHOSPHO_SITE PDOC00006

PS00006 176->180 CK2_PHOSPHO_SITE PDOC00006

PS00008 148->154 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2 16ιl2.3)

DKFZphfbr2_16k22

group: brain derived

DKFZphfbr2_16k22 encodes a novel 108 amino acid protein with very weak similarity to thioredoxin of Bacillus subtilis.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . weak similarity to thioredoxin complete cDNA, complete eds, genomic DNA?

Sequenced by BMFZ

Locus : unknown

Insert length: 2088 bp

Poly A stretch at pos. 2065, no polyadenylation signal found

1 AAAAGGAAGA AGGAAATAAG GATATTTCAA GGGTTACCAA AGTCGAGGAA 51 AACTATTTTA AGAAGAAATC TGAATTATTT GTGCACATAG GTTGTAATAA

101 TAGCATCTTG CATTAAATGG TGTTTTCTAG CTTACAAAGT GGATTCATAT

151 ACACTATTGT AACTGACTCT CTACAAACTT GCAAGGTTAG CAAGACAAAT

201 GGTATTTTAA GATAACAAAC TGAGACTCAA AAAAGGCAAG TAACTCGTTC

251 TACTTCCCAA AGCCAGAAAG TGGCAAAATA GAAAATGGAT CCTGAATCTC

301 CAACACCATG CAAACTAAGA GAGGGAATCC TCTGTAGAGG GAATGGAAGT

351 AAAAAGGCAC AAGTGGTGAT GTCACCTTCT GAACAGAGAT GGAACTTTTC

401 TTCCTCTGAG AAAAAAGAGA AAAGATAGTT TTAAGTGGCA AAAGAACATG

451 AAGCAATGTG AGGTGAAGAA ACAGAAAAGA CTATGGATGG AATTCCTAGA

501 TGTGAGATAC ACAAAGTTCC ATTTCAAAGA GAAATATCTA TAGATAGGCA

551 TAAAGTTACA CACCTGAACT ACCAACTCTG AACCAGTAAC TCAAGAGATA

601 TTTTGTGTGT CCCACAAGCC ATATGGCTCT GGGGACAAAT TATCTGAAAG

651 TGCCCAATAA GAAAAATATT TGAGGAAGGG GAGTTGGTGA GTGAATGAAT

701 TAAAGGACAT CAGAAAGATA CATTGACTGT TCTCCTTCCC AGGAAACAAA

751 GTGGCTAAGT CAAAACAACG GGCAGCTGTG GGATAGCAAA GAAAAAAAAA

801 CTTCCAGGCC CAGGTTCTAG TGAAAGCTAC TATGGAAGTT AGCCACTCAA

851 CTTTAGAACC AGAGGCTTCT TTTCCTCCTC CCTTCTTATC TTTTCTAGTT

901 TATAGCAAAT TTATATTGAG CCACTTATTC TTTCTGAATG CTAGTTCCCC

951 TTTAGCATTT CTTTTTCTTC ATTCCCTTTG GACTGGCCCA ATGCTTTGGC 1001 CCCTTATCAA AGCATTTTCT AAGAAACAGT CTGACAGCTC TAATTTGCAT 1051 CTGGTTATGC AAGATGTGGT TAAGAACATG GACTCTGGAG GTAAATACAC 1101 CTTGATTCCA ATTCATTCTC TCATTTATTC ATTCAGCAAA TATTTAGTGA 1151 ACATCTAACA TGTGCTAGGC ACTGTTCTAG TTGCTGAGGA TACAGCTTCA 1201 AACAAAATAA GGTCTCTGCA AGGATGCCTT CTCTTACCAC TCCTATTCAG 1251 CGTAGTATTG GAAGTCCTGG CCAGGGCAAT CAGGCAAGAA AAAGAAATCA 1301 AGGTCATCCA AATAGGAAGA GAGGAAGTCA AACTATCCCT GTTTACAGAC 1351 AACATGATCC TACATCTAGA AAAAAACCCA TTGTCTTAGC CCAAAAGCTT 1401 CTTAGGCTGA TAAACAACTT CAGCAAAGTC TTAGGATACA AAATCCATGT 1451 GCAAAAAACA CTAGCATTCT TATACACCAA CAACAGTCAA GCCGAGATCC 1501 AAATCAGGAA CAAACTCCTA TTCACAATTG CCACAAAAAC AATAGAACAG 1551 GAAAACAGCT AACTAGGAAG GTGAAAGATC TCTACAAGGA GAACTACAAA 1601 CCACTGCTCA CAGAAATCAG AGATGACACA TATAAATGGA AAAACATTCC 1651 ATGATCATGG ATAGGAAGAA TGAATATTAC TGAAATGGCT ATACTGTCCA 1701 AAGCAATTTA TAGATTCAAT GCTATTCCTA GTAAACTACC ATTGAGATTT 1751 TTTACAGAAC TAGAAAAAAA AAAAACTATT TTAAGGCTGG GCGCAGTGGC 1801 TCTCACCTGT AATCCCAGCA CTTTGGGAGG CCGAGATGGG TGGATCACGA 1851 GGTCAGGAGA TGGAAAACAT CCTGGCTAAC ATGGTGAAAC CCCGTCTCTA 1901 CTAAAAATAC AAAAAATTAG CCAGGCGTGG TGGTGGGCGC CTGTAATCCC 1951 AGCTGCTCGG GAGGCTGAGG CAGGATAATG GTGTGAACCC GGGAGGCAGA 2001 GCTTGCAGTG AGCTGAGATT GCACCACTGC ACTCCAGCCT GAGGGACAGA 2051 GTGAGACTCC ATCTCAAAAA AAAAAAAAAA AAAAAAAA

BLAST Results o BLAST result

Medline entries No Medlme entry

Peptide information for frame 1

ORF from 832 bp to 1155 bp; peptide length: 108 Category: putative protein

1 MEVSHSTLEP EAΞFPPPFLS FLVYSKFILΞ HLFFLNASSP LAFLFLHSLW 51 TGPMLWPLIK AFSKKQSDSS NLHLVMQDVV KNMDSGGKYT LIPIHSLIYS 101 FSKYLVNI

BLASTP hits

Entry B37192 from database PIR: thioredoxin - Bacillus subtilis Score = 71 (25.0 bits), Expect = 0.040, P = 0.039 Identities = 16/49 (32%), Positives = 30/49 (61%)

Alert BLASTP hits for DKFZphfbr2_16k22, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_16k22, frame 1

Report for DKFZphfbr2_16k22.1

[LENGTH] 108

[MW] 12281.47

[pi] 8.06

[PROSITE] MYRISTYL 1

[PROSITE] CAMP_PHOΞPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 1

[PROSITE] ASN_GLYCOSYLATION 1

[KW] Alpha_Beta

SEQ MEVSHSTLEPEASFPPPFLSFLVYSKFILSHLFFLNASSPLAFLFLHSLWTGPMLWPLIK

PRD ccccccccccccccccccchhhhhhhhhhhhhhhhccccchhhhhhhhccccccchhhhh

SEQ AFSKKQSDSSNLHLVMQDVVKNMDΞGGKYTLIPIHSLIYSFSKYLVNI

PRD hhhcccccccceeehhhhhhcccccccceeeeeccceeeecccccccc

Prosite for DKFZphfbr2 16k22.1

PS00001 36- ->40 ASN GLYCOSYLATION PDOC00001

PS00004 64- ->68 CAMP PHOSPHO SITE PDOC00004

PS00005 63- ->66 PKC PHOSPHO SITE PDOC00005

PS00006 6- ->10 CK2 PHOSPHO SITE PDOC00006

PS00008 86- ->92 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_16k22.1) DKFZphfbr2_16112

group: transmembrane protein

DKFZphfbr2_16112 encodes a novel 267 amino acid protein with similarity to gallus gallus putative transmembrane protein E3-16

The novel protein contains one putative transmembrane domain. In chicken, E3-16 is expressed specifically in the inner ear.

No informative BLAST results; no predictive prosite, pfam or SCOP motife

The new protein can find application in studying the expression profile of brain-specific genes and as a new marker for neurons involved in perception of hearing. similarity to gallus putative transmembrane protein E3-16 complete cDNA, complete eds, EST hits potental start at Bp 73 matchs kozak consensus PyCCataG

TRANSMEMBRANE 1

Sequenced by Qiagen

Locus : unknown

Insert length: 2042 bp

Poly A stretch at pos. 2024, polyadenylation signal at pos. 2003

1 GGGGGCGGCG GAGGCAGAGA CCGAGGCTGC ACCGGCAGAG GCTGCGGGGC

51 GGACGCGCGG GCCGGCGCAG CCATGGTGAA GATTAGCTTC CAGCCCGCCG

101 TGGCTGGCAT CAAGGGCGAC AAGGCTGACA AGGCGTCGGC GTCGGCCCCT

151 GCGCCGGCCT CGGCCACCGA GATCCTGCTG ACGCCGGCTA GGGAGGAGCA

201 GCCCCCACAA CATCGATCCA AGAGGGGGGG CTCAGTGGGC GGCGTGTGCT

251 ACCTGTCGAT GGGCATGGTC GTGCTGCTCA TGGGCCTCGT GTTCGCCTCT

301 GTCTACATCT ACAGATACTT CTTCCTTGCG CAGCTGGCCC GAGATAACTT

351 CTTCCGCTGT GGTGTGCTGT ATGAGGACTC CCTGTCCTCC CAGGTCCGGA

401 CTCAGATGGA GCTGGAAGAG GATGTGAAAA TCTACCTCGA CGAGAACTAC

451 GAGCGCATCA ACGTGCCTGT GCCCCAGTTT GGCGGCGGTG ACCCTGCAGA

501 CATCATCCAT GACTTCCAGC GGGGTCTGAC TGCGTACCAT GATATCTCCC

551 TGGACAAGTG CTATGTCATC GAACTCAACA CCACCATTGT GCTGCCCCCT

601 CGCAACTTCT GGGAGCTCCT CATGAACGTG AAGAGGGGGA CCTACCTGCC

651 GCAGACGTAC ATCATCCAGG AGGAGATGGT GGTCACGGAG CATGTCAGTG

701 ACAAGGAGGC CCTGGGGTCC TTCATCTACC ACCTGTGCAA CGGGAAAGAC

751 ACCTACCGGC TCCGGCGCCG GGCAACGCGG AGGCGGATCA ACAAGCGTGG

801 GGCCAAGAAC TGCAATGCCA TCCGCCACTT CGAGAACACC TTCGTGGTGG

851 AGACGCTCAT CTGCGGGGTG GTGTGAGGCC CTCCTCCCCC AGAACCCCCT

901 GCCGTGTTCC TCTTTTCTTC TTTCCGGCTG CTCTCTGGCC CTCCTCCTTC

951 CCCCTGCTTA GCTTGTACTT TGGACGCGTT TCTATAGAGG TGACATGTCT

1001 CTCCATTCCT CTCCAACCCT GCCCACCTCC CTGTACCAGA GCTGTGATCT

1051 CTCGGTGGGG GGCCCATCTC TGCTGACCTG GGTGTGGCGG AGGGAGAGGC

1101 GATGCTGCAA AGTGTTTTCT GTGTCCCACT GTCTTGAAGC TGGGCCTGCC

1151 AAAGCCTGGG CCCACAGCTG CACCGGCAGC CCAAGGGGAA GGACCGGTTG

1201 GGGGAGCCGG GCATGTGAGG CCCTGGGCAA GGGGATGGGG CTGTGGGGGC

1251 GGGGCGGCAT GGGCTTCAGA AGTATCTGCA CAATTAGAAA AGTCCTCAGA

1301 AGCTTTTTCT TGGAGGGTAC ACTTTCTTCA CTGTCCCTAT TCCTAGACCT

1351 GGGGCTTGAG CTGAGGATGG GACGATGTGC CCAGGGAGGG ACCCACCAGA

1401 GCACAAGAGA AGGTGGCTAC CTGGGGGTGT CCCAGGGACT CTGTCAGTGC

1451 CTTCAGCCCA CCAGCAGGAG CTTGGAGTTT GGGGAGTGGG GATGAGTCCG

1501 TCAAGCACAA CTGTTCTCTG AGTGGAACCA AAGAAGCAAG GAGCTAGGAC

1551 CCCCAGTCCT GCCCCCCAGG AGCACAAGCA GGGTCCCCTC AGTCAAGGCA

1601 GTGGGATGGG CGGCTGAGGA ACGGGGCAGG CAAGGTCACT GCTCAGTCAC

1651 GTCCACGGGG GACGAGCCGT GGGTTCTGCT GAGTAGGTGG AGCTCATTGC

1701 TTTCTCCAAG CTTGGAACTG TTTTGAAAGA TAACACAGAG GGAAAGGGAG

1751 AGCCACCTGG TACTTGTCCA CCCTGCCTCC TCTGTTCTGA AATTCCATCC

1801 CCCTCAGCTT AGGGGAATGC ACCTTTTTCC CTTTCCTTCT CACTTTTGCA

1851 TGTTTTTACT GATCATTCGA TATGCTAACC GTTCTCAGCC CTGAGCCTTG

1901 GAGAGGAGGG CTGTAACGCC TTCAGTCAGT CTCTGGGGAT GAAACTCTTA

1951 AATGCTTTGT ATATTTTCTC AATTAGATCT CTTTTCAGAA GTGTCTATAG

2001 AACAATAAAA ATCTTTTACT TCTGAAAAAA AAAAAAAAAA AA

BLAST Results o BLAST result Medlme entries

96325063:

Isolation of markers for chondro-osteogenic differentiation using cDNA library subtraction. Molecular cloning and characterization of a gene belonging to a novel multigene family of integral membrane proteins.

Peptide information for frame 1

ORF from 73 bp to 873 bp; peptide length: 267 Category: similarity to known protein

1 MVKISFQPAV AGIKGDKADK ASASAPAPAS ATEILLTPAR EEQPPQHRSK

51 RGGSVGGVCY LSMGMVVLLM GLVFASVYIY RYFFLAQLAR DNFFRCGVLY

101 EDSLSSQVRT QMELEEDVKI YLDENYERIN VPVPQFGGGD PADIIHDFQR

151 GLTAYHDISL DKCYVIELNT TIVLPPRNFW ELLMNVKRGT YLPQTYIIQE

201 EMVVTEHVSD KEALGSFIYH LCNGKDTYRL RRRATRRRIN KRGAKNCNAI

251 RHFENTFVVE TLICGVV

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_16112, frame 1

SWISSNEW:ITMB_CHICK INTEGRAL MEMBRANE PROTEIN 2B (TRANSMEMBRANE PROTEIN E3-16)., N = 1, Score = 573, P = 1.4e-55

SWIΞSNEW:ITMB_MOUSE INTEGRAL MEMBRANE PROTEIN 2B (E25B PROTEIN)., N = 1, Score = 559, P = 4.2e-54

SWISSNEW:ITMA_HUMAN INTEGRAL MEMBRANE PROTEIN 2A (E25 PROTEIN)., N = 1, Score = 452, P = 9.1e-43

>SWISSNEW:ITMB_CHICK INTEGRAL MEMBRANE PROTEIN 2B (TRANSMEMBRANE PROTEIN E3-16) .

Length = ₂6₂

HSPs:

Score = 573 (86.0 bits), Expect = 1.4e-55, P = 1.4e-55 Identities = 118/264 (44%), Positives = 175/264 (66%)

Query: 1 MVKISFQPAVAGIKGDKADKASASAPAPASATEILLTPAREEQPPQHRSKRGGΞVGGVCY 60

MVK+SF A+A + A+K ++ ++L+ P + + P+ G C+ Sbjct: 1 MVKVSFNSALA--HKEAANKEEENS QVLILPP-DAKEPEDVVVPAGHKRAWCW 50

Query: 61 -LSMGMVVLLMGLVFASVYIYRYFFLAQLARDNFFRCGVLY-EDSLS SQVRTQM- 112

+ G+ +L G++ Y+Y+YF Q + CG+ Y ED LS +Q+++ Sbjct: 51 CMCFGLAFMLAGVILGGAYLYKYFAFQQ GGVYFCGIKYIEDGLSLPESGAQLKSARY 107

Query: 113 -ELEEDVKIYLDENYERINVPVPQFGGGDPADIIHDFQRGLTAYHDISLDKCYVIELNTT 171

+E++++I +E+ E I+VPVP+F DPADI+HDF R LTAY D+SLDKCYVI LNT+ Sbjct: 108 HTIEQNIQILEEEDVEFISVPVPEFADSDPADIVHDFHRRLTAYLDLSLDKCYVIPLNTS 167

Query: 172 IVLPPRNFWELLMNVKRGTYLPQTYIIQEEMVVTEHVSDKEALGSFIYHLCNGKDTYRLR 231

+V+PP+NF ELL+N+K GTYLPQ+Y+I E+M+VT+ + + + LG FIY LC GK+TY+L+ Sbjct: 168 VVMPPKNFLELLINIKAGTYLPQSYLIHEQMIVTDRIENVDQLGFFIYRLCRGKETYKLQ 227

Query: 232 RRATRRRINKRGAKNCNAIRHFENTFVVETLIC 264

R+ + I KR A NC IRHFEN F +ETLIC Sbjct: 228 RKEAMKGIQKREAVNCRKIRHFENRFAMETLIC 260

Pedant information for DKFZphfbr2_16112, frame 1

Report for DKFZphfbr2_16112.1

[LENGTH] 267

[MW] 30223.94 [pi] 8.16

[HOMOL] SWISSNEW:ITMB_CHICK INTEGRAL MEMBRANE PROTEIN 2B (TRANSMEMBRANE PROTEIN E3-16) le-49

[PROSITE] PRENYLATION 1

[PROSITE] MYRISTYL 5

[PROSITE] CAMP_PHOSPHO_SITE 2

[PROSITE] CK2_PHOSPHO_SITE 3

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 4

[PROSITE] ASN_GLYCOSYLATION 1

[KW] TRANSMEMBRANE 1

[KW] LOW COMPLEXITY 15.36 %

SEQ MVKISFQPAVAGIKGDKADKASASAPAPASATEILLTPAREEQPPQHRSKRGGSVGGVCY

SEG xxxxxxxxxxxxxxxx

PRD ccccccccchhhhhhhhhhhhhhhhhccccccceeecccccccccccccccccccccchh

MEM MMMMMMMMM

SEQ LSMGMVVLLMGLVFAΞVYIYRYFFLAQLARDNFFRCGVLYEDSLSSQVRTQMELEEDVKI

SEG .. xxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhcchhhhhhhhhhccceeeeeecccccccchhhhhhhhhhhhh

MEM MMMMMMMMMMMMMMMMMMM

SEQ YLDENYERINVPVPQFGGGDPADIIHDFQRGLTAYHDISLDKCYVIELNTTIVLPPRNFW SEG PRD hhcccceeeeccccccccccccchhhhhhhhhhhhhhhcccceeeeeccceeecccchhh MEM

SEQ ELLMNVKRGTYLPQTYIIQEEMVVTEHVSDKEALGSFIYHLCNGKDTYRLRRRATRRRIN SEG xxxxxxxxxxxx PRD hhhhhhcccccccceeeeehhhhhhhccccchhhhhheeeccccchhhhhhhhhhhhhhh MEM

SEQ KRGAKNCNAIRHFENTFVVETLICGVV SEG xx PRD hhhhccceeeecccchhhhhheeeccc MEM

Prosite for DKFZphfbr2_16112.1

PS00001 169->173 ASN_GLYCOSYLATION PDOC00001 PS00004 187->191 CAMP_PHOSPHO_SITE PDOC00004 PS00004 232->236 CAMP_PHOSPHO_SITE PDOC00004 PS00005 49->52 PKC_PHOSPHO_SITE PDOC00005 PS00005 209->212 PKC_PHOSPHO_SITE PDOC00005 PS00005 227->230 PKC_PHOSPHO_SITE PDOC00005 PS00005 235->238 PKC_PHOSPHO_SITE PDOC00005 PS00006 30->34 CK2_PHOSPHO_SITE PDOC00006 PS00006 110->114 CK2_PHOSPHO_SITE PDOC00006 PS00006 209->213 CK2_PHOSPHO_SITE PDOC00006 PS00007 119->127 TYR_PHOSPHO_SI E PDOC00007 PΞ00008 52->58 MYRISTYL PDOC00008 PS00008 53->59 MYRISTYL PDOC00008 PS00008 71->77 MYRISTYL PDOC00008 PS00008 138->144 MYRISTYL PDOC00008 PS00008 243->249 MYRISTYL PDOC00008 PS00294 264->268 PRENYLATION PDOC00266

(No Pfam data available for DKFZphfbr2_16112.1) DKFZphfbr2_22f21

group: brain derived

DKFZphfbr2_22f21 encodes a novel 567 amino acid protein with weak similarity to C. elegans cosmide C18C4.5

No informative BLAST results; no predictive prosite, pfam or SCOP motife

The new protein can find application in studying the expression profile of brain-specific genes . weak similarity to C. elegans C18C4.5

EST HSAA6531/HSAA5273/ defines splice variant, or unspliced cDNA additional -180 Bp at position 250

Sequenced by AGOWA

Locus: /map="311.4 cR from top of Chrl4 linkage group"

Insert length: 1910 bp

Poly A stretch at pos. 1887, polyadenylation signal at pos. 1867

1 TGGGCCCTTA GCAACGGCCT GGCGACGGTT TCCTTGCTGC TGCAGCCCCC

51 GTCGGCTCCT CTTTTCCAGT CCTCCACTGC CGGGGCTGGG CCCGGCCGCG

101 GGAAGGACCG AAGGGGATAC AGCGTGTCCC TGCGGCGGCT GCAAGAGGAC

151 TAAGCATGGA TGGCAGCCGG AGAGTCAGAG CAACCTCTGT CCTTCCCAGA

201 TATGGTCCAC CGTGCCTATT TAAAGGACAC TTGAGCACCA AAAGTAATGC

251 TGCAGTAGAC TGCTCGGTTC CAGTAAGCAT GAGTACCAGC ATAAAGTATG

301 CAGACCAACA ACGAAGAGAG AAACTCAAAA AGGAATTAGC ACAATGTGAA

351 AAAGAGTTCA AATTAACTAA AACTGCAATG CGAGCCAATT ATAAAAATAA

401 TTCCAAGTCA CTTTTTAATA CCTTACAAGA GCCCTCAGGC GAACCGCAAA

451 TTGAGGATGA CATGTTAAAA GAAGAAATGA ATGGATTTTC ATCCTTTGCA

501 AGGTCACTAG TACCCTCTTC AGAGAGACTA CACCTAAGTC TACATAAATC

551 CAGTAAAGTC ATCACAAATG GTCCTGAGAA GAACTCCAGT TCCTCCCCGT

601 CCAGTGTGGA TTATGCAGCC TCCGGGCCCC GGAAACTGAG CTCTGGAGCC

651 CTGTATGGCA GAAGGCCCAG AAGCACATTC CCAAATTCCC ACCGGTTTCA

701 GTTAGTCATT TCGAAAGCAC CCAGTGGGGA TCTTTTGGAT AAACATTCTG

751 AACTCTTTTC TAACAAACAA TTGCCATTCA CTCCTCGCAC TTTAAAAACA

801 GAAGCAAAAT CTTTCCTGTC ACAGTATCGC TATTATACAC CTGCCAAAAG

851 AAAAAAGGAT TTTACAGATC AACGGATAGA AGCTGAAACC CAGACTGAAT

901 TAAGCTTTAA ATCTGAGTTG GGGACAGCTG AGACTAAAAA CATGACAGAT

951 TCAGAAATGA ACATAAAGCA GGCATCTAAT TGTGTGACAT ATGATGCCAA

1001 AGAAAAAATA GCTCCTTTAC CTTTAGAAGG GCATGACTCA ACATGGGATG

1051 AGATTAAGGA TGATGCTCTT CAGCATTCCT CACCAAGGGC AATGTGTCAG

1101 TATTCCCTGA AGCCCCCTTC AACTCGTAAA ATCTACTCTG ATGAAGAAGA

1151 ACTGTTGTAT CTGAGTTTCA TTGAAGATGT AACAGATGAA ATTTTGAAAC

1201 TTGGTTTATT TTCAAACAGG TTTTTAGAAC GACTGTTCGA GCGACATATA

1251 AAACAAAATA AACATTTGGA GGGGGAAAAA ATGCGCCACC TGCTGCATGT

1301 CCTGAAAGTA GACTTAGGCT GCACATCGGA GGAAAACTCG GTAAAGCAAA

1351 ATGATGTTGA TATGTTGAAT GTATTTGATT TTGAAAAGGC TGGGAATTCA

1401 GAACCAAATA AATTAAAAAA TGAAAGTGAA GTAACAATTC AGCAGGAACG

1451 TCAACAATAC CAAAAGGCTT TGGATATGTT ATTGTCGGCA CCAAAGGATG

1501 AGAACGAGAT ATTCCCTTCA CCAACTGAAT TTTTCATGCC TATTTATAAA

1551 TCAAAGCATT CAGAAGGGGT TATAATTCAA CAGGTGAATG ATGAAACAAA

1601 TCTTGAAACT TCAACTTTGG ATGAAAATCA TCCAAGTATT TCAGACAGTT

1651 TAACAGATCG GGAAACTTCT GTGAATGTCA TTGAAGGTGA TAGTGACCCT

1701 GAAAAGGTTG AGATTTCAAA TGGATTATGT GGTCTTAACA CATCACCCTC

1751 CCAATCTGTT CAGTTCTCCA GTGTCAAAGG CGACAATAAT CATGACATGG

1801 AGTTATCAAC TCTTAAAATC ATGGAAATGA GCATTGAGGA CTGCCCTTTG

1851 GATGTTTAAT CTTCATTAAT AAATACCTCA AATGGCCAGT AAAAAAAAAA

1901 AAAAAAAAAA

BLAST Results

Entry HS477360 from database EMBL: human STS WI-14643.

Length = 418

Minus Strand HSPs:

Score = 1850 (277.6 bits), Expect = 2.5e-77, P = 2.5e-77

Identities = 392/405 (96%), Positives = 392/405 (96%), Strand = Minus /

Plus Medlme entries

No Medlme entry

Peptide information for frame 3

ORF from 156 bp to 1856 bp; peptide length: 567 Category: similarity to unknown protein

1 MDGSRRVRAT SVLPRYGPPC LFKGHLSTKΞ NAAVDCSVPV SMSTSIKYAD

51 QQRREKLKKE LAQCEKEFKL TKTAMRANYK NNSKSLFNTL QEPSGEPQIE

101 DDMLKEEMNG FSSFARSLVP SSERLHLSLH KSSKVITNGP EKNSSSSPSS

151 VDYAASGPRK LSSGALYGRR PRSTFPNSHR FQLVISKAPS GDLLDKHSEL

201 FSNKQLPFTP RTLKTEAKSF LSQYRYYTPA KRKKDFTDQR IEAETQTELS

251 FKSELGTAET KNMTDSEMNI KQASNCVTYD AKEKIAPLPL EGHDSTWDEI

301 KDDALQHSSP RAMCQYSLKP PSTRKIYSDE EELLYLSFIE DVTDEILKLG

351 LFSNRFLERL FERHIKQNKH LEGEKMRHLL HVLKVDLGCT SEENSVKQND

401 VDMLNVFDFE KAGNSEPNKL KNESEVTIQQ ERQQYQKALD MLLΞAPKDEN

451 EIFPSPTEFF MPIYKSKHSE GVIIQQVNDE TNLETSTLDE NHPSISDSLT

501 DRETSVNVIE GDSDPEKVEI SNGLCGLNTS PSQSVQFSSV KGDNNHDMEL 551 STLKIMEMSI EDCPLDV

BLASTP hits

Entry CEC18C4_3 from database TREMBL:

"C18C4.5"; Caenorhabditis elegans cosmid C18C4.

Length = 1091

Score = 98 (34.5 bits), Expect = 0.29, P = 0.25

Identities = 105/470 (22%), Positives = 192/470 (40%)

Alert BLASTP hits for DKFZphfbr2_22f21, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_22f21, frame 3

Report for DKFZphfbr2_22 21.3

[LENGTH] 567

[MW] 64120.02

[pi] 5.68

[PROSITE] AMIDATION 1

[PROSITE] MYRISTYL 3

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 16

[PROSITE] PKC_PHOSPHO_SITE 18

[PROSITE] ASN_GLYCOSYLATION 4

[KW] All_Alpha

[KW] LOW COMPLEXITY 1.23 %

SEQ MDGSRRVRATSVLPRYGPPCLFKGHLSTKSNAAVDCSVPVSMSTSIKYADQQRREKLKKE

SEG

PRD cccccceeeeeeccccccccccccccccccceeeecccccccchhhhhhhhhhhhhhhhh

SEQ LAQCEKEFKLTKTAMRANYKNNSKSLFNTLQEPSGEPQIEDDMLKEEMNGFSSFARSLVP

SEG

PRD hhhhhhhhhhhhhhhhhhhccccccceeecccccccchhhhhhhhhhhccccccceeecc

SEQ SSERLHLSLHKSSKVITNGPEKNSSSSPΞSVDYAASGPRKLSSGALYGRRPRSTFPNSHR

SEG xxxxxxx

PRD ccchhhhhhhhceeeecccccccccccccccccccccccccccccccccccccccccccc

SEQ FQLVISKAPSGDLLDKHSELFSNKQLPFTPRTLKTEAKSFLSQYRYYTPAKRKKDFTDQR

SEG

PRD cceeeeeccccccccccccccccccccccccchhhhhhhhhhhhhccccccchhhhhhhh

SEQ IEAETQTELSFKSELGTAETKNMTDSEMNIKQASNCVTYDAKEKIAPLPLEGHDSTWDEI

SEG

PRD hhhhhhhhhhhhhhccccccccccchhhhhhhccceeehhhhhhcccccccccccccccc SEQ KDDALQHSSPRAMCQYSLKPPSTRKIYSDEEELLYLSFIEDVTDEILKLGLFSNRFLERL SEG PRD cccccccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhccchhhhhhh

SEQ FERHIKQNKHLEGEKMRHLLHVLKVDLGCTSEENSVKQNDVDMLNVFDFEKAGNSEPNKL SEG PRD hhhhhhhhhhcccchhhhhhhhhccccccccccccccccccccceeeecccccccccccc

SEQ KNESEVTIQQERQQYQKALDMLLSAPKDENEIFPSPTEFFMPIYKSKHSEGVIIQQVNDE SEG PRD hhhhhhhhhhhhhhhhhhhhhhhcccccccccccccccccccccccccccceeeeecccc

SEQ TNLETSTLDENHPSISDSLTDRETSVNVIEGDSDPEKVEISNGLCGLNTSPSQSVQFSSV SEG PRD ccccccccccccccccccccccccceeecccccccceeeeccccccccccccceeeeecc

SEQ KGDNNHDMELSTLKIMEMSIEDCPLDV SEG PRD ccccccchhhhhhhhhhhhhccccccc

Prosite for DKFZphfbr2_22f21.3

PS00001 81->85 ASN_GLYCOSYLATION PDOC00001 PS00001 143->147 ASN_GLYCOSYLATION PDOC00001 PS00001 262->266 ASN_GLYCOSYLATION PDOC00001 PS00001 422->426 ASN_GLYCOSYLATION PDOC00001 PS00004 159->163 CAMP_PHOSPHO_SITE PDOC00004 PS00005 4->7 PKC_PHOSPHO_SITE PDOC00005 PS00005 27->30 PKC_PHOSPHO_SITE PDOC00005 PS00005 45->48 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 122->125 PKC_PHOSPHO_SITE PDOC00005 PS00005 132->135 PKC_PHOSPHO_SITE PDOC00005 PS00005 178->181 PKC_PHOSPHO_SITE PDOC00005 PS00005 202->205 PKC_PHOΞPHO_SITE PDOC00005 PS00005 209->212 PKC_PHOSPHO_SITE PDOC00005 PS00005 212->215 PKC_PHOSPHO_SITE PDOC00005 PS00005 250->253 PKC_PHOSPHO_SITE PDOC00005 PS00005 309->312 PKC_PHOSPHO_SITE PDOC00005 PS00005 317->320 PKC_PHOSPHO_SITE PDOC00005 PS00005 322->325 PKC_PHOSPHO_SI E PDOC00005 PS00005 353->356 PKC_PHOSPHO_SITE PDOC00005 PS00005 395->398 PKC_PHOSPHO_SITE PDOC00005 PS00005 500->503 PKC_PHOSPHO_SITE PDOC00005 PS00005 539->542 PKC_PHOSPHO_SITE PDOC00005 PS00005 552->555 PKC_PHOSPHO_SITE PDOC00005 PS00006 89->93 CK2_PHOSPHO_SITE PDOC00006 PS00006 149->153 CK2_PHOSPHO_SITE PDOC00006 PS00006 245->249 CK2_PHOSPHO_SITE PDOC00006 PS00006 264->268 CK2_PHOSPHO_SITE PDOC00006 PS00006 295->299 CK2_PHOSPHO_SITE PDOC00006 PS00006 328->332 CK2_PHOSPHO_SITE PDOC00006 PS00006 337->341 CK2_PHOSPHO_ΞITE PDOC00006 PΞ00006 390->394 CK2_PHOSPHO_SITE PDOC00006 PS00006 455->459 CK2_PHOSPHO_SITE PDOC00006 PS00006 481->485 CK2_PHOSPHO_SITE PDOC00006 PS00006 486->490 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 494->498 CK2_PHOSPHO_SITE PDOC00006 PS00006 498->502 CK2_PHOSPHO_SITE PDOC00006 PS00006 500->504 CK2_PHOSPHO_SITE PDOC00006 PS00006 513->517 CK2_PHOSPHO_SITE PDOC00006 PS00006 559->563 CK2_PHOSPHO_SITE PDOC00006 PS00008 164->170 MYRISTYL PDOC00008 PS00008 256->₂62 MYRISTYL PDOC00008 PS00008 350->356 MYRISTYL PDOC00008 PS00009 167->171 AMIDATION PDOC00009

(No Pfam data available for DKFZphfbr2_22f21.3) DKFZphfbr2_22hl3

group: transmembrane protein

DKFZphfbr2_22hl3 encodes a novel 520 ammo acid protein, with similarity to Drosophila melanogaster EG:39E1.3.

The protein contains an ATP/GTP A Prosite pattern (P-loop) . This loop interacts with one of the phosphate groups of a A or G nucleotide. It is found m numerous ATP- or GTP-binding proteins, such as ATP synthase alpha and beta subunits, Myosin heavy chains, Kinesin heavy chains and kinesin-like proteins, Dynamins and dynamin-like proteins, several kinases, DNA and RNA helicases, GTP-bind g elongation factors and the Ras family of GTP-bmding proteins. Additionally, the novel protein contains one putative transmembran domain.

No informative BLAST results; no predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes and as a new marker for neuronal cells.

AC004780_1, differences to predicted genmodel membrane regions: 1

AC004780_1, differences to predicted genmodel complete cDNA, complete eds, EST hits on genomic level encoded by AC004780, differences to predicted genmodel¹ TRANSMEMBRANE 1

Sequenced by AGOWA

Locus : unknown

Insert length: 2292 bp

Poly A stretch at pos. 2272, polyadenylation signal at pos. 2255

1 GGGGGAGGGA ACTGATCTCA GCTCGGGCCC GCGTTACATC CTCCTCCTCT 51 TCTTCCTTCG GCCCAGCTTT CCTTAGGGGC TGCAACCCGG ACGCCGAGGC

101 CGGTTTCGGA GTGGGGAGTG CCCATTTTCT CTCCTTCCCA CGTTCCTGGC

151 CCCCAGACGC CATTTGCAGG CGGGTGGCTT GGGTCAGCCT CCCCGCCCCC

201 ACCCGACTCC CGTCACGGGA GAGCGCACAC CGCGCCCCGA GAACCAATCA

251 GCAGCCGCGT TAGGTAACCA TGTCTGAGTC TGGACACAGT CAGCCTGGAC

301 TCTATGGGAT AGAGCGGCGG CGACGGTGGA AGGAGCCTGG CTCTGGTGGC

351 CCCCAGAATC TCTCTGGGCC TGGTGGTCGG GAGAGGGACT ACATTGCACC

401 ATGGGAAAGA GAGAGAAGGG ATGCCAGCGA AGAGACAAGC ACTTCCGTCA

451 TGCAGAAAAC CCCCATCATC CTCTCAAAAC CTCCAGCAGA GCGGTCAAAA

501 CAGCCACCAC CTCCAACAGC CCCTGCTGCC CCGCCTGCTC CAGCCCCTCT

551 GGAGAAGCCC ATCGTTCTCA TGAAGCCACG GGAGGAGGGG AAGGGGCCTG

601 TGGCCGTGAC AGGTGCCTCT ACCCCTGAGG GCACCGCCCC ACCACCCCCT

651 GCAGCCCCTG CGCCACCCAA GGGGGAGAAG GAGGGGCAGA GACCCACACA

701 GCCTGTGTAC CAGATCCAGA ACCGGGGCAT GGGCACTGCC GCACCAGCAG

751 CCATGGACCC TGTCGTGGGT CAGGCCAAAC TACTGCCCCC AGAGCGCATG

801 AAGCACAGCA TCAAGTTGGT GGATGACCAG ATGAATTGGT GTGACAGTGC

851 CATCGAGTAC CTGTTGGATC AGACTGATGT GTTGGTGGTT GGTGTCCTGG

901 GCCTCCAGGG GACAGGCAAG TCCATGGTCA TGTCATTGTT GTCAGCCAAC

951 ACTCCAGAGG AGGACCAGAG GACTTATGTT TTCCGGGCCC AGAGCGCTGA 1001 AATGAAGGAA CGAGGGGGCA ACCAGACCAG TGGCATCGAC TTCTTTATTA 1051 CCCAAGAACG GATTGTTTTC CTGGACACAC AGCCCATCCT GAGCCCTTCT 1101 ATCCTAGACC ATCTCATCAA TAATGACCGC AAACTGCCTC CAGAGTACAA 1151 CCTTCCCCAC ACTTACGTTG AAATGCAGTC ACTCCAGATT GCTGCCTTCC 1201 TTTTCACGGT CTGCCATGTG GTGATTGTTG TCCAGGACTG GTTCACAGAC 1251 CTCAGTCTCT ACAGGTTCCT GCAGACAGCA GAGATGGTGA AGCCCTCCAC 1301 CCCATCCCCC AGCCACGAGT CCAGCAGCTC ATCGGGCTCC GATGAAGGCA 1351 CCGAGTACTA CCCCCACCTA GTCTTCTTGC AGAACAAAGC TCGCCGAGAG 1401 GACTTCTGTC CTCGGAAGCT GCGGCAGATG CACCTGATGA TTGACCAGCT 1451 CATGGCCCAC TCCCACCTGC GTTACAAGGG AACTCTGTCC ATGTTACAAT 1501 GCAATGTCTT CCCGGGGCTT CCACCTGACT TCCTGGACTC TGAGGTCAAC 1551 TTATTCCTGG TACCCTTCAT GGACAGTGAA GCAGAGAGTG AAAACCCACC 1601 AAGAGCAGGA CCTGGTTCCA GCCCACTCTT CTCCCTGCTG CCTGGGTATC 1651 GTGGCCACCC CAGTTTCCAG TCCTTGGTGA GCAAGCTCCG GAGCCAAGTG 1701 ATGTCCATGG CCCGGCCACA GCTGTCACAC ACGATCCTCA CCGAGAAGAA 1751 CTGGTTCCAC TACGCTGCCC GGATCTGGGA TGGGGTGAGA AAGTCCTCTG 1801 CTCTGGCAGA GTACAGCCGC CTGCTGGCCT GAGGCCAAGG AGAGGAATGT 1851 CATGCAGGGG ACCTCCTGGG TCCGCAGTGT ACTGCGAGGG AGCACAGATG 1901 TCCATCCCCC GCTGGGGTGG AGAGCGGCAG CAGGCCTGAT GGATGAGGGA 1951 TCGTGGCTTC CCGGCCCAGA GACATGAGGT GTCCAGGGCC AGGCCCCCCA 2001 CCCTCAGTTG GGGCTGTTCC GGGGGTGACT GTGAGCGATC CCACCCCAAA

2051 CCTGAGATGG GGTAGCCCGT CCTGTGTCCT CCACAGGGAC AAGCAGTGGG

2101 AGGAGTCTGA ATGGTCACCA GGAAGCCCGG GCTCCATCTT GACCTCCTTT

2151 TTCAGGGACA GGAGCAACAG GCCCCTCTTC CCTGACTCTA AGCCCTTCCC

2201 TGTAAGGTGA GGCAGGGTCT GGAGAGCTCT TTATTGGAAC AGATCTGGTG

2251 GTTCAAATAA ACACAGTCAT GCAAAAAAAA AAAAAAAAAA AA

BLAST Results

Entry AC004780 from database EMBL:

Homo sapiens chromosome 19, cosmid F17127, complete sequence.

Score = 2616, P = O.Oe+00, identities = 524/525

15 exons Bp 8031-31789

Medlme entries

No Medlme entry

Peptide information for frame 3

ORF from 270 bp to 1829 bp; peptide length: 520 Category: similarity to unknown protein Prosite motifs: ATP GTP A (211-219)

1 MΞESGHSQPG LYGIERRRRW KEPGSGGPQN LSGPGGRERD YIAPWERERR

51 DASEETSTSV MQKTPIILSK PPAERSKQPP PPTAPAAPPA PAPLEKPIVL

101 MKPREEGKGP VAVTGASTPE GTAPPPPAAP APPKGEKEGQ RPTQPVYQIQ

151 NRGMGTAAPA AMDPVVGQAK LLPPERMKHS IKLVDDQMNW CDSAIEYLLD

201 QTDVLVVGVL GLQGTGKSMV MSLLSANTPE EDQRTYVFRA QSAEMKERGG

251 NQTSGIDFFI TQERIVFLDT QPILSPSILD HLINNDRKLP PEYNLPHTYV

301 EMQSLQIAAF LFTVCHVVIV VQDWFTDLSL YRFLQTAEMV KPSTPSPSHE

351 SSSSSGSDEG TEYYPHLVFL QNKARREDFC PRKLRQMHLM IDQLMAHSHL

401 RYKGTLSMLQ CNVFPGLPPD FLDSEVNLFL VPFMDSEAES ENPPRAGPGS

451 SPLFSLLPGY RGHPSFQSLV SKLRSQVMSM ARPQLSHTIL TEKNWFHYAA

501 RIWDGVRKSS ALAEYSRLLA

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_22hl3, frame 3

TREMBL:AC004780_1 product: "F17127_l"; Homo sapiens chromosome 19, cosmid F17127, complete sequence., N = 2, Score = 1264, P = 1.3e-231

TREMB :CEY54E2A_1 gene: "Y54E2A.2"; Caenorhabditis elegans cosmid Y54E2A, N = 2, Score = 219, P = 1.4e-15

>TREMBL:AC004780_1 product: "F17127_l"; Homo sapiens chromosome 19, cosmid F17127, complete sequence. Length = 528

HSPs:

Score = 1264 (189.6 bits), Expect = 1.3e-231, Sum P(2) = 1.3e-231 Identities = 254/302 (84%), Positives = 264/302 (87%)

Query: 46 ERERRDASEETSTSVMQKTPIILSKPPAERSKQPPPPTAPAAPPAPAPLEKPIVLMKPRE 105

E+ER D+ + S +Q+T + R + P + A APLEKPIVLMKPRE Sbjct: 39 EKER-DSDSDFΞP—LQQTEGCQRRDKHFRHAENPHHPLKTSSRA-APLEKPIVLMKPRE 94

Query: 106 EGKGPVAVTGASTPEGTAPPPPAAPAPPKGEKEGQRPTQPVYQIQNRGMGTAAPAAMDPV 165

EGKGPVAVTGASTPEGTAPPPPAAPAPPKGEKEGQRPTQPVYQIQNRGMGTAAPAAMDPV Sbjct: 95 EGKGPVAVTGASTPEGTAPPPPAAPAPPKGEKEGQRPTQPVYQIQNRGMGTAAPAAMDPV 154

Query: 166 VGQAKLLPPERMKHSIKLVDDQMNWCDSAIEYLLDQTDVLVVGVLGLQGTGKSMVMSLLS 225

VGQAKLLPPERMKHSIKLVDDQMNWCDSAIEYLLDQTDVLVVGVLGLQGTGKSMVMSLLS Sbjct: 155 VGQAKLLPPERMKHSIKLVDDQMNWCDSAIEYLLDQTDVLVVGVLGLQGTGKSMVMSLLS 214 Query: 226 ANTPEEDQRTYVFRAQSAEMKERGGNQTSGIDFFITQERIVFLDTQPILΞPSILDHLINN 285 ANTPEEDQRTYVFRAQSAEMKERGGNQTSGIDFFITQERIVFLDTQPILSPSILDHLINN

Sbjct: 215 ANTPEEDQRTYVFRAQSAEMKERGGNQTSGIDFFITQERIVFLDTQPILSPSILDHLINN 274

Query: 286 DRKLPPEYNLPHTYVEMQSLQIAAFLFTVCHVVIVVQDWFTDLSLYRFLQTAEMVKPSTP 345 DRKLPPEYNLPHTYVEMQSLQIAAFLFTVCHVVIVVQDWFTDLSLYR K ++

Sbjct: 275 DRKLPPEYNLPHTYVEMQSLQIAAFLFTVCHVVIVVQDWFTDLSLYRLWDLGCKCKΞNSH 334

Query: 346 SP 347

SP

Sbjct: 335 SP 336

Score = 993 (149.0 bits), Expect = 1.3e-231, Sum P(2) = 1.3e-231 Identities == 189/189 (100%), Positives = 189/189 (100%)

Query: 332 RFLQTAEMVKPSTPSPSHESSSSSGSDEGTEYYPHLVFLQNKARREDFCPRKLRQMHLMI 391 RFLQTAEMVKPSTPSPSHESSSSSGSDEGTEYYPHLVFLQNKARREDFCPRKLRQMHLMI Sbjct: 340 RFLQTAEMVKPΞTPSPSHESSSSSGSDEGTEYYPHLVFLQNKARREDFCPRKLRQMHLMI 399 Query: 392 DQLMAHSHLRYKGTLSMLQCNVFPGLPPDFLDSEVNLFLVPFMDSEAESENPPRAGPGSS 451 DQLMAHSHLRYKGTLSMLQCNVFPGLPPDFLDΞEVNLFLVPFMDSEAESENPPRAGPGSS Sbjct: 400 DQLMAHSHLRYKGTLSMLQCNVFPGLPPDFLDSEVNLFLVPFMDSEAESENPPRAGPGSS 459 Query: 452 PLFSLLPGYRGHPSFQSLVSKLRSQVMSMARPQLSHTILTEKNWFHYAARIWDGVRKSΞA 511 PLFSLLPGYRGHPSFQSLVSKLRSQVMSMARPQLSHTILTEKNWFHYAARIWDGVRKSSA Sbjct: 460 PLFSLLPGYRGHPSFQSLVSKLRSQVMSMARPQLSHTILTEKNWFHYAARIWDGVRKSSA 519 Query: 512 LAEYSRLLA 520 LAEYSRLLA Sbjct: 520 LAEYSRLLA 528

Pedant information for DKFZphfbr2_22hl3, frame 3

Report for DKFZphfbr2_22hl3.3

[LENGTH] 520

[MW] 57650.81

[pi] 6.52

[HOMOL] TREMBL:AC004780_1 product: "F17127_l"; Homo sapiens chromosome 19, cosmid

F17127, complete sequence. 0.0

[PROSITE] A P_GTP_A 1

[PROSITE] MYRISTYL 8

[PROSITE] CAMP_PHOSPHO_SITE

[PROSITE] CK2_PHOSPHO_SITE

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE 3

[PROSITE] ASN_GLYCOSYLATION 2

[KW] TRANSMEMBRANE 1

[KW] LOW COMPLEXITY 11.73 %

SEQ MSESGHSQPGLYGIERRRRWKEPGSGGPQNLSGPGGRERDYIAPWERERRDASEETSTSV

SEG

PRD cccccccccccccccccccccccccccccccccccccceeeeehhhhhhhhhccccccee

MEM

SEQ MQKTPIILSKPPAERSKQPPPPTAPAAPPAPAPLEKPIVLMKPREEGKGPVAVTGASTPE

SEG xxxxxxxxxxxxxxx

PRD eeccceeecccccccccccccccccccccccccccceeeeeccccccccceeeecccccc

MEM

SEQ GTAPPPPAAPAPPKGEKEGQRPTQPVYQIQNRGMGTAAPAAMDPVVGQAKLLPPERMKHS

SEG .. xxxxxxxxxxx

PRD cccccccccccccccccccccccceeeeeeccccccccccccceeecceeecccchhhhh

MEM

SEQ IKLVDDQMNWCDSAIEYLLDQTDVLVVGVLGLQGTGKSMVMSLLSANTPEEDQRTYVFRA

SEG xxxxxxxxxxxxxxxxxxx

PRD hhhhcccchhhhhhhhhhccccceeeeeecccccccchhhhhhhhccccchhhhhheeee

MEM

SEQ QSAEMKERGGNQTSGIDFFITQERIVFLDTQPILSPSILDHLINNDRKLPPEYNLPHTYV

SEG

PRD hhhhhhhcccccceeeeeeeecceeeeeeccccccccccccccccccccccccccccchh

MEM

SEQ EMQSLQIAAFLFTVCHVVIVVQDWFTDLSLYRFLQTAEMVKPSTPSPSHESSSSSGSDEG

SEG xxxxxxxxxxxxxxxx ... PRD hhhhhhhhhhhhhhhheeeeeeeccchhhhhhhhhhhhhhhccccccccccccccccccc MEM MMMMMMMMMMMMMMMMMMMMMMM

SEQ TEYYPHLVFLQNKARREDFCPRKLRQMHLMIDQLMAHSHLRYKGTLSMLQCNVFPGLPPD SEG PRD cccccceeeehhhhhhhcccccchhhhhhhhhhhhhhhhhhccccccccccccccccccc MEM

SEQ FLDSEVNLFLVPFMDSEAESENPPRAGPGΞSPLFSLLPGYRGHPSFQSLVSKLRSQVMSM SEG PRD chhhhhheeeeeccccccccccccccccccccceeeccccccccchhhhhhhhhhhhhhh

MEM

SEQ ARPQLSHTILTEKNWFHYAARIWDGVRKSSALAEYSRLLA SEG PRD hhhhhhhheeeccchhhhhhhhhhhhcchhhhhhhhhccc MEM

Prosite for DKFZphfbr2_22hl3.3

PS00001 30->34 ASN_GLYCOSYLATION PDOC00001 PS00001 251->255 ASN_GLYCOΞYLATION PDOC00001 PS00002 32->36 GLYCOSAMINOGLYCAN PDOC00002 PS00004 507->511 CAMP_PHOSPHO_SITE PDOC00004 PS00005 180->183 PKC_PHOSPHO_SITE PDOC00005 PS00005 215->218 PKC_PHOSPHO_SITE PDOC00005 PS00005 491->494 PKC_PHOSPHO_SITE PDOC00005 PS00006 117->121 CK2_PHOΞPHO_SITE PDOC00006 PS00006 193->197 CK2_PHOSPHO_SITE PDOC00006 PS00006 ₂₂8->232 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 254->258 CK2_PHOSPHO_SITE PDOC00006 PS00006 277->281 CK2_PHOSPHO_SITE PDOC00006 PS00006 298->302 CK2_PHOSPHO_SITE PDOC00006 PS00006 355->359 CK2_PHOSPHO_SITE PDOC00006 PS00006 436->440 CK2_PHOSPHO_SITE PDOC00006 PS00008 26->32 MYRISTYL PDOC00008 PS00008 139->145 MYRISTYL PDOC00008 PΞ00008 153->159 MYRISTYL PDOC00008 PS00008 211->217 MYRISTYL PDOC00008 PS00008 214->220 MYRISTYL PDOC00008 PS00008 249->255 MYRISTYL PDOC00008 PS00008 356->362 MYRISTYL PDOC00008 PS00008 505->511 MYRISTYL PDOC00008 PS000Ϊ7 211->219 ATP GTP A PDOC00017

(No Pfam data available for DKFZphfbr2 22hl3.3)

DKFZphfbr2_22ι4

group: brain derived

DKFZphfbr2_22ι4.1 encodes a novel 228 amino acid protein with similarity to the N-termmus of human p52rIPK.

No informative BLAST results; no predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . similarity to Human P52rIPK N-terminus complete cDNA, complete eds, few EST hits function of P52rIPK, repressor of p58IPK protein kinase inhibitor upstream regulator of interferon induced proteins

Sequenced by AGOWA

Locus: unknown

Insert length: 4748 bp

Poly A stretch at pos. 4726, polyadenylation signal at pos. 4709

1 TGGGTCCGGT CCTAGGGTCA CACCCACCGC AGGGTCTGGC TTGGTACAGT

51 TGGGTGCATG CAGAAGTAGG TGGAGCTGCT GTTGCAGCCT TGAGAGAGTT

101 TTATTGTAAA ACTCTTGTAA TTTATAGTAA TCGGAGGGGA AAACACCTCT

151 TCCTTTTAAT TGCTCTGAGG ACCGCTGCCA AAGAAACGCA GTAGATCCGC

201 TCCCTCTTGG GGGCGGGGAG AAAGAACGGG TTGTGTCCGC CATGTTGGTG

251 AAGTCAAGCG AAGGCGACTA GAGCTCCAGG AGGGCCAGTT CTGTGGGCTC

301 TAGTCGGCCA TATTAATAAA GAGAAAGGGA AGGCTGACCG TCCTTCGCCT

351 CCGCCCCCAC ATACACACCC CTTCTTCCCA CTCCGCTCTC ACGACTAAGC

401 TCTCACGATT AAGGCACGCC TGCCTCGATT GTCCAGCCTC TGCCAGAAGA

451 AAGCTTAGCA GCCAGCGCCT CAGTAGAGAC CTAAGGGCGC TGAATGAGTG

501 GGAAAGGGAA ATGCCGACCA ATTGCGCTGC GGCGGGCTGT GCCACTACCT

551 ACAACAAGCA CATTAACATC AGCTTCCACA GGTTTCCTTT GGATCCTAAA

601 AGAAGAAAAG AATGGGTTCG CCTGGTTAGG CGCAAAAATT TTGTGCCAGG

651 AAAACACACT TTTCTTTGTT CAAAGCACTT TGAAGCCTCC TGTTTTGACC

701 TAACAGGACA AACTCGACGA CTTAAAATGG ATGCTGTTCC AACCATTTTT

751 GATTTTTGTA CCCATATAAA GTCTATGAAA CTCAAGTCAA GGAATCTTTT

801 GAAGAAAAAC AACAGTTGTT CTCCAGCTGG ACCATCTAAT TTAAAATCAA

851 ACATTAGTAG TCAGCAAGTA CTACTTGAAC ACAGCTATGC CTTTAGGAAT

901 CCTATGGAGG CAAAAAAGAG GATCATTAAA CTGGAAAAAG AAATAGCAAG

951 CTTAAGAAGA AAAATGAAAA CTTGCCTACA AAAGGAACGC AGAGCAACTC

1001 GAAGATGGAT CAAAGCCACG TGTTTGGTAA AGAATTTAGA AGCAAATAGT

1051 GTATTACCTA AAGGTACATC AGAACACATG TTACCAACTG CCTTAAGCAG

1101 TCTTCCCTTG GAAGATTTTA AGATCCTTGA ACAAGATCAA CAAGATAAAA

1151 CACTGCTAAG TCTAAATCTA AAACAGACCA AGAGTACCTT CATTTAAATT

1201 TAGCTTGCAC AGAGCTTGAT GCCTATCCTT CATTCTTTTC AGAAGTAAAG

1251 ATAATTATGG CACTTATGCC AAAATTCATT ATTTAATAAA GTTTTACTTG

1301 AAGTAACATT ACTGAATTTG TGAAGACTTG ATTACAAAAG AATAAAAAAC

1351 TTCATATGGA AATTTTATTT GAAAATGAGT GGAAGTGCCT TACATTAGAA

1401 TTACGGACTT AAAAATTTTG CTAATAAATT GTGTGTTTGA AAGGTGTTTT

1451 TTGTTTTTGT CTTTTTAAAC TACTGTTAAA AGAACAGCTT ATGATAAGTA

1501 ATATGTTTAA CTTAGAGAAG AATTTTTTCC TGTACCAAAG TTGGCATATT

1551 GCATTCTAAA TAAGATGCTA AATAAGAGTT AACCAACATT CAACATGACC

1601 TTAAAACTGC TGGGTTTTGT ATTAATTAAA TTATAATTGG CACTGTGATT

1651 TGAAAAATTT ATAGAAAAAA AGGTACAGGG CAAGTTTTTA AATTAAAACT

1701 TTCTATATTT TGTTTTACCA GTAAAAGTGA GCTTATCATG GCCTCTCTCA

1751 TAAGAATGAT TTTAAAATAG GTTGTAAAAT ATTTTGAAAA TATTTGAATG

1801 TGAAGTACCA TTGAGTCATC CAAACTAGGT AAGGCCTCAA GTACTTTAAA

1851 CTAGTAAAAT CTAGTAGCTG ATAATATTCA CCTAAGTAAG TGTTGTAAAA

1901 TAATTCAGAG TTCAGGACCT AGCTTAGATA AATGTATACT ACTCTTTTTC

1951 TCATAGTAAA AATCTTACAT TTCCAACTTC AAAATTGGTG CTTCCATATT

2001 TGTTGATAAC CAAAACTCCT AAGGTTTTTT GTTTTCTTTT TAACTACTTT

2051 CCAAATGCAT ACTATACCTC AGAAATAGTG TATCAATATA GTGGGCTTTT

2101 TTTTTCCTCT TCATAAACCC ACAGTAAAAT TTAATCACAG GAAACTACTT

2151 ATATCTTCAC ACTTTGTATT GATAACTTAA AATGGCATCA GTTTATCTTA

2201 GACATCAGCT TGCTTTTTAT CTCCTTTTTT AGTGAGTGAA ATAGAGCAAC

2251 TAGCATGCCT GTGTTCCCAG CTACTTGGGA GGCTAAGGTG GGAAGATCAA

2301 TTGAACCTAG GAGGTTGAGG CTATAGTGAG CTGTGATTGC ACGACTGCAC

2351 TCCAGCCTGG GCAATGGAGT GAGACTCCTG TCTCTAAAAC AGCAACAACA

2401 AAAATAAAGC AACCATAGTG CATAAGGGAA ATTAAATGTT CCCTATAGAA

2451 ATATGTGTAT GTCTGTGATA GTGGTATGCA AATGCTAATT ATTTTATAAA

2501 ATAAAAGTTC AGAACTATTC TTATCATTGC CACTTGAACA ATTAAAGGGT

2551 TTGCTTTATT TCACTAATGT TTAATAGGAA CCCTTTGCTT CAAACAGCTT 2601 TGTTGAAATC ATGTAAAAAT TTGTTAATAG AGAATCAAGT TATTTAACTC

2651 AACTTATTTA ATTCAAGCTT GTGATACTAA CATACAAAGG TAGCATAAAC

2701 CAAGTCATAA ATTGCTGTAA TCTTTCCTGT AGAGTAATAG CTACTTCATG

2751 ATTTTTTTAA AAATTTCATT TTTTTGCTAT TTAGGATTGC ATTTGCTTGG

2801 CTCCTAGTAA CAATTCTTTT ACAGTATTAG CACTCTCTTT ACTAAGGAAT

2851 GCCTCCCAAG GAAATGCAAA GGTAGGAAAA GTCTCTTAGA ATGCCCATGA

2901 GGTATTTAAA ACAGATATTT ATGAAAATCT TTTTGTGAAT GTTATAAATC

2951 TTGCTAGTTA TTTTATCTTT ATCTTAAGTA TTAGATGTAG TTCCTTGGAA

3001 TTGTCATTAC ATATTTATTT TTTTCTAGTG TGGTTTCAAA TAACTTTTTG

3051 CCAACATATA ATCATCATCA AACATTCACT GACCATATCT ATTTTATAAC

3101 TCAAAATAAG TTGGACAAAT AATCATTTTA ATAAAAACTA TTTTTTCCAA

3151 GTATAACCAC TGTCATGTGG TTCACCCTTC ACCCCAGATA CAAAACACTT

3201 ATTTGTGTAG CCCAGTTCCC ATCTACAGTA ATACCTTGAA ACCTTAATAA

3251 ATTTTAAAAA TCATAAAAAT AAAATATTGT AAAATACAAC AAATTTTGGA

3301 CAAGGTTACT TCATCTTCAT TCATTATTAC CTGACAGTAT TAAACTACTA

3351 CTCAATAATT TTAGAGTAAA CTTTTCTGTG TTTTCCCCGT GATTTTCATT

3401 GTGCTGTCCT GACAACATGC TCCAAACTCT TTGCATCAAA TTGTTTTATT

3451 AACATACATT TGTCTACCTT AAAACTAGCT TTATTCACAG AGAAAGACCT

3501 AAAAGGAGTC TATTAAAATG CTGCTTTCAG TTTGATAGTT TTTTTTTTAA

3551 TCACTCTGAC CATAAACTAA CTGAAATTAT AATGGATTTT TTTTCCTCTC

3601 CCGGTCACAA CACAGATCTT CTGTTCATTT GTTCTCTGTC TACTGGGCAC

3651 CAACCTCTAC AAAGAACCAG CCAAAGGCTA GGTACTTGAT ATAAAAAGGA

3701 ATATTACATT ATTTTCTGCC CTCAAGTTGC TCTATCTCCT GAAAGAAACA

3751 AGTAATATTT ATAATACAAT ATGATAAATG CTACAAAAGA AATAGCTGTA

3801 AAGTCCTTTG GTAAATGCTG TTGAATTGGA ATTCAGTAAG AACTATAAAC

3851 TGTAGACCTT TTTATAATCA AATGCTTTTG TCTTGAAACA AAACAGATTC

3901 CTCCTTATAT TGACTTAGCA AAGGAGGTAC AAGGACATTG GCATTTGACC

3951 TGAATTATGG TGTTTTATTG AATGAGCTAT AAGACAACAT TTTTACCCTT

4001 TAAAATGAAC ACTGAACAAA TGTGTTAATG GTATCTTTGT TAAAAGGAAA

4051 ACATAGCTAT AAATAAAATA CTACATCGAA ATCCAGCACT GGAGTTCATT

4101 TGAAATTTGA TATTTTGTGT AAAGTAACAA ACCTATTAAC ACAGATTTTT

4151 AAAATAACTC AGAATCGTAT AAAGCACTTT GGTACTTATT TGTTCTCTTT

4201 TCCCTTACAT TCTGTGTGGT AGGTGGTATT ATCTCTGATT TACACATGAA

4251 GACATCCTTG TTAATGCAAT TTATTTATTC ATTCGGGCAT TTACTGTGTG

4301 CCAACTTGCA AAAGGAATAG AAATGTCTGT GATCTAGATA GTTCTAGATT

4351 GAACATAGAT TTTCTGCCAA CAAATCCTCT CTGCTGTTCA CATTATCCTT

4401 TGTTTAACGT ATGAACCAGG TTACTAAAAT AGGATAAATC ATGTGTCTTA

4451 GAATATGAAA ATAGTAAGGT CTTTGAGGTC ACTTGATCTT CTCTAAGTAG

4501 ACTTTATAAT ATTGTGTTTT ATCTCATTTC TCAATATTAG AATACGGGTA

4551 GATTTTAATT TTGCTATAAT ATAGGAAATG GTTCATCTTT GTACCAAAAT

4601 ATTGCATTCT TCTGATATTT AGACAGTTGG AAACTTTCTA AAATTGAGGA

4651 TTTTGTAGTG TATACTAAAT AATTGCATAT TCAAAAAAAT GTATTCTGAG

4701 TATGGTGATA TTAAACATTT TTCCCCAAAA AAAAAAAAAA AAAAAAAA

BLAST Results

No BLAST result

Medline entries

98107671:

Regulation of mterferon-induced protein kinase PKR: modulation of P58IPK inhibitory function by a novel protein,

P52rIPK

Peptide information for frame 1

ORF from 511 bp to 1194 bp; peptide length: 228 Category: similarity to known protein

1 MPTNCAAAGC ATTYNKHINI ΞFHRFPLDPK RRKEWVRLVR RKNFVPGKHT

51 FLCΞKHFEAS CFDLTGQTRR LKMDAVPTIF DFCTHIKSMK LKSRNLLKKN

101 NSCSPAGPSN LKSNISSQQV LLEHSYAFRN PMEAKKRIIK LEKEIASLRR

151 KMKTCLQKER RATRRWIKAT CLVKNLEANS VLPKGTSEHM LPTALSSLPL

201 EDFKILEQDQ QDKTLLSLNL KQTKSTFI

BLASTP hits

Entry AF007393_1 from database TREMBL: product: "P52rIPK"; Homo sapiens P52rIPK mRNA, complete eds.

Score = 166, P = 2.5e-ll, identities = 40/106, positives = 56/106 Alert BLASTP hits for DKFZphfbr2_22ι4, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_22ι4, frame 1

Report for DKFZphfbr2_22ι4.1

[LENGTH] 228

[MW] 26259.94

[pi] 10.17

[HOMOL] TREMBL:AF007393_1 product: 'P52rIPK"; Homo sapiens P52rIPK mRNA, complete eds. le-09

[PROSITE] MYRISTYL 1

[PROSITE] CAMP_PHOΞPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 2

[PROSITE] PKC_PHOSPHO_SITE 4

[PROSITE] ASN_GLYCOSYLATION 3

[KW] All_Alpha

[KW] LOW COMPLEXITY 7.02 %

SEQ MPTNCAAAGCATTYNKHINISFHRFPLDPKRRKEWVRLVRRKNFVPGKHTFLCSKHFEAS SEG PRD cccccccccccccccccccceeeecccccchhhhhhhhhhhhhcccccceeehhhhhhhh

SEQ CFDLTGQTRRLKMDAVPTIFDFCTHIKSMKLKSRNLLKKNNSCSPAGPSNLKSNISSQQV SEG xxxxxxxxxxxxxxxx PRD cccccccccccccccccceeeeccccchhhhhhhhhhhccccccccccccccccccchhh

SEQ LLEHSYAFRNPMEAKKRIIKLEKEIASLRRKMKTCLQKERRATRRWIKATCLVKNLEANS SEG PRD hhhhcccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhheeeecccccc

SEQ VLPKGTSEHMLPTALSSLPLEDFKILEQDQQDKTLLSLNLKQTKSTFI SEG PRD cccccccccccccccccccccchhhhhhcccccccccccccccccccc

Prosite for DKFZphfbr2_22ι4.1

PS00001 19->23 ASN GLYCOSYLATION PDOC00001

PS00001 100->104 ASN GLYCOSYLATION PDOC00001

PS00001 114-M18 ASN GLYCOSYLATION PDOC00001

PS00004 160->164 CAMP PHOSPHO SITE PDOC00004

PS00005 68->71 PKC PHOSPHO SITE PDOC00005

PS00005 88->91 PKC PHOSPHO SITE PDOC00005

PS00005 147->150 PKC PHOSPHO SITE PDOC00005

PΞ00005 163->166 PKC PHOSPHO SITE PDOC00005

PS00006 60->64 CK2 PHOSPHO SITE PDOC00006

PS00006 78->82 CK2 PHOSPHO SITE PDOC00006

PS00008 9->15 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2 22ι4.1)

DKFZphfbr2_22k3

group: brain derived

DKFZphfbr2_22k3 encodes a novel 538 amino acid protein with weak similarity to extensins.

No informative BLAST results; no predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . weak similarity to extensins complete cDNA, complete eds, few EST hits CpG Island in 5' UTR complete cDNA

Sequenced by AGOWA

Locus : unknown

Insert length: 2775 bp

Poly A stretch at pos. 2755, polyadenylation signal at pos. 2718

1 GGGGCTGCCC GCGCGCTCCA CGGTGCAGAG CTCTAAGCGC GCGGGCTGGC

51 AGGCTGCGGC GCGTCAAGGT CAGCCTGGAG CTGGGTGGCG GCCTGCCTGG

101 GGGCGGGGGA CCCTACTGGA GGCCCGGGCT GGGGCCTCCC AGCGCCTCGG

151 CCATATTGAA TAGCTTCGAC TGGACCGTCT TTGTCTGCGA AGTCCTGTCC

201 CAAGTTCCAG CCGCGTCCCT GGGGCCTGGG GCAGGAAGAG TCGCTGGCAG

251 CCCGCGCGCC CCAACTTGGA GCTGGGACAC CACGTTTCCA GCTTGGAGTG

301 GGCCTTGAGC CTTGGGACTG ACCTCGCCCC CGGCTCACGT AGGCATCCTG

351 GAAATTGATT CCCCCAAGTC CTTGGTGGGG GAGCCGGACT TGGTCAAGAC

401 TGTACTTGTT GCAGGCGAAG AGATTGGAGG CGTTTGGCTC GTCCCTGGCT

451 AGGGAGGTGA GACTCTCCGG TCAGCGTTGC TGGAACTCCC CCCATCCAGT

501 CCCTCCCTCA AGACTAAGGG CTACAGTAGT TTGTTGGGGC TCATTGCCCC

551 CTCACCCCAG ATATCACCCT GGAGATCTTA AAGACTCTCG AGAAAAGCCA

601 CGTGGGGGGC TGGTTCCCCT GGGGCTTCCT GCCGTCCCCC GACTGCCTCA

651 TTCTTTGGAG CGTCCCCGAT GTCTGCAAAG ATGTGGATTT GGACGTCCTC

701 GTGGAAGCCC TAAAGCCCGT GGGGACATTT AAGAAGATCG GCAAGGTGTT

751 CCGCAAGGAG GAGGACTCCA CGGTGGGGAT GCTGCAGATC GGGGAGGACG

801 TCGACTATTT GCTCATCCCC CGGGAGGTCA GGCTGGCTGG GGGCGTCTGG

851 AGAGTCATCT CTAAGCCCGC CACCAAGGAA GCAGAATTTC GGGAGCGGCT

901 GACCCAGTTC CTGGAAGAAG AGGGCCGCAC CCTGGAGGAC GTGGCCCGCA

951 TCATGGAGAA GAGCACCCCG CACCCGCCCC AGCCCCCCAA AAAGCCCAAG

1001 GAGCCCCGAG TGAGGAGGAG AGTGCAGCAG ATGGTGACTC CTCCGCCCCG

1051 GCTGGTCGTG GGCACGTACG ACAGCAGCAA CGCCAGCGAC AGCGAGTTCA

1101 GCGACTTCGA GACCTCCAGA GACAAGAGCC GCCAGGGCCC GCGGCGGGGC

1151 AAGAAGGTGC GCAAAATGCC CGTCAGCTAC CTGGGCAGCA AGTTCCTGGG

1201 AAGCGACCTG GAGAGTGAGG ATGATGAGGA ACTGGTCGAG GCCTTCCTCC

1251 GGCGACAGGA GAAGCAGCCC AGCGCGCCGC CTGCCCGCCG CCGCGTCAAC

1301 CTGCCAGTGC CCATGTTTGA GGACAACCTG GGGCCTCAGC TGTCCAAAGC

1351 GGACAGGTGG CGGGAGTATG TCAGCCAGGT GTCCTGGGGG AAGCTGAAGC

1401 GGAGGGTGAA GGGTTGGGCG CCGAGGGCGG GCCCCGGGGT GGGCGAGGCC

1451 CGGCTGGCCT CCACCGCAGT GGAGAGCGCA GGGGTATCAT CGGCGCCAGA

1501 GGGCACCAGC CCGGGGGATC GCTTGGGAAA CGCGGGAGAT GTTTGTGTGC

1551 CCCAGGCTTC CCCTAGGCGA TGGAGGCCCA AGATCAACTG GGCCTCCTTT

1601 CGGCGCCGCA GGAAGGAGCA GACAGCACCC ACAGGTCAGG GGGCAGACAT

1651 CGAGGCTGAT CAGGGGGGAG AGGCTGCAGA TAGTCAAAGG GAAGAGGCCA

1701 TAGCTGACCA GCGGGAAGGG GCTGCAGGTA ATCAGAGGGC TGGGGCCCCA

1751 GCTGACCAGG GGGCAGAGGC TGCAGATAAT CAGAGGGAAG AGGCTGCAGA

1801 TAATCAGAGG GCAGGGGCCC CAGCTGAGGA GGGGGCAGAG GCTGCAGATA

1851 ACCAGAGGGA AGAGGCTGCA GATAATCAGA GGGCAGAGGC CCCAGCTGAC

1901 CAGAGGTCAC AGGGCACAGA TAACCACAGG GAAGAGGCTG CAGATAATCA

1951 GAGGGCGGAG GCCCCAGCTG ACCAGGGGTC AGAGGTTACA GATAATCAAA

2001 GGGAAGAGGC CGTACATGAC CAGAGGGAAA GGGCCCCAGC TGTCCAGGGT

2051 GCAGATAATC AGAGGGCACA GGCCCGGGCT GGCCAGAGGG CAGAGGCTGC

2101 ACATAATCAG AGGGCAGGGG CCCCAGGTAT CCAGGAAGCT GAAGTCTCAG

2151 CTGCCCAAGG GACCACAGGA ACAGCTCCAG GAGCCAGGGC CCGGAAACAG

2201 GTCAAGACAG TGAGGTTCCA GACCCCTGGA CGCTTTTCGT GGTTTTGCAA

2251 GCGCCGGAGA GCCTTCTGGC ACACTCCCCG GTTGCCAACC CTGCCCAAGA

2301 GAGTCCCCAG GGCAGGAGAG GTCAGGAACC TCAGGGTGCT GAGGGCCGAG

2351 GCCAGAGCAG AAGCTGAGCA GGGAGAGCAA GAAGACCAGC TGTGAGGTGA

2401 GGGCTAGAGA CAGCCCACGG GCCCTCCCTC CAAGTGTGGG AGGGAGAGAT

2451 GCTCTGCCTC TGAACTTCAA AGTGGAGGTG GAGTGCTGGC CACGTCTCCA

2501 CCTAACAACC CTCTTTATTC TCTTGTTAAA GTTTTGTTCA TGCTTTGATT

2551 TTTTTTTAAA TTTTTTAGAG ACAGGGTCTC ACTCTGTTGC CCAGGCTGGA

2601 GTGCAGTGGC ATGATCATAA CTCACTGCAG CCTCAAACTT CTGGCCTCAA

2651 GTGATCCTCC TGCCTCGGCC TCCCAAAATG CTGGGATTAC AGATGTGAGC 2701 CACCACACAC ACCATCTGAT TAAAAAAAAA AAATACTGAT TCCCTGTAGC 2751 AACCCAAAAA AAAAAAAAAA AAAAA

BLAST Results

Entry HS164A7F from database EMBL:

H. sapiens CpG island DNA genomic Msel fragment, clone 164a7, forward read cpgl64a7. ftla . Score = 740, P = 3.0e-25, identities = 150/151

Medlme entries

No Medlme entry

Peptide information for frame 2

ORF from 779 bp to 2392 bp; peptide length: 53£ Category: similarity to known protein

1 MLQIGEDVDY LLIPREVRLA GGVWRVISKP ATKEAEFRER LTQFLEEEGR 51 TLEDVARIME KSTPHPPQPP KKPKEPRVRR RVQQMVTPPP RLVVGTYDSS 101 NASDSEFSDF ETSRDKSRQG PRRGKKVRKM PVSYLGSKFL GSDLESEDDE 151 ELVEAFLRRQ EKQPSAPPAR RRVNLPVPMF EDNLGPQLSK ADRWREYVSQ 201 VSWGKLKRRV KGWAPRAGPG VGEARLASTA VESAGVSSAP EGTSPGDRLG 251 NAGDVCVPQA SPRRWRPKIN WASFRRRRKE QTAPTGQGAD IEADQGGEAA 301 DSQREEAIAD QREGAAGNQR AGAPADQGAE AADNQREEAA DNQRAGAPAE 351 EGAEAADNQR EEAADNQRAE APADQRSQGT DNHREEAADN QRAEAPADQG 401 SEVTDNQREE AVHDQRERAP AVQGADNQRA QARAGQRAEA AHNQRAGAPG 451 IQEAEVSAAQ GTTGTAPGAR ARKQVKTVRF QTPGRFSWFC KRRRAFWHTP 501 RLPTLPKRVP RAGEVRNLRV LRAEARAEAE QGEQEDQL

BLASTP hits

Entry RNU67136_1 from database TREMBL:

"A-kinase anchoring protein AKAP150"; Rattus norvegicus

A-kinase anchoring protein AKAP150 mRNA, complete eds. Rattus norvegicus (Norway rat)

Length = 714

Score = 182 (64.1 bits), Expect = 1.2e-10, P = 1.2e-10

Identities = 73/257 (28%), Positives = 104/257 (40%)

Alert BLASTP hits for DKFZphfbr2_22k3, frame 2

TREMBL :PFSANTY_1 product: "S-antigen"; Plasmodium falciparum KF1916 S-antigen gene, complete eds., N = 1, Score = 178, P = 3.7e-ll

>TREMBL:PFSANTY_1 product: "S-antigen"; Plasmodium falciparum KF1916 S-antigen gene, complete eds. Length = 285

HSPs:

Score = 178 (26.7 bits). Expect = 3.7e-ll, P = 3.7e-ll Identities = 60/217 (27%), Positives = 97/217 (44%)

Query: 269 INWASFRRRRKEQTAPTGQGA-DIEADQGGEAADSQRE-EAIADQ REGAAGNQRAGA 323

+N + + + E G+G D E E +D+ E E I Q E A N+ AG+ Sbjct: 47 LNGKNGKGNKYEDLQEEGEGENDDEEHSNSEESDNDEENEIIVGQDGSNEKAGSNEEAGS 106

Query: 324 PADQGAEAADNQREEAADNQRAGAPAEEGA—EAADNQR EEAADNQRAEAPADQRS 377

G+ E+A N++AG+ E G+ EA N+ EEA N++A + S Sbjct: 107 NEKAGSNEEAGSNEKAGSNEKAGSNEEAGSNEEAGSNEEAGSNEEAGSNEKAGSNEKAGS 166

Query: 378 QGTDNHREEAADNQRAEAPADQGSEVTDNQREEAVHDQRERAPAVQGADNQRAQAR—AG 435

EEA N++A + + GS E+A +++ + G+ N++A + AG Sbjct: 167 NEKAGSNEEAGSNEKAGSNEEAGSNEKAGSNEKAGSNEKAGSNEEAGS-NEKAGSNEEAG 225

Query: 436 QRAEAAHNQRAGA PGIQEAEVSAAQGTTGTA-PGA 469 EA N+ AG+ G E + +G GT PG+ Sbjct: 226 SNEEAGSNEEAGSNEEAGSNEGSEAGTEGPKGTGGPGS 263

Score = 173 (26.0 bits), Expect = 1.5e-10, P = 1.5e-10 Identities = 51/190 (26%), Positives = 83/190 (43%)

Query: 279 KEQTAPTGQ-GADIEADQGGEAADSQREEAIADQREGAAGNQRAGAPADQGAEAADNQRE 337

+E GQ G++ +A EA +++ A E A N++AG+ G+ E Sbjct: 83 EENEIIVGQDGSNEKAGSNEEAGSNEK AGSNEEAGSNEKAGSNEKAGSNEEAGSNE 138

Query: 338 EAADNQRAGAPAEEGAEAADNQREEAADNQRAEAPADQRSQGTDNHREEAADNQRAEAPA 397

EA N+ AG+ E G+ E+A N++A + + S EEA N++A + Sbjct: 139 EAGSNEEAGSNEEAGSNEKAGSNEKAGSNEKAGSNEEAGSNEKAGSNEEAGSNEKAGSNE 198

Query: 398 DQGSEVTDNQREEAVHDQRERAPAVQGADNQRAQARAGQRAEAAHNQRAGAPGIQEAEVS 457

GS EEA +++ + G++ + AG EA N+ AG+ EA Sbjct: 199 KAGSNEKAGSNEEAGSNEKAGSNEEAGSNEE AGSNEEAGSNEEAGSNEGSEAGTE 253

Query: 458 AAQGTTGTAPG 468

+GT G G Sbjct: 254 GPKGTGGPGSG 264

Score = 147 (22.1 bits), Expect = 1.6e-07, P = 1.6e-07 Identities = 40/168 (23%), Positives = 70/168 (41%)

Query: 288 GADIEADQGGEAADSQR—EEAIADQREGAAGNQRAGAPADQGAEAADNQREEAADNQRA 345

G++ EA +A +++ A E A N+ AG+ + G+ E+A N++A Sbjct: 111 GSNEEAGSNEKAGSNEKAGΞNEEAGSNEEAGSNEEAGSNEEAGSNEKAGSNEKAGSNEKA 170

Query: 346 GAPAEEGAEAADNQREEAADNQRAEAPADQRSQGTDNHREEAADNQRAEAPADQGSEVTD 405

G+ E G+ EEA N++A + S EEA N++A + + GS Sbjct: 171 GSNEEAGSNEKAGSNEEAGSNEKAGSNEKAGSNEKAGSNEEAGSNEKAGSNEEAGSNEEA 230

Query: 406 NQREEAVHDQR—ERAPAVQGADNQRAQARAGQRAEAAHNQRAGAPGI 451

EEA ++ + G + + G E +HN++ I Sbjct: 231 GSNEEAGSNEEAGSNEGSEAGTEGPKGTGGPGSGGEHSHNKKKSKKSI 278

Score = 101 (15.2 bits), Expect = 2.5e-02, P = 2.4e-02 Identities = 26/100 (26%), Positives = 47/100 (47%)

Query: 281 QTAPTGQGADIEADQGGEAADSQREEAIADQREGAAGNQRAGAPADQGAEAADNQREEAA 340

+ A + + A + G EEA ++++ G+ N++AG+ G+ E+A Sbjct: 162 EKAGSNEKAGSNEEAGSNEKAGSNEEAGSNEKAGS--NEKAGSNEKAGSNEEAGSNEKAG 219

Query: 341 DNQRAGAPAEEGAEAADNQREEAADNQRAEAPADQRSQGT 380

N+ AG+ E G+ EEA N+ +EA + +GT Sbjct: 220 SNEEAGΞNEEAGSNEEAGSNEEAGSNEGSEA-GTEGPKGT 258

Pedant information for DKFZphfbr2_22k3, frame 2

Report for DKFZphfbr2_22 3.2

[LENGTH] 538

[MW] 59402.19

[pi] 8.72

[HOMOL] TREMBL:AF037364_1 gene: "MAI"; product: "paraneoplastic neuronal antigen MAI"

Homo sapiens paraneoplastic neuronal antigen MAI (MAI) mRNA, complete eds. 4e-10

[PROSITE] AMIDATION 1

[PROSITE] MYRISTYL 12

[PROSITE] CK2_PHOSPHO_SITE 11

[PROSITE] PKC_PHOSPHO_SITE 6

[PROSITE] ASN_GLYCOSYLATION 1

[KW] All_Alpha

[KW] LOW_COMPLEXITY 18.03 %

SEQ MLQIGEDVDYLLIPREVRLAGGVWRVISKPATKEAEFRERLTQFLEEEGRTLEDVARIME SEG

PRD cccccccccccccccccccccceeeeeeecccchhhhhhhhhhhhhhhccchhhhhhhhh

SEQ KSTPHPPQPPKKPKEPRVRRRVQQMVTPPPRLVVGTYDSΞNASDSEFΞDFETΞRDKSRQG SEG xxxxxxxxxxxxxxxxxxx

PRD hcccccccccccccccchhhhhhhhhccccceeeeecccccccccccccccccccccccc

SEQ PRRGKKVRKMPVSYLGSKFLGSDLESEDDEELVEAFLRRQEKQPSAPPARRRVNLPVPMF SEG xxxxxxxxxxx

PRD ccccccccccceeeccccccccccccchhhhhhhhhhhhhhccccccchhhhhccccccc SEQ EDNLGPQLSKADRWREYVSQVSWGKLKRRVKGWAPRAGPGVGEARLASTAVESAGVSSAP SEG PRD cccccccchhhhhhhhhheeeeccchhhhhhccccccccccchhhhhhhhhhhccccccc

SEQ EGTSPGDRLGNAGDVCVPQAΞPRRWRPKINWASFRRRRKEQTAPTGQGADIEADQGGEAA SEG PRD cccccccccccccceeeecccccccccccchhhhhhhhhhhhhcccccchhhhhccchhh

SEQ DSQREEAIADQREGAAGNQRAGAPADQGAEAADNQREEAADNQRAGAPAEEGAEAADNQR SEG xxxxxxxxxxxxx xxxxxxxxxxxx.... PRD hhhhhhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhccccchhhhhhhhhhh

SEQ EEAADNQRAEAPADQRSQGTDNHREEAADNQRAEAPADQGSEVTDNQREEAVHDQRERAP SEG PRD hhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ AVQGADNQRAQARAGQRAEAAHNQRAGAPGIQEAEVSAAQGTTGTAPGARARKQVKTVRF

SEG xxxxxxxxxxxxxx xxxxxxxxxxxxxx

PRD hhccchhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhccccccccchhhhhhhhhhh

SEQ QTPGRFSWFCKRRRAFWHTPRLPTLPKRVPRAGEVRNLRVLRAEARAEAEQGEQEDQL

SEG xxxxxxxxxxxxxx ...

PRD cccccceeehhhhhhhccccccccccccccccccchhhhhhhhhhhhhhhhhhhhccc

Prosite for DKFZphfbr2_22k3.2

PS00001 101->105 ASN_GLYCOSYLATION PDOC00001 PS00005 112->115 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 261->264 PKC_PHOSPHO_SITE PDOC00005 PS00005 ₂73->276 PKC_PHOSPHO_SITE PDOC00005 PS00005 302->305 PKC_PHOSPHO_SITE PDOC00005 PS00005 477->480 PKC_PHOSPHO_SITE PDOC00005 PS00005 499->502 PKC_PHOSPHO_SITE PDOC00005 PS00006 51->55 CK2_PHOΞPHO_SITE PDOC00006 PS00006 103->107 CK2_PHOSPHO_SITE PDOC00006 PS00006 108->112 CK2_PHOΞPHO_SITE PDOC00006 PS00006 112->116 CK2_PHOSPHO_SITE PDOC00006 PS00006 142->146 CK2_PHOSPHO_SITE PDOC00006 PS00006 146->150 CK2_PHOSPHO_SITE PDOC00006 PS00006 189->193 CK2_PHOSPHO_SITE PDOC00006 PS00006 229->233 CK2_PHOSPHO_SITE PDOC00006 PS00006 238->242 CK2_PHOSPHO_SITE PDOC00006 PS00006 244->248 CK2_PHOSPHO_SITE PDOC00006 PS00006 302->306 CK2_PHOSPHO_SITE PDOC00006 PS00008 95->101 MYRISTYL PDOC00008 PS00008 220->226 MYRISTYL PDOC00008 PS00008 242->248 MYRISTYL PDOC00008 PΞ00008 296->30₂ MYRISTYL PDOC00008 PS00008 314->320 MYRISTYL PDOC00008 PS00008 317->323 MYRISTYL PDOC00008 PS00008 328->334 MYRISTYL PDOC00008 PS00008 352->358 MYRISTYL PDOC00008 PS00008 400->406 MYRISTYL PDOC00008 PS00008 450->456 MYRISTYL PDOC00008 PS00008 461->467 MYRISTYL PDOC00008 PS00008 464->470 MYRISTYL PDOC00008 PS00009 123-M27 AMIDATION PDOC00009

(No Pfam data available for DKFZphfbr2_22k3.2)

DKFZphfbr2_22k8

group: brain derived

DKFZphfbr2_22k8 encodes a novel 172 amino acid protein without similarity to known proteins.

No informative BLAST results; no predictive prosite, pfam or SCOP motife

The new protein can find application studying the expression profile of bram-specific genes . unknown complete cDNA, complete eds, EST hits

Sequenced by AGOWA

Locus: /map="7"

Insert length: 2789 bp

Poly A stretch at pos. 2769, polyadenylation signal at pos. 2756

1 GGGGGAGCCA TGAGGCGCCA GCCTGCGAAG GTGGCGGCGC TGCTGCTCGG

51 GCTGCTCTTG GAGTGCACAG AAGCCAAAAA GCATTGCTGG TATTTCGAAG

101 GACTCTATCC AACCTATTAT ATATGCCGCT CCTACGAGGA CTGCTGTGGC

151 TCCAGGTGCT GTGTGCGGGC CCTCTCCATA CAGAGGCTGT GGTACTTCTG

201 GTTCCTTCTG ATGATGGGCG TGCTTTTCTG CTGCGGAGCC GGCTTCTTCA

251 TCCGGAGGCG CATGTACCCC CCGCCGCTGA TCGAGGAGCC AGCCTTCAAT

301 GTGTCCTACA CCAGGCAGCC CCCAAATCCC GGCCCAGGAG CCCAGCAGCC

351 GGGGCCGCCC TATTACACTG ACCCAGGAGG ACCGGGGATG AACCCTGTCG

401 GGAATTCCAC GGCAATGGCT TTCCAGGTCC CACCCAACTC ACCCCAGGGG

451 AGTGTGGCCT GCCCGCCCCC TCCAGCCTAC TGCAACACGC CTCCGCCCCC

501 GTACGAACAG GTAGTGAAGG CCAAGTAGTG GGGTGCCCAC GTGCAAGAGG

551 AGAGACAGGA GAGGGCCTTT CCCTGGCCTT TCTGTCTTCG TTGATGTTCA

601 CTTCCAGGAA CGGTCTCGTG GGCTGCTAAG GGCAGTTCCT CTGATATCCT

651 CACAGCAAGC ACAGCTCTCT TTCAGGCTTT CCATGGAGTA CAATATATGA

701 ACTCACACTT TGTCTCCTCT GTTGCTTCTG TTTCTGACGC AGTCTGTGCT

751 CTCACATGGT AGTGTGGTGA CAGTCCCCGA GGGCTGACGT CCTTACGGTG

801 GCGTGACCAG ATCTACAGGA GAGAGACTGA GAGGAAGAAG GCAGTGCTGG

851 AGGTGCAGGT GGCATGTAGA GGGGCCAGGC CGAGCATCCC AGGCAAGCAT

901 CCTTCTGCCC GGGTATTAAT AGGAAGCCCC ATGCCGGGCG GCTCAGCCGA

951 TGAAGCAGCA GCCGACTGAG CTGAGCCCAG CAGGTCATCT GCTCCAGCCT

1001 GTCCTCTCGT CAGCCTTCCT CTTCCAGAAG CTGTTGGAGA GACATTCAGG

1051 AGAGAGCAAG CCCCTTGTCA TGTTTCTGTC TCTGTTCATA TCCTAAAGAT

1101 AGACTTCTCC TGCACCGCCA GGGAAGGATA GCACGTGCAG CTCTCACCGC

1151 AGGATGGGGC CTAGAATCAG GCTTGCCTTG GAGGCCTGAC AGTGATCTGA

1201 CATCCACTAA GCAAATTTAT TTAAATTCAT GGGAAATCAC TTCCTGCCCC

1251 AAACTGAGAC ATTGCATTTT GTGAGCTCTT GGTCTGATTT GGAGAAAGGA

1301 CTGTTACCCA TTTTTTTGGT GTGTTTATGG AAGTGCATGT AGAGCGTCCT

1351 GCCCTTTGAA ATCAGACTGG GTGTGTGTCT TCCCTGGACA TCACTGCCTC

1401 TCCAGGGCAT TCTCAGGCCC GGGGGTCTCC TTCCCTCAGG CAGCTCCAGT

1451 GGTGGGTTCT GAAGGGTGCT TTCAAAACGG GGCACATCTG GCCGGGAAGT

1501 CACATGGACT CTTCCAGGGA GAGAGACCAG CTGAGGCGTC TCTCTCTGAG

1551 GTTGTGTTGG GTCTAAGCGG GTGTGTGCTG GGCTCCAAGG AGGAGGAGCT

1601 TGCTGGGAAA AGACAGGAGA AGTACTGACT CAACTGCACT GACCATGTTG

1651 TCATAATTAG AATAAAGAAG AAGTGGTCGG AAATGCACAT TCCTGGATAG

1701 GAATCACAGC TCACCCCAGG ATCTCACAGG TAGTCTCCTG AGTAGTTGAC

1751 GGCTAGCGGG GAGCTAGTTC CGCCGCATAG TTATAGTGTT GATGTGTGAA

1801 CGCTGACCTG TCCTGTGTGC TAAGAGCTAT GCAGCTTAGC TGAGGCGCCT

1851 AGATTACTAG ATGTGCTGTA TCACGGGGAA TGAGGTGGGG GTGCTTATTT

1901 TTTAATGAAC TAATCAGAGC CTCTTGAGAA ATTGTTACTC ATTGAACTGG

1951 AGCATCAAGA CATCTCATGG AAGTGGATAC GGAGTGATTT GGTGTCCATG

2001 CTTTTCACTC TGAGGACATT TAATCGGAGA ACCTCCTGGG GAATTTTGTG

2051 GGAGACACTT GGGAACAAAA CAGACACCCT GGGAATGCAG TTGCAAGCAC

2101 AGATGCTGCC ACCAGTGTCT CTGACCACCC TGGTGTGACT GCTGACTGCC

2151 AGCGTGGTAC CTCCCATGCT GCAGGCCTCC ATCTAAATGA GACAACAAAG

2201 CACAATGTTC ACTGTTTACA ACCAAGACAA CTGCGTGGGT CCAAACACTC

2251 CTCTTCCTCC AGGTCATTTG TTTTGCATTT TTAATGTCTT TATTTTTTGT

2301 AATGAAAAAG CACACTAAGC TGCCCCTGGA ATCGGGTGCA GCTGAATAGG

2351 CACCCAAAAG TCCGTGACTA AATTCCGTTT GTCTTTTTGA TAGCAAATTA

2401 TGTTAAGAGA CAGTGATGGC TAGGGCTCAA CAATTTTGTA TTCCCATGTT

₂451 TGTGTGAGAC AGAGTTTGTT TTCCCTTGAA CTTGGTTAGA ATTGTGCTAC

₂501 TGTGAACGCT GATCCTGCAT ATGGAAGTCC CACTTTGGTG ACATTTCCTG

2551 GCCATTCTTG TTTCCATTGT GTGGATGGTG GGTTGTGCCC ACTTCCTGGA

2601 GTGAGACAGC TCCTGGTGTG TAGAATTCCC GGAGCGTCCG TGGTTCAGAG

2651 TAAACTTGAA GCAGATCTGT GCATGCTTTT CCTCTGCAGC AATTGGCTCG

2701 TTTCTCTTTT TTGTTCTCTT TTGATAGGAT CCTGTTTCCT ATGTGTGCAA 2751 AATAAAAATA AATTTGGGCA AAAAAAAAAA AAAAAAAAA

BLAST Results

Entry HS671255 from database EMBL: human STS SHGC-11828.

Length = 400

Minus Strand HSPs:

Score = 1822 (273.4 bits), Expect = 4.8e-76, P = 4.8e-76

Identities = 382/397 (96%), Positives = 382/397 (96%),

Medlme entries

No Medlme entry

Peptide information for frame 1

ORF from 10 bp to 525 bp; peptide length: 172 Category: putative protein Classification: unset

1 MRRQPAKVAA LLLGLLLECT EAKKHCWYFE GLYPTYYICR SYEDCCGSRC

51 CVRALSIQRL WYFWFLLMMG VLFCCGAGFF IRRRMYPPPL IEEPAFNVSY

101 TRQPPNPGPG AQQPGPPYYT DPGGPGMNPV GNSTAMAFQV PPNSPQGSVA

151 CPPPPAYCNT PPPPYEQVVK AK

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_22k8, frame 1

PIR:S14970 extensm class I (clone wl7-l) - tomato, N = 1, Score = 118, P = 2.3e-07

>PIR:S14970 extensin class I (clone wl7-l) - tomato Length = 132

HSPs:

Score = 118 (17.7 bits), Expect = 2.3e-07, P = 2.3e-07 Identities = 30/82 (36%), Positives = 35/82 (42%)

Query: 87 PPPLIEEPAFNVSYTRQPPNPGPGAQQPGPPYYTDPGGPGMNPVGNSTAMAFQVPPNSPQ 146

PPP P Y + PP P P P P YY P P +P + P SP Sbjct: 32 PPPSPSPPP—PYYYKSPPPPSPSP—PPPYYYKSPPPPDPSPPPPYYYKSPPPPSPSPP 87

Query: 147 GSVACPPPPAYCNTPPPP— EQV 168

PPPP Y + PPPP YE + Sbjct: 88 PPSPSPPPPTYSSPPPPPPFYENI 111

Score = 104 (15.6 bits), Expect = 6.9e-06, P = 6.9e-06 Identities = 28/78 (35%), Positives = 34/78 (43%)

Query: 87 PPPLIEEPAFNVSYTRQPPNPGPGAQQPGPPYYTDPGGPGMNPVGNSTAMAFQVPPNSPQ 146

PP P + Y + PP P P P P YY P P +P ++ PP P Sbjct: 1 PPSPSPPPPY YYKΞPPPPSPSP—PPPYYYKSPPPPSPSP PPPYYYKSPP-PPS 51

Query: 147 GSVACPPPPAYCNTPPPP 164

S PPPP Y +PPPP Sbjct: 52 PS PPPPYYYKSPPPP 66

Score = 102 (15.3 bits), Expect = l.le-05, P = l.le-05 Identities = 30/78 (38%), Positives = 33/78 (42%)

Query: 87 PPPLIEEPAFNVSYTRQPPNPGPGAQQPGPPYYTDPGGPGMNPVGNSTAMAFQVPPNSPQ 146

PPP P Y + PP P P P P YY P P +P S + PP P Sbjct: 48 PPPSPSPPP—PYYYKSPPPPDPSP—PPPYYYKSPPPPSPSPPPPSPS PP-PPT 97 Query: 147 GSVACPPPPAYCNTPPPP 164

S PPPP Y N P PP Sbjct: 98 YSSPPPPPPFYENIPLPP 115

Score = 95 (14.3 bits), Expect = 2.4e-04, P = 2.4e-04 Identities = 24/61 (39%), Positives = 29/61 (47%)

Query 104 PPNPGPGAQQPGPPYYTDPGGPGMNPVGNSTAMAFQVPPNSPQGSVACPPPPAYCNTPPP 163

PP+P P P P YY P P +P ++ PP P S PPPP Y +PPP Sbjct 1 PPSPSP PPPYYYKSPPPPSPSP PPPYYYKSPP-PPSPS PPPPYYYKSPPP 49 Query 164 P 164

P Sbjct 50 P 50

Score = 68 (10.2 bits), Expect = 4.2e+00, P = 9.8e-01 Identities = 24/69 (34%), Positives = 29/69 (42%)

Query: 87 PPPLIEEPAFNVSYTRQPP NPGPGAQQPGPPYYTDPGGPGMNPVGNSTAMAFQVPPN 143

PPP P Y PP +P P + P PP Y+ P P P + + PP Sbjct: 63 PPPPDPSPPPPYYYKSPPPPSPSPPPPSPSPPPPTYSSPPPPP—PFYENIPL PPV 116

Query: 144 SPQGSVACPPPP 155

S A PPPP Sbjct: 117 IGV-SYASPPPP 127

Peptide information for frame 3

ORF from 0 bp to 368 bp; peptide length: 123 Category: questionable ORF Classification: unset

1 GSHEAPACEG GGAAARAALG VHRSQKALLV FRRTLSNLLY MPLLRGLLWL 51 QVLCAGPLHT EAVVLLVPSD DGRAFLLRSR LLHPEAHVPP AADRGASLQC 101 VLHQAAPKSR PRSPAAGAAL LH

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_22k8, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_22k8, frame 1

Report for DKFZphfbr2_22k8.1

[LENGTH] 172

[MW] 19194.47

[pi] 8.77

[KW] SIGNAL_PEPTIDE 23

[KW] TRANSMEMBRANE 1

[KW] LOW_COMPLEXITY 27.33 %

SEQ MRRQPAKVAALLLGLLLECTEAKKHCWYFEGLYPTYYICRSYEDCCGSRCCVRALΞIQRL

SEG xxxxxxx

PRD ccchhhhhhhhhhhhhhhhhhhhhhcccccccccceeeeccccccccccchhhhhhhhhh

MEM

SEQ WYFWFLLMMGVLFCCGAGFFIRRRMYPPPLIEEPAFNVSYTRQPPNPGPGAQQPGPPYYT

SEG xxxxxxxxxxxxxxxxxx

PRD hhhhhhhhhhhhhccccceeeeecccccccccccccceeeeccccccccccccccccccc

MEM .... MMMMMMMMMMMMMMMMM

SEQ DPGGPGMNPVGNSTAMAFQVPPNSPQGSVACPPPPAYCNTPPPPYEQVVKAK

SEG xxxxxx xxxxxxxxxxxxxxxx

PRD ccccccccccccccceeecccccccccccccccccccccccccccccccccc

MEM

(No Prosite data available for DKFZphfbr2_22k8.1) (No Pfam data available for DKFZphfbr2_22k8.1)

Pedant information for DKFZphfbr2_22k8, frame 3

Report for DKFZphfbr2_22k8.3

[LENGTH] 122

[MW] 12854.08

[pi] 10.27

[KW] All_Alpha

[KW] LOW_COMPLEXITY 25.41 %

SEQ GSHEAPACEGGGAAARAALGVHRΞQKALLVFRRTLSNLLYMPLLRGLLWLQVLCAGPLHT SEG .... xxxxxxxxxxxxxxxx

PRD ccccccccccchhhhhhhhccccchhhhhhhhhhhhhhhccccccchhhhhhhhcccccc

SEQ EAVVLLVPSDDGRAFLLRSRLLHPEAHVPPAADRGASLQCVLHQAAPKSRPRSPAAGAAL

SEG xxxxxxxxxxxxxxx .

PRD cceeeeeccccchhhhhhhhccccccccccccccchhhhhhhhhccccccccchhhhhhc

SEQ LH

SEG

PRD cc

(No Prosite data available for DKFZphfbr2_22k8.3) (No Pfam data available for DKFZphfbr2_22k8.3)

DKFZphfbr2_23blO

group: nucleic acid managment

DKFZphfbr2_2blO encodes a novel 580 amino acid protein with strong similarity to rat RNA helicase HEL117.

HEL117 is a DEAD/H box helicase, which co-localises with a splicing factor and thus seems to be involved in splicing.

The new protein can find application in modulation of splicing. strong similarity to rat RNA helicase HEL117 complete cDNA, complete eds, EST hits

Sequenced by AGOWA

Locus: unknown

Insert length: 2905 bp

Poly A stretch at pos. 2885, no polyadenylation signal found

1 GGGGGCTCCG CTCCGCACCA CCAACCCCGG GCCGCAGTCC TGACGAGCGG

51 GTCAGGGCTT GTCGGGCGGA AGCCTGGCCT GGAGCCTGGA AGGGGGAGAC

101 GGCCCGAGCG GGAGCGGGAG CGGACGCGGC CTCAGTCCTG CGCGGAATAT

151 TGAAGGATGT TTGTTCCAAG ATCTCTAAAA ATCAAGAGGA ATGCTAATGA

201 TGATGGCAAA AGTTGTGTGG CTAAGATAAT TAAACCAGAC CCAGAAGACC

251 TTCAGTTGGA CAAAAGCAGA GATGTTCCCG TTGATGCTGT AGCTACAGAA

301 GCAGCCACAA TAGACAGGCA CATCAGCGAA TCATGCCCTT TCCCCAGCCC

351 AGGTGGCCAG TTGGCAGAGG TTCATTCAGT AAGTCCCGAG CAGGGTGCGA

401 AGGACAGCCA TCCTTCTGAA GAGCCCGTTA AGTCATTTTC CAAAACACAG

451 CGCTGGGCAG AACCAGGGGA ACCCATCTGT GTTGTCTGTG GTCGTTATGG

501 AGAGTATATC TGTGATAAGA CAGATGAAGA TGTGTGTAGT TTGGAGTGTA

551 AAGCGAAACA TCTTCTACAA GTTAAGGAAA AGGAAGAGAA ATCAAAACTC

601 AGCAATCCAC AGAAGGCTGA TTCTGAGCCA GAGTCTCCAC TGAATGCTTC

651 CTATGTCTAC AAAGAGCACC CCTTTATTTT GAACCTTCAG GAAGACCAGA

701 TTGAAAATCT TAAACAGCAG CTGGGAATTT TAGTTCAAGG GCAAGAAGTC

751 ACCAGGCCCA TTATTGACTT TGAACATTGT AGTCTCCCTG AGGTCTTAAA

801 TCACAACTTG AAGAAATCAG GCTATGAGGT GCCAACTCCC ATTCAAATGC

851 AGATGATTCC TGTGGGACTT CTGGGAAGAG ACATTCTGGC CAGTGCAGAT

901 ACTGGCTCAG GAAAAACAGC TGCTTTTCTT CTTCCTGTTA TCATGCGAGC

951 TTTATTCGAG AGCAAAACTC CATCTGCGCT CATTCTTACA CCAACCAGAG

1001 AGTTAGCCAT TCAGATAGAG AGACAAGCTA AAGAATTGAT GAGTGGCCTG

1051 CCACGCATGA AAACTGTGCT TCTTGTAGGG GGCTTACCCT TACCCCCACA

1101 GCTTTATCGT CTGCAACAAC ATGTTAAGGT TATCATAGCA ACCCCTGGGC

1151 GACTTCTGGA TATAATAAAG CAGAGCTCTG TAGAACTCTG TGGTGTAAAG

1201 ATTGTGGTAG TAGATGAAGC TGATACCATG TTAAAGATGG GTTTTCAACA

1251 ACAAGTGCTT GACATTTTGG AAAACATTCC TAATGATTGT CAGACCATTT

1301 TGGTTTCAGC CACAATTCCA ACTAGCATAG AACAGCTAGC AAGCCAGCTT

1351 CTGCATAATC CTGTGAGAAT TATCACTGGA GAAAAGAACC TACCTTGTGC

1401 CAATGTACGT CAGATTATTT TGTGGGTAGA AGACCCAGCC AAAAAGAAAA

1451 AATTATTTGA AATTTTAAAT GATAAGAAAC TCTTTAAGCC TCCAGTGTTA

1501 GTATTTGTGG ACTGCAAACT AGGAGCAGAT CTTTTGAGTG AAGCCGTTCA

1551 GAAAATCACA GGGCTGAAAA GCATATCTAT ACATTCGGAG AAGTCGCAAA

1601 TAGAAAGGAA AAACATATTG AAGGGATTAC TTGAAGGAGA CTATGAAGTT

1651 GTAGTGAGCA CAGGAGTCTT GGGACGAGGC CTAGACTTGA TCAGTGTCAG

1701 GCTGGTTGTC AATTTTGATA TGCCTTCAAG TATGGATGAG TATGTCCATC

1751 AGGAAAATAC CTACAAGTCT ACTTGGAGGA ATCCCCAGCA TTTTCAACAG

1801 GATGTCAGAA TGACCTTGGG CTATGTTGGC AAAGCACAAT GGGAAGAAGA

1851 CAACCAATTG AAGGTCAAAC TAGGCCTTAA AAAAAATTGT TCTTCCTAAA

1901 TGAAACTTTA TGTAAGACCC AAGCTTCCTT TATGTAAAAA TAGGATACTC

1951 ACTAGGCTTT GGGGCTGACA ATGGTTTTTA AATCTTGCTA ATCTTCCCTG

2001 GAATGAAACC AGCATGACTC AAAGAGAAAA AGAGAGTCTA TAATATTTTC

2051 TAATCCCTGA GTTCTTTTCT TTATATATTA AAAAGGATTA TTAGGCTGGG

2101 TGTGGTGGCT CACGCCTGTA ATCCCAGCAC TTTGGGAGGC CGAGGGGAGT

2151 GGATCACCTG AGTTCGAGAC CAGCCTAACC AACATGGAGA AACCCTGTCT

2201 CTACTAAAAA TACAAAATTA GCCAGGCGTG GTGGCGCATG CCTGTAATCC

2251 CAGCTACTCA GGAGGCTACA GCAGGAGAAT TGCTTGAACT CGGGAGGCAG

2301 AGCCAAGATC GCACCACTGC ACTCCAGCCT GGGCAACAAG AGTGAAACTC

2351 TGTCTCAAAA TAATATTAAT GATAATAATA ATAATAATAA TAGGGATTAC

2401 TTGCATAATT GTTCTTTTAA AATTATTGGC AGTATTGCTG AATGTATTTA

2451 GATTTTTTCA CCAAGTGACA ACAACTGAAT TCATAAAGAT TCATCAACAA

2501 GACCTGATAA AAAAAAATGT AAGCATATTA TAGTGGATAC TTCCAAGACT

2551 CTTGGTCTAA CATGTATTAG AAAGCAGAAG GAGCCCAGGC ACAGGGGCTC

2601 CCGCCGGTAA TCCCAAAGCT TTGGGAAGCC AAGGCAGGTG GATCGCTTGA

2651 GCTCAGGAGT TAGAGACCAG CCTGGGCAAC ATGGTGAAAT CCCGTCACCA 2701 CAAAAAAATG CAAAAATTAA CTGGGCGTGG TGGCATGCAC CTGTAGTCCC 2751 AGCTACTCTG GAGGCTGAGG TGAGGGGAAT CACCTGAGCC GGGGGAATCA 2801 CCTGAGCCCA GGGAAGTTGA GGCTGCTGTG AGCCATGGTC ATGACACTGC 2851 CCTCCAGCCT GGACAACAGA TTGAGACCCT GTCTCAAAAA AAAAAAAAAA 2901 AAAAA

BLAST Results

No BLAST result

Medlme entries

Medline:

A putative mammalian RNA helicase with an arginme-serine-rich domain

Peptide information for frame 1

ORF from 157 bp to 1896 bp; peptide length: 580 Category: strong similarity to known protein Prosite motifs: ATP_GTP_A (247-255) LEUCINE ZIPPER (298-320)

1 MFVPRSLKIK RNANDDGKSC VAKIIKPDPE DLQLDKSRDV PVDAVATEAA

51 TIDRHISESC PFPSPGGQLA EVHSVSPEQG AKDSHPSEEP VKSFSKTQRW

101 AEPGEPICVV CGRYGEYICD KTDEDVCSLE CKAKHLLQVK EKEEKSKLSN

151 PQKADSEPES PLNASYVYKE HPFILNLQED QIENLKQQLG ILVQGQEVTR

201 PIIDFEHCSL PEVLNHNLKK SGYEVPTPIQ MQMIPVGLLG RDILASADTG

251 SGKTAAFLLP VIMRALFESK TPSALILTPT RELAIQIERQ AKELMSGLPR

301 MKTVLLVGGL PLPPQLYRLQ QHVKVIIATP GRLLDIIKQS SVELCGVKIV

351 VVDEADTMLK MGFQQQVLDI LENIPNDCQT ILVSATIPTΞ IEQLASQLLH

401 NPVRIITGEK NLPCANVRQI ILWVEDPAKK KKLFEILNDK KLFKPPVLVF

451 VDCKLGADLL SEAVQKITGL KSISIHSEKS QIERKNILKG LLEGDYEVVV

501 STGVLGRGLD LISVRLVVNF DMPSSMDEYV HQENTYKSTW RNPQHFQQDV

551 RMTLGYVGKA QWEEDNQLKV KLGLKKNCSS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_23blO, frame 1

PIR:A57514 RNA helicase HEL117 - rat, N = 2, Score = 615, P = 1.6e-60

TREMBL:AB018344_1 gene: "KIAA0801"; product: "KIAA0801 protein"; Homo sapiens mRNA for KIAA0801 protein, complete eds., N = 1, Score = 615, P = 2.8e-59

TREMBL :CEF01F1_1 gene: "F01F1.7"; Caenorhabditis elegans cosmid F01F1., N = 2, Score = 365, P = 1.9e-58

TREMBL:AF083255_1 product: "RNA helicase-related protein"; Homo sapiens RNA helicase-related protein mRNA, complete eds., N = 2, Score = 556, P = 1.5e-57

PIR:S14048 RNA helicase dbp2 - fission yeast (Schizosaccharomyces pombe), N = 1, Score = 591, P = 1.6e-57

>PIR:A57514 RNA helicase HEL117 - rat Length = 1,032

HSPs:

Score = 615 (92.3 bits), Expect = 1.6e-60, Sum P(2) = 1.6e-60 Identities = 140/394 (35%), Positives = 236/394 (59%)

Query: 144 EKSKLSNPQKADSEPESPLNASYVYKEHPFILNLQEDQIENLKQQL-GILVQGQEVTRPI 202

++ KL P P ++ Y E P + + ++++ + ++ GI V+G+ +PI

Sbjct: 313 KQRKLLEPVDHGKIEYEPFRKNF-YVEVPELAKMSQEEVNVFRLEMEGITVKGKGCPKPI 371

Query: 203 IDFEHCSLPEVLNHNLKKSGYEVPTPIQMQMIPVGLLGRDILASADTGSGKTAAFLLPV- 261 + C + + ++LKK GYE PTPIQ Q IP + GRD++ A TGSGKT AFLLP+ Sbjct: 372 KSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMF 431

Query: 262 --IM—RALFESKTPSALILTPTRELAIQIERQAKELMSGLPRMKTVLLVGGLPLPPQLY 317

IM R+L E + P A+I+TPTRELA+QI ++ K+ L ++ V + GG + Q+ Sbjct: 432 RHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLG-LRVVCVYGGTGISEQIA 490

Query: 318 RLQQHVKVIIATPGRLLDIIKQSΞ VELCGVKIVVVDEADTMLKMGFQQQVLDILENI 374

L++ ++I+ TPGR++D++ +S L V VV+DEAD M MGF+ QV+ I++N+ Sbjct: 491 ELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNV 550

Query: 375 PNDCQTILVSATIPTSIEQLASQLLHNPVRIITGEKNLPCANVRQIILWVEDPAKKKKLF 434

D QT++ SAT P ++E LA ++L P+ + G +++ C++V Q ++ +E+ K KL Sbjct: 551 RPDRQTVMFSATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQQVIVIEEEKKFLKLL 610

Query: 435 EILNDKKLFKPPVLVFVDCKLGADLLSEAVQKITGLKSISIHSEKSQIERKNILKGLLEG 494

E+L + V++FVD + AD L + + + + +S+H Q +R +1+ G Sbjct: 611 ELLGHYQE-SGSVIIFVDKQEHADGLLKDLMRAS-YPCMSLHGGIDQYDRDSIINDFKNG 668

Query: 495 DYEVVVSTGVLGRGLDLISVRLVVNFDMPSSMDEYVHQ 532

+++V+T V RGLD+ + LVVN+ P+ ++YVH+ Sbjct: 669 TCKLLVATSVAARGLDVKHLILVVNYSCPNHYEDYVHR 706

Score = 37 (5.6 bits), Expect = 1.6e-60, Sum P(2) = 1.6e-60 Identities = 13/36 (36%), Positives = 17/36 (47%)

Query: 132 KAKHLLQVKEKEE KSKLSNPQKADSEPESPLNA 164

KA++ + KEK E SK K D E E +A Sbjct: 113 KAENRSRSKEKAEGGDSSKEKKKDKDDKEDEKEKDA 148

Pedant information for DKFZphfbr2_23blO, frame 1

Report for DKFZphfbr2_23blO .1

[LENGTH] 580 [MW] 64572.24 [pi] 6.13 [HOMOL] TREMBL :CEF01F1 1 gene: "F01F1.7"; Caenorhabditis elegans cosmid F01F1. 8e-61

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YNL112w] 2e-53

[FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YNL112w] 2e-53

[FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YPL119c] 5e-53

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YOR204w] 2e-49

[FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae,

YOR204W] 2e-49

[ FUNCAT ] j mrna translation and ribosome biogenesis [H. influenzae, HI0231 RNA] 2e-46

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YLL008w] 3e-43

[FUNCAT] 04.99 other transcription activities [Ξ. cerevisiae, YDL160c] 4e-39

[FUNCAT] 1 genome replication, transcription, recombination and repair [H. influenzae HI0892] 3e-35

[FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YMR290c] 6e-34

[FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YOR046c] 3e-32

[FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YJL033w] 8e-30

[FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YDR194C] 5e-23

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YGL064c] le-16

[FUNCAT] r general function prediction [M. jannaschii, MJ1401] 5e-ll

[FUNCAT] 11.10 cell death [S. cerevisiae, YMR190c] le-06

[FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YMR190c] le-06

[BLOCKS] BL00115B Eukaryotic RNA polymerase II heptapeptide repeat proteins

[BLOCKS] BL00039D DEAD-box subfamily ATP-dependent helicases proteins

[BLOCKS] BL00039C DEAD-box subfamily ATP-dependent helicases proteins

[BLOCKS] BL00039B DEAD-box subfamily ATP-dependent helicases proteins

[BLOCKS] BL00039A DEAD-box subfamily ATP-dependent helicases proteins

[PIRKW] nucleus 6e-53

[PIRKW] RNA binding 9e-52

[PIRKW] DEAD box 2e-43

[PIRKW] transmembrane protein le-21

[PIRKW] DNA binding 5e-48

[PIRKW] ATP 4e-57

[PIRKW] purine nucleotide binding 2e-43

[PIRKW] P-loop 4e-57

[PIRKW] hydrolase 6e-42

[PIRKW] protein biosynthesis 2e-43

[PIRKW] ATP binding 2e-50

[SUPFAM] WW repeat homology le-49

[SUPFAM] translation initiation factor eIF-4A 2e-43

[SUPFAM] DEAD/H box helicase homology 4e-57

[SUPFAM] recQ helicase homology 8e-06 [SUPFAM] unassigned DEAD/H box helicases 4e-57

[SUPFAM] ATP-dependent RNA helicase DBP1 2e-53

[SUPFAM] ATP-dependent RNA helicase DHH1 6e-40

[SUPFAM] tobacco ATP-dependent RNA helicase DB10 le-49

[SUPFAM] Bloom's syndrome helicase 8e-06

[PROSITE] ATP_GTP_A 1

[PROSITE] LEUCINE_ZI PPER 1

[PROSITE] MYRISTYL 6

[PROSITE] CK2_PHOSPHO_SITE 8

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SI E 7

[PROSITE] ASN_GLYCOSYLATION 1

[PFAM] Helicases conserved C-termmal domain

[PFAM] DEAD and DEAH box helicases

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 3.10 %

SEQ MFVPRΞLKIKRNANDDGKSCVAKIIKPDPEDLQLDKSRDVPVDAVATEAATIDRHISEΞC SEG PRD ccccceeeeccccccccceeeeeeeeccccceeecccccccccchhhhhhhhhhhhcccc

SEQ PFPSPGGQLAEVHSVSPEQGAKDSHPSEEPVKSFSKTQRWAEPGEPICVVCGRYGEYICD SEG PRD cccccccceeeeccccccccccccccccccccccccccccccccccceeeeccccceeec

SEQ KTDEDVCSLECKAKHLLQVKEKEEKSKLSNPQKADΞEPESPLNASYVYKEHPFILNLQED SEG PRD cccccccchhhhhhhhhhhhhhccccccccccccccccccccccceeeccccccccchhh

SEQ QIENLKQQLGILVQGQEVTRPIIDFEHCSLPEVLNHNLKKSGYEVPTPIQMQMIPVGLLG SEG PRD hhhhhhhhheeeeccccccccccccccccchhhhhhhhhhhccccccccccccceeeecc

SEQ RDILASADTGΞGKTAAFLLPVIMRALFESKTPSALILTPTRELAIQIERQAKELMSGLPR SEG PRD cceeeeeccccccceeeehhhhhhhhcccccceeeeecchhhhhhhhhhhhhhhhccccc

SEQ MKTVLLVGGLPLPPQLYRLQQHVKVIIATPGRLLDIIKQSSVELCGVKIVVVDEADTMLK SEG ... xxxxxxxxxxxxxxxxx PRD eeeeeeecccccchhhhhhhhheeeeeeccccchhhhhhheeeeeeeeeeeehhhhhhhh

SEQ MGFQQQVLDILENIPNDCQTILVSATIPTSIEQLASQLLHNPVRIITGEKNLPCANVRQI SEG PRD cccchhhhhhhhhcccccceeeeecccchhhhhhhhhhhhceeeeeeeccccccccccce

SEQ ILWVEDPAKKKKLFEILNDKKLFKPPVLVFVDCKLGADLLSEAVQKITGLKSISIHSEKS SEG PRD eeecccchhhhhhhhhhhhhccccceeeeeeecccchhhhhhhhhhhhccceeeccccch

SEQ QIERKNILKGLLEGDYEVVVSTGVLGRGLDLISVRLVVNFDMPSSMDEYVHQENTYKSTW SEG PRD hhhhhhhhhhhccccceeeeehhhhhhcccceeeeeeeeecccccccceeeecccccccc

SEQ RNPQHFQQDVRMTLGYVGKAQWEEDNQLKVKLGLKKNCSS SEG PRD ccccccchhhhhhhccccchhhhhhhhhhhhhhhcccccc

Prosite for DKFZphfbr2 23bl0.1

PS00001 163->167 ASN_GLYCOSYLATION PDOC00001 PS00005 6->9 PKC_PHOΞPHO_SITE PDOC00005 PS00005 97->100 PKC_PHOSPHO_SITE PDOC00005 PS00005 251->254 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 477->480 PKC_PHOSPHO_SITE PDOC00005 PS00005 513->516 PKC_PHOSPHO_SITE PDOC00005 PS00005 535->538 PKC_PHOSPHO_SITE PDOC00005 PS00005 539->542 PKC_PHOSPHO_SITE PDOC00005 PS00006 122->126 CK2_PHOSPHO_SITE PDOC00006 PS00006 156->160 CK2_PHOSPHO_SITE PDOC00006 PS00006 209->213 CK2_PHOSPHO_SITE PDOC00006 PS00006 221->225 CK2_PHOSPHO_SITE PDOC00006 PS00006 340->344 CK2_PHOSPHO_SITE PDOC00006 PS00006 389->393 CK2_PHOSPHO_SITE PDOC00006 PS00006 480->484 CK2_PHOSPHO_SITE PDOC00006 PS00006 524->528 CK2_PHOSPHO_SITE PDOC00006 PS00007 489->497 TYR_PHOSPHO_SITE PDOC00007 PS00008 66->72 MYRISTYL PDOC00008 PS00008 80->86 MYRISTYL PDOC00008 PS00008 195->201 MYRISTYL PDOC00008 PS00008 250->256 MYRISTYL PDOC00008 PS00008 490->496 MYRISTYL PDOC00008 PS00008 573->579 MYRISTYL PDOC00008 PS00017 247->255 ATP_GTP_A PDOC00017 PS00029 298->320 LEUCINE ZIPPER PDOC00029

Pfam for DKFZphfbr2_23bl0.1

HMM_NAME DEAD and DEAH box helicases

HMM *gLpPWILRnIyeMGFEkPTPIQQqAIPHLeGRDVMACAQTGSGKTAAF +LP+ + N+++ G+E PTPIQ+Q IP+ L GRD++A A TGSGKTAAF

Query 209 SLPEVLNHNLKKSGYEVPTPIQMQMIPVGLLGRDILASADTGSGKTAAF 257

HMM HPMLQHIDwdPWpqpPQdPrALILAPTRELAMQIQEEcRkFgkHMnglR

L+P++ + + + ++P ALIL+PTRELA+QI+++++++ + ++ ++

Query 258 LLPVIMRALFES—KTPS ALILTPTRELAIQIERQAKELMSGLPRMK 302

HMM ImcIYGGtnMRdQMRmLeRGpPHIVIATPGRLIDHIERgtldLDrleMLV ++++GG+++ +Q+ +L++ + ++IATPGRL+D+I++ ++ L ++++V

Query 303 TVLLVGGLPLPPQLYRLQQHV-KVIIATPGRLLDIIKQSSVELCGVKIVV 351

HMM MDEADRMLDMGFIDQIRrIMrqlPMpwNRQTMMFSATMPdeIqELARrFM

DEAD ML MGF++Q+ +1+ IP + QT++ SAT+P +I++LA ++

Query 352 VDEADTMLKMGFQQQVLDILENIP—NDCQTILVSATIPTSIEQLASQLL 399

HMM RNPIRInldMdElTtnEnlkQwYiyVerEMWKfdcLcrLIe* +NP+RI+ ++++L N++Q++ +VE + K +L+++++

Query 400 HNPVRIITGEKNLPCA-NVRQIILWVE-DPAKKKKLFEILN 438

HMM_NAME Helicases conserved C-terminal domain

HMM *EιleeWLknl . GIrvmYIHGdMpQeERdelMddFNnGEynVLIcTDVgg ++L+E ++ G++ ++IH+ ++Q ER +I++ +G+Y V ++T V+G

Query 458 DLLSEAVQKITGLKSISIHSEKSQIERKNILKGLLEGDYEVVVSTGVLG 506

HMM RGIDIPdVNHVINYDMPWNPEqYIQRIGRTgRIG* RG+D+++V++V+N+DMP +++ Y++ + T +

Query 507 RGLDLISVRLVVNFDMPSSMDEYVH-QENTYKST 539

DKFZphfbr2_23b21

group: signal transduction

DKFZphfbr2_23b21.1 encodes a novel 193 amino acid protein which is nearly identical to bovme neurocalcin.

Neurocalcin is a Ca (2+) -binding protein with three putative Ca (2+) -binding domains (EF-hands). In cattle, 6 isoforms are differentially expressed in the central nervous system, retina and adrenal gland. Homology with recoverin indicates involvement in Ca2+ dependent activation of guanylate cyclase.

The new protein can find application in modulating/blocking the guanylate cyclase-pathway . nearly identical to bovme neurocalcin eds complete cDNA

Sequenced by AGOWA

Locus: /map="574.6 cR from top of Chr8 linkage group"

Insert length: 3300 bp

Poly A stretch at pos. 3279, polyadenylation signal at pos. 3249

1 GGGGAGAATC TGGTGGATGC TGGACCTTGC TGCTGCTGCT ACTGCTGTTT

51 CCAGGGGCTG CAGAGCATGG ACTGTTAAAT CTTGCACTTC TTCTGAGTGA

101 GCTGAATTCT TGCCGCCAGG ATGGGGAAAC AGAACAGCAA GCTGCGCCCG

151 GAGGTCATGC AGGACTTGCT GGAAAGCACA GACTTTACAG AGCATGAGAT

201 CCAGGAATGG TATAAAGGCT TCTTGAGAGA CTGCCCCAGT GGACATTTGT

251 CAATGGAAGA GTTTAAGAAA ATATATGGGA ACTTTTTCCC TTATGGGGAT

301 GCTTCCAAAT TTGCAGAGCA TGTCTTCCGC ACCTTCGATG CAAATGGAGA

351 TGGGACAATA GACTTTAGAG AATTCATCAT CGCCTTGAGT GTAACTTCGA

401 GGGGGAAGCT GGAGCAGAAG CTGAAATGGG CCTTCAGCAT GTACGACCTG

451 GACGGAAATG GCTATATCAG CAAGGCAGAG ATGCTAGTGA TCGTGCAGGC

501 AATCTATAAG ATGGTTTCCT CTGTAATGAA AATGCCTGAA GATGAGTCAA

551 CCCCAGAGAA AAGAACAGAA AAGATCTTCC GCCAGATGGA CACCAATAGA

601 GACGGAAAAC TCTCCCTGGA AGAGTTCATC CGAGGAGCCA AAAGCGACCC

651 GTCCATTGTG CGCCTCCTGC AGTGCGACCC GAGCAGTGCC GGCCAGTTCT

701 GAGCCCTGCG CCCACCAATC GAATTGTAGA GCTGCTTGTG TTCCCTTTTG

751 ATTCTTCTTT TTAACAATTT TTTTTTTTTT TTGCCAAACA ATATCAATGG

801 TGATGCCGTC CCCTGTGCGG TCTGATGCGC CTTCCTCCGT GACGCCTTCA

851 GCCTCTTTTG TCGTGGATGC TTCGTGGGAA TGCCCAGAGC CCCAGTGTGC

901 TTGTGGAGAG CATGGACAGA CTTCGTGGTG TTCATTGTTT GATGATTTTT

951 AATCGTTACT ATTATTTCTT TTTATTCTAA TGTCTCTGTT CTAAAACGTA

1001 AGACTCGGGG GTTGGGGCAA AAGAAGGGAA ACCCATCCAG TCCTGTGATT

1051 CTATTGCAAG CTTCAAGGGG CTTTTGTTTG AAAGACAAAA CTCCCCACCT

1101 GGGTCTGTTG TCACACGTGC CGTAGGGGTG ATGGATGGCA CCGGATGCTG

1151 GATTCCCCAA GAACAAGTTA CCCTCTGGGG TGAGGCTATT CCAGCGAGCT

1201 GGGACATTTC CCCATGGGGG CCCACTCCCC TCTCTTCCCC AGCAGGCTGT

1251 AGTTTCTAAG CTGTGAACAT TTCAAGATAA ATTAACAGAG GAGAGGAAAA

1301 AGATGGCTCA GCTATTTTTT CACAGGTTTA CACTAGTTGA GCTAATATGC

1351 GTGTCTTTGG AAATTAAACA CAAATGGTAA CATATTCCAA AACCAGACCC

1401 ATCTTGTTGC CTATTGTGAT AAAATAAAAA GACGGCTGTA TATAACATAT

1451 TGGGTAATGC AGACCAAATT AAGTGTTTTG CCTTGTTTAA ATGAAATGCA

1501 TGTTTAGTGA GCACTAATAC AATCTTATTC CAGAAGACTG TTTTTAGTAG

1551 CTTATTGTGA AGTAAGACAA CTATAATGAA TGTCTGTCTT GTTTGGAAGT

1601 CATATCTGTC TTTGCACAAA TGTACCAATC GACAAGTATA TTTTATATAT

1651 TCCATAAAAA TACAAAGTAA CCCTGACTAG GGCCCAACTT TAATTTTGAA

1701 TGCATTTCCA GAGTGGCCAT GCCTAGAGGG CAGATGCAGA GCAGGTGGTA

1751 GTGGGACAGG ACAATTGGAG CACAGGAATG TTAACATGTA TGACAGGGGA

1801 CCAGTAGGGT GGTTTCCCTC TCAGGCCCAG CAGCCCATTG ACAGCATTAG

1851 ACTGGCGGCA TGGTGCTTTT CTGAGCAGAT CAATACTCTG CAGACTCGAA

1901 AAAACATCAC ATACATTCTT GGAACTTCCC AGTGGTTTAA TCTATGTGCA

1951 TGGTTAGGGA GCCAGGCCTG GAATATTCAG TTTCCCTGCC CCTGTTAAAG

2001 AATCAGAGGT TGGGCAGTCA TCAAATTCAT CATAAAGACA TGGGCAAGTG

2051 TGTCTGTGGT TTCCAAGGCC CCCCTATGGA GAATCCAAAA GTATTTTCCA

2101 TTGCCGTGCT CTTTGAATGC AGACTTCTAT TTCCAGAAGT GACAGCACAA

2151 GTCTGAGTTG CTGTTTGGTC TGGTGACCTC AGACACACTA ATTTGAATTG

2201 AAAGCTAAGA GTAAAAATTT GCTGGTTACA GGCGAGTCAT ACTCTTGCAA

2251 GTAGTTAGCA AAGGGAGGCC CAAATTCTCA AGGTTGTTGA TGGGGAACTT

2301 GCCACTAAGA GAAGGCAGAG AGGTCCCTAG TGGGTATATT TGCTGCCAAG

2351 CCACTTGCCA AAGAAGAGGA ACCACAGAAA GAGAGACATC ATGACCAGGA

2401 GAAAAATGTG ACTAGACATG CTAACCTCCA GGTTTTTATA TATGACTTGA

2451 GTCTGCTGTA ATTGGCAGCA GAAATCCAAA TTTGTATGGT AGACCAAAAA

2501 GAACCAAATC CATAGGGTGA AATTTTGAGA CCTAGACTCT GTAAAAATAA 2551 TCCTAGTCTT CCTCCAGGGG TCAGTTCCTC ACAGTGGTTC TGTACCAAAA 2601 CTTGCCAAAT TCCTCCATGG CCAAGTGTTA AAATCTGTGT TTGGAAAATA 2651 GCGAATTAAC CTAAGACACA GAAGGCAGAC TGGGTGAGGA GACCTAGCAT 2701 GCCCTATTGG CAGTGCTCAG GAGCTGCATC CCACTTTTCC CTGCTCTGAA 2751 TCGAAGTCCT AGTTCCTTCC TTTGATTCTC CTTTGGTAGG TGGAATCAGT 2801 TAATGTTTTG AGAAACCTGC CTGGGCTCTG CCCTTAGTCA TGACATCTCG 2851 CTGAGCCAGA CCCACTCTGT TCCTTGGAAC CTAGAGCTGG AGTGAGGAGT 2901 AGAGGTCTCC GGCTATTCCA GAAAGAAAAG TGAGCCACAT GCAGGCTGAT 2951 GAATGCCGAC ACTTCCAGAA TGTATAGAAA TAGTCCCTGT CCTGGCCTGC 3001 CACTGACCCT GTCTGTATTT TCTCGGAGGT TGTTTTTCTC CTTCTCCTTC 3051 CCAGGAAGGT CTTTGTATGT CGAATCCAGT GCACTCAAGT TTGGCCAAGG 3101 GACTCCACAG CACCCAGAGG ACTGCATGCC TCAAGGTTTA TGTCACTCCT 3151 CTGCTGGGCT GTTCATTGTC ATTGCTGTGT TCAGGGACCT TTGGAAATAA 3201 AACCTGTTCT GTCCCAAATA AAACCAGCCT GTGATGTTCA AGGGACTGGA 3251 ATAAAGTGGC TTACGACCTG AAGGATTCTA AAAAAAAAAA AAAAAAAAAA

BLAST Results

Entry HS431350 from database EMBL: human STS I-15914. Score = 1308, P = 3.1e-53, identities = 276/285

Entry HSG19929 from database EMBL: human STS A002C26. Score = 926, P = 1.5e-35, identities = 186/187

Entry AF052142 from database EMBL:

Homo sapiens clone 24665 mRNA sequence.

Score = 7378, P = O.Oe+00, identities = 1482/1487

3' UTR

Medlme entries

93247712:

Neurocalcin family: a novel calcium-bindmg protein abundant in bovine central nervous system.

94045365:

Distinct regional localization of neurocalcin, a Ca (2+) -binding protein, m the bovine adrenal gland.

96407688:

Crystallization and preliminary X-ray crystallographic studies of recombinant bovine neurocalcin delta.

96066284:

Distribution pattern of three neural calcium-binding proteins (NCS-1,

VILIP and recoverin) in chicken, bovine and rat retina.

Peptide information for frame 1

ORF from 121 bp to 699 bp; peptide length: 193 Category: strong similarity to known protein Prosite motifs: EF_HAND (73-86) EF_HAND (109-122) EF HAND (157-170)

1 MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK

51 IYGNFFPYGD ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK

101 LKWAFSMYDL DGNGYISKAE MLVIVQAIYK MVSSVMKMPE DESTPEKRTE

151 KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA GQF

BLASTP hits

Entry JH0616 from database PIR: neurocalcin (clone pCalN) - bovine Score = 1001, P = 5.2e-101, identities = 192/193, positives = 192/193

Entry GGU91630_1 from database TREMBL: product: "neurocalcin"; Gallus gallus neurocalcin mRNA, complete eds.

Score = 998, P = l.le-100, identities = 191/193, positives = 192/193

Entry NECD_BOVIN from database SWIΞSPROT:

NEUROCALCIN DELTA.

Score = 996, P = 1.8e-100, identities = 191/192, positives = 191/192

Entry S47565 from database PIR:

BDR-1 protein - human

Score = 934, P = 6.6e-94, identities = 174/193, positives = 187/193

Entry 150676 from database PIR: gene Rem-1 protein - chicken >TREMBL:GGREM1_1 gene: "Rem-1"; G. gallus rem-1 mRNA

Score = 933, P = 8.4e-94, identities = 174/193, positives = 186/193

Alert BLASTP hits for DKFZphfbr2_23b21, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_23b21, frame 1

Report for DKFZphfbr2_23b21.1

[LENGTH] 193

[MW] 22215.30

[pi] 5.35

[HOMOL] PIR:JH0616 neurocalcin (clone pCalN) - bovme le-109

[FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YDR373w] 3e-54

[FUNCAT] 30.03 organization of cytoplasm [Ξ. cerevisiae, YKL190w] 2e-18

[FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins

[S. cerevisiae, YKL190w] 2e-18

[FUNCAT] 03.01 cell growth [S. cerevisiae YKL190w] 2e-18

[FUNCAT] 13.04 homeostasis of other ions [S. cerevisiae, YKL190w] 2e-18

[FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YKL190w] 2e-18

[FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YBR109C] 0.001

[FUNCAT] 08.19 cellular import [S. cerevisiae, YBR109c] 0.001

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YBR109C] 0.001

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YBR109c]

0.001

[ FUNCAT] 10.02.99 other morphogenetic activities [S. cerevisiae, YBR109c] 0.001

[FUNCAT] 30.05 organization of centrosome [S. cerevisiae, YBR109c] 0.001

[BLOCKS] BL00018

[SCOP] dlrec 1.34 .1.5.18 Recoverin [bovine (Bos taurus) 8e-55

[SCOP] dl sa 1.34.1.5.17 Recoverin [human (Homo sapiens) 5e-58

[SCOP] dltcob_ 1.34.1.5.16 Calcineurin regulatory subunit (B-chain le-06

[SCOP] d2mysc_ 1.34.1.5.15 Myosin Regulatory Chain [chicken (Gallu 2e-29

[SCOP] dlscmc 1.34.1.5.14 Myosin Regulatory Chain [bay scallo 5e-33

[SCOP] d2mysb _ 1.._34..1.5.13 Myosin Essential Chain [chicken (Gallu 4e-26

[SCOP] dlscmb 1.34.1.5.12 Myosin Essential Chain [bay scallo 6e-27

[SCOP] dlclm_ 1.34.1.5.11 Calmodulin [Paramecium tetraurelia le-15

[SCOP] d4cln 1.34.1 5.10 Calmodulin [Drosophila melanogaster 2e-16

[SCOP] dlcfc 1.34.1.5.9 Calmodulin [African frog (Xenopus laevis) 2e-16

[SCOP] dlahr ' 11.34.1.5.8 Calmodulin [chicken gallus gallus 4e-16

[SCOP] d3cln 34.1.5 Calmodulin [rat (Rattus rattus) 2e-16

[SCOP] dltrcb_ 34.1.5 6 Calmodulin [bovme (Bos taurus) 8e-08

[SCOP] dlcll 34.1.5.5 Calmodulin [human (Homo sapiens) 2e-16

[SCOP] dlrtpl_ 34.1.4.5 Parvalbumin [rat (Rattus rattus) 8e-06

[SCOP] d5tnc 34.1.5.2 Troponin C [turkey (Meleagris gallopavo) 3e-13

[SCOP] dlpvaa_ 34.1...4,.3 Parvalbumin [pike (Esox lucius) 6e-06

[SCOP] dltnp_x. 34.1.5.1 Troponin C [chicken (Gallus gallus) 9e-ll

[EC] 2.7.1.107 Diacylglycerol kinase 2e-08

[PIRKW] blocked amino end le-100

[PIRKW] phosphotransferase 2e-08

[PIRKW] duplication 4e-17

[PIRKW] tandem repeat 7e-06

[PIRKW] heterodimer 4e-17

[PIRKW] heart 6e-09

[PIRKW] zinc 2e-08

[PIRKW] serine/threonine-specific protein kinase le-06

[PIRKW] muscle contraction le-08

[PIRKW] acetylated amino end 4e-09

[PIRKW] ATP 2e-08

[PIRKW] skeletal muscle 6e-09 [PIRKW] signal transduction le-91

[PIRKW] protein kinase 2e-08

[PIRKW] calcium binding le-100

[PIRKW] alternative splicing 2e-13

[PIRKW] methylated amino acid le-09

[PIRKW] thin filaments le-08

[PIRKW] lipoprotein le-101

[PIRKW] cardiac muscle 6e-09

[PIRKW] muscle 6e-09

[PIRKW] myristylation le-100

[PIRKW] EF hand le-101

[PIRKW] retina 2e-51

[SUPFAM] calcium-dependent protein kinase 2e-08

[SUPFAM] unassigned calmodulin-related proteins 8e-41

[SUPFAM] spec-related protein LpSl 7e-06

[SUPFAM] calmodulin repeat homology le-101

[SUPFAM] human diacylglycerol kinase 2e-08

[SUPFAM] protein kinase C zinc-binding repeat homology 2e-0£

[SUPFAM] protein kinase homology 2e-08

[SUPFAM] calmodulin le-101

[PROSITE] EF_HAND 3

[PROSITE] CK2_PHOSPHO_SITE 7

[PROSITE] PKC_PHOSPHO_SITE 3

[PFAM] EF hand

[KW] All_Alpha

[KW] 3D

SEQ MGKQNSKLRPEVMQDLLESTDFTEHEIQEWYKGFLRDCPSGHLSMEEFKKIYGNFFPYGD lrec- HHHHHHHHHTTTTCCCHHHHHHHHHHHHHHTTTTEEEHHHHHHHHHHHTTTTC

SEQ ASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGKLEQKLKWAFSMYDLDGNGYISKAE lrec- HHHHHHHHHHHH CEEEHHHHHHHHHHHHCCCGGGHHHHHHHHHTTTTCCCEEHHH

SEQ MLVIVQAIYKMVSSVMKMPEDESTPEKRTEKIFRQMDTNRDGKLSLEEFIRGAKSDPSIV lrec- HHHHHHHHHHCCTTGGGCTTTTTCHHHHHHHHHHHHCCTTTTEECHHHHHHHHHHCHHHH

SEQ RLLQCDPSSAGQF lrec- HHHCCCH

Prosite for DKFZphfbr2_23b21.1

PS00005 92->95 PKC PHOSPHO SITE PDOC00005

PS00005 149->152 PKC PHOSPHO^{" "}SITE PDOC00005

PS00005 158->161 PKC PHOSPHO^{" "}SITE PDOC00005

PS00006 23->27 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 44->48 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 106->110 CK2 PHOSPHO^{" '}SITE PDOC00006

PS00006 117->121 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 143->147 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 158->162 CK2 PHOSPHO- ^"SITE PDOC00006

PS00006 165->169 CK2 PHOSPHO- ^"SITE PDOC00006

PS00018 73->86 EF HAND PDOC00018

PS00018 109->122 EF HAND PDOC00018

PS00018 157->170 EF HAND PDOC00018

Pfam for DKFZphfbr2_23b21.1

HMM_NAME EF hand

HMM *MFrmMDkDGDGyIDFEEFmeMMkem*

+FR +D +GDG+IDF EF+ +++ Query 68 VFRTFDANGDGTIDFREFIIALSVT 92

30.75 100 128 1 29 dkfzphfbr2_23b21.1 nearly identical to bovine neurocalcin

Alignment to HMM consensus: Query *EIqEMFrmMDkDGDGyIDFEEFmeMMkem*

++++F+M+D DG+GYI++ E++++++++ dkfzphfbr2 100 KLKWAFSMYDLDGNGYISKAEMLVIVQAI 128

Query 176 1 29 dkfzphfbr2_23b21.1 nearly identical to bovine neurocalcin

Alignment to HMM consensus: HMM *EIqEMFrmMDkDGDGyIDFEEFmeMMkem*

+++FR MD+++DG+++ EEF++ K+ Query 148 RTEKIFRQMDTNRDGKLSLEEFIRGAKSD 176 DKFZphfbr2_23f2

group: brain derived

DKFZphfbr2_23f2 encodes a novel 182 amino acid protein with weak similarity to S. pombe Vps29p.

No informative BLAST results; no predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . similarity to Vps29p complete cDNA, complete eds, EST hits

S. cerevisiae and S. pombe Vps29p are involved in vacuolar protein sorting part of the cDNA is encoded by HSAC2350, splice pattern 4 exons

Sequenced by AGOWA

Locus: /map="12q24"

Insert length: 1016 bp

Poly A stretch at pos. 996, polyadenylation signal at pos. 974

1 GAATGGGGAG GAGCCAGAGG AAGAGGGCGG CGACGGTGGT GGTGACTGAG

51 CGGAGCCCGG TGACAGGATG TTGGTGTTGG TATTAGGAGA TCTGCACATC

101 CCACACCGGT GCAACAGTTT GCCAGCTAAA TTCAAAAAAC TCCTGGTGCC

151 AGGAAAAATT CAGCACATTC TCTGCACAGG AAACCTTTGC ACCAAAGAGA

201 GTTATGACTA CCTCAAGACT CTGGCTGGTG ATGTTCATAT TGTGAGAGGA

251 GACTTCGATG AGAATCTGAA TTATCCAGAA CAGAAAGTTG TGACTGTTGG

301 ACAGTTCAAA ATTGGTCTGA TCCATGGACA TCAAGTTATT CCATGGGGAG

351 ATATGGCCAG CTTAGCCCTG TTGCAGAGGC AATTTGATGT GGACATTCTT

401 ATCTCGGGAC ACACACACAA ATCTGAAGCA TTTGAGCATG AAAATAAATT

451 CTACATTAAT CCAGGTTCTG CCACTGGGGC ATATAATGCC TTGGAAACAA

501 ACATTATTCC ATCATTTGTG TTGATGGATA TCCAGGCTTC TACAGTGGTC

551 ACCTATGTGT ATCAGCTAAT TGGAGATGAT GTGAAAGTAG AACGAATCGA

601 ATACAAAAAA CCTTAAAGCC AGGCCTGTCT TGATGATTTT TGGTTTTTTT

651 TCATTGTCCT GTTGAAATCA AGTAATTAAA CATTTAAGAG CCACAAAATT

701 GTATCACTTT TATAATATTT TGCAGTAAAA TATAATACCA TCTTCTCTGT

751 TAATACATAA TTGCTCCAAG CTTCCTGTAA ACTATAAGAA TATATTTAGT

801 TTACAGTATA TGGATTCTAT GAAAAAATGT CCACAACACA GTAATTGGTC

851 ACTTGTTAAG AAAAATTTAT CCTTGTAAGT ATCTTCAAAG TTGATATTTG

901 GAACTTTATT CCAAAAGTAG TGCATGTGGA GAAAGAATCT AGACTTTCTT

951 GTATACATTT TTCTCTTCTC CAGTAATAAA CAATTACCTT TCATTGAAAA

1001 AAAAAAAAAA AAAAAA

BLAST Results

Entry HSAC2350 from database EMBLNEW:

Homo sapiens 12q24 PAC P424M6 Length = 167,217

Medlme entries

No Medline entry

Peptide information for frame 2

ORF from 68 bp to 613 bp; peptide length: 182 Category: similarity to known protein Prosite motifs: RGD (60-63)

1 MLVLVLGDLH IPHRCNSLPA KFKKLLVPGK IQHILCTGNL CTKESYDYLK

51 TLAGDVHIVR GDFDENLNYP EQKVVTVGQF KIGLIHGHQV IPWGDMASLA

101 LLQRQFDVDI LISGHTHKSE AFEHENKFYI NPGSATGAYN ALETNIIPSF 151 VLMDIQASTV VTYVYQLIGD DVKVERIEYK KP

BLASTP hits

Entry CEZK1128_6 from database TREMBL:

"ZK1128.1"; Caenorhabditis elegans cosmid ZK1128

Length = 523

Score = 400 (140.8 bits), Expect = 2.3e-37, P = 2.3e-37

Identities = 81/150 (54%), Positives = 106/150 (70%)

Entry S46793 from database PIR: hypothetical protein YHR012c - yeast (Saccharomyces cerevisiae)

Length = 282

Score = 180 (63.4 bits), Expect = 3.7e-37, Sum P(3) = 3.7e-37

Identities = 35/71 (49%), Positives = 44/71 (61%)

Entry AB011824_1 from database TREMBL:

"Vps29"; Schizosaccharomyces pombe mRNA for Vps29, partial eds. Schizosaccharomyces pombe (fission yeast)

Length = 176

Score = 189 (66.5 bits), Expect = 2.7e-27, Sum P(2) = 2.7e-27

Identities = 33/72 (45%), Positives = 50/72 (69%)

Alert BLASTP hits for DKFZphfbr2_23f2, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_23f2, frame 2

Report for DKFZphfbr2_23f2.2

[LENGTH] 182 [MW] 20445.84 [pi] 6.29 [HOMOL] TREMBL:CEZK1128_6 gene: "ZK1128.8"; Caenorhabditis elegans cosmid ZK1128 2e-51

[FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YHR012w] le-27

[FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YHR012w] le-27

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YHR012w] le-27

[FUNCAT] 30.08 organization of golgi [S. cerevisiae, YHR012w] le-27

[FUNCAT] 09.25 vacuolar and lysosomal biogenesis [S. cerevisiae, YHR012w] le-27

[FUNCAT] r general function prediction [M. jannaschn, MJ0623] le-16

[BLOCKS] BL01269D

[BLOCKS] BL01269A

[PROSITE] RGD 1

[PROSITE] MYRISTYL 4

[PROSITE] PKC_PHOSPHO_SITE 1

[KW] Alpha_Beta

SEQ MLVLVLGDLHIPHRCNSLPAKFKKLLVPGKIQHILCTGNLCTKEΞYDYLKTLAGDVHIVR PRD ccceeecccccccccccchhhhhhhhhhcceeeeeecccccchhhhhhhhhhhhceeeee

SEQ GDFDENLNYPEQKVVTVGQFKIGLIHGHQVIPWGDMASLALLQRQFDVDILISGHTHKSE PRD cccccccccccceeeeeccceeeeecccccccccchhhhhhhhhhhcceeeeeccccccc

SEQ AFEHENKFYINPGSATGAYNALETNIIPSFVLMDIQASTVVTYVYQLIGDDVKVERIEYK PRD ccccccccccccccccccccccccccccceeeeeccccceeeeeeeecccceeeeeeeec

SEQ KP

PRD cc

Prosite for DKFZphfbr2_23f2.2

PS00005 116->119 PKC_PHOSPHO_ΞITE PDOC00005

PS00008 38->44 MYRISTYL PDOC00008

PS00008 83->89 MYRISTYL PDOC00008

PS00008 133->139 MYRISTYL PDOC00008

PS00008 137->143 MYRISTYL PDOC00008

PS00016 60->63 RGD PDOC00016

(No Pfam data available for DKFZphfbr2_23f2.2) DKFZphfbr2_23124

group: intracellular transport and trafficking

DKFZphfbr2_23124.2 encodes a novel 348 amino acid protein with similarity to human glycoprotein gp36b and canine VIP36 glycoprotein.

The vesicular protein VIP36 (36 kDa vesicular integral membrane protein) shows homology to leguminous plant lectins . The protein is localized to the Golgi apparatus, endosomal and vesicular structures and the plasma membrane. VIP36 binds to sugar residues of glycosphmgolipids and/or glycosylphosphatidyl-inositol anchors and might provide a l nk between the extracellular/lummal face of glycolipid rafts and the cytoplasmic protein segregation machinery. Gp36 is located within the endoplasmatic reticulum. For the novel protein, a lectin character is predicted. Due to the intracellular localisation of the homolog proteins, it should be involved in intracellular transport and trafficking.

The new protein can find application in modulating/blocking intracellular transport and trafficking. strong similarity to human GP36b glycoprotein complete cDNA, complete eds, EST hits potential start at Bp 29 matches kozak consensua ANNatgG similarity to lectins,

Sequenced by AGOWA

Locus: /map="2"

Insert length: 2416 bp

Poly A stretch at pos. 2394, no polyadenylation signal found

1 GGGGGATGAA GGGTCGTTGG TGGGAAAGAT GGCGGCGACT CTGGGACCCC

51 TTGGGTCGTG GCAGCAGTGG CGGCGATGTT TGTCGGCTCG GGATGGGTCC

101 AGGATGTTAC TCCTTCTTCT TTTGTTGGGG TCTGGGCAGG GGCCACAGCA

151 AGTCGGGGCG GGTCAAACGT TCGAGTACTT GAAACGGGAG CACTCGCTGT

201 CGAAGCCCTA CCAGGGTGTG GGCACAGGCA GTTCCTCACT GTGGAATCTG

251 ATGGGCAATG CCATGGTGAT GACCCAGTAT ATCCGCCTTA CCCCAGATAT

301 GCAAAGTAAA CAGGGTGCCT TGTGGAACCG GGTGCCATGT TTCCTGAGAG

351 ACTGGGAGTT GCAGGTGCAC TTCAAAATCC ATGGACAAGG AAAGAAGAAT

401 CTGCATGGGG ATGGCTTGGC AATCTGGTAC ACAAAGGATC GGATGCAGCC

451 AGGGCCTGTG TTTGGAAACA TGGACAAATT TGTGGGGCTG GGAGTATTTG

501 TAGACACCTA CCCCAATGAG GAGAAGCAGC AAGAGCGGGT ATTCCCCTAC

551 ATCTCAGCCA TGGTGAACAA CGGCTCCCTC AGCTATGATC ATGAGCGGGA

601 TGGGCGGCCT ACAGAGCTGG GAGGCTGCAC AGCCATTGTC CGCAATCTTC

651 ATTACGACAC CTTCCTGGTG ATTCGCTACG TCAAGAGGCA TTTGACGATA

701 ATGATGGATA TTGATGGCAA GCATGAGTGG AGGGACTGCA TTGAAGTGCC

751 CGGAGTCCGC CTGCCCCGCG GCTACTACTT CGGCACCTCC TCCATCACTG

801 GGGATCTCTC AGATAATCAT GATGTCATTT CCTTGAAGTT GTTTGAACTG

851 ACAGTGGAGA GAACCCCAGA AGAGGAAAAG CTCCATCGAG ATGTGTTCTT

901 GCCCTCAGTG GACAATATGA AGCTGCCTGA GATGACAGCT CCACTGCCGC

951 CCCTGAGTGG CCTGGCCCTC TTCCTCATCG TCTTTTTCTC CCTGGTGTTT

1001 TCTGTATTTG CCATAGTCAT TGGTATCATA CTCTACAACA AATGGCAGGA

1051 ACAGAGCCGA AAGCGCTTCT ACTGAGCCCT CCTGCTGCCA CCACTTTTGT

1101 GACTGTCACC CATGAGGTAT GGAAGGAGCG GGCACTGGCC TGAGCATGCA

1151 GCCTGGAGAG TGTTCTTGTC TCTAGCAGCT GGTTGGGGAC TATATTCTGT

1201 CACTGGAGTT TTGAATGCAG GGACCCCGCA TTCCCATGGT TGTGCATGGG

1251 GACATCTAAC TCTGGTCTGG GAAGCCACCC ACCCCAGGGC AATGCTGCTG

1301 TGATGTGCCT TTCCCTGCAG TCCTTCCATG TGGGAGCAGA GGTGTGAAGA

1351 GAATTTACGT GGTTGTGATG CCAAAATCAC GGAACAGAAT TTCATAGCCC

1401 AGGCTGCCGT GTTGTTTGAC TCAGAAGGCC CTTCTACTTC AGTTTTGAAT

1451 CCACAAAGAA TTAAAAACTG GTAACACCAC AGGCTTTCTG ACCATCCATT

1501 CGTTGGGTTT TGCATTTGAC CCAACCCTCT GCCTACCTGA GGAGCTTTCT

1551 TTGGAAACCA GGATGGAAAC TTCTTCCCTG CCTTACCTTC CTTTCACTCC

1601 ATTCATTGTC CTCTCTGTGT GCAACCTGAG CTGGGAAAGG CATTTGGATG

1651 CCTCTCTGTT GGGGCCTGGG GCTGCAGAAC ACACCTGCGT TTCGCTGGCC

1701 TTCATTAGGT GGCCCTAGGG AGATGGCTTT CTGCTTTGGA TCACTGTTCC

1751 CTAGCATGGG TCTTGGGTCT ATTGGCATGT CCATGGCCTT CCCAATCAAG

1801 TCTCTTCAGG CCCTCAGTGA AGTTTGGCTA AAGGTTGGTG TAAAAATCAA

1851 GAGAAGCCTG GAAGACACCA TGGATGCCAT GGATTAGCTG TGCAACTGAC

1901 CAGCTCCAGG TTTGATCAAA CCAAAAGCAA CATTTGTCAT GTGGTCTGAC

1951 CATGTGGAGA TGTTTCTGGA CTTGCTAGAG CCTGCTTAGC TGCATGTTTT

2001 GTAGTTACGA TTTTTGGAAT CCCTCTTTGA GTGCTGAAAG TGTAAGGAAG

2051 CTTTCTTCTT ACACCTTGGG CTTGGATATT GCCCAGAGAA GAAATTTGGC

2101 TTTTTTTTCT TAATGGACAA GGGACAGTTG CTGTTCTCAT GTTCCAAGTC

2151 TGAGAGCAAC AGACCCTCAT CATCTGTGCC TGGAAGAGTT CACTGTCATT

2201 GAGCAGCACA GCCTGAGTGC TGGCCTCTGT CAACCCTTAT TCCACTGCCT 2251 TATTTGACAA GGGGTTACAT GCTGCTCACC TTACTGCCCT GGGATTAAAT

2301 CAGTTACAGG CCAGAGTCTC CTTGGAGGGC CTGGAACTCT GAGTCCTCCT

2351 ATGAACCTCT GTAGCCTAAA TGAAATTCTT AAAATCACCG ATGGAACCAA

2401 AAAAAAAAAA AAAAAA

BLAST Results

Entry HS622145 from database EMBL: human STS WI-6746. Score = 1079, P = 5.1e-43, identities = 219/223

Entry G42541 from database EMBLNEW:

SHGC-58649 Human Homo sapiens STS genomic, sequence tagged site.

Score = 1091, P = 1.7e-43, identities = 219/220

Medlme entries

94265253:

A putative novel class of animal lectins m the secretory pathway homologous to leguminous lectins .

94208543:

VIP36, a novel component of glycolipid rafts and exoeytic carrier vesicles in epithelial cells.

Peptide information for frame 2

ORF from 29 bp to 1072 bp; peptide length: 348 Category: strong similarity to known protein

1 MAATLGPLGS WQQWRRCLSA RDGSRMLLLL LLLGSGQGPQ QVGAGQTFEY

51 LKREHSLSKP YQGVGTGSSS LWNLMGNAMV MTQYIRLTPD MQSKQGALWN

101 RVPCFLRDWE LQVHFKIHGQ GKKNLHGDGL AIWYTKDRMQ PGPVFGNMDK

151 FVGLGVFVDT YPNEEKQQER VFPYISAMVN NGSLSYDHER DGRPTELGGC

201 TAIVRNLHYD TFLVIRYVKR HLTIMMDIDG KHEWRDCIEV PGVRLPRGYY

251 FGTSSITGDL SDNHDVISLK LFELTVERTP EEEKLHRDVF LPSVDNMKLP

301 EMTAPLPPLS GLALFLIVFF SLVFSVFAIV IGIILYNKWQ EQSRKRFY

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_23124, frame 2

PIR:G01447 GP36b glycoprotein - human, N = 1, Score = 1001, P = 5.9e-101

SWIΞSPROT:VP36_CANFA VESICULAR INTEGRAL-MEMBRANE PROTEIN VIP36 PRECURSOR (VIP36)., N = 1, Score = 990, P = 8.6e-100

TREMB :CET04G9_2 gene: "T04G9.3"; Caenorhabditis elegans cosmid T04G9., N = 1, Score = 614, P = 6e-60

PIR:S42626 ER-golgi intermediate compartment protein - human, N = 2, Score = 397, P = le-42

>PIR:G01447 GP36b glycoprotein - human Length = 356

HSPs:

Score = 1001 (150.2 bits), Expect = 5.9e-101, P = 5.9e-101 Identities = 197/356 (55%), Positives = 256/356 (71%)

Query: 1 MAATLGPLGSWQQWRRCLSARDG SRMLLLLLLLGSGQGPQQVGAGQTFEYLK 52

MAA G + W RRCL R G + L LLLLLGS + G + E+LK Sbjct: 1 MAAE-GWIWRWGWGRRCLG-RPGLLGPGPGPTTPLFLLLLLGΞVTA--DITDGNS-EHLK 55 Query: 53 REHΞLSKPYQGVGTGSSSLWNLMGNAMVMTQYIRLTPDMQSKQGALWNRVPCFLRDWELQ 112

REHSL KPYQGVG+ S LW+ G+ M+ +QY+RLTPD +SK+G++WN PCFL+DWE+ Sbjct: 56 REHSLIKPYQGVGSSSMPLWDFQGSTMLTSQYVRLTPDERSKEGSIWNHQPCFLKDWEMH 115

Query: 113 VHFKIHGQGKKNLHGDGLAIWYTKDRMQPGPVFGNMDKFVGLGVFVDTYPNEEKQQERVF 172

VHFK+HG GKKNLHGDG+A+WYT+DR+ PGPVFG+ D F GL +F+DTYPN+E ERVF Sbjct: 116 VHFKVHGTGKKNLHGDGIALWYTRDRLVPGPVFGSKDNFHGLAIFLDTYPNDETT-ERVF 174

Query: 173 PYISAMVNNGSLSYDHERDGRPTELGGCTAIVRNLHYDTFLVIRYVKRHLTIMMDIDGKH 232

PYIS MVNNGSLSYDH +DGR TEL GCTA RN +DTFL +RY + LT+M D++ K+ Sbjct: 175 PYISVMVNNGSLSYDHSKDGRWTELAGCTADFRNRDHDTFLAVRYSRGRLTVMTDLEDKN 234

Query: 233 EWRDCIEVPGVRLPRGYYFGTSSITGDLSDNHDVISLKLFELTVERTPEEEKLHRDVFLP 292

EW++CI++ GVRLP GYYFG S+ TGDLSDNHD+IS+KLF+L VE TP+EE + P Sbjct: 235 EWKNCIDITGVRLPTGYYFGASAGTGDLSDNHDIISMKLFQLMVEHTPDEESIDWTKIEP 294

Query: 293 SVDNMKLPEMTAPLP PLSGLALFLIVFFSLVFSVFAIVIGIILYNKWQEQSRK 345

SV+ +K P+ P PL+G +FL++ +L+ V V+G +++ K QE++ K Sbjct: 295 SVNFLKSPKDNVDDPTGNFRΞGPLTGWRVFLLLLCALLGIVVCAVVGAVVFQKRQERN-K 353

Query: 346 RFY 348

RFY Sbjct: 354 RFY 356

Pedant information for DKFZphfbr2_23124, frame 2

Report for DKFZphfbr2_23124.2

[LENGTH] 348

[MW] 39711.10

[pi] 8.55

[HOMOL] PIR:G01447 GP₃6b glycoprotein human le-101

[PIRKW] lectin 2e-37

[PIRKW] transmembrane protein 2e-37

[PIRKW] endoplasmic reticulum 2e-37

[PIRKW] Golgi apparatus 2e-37

[PROSITE] AMIDATION 1

[PROSITE] MYRISTYL 5

[PROSITE] CK2_PHOSPHO_SITE 2

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE 3

[PROSITE] ASN_GLYCOSYLATION 1

[KW] Alpha_Beta

[KW] SIGNAL_PEPTIDE 39

[KW] LOW COMPLEXITY 7.76 %

SEQ MAATLGPLGSWQQWRRCLSARDGΞRMLLLLLLLGSGQGPQQVGAGQTFEYLKREHSLSKP SEG xxxxxxx PRD ccccccccccccccccccccccchhhhhhhhhhhcccccccccccchhhhhhhhhhhccc

SEQ YQGVGTGSSSLWNLMGNAMVMTQYIRLTPDMQSKQGALWNRVPCFLRDWELQVHFKIHGQ SEG PRD cccccccccceeecccccccccceeeeccchhhhhcccccccccchhhhhhhheeeeecc

SEQ GKKNLHGDGLAIWYTKDRMQPGPVFGNMDKFVGLGVFVDTYPNEEKQQERVFPYISAMVN SEG PRD ccccccccceeeeeecccccccccccccccccceeeeeecccccccccccccceeeeeec

SEQ NGSLSYDHERDGRPTELGGCTAIVRNLHYDTFLVIRYVKRHLTIMMDIDGKHEWRDCIEV SEG PRD ccccccccccccccccccccccccccccccceeeehhhhhhheeeeeccccccccccccc

SEQ PGVRLPRGYYFGTSSITGDLSDNHDVISLKLFELTVERTPEEEKLHRDVFLPΞVDNMKLP SEG PRD cccccccccccccccccccccccchhhhhhhhhhhhhccccccccccccccccccccccc

SEQ EMTAPLPPLSGLALFLIVFFSLVFSVFAIVIGIILYNKWQEQSRKRFY SEG xxxxxxxxxxxxxxxxxxxx

PRD cccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccc

Prosite for DKFZphfbr2_23124.2

PS00001 181->185 ASN_GLYCOSYLATION PDOC00001 PS00002 35->39 GLYCOSAMINOGLYCAN PDOC00002 PS00005 19->22 PKC PHOSPHO SITE PDOC00005 PS00005 268->271 PKC_PHOSPHO_SITE PDOC00005 PS00005 343->346 PKC_PHOSPHO_SITE PDOC00005 PS00006 19->23 CK2_PHOSPHO_SITE PDOC00006 PS00006 279->283 CK2_PHOSPHO_SITE PDOC00006 PS00008 43->49 MYRISTYL PDOC00008 PS00008 63->69 MYRISTYL PDOC00008 PΞ00008 65->71 MYRISTYL PDOC00008 PS00008 96->102 MYRISTYL PDOC00008 PS00008 198->204 MYRISTYL PDOC00008 PS00009 120->124 AMIDATION PDOC00009

(No Pfam data available for DKFZphfbr2_23124.2)

DKFZphfbr2_23nl6

group: signal transduction

DKFZphfbr2_23nl6.1 encodes a novel 292 ammo acid protein with weak similarity to putative phosphatιdylιnosιtol-4-phosphate 5-kmase of Arabidopsis thaliana.

The novel proteins contains a WW domain which has been originally described as a short conserved region in a number of unrelated proteins, among them dystrophin, the gene responsible for Duchenne muscular dystrophy. The domain, which spans about 35 residues, is repeated up to 4 times m some proteins. It has been shown to bind proteins with particular proline-motifs, [AP] -P-P- [AP] -Y, and thus resembles somewhat SH3 domains. This domain is frequently associated with other domains typical for proteins in signal transduction processes. Examples of proteins containing the WW domain are Dystrophin, Utrophin, vertebrate YAP protein (binds the SH3 domain of the Yes oncoprotein) , murine NEDD-4 (embryonic development and differentiation of the central nervous system) , IQGAP (human GTPase activating protein acting on ras) . Therefore the new protein should be involved in intracellular signal transduction.

The new protein can find application in modulating/blocking intracellular signal transduction pathways . similarity to putative phosphatιdylιnosιtol-4-phosphate 5-kιnase complete cDNA, complete eds, EST hits

Sequenced by AGOWA

Locus: unknown

Insert length: 2936 bp

Poly A stretch at pos. 2916, polyadenylation signal at pos. 2873

1 GGGGGCGCTC CCGAGAAAGA GTGAGGGCGC GACGCGCACC AACGGTGGAG 51 GGATGTTTCA GCAGCCCCTG AGAAGGAAGA GGAGGAAGCT GAGGGCCCGC

101 TGAGGGCGCA GGACCTGAGG GAGTCCTACA TCCAGCTCGT CCAGGGTGTG

151 CAGGAGTGGC AGGATGGTTG CATGTACCAG GGGGAGTTTG GGTTGAACAT

201 GAAGCTTGGA TATGGCAAAT TCTCTTGGCC CACAGGCGAG TCATACCATG

251 GGCAGTTTTA CCGGGACCAC TGCCATGGCC TGGGTACCTA CATGTGGCCA

301 GATGGCTCCA GTTTCACGGG CACATTTTAC CTCAGCCACC GAGAAGGCTA

351 CGGCACCATG TACATGAAGA CACGGCTTTT CCAGACTCAC TGCCACAACG

401 ACATTGTCAA CCTTCTCCTG GACTGTGGGG CCGACGTGAA CAAGTGCTCA

451 GATGAGGGTC TCACGGCACT CAGCATGTGT TTCCTCCTCC ACTACCCCGC

501 CCAGTCCTTC AAGCCCAATG TTGCTGAACG GACCATACCT GAGCCCCAGG

551 AACCTCCAAA ATTCCCAGTT GTTCCAATCC TTTCATCATC ATTTATGGAC

601 ACAAACCTGG AGTCTCTGTA CTATGAGGTG AACGTGCCTT CCCAGGGTAG

651 CTATGAGCTG AGGCCACCGC CAGCACCACT GCTCCTGCCA CGCGTCTCAG

701 GCAGCCACGA GGGCGGCCAC TTCCAGGACA CCGGGCAGTG TGGGGGGTCC

751 ATAGACCACA GGAGCAGCTC TCTGAAGGGG GACTCCCCGT TGGTGAAGGG

801 CAGCCTTGGC CATGTGGAAA GCGGGCTTGA GGACGTGTTG GGAGACACAG

851 ACCGGGGCAG TCTGTGCAGT GCTGAGACGA AATTTGAGTC CAACTTGTGT

901 GTGTGCGACT TCTCCATCGA GCTCTCGCAG GCCATGCTGG AGAGAAGCGC

951 CCAGTCCCAC AGCTTGCTGA AGATGGCCTC GCCCTCACCG TGCACCAGCA 1001 GCTTCGACAA AGGGACCATG CGGAGGATGG CGCTGTCCAT GATCGAGTAG 1051 GTCCTGGCAC CAGCTGGTGG GGGTGGAGGG CCACCATCAG GGCTGAATCC 1101 TATGCTCAGC AGACCCACGT CTCTTCCCTG TGCCAGTGGG AGGCGTTGTG 1151 TCTGGAGATG TGTGTCTGAA TGTGTGAGCA TCCCTGTGTC GGTGGCTCCA 1201 TGCCATGGCC AGCCCTGTGG GGGTGCCACG GTGACGGGCT GTTTTCAGTG 1251 CCACCCCAGC CCTGTGGGGG TGCCACGGTG ACGGGCTGTT TTCAGTACCA 1301 CGCCAGCCCT GCTTTGGCCT TTGGCACTGG CCTGAAGTGT CTCTGTGGGA 1351 GCCTCAGCAG GGGCCACTGT CAGGGGTCCT ATCCTAGCCA TAGTGCACGT 1401 GAGTGACACC TGCCTGGGCA GCTCTCACAC CCCTGCTGTC CACCCTGTCT 1451 ATACCAGTGT GTCTCAAAAT GTGGTCTATG CACCCCCGGG GGTCCAAGAC 1501 CCTTTCAGGG AGTCTGTGGG GTCAAAATGA TTCTCTTGAT AACCCTGAGA 1551 CTCTGTTAGC CTTCTCCTTG TGTTGATGTT GGTGGATGGT ATGAAGACAG 1601 GGCCGTGCAG ACCACCAGCC CCCAGCGTGC AGGGCAGCAG TGCCCGGCCT 1651 GCTTGGGGGC ATGGTATTCC TTCACCACGG TGTGCACTTG CGGGGATGCC 1701 TGTCTCACTG AAGAATGCCT TTGACTAAGC AGAAAAGCAA TGACAAATTG 1751 CATTAAATCT TGCTCCTTGC GTACACACCC CTCGAATATT CTGGGTCGGA 1801 AAACATGGGA AGGACACTGA TGTGTGTCTG CCACAGACCA AGGCACACCG 1851 CTTCCCCGCA AGAAGCGCTT CCCCCAGGGC CAGAGTAGCA ACAGAATGCG 1901 GCATCTTCCC AACCTCCTGC CCCATTTTTG ATTGGAAGAA TGACCACTGG 1951 TATGTGGCTG TTCATTCTCC TGAACACAGC CTGCCACTTT AAGGAAAACA 2001 TATGACACTA TTTGTTGCTG GCGAAATTTA CATTTTCAAG TGAATAGCAG 2051 AATTCTGGAC ACTTGCCACC ACCACCAAAA CCTTCATAGC TTCCCTTAAC 2101 TTTGAGACAT GGGTGTTCAG AGGTTTTTCA CGTGAGATGG CGTTAGCAGC 2151 GCAGTTTTGT GATACTGCCT GAAGACATGC CGACAGTGCC CAGATCTCTT 2201 CTATTGGTGA GCCAGCTTTT CCCACACGGC CAAGTTCTGA TGTTGAACCA

2251 TTGCCAGGTG GGTGAAGATC CATTGACAGT GAGAGGTGGG CCCGTGGGCT

2301 TCAGTGCAGC CAGGCGCAGA AGGCTGGTTC ATGAGTGTCC AGCTCCGCCA

2351 GGTAGCTAGC TCACCACCCC CAGCCTGGGT TCATGTAGTT CAAATAGGAA

2401 GACCACGATG ATCAGAAAGG CTGCTCAAAT ACTCCTTCGT CCAGCCGCGT

2451 ACCTGGGGGA GGCTGAATCT CCACTCACTT CCACCAAGGC TGTGCAGAGC

2501 AGATAGGGGA ATCCAGCAAA GGTGGAAAAC AGTGCCATCC TTCTCCCCAA

2551 CTGGTTTTGT TTTGTAAAAT AACTTTTTGT GACAGTGTTA CTTATTAGTA

2601 ACATGCAGTG GGTTTGTTAT GGTTAACAAG TTGGTGAGCA TTATTGAGAG

2651 GTGAAGCCAG CTGAGCTTCT GGGTTGGGTG GGGACTTGGA GAACTTTTGT

2701 GTCTAGCTAA AGGATTGTAA ATGCACCAAT CAATGCTCAG TGTCTAGCTA

2751 AAGGATTGTA AATGCACCAA TCAGCACTCT GTAAAATTGA CCAATCAGCG

2801 TTCTGTAAAA TGGACCAATC AGTGGTCTGT AAAATGGACC AGTCAGCAGG

2851 ATGTGGGCGG GGCCAAAAAA GGGAATAAAA GCTGGCCACC GCCAGGCTCC

2901 CCACCAGCCT GCAGCGAAAA AAAAAAAAAA AAAAAA

BLAST Results

No BLAST result

Medlme entries

No Medlme entry

Peptide information for frame 1

ORF from 172 bp to 1047 bp; peptide length: 292 Category: similarity to unknown protein Prosite motifs: WW DOMAIN 1 (19-24)

1 MYQGEFGLNM KLGYGKFSWP TGESYHGQFY RDHCHGLGTY MWPDGSSFTG

51 TFYLSHREGY GTMYMKTRLF QTHCHNDIVN LLLDCGADVN KCSDEGLTAL

101 SMCFLLHYPA QSFKPNVAER TIPEPQEPPK FPVVPILSSS FMDTNLESLY

151 YEVNVPSQGS YELRPPPAPL LLPRVSGSHE GGHFQDTGQC GGSIDHRSSS

201 LKGDSPLVKG SLGHVESGLE DVLGDTDRGS LCSAETKFES NLCVCDFSIE

251 LSQAMLERSA QSHSLLKMAS PSPCTSSFDK GTMRRMALSM IE

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_23nl6, frame 1

TREMBL:AB005902_1 product: "AtPIP5Kl"; Arabidopsis thaliana mRNA for AtPIP5Kl, complete eds., N = 2, Score = 138, P = 1. le-06

TREMBL :AF019380_1 product: "putative phosphatιdylιnosιtol-4-phosphate 5-kmase"; Arabidopsis thaliana putative phosphatιdylmosιtol-4-phosphate 5-kιnase mRNA, complete eds., N = 2, Score = 138, P = 1.4e-06

PIR:T02098 probable phosphatιdylιnosιtol-4-phosphate 5-kιnase - Arabidopsis thaliana, N = 2, Score = 135, P = 6.7e-06

>TREMBL:AB005902_1 product: "AtPIP5Kl"; Arabidopsis thaliana mRNA for AtPIP5Kl, complete eds. Length = 683

HSPs:

Score = 138 (20.7 bits), Expect = 1. le-06, Sum P(2) = 1. le-06 Identities = 23/61 (37%), Positives = 35/61 (57%)

Query: 1 MYQGEFGLNMKLGYGKFSWPTGESYHGQFYRDHCHGLGTYMWPDGSSFTGTFYLSHREGY 60

MY+G++ G GKFSWP+G +Y G+F G GT+ DG ++ GT+ + G+ Sbjct: 34 MYEGDWKRGKASGKGKFSWPSGATYEGEFKSGRMEGFGTFTGADGDTYRGTWVADRKHGH 93

Query: 61 G 61

G Sbjct: 94 G 94 Score = 112 (16.8 bits), Expect = 9.7e-04, Sum P(2) = 9.7e-04 Identities = 19/51 (37%), Positives = 27/51 (52%)

Query: 12 LGYGKFSWPTGESYHGQFYRDHCHGLGTYMWPDGSSFTGTFYLSHREGYGT 62

+G GK+ W G Y G + R G G + WP G+++ G F EG+GT Sbjct: 22 IGSGKYLWKDGCMYEGDWKRGKASGKGKFSWPSGATYEGEFKSGRMEGFGT 72

Score = 97 (14.6 bits), Expect = 4.4e-02, Sum P(2) = 4.3e-02 Identities = 19/60 (31%), Positives = 32/60 (53%)

Query: 2 YQGEFGLNMKLGYGKFSWPTGESYHGQFYRDHCHGLGTYMWPDGSSFTGTFYLSHREGYG 61

Y+GEF G+G F+ G++Y G + D HG G + +G + GT+ + ++G G Sbjct: 58 YEGEFKSGRMEGFGTFTGADGDTYRGTWVADRKHGHGQKRYANGDFYEGTWRRNLQDGRG 117

Score = 93 (14.0 bits), Expect = 1.2e-01, Sum P(2) = l.le-01 Identities = 18/62 (29%), Positives = 34/62 (54%)

Query: 2 YQGEFGLNMKLGYGKFSWPTGESYHGQFYRDHCHGLGTYMWPDGSSFTGTFYLSHREGYG 61

Y+G + + K G+G+ + G+ Y G + R+ G G Y+W +G+ +TG + + G G Sbjct: 81 YRGTWVADRKHGHGQKRYANGDFYEGTWRRNLQDGRGRYVWRNGNQYTGEWRIGVISGKG 140

Query: 62 TM 63

+ Sbjct: 141 LL 142

Score = 91 (13.7 bits), Expect = 2.0e-01, Sum P(2) = 1.8e-01 Identities = 18/51 (35%), Positives = 24/51 (47%)

Query: 2 YQGEFGLNMKLGYGKFSWPTGESYHGQFYRDHCHGLGTYMWPDGSSFTGTF 52

Y GE+ + + G G WP G Y G + G G + W DGSS G + Sbjct: 127 YTGEWRIGVISGKGLLVWPNGNRYEGLWENGIPKGNGVFTWSDGSSCVGAW 177

Score = 90 (13.5 bits), Expect = 2.6e-01, Sum P(2) = 2.3e-01 Identities = 17/60 (28%), Positives = 31/60 (51%)

Query: 2 YQGEFGLNMKLGYGKFSWPTGESYHGQFYRDHCHGLGTYMWPDGSΞFTGTFYLSHREGYG 61

Y+G + N++ G G++ W G Y G++ G G +WP+G+ + G + +G G Sbjct: 104 YEGTWRRNLQDGRGRYVWRNGNQYTGEWRIGVISGKGLLVWPNGNRYEGLWENGIPKGNG 163

Score = 45 (6.8 bits), Expect = 1. le-06, Sum P(2) = 1. le-06 Identities = 14/62 (22%), Positives = 26/62 (41%)

Query: 215 VESGLEDVLGDTDRGSLCSAETKFESNLCVCDF—SIELSQAMLERSAQSHSLLKMASPS 272

V+SG + G+ +C E+ E+ CD ++E S +R + + + Sbjct: 205 VDSGAGSLGGEKVFPRICIWESDGEAGDITCDIIDNVEASMIYRDRISVDRDGFRQFKKN 264

Query: 273 PC 274 PC

Sbjct: 265 PC 266

Pedant information for DKFZphfbr2_23nl6, frame 1

Report for DKFZphfbr2_23nl6.1

[LENGTH] 292

[MW] 32214.44

[pi] 5.51

[HOMOL] TREMBL:AB005902_1 product: "AtPIP5Kl"; Arabidopsis thaliana mRNA for AtPIP5Kl, complete eds. 7e-08

[BLOCKS] BL01137A Hypothetical YBL055c/y jV family proteins

[PROSITE] WW_DOMAIN_l 1

[PROSITE] MYRISTYL 5

[PROSITE] CK2_PHOSPHO_SITE 7

[PROSITE] PKC_PHOSPHO_SITE 5

[KW] Alpha_Beta

[KW] LOW_COMPLEXITY 4.11 %

SEQ MYQGEFGLNMKLGYGKFSWPTGESYHGQFYRDHCHGLGTYMWPDGSSFTGTFYLSHREGY SEG

PRD cccccccccccccccceeeccccccccccccccccccccccccccccccceeeeeccccc

SEQ GTMYMKTRLFQTHCHNDIVNLLLDCGADVNKCSDEGLTALSMCFLLHYPAQSFKPNVAER SEG

PRD cccchhhhhheeeccccchhhhhcccccccccccccchhhhhhhhhccccccccccceee

SEQ TIPEPQEPPKFPVVPILSSSFMDTNLESLYYEVNVPSQGSYELRPPPAPLLLPRVSGSHE SEG xxxxxxxxxxx PRD eccccccccceeeeeeeccccccccccceeeeeecccccccccccccccccccccccccc

SEQ GGHFQDTGQCGGSIDHRSSSLKGDSPLVKGSLGHVESGLEDVLGDTDRGSLCSAETKFES SEG PRD cccccccccccccccccccccccccceeecccccccccccccccccccccceeeeecccc

SEQ NLCVCDFSIELSQAMLERSAQSHSLLKMASPSPCTSSFDKGTMRRMALSMIE SEG PRD cccccchhhhhhhhhhhhhhhhhhhhcccccccccccccccchhhhhhhccc

Prosite for DKFZphfbr2_23nl6.1

PS00005 55->58 PKC PHOSPHO SITE PDOC00005

PS00005 112->115 PKC PHOSPHO- ^"SITE PDOC00005

PΞ00005 200->203 PKC PHOSPHO^{" "}SITE PDOC00005

PS00005 226->229 PKC PHOSPHO^{" "}SITE PDOC00005

PS00005 282->285 PKC PHOSPHO ^"SITE PDOC00005

PS00006 55->59 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 121->125 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 140->144 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 144->148 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 217->221 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 236->240 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 276->280 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00008 45->51 MYRISTYL PDOC00008

PS00008 86->92 MYRISTYL PDOC00008

PS00008 177->183 MYRISTYL PDOC00008

PS00008 188->194 MYRISTYL PDOC00008

PS00008 229->235 MYRISTYL PDOC00008

PS01159 19->44 WW DOMAIN 1 PDOC50020

(No Pfam data available for DKFZphfbr2_23nl6.1)

DKFZphfbr2_23o24

group: brain derived

DKFZphfbr2_23o24 encodes a novel 139 amino acid protein with similarity to CAAX-box proteins.

The CAAX box is a prenyl group binding site found in a number of eukaryotic proteins, such as which is found in Ras- and ras-like proteins such as Rho, Rab, Rac, Ral, and Rap, as well as in nuclear lamins A and B, some G protein alpha and gamma subunits and some dnaJ-like proteins. These proteins are posttranslationally modified at this site by the attachment of either a farnesyl or a geranyl-geranyl group to a cysteine residue.

No informative BLAST results; no predictive prosite, pfam or SCOP motife

The new protein can find application in studying the expression profile of brain-specific genes . similarity to lectins complete cDNA, complete eds, EST hits

Sequenced by AGOWA

Locus: unknown

Insert length: 3564 bp

Poly A stretch at pos. 3541, no polyadenylation signal found

1 GAATGGCTCC GCAGATGGCC GGCACTGAGA GCCAGCAAGA AGCGGAGGAG 51 ATGGGCCTTC AGCAGGGGGT TGCGGGGGGA GCTTTAAACT GAGCCCTGTA

101 AACATGGCAG AACTGCTCAG TGGGAGACTC TCAGCACAGA CGGTCATGGG

151 GAAGTGAGTG CAGTTCATTT GTAATCTTGT TGTCGAGTTC TGGGTTTTTT

201 TTGTTTGTTT CGTAACTTTA AAGGTATGCA CTTTATATAG ATTTATTTAT

251 TTGCTGGGAC CGTTACTCAG AGTTCCTAGA AATGTACACA GCTTTTTTAC

301 CAGGGTTACT CCTCAGAATC ACTTGTCACT TCTTTAAATG AATGAATGAA

351 TGTGCCAGGC CCTATGCCTG GAGGTTGGGA GCTTCATCTA CATCACATTC

401 TAACAGGTGA CCACTGGGGT AAGCACTGTG TGACTGCAAA GCCAGGGTGT

451 GTTTCCATCA ACACCCAGAT GACCGTGCCT ATGTGCCCCT GTTGTCCTCC

501 CTCCAGGACT GCCTCCTCAC CCCACCCCTT TCTGCAGCTC CTCATCTAAA

551 CATCTCGCCT GGTGAGGTCA CGGCTTAGCC TGTTGGCCAG TGGCCCCACC

601 ACCATCCTTC CCCCTGTGCA GATTGGAGGA GGCCAGGTCT CTCCCCTTAG

651 CTCCTATGTC CCCTTCACCC CCCATGGCAC AGATGAGACA TTCACAGAGT

701 TTGCAGATGA TGGAAGAGAA GACTCCAGGT TGCCAGGTGT GTCCACTCTC

751 AGGAACCCCC AGCCCAAGCC TCACTGCTCG TGTTCCCAGC CAACCCCAGC

801 ACGGGGGATA CGCCGGTGCT GTTTCCCTGC TCAGATACAA CCAGTTACCA

851 GAAACGACCT CACCCCTCCA ACCACTTTCC AAGGTGCCAG GACAGAGAAG

901 CCCTTCACTG GCCCACCCAG GGCAGTTGAC AGAGGGATGC CCTCCTTGGA

951 GGGGAGCCTC ACCTCTACCC ACAGGGCCGC GGCCTTGTCC TGGATTCTCA 1001 CCGGGGCAGT CACGTCAGGA TGGAGAGGTC CCATGTCAGC CAGTTCTTTG 1051 GTGGGGGTCA TGTAGTCTGA AATGACCTGC CGATGGTCCA GGCTGAGCCA 1101 GGGAAGCTGA GCCTGGGTGC CTTTTTGGTG CCTACTCTGA CTTGAGTTGG 1151 ATTCATGCCA CAGACCCACC TTCTTGAGCA ACAACACATA TAGCCACCAA 1201 CACAAGAGCC AGGCACACAC TGAGCAGAGA AAGTCCCTGT CGCCTCACCA 1251 CCCAAAAACT CCAGCTTTGC AGAGACCAAG GTTCTTCTCT ACCTTTGCAG 1301 AAGCCTCTGT GACCAAACCC GGAGCTTGCC CTTCTGAGGC CTCTAGCATT 1351 TCTCCAGGTG TTTTTCAGAG GACTTGGTTT AAATTTGTTC ACCCCAAATG 1401 TGGTCTTTCC CGGATCATGA AAGGATCTGC CGCAAAGGTG AATCTGAGTC 1451 TCCTCAGAGT CATATGAGAC TGAAACTGCT TATAACATTT CCGTGACCTA 1501 ATAAGTCTTC CAAAAATGTA GGGTATTAAG AGTTTAGTGA CATTAAAAAG 1551 TTTAGTCGAA AATATCGTGA TTCAGGTATA TTTAGACATT TGATTCATGC 1601 CAAATTGCCA CTGTTAACAG AAAACACACC CCAAGCACAT TAATGCCTAG 1651 ATATTTCAAA CCCTTTTCTG CCCACACATT CTTAAAAATA ATATACTGAG 1701 AAATCTATAT ACAGGTTTTT TTTTAATTAG CTTGGAAAAG AGCAGTTGTA 1751 TTCTGTTTGA ACAGCTGCTA ATGTCAATTC CTGTGGGAAG AAAGACCAAA 1801 GAACATGGAG TTACACCAAG AATTTTAAAA CAAAGACGCT GTCCCTTTCC 1851 TGAGCACCGT GCAGCCAAGA CTGAGAGATC AGTCTGAGAC CTGTGATTAA 1901 GGAGTGTTTT CTACATAGCG TATAATTATG GAGCCACACA AGTGGGCCAT 1951 TACTCTGTTG AGTGCTTCAT GTTTGAGGTA TTTTCGTGTT CCAACTTACA 2001 TTAAAGTGTT TATAAAACAG GAAAAATCCA CGAGCAGGTA TTGACACTAT 2051 CCATATTAGA TCATCACAAA ATTATATATA TAGCAGAGTC ATAAACAATG 2101 AGAAACGGTC TTCCCACACT TGCTTTAAAT GGCCATGACC TAGTGTTTAG 2151 GGAAAGCAGT AAAATCAGCG AGGAGCTCGT GGGAAAAATG AGACGGGCCC 2201 TGAGGGGGTG ACTCATGGGC CAAGCAGGGC CACACAGGTA CCAGGCCGCC ₂251 ACGTCCTCTC CTGCCTCTCA CTCTCTGGAG ACTGGACTTC CTTTACTGCC 2301 TCCTTTCTGA CATTTCCTAG ACATCAGACT TTGCTACTTA GTACACAAAC 2351 GGGGTTCCCT TTTAAATTTG TTCACTCTAG TTAGCATTTG CAGAAGCTGT 2401 GAAAAATTAC AGAGAGATGA TGTGTTGGGT AAGAGATGGT TTAAAAGTCC 2451 AGCTTGCTGT TTTTCATTAA GTGTCTTGAA AATGAGTAAG TGGCGTTCCT

2501 GGAGGGGAAC AATCATATAA TTCCGCAGGG TGGGTCTAAA CTTGTTTTCT

2551 GATAGTGTTT AGCAGCTCAT GGCTCTGAGG GCACCTGATA ACACAGCAGC

2601 CAGGCGCTGA TGAGAAGTGT GTGCCAGACA GACCCGAGTG TGGCTTGGCT

2651 CTTGCCTTAT GTTCCTTTCT CTGTTCAGAG AAGCGTGAGA TGAGATTTTG

2701 TGATTATATT GCACTCCTTG GGCTGACTTT CCCATGCACA GAATGTTTTA

2751 CACATCCTGA TAGCTGAGCT GAAAATGCAA AGAGAAGGGA AAATGCCTTA

2801 AATTGTTCTG GCTAATTTAG AAGCAGCAGG CCTTGGAAGT CTTTGTCCTG

2851 TGTCCCTGAA CAAATCTTAT GGGAGCTCTG GTACCTATGC CAGAAAATGC

2901 ACATAGGCAC AACACTTTTA CATACACGTT CACACACCCC ACCCTTATGG

2951 AGAACTTTTT TCTAAATAAG AGAAAGAAAA ATTTTAAGAC TTACAAGTTA

3001 TGTTTAGGTA TTTTACATGG TTCAGAAAAC AAGACATGAA GCGGTATAAA

3051 CTGAGAAGTC TTGTTCCCAC AACCCCACGT GCCAGGTACA CATAACCATT

3101 TTTATTCACC TCTAGCTTGT GCTTCCAATG TTTGTTAGGC ATATGTAAAT

3151 AAGTGAATAG ATAAGCATTT CTCCCTCCTT TTGCTGACAT GAGTGGTGGC

3201 ATGTTTTGCC CCTGGCTTTT ATCCCTTGAC CCCATTCCAG TACCTAGAGA

3251 CCTGCTTCAT TTTTTTAGAT GTGTAATACT TCATGTGTGC GTGTGCCTTA

3301 GTGATTAACT CGTGCACTGT GCAGGGACAT CGGGCTGGGA TCAGTTTGTT

3351 CACTGATATA TACAGCGCTG CGGGAGATAC CCTCACATGT GTATCATTTG

3401 GTCCATGTGC AGGTGTGTCT GGAAGATAGA ATTCTAGGCG TAGAATTGAT

3451 AGGTTAAATG TATTTATAGG GAAAAAATCA ATATAAAACT TTGCGTGTAA

3501 TGATATTTGC GTGCTTTTTT TTTTAATTTT TTTACCCAAA TAGTAAAAAA

3551 AAAAAAAAAA AAAA

BLAST Results

No BLAST result

Medline entries

No Medlme entry

Peptide information for frame 2

ORF from 656 bp to 1072 bp; peptide length: 139 Category: similarity to known protein

1 MSPSPPMAQM RHSQSLQMME EKTPGCQVCP LSGTPSPSLT ARVPSQPQHG 51 GYAGAVSLLR YNQLPETTSP LQPLSKVPGQ RSPSLAHPGQ LTEGCPPWRG 101 ASPLPTGPRP CPGFSPGQSR QDGEVPCQPV LWWGSCSLK

BLASTP hits

Entry CEEGAP7_1 from database TREMBL: gene: "EGAP7.1"; Caenorhabditis elegans cosmid EGAP7.

Score = 123, P = 2.3e-07, identities = 35/103, positives = 44/103

Entry MMBPC35_1 from database TREMBL:

Mouse carbohydrate binding protein 35 mRNA, ₃' end.

Score = 113, P = 2.2e-06, identities = 40/103, positives = 44/103

Entry A28651 from database PIR: galactose-specific lectin - mouse >TREMB : MMMAC2A_1 Mouse mRNA for

Mac-2 antigen

Score = 113, P = 2.2e-06, identities = 40/103, positives = 44/103

Alert BLASTP hits for DKFZphfbr2_23o24, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_23o24, frame 2

Report for DKFZphfbr2_23o24.2

[LENGTH] 139

[MW] 14748.91

[pi] 8.90

[PROSITE] PRENYLATION 1 [PROSITE] MYRISTYL 1

[PROSITE] CK2_PHOSPHO_SITE 1

[PROSITE] PROKAR_LIPOPROTEIN 1

[PROSITE] PKC_PHOSPHO_SITE 1

[KW] All_Alpha

SEQ MSPSPPMAQMRHSQSLQMMEEKTPGCQVCPLSGTPSPSLTARVPSQPQHGGYAGAVSLLR PRD cccchhhhhhhhhhhhhhhhhccccccccccccccccccccccccccccccchhhhhhhh

SEQ YNQLPETTSPLQPLSKVPGQRSPSLAHPGQLTEGCPPWRGASPLPTGPRPCPGFSPGQSR PRD hhcccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ QDGEVPCQPVLWWGSCSLK PRD ccccccccccccccccccc

Prosite for DKFZphfbr2_23o24.2

PS00005 40->43 PKC_PHOSPHO_SITE PDOC00005

PS00006 119->123 CK2_PHOSPHO_SITE PDOC00006

PS00008 50->56 MYRISTYL PDOC00008

PS00013 126->137 PROKAR_LIPOPROTEIN PDOC00013

PS00294 136->140 PRENYLATION PDOC00266

(No Pfam data available for DKFZphfbr2_23o24.2)

DKFZphfbr2_23o5

group: brain derived

DKFZphfbr2_23o5 encodes a novel 360 ammo acid protein with no known similarity

No informative BLAST results; no predictive prosite, pfam or SCOP motife

The new protein can find application in studying the expression profile of brain-specific genes . unknown potential start at Bp 24 matchs Kozak consensus ANNatgG

Sequenced by AGOWA

Locus: /map="7q21-q22"

Insert length: 1736 bp

Poly A stretch at pos. 1714, polyadenylation signal at pos. 1680

1 GGGGGAGGAT CAAAGTAGGC AAGATGGCGT CGAGCGGCGG GGAGCCAGGG

51 AGTTTATTTG ATCACCACGT CCAGAGGGCG GTATGCGACA CACGGGCCAA

101 ATATCGAGAG GGACGACGGC CTCGTGCTGT GAAGGTATAT ACAATCAATT

151 TGGAATCTCA GTACTTATTA ATACAAGGAG TTCCTGCTGT GGGAGTCATG

201 AAGGAATTAG TTGAGCGATT CGCTTTATAT GGTGCAATTG AACAGTACAA

251 TGCTCTAGAT GAATACCCAG CAGAAGACTT TACTGAAGTT TATCTTATTA

301 AATTTATGAA CTTACAAAGT GCAAGGACAG CCAAGAGAAA AATGGATGAA

351 CAGAGTTTCT TCGGTGGATT GCTTCATGTG TGCTATGCTC CAGAATTTGA

401 AACAGTTGAA GAAACTAGAA AAAAACTACA AATGCGGAAG GCATATGTAG

451 TAAAAACTAC TGAAAATAAA GACCATTACG TGACAAAGAA GAAATTGGTT

501 ACAGAGCATA AAGACACAGA GGATTTTAGA CAAGACTTCC ACTCAGAGAT

551 GTCTGGATTT TGTAAAGCTG CTTTGAACAC TTCTGCAGGG AACTCAAATC

601 CTTATCTTCC GTATTCCTGT GAATTGCCTT TATGTTATTT CTCCTCAAAA

651 TGTATGTGTT CATCCGGGGG ACCTGTAGAC AGAGCACCAG ACTCCTCTAA

701 GGATGGTAGA AACCATCATA AAACAATGGG GCATTATAAC CACAATGACT

751 CTTTGCGGAA AACACAGATA AACTCTTTGA AAAACTCAGT GGCCTGCCCT

801 GGTGCACAAA AGGCTATTAC GTCTTCAGAG GCAGTTGACA GATTTATGCC

851 TAGGACAACA CAACTGCAGG AGCGCAAAAG AAGAAGAGAA GATGATCGTA

901 AACTTGGAAC TTTTCTTCAA ACAAACCCAA CTGGTAATGA GATTATGATT

951 GGACCTCTGT TACCAGACAT CTCTAAAGTG GATATGCACG ATGACTCATT

1001 GAATACAACG GCGAATTTAA TTCGGCATAA ACTTAAAGAG GTATTTCATC

1051 TGTGCCAAAG CCTCCAGAGG ACAAGCCAGA AGATGTACAT ACAAGTCATC

1101 CATTAAAACA AAGAAGAAGA ATATAGAGTG CCAGCAGCAA CTTAGTATTT

1151 TCTAAAAAGA ACATTTATTA TTTATTTTTA GCCTGTCATT TTAATTCTTC

1201 AAGAGATTTT ACTGCTGGTA TTTTTTGATG CACTCCTCTT TGTAATTTCA

1251 TTCAAGCCAT TTGTCTAAAG TCATTTCTTT GTTTTTTGGG AGATGGAGTC

1301 TTGCTCTGTT GCCCAGGCTG GAATGCAGTG GCGTGATCTC GGCTCACTGC

1351 AACCTCCACC TCCCGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCTGAGT

1401 ATCTGGGATT ACAGGCGTGC ACCACCATGC CTGGCTAAGT TTTGTGTTTT

1451 TTTTAGTAGA GATGGGTTTT CACCATATTG GTCAGGCTGG TCTCGAACTC

1501 CTGACCTTGT GATACACCTG CCTCAGCCTC CCAAAGGGAT GAGCCACCGC

1551 GCCTGGCCCA TTTCTTCTTT TTTTGACCCA TACTTAATGT TGCAGAAACT

1601 ATTCTTGTCA TAACATTATC TCTCATGTAC AGTAATTATA TGTAAATTAA

1651 TTGAAGCAAA TATGGAAACT TTACAATAGA AATAAAGATA GGCAGCCAGC

1701 GTCTGTTTCC AATTATAAAA AAAAAAAAAA AAAAAA

BLAST Results

Entry AC005156 from database EMBL:

Homo sapiens PAC clone DJ1099C19 from 7q21-q22, complete sequence.

Score = 2897, P = 2.4e-154, identities = 583/586

2 exons covering Bp 465-17 3

Medline entries

No Medline entry

Peptide information for frame 3 ORF from 24 bp to 1103 bp; peptide length: 360 Category: similarity to unknown protein

1 MASSGGEPGS LFDHHVQRAV CDTRAKYREG RRPRAVKVYT INLESQYLLI 51 QGVPAVGVMK ELVERFALYG AIEQYNALDE YPAEDFTEVY LIKFMNLQSA 101 RTAKRKMDEQ SFFGGLLHVC YAPEFETVEE TRKKLQMRKA YVVKTTENKD 151 HYVTKKKLVT EHKDTEDFRQ DFHSEMSGFC KAALNTΞAGN SNPYLPYSCE 201 LPLCYFSSKC MCSSGGPVDR APDSSKDGRN HHKTMGHYNH NDSLRKTQIN 251 SLKNSVACPG AQKAITSΞEA VDRFMPRTTQ LQERKRRRED DRKLGTFLQT 301 NPTGNEIMIG PLLPDISKVD MHDDSLNTTA NLIRHKLKEV FHLCQSLQRT 351 SQKMYIQVIH

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_23o5, frame 3

TREMBL:AC005824_10 gene: "F15K20.11"; Arabidopsis thaliana chromosome II BAC F15K20 genomic sequence, complete sequence., N = 2, Score = 114, P = 3.6e-ll

>TREMBL:AC005824_10 gene: "F15K20.11"; Arabidopsis thaliana chromosome II BAC F15K20 genomic sequence, complete sequence. Length = 227

HSPs:

Score = 114 (17.1 bits), Expect = 3.6e-ll, Sum P(2) = 3.6e-ll Identities = 21/41 (51%), Positives = 29/41 (70%)

Query: 103 AKRKMDEQSFFGGLLHVCYAPEFETVEETRKKLQMRKAYVV 143

AKRK+DE SF G L + YAPE+E V +T+ KL+ R+ V+ Sbjct: 51 AKRKLDESΞFLGNRLQISYAPEYENVNDTKDKLESRRKEVL 91

Score = 107 (16.1 bits), Expect = 2.6e-10, Sum P(2) = 2.6e-10 Identities = 50/191 (26%), Positives = 83/191 (43%)

Query: 103 AKRKMDEQSFFGGLLHVCYAPEFETVEETRKKLQMRKAYVVKTTENKDHYVTKKKLVTEH 162

AKRK+DE SF G L + YAPE+E V +T+ KL+ R+ V+ + T + VT+ Sbjct: 51 AKRKLDESSFLGNRLQISYAPEYENVNDTKDKLESRRKEVLARLNPQKEKSTSQ—VTKL 108

Query: 163 KDTEDFRQDFHSEMΞGFCKAALNTSAGNSNPYLPYSCELPLCYFSSKCMCSSGGPVDRAP 222

+ D S + + GN+ P S + YF+S M + V Sbjct: 109 AGPALTQTDNVSSQRREMEYQFHR—GNA-PVTRVSSDQE—YFASSSMNQTVKTV 159

Query: 223 DSSKDGRNHHKTMGHYNHNDSLRKTQINSLKNSVACPGAQKAITSSEAVDRFMPRTTQLQ 282

K + + + +H + ++ N + P +Q S R P ++Q+Q Sbjct: 160 -REKLNKTREENISSLSHCKQIEESG-NQKRLQ PSSQTQPEESGNQKRLQP-SSQIQ 213

Query: 283 -ERKRRREDDRK 293

+ KR R D+R+ Sbjct: 214 PDLKRTRVDNRR 225

Score = 102 (15.3 bits), Expect = 3.6e-ll, Sum P(2) = 3.6e-ll Identities = 22/55 (40%), Positives = 38/55 (69%)

Query: 26 KYREGRRPRAVKVYTINLESQYLLIQGVPAVGVMKELVERFALYGAIEQY—NALDE 80

+Y++ P AV+VYT+ ES+Y++++ VPA+G +L+ F YG +E++ LDE Sbjct: 3 RYKD-ETP-AVRVYTVCDESRYMIVRNVPALGCGDDLMRLFMTYGEVEEFAKRKLDE 57

Pedant information for DKFZphfbr2_23o5, frame 3

Report for DKFZphfbr2_23o5.3

[LENGTH] 360

[MW] 41105.85

[pi] 8.89

[HOMOL] TREMBL:AC005824_10 gene: "F15K20.11"; Arabidopsis thaliana chromosome II BAC

F15K20 genomic sequence, complete sequence. 5e-12

[PROSITE] AMIDATION 1

[PROSITE] MYRISTYL 2

[PROSITE] CK2 PHOSPHO SITE 7 [PROSITE] PKC_PHOSPHO_SITE 9

[PROSITE] ASN_GLYCOSYLATION 3

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 4.17

SEQ MASSGGEPGSLFDHHVQRAVCDTRAKYREGRRPRAVKVYTINLESQYLLIQGVPAVGVMK SEG PRD ccccccccceeeecceeeeehhhhhhhhhccccceeeeeeecccceeeeeeccccchhhh

SEQ ELVERFALYGAIEQYNALDEYPAEDFTEVYLIKFMNLQSARTAKRKMDEQSFFGGLLHVC SEG PRD hhhhhhhhhhhhhhhhhhccccccceeeeeeehhhhhhhhhhhhhhhhhccccccceeee

SEQ YAPEFETVEETRKKLQMRKAYVVKTTENKDHYVTKKKLVTEHKDTEDFRQDFHSEMΞGFC SEG PRD eccchhhhhhhhhhhhhhhhheeeeccccceeeeeeeeeeeccccchhhhhhhhhcccce

SEQ KAALNTSAGNSNPYLPYSCELPLCYFSSKCMCΞSGGPVDRAPDSSKDGRNHHKTMGHYNH SEG PRD eeeeccccccccccccccccccceeecccccccccccccccccccccccccccccccccc

SEQ NDSLRKTQINSLKNSVACPGAQKAITSSEAVDRFMPRTTQLQERKRRREDDRKLGTFLQT SEG xxxxxxxxxxxxxxx PRD cccceeeeccccccccccccceeeeecceeeeeccccchhhhhhhhhhhhccceeeeeec

SEQ NPTGNEIMIGPLLPDISKVDMHDDSLNTTANLIRHKLKEVFHLCQSLQRTSQKMYIQVIH SEG PRD cccccceeeecccccccccccccccccchhhhhhhhhhhhhhhhhhhhhcchhhhhhccc

Prosite for DKFZphfbr2_23o5.3

PS00001 185-M89 ASN_GLYCOSYLATION PDOC00001 PS00001 241->245 ASN_GLYCOSYLATION PDOC00001 PS00001 327->331 ASN_GLYCOSYLATION PDOC00001 PS00005 99->102 PKC_PHOSPHO_SITE PDOC00005 PS00005 102->105 PKC_PHOSPHO_SITE PDOC00005 PS00005 131->134 PKC_PHOSPHO_SITE PDOC00005 PS00005 154->157 PKC_PHOSPHO_SITE PDOC00005 PS00005 207->210 PKC_PHOSPHO_SITE PDOC00005 PS00005 224->227 PKC_PHOSPHO_SITE PDOC00005 PS00005 243->246 PKC_PHOSPHO_SITE PDOC00005 PS00005 251->254 PKC_PHOSPHO_SITE PDOC00005 PS00005 351->354 PKC_PHOSPHO_SITE PDOC00005 PS00006 4->8 CK2_PHOSPHO_SITE PDOC00006 PS00006 10->14 CK2_PHOSPHO_SITE PDOC00006 PS00006 127->131 CK2_PHOSPHO_SITE PDOC00006 PS00006 224->228 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 266->270 CK2_PHOSPHO_SITE PDOC00006 PS00006 303->307 CK2_PHOSPHO_SITE PDOC00006 PS00006 317->321 CK2_PHOSPHO_SITE PDOC00006 PS00008 5->ll MYRISTYL PDOC00008 PS00008 260->266 MYRISTYL PDOC00008 PS00009 29->33 AMIDATION PDOC00009

(No Pfam data available for DKFZphfbr2_23o5.3)

DKFZphfbr2_2a2

group: brain derived

DKFZphfbr2_2a2.3 encodes a novel 167 amino acid protein with weak similarity to human 52K autoantigen Ro/SS-A

The novel protein contains a C3HC4 Zinc finger "RING finger" motive.

This domain is probably involved in mediating protein-protein interactions.

Proteins containing a RING-finger are: mammalian V(D)J recombination activating protein

(RAG1), mouse rpt-1, human rfp, human 52 Kd Ro/SS-A protein and others.

No informative BLAST results; no predictive prosite, pfam or SCOP motife

The new protein can find application in studying the expression profile of bram-specific genes . similarity to 52K autoantigen Ro/SS-A - human complete cDNA, complete eds, few EST hits

Sequenced by Qiagen

Locus : unknown

Insert length: 1376 bp

Poly A stretch at pos. 1355, polyadenylatioif signal at pos. 1340

1 GGGGACTCCA AATTAGAAAG GGGACGTCTA GTGGGTTGCC CGGGAGGGGT

51 GGCGGGAGCG GTCCTGGAAA TAATCTGTCC TCTGTCGCCG GGAACTGGCG

101 AGGTAGTTCC TTCGCGGTGG AGAGACCTGG AATGGCCAAA TATCAAGGTG

151 AAGTTCAAAG TTTGAAACTG GATGATGATT CAGTTATAGA AGGAGTAAGC

201 GACCAAGTAC TTGTGGCAGT TGTGGTCAGT TTCGCTTTGA TTGCTACCCT

251 GGTATATGCA CTTTTCAGAA ATGTACATCA AAACATTCAC CCAGAAAACC

301 AGGAGCTAGT AAGGGTACTT CGAGAACAGC TTCAAACAGA ACAGGATGCA

351 CCTGCTGCCA CTCGACAGCA GTTCTACACT GACATGTACT GTCCCATCTG

401 CCTGCACCAA GCCTCCTTCC CGGTGGAGAC CAACTGTGGA CATCTTTTTT

451 GTGGTGCCTG CATTATTGCT TACTGGCGAT ATGGTTCATG GCTTGGGGCA

501 ATCAGTTGTC CAATCTGTAG ACAAACGGTA ACCTTACTCC TAACAGTATT

551 TGGTGAAGAT GATCAGTCTC AGGATGTTCT GAGATTGCAT CAGGATATTA

601 ATGATTATAA CCGGAGATTC TCAGGGCAAC CCTGATCTAT TATGGAGAGA

651 ATTATGGATC TACCCACTTT ACTGAGGCAT GCATTCAGGG AAATGTTTTC

701 AGTCGGGGGC CTTTTCTGGA TGTTTCGCAT CAGGATAATA CTTTGTTTAA

751 TGGGAGCTTT TTTCTATCTT ATATCACCTC TAGATTTTGT ACCTGAAGCC

801 TTGTTTGGAA TTCTAGGCTT TCTAGATGAT TTCTTTGTCA TCTTTTTATT

851 GCTTATCTAC ATCTCTATTA TGTATCGAGA AGTGATAACC CAAAGGCTAA

901 CTAGATGAAA AAGGAAACAA AACTGAGTTT ACTAGGATAT CTGAGCTAAT

951 GTAGAACATC AAACAGAAGG ACCCATGGCA GTATAAAGCA ATGAAGCAAT

1001 GGAGTATTAT CTCACAAATA TAAAACCACT ATAAGACAAA CATTTGATTA

1051 TCATTTGACA AATACCTAGG TATAACTGGA ATTTTCATGT TTGAAGTTCT

1101 AATATTAAGT TTAGAATTAT AATGATCTAC AGTTGTATCT TGATTCTATG

1151 TTGTCTGGAA AAAATATGGA ATTATATAAA AAGGGATGCT TTTATATATT

1201 TTTCTTTTCC CCAGAATTAC TTAGATTAAT TAGATGTATA GTAAAATATT

1251 GTTAAATGTC AGTTTATCCA TCTTATCCTT CTCAGCAGGT ACCTATATGA

1301 TAATATATAG CTGTGAAACT CATCTAAATA TTTTTGTTCC AATAAAATAT

1351 TATATACTAA AAAAAAAAAA AAAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 132 bp to 632 bp; peptide length: 167 Category: similarity to known protein Classification: unset Prosite motifs: ZINC FINGER C3HC4 (102-112)

1 MAKYQGEVQS LKLDDDSVIE GVSDQVLVAV VVSFALIATL VYALFRNVHQ

51 NIHPENQELV RVLREQLQTE QDAPAATRQQ FYTDMYCPIC LHQASFPVET

101 NCGHLFCGAC IIAYWRYGSW LGAISCPICR QTVTLLLTVF GEDDQSQDVL 151 RLHQDINDYN RRFSGQP

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_2a2, frame 3

TREMBL :CEY38F1A_8 gene: "Y38F1A.2"; Caenorhabditis elegans cosmid Y38F1A, N = 1, Score = 194, P = 2e-15

PIR:T05222 hypothetical protein F17I5.130 - Arabidopsis thaliana, N = 1, Score = 159, P = 1.4e-10

TREMBLNEW:AB025011_1 gene: "TRIF"; product: "Trif-d"; Mus musculus mRNA for Tπf-d, complete eds., N = 1, Score = 108, P = 2.6e-06

PIR:A37241 52K autoantigen Ro/SS-A - human, N = 1, Score = 115, P = 5e-05

>TREMBL:CEY38F1A_8 gene: "Y38F1A.2"; Caenorhabditis elegans cosmid Y38F1A Length = 283

HSPs:

Score = 194 (29.1 bits), Expect = 2.0e-15, P = 2.0e-15 Identities = 52/149 (34%), Positives = 78/149 (52%)

Query: 16 DSVIEGVSDQVLVAVVVSFALIATLVYALFRNVHQNIHPENQELVRVLREQLQTEQDAPA 75

D +E ++ Q+ +A+ V F ++ + A Q E R Q+ T++ Sbjct: 41 DPDVE-LATQITMAIAVIF-IVKAIFDAWQSRRRQRAASRMDENAE--RNQIITQRRISE 96 Query: 76 ATRQQFYTDMYCPICLHQASFPVETNCGHLFCGACIIAYWRYGSWLGA-ISCPICRQTVT 134

A Q + CPICL AΞFPV T+CGH+FC CII YW+ + C +CR T Sbjct: 97 ALHQSSHE CPICLANASFPVLTDCGHIFCCECIIQYWQQΞKAIVTPCDCAMCRSTFY 153 Query: 135 LLLTV FGEDDQSQDVLRLHQ-DINDYNRRFS 164

+LL V G +++ D ++ + I+DYNRRFS Sbjct: 154 MLLPVHWPTMGTSEETDDHIQENNIRIDDYNRRFS 188

Pedant information for DKFZphfbr2_2a2, frame 3

Report for DKFZphfbr2_2a2.3

[LENGTH] 167 [MW] 18941.65 [pi] 4.91 [HOMOL] TREMBL:CEY38F1A_8 gene: "Y38F1A.2" Caenorhabditis elegans cosmid Y38F1A le-13

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YDR265w] le-04

[FUNCAT] 30.19 peroxisomal organization [S. cerevisiae, YDR265w] le-04

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YLR323c] 2e-04

[BLOCKS] BL00518 Zinc finger, C3HC4 type, proteins

[PROSITE] ZINC_FINGER_C3HC4 1

[PFAM] Zinc finger, C3HC4 type (RING finger)

[KW] Irregular

[KW] 3D

[KW] LOW COMPLEXITY 6.59 %

SEQ MAKYQGEVQSLKLDDDSVIEGVSDQVLVAVVVSFALIATLVYALFRNVHQNIHPENQELV

SEG xxxxxxxxxxx lrmd-

SEQ RVLREQLQTEQDAPAATRQQFYTDMYCPICLHQASFPVETNCGHLFCGACIIAYWRYGSW SEG lrmd HHHHHHBTTTTTEETTTEEEETTTEEEEHHHHH HHHHH

SEQ LGAISCPICRQTVTLLLTVFGEDDQSQDVLRLHQDINDYNRRFSGQP SEG lrmd- HCCB-TTTTT.

Prosite for DKFZphfbr2_2a2.3

PS00518 102->112 ZINC FINGER C3HC4 PDOC00449

Pfam for DKFZphfbr2_2a2.3

HMM_NAME Zinc fmger, C3HC4 type (RING finger)

HMM *CPICFcTFQlDyPWPFdePmMlPCgHsFCypCIrrW CP

CPIC L+ P++++CGH+FC +CI+ + CP

Query 87 CPIC LHQ ASFPVETNCGHLFCGACIIAYWRYGSWLGAISCP 127

HMM mC*

+C Query 128 IC 129

DKFZphfbr2_2bl7

group: transmembrane protein

DKFZphfbr2_2bl7 encodes a novel 285 amino acid protein with similarity to D. melanogaster 30K protein .

The protein contains 3 transmembrane regions.

No informative BLAST results; no predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes and as a new marker for neuronal cells. similarity to Drosophila hypothetical 30K protein complete cDNA, complete eds, EST hits TRANSMEMBRANE 3

Sequenced by Qiagen

Locus : unknown

Insert length: 1426 bp

Poly A stretch at pos. 1345, polyadenylation signal at pos. 1330

1 GGGGGTATTT CCAAGGACTC CAAAGCGAGG CCGGGGACTG AAGGTGTGGG 51 TGTCGAGCCC TCTGGCAGAG GGTTAACCTG GGTCAAATGC ACGGATTCTC

101 ACCTCGTACA GTTACGCTCT CCCGCGGCAC GTCCGCGAGG ACTTGAAGTC

151 CTGAGCGCTC AAGTTTGTCC GTAGGTCGAG AGAAGGCCAT GGAGGTGCCG

201 CCACCGGCAC CGCGGAGCTT TCTCTGTAGA GCATTGTGCC TATTTCCCCG

251 AGTCTTTGCT GCCGAAGCTG TGACTGCCGA TTCGGAAGTC CTTGAGGAGC

301 GTCAGAAGCG GCTTCCCTAC GTCCCAGAGC CCTATTACCC GGAATCTGGA

351 TGGGACCGCC TCCGGGAGCT GTTTGGCAAA GATGAACAGC AGAGAATTTC

401 AAAGGACCTT GCTAATATCT GTAAGACGGC GGCTACAGCA GGCATCATTG

451 GCTGGGTGTA TGGGGGAATA CCAGCTTTTA TTCATGCTAA ACAACAATAC

501 ATTGAGCAGA GCCAGGCAGA AATTTATCAT AACCGGTTTG ATGCTGTGCA

551 ATCTGCACAT CGTGCTGCCA CACGAGGCTT CATTCGTTAT GGCTGGCGCT

601 GGGGTTGGAG AACTGCAGTG TTTGTGACTA TATTCAACAC AGTGAACACT

651 AGTCTGAATG TATACCGAAA TAAAGATGCC TTAAGCCATT TTGTAATTGC

701 AGGAGCTGTC ACGGGAAGTC TTTTTAGGAT AAACGTAGGC CTGCGTGGCC

751 TGGTGGCTGG TGGCATAATT GGAGCCTTGC TGGGCACTCC TGTAGGAGGC

801 CTGCTGATGG CATTTCAGAA GTACTCTGGT GAGACTGTTC AGGAAAGAAA

851 ACAGAAGGAT CGAAAGGCAC TCCATGAGCT AAAACTGGAA GAGTGGAAAG

901 GCAGACTACA AGTTACTGAG CACCTCCCTG AGAAAATTGA AAGTAGTTTA

951 CAGGAAGATG AACCTGAGAA TGATGCTAAG AAAATTGAAG CACTGCTAAA 1001 CCTTCCTAGA AACCCTTCAG TAATAGATAA ACAAGACAAG GACTGAAAGT 1051 GCTCTGAACT TGAAACTCAC TGGAGAGCTG AAGGGAGCTG CCATGTCCGA 1101 TGAATGCCAA CAGACAGGCC ACTCTTTGGT CAGCCTGCTG ACAAATTTAA 1151 GTGCTGGTAC CTGTGGTGGC AGTGGCTTGC TCTTGTCTTT TTCTTTTCTT 1201 TTTAACTAAG AATGGGGCTG TTGTACTCTC ACTTTACTTA TCCTTAAATT 1251 TAAATACATA CTTATGTTTG TATTAATCTA TCAATATATG CATACATGAA 1301 TATATCCACC CACCTAGATT TTAAGCAGTA AATAAAACAT TTCGCAAAAG 1351 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 1401 AAAAAAAAAA AAAAAAAAAA AAAAAA

BLAST Results

Entry HSG19630 from database EMBL: human STS A001T27. Score = 961, P = 1.2e-36, identities = 193/194

Medlme entries

No Medlme entry

Peptide information for frame 3

ORF from 189 bp to 1043 bp; peptide length: 285 Category: similarity to unknown protein 1 MEVPPPAPRS FLCRALCLFP RVFAAEAVTA DSEVLEERQK RLPYVPEPYY

51 PESGWDRLRE LFGKDEQQRI SKDLANICKT AATAGIIGWV YGGIPAFIHA

101 KQQYIEQSQA EIYHNRFDAV QSAHRAATRG FIRYGWRWGW RTAVFVTIFN

151 TVNTSLNVYR NKDALSHFVI AGAVTGSLFR INVGLRGLVA GGIIGALLGT

201 PVGGLLMAFQ KYSGETVQER KQKDRKALHE LKLEEWKGRL QVTEHLPEKI

251 ESSLQEDEPE NDAKKIEALL NLPRNPSVID KQDKD

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_2bl7, frame 3

PIR:JQ1024 hypothetical 30K protein (DmRP140 5' region) - fruit fly (Drosophila melanogaster), N = 1, Score = 312, P = 6.1e-28

>PIR:JQ1024 hypothetical 30K protein (DmRP140 5' region) - fruit fly (Drosophila melanogaster) Length = 261

HSPs:

Score = 312 (46.8 bits), Expect = 6.1e-28, P = 6.1e-28 Identities = 68/231 (29%), Positives = 125/231 (54%)

Query: 30 ADSEVLEERQKRLPYVPEPYYPESGWDRLRELFGKDEQQRISKDLANICKTAATAGIIGW 89

AD V +E + ++ E+G +RL+++F DE I +L ++ + +IG Sbjct: 23 ADEIVDKENKTYKAFLASKPPEETGLERLKQMFTIDEFGSIFSELNSVYQAGFLGFLIGA 82

Query: 90 VYGGIPAFIHAKQQYIEQSQAEIYHNRFDAVQSAHRAATRGFIRYGWRWGWRTAVFVTIF 149

+YGG+ A ++E +QA + + FDA + T F + G++WGWR +F T + Sbjct: 83 IYGGVTQSRVAYMNFMENNQATAFKSHFDAKKKLQDQFTVNFAKGGFKWGWRVGLFTTSY 142

Query: 150 NTVNTSLNVYRNKDALSHFVIAGAVTGSLFRINVGLRGLVAGGIIGALLGTPVGGLLMAF 209

+ T ++VYR K ++ ++ AG++TGSL+++++GLRG+ AGGIIG LG G + Sbjct: 143 FGIITCMSVYRGKSSIYEYLAAGSITGSLYKVSLGLRGMAAGGIIGGFLGGVAGVTSLLL 202

Query: 210 QKYSGETVQERKQKDRKALHELKLEEWKGRLQVTEHLPEKIESSLQEDEPE 260

K SG +++E ++ ++K RL E++ + + +++ PE

Sbjct: 203 MKASGTSMEE VRYWQYKWRLDRDENIQQAFKKLTEDENPE 242

Pedant information for DKFZphfbr2_2bl7, frame 3

Report for DKFZphfbr2_2bl7.3

[LENGTH] 285

[MW] 32177.88

[pi] 8.65

[HOMOL] PIR:JQ1024 hypothetical 30K protein (DmRP140 5' region) - fruit fly (Drosophila melanogaster) 7e-20

[PROSITE] MYRISTYL 7

[PROSITE] CK2_PHOSPHO_SITE 5

[PROSITE] ASN_GLYCOSYLATION 1

[KW] SIGNAL_PEPTIDE 25

[KW] TRANSMEMBRANE 3

[KW] LOW_COMPLEXITY 5.96 %

SEQ MEVPPPAPRSFLCRALCLFPRVFAAEAVTADSEVLEERQKRLPYVPEPYYPESGWDRLRE

SEG

PRD cccccccceeeeeeeeeehhhhhhhhhhhhhhhhhhhhhhhhhccccccccccchhhhhh

MEM

SEQ LFGKDEQQRISKDLANICKTAATAGIIGWVYGGIPAFIHAKQQYIEQSQAEIYHNRFDAV

SEG

PRD hhcccchhhhhhhhhhhhhhhhcccceeeeccccchhhhhhhhhhhhhhhhhhhhhhhhh

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ QSAHRAATRGFIRYGWRWGWRTAVFVTIFNTVNTSLNVYRNKDALSHFVIAGAVTGSLFR

SEG

PRD hhhhhhhhhhhccccccccceeeeeeeeccccccceeecccccccceeeeecccccceee

MEM MMMMMMMMMMMMMMMMMMMMMMMMMM M

SEQ INVGLRGLVAGGIIGALLGTPVGGLLMAFQKYSGETVQERKQKDRKALHELKLEEWKGRL SEG .. xxxxxxxxxxxxxxxxx PRD eecccccccccceeeeeccccccchhhhhhhhccchhhhhhhhhhhhhhhhhhhhhhhhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ QVTEHLPEKIESSLQEDEPENDAKKIEALLNLPRNPSVIDKQDKD SEG PRD ccccccccchhhhhccccccchhhhhhhhhhcccccceeeccccc

MEM

Prosite for DKFZphfbr2_2bl7.3

PS00001 153->157 ASN_GLYCOSYLATION PDOC00001 PS00006 53->57 CK2_PHOSPHO_SITE PDOC00006 PS00006 108->112 CK2_PHOSPHO_SITE PDOC00006 PS00006 216->220 CK2_PHOSPHO_SITE PDOC00006 PS00006 253->257 CK2_PHOSPHO_SITE PDOC00006 PS00006 277->281 CK2_PHOSPHO_SITE PDOC00006 PS00008 92->98 MYRISTYL PDOC00008 PS00008 172->178 MYRISTYL PDOC00008 PS00008 187->193 MYRISTYL PDOC00008 PS00008 191->197 MYRISTYL PDOC00008 PS00008 195->201 MYRISTYL PDOC00008 PS00008 199->205 MYRISTYL PDOC00008 PΞ00008 204->210 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_2bl7.3)

DKFZphfbr2 2b5

group: cell structure and motility

DKFZphfbr2_2b5 encodes a novel 957 amino acid protein with strong similarity to collagens.

The novel protein contains the typical (xxG)n repeat of collagen proteins and a

Pfam von Willebrand factor type A domain. Therefore, the protein seems to be a new collagen alpha chain.

The new protein can find application in modulation of connective tissue, bone and cartilage development and mamtainance. similarity to collagen proteins shows typical (xxG)n repeat of collagen proteins [PFAM] von Willebrand factor type A domain

Sequenced by Qiagen

Locus: /map="6"

Insert length: 4160 bp

Poly A stretch at pos. 4141, polyadenylation signal at pos. 4119

1 GGGGGCCCGC TGCAGGGAGA ACGGACTCCG GGCGGAGGGC AGCCAATCCG 51 TTTCAGCGCA GGTCTTGCTC GGGTTGGGCT TGCCACTGCC TGGAACATAC

101 CTGTCCCCCT GGCGCAACAC TCAGCTGGCT GCGACCGCAA CCCCGAGCCT

151 GGACACTGCG CCAGGAATCC TAAAACCAAA ATATTAGAAC GAAAACAGAA

201 ACATGGCTCA CTATATTACA TTTCTCTGCA TGGTTTTGGT GCTGCTTCTT

251 CAGAATTCTG TGTTAGCTGA AGATGGGGAA GTAAGATCAA GTTGTCG AC

301 TGCTCCGACA GATTTAGTTT TCATCTTAGA TGGCTCTTAT AGTGTTGGCC

351 CAGAAAACTT TGAAATAGTG AAAAAGTGGC TTGTCAATAT CACAAAAAAC

401 TTTGACATAG GGCCGAAGTT TATTCAAGTT GGAGTGGTTC AATATAGTGA

451 CTACCCTGTG CTGGAGATTC CTCTCGGAAG CTATGATTCA GGAGAACATT

501 TGACGGCAGC AGTGGAATCC ATACTCTACT TAGGAGGAAA CACAAAGACA

551 GGGAAGGCCA TCCAGTTTGC GCTCGATTAC CTTTTTGACA AGTCCTCACG

601 ATTTCTGACT AAGATAGCAG TGGTACTTAC GGATGGCAAG TCCCAAGATG

651 ACGTCAAGGA TGCAGCTCAA GCAGCAAGAG ATAGTAAGAT AACATTATTT

701 GCTATTGGTG TTGGTTCAGA AACAGAAGAT GCCGAACTTA GAGCTATTGC

751 CAACAAGCCT TCGTCTACTT ATGTGTTTTA TGTGGAAGAC TATATTGCAA

801 TATCCAAAAT AAGGGAAGTG ATGAAGCAGA AACTTTGTGA AGAATCTGTC

851 TGTCCAACAC GAATTCCAGT GGCAGCTCGT GATGAAAGGG GATTTGATAT

901 TCTTTTGGGT TTAGATGTAA ATAAAAAGGT TAAGAAAAGA ATACAGCTTT

951 CACCAAAAAA GATAAAAGGA TATGAAGTAA CATCAAAAGT TGATTTATCA

1001 GAACTCACAA GCAATGTTTT CCCAGAAGGT CTTCCTCCAT CATATGTATT

1051 TGTGTCTACT CAAAGATTTA AAGTCAAGAA AATTTGGGAT TTATGGAGAA

1101 TATTAACTAT TGATGGAAGG CCACAAATAG CAGTTACCTT AAATGGTGTG

1151 GACAAAATCT TATTATTTAC AACAACCAGC GTAATTAATG GCTCACAAGT

1201 GGTTACCTTT GCTAACCCTC AAGTTAAGAC GTTGTTTGAT GAAGGCTGGC

1251 ACCAAATTCG TCTCTTAGTA ACAGAACAAG ATGTGACTTT GTATATTGAT

1301 GACCAACAAA TTGAAAACAA GCCCTTACAT CCAGTTTTAG GGATCTTGAT

1351 CAATGGGCAA ACCCAAATTG GAAAATATTC TGGAAAAGAA GAAACTGTTC

1401 AGTTTGATGT CCAAAAGTTG CGAATCTACT GTGACCCAGA ACAGAACAAC

1451 CGGGAGACAG CATGTGAGAT TCCTGGATTT AATGGAGAGT GCCTTAATGG

1501 TCCCAGTGAT GTAGGTTCAA CTCCAGCTCC CTGTATTTGT CCTCCGGGAA

1551 AACCAGGACT TCAAGGCCCC AAAGGTGACC CTGGACTGCC TGGGAACCCT

1601 GGCTACCCTG GACAACCTGG TCAAGATGGT AAGCCTGGAT ATCAGGGAAT

1651 TGCAGGGACA CCAGGTGTTC CAGGATCTCC AGGAATACAA GGAGCTCGAG

1701 GACTACCAGG TTACAAAGGA GAACCAGGGC GAGATGGTGA CAAGGGTGAT

1751 CGTGGACTTC CTGGTTTTCC TGGGCTTCAT GGCATGCCAG GATCAAAGGG

1801 TGAAATGGGT GCCAAAGGAG ACAAAGGATC ACCTGGATTT TATGGCAAAA

1851 AGGGTGCAAA AGGTGAAAAG GGGAATGCTG GCTTCCCTGG CCTCCCTGGA

1901 CCTGCTGGAG AACCAGGAAG ACATGGAAAG GATGGATTAA TGGGTAGTCC

1951 CGGTTTCAAG GGAGAAGCAG GATCCCCTGG TGCTCCGGGG CAGGATGGAA

2001 CACGGGGAGA GCCTGGAATC CCAGGATTTC CTGGAAACCG AGGATTAATG

2051 GGCCAAAAGG GAGAAATTGG GCCTCCAGGA CAGCAAGGAA AAAAAGGAGC

2101 CCCAGGGATG CCTGGTTTAA TGGGAAGCAA TGGCTCACCA GGCCAGCCTG

2151 GAACACCGGG ATCTAAGGGA AGCAAAGGTG AACCTGGAAT TCAAGGGATG

2201 CCTGGGGCTT CAGGGCTCAA GGGAGAACCA GGAGCAACGG GTTCCCCAGG

2251 AGAACCAGGA TACATGGGTT TACCCGGGAT TCAAGGAAAA AAGGGGGACA

2301 AAGGAAATCA AGGTGAAAAA GGTATTCAGG GTCAAAAGGG AGAAAATGGA

2351 AGACAGGGAA TTCCAGGGCA ACAGGGAATT CAAGGCCATC ATGGTGCAAA

2401 AGGAGAGAGA GGTGAAAAGG GAGAACCTGG TGTCCGAGGT GCCATTGGAT

2451 CAAAAGGAGA ATCTGGGGTG GATGGCTTGA TGGGGCCCGC AGGTCCTAAG

2501 GGGCAACCTG GGGATCCAGG TCCTCAGGGA CCCCCAGGTT TGGATGGGAA

2551 GCCCGGAAGA GAGTTTTCAG AACAATTTAT TCGACAAGTT TGCACAGATG 2601 TAATAAGAGC CCAGCTACCA GTCTTACTTC AGAGTGGAAG AATTAGAAAT

2651 TGTGATCATT GCCTGTCCCA ACATGGCTCC CCGGGTATTC CTGGGCCACC

2701 TGGTCCGATA GGCCCAGAGG GTCCCAGAGG ATTACCTGGT TTGCCAGGAA

2751 GAGATGGTGT TCCTGGATTA GTGGGTGTCC CTGGACGTCC AGGTGTCAGA

2801 GGATTAAAAG GCCTACCAGG AAGAAATGGG GAAAAAGGGA GCCAAGGGTT

2851 TGGGTATCCT GGAGAACAAG GTCCTCCTGG TCCCCCAGGT CCAGAGGGCC

2901 CTCCTGGAAT AAGCAAAGAA GGTCCTCCAG GAGACCCAGG TCTCCCTGGC

2951 AAAGATGGAG ACCATGGAAA ACCTGGAATC CAAGGGCAAC CAGGCCCCCC

3001 AGGCATCTGC GACCCATCAC TATGTTTTAG TGTAATTGCC AGAAGAGATC

3051 CGTTCAGAAA AGGACCAAAC TATTAGTGTC TGATGCCTCA TTCAGCAGCC

3101 TAGGCATGGT GCTTTTTCTG TGGTCTTTTG CATCTCAGGA AGATAACCAA

3151 CAGTATCCCT TGAAAAGAAA CTTAAGTACC TCGGTGTTTT TATTTTTTTT

3201 TTCTTATGGA AAAAAATATA AAAGATCACA TATACTGATT TTAAAGGCTC

3251 CTCAGTCATT TGGAGCCCTT GGATTAGCAG CATTAATTAA ATCTCAAGGG

3301 TTTCTTGTAA AGTCCATTTA TGTTAATCAA AGTTGAATAT AAAAATCCAC

3351 CATTGCCTGT TAGCCAGTCA GTTTTAGTCA CTGTGAAATA TTTCACATTC

3401 AGCCTCCATG CAGTAGAGAT TTGAGTTTAA TTTCATGTCC ATGTGACTTT

3451 CATGTTTCCT ATCTCATAGC TCATGCTACT ACATAAGCCA AAACATGTAT

3501 CTCATCATTG GAAGTAAGAT CAGGGCTGAT ATTCACCTGG GATAGACAGT

3551 ATTGGTGAAC TACTCATTTA CTACAGTGTC TCAGCCTTGA TAAAGGGCAG

3601 TGGATTGCCT GTTGTTCGGT GTTGTGAATA GCACCTCTGA ATAAGATTAG

3651 AGTGTTTCTT AATTCATTTC AAACTCTAAA ATTAGATTAA TGGTGGTGCT

3701 AAGAAAGAGT ATTAATTACT TTGGGAATGG TCAAAATTAA CATTAAAAAC

3751 ATTTTAGACA AAAAGTTTCA TTGTACATTC AAAGAAAATG TAAGTTTGGA

3801 AGTACTAAAA GACTATTTTA TACTTGTTGA TTAATCGGAA TGTTTGTTGT

3851 ATGCCTTCAT TTTCCATTTC ACTTATATGT GCATGTCCAT ATATGTTAAT

3901 TTTCATTGTA GCAAAGCTAA TGGAAATAAA GCTAATGCTC TAGTTGAAAG

3951 AAAAGGAAAA CTCCTGAAAT CCTAGAATGT CTTGTTATTT TTAGCTGACT

4001 GTAAAATATT ATGAACAGTC TTTGTGTATT GTGCTTAATG CTTTTGTAAG

4051 AAACAGAATT TGAAATATTT CATCCTTGTC ATGCTCAAAA TTTTGTTACA

4101 TGCTTGTTAT TCAGAGTATA ATAAAGTTTT GTACAGGCCT GAAAAAAAAA

4151 AAAAAAAAAA

BLAST Results

Entry HS682J15 from database EMBLNEW:

Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone 682J15

Score = 6240, P = O.Oe+00, identities = 1256/1263

13 exons matching Bp 2015-4118

Entry HS708F5 from database EMBLNEW:

Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone 708F5

Score = 2775, P = 1.0e-221, identities = 739/912

10 exons matching Bp 5-1745

Medline entries

No Medlme entry

Peptide information for frame 2

ORF from 203 bp to 3073 bp; peptide length: 957 Category: similarity to known protein

1 MAHYITFLCM VLVLLLQNSV LAEDGEVRSS CRTAPTDLVF ILDGSYSVGP 51 ENFEIVKKWL VNITKNFDIG PKFIQVGVVQ YSDYPVLEIP LGSYDSGEHL 101 TAAVESILYL GGNTKTGKAI QFALDYLFDK SSRFLTKIAV VLTDGKSQDD 151 VKDAAQAARD SKITLFAIGV GSETEDAELR AIANKPSSTY VFYVEDYIAI 201 SKIREVMKQK LCEESVCPTR IPVAARDERG FDILLGLDVN KKVKKRIQLS 251 PKKIKGYEVT SKVDLSELTS NVFPEGLPPS YVFVSTQRFK VKKIWDLWRI 301 LTIDGRPQIA VTLNGVDKIL LFTTTSVING SQVVTFANPQ VKTLFDEGWH 351 QIRLLVTEQD VTLYIDDQQI ENKPLHPVLG ILINGQTQIG KYSGKEETVQ 401 FDVQKLRIYC DPEQNNRETA CEIPGFNGEC LNGPSDVGST PAPCICPPGK 451 PGLQGPKGDP GLPGNPGYPG QPGQDGKPGY QGIAGTPGVP GSPGIQGARG 501 LPGYKGEPGR DGDKGDRGLP GFPGLHGMPG SKGEMGAKGD KGSPGFYGKK 551 GAKGEKGNAG FPGLPGPAGE PGRHGKDGLM GSPGFKGEAG SPGAPGQDGT 601 RGEPGIPGFP GNRGLMGQKG EIGPPGQQGK KGAPGMPGLM GSNGSPGQPG 651 TPGSKGSKGE PGIQGMPGAS GLKGEPGATG SPGEPGYMGL PGIQGKKGDK 701 GNQGEKGIQG QKGENGRQGI PGQQGIQGHH GAKGERGEKG EPGVRGAIGS 751 KGESGVDGLM GPAGPKGQPG DPGPQGPPGL DGKPGREFSE QFIRQVCTDV 801 IRAQLPVLLQ SGRIRNCDHC LSQHGSPGIP GPPGPIGPEG PRGLPGLPGR 851 DGVPGLVGVP GRPGVRGLKG LPGRNGEKGS QGFGYPGEQG PPGPPGPEGP 901 PGISKEGPPG DPGLPGKDGD HGKPGIQGQP GPPGICDPSL CFSVIARRDP 951 FRKGPNY

BLASTP hits

Entry HSC0L7A1X_1 from database TREMBL: gene: "COL7A1"; product: "collagen type VII"; Homo sapiens (clones:

CW52-2, CW27-6, CW15-2, CW26-5, 11-67) collagen type VII intergenic region and (COL7A1) gene, complete eds.

Score = 949, P = 3.4e-122, identities = 237/553, positives = 281/553

Entry CA17_HUMAN from database SWISSPROT:

COLLAGEN ALPHA l(VII) CHAIN PRECURSOR (LONG-CHAIN COLLAGEN) (LC

COLLAGEN). >TREMBL: HSCOL7Al_l gene: "COL7A1"; product: "alpha-1 type

VII collagen"; Human alpha-1 type VII collagen (COL7A1) mRNA, complete eds .

Score = 949, P = 3.6e-122, identities = 237/553, positives = 281/553

Alert BLASTP hits for DKFZphfbr2_2b5, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_2b5, frame 2

Report for DKFZphfbr2_2b5.2

[LENGTH] 957

[MW] 99413.38

[pi] 8.49

[HOMOL] PIR:A40020 collagen alpha l(XII) chain precursor - chicken 9e-90

[BLOCKS] BL01119B Copper-fist domain proteins

[BLOCKS] BL00313B

[BLOCKS] BL01113A Clq domain proteins

[BLOCKS] BL00420A Speract receptor repeat proteins domain proteins

[SCOP] dlzoob_ 3.45.1.1.1 Integrin CDlla/CD18 (LFA-1) [Human (Horn 2e-58

[SCOP] dlido 3.45.1.1.2 Integrin CR3 (CDllb/CD18) , alpha subunit [Huma 8e-62

[EC] 3.1.1.7 Acetylcholmesterase 7e-24

[PIRKW] blocked amino end le-43

[PIRKW] duplication 7e-46

[PIRKW] cornea le-35

[PIRKW] lung 2e-40

[PIRKW] leukocyte le-42

[PIRKW] skin le-40

[PIRKW] transmembrane protein le-37

[PIRKW] cartilage 3e-59

[PIRKW] hydroxylysine 4e-62

[PIRKW] connective tissue 3e-43

[PIRKW] triple helix 5e-82

[PIRKW] homotπmer 2e-37

[PIRKW] bone 6e-40

[PIRKW] Alport syndrome le-42

[PIRKW] lammin binding 2e-40

[PIRKW] liver 2e-40

[PIRKW] glycoprotein 5e-82

[PIRKW] carboxylic ester hydrolase 7e-24

[PIRKW] disulfide bond 7e-46

[PIRKW] cell binding 7e-46

[PIRKW] heterotrimer 4e-62

[PIRKW] calcium binding 8e-28

[PIRKW] alternative splicing 5e-82

[PIRKW] coiled coil 5e-82

[PIRKW] basement membrane 7e-46

[PIRKW] trimer 5e-82

[PIRKW] pyroglutamic acid 3e-43

[PIRKW] hydroxyprolme 4e-62

[PIRKW] extracellular matrix 5e-82

[PIRKW] chondroit sulfate proteoglycan 6e-41

[PIRKW] sulfoprotein 7e-39

[PIRKW] kidney le-42

[PIRKW] angiogenesis inhibitor 6e-36

[PIRKW] Ehlers-Danlos syndrome 2e-40

[SUPFAM] fibronect type III repeat homology 5e-82

[SUPFAM] scavenger receptor cyste e-rich domain homology le-37

[SUPFAM] C-type lectin homology 6e-30

[SUPFAM] collagen alpha 2(1) chain 5e-40

[SUPFAM] collagen alpha 1(1) chain 6e-44 [SUPFAM] fibrillar collagen carboxyl-terminal homology 6e-44

[SUPFAM] animal Kumtz-type proteinase inhibitor homology 2e-3£

[SUPFAM] fibronect n type II repeat homology 6e-21

[SUPFAM] complement Clq carboxyl-terminal homology le-38

[SUPFAM] collagen alpha 3 (VI) chain 2e-31

[SUPFAM] collagen alpha 1(IV) chain 7e-46

[SUPFAM] collagen alpha 1(VI) chain 2e-37

[SUPFAM] von Willebrand factor type C repeat homology 6e-44

[SUPFAM] unassigned collagens 4e-62

[SUPFAM] von Willebrand factor type A repeat homology 5e-82

[SUPFAM] collagen alpha 1 (XIV) chain 5e-82

[SUPFAM] pulmonary surfactant protein D 6e-30

[SUPFAM] collagen alpha 1 (V) chain 7e-39

[SUPFAM] collagen alpha l(VIII) chain le-38

[SUPFAM] EGF homology le-35

[PROSITE] AMIDATION 3

[PROSITE] MYRISTYL 14

[PROSITE] CK2_PHOSPHO_SITE 13

[PROSITE] PKC_PHOSPHO_SI E 8

[PROSITE] ASN_GLYCOSYLATION 2

[PFAM] von Willebrand factor type A domain

[KW] Irregular

[KW] 3D

[KW] SIGNAL_PEPTIDE 23

[KW] LOW COMPLEXITY 24.24 %

SEQ MAHYITFLCMVLVLLLQNSVLAEDGEVRSSCRTAPTDLVFILDGSYSVGPENFEIVKKWL SEG latzB CCCEEEEEEEECCCCCCHHHHHHHHHHH

SEQ VNITKNFDIGPKFIQVGVVQYSDYPVLEIPLGSYDSGEHLTAAVESILYLGGNTKTGKAI SEG latzB HHHHHHCCBTTTTEEEEEEEETTTEEEEETTTTTTTHHHHHHHHHHCCCCCCCCCHHHHH

SEQ QFALDYLFDKSSRFLTKIAVVLTDGKSQDDVKDAAQAARDSKITLFAIGVGSETEDAELR SEG latzB HHHHHHHHCCTTTTTEEEEEEEECCCTTTTHHHHHHHHHHHCEEEEEEEECCCCCHHHHH

SEQ AIANKPSSTYVFYVEDYIAISKIREVMKQKLCEESVCPTRIPVAARDERGFDILLGLDVN SEG latzB HHHGGGGGGGCECCHHHHHHHHHCHHHHHHHH

SEQ KKVKKRIQLSPKKIKGYEVTSKVDLSELTSNVFPEGLPPSYVFVSTQRFKVKKIWDLWRI SEG latzB

SEQ LTIDGRPQIAVTLNGVDKILLFTTTSVINGSQVVTFANPQVKTLFDEGWHQIRLLVTEQD SEG latzB

SEQ VTLYIDDQQIENKPLHPVLGILINGQTQIGKYSGKEETVQFDVQKLRIYCDPEQNNRETA SEG latzB

SEQ CEI PGFNGECLNGPSDVGSTPAPCICPPGKPGLQGPKGDPGLPGNPGYPGQPGQDGKPGY SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx latzB

SEQ QGIAGTPGVPGSPGIQGARGLPGYKGEPGRDGDKGDRGLPGFPGLHGMPGSKGEMGAKGD SEG xx latzB

SEQ KGSPGFYGKKGAKGEKGNAGFPGLPGPAGEPGRHGKDGLMGSPGFKGEAGSPGAPGQDGT SEG xxxxxxxxxxxxx latzB

SEQ RGEPGIPGFPGNRGLMGQKGEIGPPGQQGKKGAPGMPGLMGSNGSPGQPGTPGSKGSKGE SEG xxxxxxxxxxxxxxxxxxxxxx latzB

SEQ PGIQGMPGASGLKGEPGATGSPGEPGYMGLPGIQGKKGDKGNQGEKGIQGQKGENGRQGI SEG xxxxxxxxxxxxxxxxxxxxx latzB

SEQ PGQQGIQGHHGAKGERGEKGEPGVRGAIGSKGESGVDGLMGPAGPKGQPGDPGPQGPPGL SEG xxxxxxxxxxx xxxxxxxxxxxxxxxxxxxx latzB

SEQ DGKPGREFSEQFIRQVCTDVIRAQLPVLLQSGRIRNCDHCLSQHGSPGIPGPPGPIGPEG SEG xxxxx xxxxxxxxxxxxxxxx latzB

SEQ PRGLPGLPGRDGVPGLVGVPGRPGVRGLKGLPGRNGEKGSQGFGYPGEQGPPGPPGPEGP

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxx latzB

SEQ PGISKEGPPGDPGLPGKDGDHGKPGIQGQPGPPGICDPSLCFSVIARRDPFRKGPNY

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx latzB

Prosite for DKFZphfbr2_2b5.2

PS00001 62->66 ASN_GLYCOΞYLATION PDOC00001 PS00001 329->333 ASN_GLYCOSYLATION PDOC00001 PS00005 30->33 PKC_PHOSPHO_SITE PDOC00005 PS00005 116->119 PKC_PHOSPHO_SITE PDOC00005 PS00005 131->134 PKC_PHOSPHO_SITE PDOC00005 PS00005 250->253 PKC_PHOSPHO_SITE PDOC00005 PS00005 260->263 PKC_PHOSPHO_SITE PDOC00005 PS00005 286->289 PKC_PHOSPHO_SITE PDOC00005 PS00005 393->396 PKC_PHOSPHO_SITE PDOC00005 PS00005 811->814 PKC_PHOSPHO_SITE PDOC00005 PS00006 147->151 CK2_PHOSPHO_SITE PDOC00006 PS00006 172->176 CK2_PHOSPHO_SITE PDOC00006 PS00006 261->265 CK2_PHOSPHO_SITE PDOC00006 PS00006 343->347 CK2_PHOSPHO_SITE PDOC00006 PS00006 357->361 CK2_PHOSPHO_SITE PDOC00006 PS00006 393->397 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 419->423 CK2_PHOSPHO_SITE PDOC00006 PS00006 531->535 CK2_PHOSPHO_SITE PDOC00006 PS00006 600->604 CK2_PHOSPHO_SITE PDOC00006 PS00006 657->661 CK2_PHOΞPHO_SITE PDOC00006 PS00006 681->685 CK2_PHOSPHO_SITE PDOC00006 PS00006 750->754 CK2_PHOSPHO_SITE PDOC00006 PS00006 754->758 CK2_PHOSPHO_SITE PDOC00006 PS00008 92->98 MYRISTYL PDOC00008 PS00008 112->118 MYRISTYL PDOC00008 PS00008 236->242 MYRISTYL PDOC00008 PS00008 276->282 MYRISTYL PDOC00008 PS00008 380->386 MYRISTYL PDOC00008 PS00008 494->500 MYRISTYL PDOC00008 PS00008 527->533 MYRISTYL PDOC00008 PS00008 596->602 MYRISTYL PDOC00008 PS00008 638->644 MYRISTYL PDOC00008 PS00008 650->656 MYRISTYL PDOC00008 PS00008 653->659 MYRISTYL PDOC00008 PS00008 665->671 MYRISTYL PDOC00008 PS00008 743->749 MYRISTYL PDOC00008 PS00008 746->752 MYRISTYL PDOC00008 PS00009 547->551 AMIDATION PDOC00009 PS00009 628->632 AMIDATION PDOC00009 PS00009 694->698 AMIDATION PDOC00009

Pfam for DKFZphfbr2_2b5.2

HMM_NAME von Willebrand factor type A domain

HMM *DIVFLIDGSdSIGpqNFNrMKDFIeRMMERMDIgPDwIRVGVVQYSdNP

D+VF++DGS S+GP NF+++K+ ++++ ++DIGP+ I+VGVVQYSD P

Query 37 DLVFILDGSYSVGPENFEIVKKWLVNITKNFDIGPKFIQVGVVQYSDYP 85

HMM RqEmrFmFNDYQNKeEILQalqqMMyWMgggTNTGeAIQYVvrNMFweer E +++ Y + E++++A+ ++ ++GG T+TG AIQ++++++F +++

Query 86 VLE—IPLGSYDSGEHLTAAVESIL-YLGGNTKTGKAIQFALDYLFDKSS 132

HMM GmRWenvPQVMIIITDGRSQDDIRDpIneMrrmaGIqvFalGIGNhDNnn + +++++++TDG+SQDD++D+++++R+ 1+ FAIG+G

Query 133 RF LTKIAVVLTDGKSQDDVKDAAQAARD-SKITLFAIGVGSETE— 175

HMM WeELRelASePdEdHVFyVdDFeeLdnMqeqL* +ELR IA++P++ +VFYV+D+ +++ ++E +

Query 176 DAELRAIANKPSSTYVFYVEDYIAISKIREVM 207 DKFZphfbr2_2cl

group: brain derived

DKFZphfbr2_2cl encodes a novel 697 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . unknown complete cDNA, complete eds, EST hits

Sequenced by Qiagen

Locus : unknown

Insert length: 3973 bp

Poly A stretch at pos. 3914, polyadenylation signal at pos. 3900

1 GGGGGGATTT CGGCGGCGGA AACATGGCGG TCGCGGCCGG GCCGGTAACG

51 GAGAAAGTTT ACGCCGACAC TGGCCTGTAT TAGCGCGTAT GGCCTCGGGC

101 CCTCGTTCCC CAAGGCGTGC CGCCTCCCTG TTCTCAGTCG CAGGCTGAAG

151 CCTTGTCTGC TCTCCTCCTT TTTGGTTTGG TTTTGGAACT GACTCCGAGG

201 GTTGGGAGAG CGCGTTGGTG GCGACGGCCG AGTCAGATCA CTATAAACAA

251 AATTTCCACA AGAGAAAATG TTGAAATAGG AGTTGCGGAT ACATTGGATA

301 TACTGGATGA AATACAAGCG GTTAATTTTT GTAACGTGAG GGAAAAGCCC

351 ACATTGCTGG TTACATGTGT AAATCACTGC GTTATTGCTT TAGTCATTGT

401 CTCTATTTAG CAATGACAAG ACTGGAAGAA GTAAATAGAG AAGTGAACAT

451 GCATTCTTCA GTGCGGTATC TTGGCTATTT AGCCAGAATC AATTTATTGG

501 TTGCTATATG CTTAGGTCTA TACGTAAGAT GGGAAAAAAC AGCAAATTCC

551 TTAATTTTGG TAATTTTTAT TCTTGGTCTT TTTGTTCTTG GAATCGCCAG

601 CATACTCTAT TACTATTTTT CAATGGAAGC AGCAAGTTTA AGTCTCTCCA

651 ATCTTTGGTT TGGATTCTTG CTTGGCCTCC TATGTTTTCT TGATAATTCA

701 TCCTTTAAAA ATGATGTAAA AGAAGAATCA ACCAAATATT TGCTTCTAAC

751 ATCCATAGTG TTAAGGATAT TGTGCTCTCT GGTGGAGAGA ATTTCTGGCT

801 ATGTCCGTCA TCGGCCCACT TTACTAACCA CAGTTGAATT TCTGGAGCTT

851 GTTGGATTTG CCATTGCCAG CACAACTATG TTGGTGGAGA AGTCTCTGAG

901 TGTCATTTTG CTTGTTGTAG CTCTGGCTAT GCTGATTATT GATCTGAGAA

951 TGAAATCTTT CTTAGCTATT CCAAACTTAG TTATTTTTGC AGTTTTGTTA

1001 TTTTTTTCCT CATTGGAAAC TCCCAAAAAT CCGATTGCTT TTGCGTGTTT

1051 TTTTATTTGC CTGATAACTG ATCCTTTCCT TGACATTTAT TTTAGTGGAC

1101 TTTCAGTAAC TGAAAGATGG AAACCCTTTT TGTACCGTGG AAGAATTTGC

1151 AGAAGACTTT CAGTCGTTTT TGCTGGAATG ATTGAGCTTA CATTTTTTAT

1201 TCTTTCCGCA TTCAAACTTA GAGACACTCA CCTCTGGTAT TTTGTAATAC

1251 CTGGCTTTTC CATTTTTGGA ATTTTCAGGA TGATTTGTCA TATTATTTTT

1301 CTTTTAACTC TTTGGGGATT CCATACCAAA TTAAATGACT GCCATAAAGT

1351 ATATTTTACT CACAGGACAG ATTACAATAG CCTTGATAGA ATCATGGCAT

1401 CCAAAGGGAT GCGCCATTTT TGCTTGATTT CAGAGCAGTT GGTGTTCTTT

1451 AGTCTTCTTG CAACAGCGAT TTTGGGAGCA GTTTCCTGGC AGCCAACAAA

1501 TGGAATTTTC TTGAGCATGT TCCTAATCGT TTTGCCATTG GAATCCATGG

1551 CTCATGGGCT CTTCCATGAA TTGGGTAACT GTTTAGGAGG AACATCTGTT

1601 GGATATGCTA TTGTGATTCC CACCAACTTC TGCAGTCCTG ATGGTCAGCC

1651 AACACTGCTT CCCCCAGAAC ATGTACAGGA GTTAAATTTG AGGTCTACTG

1701 GCATGCTCAA TGCTATCCAA AGATTTTTTG CATATCATAT GATTGAGACC

1751 TATGGATGTG ACTATTCCAC AAGTGGACTG TCATTTGATA CTCTGCATTC

1801 CAAACTAAAA GCTTTCCTCG AACTTCGGAC AGTGGATGGA CCCAGACATG

1851 ATACGTATAT TTTGTATTAC AGTGGGCACA CCCATGGTAC AGGAGAGTGG

1901 GCTCTAGCAG GTGGAGATAC ACTACGCCTT GACACACTTA TAGAATGGTG

1951 GAGAGAAAAG AATGGTTCCT TTTGTTCCCG GCTTATTATC GTATTAGACA

2001 GCGAAAATTC AACCCCTTGG GTGAAAGAAG TGAGGAAAAT TAATGACCAG

2051 TATATTGCAG TGCAAGGAGC AGAGTTGATA AAAACAGTAG ATATTGAAGA

2101 AGCTGACCCG CCACAGCTAG GTGACTTTAC AAAAGACTGG GTAGAATATA

2151 ACTGCAACTC CTGTAATAAC ATCTGCTGGA CTGAAAAGGG ACGCACAGTG

2201 AAAGCAGTAT ATGGTGTGTC AAAACGGTGG AGTGACTACA CTCTGCATTT

2251 GCCAACGGGA AGCGATGTGG CCAAGCACTG GATGTTACAC TTTCCTCGTA

2301 TTACATATCC CCTAGTGCAT TTGGCAAATT GGTTATGCGG TCTGAACCTT

2351 TTTTGGATCT GCAAAACTTG TTTTAGGTGC TTGAAAAGAT TAAAAATGAG

2401 TTGGTTTCTT CCTACTGTGC TGGACACAGG ACAAGGCTTC AAACTTGTCA

2451 AATCTTAATT TGGACCCCAA AGCGGGATAT TAATAAGCAC TCATACTACC

2501 AATTATCACT AACTTGCCAT TTTTTGTATG CTGTATTTTT ATTTGTGGAA

2551 AATACCTTGC TACTTCTGTA GCTGCTCTCA CTTTGTCTTT TCTTAAGTAA

2601 TTATGGTATA TATAAGGCGT TGGGAAAAAA CATTTTATAA TGAAAGTATG

2651 TAGGGAGTCA AATGCTTACT GTAAATGCAT AAGAGACGTT AAAAATAACA

2701 CTGCACTTTC AGGAATGTTT GCTTATGGTC CTGATTAGAA AGAAACAGTT 2751 GTCTATGCTC TGCAATGGTC AATGATGAAT TACTAATGCC TTATTTTCTA

2801 GGCATATAAT AATAGTTTAG AGAATGTAGA CCAGATAAAT TTGTTTACTG

2851 TTTTAAGAAA ACTACCAGTT TACTTACAGA AGATTCTTTT TTCCAAACAG

2901 TAGGTTTCAT CCAAGACCAT TTGAAGAACT GCAAACTCTT TCTCTTAGAA

2951 AAGAAAGAGG GCAGCCTAAA ATAAACGCAA AATTTGCTTA TACTCCATCA

3001 CATTCAGATG TCTTGGTTGT GACTTATTAC CAGTGTGGCA GAGAACCCAA

3051 GTTACATTTT AGATCAAAAT ATTCTTTATG TAGGTATTGT TAAAAGGCTA

3101 GAGCCTACAA GTTGCTCTTC CATGCGTTGG TCAGGGGGCC CTGAAAACAC

3151 TGGTAATATT AAGAGTCTTT CTCAGGGTAA CTTAATGTTT TCTTAATGAA

3201 CAGTGTTTCC AGCTACAAAT TCTTCCAATA AATTGTCTTC CTTTTTGAAA

3251 AGTACTCTCA TAGAAGAAAT TTAGCAATTT CTCGTTGACT GACTCAGTCT

3301 ATTTTAAGTA TTCAGAAAAG ATTTTGATCC CCATTGAGTT AATGCTCTGC

3351 CTTGAAAATT ATTTTTCTGA TCCTTGTTAG TGATAACATT TTTTTTCTAC

3401 TGAAGGTCAG AGGATAGGAA ACAAGTATTT CTCTTCTGGT ATACATGTAA

3451 TGTATTCTGT AAAAAAGTAT TCATATTGGC AATTTTAGTT AGGCATAATA

3501 TTGTGGTTGT AATTTTTAAA ACTTAGTGTT TTGTCTGATT AAAGCAGGCA

3551 CTGATCAGGG TATCTCCTAA GAGGTAATTC ACTTCTTATT CCTTTCCAAT

3601 AATTATTACA TTCTAAATTT TCATCTATGA GAAATAACAA ACAAGAAGGG

3651 AATAGAATTA AATTGGGGTA TAATCTAATC TTCATTGTTT AAATGGTTTG

3701 CCTTCTCACC ATTGAAGCCA TTTTTTTATA GCCTCAGAAA GAGGAAATAA

3751 TGCCTCCACC ATTTTCTACC TGGTGACTTG AAAATTGAAC TTTTAAGTTA

3801 GGAAGAAGTT AGAGTCAGGG AACTTGTATA CCACTATCTA TGCAGCATTG

3851 TTATAGTCTG ATTATTTCTG TGTTTTGAAT ATGATTTTCC TAATGCTCTA

3901 AATAAAATTT TGTTAAAAAT CAAAAAAAAA AAAAAAAAAA CTTATCGATA

3951 CCGTCGACCT CGATGATGTC GAC

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 365 bp to 2455 bp; peptide length: 697 Category: putative protein Classification: unset

1 MCKSLRYCFS HCLYLAMTRL EEVNREVNMH SSVRYLGYLA RINLLVAICL 51 GLYVRWEKTA NSLILVIFIL GLFVLGIASI LYYYFSMEAA ΞLSLSNLWFG 101 FLLGLLCFLD NSSFKNDVKE ESTKYLLLTS IVLRILCSLV ERISGYVRHR 151 PTLLTTVEFL ELVGFAIAST TMLVEKSLSV ILLVVALAML IIDLRMKSFL 201 AIPNLVIFAV LLFFSSLETP KNPIAFACFF ICLITDPFLD IYFSGLSVTE 251 RWKPFLYRGR ICRRLSVVFA GMIELTFFIL ΞAFKLRDTHL WYFVIPGFSI 301 FGIFRMICHI IFLLTLWGFH TKLNDCHKVY FTHRTDYNSL DRIMASKGMR 351 HFCLISEQLV FFSLLATAIL GAVSWQPTNG IFLSMFLIVL PLESMAHGLF 401 HELGNCLGGT SVGYAIVIPT NFCSPDGQPT LLPPEHVQEL NLRSTGMLNA 451 IQRFFAYHMI ETYGCDYSTS GLSFDTLHΞK LKAFLELRTV DGPRHDTYIL 501 YYSGHTHGTG EWALAGGDTL RLDTLIEWWR EKNGSFCSRL IIVLDSENST 551 PWVKEVRKIN DQYIAVQGAE LIKTVDIEEA DPPQLGDFTK DWVEYNCNSC 601 NNICWTEKGR TVKAVYGVSK RWSDYTLHLP TGSDVAKHWM LHFPRITYPL 651 VHLANWLCGL NLFWICKTCF RCLKRLKMSW FLPTVLDTGQ GFKLVKS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_2cl, frame 2

PIR:A71148 hypothetical protein PH0395 - Pyrococcus horikoshn, N = 1, Score = 96, P = 0.12

>PIR:A71148 hypothetical protein PH0395 Pyrococcus horikoshn Length = 288

HSPs:

Score = 96 (14.4 bits), Expect = 1.3e-01, P = 1.2e-01 Identities = 59/234 (25%), Positives = 116/234 (49%) Query: 77 IASILYYYFSMEAASLSLSNLWFGFLL--GL—LCFLDNSSFKNDVKEESTKYLLLTΞIV 132

++ +LYY F+ A ++ L G+LL + L +L N + V+ + K + ++ Sbjct: 57 LSLVLYYLFAFSALK-TIIFLALGYLLMNSIYELGYLMNDTISRRVEGKVHKVRVKLTVF 115

Query: 133 LRILCSLVERISGYVRHRPTLLTTVEFLELVGFAIASTTMLVEKSLSVILLVVALAMLII 192

+L +L I YV ++ T+ FL+LVG ++ +L E +L ++ L+ L + Sbjct: 116 DSLLIALSRAI--YV VIFTLVFLKLVGLQYSTQVILAEVTLFLVFLLYDLTPKHV 168

Query: 193 DLRMKSFLAIPNLVIFAVLLFFSSLET-PKNPIAFACFFICLITDPFLDIYFSGLSVTER 251

M SF + + F +LL F T +N I + FI I F ++ + + Sbjct: 169 RTVMLSF-PLKFMKAFVLLLPFIITGTLVENVITLΞ—FILPIAVRFSQAHYLKTACKDN 225

Query: 252 WKPFLYRGRICRRLSVVFAGMIEL-TFFILSAFK-LRDTHLW-YFVIPGFSIFGIFRMIC 308

P ++ R+ R S+++ + L TF +L +F L +T L ++IP F++ + ++ Sbjct: 226 -PPRDFKRRV-ERFSMMYLQVTSLSTFTVLVSFVYLGNTDLLRQYLIP-FAVNVVLILLΞ 282

Query: 309 HI 310

++ Sbjct: 283 YL 284

Pedant information for DKFZphfbr2_2cl, frame 2

Report for DKFZphfbr2_2cl .2

[LENGTH] 697

[MW] 79741.46

[pl] 8.41

[KW] TRANSMEMBRANE 11

[KW] LOW COMPLEXITY 9.76 %

SEQ MCKSLRYCFSHCLYLAMTRLEEVNREVNMHΞΞVRYLGYLARINLLVAICLGLYVRWEKTA

SEG PRD ccceeehhhhhhhhhhhhhhhhhhhhhhccceeeehhhhhhhhhhhhhhhhhhhcccccc MEM MMMMMMMMMMMMMMMMM

SEQ NSLILVIFILGLFVLGIASILYYYFSMEAASLSLSNLWFGFLLGLLCFLDNSSFKNDVKE SEG ..xxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxx PRD ccceeeeccccchhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccc MEM ...MMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMM

SEQ ESTKYLLLTSIVLRILCSLVERISGYVRHRPTLLTTVEFLELVGFAIASTTMLVEKSLSV SEG xxxxxxxxxxxx xxxx PRD ccchhhhhhhhhhhhhhhhhhhceeeeccccccchhhhhhhhhhhhhhhhhhhhhhhhhh MEM ....MMMMMMMMMMMMMMMMM MMM

SEQ ILLVVALAMLIIDLRMKSFLAIPNLVIFAVLLFFSSLETPKNPIAFACFFICLITDPFLD SEG xxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhcccccccccchhhhhhhhhcccccee MEM MMMMMMMMMMMMMM ...MMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMM.

SEQ IYFSGLSVTERWKPFLYRGRICRRLSVVFAGMIELTFFILSAFKLRDTHLWYFVIPGFSI SEG PRD eeeccccccccccceeecccccccchhhhhhhhhhhhhhhhhhhccccceeeeeeccccc MEM MMMMMMMMMMMMMMMMM M

SEQ FGIFRMICHIIFLLTLWGFHTKLNDCHKVYFTHRTDYNSLDRIMASKGMRHFCLISEQLV SEG PRD hhhhhhhhhhhhhhhhhcccccccceeeeeeeccccccchhhhhhhcccchhhhhhhhhh MEM MMMMMMMMMMMMMMMM MM

SEQ FFSLLATAILGAVSWQPTNGIFLSMFLIVLPLESMAHGLFHELGNCLGGTSVGYAIVIPT SEG PRD hhhhhhhhhhhhcccccccchhhhhhhheeehhhhhhhhhhccccccccccceeeeeeec MEM MMMMMMMMMMMMMMM ....MMMMMMMMMMMMMMMMM

SEQ NFCSPDGQPTLLPPEHVQELNLRSTGMLNAIQRFFAYHMIETYGCDYSTSGLSFDTLHSK SEG PRD ccccccccccccccccccccccccchhhhhhhhhhhhhhhhccccccccccccchhhhhh MEM

SEQ LKAFLELRTVDGPRHDTYILYYSGHTHGTGEWALAGGDTLRLDTLIEWWREKNGSFCSRL SEG

PRD hhhhhhhhhccccccceeeeeeccccccccceeeccccchhhhhhhhhhhhccccceeee MEM

SEQ IIVLDSENSTPWVKEVRKINDQYIAVQGAELIKTVDIEEADPPQLGDFTKDWVEYNCNSC SEG

PRD eeeeecccccccchhhhhhccceeeeccceeeeeeeecccccccccccccceeeeccccc

MEM

SEQ NNICWTEKGRTVKAVYGVSKRWSDYTLHLPTGSDVAKHWMLHFPRITYPLVHLANWLCGL

SEG

PRD cceeeecccceeeeeeeecccccceeeecccccchhhhhhhcccccccchhhhhhhhhcc

MEM

SEQ NLFWICKTCFRCLKRLKMSWFLPTVLDTGQGFKLVKS

SEG

PRD eeeeeehhhhhhhhhhhhhhcceeeeccccccccccc

MEM

(No Prosite data available for DKFZphfbr2_2cl .2) (No Pfam data available for DKFZphfbr2_2cl .2)

DKFZphfbr2 2cl7

group: signal transduction

DKFZphfbr2_₂cl7.3 encodes a novel 446 amino ac d protein with similarity to yeast YMR131C and mammalian retinoblastoma-binding protein RbAp46

The protein contains 1 WD-40 repeat, which is typical for the beta-transducm subunit of G- proteins . The beta subunits seem to be required for the replacement of GDP by GTP as well as for membrane anchoring and receptor recognition.

The new protein can find application m modulating/blocking G-protein-dependent pathways. similarity to YMR131C and retinoblastoma-bmding protein RbAp46 complete cDNA, complete eds, EST hits

Sequenced by Qiagen

Locus: unknown

Insert length: 2248 bp

Poly A stretch at pos. 2230, polyadenylation signal at pos. 2200

1 TGGGGAAGAT GGCGGCGCGC AAGGGTCGGC GTCGCACGTG TGAAACCGGG

51 GAACCCATGG AAGCCGAGTC CGGCGACACA AGTTCCGAGG GCCCGGCCCA

101 GGTCTACCTG CCCGGCCGGG GGCCGCCGCT ACGCGAAGGG GAGGAGCTGG

151 TCATGGACGA GGAGGCCTAT GTGCTCTACC ACCGAGCGCA GACTGGCGCC

201 CCCTGTCTCA GCTTTGACAT AGTCCGGGAT CACCTGGGAG ACAACCGGAC

251 AGAGCTTCCT CTTACACTTT ACTTGTGTGC TGGGACCCAG GCTGAGAGCG

301 CCCAGAGCAA CAGACTGATG ATGCTTCGGA TGCACAATCT GCATGGGACA

351 AAGCCCCCAC CCTCAGAGGG CAGTGATGAA GAAGAAGAGG AGGAAGATGA

401 AGAGGATGAA GAAGAGCGGA AACCTCAGCT GGAGCTGGCC ATGGTGCCCC

451 ACTATGGTGG CATCAACCGA GTTCGGGTGT CATGGCTGGG TGAAGAGCCT

501 GTGGCTGGGG TGTGGTCAGA GAAGGGCCAG GTGGAGGTGT TTGCGCTGCG

551 GCGGCTTCTG CAGGTGGTGG AGGAGCCCCA GGCCCTGGCA GCCTTCCTCC

601 GGGATGAGCA GGCCCAAATG AAGCCCATCT TCTCCTTCGC TGGACACATG

651 GGCGAGGGCT TTGCCCTTGA CTGGTCCCCC CGGGTGACCG GTCGCCTGCT

701 GACCGGTGAC TGTCAAAAGA ACATCCACCT CTGGACACCT ACGGACGGCG

751 GCTCCTGGCA CGTGGACCAG CGGCCATTCG TGGGCCACAC ACGCTCTGTG

801 GAGGACCTGC AGTGGTCACC GACTGAGAAC ACGGTGTTTG CCTCCTGCTC

851 AGCTGACGCC TCCATCCGCA TCTGGGACAT CCGGGCAGCC CCCAGCAAGG

901 CCTGCATGCT CACCACAGTC ACCGCCCATG ATGGGGACGT CAATGTCATC

951 AGCTGGAGCC GCCGGGAGCC CTTCCTGCTC AGTGGCGGGG ATGATGGGGC

1001 CCTCAAGATC TGGGACCTTC GGCAGTTCAA GTCTGGTTCC CCAGTGGCCA

1051 CCTTCAAGCA GCACGTGGCC CCCGTGACCT CCGTCGAGTG GCACCCCCAG

1101 GACAGCGGGG TCTTTGCAGC CTCGGGTGCA GACCACCAGA TCACACAGTG

1151 GGACCTGGCA GTGGAGCGGG ACCCTGAGGC GGGCGACGTG GAGGCCGACC

1201 CCGGACTGGC CGACCTCCCG CAGCAGCTGC TGTTCGTGCA CCAGGGCGAG

1251 ACCGAGCTGA AGGAGCTGCA CTGGCACCCG CAGTGCCCAG GGCTCCTGGT

1301 CAGCACGGCG CTGTCAGGCT TCACCATCTT CCGCACCATC AGCGTCTGAG

1351 GCGTCCCACT GGCTCTGATC TTGCTTCCTG CTTGGAAACT GAAGTCGAAT

1401 TGGGCTCCCC TGGAAGGGGT TCATTCAGGT CTGTTGACTG AGACTGGCCG

1451 GCCTGTGGGC TGCCGTGATG GATTCTGTTT GACGTATTGT TCTCTAGAAG

1501 GCCTGGCTCT GATCCAGTGA CCCCTCTCAC CAAAGAACTC GGTTTAACCA

1551 GGGCTCTGTA AGACCACTCC CACCCAGAGA CTTGTGTGGC CTGGTGTGGC

1601 CTGTGTGTCG GATTCCTTCC TGTCAGCTGT GACCCATTTG ACCTGTGTCC

1651 CCAGAACCCA GTTTTTTGTT TGTTTGTTTG AGACGGAGTC TTGGTCTGTC

1701 GCCCAGGCTG GAGTGCAGTA GCACGATCTT GGCTCACTGC AACCTCCGCC

1751 TCCTGGGTTA AAGTGATTCT CTCAGCTCAG TCTCCCAGGT AGCTGGGATT

1801 ACAGGCATGT GCCACCACAC CCCGTTAATT TTTGTATTTT TAGTAGAGAC

1851 GGGGTTTCAC CATGTTGGCC AGGCTGGTCT CAAATTCTTG ATCTCAAGTG

1901 ATCTGTCCGC CCCGGCCTCC CAGAGTGCTG GGTTGGGATT ACAGGCGTGA

1951 GCCACCGCGT CCGGCTCAGG ACCCAGTTTT GGCTGCTGGT TCCCAGCAGG

2001 GGACTCGGGG GATATACAGT GGCTGCACCA AATTGGAGGT GTGGGTTCCT

2051 CCAACACAAT TTGCTTCTGC CCGTTGTCTT CCTGCCAGCT GGGTTTGGCC

2101 AGGATTTCTC CGTGTGGGGG CTACATGCGA CCCTCTCCCC TCCTCCCTGA

2151 CTTTAGAGGC TGGTGCTGTG TCGGGAGGAA GGTCAGGGCT CCTGAGCAGC

2201 AATAAAGGAC CAGGAAGAGG CCTGAGGTGG AAAAAAAAAA AAAAAAAA

BLAST Results o BLAST result Medlme entries

No Medline entry

Peptide information for frame 3

ORF from 9 bp to 1346 bp; peptide length: 446 Category: similarity to known protein Classification: unset Prosite motifs: WD REPEATS (323-338)

1 MAARKGRRRT CETGEPMEAE SGDTSSEGPA QVYLPGRGPP LREGEELVMD

51 EEAYVLYHRA QTGAPCLSFD I RDHLGDNR TELPLTLYLC AGTQAESAQS

101 NRLMMLRMHN LHGTKPPPSE GSDEEEEEED EEDEEERKPQ LELAMVPHYG

151 GINRVRVSWL GEEPVAGVWS EKGQVEVFAL RRLLQVVEEP QALAAFLRDE

201 QAQMKPIFSF AGHMGEGFAL DWSPRVTGRL LTGDCQKNIH LWTPTDGGSW

251 HVDQRPFVGH TRSVEDLQWS PTENTVFASC SADASIRIWD IRAAPSKACM

301 LTTVTAHDGD VNVISWSRRE PFLLSGGDDG ALKIWDLRQF KSGSPVATFK

351 QHVAPVTSVE WHPQDSGVFA ASGADHQITQ WDLAVERDPE AGDVEADPGL

401 ADLPQQLLFV HQGETELKEL HWHPQCPGLL VSTALSGFTI FRTISV

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_2cl7, frame 3

TREMBL:AC005917_14 gene: "F3P11.14"; product: "putative WD-40 repeat protein"; Arabidopsis thaliana chromosome II BAC F3P11 genomic sequence, complete sequence., N = 1, Score = 910, P = 2.7e-91

PIR:S53061 hypothetical protein YMR131c - yeast (Saccharomyces cerevisiae), N = 1, Score = 691, P = 4.3e-68

PIR: 149367 retinoblastoma-bindmg protein mRbAp46 - mouse, N = 1, Score = 338, P = l.le-30

PIR: 139181 retinoblastoma-bindmg protein RbAp46 - human, N = 1, Score = 338, P = l.le-30

>TREMBL:AC005917_14 gene: "F3P11.14"; product: "putative WD-40 repeat protein"; Arabidopsis thaliana chromosome II BAC F3P11 genomic sequence, complete sequence. Length = 469

HSPs:

Score = 910 (136.5 bits), Expect = 2.7e-91, P = 2.7e-91 Identities = 195/442 (44%), Positives = 259/442 (58%)

Query: 18 EAESGDTSSEGPAQVYLPGRGPPLREGEELVMDEEAYVLYHRAQTGAPCLSFDIVRDHLG 77

EA S + S P +V+ PG L +GEEL D AY H G PCLSFDI+ D LG Sbjct: 18 EASSSEIPSI-PTRVWQPGVDT-LEDGEELQCDPSAYNSLHGFHVGWPCLΞFDILGDKLG 75

Query: 78 DNRTELPLTLYLCAGTQAESAQSNRLMMLRMHNLHGTKP PPSEGSDEEEEEEDEED- 133

NRTE P TLY+ AGTQAE A N + + ++ N+ G + P + G+ E+E+E+DE+D Sbjct: 76 LNRTEFPHTLYMVAGTQAEKAAHNSIGLFKITNVSGKRRDVVPKTFGNGEDEDEDDEDDS 135

Query: 134 EEERKPQLELAMVPHYGGINRVRVSWLGEEPVAGVWSEKGQVEVFALRRLLQ 185

E + P +++ V H+G +NR+R + W++ G V+V+ + L Sbjct: 136 DSDDDDGDEASKTPNIQVRRVAHHGCVNRIRAMPQNSH-ICVSWADSGHVQVWDMSSHLN 194

Query: 186 VVEEPQALAAFLRDEQAQMKPIFSFAGHMGEGFALDWSPRVTGRLLTGDCQKNIHLWTPT 245

+ E + P+ +F+GH EG+A+DWSP GRLL+GDC+ IHLW P

Sbjct: 195 ALAESETEGKDGTSPVLNQAPLVNFSGHKDEGYAIDWSPATAGRLLΞGDCKSMIHLWEPA 254

Query: 246 DGGSWHVDQRPFVGHTRSVEDLQWSPTENTVFASCSADASIRIWDIRAAPSKACMLTTVT 305

G SW VD PF GHT SVEDLQWSP E VFASCS D S+ +WDIR Ξ A + Sbjct: 255 SG-SWAVDPIPFAGHTASVEDLQWSPAEENVFASCSVDGSVAVWDIRLGKSPAL SFK 310

Query: 306 AHDGDVNVISWSRREPFLL-SGGDDGALKIWDLRQFKSGSPV-ATFKQHVAPVTSVEWHP 363

AH+ DVNVISW+R +L SG DDG I DLR K G V A F+ H P+TS+EW Sbjct: 311 AHNADVNVISWNRLASCMLASGSDDGTFSIRDLRLIKGGDAVVAHFEYHKHPITSIEWSA 370 Query: 364 QDSGVFAASGADHQITQWDLAVERDPE AGDVEADPGLADLPQQLLFVHQGETEL 417

++ A + D+Q+T WDL++E+D E A E DLP QLLFVHQG+ +L Sbjct: 371 HEASTLAVTSGDNQLTIWDLSLEKDEEEEAEFNAQTKELVNTPQDLPPQLLFVHQGQKDL 430

Query: 418 KELHWHPQCPGLLVSTALSGFTIFRTISV 446

KELHWH Q PG+++STA GF I ++ Sbjct: 431 KELHWHNQIPGMIISTAGDGFNILMPYNI 459

Pedant information for DKFZphfbr2_2cl7, frame 3

Report for DKFZphfbr2_2cl7.3

[LENGTH] 446 [MW] 49447.38 [pi] 4.82 [HOMOL] TREMBL:AC005917_14 gene: "F3P11.14"; product: "putative WD-40 repeat protein" Arabidopsis thaliana chromosome II BAC F3P11 genomic sequence, complete sequence, le-90 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YMR131c] 4e-65 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YEL056w] 4e-15 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YEL056w] 4e-15 [FUNCAT] 06.07 protein modification (glycolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YEL056w] 4e-15 [FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YBR195c] 2e-13 [FUNCAT] 10.04.09 regulation of g-protein activity [S. cerevisiae, YBR195c] 2e-13 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YBR195C] 2e-13 [ FUNCAT ] 03.16 dna synthesis and replication [S. cerevisiae, YBR195c] 2e-13 [ FUNCAT ] 09.13 biogenesis of chromosome structure [S. cerevisiae, YBR195c] 2e-13 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YPR178w] le-11 [FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YPR178w] le-11 [FUNCAT] 06.13 proteolysis [S. cerevisiae, YGL003c] 4e-09 [ FUNCAT ] 03.22 cell cycle control and mitosis [S. cerevisiae, YGL003c] 4e-09 [FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YDL145c] 5e ^■09 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL145c] 5e-09 [FUNCAT] 04.05.01.01 general transcription activities [S. cerevisiae, YBR198c TAF90 - TFIID subunit] 6e-09 [FUNCAT] 05.04 translation (initiation, elongation and termination) [S cerevisiae, YMR116C] 5e ^■08 [FUNCAT] 02.16 fermentation [S. cerevisiae, YMR116c] 5e-08 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YLR429w] 3e-07 [FUNCAT] 30.19 peroxisomal organization [S. cerevisiae, YDR142c] 3e-06 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YDR142c] 3e-06 [FUNCAT] 08.10 peroxisomal transport [S. cerevisiae, YDR142c] 3e-06 [FUNCAT] 03.13 meiosis [S. cerevisiae, YLR129w] 4e-06 [FUNCAT] 08.01 nuclear transport [S. cerevisiae, YER107c] 4e-06 [FUNCAT] 03.01 cell growth [Ξ. cerevisiae, YKL021C] 4e-06 [FUNCAT] 04.07 rna transport [S. cerevisiae, YER107c] 4e-06 [FUNCAT] 03.25 cytokinesis [Ξ. cerevisiae, YCR057c] 2e-05 (FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YCR057c] 2e-05 [FUNCAT] 01.01.04 regulation of amino-acid metabolism [S. cerevisiae, YIL046w] 2e-05 [FUNCAT] 06.13.01 cytoplasmic degradation [S. cerevisiae, YIL046w] 2e-05 [FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YLLOllw] 3e-05 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YOR212w] 5e-05 [FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins

[S. cerevisiae, YOR212w] 5e-05 [FUNCAT] 10.05.07 g-proteins [S. cerevisiae, YOR212w] 5e-05 [BLOCKS] BL00678 [SCOP] d2trcb_ 2 . 51. 3. 1. 1 Transducin (heterotrimeπc G protein) , gamm 5e-29 [PIRKW] plasma 6e-07 [PIRKW] duplication 4e-12 [PIRKW] hormone 6e-07 [PIRKW] transmembrane protein le-07 [PIRKW] stomach 6e-07 [PIRKW] actin binding le-07 [PIRKW] leucine zipper le-07 [PIRKW] signal transduction 2e-06 [PIRKW] heterotrimer 2e-06 [PIRKW] peripheral membrane protein 6e-07 [PIRKW] GTP binding 2e-06 [SUPFAM] WD repeat homology le-63 [SUPFAM] yeast coatomer complex alpha chain le-07 [SUPFAM] GTP-binding regulatory protein beta chain 4e-07 [SUPFAM] PRL1 protein 8e-09 [ SUPFAM] MSI 1 protein 4e- 12

[ SUPFAM] coatomer complex beta ' chain le-09

[ PROSITE] WD_REPEATS 1

[ PFAM] WD domain , G-beta repeats

[ KW] All_Beta

[ KW] 3D

[KW] LOW COMPLEXITY 3.14 %

SEQ MAARKGRRRTCETGEPMEAESGDTSSEGPAQVYLPGRGPPLREGEELVMDEEAYVLYHRA SEG IgotB

SEQ QTGAPCLSFDIVRDHLGDNRTELPLTLYLCAGTQAESAQSNRLMMLRMHNLHGTKPPPSE SEG IgotB

SEQ GSDEEEEEEDEEDEEERKPQLELAMVPHYGGINRVRVSWLGEEPVAGVWSEKGQVEVFAL SEG .. xxxxxxxxxxxxxx IgotB

SEQ RRLLQVVEEPQALAAFLRDEQAQMKPIFSFAGHMGEGFALDWSPRVTGRLLTGDCQKNIH SEG IgotB EEECCCCCEEEEEETTT-TCEEEEEETTTEEE

SEQ LWTPTDGGSWHVDQRPFVGHTRSVEDLQWSPTENTVFASCSADASIRIWDIRAAPSKACM SEG IgotB EEETTTT CEEEEEECCCCCEEEEEEETTTCE-EEEEETTTEEEEEETTT—TEEEE

SEQ LTTVTAHDGDVNVISWSRREPFLLSGGDDGALKIWDLRQFKSGSPVATFKQHVAPVTSVE SEG IgotB EECBTTBTCCEEEEEETTTTTEEEEEETTTEEEEEE

SEQ WHPQDSGVFAASGADHQITQWDLAVERDPEAGDVEADPGLADLPQQLLFVHQGETELKEL SEG IgotB

SEQ HWHPQCPGLLVSTALSGFTIFRTISV SEG IgotB

Prosite for DKFZphfbr2_2cl7.3

PS00678 323->338 WD REPEATS PDOC00574

Pfam for DKFZphfbr2_2cl7.3

HMM_NAME WD domain, G-beta repeats

HMM *MrGHnnWVWCVaFSPDGrWFIvSGSWDgTCRLWD* ++GH+ V ++ +SP + +++S S D ++R+WD

Query 257 FVGHTRSVEDLQWSPTENTVFASCSADASIRIWD 290

24.88 304 336 1 34 dkfzphfbr2_2cl7.3 similarity to YMR131c and retinoblastoma- bindmg protein RbAp46

Alignment to HMM consensus: Query *MrGHnnWVWCVaFSPDGrWFIvSGSWDgTCRLWD*

+ H+++V+ +++S + ++SG++DG +++WD dkfzphfbr2 304 VTAHDGDVNVISWSRREPF-LLΞGGDDGALKIWD 336 DKFZphfbr2_2cl8

group: brain associated

DKFZphfbr2_2cl8 encodes a novel 302 ammo acid protein with weak similarity to cyclin- dependent kinase pl30-PITSLRE.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . weak similarity to cyclin-dependent kinase pl30-PITΞLRE complete cDNA, complete eds, EST hits

Sequenced by Qiagen

Locus: unknown

Insert length: 2835 bp

Poly A stretch at pos. 2817, polyadenylation signal at pos. 2796

1 TGGGGCGGAC GGCGAGGGAG TCCAGAGCCT TGAGCCCGGT GCTCCTCCCT 51 CGCGCAGCGG TGGCTCTGCG GCCGCTGGAG TAAACACTGC CTTTGTTCCC

101 TAGCGCCTCG TCTTTCGTCG CCCCGTGCCC TCACGCCGCC GGGCTCTGGC

151 CGGCCCGCCC TCGGTCCTTG AACCCCATTT CGGCTCGTGC CGTGCGGATG

201 CAGCTGCCGG GCCTGGGTTT GGGCATTGAG CGGGAGGAGG AGGAGGAGCG

251 GCGGCGCCTG GGCGGCATGC GATGGGGAAC TGCTGCTGGA CGCAGTGCTT

301 CGGACTGCTT CGCAAGGAAG CGGGGCGGCT GCAGCGAGTA GGCGGCGGCG

351 GAGGATCCAA GTATTTTAGA ACATGCTCAA GAGGTGAGCA CTTGACAATA

401 GAGTTTGAGA ATCTAGTAGA AAGTGATGAA GGGGAGAGCC CAGGAAGCAG

451 TCATAGGCCT CTTACTGAGG AAGAAATTGT TGACCTAAGA GAAAGGCATT

501 ATGATTCCAT TGCCGAAAAA CAAAAAGATC TTGATGAGAA AATTCAAAAA

551 GAGTTAGCCT TACAAGAAGA GAAGTTAAGA CTAGAAGAAG AAGCTTTATA

601 CGCTGCACAG CGTGAAGCAG CCAGGGCAGC AAAGCAGCGA AAGCTCTTGG

651 AGCAAGAAAG GCAGAGAATT GTGCAGCAAT ATCATCCTTC CAACAATGGA

701 GAATATCAAA GTTCAGGACC AGAAGATGAC TTCGAATCTT GTTTGAGAAA

751 TATGAAGTCA CAGTATGAAG TTTTTCGAAG TAGTAGACTC TCATCAGATG

801 CTACAGTTTT GACACCAAAT ACAGAAAGCA GTTGTGATTT AATGACCAAA

851 ACTAAATCAA CTAGTGGAAA TGACGACAGC ACATCCTTAG ATCTAGAGTG

901 GGAAGATGAA GAAGGAATGA ATAGAATGCT TCCAATGAGA GAACGTTCCA

951 AAACAGAGGA AGACATTCTA CGGGCAGCAC TTAAGTATAG CAACAAGAAG 1001 ACTGGAAGTA ATCCTACATC AGCCTCTGAT GATTCCAATG GGCTGGAGTG 1051 GGAAAATGAT TTTGTTAGTG CCGAAATGGA TGATAATGGA AATTCCGAGT 1101 ATTCTGGATT TGTAAATCCT GTATTAGAAC TGTCTGATTC TGGCATAAGG 1151 CATTCTGACA CAGATCAACA GACTCGATAG GGTAAAATTG TGTGACCTTG 1201 TTTATCAGTT ATGACCAAAT GTTAAAAACC AACTAGAATG TATAAGTGAT 1251 TGTGCTTAGC CTTTTTGTAA GGGAGATGTG TAAGAAACCA TGCTGTAAAT 1301 GCTTATTTTA TTACAAAGGA GTAGGGATGA TAGGATCTGA ATTGATACAG 1351 AATTAAGTGC AATTTCATCA TCTGCCTTCT GCTTTTCAAG ACCAATTTAA 1401 TGGTCCTGTC ATGTTACTGA TTAAATTTAC TTTGTCTTGT CTTTATAGCA 1451 TTTCTGTTTA CTATGGTAGA TTTCCACTTT CAATTTTTAA AATTAATTTT 1501 ACTTTGAATG ATTTATGAAG CCTATTTCAT TGTCTAACTA TGAAAATATT 1551 AAGACTTTTT TGTTAATTCT CAGCCGATGT GAAGGAAGCA TGAGGAGGGA 1601 TCGTCAGACT CAGATTTAGA ATAGTGTTCC CGTTTCCAGC ATTATTTATT 1651 TCTATGACTT CTTTGGATTT TATTATCTAA TAGTAAGTAC AGTTGATGTG 1701 GGTAGATGAC TCTAAGAAAT GCTGAAGTAT CGGCATTACA TGTGTTTATT 1751 TACATGTCCT AGTTTGATAA TGTTGATTCA ATCTGAACAA AAGATAATAT 1801 AAAAATAACC CTTCAGAGTT TGGACATTTC AAGTTGGTAA TAATAAAAAA 1851 TAATATTTAA GAAGATATAT ATATATATAT ATTTAGTTTT TTCCACTTCA 1901 TTTTACATGC CACTATATTG ACTTTAATTG ATATACAGTA TTAAGTTTTT 1951 AGGTGCCATT ATTTTTAAAA AATTCTATAT TTCCAATGAA CGATGTTAGA 2001 TTTTACACAG AACATATTCT CTGCATGATT TCAGAAAAGA AAATCTAAAA 2051 AGGTAATACG GGTATTTCAA ATAAAATCCT TTCTGGTATG AAAGGCTCCA 2101 TTGATTTTAT TAAGCCTTCC TTTACCTTGT AGTACAAGGT GCTTTAATGG 2151 GATAGAACTA AGCATATCAA TATCTATAAC TGCATTTTGT GCTAGACAAT 2201 TACTGTTCTT TTCTCTAAAA TGTATATGTC AATTTACAAG GCCAGGGATA 2251 GAAAACACTC CATAATTGCT TTCCTTGATT TTGCTGAGGA TTTGGTATGA 2301 TTTTAGTAAG CAAACTGTTT TTTGGTTTTT CCTTAATGTT TTTAATTTTT 2351 TTTCCTCTTG CAACAATGAC GGTGCATGTT CTTATAAATA TAGGAAGGTC 2401 CAGATATAAA TAGTAACCTA AAGTTCTTGC TGTGCTTAAA AAAAAAAATC 2451 ATGTGGCTCT TTCAATATTT GAACTGCTAA GCAATGACAT CTGTAGTTTT 2501 ATCTCCTTTT TTATGTCATA GAAATTAATA TGATACTTTA AATATGTAAA 2551 TATAATACAT TGGTAATGCT ATTATTTATA TCTGTCTTAA CATAATTTAA 2601 GTTGTAGCTG TGTCTTGGAA ATATTTTTAA GGTAATCTAT ATTCACATTG 2651 CCTGTGTTAA TGCTTTTTAA GGTTTGTATA CATCAGATGT ATATTTTTGG 2701 TTTGGCATAA GCTACGATTG TAATTTTTCT TGGCTTTTTG TTCATAAAGA 2751 ATTTTTTGAA GGAATGGTAA CAAATGGTAA TTTACAAATG GTTGTGAATA 2801 AACACATTTT TACACTTAAA AAAAAAAAAA AAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 272 bp to 1177 bp; peptide length: 302 Category: similarity to known protein

1 MGNCCWTQCF GLLRKEAGRL QRVGGGGGSK YFRTCSRGEH LTIEFENLVE

51 SDEGESPGSS HRPLTEEEIV DLRERHYDSI AEKQKDLDEK IQKELALQEE

101 KLRLEEEALY AAQREAARAA KQRKLLEQER QRIVQQYHPS NNGEYQSSGP

151 EDDFESCLRN MKSQYEVFRS SRLSSDATVL TPNTESSCDL MTKTKSTSGN

201 DDSTSLDLEW EDEEGMNRML PMRERSKTEE DILRAALKYS NKKTGSNPTS

251 ASDDΞNGLEW ENDFVSAEMD DNGNSEYSGF VNPVLELSDS GIRHSDTDQQ 301 TR

BLASTP hits

Entry A55817 from database PIR: cyclin-dependent kinase pl30-PITSLRE - mouse

Length = 783

Score = 123 (43.3 bits). Expect = 0.00013, P = 0.00013

Identities = 53/197 (26%), Positives = 96/197 (48%)

Alert BLASTP hits for DKFZphfbr2_2cl8, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_2cl8, frame 2

Report for DKFZphfbr2_2cl8.2

[LENGTH] 302

[MW] 34281.39

[pi] 4.73

[PROSITE] MYRISTYL 5

[PROSITE] CK2 PHOSPHO SITE 12

[PROSITE] TYR PHOSPHO SITE 2

[PROSITE] PKC PHOSPHO SITE 3

[KW] All Alpha

[KW] LOW COMPLEXITY 13.58 %

[KW] COILED COIL 13.58 %

SEQ MGNCCWTQCFGLLRKEAGRLQRVGGGGGSKYFRTCSRGEHLTIEFENLVESDEGESPGSS

SEG xxxxx

PRD ccccccccchhhhhhhhhheeecccccccceeeeccccccchhhhhhhhccccccccccc

COILS

SEQ HRPLTEEEIVDLRERHYDSIAEKQKDLDEKIQKELALQEEKLRLEEEALYAAQREAARAA

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD ccchhhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ KQRKLLEQERQRIVQQYHPSNNGEYQSSGPEDDFESCLRNMKSQYEVFRSSRLSSDATVL

SEG xxxxxxx

PRD hhhhhhhhhhhhhhhhccccccccccccccccchhhhhhhhhhhheeeeecccccceeee

COILS CCCCCCCCC SEQ TPNTESSCDLMTKTKSTSGNDDSTSLDLEWEDEEGMNRMLPMRERSKTEEDILRAALKYS

SEG

PRD ccccccccccccccccccccccccchhhhhhhccccccchhhhhhhcchhhhhhhhhhhc

COILS

SEQ NKKTGSNPTSASDDSNGLEWENDFVSAEMDDNGNSEYSGFVNPVLELSDSGIRHSDTDQQ

SEG

PRD cccccccccccccccccccccccceeeecccccccccccccceeeecccccccccccccc

COILS

SEQ TR

SEG

PRD CC

COILS

Prosite for DKFZphfbr2_2cl8.2

PS00005 60->63 PKC PHOSPHO SITE PDOC00005

PS00005 170->173 PKC PHOSPHO ^"SITE PDOC00005

PS00005 240->243 PKC PHOSPHO^{" "}SITE PDOC00005

PS00006 36->40 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 65->69 CK2 PHOSPHO SITE PDOC00006

PS00006 79->83 CK2 PHOSPHO SITE PDOC00006

PS00006 148->152 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 163->167 CK2 PHOSPHO- ^"SITE PDOC00006

PS00006 186->190 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 198->202 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 204->208 CK2 PHOSPHO- ^"SITE PDOC00006

PS00006 226->230 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 228->232 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 250->254 CK2 PHOSPHO ^'SITE PDOC00006

PS00006 295->299 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00007 103->111 TYR PHOSPHO ^"SITE PDOC00007

PS00007 103->111 TYR PHOSPHO ^"SITE PDOC00007

PS00008 24->30 MYRISTYL PDOC00008

PS00008 25->31 MYRISTYL PDOC00008

PS00008 199->205 MYRISTYL PDOC00008

PS00008 245->251 MYRISTYL PDOC00008

PS00008 291->297 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_2cl8.2)

DKFZphfbr2_2dl5

group: di erentiation/development

DKFZphfbr2_2dl5 encodes a novel 438 amino acid protein similarity to Mus musculus testis- specific Y-encoded-like protein (Tspyll) .

The TSPY genes are arranged in clusters on the Y chromosome of many mammalian species. TSPY is believed to function in early spermatogenesis and is a candidate for GBY, the putative gonadoblastoma-inducing gene on the Y. The novel protein is a new member of the TSPY-SET- NAP1L1 family, which represents proteins closely related to TSPY. Therefore, the new protein seems to be involved in early spermatogenesis.

The new protein can find application in modulating early spermatogenesis. strong similarity to testis-specific Y-encoded-like protein complete cDNA, complete eds, EST hits localisation: primer B does not match perfect

Sequenced by Qiagen

Locus: /map="729.2 cR from top of Chr6 linkage group"

Insert length: 3229 bp

Poly A stretch at pos. 3206, polyadenylation signal at pos. 3184

1 GGAGACTGTA GGGTGGGCGG TGCGAGCGGC GGTTAGCTCC CAGTTCGGCC

51 TCTGAGGAAA ACGGGCGTTC GCCTGCGGTT GGTCCGACTG TTAGCAACAT

101 GAGCGGCCTG GATGGGGTCA AGAGGACCAC TCCCCTCCAA ACCCACAGCA

151 TCATTATTTC TGACCAAGTC CCGAGCGACC AGGACGCACA CCAGTACCTG

201 AGGCTCCGCG ACCAAAGCGA GGCGACACAG GTGATGGCGG AGCCGGGTGA

251 GGGAGGCTCG GAGACCGTCG CGCTCCCGCC TTCACCGCCT TCAGAGGAGG

301 GGGGCGTACC CCAGGATCCC GCGGGCCGTG GCGGTACTCC CCAGATCCGA

351 GTTGTTGGGG GTCGCGGTCA TGTGGCGATC AAAGCCGGGC AGGAAGAGGG

401 CCAGCCTCCC GCCGAAGGCC TGGCAGCCGC TTCTGTGGTG ATGGCAGCCG

451 ACCGCAGCCT GAAAAAGGGC GTTCAGGGTG GAGAGAAGGC CCTAGAAATC

501 TGTGGCGCCC AGAGATCCGC GTCTGAGCTG ACGGCGGGGG CGGAGGCTGA

551 GGCGGAGGAG GTGAAGACAG GAAAGTGCGC CACCGTCTCA GCAGCCGTGG

601 CTGAGAGGGA GAGCGCTGAG GTGGTGGTGA AGGAAGGCCT GGCGGAGAAG

651 GAGGTAATGG AGGAGCAGAT GGAGGTAGAG GAGCAGCCGC CAGAAGGTGA

701 AGAAATAGAA GTGGCGGAGG AGGATAGATT GGAGGAGGAG GCGAGGGAGG

751 AAGAAGGGCC CTGGCCTTTG CATGAGGCTC TCCGCATGGA CCCTCTGGAG

801 GCCATCCAGC TGGAACTGGA CACTGTGAAT GCTCAGGCCG ACAGGGCCTT

851 CCAACAGCTG GAGCACAAGT TTGGGCGGAT GCGTCGACAC TACCTGGAGC

901 GGAGGAACTA CATCATTCAG AATATCCCGG GCTTCTGGAT GACTGCTTTT

951 CGAAACCACC CCCAGTTGTC CGCCATGATT AGGGGCCAAG ATGCAGAGAT

1001 GTTAAGGTAC ATAACCAATT TAGAGGTGAA GGAACTCAGA CACCCTAGAA

1051 CCGGTTGCAA GTTCAAGTTC TTCTTTAGAA GAAACCCCTA CTTCAGAAAC

1101 AAGCTGATTG TCAAGGAATA TGAGGTAAGA TCCTCCGGCC GAGTGGTGTC

1151 TCTTTCTACT CCAATTATAT GGCGCAGGGG GCATGAACCC CAGTCCTTCA

1201 TTCGCAGAAA CCAAGACCTC ATCTGCAGCT TCTTCACTTG GTTTTCAGAC

1251 CACAGCCTTC CAGAGTCCGA CAAAATTGCT GAGATTATTA AAGAGGATCT

1301 GTGGCCAAAT CCACTGCAAT ACTACCTGTT GCGTGAAGGA GTCCGTAGAG

1351 CCCGACGTCG CCCGCTAAGG GAGCCTGTAG AGATCCCCAG GCCCTTTGGG

1401 TTCCAGTCTG GTTAACATTT GCCCTTGGGA ATACTCCTGC ACAAGGTCTC

1451 CTACCACCTT CTGCTGGACC TGTGCTTGGG CATCAGCAAT GAGTATGCCT

1501 TCTATTGTGC TTTGTTTTTG CTGACTTTTC TGCACCCTGT TTCCTTTGGA

1551 TATTCAGTTC TCTCAACCTC AAGATTGAGA CGGTGGTGGG TATGCTTCTC

1601 CACTTCCATA TGACCTTCAT GCTGTTCTGG AATATCACAT GCTACGAGGT

1651 CATCCTTCAC ACTACTTGTA AGCCAAGCAA ATGATACTGT AGATTGTACT

1701 GCCTTTATCT GCACTGCTTG GACCCTGTTT ATTCCCAGGG CCTCTGAACT

1751 GGTTGCTGTC ACTTGGATTT CTAGCTTTGG GAGCCTGTTC CACCTACTCA

1801 GCTCTGCATT GAGCAGTATG GGCACATGCC CTGTGGACAG TTACTGGACG

1851 TTAATGAACT CAGAGGAGAA AAGCAGTGAG CCACTTGTTC TGTGTGATTT

1901 ATGGTACTTC ATTGCTCTTC CTTCACCTCT AGTCACTTTC TATTGCTACC

1951 TGCCCTACAT TGGCTCCTGC CAAGGTCCCT CTCTCTCCCT GTTTTCCTTT

2001 TTTTGAGACG GAGGACGGAG TCTTGCTCTG

2051 TCGCCCAGGT TGGAGTGCAG TGGCGCGATC TCGGCTCACT GCAACCTCCA

2101 CCTCCCGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTAGCTGGGA

2151 CTACAGGCGC GCGCCGCCAC GCCCGGCTAA TTTTTATATT TTTAGTAGAG

2201 ACGGGGTTTC ACCATGCTGG CCAGGCTGGT CTCGAACCCC GACCTCGTGA

2251 TCCGCCCTCC TTAGCCTCCC AATCCTCTCT TAAAAAAGTG ATAGCTCAGA

2301 AATATTTGTA AAAGCAAGGT TTTTATTTCA TTTTGGCTCT GTCATTTTCA

2351 GAGGCAAAGA AGTTGGCCTG TAAAATAGAG TGCTAGAGCT CTTACGCCCC

2401 TCCCCTTCTT CCCAACTTCC TACTTCCTAG CCCTTTTATC AACTCCTAGA

2451 ATAGTTAAAG AGAGACACAT CTAGATGGGA TGAAAGGTGC CCTAAGCAGG 2501 AGAAACTGAA CAAAAGGCTA GAGGCATGGG CCAGGTAAAA ATTGGGCCTA

2551 GAGTGAAGAC TGTGCTGCCG TTAAGAGCTT TCGAGGAAGG AGTACTTACT

2601 CCCCAATGAT GATGAATGGA GAAATACTTT TCAGGGAGAA TTGAAGGGGT

2651 TAAAGTGTTA AATATGTTGC CTAGACAAGG GTTCTTTAAA GAAAGACAGC

2701 GCAACTTTGA ATGCTTTCTT ACTTGTTTTG TGACCTAATT TATGTGGAAG

2751 ATTGTTATTT CATTAGGATT TAGTAAAATT TTTTTTTCTG ATTCTAAACT

2801 TATTGTGAAA ATTGAGCTGT ACAGATATTC TTTTGATTTC AATTGGGAAC

2851 ATTTGGAAGA ACAACAGTCT TACTTGCCTG TACAATATAG AGACATATGA

2901 ATAGTCATAA CAGTTTTCAA CTTGTTCTTG TTTCTGTTAA ACTATATTCC

2951 TAGAAACATA GTTTGAACAA CTTGGTCTTT GTTAGGCTTG TCAAATTGCC

3001 TTCATGGAAA AATAATCTAC AAAAGTATGG TTTAATTGAT TGTCTTACAT

3051 GATAATTTTC CCTGGCAACA ACTTAGTAAG TGATATATCT TTTTTCCTAA

3101 ATTGCTTAAA TACTGTGAAA TTGCTCTGAC AAATTGGAAG TGTACCATTG

3151 GCATATTTGT CTTCCTTTTT ATGCATGATG GTAAAATAAA AGCATGTTGT

3201 TCTGCTAAGA AAAAAAAAAA AAAAAAAAA

BLAST Results

Entry AF042181 from database EMBLNEW:

Homo sapiens testis-specific Y-encoded-like protein (TSPYL) mRNA, partial eds. Score = 3411, P = 6.9e-148, identities = 685/687

Entry HS938343 from database EMBL: human STS WI-11947. Score = 1195, P = 2.1e-46, identities = 273/299

Medline entries

98399864:

Murine and human TSPYL genes: novel members of the TSPY-SET-NAPILI family

Peptide information for frame 3

ORF from 99 bp to 1412 bp; peptide length: 438 Category: strong similarity to known protein Classification: Differentiation/Development

1 MSGLDGVKRT TPLQTHSIII SDQVPSDQDA HQYLRLRDQS EATQVMAEPG 51 EGGSETVALP PSPPSEEGGV PQDPAGRGGT PQIRVVGGRG HVAIKAGQEE 101 GQPPAEGLAA ASVVMAADRS LKKGVQGGEK ALEICGAQRS ASELTAGAEA 151 EAEEVKTGKC ATVSAAVAER ESAEVVVKEG LAEKEVMEEQ MEVEEQPPEG 201 EEIEVAEEDR LEEEAREEEG PWPLHEALRM DPLEAIQLEL DTVNAQADRA 251 FQQLEHKFGR MRRHYLERRN YIIQNIPGFW MTAFRNHPQL SAMIRGQDAE 301 MLRYITNLEV KELRHPRTGC KFKFFFRRNP YFRNKLIVKE YEVRSSGRVV 351 SLSTPIIWRR GHEPQSFIRR NQDLICSFFT WFSDHSLPES DKIAEIIKED 401 LWPNPLQYYL LREGVRRARR RPLREPVEIP RPFGFQSG

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_2dl5, frame 3

TREMBL:AF042180_1 gene: "Tspyll"; product: "testis-specific Y-encoded-like protein"; Mus musculus testis-specific Y-encoded-like protein (Tspyll) mRNA, complete eds., N = 1, Score = 1202, P = 3.1e-122

TREMBL:AB018264_1 gene: "KIAA0721"; product: "KIAA0721 protein"; Homo sapiens mRNA for KIAA0721 protein, partial eds., N = 1, Score = 798, P = 2e-79

TREMBL :AB015345_1 gene: "HRIHFB2216"; Homo sapiens HRIHFB2216 mRNA, partial eds., N = 1, Score = 570, P = 2.9e-55

>TREMBL:AF042180_1 gene: "Tspyll"; product: "testis-speci ic Y-encoded-like protein"; Mus musculus testis-specific Y-encoded-like protein (Tspyll) mRNA, complete eds . Length = 379

HSPs: Score = 1202 (180.3 bits), Expect = 3.1e-122, P = 3.1e-122 Identities = 258/377 (68%), Positives = 283/377 (75%)

Query: 62 SPPSEEGGVPQDPAGR GGTPQIRVVGGRGHVAIKAGQEE—GQP-P—AEGLAA 110

SP +EG D G GTP R + G G+ G P P EGL Sbjct: 3 SPERDEGTPVPDSRGHCDADTVSGTPDRRPLLGEEKAVTGEGRAGIVGSPAPRDVEGLVP 62

Query: 111 ASVVMAADRSLKK-GVQGGEKALEICGAQRSASELTAGAEAEAEEVKTGKCATVΞAAVAE 169

V AA + V+G A+ + ++ T GAE++A +VKT + TV+AA Sbjct: 63 QIRVAAARQGESPPSVRGPAAAVFVTPKYVEKAQETRGAESQARDVKT-EPGTVAAAA— 119

Query: 170 RESAEVVVKEGLAEKEVMEEQMEVEEQPPEGEEIEVAEEDRLEEEAREEEGPWPLHEALR 229

E +EV EE MEVE Q P GEE+E+ E EA EE GPW L LR

Sbjct: 120 -EKSEVATPGS EEVMEVE-QKPAGEEMEMLEAΞGGVREAPEEAGPWHLGIDLR 170

Query: 230 MDPLEAIQLELDTVNAQADRAFQQLEHKFGRMRRHYLERRNYIIQNIPGFWMTAFRNHPQ 289

+PLEAIQLELDTVNAQADRAFQ LE KFGRMRRHYLERRNYIIQNIPGFWMTAFRNHPQ Sbjct: 171 RNPLEAIQLELDTVNAQADRAFQHLEQKFGRMRRHYLERRNYIIQNIPGFWMTAFRNHPQ 230

Query: 290 LSAMIRGQDAEMLRYITNLEVKELRHPRTGCKFKFFFRRNPYFRNKLIVKEYEVRSSGRV 349

LSAMIRG+DAEMLRY+T+LEVKELRHP+TGCKFKFFFRRNPYFRNKLIVKEYEVRSSGRV Sbjct: 231 LSAMIRGRDAEMLRYVTSLEVKELRHPKTGCKFKFFFRRNPYFRNKLIVKEYEVRSSGRV 290

Query: 350 VSLSTPIIWRRGHEPQSFIRRNQDLICSFFTWFSDHSLPESDKIAEIIKEDLWPNPLQYY 409

VSLSTPIIWRRGHEPQSFIRRNQDLICSFFTWFΞDHSLPESD+IAEIIKEDLWPNPLQYY Sbjct: 291 VSLSTPIIWRRGHEPQSFIRRNQDLICSFFTWFSDHSLPESDRIAEIIKEDLWPNPLQYY 350

Query: 410 LLREGVRRARRRPLREPVEIPRPFGFQSG 438

L REG+RR RRRP+REPVEIPRPFGFQSG Sbjct: 351 LCREGIRRPRRRPIREPVEIPRPFGFQSG 379

Pedant information for DKFZphfbr2_2dl5, frame 3

Report for DKFZphfbr2_2dl5.3

[LENGTH] 438

[MW] 49307.65

[pi] 5.36

[HOMOL] TREMBL:AF042180_1 gene: "Tspyll"; product: "testis-specific Y-encoded-like protein"; Mus musculus testis-specific Y-encoded-like protein (Tspyll) mRNA, complete eds. le-

107

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YKR048c] le-07

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YKR048c] le-07

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YKR048c] le-07

[FUNCAT] 09.13 biogenesis of chromosome structure [S. cerevisiae, YKR048c] le-07

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKR048c] le-07

[BLOCKS] BL00376F

[PIRKW] nucleus 6e-39

[PIRKW] DNA binding 3e-06

[PIRKW] phosphoprotein 6e-39

[PIRKW] alternative splicing 6e-39

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 22.83 %

SEQ MSGLDGVKRTTPLQTHSIIIΞDQVPSDQDAHQYLRLRDQSEATQVMAEPGEGGSETVALP

SEG x

PRD ccccccccccccccceeeeecccccccccchhhhhhhhchhhhhcccccccccceeeecc

SEQ PSPPSEEGGVPQDPAGRGGTPQIRVVGGRGHVAIKAGQEEGQPPAEGLAAASVVMAADRS

SEG xxxxxxxxx

PRD ccccccccccccccccccccceeeeecccceeeeecccccccccchhhhhhhhhhhhhcc

SEQ LKKGVQGGEKALEICGAQRSASELTAGAEAEAEEVKTGKCATVSAAVAERESAEVVVKEG

SEG xxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxx .

PRD ccccccccccceeeccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ LAEKEVMEEQMEVEEQPPEGEEIEVAEEDRLEEEAREEEGPWPLHEALRMDPLEAIQLEL SEG .xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhcccccccchhhhhhhhhhhhhhhh

SEQ DTVNAQADRAFQQLEHKFGRMRRHYLERRNYIIQNIPGFWMTAFRNHPQLSAMIRGQDAE SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccceeeeecccccccccccccchhh

SEQ MLRYITNLEVKELRHPRTGCKFKFFFRRNPYFRNKLIVKEYEVRSSGRVVSLSTPIIWRR SEG

PRD hhhhhhhhhhhhhcccccceeeeeeeccccccchhhhhhccccccccccccccceeeecc

SEQ GHEPQSFIRRNQDLICSFFTWFSDHSLPESDKIAEIIKEDLWPNPLQYYLLREGVRRARR

SEG xxxxxxxxxxx

PRD ccccchhhhhhcccccceeeeeccccccccchhhhhhhhhcccccceeeeccccchhhhh

SEQ RPLREPVEIPRPFGFQSG

SEG xxxxxxxx

PRD hccccccccccccccccc

(No Prosite data available for DKFZphfbr2_2dl5.3) (No Pfam data available for DKFZphfbr2_2dl5.3)

DKFZphfbr2_2dl7

group: transmembrane proteins

DKFZphfbr2_2dl7 encodes a novel 292 ammo acid protein with similarity to a C. elegans hypothetical protein.

One transmembrane region is predicted for the protein.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes and as a new marker for neuronal cells. similarity to C. elegans hypothetical protein

TRANSMEMBRANE 1

Sequenced by Qiagen

Locus : unknown

Insert length: 1009 bp

Poly A stretch at pos. 990, polyadenylation signal at pos. 969

1 TGGGCCTGTG GCTGGGGGCA GAGCTCAGAC TGTCTTCTGA AGATTGATGT 51 CTATTTCCTT GAGCTCTTTA ATTTTGTTGC CAATTTGGAT AAACATGGCA 101 CAAATCCAGC AGGGAGGTCC AGATGAAAAA GAAAAGACTA CCGCACTGAA 151 AGATTTATTA TCTAGGATAG ATTTGGATGA ACTAATGAAA AAAGATGAAC 201 CGCCTCTTGA TTTTCCTGAT ACCCTGGAAG GATTTGAATA TGCTTTTAAT 251 GAAAAGGGAC AGTTAAGACA CATAAAAACT GGGGAACCAT TTGTTTTTAA 301 CTACCGGGAA GATTTACACA GATGGAACCA GAAAAGATAC GAGGCTCTAG 351 GAGAGATCAT CACGAAGTAT GTATATGAGC TCCTGGAAAA GGATTGTAAT 401 TTGAAAAAAG TATCTATTCC AGTAGATGCC ACTGAGAGTG AACCAAAGAG 451 TTTTATCTTT ATGAGTGAGG ATGCTTTGAC AAATCCACAG AAACTGATGG 501 TTTTAATTCA TGGTAGTGGT GTTGTCAGGG CAGGGCAGTG GGCTAGAAGA 551 CTTATTATAA ATGAAGATCT GGACAGTGGC ACACAGATAC CGTTTATTAA 601 AAGAGCTGTG GCTGAAGGAT ATGGAGTAAT AGTACTAAAT CCCAATGAAA 651 ACTATATTGA AGTAGAAAAG CCGAAGATAC ACGTACAGTC ATCATCTGAT 701 AGTTCAGATG AACCAGCAGA AAAACGGGAA AGAAAAGATA AAGTTTCTAA 751 AGTAACAAAG AAGCGACGTG ATTTCTATGA GAAGTATCGT AACCCCCAAA 801 GAGAAAAAGA AATGATGCAA TTGTATATCA GAGTGAGTGA GATCACTACT 851 TTCCTTTACT ATTTTCTTTA CCTTGTATAT ATTTTATTAT ATGTAGATTG 901 TTTTGTTTTT CTTCAAGAAT ATTAATTTCT TTATTTGTCA TCATTTATTT 951 CCCATGGTCG TCTACTTGGA TTAAATGGGT TTTTAAATTC AAAAAAAAAA 1001 AAAAAAAAA

BLAST Results

Entry 189937 from database EMBL:

Sequence 11 from patent US 5723315.

Score = 1083, P = 2.2e-42, identities = 223/231

Entry 189938 from database EMBL:

Sequence 12 from patent US 5723315.

Score = 875, P = 7.4e-33, identities = 175/175

\

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 47 bp to 922 bp; peptide length: 292 Category: similarity to unknown protein Classification: unset

1 MSISLSSLIL LPIWINMAQI QQGGPDEKEK TTALKDLLΞR IDLDELMKKD 51 EPPLDFPDTL EGFEYAFNEK GQLRHIKTGE PFVFNYREDL HRWNQKRYEA

101 LGEIITKYVY ELLEKDCNLK KVSIPVDATE SEPKΞFIFMS EDALTNPQKL

151 MVLIHGSGVV RAGQWARRLI INEDLDSGTQ IPFIKRAVAE GYGVIVLNPN

201 ENYIEVEKPK IHVQSSSDSS DEPAEKRERK DKVSKVTKKR RDFYEKYRNP

251 QREKEMMQLY IRVSEITTFL YYFLYLVYIL LYVDCFVFLQ EY

BLASTP hits

Entry S67436 from database PIR: hypothetical protein - fission yeast (Schizosaccharomyces pombe)

Length = 266

Score = 112 (39.4 bits), Expect = 0.00037, P = 0.00037

Identities = 33/147 (22%), Positives = 69/147 (46%)

Entry CEY75B8A_12 from database TREMBLNEW: gene: "Y75B8A.31"; Caenorhabditis elegans cosmid Y75B8A

Score = 327, P = 1.5e-29, identities = 72/140, positives = 93/140

Alert BLASTP hits for DKFZphfbr2_2dl7, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_2dl7, frame 2

Report for DKFZphfbr2_2dl7.2

[LENGTH] 292

[MW] 34260.50

[pi] 5.50

[HOMOL] TREMBLNEW:AF064782_1 product: "unknown"; Mus musculus clone pEN87 unknown mRNA, partial eds. le-119

[KW] SIGNAL_PEPTIDE 19

[KW] TRANSMEMBRANE 1

[KW] LOW_COMPLEXITY 10.96 %

SEQ MSISLSSLILLPIWINMAQIQQGGPDEKEKTTALKDLLSRIDLDELMKKDEPPLDFPDTL

SEG . xxxxxxxxxxxxxx

PRD ccchhhhhhchhhhhhhccccccccccchhhhhhhhhhhhhcchhhhhhccccccccccc

MEM

SEQ EGFEYAFNEKGQLRHIKTGEPFVFNYREDLHRWNQKRYEALGEIITKYVYELLEKDCNLK

SEG

PRD hhhhhhcccccceeeecccccceeecccccccchhhhhhhhhhhhhhhhhhhhhhhhhhe

MEM

SEQ KVSIPVDATESEPKSFIFMSEDALTNPQKLMVLIHGSGVVRAGQWARRLIINEDLDSGTQ

SEG

PRD eeeccccccccccceeeeeeccccccccceeeeeecccccchhhhhcccccccccccccc

MEM

SEQ IPFIKRAVAEGYGVIVLNPNENYIEVEKPKIHVQSSSDSSDEPAEKRERKDKVSKVTKKR

SEG

PRD chhhhhhhhccceeeeeccccceeeeeccceeeeccccccccchhhhhhhhhhhhhhhhh

MEM

SEQ RDFYEKYRNPQREKEMMQLYIRVSEITTFLYYFLYLVYILLYVDCFVFLQEY

SEG xxxxxxxxxxxxxxxxxx

PRD hhhhhhhcccchhhhhhhhhhhhheeeeehhhhhhhhhhhhheeeeeeeccc

MEM MMMMMMMMMMMMMMMMMMMMM

(No Prosite data available for DKFZphfbr2_2dl7.2) (No Pfam data available for DKFZphfbr2_2dl7.2) DKFZphfbr2_2d20

group: brain derived

DKFZphfbr2_2d20 encodes a novel 197 amino acid protein with similarity to Synechocystis sp. P74594 hypothetιcal32.8 kD protein.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . similarity to Synechocystis sp. (PCC 6803) complete cDNA, complete eds, EST hits potential start at bp 67 matches kozak consensus ANCatgG

Sequenced by Qiagen

Locus: unknown

Insert length: 1787 bp

Poly A stretch at pos. 1768, polyadenylation signal at pos. 1743

1 TGGGGCGGCC GCGGCGGGAA CATGGAGGAG CTGCTGAGGC GCGAGCTGGG

51 CTGCAGCTCT GTCAGGGCCA CGGGCCACTC GGGGGGCGGG TGCATCAGCC

101 AGGGCCGGAG CTACGACACG GATCAAGGAC GAGTGTTCGT GAAAGTGAAC

151 CCCAAGGCGG AGGCCAGAAG AATGTTTGAA GGTGAGATGG CAAGTTTAAC

201 TGCCATCCTG AAAACAAACA CGGTGAAAGT GCCCAAGCCC ATCAAGGTTC

251 TGGATGCCCC AGGCGGCGGG AGCGTGCTGG TGATGGAGCA CATGGACATG

301 AGGCATCTGA GCAGTCATGC TGCAAAGCTT GGAGCCCAGC TGGCCGATTT

351 ACACCTTGAT AACAAGAAGC TTGGAGAGAT GCGCCTGAAG GAGGCGGGCA

401 CAGTGTGGAG AGGAGGTGGG CAGGAGGAAC GGCCCTTTGT GGCCCGGTTT

451 GGATTTGACG TGGTGACGTG CTGTGGATAC CTCCCCCAGG TGAATGACTG

501 GCAGGAGGAC TGGGTCGTGT TCTATGCCCG GCAGCGCATT CAGCCCCAGA

551 TGGACATGGT GGAGAAGGAG TCTGGGGACA GGGAGGCCCT CCAGCTTTGG

601 TCTGCTCTGC AGTAAAAGAT CCCTGACCTG TTCCGTGACC TGGAGATCAT

651 CCCAGCCTTA CTCCACGGGG ACCTCTGGGG TGGAAACGTA GCAGAGGATT

701 CCTCTGGGCC GGTGATTTTT GACCCAGCTT CTTTCTACGG CCACTCGGAA

751 TATGAGCTGG CAATAGCTGG CATGTTTGGG GGCTTTAGCA GCTCCTTTTA

801 CTCCGCCTAC CACGGCAAAA TCCCCAAGGC CCCAGGATTC GAGAAGCGCC

851 TTCAGTTGTA TCAGCTCTTT CACTACTTGA ACCACTGGAA TCATTTTGGA

901 TCGGGGTACA GAGGATCCTC CCTGAACATC ATGAGGAATC TGGTCAAGTG

951 AGCGGGCCTT ACTCTGGAAG GAGGTCTCAG AGGTTTCTCC ACAGTCCTCT

1001 TCTGGGCAAA TTCTTGTTTC TTCACATGCC GGACTAGCTT AAGACCAATG

1051 CAGTAGCTTA TTTCCAAGCC TTGCAAAGTA TATAATATCT AAGAGGAAAG

1101 GTTTTGTCAT CCCAGCGTTG TCCACTTTGT GGGGCTTTGT AGGTAGACGG

1151 AGCCACACTA CAGGCAGGGT ATGAGCAGAG GGATGTATGG AGTGTGGGCG

1201 ACTCTGAGCC TCACTGCTGC TGCAAGGTGG GGAAACTGTA AGTGAACCCC

1251 TGTGGGTGCG GGGGAGGGTA TCCGGTGCGC AGGGAGGTGG CCAGCGCCCC

1301 CGGGCACTGC TGCTCATAGG TACCTTTCCG CTGCCTCCTC CCTGCTCTCC

1351 TGTGCAGGAA TGTCTCTGAG CTGTTCACGT TGATGCTTCT TGGTTGGCAA

1401 GACTTGGGTG TAGACATGAA ACCACCTTAC TAAAAGCGTC TTAAAATGAC

1451 CAATTCCAGA ATCAAGCGTA TTCCGTTTTC CTCCTGCATG ATCCCTGGGC

1501 CCTCCCGCAG GCTGAGCAAG TCTGTAAACT GATTCTGGGA GAAACCAAGC

1551 TGCTGGCCGT AGGATGTCCT TGGGTACATC CAGGAGTCTT CATTGCTTCT

1601 GTTATTACCC CGTCTCCTCT GCCATTTTCT ACAGCTTGCT GAGTTGTCAT

1651 TCCTTTGCAA CATTAAAATA CATGCTGAAC TCATATTTTT CCTTCCTTCA

1701 CTGTTGTAGT AAAGAGACAT ATTTCATGAA TGGCATTGAT GCTAATAAAC

1751 CCTTTGCCCA AAAATTTGAA AAAAAAAAAA AAAAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 1 ORF from 22 bp to 612 bp; peptide length: 197 Category: similarity to unknown protein Prosite motifs: LEUCINE ZIPPER (117-139)

1 MEELLRRELG CSSVRATGHS GGGCISQGRS YDTDQGRVFV KVNPKAEARR 51 MFEGEMASLT AILKTNTVKV PKPIKVLDAP GGGSVLVMEH MDMRHLSSHA 101 AKLGAQLADL HLDNKKLGEM RLKEAGTVWR GGGQEERPFV ARFGFDVVTC 151 CGYLPQVNDW QEDWVVFYAR QRIQPQMDMV EKESGDREAL QLWSALQ

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_2d20, frame 1

No Alert BLASTP hits found

Pedant information for DKFZphfbr2_2d20, frame 1

Report for DKFZphfbr2_2d20.1

[LENGTH] 197

[MW] 21963.25

[pi] 6.96

[HOMOL] PIR:S76790 hypothetical protein - Synechocystis sp. (strain PCC 6803) 9e-12

[SUPFAM] hypothetical protein bl725 le-06

[PROSITE] LEUCINE_ZIPPER 1

[PROSITE] MYRISTYL 2

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE 2

[KW] Alpha_Beta

SEQ MEELLRRELGCSSVRATGHSGGGCISQGRSYDTDQGRVFVKVNPKAEARRMFEGEMASLT PRD ccchhhhhccccceeeeccccccceeeccccccccceeeeeeccchhhhhhhhhhhhhhh

SEQ AILKTNTVKVPKPIKVLDAPGGGSVLVMEHMDMRHLΞSHAAKLGAQLADLHLDNKKLGEM PRD hhhhhheeeeccceeeecccccceeeeecccccccchhhhhhhhhhhhhhhcccccchhh

SEQ RLKEAGTVWRGGGQEERPFVARFGFDVVTCCGYLPQVNDWQEDWVVFYARQRIQPQMDMV PRD hhhhhccccccccccccceeeccccceeeccccccccccccchhhhhhhhhhhhhhhhhh

SEQ EKEΞGDREALQLWSALQ PRD hhhccchhhhhhhhccc

Prosite for DKFZphfbr2_2d20.1

PS00002 20->24 GLYCOSAMINOGLYCAN PDOC00002 PS00005 13->16 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 67->70 PKC_PHOSPHO_SITE PDOC00005 PS00008 22->28 MYRISTYL PDOC00008 PS00008 104->110 MYRISTYL PDOC00008 PS00029 96->118 LEUCINE ZIPPER PDOC00029

(No Pfam data available for DKFZphfbr2_2d20.1) DKFZphfbr2_2gl8

group: brain derived

DKFZphfbr2_2gl8 encodes a novel 229 amino acid protein with partial similarity to the humane dJ30M3.2 gene product.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of bram-specific genes .

J30M3.2 extension of genmodel complete cDNA, complete eds, EST hits (mouse ESTs with >90% Identities)

Sequenced by Qiagen

Locus: /map="6p22.1-22"

Insert length: 2444 bp

Poly A stretch at pos. 24 5, no polyadenylation signal found

1 TGGTCGAGGG TCGACGGTAT CGATAAGTTT TTTTTTTTTT TTTTTTTTTT

51 TGGAAAGCAA GGATCACACT TCCCCCTCCC TGTTCCTTAA TCCCTTTTCT

101 AAAAAGGGGG GAAAATCCGG ATGGATTTTA GGGATTGGTC TGGTGTCAGC

151 TGTGTCTTAT TGCACACCTA AATCCTGATT ATAGGCTTTT CATTTCTCCG

201 CAAAGCCTTT ATTTTGGCAG TTAAGCCAAA TGTGTTTTCC AGAAAGTTAG

251 TTATTTTCTC CTCTTTCTTT CCTTTCTTTC CTCCCTTTTT CCCGTCTGAC

301 CCCAAACGTT ATTGTCCAAA CATGACTGGA CAGCAGCTTT TGTTTCTTGA

351 CCCTGTAATA TGACAGTCTG CTAATATTGA CAGAAGGTGC AGTTTTTGGG

401 TTATAGTCGT GATTTTCGCT AATCAATCAT ATTAGCAGGA AAAAAAATGA

451 CTTGTTTCTG TTGTACTTGA GTCTTAAGAA AAAGTGCCCA TAGTTTAGTG

501 ACAATTTCCA AAGGCTTTAG TACCACCTGT ATTTCAAAAT GGGGGACCCA

551 AACTCCCGGA AGAAACAAGC TCTGAACAGA CTACGTGCTC AGCTTAGAAA

601 GAAAAAAGAA TCTCTAGCTG ACCAGTTTGA CTTCAAGATG TATATTGCCT

651 TTGTATTCAA GGAGAAGAAG AAAAAGTCAG CACTTTTTGA AGTGTCTGAG

701 GTTATACCAG TCATGACAAA TAATTATGAA GAAAATATCC TGAAAGGTGT

751 GCGAGATTCC AGCTATTCCT TGGAAAGTTC CCTAGAGCTT TTACAGAAGG

801 ATGTGGTACA GCTCCATGCT CCTCGATATC AGTCTATGAG AAGGGATGTA

851 ATTGGCTGTA CTCAGGAGAT GGATTTCATT CTTTGGCCTC GGAATGATAT

901 TGAAAAAATC GTCTGTCTCC TGTTTTCTAG GTGGAAAGAA TCTGATGAGC

951 CTTTTAGGCC TGTTCAGGCC AAATTTGAGT TTCATCATGG TGACTATGAA

1001 AAACAGTTTC TGCATGTACT GAGCCGCAAG GACAAGACTG GAATCGTTGT

1051 CAACAATCCT AACCAGTCAG TGTTTCTCTT CATTGACAGA CAGCACTTGC

1101 AGACTCCAAA AAACAAAGCT ACAATCTTCA AGTTATGCAG CATCTGCCTC

1151 TACCTGCCAC AGGAACAGCT CACCCACTGG GCAGTTGGCA CCATAGAGGA

1201 TCACCTCCGT CCTTATATGC CAGAGTAGAG TACTGACCAG CAAAATGGAG

1251 AAGATCAGAG AATGCAGCAG CAGTTTTTTT TCTTGTTTTC TTACCACTTT

1301 ATTCTTTCAG AGTTTAAAGA AAATGGACTC ATGCACAGAA CACTATGCAT

1351 TTTGAAACTT GTTCATCCTG GATTTTTTTA AATCATTTTT ATCTCAGAAC

1401 TTAAACAAAA ATTAGATGTC GTGCACGGAC TGTGTGAAAG AAGATGCTTT

1451 GCATATTTGC TGCACTGCAT CAGTATCTTA CTAAAAATGT GAAATGAAAG

1501 GACTATTGTA CACTGAAATG CTTAAATGTA TCTGAAAGCA CAAGGTGATA

1551 CTCATTTTTA TGGTCTTCCC ATTTGTGCTG GTTTTTGCCT CTTTGACATC

1601 TGTCATCAGT ATTTAGAGGG TGAGAAGTGA ATGTAACAGG TATAAATAAC

1651 ATTTTTAAAA ACAATAACTT TGCTATAATC ACAGTTGTTC CAGAGCACTG

1701 TCAGATACAT TCTAATGACC AGAACTGGTT TAAAAAAAGA AAATACAACC

1751 ATGGGAAAGA AATCTTAAAT GAAAAACGCA TCTCATTGTA GGCATTTTTG

1801 CCTCATATTT TACTGGGCCA TGTTTGTTTC CTGGTACTCA TGTATTTTTT

1851 TTTTTTCCAG ATCTCTTTCC CCAAGTTGCT ATTGTAAGAG TATTCTGCTG

1901 CGTGTGGATG CAGTTATACA CATTAAAGCA GATCTGGAGT CTGAAGTAGC

1951 TATAAAGCAG CTATAAAACA GAAATACATG CATAGCTGCA GAAACCATGA

2001 TAGGTAGAGG ACTTTTCTTT TGGTTTTGTT TTGTTTTGTT TTGTTTTGTT

2051 TTTGGTTTTA CAGAGAAGAG ATTTTTATTA CAAAGAAAAA AATTCCAGTG

2101 AATTGTGCAG AAATGCTGGT TTTTACACCA TCCTAAAGAA AAACTTTACA

2151 AGGGTGTTTT GGAGTAGAAA AAAGGTTATA AAGTTGGAAT CTTAAATTGT

2201 AAAATTAACC ATTGAGTGTC AAAGTTCTAA AAGCAGAACT CATTTCGTGC

2251 AATGAACATA AGGAAAGACT ACTGTATAGG TTTTTTTTTT TCTCCTTTTA

2301 AATGAAGAAA AGCTTTGCTT AAGGGTTGCA TACTTTTATT GGAGTAAATC

2351 TGAATGATCC TACTCCTTTG GAGTAAGACT AGTGCTTACC AGTTTCCAAT

2401 TGTATTTAGC TTCTGTTGGA ATTTGAAAAA AAAAAAAAAA AAAA

BLAST Results Entry HS338352 from database EMBL: human STS EST171398. Score = 1747, P = 3.0e-74, identities = 359/365

Entry HS447255 from database EMBL: human STS SHGC-10143. Score = 1717, P = 6.5e-73, identities = 365/383

Entry HS30M3 from database EMBLNEW:

Human DNA sequence from clone 30M3 on chromosome 6p22.1-22.3. Contains three novel genes, one similar to C. elegans Y63D3A.4 and one similar to (predicted) plant, worm, yeast and archaea bacterial genes, and the first exon of the KIAA0319 gene. Contains ESTs, GSSs and putative CpG islands . Score = 6646, P = O.Oe+00, identities = 1344/1355

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 539 bp to 1225 bp; peptide length: 229 Category: putative protein

1 MGDPNSRKKQ ALNRLRAQLR KKKESLADQF DFKMYIAFVF KEKKKKSALF

51 EVSEVIPVMT NNYEENILKG VRDSSYSLES SLELLQKDVV QLHAPRYQSM

101 RRDVIGCTQE MDFILWPRND IEKIVCLLFS RWKESDEPFR PVQAKFEFHH

151 GDYEKQFLHV LSRKDKTGIV VNNPNQSVFL FIDRQHLQTP KNKATIFKLC

201 SICLYLPQEQ LTHWAVGTIE DHLRPYMPE

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_2gl8, frame 2

TREMBLNEW:HS30M3_2 gene: "dJ30M3.2"; product: "dJ30M3.2 (novel protein)"; Human DNA sequence from clone 30M3 on chromosome 6p22.1-22.3. Contains three novel genes, one similar to C. elegans Y63D3A.4 and one similar to (predicted) plant, worm, yeast and archaea bacterial genes, and the first exon of the KIAA0319 gene. Contains ESTs, GSSs and putative CpG islands., N = 1, Score = 470, P = l.le-44

>TREMBLNEW:HS30M3_2 gene: "dJ30M3.2"; product: "dJ30M3.2 (novel protein)"; Human DNA sequence from clone 30M3 on chromosome 6p22.1-22.3. Contains three novel genes, one similar to C. elegans Y63D3A.4 and one similar to (predicted) plant, worm, yeast and archaea bacterial genes, and the first exon of the KIAA0319 gene. Contains ESTs, GSSs and putative CpG islands. Length = 86

HSPs:

Score = 470 (70.5 bits), Expect = l.le-44, P = l.le-44 Identities = 86/86 (100%), Positives = 86/86 (100%)

Query: 144 AKFEFHHGDYEKQFLHVLSRKDKTGIVVNNPNQSVFLFIDRQHLQTPKNKATIFKLCSIC 203

AKFEFHHGDYEKQFLHVLSRKDKTGIVVNNPNQSVFLFIDRQHLQTPKNKATIFKLCSIC Sbjct: 1 AKFEFHHGDYEKQFLHVLSRKDKTGIVVNNPNQSVFLFIDRQHLQTPKNKATIFKLCSIC 60

Query: 204 LYLPQEQLTHWAVGTIEDHLRPYMPE 229

LYLPQEQLTHWAVGTIEDHLRPYMPE Sbjct: 61 LYLPQEQLTHWAVGTIEDHLRPYMPE 86

Pedant information for DKFZphfbr2_2gl8, frame 2 Report for DKFZphfbr2_2gl8.2 [LENGTH] 229

[MW] 27083.42

[pi] 9.04

[HOMOL] TREMBL:HS30M3 2 gene "dJ30M3.2 " ; product : "dJ30M3 .2 (novel protein) ' Human

DNA sequence from clone 30M3 on chromosome 6p22.1-22.3. Contains three novel genes, one similar to C. elegans Y63D3A.4 and one similar to (predicted) plant, worm, yeast and archaea bacterial genes, and the first exon of the KIAA0319 gene. Contains ESTs, GSSs and putative CpG islands. 6e-47

[PROSITE] MYRISTYL 2

[PROSITE] CAMP_PHOSPHO_SITE 2

[PROSITE] CK2_PHOSPHO_SITE 4

[PROSITE) TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 4

[PROSITE] ASN_GLYCOSYLATION 1

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 5.24 %

SEQ MGDPNSRKKQALNRLRAQLRKKKESLADQFDFKMYIAFVFKEKKKKSALFEVSEVIPVMT SEG PRD cccccchhhhhhhhhhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhheeeeec

SEQ NNYEENILKGVRDSSYSLESSLELLQKDVVQLHAPRYQΞMRRDVIGCTQEMDFILWPRND SEG xxxxxxxxxxxx PRD cchhhhhhhcccccccccchhhhhhhhhhhhhhccccccccceeecccccceeeecccch

SEQ IEKIVCLLFSRWKESDEPFRPVQAKFEFHHGDYEKQFLHVLSRKDKTGIVVNNPNQSVFL SEG PRD hhhhhhhhhhhccccccccccccccccccccchhhhhhhhhhhcccceeeeccccceeee

SEQ FIDRQHLQTPKNKATIFKLCSICLYLPQEQLTHWAVGTIEDHLRPYMPE SEG PRD eeecccccccccceeeeeeeeeeeeeccccccccceeeecccccccccc

Prosite for DKFZphfbr2_2gl8.2

PS00001 175->179 ASN_GLYCOSYLATION PDOC00001

PS00004 22->26 CAMP_PHOΞPHO_SITE PDOC00004

PS00004 44->48 CAMP_PHOSPHO_SITE PDOC00004

PS00005 6->9 PKC_PHOSPHO_SITE PDOC00005

PS00005 99->102 PKC_PHOSPHO_SITE PDOC00005

PS00005 162->165 PKC_PHOSPHO_SITE PDOC00005

PS00005 189->192 PKC_PHOSPHO_SITE PDOC00005

PS00006 25->29 CK2_PHOSPHO_SITE PDOC00006

PS00006 80->84 CK2_PHOSPHO_SITE PDOC00006

PS00006 162->166 CK2_PHOSPHO_SITE PDOC00006

PS00006 218->222 CK2_PHOSPHO_SITE PDOC00006

PS00007 69->77 TYR_PHOSPHO_SITE PDOC00007

PS00008 70->76 MYRISTYL PDOC00008

PS00008 168->174 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_2gl8.2)

DKFZphfbr2_2hl

group: brain derived

DKFZphfbr2_2hl encodes a novel 180 amino acid protein with weak similarity to C. elegans D2007.4 protein

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . similarity to C. elegans D2007.4 protein

CpG island m 5' region, complete cDNA

Sequenced by Qiagen

Locus : unknown

Insert length: 957 bp

Poly A stretch at pos. 939, polyadenylation signal at pos. 916

1 GGGGGTCCCT GACTTTATAT GGCTGCTCCT GGCGAGCGAC TGAGTCGTCC

51 GTGAGGAAAA AGAGGCGAGG CTTTTCCGAG ATCGTCTCAG CGATGGCGCT

101 TCGGTCGCGG TTTTGGGGGT TGTTCTCGGT TTGCAGGAAC CCTGGGTGCA

151 GGTTCGCAGC CCTGTCAACC AGCTCCGAGC CGGCAGCGAA ACCTGAAGTG

201 GACCCTGTGG AAAATGAAGC TGTCGCCCCA GAATTCACCA ACCGGAACCC

251 CCGGAACCTG GAGCTTTTGT CTGTAGCCAG GAAAGAGCGG GGCTGGCGGA

301 CGGTGTTTCC CTCCCGTGAG TTCTGGCACA GGTTGCGAGT TATAAGGACT

351 CAGCATCATG TAGAAGCACT TGTGGAGCAT CAGAATGGCA AGGTTGTGGT

401 TTCGGCCTCC ACTCGTGAGT GGGCTATTAA AAAGCACCTT TATAGTACCA

451 GAAATGTGGT GGCTTGTGAG AGTATAGGAC GAGTGCTGGC ACAGAGATGC

501 TTAGAGGCGG GAATCAACTT CATGGTCTAC CAACCAACCC CGTGGGAGGC

551 AGCCTCAGAC TCGATGAAAC GACTACAAAG TGCCATGACA GAAGGTGGTG

601 TGGTTCTACG GGAACCTCAG AGAATCTATG AATAAATGGA AGCATTAATT

651 GTTTTGAACA TGTAAATATA AATCTGTCAG CCACTACAGC CATCAAAAGA

701 GAGCATCTGG AAGAACAGCC AGCTTGGAAG TTTTACAGCA ATAATGTTGC

751 AGTGGAATAT TATTTGTAGT TAAGGTCATC CTCCTCCCCT TTCTGTTTTT

801 TTAAATCAAG AACTACGTTC TGCCCCTCTC TTGGGCTTCA GAAGCATCTA

851 AGAAAAGCAG TCATCAATTA TAATTAACTT TCAAAGGGCA AGTCAGAAGT

901 TGTTTATAAA TTACAAAATA AAGGCATATT ATGAACTCTA AAAAAAAAAA

951 AAAAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 93 bp to 632 bp; peptide length: 16 Category: similarity to known protein Classif cation: unset

1 MALRSRFWGL FSVCRNPGCR FAALSTSSEP AAKPEVDPVE NEAVAPEFTN 51 RNPRNLELLS VARKERGWRT VFPΞREFWHR LRVIRTQHHV EALVEHQNGK 101 VVVSASTREW AIKKHLYSTR NVVACEΞIGR VLAQRCLEAG INFMVYQPTP 151 WEAASDSMKR LQSAMTEGGV VLREPQRIYE

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_2hl, frame 3 PIR:S44789 D2007.4 protein - Caenorhabditis elegans, N = 1, Score = 194, p = 2e-15

PIR:JC5753 ribosomal protein L18 - Vibrio proteolyticus, N = 1, Score = 121, P = 1. le-07

>PIR:S44789 D2007.4 protein - Caenorhabditis elegans Length = 170

HSPs:

Score = 194 (29.1 bits), Expect = 2.0e-15, P = 2.0e-15 Identities = 51/134 (38%), Positives = 78/134 (58%)

Query: 48 FTNRNPRNLELLSVARKERGWRTVFP—SREFWHRLRVIRTQHHVEA-LVEHQNGKVVVS 104

F NRNPRN EL+ G++ +R + +++ ++ + H E LV +Q+G VV+S Sbjct: 9 FVNRNPRNNELMGRQAPNTGYQFEKDRAARSYIYKVELVEGKSHREGRLVHYQDG-VVIS 67 Query: 105 ASTREWAIKKHLYSTRNVVACESIGRVLAQRCLEAGINFMVYQPTPWEAASDSMKRLQ— 162 AST+E +1 LYS + A +IGRVLA RCL++GI+F + T EA S + Sbjct: 68 ASTKEPSIASQLYSKTDTSAALNIGRVLALRCLQΞGIHFAMPGATK-EAIEKΞQHQTHFF 126 Query: 163 SAMTEGGVVLREPQRI 178

A+ E G+ L+EP + Sbjct: 127 KALEEEGLTLKEPAHV 142

Pedant information for DKFZphfbr2_2hl, frame 3

Report for DKFZphfbr2_2hl .3

[LENGTH] 180

[MW] 20576.57

[pi] 9.63

[HOMOL] PIR:S44789 D2007.4 protein - Caenorhabditis elegans 2e-13

[FUNCAT] j mrna translation and ribosome biogenesis [H. influenzae, HI0794] 2e-04

[SUPFAM] Escherichia col ribosomal protein L18 8e-06

[KW] Alpha_Beta

SEQ MALRSRFWGLFSVCRNPGCRFAALSTSSEPAAKPEVDPVENEAVAPEFTNRNPRNLELLS PRD ccccccceeeeeeeecccccceeeecccccccccccccccceeeecccccccccchhhhh

SEQ VARKERGWRTVFPSREFWHRLRVIRTQHHVEALVEHQNGKVVVSASTREWAIKKHLYSTR PRD hhhhcccccccchhhhhhhhhhccccchhhhhhhhhcccceeeeechhhhhhhhhhhhcc

SEQ NVVACESIGRVLAQRCLEAGINFMVYQPTPWEAASDSMKRLQSAMTEGGVVLREPQRIYE PRD ccceeehhhhhhhhhhhhhcceeeeeccccchhhhhhhhhhhhhhhccceeecccccccc

(No Prosite data available for DKFZphfbr2_2hl .3) (No Pfam data available for DKFZphfbr2_2hl .3)

DKFZphfbr2_2hlO

group: brain derived

DKFZphfbr2_2hlO encodes a novel 220 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of bram-specific genes . unknown complete cDNA, complete eds, EST hits

Sequenced by Qiagen

Locus : unknown

Insert length: 2176 bp

Poly A stretch at pos. 2161, polyadenylation signal at pos. 2143

1 TGGGGAGTAT TCTAATTATA TTTTATATTT AATAAATTAT TTTTCTATTT

51 CTTTGTTATA TTAAGTTGCA CACTTGTTTC TTTTATCCAG AAAGTTTAGT

101 ATAATAAAAA TAGTTTTAAG ATTAACTGTG AATGTAAAGG AAAAGTATTA

151 TTAATTATTT CAGGAAATTG CAAGACCTAA CATGGCTGAA AGAGAAACAG

201 AAACATCAAA TTCTGAAAGT AAACAAGATA AAGCTGCTTC TTCAAAAGAA

251 AAAAATGGAT GTAATGCAAA TTCATTTGAA GGCTCATCAA CAACAAAAAG

301 TGAAGAAAGC ATAACAGTTT CAGATAAGGA AAATGAAACC TGTCTTGCAG

351 ACCAGGAAAC TGGCTCAAAA AACATCGTCA GTTGTGATTC AAATATTGGT

401 GCAGATAAAG TGGAAAAGAA AAAACAAATA CAACACGTTT GTCAGGAAAT

451 GGAGTTGAAG ATGTGCCAGA GTTCAGAAAA CATAATCTTA TCTGATCAGA

501 TTAAAGATCA CAACTCCAGT GAAGCCAGAT TTTCTTCAAA GAATATTAAG

551 GATTTGCGAT TAGCATCAGA TAATGTAAGC ATTGATCAGT TTTTGAGAAA

601 AAGACATGAA CCTGAATCTG TTAGTTCTGA TGTTAGCGAG CAAGGCAGTA

651 TTCATTTGGA ACCTCTGACT CCATCCGAGG TACTTGAGTA TGAAGCCACA

701 GAGATTCTTC AGAAAGGTAG TGGTGATCCT TCAGCCAAGA CTGATGAAGT

751 AGTGTCTGAT CAAACAGATG ACATTCCTGG AGGAAATAAC CCTAGCACAA

801 CAGAGGCAAC AGTAGACCTG GAAG TGAAA AAGAAAGAAG TTGAAATTAG

851 TCATTTTAAG TTTCAGTGTA CCAACGATAA GGGCATTTGG AACAGTGCTA

901 TCAGGTGAGC TCAGTGGTGC TGTTGTAGGT TCAGAAATGG AAATATGTAA

951 GGGAGGTCAC ACATACACTT TACCTGTATG TTCAACCTAT GTTATCAAAC

1001 AAACCAATTC ACCAATAATA GCATGATTAG TAGGGATTCC CAAAAAGTTT

1051 TTAAAAACAC GAACAGGATT TTAATGATAA TTAAATTTGC AGTGGAAAGG

1101 TCTCATTTAA TGGTTTTCAA GGAAATGGGA TTTGGTTGCT GACATGAATT

1151 GATGATATTA GTAATATTTA TAAAGCCTTT CAAACTTCCA TCAATCCTAA

1201 GCTAAAAATC TTTATTACCT GTATATCCTT TTCAGTTAAC TGAGAGGAAG

1251 GGATTTGGAA ACCATGTACT TTTGGGGAGT AATTGATTAA AAACAATGGC

1301 TGATTGGCAT TGTTAATGAA GGCTTTATTT GTGAGGATGA TGCTGGTAAA

1351 TGGAGCATGC TTAGAGTACT AAATTGATCT AATGAGAATT TGGATGAACA

1401 TAAACTTAAT TTTGGATTTA ATATAACATT CCAGTCAGAC GCATGTAAAC

1451 AGAATATTTG AATCTTTGTA CCTCCATACA AGTGTTAGCC TGCCAGGCTG

1501 TAAGCTTACC TTAATTAAAC TTTCAGTGAA AGTGGAATTA TTAAGATATA

1551 AATTTATATT TGTGCTTTTT GTCAGTGTGT AAGCTGTGTA GAAATTCTTT

1601 GATGTATTAG TTGTATTAAT GTAAAGTAGA AACCCATTGT TGAAACTCCT

1651 GTAGCTATTA TGCTTTTAAT ATTGTTTTAA TGTTCTTCCT TAGAAATAGG

1701 CCCATAAAAA TGGTCTGGAA GCCAAACCAA AGTATGGTAT AATGTAGATA

1751 TTGTAAAGCA GTAAACTGAA AACATGTCCT GGCATGTATT CAGCCATGTT

1801 TAAGTGACTT TTCTGTAATT GTAAAATAAA AACTTCAAAT GGGACCTAAA

1851 ACAGTGATGT AAAAGAACTG GTTTTGGAAA TTTAGCCTAA TTTATCTATA

1901 AGATGGCTGC TAAATTGATT TTTCAGTTCT TTTTATCATC TAAAATATAA

1951 TAGATATAGA AATGAATAAT ATGAAGAACA GTAGTTTGCT TTGAAATACT

2001 AATAAACTTT TATTTAAGAT GCTTCATTTT TACTTCTTAA AACGTGCTTT

2051 GGATTCTTAA ATTTTGTTTC ACTGAATGTT CAATGTTTTA AATGGCGATT

2101 AAAATACTCT GCTGTATATA GTAGTTTTTG AGTAAATATT TGCAATAAAA

2151 ATCTGCCCCC GAAAAAAAAA AAAAAA

BLAST Results

Entry G35287 from database EMBL: human STS SHGC-37375. Score = 2163, P = 2.8e-91, identities 437/441 Medlme entries

No Medlme entry

Peptide information for frame 2

ORF from 182 bp to 841 bp; peptide length: 220 Category: putative protein

1 MAERETETSN SESKQDKAAS SKEKNGCNAN SFEGSSTTKS EESITVSDKE

51 NETCLADQET GSKNIVSCDS NIGADKVEKK KQIQHVCQEM ELKMCQSSEN

101 IILSDQIKDH NSSEARFSSK NIKDLRLASD NVSIDQFLRK RHEPESVSSD

151 VSEQGSIHLE PLTPSEVLEY EATEILQKGS GDPSAKTDEV VSDQTDDIPG

201 GNNPSTTEAT VDLEDEKERS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_2hlO, frame 2

No Alert BLASTP hits found

Pedant information for DKFZphfbr2_2hlO, frame 2

Report for DKFZphfbr2_2hlO .2

[LENGTH] 220

[MW] 24109.02

[pi] 4.51

[FUNCAT] 04.99 other transcription activities [S. cerevisiae, YKR092c] 4e-05

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKR092c] 4e-05

[PROSITE] MYRISTYL 3

[PROSITE] CK2_PHOSPHO_SITE 8

[PROSITE] PKC_PHOSPHO_SITE 5

[PROSITE] ASN_GLYCOSYLATION 3

[PFAM] TNFR/NGFR cysteine-rich region

[KW] Alpha_Beta

SEQ MAERETETSNSESKQDKAASSKEKNGCNANSFEGSSTTKSEESITVSDKENETCLADQET PRD cccccccccccccchhhhhhhhccccccccccccccccceeeeeeeeccccccccccccc

SEQ GSKNIVSCDSNIGADKVEKKKQIQHVCQEMELKMCQSSENIILSDQIKDHNSSEARFSΞK PRD cccceeeecccccchhhhhhhhhhhhhhhhhhhhhhccceeeeccccccccccccccccc

SEQ NIKDLRLASDNVSIDQFLRKRHEPESVΞSDVSEQGSIHLEPLTPSEVLEYEATEILQKGS PRD cchhhhhhcccchhhhhhhhcccccccccccccccceeecccccccchhhhhhhcccccc

SEQ GDPΞAKTDEVVSDQTDDIPGGNNPSTTEATVDLEDEKERS PRD ccccccccccccccccccccccccccceeeehhhhhhccc

Prosite for DKFZphfbr2_2hlO .2

PS00001 51->55 ASN_GLYCOSYLATION PDOC00001 PS00001 111->115 ASN_GLYCOSYLA ION PDOC00001 PS00001 131->135 ASN_GLYCOSYLATION PDOC00001 PS00005 20->23 PKC_PHOSPHO_SITE PDOC00005 PS00005 37->40 PKC_PHOSPHO_SITE PDOC00005 PS00005 47->50 PKC_PHOSPHO_SITE PDOC00005 PS00005 118->121 PKC_PHOSPHO_SITE PDOC00005 PS00005 184->187 PKC_PHOSPHO_SITE PDOC00005 PS00006 9->13 CK2_PHOSPHO_SITE PDOC00006 PS00006 13->17 CK2_PHOSPHO_SITE PDOC00006 PS00006 20->24 CK2_PHOSPHO_SITE PDOC00006 PS00006 38->42 CK2_PHOSPHO_SITE PDOC00006 PS00006 45->49 CK2_PHOSPHO_SITE PDOC00006 PS00006 47->51 CK2_PHOSPHO_SITE PDOC00006 PS00006 163->167 CK2_PHOSPHO_SITE PDOC00006 PS00006 205->209 CK2_PHOSPHO_SITE PDOC00006 PS00008 26->32 MYRISTYL PDOC00008 PS00008 34->40 MYRISTYL PDOC0000S

PS00008 201->207 MYRISTYL PDOCOOOOE

Pfam for DKFZphfbr2_2hlO .2

HMM_NAME TNFR/NGFR cysteine-rich region

HMM *CpeG. tYtD.WNHvpqClpCtrCePEMGQYMvqPCTwTQNTVC*

+E+ T +D +N ++C E G+ + +C+++ + Query 40 SEESITVSDKEN--ETC—LADQET—GSKNIVSCDSNIGADK 76

DKFZphfbr2 2ιl7

group: intracellular transport and trafficking

DKFZphfbr2_2ιl7.3 encodes a novel 201 amino acid putative GTP-b ding protein related to RablB.

Rab proteins are members of the Ras superfamily of GTPases. Rab proteins are localised to the cytoplasmic side of organelles and vesicles involved in the secretory (biosynthetic) and endocytotic pathways in eukaryotic cells. Rab proteins direct the targeting and fusion of transport vesicles to their acceptor membranes. RablB is essential for the intracellular transport of nascent low density lipoprotein (LDL) receptor. It is discussed as a universal mediator of endoplasmatic reticulum to Golgi transport of membrane glycoproteins in mammalian cells .

The new protein can find clinical application m modulating the transport of glycoproteins inside cells, especially of the LDL receptor.

Medline

96245776: Intracellular transport and maturation of nascent low density lipoprotein receptor is blocked by mutation in the Ras-related GTP-bmdmg protein, RAB1B strong similarity to rabl complete cDNA, complete eds, start at 47, EST hits

Sequenced by Qiagen

Locus : unknown

Insert length: 1985 bp

Poly A stretch at pos. 1901, polyadenylation signal at pos. 1859

1 GGGAGCAGAG TCGACTGGGA GCGACCGAGC GGGCCGCCGC CGCCGCCATG

51 AACCCCGAAT ATGACTACCT GTTTAAGCTG CTTTTGATTG GCGACTCAGG

101 CGTGGGCAAG TCATGCCTGC TCCTGCGGTT TGCTGATGAC ACGTACACAG

151 AGAGCTACAT CAGCACCATC GGGGTGGACT TCAAGATCCG AACCATCGAG

201 CTGGATGGCA AAACTATCAA ACTTCAGATC TGGGACACAG CGGGCCAGGA

251 ACGGTTCCGG ACCATCACTT CCAGCTACTA CCGGGGGGCT CATGGCATCA

301 TCGTGGTGTA TGACGTCACT GACCAGGAAT CCTACGCCAA CGTGAAGCAG

351 TGGCTGCAGG AGATTGACCG CTATGCCAGC GAGAACGTCA ATAAGCTCCT

401 GGTGGGCAAC AAGAGCGACC TCACCACCAA GAAGGTGGTG GACAACACCA

451 CAGCCAAGGA GTTTGCAGAC TCTCTGGGCA TCCCCTTCTT GGAGACGAGC

501 GCCAAGAATG CCACCAATGT CGAGCAGGCG TTCATGACCA TGGCTGCTGA

551 AATCAAAAAG CGGATGGGGC CTGGAGCAGC CTCTGGGGGC GAGCGGCCCA

601 ATCTCAAGAT CGACAGCACC CCTGTAAAGC CGGCTGGCGG TGGCTGTTGC

651 TAGGAGGGGC ACATGGAGTG GGACAGGAGG GGGCACCTTC TCCAGATGAT

701 GTCCCTGGAG GGGGGAGGAG GTACCTCCCT CTCCCTCTCC TGGGGCATTT

751 GAGTCTGTGG CTTTGGGGTG TCCTGGGCTC CCCATCTCCT TCTGGCCCAT

801 CTGCCTGCTG CCCTGAGCCC CGGTTCTGTC AGGGTCCCTA AGGGAGGACA

851 CTCAGGGCCT GTGGCCAGGC AGGGCGGAGG CCTGCTGTGC AGTTGCCTCT

901 AGGTGACTTT CCAAGATGCC CCCCTACACA CCTTTCTTTG GAACGAGGGC

951 TCTTCTGTCG GTGTCCCTCC CACCCCCATG TATGCTGCAC TGGGTTCTCT

1001 CCTTCTTCTT CCTGCTGTCC TGCCCAAGAA CTGAGGGTCT CCCCGGCCTC

1051 TACTGCCCTG GCTGCAGTCA GTGCCCAGGG CGAGGAATGT GGCCAGGGGA

1101 TCCAGGACCT GGGATCCAGG GCCCTGGGCT GGACCTCAGG ACAGGCATGG

1151 AGGCCACAGG GGCCCAGCAG CCCACCCTTT CCTCTCCCCA CTGCCTCCTC

1201 TCCCTTCCTA CACTCCCAGC TCGAGCCGTC CAGCTGCGGT GGGATCTGAG

1251 TATATCTAGG GCGGGTGGGC GGGTAGCAGT GCTGGGCCTG TGTCTTGAGC

1301 CTGGAGGGAG ACTGCTCCTG CCGCCCTCTG CCCTGCCGGA GACAGACCCA

1351 TGCGCTGCCT GCCCACCGTG CCCCTTTGTC CCCATGTCAG GCGGAGGCGG

1401 AAGGCCCACC GTGCCAGAGG CTGGGCACCA GCCTTAACCC TCACTCTGCT

1451 AGCACCTCCT CCCTTTCCCC AAGGTAGCAC ATCTGGCTCA CTCCCCACTC

1501 CGTCTCTGGA GCCCACCAGG GAAGGCCCTC ATCCCCTGCC GCTACTTCTC

1551 TGGGGAATGT GGGTTCCATC CAGGATTGGG GGCCTCTCTG CTCACCCACT

1601 CTGCACCCAG GATCCTAGTC CCCTGCCCTC TGGCACAGCT GCTTCCTGCA

1651 AGAAAGCAAG TCTTTGGTCT CCCTGAGAAG CCATGTCCCT CGTGCTGTCT

1701 CTTGCCTGTC CCACCTGTGC CCTGCCCTCC AGCTTGTATT TAAGTCCCTG

1751 GGCTGCCCCC TTGGGGTGCC CCCCGCTCCC AGGTTCCCCT CTGGTGTCAT

1801 GTCAGGCATT TTGCAAGGAA AAGCCACTTG GGGAAAGATG GAAAAGGACA

1851 AAAAAAATTA ATAAATTTCC ATTGGCCCTC GGGTGAGCTG AGGGTTTTTG

1901 CAAGGAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

1951 AAAAAAAAAA AAAAGAAAAA AAAAAAAAAA AAAAA BLAST Results

No BLAST result

Medline entries

91115900:

A family of ras-like GTP-binding proteins expressed in electromotor neurons .

Peptide information for frame 3

ORF from 48 bp to 650 bp; peptide length: 201 Category: strong similarity to known protein

1 MNPEYDYLFK LLLIGDSGVG KSCLLLRFAD DTYTESYIST IGVDFKIRTI

51 ELDGKTIKLQ IWDTAGQERF RTITSSYYRG AHGIIVVYDV TDQESYANVK

101 QWLQEIDRYA SENVNKLLVG NKSDLTTKKV VDNTTAKEFA DSLGIPFLET

151 SAKNATNVEQ AFMTMAAEIK KRMGPGAASG GERPNLKIDS TPVKPAGGGC

201 C

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_2ιl7, frame 3

SWISSPROT:RBlB_RAT RAS-RELATED PROTEIN RAB-1B., N = 1, Score = 1023, P = 2.7e-103

PIR:S06147 GTP-binding protein rablB - rat, N = 1, Score = 1013, P = 3.2e-102

SWISSPROT:RABl_DISOM RAS-RELATED PROTEIN ORAB-1. , N = 1, Score = 967, P = 2.4e-97

PIR:TVHUYP GTP-bind g protein Rabl - human, N = 1, Score = 966, P = 3e-97

>SWISΞPROT:RBlB_RAT RAS-RELATED PROTEIN RAB-1B. Length = 201

HSPs:

Score = 1023 (153.5 bits), Expect = 2.7e-103, P = 2.7e-103 Identities = 197/201 (98%), Positives = 199/201 (99%)

Query: 1 MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQ 60

MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQ Sbjct: 1 MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYIΞTIGVDFKIRTIELDGKTIKLQ 60

Query: 61 IWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVG 120

IWDTAGQERFRT+TSSYYRGAHGIIVVYDVTDQEΞYANVKQWLQEIDRYASENVNKLLVG Sbjct: 61 IWDTAGQERFRTVTSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVG 120

Query: 121 NKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASG 180

NKSDLTTKKVVDNTTAKEFADSLG+PFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASG Sbjct: 121 NKSDLTTKKVVDNTTAKEFADSLGVPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASG 180

Query: 181 GERPNLKIDSTPVKPAGGGCC 201

GERPNLKIDSTPVK A GGCC Sbjct: 181 GERPNLKIDSTPVKSASGGCC 201

Pedant information for DKFZphfbr2_2ιl7, frame 3

Report for DKFZphfbr2_2ιl7.3

[LENGTH] 201 [MW] 22171.25

[pi] 5.56

[HOMOL] SWISSPR0T:RB1B_RAT RAS-RELATED PROTEIN RAB-1B. le-112

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YFL038c]

2e-77

[FUNCAT] 30.08 organization of golgi [S. cerevisiae, YFL038c] 2e-77

[FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae,

YFL005w] 4e-57

[FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YFL005w] 4e-57

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YFL005w]

4e-57

[FUNCAT] 08.19 cellular import [S. cerevisiae, YER031c] 8e-46

[FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YER031c] 8e-46

[FUNCAT] 09.09 biogenesis of intracellular transport vesicles [S. cerevisiae,

YGL210w] le-44

[FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YOR089c] le-30

[FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YNL098c] 3e-25

[FUNCAT] 11.01 stress response [Ξ. cerevisiae, YNL098c] 3e-25

[FUNCAT] 03.99 other cell growth, cell division and dna synthesis activities [S. cerevisiae YNL098c] 3e-25

[FUNCAT] 01.03.13 regulation of nucleotide metabolism [Ξ. cerevisiae, YNL098C]

3e-25

[FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YNL098c]

3e-25

[FUNCAT] 10.04.07 g-proteins [S. cerevisiae, YNL098c] 3e-25

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YNL098c] 3e-25

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YORlOlw] 9e-24

[FUNCAT] 11.10 cell death [S. cerevisiae, YORlOlw] 9e-24

[FUNCAT] 04.07 rna transport [S. cerevisiae, YOR185c] 4e-23

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YOR185c] 4e-23

[FUNCAT] 08.01 nuclear transport [S. cerevisiae, YOR185c] 4e-23

[FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YPR165w] 7e-17

[FUNCAT] 10.02.07 g-proteins [S. cerevisiae, YPR165w] le-11

[FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YCR027c] le-16

[FUNCAT] 03.07 pheromone response, matmg-type determination, sex-specific proteins

[S. cerevisiae, YLR229c] le-11 [FUNCAT] 10.05.07 g-proteins [S. cerevisiae, YLR229c] le-11 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YDL192w] 4e-10 [FUNCAT] 03.01 cell growth [S. cerevisiae, YNL180c] 9e-09 [FUNCAT] 06.07 protein modification (glycolsylation, acylation, myristylation, palmitylat on, farnesylation and processing) [S. cerevisiae, YPL051w] 3e-08 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YAL048c] 5e-05 [BLOCKS] BL01019A ADP-ribosylation factors family proteins [BLOCKS] BL01115A GTP-binding nuclear protein ran proteins [SCOP] dlplk 3.25.1.3.1 cH-p21 Ras protein [human (Homo sapiens) 2e-41 [SCOP] dlguaa_ 3.25.1.3.10 RaplA [Human (Homo sapiens) 5e-60 [SCOP] dlrrga_ 3.25.1.3.5 ADP-ribosylation factor 1 (ARFl) [rat (Rattu 2e-30 [SCOP] dlhura_ 3.25.1.3.4 ADP-ribosylation factor 1 (ARFl) [human (Horn 2e-33 [PIRKW] nucleus le-21 [PIRKW] membrane trafficking le-110 [PIRKW] oncogene le-25 [PIRKW] endoplasmic reticulum le-105 [PIRKW] phosphoprotein le-105 [PIRKW] glycoprotein 3e-25 [PIRKW] prenylated cysteine le-110 [PIRKW] signal transduction 4e-23 [PIRKW] transforming protein le-105 [PIRKW] purme nucleotide binding 2e-24 [PIRKW] alternative splicing 5e-26 [PIRKW] P-loop le-110 [PIRKW] lipoprotein le-110 [PIRKW] proto-oncogene 3e-27 [PIRKW] methylated carboxyl end 3e-27 [PIRKW] hydrolase 7e-25 [PIRKW] membrane protein le-105 [PIRKW] GTP binding le-110 [PIRKW] thiolester bond 5e-76 [PIRKW] Golgi apparatus le-105 [SUPFAM] ras transforming protein le-110 [PROSITE] ATP_GTP_A 1 [PROSITE] MYRISTYL 2 [PROSITE] CK2_PHOSPHO_SITE 5 [PROSITE] SIGMA54_INTERACT_1 1 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 4 [PROSITE] ASN_GLYCOSYLATION 3 [PFAM] Ras family (contains ATP/GTP binding P-loop) [KW] Alpha_Beta [KW] 3D SEQ MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQ

22 lp- EEEEEEETTTTCHHHHHHHHHHCCCCCCCCCTTTEEEE-EEEEETTEEEEEE

SEQ IWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVG

22 lp- EEECTTTTTTCGGGHHHHHHCCEEEEEEETTBHHHHHHHHHHHHHHHHHHTTTTCEEEEE

SEQ NKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASG

22 lp- ETTTTCCC-CCCHHHHHHHHHHCCCCEEEETTTTTTTHHHHHHHHHHHHHH

SEQ GERPNLKIDSTPVKPAGGGCC

221p-

Prosite for DKFZphfbr2_2ιl7.3

PS00001 121->125 ASN_GLYCOSYLATION PDOC00001

PS00001 133->137 ASN_GLYCOSYLATION PDOC00001

PS00001 154->158 ASN__GLYCOSYLATION PDOC00001

PS00002 17->21 GLYCOSAMINOGLYCAN PDOC00002

PS00005 56->59 PKC_PHOSPHO_SITE PDOC00005

PS00005 126->129 PKC_PHOSPHO_SITE PDOC00005

PS00005 135->138 PKC_PHOSPHO_SITE PDOC00005

PS00005 151-M54 PKC_PHOSPHO_SITE PDOC00005

PS00006 32->36 CK2_PHOSPHO_SITE PDOC00006

PS00006 91->95 CK2_PHOSPHO_SITE PDOC00006

PS00006 135->139 CK2_PHOSPHO_SITE PDOC00006

PS00006 156->160 CK2_PHOSPHO_SITE PDOC00006

PS00006 179->183 CK2_PHOSPHO_SITE PDOC00006

PS00007 27->34 TYR_PHOSPHO_SITE PDOC00007

PS00008 18->24 MYRISTYL PDOC00008

PS00008 176->182 MYRISTYL PDOC00008

PS00017 15->23 ATP_GTP_A PDOC00017

PS00675 ll->25 SIGMA54 INTERACT 1 PDOC00579

Pfam for DKFZphfbr2_2ιl7.3

HMM_NAME Ras family (contains ATP/GTP binding P-loop)

HMM *KLVLIGDSGVGKSCLLIRFTQNeFnEeYI PTIGvDFYtKTIEIDGKtIK KL+LIGDSGVGKSCLL+RF +++++E+YI+TIGVDF+++TIE+DGKTIK

Query 10 KLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIK 58

HMM LQIWDTAGQERYRsMRPMYYRGAMGFMLVYDITNRqΞFENIrNWweEIrR LQIWDTAGQER+R+++++YYRGA+G+++VYD+T+++S+ N+++W++EI+R

Query 59 LQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDR 108

HMM HCDrDENVPIMLVGNKCDLEDQRQVStEEGQeFAREWGAIPFMETSAKTN +++ ENV ++LVGNK+DL +++V+ +++EFA+++G IPF+ETSAK++

Query 109 YAS—ENVNKLLVGNKSDLTTKKVVDNTTAKEFADSLG-IPFLETSAKNA 155

HMM iNVEEAFMEIvRellqrMqe.q.NqteNinidQpsrnrk... rCCCIM* +NVE+AFM+++ EI++RM+ +++E +N++ +S++ K +CC

Query 156 TNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPAGGGCC-- 201

DKFZphfbr2_2kl9

group: brain derived

DKFZphfbr2_2kl9 encodes a novel 303 amino acid protein with similarity to human KIAA0378 product .

The protein contains a leucine zipper, which can mediate protem-protein-interaction. No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can f nd application in studying the expression profile of brain-specific genes . similarity to KIAA0378 encoded by the genomic clones HS147M19/HS608E8

Sequenced by Qiagen

Locus: unknown

Insert length: 1931 bp

Poly A stretch at pos. 1866, no polyadenylation signal found

1 GGGGGGGGCG CGCGGTGACA GCGCGGGGTT GGCGGCGTGG GACCCAGGGG

51 GCGACAGAGG CAGCAGCAGC CCGAGGCCTG AGGAGAGGAG ACCGGCGGCG

101 GCGGCAATGC TGGAGACCCT TCGCGAGCGG CTGCTGAGCG TGCAGCAGGA

151 TTTCACCTCC GGGCTGAAGA CTTTAAGTGA CAAGTCAAGA GAAGCAAAAG

201 TGAAAAGCAA ACCCAGGACT GTTCCATTTT TGCCAAAGTA CTCTGCTGGA

251 TTAGAATTAC TTAGCAGGTA TGAGGATACA TGGGCTGCAC TTCACAGAAG

301 AGCCAAAGAC TGTGCAAGTG CTGGAGAGCT GGTGGATAGC GAGGTGGTCA

351 TGCTTTCTGC GCACTGGGAG AAGAAAAAGA CAAGCCTCGT GGAGCTGCAA

401 GAGCAGCTCC AGCAGCTCCC AGCTTTAATC GCAGACTTAG AATCCATGAC

451 AGCAAATCTG ACTCATTTAG AGGCGAGTTT TGAGGAGGTA GAGAACAACC

501 TGCTGCATCT GGAAGACTTA TGTGGGCAGT GTGAATTAGA AAGATGCAAA

551 CATATGCAGT CCCAGCAACT GGAGAATTAC AAGAAAAATA AGAGGAAGGA

601 ACTTGAAACC TTCAAAGCTG AACTAGATGC AGAGCACGCC CAGAAGGTCC

651 TGGAAATGGA GCACACCCAG CAAATGAAGC TGAAGGAGCG GCAGAAGTTT

701 TTTGAGGAAG CCTTCCAGCA GGACATGGAG CAGTACCTGT CCACTGGCTA

751 CCTGCAGATT GCAGAGCGGC GAGAGCCCAT AGGCAGCATG TCATCCATGG

801 AAGTGAACGT GGACATGCTG GAGCAGATGG TCCTGATGGA CATATCGGAC

851 CAGGAGGCCC TGGACGTCTT CCTGAACTCT GGAGGAGAAG AGAACACTGT

901 GCTGTCCCCC GCCTTAGGTA GGGTTGACAA ACTTGCATTA GCTGAACCAG

951 GGCAGTATCG ATGCCACTCC CCTCCAAAGG TGAGACGTGA GAACCATCTG

1001 CCAGTCACTT ACGCATAAAC CCCCAAGCTC ACAGCCAGCT CCTGGCTCCC

1051 TAACCCCACG GTTCCACACG GCTGTGTGGC AGCTGCAACA GTGGTGTGGT

1101 TCCGTCATGA ATTCTTCTCA AAGATTTGAC ATGCTCCACT CCGGTAACTT

1151 TGGTGAGTTG AGAGCTTTCT TGTTTGTTTT CCCTCCTTTA CCATCCAGAA

1201 ATCCATTTGA GTCTGCTCCT TGTGGTTAAG GACTGGCGTT TGCAGGGAGG

1251 TGCGGACTCT CCTGCGGGGC TCACGGGAAA CTCTTCCCTC TTCGTGCGAC

1301 AGGCATTTAG GGGCGTGCCT GCCATGGGCA AAGCCATGGT GTGTGTTCAG

1351 CTCTTGGCCT GTGTTGTAAA CTTAGTTGCA CTTCAGTTCC TTTCATCCCT

1401 TCACAAAATT TTGTTTCACA TTCATGCAGC AAATATGGGC TGAGGTGCCA

1451 GACCTGTACC TGGGCTTGGT GCGTTTCAAA TTTCAGACCA GTTCTTTGGG

1501 CTGGGTCAAG GCAAAGCTCA GTCGTCCCAG CAGCACCTCA GCCATCTGTA

1551 GAAGGTTCTA CCATTACCAC GGTTTCAGCT TCCTCTAAAC TTCTCACCCG

1601 CTTCTCCTGG CAATCTGTCA GAACGGTGTC ATCCTGGGGA AGAGAAGGAG

1651 CTTGGGTGCA TTTGCCCTCA TCCTGAGAAG GCCAGAATAC TGGAGACCAG

1701 CGTGAACCCT CACCCAGAGT CAGGGGAAGA TTTAGAAACA GTGACACCTG

1751 CATATAGAAT TTTGATTCCT TGAAGAGCCT ATTTAGTTCC ATAAAATTGG

1801 AGAACTGCTG AAGGTCAGTA ATTCCGACTT TCTCAGCAGT GGTGTCTCTG

1851 AATTACTGCA AAGGGTAAAA AAAAAAAAAA AAAAAACTTA TCGATACCGT

1901 CGACCTCGAT GATGATGATG ATGATGTCGA C

BLAST Results

Entry HS147M19 from database EMBL:

Homo sapiens DNA sequence from PAC 147M19 on chromosome 6p22.1-22.3.

Contains an unknown gene, ESTs and GSSs.

Score = 5540, P = 4.1e-275, identities = 1114/1120

3 exons 592-1884

Entry HS608E8 from database EMBL:

Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone 608E8

Score = 797, P = 1.2e-78, identities = 161/163 6 exons 1-592

Medline entries

90294724:

The involucrin gene of the gibbon: The middle region shared by the hominoids

Peptide information for frame 2

ORF from 107 bp to 1015 bp; peptide length: 303 Category: similarity to known protein Classification: unset Prosite motifs: LEUCINE ZIPPER (97-119)

1 MLETLRERLL SVQQDFTSGL KTLSDKSREA KVKSKPRTVP FLPKYSAGLE

51 LLSRYEDTWA ALHRRAKDCA SAGELVDSEV VMLSAHWEKK KTSLVELQEQ

101 LQQLPALIAD LESMTANLTH LEASFEEVEN NLLHLEDLCG QCELERCKHM

151 QSQQLENYKK NKRKELETFK AELDAEHAQK VLEMEHTQQM KLKERQKFFE

201 EAFQQDMEQY LSTGYLQIAE RREPIGSMSS MEVNVDMLEQ MVLMDISDQE

251 ALDVFLNSGG EENTVLSPAL GRVDKLALAE PGQYRCHSPP KVRRENHLPV

301 TYA

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_2kl9, frame 2

TREMBL :HSAB2376_1 gene: "KIAA0378"; Human mRNA for KIAA0378 gene, partial eds., N = 1, Score = 137, P = 4.8e-06

PIR: 137037 involucrin - common gibbon, N = 1, Score = 124, P = 7.4e-05

PIR:A57013 early endosome antigen 1 - human, N = 1, Score = 128, P = 9.5e-05

>TREMBL:HSAB2376_1 gene: "KIAA0378"; Human mRNA for KIAA0378 gene, partial eds .

Length = 808

HSPs:

Score = 137 (20.6 bits), Expect = 4.8e-06, P = 4.8e-06 Identities = 59/222 (26%), Positives = 103/222 (46%)

Query: 2 LETLRERLLΞVQQDFTSGLKTL SDKSREAKVKS-KPRTVPFLPKYSAGLELLSRYED 57

L TL E L S ++ LK D+ R +++S + K +A L+ E Sbjct: 434 LATLEEAL-SEKERIIERLKEQRERDDRERLEEIESFRKENKDLKEKVNALQAELTEKES 492

Query: 58 TWAALHRRAKDCASAGELVDSEVVMLSAHWEKKKTSLVELQEQLQQLPALIADLESMTAN 117

+ L A ASAG DS++ L E+KK +L+ QL++ I D M Sbjct: 493 SLIDLKEHASSLASAGLKRDSKLKSLEIAIEQKKEECSKLEAQLKKAHN-IEDDSRMNPE 551

Query: 118 LTHLEASFEEVENNLLHLEDLCG—QCELERCKHMQSQQLENYKKNKRK ELETFKAE 172

++++ + D CG Q E++R + +++EN K +K K ELE+ Sbjct: 552 FAD QIKQLDKEASYYRDECGKAQAEVDRLLEIL-KEVENEKNDKDKKIAELESLTLR 607

Query: 173 LDAEHAQKVLEMEHTQQMKLKERQKFFEEAFQQDMEQYLSTGYLQIAE 220

+ +KV ++H QQ++ K+ + EE +++ ++ +LQI E Sbjct: 608 HMKDQNKKVANLKHNQQLEKKKNAQLLEEVRRREDSMADNSQHLQIEE 655

Score = 100 (15.0 bits), Expect = 6.2e-02, P = 6.0e-02 Identities = 44/156 (28%), Positives = 76/156 (48%)

Query: 57 DTWAALHRRAKDCASAGELVDSEVVMLSAHWEKKKTSLVELQEQLQQLPAL-IADLESMT 115

D A+ +R +C A VD + +L E +K + +L+ L + D Sbjct: 560 DKEASYYR—DECGKAQAEVDRLLEILK-EVENEKNDKDKKIAELESLTLRHMKDQNKKV 616

Query: 116 ANLTHLEASFEEVENNLLHLEDLCGQCE—LERCKHMQSQQLENYKKNKRKELETFKAEL 173 ANL H + E+ +N L LE++ + + + +H+Q ++L N + R+EL+ KA L Sbjct: 617 ANLKHNQ-QLEKKKNAQL-LEEVRRREDSMADNSQHLQIEELMNALEKTRQELDATKARL 674

Query: 174 DAEHAQKVLEME-HTQQMKLKERQKFFEEAFQQDMEQYLS 212

A Q + E E H +++ ER+K EE + E L+ Sbjct: 675 -ASTQQSLAEKEAHLANLRI-ERRKQLEEILEMKQEALLA 712

Pedant information for DKFZphfbr2_2kl9, frame 2

Report for DKFZphfbr2_2kl9.2

[LENGTH] 303

[MW] 34814.78

[pi] 5.23

[PROSITE] LEUCINE ZIPPER 1

[KW] All Alpha

[KW] LOW COMPLEXITY 3.63 %

[KW] COILED COIL 14.52 %

SEQ MLETLRERLLSVQQDFTSGLKTLSDKSREAKVKΞKPRTVPFLPKYSAGLELLSRYEDTWA SEG PRD ccchhhhhhhhhhhccccccchhhhhhhhhhhhccccccccccchhhhhhhhhhhhchhh COILS

SEQ ALHRRAKDCASAGELVDSEVVMLSAHWEKKKTSLVELQEQLQQLPALIADLESMTANLTH SEG xxxxxxxxxxx PRD hhhhhhhhchhhhhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCC

SEQ LEASFEEVENNLLHLEDLCGQCELERCKHMQSQQLENYKKNKRKELETFKAELDAEHAQK SEG PRD hhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCC

SEQ VLEMEHTQQMKLKERQKFFEEAFQQDMEQYLSTGYLQIAERREPIGSMSSMEVNVDMLEQ SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcchhhhhhhcccccccccchhhhhhhhh COILS

SEQ MVLMDISDQEALDVFLNSGGEENTVLSPALGRVDKLALAEPGQYRCHSPPKVRRENHLPV SEG PRD hhhhhhchhhhhhhhhccccccceeeccccccccceeeccccccccccccceeecccccc COILS

SEQ TYA SEG PRD COILS

Prosite for DKFZphfbr2_2kl9.2

PS00029 97->119 LEUCINE ZIPPER PDOC00029

(No Pfam data available for DKFZphfbr2_2kl9.2)

DKFZphfbr2_2kl4

group: cell cycle

DKFZphfbr2_2kl4 encodes a novel 335 ammo acid protein with strong similarity to rattus rattus IAG2 "implantation-associated protein" and the human N33 tumour-suppressor gene.

Tumour-suppressor genes are known to be involved m the control of cell growth and division, interacting with proteins which control the cell cycle. The N33 gene is significantly methylated in tumour cells, a mechanism by which tumor-suppressor genes are inactivated in cancer. In addition, the novel protein contains a RGD cell attachment site. Therefore the novel protein is a new putative tumour-suppressor gene.

The new protein can find application in modulating/blocking the cell cycle and in the therapy of tumours. strong similarity to human N33 tumor suppressor gene complete cDNA, complete eds, EST hits, potential start at Bp 30 matches kozak consensus ANCatgG potential transmembran protein (4 TM) similarity to yeast OST3p (oligosaccharyltransferase gamma chain)

Sequenced by Qiagen

Locus: unknown

Insert length: 2241 bp

Poly A stretch at pos. 2221, no polyadenylation signal found

1 TGGGACTTAT AGAAGGGAGA GGAGCGAACA TGGCAGCGCG TTGGCGGTTT 51 TGGTGTGTCT CTGTGACCAT GGTGGTGGCG CTGCTCATCG TTTGCGACGT

101 TCCCTCAGCC TCTGCCCAAA GAAAGAAGGA GATGGTGTTA TCAGAAAAGG

151 TTAGTCAGCT GATGGAATGG ACTAACAAAA GACCTGTAAT AAGAATGAAT

201 GGAGACAAGT TCCGTCGCCT TGTGAAAGCC CCACCGAGAA ATTACTCCGT

251 TATCGTCATG TTCACTGCTC TCCAACTGCA TAGACAGTGT GTCGTTTGCA

301 AGCAAGCTGA TGAAGAATTC CAGATCCTGG CAAACTCCTG GCGATACTCC

351 AGTGCATTCA CCAACAGGAT ATTTTTTGCC ATGGTGGATT TTGATGAAGG

401 CTCTGATGTA TTTCAGATGC TAAACATGAA TTCAGCTCCA ACTTTCATCA

451 ACTTTCCTGC AAAAGGGAAA CCCAAACGGG GTGATACATA TGAGTTACAG

501 GTGCGGGGTT TTTCAGCTGA GCAGATTGCC CGGTGGATCG CCGACAGAAC

551 TGATGTCAAT ATTAGAGTGA TTAGACCCCC AAATTATGCT GGTCCCCTTA

601 TGTTGGGATT GCTTTTGGCT GTTATTGGTG GACTTGTGTA TCTTCGAAGA

651 AGTAATATGG AATTTCTCTT TAATAAAACT GGATGGGCTT TTGCAGCTTT

701 GTGTTTTGTG CTTGCTATGA CATCTGGTCA AATGTGGAAC CATATAAGAG

751 GACCACCATA TGCCCATAAG AATCCCCACA CGGGACATGT GAATTATATC

801 CATGGAAGCA GTCAAGCCCA GTTTGTAGCT GAAACACACA TTGTTCTTCT

851 GTTTAATGGT GGAGTTACCT TAGGAATGGT GCTTTTGTGT GAAGCTGCTA

901 CCTCTGACAT GGATATTGGA AAGCGAAAGA TAATGTGTGT GGCTGGTATT

951 GGACTTGTTG TATTATTCTT CAGTTGGATG CTCTCTATTT TTAGATCTAA 1001 ATATCATGGC TACCCATACA GCTTTCTGAT GAGTTAAAAA GGTCCCAGAG 1051 ATATATAGAC ACTGGAGTAC TGGAAATTGA AAAACGAAAA TCGTGTGTGT 1101 TTGAAAAGAA GAATGCAACT TGTATATTCT GTATTACCTC TTTTTTTCAA 1151 GTGATTTAAA TAGTTAATCA TTTAACCAAA GAAGATGTGT AGTGCCTTAA 1201 CAAGCAATCC TCTGTCAAAA TCTGAGGTAT TTGAAAATAA TTATCCTCTT 1251 AACCTTCTCT TCCCAGTGAA CTTTATGGAA CATTTAATTT AGTACAATTA 1301 AGTATATTAT AAAAATTGTA AAACTACTAC TTTGTTTTAG TTAGAACAAA 1351 GCTCAAAACT ACTTTAGTTA ACTTGGTCAT CTGATCTTAT ATTGCCTTAT 1401 CCAAAGATGG GGAAAGTAAG TCCTGACCAG GTGTTCCCAC ATATGCCTGT 1451 TACAGATAAC TACATTAGGA ATTCATTCTT AGCTTCTTCA TCTTTGTGTG 1501 GATGTGTATA CTTTACGCAT CTTTCCTTTT GAGTAGAGAA ATTATGTGTG 1551 TCATGTGGTC TTCTGAAAAT GGAACACCAT TCTTCAGAGC ACACGTCTAG 1601 CCCTCAGCAA GACAGTTGTT TCTCCTCCTC CTTGCATATT TCCTACTGCG 1651 CTCCAGCCTG AGTGATAGAG TGAGACTCTG TCTCAAAAAA AAAGTATCTC 1701 TAAATACAGG ATTATAATTT CTGCTTGAGT ATGGTGTTAA CTACCTTGTA 1751 TTTAGAAAGA TTTCAGATTC ATTCCATCTC CTTAGTTTTC TTTTAAGGTG 1801 ACCCATCTGT GATAAAAATA TAGCTTAGTG CTAAAATCAG TGTAACTTAT 1851 ACATGGCCTA AAATGTTTCT ACAAATTAGA GTTTGTCACT TATTCCATTT 1901 GTACCTAAGA GAAAAATAGG CTCAGTTAGA AAAGGACTCC CTGGCCAGGC 1951 GCAGTGACTT ACGCCTGTAA TCTCAGCACT TTGGGAGGCC AAGGCAGGCA 2001 GATCACGAGG TCAGGAGTTC GAGACCATCC TGGCCAACAT GGTGAAACCC 2051 CGTCTCTACT AAAAATATAA AAATTAGCTG GGTGTGGTGG CAGGAGCCTG 2101 TAATCCCAGC TGCACAGGAG GCTGAGGCAC GAGAATCACT TGAACTCAGG 2151 AGATGGAGGT TTCAGTGAGC CGAGATCACG CCACTGCACT CCAGCCTGGC 2201 AACAGAGCGA GACTCCATCT CAAAAAAAAA AAAAAAAAAA A BLAST Results

No BLAST result

Medline entries

96299740:

Structure and methylation-associated silencing of a gene within a homozygously deleted region of human chromosome band 8p22.

97243398:

Tumour-suppressor genes in prostatic oncogenesis: a positional approach.

98334474:

Concordant methylation of the ER and N33 genes in glioblastoma multiforme.

Peptide information for frame 3

ORF from 30 bp to 1034 bp; peptide length: 335 Category: strong similarity to known protein

1 MAARWRFWCV SVTMVVALLI VCDVPSASAQ RKKEMVLSEK VSQLMEWTNK

51 RPVIRMNGDK FRRLVKAPPR NYSVIVMFTA LQLHRQCVVC KQADEEFQIL

101 ANSWRYSSAF TNRIFFAMVD FDEGSDVFQM LNMNSAPTFI NFPAKGKPKR

151 GDTYELQVRG FSAEQIARWI ADRTDVNIRV IRPPNYAGPL MLGLLLAVIG

201 GLVYLRRSNM EFLFNKTGWA FAALCFVLAM TSGQMWNHIR GPPYAHKNPH

251 TGHVNYIHGS SQAQFVAETH IVLLFNGGVT LGMVLLCEAA TSDMDIGKRK

301 IMCVAGIGLV VLFFSWMLSI FRSKYHGYPY SFLMS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_2kl4, frame 3

TREMBL:RNAF855 _1 gene: "IAG2"; product: "implantation-associated protein"; Rattus norvegicus implantation-associated protein (IAG2) mRNA, partial eds., N = 1, Score = 1560, P = 3.4e-160

PIR:G02297 gene N33 protein - human, N = 1, Score = 1256, P = 5.6e-128

TREMBL:HSN33S11_1 gene: "N33"; product: "N33 protein form 2"; Human N33 protein form 2 (N33) gene, exon 11 and complete eds., N = 1, Score = 1252, P = 1.5e-127

>TREMBL:RNAF8554_1 gene: "IAG2"; product: "implantation-associated protein";

Rattus norvegicus implantation-associated protein (IAG2) mRNA, partial eds. Length = 308

HSPs:

Score = 1560 (234.1 bits), Expect = 3.4e-160, P = 3.4e-160 Identities = 295/307 (96%), Positives = 299/307 (97%)

Query: 29 AQRKKEMVLSEKVSQLMEWTNKRPVIRMNGDKFRRLVKAPPRNYSVIVMFTALQLHRQCV 88

AQRKKE VL EKV QLMEWTN+RPVIRMNGDKFR LVKAPPRNYSVIVMFTALQLHRQCV Sbjct: 2 AQRKKEKVLVEKVIQLMEWTNQRPVIRMNGDKFRPLVKAPPRNYSVIVMFTALQLHRQCV 61

Query: 89 VCKQADEEFQILANSWRYSSAFTNRIFFAMVDFDEGSDVFQMLNMNΞAPTFINFPAKGKP 148

VCKQADEEFQILAN WRYSSAFTNRIFFAMVDFDEGSDVFQMLNMNSAPTFINFP KGKP Sbjct: 62 VCKQADEEFQILANFWRYSSAFTNRIFFAMVDFDEGSDVFQMLNMNSAPTFINFPPKGKP 121

Query: 149 KRGDTYELQVRGFSAEQIARWIADRTDVNIRVIRPPNYAGPLMLGLLLAVIGGLVYLRRS 208

KR DTYELQVRGFSAEQIARWIADRTDVNIRVIRPPNYAGPLMLGLLLAVIGGLVYLRRS Sbjct: 122 KRADTYELQVRGFSAEQIARWIADRTDVNIRVIRPPNYAGPLMLGLLLAVIGGLVYLRRS 181

Query: 209 NMEFLFNKTGWAFAALCFVLAMTSGQMWNHIRGPPYAHKNPHTGHVNYIHGSSQAQFVAE 268 NMEFLFNKTGWAFAALCFVLAMTSGQMWNHIRGPPYAHKNPHTGHVNYIHGSSQAQFVAE Sbjct: 182 NMEFLFNKTGWAFAALCFVLAMTSGQMWNHIRGPPYAHKNPHTGHVNYIHGSSQAQFVAE 241

Query: 269 THIVLLFNGGVTLGMVLLCEAATSDMDIGKRKIMCVAGIGLVVLFFSWMLSIFRSKYHGY 328

THIVLLFNGGVTLGMVLLCEAA ΞDMDIGKR++MC+AGIGLVVLFFSWMLSIFRSKYHGY Sb ct: 242 THIVLLFNGGVTLGMVLLCEAAASDMDIGKRRMMCIAGIGLVVLFFSWMLSIFRSKYHGY 301

Query: 329 PYSFLMS 335

PYSFLMS Sbjct: 302 PYSFLMS 308

Pedant information for DKFZphfbr2_2kl4, frame 3

Report for DKFZphfbr2_2kl4.3

[LENGTH 335

[MW] 38036.83

[pi] 9.68

[HOMOL] TREMBL:RNAF8554_1 gene: "IAG2"; product: "implantation-associated protein";

Rattus norvegicus implantation-associated protein (IAG2) mRNA, partial eds. le-161

[FUNCAT] 30.07 organization of endoplasmatic reticulum [S. cerevisiae, YOR085w]

4e-14

[FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YOR085w] 4e-14

[FUNCAT] 01.05.01 carbohydrate utilization [S. cerevisiae, YOR085w] 4e-14

[EC] 2.4.1.119 Dolιchyl-dιphosphoolιgosacchaπde--proteιn glycosyltransferase le-12

[PIRKW] glycosyltransferase le-12

[PIRKW] transmembrane protein 6e-69

[PIRKW] hexosyltransferase le-12

[PROSITE] RGD 1

[PROSITE] MYRISTYL 4

[PROSITE] AMIDATION 1

[PROSITE] CK2_PH0SPH0_SITE 2

[PROSITE] PKC_PHOSPHO_SITE 4

[PROSITE] ASN_GLYCOSYLATION 2

[KW] SIGNAL_PEPTIDE 30

[KW] TRANSMEMBRANE 4

[KW] LOW COMPLEXITY 5.97 %

SEQ MAARWRFWCVSVTMVVALLIVCDVPSASAQRKKEMVLSEKVSQLMEWTNKRPVIRMNGDK SEG PRD cccceeeeeeehhhhhhhhhhhccccccchhhhhhhhhhhhhhhhhhhccceeeeecccc MEM

SEQ FRRLVKAPPRNYSVIVMFTALQLHRQCVVCKQADEEFQILANSWRYSSAFTNRIFFAMVD SEG PRD ceeeeeccccccceeeehhhhhhccceeeehhhhhhhhhhhhhcccccccccceeeeeec MEM

SEQ FDEGSDVFQMLNMNSAPTFINFPAKGKPKRGDTYELQVRGFSAEQIARWIADRTDVNIRV SEG PRD cccccceeeecccccccceeeccccccccccceeeeeeeccchhhhhhhhhhhhheeeee

MEM M

SEQ IRPPNYAGPLMLGLLLAVIGGLVYLRRSNMEFLFNKTGWAFAALCFVLAMTSGQMWNHIR

SEG xxxxxxxxxxxxxxxxxxxx

PRD eccccccchhhhhhhhhhhcchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccceeec

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMM ...

SEQ GPPYAHKNPHTGHVNYIHGSSQAQFVAETHIVLLFNGGVTLGMVLLCEAATSDMDIGKRK

SEG

PRD ccccccccccccceeeecccchhhhhhhheeeeeeccchhhhhhhhhhhhcccccccccc

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ IMCVAGIGLVVLFFSWMLSIFRSKYHGYPYSFLMS SEG PRD eeeecccceeeeeehhhhhhhhhhccccccccccc MEM MMMMMMMMMMMMMMMMMMMMMMMMMM

Prosite for DKFZphfbr2 2kl4.3

PS00001 71->75 ASN_GLYCOSYLATION PDOC00001 PS00001 215->219 ASN_GLYCOΞYLATION PDOC00001 PS00005 38->41 PKC_PHOSPHO_SITE PDOC00005 PS00005 48->51 PKC PHOSPHO SITE PDOC00005 PS00005 103-->106 PKC PHOSPHO SITE PDOC00005

PS00005 111- ->114 PKC PHOSPHO ^"SITE PDOC00005

PS00006 208- ->212 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 292- ->296 CK2 PHOSPHO- ^"SITE PDOC00006

PS00008 193- ->199 MYRISTYL PDOC00008

PS00008 233- ->239 MYRISTYL PDOC00008

PS00008 259- ->265 MYRISTYL PDOC00008

PS00008 278- ->284 MYRISTYL PDOC00008

PS00009 296- ->300 AMIDATION PDOC00009

PS00016 150- ->153 RGD PDOC00016

(No Pfam data available for DKFZphfbr2_2kl4.3)

DKFZphfbr2_3cl8

group: nucleic acid management

DKFZphfbr2_3cl8 encodes a novel 448 ammo acid protein with strong similarity to mus musculus RNA helicase and several RNA-dependent ATPases from the DEAD box family.

RNA helicases comprise a large family of proteins that are involved in basic biological systems such as nuclear and mitochondrial splicing processes, RNA editing, rRNA processing, translation initiation, nuclear mRNA export, and mRNA degradation. RNA helicases are essential factors in cell development and differentiation, and some of them play a role m transcription and replication of viral single-stranded RNA genomes. The members of the largest subgroup, the DEAD and DEAH box proteins, exhibit a strong dependence of the unwinding activity on ATP hydrolysis. The novel protein contains a DEAD-box and is a new member of this subgroup.

The new protein can find application in modulating RNA metabolism and gene expression. strong similarity to RNA helicase and RNA-dependent ATPase from the DEAD box family group helicases

Summary DKFZphfbr2_3cl8 encodes a novel 448 ammo acid protein with similarity to DEAD-box subfamily ATP-dependent RNA helicases.

Deletion of the yeast homolouge DBP5 is lethal. strong similarity to RNA helicase and RNA-dependent ATPase from the DEAD box family complete cDNA, EST hits complete eds ATG at Bp 109

Sequenced by AGOWA

Locus: /map="87.50 cR from top of Chrl6 linkage group"

Insert length: 1713 bp

Poly A stretch at pos. 1696, no polyadenylation signal found

1 TGGGGTAGTG GGGCTGGAGC AGAGCCTGCC GCGAACCCCC GGAGCCCACG

51 ATCCCTCGTG CCATCCCTCG AATCCACCAG CACGAGCGTC CCACCCGCGC

101 CTGGGACCAT GGCCACTGAC TCATGGGCCC TGGCGGTGGA CGAGCAGGAA

151 GCTGCGGCTG AGTCGTTGAG CAACTTGCAT CTTAAGGAAG AGAAAATCAA

201 ACCAGATACC AATGGTGCTG TTGTCAAGAC CAATGCCAAT GCAGAGAAGA

251 CAGATGAAGA AGAGAAAGAG GACAGAGCTG CCCAGTCCTT ACTCAACAAG

301 CTGATCAGAA GCAACCTTGT TGATAACACA AACCAAGTGG AAGTCCTGCA

351 GCGGGATCCA AACTCCCCTC TGTACTCGGT GAAGTCTTTT GAAGAGCTTC

401 GGCTCCCACA GAACTTAATT GCCCAATCTC AGTCTGGTAC TGGTAAAACA

451 GCTGCCTTCG TGCTGGCCAT GCTTAGCCAA GTAGAACCTG CAAACAAATA

501 CCCCCAGTGT CTATGTCTCT CCCCAACGTA TGAGCTCGCC CTCCAAACAG

551 GAAAAGTGAT TGAACAAATG GGCAAATTTT ACCCTGAACT GAAGCTAGCT

601 TATGCTGTTC GAGGCAATAA ATTGGAAAGA GGCCAGAAGA TCAGTGAGCA

651 GATTGTCATT GGCACCCCTG GGACTGTGCT GGACTGGTGC TCCAAGCTCA

701 AGTTCATTGA TCCCAAGAAA ATCAAGGTGT TTGTTCTGGA TGAGGCTGAT

751 GTCATGATAG CCACTCAGGG CCACCAAGAT CAGAGCATCC GCATCCAGAG

801 GATGCTGCCC AGGAACTGCC AGATGCTGCT TTTCTCCGCC ACCTTTGAAG

851 ACTCTGTGTG GAAGTTTGCC CAGAAAGTGG TCCCAGACCC AAACGTTATC

901 AAACTGAAGC GTGAGGAAGA GACCCTGGAC ACCATCAAGC AGTACTATGT

951 CCTGTGCAGC AGCAGAGACG AGAAGTTCCA GGCCTTGTGT AACCTCTACG

1001 GGGCCATCAC CATTGCTCAA GCCATGATCT TCTGCCATAC TCGCAAAACA

1051 GCTAGTTGGC TGGCAGCAGA GCTCTCAAAA GAAGGCCACC AGGTGGCTCT

1101 GCTGAGTGGG GAGATGATGG TGGAACAGAG GGCTGCAGTG ATTGAGCGCT

1151 TCCGAGAGGG CAAAGAGAAG GTTTTGGTGA CCACCAACGT GTGTGCCCGC

1201 GGCATTGATG TTGAACAAGT GTCTGTCGTC ATCAACTTTG ATCTTCCCGT

1251 GGACAAGGAC GGGAATCCTG ACAATGAGAC CTACCTGCAC CGGATCGGGC

1301 GCACGGGCCG CTTTGGCAAG AGGGGCCTGG CAGTGAACAT GGTGGACAGC

1351 AAGCACAGCA TGAACATCCT GAACAGAATC CAGGAGCATT TTAATAAGAA

1401 GATAGAAAGA TTGGACACAG ATGATTTGGA CGAGATTGAG AAAATAGCCA

1451 ACTGAGAAGC TCCACCAGCC ACTGATGCCA GCCCTGGCAC TGCCCCTGCA

1501 CAGGAGACAA GTGCGTTCAG GGCACAGGCC CCGACATCAC CCCAAGGACA

1551 ACGGCACAAG TAGAGAGAAA CTACCTACCT CACTTCAAAT TATGTTTGGA

1601 CTTGACAAAA ATGTATGCAA ATGATGGGGG ATGGTAGAAA AAAATTATTT

1651 ACACAACCTT GGAAGATTAG GCATGAATAC ACAGAGATTT ACCTTTAAAA

1701 AAAAAAAAAA AAA

BLAST Results Entry G36496 from database EMBL:

SHGC-53094 Human Homo sapiens STS cDNA.

Length = 459

Minus Strand HSPs:

Score = 1693 (254.0 bits), Expect = 2.8e-70, P = 2.8e-70

Identities = 369/387 (95%), Positives = 369/387 (95%)

Entry G44014 from database EMBLNEW:

WIAF-3643-STS Human THudson SANGER Homo sapiens STS genomic, sequence tagged site. Score = 901, P = 2.3e-35, identities = 183/185

Medline entries

94192995:

Gene 1994 Mar 25; 140 (2) : 171-177

Mouse erythroid cells express multiple putative RNA helicase genes exhibiting high sequence conservation from yeast to mammals.

Peptide information for frame 1

ORF from 109 bp to 1452 bp; peptide length: 446 Category: strong similarity to known protein

1 MATDSWALAV DEQEAAAESL SNLHLKEEKI KPDTNGAVVK TNANAEKTDE

51 EEKEDRAAQS LLNKLIRSNL VDNTNQVEVL QRDPNSPLYS VKSFEELRLP

101 QNLIAQSQSG TGKTAAFVLA MLSQVEPANK YPQCLCLSPT YELALQTGKV

151 IEQMGKFYPE LKLAYAVRGN KLERGQKISE QIVIGTPGTV LDWCSKLKFI

201 DPKKIKVFVL DEADVMIATQ GHQDQSIRIQ RMLPRNCQML LFSATFEDSV

251 WKFAQKVVPD PNVIKLKREE ETLDTIKQYY VLCSSRDEKF QALCNLYGAI

301 TIAQAMIFCH TRKTASWLAA ELSKEGHQVA LLSGEMMVEQ RAAVIERFRE

351 GKEKVLVTTN VCARGIDVEQ VSVVINFDLP VDKDGNPDNE TYLHRIGRTG

401 RFGKRGLAVN MVDSKHSMNI LNRIQEHFNK KIERLDTDDL DEIEKIAN

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_3cl8, frame 1

PIR: 149731 RNA helicase - mouse, N = 2, Score = 1758, P = 3.8e-223

TREMBL :AF005239_1 gene: "Dbp80"; product: "DEAD-box helicase"; Drosophila melanogaster DEAD-box helicase (DbpδO) mRNA, complete eds., N = 2, Score = 1142, P = 1.8e-125

SWISSPROT:YB66_SCHPO PUTATIVE ATP-DEPENDENT RNA HELICASE C12C2.06., N = 2, Score = 911, P = 5.5e-103

PIR:S66920 probable RNA helicase CA5/6 - yeast (Saccharomyces cerevisiae), N = 2, Score = 887, P = 1.9e-98

>PIR:I49731 RNA helicase - mouse Length = 478

HSPs:

Score = 1758 (263.8 bits), Expect = 3.8e-223, Sum P(2) = 3.8e-223 Identities = 338/349 (96%), Positives = 349/349 (100%)

Query: 100 PQNLIAQSQSGTGKTAAFVLAMLSQVEPANKYPQCLCLSPTYELALQTGKVIEQMGKFYP 159

PQNLIAQSQSGTGKTAAFVLAMLS+VEPA++YPQCLCLSPTYELALQTGKVIEQMGKF+P Sbjct: 130 PQNLIAQSQSGTGKTAAFVLAMLSRVEPADRYPQCLCLSPTYELALQTGKVIEQMGKFHP 189

Query: 160 ELKLAYAVRGNKLERGQKISEQIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIAT 219

ELKLAYAVRGNKLERGQK+SEQIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIAT Sbjct: 190 ELKLAYAVRGNKLERGQKVSEQIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIAT 249

Query: 220 QGHQDQSIRIQRMLPRNCQMLLFSATFEDSVWKFAQKVVPDPNVIKLKREEETLDTIKQY 279 QGHQDQSIRIQR++PRNCQMLLFSATFEDSVWKFAQKVVPDPN+IKLKREEETLDTIKQY

Sbjct: 250 QGHQDQSIRIQRIVPRNCQMLLFSATFEDSVWKFAQKVVPDPNIIKLKREEETLDTIKQY 309

Query: 280 YVLCSSRDEKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVALLΞGEMMVE 339 YVLC++R+EKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVALLSGEMMVE

Sbjct: 310 YVLCNNREEKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVALLΞGEMMVE 369

Query: 340 QRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRT 399 QRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRT

Sbjct: 370 QRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRT 429

Query: 400 GRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN 448 GRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN

Sbjct: 430 GRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN 478

Score ^: = 419 (62.9 bits), Expect = 3.8e-223, Sum P(2) = 3.8e-223

Identities ^:= 94/136 (69%), Positives = 104/136 (76%)

Query: 1 MATDSWALAVDEQEAAAESLSNLHLKEEKIKPDTNGAVVKTNANAEKTDEEEKEDRAAQS 60 MATDSWALAVDEQEAA +S+S+L +KEEK K DTNG V+KT+ AEKT+EEEKEDRAAQS

Sbjct: 1 MATDSWALAVDEQEAAVKSMSSLQIKEEKAKSDTNG-VIKTSTTAEKTEEEEKEDRAAQΞ 59

Query: 61 LLNKLIRSNLVDNTNQVEVLQRDPNSPLYSVKSFEELRL-PQNL IAQSQSGTGKTAA 116

LLNKLIRSNLVDNTNQVEVLQRDP+SPLYSVKSFEELRL PQ L A + K

Sbjct: 60 LLNKLIRSNLVDNTNQVEVLQRDPSSPLYSVKSFEELRLKPQLLQGVYAMGFNRPSKIQE 119

Query: 117 FVLAMLSQVEPANKYPQ 133

L M+ P N Q

Sbjct: 120 NALPMMLAEPPQNLIAQ 136

Pedant information for DKFZphfbr2_3cl8, frame 1

Report for DKFZphfbr2_3cl8.1

[LENGTH] 448

[MW] 50490.07

[pi] 5.83

[HOMOL] PIR:I49731 RNA helicase - mouse 0.0

[FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YOR046c] le-102

[FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YDR021w] 2e-65

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YDR021w] 2e-65

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YJL138c] le-63

[FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae,

YJL138C] le-63

[FUNCAT] 04.99 other transcription activities [S. cerevisiae, YDL160c] 2e-49

[FUNCAT] j mrna translation and ribosome biogenesis [H. influenzae, HI0231 RNA] 9e-4£

[FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YDL084w] le-43

[FUNCAT] 1 genome replication, transcription recombination and repair [H . in luenzae HI0892] 3e-39

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YLL008w] le-35

[FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YJL033w] 9e-27

[FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YMR290c] 8e-26

[FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YDR194C] le-23

[FUNCAT] r general function prediction [M. jannaschn, MJ1401] 9e-08

[FUNCAT] 11.10 cell death [S. cerevisiae, YMR190C] le-05

[FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YMR190c] le-05

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YIR002c] 7e-04

[BLOCKS] BL00039D DEAD-box subfamily ATP-dependent helicases proteins

[BLOCKS] BL00039C DEAD-box subfamily ATP-dependent helicases proteins

[BLOCKS] BL00039B DEAD-box subfamily ATP-dependent helicases proteins

[BLOCKS] BL00039A DEAD-box subfamily ATP-dependent helicases proteins

[PIRKW] nucleus 4e-64

[PIRKW] RNA binding le-64

[PIRKW] DEAD box 4e-64

[PIRKW] transmembrane protein 3e-22

[PIRKW] DNA binding 2e-32

[PIRKW] ATP le-101

[PIRKW] purine nucleotide binding 4e-64

[PIRKW] P-loop le-101

[PIRKW] hydrolase 4e-43

[PIRKW] protein biosynthesis le-64

[PIRKW] ATP binding 2e-35

[SUPFAM] WW repeat homology 3e-29

[SUPFAM] translation initiation factor eIF-4A le-64

[SUPFAM] DEAD/H box helicase homology le-101

[SUPFAM] DNA helicase recG 2e-06

[SUPFAM] unassigned DEAD/H box helicases le-101

[SUPFAM] ATP-dependent RNA helicase DBP1 9e-33 [SUPFAM] ATP-dependent RNA helicase DHH1 4e-48

[SUPFAM] tobacco ATP-dependent RNA helicase DB10 3e-29

[PROSITE] MYRISTYL 5

[PROSITE] AMIDATION 1

[PROSITE] CK2_PHOSPHO_SITE 6

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE 8

[PROSITE] ASN_GLYCOSYLATION 1

[PFAM] Helicases conserved C-terminal domain

[PFAM] DEAD and DEAH box helicases

[KW] Alpha_Beta

SEQ MATDSWALAVDEQEAAAESLSNLHLKEEKIKPDTNGAVVKTNANAEKTDEEEKEDRAAQS PRD ccchhhhhhhhhhhhhhhhcccchhhhhhhcccccceeeeeehhhhhhhhhhhhhhhhhh

SEQ LLNKLIRSNLVDNTNQVEVLQRDPNSPLYSVKSFEELRLPQNLIAQSQSGTGKTAAFVLA PRD hhhhhhhhhcccccceeeeeeccccccceeehhhhhhhhccceeeeeccccccchhhhhh

SEQ MLSQVEPANKYPQCLCLSPTYELALQTGKVIEQMGKFYPELKLAYAVRGNKLERGQKISE

PRD hhhhhhhhhccceeeeeccchhhhhhhhhhhhhhccccccccceeeccccchhhhhhhhe

SEQ QIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIATQGHQDQSIRIQRMLPRNCQML PRD eeeecccccchhhhhhhhhhcccceeeeeecchhhhhhhccchhhhhhhhhhccccceee

SEQ LFSATFEDSVWKFAQKVVPDPNVIKLKREEETLDTIKQYYVLCSSRDEKFQALCNLYGAI PRD eeeccccchhhhhhhhhhcccceeeehhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhch

SEQ TIAQAMIFCHTRKTASWLAAELSKEGHQVALLSGEMMVEQRAAVIERFREGKEKVLVTTN PRD hhhhhheeecchhhhhhhhhhhhhccceeeeecccchhhhhhhhhhhhccccceeeeeec

SEQ VCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRTGRFGKRGLAVNMVDSKHSMNI PRD ccccccceeeeeeeeecccccccccccccceeeeeecccccccccceeeeeeeccchhhh

SEQ LNRIQEHFNKKIERLDTDDLDEIEKIAN PRD hhhhhhhhhhhccccccccchhhhhccc

Prosite for DKFZphfbr2_3cl8.1

PS00001 389->393 ASN_GLYCOSYLATION PDOC00001 PS00002 109->113 GLYCOSAMINOGLYCAN PDOC00002 PS00005 90->93 PKC_PHOSPHO_SITE PDOC00005 PS00005 111->114 PKC_PHOSPHO_SITE PDOC00005 PS00005 147->150 PKC_PHOSPHO_SITE PDOC00005 PS00005 226->229 PKC_PHOSPHO_SITE PDOC00005 PS00005 275->278 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 284->287 PKC_PHOSPHO_SITE PDOC00005 PS00005 311->314 PKC_PHOSPHO_SITE PDOC00005 PS00005 399->402 PKC_PHOSPHO_SITE PDOC00005 PS00006 48->52 CK2_PHOSPHO_SITE PDOC00006 PS00006 93->97 CK2_PHOSPHO_SITE PDOC00006 PS00006 123->127 CK2_PHOSPHO_SITE PDOC00006 PS00006 189->193 CK2_PHOSPHO_SITE PDOC00006 PS00006 245->249 CK2_PHOSPHO_SITE PDOC00006 PS00006 284->288 CK2_PHOSPHO_SITE PDOC00006 PS00008 110->116 MYRISTYL PDOC00008 PS00008 175->181 MYRISTYL PDOC00008 PS00008 185->191 MYRISTYL PDOC00008 PS00008 385->391 MYRISTYL PDOC00008 PS00008 406->412 MYRISTYL PDOC00008 PS00009 402->406 AMIDATION PDOC00009

Pfam for DKFZphfbr2_3cl8.1

HMM_NAME DEAD and DEAH box helicases

HMM *gLpPWILRnIyeMGFEkPTPIQQqAIPιILeG.... RDVMACAQTGSGK

++ ++ +N ++ P E+ +++A++Q+G+GK

Query 65 LIRSNLVDNTNQVEVLQRDPNSPLYΞVKSFEELRLPQNLIAQSQSGTGK 113

HMM TAAFlIPMLQHIDwdPWpqpPQdPrALILAPTRELAMQIQEEcRkFgkHM TAAF++ ML+++ + + PQ +L L+PT ELA+Q+ ++++++GK++ Query 114 TAAFVLAMLSQVEPAN—KYPQ CLCLSPTYELALQTGKVIEQMGKFY 158

HMM nglRImcIYGGtnMRdQMRmLeRGpPHIVIATPGRLIDHIER.gtldLDr

+ ++ + ++ ++ +++ +++ +IVI+TPG ++D + +D ++ Query 159 PELKLAYAVR GNKLERGQKISEQIVIGTPGTVLDWCSKLKFIDPKK 204

HMM IeMLVMDEADRMLD.MGFIDQIRrlMrqlPMpwNRQTMMFSATMPdelqE I+++V+DEAD M+ +G +DQ Rl R++P +N Q ++FSAT+ D++ +

Query 205 IKVFVLDEADVMIATQGHQDQSIRIQRMLP—RNCQMLLFSATFEDSVWK 252

HMM LARrFMRNPIRInldMdElTtnEnlkQwYiyVerEMWKfdcLcrLIe* +A ++ +P I ++++E T++ +IKQ+Y+ + + ++KF +LC+L++

Query 253 FAQKVVPDPNVIKLKREEETLD-TIKQYYVLCSSRDEKFQALCNLYG 298

HMM_NAME Helicases conserved C-terminal domain

HMM *EιleeWLknlGIrvmYIHGdMpQeERdeIMddFNnGEynVLIcTDVggR

+L+ +L+++G +V+ + G M+ E+R ++++F++G+ +VL++T+V +R

Query 316 SWLAAELSKEGHQVALLSGEMMVEQRAAVIERFREGKEKVLVTTNVCAR 364

HMM GIDIPdVNHVINYDM....PWNPEq.. YIQRIGRTgRIG*

GID+++V++VIN+D+ + NP++ Y++RIGRTGR+G Query 365 GIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRTGRFG 403

Medline

PMID: 10322435

"Unwinding RNA in DEAD-box proteins and related families." de la Cruz J, Kressler D, Linder

P

DKFZphfbr2 3fl6

group: brain derived

DKFZphfbr2_3f16 encodes a novel 127 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . unknown complete cDNA, complete eds, EST hits

Sequenced by AGOWA

Locus : unknown

Insert length: 1514 bp

Poly A stretch at pos. 1454, polyadenylation signal at pos. 1434

1 GGGGGGACTG GAGAAGGGAG GCGGCGGGCG AAGCGCACGT CGAGCGGGGG

51 AGCGGCGCTG CCTGTGGAGA TCCGCGGAGG CCGACAGGAT TCGTTGGCTG

101 CCGTCCCCGC TGCTGTGCAT TGGGTTAAAA ACGACAACCA ACATCAGCCA

151 TGAAAGATCC AAGTCGCAGC AGTACTAGCC CAAGCATCAT CAATGAAGAT

201 GTGATTATTA ACGGTCATTC TCATGAAGAT GACAATCCAT TTGCAGAGTA

251 CATGTGGATG GAAAATGAAG AAGAATTCAA CAGACAAATA GAAGAGGAGT

301 TATGGGAAGA AGAATTTATT GAACGCTGTT TCCAAGAAAT GCTGGAAGAG

351 GAAGAAGAGC ATGAATGGTT TATTCCAGCT CGAGATCTCC CACAAACTAT

401 GGACCAAATC CAAGACCAGT TTAATGACCT TGTTATCAGT GAAGGCTCTT

451 CTCTGGAAGA TCTTGTGGTC AAGAGCAATC TGAATCCAAA TGCAAAGGAG

501 TTTGTTCCTG GGGTGAAGTA CGGAAATATT TGAGTAGACG GGGCCCTCTT

551 TTGGTGGATG TAGCACAATT TCCACACTGT GAAGGCAGTA TTAGAAGACT

601 TAATTGTAAA AGCACTCTTG TCACTGTGTT ACACTTATGC ATTGCCAAAG

651 TTTTTGTTAG TCTTGCATGC TTAATAAAAG TGCTGAGACT GTTACTAAGT

701 AAAAAGCTGT CAAACATTTA CTGAAAATAG AATTGGCCCC ATGGCTTGAT

751 GTGAAGACAG CAAGGAAAGA AGCACCAGTC AAGTTGTGAA CAAGCACCAA

801 ATTAAAAGAC CTAAACCTTA CCAAATTGTC TTTTTTTGAG GCTAATCTAT

851 CACTTGTTAA TGTCTAAACT TTAAAATCAG TACATTTAAT TTGAGTTCCA

901 ACTGTTAAGC ATATTTCTCA GACTTAAATT TGATTATGTC CCCATCAAAA

951 AGAATCTCCA TTTTCTGAAG GTCTGTTAGT TAATTTGAGA TAATTTGTTA

1001 AAGGCAAGTA TGTCATATTA CTGAGGCTAC AAGTTAGTCA GCAGATGAGT

1051 GCCAGTCCAG CCTTTTCCGG TATGTTATTG TTAGAAATAT TGAGTTCTAA

1101 TGTTACATCT GAGGAAGTAT GTAATTTGAG AATTGTAACT TCTAAGGGAT

1151 TCACTGCATC ATAGCTATGC CTGTATGGAG TCTAACATAT GACCAATACC

1201 AACCCATAAT CCAGCTGAAC AAAGATACTG TAACATTATG ATTTGAGTGG

1251 TGCTTTTCCT TGCTTTGTTA ACCATCACGA GAGTCTGCAG CACAACTTTT

1301 AACAAAGCTA GAACAGTTTT GGCTTCTTAA ACTTCATATT TGGGTAGGTT

1351 AAGCTGCCAT ACGTGTTCAG TGTGAATAGT GTTTAAGTTG AAAATATTGT

1401 AAAAAAATTA TATTTTTTCA AAAATATTTA AAAAAATAAA TAATAGTAGA

1451 ACTGAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAGAAAAA

1501 AAAAAAAAAA AAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 150 bp to 530 bp; peptide length: 127 Category: putative protein

1 MKDPSRSSTS PSIINEDVII NGHSHEDDNP FAEYMWMENE EEFNRQIEEE 51 LWEEEFIERC FQEMLEEEEE HEWFIPARDL PQTMDQIQDQ FNDLVISEGS 101 SLEDLVVKSN LNPNAKEFVP GVKYGNI

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_3f16, frame 3

No Alert BLASTP hits found

Pedant information for DKFZphfbr2_3f16, frame 3

Report for DKFZphfbr2_3f16.3

[LENGTH] 127

[MW] 14998.41

[pi] 4.04

[BLOCKS] BL01269D

[PROSITE] MYRISTYL 1

[PROSITE] CK2_PHOSPHO_SITE

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 27.56 %

SEQ MKDPSRSSTSPSIINEDVIINGHSHEDDNPFAEYMWMENEEEFNRQIEEELWEEEFIERC

SEG xxxxxxxxxxxxxxxxxxxxxxx

PRD ccccccccccccccccceeeecccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ FQEMLEEEEEHEWFIPARDLPQTMDQIQDQFNDLVISEGSSLEDLVVKSNLNPNAKEFVP

SEG xxxxxxxxxxxx

PRD' hhhhhhhhhhhhhccccccccchhhhhhhhhcceeeecccccceeeeecccccccccccc

SEQ GVKYGNI SEG PRD ccccccc

Prosite for DKFZphfbr2_3f16.3

PS00006 24->28 CK2_PHOSPHO_SITE PDOC00006 PS00006 100->104 CK2_PHOSPHO_SITE PDOC00006 PS00008 121->127 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2 3fl6.3)

DKFZphfbr2_3g8

group: metabolism

DKFZphfbr2_3g8.1 encodes a novel 178 amino acid protein with similarity to yeast ARDl protein.

In yeast, ARDl and NAT1, are required for the expression of an N-termmal protein acetyltransferase 1. NAT1 controls full repression of the silent mating type locus HML, sporulation and entry into GO. ARDl is involved in the assembly of the NAT 1-complex. The new protein could be part of this or an other NAT complex.

The new protein can find application modulating NAT assembly and action and therefore be important in metabolism of drugs and environmental mutagens. strong similarity to N-TERMINAL ACETYLTRANSFERASE COMPLEX ARDl homolog complete cDNA, complete eds? start at Bp 40, EST hits

Sequenced by AGOWA

Locus: /map="20"

Insert length: 1030 bp

Poly A stretch at pos. 1013, no polyadenylation signal found

1 TGGGCTTGGC GAACGGTCTT CGGAAGCGGC GGCGGCGCGA TGACCACGCT 51 ACGGGCCTTT ACCTGCGACG ACCTGTTCCG CTTCAACAAC ATTAACTTGG 101 ATCCACTTAC AGAAACTTAT GGGATTCCTT TCTACCTACA ATACCTCGCC 151 CACTGGCCAG AGTATTTCAT TGTTGCAGTG GCACCTGGTG GAGAATTAAT 201 GGGTTATATT ATGGGTAAAG CAGAAGGCTC AGTAGCTAGG GAAGAATGGC 251 ACGGGCACGT CACAGCTCTG TCTGTTGCCC CAGAATTTCG ACGCCTTGGT 301 TTGGCTGCTA AACTTATGGA GTTACTAGAG GAGATTTCAG AAAGAAAGGG 351 TGGGTTTTTT GTGGATCTCT TTGTAAGAGT ATCTAACCAA GTTGCAGTTA 401 ACATGTACAA GCAGTTGGGC TACAGTGTAT ATAGGACGGT CATAGAGTAC 451 TATTCGGCCA GCAACGGGGA GCCTGATGAG GACGCTTATG ATATGAGGAA 501 AGCACTTTCC AGGGATACTG AGAAGAAATC CATCATACCA TTACCTCATC 551 CTGTGAGGCC TGAAGACATT GAATAACCCT GGGCAGTGGT TCTTAGGCAG 601 ATACTCTAGA TGCTTTATGG ACAATATTAT TTTCATTGGA TGATTCTGGA 651 GCTCTATTAG GAGAAAAGTA ATCATTTTAG GTCTTAAAGA CTTCAAGAAA 701 ATACAGGTTA TCAATTTATT TTAAATCTCA TTGTTTCCAG TTAGCAATAT 751 CATACCTATT AAAGCTGTTC ATTGTAACAA AATTCAATCA AAAAGGCAGC 801 TAGGTCAGAA GGAAACATAC CACTCTCATG GTTCATAGTA TTCACTGTAT 851 GTATGCTAGG GAAAAGACTT GCTCCAGTCT CCTCCTCAGT TCTGTGCCTG 901 AGAACCACTG CTGCATATAT TTGTTTTTAA ATTTTGTATT GAACTGTTAA 951 TTGAAGCTTT AAAAGCATAT ATGAAATGTA TAAATCTAAG ATGTATAATA 1001 CATTATTGAC TCCAAAAAAA AAAAAAAAAA

BLAST Results

Entry HSG0101 from database EMBL: human STS SHGC-35956.

Length = 401

Minus Strand HSPs:

Score = 1417 (212.6 bits), Expect = 9.3e-58, P = 9.3e-5S

Identities = 301/311 (96%)

Medline entries

No Medline entry

Peptide information for frame 1

ORF from 40 bp to 573 bp; peptide length: 178 Category: strong similarity to known protein

1 MTTLRAFTCD DLFRFNNINL DPLTETYGIP FYLQYLAHWP EYFIVAVAPG 51 GELMGYIMGK AEGSVAREEW HGHVTALSVA PEFRRLGLAA KLMELLEEIS 101 ERKGGFFVDL FVRVSNQVAV NMYKQLGYSV YRTVIEYYSA SNGEPDEDAY 151 DMRKALSRDT EKKSIIPLPH PVRPEDIE

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_3g8, frame 1

TREMBL :SPCC16C4_12 gene: "SPCC16C4.12"; product: "putative n-terminal acetyltransferase complex subunit"; S. pombe chromosome III cosmid C16C4., N = 1, Score = 475, P = 3.2e-45

SWISSPROT:ARDH_LEIDO N-TERMINAL ACETYLTRANSFERASE COMPLEX ARDl SUBUNIT HOMOLOG., N = 1, Score = 451, P = 1. le-42

PIR:S69021 hypothetical protein YPR131C - yeast (Saccharomyces cerevisiae), N = 1, Score = 382, P = 2.3e-35

>TREMBL:SPCC16C4_12 gene: "SPCC16C4.12"; product: "putative n-terminal acetyltransferase complex subunit"; S. pombe chromosome III cosmid cl6C4. Length = 180

HSPs:

Score = 475 (71.3 bits), Expect = 3.2e-45, P = 3.2e-45 Identities = 96/165 (58%), Positives = 118/165 (71%)

Query: 1 MTTLRAFTCDDLFRFNNINLDPLTETYGIPFYLQYLAHWPEYFIVAVAPGGE—LMGYIM 58

MT R F DLF FNNINLDPLTET+ I FYL YL WP +V + + LMGYIM Sbjct: 1 MTDTRKFKATDLFSFNNINLDPLTETFNISFYLSYLNKWPSLCVVQESDLSDPTLMGYIM 60

Query: 59 GKAEGSVAREEWHGHVTALSVAPEFRRLGLAAKLMELLEEISERKGGFFVDLFVRVSNQV 118

GK+EG+ +EWH HVTA++VAP RRLGLA +M+ LE + + FFVDLFVR SN + Sbjct: 61 GKSEGT—GKEWHTHVTAITVAPNSRRLGLARTMMDYLETVGNSENAFFVDLFVRASNAL 118

Query: 119 AVNMYKQLGYSVYRTVIEYYSASNGEPDEDAYDMRKALSRDTEKKSI 165

A++ YK LGYSVYR VI YYS +G+ DED++DMRK LSRD ++SI Sbjct: 119 AIDFYKGLGYSVYRRVIGYYSNPHGK-DEDSFDMRKPLSRDVNRESI 164

Pedant information for DKFZphfbr2_3g8, frame 1

Report for DKFZphfbr2_3g8.1

[LENGTH] 178 [MW] 20338.24 [pi] 5.06 [HOMOL] TREMBL:SPCC16C4_12 gene: "SPCC16C4.12"; product: "putative n-terminal acetyltransferase complex subunit"; S. pombe chromosome III cosmid cl6C4. 7e-47 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YPR131c] 6e-37 [ FUNCAT ] 01.06.07 lipid, fatty-acid and sterol utilization [S. cerevisiae, YHR013c] 4e-14 [FUNCAT] 30.03 organization of cytoplasm [S cerevisiae, YHR013c] 4e-14 [FUNCAT] 03.22 cell cycle control and mitosis [S cerevisiae, YHR013c] 4e-14 [FUNCAT] r general function prediction [M jannaschn, MJ1530] 6e-09 [PIRKW] acyltransferase le-12 [SUPFAM] arrest-defective protein 1 le-12 [SUPFAM] Escherichia coli peptide N-acetyltransferase riml le-07 [PROSITE] CK2_PHOSPHO_SITE 3 [PROSITE] PKC_PHOSPHO_SITE 3 [KW] Alpha_Beta

SEQ MTTLRAFTCDDLFRFNNINLDPLTETYGIPFYLQYLAHWPEYFIVAVAPGGELMGYIMGK PRD ccccccccccchhhhhhcccccccccccchhhhhhcccccceeeeeeccccceeeehhhh

SEQ AEGSVAREEWHGHVTALSVAPEFRRLGLAAKLMELLEEISERKGGFFVDLFVRVSNQVAV PRD hcccccccccccceeeeehhhhhhhhcchhhhhhhhhhhhhhccceeeeeeeecchhhhh

SEQ NMYKQLGYSVYRTVIEYYSASNGEPDEDAYDMRKALSRDTEKKSIIPLPHPVRPEDIE PRD hhhhhhcccchhhhhhccccccccccchhhhhhhhhhhhhhhhhcccccccccccccc

Prosite for DKFZphfbr2_3g8.1 PS00005 3->6 PKC_PHOSPHO_SITE PDOC00005

PS00005 100->103 PKC_PHOSPHO_SITE PDOC00005

PS00005 160->163 PKC_PHOSPHO_SITE PDOC00005

PS00006 8->12 CK2_PHOSPHO_SITE PDOC00006

PS00006 133->137 CK2_PHOSPHO_SITE PDOC00006

PS00006 141->145 CK2 PHOSPHO SITE PDOC00006

(No Pfam data available for DKFZphfbr2_3g8.1)

DKFZphfbr2_312

group: brain derived

DKFZphfbr2_312 encodes a novel 589 amino acid protein with weak similarity to S. cerevisiae ubiquitin-like protein DSK2.

Pfam predicts for this protein similarity to the ubiquitin family; No informative BLAST results; No predictive prosite or SCOP motive

The new protein can find application in studying the expression profile of brain-specific genes . similarity to ubiquitm-like protein DSK2 yeast complete cDNA, complete eds, EST hits

Dsk2p is involved m spindel pole body SPB duplication, SPB = centomer strong similarity to HRIHFB2157 human mRNA

Sequenced by AGOWA

Locus: unknown

Insert length: 2978 bp

Poly A stretch at pos. 2958, polyadenylation signal at pos. 2924

1 GGGGGGAGGA AGCGGTGGCT GCTGCGGATG TCGGTGTGAG CGAGCGGCGC

51 CTGAACACAC GGCGGCTGCC GAGCGCCTGA CCCGGGCCTG CGCCAGAGCC

101 TGCACCGAGC TCCGGGGCCC CACACCCGCT ACGGTGGCCC TGCGCCCGTT

151 GCTACTGAGG CGGCGTGCTC TGCATTCTTC GCTGTCCAGG CCTGCCGGCT

201 CTGGTGTCTG CTGGCTCCTC CTTGCTCGCC TGCTCCCTCC TGCTTGCCTG

251 AGTCACCGCC GCCGCCGCCG CCACAGCCAT GGCCGAGAGT GGTGAAAGCG

301 GCGGTCCTCC GGGCTCCCAG GATAGCGCCG CCGGAGCCGA AGGTGCTGGC

351 GCCCCCGCGG CCGCTGCCTC CGCGGAGCCC AAAATCATGA AAGTCACCGT

401 GAAGACCCCG AAGGAAAAGG AGGAATTCGC CGTGCCCGAG AATAGCTCCG

451 TCCAGCAGTT TAAGGAAGAA ATCTCTAAAC GTTTTAAATC ACATACTGAC

501 CAACTTGTGT TGATATTTGC TGGAAAAATT TTGAAAGATC AAGATACCTT

551 GAGTCAGCAT GGAATTCATG ATGGACTTAC TGTTCACCTT GTCATTAAAA

601 CACAAAACAG GCCTCAGGAT CATTCAGCTC AGCAAACAAA TACAGCTGGA

651 GGCAATGTTA CTACATCATC AACTCCTAAT AGTAACTCTA CATCTGGTTC

701 TGCTACTAGC AACCCTTTTG GTTTAGGTGG CCTTGGGGGA CTTGCAGGTC

751 TGAGTAGCTT GGGTTTGAAT ACTACCAACT TCTCTGAACT ACAGAGTCAG

801 ATGCAGCGAC AACTTTTGTC TAACCCTGAA ATGATGGTCC AGATCATGGA

851 AAATCCCTTT GTTCAGAGCA TGCTCTCAAA TCCTGACCTG ATGAGACAGT

901 TAATTATGGC CAATCCACAA ATGCAGCAGT TGATACAGAG AAATCCAGAA

951 ATTAGTCATA TGTTGAATAA TCCAGATATA ATGAGACAAA CGTTGGAACT

1001 TGCCAGGAAT CCAGCAATGA TGCAGGAGAT GATGAGGAAC CAGGACCGAG

1051 CTTTGAGCAA CCTAGAAAGC ATCCCAGGGG GATATAATGC TTTAAGGCGC

1101 ATGTACACAG ATATTCAGGA ACCAATGCTG AGTGCTGCAC AAGAGCAGTT

1151 TGGTGGTAAT CCATTTGCTT CCTTGGTGAG CAATACATCC TCTGGTGAAG

1201 GTAGTCAACC TTCCCGTACA GAAAATAGAG ATCCACTACC CAATCCATGG

1251 GCTCCACAGA CTTCCCAGAG TTCATCAGCT TCCAGCGGCA CTGCCAGCAC

1301 TGTGGGTGGC ACTACTGGTA GTACTGCCAG TGGCACTTCT GGGCAGAGTA

1351 CTACTGCGCC AAATTTGGTG CCTGGAGTAG GAGCTAGTAT GTTCAACACA

1401 CCAGGAATGC AGAGCTTGTT GCAACAAATA ACTGAAAACC CACAACTGAT

1451 GCAAAACATG TTGTCTGCCC CCTACATGAG AAGCATGATG CAGTCACTAA

1501 GCCAGAATCC TGACCTTGCT GCACAGATGA TGCTGAATAA TCCCCTATTT

1551 GCTGGAAATC CTCAGCTTCA AGAACAAATG AGACAACAGC TCCCAACTTT

1601 CCTCCAACAA ATGCAGAATC CTGATACACT ATCAGCAATG TCAAACCCTA

1651 GAGCAATGCA GGCCTTGTTA CAGATTCAGC AGGGTTTACA GACATTAGCA

1701 ACGGAAGCCC CGGGCCTCAT CCCAGGGTTT ACTCCTGGCT TGGGGGCATT

1751 AGGAAGCACT GGAGGCTCTT CGGGAACTAA TGGATCTAAC GCCACACCTA

1801 GTGAAAACAC AAGTCCCACA GCAGGAACCA CTGAACCTGG ACATCAGCAG

1851 TTTATTCAGC AGATGCTGCA GGCTCTTGCT GGAGTAAATC CTCAGCTACA

1901 GAATCCAGAA GTCAGATTTC AGCAACAACT GGAACAACTC AGTGCAATGG

1951 GATTTTTGAA CCGTGAAGCA AACTTGCAAG CTCTAATAGC AACAGGAGGT

2001 GATATCAATG CAGCTATTGA AAGGTTACTG GGCTCCCAGC CATCATAGCA

2051 GCATTTCTGT ATCTTGAAAA AATGTAATTT ATTTTTGATA ACGGCTCTTA

2101 AACTTTAAAA TACCTGCTTT ATTTCATTTT GACTCTTGGA ATTCTGTGCT

2151 GTTATAAACA AACCCAATAT GATGCATTTT AAGGTGGAGT ACAGTAAGAT

2201 GTGTGGGTTT TTCTGTATTT TTCTTTTCTG GAACAGTGGG AATTAAGGCT

2251 ACTGCATGCA TCACTTCTGC ATTTATTGTA ATTTTTTAAA AACATCACCT

2301 TTTATAGTTG GGTGACCAGA TTTTGTCCTG CATCTGTCCA GTTTATTTGC

2351 TTTTTAAACA TTAGCCTATG GTAGTAATTT ATGTAGAATA AAAGCATTAA

2401 AAAGAAGCAA ATCATTTGCA CTCTATAATT TGTGGTACAG TATTGCTTAT

2451 TGTGACTTTG GCATGCATTT TTGCAAACAA TGCTGTAAGA TTTATACTAC

2501 TGATAATTTT GTTTTATTTG TATACAATAT AGAGTATGCA CATTTGGGAC 2551 TGCATTTCTG GAAACATACT GCAATAGGCT CTCTGAGCAA AACACCTGTA 2601 ACTAAAAAAG TGAAGATAAG AAAATACTCT TAAAGCTGAG TATTTCCTAA 2651 TTGTATAGAA TCTTACAGCA TCTTTGACAA ACATCTCCCA GCAAAAGTGC 2701 CGGTTAGTCA GGTTTGTTGA AAATACAGTA GAAAAGCTGA TTCTGGTTAT 2751 CTCTTTAAGG ACAATTAATT GTACAGACAC ATAATGTAAC ATTGTCTCAA 2801 CATTCATTCA CAGATTGACT GTAAATTACC TTAATCTTTG TGCAGACTGA 2851 AGGAACACTG TAGTATACCC CAAAGTGCAT TTGCCTAGGA CTTCTCAGCT 2901 TCTCCCATAG GTAGTTTAAC AGGCATTAAA ATTTGTAATT GAAATGTTGC 2951 TTTCACTCAA AAAAAAAAAA AAAAAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 279 bp to 2045 bp; peptide length: 589 Category: similarity to known protein

1 MAESGESGGP PGSQDSAAGA EGAGAPAAAA SAEPKIMKVT VKTPKEKEEF

51 AVPENSSVQQ FKEEISKRFK SHTDQLVLIF AGKILKDQDT LSQHGIHDGL

101 TVHLVIKTQN RPQDHSAQQT NTAGGNVTTS STPNSNSTSG SATSNPFGLG

151 GLGGLAGLSS LGLNTTNFSE LQSQMQRQLL SNPEMMVQIM ENPFVQSMLS

201 NPDLMRQLIM ANPQMQQLIQ RNPEISHMLN NPDIMRQTLE LARNPAMMQE

251 MMRNQDRALS NLESIPGGYN ALRRMYTDIQ EPMLSAAQEQ FGGNPFASLV

301 SNTSSGEGΞQ PSRTENRDPL PNPWAPQTSQ SSSASSGTAS TVGGTTGSTA

351 SGTSGQSTTA PNLVPGVGAS MFNTPGMQSL LQQITENPQL MQNMLSAPYM

401 RSMMQSLSQN PDLAAQMMLN NPLFAGNPQL QEQMRQQLPT FLQQMQNPDT

451 LSAMSNPRAM QALLQIQQGL QTLATEAPGL IPGFTPGLGA LGSTGGSSGT

501 NGSNATPSEN TSPTAGTTEP GHQQFIQQML QALAGVNPQL QNPEVRFQQQ

551 LEQLSAMGFL NREANLQALI ATGGDINAAI ERLLGSQPS

BLASTP hits

Entry CE1_1 from database TREMBL:

"F15C11.2"; Caenorhabditis elegans cosmid VF15C11L

Length = 293

Score = 454 (159.8 bits), Expect = 4.4e-43, P = 4.4e-43

Identities = 81/162 (50%), Positives = 113/162 (69%)

Entry S54583 from database PIR: ubiquitin-like protein DSK2 - yeast (Saccharomyces cerevisiae)

Length = 373

Score = 278 (97.9 bits), Expect = 1.2e-23, P = 1.2e-23

Identities = 100/307 (32%), Positives = 155/307 (50%)

Entry AB015344_1 from database TREMBLNEW: gene: "HRIHFB2157"; Homo sapiens HRIHFB2157 mRNA, partial eds.

Score = 1135, P = 3.6e-115, identities = 227/301, positives = 253/301

Alert BLASTP hits for DKFZphfbr2_312, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_312, frame 3

Report for DKFZphfbr2 312.3

[LENGTH] 589

[MW] 62489.22

[pi] 5.02

[HOMOL] TREMBL :AB015344_1 gene: "HRIHFB2157"; Homo sapiens HRIHFB2157 mRNA, partial cds. le-121

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YMR276w] 2e-17 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YMR276w] 2e-17

[BLOCKS] BL00299 Ubiquitin family proteins

[SUPFAM] unassigned ubiquitin-related proteins 5e-16

[SUPFAM] ubiquitin homology 5e-16

[PROSITE] MYRISTYL 24

[PROSITE] CK2_PHOSPHO_SITE

[PROSITE] GLYCOSAMINOGLYCAN

[PROSITE] PKC_PHOSPHO_SITE

[PROSITE] ASN_GLYCOSYLATION

[PFAM] Ubiquitin family

[KW] Irregular

[KW] 3D

[KW] LOW COMPLEXITY 23.43 %

SEQ MAESGESGGPPGSQDSAAGAEGAGAPAAAAΞAEPKIMKVTVKTPKEKEEFAVPENSΞVQQ

SEG ..xxxxxxxxxxx..xxxxxxxxxxxxxxxxxxx...xxxxxxxxxxxx laarA CEEEEEETTTCEEEECTTTTBHHH

SEQ FKEEISKRFKΞHTDQLVLIFAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQDHSAQQT SEG laarA HHHHHHHHHCCCGGGEEEEETTEECTTTTBGGGGCCTTTTEEEEEBC

SEQ NTAGGNVTTSSTPNSNSTSGSATSNPFGLGGLGGLAGLSSLGLNTTNFSELQSQMQRQLL SEG ... xxxxxxxxxxxxxxxxxxxxxx ..xxxxxxxxxxxxxxxx laarA

SEQ SNPEMMVQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHMLNNPDIMRQTLE SEG laarA

SEQ LARNPAMMQEMMRNQDRALSNLESIPGGYNALRRMYTDIQEPMLSAAQEQFGGNPFASLV SEG laarA

SEQ SNTSSGEGSQPSRTENRDPLPNPWAPQTSQSSSASSGTASTVGGTTGSTASGTSGQSTTA SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx laarA

SEQ PNLVPGVGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMMQSLSQNPDLAAQMMLN SEG laarA

SEQ NPLFAGNPQLQEQMRQQLPTFLQQMQNPDTLSAMSNPRAMQALLQIQQGLQTLATEAPGL SEG laarA

SEQ IPGFTPGLGALGSTGGSSGTNGSNATPSENTSPTAGTTEPGHQQFIQQMLQALAGVNPQL SEG .... xxxxxxxxxxxxxxxxxxxxxxxx laarA

SEQ QNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPS SEG laarA

Prosite for DKFZphfbr2_312.3

PS00001 55->59 ASN_GLYCOSYLATION PDOC00001 PS00001 126->130 ASN_GLYCOSYLATION PDOC00001 PS00001 136->140 ASN_GLYCOSYLATION PDOC00001 PS00001 164->168 ASN_GLYCOSYLATION PDOC00001 PS00001 167->171 ASN_GLYCOSYLATION PDOC00001 PS00001 302->306 ASN_GLYCOSYLATION PDOC00001 PS00001 501->505 ASN_GLYCOSYLATION PDOC00001 PS00002 305->309 GLYCOSAMINOGLYCAN PDOC00002 PS00005 40->43 PKC_PHOSPHO_SITE PDOC00005 PS00005 43->46 PKC_PHOSPHO_SITE PDOC00005 PS00005 66->69 PKC_PHOSPHO_SITE PDOC00005 PS00006 43->47 CK2_PHOSPHO_SITE PDOC00006 PS00006 71->75 CK2_PHOSPHO_SITE PDOC00006 PS00006 181->185 CK2_PHOSPHO_SITE PDOC00006 PS00006 200->204 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 260->264 CK2_PHOSPHO_SITE PDOC00006 PS00006 304->308 CK2_PHOSPHO_SITE PDOC00006 PS00006 312->316 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 506->510 CK2_PHOΞPHO_SITE PDOC00006 PS00006 572->576 CK2_PHOSPHO_SITE PDOC00006 PS00008 8->14 MYRISTYL PDOC00008 PS00008 12->18 MYRISTYL PDOC00008 SO

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DKFZphfbr2_62bll

group: signal transduction

DKFZphfbr2_62bll .encodes a novel 655 amino ac d putative GTPase-activating protein, related to human chimaerins .

The rac small GTPase is associated with type-I phosphatidylinositol 4-phosphate 5-kιnase and regulating the production of phosphatidylinositol 4, 5-bιsphosphate . The new protein is expected to activate p21rac-related small GTPases.

The new protein can find clinical application in modulating/blocking the response to a cellular receptor.

similarity to CHIMAERIN complete cDNA, complete eds, EST hits

Sequenced by LMU

Locus: /map="4"

Insert length: 4593 bp

Poly A stretch at pos. 4571, polyadenylation signal at pos. 4553

1 GGGGGAGTTT GAAGACAGAA AGGAAAGGGG AGAAACCTGC AGAGAGCATC

51 AAAGGATGGG GGGTGCTATA AAAGAAGCAG GGGGGTCCTT TGAAAGAAAT

101 CTATCATGCA CTGAAATGCT TTCTGGAGAA GGTGCCGTTA TTTTCCTCCC

151 CTCTTGCTCA GATGAAAGGA GCCAGCAAGG ACAGTCCTGA AATATTCCTC

201 AGGGGACTTT TTGTCATTGT TCCTCTTTCC TCTTGCACAG AGCTATTTGC

251 TGACCTTTCC AGAGGAATCT CAGTCCAGCT GAGAAGACAG TTCTTAATAA

301 AAACAAAAAA ATGCAAAAAC CAATTCCTGC TGTTTGAATG GGAATGGTAG

351 CTTGCTTGCT GCAGTTCTTT TCCTGTGACA TTTTGGAATG TCTGCAGAAA

401 CTTAAAAAAA AGAAAAAAAA AACCTTAAAA ACTCCCTGGA TTAGGCAAGA

451 GAAAAGGAAG TTTTTTTTTG CTAAACAGGA GTAAATGAGA GGTGGTAACT

501 TATCCCTAAG CCAGGACCTG GATGATCAAA ACCTTCAAAT TCTAGGGATC

551 AGCACTTCAA AAATAACAAG TAAACAAGCA TGAGGAGTGG CTGTTGGGTT

601 TCGCTCAGAG GCAGGTTTTA AAGGAAGCCA AAACCGGGTT CAGAACTTCA

651 GGCCTGTACG ATGCCTGAAG ACCGGAATTC TGGGGGGTGC CCGGCTGGTG

701 CCTTAGCCTC AACTCCTTTC ATCCCTAAAA CTACATACAG AAGAATCAAA

751 CGGTGTTTTA GTTTTCGGAA AGGCATTTTT GGACAGAAAC TGGAGGATAC

801 TGTTCGTTAT GAGAAGAGAT ATGGGAACCG TCTGGCTCCG ATGTTGGTGG

851 AGCAGTGCGT GGACTTTATC CGACAAAGGG GGCTGAAAGA AGAGGGTCTC

901 TTTCGACTGC CAGGCCAGGC TAATCTTGTT AAGGAGCTCC AAGATGCCTT

951 TGACTGTGGG GAGAAGCCAT CATTTGACAG CAACACAGAT GTACACACGG

1001 TGGCATCACT TCTTAAGCTG TACCTCCGAG AACTTCCAGA ACCAGTTATT

1051 CCTTATGCGA AGTATGAAGA TTTTTTGTCA TGTGCCAAAC TGCTCAGCAA

1101 GGAAGAGGAA GCAGGTGTTA AGGAATTAGC AAAGCAGGTG AAGAGTTTGC

1151 CAGTGGTAAA TTACAACCTC CTCAAGTATA TTTGCAGATT CTTGGATGAA

1201 GTACAGTCCT ACTCGGGAGT TAACAAAATG AGTGTGCAGA ACTTGGCAAC

1251 GGTCTTTGGT CCTAATATCC TGCGCCCCAA AGTGGAAGAT CCTTTGACTA

1301 TCATGGAGGG CACTGTGGTG GTCCAGCAGT TGATGTCAGT GATGATTAGC

1351 AAACATGATT GCCTCTTTCC CAAAGATGCA GAACTACAAA GCAAGCCCCA

1401 AGATGGAGTG AGCAACAACA ATGAAATTCA GAAGAAAGCC ACCATGGGGC

1451 TGTTACAGAA CAAGGAGAAC AATAACACCA AGGACAGCCC TAGTAGGCAG

1501 TGCTCCTGGG ACAAGTCTGA GTCACCCCAG AGAAGCAGCA TGAACAATGG

1551 ATCCCCCACA GCTCTATCAG GCAGCAAAAC CAACAGCCCA AAGAACAGTG

1601 TTCACAAGCT AGATGTGTCT AGAAGCCCCC CTCTCATGGT CAAAAAGAAC

1651 CCAGCCTTTA ATAAGGGTAG TGGGATAGTT ACCAATGGGT CCTTCAGCAG

1701 CAGTAATGCA GAAGGTCTTG AGAAAACCCA AACCACCCCC AATGGGAGCC

1751 TACAGGCCAG AAGGAGCTCT TCACTGAAGG TATCTGGTAC CAAAATGGGC

1801 ACGCACAGTG TACAGAATGG AACGGTGCGC ATGGGCATTT TGAACAGCGA

1851 CACACTCGGG AACCCCACAA ATGTTCGAAA CATGAGCTGG CTGCCAAATG

1901 GCTATGTGAC CCTGAGGGAT AACAAGCAGA AAGAACAAGC TGGAGAGTTA

1951 GGCCAGCACA ACAGACTGTC CACCTATGAT AATGTCCATC AACAGTTCTC

2001 CATGATGAAC CTTGATGACA AGCAGAGCAT TGACAGTGCT ACCTGGTCCA

2051 CTTCCTCCTG TGAAATCTCC CTCCCTGAGA ACTCCAACTC CTGTCGCTCT

2101 TCTACCACCA CCTGCCCAGA GCAAGACTTT TTTGGGGGGA ACTTTGAGGA

2151 CCCTGTTTTG GATGGGCCCC CGCAGGACGA CCTTTCCCAC CCCAGGGACT

2201 ATGAAAGCAA AAGTGACCAC AGGAGTGTGG GAGGTCGAAG TAGTCGTGCC

2251 ACCAGTAGCA GTGACAACAG TGAGACATTT GTGGGCAACA GCAGCAGCAA

2301 CCACAGTGCA CTGCACAGTT TAGTTTCCAG CCTGAAACAG GAAATGACCA

2351 AACAGAAGAT AGAGTATGAG TCCAGGATAA AGAGCTTAGA ACAGCGAAAC

2401 TTGACTTTGG AAACAGAAAT GATGAGCCTC CATGATGAAC TGGATCAGGA

2451 GAGGAAAAAG TTCACAATGA TAGAAATAAA AATGCGAAAT GCCGAGCGAG

2501 CAAAAGAAGA TGCCGAGAAA AGAAATGACA TGCTACAGAA AGAAATGGAG

2551 CAGTTTTTTT CCACGTTTGG AGAACTGACA GTGGAACCCA GGAGAACCGA 2601 GAGAGGAAAC ACAATATGGA TTCAGTGAGC CTGCTTTCGC CTGCTGTCTC 2651 TGATGGCTCT GGCAAGGACT CCAGGGATTC TGGTGGGATA TGACTTAGAA 2701 CCAGGTGGCT GGTCACCTGG ATGTACAGAA GTCTAACTGG TGAAGGAATA 2751 TCATTTACAG ACATTAAACA TCCATATCTG CAATGTGTAC CAAAGTTATA 2801 TCATGCCCCA TAATGCTACT GTCAAGTGTT ACAACTGGAT ATGTGTATAT 2851 AGAGTAGTTT TTCAAAAGTA AACTAAAAAT GAGAAGCATA TTTCAAGAAT 2901 TATTTTATTG CAAGTCTTGT ATTTAAATGT TAAATCAATA TGTTGTTGCA 2951 ATTTAGCTTG CTTTCAAGCT TCACCCCTTG CACTTAACAT AAGCTATTTT 3001 TGGCATTGTG TTATCATCGG CTTATTTTAT AGATCAATAT TTTTATTTCC 3051 CTTTTTTGCT GAGGAAATGA AGATAAGCAA AAATATAAAT ATATATATAA 3101 ATATATGAGT TATTAAAACC AGAAGAATAC TTTGTGGCTG TGCTGTTTGT 3151 GCCAATAGAC TTTGTCATGA CCAAAAAGAG AAATGTAAAT AGTTTTATAA 3201 AATACAGTCG AATCACCAGG AACCTTTGAG CTGCTTTTAA AATTCTTCCC ₃251 CTGGCACCAC TCAGTTTTGC TTTTGCGAGG CGATTTGACA TAGGAACTTT 3301 GAGACTCCAT GAGAAAGTCC CTTTCTGAGG CCCACTGTCT ACCTTGCCAG 3351 ATCCTCAGTG CGTATCGCCA ATGCAGGATG CTCCTTAGAA AAGAAAAAAT 3401 GGTAAAGGAT GGCATTTAAC GATTCAGGCT TTGAATTACT CTGTCCCTCT 3451 GGACCGAATC TCTTTAACTG CTGGATAGTT TTAGAGGAAT TCTCCTGCTA 3501 CTTAGGTACT GGGAAACAAT GCTTGCTAAA CCATGCCCAC GTGAGCACCT 3551 GTCTCCCACT CAAACCTCTC CCATCTCCCA ACAACTGCAC TTTAGAATAC 3601 CAGCAGTGAA ATGGTATTAC TGTTTCCCTC TGAGTGAAAC TGCTAGAGTA 3651 TATGTCACGT AGTGACATTT TTTTCTCACT CAGGCTATTG CCATCTGGGA 3701 TTCTCTCCCT ACTACAGCTG GCAAAGTTGG TTTGCAGCAA GAAGATAGTG 3751 GGAGGGGGCC AGGCTGCAGG AGAAGGAGAA AAGTTTAGAA GAAACAAACC 3801 ATTTTGCTTC TAATTTTGAC AGTATCACTT TCCTGTTAAA ACATACAATA 3851 ATTTTAAAAG GTGAATGCCT AAAGTTCCAA TTTTAGCAAA TATGGGAACC 3901 TCAGCAATGC TAATTTTCTA GAAAAACCCA GGGCTCTTTG GAGCTAGAGT 3951 TTTGGGAGAA CAGTTCTTCA CAATAAGGCA ATGGTTTTGA GAGGCCAGGC 4001 AAATAATCTT TCTCACCGTA GAACAAAAAG TTACAAAAGG CATAATCGGA 4051 AATAGAGACT ACATACTTGA GTTTATGGGG TTTGTGTTGT TTGAAGGTTC 4101 AATGCTTGCA TGTGTTTATT TATTTTCAAG AGGGAAAGTG GTCTGTACTG 4151 CTTTCATCCT TGCCACTGTC TTGCTTTTAT TTTTTACTCT CCCACTGAGC 4201 AAGCGTCTGT GGTCCTATGG TATCAACCAG TATCTTTATA GCAATAATTT 4251 CTTTAATTCC CTTTTCTCTC TCTTTCCAAT TATTTAACCA GTTACTTCCA 4301 CCTGGACATA CGATAGGAAA TTCAAACTCA AAATATGAAA ATTGATCTTA 4351 ATAACTCTCC CTTCATATCT TTTCACCTAT TTCCAGTCCT TATCATAGTT 4401 GATAAAAACC TCAGACTCAT CCAGAAAGCT ATATGATGCA CTAGTAAAAA 4451 AAACAAAGAT ATTTAAACTG CTTGGGTTCA AATGGTATAC AATTTGCCAG 4501 CTGTTACTGA ACCTTCTATG CATAACTTTT TTTTTCCTCT GTGCAATTGG 4551 AATAATAAAA ATACTACTCC CATAAAAAAA AAAAAAAAAA AAC

BLAST Results

Entry G38474 from database EMBLNEW:

SHGC-58303 Human Homo sapiens STS genomic, sequence tagged site.

Score = 2175, P = 1.2e-92, identities = 439/441

Medline entries

97476250:

Beta2-ch maeπn is a high affinity receptor for the phorbol ester tumor promoters .

Peptide information for frame 1

ORF from 661 bp to 2625 bp; peptide length: 655 Category: similarity to known protein

1 MPEDRNSGGC PAGALASTPF IPKTTYRRIK RCFSFRKGIF GQKLEDTVRY

51 EKRYGNRLAP MLVEQCVDFI RQRGLKEEGL FRLPGQANLV KELQDAFDCG

101 EKPSFDSNTD VHTVASLLKL YLRELPEPVI PYAKYEDFLS CAKLLSKEEE

151 AGVKELAKQV KSLPVVNYNL LKYICRFLDE VQSYSGVNKM SVQNLATVFG

201 PNILRPKVED PLTIMEGTVV VQQLMSVMIS KHDCLFPKDA ELQSKPQDGV

251 SNNNEIQKKA TMGLLQNKEN NNTKDSPSRQ CSWDKSESPQ RSSMNNGSPT

301 ALSGSKTNSP KNSVHKLDVS RSPPLMVKKN PAFNKGSGIV TNGSFSSSNA

351 EGLEKTQTTP NGSLQARRSS SLKVSGTKMG THSVQNGTVR MGILNSDTLG

401 NPTNVRNMSW LPNGYVTLRD NKQKEQAGEL GQHNRLSTYD NVHQQFSMMN

451 LDDKQSIDSA TWSTSSCEIS LPENSNSCRS STTTCPEQDF FGGNFEDPVL

501 DGPPQDDLSH PRDYESKSDH RSVGGRSSRA TSSSDNSETF VGNSSSNHSA

551 LHSLVSSLKQ EMTKQKIEYE ΞRIKSLEQRN LTLETEMMSL HDELDQERKK 601 FTMIEIKMRN AERAKEDAEK RNDMLQKEME QFFSTFGELT VEPRRTERGN 651 TIWIQ

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_62bll, frame 1

SWISSPROT:Y053_HUMAN HYPOTHETICAL PROTEIN KIAA0053., N = 3, Score = 661, P = 2.4e-89

TREMBL :HSU90908_1 product: "unknown"; Human clones 23549 and 23762 mRNA, complete eds., N = 1, Score = 348, P = l.le-29

PIR:S29128 N-chimerin - rat, N = 1, Score = 286, P = 2.8e-24

PIR:S29956 beta-chimerin - rat, N = 1, Score = 279, P = 1.6e-23

TREMBL:AB014572_1 gene: "KIAA0672"; product: "KIAA0672 protein"; Homo sapiens mRNA for KIAA0672 protein, complete eds., N = 1, Score = 314, P = le-24

>SWISSPROT:Y053_HUMAN HYPOTHETICAL PROTEIN KIAA0053. Length = 638

HSPs:

Score = 661 (99.2 bits), Expect = 2.4e-89, Sum P(3) = 2.4e-89 Identities = 122/209 (58%), Positives = 160/209 (76%)

Query: 38 GIFGQKLEDTVRYEKRYGNRLAPMLVEQCVDFIRQRGLKEEGLFRLPGQANLVKELQDAF 97

G+FGQ+L++TV YE+++G L P+LVE+C +FI + G EEG+FRLPGQ NLVK+L+DAF Sbjct: 148 GVFGQRLDETVAYEQKFGPHLVPILVEKCAEFILEHGRNEEGIFRLPGQDNLVKQLRDAF 207

Query: 98 DCGEKPSFDSNTDVHTVASLLKLYLRELPEPVIPYAKYEDFLSCAKLLSKEEEAGVKELA 157

D GE+PSFD +TDVHTVAΞLLKLYLR+LPEPV+P+++YE FL C +L + +E +EL Sbjct: 208 DAGERPSFDRDTDVHTVASLLKLYLRDLPEPVVPWSQYEGFLLCGQLTNADEAKAQQELM 267

Query: 158 KQVKSLPVVNYNLLKYICRFLDEVQSYSGVNKMSVQNLATVFGPNILRPKVEDPLTIMEG 217

KQ+ LP NY+LL YICRFL E+Q VNKMSV NLATV G N++R KVEDP IM G Sbjct: 268 KQLSILPRDNYΞLLSYICRFLHEIQLNCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRG 327

Query: 218 TVVVQQLMSVMISKHDCLFPKDAELQSKP 246

T +Q++M++MI H+ LFPK ++ P Sbjct: 328 TPQIQRVMTMMIRDHEVLFPKSKDIPLSP 356

Score = 210 (31.5 bits), Expect = 2.4e-89, Sum P(3) = 2.4e-89 Identities = 45/115 (39%), Positives = 73/115 (63%)

Query: 531 TSSSDNSETFVGNSSSNHSALHSL VΞSLKQEMTKQKIEYESRIKSLEQRNLTLETEM 587

T +S NSET G +Ξ + SL V L++E+ QK YE +IK+LE+ N + ++ Sbjct: 523 TLASPNSETGPGKKNSGEEEIDSLQRMVQELRKEIETQKQMYEEQIKNLEKENYDVWAKV 582

Query: 588 MSLHDELDQERKKFTMIEIKMRNAERAKEDAEKRNDMLQKEMEQFFSTFGELTVE 642

+ L++EL++E+KK +EI +RN ER++ED EKRN L++E+++F + E E Sbjct: 583 VRLNEELEKEKKKSAALEIΞLRNMERSREDVEKRNKALEEEVKEFVKSMKEPKTE 637

Score = 70 (10.5 bits), Expect = 1.2e-74, Sum P(3) = 1.2e-74 Identities = 28/121 (23%), Positives = 54/121 (44%)

Query: 528 ΞRATSSSDNSETFVGNSΞSNHSALHSLVSSLKQE-MTKQKIEYESRIKSLEQRNL-TLET 585

S+ TS+ DN + G+ SAL S K + + E K+ + + +L+ Sbjct: 489 SQRTSTYDNVPSLPGSPGEEASALSSQACDSKGDTLASPNSETGPGKKNSGEEEIDSLQR 548

Query: 586 EMMSLHDELDQERKKFTMIEIKMRNAERAKEDAEKRNDMLQKEMEQFFSTFGELTVEPRR 645

+ L E++ +++ M E +++N E+ D + L +E+E+ L + R Sbjct: 549 MVQELRKEIETQKQ MYEEQIKNLEKENYDVWAKVVRLNEELEKEKKKSAALEISLRN 605

Query: 646 TER 648

ER Sbjct: 606 MER 608

Score = 53 (8.0 bits), Expect = 2.4e-89, Sum P(3) = 2.4e-89 Identities = 31/111 (27%), Positives = 46/111 (41%)

Query: 344 SFSSSNAEGLEKTQTTPNGSLQARRSSSLKVSGTKMGTHSVQNG TV--RMGILNSD 397

SFSS ++ + T T A S KV K G +Q+ T+ R L S Sbjct: 388 SFSSMTSDS-DTTSPTGQQPSDAFPEDSSKVPREKPGDWKMQSRKRTQTLPNRKCFLTSA 446 Query: 398 TLG-NPTNV RNMSWLPNGYVTLRDNKQKEQAGELGQ HNRLSTYDNV 442

G N + + +N W P+ + ++ + +L Q R STYDNV Sbjct: 447 FQGANSSKMEIFKNEFWSPSSEAKAGEGHRRTMSQDLRQLSDSQRTSTYDNV 498

Score = 53 (8.0 bits), Expect = 3.5e-14, Sum P(3) = 3.5e-14 Identities = 32/125 (25%), Positives = 56/125 (44%)

Query: 242 LQSKPQDG VSNNNEIQKKATMGLLQNKEN--NNTKD SPSRQCSWDKSESPQRΞS 293

++SK +D + +IQ+ TM ++++ E +KD SP Q + K RSS Sbjct: 314 IRSKVEDPAVIMRGTPQIQRVMTM-MIRDHEVLFPKSKDIPLSPPAQKNDPKKAPVARSS 372 Query: ₂94 MNNGSPTALSGSKTNSPKNSVHKLDVSRSPPLMVKKNPAFNKGSGIVTNGSFSSSNAEGL 353

+ + L S+T+S + D + P + + AF + S V + Sbjct: 373 VGWDATEDLRISRTDSFSSMTSDSDTTS—PTGQQPSDAFPEDSSKVPREKPGDWKMQSR 430 Query: 354 EKTQTTPN 361

++TQT PN Sbjct: 431 KRTQTLPN 438

Pedant information for DKFZphfbr2_62bll, frame 1

Report for DKFZphfbr2_62bll .1

[LENGTH] 655

[MW] 73394.60

[pi] 8.13

[HOMOL] SWISSPROT:Y053_HUMAN HYPOTHETICAL PROTEIN KIAA0053. 3e-71

[FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins

[S. cerevisiae, YPL115c] le-16 [FUNCAT] 09.04 biogenesis of cytoskeleton [S. cerevisiae, YPL115C] le-16 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YPL115c] le-16 [FUNCAT] 10.02.09 regulation of g-protein activity [S. cerevisiae, YPL115c] le-16 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YER155c] 2e-16 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YER155C] 2e-16 [FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YDR379w] 4e-16 [FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YDL240w] 3e-15 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YOR134w] 2e-13 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YOR134w] 2e-13 [SCOP] dlrgp 1.83.1.1.1 p50 RhoGAP domain [human (Homo sapiens) 2e-46 [SCOP] dlpbwa_ 1.83.1.1.2 p85 alpha subunit RhoGAP domain [human (Hom 6e-37 [PIRKW] phosphotransferase 3e-13 [PIRKW] breakpoint cluster region 2e-20 [PIRKW] transmembrane protein 7e-14 [PIRKW] brain 2e-20 [PIRKW] alternative splicing 2e-20 [PIRKW] P-loop 9e-19 [PIRKW] cytoskeleton le-08 [SUPFAM] CDC24 homology 7e-21 [SUPFAM] bcr protein 7e-21 [SUPFAM] myosin motor domain homology 9e-19 [SUPFAM] pleckstπn repeat homology 2e-15 [SUPFAM] LIM metal-bindmg repeat homology 9e-15 [SUPFAM] protein kinase C zinc-binding repeat homology 5e-24 [PROSITE] MYRISTYL 16 [PROSITE] CAMP_PHOSPHO_SITE 3 [PROSITE] CK2_PHOSPHO_SITE 15 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 11 [PROSITE] ASN_GLYCOSYLATION 8 [KW] Irregular [KW] 3D [KW] LOW_COMPLEXITY 6.87 % [KW] COILED COIL 12.06 %

SEQ MPEDRNSGGCPAGALASTPFIPKTTYRRIKRCFSFRKGIFGQKLEDTVRYEKRYGNRLAP

SEG

COILS

1rgp- C

SEQ MLVEQCVDFIRQRGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDSNTDVHTVASLLKL

SEG

COILS lrgp- HHHHHHHHHHHHHHTTTTTTTTTCCCHHHHHHHHHHHHHCCCCCGGGCCCCHHHHHHHHH

SEQ YLRELPEPVIPYAKYEDFLSCAKLLSKEEEAGVKELAKQVKΞLPVVNYNLLKYICRFLDE

SEG COILS lrgp- HHHHTTTTTTTGGGHHHHHH TTTTCGGGHHHHHHHHHHHCCHHHHHHHHHHHHHHHH

SEQ VQSYSGVNKMSVQNLATVFGPNILRPKVEDPLTIMEGTVVVQQLMSVMISKHDCLFPKDA SEG COILS lrgp- HHHHHHHHCCCHHHHHHHHGGGCC

SEQ ELQSKPQDGVSNNNEIQKKATMGLLQNKENNNTKDSPSRQCSWDKSESPQRSSMNNGSPT SEG COILS lrgp-

SEQ ALSGSKTNSPKNSVHKLDVSRΞPPLMVKKNPAFNKGSGIVTNGSFSSSNAEGLEKTQTTP SEG COILS lrgp-

SEQ NGSLQARRSSSLKVSGTKMGTHSVQNGTVRMGILNSDTLGNPTNVRNMSWLPNGYVTLRD SEG COILS lrgp-

SEQ NKQKEQAGELGQHNRLSTYDNVHQQFSMMNLDDKQΞIDSATWSTSSCEISLPENSNSCRS SEG xxxxxx COILS lrgp-

SEQ STTTCPEQDFFGGNFEDPVLDGPPQDDLSHPRDYESKSDHRSVGGRSSRATSSSDNSETF SEG xxxxx xxxxxxxxxxxxxxxx ... COILS lrgp-

SEQ VGNSSSNHSALHSLVSSLKQEMTKQKIEYESRIKSLEQRNLTLETEMMSLHDELDQERKK SEG .. xxxxxxxxxxxxxxxx COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC lrgp-

SEQ FTMIEIKMRNAERAKEDAEKRNDMLQKEMEQFFSTFGELTVEPRRTERGNTIWIQ SEG COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC lrgp-

Prosite for DKFZphfbr2_62bll .1

PS00001 271->275 ASN_GLYCOSYLATION PDOC00001 PS00001 342->346 ASN_GLYCOSYLATION PDOC00001 PS00001 361->365 ASN_GLYCOSYLATION PDOC00001 PS00001 386->390 ASN_GLYCOSYLATION PDOC00001 PS00001 407->411 ASN_GLYCOSYLATION PDOC00001 PS00001 543->547 ASN_GLYCOSYLATION PDOC00001 PS00001 547->551 AΞN_GLYCOSYLATION PDOC00001 PS00001 580->584 ASN_GLYCOS LATION PDOC00001 PS00004 258->262 CAMP_PHOSPHO_SITE PDOC00004 PS00004 367->371 CAMP_PHOSPHO_SITE PDOC00004 PS00004 599->603 CAMP_PHOSPHO_SITE PDOC00004 PS00005 25->28 PKC_PHOSPHO_SITE PDOC00005 PS00005 34->37 PKC_PHOSPHO_SITE PDOC00005 PS00005 47->50 PKC_PHOSPHO_SITE PDOC00005 PS00005 309->312 PKC_PHOSPHO_SITE PDOC00005 PS00005 371->374 PKC_PHOSPHO_SITE PDOC00005 PS00005 388->391 PKC_PHOSPHO_SITE PDOC00005 PS00005 417->420 PKC_PHOSPHO_SITE PDOC00005 PS00005 477->480 PKC_PHOSPHO_SITE PDOC00005 PS00005 527->530 PKC_PHOSPHO_SITE PDOC00005 PS00005 557->560 PKC_PHOSPHO_SITE PDOC00005 PS00005 646->649 PKC_PHOSPHO_SITE PDOC00005 PS00006 107-M11 CK2_PHOSPHO_SITE PDOC00006 PS00006 146->150 CK2_PHOSPHO_SITE PDOC00006 PS00006 213->217 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 230->234 CK2_PHOSPHO_SITE PDOC00006 PS00006 348->352 CK2_PHOSPHO_SITE PDOC00006 PS00006 417->421 CK2_PHOSPHO_SITE PDOC00006 PS00006 437->441 CK2_PHOSPHO_SITE PDOC00006 PS00006 465->469 CK2_PHOSPHO_SITE PDOC00006 PS00006 470->474 CK2_PHOSPHO_SITE PDOC00006 PS00006 484->488 CK2_PHOSPHO_SITE PDOC00006 PS00006 516->520 CK2_PHOSPHO_SITE PDOC00006 PS00006 532->536 CK2 PHOSPHO SITE PDOC00006 SO

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DKFZphfbr2_62flO

group: intracellular transport and trafficking

DKFZphfbr2_62f10 encodes a novel 320 amino acid protein with strong similarity to mammalian zinc transporter proteins.

The novel proteins is a membrane protein, which should be involved in the transport of Zmc across the cell membrane.

The Zn-T-transporters are membrane proteins that facilitates sequestration of zinc m endosomal vesicles. In the brain, ZnT-3 mRNA seems to be involved in the accumulation of zmc in synaptie vesicles. Zinc (Zn) is an essential element in normal development and metabolism. Recent studies show that in Alzheimer s disease, Zn functions as a double-edged sword, affording protection against Alzheimer s amyloid beta peptide (the major component of senile plaques) at low concentrations and enhancing toxicity at high concentrations by accelerated aggregation of the amyloid beta peptide.

The new protein can find application in modulation of Zinc transport in neuronal cells, thus providing means for a modulation of Alzheimer's amyloid beta peptide plaque formation. strong similarity to zinc transporter proteins ; membrane regions : 5

Summary DKFZphfbr2_62f10 encodes a novel 320 amino acid protein with similarity to zinc transporter protein.

The new protein can find clinical application m modulating Zn2+ uptake . strong similarity to zmc transporter proteins complete cDNA, complete eds, few EST hits

Sequenced by LMU

Locus : unknown

Insert length: 5422 bp

Poly A stretch at pos. 5397, polyadenylation signal at pos. 5381

1 GTCTAACTTT GGAAATATCA CCCTCATGCT GTCTTCCCAG GATGTCTCTC

51 TCCCTAAGTA AGGGATGTTA CTTCCTGGAG GGAATGCAGT GTTGGGAATC

101 TGAAGACCCA GCTTTGAGCT GAATTTGCTT TGTGATACCT GGAGAGAAGA

151 CGTGTTTTCT TGACAACAGC ACAGTACCTA GTGAGTTCAA CAACAACGAC

201 AACAACAGCC GCAGCTCATC CTGGCCGTCA TGGAGTTTCT TGAAAGAGCG

251 TATCTTGTGA ATGATAAAGC TGCCAAGATG TATGCTTTCA CACTAGAAAG

301 AAGGAGCTGC AAATGAACAC TTCATAGCAA TGTGGAACTC CAACAGAAAC

351 CGGTGAATAA AGATCAGTGT CCCAGAGAGA GACCAGAGGA GCTGGAGTCA

401 GGAGGCATGT ACCACTGCCA CAGTGGCTCC AAGCCCACAG AAAAGGGGGC

451 GAATGAGTAC GCCTATGCCA AGTGGAAACT CTGTTCTGCT TCAGCAATAT

501 GCTTCATTTT CATGATTGCA GAGGTCGTGG GTGGGCACAT TGCTGGGAGT

551 CTTGCTGTTG TCACAGATGC TGCCCACCTC TTAATTGACC TGACCAGTTT

601 CCTGCTCAGT CTCTTCTCCC TGTGGTTGTC ATCGAAGCCT CCCTCTAAGC

651 GGCTGACATT TGGATGGCAC CGAGCAGAGA TCCTTGGTGC CCTGCTCTCC

701 ATCCTGTGCA TCTGGGTGGT GACTGGCGTG CTAGTGTACC TGGCATGTGA

751 GCGCCTGCTG TATCCTGATT ACCAGATCCA GGCGACTGTG ATGATCATCG

801 TTTCCAGCTG CGCAGTGGCG GCCAACATTG TACTAACTGT GGTTTTGCAC

851 CAGAGATGCC TTGGCCACAA TCACAAGGAA GTACAAGCCA ATGCCAGCGT

901 CAGAGCTGCT TTTGTGCATG CCCCTGGAGA TCTATTTCAG AGTATCAGTG

951 TGCTAATTAG TGCACTTATT ATCTACTTTA AGCCAGAGTA TAAAATAGCC

1001 GACCCAATCT GCACATTCAT CTTTTCCATC CTGGTCTTGG CCAGCACCAT

1051 CACTATCTTA AAGGACTTCT CCATCTTACT CATGGAAGGT GTGCCAAAGA

1101 GCCTGAATTA CAGTGGTGTG AAAGAGCTTA TTTTAGCAGT CGACGGGGTG

1151 CTGTCTGTGC ACTGCCTGCA CATCTGGTCT CTAACAATGA ATCAAGTAAT

1201 TCTCTCAGCT CATGTTGCTA CAGCAGCCAG CCGGGACAGC CAAGTGGTTC

1251 GGAGAGAAAT TGCTAAAGCC CTTAGCAAAA GCTTTACGAT GCACTCACTC

1301 ACCATTCAGA TGGAATCTCC AGTTGACCAG GACCCCGACT GCCTTTTCTG

1351 TGAAGACCCC TGTGACTAGC TCAGTCACAC CGTCAGTTTC CCAAATTTGA

1401 CAGGCCACCT TCAAACATGC TGCTATGCAA TTTCTGCATC ATAGAAAATA

1451 AGGAACCAAA GGAAGAAATT CATGTCATGG TGCAATGCAT ATTTTATCTA

1501 TTTATTTAGT TCCATTCACC ATGAAGGAAG AGGCACTGAG ATCCATCAAT

1551 CAATTGGATT ATATACTGAT CAGTAGCTGT GTTCAATTGC AGGAATGTGT

1601 ATATAGATTA TTCCTGAGTG GAGCCGAAGT AACAGCTGTT TGTAACTATC

1651 GGCAATACCA AATTCATCTC CCTTCCAATA ATGCATCTTG AGAACACATA

1701 GGTAAATTTG AACTCAGGAA AGTCTTACTA GAAATCAGTG GAAGGGACAA

1751 ATAGTCACAA AATTTTACCA AAACATTAGA AACAAAAAAT AAGGAGAGCC

1801 AAGTCAGGAA TAAAAGTGAC TCTGTATGCT AACGCCACAT TAGAACTTGG 1851 TTCTCTCACC AAGCTGTAAT GTGATTTTTT TTTCTACTCT GAATTGGAAA 1901 TATGTATGAA TATACAGAGA AGTGCTTACA ACTAATTTTT ATTTACTTGT 1951 CACATTTTGG CAATAAATCC CTCTTATTTC TAAATTCTAA CTTGTTTATT 2001 TCAAAACTTT ATATAATCAC TGTTCAAAAG GAAATATTTT CACCTACCAG 2051 AGTGCTTAAA CACTGGCACC AGCCAAAGAA TGTGGTTGTA GAGACCCAGA 2101 AGTCTTCAAG AACAGCCGAC AAAAACATTC GAGTTGACCC CACCAAGTTG 2151 TTGCCACAGA TAATTTAGAT ATTTACCTGC AAGAAGGAAT AAAGCAGATG 2201 CAACCAATTC ATTCAGTCCA CGAGCATGAT GTGAGCACTG CTTTGTGCTA 2251 GACATTGGGC TTAGCACTGA AACTATAAAG AGGAATCAGA CGCAGCAAGT 2301 GCTTCTGTGT TCTGGTAGCA ACTCAACACT ATCTGTGGAG AGTAAACTGA 2351 AGATGTGCAG GCCAACATTC TGGAAATCCT ATGTCAGTGG GTTTGGTTTG 2401 GAACCTGGAC TTCTGCATTT TTAAAAGTTA CCCAGAGATG CTTCTAAAGA 2451 TGAGCCATAG TCTAGAAGAT TGTCAACCAC AGGAGTTCAT TGAGTGGGAC 2501 AGCTAGACAC ATACATTGGC AGTTACAATA GTATCATGAA TTGCAATGAT 2551 GTAGTGGGGT ATAAAAGGAA AGCGATGGAT ATTGCCGGAT GGGCATGGCC 2601 AGTGATGTTT CACGTCATTG AGGTGACAGC TCTGCTGGAC TTTGAATTAC 2651 ATATGGAGGC TCTCCAGGAA GACGAAGAAG AGAAGGACAT TCTAGGCAAA 2701 AAGAAGACTA GGCACAAGGC ACACTTATGT TTGTCTGTTA GCTTTTAGTT 2751 GAAAAAGCAA AATACATGAT GCAAAGAAAC CTCTCCACGC TGTGATTTTT 2801 AAAACTACAT ACTTTTTGCA ACTTTATGGT TATGAGTATT GTAGAGAACA 2851 GGAGATAGGT CTTAGATGAT TTTTATGTTG TTGTCAGACT CTAGCAAGGT 2901 ACTAGAAACC TAGCAGGCAT TAATAATTGT TGAGGCAATG ACTCTGAGGC 2951 TATATCTGGG CCTTGTCATT ATTTATCATT TATATTTGTA TTTTTTTCTG 3001 AAATTTGAGG GCCAAGAAAA CATTGACTTT GACTGAGGAG GTCACATCTG 3051 TGCCATCTCT GCAAATCAAT CAGCACCACT GAAATAACTA CTTAGCATTC 3101 TGCTGAGCTT TCCCTGCTCA GTAGAGACAA ATATACTCAT CCCCCACCTC 3151 AGTGAGCTTG TTTAGGCAAC CAGGATTAGA GCTGCTCAGG TTCCCAACGT 3201 CTCCTGCCAC ATCGGGTTCT CAAAATGGAA AGAATGGTTT ATGCCAAATC 3251 ACTTTTCCTG TCTGAAGGAC CACTGAATGG TTTTGTTTTT CCATATTTTG 3301 CATAGGACGC CCTAAAGACT AGGTGACTTG GCAAACACAC AAGTGTTAGT 3351 ATAATTCTTT GCTTCTGCTT CTTTTTGAAA ATCATGTTTA GATTTGATTT 3401 TAAGTCAGAA ATTCACTGAA TGTCAGGTAA TCATTATGGA GGGAGATTTG 3451 TGTGTCAACC AAAGTAATTG TCCCATGGCC CCAGGGTATT TCTGTTGTTT 3501 CCCTGAAATT CTGCTTTTTT AGTCAGCTAG ATTGAAAACT CTGAACAGTA 3551 GATGTTTATA TGGCAAAATG CAAGACAATC TATAAGGGAG ATTTTAAGGA 3601 TTTTGAGATG AAAAAACAGA TGCTACTCAG GGGCTTTATG GACCATCCAT 3651 CAATTCTGAA GTTCTGACTC TCCCATTACC CTTTCCCTGG TGTGGTCAGA 3701 ACTCCAGGTC ACTGGAAGTT AGTGGAATCA TGTAGTTGAA TTCTTTACTT 3751 CAAGACATTG TATTCTCTCC AGCTATCAAA ACATTAATGA TCTTTTATGT 3801 CTTTTTTTTG TTATTGTTAT ACTTTAAGTT CTGGGGTACA TGTGCGGAAC 3851 ATGTAGGTTT GTTACATAGG TATACATGTG CCATGGTGGT TTGCTGCACT 3901 CATCAACCTG TCATCTACAT TCTTTTATGT CTGTCTTTCA AAGCAACACT 3951 CTGTTCTTCT GAGTAGTGAA ATCAGGTCAA CTTTACCACC AGCCTCCATT 4001 TTTAATATGC TTCACCATCA TCCAGCACCT ACTTAAGATT TATCTAGGGC 4051 TCTGTGGTGA TGTTAGGACC CATAAAAGAA ATTTATGCCT TCCATATGTT 4101 TGGTTACAGA TGGGAAATGG GAATGTTGAA GGACATGAAA GAAAGGATGT 4151 TTACACATTA AGCATCAGTT CTGAAGCTAG ATTGTCTGAG TTTGAATCTT 4201 AGCTCTTCCC TTTATTAGCT CTGTGACCTC GAGCTAGTTA CTTAAATGCT 4251 CTGATCCTCT ATTTCCTGAT CAGTGAAACC TCCCTATTCA AATGTGTGAG 4301 AGTTTAATAA ATTAGGACAC TTAAAAATGT TGGAGCAGTG CATAGCATGT 4351 AGTGTTCAGT ACATGTTAAA TGTTGTTTTT TATTATGTAC AAACATGTGT 4401 GGGCACAGAA TTTTAAATCA TCTCAACTTT TGAGAAATTT TGAGTTATCA 4451 ACACCGTTCC CACAAGACAG TGGCAAAATT ATTGGTGAGA ATTAAACAGC 4501 TGTTTCTCAG AGGAAGCAAT GGAGGCTTGC TGGGATAAAG GCATTTACTG 4551 AGAGGCTGTT ACCTAGTGAG AGTGATGAAT TAATTAAAAT AGTCGAATCC 4601 CTTTCTGACT GTCTCTGAAA GCTTCCGCTT TTATCTTTGA AGAGCAGAAT 4651 TGTCACCCCA AGGACATTTA TTAATAAAAA GAACAACTGT CCAGTGCAAT 4701 GAAGGCAAAG TCATAGGTCT CCCAAGTCTT ACCCCATTCC TGTGAAATAT 4751 CAAGTTCTTG GCTTTTCTCT GTCATGTAGC CTCAACTTTC TCCGACCGGG 4801 TGCATTTCTT TCTCTGGTTT CTAAATTGCC AGTGGCAAAT TTGGATCACT 4851 TACTTAATAT CTGTTAAATT TTGTGACCCA ACAAAGTCTT TTAGCACTGT 4901 GGTGTCAAAA AGAAAAACAC CTCCCAGGCA TATACATTTT ATAGATTCCT 4951 GGAGAATGTT GCTCTCCAGC TCCATCCCCA CCCAATGAAA TATGATCCAG 5001 AGAGTCTTGC AAAGAGACAA GCCTCATTTT CCACAATTAG CTCTAAAGTG 5051 CCTCCAGGAA ATGATTTTCT CAGCTCATCT CTCTGTATTC CCTGTTTTGG 5101 ATCACAGGGC AATCTGTTTA AATGACTAAT TACAGAAATC ATTAAAGGCA 5151 CCAAGCAAAT GTCATCTCTG AATACACACA TCCCAAGCTT TACAAATCCT 5201 GCCTGGCTTG ACAGTGATGA GGCCACTTAA CAGTCCAGCG CAGGCGGATG 5251 TTAAAAAAAA TAAAAAGGTG ACCATCTGCG GTTTAGTTTT TTAACTTTCT 5301 GATTTCACAC TTAACGTCTG TCATTCTGTT ACTGGGCACC TGTTTAAATT 5351 CTATTTTAAA ATGTTAATGA GTGTTGTTTA AAATAAAATC AGGAAAGAGA 5401 GAAAAAAAAA AAAAAAAAAA AC

BLAST Results o BLAST result

Medline entries 97121493:

ZnT-3, a putative transporter of zinc into synaptie vesicles.

96203098:

ZnT-2, a mammalian protein that confers resistance to zmc by facilitating vesicular sequestration .

Peptide information for frame 2

ORF from 407 bp to 1366 bp; peptide length: 320 Category: strong similarity to known protein

1 MYHCHSGSKP TEKGANEYAY AKWKLCSASA ICFIFMIAEV VGGHIAGSLA

51 VVTDAAHLLI DLTSFLLSLF SLWLSSKPPΞ KRLTFGWHRA EILGALLSIL

101 CIWVVTGVLV YLACERLLYP DYQIQATVMI IVSSCAVAAN IVLTVVLHQR

151 CLGHNHKEVQ ANASVRAAFV HAPGDLFQSI ΞVLISALIIY FKPEYKIADP

201 ICTFIFSILV LASTITILKD FSILLMEGVP KSLNYSGVKE LILAVDGVLS

251 VHCLHIWSLT MNQVILSAHV ATAASRDSQV VRREIAKALS KSFTMHSLTI

301 QMESPVDQDP DCLFCEDPCD

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_62f10, frame 2

PIR:S70632 zinc transporter ZnT-2 - rat, N = 1, Score = 884, P = 1.5e-88

TREMBL :MMU76007_1 gene: "ZnT-3"; product: "ZnT-3"; Mus musculus zmc transporter ZnT-3 (ZnT-3) mRNA, complete eds., N = 1, Score = 772, P = l.le-76

TREMBL:HSU76010_1 gene: "ZnT-3"; product: "ZnT-3"; Human putative zmc transporter ZnT-3 (ZnT-3) mRNA, complete eds., N = 1, Score = 74₂, P = 1.6e-73

TREMBL :MMUZNT02_1 gene: "ZnT-3"; product: "zinc transporter"; Mus musculus zinc transporter (ZnT-3) gene, complete eds., N = 1, Score = 715, P = 1.2e-70

TREMBL :CET18D3_3 gene: "T18D3.3"; Caenorhabditis elegans cosmid T18D3, N = 1, Score = 699, P = 5.9e-69

>PIR:S70632 zinc transporter ZnT-2 - rat Length = 359

HSPs:

Score = 884 (132.6 bits), Expect = 1.5e-88, P = 1.5e-88 Identities = 171/326 (52%), Positives = 230/326 (70%)

Query: 2 YHCHSGSKPTEKGANEYAYAKWKLCSASAICFIFMIAEVVGGHIAGSLAVVTDAAHLLID 61

++CH+ +E A+ KL ASAIC +FMI E++GG++A SLA++TDAAHLL D

Sbjct: 34 HYCHAQKDSGSHPNΞEKQRARRKLYVASAICLVFMIGEIIGGYLAQΞLAIMTDAAHLLTD 93

Query: 62 LTSFLLSLFSLWLΞSKPPSKRLTFGWHRAEILGALLSILCIWVVTGVLVYLACERLLYPD 121

S L+SLFSLW+SS+P +K + FGW RAEILGALLS+L IWVVTGVLVYLA +RL+ D Sbjct: 94 FASMLISLFSLWVSSRPATKTMNFGWQRAEILGALLSVLSIWVVTGVLVYLAVQRLISGD 153

Query: 122 YQIQATVMIIVSSCAVAANIVLTVVLHQRCLGHNH KEVQANASVRAAFVHAPG 174

Y+I+ M+I S CAVA NI++ + LHQ GH+H + Q N SVRAAF+H G Sbjct: 154 YEIKGDTMLITSGCAVAVNIIMGLALHQSGHGHSHGHSHEDSΞQQQQNPSVRAAFIHVVG 213

Query: 175 DLFQSISVLISALIIYFKPEYKIADPICTFIFΞILVLASTITILKDFSILLMEGVPKSLN 234

DL QS+ VL++A IIYFKPEYK DPICTF+FSILVL +T+TIL+D ++LMEG PK ++ Sbjct: 214 DLLQSVGVLVAAYIIYFKPEYKYVDPICTFLFSILVLGTTLTILRDVILVLMEGTPKGVD 273

Query: 235 YSGVKELILAVDGVLSVHCLHIWSLTMNQVILSAHVATAASRDSQVVRREIAKALSKSFT 294

++ VK L+L+VDGV ++H LHIW+LT+ Q +LS H+A A + D+Q V + L F Sbjct: 274 FTTVKNLLLSVDGVEALHSLHIWALTVAQPVLSVHIAIAQNVDAQAVLKVARDRLQGKFN 333 Query: 295 MHSLTIQMESPVDQDPDCLFCEDPCD 320

H++TIQ+ES + C C+ P + Sbjct: 334 FHTMTIQIESYSEDMKSCQECQGPSE 359

Pedant information for DKFZphfbr2_62f10, frame 2

Report for DKFZphfbr2_62f10.2

[LENGTH] 320

[MW] 35053.51

[pi] 6.48

[HOMOL] PIR:S70632 z c transporter ZnT-2 - rat 3e-84

[FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YMR243c] 2e-16

[FUNCAT] 13.01 homeostasis of metal ions [S. cerevisiae, YMR243c] 2e-16

[FUNCAT] 08.19 cellular import [S. cerevisiae, YMR243c] 2e-16 ,

[FUNCAT] 11.07 detoxificaton [S. cerevisiae, YMR243c] 2e-16

[FUNCAT] 07.04.01 metal ion transporters (cu, fe, etc.) [S. cerevisiae, YMR243c]

2e-16

[FUNCAT] 08.04 mitochondrial transport [S. cerevisiae, YOR316c] 3e-13

[FUNCAT] 30.16 mitochondrial organization [Ξ. cerevisiae, YOR316c] 3e-13

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YDR205w] 4e-07

[PIRKW] transmembrane protein 2e-30

[PIRKW] mitochondrial inner membrane 6e-12

[PIRKW] mitochondrion 6e-12

[PIRKW] membrane protein le-11

[SUPFAM] zmc transporter ZnT-2 2e-30

[SUPFAM] membrane protein czcD le-11

[PROSITE] MYRISTYL 4

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPH0_SITE 1

[PROSITE] PR0KAR_LIP0PROTEIN 1

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 4

[PROSITE] ASN_GLYCOSYLATION 2

[KW] TRANSMEMBRANE 5

[KW] LOW COMPLEXITY 8.12 %

SEQ MYHCHSGSKPTEKGANEYAYAKWKLCSASAICFIFMIAEVVGGHIAGSLAVVTDAAHLLI

SEG xxx

PRD cccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhh

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ DLTSFLLΞLFSLWLSSKPPSKRLTFGWHRAEILGALLΞILCIWVVTGVLVYLACERLLYP

SEG xxxxxxxxxxxxxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhc

MEM MMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ DYQIQATVMIIVSSCAVAANIVLTVVLHQRCLGHNHKEVQANASVRAAFVHAPGDLFQSI SEG PRD cccccccceeeehhhhhhhhhhhhhhhhhcccccccccccccchhhhhhhhhhhhhchhh MEM MMMMMMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMM ...

SEQ SVLISALIIYFKPEYKIADPICTFIFSILVLASTITILKDFSILLMEGVPKSLNYSGVKE SEG PRD hhhhhhhhhhcccceeeccchhhhhhhhhhhhhchhhhhhhheeeeeccccccchhhhhh MEM .. MMMMMMMMMMMMMMMMMMMM

SEQ LILAVDGVLSVHCLHIWΞLTMNQVILSAHVATAASRDSQVVRREIAKALSKSFTMHSLTI SEG PRD hhhhhhceeecccceeeeeccchhhhheeeeeccccchhhhhhhhhhhhhhhhcccccee MEM

SEQ QMESPVDQDPDCLFCEDPCD SEG PRD eeeccccccccccccccccc

MEM

Prosite for DKFZphfbr2_62f10.2

PS00001 162-5-166 ASN_GLYCOSYLATION PDOC00001 PS00001 234->238 ASN_GLYCOSYLATION PDOC00001 PS00004 81->85 CAMP_PHOSPHO_SITE PDOC00004 PS00005 11->14 PKC_PHOSPHO_SI E PDOC00005 PS00005 75->78 PKC PHOSPHO SITE PDOC00005 PS00005 80->83 PKC_PHOSPHO_SITE PDOC00005

PS00005 164->167 PKC_PHOSPHO_SITE PDOC00005

PS00006 304->308 CK2_PHOSPHO_SITE PDOC00006

PS00007 13->21 TYR_PHOSPHO_SITE PDOC00007

PS00008 7->13 MYRISTYL PDOC00008

PS00008 42->48 MYRISTYL PDOC00008

PS00008 94->100 MYRISTYL PDOC00008

PS00008 228->234 MYRISTYL PDOC00008

PS00013 125->136 PROKAR LIPOPROTEIN PDOC00013

(No Pfam data available for DKFZphfbr2_62f10.2)

DKFZphfbr2 62nl0

group: brain derived

DKFZphfbr2_62nlO encodes a novel 541 amino acid protein with similarity to Plasmodium vivax reticulocyte-binding protein 1.

The novel protein contains one Leucine Zipper, involved in protem-protem-mteraction. No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . similarity to reticulocyte-bind g protein complete cDNA, complete eds, EST hits

Sequenced by LMU

Locus: /map="13"

Insert length: 3522 bp

Poly A stretch at pos. 3503, polyadenylation signal at pos. 3479

1 GGGGCGTGTT GGCGGGATTC TGAACGCTGC CATGGCTCAG ACCGTGTAGA 51 ATGTTACATT GTCGCTCACT CTGCCCATCA CGTGCCACAT TTGCTTGGGG

101 AAGGTACGTC AGCCTGTCAT ATGCATCAAC AACCATGTAT TTTGTTCGAT

151 TTGTATTGAT TTGTGGTTGA AGAATAATAG CCAGTGTCCA GCTTGCAGAG

201 TCCCCATCAC TCCTGAAAAT CCTTGCAAAG AAATTATAGG AGGAACAAGT

251 GAAAGTGAAC CTATGCTAAG CCATACGGTC AGGAAGCATC TTCGGAAAAC

301 TAGACTTGAA TTACTACACA AAGAATATGA GGACGAAATA GATTGTTTAC

351 AGAAAGAAGT AGAAGAGCTT AAGAGTAAAA ATCTCAGCTT GGAGTCACAG

401 ATCAAAGCTA TTCTGGATCC TTTAACCTTG GTGCAGGGCA ACCAAAATGA

451 AGACAAACAT CTAGTCACAG ATAATCCAAG TATAATTAAC CCAGAAACTG

501 TAGCAGAGTG GAAGAAAAAA CTCAGAACAG CTAATGAAAT CTATGAAAAA

551 GTGAAAGATG ATGTGGATAA GCTAAAGGAG GCAAATAAAA AATTGAAATT

601 GGAAAATGGT GGTCTGGTGA GGGAGAATTT ACGACTGAAG GCTGAAGTTG

651 ATAACAGATC ACCTCAAAAG TTTGGAAGGT TTGCAGTTGC TGCTCTTCAG

701 TCCAAAGTAG AACAGTATGA GCGTGAAACC AATCGCCTCA AGAAAGCCCT

751 GGAACGAAGT GATAAGTATA TAGAGGAACT AGAATCTCAA GTTGCACAGC

801 TAAAAAATTC AAGTGAAGAG AAAGAGGCTA TGAATTCCAT TTGCCAGACA

851 GCACTTTCTG CAGATGGCAA AGGGAGCAAA GGCAGTGAGG AGGATGTGGT

901 GTCAAAGAAT CAAGGCGATA GTGCCAGAAA GCAGCCTGGC TCATCCACCT

951 CCAGTTCTTC TCACCTAGCG AAGCCTTCCA GCAGCAGACT GTGTGACACC 1001 AGTTCTGCAA GGCAGGAAAG TACCAGCAAA GCAGACCTTA ACTGTTCTAA 1051 GAACAAAGAC CTATATCAAG AACAGGTAGA AGTAATGTTA GATGTGACAG 1101 ATACAAGTAT GGATACTTAT TTGGAAAGAG AATGGGGGAA TAAACCAAGT 1151 GACTGTGTAC CCTACAAAGA TGAAGAACTT TATGATTTTC CAGCTCCTTG 1201 TACTCCTTTG TCCCTTAGTT GCCTTCAGCT CAGTACTCCA GAAAATAGAG 1251 AGAGCTCTGT GGTCCAAGCA GGAGGTTCCA AAAAGCACTC AAACCATCTC 1301 AGAAAATTGG TGTTTGATGA TTTTTGTGAT TCTTCAAATG TTTCTAATAA 1351 AGATTCTTCA GAAGATGATA TAAGTAGAAG TGAAAATGAG AAGAAATCAG 1401 AATGTTTTTC TTCCACAAAG ACAGGATTTT GGGACTGTTG TTCCACAAGC 1451 TATGCCCAAA ACTTAGATTT TGAAAGTTCA GAGGGGAACA CGATAGCAAA 1501 TTCTGTTGGA GAAATATCTT CAAAATTGAG TGAGAAATCA GGCTTATGTT 1551 TATCCAAAAG GTTGAATTCT ATTCGCTCTT TTGAAATGAA CCGGACAAGA 1601 ACATCCAGTG AAGCATCGAT GGATGCTGCT TACCTTGACA AAATCTCTGA 1651 GTTGGATTCA ATGATGTCAG AGTCAGACAA CAGCAAGAGC CCTTGTAATA 1701 ACGGTTTTAA GTCACTGGAT TTGGATGGGT TATCAAAGTC ATCTCAAGGC 1751 AGTGAATTTC TTGAGGAACC TGATAAGTTG GAAGAAAAAA CTGAGCTAAA 1801 CCTTTCCAAA GGTTCTCTAA CTAATGATCA GTTAGAAAAT GGAAGTGAAT 1851 GGAAACCCAC TTCTTTTTTT TCTCCTCTCT CCATCTGACC AAGAAATGAA 1901 TGAAGATTTT TCACTCCATT CCAGTTCTTG TCCAGTAACT AATGAAATCA 1951 AACCCCCAAG CTGCTTGTTT CAGACAGAGT TTTCCCAGGG CATTTTGTTA 2001 AGCAGTTCAC ATCGACTATT GGAAGATCAA AGATTTGGGT CATCTTTGTT 2051 TAAGATGTCC TCAGAGATGC ACAGTCTTCA TAACCACCTT CAGTCTCCTT 2101 GGTCTACTTC CTTTGTGCCT GAAAAGAGGA ATAAAAATGT GAATCAATCA 2151 ACAAAAAGAA AAATCCAGAG CAGCCTTTCC AGTGCCAGCC CATCAAAAGC 2201 AACTAAAAGT TGACTCATTA GAAAGGTGTC ATTTGTGGTT TTGTCCTGAG 2251 AGAAATAGAA AAGTTGTTAA AGTTACCTTT TTTCCTCATA AAAGTTCTAT 2301 ACAAATTGGA ATTGATAATC TTTAGTCAAG TATCAAGTCA GGATGGTGGA 2351 TTAACCTGTA CCCAGAATAC TTATTGTTCA TTTTGAAAAG ACTTTGTTCT 2401 TTTCATTTTT ATTTGGGAGT CTTTGTGACC AGAGAAGTTA GGGAGGAGGT 2451 TATTTTTGTG TTTTGGGGTT GGTTGGTTGG TTGGTTTTGT TTTTGGTTTT 2501 GTTTTTTTAC TGAATTTGAT ATGTATCTCG GTTGGATATA CATTGTTTTT 2551 TTAAAAAATG TTATTTAACT GTTAGATACA GTGGCCTGTT GATAAGCCCC 2601 ACTTGTCTTC AGAACTTGGA TTTCTTAAAT AAAACTTTTA GTGTTGTCTA 2651 TACACTGCTC AATAAGACAC TTGAGTTTAA GCTTTTCCCA GGGTGGAAAT

2701 TATTTTACCT GTCCCTTTTT ATTTATGTTT AGTGATGGCC TAGTTTTTCT

2751 GCAGGGCCAT GATGGAGAAA TAGCACTCTA GCCTTAGTCC AATATTGATT

2801 TACTTTCTTT TTTTAGGTTT TATGTATATG TTTGCATTTT TTAGCATTGT

2851 GTTTTGTCCA GTTTTGTGAA AATGTTCTGC TAGTATGAAA GAAAACATTT

2901 TCTATATGAA GACATTTGTT TTATGTTAGG TAGCTTACAT TTTCTCCTCT

2951 GCGTGTGTGT GTATGTGTGT AAAATCAGAA ATTTAGCATA CTATGGAAAG

3001 AAGGCATGGA GCACTTGGGT TTAGAGGAAC CTAAAACATC ATAGCTTCAT

3051 TGTTCCAGAT GTAACAGGTT TGAAAGAGCT CATCGCCAAG TTCTTGATCC

3101 ACTTGCATTC CAGGGGAGTT CTCTTTTGAG TAGTATGTTT CTTGTTTGCA

3151 TGTTCCTGTT CTTTGTGGAA ACTATGCATG GTAGCATTTT TGCTTGCTGT

3201 GTTTTCCATA CTTAAGAAAA AGAGGTTTCA GTTGGCTGAT AGAATATCTT

3251 TTATGTAGGA CAAAACTTTT CTGTGAAGAG TGTTGAGGGG GTGAAGATAG

3301 GTAAGAGGTA AGCACAATTT TTAATTTAGG CTCTGAAAAA GTGTATTGTT

3351 CTAAACGTAT TTGGTATGCC TATATAGGTC TTTAAAAATG GGTTTGTATG

3401 CTGTTTAATG TGCACTGAAC ATTTTACATT AATATTGTAC TGTTTTACAT

3451 TAATACTGCA TGCTTTTCTA TGTGAATTGA ATAAAGAATG TCATAAGCAC

3501 TGGAAAAAAA AAAAAAAAAA AA

BLAST Results

Entry HSΘ58254 from database EMBL: human STS SHGC-11774. Score = 1643, P = 8.0e-67, identities = 345/355

Entry HS513217 from database EMBL: human STS SHGC-14656. Score = 1193, P = 5.8e-46, identities = 241/244

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 263 bp to 1885 bp; peptide length: 541 Category: similarity to known protein

1 MLSHTVRKHL RKTRLELLHK EYEDEIDCLQ KEVEELKSKN LSLESQIKAI 51 LDPLTLVQGN QNEDKHLVTD NPSIINPETV AEWKKKLRTA NEIYEKVKDD 101 VDKLKEANKK LKLENGGLVR ENLRLKAEVD NRSPQKFGRF AVAALQSKVE 151 QYERETNRLK KALERSDKYI EELESQVAQL KNSSEEKEAM NSICQTALSA 201 DGKGSKGSEE DVVSKNQGDS ARKQPGSSTS SSSHLAKPSS SRLCDTSSAR 251 QESTSKADLN CSKNKDLYQE QVEVMLDVTD TΞMDTYLERE WGNKPSDCVP 301 YKDEELYDFP APCTPLSLSC LQLSTPENRE SSVVQAGGSK KHSNHLRKLV 351 FDDFCDSSNV SNKDSSEDDI SRSENEKKSE CFSSTKTGFW DCCSTSYAQN 401 LDFESSEGNT IANSVGEISS KLSEKSGLCL SKRLNSIRSF EMNRTRTSSE 451 ASMDAAYLDK ISELDSMMSE SDNSKSPCNN GFKSLDLDGL SKSSQGSEFL 501 EEPDKLEEKT ELNLSKGSLT NDQLENGSEW KPTSFFSPLS I

BLASTP hits

Entry A42771 from database PIR: reticulocyte-binding protein 1 - Plasmodium vivax

Score = 127, P = 3.7e-08, identities = 68/300, positives = 145/300

Entry RBP1_PLAVB from database SWISSPROT: RETICULOCYTE BINDING PROTEIN 1 PRECURSOR. Score = 127, P = 3.9e-08, identities = 68/300, positives = 145/300

Entry MMDSPPG_1 from database TREMBL: gene: "DSPP"; product: "dentin sialophosphoprotein"; Mus musculus DSPP gene

Score = 160, P = 5.2e-08, identities = 87/373, positives = 146/373

Alert BLASTP hits for DKFZphfbr2_62nlO, frame 2 No Alert BLASTP hits found Pedant information for DKFZphfbr2_62nlO, frame 2

Report for DKFZphfbr2_62nlO .2

[LENGTH] 541

[MW] 60533.06

[pi] 5.10

[FUNCAT] 04.99 other transcription activities [S. cerevisiae, YKR092c] 3e-05

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKR092c] 3e-05

[PROSITE] LEUCINE ZIPPER 1

[PROSITE] MYRISTYL 7

[PROSITE] CAMP PHOSPHO SITE 1

[PROSITE] CK2 PHOSPHO SITE 18

[PROSITE] PROKAR LIPOPROTEIN 1

[PROSITE] TYR PHOSPHO SITE 1

[PROSITE] PKC PHOSPHO SITE 14

[PROSITE] ASN GLYCOSYLATION 7

[KW] All Alpha

[KW] LOW COMPLEXITY 9.24 %

[KW] COILED COIL 22.55 %

SEQ MLSHTVRKHLRKTRLELLHKEYEDEIDCLQKEVEELKSKNLSLESQIKAILDPLTLVQGN SEG PRD ccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhcccccccccc COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ QNEDKHLVTDNPSI INPETVAEWKKKLRTANEI EKVKDDVDKLKEANKKLKLENGGLVR

SEG xxxxxxxxxxxxxxxxxxxx

PRD cccceeeeeccccccccchhhhhhhhhhhhhhhhhhhhchhhhhhhhhhhhhhcccceee

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ ENLRLKAEVDNRSPQKFGRFAVAALQSKVEQYERETNRLKKALERSDKYIEELESQVAQL

SEG

PRD ehhhhhhhhcccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ KNSSEEKEAMNSICQTALSADGKGSKGSEEDVVSKNQGDSARKQPGSSTSSSSHLAKPSS

SEG xxxxxxxxxxxxxx

PRD hcchhhhhhhhhhhhhhhccccccccccceeeeecccccccccccccccccccccccccc

COILS CCCCCC

SEQ SRLCDTSSARQESTSKADLNCSKNKDLYQEQVEVMLDVTDTSMDTYLEREWGNKPSDCVP SEG x PRD ccccccccccccccccccccccccchhhhhhhhhcccccccccchhhhhhhccccccccc COILS

SEQ YKDEELYDFPAPCTPLSLSCLQLSTPENRESSVVQAGGSKKHSNHLRKLVFDDFCDSSNV SEG PRD cccccccccccccccccceeeecccccccceeeeeccccccccccccccccccccccccc COILS

SEQ SNKDSSEDDISRΞENEKKSECFSSTKTGFWDCCSTSYAQNLDFESSEGNTIANSVGEISS SEG PRD cccccccchhhhhccccccccccccccccccccccccccccccccccccccccccccccc COILS

SEQ KLSEKSGLCLSKRLNSIRSFEMNRTRTSSEASMDAAYLDKISELDSMMSESDNSKSPCNN SEG PRD ccccccccchhhhhcccccccccccchhhhhhhhhhhhhhhhhccccccccccccccccc COILS

SEQ GFKSLDLDGLSKSSQGSEFLEEPDKLEEKTELNLSKGSLTNDQLENGSEWKPTSFFSPLS SEG .. xxxxxxxxxxxxxxx PRD ccccccccccccccccceeecccchhhhhhhhhccccccccccccccccccccccccccc COILS

SEQ SEG PRD COILS

Prosite for DKFZphfbr2_62nlO .2

PS00001 40->44 ASN_GLYCOSYLATION PDOC00001 PS00001 182->186 ASN_GLYCOSYLATION PDOC00001 PS00001 260->264 ASN GLYCOSYLATION PDOC00001 PS00001 359->363 ASN_GLYCOSYLATION PDOC00001

PS00001 43->447 ASN_GLYCOSYLATION PDOC00001

PS00001 513->517 ASN_GLYCOSYLATION PDOC00001

PS00001 526->530 ASN_GLYCOSYLATION PDOC00001

PS00004 340->344 CAMP_PHOSPHO_SITE PDOC00004

PS00005 5->8 PKC_PHOSPHO_SITE PDOC00005

PS00005 156->159 PKC_PHOSPHO_SITE PDOC00005

PS00005 166->169 PKC_PHOSPHO_SITE PDOC00005

PS00005 220->223 PKC_PHOSPHO_SITE PDOC00005

PS00005 240->243 PKC_PHOSPHO_SITE PDOC00005

PS00005 248->251 PKC_PHOSPHO_SITE PDOC00005

PS00005 254->257 PKC_PHOSPHO_SITE PDOC00005

PS00005 339->342 PKC_PHOSPHO_SITE PDOC00005

PS00005 361->364 PKC_PHOSPHO_SITE PDOC00005

PS00005 384->387 PKC_PHOSPHO_SITE PDOC00005

PS00005 419->422 PKC_PHOSPHO_SITE PDOC00005

PS00005 423->426 PKC_PHOSPHO_SITE PDOC00005

PS00005 431->434 PKC_PHOSPHO_SITE PDOC00005

PS00005 436->439 PKC_PHOSPHO_SITE PDOC00005

PS00006 13->17 CK2_PHOSPHO_SITE PDOC00006

PS00006 79->83 CK2_PHOSPHO_SITE PDOC00006

PS00006 89->93 CK2_PHOSPHO_SITE PDOC00006

PS00006 147->151 CK2_PHOSPHO_SITE PDOC00006

PS00006 183->187 CK2_PHOSPHO_SITE PDOC00006

PS00006 208->212 CK2_PHOSPHO_SITE PDOC00006

PS00006 255->259 CK2_PHOSPHO_SITE PDOC00006

PΞ00006 281->285 CK2_PHOSPHO_SITE PDOC00006

PS00006 285->289 CK2_PHOSPHO_SITE PDOC00006

PS00006 324->328 CK2_PHOSPHO_SITE PDOC00006

PS0O006 361->365 CK2_PHOSPHO_SITE PDOC00006

PS00006 365->369 CK2_PHOSPHO_SITE PDOC00006

PS00006 371->375 CK2_PHOSPHO_SITE PDOC00006

PS00006 373->377 CK2_PHOSPHO_SITE PDOC00006

PS0O006 414->418 CK2_PHOSPHO_SITE PDOC00006

PS00006 447->451 CK2_PHOSPHO_SITE PDOC00006

PS00006 462->466 CK2_PHOSPHO_SITE PDOC00006

PS00006 469->473 CK2_PHOSPHO_SITE PDOC00006

PS00007 294->302 TYR_PHOSPHO_SITE PDOC00007

PS00008 204->210 MYRISTYL PDOC00008

PS00008 226->232 MYRISTYL PDOC00008

PS00008 292->298 MYRISTYL PDOC00008

PS00008 408->414 MYRISTYL PDOC00008

PS00008 427->433 MYRISTYL PDOC00008

PS00008 489->495 MYRISTYL PDOC00008

PS0O008 517->523 MYRISTYL PDOC00008

PS00013 310->321 PROKAR_LIPOPROTEIN PDOC00013

PS00029 104->126 LEUCINE ZIPPER PDOC00029

(No Pfam data available for DKFZphfbr2_62nlO .2)

DKFZphfbr2 62ol7

group: metabolism

DKFZphfbr2_62ol7.2 encodes a novel 282 ammo acid protein with weak similarity to the apolipoprotein E receptor.

The new protein contains a leucine zipper for protein-protein interaction, and three LDL- receptor class A domain (LDLRA_1) patterns. In LDL-receptors the class A domains form the binding site for LDL and calcium. The acidic residues between the fourth and sixth cysteines are important for high-affinity binding of positively charged sequences in LDLR's ligands.

The new protein can find application m modulation of cholesterol binding and transport by LDL-receptors and LDL-bmding proteins similarity to apolipoprotein E receptor complete cDNA, complete eds, start at Bp 56 matches kozak consensus ANCatg EST hits

Sequenced by LMU

Locus : unknown

Insert length: 1260 bp

Poly A stretch at pos. 1240, polyadenylation signal at pos. 1218

1 GGGGGATAAG AGAGCGGTCT GGACAGCGCG TGGCCGGCGC CGCTGTGGGG

51 ACAGCATGAG CGGCGGTTGG ATGGCGCAGG TTGGAGCGTG GCGAACAGGG

101 GCTCTGGGCC TGGCGCTGCT GCTGCTGCTC GGCCTCGGAC TAGGCCTGGA

151 GGCCGCCGCG AGCCCGCTTT CCACCCCGAC CTCTGCCCAG GCCGCAGGCC

201 CCAGCTCAGG CTCGTGCCCA CCCACCAAGT TCCAGTGCCG CACCAGTGGC

251 TTATGCGTGC CCCTCACCTG GCGCTGCGAC AGGGACTTGG ACTGCAGCGA

301 TGGCAGCGAT GAGGAGGAGT GCAGGATTGA GCCATGTACC CAGAAAGGGC

351 AATGCCCACC GCCCCCTGGC CTCCCCTGCC CCTGCACCGG CGTCAGTGAC

401 TGCTCTGGGG GAACTGACAA GAAACTGCGC AACTGCAGCC GCCTGGCCTG

451 CCTAGCAGGC GAGCTCCGTT GCACGCTGAG CGATGACTGC ATTCCACTCA

501 CGTGGCGCTG CGACGGCCAC CCAGACTGTC CCGACTCCAG CGACGAGCTC

551 GGCTGTGGAA CCAATGAGAT CCTCCCGGAA GGGGATGCCA CAACCATGGG

601 GCCCCCTGTG ACCCTGGAGA GCGTCACCTC TCTCAGGAAT GCCACAACCA

651 TGGGGCCCCC TGTGACCCTG GAGAGTGTCC CCTCTGTCGG GAATGCCACA

701 TCCTCCTCTG CCGGAGACCA GTCTGGAAGC CCAACTGCCT ATGGGGTTAT

751 TGCAGCTGCT GCGGTGCTCA GTGCAAGCCT GGTCACCGCC ACCCTCCTCC

801 TTTTGTCCTG GCTCCGAGCC CAGGAGCGCC TCCGCCCACT GGGGTTACTG

851 GTGGCCATGA AGGAGTCCCT GCTGCTGTCA GAACAGAAGA CCTCGCTGCC

901 CTGAGGACAA GCACTTGCCA CCACCGTCAC TCAGCCCTGG GCGTAGCCGG

951 ACAGGAGGAG AGCAGTGATG CGGATGGGTA CCCGGGCACA CCAGCCCTCA

1001 GAGACCTGAG CTCTTCTGGC CACGTGGAAC CTCGAACCCG AGCTCCTGCA

1051 GAAGTGGCCC TGGAGATTGA GGGTCCCTGG ACACTCCCTA TGGAGATCCG

1101 GGGAGCTAGG ATGGGGAACC TGCCACAGCC AGAACCGAGG GGCTGGCCCC

1151 AGGCAGCTCC CAGGGGGTAG GACGGCCCTG TGCTTAAGAC ACTCCTGCTG

1201 CCCCGTCTGA GGGTGGCGAT TAAAGTTGCT TCACATCCTC AAAAAAAAAA

1251 AAAAAAAAAC

BLAST Results

No BLAST result

Medlme entries

No Medline entry

Peptide information for frame 2

ORF from 56 bp to 901 bp; peptide length: 282

Category: similarity to known protein

Classi ication: unset

Prosite motifs: LDLRA_1 (67-90)

LDLRA_1 ( 67 -90 )

LDLRA 1 ( 145-168 ) LEUCINE ZIPPER (17-39)

1 MSGGWMAQVG AWRTGALGLA LLLLLGLGLG LEAAASPLST PTSAQAAGPS 51 SGSCPPTKFQ CRTSGLCVPL TWRCDRDLDC SDGSDEEECR IEPCTQKGQC 101 PPPPGLPCPC TGVSDCSGGT DKKLRNCSRL ACLAGELRCT LSDDCIPLTW 151 RCDGHPDCPD SSDELGCGTN EILPEGDATT MGPPVTLESV TSLRNATTMG 201 PPVTLESVPS VGNATSSΞAG DQSGSPTAYG VIAAAAVLSA SLVTATLLLL 251 SWLRAQERLR PLGLLVAMKE SLLLSEQKTS LP

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_62ol7, frame 2

TREMBL:AF110520_6 product: "NG29"; Mus musculus major histocompatibility complex region NG27, NG28, RPS28, NADH oxidoreductase, NG29, KIFCl, Fas-binding protein, BINGl, tapasin, RalGDS-like, KE2, BING4, beta 1, 3-galactosyl transferase, and RPS18 genes, complete eds; Sacm21 gene, partial eds; and unknown gene., N 1, Score = 733, P = 1.5e-72

PIR:JE0237 apolipoprotein E receptor 2 precursor mouse, N = 2, Score = 290, P = l.le-26

TREMBL:HSZ75190_1 product: "apolipoprotein E receptor 2 906";

H. sapiens mRNA for apolipoprotein E receptor 2, N = 1, Score = 279, P =

1.8e-23

>TREMBL:AF110520_6 product: "NG29"; Mus musculus major histocompatibility complex region NG27, NG28, RPS28, NADH oxidoreductase, NG29, KIFCl, Fas-bindmg protein, BINGl, tapasin, RalGDS-like, KE2, BING4, beta 1, 3-galactosyl transferase, and RPS18 genes, complete eds; Sacra21 gene, partial eds; and unknown gene. Length = 260

HSPs:

Score = 733 (110.0 bits) , Expect = 1.5e-72, P = 1.5e-72 Identities = 157/276 (56^! I, Positives = 178/276 (64%)

Query: 6 MAQVGAWRTGALGLALLLLLGLGLGLEAAASPLSTPTSAQAAGPSSGSCPPTKFQCRTSG 65 MA+ GA R ALGL L LL GL GLEAA +P T Q +G + SCP FQC TSG Sbjct: 1 MARGGAGRAVALGLVLRLLFGLRTGLEAAPAPAHT—RVQVSGSRADSCPTDTFQCLTSG 58 Query: 66 LCVPLTWRCDRDLDCSDGSDEEECRIEPCTQKGQCPPPPGLPCPCTGVSDCSGGTDKKLR 125

CVPL+WRCD D DCSDGSDEE+CRIE C Q GQC P LPC C +S CS +DK L Sbjct: 59 YCVPLSWRCDGDQDCSDGSDEEDCRIESCAQNGQCQPQSALPCSCDNISGCSDVSDKNL- 117 Query: 126 NCSRLACLAGELRCTLSDDCIPLTWRCDGHPDCPDSSDELGCGTNEILPEGDATTMGPPV 185 NCSR C EL C L D CIP TWRCDGHPDC DSSDEL C T+ Sbjct: 118 NCSRPPCQESELHCILDDVCIPHTWRCDGHPDCLDSSDELSCDTD T 163 Query: 186 TLESVTSLRNATTMGPPVTLESVPSVGNATSSSAGDQSGSPTAYGVIAAAAVLSASLVTA 245

++ + NATT T+E+ S N T +SAGD S +P+AYGVIAAA VLSA LV+A Sbjct: 164 EIDKIFQEENATTTRISTTMENETSFRNVTFTSAGDSSRNPSAYGVIAAAGVLSAILVSA 223 Query: 246 TLLLLSWLRAQERLRPLGLLVAMKESLLLSEQKTSL 281 TLL+L LR Q L P GLLVA+KESLLLSE+KTSL Sbjct: 224 TLLILLRLRGQGYLPPPGLLVAVKESLLLSERKTSL 259

Pedant information for DKFZphfbr2_62ol7, frame 2

Report for DKFZphfbr2_62ol7.2

[LENGTH] 282 [MW] 28991.19 [pi] 4.61 [HOMOL] TREMBL :AF110520_6 product: "NG29"; Mus musculus major histocompatibility complex region NG27, NG28, RPS28, NADH oxidoreductase, NG29, KIFCl, Fas-binding protein,

BINGl, tapasin, RalGDS-like, KE2, BING4, beta 1, 3-galactosyl transferase, and RPS18 genes, complete eds; Sacm21 gene, partial eds; and unknown gene. 5e-55

[BLOCKS] BL01209 LDL-receptor class A (LDLRA) domain proteins

[SCOP] dlaj 7.11.1.1.1 Ligand-binding domain of low-density lipoprotei 2e-10 [PIRKW] duplication le-19

['PIRKW] tandem repeat le-15

[PIRKW] heterodimer 6e-18

[PIRKW] endocytosis 4e-18

[PIRKW] heparan sulfate 2e-12

[PIRKW] VLDL le-19

[PIRKW] transmembrane protein le-19

[PIRKW] coated pits 4e-18

[PIRKW] fatty acid metabolism le-19

[PIRKW] G protein-coupled receptor le-10

[PIRKW] receptor le-19

[PIRKW] glycoprotein le-19

[PIRKW] lipid transport 4e-18

[PIRKW] LDL 5e-14

[PIRKW] calcium binding 6e-18

[PIRKW] extracellular protein 6e-13

[PIRKW] alternative splicing le-19

[PIRKW] extracellular matrix 3e-10

[PIRKW] chondroit sulfate proteoglycan 2e-12

[PIRKW] cholesterol 4e-18

[SUPFAM] leucine-rich alpha-2-glycoproteιn repeat homology le-10

[SUPFAM] LDL receptor YWTD-containing repeat homology le-19

[SUPFAM] trypsin homology 6e-13

[SUPFAM] alpha-2-macroglobulιn receptor 6e-18

[SUPFAM] LDL receptor le-19

[SUPFAM] LDL receptor ligand-binding repeat homology le-19

[SUPFAM] EGF homology le-19

[PROSITE] LDLRA_13

[PROSITE] LEUCINE_ZIPPER 1

[PFAM] Low-density lipoprotein receptor domain class A

[PFAM] TNFR/NGFR cysteine-rich region

[KW] SIGNAL_PEPTIDE 31

[KW] TRANSMEMBRANE 1

[KW] LOW_COMPLEXITY 22.34 %

SEQ MSGGWMAQVGAWRTGALGLALLLLLGLGLGLEAAASPLSTPTSAQAAGPSSGSCPPTKFQ SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccccccccchhhhhhhhhhhhhhhhhhhhhhhhccccccccccccccccccccceee

MEM

SEQ CRTSGLCVPLTWRCDRDLDCSDGSDEEECRIEPCTQKGQCPPPPGLPCPCTGVSDCSGGT SEG xxxxxxxxxxx PRD ecccccceeeeecccccccccccccccccccccccccccccccccccccccccccccccc MEM

SEQ DKKLRNCSRLACLAGELRCTLSDDCIPLTWRCDGHPDCPDSSDELGCGTNEILPEGDATT SEG PRD cccccccccccccccceeeccccccccccccccccccccccccccccccccccccccccc MEM

SEQ MGPPVTLESVTSLRNATTMGPPVTLESVPSVGNATSSSAGDQSGSPTAYGVIAAAAVLSA

SEG xxxxxxxx

PRD ccccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhh

MEM MMMMMMM

SEQ SLVTATLLLLSWLRAQERLRPLGLLVAMKESLLLSEQKTSLP

SEG xxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhcccccc

MEM MMMMMMMMMM

Prosite for DKFZphfbr2_62ol7.2

PS01209 67->90 LDLRA_1 PDOC00929 PΞ01209 67->90 LDLRA_1 PDOC00929 PS01209 145->168 LDLRA_1 PDOC00929 PS00029 17->39 LEUCINE ZIPPER PDOC00029

Pfam for DKFZphfbr2_62ol7.2

HMM_NAME TNFR/NGFR cysteine-rich region

HMM *CpeGtYtD.WNHvpqClpC.trCePEMGQYMvqPCTwTQNT.VC*

CP+ ++ + + C+P RC+ ++ +C + ++ +C Query 54 CPPTKFQCRTS--GLCVPLTWRCDR—DL DCSDGSDEEEC 89 HMM_NAME Low-density lipoprotein receptor domain class A

HMM *tTCeGPDEFQCgSGeMRCIPMsWvCDGDpDCeDWSDEWPeNChp*

C P +FQC+++ C+P+ W+CD D DC D+SDE E+C+ Query 52 GSCP-PTKFQCRTSG-LCVPLTWRCDRDLDCSDGSDE—EECRI 91

54.99 (bits) f: 130 t: 169 Target: dkfzphfbr2_62ol7.2 similarity to apolipoprotein E receptor

Alignment to HMM consensus: Query *tTCeGPDEFQCgSGeMRCIPMsWvCDGDpDCeDWSDEWPeNChp*

C + E +C + CIP+ W+CDG PDC D SDE ++C+ dkfzphfbr2 130 LACL-AGELRCTLΞD-DCIPLTWRCDGHPDCPDSSDE—LGCGT 169

DKFZphfbr2_64al5 group: nucleic acid management

DKFZphfbr2_64al5 encodes a novel 255 amino acid protein with strong similarity to inorganic pyrophosphatases

Inorganic pyrophosphatase (EC 3.6.1.1) (PPase) is the enzyme responsible for the hydrolysis of pyrophosphate (PPi) which is formed as the product of the many biosynthetic reactions that utilize ATP. All known PPases require the presence of divalent metal cations, with magnesium conferring the highest activity.

The new protein can find application as a new enzyme for biotechnologic processes. strong similarity to inorganic pyrophosphatases unspliced Intron 212-256 see EST HS1190948

Sequenced by Qiagen

Locus : unknown

Insert length: 1188 bp

Poly A stretch at pos. 1170, polyadenylation signal at pos. 1151

1 GGGGGTTGGG GACCAGTGCA GGGACCGGGT CGCGCCGTGC TATGGCCCTG

51 TACCACACTG AGGAGCGCGG CCAGCCCTGC TCGCAGAATT ACCGCCTCTT

101 CTTTAAGAAT GTAACTGGTC ACTACATTTC CCCCTTTCAT GATATTCCTC

151 TGAAGGTGAA CTCTAAAGAG GACACTGAGG CTCAAGGCAT TTTTATAGAC

201 TTGTCTAAGA TCTGGAAAAT GGCATTCCTA TGAAGAAAGC ACGAAATGAT

251 GAATATGAGA ATCTGTTTAA TATGATTGTA GAAATACCTC GGTGGACAAA

301 GGCTAAAATG GAGATTGCCA CCAAGGAGCC AATGAATCCC ATTAAACAAT

351 ATGTAAAGGA TGGAAAGCTA CGCTATGTGG CGAATATCTT CCCTTACAAG

401 GGTTATATAT GGAATTATGG TACCCTCCCT CAGACTTGGG AAGATCCCCA

451 TGAAAAAGAT AAGAGCACGA ACTGCTTTGG AGATAATGAT CCTATTGATG

501 TTTGCGAAAT AGGCTCAAAG ATTCTTTCTT GTGGAGAAGT TATTCATGTG

551 AAGATCCTTG GAATTTTGGC TCTTATTGAT GAAGGTGAAA CAGATTGGAA

601 ATTAATTGCT ATCAATGCGA ATGATCCTGA AGCCTCAAAG TTTCATGATA

651 TTGATGATGT TAAGAAGTTC AAACCGGGTT ACCTGGAAGC TACTCTTAAT

701 TGGTTTAGAT TATGTAAGGT ACCAGATGGA AAACCAGAAA ACCAGTTTGC

751 TTTTAATGGA GAATTCAAAA ACAAGGCTTT TGCTCTTGAA GTTATTAAAT

801 CCACTCATCA ATGTTGGAAA GCATTGCTTA TGAAGAACTG TAATGGAGGA

851 GCTACAAATT GCACAAACGT GCAGATATCT GATAGCCCTT TCCGTTGCAC

901 TCAAGAGGAA GCAAGATCAT TAGTTGAATC GGTATCATCT TCACCAAATA

951 AAGAAAGTAA TGAAGAAGAG CAAGTGTGGC ACTTCCTTGG CAAGTGATTG

1001 AAACATCTGA AATTCTGCTG TCAAGATTCC CATCTCTAAG GACTCCAAGA

1051 CTCTTTTTCC CCAAGTGCTA GAGACAAGGG GGTCTATGAG CATTTACTGA

1101 CTTCCTGTTA AAACTTCATT TTTTCAAACT TTTTGAGCTA TGCAATATAT

1151 AAATAAACAG TAAGAATTTT AAAAAAAAAA AAAAAAAA

BLAST Results

Entry HSPPASEMR from database EMBL:

H. sapiens partial mRNA for pyrophosphatase.

Score = 1706, P = 1.6e-70, identities = 342/343

Medlme entries

No Medline entry

Peptide information for frame 2

ORF from 230 bp to 994 bp; peptide length: 255 Category: strong similarity to known protein Classification: unset Prosite motifs: PPASE (85-92) 1 MKKARNDEYE NLFNMIVEIP RWTKAKMEIA TKEPMNPIKQ YVKDGKLRYV 51 ANIFPYKGYI WNYGTLPQTW EDPHEKDKST NCFGDNDPID VCEIGSKILS 101 CGEVIHVKIL GILALIDEGE TDWKLIAINA NDPEASKFHD IDDVKKFKPG 151 YLEATLNWFR LCKVPDGKPE NQFAFNGEFK NKAFALEVIK STHQCWKALL 201 MKNCNGGATN CTNVQISDSP FRCTQEEARS LVEΞVSSSPN KESNEEEQVW 251 HFLGK

BLASTP hits

Entry IPYR_KLULA from database SWISSPROT:

INORGANIC PYROPHOSPHATASE (EC 3.6.1.1) (PYROPHOSPHATE PHOSPHO-

HYDROLASE) (PPASE) .

Score = 689, P = 6.0e-68, identities = 128/248, positives = 170/248

Entry A45153 from database PIR: inorganic pyrophosphatase (EC 3.6.1.1) - bovine

Score = 862, P = 2.8e-86, identities = 146/226, positives = 190/226

Entry AF085600_1 from database TREMBLNEW: gene: "Nurf-38"; product: "inorganic pyrophosphatase NURF-38";

Drosophila melanogaster inorganic pyrophosphatase NURF-38 (Nurf-38) gene, complete eds.

Score = 731, P = 2.1e-72, identities = 134/248, positives = 177/248

Entry PWBY from database PIR: inorganic pyrophosphatase (EC 3.6.1.1) - yeast (Saccharomyces cerevisiae)

Score = 688, P = 7.7e-68, identities = 133/251, positives = 174/251

Alert BLASTP hits for DKFZphfbr2_64al5, frame 2

SWISSPROT: I PYR_DROME INORGANIC PYROPHOSPHATASE (EC 3.6.1.1) (PYROPHOSPHATE PHOSPHO- HYDROLASE) (PPASE)., N = 1, Score = 731, P = 2.4e-72

>SWISSPROT:IPYR_DROME INORGANIC PYROPHOSPHATASE (EC 3.6.1.1) (PYROPHOSPHATE PHOSPHO- HYDROLASE) (PPASE) . Length = 290

HSPs:

Score = 731 (109.7 bits), Expect = 2.4e-72, P = 2.4e-72 Identities = 134/248 (54%), Positives = 177/248 (71%)

Query: 7 DEYENLFNMIVEIPRWTKAKMEIATKEPMNPIKQYVKDGKLRYVANIFPYKGYIWNYGTL 66

+E + ++NM+VE+PRWT AKMEI+ K PMNPIKQ +K GKLR+VAN FP+KGYIWNYG L Sbjct: 40 NEEKTIYNMVVEVPRWTNAKMEISLKTPMNPIKQDIKKGKLRFVANCFPHKGYIWNYGAL 99

Query: 67 PQTWEDPHEKDKSTNCFGDNDPIDVCEIGSKILSCGEVIHVKILGILALIDEGETDWKLI 126

PQTWE+P + ST C GDNDPIDV EIG ++ G+V+ VK+LG ALIDEGETDWK+I Sbjct: 100 PQTWENPDHIEPSTGCKGDNDPIDVIEIGYRVAKRGDVLKVKVLGQFALIDEGETDWKII 159

Query: 127 AINANDPEASKFHDIDDVKKFKPGYLEATLNWFRLCKVPDGKPENQFAFNGEFKNKAFAL 186

AI+ NDP ASK +DI DV ++ PG L AT+ WF++ K+PDGKPENQFAFNG+ KN FA Sbjct: 160 AIDVNDPLASKVNDIADVDQYFPGLLRATVEWFKIYKIPDGKPENQFAFNGDAKNADFAN 219

Query: 187 EVIKSTHQCWKALLMKNCNGGATNCTNVQISDSPFRCTQEEARS-LVESVSSSPNKESNE 245

+1 TH+ W+ L+ ++ G+ + TN+ +S +EEA L E+ +E ++ Sbjct: 220 TIIAETHKFWQNLVHQSPASGSISTTNITNRNSEHVIPKEEAEKILAEAPDGGQVEEVSD 279

Query: 246 EEQVWHFL 253

WHF+ Sbjct: 280 TVDTWHFI 287

Peptide information for frame 3

ORF from 42 bp to 230 bp; peptide length: 63 Category: strong similarity to known protein Classification: unset

1 MALYHTEERG QPCSQNYRLF FKNVTGHYIS PFHDIPLKVN SKEDTEAQGI 51 FIDLSKIWKM AFL BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_64al5, frame 3

SWISSPROT :IPYR_DROME INORGANIC PYROPHOSPHATASE (EC 3.6.1.1) (PYROPHOSPHATE PHOSPHO- HYDROLASE) (PPASE)., N = 1, Score = 118, P = 8.8e-07

PIR:A45153 inorganic pyrophosphatase (EC 3.6.1.1) - bovine, N = 1, Score = 113, P = 3. le-06

TREMBLNEW:AF108211_1 product: "cytosolic inorganic pyrophosphatase"; Homo sapiens cytosolic inorganic pyrophosphatase mRNA, partial eds., N = 1, Score = 106, P = 1.8e-05

>SWISSPROT:IPYR_DROME INORGANIC PYROPHOSPHATASE (EC 3.6.1.1) (PYROPHOSPHATE PHOSPHO- HYDROLASE) (PPASE) . Length = 290

HSPs:

Score = 118 (17.7 bits), Expect = 8.8e-07, P = 8.8e-07 Identities = 23/43 (53%), Positives = 29/43 (67%)

Query: 1 MALYHTEERGQPCSQNYRLFFKNVTGHYISPFHDIPLKVNSKE 43

MALY T E+G Ξ +Y L+FKN G+ ISP HDIPL N ++ Sbjct: 1 MALYETVEKGAKNSPSYSLYFKNKCGNVISPMHDIPLYANEEK 43

Pedant information for DKFZphfbr2_64al5, frame 2

Report for DKFZphfbr2_64al5.2

[LENGTH] 255

[MW] 29177.34

[pi] 5.67

[HOMOL] TREMBLNEW: AF108211_1 product: "cytosolic inorganic pyrophosphatase"; Homo sapiens cytosolic inorganic pyrophosphatase mRNA, partial eds. 2e-93

[FUNCAT] 01.04.01 phosphate utilization [S. cerevisiae, YBROllc] 9e-73

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YBROllc] 9e-73

[FUNCAT] 02.99 other energy generation activities [S. cerevisiae, YMR267w] le-58

[FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YMR267w] le-58

[FUNCAT] 1 genome replication, transcription, recombination and repair [M. genitalium, MG351] le-06

[FUNCAT] g carbohydrate metabolism and transport [H. influenzae, HI0124] 2e-06

[BLOCKS] BL00387D

[BLOCKS] BL00387C

[BLOCKS] BL00387B

[BLOCKS] BL00387A

[SCOP] dlwgja_ 2.29.5.1.1 Inorganic pyrophosphatase [baker's yeas le-113

[EC] 3.6.1.1 Inorganic pyrophosphatase 7e-92

[PIRKW] mitochondrion 3e-57

[PIRKW] hydrolase 7e-92

[PIRKW] homodimer 2e-71

[SUPFAM] inorganic pyrophosphatase 7e-92

[PROSITE PPASE 1

[KW] Alpha_Beta

[KW] 3D

[KW] LOW COMPLEXITY 6.27 %

SEQ MKKARNDEYENLFNMIVEIPRWTKAKMEIATKEPMNPIKQYVKDGKLRYVANIFPYKGYI SEG lhukB EGGGCEEEEEEEETTTbCBCEEETTTTTTTCEEECEETTEECBCCBBTTBTTbT

SEQ WNYGTLPQTWEDPHEKDKSTNCFGDNDPIDVCEIGSKILSCGEVIHVKILGILALIDEGE SEG lhukB CEEEETTTTCBTTTTEETTTTEECCCBCCEEEECCCCCCTTTEEEEEEEEEEEEETTTTB

SEQ TDWKLIAINANDPEASKFHDIDDVKKFKPGYLEATLNWFRLCKVPDGKPENQFAFNGEFK SEG lhukB CEEEEEEEETTTTTGGGCCCHHHHHHHTTTHHHHHHHHHHHHCGGGCCCCCCBCGGGCCB

SEQ NKAFALEVIKSTHQCWKALLMKNCNGGATNCTNVQISDSPFRCTQEEARSLVESVSSSPN SEG xxxxxxxxx lhukB CHHHHHHHHHHHHHHHHHHHHCTTTTTTTCCCBTTTTTTT SEQ KESNEEEQVWHFLGK

SEG xxxxxxx lhukB

Prosite for DKFZphfbr2_64al5.2 PS00387 85->92 PPASE PDOC00325

(No Pfam data available for DKFZphfbr2_64al5.2)

Pedant information for DKFZphfbr2_64al5, frame 3

Report for DKFZphfbr2_64al5.3

[LENGTH] 63

[MW] 7405.54

[pi] 6.81

[HOMOL] SWISSPROT :IPYR_DROME INORGANIC PYROPHOSPHATASE (EC 3.6.1.1) (PYROPHOSPHATE

PHOSPHO- HYDROLASE) (PPASE) . le-06

[EC] 3.6.1.1 Inorganic pyrophosphatase 5e-06

[PIRKW] hydrolase 5e-06

[SUPFAM] inorganic pyrophosphatase 5e-06

[KW] All_Beta

SEQ MALYHTEERGQPCSQNYRLFFKNVTGHYISPFHDIPLKVNSKEDTEAQGIFIDLSKIWKM PRD cccccccccccccccceeeeeecccccccccccccccccccccccccceeeechhhhhhh

SEQ AFL PRD CCC

(No Prosite data available for DKFZphfbr2_64al5.3) (No Pfam data available for DKFZphfbr2_64al5.3)

DKFZphfbr2_64cl6

group: brain derived

DKFZphfbr2_64al6.2 encodes a novel 101 amino acid protein without similarity to known proteins .

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of bram-specific genes . unknown complete cDNA, complete eds, EST hits

Sequenced by Qiagen

Locus: /map="745_A_2; 756_F_2; 842_C_2"

Insert length: 1866 bp

Poly A stretch at pos. 1848, polyadenylation signal at pos. 1829

1 GGGCGCGGCG CCGGAGGAGG AAGTGGTGAG GTTGTTGCTC CTTCAGCGCC 51 TATCGCTGGC TCTTGGGGCG CAGAGAGGGG CCGCAGTCTC CGCGGCTGCG

101 TCGAGCTCCC TTGCAGTCCC CTCCATGTTC CCCGGCGCCA CTACTCCCCT

151 TCCTAAGGCC GCCGCTTACC CCGGGGTCTA TGGAAGTAAT GGAAGGACCC

201 CTCAACCTGG CTCATCAACA GAGCAGACGA GCAGACCGTT TATTAGCTGC

251 AGGCAAATAC GAAGAGGCTA TTTCTTGTCA CAAAAAGGCT GCAGCATATC

301 TTTCTGAAGC CATGAAGCTG ACACAGTCAG AGCAGGCTCA TCTTTCACTG

351 GAATTGCAAA GGGATAGCCA TATGAAACAG CTCCTCCTCA TCCAAGAGAG

401 ATGGAAAAGG GCCCAGCGTG AAGAAAGATT GAAAGCCCAG CAGAACACAG

451 ACAAGGATGC AGCTGCCCAT CTTCAGACAT CTCACAAACC CTCTGCAGAG

501 GATGCAGAGG GCCAGAGTCC CCTTTCTCAG AAGTACAGCC CTTCCACAGA

551 GAAATGCCTG CCTGAGATTC AGGGGATCTT TGACAGGGAT CCAGACACAC

601 TACTTTATTT ACTTCAGCAA AAGAGTGAGC CAGCAGAGCC ATGTATTGGA

651 AGCAAAGCCC CAAAAGATGA TAAAACAATT ATAGAGGAGC AGGCAACCAA

701 AATTGCAGAT TTGAAGAGGC ATGTGGAATT CCTTGTGGCT GAGAATGAAA

751 GATTAAGGAA AGAAAATAAA CAACTAAAGG CTGAAAAGGC CAGACTTCTA

801 AAAGGTCCAA TAGAAAAGGA GCTGGATGTA GATGCTGATT TTGTAGAAAC

851 GTCAGAGTTA TGGAGCTTGC CACCACATGC AGAAACTGCT ACAGCCTCCT

901 CAACCTGGCA GAAGTTCGCA GCAAATACTG GGAAAGCCAA GGACATTCCA

951 ATCCCCAATC TTCCTCCCTT GGATTTTCCA TCTCCAGAAC TTCCTCTTAT 1001 GGAGCTCTCT GAGGATATTC TGAAAGGACT TATGAATAAT TAAAATGGAA 1051 GGCCACAGAA AAGGGGAAAA GAGGAAATAA TACAGTAATC GTTAATCCAG 1101 CAAAAAGAAA TGAAAAGGGA AAACCACATA GAAGGGTAAT CCCGGAAATG 1151 CTTCATCTGG TGGACTGTGG GAGCAGAGGC ATTGCCAGGA CTTGGGAAAC 1201 AGTCACTGTG AAATGCGCTG CGTATCTCAT TCACTCACTT CAGCTAATGA 1251 CTCCGACTTG GCAGACGCTA AACTCATGGA GGTTCGGTTT CTCCTGATAC 1301 AAACCAAATG GCTACCTGGA AGAATTTCTT TCAAGCAACA GTTATTTTTC 1351 TTATCTTCAG GGTTAAAATG TATAAAAGTT ATGTGTAATT AATCTATAAT 1401 GCCATAAATG ATAATGCAAA ACCTAAATAA TATGGTGGCC GGAGGGGCTG 1451 CCTTATATTT GAAACATGCT TTCTATCATG CATTGACTGT ATGCATTTTG 1501 TTAATGCACA TTCTGTTTGT TTAAGGTGTG TGAGATACAC ACCTTTCTAG 1551 ATGAAACTAT ATGTGCCACA CTTTGCACTA CTCATAATGA TAACCTCAAG 1601 ACTATCAGAA GAAATATTTA AATTTCCATT TTATGAAGAA AGGAACCAAA 1651 TTATTATGCT TTTTAAAACA AATTACCAGT TTACATAATT AATCAGGGTG 1701 CATTTTAAGT TCTAACTTCG TTTATTGTAT AATGCATCAT TTGAAAATAC 1751 CAAGGAGGAA ATACCCTTTG TTTTTAATGA TGCAAGAGTG GACGTAATGC 1801 TAGTTGGCAG TATTTTATTG TAAGAAATCA ATAAAGTAAT TGTGTTTTAA 1851 AAAAAAAAAA AAAAAA

BLAST Results

Entry HS286143 from database EMBL: human STS WI-6844. Score = 1460, P = 3.4e-61, identities = 292/292

Medline entries

No Medline entry Peptide information for. frame 2

ORF from the beginning to 304 bp; peptide length: 102 Category: questionable ORF Classification: unset

1 GAAPEEEVVR LLLLQRLSLA LGAQRGAAVS AAASSSLAVP SMFPGATTPL 51 PKAAAYPGVY GSNGRTPQPG SSTEQTSRPF ISCRQIRRGY FLSQKGCSIS 101 F

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_64cl6, frame 2

No Alert BLASTP hits found

Peptide information for frame 3

ORF from 180 bp to 1040 bp; peptide length: 287

Category: putative protein

Classification: unset

Prosite motifs: LEUCINE_ZIPPER (178-200)

LEUCINE ZIPPER (185-207)

1 MEVMEGPLNL AHQQΞRRADR LLAAGKYEEA ISCHKKAAAY LSEAMKLTQS

51 EQAHLSLELQ RDSHMKQLLL IQERWKRAQR EERLKAQQNT DKDAAAHLQT

101 SHKPSAEDAE GQSPLSQKYS PSTEKCLPEI QGIFDRDPDT LLYLLQQKSE

151 PAEPCIGSKA PKDDKTIIEE QATKIADLKR HVEFLVAENE RLRKENKQLK

201 AEKARLLKGP IEKELDVDAD FVETSELWSL PPHAETATAS STWQKFAANT

251 GKAKDIPIPN LPPLDFPSPE LPLMELSEDI LKGLMNN

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_64cl6, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_64cl6, frame 2

Report for DKFZphfbr2_64cl6.2

[LENGTH] 101

[MW] 10469.94

[pi] 10.18

[KW] All Alpha

[KW] LOW COMPLEXITY 29.70 %

SEQ GAAPEEEVVRLLLLQRLSLALGAQRGAAVSAAASSSLAVPSMFPGATTPLPKAAAYPGVY

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD ccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccccccccccccccccc

SEQ GSNGRTPQPGSSTEQTSRPFISCRQIRRGYFLSQKGCSISF

SEG

PRD ccccccccccccccccccccchhhhhccccccccccccccc

(No Prosite data available for DKFZphfbr2_64cl6.2) (No Pfam data available for DKFZphfbr2_64cl6.2)

Pedant information for DKFZphfbr2 64cl6, frame 3 Report for DKFZphfbr2_64cl6.3

[LENGTH] 287

[MW] 32343.79

[pi] 5.61

[PROSITE] LEUCINE ZIPPER 2

[KW] All Alpha

[KW] COILED COIL 14.98 %

SEQ MEVMEGPLNLAHQQSRRADRLLAAGKYEEAISCHKKAAAYLSEAMKLTQSEQAHLSLELQ

PRD ccccchhhhhhhhhhhhhhhhhhhcchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS

SEQ RDSHMKQLLLIQERWKRAQREERLKAQQNTDKDAAAHLQTSHKPSAEDAEGQSPLSQKYS

PRD hhcchhhhhhhhhhhhhhhhhhhhhhhhccccchhhhhhhcccccccccccccccccccc

COILS

SEQ PSTEKCLPEIQGIFDRDPDTLLYLLQQKSEPAEPCIGSKAPKDDKTIIEEQATKIADLKR

PRD cccccccchhhhhcccccchhhhhhhhhcccccccccccccccchhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCC

SEQ HVEFLVAENERLRKENKQLKAEKARLLKGPIEKELDVDADFVETSELWSLPPHAETATAS

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccccccccccccccccccccccc

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ STWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGLMNN

PRD hhhhhhhhhcccccccccccccccccccccchhhhhhhhhhhhhccc

COILS

Prosite for DKFZphfbr2_64cl6.3

PS00029 178->200 LEUCINE_ZIPPER PDOC00029 PS00029 185->207 LEUCINE ZIPPER PDOC00029

(No Pfam data available for DKFZphfbr2_64cl6.3)

DKFZphfbr2 64c4

group: brain derived

DKFZphfbr2_64c4 encodes a novel 467 ammo acid protein with similarity to A. thaliana T08H3.5

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of bram-specific genes .

similarity to A. thaliana T08I13.5 complete cDNA, complete eds, EST hits on genomic level encoded by AC005043 11 exons

Sequenced by Qiagen

Locus : unknown

Insert length: 1559 bp

Poly A stretch at pos. 1540, no polyadenylation signal found

1 TGGGACCGCC GGAAGTTTCT GCCGCGGCTT TGCGGGGACG GGGGAGTGGT

51 AGTGGGGGCT GCAGCTGCCG GACCCAGGCG CGATGGCTAC GGGCGCGGAT

101 GTACGGGACA TTCTAGAACT CGGGGGTCCA GAAGGGGATG CAGCCTCTGG

151 GACCATCAGC AAGAAGGACA TTATCAACCC GGACAAGAAA AAATCCAAGA

201 AGTCCTCTGA GACACTGACT TTCAAGAGGC CCGAGGGCAT GCACCGGGAA

251 GTCTATGCCT TGCTCTACTC TGACAAGAAG GATGCACCCC CACTGCTACC

301 CAGTGACACT GGCCAGGGAT ACCGTACAGT GAAGGCCAAG TTGGGCTCCA

351 AGAAGGTGCG GCCTTGGAAG TGGATGCCAT TCACCAACCC GGCCCGCAAG

401 GACGGAGCAA TGTTCTTCCA CTGGCGACGT GCAGCGGAGG AGGGCAAGGA

451 CTACCCCTTT GCCAGGTTCA A AAGACTGT GCAGGAGCCT GTGTACTCGG

501 AGCAGGAGTA CCAGCTTTAT CTCCACGATA ATGCTTGGAC TAAGGCAGAA

551 ACTGACCACC TCTTTGACCT CAGCCGCCGC TTTGACCTGC GTTTTGTTGT

601 TATCCATGAC CGGTATGACC ACCAGCAGTT CAAGAAGCGT TCTGTGGAAG

651 ACCTGAAGGA GCGGTACTAC CACATCTGTG CTAAGCTTGC CAACGTGCGG

701 GCTGTGCCAG GCACAGACCT TAAGATACCA GTATTTGATG CTGGGCACGA

751 ACGACGGCGG AAGGAACAGC TTGAGCGTCT CTACAACCGG ACCCCAGAGC

801 AGGTGGCAGA GGAGGAGTAC CTGCTACAGG AGCTGCGCAA GATTGAGGCC

851 CGGAAGAAGG AGCGGGAGAA ACGCAGCCAG GACCTGCAGA AGCTGATCAC

901 AGCGGCAGAC ACCACTGCAG AGCAGCGGCG CACGGAACGC AAGGCCCCCA

951 AAAAGAAGCT ACCCCAGAAA AAGGAGGCTG AGAAGCCGGC TGTTCCTGAG

1001 ACTGCAGGCA TCAAGTTTCC AGACTTCAAG TCTGCAGGTG TCACGCTGCG

1051 GAGCCAACGG ATGAAGCTGC CAAGCTCTGT GGGACAGAAG AAGATCAAGG

1101 CCCTGGAACA GATGCTGCTG GAGCTTGGTG TGGAGCTGAG CCCGACACCT

1151 ACGGAGGAGC TGGTGCACAT GTTCAATGAG CTGCGAAGCG ACCTGGTGCT

1201 GCTCTACGAG CTCAAGCAGG CCTGTGCCAA CTGCGAGTAT GAGCTGCAGA

1251 TGCTGCGGCA CCGTCATGAG GCACTGGCCC GGGCTGGTGT GCTAGGGGGC

1301 CCTGCCACAC CAGCATCAGG CCCAGGCCCG GCCTCTGCTG AGCCGGCAGT

1351 GTCTGAACCC GGACTTGGTC CTGACCCCAA GGACACCATC ATTGATGTGG

1401 TGGGCGCACC CCTCACGCCC AATTCGAGAA AGCGACGGGA GTCGGCCTCC

1451 AGCTCATCTT CCGTGAAGAA AGCCAAGAAG CCGTGAGAGG CCCCACGGGG

1501 TGTGGGCGAC GCTGTTATGT AAATAGAGCT GCTGAGTTGG AAAAAAAAAA

1551 AAAAAAAAA

BLAST Results

Entry AC005043 from database EMBL:

Homo sapiens clone NH0576N21; HTGS phase 1, 5 unordered pieces.

Score = 1506, P = 4.6e-244, identities = 316/330

Medl e entries No Medline entry

Peptide information for frame 2 ORF from 83 bp to 1483 bp; peptide length: 467 Category: similarity to unknown protein

1 MATGADVRDI LELGGPEGDA ASGTISKKDI INPDKKKSKK SSETLTFKRP 51 EGMHREVYAL LYSDKKDAPP LLPSDTGQGY RTVKAKLGΞK KVRPWKWMPF 101 TNPARKDGAM FFHWRRAAEE GKDYPFARFN KTVQEPVYSE QEYQLYLHDN 151 AWTKAETDHL FDLΞRRFDLR FVVIHDRYDH QQFKKRSVED LKERYYHICA 201 KLANVRAVPG TDLKIPVFDA GHERRRKEQL ERLYNRTPEQ VAEEEYLLQE 251 LRKIEARKKE REKRSQDLQK LITAADTTAE QRRTERKAPK KKLPQKKEAE 301 KPAVPETAGI KFPDFKSAGV TLRSQRMKLP SSVGQKKIKA LEQMLLELGV 351 ELSPTPTEEL VHMFNELRSD LVLLYELKQA CANCEYELQM LRHRHEALAR 401 AGVLGGPATP ASGPGPASAE PAVSEPGLGP DPKDTIIDVV GAPLTPNSRK 451 RRESASSSSS VKKAKKP

BLASTP hits

Entry ATAC2337_5 from database TREMBLNEW: gene: "T08I13.5"; Arabidopsis thaliana chromosome II BAC T08I13 genomic sequence, complete sequence.

Score = 340, P = 2.6e-30, identities = 115/374, positives = 176/374

Entry YE8D_SCHPO from database SWISSPROT:

HYPOTHETICAL 47.1 KD PROTEIN C9G1.13C IN CHROMOSOME I.

Score = 221, P = 1.9e-20, identities = 67/192, positives = 97/192

Entry S64291 from database PIR: hypothetical protein YGR002c - yeast (Saccharomyces cerevisiae)

Score = 202, P = 2.8e-13, identities = 71/260, positives = 124/260

Alert BLASTP hits for DKFZphfbr2_64c4, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_64c4, frame 2

Report for DKFZphfbr2_64c4.2

[LENGTH] 467

[MW] 53007.60

[pi] 9.51

[HOMOL] TREMBL :ATAC2337_5 gene: "T08I13.5"; Arabidopsis thaliana chromosome II BAC

T08I13 genomic sequence, complete sequence. 4e-29

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YGR002c] le-19

[PROSITE] MYRISTYL 1

[PROSITE] CAMP_PHOSPHO_SITE 4

[PROSITE] CK2_PHOSPHO_SITE 10

[PROSITE] TYR_PHOSPHO_SITE 3

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE 12

[PROSITE] ASNJ3-LYCOSYLATION 1

[KW] All_Alpha

[KW] LOW_COMPLEXITY 20.13 %

SEQ MATGADVRDILELGGPEGDAASGTISKKDIINPDKKKSKKSSETLTFKRPEGMHREVYAL

SEG xxxxxxxxxxxxxxxxxx

PRD ccceeeeeeeeeeccccccccccccccccccccccccccccccccccccccchhhhhhhh

SEQ LYSDKKDAPPLLPSDTGQGYRTVKAKLGSKKVRPWKWMPFTNPARKDGAMFFHWRRAAEE SEG

PRD hhhhccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhc

SEQ GKDYPFARFNKTVQEPVYSEQEYQLYLHDNAWTKAETDHLFDLSRRFDLRFVVIHDRYDH SEG

PRD ccccccccccccccccchhhhhhhhhhhcchhhhhhhhhhhhhhhhccceeeeeeccccc

SEQ QQFKKRΞVEDLKERYYHICAKLANVRAVPGTDLKIPVFDAGHERRRKEQLERLYNRTPEQ SEG

PRD chhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccchhhhhhhhhhhhhhhhhcchhh

SEQ VAEEEYLLQELRKIEARKKEREKRSQDLQKLITAADTTAEQRRTERKAPKKKLPQKKEAE

SEG xxxxxxxxxxxxxx xxxxxxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhh

SEQ KPAVPETAGIKFPDFKSAGVTLRSQRMKLPSSVGQKKIKALEQMLLELGVELSPTPTEEL SEG xxx PRD hccccccccccccccccceeehhhhhhhccccccchhhhhhhhhhhhhhhhcccccchhh

SEQ VHMFNELRSDLVLLYELKQACANCEYELQMLRHRHEALARAGVLGGPATPASGPGPASAE

SEG xxxxxxxxxxxxxxxx

PRD hhhhhhccchhhhhhhhhhhcccchhhhhhhhhhhhhhhhhccccccccccccccccccc

SEQ PAVSEPGLGPDPKDTIIDVVGAPLTPNSRKRREΞASSSSSVKKAKKP

SEG xxxxxxx xxxxxxxxxxxxxxxxxxx .

PRD cccccccccccccceeeeeccccccccccccccccccccceeecccc

Prosite for DKFZphfbr2_64c4.2

PS00001 130->134 ASN_GLYCOSYLATION PDOC00001 PS00002 412->416 GLYCOSAMI OGLYCAN PDOC00002 PS00004 35->39 CAMP_PHOSPHO_SITE PDOC00004 PS00004 39->43 CAMP_PHOSPHO_ΞITE PDOC00004 PS00004 184->188 CAMP_PHOSPHO_SITE PDOC00004 PS00004 451->455 CAMP_PHOSPHO_SITE PDOC00004 PS00005 26->29 PKC_PHOSPHO_SITE PDOC00005 PS00005 38->41 PKC_PHOSPHO_SITE PDOC00005 PS00005 46->49 PKC_PHOSPHO_SITE PDOC00005 PS00005 63->66 PKC_PHOSPHO_SITE PDOC00005 PS00005 82->85 PKC_PHOSPHO_SITE PDOC00005 PS00005 89->92 PKC_PHOSPHO_SITE PDOC00005 PS00005 164->167 PKC_PHOSPHO_SITE PDOC00005 PS00005 284->287 PKC_PHOSPHO_SITE PDOC00005 PS00005 321->324 PKC_PHOSPHO_SITE PDOC00005 PS00005 324->327 PKC_PHOSPHO_SITE PDOC00005 PS00005 448->451 PKC_PHOSPHO_SITE PDOC00005 PS00005 460->463 PKC_PHOSPHO_SITE PDOC00005 PS00006 3->7 CK2_PHOSPHO_SITE PDOC00006 PS00006 26->30 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 132->136 CK2_PHOSPHO_SITE PDOC00006 PS00006 139->143 CK2_PHOSPHO_SITE PDOC00006 PS00006 153->157 CK2_PHOSPHO_SITE PDOC00006 PS00006 187->191 CK2_PHOSPHO_SITE PDOC00006 PS00006 273->277 CK2_PHOSPHO_SITE PDOC00006 PS00006 277->281 CK2_PHOSPHO_SITE PDOC00006 PS00006 355->359 CK2_PHOSPHO_SITE PDOC00006 PS00006 435->439 CK2_PHOSPHO_SITE PDOC00006 PS00007 131->139 TYR_PHOSPHO_SITE PDOC00007 PS00007 227->235 TYR_PHOSPHO_SITE PDOC00007 PS00007 116->125 TYR_PHOSPHO_SITE PDOC00007 PS00008 14->20 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_64c4.2)

DKFZphfbr2_64h6

group: brain derived

DKFZphfbr2_64h6 encodes a novel 176 ammo acid protein with similarity to predicted yeast proteins .

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application m studying the expression profile of brain-specific genes . similarity to S. pombe SPBC337.09 and S. cerevisiae YER044c complete cDNA, complete eds accoring to YER044c/SPBC337.09, start at Bp 111, EST hits

Sequenced by Qiagen

Locus: /map="14"

Insert length: 1212 bp

Poly A stretch at pos. 1192, polyadenylation signal at pos. 1168

1 GGGCTGGAGC TGTCCTGGGG GAGCTTGTTT GCGGCAGCGG CTGCTGCTGC 51 CACTGCTGTG CTGGGGGCCC GGTCGCCAGG CAAAAAGCCC TCCCACGTTT

101 GAGGGGAGTC ATGAGCCGTT TCCTGAATGT GTTAAGAAGT TGGCTGGTTA

151 TGGTGTCCAT CATAGCCATG GGGAACACGC TGCAGAGCTT CCGAGACCAC

201 ACTTTTCTCT ATGAAAAGCT CTACACTGGC AAGCCAAACC TTGTGAATGG

251 CCTCCAAGCT CGGACCTTTG GGATCTGGAC GCTGCTCTCA TCAGTGATCC

301 GCTGCCTCTG TGCCATTGAC ATTCACAACA AGACGCTCTA TCACATCACA

351 CTCTGGACCT TCCTCCTTGC CCTGGGGCAT TTCCTCTCTG AGTTGTTTGT

401 CTATGGAACT GCAGCTCCCA CGATTGGCGT CCTGGCACCC CTGATGGTGG

451 CAAGTTTCTC CATCCTGGGT ATGCTGGTCG GGCTCCGGTA TCTAGAAGTA

501 GAACCAGTAT CCAGACAGAA GAAGAGAAAC TGAGGCCAGC ATTATCACCT

551 CCAGGACTTT CTCGTTTTCC ACCTTGGCCA TCTTCTTCCT TCGTCGTCTC

601 TCCCCTTTAA TTTCTTTTCT ATTCCATCAT CTGCCCTTTT ACTCACTTTT

651 AGCCTCTTTT TTTAATTTTT AAAATTTAAA GATATGCATA CTGAAAAGTA

701 TATAACATGT ACGTACAATT TAAAGAATAA TTTTAAAGTG AATACTACGT

751 AACTCCATCC AAGTCAAGAA ATTGCCAGCT TCTCGGAAGC CCACTGTGTC

801 TCCTTCCCCT ACCTGCAACC TCTTCCAGGC TCCCTTTTCC AGCCTTCCCC

851 TTTTTCCCTT TTATTTTCAT GCCTTGATTT GACTTGTGTG GTGGGAACAT

901 GTGAACTATG AAACTTAAAC CTGCTGCCCA CCCAGAGCAG CTGTGACCAA

951 GGGCTGCCTC AAGGGGTTGT CCACGCAGGT TGGGCTCCTC TCTGCTGCTG 1001 GACCCAAGAC TCTGAACCTT CCAAGGGACA GGCAGTTCTT CTGAGAAGGG 1051 CTCCCCTGTG TGTGAGCAAG ACCACAGCTC TCCTTCTATC TACAGATGCA 1101 TGAGGGTTGG AAGAGTCTGG GCTGTTTTTA GACCTTCTGG TCAGCTGTAT 1151 TTGTGTAACA ACTTTTGTAA TAAATAGAAA AACCCTCTGC TCAAAAAAAA 1201 AAAAAAAAAA AA

BLAST Results

Entry G38566 from database EMBL:

SHGC-64295 Human Homo sapiens STS genomic, sequence tagged site.

Score = 1398, P = 1.4e-56, identities = 284/288

Medline entries

No Medlme entry

Peptide information for frame 3

ORF from 0 bp to 530 bp; peptide length: 177 Category: similarity to unknown protein Classification: unclassified

1 AGAVLGELVC GSGCCCHCCA GGPVARQKAL PRLRGVMSRF LNVLRSWLVM

51 VSIIAMGNTL QSFRDHTFLY EKLYTGKPNL VNGLQARTFG IWTLLSSVIR

101 CLCAIDIHNK TLYHITLWTF LLALGHFLSE LFVYGTAAPT IGVLAPLMVA 151 SFSILGMLVG LRYLEVEPVS RQKKRN

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_64h6, frame 3

TREMBL :SPBC337_9 gene: "SPBC337.09"; product: "conserved hypothetical protein"; S. pombe chromosome II cosmid c337., N = 1, Score = 224, P = 1.4e-18

PIR:S50547 hypothetical protein YER044c - yeast (Saccharomyces cerevisiae), N = 1, Score = 192, P = 3.4e-15

>TREMBL:SPBC337_9 gene: "SPBC337.09"; product: "conserved hypothetical protein"; S.pombe chromosome II cosmid c337. Length = 136

HSPs:

Score = 224 (33.6 bits), Expect = 1.4e-18, P = 1.4e-18 Identities = 49/113 (43%), Positives = 74/113 (65%)

Query: 42 NVLRSWLVMVSIIAMGNTLQSFRDHTFLYEKLYTGKPNLVNGLQARTFGIWTLLSΞVIRC 101

+++ W V+VS+ A+ NT+QSF L +++Y+ N VNGLQ RTFGIWTLLS+++R Sbjct: 11 SLVAKWNVVVSVAALFNTVQSFLTPK-LTKRVYSNT-NEVNGLQGRTFGIWTLLSAIVRF 68

Query: 102 LCAIDIHNKTLYHITLWTFLLALGHFLSELFVYGTAAPTIGVLAPLMVASFSI 154

CA I N +Y + T+ LA HFLSE ++ T G+L+P++V++ SI Sbjct: 69 YCAYHITNPDVYFLCQCTYYLACFHFLSEWLLFRTTNLGPGLLSPIVVSTVSI 121

Pedant information for DKFZphfbr2_64h6, frame 3

Report for DKFZphfbr2_64h6.3

[LENGTH] 176 [MW] 19359.31 [pi] 9.53 [HOMOL] TREMBL :SPBC337_9 gene: "SPBC337.09"; product: "conserved hypothetical protein"

S. pombe chromosome II cosmid c337. 2e-17 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YER044c] 7e-16 [KW] TRANSMEMBRANE 2

[KW] LOW COMPLEXITY 7.39 ?

SEQ AGAVLGELVCGSGCCCHCCAGGPVARQKALPRLRGVMSRFLNVLRSWLVMVSI IAMGNTL SEG xxxxxxxxxxxxx PRD ccceeeeeeeeccceeeeccccccccccccccccchhhhhhhhhhhhhhheeeecccccc MEM MMMMMMMMMMMMMMMMM ....

SEQ QSFRDHTFLYEKLYTGKPNLVNGLQARTFGIWTLLSSVIRCLCAIDIHNKTLYHITLWTF SEG PRD ccccchhhhhhhhhhcccccccccccccccchhhhhhhhhhhhhhhccccceeeehhhhh

MEM

SEQ LLALGHFLSELFVYGTAAPTIGVLAPLMVASFSILGMLVGLRYLEVEPVSRQKKRN SEG PRD hhhhhhhhhhhhhhhccccccccccceeehhhhhhhhhhhheeeeecccccccccc MEM MMMMMMMMMMMMMMMMM

(No Prosite data available for DKFZphfbr2_64h6.3) (No Pfam data available for DKFZphfbr2_64h6.3) DKFZphfbr2_64jl8

group: Intracellular transport and trafficking

DKFZphfbr2_624jl8.1 encodes a novel 180 amino acid protein nearly identical to the microsomal signal peptidase 23 kd subunit of cams familiaris, gallus gallus and C. elegans.

The new protein is identical to canine and chicken microsomal signal peptidase 23 kd subunit. The canine microsomal signal peptidase is a protein complex comprised of five subunits (25, 22/23, 21, 18, and 12 kDa) . The 23kDa subunit is tightly associated with the 18- and 21-kDa subunits, that are integral membrane proteins.

The new protein can find application in modulation of protein transport into microsomal compartments and as a tool for proteomic analysis. strong similarity to dog signal peptidase (EC 3.4.99.-) complete cDNA, complete eds, potential start at Bp 109, EST hits,

Sequenced by Qiagen

Locus : unknown

Insert length: 690 bp

Poly A stretch at pos. 666, polyadenylation signal at pos. 646

1 GCCGGAACGC GCGCACCGCA GACGGCGCGG ATCGCAGGGA GCCGGTCCGC

51 CGCCGGAACG GGAGCCTGGG TGTGCGTGTG GAGTCCGGAC TCGTGGGAGA

101 CGATCGCGAT GAACACGGTG CTGTCGCGGG CGAACTCACT GTTCGCCTTC

151 TCGCTGAGCG TGATGGCGGC GCTCACCTTC GGCTGCTTCA TCACCACCGC

201 CTTCAAAGAC AGGAGCGTCC CGGTGCGGCT GCACGTCTCG CGGATCATGC

251 TAAAAAATGT AGAAGATTTC ACTGGACCTA GAGAAAGAAG TGATCTGGGA

301 TTTATCACAT CTGATATAAC TGCTGATCTA GAGAATATAT TTGATTGGAA

351 TGTTAAGCAG TTGTTTCTTT ATTTATCAGC AGAATATTCA ACAAAAAATA

401 ATGCTCTGAA CCAAGTTGTC CTATGGGACA AGATTGTTTT GAGAGGTGAT

451 AATCCGAAGC TGCTGCTGAA AGATATGAAA ACAAAATATT TTTTCTTTGA

501 CGATGGAAAT GGTCTCAAGG GAAACAGGAA TGTCACTTTG ACCCTGTCTT

551 GGAACGTCGT ACCAAATGCT GGAATTCTAC CTCTTGTGAC AGGATCAGGA

601 CACGTATCTG TCCCATTTCC AGATACATAT GAAATAACGA AGAGTTATTA

651 AATTATTCTG AATTTGAAAC AAAAAAAAAA AAAAAAAAAA

BLAST Results

No BLAST result

Medline entries

89034208: cDNA-deπved primary structure of the glycoprotein component of canine microsomal signal peptidase complex.

Peptide information for frame 1

ORF from 109 bp to 648 bp; peptide length: 180 Category: strong similarity to known protein Prosite motifs: TONB_DEPENDENT_REC_l (1-58) RGD (148-151)

1 MNTVLSRANS LFAFSLSVMA ALTFGCFITT AFKDRSVPVR LHVSRIMLKN

51 VEDFTGPRER SDLGFITSDI TADLENIFDW NVKQLFLYLS AEYSTKNNAL

101 NQVVLWDKIV LRGDNPKLLL KDMKTKYFFF DDGNGLKGNR NVTLTLSWNV

151 VPNAGILPLV TGSGHVSVPF PDTYEITKSY

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_64j 18, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_64j 18, frame 1

Report for DKFZphfbr2_64j 18.1

[LENGTH] 180

[MW] 20253.39

[pi] 8.66

[HOMOL] PIR:A31788 signal peptidase (EC 3.4.99.-) (SPC 22/23) - dog le-100

[FUNCAT] 30.07 organization of endoplasmatic reticulum [S. cerevisiae, YLR066w]

6e-15

[FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YLR066w] 6e-15

[PIRKW] transmembrane protein 2e-92

[PIRKW] glycoprotein 2e-92

[PIRKW] hydrolase 2e-92

[PROSITE] RGD 1

[PROSITE] MYRISTYL 2

[PROSITE] PROKAR_LIPOPROTEIN 1

[PROSITE] TONB_DEPENDENT_REC_l 1

[PROSITE] PKC_PHOSPHO_SITE 1

[PROSITE] ASN_GLYCOSYLATION 1

[KW] Alpha_Beta

[KW] SIGNAL PEPTIDE 32

SEQ. MNTVLSRANSLFAFSLSVMAALTFGCFITTAFKDRSVPVRLHVSRIMLKNVEDFTGPRER PRD ccccccchhhhhhhhhhhhhhhhhhhhhheeecccccceeehhhhhhhhhhhhccccccc

SEQ SDLGFITSDITADLENIFDWNVKQLFLYLSAEYSTKNNALNQVVLWDKIVLRGDNPKLLL PRD ccccchhhhhhhhccccccchhhhhhhhhhhhhhhccccceeeeeeeceeecccchhhhh

SEQ KDMKTKYFFFDDGNGLKGNRNVTLTLSWNVVPNAGILPLVTGSGHVSVPFPDTYEITKSY PRD hhcccceeeeecccccccccceeeeeeeecccccceeeeeccccceeeeccccccccccc

Prosite for DKFZphfbr2_64 j 18.1

PS00001 141->145 ASN_GLYCOSYLATION PDOC00001

PS00005 94->97 PKC_PHOSPHO_SITE PDOC00005

PS00008 25->31 MYRISTYL PDOC00008

PS00008 135->141 MYRISTYL PDOC00008

PS00013 16->27 PROKAR_LIPOPROTEIN PDOC00013

PS00016 112-M15 RGD PDOC00016

PS00430 l->22 TONB DEPENDENT REC 1 PDOC00354

(No Pfam data available for DKFZphfbr2_64 l8.1)

DKFZphfbr2_64k24

group: transmembrane proteins

DKFZphfbr2_64k24 encodes a novel 412 am o acid protein with weak similarity to several known proteins .

The novel protein contains 5 transmembrane regions.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes and as a new marker for neuronal cells. similarity to AMAC1 "testicular condensing enzyme" ; membrane regions: 5

Summary DKFZphfbr2_64k24 encodes a novel 412 ammo acid protein, with similarity to AMAC1"; product: "testicular condensing enzyme similarity to AMAC1 "testicular condensing enzyme" complete cDNA, complete eds, EST hits

Sequenced by Qiagen

Locus : unknown

Insert length: 1958 bp

Poly A stretch at pos. 1939, polyadenylation signal at pos. 1918

1 GGGCCCGCCT CGATTTTCCC AGGCGAGGGC ACGCCCGCGT CAGTCGCCTC 51 CGGGGCACCT TCCTCGCCAC GACACGCAGG TAACCGGGCC CCGGGAGCCG

101 GTCGGCGGCG GCGGACTGGG ACCTTGATCC TGCCTGCCCG GCCGCCCGAC

151 AAGGGAATGA GAGCGGACCC CGAACTCCAC ACACCCGCGT TTAGCCGCCA

201 CACCTAAGGG GCAGAACAGT CTTTTTGGGT AAGGGCCGGG CTGGGGGCGA

251 CGCGCCCCGC CCGCTTTGCA GACTTCGGGG TGCTCTGCAC GACGCCTGAA

301 AGGCCGCGGG GCCCGCATTT CTCTGTGCTG CCCTCCTGGA GAACCGGGAC

351 ACGGGGACGG GAGGGCCAGC ATCGGCTACG GCCCGGTTTC CCGTTTCTTT

401 CCTCTGTCGC GTCTGGGCCC TCCTGCAGCG TCCATGATGA AGGCCAGGGG

451 CTGTTGCTTT CCTCTCGCCC AGTAGCCAAC CCAAGCAAGG GAATTAATTA

501 TCTGAAGAAA TGGATACTTC TCCCTCCAGA AAATATCCAG TTAAAAAACG

551 GGTGAAAATA CATCCCAACA CAGTGATGGT GAAATATACT TCTCATTATC

601 CCCAGCCTGG CGATGATGGA TATGAAGAAA TCAATGAAGG CTATGGGAAT

651 TTTATGGAGG AAAATCCAAA GAAAGGTCTG CTGAGTGAAA TGAAAAAAAA

701 AGGGAGAGCT TTCTTTGGAA CCATGGATAC CCTACCTCCA CCAACAGAAG

751 ACCCAATGAT CAATGAGATT GGACAATTCC AGAGCTTTGC AGAAAAAAAC

801 ATTTTTCAAT CCCGAAAAAT GTGGATAGTG CTGTTTGGAT CTGCTTTGGC

851 TCATGGATGT GTAGCTCTTA TCACTAGGCT TGTTTCTGAT CGGTCTAAAG

901 TTCCATCTCT AGAACTGATT TTTATCCGTT CTGTTTTTCA GGTCTTATCT

951 GTGTTAGTTG TGTGTTACTA TCAGGAGGCC CCCTTTGGAC CCAGTGGATA 1001 CAGATTACGA CTCTTCTTTT ATGGTGTATG CAATGTCATT TCTATCACTT 1051 GTGCTTATAC ATCATTTTCA ATAGTTCCTC CCAGCAATGG GACCACTATG 1101 TGGAGAGCCA CAACTACAGT CTTCAGTGCC ATTTTGGCTT TTTTACTCGT 1151 AGATGAGAAA ATGGCTTATG TTGACATGGC TACAGTTGTT TGCAGCATCT 1201 TAGGTGTTTG TCTTGTCATG ATCCCAAACA TTGTTGATGA AGACAATTCT 1251 TTGTTAAATG CCTGGAAAGA AGCCTTTGGG TACACCATGA CTGTGATGGC 1301 TGGACTGACC ACTGCTCTCT CAATGATAGT ATACAGATCC ATCAAGGAGA 1351 AGATCAGCAT GTGGACTGCG CTGTTTACTT TTGGTTGGAC TGGGACAATT 1401 TGGGGAATAT CTACTATGTT TATTCTTCAA GAACCCATCA TCCCATTAGA 1451 TGGAGAAACC TGGAGTTATC TCATTGCTAT ATGTGTCTGT TCTACTGCAG 1501 CATTCTTAGG AGTTTATTAT GCCTTGGACA AATTCCATCC AGCTTTGGTT 1551 AGCACAGTAC AACATTTGGA GATTGTGGTA GCTATGGTCT TGCAGCTTCT 1601 CGTGCTGCAC ATATTTCCTA GCATCTATGA TGTTTTTGGA GGGGTAATCA 1651 TTATGATTAG TGTTTTTGTC CTTGCTGGCT ATAAACTTTA CTGGAGGAAT 1701 TTAAGAAGGC AGGACTACCA GGAAATACTA GACTCTCCCA TTAAATGAAT 1751 ACCTGATTAT TATTGTCTCA TTAATGTTCA GTTATTAATA TGTATACTGC 1801 CATTTTAATG TTTACCTATG AATGTCTTTT GTGTTATATA ACTGACAGAG 1851 TGCTATAAAA TATATAATAT ATACAAATGC AGAAAATTTA TTCTAGTCTA 1901 ATATATTCAA ATACAAATAT TAAATATATG AAATACGTTA AAAAAAAAAA 1951 AAAAAAAA

BLAST Results o BLAST result Medline entries

No Medline entry

Peptide information for frame 3

ORF from 510 bp to 1745 bp; peptide length: 412 Category: similarity to known protein

1 MDTSPSRKYP VKKRVKIHPN TVMVKYTSHY PQPGDDGYEE INEGYGNFME

51 ENPKKGLLSE MKKKGRAFFG TMDTLPPPTE DPMINEIGQF QSFAEKNIFQ

101 SRKMWIVLFG SALAHGCVAL ITRLVSDRSK VPSLELIFIR SVFQVLSVLV

151 VCYYQEAPFG PSGYRLRLFF YGVCNVISIT CAYTSFΞIVP PSNGTTMWRA

201 TTTVFSAILA FLLVDEKMAY VDMATVVCSI LGVCLVMIPN IVDEDNSLLN

251 AWKEAFGYTM TVMAGLTTAL SMIVYRSIKE KISMWTALFT FGWTGTIWGI

301 STMFILQEPI IPLDGETWSY LIAICVCSTA AFLGVYYALD KFHPALVSTV

351 QHLEIVVAMV LQLLVLHIFP SIYDVFGGVI IMISVFVLAG YKLYWRNLRR 401 QDYQEILDSP IK

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_64k24, frame 3

TREMBLNEW:AF016712_1 gene: "AMACl"; product: "testicular condensing enzyme"; Mus musculus testicular condensing enzyme (AMACl) mRNA, complete eds., N = 1, Score = 191, P = 1.9e-12

TREMBL :BMAJ733_6 product: "hypothetical protein"; Bacillus megaterium bgaM gene, N = 1, Score = 137, P = 1.6e-06

PIR:G71841 hypothetical protein jhpll55 - Helicobacter pylori (strain J99), N = 1, Score = 129, P = 1.3e-05

>TREMBLNEW:AF016712_1 gene: "AMACl"; product: "testicular condensing enzyme"; Mus musculus testicular condensing enzyme (AMACl) mRNA, complete eds .

Length = 362

HSPs:

Score = 191 (28.7 bits), Expect = 1.9e-12, P = 1.9e-12 Identities = 39/105 (37%), Positives = 66/105 (62%)

Query: 289 FTFGWTGTIWGISTMFILQEPIIPLDGETWSYLIAICVCSTAAFLGVYYALDKFHPALVS 348

F FG G + + +F+LQ P++P D +WS ++A+ + + +F+ V YA+ K HPALV Sbjct: 248 FLFGLVGLMVSVPGLFVLQTPVLPQDTLSWSCVVAVGLLALVSFVCVSYAVTKAHPALVC 307

Query: 349 TVQHLEIVVAMVLQLLVLH—IFPSIYDVFGGVIIMISVFVLAGYKL 393

V H E+VVA++LQ VL+ + PS D+ G +++ Ξ+ ++ L Sbjct: 308 AVLHSEVVVALMLQYYVLYETVAPS--DIMGAGVVLGSIAIITAQNL 352

Pedant information for DKFZphfbr2_64k24, frame 3

Report for DKFZphfbr2_64k24.3

[LENGTH] 412

[MW] 46449.87

[pi] 6.99

[HOMOL] TREMBL:AF016712_1 gene: "AMACl"; product: "testicular condensing enzyme"; Mus musculus testicular condensing enzyme (AMACl) mRNA, complete eds. 8e-14

[PROSITE] MYRISTYL 6

[PROSITE] CK2_PHOSPHO_SITE 3

[PROSITE] PKC_PHOSPHO_SITE 4

[PROSITE] ASN_GLYCOSYLATION 1

[KW] TRANSMEMBRANE 5

SEQ MDTSPSRKYPVKKRVKIHPNTVMVKYTSHYPQPGDDGYEEINEGYGNFMEENPKKGLLSE PRD ccccccccccccceeeecccceeeeeecccccccccceeeeecccccccccccccchhhh

MEM

SEQ MKKKGRAFFGTMDTLPPPTEDPMINEIGQFQSFAEKNIFQSRKMWIVLFGSALAHGCVAL

PRD hhhhcceeecccccccccccccceeeecccchhhhhhhhccceeeeeeeccccchhhhhc

MEM

SEQ ITRLVSDRSKVPSLELIFIRSVFQVLSVLVVCYYQEAPFGPSGYRLRLFFYGVCNVISIT

PRD chhhhhccccccccchhhhhhhhhhhheeeeeeeccccccccceeeeeeeecceeeeeee

MEM MMMMMMMMMMMMMMMMM

SEQ CAYTSFSIVPPSNGTTMWRATTTVFΞAILAFLLVDEKMAYVDMATVVCSILGVCLVMIPN

PRD eccceeeeccccccceeeeeehhhhhhhhhhhhhhhhheeeeeeeeeeeeeeeeeeeecc

MEM

SEQ IVDEDNSLLNAWKEAFGYTMTVMAGLTTALSMIVYRSIKEKISMWTALFTFGWTGTIWGI

PRD cccccchhhhhhhhhhhheeeeeeehhhhhhhcchhhhhhhhhhhhccccccccceeeec

MEM MMMMMMMMMMMMMMMMMMM

SEQ STMFILQEPIIPLDGETWSYLIAICVCSTAAFLGVYYALDKFHPALVSTVQHLEIVVAMV

PRD ceeeeeecccccccccceeeeeccchhhhhhhhhccccccccccchhhhhhhhhhhhhhh

MEM MMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMM

SEQ LQLLVLHIFPSIYDVFGGVIIMISVFVLAGYKLYWRNLRRQDYQEILDSPIK

PRD hhhhhhhhhccccccceeeeeeeeeecccccchhhhhhhhhhhhhhhccccc

MEM MMMMMMM....MMMMMMMMMMMMMMMMMMMMM

Prosite for DKFZphfbr2_64k24.3

PS00001 193->197 ASN_GLYCOSYLATION PDOC00001 PS00005 6->9 PKC_PHOSPHO_SITE PDOC00005 PS00005 101->104 PKC_PHOSPHO_SITE PDOC00005 PS00005 126->129 PKC_PHOSPHO_SITE PDOC00005 PS00005 277->280 PKC_PHOSPHO_ΞITE PDOC00005 PS00006 92->96 CK2_PHOSPHO_SITE PDOC00006 PS00006 277->281 CK2_PHOSPHO_SITE PDOC00006 PS00006 371->375 CK2_PHOSPHO_SITE PDOC00006 PS00008 70->76 MYRISTYL PDOC00008 PS00008 88->94 MYRISTYL PDOC00008 PS00008 110->116 MYRISTYL PDOC00008 PS00008 265->271 MYRISTYL PDOC00008 PS00008 295->301 MYRISTYL PDOC00008 PS00008 334->340 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_64k24.3)

DKFZphfbr2 6al7

group: brain derived

DKFZphfbr2_6al7 encodes a novel 100 amino acid protein with very weak similarity to human finger protein zfOCl.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . complete cDNA, complete eds, EST hits

Sequenced by AGOWA

Locus : unknown

Insert length: 1424 bp

Poly A stretch at pos. 1405, polyadenylation signal at pos. 1389

1 GGGACTGAGG GGGTGGGCTT ACTCCCTGGG CAGTCTTGGG GGCCAGAGCT 51 GAGGCCAGTC CATATTACAG TGGCTGGGCT GTTTTTTTCA GTAGCCCCTA

101 GCATTGGCTG GGATTCCTGT TCCTGGGTGC GCCTCCACCT CCCTTCTGAT

151 GCTTCCTGGC TATGGTGGGG TGGGAACCTC AGTTTCCCCC AAAGTCTTCC

201 CTGGATGCTG GCTTCAGGTT GAAGACCCTG GTTCTTCCAG TTCCTCACGG

251 GTTAGGTAGG GGCTCCTGCA TCACCTTCAG AATCAGTTCC AACCCCCACT

301 CTCCTTAGGC TTTGTGCTCT GCTCTGCCCT GCCAGGCTGC CCTTGTCCAT

351 GTGAGTAGCA TGGGCGGGTG GTGGGGACGG CAGTGGTGAT GAAGGGGGTG

401 CACCACAGGC CTCATGAAGC AGTTCCCACA TGGGCGTGTG GCTGGGGCGT

451 GGCCACCACA GAGCACATGG CTGTGTCTAG GCGCAAGCAC TTTAGCAGTA

501 TCTGTTTACA TGCGCAAGGA TCAAGCCGAC TACCTGTGCT GTCTACTGGG

551 ACAGCAGTCT CCGAGCTACT CCGTACCTCC CTCTGCCAGG TCGTGGAGTT

601 AGGCCCCAGT CCCTACTTGT CACTGGTTCC CACTGTGCTC CTAACTGTGC

651 AGCACCTGGG AGCTCTGGCC TGGGGCTGGA GGCCCTGGTA GGAGCTGCAG

701 TTGGAGGCCG TTCTGTGCCC AGCAGCGGTG AGCGGCTCCC ATGGGCCCTG

751 TGTCTGCAGG GAGCCAGGGC TGCGGCACAT GTGCTGTGAA ACTGGCACCC

801 ACCTGGCGTG CTGCTGCCGC CACTTGCTTC CTGCAGCACC TCCTACCCTG

851 CTCCGTGTCC TCCCTCTCCC CGCGCCTGGC TCAGGAGTGC TGGAAAAGCT

901 CACGCCTCGG CCTGGGAGCC TGGCCTCTTG ATATACCTCG AGCTTCCCCT

951 GTGCTCCCCA GCCCCAGGAC CACTGGCCCC TTGGCCTGAG GGGCTGGGGG 1001 CCCCACGACC TGCAGCGTCG AGTCCGGGAG AGAGCCCGGA GCGGCGTGCC 1051 ATCTCGGCTC GGCCTTGCTG AGAGCCTCCG CCCTGGCTTT CTCCCTGTCT 1101 GGTTTCAGTG GCTCACGTTG GTGCTACACA GCTAGAATAG ATATATTTAG 1151 AGAGAGAGAT ATTTTTAAGA CAAAGCCCAC AATTAGCTGT CCTTTAACAC 1201 CGCAGAACCC CCTCCCAGAA GAAGAGCGAT CCCTCGGACG GTCCGGGCGG 1251 GCACCCTCAG CCGGGCTCTT TGCAGAAGCA GCACCGCTGA CTGTGGGCCC 1301 GGCCCTCAGA TGTGTACATA TACGGCTATT TCCTATTTTA CTGTTCTTCA 1351 GATTTAGTAC TTGTAAATAA ACACACACAT TAAGGAGAGA TTAAACATTT 1401 TTGCCAAAAA AAAAAAAAAA AAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 389 bp to 688 bp; peptide length: 100 Category: putative protein

1 MKGVHHRPHE AVPTWACGWG VATTEHMAVS RRKHFSSICL HAQGΞSRLPV 51 LSTGTAVSEL LRTSLCQVVE LGPSPYLSLV PTVLLTVQHL GALAWGWRPW

BLASTP hits Entry S70007 from database PIR : fmger protein zfOCl - human ( fragment )

Length = 183

Score = 62 (21.8 bits), Expect = 0.24, Sum P(2) = 0.22

Identities = 18/47 (38%), Positives = 24/47 (51%)

Alert BLASTP hits for DKFZphfbr2_6al7, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_6al7, frame 2

Report for DKFZphfbr2_6al7.2

[LENGTH] 100

[MW] 10944.82

[pi] 9.49

[PROSITE] MYRISTYL 2

[PROSITE] PKC_PHOSPHO_SITE

[KW] Alpha Beta

SEQ MKGVHHRPHEAVPTWACGWGVATTEHMAVSRRKHFSSICLHAQGSSRLPVLSTGTAVSEL

PRD cccccccccccccccccccccchhhhhhhhhhcccccceeeccccccceeecccchhhhh

SEQ LRTΞLCQVVELGPSPYLSLVPTVLLTVQHLGALAWGWRPW

PRD hhhhheeeeecccccceeecchhhhhhhhhchhhhhcccc

Prosite for DKFZphfbr2_6al7.2

PS00005 30->33 PKC_PHOSPHO_SITE PDOC00005 PS00005 45->48 PKC_PHOSPHO_SITE PDOC00005 PS00008 20->26 MYRISTYL PDOC00008 PS00008 54->60 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_6al7.2)

DKFZphfbr2_6b24

group: metabolism

DKFZphfkd2 6b24 encodes a novel 334 amino acid protein with similarity to several bacterial dTDP-4-dehydrorhamnose reductases (EC 1.1.1.133).

The novel protein seems to be a human enzyme similar to dTDP-4-dehydrorhamnose reductases. EC 1.1.1.133 catalises the reaction: dTDP-6-deoxy-L-mannose + NADP(+) <=> dTDP-4-dehydro-6-deoxy- L-mannose + NADPH.

The new protein can find application in modulation of rhamnose metabolism and as a new enzyme for biotechnologic production processes. similar to dTDP-6-deoxy-L-mannose-dehydrogenases complete cDNA, EST hits, complete eds

Nucleotide sugars metabolism seems to be a dehydrogenase localisation: region of primer A missing

Sequenced by AGOWA

Locus: /map="5"

Insert length: 2054 bp

Poly A stretch at pos. 2028, polyadenylation signal at pos. 2015

1 GGGGGAGGCC CGCGTCGATC CTGGGTTGGA GGAGGTGGCG GCCGCTGAGG

51 CTGCGGCGTG AAGACGGCGG GCATGGTGGG GCGGGAGAAA GAGCTCTCTA

101 TACACTTTGT TCCCGGGAGC TGTCGGCTGG TGGAGGAGGA AGTTAACATC

151 CCTAATAGGA GGGTTCTGGT TACTGGTGCC ACTGGGCTTC TTGGCAGAGC

201 TGTACACAAA GAATTTCAGC AGAATAATTG GCATGCAGTT GGCTGTGGTT

251 TCAGAAGAGC AAGACCAAAA TTTGAACAGG TTAATCTGTT GGATTCTAAT

301 GCAGTTCATC ACATCATTCA TGATTTTCAG CCCCATGTTA TAGTACATTG

351 TGCAGCAGAG AGAAGACCAG ATGTTGTAGA AAATCAGCCA GATGCTGCCT

401 CTCAACTTAA TGTGGATGCT TCTGGGAATT TAGCAAAGGA AGCAGCTGCT

451 GTTGGAGCAT TTCTCATCTA CATTAGCTCA GATTATGTAT TTGATGGAAC

501 AAATCCACCT TACAGAGAGG AAGACATACC AGCTCCCCTA AATTTGTATG

551 GCAAAACAAA ATTAGATGGA GAAAAGGCTG TCCTGGAGAA CAATCTAGGA

601 GCTGCTGTTT TGAGGATTCC TATTCTGTAT GGGGAAGTTG AAAAGCTCGA

651 AGAAAGTGCA GTGACTGTTA TGTTTGATAA AGTGCAGTTC AGCAACAAGT

701 CAGCAAACAT GGATCACTGG CAGCAGAGGT TCCCCACACA TGTCAAAGAT

751 GTGGCCACTG TGTGCCGGCA GCTAGCAGAG AAGAGAATGC TGGATCCATC

801 AATTAAGGGA ACCTTTCACT GGTCTGGCAA TGAACAGATG ACTAAGTATG

851 AAATGGCATG TGCAATTGCA GATGCCTTCA ACCTCCCCAG CAGTCACTTA

901 AGACCTATTA CTGACAGCCC TGTCCTAGGA GCACAACGTC CGAGAAATGC

951 TCAGCTTGAC TGCTCCAAAT TGGAGACCTT GGGCATTGGC CAACGAACAC

1001 CATTTCGAAT TGGAATCAAA GAATCACTTT GGCCTTTCCT CATTGACAAG

1051 AGATGGAGAC AAACGGTCTT TCATTAGTTT ATTTGTGTTG GGTTCTTTTT

1101 TTTTTTAAAT GAAAAGTATA GTATGTGGCC CTTTTTAAAG AACAAAGGAA

1151 ATAGTTTTGT ATGAGTACTT TAATTGTGAC TCTTAGGATC TTTCAGGTAA

1201 ATGATGCTCT TGCACTAGTG AAATTGTCTA AAGAAACTAA AGGGCAGTCA

1251 TGCCCTGTTT GCAGTAATTT TTCTTTTTAT CATTATGTTT GTCCTGGCTA

1301 AACTTGGAGT TTGAGTATAG TAAATTATGA TCCTTAAATA TTTGAGGGTC

1351 AGGATGAAGC AGATCTGCTG TAGACTTTTC AGATGAAATT GTTCATTCTC

1401 GTAACCTCCA TATTTTCAGG ATTTTTGAAG CTGTTGACCA TTTCATGTTG

1451 ATTATTTTAA ATTGTGTGGA ATAGTATAAA AATCATTGGT GTTCATTATT

1501 TGCTTTGCCT GAGCTCAGAT CAAAATGTTT GAAGAAAGGA ACTTTATTTT

1551 TGCAAGTTAC GTACAGTTTT TATGCTTGAG ATATTTCAAC ATGTTATGTA

1601 TATTGGAACT TCTACAGCTT GATGCCTCCT GCTTTTATAG CAGTTTATGG

1651 GGAGCACTTG AAAGAGCGTG TGTACATGTA TTTTTTTTCT AGGCAAACAT

1701 TGAATGCAAA CGTGTATTTT TTTAATATAA ATATATAACT GTCCTTTTCA

1751 TCCCATGTTG CCGCTAAGTG ATATTTCATA TGTGTGGTTA TACTCATAAT

1801 AATGGGCCTT GTAAGTCTTT TCACCATTCA TGAATAATAA TAAATATGTA

1851 CTGCTGGCAT GTAATGCTTA GTTTTCTTGT ATTTACTTCT TTTTTTTAAA

1901 TGTAAGGACC AAACTTCTAA ACTAATTGTT CTTTTGTTGC TTTAATTTTT

1951 AAAAATTACA TTCTTCTGAT GTAACATGTG ATACATACAA AAGAATATAG

2001 TTTAATATGT ATTGAAATAA AACACAATAA AATTAAAAAA AAAAAAAAAA

2051 AAAA

BLAST Results

Entry G37115 from database EMBL: SHGC-56899 Human Homo sapiens STS genomic. Score = 446, P = 4.6e-14, identities = 90/91 Medline entries

99109950:

The metabolism of 6-deoxyhexoses in bacterial and animal cells .

Peptide information for frame 1

ORF from 73 bp to 1074 bp; peptide length: 334 Category: similarity to known protein

1 MVGREKELSI HFVPGSCRLV EEEVNIPNRR VLVTGATGLL GRAVHKEFQQ

51 NNWHAVGCGF RRARPKFEQV NLLDSNAVHH IIHDFQPHVI VHCAAERRPD

101 VVENQPDAAS QLNVDASGNL AKEAAAVGAF LIYISSDYVF DGTNPPYREE

151 DIPAPLNLYG KTKLDGEKAV LENNLGAAVL RIPILYGEVE KLEESAVTVM

201 FDKVQFSNKS ANMDHWQQRF PTHVKDVATV CRQLAEKRML DPSIKGTFHW

251 SGNEQMTKYE MACAIADAFN LPSSHLRPIT DSPVLGAQRP RNAQLDCSKL

301 ETLGIGQRTP FRIGIKESLW PFLIDKRWRQ TVFH

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_6b24, frame 1

PIR:T00104 probable dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133) - Actinobacillus actinomycetemcomitans, N = 1, Score = 293, P = 6.4e-26

TREMBL :SSU51197_21 gene: "rhsD"; product:

"dTDP-6-deoxy-L-mannose-dehydrogenase"; Sphingomonas S88 sphingan polysaccharide synthesis (spsG) , (spsS), (spsR) , glycosyl transferase (spsQ) , (spsl), glycosyl transferase (spsK), glycosyl transferase (spsL), (sps ), (spsF), (spsD), (spsC), (spsE), Urf 32, Urf 26,

ATP-bindmg cassette trans>., N = 1, Score = 291, P = le-25

SWISSPROT :RFBD_RHISN PROBABLE DTDP-4-DEHYDRORHAMNOSE REDUCTASE (EC 1.1.1.133) (DTDP-4-KETO- L-RHAMNOSE REDUCTASE) (DTDP-6-DEOXY-L-MANNOSE DEHYDROGENASE) (DTDP-L- RHAMNOSE SYNTHETASE) . , N = 1, Score = 283, P = 7.4e-25

>PIR:T00104 probable dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133) - Actinobacillus actinomycetemcomitans Length = 294

HSPs:

Score = 293 (44.0 bits), Expect = 6.4e-26, P = 6.4e-26 Identities = 89/276 (32%), Positives = 151/276 (54%)

Query: 30 RVLVTGATGLLGRAVHKEFQQNNWHAVGCGFRRARPKFEQVNLLDSNAVHHIIHDFQPHV 89

R+L+TGA G LGR++ K N + V F ++++ + + V II F+P+V

Sbjct: 3 RLLITGAGGQLGRSLAKLLVDNGRYEV LALDFSELDITNKDMVFSIIDSFKPNV 56

Query: 90 IVHCAAERRPDVVENQPDAASQLNVDASGNLAKEAAAVGAFLIYIΞSDYVFDG-TNPPYR 148

I++ AA D E + +A +NV LA+ A + ++++S+DYVFDG + Y+ Sbjct: 57 IINAAAYTSVDQAELEVSSAYSVNVRGVQYLAEAAIRHNSAILHVSTDYVFDGYKSGKYK 116

Query: 149 EEDIPAPLNLYGKTKLDGEKAVLENNLGAAVLRIPILYGEVEKLEESAVTVMFDKVQFSN 208

E Dl PL +YGK+K +GE+ +L + + +LR +GE + V M ++ + Sbjct: 117 ETDIIHPLCVYGKΞKAEGERLLLTLSPKSIILRTΞWTFGEYGN NFVKTML-RLAKNR 172

Query: 209 KSANMDHWQQRFPTHVKDVATVCRQLAEKRMLDPSIK-GTFHWSGNEQMTKYEMACAIAD 267

+ Q PT+ D+A+V Q+AEK ++ ++K G +H++G ++ Y+ A Al D Sbjct: 173 DILGVVADQIGGPTYSGDIASVLIQIAEKIIVGETVKYGIYHFTGEPCVSWYDFAIAIFD 232

Query: 268 AF NLPSSHLRPITDSPVLGAQRPRNAQLDCSKLE-TLGI 305

N+P + D P L A+RP N+ LD +K++ GI Sbjct: 233 EAVAQKVLENVPLVNAITTADYPTL-AKRPANSCLDLTKIQQAFGI 277 Pedant information for DKFZphfbr2_6b24, frame 1

Report for DKFZphfbr2_6b24.1

[LENGTH] 334

[MW] 37551.98

[pi] 6.90

[HOMOL] PIR:T00104 probable dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133) -

Actinobacillus actinomycetemcomitans 6e-25

[FUNCAT] 01.06.01 lipid, fatty-acid and sterol biosynthesis [S. cerevisiae, YGLOOlc]

6e-04

[EC] 1.1.1.133 dTDP-4-dehydrorhamnose reductase 2e-16

[PIRKW] lipopolysacchaπde biosynthesis 2e-16

[PIRKW] NADP 2e-16

[PIRKW] oxidoreductase 2e-16

[PIRKW] streptomycin biosynthesis le-19

[SUPFAM] dTDP-dihydrostreptose synthase le-20

[PROSITE] MYRISTYL 1

[PROSITE] CK2_PHOSPHO_SITE 4

[PROSITE] PKC_PHOSPHO_SITE 3

[PROSITE] ASN_GLYCOSYLATION 1

[KW] Alpha_Beta

SEQ MVGREKELSIHFVPGSCRLVEEEVNIPNRRVLVTGATGLLGRAVHKEFQQNNWHAVGCGF PRD cccccceeeccccccceeeeecccccccceeeeeccccchhhhhhhhhhhccceeeeecc

SEQ RRARPKFEQVNLLDSNAVHHIIHDFQPHVIVHCAAERRPDVVENQPDAASQLNVDASGNL PRD cccccccccccccchhhhhhhhhhhccceeeehhhhhhhhhhhhhhhhhhhhhhccchhh

SEQ AKEAAAVGAFLIYISSDYVFDGTNPPYREEDIPAPLNLYGKTKLDGEKAVLENNLGAAVL PRD hhhhhhhhheeeeeeccccccccccccccccccccccccchhhhhhhhhccccccceeee

SEQ RIPILYGEVEKLEESAVTVMFDKVQFSNKSANMDHWQQRFPTHVKDVATVCRQLAEKRML

PRD eeeeeecccccccchhhhhhhhhhhhhccceeeccccccccccchhhhhhhhhhhhhhhh

SEQ DPSIKGTFHWSGNEQMTKYEMACAIADAFNLPSSHLRPITDSPVLGAQRPRNAQLDCSKL PRD cccccceeeeccccccchhhhhhhhhhhhhcccccccccccccccccccccccchhhhhh

SEQ ETLGIGQRTPFRIGIKESLWPFLIDKRWRQTVFH PRD hhhhccccchhhhhhhhhhhhhhhhhhhhhcccc

Prosite for DKFZphfbr2_6b24.1

PS00001 208->212 ASN_GLYCOSYLATION PDOC00001 PS00005 16->19 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 207->210 PKC_PHOSPHO_SITE PDOC00005 PS00005 243->246 PKC_PHOSPHO_SITE PDOC00005 PS00006 162->166 CK2_PHOSPHO_SITE PDOC00006 PS00006 251->255 CK2_PHOSPHO_SITE PDOC00006 PS00006 257->261 CK2_PHOSPHO_SITE PDOC00006 PS00006 298->302 CK2_PHOSPHO_SITE PDOC00006 PS00008 314->320 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_6b24.1)

DKFZphfbr2_6ι20

group: brain derived

DKFZphfbr2_6ι20 encodes a novel 296 ammo acid protein with similarity to ribosomal protein L15 precursor of S. cerevisiae mitochondria.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . similarity to ribosomal protein L15 precursor, mitochondrial complete cDNA, complete eds, EST hits potential miochondrial L15 ribosomal protein

Sequenced by AGOWA

Locus: /map="377.5 cR from top of Chr8 linkage group"

Insert length: 1122 bp

Poly A stretch at pos. 1099, polyadenylation signal at pos. 1071

1 GGGGGCCCTT GAAAGTTCTT GGATCTGCGG GTTATGGCCG GTCCCTTGCA 51 GGGCGGTGGG GCCCGGGCCC TGGACCTACT CCGGGGCCTG CCGCGTGTGA

101 GCCTGGCCAA CTTAAAGCCG AATCCCGGCT CCAAGAAACC GGAGAGAAGA

151 CCAAGAGGTC GGAGAAGAGG TAGAAAATGT GGCAGAGGCC ATAAAGGAGA

201 AAGGCAAAGA GGAACCCGGC CCCGCTTGGG CTTTGAGGGA GGCCAGACTC

251 CATTTTACAT CCGAATCCCA AAATACGGGT TTAACGAAGG ACATAGTTTC

301 AGACGCCAGT ATAAGCCTAT GAGTCTCAAT AGACTGCAGT ATCTTATTGA

351 TTTGGGTCGT GTTGATCCTA GTCAACCTAT TGACTTAACC CAGCTTGTCA

401 ATGGGAGAGG TGTGACCATC CAGCCACTTA AAAGGGATTA TGATGTCCAG

451 CTGGTTGAGG AGGGTGCTGA CACCTTTACG GCAAAAGTTA ATATTGAAGT

501 ACAGTTGGCT TCAGAACTAG CTATTGCTGC CATTGAAAAA AATGGTGGTG

551 TTGTTACTAC AGCCTTCTAT GATCCAAGAA GTCTGGACAT TGTATGCAAA

601 CCTGTTCCAT TCTTTCTTCG TGGACAACCC ATTCCAAAAA GAATGCTTCC

651 ACCAGAAGAA CTGGTACCAT ATTACACTGA TGCAAAGAAC CGTGGGTACC

701 TGGCGGATCC TGCCAAATTT CCTGAAGCAC GACTTGAACT CGCCAGGAAG

751 TATGGTTATA TCTTACCTGA TATCACTAAA GATGAACTCT TCAAAATGCT

801 CTGTACTAGG AAGGATCCAA GGCAGATTTT CTTTGGTCTT GCTCCAGGAT

851 GGGTGGTGAA TATGGCCGAT AAGAAAATCC TAAAACCTAC AGATGAAAAT

901 CTCCTTAAGT ATTATACCTC ATGAATTCCC GTCCAAGGAA GCAGAGTTGT

951 TAAAGAGTAC TGGAATAGGG GCTGAAGGAT CTATATTCCC TTATTGCATT 1001 TTCCTTATGT ATAATTTTCC AGATGGTGAT GTTACTTTTC AGTGTACTCA 1051 TATGTCTCAT TTTCATCTAA AATTAAATGG CAGGAAACAA GGACTGCATA 1101 GAGAAAAAAA AAAAAAAAAA AA

BLAST Results

Entry HΞ500354 from database EMBL: human STS WI-12392.

Length = 426

Minus Strand HSPs:

Score = 1791 (268.7 bits), Expect = l.le-74, P = l.le-74

Identities = 375/384 (97%)

Medline entries

No Medline entry

Peptide information for frame 1

ORF from 34 bp to 921 bp; peptide length: 296 Category: strong similarity to known protein

1 MAGPLQGGGA RALDLLRGLP RVSLANLKPN PGSKKPERRP RGRRRGRKCG 51 RGHKGERQRG TRPRLGFEGG QTPFYIRIPK YGFNEGHSFR RQYKPMSLNR 101 LQYLIDLGRV DPSQPIDLTQ LVNGRGVTIQ PLKRDYDVQL VEEGADTFTA 151 KVNIEVQLAS ELAIAAIEKN GGVVTTAFYD PRSLDIVCKP VPFFLRGQPI 201 PKRMLPPEEL VPYYTDAKNR GYLADPAKFP EARLELARKY GYILPDITKD 251 ELFKMLCTRK DPRQIFFGLA PGWVVNMADK KILKPTDENL LKYYTS

BLASTP hits

Entry S63258 from database PIR: ribosomal protein L15 precursor, mitochondrial - yeast (Saccharomyces cerevisiae)

Length = 322

Score = 259 (91.2 bits), Expect = 2.0e-22, P = 2.0e-22

Identities = 71/200 (35%), Positives = 106/200 (53%)

Entry H70161 from database PIR: ribosomal protein L15 (rplO) - Lyme disease spirochete

Length = 145

Score = 173 (60.9 bits), Expect = 4.8e-13, P = 4.8e-13

Identities = 45/140 (32%), Positives = 73/140 (52%)

Alert BLASTP hits for DKFZphfbr2_6ι20, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_6ι20, frame 1

Report for DKFZphfbr2_6ι20.1

[LENGTH] 296

[MW] 33495.98

[pi] 9.98

[HOMOL] TREMBL :AF067212_1 gene: "F37F2.1"; Caenorhabditis elegans cosmid F37F2. le-3.

[FUNCAT] 05.01 ribosomal proteins [S. cerevisiae, YNL284c] 7e-15

[FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YNL284C] 7e-15

[FUNCAT] j mrna translation and ribosome biogenesis [M. genitalium, MG169] le-06

[BLOCKS] BL00475D

[BLOCKS] BL00475B Ribosomal protein L15 proteins

[PIRKW] ribosome 2e-13

[PIRKW] mitochondrion 2e-13

[PIRKW] protein biosynthesis 2e-13

[SUPFAM] Escherichia coll ribosomal protein L15 4e-06

[PROSITE] MYRISTYL 3

[PROSITE] AMIDATION 2

[PROSITE] CK2_PHOSPHO_SITE 2

[PROSITE] PKC_PHOSPHO_SITE 4

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 12.50 %

SEQ MAGPLQGGGARALDLLRGLPRVSLANLKPNPGΞKKPERRPRGRRRGRKCGRGHKGERQRG SEG xxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxx... PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ TRPRLGFEGGQTPFYIRlPKYGFNEGHSFRRQYKPMSLNRLQYLIDLGRVDPSQPIDLTQ SEG PRD ccccccccccccceeeeeccccccccccccccccccchhhhhhhhhccccccccccccee

SEQ LVNGRGVTIQPLKRDYDVQLVEEGADTFTAKVNIEVQLASELAIAAIEKNGGVVTTAFYD SEG PRD ecccceeeeccccccceeeeeeccccccchhhhhhhhhhhhhhhhhhhhccceeeeeecc

SEQ PRSLDIVCKPVPFFLRGQPIPKRMLPPEELVPYYTDAKNRGYLADPAKFPEARLELARKY SEG PRD ccccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhh

SEQ GYILPDITKDELFKMLCTRKDPRQIFFGLAPGWVVNMADKKILKPTDENLLKYYTS SEG PRD cccccccchhhhhhhhhcccccceeeeeccccceeeeccceeecccchhhhhcccc

Prosite for DKFZphfbr2_6ι20.1

PS00005 33->36 PKC_PHOSPHO_SITE PDOC00005 PS00005 88->91 PKC PHOSPHO SITE PDOC00005 PS00005 149->152 PKC_PHOSPHO_SITE PDOC00005 PS00005 258->261 PKC_PHOSPHO_SITE PDOC00005 PS00006 248->252 CK2_PHOSPHO_SITE PDOC00006 PS00006 258->262 CK2_PHOSPHO_SITE PDOC00006 PS00008 8->14 MYRISTYL PDOC00008 PS00008 171->177 MYRISTYL PDOC00008 PS00008 268->274 MYRISTYL PDOC00008 PS00009 41->45 AMIDATION PDOC00009 PS00009 45->49 AMIDATION PDOC00009

(No Pfam data available for DKFZphfbr2_6ι20.1)

DKFZphfbr2_6ol7

group: nucleic acid management

DKFZphfbr2_6ol7 encodes a novel 455 ammo acid protein with strong similarity to DEAD-box ATP- dependent RNA helicases YHR065c and T26G10.1.

The S. cerevisiae protein YHR065c is required for maturation of the 35S RNA primary transcript .

The new protein can find application in modulating rRNA maturation. strong similar to RNA helicases complete cDNA, complete eds, EST hits probable start at Bp 27 matchs kozak consensus ANNatgG involved in maturation of r-RNA "

YHR065c/Rrp3p is involved in maturation of the 35S primary transcript

Drslp cold-sensitive mutation has slow 27S to 25S pre-rRNA conversion and is deficient m 60S ribosomal subunits

Sequenced by AGOWA

Locus : unknown

Insert length: 1840 bp

Poly A stretch at pos. 1815, polyadenylation signal at pos. 1793

1 GGGGACTTCC GGAGACCTCA CACAAGATGG CGGCACCCGA GGAACACGAT

51 TCTCCGACCG AAGCGTCCCA GCCGATTGTG GAAGAGGAGG AAACTAAAAC

101 ATTTAAAGAC CTGGGTGTGA CAGATGTGTT GTGTGAAGCT TGTGACCAGT

151 TGGGATGGAC AAAACCCACC AAGATTCAGA TTGAAGCTAT TCCTTTGGCC

201 TTACAAGGTC GTGATATCAT TGGGCTTGCA GAAACTGGCT CTGGAAAGAC

251 AGGCGCCTTT GCTTTGCCCA TTCTAAACGC ACTGCTGGAG ACCCCGCAGC

301 GTTTGTTTGC CCTAGTTCTT ACCCCGACTC GGGAGCTGGC CTTTCAGATC

351 TCAGAGCAGT TTGAAGCCCT GGGGTCCTCT ATTGGAGTGC AGAGTGCTGT

401 GATTGTAGGT GGAATTGATT CAATGTCTCA ATCTTTGGCC CTTGCAAAAA

451 AACCACATAT AATAATAGCA ACTCCTGGTC GACTGATTGA CCACTTGGAA

501 AATACGAAAG GTTTCAACTT GAGAGCTCTC AAATACTTGG TCATGGATGA

551 AGCCGACCGA ATACTGAATA TGGATTTTGA GACAGAGGTT GACAAGATCC

601 TCAAAGTGAT TCCTCGAGAT CGGAAAACAT TCCTCTTCTC TGCCACCATG

651 ACCAAGAAGG TTCAAAAACT TCAGCGAGCA GCTCTGAAGA ATCCTGTGAA

701 ATGTGCCGTT TCCTCTAAAT ACCAGACAGT TGAAAAATTA CAGCAATATT

751 ATATTTTTAT TCCCTCTAAA TTCAAGGATA CCTACCTGGT TTATATTCTA

801 AATGAATTGG CTGGAAACTC CTTTATGATA TTCTGCAGCA CCTGTAATAA

851 TACCCAGAGA ACAGCTTTGC TACTGCGAAA TCTTGGCTTC ACTGCCATCC

901 CCCTCCATGG ACAAATGAGT CAGAGTAAGC GCCTAGGATC CCTTAATAAG

951 TTTAAGGCCA AGGCCCGTTC CATTCTTCTA GCAACTGACG TTGCCAGCCG

1001 AGGTTTGGAC ATACCTCATG TAGATGTGGT TGTCAACTTT GACATTCCTA

1051 CCCATTCCAA GGATTACATC CATCGAGTAG GTCGAACAGC TAGAGCTGGG

1101 CGCTCCGGAA AGGCTATTAC TTTTGTCACA CAGTATGATG TGGAACTCTT

1151 CCAGCGCATA GAACACTTAA TTGGGAAGAA ACTACCAGGT TTTCCAACAC

1201 AGGATGATGA GGTTATGATG CTGACAGAAC GCGTCGCTGA AGCCCAAAGG

1251 TTTGCCCGAA TGGAGTTAAG GGAGCATGGA GAAAAGAAGA AACGCTCGCG

1301 AGAGGATGCT GGAGATAATG ATGACACAGA GGGTGCTATT GGTGTCAGGA

1351 ACAAGGTGGC TGGAGGAAAA ATGAAGAAGC GGAAAGGCCG TTAATCACTT

1401 TTATGAAGGC TCGAGTTCTG CTGTTCTGTA AAAGAAAATT GGAGAATGAA

1451 ACCTGCTCCA ACAGAGATCA TGAGACTGAA ATTGGTCAGA ATTGTGTCCA

1501 GAATGTGCTC AGCTAATTCA GTATTCTTCC CCATTCTGGG TTGGAGTTTA

1551 CTGCAGAGTA ATTCTTACAG TGCTGATGTC AAGACTGTTA CTGTTCTTCG

1601 ACTTTGATTC CTTGCTCATG ACATGAGTAG GGTGTGCTCT TCTGTCACTT

1651 CACACAGACC TTTTGCCTTT TTTAGCTGCA AGTCAAGGAC TAGGTTGATG

1701 ATGCCCATGA CCTGTAATTG TAAAGAAGCT TGGACATCTG CAAATGATAT

1751 TTAAACCATC TTGGCTTGTG CTTTATTCAA ACTAATGTGA AACAATAAAT

1801 TTAAATATTA TTTTTAAAAG AAAAAAAAAA AAAAAAAAAA

BLAST Results o BLAST result

Medline entries o Medlme entry Peptide information for frame 3

ORF from 27 bp to 1391 bp; peptide length: 455 Category: strong similarity to known protein

1 MAAPEEHDSP TEASQPIVEE EETKTFKDLG VTDVLCEACD QLGWTKPTKI

51 QIEAIPLALQ GRDIIGLAET GSGKTGAFAL PILNALLETP QRLFALVLTP

101 TRELAFQISE QFEALGSSIG VQSAVIVGGI DSMSQSLALA KKPHIIIATP

151 GRLIDHLENT KGFNLRALKY LVMDEADRIL NMDFETEVDK ILKVIPRDRK

201 TFLFSATMTK KVQKLQRAAL KNPVKCAVSS KYQTVEKLQQ YYIFIPSKFK

251 DTYLVYILNE LAGNSFMIFC STCNNTQRTA LLLRNLGFTA IPLHGQMSQS

301 KRLGSLNKFK AKARSILLAT DVASRGLDIP HVDVVVNFDI PTHSKDYIHR

351 VGRTARAGRΞ GKAITFVTQY DVELFQRIEH LIGKKLPGFP TQDDEVMMLT

401 ERVAEAQRFA RMELREHGEK KKRΞREDAGD NDDTEGAIGV RNKVAGGKMK

451 KRKGR

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_6ol7, frame 3

PIR:S40731 ATP-dependent RNA helicase homolog T26G10.1 - Caenorhabditis elegans, N = 1, Score = 1497, P = 1.6e-153

PIR:S46713 hypothetical protein YHR065c - yeast (Saccharomyces cerevisiae), N = 1, Score = 1154, P = 3.6e-117

TREMBL :ATH010462_1 gene: "RH10"; product: "RNA helicase"; Arabidopsis thaliana mRNA for DEAD box RNA helicase, RH10, N = 1, Score = 1122, P = 8.9e-114

TREMBL :AC002985_2 product: "R27090_2"; Human DNA from chromosome 19-specιfιc cosmid R27090, genomic sequence, complete sequence., N = 1, Score = 950, P = 1.5e-95

>PIR:S40731 ATP-dependent RNA helicase homolog T26G10.1 - Caenorhabditis elegans

Length = 489

HSPs:

Score = 1497 (224.6 bits), Expect = 1.6e-153, P = 1.6e-153 Identities = 283/442 (64%), Positives = 364/442 (82%)

Query: 19 EEEETKTFKDLGVTDVLCEACDQLGWTKPTKIQIEAIPLALQGRDIIGLAETGSGKTGAF 78

E+ + K+F +LGV+ LC+AC +LGW KP+KIQ A+P ALQG+D+IGLAETGSGKTGAF Sbjct: 39 EDVKEKSFAELGVSQPLCDACQRLGWMKPSKIQQAALPHALQGKDVIGLAETGSGKTGAF 98

Query: 79 ALPILNALLETPQRLFALVLTPTRELAFQISEQFEALGSSIGVQSAVIVGGIDSMSQΞLA 138

A+P+L +LL+ PQ F LVLTPTRELAFQI +QFEALGS IG+ +AVIVGG+D +Q++A Sbjct: 99 AIPVLQSLLDHPQAFFCLVLTPTRELAFQIGQQFEALGSGIGLIAAVIVGGVDMAAQAMA 158

Query: 139 LAKKPHIIIATPGRLIDHLENTKGFNLRALKYLVMDEADRILNMDFETEVDKILKVIPRD 198

LA++PHII+ATPGRL+DHLENTKGFNL+ALK+L+MDEADRILNMDFE E+DKILKVIPR+ Sbjct: 159 LARRPHIIVATPGRLVDHLENTKGFNLKALKFLIMDEADRILNMDFEVELDKILKVIPRE 218

Query: 199 RKTFLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYIFIPSKFKDTYLVYIL 258

R+T+LFSATMTKKV KL+RA+L++P + +VSS+Y+TV+ L+Q+YIF+P+K+K+TYLVY+L Sbjct: 219 RRTYLFSATMTKKVSKLERASLRDPARVSVSSRYKTVDNLKQHYIFVPNKYKETYLVYLL 278

Query: 259 NELAGNSFMIFCSTCNNTQRTALLLRNLGFTAIPLHGQMSQSKRLGΞLNKFKAKARSILL 318

NE AGNS ++FC+TC T + A++LR LG A+PLHGQMΞQ KRLGSLNKFK+KAR IL+ Sbjct: 279 NEHAGNSAIVFCATCATTMQIAVMLRQLGMQAVPLHGQMSQEKRLGSLNKFKSKAREILV 338

Query: 319 ATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRSGKAITFVTQYDVELFQRI 378

TDVA+RGLDIPHVD+V+N+D+P+ SKDY+HRVGRTARAGRSG AIT VTQYDVE +Q+I Sbjct: 339 CTDVAARGLDIPHVDMVINYDMPSQSKDYVHRVGRTARAGRSGIAITVVTQYDVEAYQKI 398

Query: 379 EHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEKKK RSREDAGDNDD 433

E +GKKL + ++EVM+L ER EA AR+E++E EKKK R +D GD ++ Sbjct: 399 EANLGKKLDEYKCVENEVMVLVERTQEATENARIEMKEMDEKKKSGKKRRQNDDFGDTEE 458

Query: 434 TEGAIGVRNKVAGGKMKKRKGR 455 + G + K GG+ GR Sbjct: 459 SGGRFKMGIKSMGGRGGSGGGR 480

Pedant information for DKFZphfbr2_6ol7, frame 3

Report for DKFZphfbr2_6ol7.3

[LENGTH] 455

[MW] 50646.80

[pi] 9.18

[HOMOL] PIR:S40731 ATP-dependent RNA helicase homolog T26G10.1 - Caenorhabditis elegans le-167

[FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YHR065C] le-127

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YHR065c] le-127

[FUNCAT] 04.99 other transcription activities [Ξ. cerevisiae, YHR169w] 2e-79

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YLLOOδw] le-71

[FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YMR290c] 4e-66

[FUNCAT] j mrna translation and ribosome biogenesis [H. influenzae, HI0231 RNA] le-63

[FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YJL033w] le-58

[FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YDL084w] le-55

[FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae,

YOR204w] 5e- 55

[ FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YOR204w] 5e-55

[FUNCAT] 1 genome replication, transcription, recombination and repair [H. influenzae, HI0892] 9e-48

[FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YLR276c] 2e-45

[FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YDR194c] 4e-42

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YGL064c] 7e-16

[ FUNCAT ] 03.19 recombination and dna repair [S. cerevisiae, YMR190c] 7e-12

[FUNCAT] 11.10 cell death [S. cerevisiae, YMR190c] 7e-12

[ FUNCAT ] r general function prediction [M. jannaschn, MJ1401] 5e-06

[BLOCKS] BL00175B Phosphoglycerate mutase family phosphohistidme proteins

[BLOCKS] BL00039D DEAD-box subfamily ATP-dependent helicases proteins

[BLOCKS] BL00039C DEAD-box subfamily ATP-dependent helicases proteins

[BLOCKS] BL00039B DEAD-box subfamily ATP-dependent helicases proteins

[BLOCKS] BL00039A DEAD-box subfamily ATP-dependent helicases proteins

[PIRKW] nucleus 4e-60

[PIRKW] RNA binding 7e-69

[PIRKW] DEAD box 7e-69

[PIRKW] transmembrane protein 9e-41

[PIRKW] DNA binding 3e-55

[PIRKW] recF recombination pathway 3e-ll

[PIRKW] ATP le-126

[PIRKW] purme nucleotide binding 7e-69

[PIRKW] P-loop le-126

[PIRKW] hydrolase le-55

[PIRKW] protein biosynthesis 7e-69

[PIRKW] ATP binding 3e-61

[SUPFAM] ATP-dependent RNA helicase eIF-4A 8e-06

[SUPFAM] WW repeat homology 4e-58

[SUPFAM] translation initiation factor eIF-4A 7e-69

[SUPFAM] DEAD/H box helicase homology le-126

[SUPFAM] recQ helicase homology 5e-12

[SUPFAM] ATP-dependent RNA helicase homology 8e-06

[SUPFAM] unassigned DEAD/H box helicases le-126

[SUPFAM] ATP-dependent RNA helicase DBP1 4e-60

[SUPFAM] ATP-dependent RNA helicase DHH1 le-58

[SUPFAM] recQ protein 3e-ll

[SUPFAM] tobacco ATP-dependent RNA helicase DB10 4e-58

[SUPFAM] Bloom's syndrome helicase 5e-12

[PROSITE] DEAD_ATP_HELICASE 1

[PROSITE] ATP_GTP_A 1

[PROSITE] MYRISTYL 5

[PROSITE] AMIDATION 1

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOΞPHO_SITE 6

[PROSITE] PKC_PHOSPHO_SITE 9

[PROSITE] ASN_GLYCOSYLATION 1

[PFAM] Helicases conserved C-terminal domain

[PFAM] DEAD and DEAH box helicases

[KW] Alpha_Beta

SEQ MAAPEEHDSPTEASQPIVEEEETKTFKDLGVTDVLCEACDQLGWTKPTKIQIEAIPLALQ

PRD cccccccccccccccchhhhhhhhhhhccccchhhhhhhhhhcccccccccccccccccc

SEQ GRDIIGLAETGSGKTGAFALPILNALLETPQRLFALVLTPTRELAFQISEQFEALGSSIG

PRD ccceeeeeccccccceeehhhhhhhhcccccceeeeeeccchhhhhhhhhhhhhhhhhcc SEQ VQSAVIVGGIDSMSQSLALAKKPHIIIATPGRLIDHLENTKGFNLRALKYLVMDEADRIL PRD eeeeeeeccchhhhhhhhhhccceeeeeccccccccccccccccccccceeehhhhhhhh

SEQ NMDFETEVDKILKVIPRDRKTFLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQ PRD hhcchhhhhhhhhhcccchhhhhhhhccchhhhhhhhhhhccceeeeeecccccchhhhh

SEQ YYIFIPSKFKDTYLVYILNELAGNSFMIFCSTCNNTQRTALLLRNLGFTAIPLHGQMSQS PRD hhhhhhhhhhhhhhhhhhhhhccceeeeeeecchhhhhhhhhhhhcccceeeccccchhh

SEQ KRLGSLNKFKAKARSILLATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRS PRD hhhhhhhhhhhhhhhcchhhhhhhhcccccceeeeeecccccccceeeeecccccccccc

SEQ GKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEK PRD cceeeeeecchhhhhhhhhhhhhhhhccccccchhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ KKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR PRD hhhhccccccccccccccccccccccccccccccc

Prosite for DKFZphfbr2_6ol7.3

PS00001 274->278 ASN_GLYCOSYLATION PDOC00001

PS00004 421->425 CAMP_PHOSPHO_SITE PDOC00004

PS00005 25->28 PKC_PHOSPHO_SITE PDOC00005

PS00005 72->75 PKC_PHOSPHO_SITE PDOC00005

PS00005 209->212 PKC_PHOSPHO_SITE PDOC00005

PS00005 229->232 PKC_PHOSPHO_SITE PDOC00005

PS00005 276->279 PKC_PHOSPHO_SITE PDOC00005

PS00005 300->303 PKC_PHOSPHO_SITE PDOC00005

PS00005 354->357 PKC_PHOSPHO_SITE PDOC00005

PS00005 360->363 PKC_PHOSPHO_SITE PDOC00005

PS00005 400->403 PKC_PHOSPHO_SITE PDOC00005

PS00006 9->13 CK2_PHOSPHO_SITE PDOC00006

PS00006 25->29 CK2_PHOSPHO_SITE PDOC00006

PS00006 186->190 CK2_PHOSPHO_SITE PDOC00006

PS00006 368->372 CK2_PHOSPHO_SITE PDOC00006

PS00006 391->395 CK2_PHOSPHO_SITE PDOC00006

PS00006 424->428 CK2_PHOSPHO_SITE PDOC00006

PS00008 66->72 MYRISTYL PDOC00008

PS00008 71->77 MYRISTYL PDOC00008

PS00008 116->122 MYRISTYL PDOC00008

PS00008 120->126 MYRISTYL PDOC00008

PS00008 128->134 MYRISTYL PDOC00008

PS00009 382->386 AMIDATION PDOC00009

PS00017 68->76 ATP_GTP_A PDOC00017

PS00039 172->181 DEAD ATP HELICASE PDOC00039

Pfam for DKFZphfbr2_6ol7.3

HMM_NAME DEAD and DEAH box helicases

HMM *gLpPWILRnIyeMGFEkPTPIQQqAIPιILeGRDVMACAQTGSGKTAAF G ++ ++++++++G++KPT+IQ +AIP++L+GRD+++ A TGSGKT+AF

Query 30 GVTDVLCEACDQLGWTKPTKIQIEAIPLALQGRDIIGLAETGSGKTGAF

HMM HPMLQHIDwdPWpqpPQdPrALILAPTRELAMQIQEEcRkFgkHMnglR ++P+L ++++P + ++AL+L+PTRELA QI+E+++++G++++ ++

Query 79 ALPILNALLETP QR-LFALVLTPTRELAFQISEQFEALGSSIG-VQ 122

HMM ImcIYGGtnMRdQMRmLeRGpPHIVIATPGRLIDHIER.gtldLDrleML +++I+GG + + Q L+++P HI+IATPGRLIDH+E+ ++L+++++L

Query 123 SAVIVGGIDSMSQSLALAKKP-HIIIATPGRLIDHLENTKGFNLRALKYL 171

HMM VMDEADRMLDMGFIDQIRrlMrqIPMpwNRQTMMFSATMPdelqELARrF VMDEADR+L+M+F+ ++++I++ IP ++R T +FSATM++++Q+L+R+

Query 172 VMDEADRILNMDFETEVDKILKVIP—RDRKTFLFSATMTKKVQKLQRAA 219

HMM MRNPIRInldMdElTtnEnlkQwYiyVerEMWKfdcLcrLIe* ++NP+ ++ ++++T++ ++Q+YI+++ + K +L+++++

Query 220 LKNPVKCAVSSKYQTVE-KLQQYYIFIP-SKFKDTYLVYILN 259

HMM_NAME Helicases conserved C-terminal domain

HMM *EιleeWLknlGIrvmYIHGdMpQeERdeIMddFNnGEynVLIcTDVggR ++ + L+NLG++++ +HG+M+Q +R+ +++F++ +L++TDV++R Query 277 QRTALLLRNLGFTAIPLHGQMSQSKRLGSLNKFKAKARSILLATDVAΞR 325

HMM GIDIPdVNHVINYDMPWNPEqYIQRIGRTgRIG*

G+DIP V++V+N+D+P ++ +YI+R+GRT+R+G Query 326 GLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAG 358

DKFZphfbr2_71o20

group: brain derived

DKFZphfbr2_71o20 encodes a novel 232 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . unknown complete cDNA, complete eds, EST hits on genomic level encoded by AC006186 (3 exons)

Sequenced by GBF

Locus: /map="10q22.1"

Insert length: 1768 bp

Poly A stretch at pos. 1742, polyadenylation signal at pos. 1726

1 GGGGGCAGCA GGCCAAGGGG GAGGTGCGAG CGTGGACCTG GGACGGGTCT

51 GGGCGGCTCT CGGTGGTTGG CACGGGTTCG CACACCCATT CAAGCGGCAG

101 GACGCACTTG TCTTAGCAGT TCTCGCTGAC CGCGCTAGCT GCGGCTTCTA

151 CGCTCCGGCA CTCTGAGTTC ATCAGCAAAC GCCCTGGCGT CTGTCCTCAC

201 CATGCCTAGC CTTTGGGACC GCTTCTCGTC GTCGTCCACC TCCTCTTCGC

251 CCTCGTCCTT GCCCCGAACT CCCACCCCAG ATCGGCCGCC GCGCTCAGCC

301 TGGGGGTCGG CGACCCGGGA GGAGGGGTTT GACCGCTCCA CGAGCCTGGA

351 GAGCTCGGAC TGCGAGTCCC TGGACAGCAG CAACAGTGGC TTCGGGCCGG

401 AGGAAGACAC GGCTTACCTG GATGGGGTGT CGTTGCCCGA CTTCGAGCTG

451 CTCAGTGACC CTGAGGATGA ACACTTGTGT GCCAACCTGA TGCAGCTGCT

501 GCAGGAGAGC CTGGCCCAGG CGCGGCTGGG CTCTCGACGC CCTGCGCGCC

551 TGCTGATGCC TAGCCAGTTG GTAAGCCAGG TGGGCAAAGA ACTACTGCGC

601 CTGGCCTACA GCGAGCCGTG CGGCCTGCGG GGGGCGCTGC TGGACGTCTG

651 CGTGGAGCAG GGCAAGAGCT GCCACAGCGT GGGCCAGCTG GCACTCGACC

701 CCAGCCTGGT GCCCACCTTC CAGCTGACCC TCGTGCTGCG CCTGGACTCA

751 CGACTCTGGC CCAAGATCCA GGGGCTGTTT AGCTCCGCCA ACTCTCCCTT

801 CCTCCCTGGC TTCAGCCAGT CCCTGACGCT GAGCACTGGC TTCCGAGTCA

851 TCAAGAAGAA GCTGTACAGC TCGGAACAGC TGCCCATTGA GGAGTGTTGA

901 ACTTCAACCT GAGGGGGCCG ACAGTGCCCT CCAAGACAGA GACGACTGAA

951 CTTTTGGGGT GGAGACTAGA GGCAGGAGCT GAGGGACTGA TTCCAGTGGT

1001 TGGAAAACTG AGGCAGCCAC CTAAAGTGGA GGTGGGGGAA TAGTGTTTCC

1051 CAGGAAGCTC ATTGAGTTGT GTGCGGGTGG CTGTGCATTG GGGACACATA

1101 CCCCTCAGTA CTGTAGCATG AAACAAAGGC TTAGGGGCCA ACAAGGCTTC

1151 CAGCTGGATG TGTGTGTAGC ATGTACCTTA TTATTTTTGT TACTGACAGT

1201 TAACAGTGGT GTGACATCCA GAGAGCAGCT GGGCTGCTCC CGCCCCAGCC

1251 TGGCCCAGGG TGAAGGAAGA GGCACGTGCT CCTCAGAGCA GCCGGAGGGA

1301 AGGGGGAGGT CGGAGGTCGT GGAGGTGGTT TGTGTATCTT ACTGGTCTGA

1351 AGGGACCAAG TGTGTTTGTT GTTTGTTTTG TATCTTGTTT TTCTGATCGG

1401 AGCATCACTA CTGACCTGTT GTAGGCAGCT ATCTTACAGA CGCATGAATG

1451 TAAGAGTAGG AAGGGGTGGG TGTCAGGGAT CACTTGGGAT CTTTGACACT

1501 TGAAAAATTA CACCTGGCAG CTGCGTTTAA GCCTTCCCCC ATCGTGTACT

1551 GCAGAGTTGA GCTGGCAGGG GAGGGGCTGA GAGGGTGGGG GCTGGAACCC

1601 CTTCCCGGGA GGAGTGCCAT CTGGGTCTTC CATCTAGAAC TGTTTACATG

1651 AAGATAAGAT ACTCACTGTT CATGAATACA CTTGATGTTC AAGTATTAAG

1701 ACCTATGCAA TATTTTTTAC TTTTCTAATA AACATGTTTG TTAAAACAAA

1751 AAAAAAAAAA AAAAAAAA

BLAST Results

Entry AC006186 from database EMBLNEW:

*** SEQUENCING IN PROGRESS *** Homo sapiens chromosome 10 clone

CRI-JC2048 map 10q22.1; HTGS phase 1, 4 unordered pieces.

Score = 6512, P = O.Oe+00, identities = 1326/1345

3 exons

Medlme entries

No Medlme entry Peptide information for frame 1

ORF from 202 bp to 897 bp; peptide length: 232 Category: putative protein

1 MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE

51 SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL

101 QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC

151 VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF

201 LPGFSQSLTL STGFRVIKKK LYSSEQLPIE EC

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_71o20, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_71o20, frame 1

Report for DKFZphfbr2_71o20.1

[LENGTH] 232

[MW] 25354.60

[pi] 4.87

[PROSITE] MYRISTYL 2

[PROSITE] CK2_PHOSPHO_SITE 6

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE 1

[KW] All_Alpha

[KW] LOW COMPLEXITY 17.67

SEQ MPSLWDRFSSSSTSΞSPSSLPRTPTPDRPPRSAWGSATREEGFDRSTSLESSDCESLDSS

SEG xxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxx

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ NSGFGPEEDTAYLDGVSLPDFELLΞDPEDEHLCANLMQLLQESLAQARLGSRRPARLLMP SEG XX PRD cccccccccccccccccccceeeccccccchhhhhhhhhhhhhhhhhhccccccceeecc

SEQ SQLVSQVGKELLRLAYSEPCGLRGALLDVCVEQGKSCHSVGQLALDPSLVPTFQLTLVLR SEG PRD ccccchhhhhhhhhhhcccccchhhhhhhhccccccccccccccccccccchhhhhhccc

SEQ LDSRLWPKIQGLFSSANSPFLPGFSQSLTLSTGFRVIKKKLYSSEQLPIEEC SEG PRD cccccccccccccccccccccccccceeeecccccccccccccccccccccc

Prosite for DKFZphfbr2_71o20.1

PS00002 62->66 GLYCOSAMINOGLYCAN PDOC00002

PS00005 111->114 PKC_PHOSPHO_SITE PDOC00005

PS00006 3->7 CK2_PHOSPHO_SITE PDOC00006

PS00006 38->42 CK2_PHOSPHO_SITE PDOC00006

PS00006 47->51 CK2_PHOSPHO_SITE PDOC00006

PS00006 52->56 CK2_PHOΞPHO_SI E PDOC00006

PS00006 77->81 CK2_PHOSPHO_SITE PDOC00006

PS00006 85->89 CK2_PHOSPHO_SITE PDOC00006

PS00008 141->147 MYRISTYL PDOC00008

PS00008 191->197 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_71o20.1) DKFZphfbr2_72bl8 group: nucleic acid management

DKFZphfbr2_72bl8 encodes a novel 715 amino acid protein w th similarity to E. coli DNA-damage- mducibile protein dmP and other proteins induced by DNA-damage.

The novel protein is similar to dinP of E. coli, yqjH of B. subtilis, dinP of M. tuberculosis and T19K24.15 of A. thaliana. The dinB/P pathway is a second SOS-pathway in E. coli. Therefore the new gene seems to be involved in DNA repair.

The new protein can find application in modulating DNA repair and mutagenesis. similarity to DNA damage induced genes complete cDNA, complete eds, potential start at Bp 49, EST hits localisation primer site B is missing¹

Sequenced by LMU

Locus: /map="416.0 cR from top of Chrl8 linkage group"??

Insert length: 2475 bp

Poly A stretch at pos. 2452, polyadenylation signal at pos. 2431

1 GGGGGAGGAA GGCGGCGGCG ACGACGAGGA AGACGCCGAG GCCTGGGCCA

51 TGGAACTGGC GGACGTGGGG GCGGCAGCCA GCTCGCAGGG AGTTCATGAT

101 CAAGTGTTGC CCACACCAAA TGCTTCATCC AGAGTCATAG TACATGTGGA

151 TCTGGATTGC TTTTATGCAC AAGTAGAAAT GATCTCAAAT CCAGAGCTAA

201 AAGACAAACC TTTAGGGGTT CAACAGAAAT ATTTGGTGGT TACCTGCAAC

251 TATGAAGCTA GGAAACTTGG AGTTAAGAAA CTTATGAATG TCAGAGATGC

301 AAAAGAAAAG TGTCCACAGT TGGTATTAGT TAATGGAGAA GACCTGACCC

351 GCTACAGAGA AATGTCTTAT AAGGTTACAG AATTACTGGA AGAATTTAGT

401 CCAGTTGTTG AGAGACTTGG ATTTGATGAA AATTTTGTGG ATCTAACAGA

451 AATGGTTGAG AAGAGACTAC AGCAGCTGCA AAGTGATGAA CTTTCTGCGG

501 TGACTGTGTC GGGTCATGTA TACAATAATC AGTCTATAAA CCTGCTTGAC

551 GTCTTGCACA TCAGACTACT TGTTGGATCT CAGATTGCAG CAGAGATGCG

601 GGAAGCCATG TATAATCAGT TGGGGCTCAC TGGCTGTGCT GGAGTGGCTT

651 CTAATAAACT GTTGGCAAAA TTAGTTTCTG GTGTCTTTAA ACCAAATCAA

701 CAAACAGTCT TATTACCTGA AAGTTGTCAA CATCTTATTC ATAGTTTGAA

751 TCACATAAAG GAAATACCTG GTATTGGCTA TAAAACTGCC AAATGTCTTG

801 AAGCACTGGG TATCAATAGT GTGCGTGATC TCCAAACCTT TTCACCCAAA

851 ATTTTAGAAA AAGAATTAGG AATTTCAGTT GCTCAGCGTA TCCAAAAGCT

901 CAGTTTTGGA GAGGATAACT CCCCTGTGAT ACTCTCAGGA CCACCTCAGT

951 CCTTTAGTGA AGAAGATTCA TTTAAAAAAT GTACATCTGA AGTTGAAGCT

1001 AAAAATAAGA TTGAAGAACT ACTTGCTAGT CTTTTAAACA GAGTATGCCA

1051 AGATGGAAGG AAGCCTCATA CAGTGAGATT AATAATCCGT CGGTATTCCT

1101 CTGAGAAGCA CTATGGTCGT GAGAGTCGTC AGTGCCCTAT TCCTTCACAT

1151 GTAATTCAGA AATTAGGGAC AGGAAATTAT GATGTGATGA CCCCAATGGT

1201 TGATATACTT ATGAAACTTT TTCGAAATAT GGTGAATGTG AAGATGCCAT

1251 TTCACCTTAC CCTTCTAAGT GTGTGCTTCT GCAACCTTAA AGCACTAAAT

1301 ACTGCTAAGA AAGGGCTTAT TGATTATTAT TTAATGCCAT CATTATCAAC

1351 TACTTCACGC TCTGGCAAGC ACAGTTTTAA AATGAAAGAC ACTCATATGG

1401 AAGATTTTCC CAAAGACAAA GAAACAAACC GGGATTTCCT ACCAAGTGGA

1451 AGAATTGAAA GTACAAGAAC TAGGGAGTCT CCACTAGATA CCACAAATTT

1501 TTCTAAAGAA AAAGACATTA ATGAATTCCC ACTCTGTTCA CTTCCTGAAG

1551 GTGTTGACCA AGAAGTCTCC AAGCAGCTTC CAGTAGATAT TCAAGAAGAA

1601 ATCCTTTCTG GAAAATCTAG GGAAAAATTT CAAGGGAAAG GAAGTGTGAG

1651 TTGTCCATTA CATGCCTCTA GAGGAGTATT ATCTTTCTTT TCTAAAAAAC

1701 AAATGCAAGA TATTCCCATA AATCCTAGAG ATCATTTATC CAGTAGCAAA

1751 CAGGTATCCT CTGTATCTCC TTGTGAACCG GGAACATCAG GCTTTAATAG

1801 CAGTAGTTCT TCTTACATGT CTAGCCAAAA GGATTATTCA TATTATTTAG

1851 ATAATAGATT AAAAGATGAA CGAATAAGTC AAGGACCTAA AGAACCTCAA

1901 GGATTCCACT TTACAAATTC AAACCCTGCT GTGTCTGCTT TTCATTCATT

1951 TCCAAACTTG CAGAGTGAGC AACTTTTCTC CAGAAACCAC ACTACAGATA

2001 GCCATAAGCA AACAGTAGCA ACAGACTCTC ATGAAGGACT TACAGAAAAT

2051 AGAGAGCCAG ATTCTGTTGA TGAGAAAATT ACTTTCCCTT CTGACATTGA

2101 TCCTCAAGTT TTCTATGAAC TACCAGAAGC AGTACAAAAG GAACTGCTGG

2151 CAGAGTGGAA GAGAACAGGA TCAGATTTCC ACATTGGACA TAAATAAGCA

2201 TATTCAGCAA AAAGGTCTGA AAAGCAAGGG AATACCATTA TTTTCGGATT

2251 AGCGGTTTAT TAAGCTCTTC TATATTAAAC ACTAATAGAT ATTCAATAAC

2301 GGAGTAAACT GTTCCAGATA AAGCAAGAAT AGTTGCAAGA AGTAAATTCT

2351 GGCACAAAGC GTAAAAATAT AACAGAAGAA ATAATGTAAA ATACTATCTT

2401 TTATGTCTAA AGCCATTTTA TATTACTTTT CAATAAAAAG AATATCATGG

2451 TCAAAAAAAA AAAAAAAAAA AAAAC

BLAST Results Entry HS086339 from database EMBL: human STS WI-11064. Score = 1523, P = 3.0e-64, identities = 327/343

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 50 bp to 2194 bp; peptide length: 715 Category: similarity to known protein

1 MELADVGAAA SSQGVHDQVL PTPNASSRVI VHVDLDCFYA QVEMISNPEL

51 KDKPLGVQQK YLVVTCNYEA RKLGVKKLMN VRDAKEKCPQ LVLVNGEDLT

101 RYREMSYKVT ELLEEFSPVV ERLGFDENFV DLTEMVEKRL QQLQSDELSA

151 VTVSGHVYNN QSINLLDVLH IRLLVGSQIA AEMREAMYNQ LGLTGCAGVA

201 SNKLLAKLVS GVFKPNQQTV LLPESCQHLI HΞLNHIKEIP GIGYKTAKCL

251 EALGINSVRD LQTFSPKILE KELGISVAQR IQKLSFGEDN SPVILSGPPQ

301 SFSEEDSFKK CTSEVEAKNK IEELLASLLN RVCQDGRKPH TVRLIIRRYS

351 SEKHYGRESR QCPIPSHVIQ KLGTGNYDVM TPMVDILMKL FRNMVNVKMP

401 FHLTLLSVCF CNLKALNTAK KGLIDYYLMP SLSTTSRSGK HSFKMKDTHM

451 EDFPKDKETN RDFLPSGRIE STRTRESPLD TTNFSKEKDI NEFPLCSLPE

501 GVDQEVSKQL PVDIQEEILS GKSREKFQGK GSVSCPLHAS RGVLSFFSKK

551 QMQDIPINPR DHLSSSKQVS SVSPCEPGTS GFNSSSSSYM SSQKDYSYYL

601 DNRLKDERIS QGPKEPQGFH FTNSNPAVSA FHSFPNLQΞE QLFSRNHTTD

651 SHKQTVATDS HEGLTENREP DSVDEKITFP SDIDPQVFYE LPEAVQKELL

701 AEWKRTGSDF HIGHK

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_72bl8, frame 2

PIR:H64747 DNA-damage-inducibile protein dinP - Escherichia coli, N = 2, Score = 212, P = 4.2e-27

PIR:H69963 DNA-damage repair protein homolog yqjH - Bacillus subtilis, N = 2, Score = 230, P = 5.2e-26

>PIR:H69963 DNA-damage repair protein homolog yqjH - Bacillus subtilis Length = 414

HSPs:

Score = 230 (34.5 bits), Expect = 5.2e-26, Sum P(2) = 5.2e-26 Identities = 47/112 (41%), Positives = 73/112 (65%)

Query: 27 SRVIVHVDLDCFYAQVEMISNPELKDKPLGV QQKYLVVTCNYEARKLGVKKLMNV 81

SR+I H+D++ FYA VEM +P L+ KP+ V ++K +VVTC+YEAR GVK M V Sbjct: 5 SRIIFHIDMNSFYASVEMAYDPALRGKPVAVAGNVKERKGIVVTCSYEARARGVKTTMPV 64

Query: 82 RDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGFDENFVDLTE 134

AK CP+L+++ + RYR S + +L E++ +VE + DE ++D+T+ Sbjct: 65 WQAKRHCPELIVLP-PNFDRYRNSSRAMFTILREYTDLVEPVSIDEGYMDMTD 116

Score = 137 (20.6 bits), Expect = 5.2e-26, Sum P(2) = 5.2e-26 Identities = 43/148 (29%), Positives = 75/148 (50%)

Query: 178 QIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIK 237

+ A E++ + +L L G+A NK LAK+ S + KP T+L ++ L + Sbjct: 125 ETAKEIQSRLQKELLLPSSIGIAPNKFLAKMASDMKKPLGITILRKRQVPDILWPLP-VG 183

Query: 238 EIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGIΞVAQRIQKLSFGEDNSPVILSG 297

E+ G+G KTA+ L+ LGI+++ +L L++ LGI+ R++ + G ++PV Sbjct: 184 EMHGVGKKTAEKLKGLGIHTIGELAAADEHSLKRLLGIN-GPRLKNKANGIHHAPV 238

Query: 298 PPQSFSEEDΞFKKCTSEVEAKNKIEELL 325 P+ E S ++ + EELL Sbj ct : 239 DPERIYEFKSVGNSSTLSHDSSDEEELL 266

Pedant information for DKFZphfbr2_72bl8 , frame 2 Report for DKFZphfbr2_72bl 8 . 2

[ LENGTH] 715

[MW] 80300 . 63

[pi ] 6 . 37

[HOMOL] TREMBL:SPBC16A3_11 gene: "SPBC16A3.il"; product: "hypothetical protein"; S. pombe chromosome II cosmid cl6A3. 5e-30

[FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [Ξ. cerevisiae, YDR419w] 2e-15

[FUNCAT] 1 genome replication, transcription, recombination and repair [M. gemtalium, MG360] 3e-13

[PIRKW] SOS mutagenesis 2e-ll

[PIRKW] DNA repair 2e-ll

[PIRKW] induced mutagenesis 2e-ll

[SUPFAM] umuC protein 3e-29

[PROSITE] MYRISTYL 6

[PROSITE] AMIDATION 1

[PROSITE] CAMP_PHOSPHO_SITE 2

[PROSITE] CK₂_PHOSPHO_SITE 15

[PROSITE] PROKAR_LIPOPROTEIN 1

[PROSITE] TYR_PHOΞPHO_SITE 2

[PROSITE] PKC_PHOSPHO_SITE 21

[PROSITE] ASN_GLYCOSYLATION 5

[KW] Alpha_Beta

[KW] LOW_COMPLEXITY 4.20 %

SEQ MELADVGAAASSQGVHDQVLPTPNASSRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQK SEG

PRD ccceeeeeeecccccceeeccccccceeeeeeeccchhhhhhhhhccccccccceeeecc

SEQ YLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVV SEG

PRD ceeeehhhhhhhhhhcccchhhhhhhhccceeeeccccccchhhhhhhhhhhhhhhccce

SEQ ERLGFDENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLVGSQIA SEG

PRD eeeccchhhhhhhhhhhhhhhhhhccccceeeeeccccccchhhhhhhhhhhhhhhhhhh

SEQ AEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIP SEG

PRD hhhhhhhhhhhcceeeeccchhhhhhhhhhhhhcccceeeeecchhhhhhhhhccccccc

SEQ GIGYKTAKCLEALGINSVRDLQTFSPKILEKELGISVAQRIQKLSFGEDNSPVILSGPPQ SEG

PRD ccchhhhhhhhhhccccchhhhhhhhhhhhhhccchhhhhhhhhhcccccceeeeccccc

SEQ SFSEEDSFKKCTΞEVEAKNKIEELLASLLNRVCQDGRKPHTVRLIIRRYSSEKHYGRESR SEG

PRD ccccccccccchhhhhhhhhhhhhhhhhhhhhhhccccccceeeehhhhhhhhhhhcccc

SEQ QCPIPSHVIQKLGTGNYDVMTPMVDILMKLFRNMVNVKMPFHLTLLSVCFCNLKALNTAK SEG

PRD ccccccceeeeccccccccchhhhhhhhhhhhhhhhhcccceeeeeeeeechhhhhhhhh

SEQ KGLIDYYLMPSLSTTSRSGKHSFKMKDTHMEDFPKDKETNRDFLPΞGRIESTRTRESPLD SEG

PRD hhhheeeecccccccccccccceeeccccccccccccccccccccccccccccccccccc

SEQ TTNFSKEKDINEFPLCSLPEGVDQEVSKQLPVDIQEEILSGKSREKFQGKGSVΞCPLHAS SEG

PRD cccccccccccccccccccchhhhhhhhhhhhhhhhhhhcccceeeeecccccccchhhh

SEQ RGVLSFFSKKQMQDIPINPRDHLSSSKQVSSVSPCEPGTSGFNSSSSSYMSSQKDYSYYL

SEG xxxxxxxxxx xxxxxxxxxxxxxxxxxxxx .

PRD hcccccccccccccccccccccccccccccccccccccccccccccccccccccchhhhh

SEQ DNRLKDERIΞQGPKEPQGFHFTNSNPAVSAFHSFPNLQSEQLFSRNHTTDΞHKQTVATDS SEG

PRD hhhhhhhhhhcccccccceeeeccccceeecccccccchhhhhhhccccccceeeeeecc

SEQ HEGLTENREPDSVDEKITFPSDIDPQVFYELPEAVQKELLAEWKRTGSDFHIGHK SEG

PRD ccccccccccccccccccccccccceeehhhhhhhhhhhhhhhhhcccccccccc w w w cQ cn ω cn w cn w cn tn cn ω tn cn co ω cn tΛ w w cn w cn cn cn w w o o OoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoooooooooo o OoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOo^ooooooooo

OOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO'O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O hh--^»>000000000000OOOOO0OOOOOOO0OOOO<O 000 O 0 O O 0 O O O 0 O O O O 0 O O O 00 O O 000 O 0 O O O O O '^{— —} _ iii^? o∞o ccoo oαDD ccoo o∞o cαoϊ -^-j ~^-4 θo <_rι crt ccτrιt σσt cmrι ιc^ cΛ σ C^

π lJi l.π Λ Λ μ μ μ μ

DKFZphfbr2_72dl3

group: brain derived

DKFZphfbr2_72dl3 encodes a novel 165 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . unknown seems to be testis specific 9 of 10 EST hits are from testis l brarys

Sequenced by LMU

Locus : unknown

Insert length: 723 bp

Poly A stretch at pos. 704, no polyadenylation signal found

1 AGGGGGGGTA TGGGGGAGGG GGAGACTCTG CAGGAGCCTA ATTCCCCACT 51 CTGAGCTCAC CCTTCTGTCT GCCCGGGCCC TACCCCTTCC CCTACTCTCA 101 CCCTTATAAT CCTTTTCAGC ACTAGGTCTT CCCGTCACCT CCACCTCTCT 151 CCATGACCCG GCTCTGCTTA CCCAGACCCG AAGCACGTGA GGATCCGATC 201 CCAGTTCCTC CAAGGGGCCT GGGTGCTGGG GAGGGGTCAG GTAGTCCAGT 251 GCGTCCACCT GTATCCACCT GGGGCCCTAG CTGGGCCCAG CTCCTGGACA 301 GTGTCCTATG GCTGGGGGCA CTAGGACTGA CAATCCAGGC AGTCTTTTCC 351 ACCACTGGCC CAGCCCTGCT GCTGCTTCTG GTCAGCTTCC TCACCTTTGA 401 CCTGCTCCAT AGGCCCGCAG GTCACACTCT GCCACAGCGC AAACTTCTCA 451 CCAGGGGCCA GAGTCAGGGG GCCGGTGAAG GTCCTGGACA GCAGGAGGCT 501 CTACTCCTGC AAATGGGTAC AGTCTCAGGA CAACTTAGCC TCCAGGACGC 551 ACTGCTGCTG CTGCTCATGG GGCTGGGCCC GCTCCTGAGA GCCTGTGGCA 601 TGCCCTTGAC CCTGCTTGGC CTGGCTTTCT GCCTCCATCC TTGGGCCTGA 651 GAGCCCCTCC CCACAACTCA GTGTCCTTCA AATATACAAT GACCACCCTT 701 CTTCAAAAAA AAAAAAAAAA AAC

BLAST Results

Entry HS860F19 from database EMBLNEW:

Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone 860F19

Score = 2059, P = l.le-85, identities = 423/434

2 exons

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 153 bp to 647 bp; peptide length: 165 Category: putative protein Classification: no clue

1 MTRLCLPRPE AREDPIPVPP RGLGAGEGSG SPVRPPVSTW GPSWAQLLDS

51 VLWLGALGLT IQAVFSTTGP ALLLLLVSFL TFDLLHRPAG HTLPQRKLLT

101 RGQSQGAGEG PGQQEALLLQ MGTVSGQLSL QDALLLLLMG LGPLLRACGM

151 PLTLLGLAFC LHPWA

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_72dl3, frame 3 No Alert BLASTP hits found Pedant information for DKFZphfbr2_72dl3 , frame 3

Report for DKFZphfbr2_72dl3 . 3

[ LENGTH ] 165

[MW ] 17393 . 73

[pi ] 7 . 80

[BLOCKS] BL00068A Malate dehydrogenase proteins

[KW] TRANSMEMBRANE 2

[KW] LOW COMPLEXITY 29.70 %

SEQ MTRLCLPRPEAREDPIPVPPRGLGAGEGSGSPVRPPVSTWGPSWAQLLDSVLWLGALGLT SEG PRD ccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhcccccc MEM

SEQ IQAVFSTTGPALLLLLVSFLTFDLLHRPAGHTLPQRKLLTRGQSQGAGEGPGQQEALLLQ SEG xxxxxxxxxxxxxx xxxxxxxxxxxxxxx .... PRD eeeecccccchhhhhhhhhhhhhhccccccccccccccccccccccccccccchhhhhhh

MEM MMMMMMMMMMMMMMMMM

SEQ MGTVSGQLSLQDALLLLLMGLGPLLRACGMPLTLLGLAFCLHPWA SEG xxxxxxxxxxxxxxxxxxxx PRD hcccccchhhhhhhhhhhhccchhhhhcccccchhhhhhhccccc MEM MMMMMMMMMMMMMMMMM

(No Prosite data available for DKFZphfbr2_72dl3.3) (No Pfam data available for DKFZphfbr2 72dl3.3)

DKFZphfbr2_72112

group: nucleic acid management

Summary DKFZphfbr2_72112 encodes a novel 344 amino acid protein with similarity to YDR126w and other S. cerevisiae proteins.

The novel protein contains a myc-type, helix-loop-helix dimerization domain signature. This helix-loop-helix domain mediates protein dimerization and has been found in proteins such as the myc family of cellular oncogenes, proteins involved in myogenesis and vertebrate proteins that bind specific DNA sequences in various lmmunoglobulin chains enhancers. Therefore, the protein could be a novel DNA-bindmg protein.

The new protein can application in modulating gene expression. similarity to YDR126w ; membrane regions: 2 similarity to YDR126w complete cDNA complete eds, EST hits

Sequenced by LMU

Locus: unknown

Insert length: 1270 bp

Poly A stretch at pos. 1251, no polyadenylation signal found

1 GGGGGCGCCC GGGAGGCGCC GGAGCCCAGC GGCTGGCGCC AGATCCAGGC 51 TCCTGGAAGA ACCATGTCCG GCAGCTACTG GTCATGCCAG GCACACACTG

101 CTGCCCAAGA GGAGCTGCTG TTTGAATTAT CTGTGAATGT TGGGAAGAGG

151 AATGCCAGAG CTGCCGGCTG AAAATTACCC AACCAAGAGA AATCTGCAGG

201 ATGGACTTTC TGGTCCTCTT CTTGTTCTAC CTGGCTTCGG TGCTGATGGG

251 TCTTGTTCTT ATCTGCGTCT GCTCGAAAAC CCATAGCTTG AAAGGCCTGG

301 CCAGGGGAGG AGCACAGATA TTTTCCTGTA TAATTCCAGA ATGTCTTCAG

351 AGAGCCGTGC ATGGATTGCT TCATTACCTT TTCCATACGA GAAACCACAC

401 CTTCATTGTC CTGCACCTGG TCTTGCAAGG GATGGTTTAT ACTGAGTACA

451 CCTGGGAAGT ATTTGGCTAC TGTCAGGAGC TGGAGTTGTC CTTGCATTAC

501 CTTCTTCTGC CCTATCTGCT GCTAGGTGTA AACCTGTTTT TTTTCACCCT

551 GACTTGTGGA ACCAATCCTG GCATTATAAC AAAAGCAAAT GAATTATTAT

601 TTCTTCATGT TTATGAATTT GATGAAGTGA TGTTTCCAAA GAACGTGAGG

651 TGCTCTACTT GTGATTTAAG GAAACCAGCT CGATCCAAGC ACTGCAGTGT

701 GTGTAACTGG TGTGTGCACC GTTTCGACCA TCACTGTGTT TGGGTGAACA

751 ACTGCATCGG GGCCTGGAAC ATCAGGTACT TCCTCATCTA CGTCTTGACC

801 TTGACGGCCT CGGCTGCCAC CGTCGCCATT GTGAGCACCA CTTTTCTGGT

851 CCACTTGGTG GTGATGTCAG ATTTATACCA GGAGACTTAC ATCGATGACC

901 TTGGACACCT CCATGTTATG GACACGGTCA TTCTTATTCA GTACCTGTTC

951 CTGACTTTTC CACGGATTGT CTTCATGCTG GGCTTTGTCG TGGTCCTGAG 1001 CTTCCTCCTG GGTGGCTACC TGTTGTCTGT CCTGTATCTG GCGGCCACCA 1051 ACCAGACTAC TAACGAGTGG TACAGAGGTG TCTGGGCCTG GTGCCAGCGT 1101 TGTCCCCTTG TGGCCTGGCC TCCGTCAGCA GAGCCCCAAG TCCACCGGAA 1151 CATTCACTCC CATGGGCTTC GGAGCAACCT TCAAGAGATC TTTCTACCTG 1201 CCTTTCCATG TCATGAGAGG AAGAAACAAG AATGACAAGT GTATGACTGC 1251 CAAAAAAAAA AAAAAAAAAC

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 201 bp to 1232 bp; peptide length: 344 Category: similarity to unknown protein 1 MDFLVLFLFY LASVLMGLVL ICVCSKTHSL KGLARGGAQI FSCIIPECLQ

51 RAVHGLLHYL FHTRNHTFIV LHLVLQGMVY TEYTWEVFGY CQELELSLHY

101 LLLPYLLLGV NLFFFTLTCG TNPGIITKAN ELLFLHVYEF DEVMFPKNVR

151 CSTCDLRKPA RSKHCSVCNW CVHRFDHHCV WVNNCIGAWN IRYFLIYVLT

201 LTASAATVAI VSTTFLVHLV VMSDLYQETY IDDLGHLHVM DTVILIQYLF

251 LTFPRIVFML GFVVVLSFLL GGYLLSVLYL AATNQTTNEW YRGVWAWCQR

301 CPLVAWPPSA EPQVHRNIHS HGLRSNLQEI FLPAFPCHER KKQE

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_72112, frame 3

TREMBL :SPBC13G1_7 gene: "SPBC13G1.07"; product: "hypothetical protein"; S. pombe chromosome II cosmid cl3Gl., N = 2, Score = 247, P = 1.4e-22

TREMBL:CED2021_3 gene: "D2021.2"; Caenorhabditis elegans cosmid D2021., N = 1, Score = 209, P = 9e-17

TREMBL :CEC43H6_2 gene: "C43H6.7"; Caenorhabditis elegans cosmid C43H6., N = 1, Score = 206, P = 5.2e-15

PIR:S52691 probable membrane protein YDR126w - yeast (Saccharomyces cerevisiae), N = 1, Score = 207, P = 8.4e-15

PIR:E71607 metal binding protein (DHHC domain) PFB0725C - malaria parasite (Plasmodium falciparum), N = 1, Score = 182, P = l.le-13

>TREMBL:SPBC13G1_7 gene: "SPBC13G1.07"; product: "hypothetical protein"; S. pombe chromosome II cosmid cl3Gl. Length = 356

HSPs:

Score = 247 (37.1 bits), Expect = 1.4e-22, Sum P(2) = 1.4e-22 Identities = 55/148 (37%), Positives = 85/148 (57%)

Query: 52 AVHGLLHYLFHTRNH--TFIVLHLVLQGM VYTEYTWEVFGYCQELELSLHYLLLPY 105

A+ L +Y+ + N F+ L L+ G+ +Y + F + + L +LLPY Sbjct: 64 AMRSLSNYVLYKNNPLVVFLYLALITIGIASFFIYGSΞLTQKFSIIDWISV-LTSVLLPY 122

Query: 106 LLLGVNLFFFTLTCGTNPGIITKANELLFLHVYEFD-EVMFPKNVRCSTCDLRKPARSKH 164

++L+ + +NPG I N + +D ++ FP +CSTC KPARSKH Sbjct: 123 ISLY IAAKSNPGKIDLKNWNEASRRFPYDYKIFFPN—KCSTCKFEKPARSKH 173

Query: 165 CSVCNWCVHRFDHHCVWVNNCIGAWNIRYFLIYVL 199

C +CN CV +FDHHC+W+NNC+G N RYF +++L Sbjct: 174 CRLCNICVEKFDHHCIWINNCVGLNNARYFFLFLL 208

Score = 43 (6.5 bits), Expect = 1.4e-22, Sum P(2) = 1.4e-22 Identities = 10/35 (28%), Positives = 17/35 (48%)

Query: 257 VFMLGFVV-VLSFLLGGYLLSVLYLAATNQTTNEW 290

VF++ + VL L GY ++Y T + +W Sbjct: 254 VFLISLICSVLVLCLLGYEFFLVYAGYTTNESEKW 288

Pedant information for DKFZphfbr2_72112, frame 3

Report for DKFZphfbr2_72112.3

[LENGTH] 344

[MW] 39677.23

[pi] 7.26

[HOMOL] TREMBL :SPBC13G1_7 gene: "SPBC13G1.07"; product: "hypothetical protein"; S. pombe chromosome II cosmid C13G1. 3e-17

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YDR126w] le-16

[FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins

[S. cerevisiae, YDR264c] 8e-05

[FUNCAT] 10.05.99 other pheromone response activities [S. cerevisiae, YDR264C] 8e-05

[PIRKW] transmembrane protein 4e-15

[SUPFAM] ankyrin repeat homology le-10

[SUPFAM] unassigned ankyrin repeat proteins le-10

[PROSITE] MYRISTYL 4

[PROSITE] CK2_PHOSPHO_SITE 3 [PROSITE] PKC_PHOSPHO_SITE 1

[PROSITE] ASN_GLYCOSYLATION 2

[KW] SIGNAL_PEPTIDE 30

[KW] TRANSMEMBRANE 2

[KW] LOW COMPLEXITY 16.57

SEQ MDFLVLFLFYLASVLMGLVLICVCSKTHSLKGLARGGAQIFSCIIPECLQRAVHGLLHYL

SEG

PRD ccchhhhhhhhhhhhhhheeeeeeccccceeeeecccceeeeeeehhhhhhhhhhhheee

MEM

SEQ FHTRNHTFIVLHLVLQGMVYTEYTWEVFGYCQELELSLHYLLLPYLLLGVNLFFFTLTCG

SEG xxxxxxxxxxxxxxxxxxx

PRD ecccchhhhhhhhhhccchhhhhhhheeeeccceeehhhhhhhhhhhhhhcccceeeecc

MEM MMMMMMMMMMMMMMMMMMMMMMMMM

SEQ TNPGIITKANELLFLHVYEFDEVMFPKNVRCSTCDLRKPARSKHCSVCNWCVHRFDHHCV

SEG

PRD ccccccccccchhhhhhhhhcccccccceeeecccccccccccccccceeeecccccccc

MEM M MMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ WVNNCIGAWNIRYFLIYVLTLTASAATVAIVSTTFLVHLVVMSDLYQETYIDDLGHLHVM

SEG xxxxxxxxxxxxxxxxx

PRD cccccccccccchhhhhhhhhccchhhhhhhhhhhhhhhhhccccccccccccccccchh

MEM

SEQ DTVILIQYLFLTFPRIVFMLGFVVVLΞFLLGGYLLSVLYLAATNQTTNEWYRGVWAWCQR

SEG xxxxxxxxxxxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhccccccccceeecccchhhhhhhhhcccchhhhhhhhhhhcccc

MEM

SEQ CPLVAWPPSAEPQVHRNIHSHGLRSNLQEIFLPAFPCHERKKQE

SEG

PRD cccccccccccccceeecccccccccceeeeecccccccccccc

MEM

Prosite for DKFZphfbr2_72112.3

PS00001 65->69 ASN_GLYCOSYLATION PDOC00001 PS00001 284->288 ASN_GLYCOSYLATION PDOC00001 PS00005 29->32 PKC_PHOSPHO_SITE PDOC00005 PS00006 152->156 CK2_PHOSPHO_SITE PDOC00006 PS00006 229->233 CK2_PHOSPHO_SITE PDOC00006 PS00006 286->290 CK2_PHOSPHO_SI E PDOC00006 PS00008 32->38 MYRISTYL PDOC00008 PS00008 77->83 MYRISTYL PDOC00008 PS00008 120->126 MYRISTYL PDOC00008 PS00008 322->328 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_72112.3)

DKFZphfbr2_72ml6

group: unknown

DKFZphfbr2_72ml6 encodes a novel 287 am o acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . unknown complete cDNA, complete eds, EST hits

Sequenced by LMU

Locus: /map="26.2 cR from top of Chrl6 linkage group"

Insert length: 1462 bp

Poly A stretch at pos. 1441, polyadenylation signal at pos. 1421

1 GGGGAGGACC GGAGGACCGA GGACAGAAAG ATTGGTGGAC AGGAGCAGCG

51 GCCGGTGGGG AGGGCGCTCG GCGGCGGCCT GCGGCCATGG CCACCGTGAT

101 GGCAGCGACG GCGGCGGAGC GGGCGGTGCT GGAGGAGGAG TTCCGCTGGC

151 TGCTGCACGA CGAGGTGCAC GCTGTGTTGA AGCAGCTGCA GGACATCCTC

201 AAGGAGGCCT CTCTGCGCTT CACTCTGCCG GGCTCCGGCA CTGAGGGGCC

251 CGCCAAGCAA GAGAACTTCA TCCTAGGCAG CTGTGGCACA GACCAGGTGA

301 AGGGTGTGCT GACTCTGCAG GGGGATGCCC TCAGCCAGGC GGATGTGAAC

351 CTGAAGATGC CCCGGAACAA CCAGCTGCTG CACTTCGCCT TCCGGGAGGA

401 CAAGCAGTGG AAGCTGCAGC AGATCCAGGA TGCCAGAAAC CATGTGAGCC

451 AAGCCATTTA CCTGCTTACC AGCCGGGACC AGAGCTACCA GTTCAAGACG

501 GGCGCTGAGG TCCTCAAGCT GATGGACGCA GTGATGCTGC AGCTGACCAG

551 AGCCCGAAAC CGGCTCACCA CCCCCGCCAC CCTCACCCTC CCCGAGATCG

601 CCGCCAGCGG CCTCACGCGG ATGTTCGCCC CTGCCCTGCC GTCCGACCTG

651 CTGGTCAACG TCTACATCAA CCTCAACAAG CTCTGCCTCA CGGTGTACCA

701 GCTGCATGCC CTGCAGCCCA ACTCCACCAA GAACTTCCGC CCAGCTGGGG

751 GCGCGGTGCT GCATAGCCCT GGGGCCATGT TCGAGTGGGG CTCTCAGCGC

801 CTGGAGGTGA GCCACGTGCA CAAAGTGGAG TGCGTGATCC CCTGGCTCAA

851 CGACGCCCTG GTCTACTTCA CCGTCTCCCT GCAGCTCTGC CAGCAGCTTA

901 AGGACAAGAT CTCCGTGTTC TCCAGCTACT GGAGCTACAG ACCCTTCTGA

951 TCACAGCACC CAGGAGCTTG TCTCCAGGAA GGCGGCCCCG TCCCCTACTC

1001 ATACCCACCA CAGAGCACCA GCCAGTGCCA ACGCCAGGCT GCTATTTATC

1051 TCCCTATCCC ACCCCCTACC CCACCTAACA CATTTGCACT GCCGGGAATG

1101 GACACTGGAA GTGCCAGGAG GAAGGAAGGC TGGTTTGGTG GGGTAGTGGG

1151 GAGGTCAGGG AGGCGGGGCC AAGGGTGTCC CACATTCCCA ACACCGCCCT

1201 CTGATCACCA TGGGAATCTT TGGACTCAGG ACAGGGCCAG GCGCAGGGCT

1251 CTCCCTCCTC TCCCCTTCGC TGTCCCCTCC CCCTGGAGGG CATGGTGTCG

1301 GGGGGTGGCA CTGAGCTATG AGTCCCGGGG ATGGTGAGGA ACGCCACAGA

1351 CAGAGCCACC CTAGGAGTGA GTATAGTGCT GGTGACTGTG TTTCATAGCC

1401 CCAGTCCAGG GCTGTCTAAG AAATAAAGAT CATCAGACTC CAAAAAAAAA

1451 AAAAAAAAAA AC

BLAST Results

Entry HS604351 from database EMBL: human STS WI-18474. Score = 1178, P = 1.5e-48, identities = 250/268

Medline entries

No Medlme entry

Peptide information for frame 3

ORF from 87 bp to 947 bp; peptide length: 287 Category: similarity to unknown protein 1 MATVMAATAA ERAVLEEEFR WLLHDEVHAV LKQLQDILKE ASLRFTLPGS

51 GTEGPAKQEN FILGSCGTDQ VKGVLTLQGD ALSQADVNLK MPRNNQLLHF

101 AFREDKQWKL QQIQDARNHV SQAIYLLTSR DQSYQFKTGA EVLKLMDAVM

151 LQLTRARNRL TTPATLTLPE IAASGLTRMF APALPSDLLV NVYINLNKLC

201 LTVYQLHALQ PNSTKNFRPA GGAVLHSPGA MFEWGSQRLE VSHVHKVECV

251 IPWLNDALVY FTVSLQLCQQ LKDKISVFSS YWSYRPF

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_72ml6, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphfbr2_72ml6, frame 3

Report for DKFZphfbr2_72ml6.3

[LENGTH] 287

[MW] 32254.40

[pi] 8.30

[HOMOL] TREMBL:AF025459_2 gene: "H14A12.3"; Caenorhabditis elegans cosmid H14A12. 3e-14

[PROSITE] MYRISTYL 1

[PROSITE] CK2_PHOSPHO_SITE 6

[PROSITE] PKC_PHOSPHO_SI E 5

[PROSITE] ASN_GLYCOSYLATION 1

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 6.27 '

SEQ MATVMAATAAERAVLEEEFRWLLHDEVHAVLKQLQDILKEASLRFTLPGSGTEGPAKQEN SEG xxxxxxxxxxxxxxxxxx PRD ccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccchhhh

SEQ FILGSCGTDQVKGVLTLQGDALSQADVNLKMPRNNQLLHFAFREDKQWKLQQIQDARNHV

SEG PRD hhccccccceeeeeeeeccccchhhhhhhcccccchhhhhhhhhchhhhhhhhhhhhchh

SEQ SQAIYLLTSRDQSYQFKTGAEVLKLMDAVMLQLTRARNRLTTPATLTLPEIAASGLTRMF SEG PRD hhhhhhhhccccceeecchhhhhhhhhhhhhhhhhhhhhhcccccccccccccccccccc

SEQ APALPSDLLVNVYINLNKLCLTVYQLHALQPNSTKNFRPAGGAVLHSPGAMFEWGSQRLE SEG PRD cccccccceeeeehhhhhhhhhhheeeecccccccccccccceeecccccccccccccee

SEQ VSHVHKVECVIPWLNDALVYFTVSLQLCQQLKDKISVFSSYWSYRPF SEG PRD eeeeeeeeeeeecccceeeeeeehhhhhhhhhhhhheeeeeeeeccc

Prosite for DKFZphfbr2_72ml6.3

PS00001 212->216 ASN_GLYCOSYLATION PDOC00001 PS00005 42->45 PKC_PHOSPHO_SITE PDOC00005 PS00005 128->131 PKC_PHOSPHO_SITE PDOC00005 PS00005 213->216 PKC_PHOSPHO_SITE PDOC00005 PS00005 236->239 PKC_PHOSPHO_SITE PDOC00005 PS00005 283->286 PKC_PHOSPHO_SITE PDOC00005 PS00006 8->12 CK2_PHOSPHO_SITE PDOC00006 PS00006 50->54 CK2_PHOSPHO_SITE PDOC00006 PS00006 83->87 CK2_PHOΞPHO_SITE PDOC00006 PS00006 128->132 CK2_PHOSPHO_SITE PDOC00006 PS00006 138->142 CK2_PHOSPHO_SITE PDOC00006 PS00006 167-M71 CK2_PHOSPHO_SITE PDOC00006 PS00008 64->70 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_72ml6.3) DKFZphfbr2_72nl2

group: brain derived

DKFZphfbr2_72nl2 encodes a novel 117 amino acid protein with similarity to a protein with conserved sequence in bacteria and eukaπota.

The novel protein is very similar to human MM46, human and rat gangliosiode expression factor- 2 (GEF2), C. elegans 14.8 kD protein C32D5.9 and Laccaria bicolor symbiosis-related protein LBU93506_1. The function of this highly conserved proteins is not known.

The new protein can find application in studying the expression profile of brain-specific genes . strong similarity to rat GANGLIOSIDE EXPRESSION FACTOR 2 (GEF-2) complete cDNA, complete eds, EST hits

Sequenced by LMU

Locus: /map="12"

Insert length: 1880 bp

Poly A stretch at pos. 1859, polyadenylation signal at pos. 1830

1 GGGGGCCGGT ATTTCTCCAT CTGGCTCTCC TCTACCTCCA GGCAGGCTCA

51 CCCGAGATCC CCGCCCCGAA CCCCCCCTGC ACACTCGGCC CAGCGCTGTT

101 GCCCCCGGAG CGGACGTTTC TGCAGCTATT CTGAGCACAC CTTGACGTCG

151 GCTGAGGGAG CGGGACAGGG TCAGCGGCGA AGGAGGCAGG CCCCGCGCGG

201 GGATCTCGGA AGCCCTGCGG TGCATCATGA AGTTCCAGTA CAAGGAGGAC

251 CATCCCTTTG AGTATCGGAA AAAGGAAGGA GAAAAGATCC GGAAGAAATA

301 TCCGGACAGG GTCCCCGTGA TTGTAGAGAA GGCTCCAAAA GCCAGGGTGC

351 CTGATCTGGA CAAGAGGAAG TACCTAGTGC CCTCTGACCT TACTGTTGGC

401 CAGTTCTACT TCTTAATCCG GAAGAGAATC CACCTGAGAC CTGAGGACGC

451 CTTATTCTTC TTTGTCAACA ACACCATCCC TCCCACCAGT GCTACCATGG

501 GCCAACTGTA TGAGGACAAT CATGAGGAAG ACTATTTTCT GTATGTGGCC

551 TACAGTGATG AGAGTGTCTA TGGGAAATGA GTGGTTGGAA GCCCAGCAGA

601 TGGGAGCACC TGGACTTGGG GGTAGGGGAG GGGTGTGTGT GCGCGACATG

651 GGGAAAGAGG GTGGCTCCCA CCGCAAGGAG ACAGAAGGTG AAGACATCTA

701 GAAACATTAC ACCACACACA CCGTCATCAC ATTTTCACAT GCTCAATTGA

751 TATTTTTTGC TGCTTCCTCG GCCCAGGGAG AAAGCATGTC AGGACAGAGC

801 TGTTGGATTG GCTTTGATAG AGGAATGGGG ATGATGTAAG TTTACAGTAT

851 TCCTGGGGTT TAATTGTTGT GCAGTTTCAT AGATGGGTCA GGAGGTGGAC

901 AAGTTGGGGC CAGAGATGAT GGCAGTCCAG CAGCAACTCC CTGTGCTCCC

951 TTCTCTTTGG GCAGAGATTC TATTTTTGAC ATTTGCACAA GACAGGTAGG

1001 GAAAGGGGAC TTGTGGTAGT GGACCATACC TGGGGACCAA AAGAGACCCA

1051 CTGTAATTGA TGCATTGTGG CCCCTGATCT TCCCTGTCTC ACACTTCTTT

1101 TCTCCCATCC CGGTTGCAAT CTCACTCAGA CATCACAGTA CCACCCCAGG

1151 GGTGGCAGTA GACAACAACC CAGAAATTTA GACAGGGATC TCTTACCTTT

1201 GGAAAATAGG GGTTAGGCAT GAAGGTGGTT GTGATTAAGA AGATGGTTTT

1251 GTTATTAAAT AGCATTAAAC TGGAATTGAC AAGAGTGTTG AGCATCCCTG

1301 TCTAACCTGC TCTTTCTCTT TGGTGCCCCT TATCTCACCC CTTCCTTGGA

1351 ATTTAATAAG TCTCAGGCAT TTCCAATTGT AGACTAAAAC CACTCTTAGC

1401 ATCTCCTCTA GTATTTTCCA TGTATCAGGA AAGAGGTGTC TTATGTAGGG

1451 AGGGGGCAAG TATGAAGTAA GGTAATTATA TACTACTCTC ATTCAGGATT

1501 CTTGCTCCCA TGCTGCTGTC CCTTCAGGCT CACATGCACA GGAATGCTAC

1551 ATGATGGCCA GCTGCTTCCC TCCTTGGTTA TCATCCACTG CAGCTGCTAG

1601 TTAGAAAGGT TTGGAGGGAT GACTTTTAGT AAATCATGGG GATTTTATTG

1651 ATTTATTTTC ACTTTTGGGA TTTTGTGGGG TGGGAGTGGG GAGCAGGAAT

1701 TGCACTCAGA CATGACATTT CAATTCATCT CTGCTAATGA AAAGGGTTCT

1751 TTCTCTTGGG GGAAATGTGT GTGTCAGTTC TGTCAGCTGC AAGTTCTTGT

1801 ATAATGAAGT CAATGCCATC AGGCCAAGGA AATAAAATAA TTGCTTACCT

1851 TAAAAATCGA AAAAAAAAAA AAAAAAAAAC

BLAST Results

Entry HS418210 from database EMBL: human STS SHGC-10496. Score = 1916, P = 4.0e-80, identities = 394/400

Entry AC006514 from database EMBLNEW:

*** SEQUENCING IN PROGRESS *** Homo sapiens; HTGS phase 1, 68 unordered pieces .

Score = 610 , P = 2 .7e-16, identities = 128 /134 4 exons Medl e entries

No Medlme entry

Peptide information for frame 2

ORF from 227 bp to 577 bp; peptide length: 117 Category: strong similarity to known protein

1 MKFQYKEDHP FEYRKKEGEK IRKKYPDRVP VIVEKAPKAR VPDLDKRKYL 51 VPSDLTVGQF YFLIRKRIHL RPEDALFFFV NNTIPPTSAT MGQLYEDNHE 101 EDYFLYVAYS DESVYGK

BLASTP hits

Entry YQD9_CAEEL from database SWISSPROT:

HYPOTHETICAL 14.8 KD PROTEIN C32D5.9 IN CHROMOSOME II.

Score = 496, P = 1.8e-47, identities = 91/116, positives = 105/116

Entry SYRP_LACBI from database SWISSPROT:

SYMBIOSIS-RELATED PROTEIN.

Score = 390, P = 3.1e-36, identities = 68/117, positives = 94/117

Entry LBU93506_1 from database TREMBL: product: "symbiosis-related protein"; Laccaria bicolor symbiosis-related protein mRNA, partial eds.

Score = 390, P = 3.1e-36, identities = 68/117, positives = 94/117

Entry GEF2_RAT from database SWISSPROT:

GANGLIOSIDE EXPRESSION FACTOR 2 (GEF-2) .

Score = 373, P = 2.0e-34, identities = 71/116, positives = 88/116

Alert BLASTP hits for DKFZphfbr2_72nl2, frame 2

TREMBLNEW:AF044671_1 product: "MM46"; Homo sapiens MM46 mRNA, complete eds., N = 1, Score = 549, P = 4.7e-53

SWISSPROT:GEF2_HUMAN GANGLIOSIDE EXPRESSION FACTOR 2 (GEF-2)., N = 1, Score = 373, P = 2.1e-34

>TREMBLNEW:AF044671_1 product: "MM46"; Homo sapiens MM46 mRNA, complete eds.

Length = 117

HSPs:

Score = 549 (82.4 bits), Expect = 4.7e-53, P = 4.7e-53 Identities = 101/116 (87%), Positives = 110/116 (94%)

Query: 1 MKFQYKEDHPFEYRKKEGEKIRKKYPDRVPVIVEKAPKARVPDLDKRKYLVPSDLTVGQF 60

MKF YKE+HPFE R+ EGEKIRKKYPDRVPVIVEKAPKAR+ DLDK+KYLVPSDLTVGQF Sbjct: 1 MKFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQF 60

Query: 61 YFLIRKRIHLRPEDALFFFVNNTIPPTSATMGQLYEDNHEEDYFLYVAYSDEΞVYG 116

YFLIRKRIHLR EDALFFFVNN IPPTSATMGQLY+++HEED+FLY+AYSDESVYG Sbjct: 61 YFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEHHEEDFFLYIAYSDESVYG 116

Pedant information for DKFZphfbr2_72nl2, frame 2

Report for DKFZphfbr2_72nl2.2

[LENGTH] 117 [MW] 14044.07 [pi] 8.67 [HOMOL] TREMBL:AF044671_1 product: "MM46"; Homo sapiens MM46 mRNA, complete eds. le-56 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YBL078c] 4e-36

[FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YBL078c] 4e-36

[FUNCAT] 06.13.04 lysosomal and vacuolar degradation [S. cerevisiae, YBL078c] 4e-36

[SUPFAM] hypothetical protein YBL078C 8e-35

[PROSITE] ASN_GLYCOSYLATION 1

[KW] Alpha_Beta

SEQ MKFQYKEDHPFEYRKKEGEKIRKKYPDRVPVIVEKAPKARVPDLDKRKYLVPSDLTVGQF PRD cccccccccchhhhhhhhhhhhhhccccceeeeeccccccccccccceeecccccchhhh

SEQ YFLIRKRIHLRPEDALFFFVNNTIPPTSATMGQLYEDNHEEDYFLYVAYSDESVYGK PRD hhhhhhhhhhccccceeeeecccccccchhhhhhhhhccccceeeeeeecccccccc

Prosite for DKFZphfbr2_72nl2.2 PS00001 81->85 ASN_GLYCOSYLATION PDOC00001

(No Pfam data available for DKFZphfbr2_72nl2.2)

DKFZphfbr2_78c24

group: signal transduction

DKFZphfbr2_78c24 encodes a novel 563 ammo acid protein with strong similarity to guanylate- bindmg proteins (GBPs) .

GBPs were originally described as proteins that are strongly induced by mterferons and are capable of binding to agarose-immobilized guanine nucleotides. hGBPl, the first of two members of this protein family in humans, represents a novel type of GTPase. The novel protein contains an ATP/GTP-binding site motif A (P-loop) and a RGD cell attachment site. It seems to be a new member of the GBP-family and shows a splicing pattern not described previously.

The new protein can find application in modulating/blocking the response of cells to mterferons . strong similarity to guanine nucleotide-binding protein 1/2 but different "splice variant" aa 211-245 of GBP1/2 missing

Sequenced by MediGenomix

Locus: unknown

Insert length: 2952 bp

Poly A stretch at pos. 2927, polyadenylation signal at pos. 2914

1 CAGTTTCATT AGGCTCTGAA GCCATTACAA AGGTTGCTTA ACTTCTAATT 51 ATTTGATCAC TGAGGAAAAT CCAGAAAGCT ACACAACACT GAAGGGGTGA

101 AATAAAAGTC CAGCGATCCA GCGAAAGAAA AGAGAAGTGA CAGAAACAAC

151 TTTACCTGGA CTGAAGATAA AAGCACAGAC AAGAGAACAA TGCCCTGGAC

201 ATGGCTCCAG AGATCCACAT GACAGGCCCA ATGTGCCTCA TTGAGAACAC

251 TAATGGGGAA CTGGTGGCGA ATCCAGAAGC TCTGAAAATC CTGTCTGCCA

301 TTACACAGCC TGTGGTGGTG GTGGCAATTG TGGGCCTCTA CCGCACAGGA

351 AAATCCTACC TGATGAACAA GCTAGCTGGG AAGAATAAGG GCTTCTCTCT

401 GGGCTCCACA GTGAAATCTC ACACCAAAGG AATCTGGATG TGGTGTGTGC

451 CTCACCCCAA AAAGCCAGAA CACACCTTAG TCCTGCTTGA CACTGAGGGC

501 CTGGGAGATG TAAAGAAGGG TGACAACCAG AATGACTCCT GGATCTTCAC

551 CCTGGCCGTC CTCCTGAGCA GCACTCTCGT GTACAATAGC ATGGGAACCA

601 TCAACCAGCA GGCTATGGAC CAACTGTACT ATGTGACAGA GCTGACACAT

651 CGAATCCGAT CAAAATCCTC ACCTGATGAG AATGAGAATG AGGATTCAGC

701 TGACTTTGTG AGCTTCTTCC CAGATTTTGT GTGGACACTG AGAGATTTCT

751 CCCTGGACTT GGAAGCAGAT GGACAACCCC TCACACCAGA TGAGTACCTG

801 GAGTATTCCC TGAAGCTAAC GCAAGGTAAC AGGAAGCTTG CCCAGCTTGA

851 GAAACTACAA GATGAAGAGC TGGACCCTGA ATTTGTGCAA CAAGTAGCAG

901 ACTTCTGTTC CTACATCTTT AGCAATTCCA AAACTAAAAC TCTTTCAGGA

951 GGCATCAAGG TCAATGGGCC TTGTCTAGAG AGCCTAGTGC TGACCTATAT 1001 CAATGCTATC AGCAGAGGGG ATCTGCCCTG CATGGAGAAC GCAGTCCTGG 1051 CCTTGGCCCA GATAGAGAAC TCAGCCGCAG TGCAAAAGGC TATTGCCCAC 1101 TATGACCAGC AGATGGGCCA GAAGGTGCAG CTGCCCGCAG AAACCCTCCA 1151 GGAGCTGCTG GACCTGCACA GGGTTAGTGA GAGGGAGGCC ACTGAAGTCT 1201 ATATGAAGAA CTCTTTCAAG GATGTGGACC ATCTGTTTCA AAAGAAATTA 1251 GCGGCCCAGC TAGACAAAAA GCGGGATGAC TTTTGTAAAC AGAATCAAGA 1301 AGCATCATCA GATCGTTGCT CAGCTTTACT TCAGGTCATT TTCAGTCCTC 1351 TAGAAGAAGA AGTGAAGGCG GGAATTTATT CGAAACCAGG GGGCTATTGT 1401 CTCTTTATTC AGAAGCTACA AGACCTGGAG AAAAAGTACT ATGAGGAACC 1451 AAGGAAGGGG ATACAGGCTG AAGAGATTCT GCAGACATAC TTGAAATCCA 1501 AGGAGTCTGT GACCGATGCA ATTCTACAGA CAGACCAGAT TCTCACAGAA 1551 AAGGAAAAGG AGATTGAAGT GGAATGTGTA AAAGCTGAAT CTGCACAGGC 1601 TTCAGCAAAA ATGGTGGAGG AAATGCAAAT AAAGTATCAG CAGATGATGG 1651 AAGAGAAAGA GAAGAGTTAT CAAGAACATG TGAAACAATT GACTGAGAAG 1701 ATGGAGAGGG AGAGGGCCCA GTTGCTGGAA GAGCAAGAGA AGACCCTCAC 1751 TAGTAAACTT CAGGAACAGG CCCGAGTACT AAAGGAGAGA TGCCAAGGTG 1801 AAAGTACCCA ACTTCAAAAT GAGATACAAA AGCTACAGAA GACCCTGAAA 1851 AAAAAAACCA AGAGATATAT GTCGCATAAG CTAAAGATCT AAACAACAGA 1901 GCTTTTCTGT CATCCTAACC CAAGGCATAA CTGAAACAAT TTTAGAATTT 1951 GGAACAAGTG TCACTATATT TGATAATAAT TAGATCTTGC ATCATAACAC 2001 TAAAAGTTTA CAAGAACATG CAGTTCAATG ATCAAAATCA TGTTTTTTCC 2051 TTAAAAAGAT TGTAAATTGT GCAACAAAGA TGCATTTACC TCTGTACCAA 2101 CAGAGGAGGG ATCATGAGTT GCCACCACTC AGAAGTTTAT TCTTCCAGAC 2151 GACCAGTGGA TACTGAGGAA AGTCTTAGGT AAAAATCTTG GGACATATTT 2201 GGGCACTGGT TTGGCCAAGT GTACAATAGG TCCCAATATC AGAAACAACC 2251 ATCCTAGCTT CCTAGGGAAG ACAGTGTACA GTTCTCCATT ATATCAAGGC 2301 TACAAGGTCT ATGAGCAATA ATGTGATTTC TGGACATTGC CCATGGATAA 2351 TTCTCACTGA TGGATCTCAA GCTAAAGCAA ACCATCTTAT ACAGAGATCT 2401 AGAATCTTAT ATTTTCCATA GGAAGGTAAA GAAATCATTA GCAAGAGTAG 2451 GAATTGAATC ATAAACAAAT TGGCTAATGA AGAAATCTTT TCTTTCTTGT 2501 TCAATTCATC TAGATTATAA CCTTAATGTG ACACCTGAGA CCTTTAGACA 2551 GTTGACCCTG AATTAAATAG TCACATGGTA ACAATTATGC ACTGTGTAAT

2601 TTTAGTAATG TATAACATGC AATGATGCAC TTTAACTGAA GATAGAGACT

2651 ATGTTAGAAA ATTGAACTAA TTTAATTATT TGATTGTTTT AATCCTAAAG

2701 CATAAGTTAG TCTTTTCCTG ATTCTTAAAG GTCATACTTG AAATCCTGCC

2751 AATTTTCCCC AAAGGGAATA TGGAATTTTT TTTGACTTTC TTTTGAGCAA

2801 TAAAATAATT GTCTTGCCAT TACTTAGTAT ATGTAGACTT CATCCCAATT

2851 GTCAAACATC CTAGGTAAGT GGTTGACATT TCTTACAGCA ATTACAGATT

2901 ATTTTTGAAC TAGAAATAAA CTAAACTAGA AACAAAAAAA AAAAAAAAAA

2951 AA

BLAST Results

No BLAST result

Medline entries

No Medlme entry

Peptide information for frame 3

ORF from 201 bp to 1889 bp; peptide length: 563 Category: strong similarity to known protein Classification: Cell signaling/communication Prosite motifs: RGD (272-275) ATP GTP A (45-53)

1 MAPEIHMTGP MCLIENTNGE LVANPEALKI LSAITQPVVV VAIVGLYRTG 51 KSYLMNKLAG KNKGFSLGST VKSHTKGIWM WCVPHPKKPE HTLVLLDTEG 101 LGDVKKGDNQ NDSWIFTLAV LLΞSTLVYNS MGTINQQAMD QLYYVTELTH 151 RIRSKSSPDE NENEDΞADFV SFFPDFVWTL RDFSLDLEAD GQPLTPDEYL 201 EYSLKLTQGN RKLAQLEKLQ DEELDPEFVQ QVADFCSYIF SNSKTKTLSG 251 GIKVNGPCLE SLVLTYINAI SRGDLPCMEN AVLALAQIEN SAAVQKAIAH 301 YDQQMGQKVQ LPAETLQELL DLHRVSEREA TEVYMKNSFK DVDHLFQKKL 351 AAQLDKKRDD FCKQNQEASS DRCSALLQVI FSPLEEEVKA GIYSKPGGYC 401 LFIQKLQDLE KKYYEEPRKG IQAEEILQTY LKSKESVTDA ILQTDQILTE 451 KEKEIEVECV KAESAQASAK MVEEMQIKYQ QMMEEKEKSY QEHVKQLTEK 501 MERERAQLLE EQEKTLTSKL QEQARVLKER CQGESTQLQN EIQKLQKTLK 551 KKTKRYMSHK LKI

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_78c24, frame 3

PIR:A41268 guanine nucleotide-bindmg protein 1 - human, N = 2, Score = 1306, P = 4.9e-238

PIR:A46459 macrophage-activation gene-1 protein mag-1 - mouse, N = 2, Score = 942, P = 8.9e-184

PIR:S70524 guanine nucleotide-binding protein 2 - human, N = 2, Score = 1131, P = 4.1e-210

TREMBL :AF077007_1 gene: "Gbp2"; product: "lnterferon-mduced guanylate binding protein GBP-2"; Mus musculus lnterferon-induced guanylate binding protein GBP-2 (Gbp2) mRNA, complete eds., N = 2, Score = 904, P = 1.2e-179

>PIR:A41268 guanine nucleotide-bmdmg protein 1 - human Length = 592

HSPs:

Score = 1306 (195.9 bits), Expect = 4.9e-238, Sum P(2) = 4.9e-238 Identities = 264/332 (79%), Positives = 288/332 (86%)

Query: 211 RKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIKVNGPCLESLVLTYINAI 270

RKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGI+VNGP LESLVLTY+NAI Sbjct: 245 RKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIQVNGPRLESLVLTYVNAI 304 Query: 271 SRGDLPCMENAVLALAQIENSAAVQKAIAHYDQQMGQKVQLPAETLQELLDLHRVSEREA 330

S GDLPCMENAVLALAQIENSAAVQKAIAHY+QQMGQKVQLP E+LQELLDLHR SEREA Sb_jct: 305 SSGDLPCMENAVLALAQIENSAAVQKAIAHYEQQMGQKVQLPTESLQELLDLHRDSEREA 364

Query: 331 TEVYMKNSFKDVDHLFQKKLAAQLDKKRDDFCKQNQEASSDRCSALLQVIFSPLEEEVKA 390

EV++++SFKDVDHLFQK+LAAQL+KKRDDFCKQNQEASSDRCS LLQVIFSPLEEEVKA Sbjct: 365 IEVFIRSSFKDVDHLFQKELAAQLEKKRDDFCKQNQEASSDRCSGLLQVIFSPLEEEVKA 424

Query: 391 GIYΞKPGGYCLFIQKLQDLEKKYYEEPRKGIQAEEILQTYLKSKESVTDAILQTDQILTX 450

GIYSKPGGY LF+QKLQDL+KKYYEEPRKGIQAEEILQTYLKSKES+TDAILQTDQ LT Sbjct: 425 GIYSKPGGYRLFVQKLQDLKKKYYEEPRKGIQAEEILQTYLKSKESMTDAILQTDQTLTE 484

Query: 451 XXXXXXXXXXXXXSAQASAKMVEEMQIKYQQMMEEKEKSYQEHVKQLTEKMXXXXXXXXX 510

SAQASAKM++EMQ K +QMME+KE+SYQEH+KQLTEKM Sbjct: 485 KEKEIEVERVKAESAQASAKMLQEMQRKNEQMMEQKERSYQEHLKQLTEKMENDRVQLLK 544

Query: 511 XXXKTLTSKLQEQARVLKERCQGESTQLQNEI 542

+TL KLQEQ ++LKE Q ES ++NEI Sbjct: 545 EQERTLALKLQEQEQLLKEGFQKESRIMKNEI 576

Score = 1012 (151.8 bits), Expect = 4.9e-238, Sum P(2) 4.9e-238 Identities = 194/211 (91%), Positives = 200/211 (94%)

Query: 1 MAPEIHMTGPMCLIENTNGELVANPEALKILSAITQPVVVVAIVGLYRTGKSYLMNKLAG 60

MA EIHMTGPMCLIENTNG L+ANPEALKILSAITQP+VVVAIVGLYRTGKSYLMNKLAG Sbjct: 1 MASEIHMTGPMCLIENTNGRLMANPEALKILSAITQPMVVVAIVGLYRTGKSYLMNKLAG 60

Query: 61 KNKGFSLGSTVKSHTKGIWMWCVPHPKKPEHTLVLLDTEGLGDVKKGDNQNDSWIFTLAV 120

K KGFSLGSTV+SHTKGIWMWCVPHPKKP H LVLLDTEGLGDV+KGDNQNDSWIF LAV Sbjct: 61 KKKGFSLGSTVQSHTKGIWMWCVPHPKKPGHILVLLDTEGLGDVEKGDNQNDSWIFALAV 120

Query: 121 LLSSTLVYNSMGTINQQAMDQLYYVTELTHRIRSKSSPDENENE--DSADFVSFFPDFVW 178

LLSST VYNS+GTINQQAMDQLYYVTELTHRIRSKSSPDENENE DSADFVSFFPDFVW Sbjct: 121 LLSSTFVYNSIGTINQQAMDQLYYVTELTHRIRSKSSPDENENEVEDSADFVSFFPDFVW 180

Query: 179 TLRDFSLDLEADGQPLTPDEYLEYSLKLTQG 209

TLRDFSLDLEADGQPLTPDEYL YΞLKL +G Sbjct: 181 TLRDFSLDLEADGQPLTPDEYLTYSLKLKKG 211

Pedant information for DKFZphfbr2_78c24, frame 3

Report for DKFZphfbr2_78c24.3

[LENGTH] 563

[MW] 64127.72

[pi] 5.45

[HOMOL] PIR:A41268 guanine nucleotide-bmd ng protein 1 - human 0.0

[SUPFAM] guanine nucleotide-bindmg protein 1 0.0

[PROSITE] ATP_GTP_A 1

[PROSITE] RGD 1

[KW] TRANSMEMBRANE 1

[KW] LOW_COMPLEXITY 6.75 %

[KW] COILED COIL 10.48 %

SEQ MAPEIHMTGPMCLIENTNGELVANPEALKILSAITQPVVVVAIVGLYRTGKSYLMNKLAG

SEG

PRD cccccccccceeeeeccccchhhhhhhhhhhhhhhcceeeeeeeecccccchhhhhhhhh

COILS

MEM MMMMMMMMMMMMMMMMM

SEQ KNKGFSLGSTVKSHTKGIWMWCVPHPKKPEHTLVLLDTEGLGDVKKGDNQNDSWIFTLAV

SEG

PRD cccccccccccccccceeeeeecccccccceeeeeeeccccccccccccccchhhhhhhh

COILS

MEM

SEQ LLSSTLVYNSMGTINQQAMDQLYYVTELTHRIRSKSSPDENENEDSADFVSFFPDFVWTL SEG PRD hhhhheeeccccchhhhhhhhhhhhhhhhhhhhhcccccccccccccceeeeccceeeeh COILS

MEM

SEQ RDFSLDLEADGQPLTPDEYLEYSLKLTQGNRKLAQLEKLQDEELDPEFVQQVADFCSYIF SEG PRD hhhhhhhhccccccccchhhhhhhhhhccchhhhhhhhhhhhhcccchhhhhhhhhhhhc COILS MEM

SEQ SNΞKTKTLSGGIKVNGPCLESLVLTYINAISRGDLPCMENAVLALAQIENSAAVQKAIAH

SEG

PRD cccceeeccccccccccchhhhhhhhhhhcccccccchhhhhhhhhhhhhhhhhhhhhhh

COILS

MEM

SEQ YDQQMGQKVQLPAETLQELLDLHRVSEREATEVYMKNSFKDVDHLFQKKLAAQLDKKRDD

SEG

PRD hhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhh

COILS

MEM

SEQ FCKQNQEASSDRCSALLQVIFSPLEEEVKAGIYSKPGGYCLFIQKLQDLEKKYYEEPRKG

SEG

PRD hhhhhhchhhhhhhhhhhhhhhhhhhhhhcccccccccceeehhhhhhhhhhhhhccccc

COILS

MEM

SEQ IQAEEILQTYLKSKESVTDAILQTDQILTEKEKEIEVECVKAESAQASAKMVEEMQIKYQ SEG xxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS

MEM

SEQ QMMEEKEKSYQEHVKQLTEKMERERAQLLEEQEKTLTSKLQEQARVLKERCQGESTQLQN

SEG xxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhh

COILS cccccccccccccccccccccccccccccc cccccccccccccccccccccc

MEM

SEQ EIQKLQKTLKKKTKRYMSHKLKI

SEG .. xxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhccc

COILS CCCCCCC

MEM

Prosite for DKFZphfbr2_78c24.3

PS00016 272->275 RGD PDOC00016 PS00017 45->53 ATP GTP A PDOC00017

(No Pfam data available for DKFZphfbr2_78c24.3)

DKFZphfbr2_78dl3

group: brain derived

DKFZphfbr2_78dl3 encodes a novel 259 amino acid protein with similarity to C. elegans putative protein from cosmid K08B12.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . similarity to C. elegans K08B12.3

Sequenced by MediGenomix

Locus: /map="338.4 cR from top of Chrl8 linkage group"

Insert length: 2195 bp

Poly A stretch at pos. 2175, polyadenylation signal at pos. 2156

1 CGTCCGTCGG GCAGCAGCGG GGCTGTCTAT CCCGGCTGAG GACCCGCGGC

51 CAGTGCGGGT GGCTGGCTTT GCCATTAGCG GGGGCCTTTC CTGAGGACGG

101 CGTACGGAGT GTGGGGAATG AAGGATGGCA GCATGCCGTG CATTAAAAGC

151 TGTTTTGGTA GATCTCAGTG GCACACTTCA CATTGAAGAT GCAGCTGTGC

201 CAGGCGCACA GGAAGCTCTT AAAAGGTTAC GTGGTGCTTC TGTAATCATT

251 AGGTTTGTGA CCAATACAAC CAAAGAGAGC AAGCAAGACC TGTTAGAAAG

301 GTTGAGAAAA TTGGAATTTG ATATCTCTGA AGATGAAATA TTCACATCTC

351 TGACTGCAGC CAGAAGTTTA CTAGAGCGGA AACAAGTCAG ACCCATGCTG

401 CTAGTTGATG ATCGGGCACT ACCTGATTTC AAAGGAATAC AAACAAGTGA

451 TCCTAATGCT GTGGTCATGG GATTGGCACC AGAACATTTT CATTATCAAA

501 TTCTGAATCA AGCATTCCGG TTACTCCTGG ATGGAGCACC TCTGATAGCA

551 ATCCACAAAG CCAGGTATTA CAAGAGGAAA GATGGCTTAG CCCTGGGGCC

601 TGGACCATTT GTGACTGCTT TAGAGTATGC CACAGATACC AAAGCCACAG

651 TCGTGGGGAA ACCAGAGAAG ACGTTCTTTT TGGAAGCATT GCGGGGCACT

701 GGCTGTGAAC CTGAGGAGGC TGTCATGATA GGAGATGATT GCAGGGATGA

751 TGTTGGTGGG GCTCAAGATG TCGGCATGCT GGGCATCTTA GTAAAGACTG

801 GGAAATATCG AGCATCAGAT GAAGAAAAAA TTAATCCACC TCCTTACTTA

851 ACTTGTGAGA GTTTCCCTCA TGCTGTGGAC CACATTCTGC AGCACCTATT

901 GTGAAGCAAT GTGTGCATCT GAAGCAACTT GAAATGCAGC TTCTTATTGT

951 CTGGAATGAA TCCCTTACCA ACTCAGTGCC AGCATCGGTA GACACCAGTC

1001 AGTGCTGATC GCTTTTTAAC CCTCTTTTGT TGTGCATTAA TTAGAAAGAA

1051 AGGTATTGAA TTGCGGCTAG CCAGTAAGCC TTGCTAATCT CTTTTATTTT

1101 GTAACTGAAG ATGAGACCCA AAGAAAGGGA AAGCTGAGAT TTTGTGCCAT

1151 TCCTTTTAAA ATATTCATCA GGTTAGGTGG GGCTGTGGGG GAAAAGCTAC

1201 TACAGGGAAG AGTGTTCTCT GCTGTCTCTT CACTGGAAAA CAGGGAGGGG

1251 GGATTTCAGA CTGTGAAGAA AGTTGAATGG TGGTTTTTAA ATTATAAAGT

1301 AATGTATTAA AAGGTGCATT AGGCTGTAGT TCTAATATTG AGTTCAACTG

1351 TGAAATCCAT CAGATGTGCC AAATGGAGAA GACAGAAAGC AACAAAGTGA

1401 ATTGTTCTTT AGCCCAAGTG GTACAGTGAA TTTGCTTTAA CAGATGTTGA

1451 AAACTAAATT TTCTACTGTA TTCCCAGCAC GGGTGACTTC TTTTTCTCTT

1501 CATTAGCCAG AGATGACTAA TTTAAATTTA GAACCAGATT TTAATTTAAA

1551 TTAATATTTC CATTAATAAC CTACTCATTG CAGATACCTA TTATACTGTG

1601 TAACAGTTGT TTTGGAAATT TTATGTAAAA TTAAAACTAT CAGTATTTTA

1651 CAGATGTTTT AATTAGACAT TGTTATTAAC AGGAACAGTG CAGAAACTAG

1701 AATCAAGCCT TATAATATCT TATAGACCAT GCATTTTTGA AGTTAGTGTC

1751 CACTAGGGTC CTATTAACTG TACATTTGCA AGATTTCATT ATTTTTGCCT

1801 CTGACACTAT GGGAAAAATT TTTTAGAAGC TATTGGGACA GATTCAAGCT

1851 TTTATGCACT TGGTTACTAC AGCTGTAAAA TGAAATCTCG TCTTGTAGCA

1901 TGGATTATTC TTCTCATGTT AAACCCACCA AAATAAAGGG GACTAAATAG

1951 GTAATGATTT TCCTAGTGCA TTTGCATACT GTGATAATCC TGGGCCTTGC

2001 AATAGTTCTA CAGGGCTCTT GGGCATTGAA TTATTAGGAT GTAATTGTAC

2051 ATCATTGTAG TGTTCACCTT ATTGAAGCTC ACTCTGATGT TAATGAGCTT

2101 CGGGTTTTGA TGCTTGTTTA GAGATCAGCA GTCTTGGATG GGAGGGAACA

2151 AAGCTAAATA AATGTTAGTT TGGTGAAAAA AAAAAAAAAA AAAAA

BLAST Results

Entry HS599355 from database EMBL: human STS WI-13484. Score = 1262, P = 3.6e-52, identities = 274/289

Medline entries No Medline entry

Peptide information for frame 2

ORF from 125 bp to 901 bp; peptide length: 259 Category: similarity to unknown protein Classification: no clue

1 MAACRALKAV LVDLSGTLHI EDAAVPGAQE ALKRLRGASV IIRFVTNTTK 51 ESKQDLLERL RKLEFDISED EIFTSLTAAR SLLERKQVRP MLLVDDRALP 101 DFKGIQTSDP NA VMGLAPE HFHYQILNQA FRLLLDGAPL IAIHKARYYK 151 RKDGLALGPG PFVTALEYAT DTKATVVGKP EKTFFLEALR GTGCEPEEAV 201 MIGDDCRDDV GGAQDVGMLG ILVKTGKYRA SDEEKINPPP YLTCESFPHA 251 VDHILQHLL

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_78dl3, frame 2

TREMBL :CEUK08B12_1 gene: "K08B12.3"; Caenorhabditis elegans cosmid K08B12., N = 1, Score = 609, P = 2.2e-59

TREMBL:CEC13C4_5 gene: "C13C4.4"; Caenorhabditis elegans cosmid C13C4, N = 1, Score = 408, P = 4.4e-38

>TREMBL:CEUK08B12_1 gene: "K08B12.3"; Caenorhabditis elegans cosmid K08B12.

Length = 257

HSPs:

Score = 609 (91.4 bits), Expect = 2.2e-59, P = 2.2e-59 Identities = 132/251 (52%), Positives = 172/251 (68%)

Query: 7 LKAVLVDLSGTLHIEDAAVPGAQEALKRLRGASVIIRFVTNTTKESKQDLLERLRKLEFD 66

+ +VL+DLΞGT+HIE+ A+PGAQ AL+ LR + + +FVTNTTKESK+ L +RL F Sbjct: 4 ISSVLIDLSGTIHIEEFAIPGAQTALELLRQHAKV-KFVTNTTKESKRLLHQRLINCGFK 62

Query: 67 IΞEDEIFTSLTAARSLLERKQVRPMLLVDDRALPDFKGIQTSDPNAVVMGLAPEHFHYQI 126

+ ++EIFTSLTAAR L+ + Q RP +VDDRA+ DF+GI T DPNAVV+GLAPE F+ Sbjct: 63 VEKEEIFTSLTAARDLIVKNQYRPFFIVDDRAMEDFEGISTDDPNAVVIGLAPEKFNDTT 122

Query: 127 LNQAFRLLLDG-APLIAIHKARYYKRKDGLALGPGPFVTALEYATDTKATVVGKPEKTFF 185

L AFRL+ + A LIAI+K RY++ GL LGPG +V LEY+ +AT+VGKP K FF Sbjct: 123 LTHAFRLIKEKKASLIAINKGRYHQTNAGLCLGPGTYVAGLEYSAGVEATIVGKPNKLFF 182

Query: 186 LEALRGTG--CEPEEAVMIGDDCRDDVGGAQDVGMLGILVKTGKYRASDEEKINPPPYLT 243

AL+ + AVMIGDD DD GA +GM ILVKTGK+R DE K+ Sbjct: 183 ESALQSLNENVDFSSAVMIGDDVNDDALGAIKIGMRAILVKTGKFRDGDELKVKN V 238

Query: 244 CESFPHAVDHILQH 257

SF AV+ I+++ Sbjct: 239 ANSFVDAVNMIIEN 252

Pedant information for DKFZphfbr2_78dl3, frame 2

Report for DKFZphfbr2_78dl3.2

[LENGTH] 259

[MW] 28536.04

[pi] 5.84

[HOMOL] TREMBL :CEUK08B12_1 gene: "K08B12.3"; Caenorhabditis elegans cosmid K08B12. 3e-

62

[FUNCAT] r general function prediction [M. jannaschn, MJ1437] 3e-05

[SUPFAM] nagD protein 4e-18

[KW] Alpha_Beta SEQ MAACRALKAVLVDLSGTLHIEDAAVPGAQEALKRLRGASVIIRFVTNTTKESKQDLLERL

PRD ccccccceeeeeecccceeeecccccchhhhhhhhhhccceeeeeeccccchhhhhhhhh

SEQ RKLEFDISEDEIFTSLTAARSLLERKQVRPMLLVDDRALPDFKGIQTSDPNAVVMGLAPE

PRD hhhccccccceeeehhhhhhhhhhhhccceeeeeechhhhhhccccccccceeeeecccc

SEQ HFHYQILNQAFRLLLDGAPLIAIHKARYYKRKDGLALGPGPFVTALEYATDTKATVVGKP

PRD chhhhhhhhhhhhhhccceeeeeccccccccccccccccccchhhhhhhhccceeeeccc

SEQ EKTFFLEALRGTGCEPEEAVMIGDDCRDDVGGAQDVGMLGILVKTGKYRASDEEKINPPP

PRD cchhhhhhhhhhccccceeeeecccchhhhhhhhhccceeeeeeeccccccccccccccc

SEQ YLTCESFPHAVDHILQHLL

PRD cccccchhhhhhhhhhccc

(No Prosite data available for DKFZphfbr2_78dl3.2) (No Pfam data available for DKFZphfbr2_78dl3.2)

DKFZphfbr2_78k24

group: metabolism

DKFZphfbr2_78k24 encodes a novel 372 ammo acid protein with similarity to Mus musculus ubiquitin specific protease UBP43.

The novel protein contains a Prosite ubiquitin carboxyl-terminal hydrolases family 2 signature 2. Ubiquitin carboxyl-terminal hydrolases (EC 3.1.2.15) (UCH) (deubiquitinat g enzymes) are thiol proteases that recognize and hydrolyze the peptide bond at the C-terminal glycine of ubiquitin. These enzymes are involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins.

The new protein can find application in modulation of protein stability/degradation in cells.

Ubiquitin carboxyl-terminal hydrolases family 2 signature 2. strong similarity to mouse ubiquitin specific protease UBP43

Sequenced by MediGenomix

Locus: unknown

Insert length: 1874 bp

Poly A stretch at pos. 1852, polyadenylation signal at pos. 1836

1 AGTCCCGACG TGGAACTCAG CAGCGGAGGC TGGACGCTTG CATGGCGCTT

51 GAGAGATTCC ATCGTGCCTG GCTCACATAA GCGCTTCCTG GAAGTGAAGT

101 CGTGCTGTCC TGAACGCGGG CCAGGCAGCT GCGGCCTGGG GGTTTTGGAG

151 TGATCACGAA TGAGCAAGGC GTTTGGGCTC CTGAGGCAAA TCTGTCAGTC

201 CATCCTGGCT GAGTCCTCGC AGTCCCCGGC AGATCTTGAA GAAAAGAAGG

251 AAGAAGACAG CAACATGAAG AGAGAGCAGC CCAGAGAGCG TCCCAGGGCC

301 TGGGACTACC CTCATGGCCT GGTTGGTTTA CACAACATTG GACAGACCTG

351 CTGCCTTAAC TCCTTGATTC AGGTGTTCGT AATGAATGTG GACTTCACCA

401 GGATATTGAA GAGGATCACG GTGCCCAGGG GAGCTGACGA GCAGAGGAGA

451 AGCGTCCCTT TCCAGATGCT TCTGCTGCTG GAGAAGATGC AGGACAGCCG

501 GCAGAAAGCA GTGCGGCCCC TGGAGCTGGC CTACTGCCTG CAGAAGTGCA

551 ACGTGCCCTT GTTTGTCCAA CATGATGCTG CCCAACTGTA CCTCAAACTC

601 TGGAACCTGA TTAAGGACCA GATCACTGAT GTGCACTTGG TGGAGAGACT

651 GCAGGCCCTG TATACGATCC GGGTGAAGGA CTCCTTGATT TGCGTTGACT

701 GTGCCATGGA GAGTAGCAGA AACAGCAGCA TGCTCACCCT CCCACTTTCT

751 CTTTTTGATG TGGACTCAAA GCCCCTGAAG ACACTGGAGG ACGCCCTGCA

801 CTGCTTCTTC CAGCCCAGGG AGTTATCAAG CAAAAGCAAG TGCTTCTGTG

851 AGAACTGTGG GAAGAAGACC CGTGGGAAAC AGGTCTTGAA GCTGACCCAT

901 TTGCCCCAGA CCCTGACAAT CCACCTCATG CGATTCTCCA TCAGGAATTC

951 ACAGACGAGA AAGATCTGCC ACTCCCTGTA CTTCCCCCAG AGCTTGGATT

1001 TCAGCCAGAT CCTTCCAATG AAGCGAGAGT CTTGTGATGC TGAGGAGCAG

1051 TCTGGAGGGC AGTATGAGCT TTTTGCTGTG ATTGCGCACG TGGGAATGGC

1101 AGACTCCGGT CATTACTGTG TCTACATCCG GAATGCTGTG GATGGAAAAT

1151 GGTTCTGCTT CAATGACTCC AATATTTGCT TGGTGTCCTG GGAAGACATC

1201 CAGTGTACCT ACGGAAATCC TAACTACCAC TGGCAGGAAA CTGCATATCT

1251 TCTGGTTTAC ATGAAGATGG AGTGCTAATG GAAATGCCCA AAACCTTCAG

1301 AGATTGACAC GCTGTCATTT TCCATTTCCG TTCCTGGATC TACGGAGTCT

1351 TCTAAGAGAT TTTGCAATGA GGAGAAGCAT TGTTTTCAAA CTATATAACT

1401 GAGCCTTATT TATAATTAGG GATATTATCA AAATATGTAA CCATGAGGCC

1451 CCTCAGGTCC TGATCAGTCA GAATGGATGC TTTCACCAGC AGACCCGGCC

1501 ATGTGGCTGC TCGGTCCTGG GTGCTCGCTG CTGTGCAAGA CATTAGCCCT

1551 TTAGTTATGA GCCTGTGGGA ACTTCAGGGG TTCCCAGTGG GGAGAGCAGT

1601 GGCAGTGGGA GGCATCTGGG GGCCAAAGGT CAGTGGCAGG GGGTATTTCA

1651 GTATTATACA ACTGCTGTGA CCAGACTTGT ATACTGGCTG AATATCAGTG

1701 CTGTTTGTAA TTTTTCACTT TGAGAACCAA CATTAATTCC ATATGAATCA

1751 AGTGTTTTGT AACTGCTATT CATTTATTCA GCAAATATTT ATTGATCATC

1801 TCTTCTCCAT AAGATAGTGT GATAAACACA GTCATGAATA AAGTTATTTT

1851 CCACAAAAAA AAAAAAAAAA AAAA

BLAST Results

Entry AC005500 from database EMBL: , complete sequence.

Score = 859, P = 5.7e-143, identities = 175/179 8 exons matching Bp 317-1230 Medlme entries

99182491:

A novel ubiquitm-specific protease, UBP43, cloned from leukemia fusion protein AMLl-ETO-expressing mice, functions in hematopoietic cell differentiation.

Peptide information for frame 1

ORF from 160 bp to 1275 bp; peptide length: 372 Category: strong similarity to known protein Classification: Protein management Prosite motifs: UCH 2 2 (302-320)

1 MSKAFGLLRQ ICQSILAESS QSPADLEEKK EEDSNMKREQ PRERPRAWDY

51 PHGLVGLHNI GQTCCLNSLI QVFVMNVDFT RILKRITVPR GADEQRRSVP

101 FQMLLLLEKM QDSRQKAVRP LELAYCLQKC NVPLFVQHDA AQLYLKLWNL

151 IKDQITDVHL VERLQALYTI RVKDSLICVD CAMESSRNSS MLTLPLSLFD

201 VDSKPLKTLE DALHCFFQPR ELSSKSKCFC ENCGKKTRGK QVLKLTHLPQ

251 TLTIHLMRFS IRNSQTRKIC HSLYFPQSLD FSQILPMKRE SCDAEEQSGG

301 QYELFAVIAH VGMADSGHYC VYIRNAVDGK WFCFNDSNIC LVSWEDIQCT

351 YGNPNYHWQE TAYLLVYMKM EC

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_78k24, frame 1

TREMBLNEW:AF069502_1 product: "ubiquitin specific protease UBP43"; Mus musculus ubiquitin specific protease UBP43 mRNA, complete eds., N = 1, Score = 1367, P = le-139

SWISSPROT :UBPE_DROME UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 64E (EC 3.1.2.15) (UBIQUITIN THIOLESTERASE 64E) (UBIQUITIN-SPECIFIC PROCESSING PROTEASE 64E) (DEUBIQUITINATING ENZYME 64E) . , N = 2, Score = 248, P = 5.3e-33

>TREMBLNEW:AF069502_1 product: "ubiquitin specific protease UBP43"; Mus musculus ubiquitin specific protease UBP43 mRNA, complete eds. Length = 368

HSPs:

Score = 1367 (205.1 bits), Expect = 1.0e-139, P = 1.0e-139 Identities = 262/369 (71%), Positives = 295/369 (79%)

Query: 1 MSKAFGLLRQICQSILAESSQSPADLEEKKEEDSNMKREQPRERPRAWDYPHGLVGLHNI 60

M K FGLLR+ CQS++AE Q A LEE E KR R+ AWD PHGLVGLHNI Sbjct: 1 MGKGFGLLRKPCQSVVAEPQQYSA-LEE—ERTMKRKRVLSRDLCSAWDSPHGLVGLHNI 57

Query: 61 GQTCCLNSLIQVFVMNVDFTRILKRITVPRGADEQRRSVPFQMLLLLEKMQDSRQKAVRP 120

GQTCCLNSL+QVF+MN+DF ILKRITVPR A+E++RSVPFQ+LLLLEKMQDSRQKA+ P Sbjct: 58 GQTCCLNSLLQVFMMNMDFRMILKRITVPRSAEERKRSVPFQLLLLLEKMQDSRQKALLP 117

Query: 121 LELAYCLQKCNVPLFVQHDAAQLYLKLWNLIKDQITDVHLVERLQALYTIRVKDSLICVD 180

EL CLQK NVPLFVQHDAAQLYL +WNL KDQITD L ERLQ L+TI ++SLICV Sbjct: 118 TELVQCLQKYNVPLFVQHDAAQLYLTIWNLTKDQITDTDLTERLQGLFTIWTQESLICVG 177

Query: 181 CAMESSRNSSMLTLPLSLFDVDSKPLKTLEDALHCFFQPRELSSKSKCFCENCGKKTRGK 240

C ESSR S +LTL L LFD D+KPLKTLEDAL CF QP+EL+S C CE CG+KT K Sbjct: 178 CTAESSRRSKLLTLSLPLFDKDAKPLKTLEDALRCFVQPKELASSDMC-CETCGEKTPWK 236

Query: 241 QVLKLTHLPQTLTIHLMRFSIRNSQTRKICHSLYFPQSLDFSQILPMKRESCDAEEQSGG 300

QVLKLTHLPQTLTIHLMRFS RNS+T KICHS+ FPQSLDFSQ+LP + + D +EQS Sbjct: 237 QVLKLTHLPQTLTIHLMRFSARNSRTEKICHSVNFPQSLDFSQVLPTEEDLGDTKEQSEI 296

Query: 301 QYELFAVIAHVGMADSGHYCVYIRNAVDGKWFCFNDSNICLVSWEDIQCTYGNPNYHWQE 360

YELFAVIAHVGMAD GHYC YIRN VDGKWFCFNDS++C V+W+D+QCTYGN Y W+E Sbjct: 297 HYELFAVIAHVGMADFGHYCAYIRNPVDGKWFCFNDSHVCWVTWKDVQCTYGNHRYRWRE 356

Query: 361 TAYLLVYMK 369 TAYLLVY K Sbjct: 357 TAYLLVYTK 365

Pedant information for DKFZphfbr2_78k24, frame 1

Report for DKFZphfbr2_78k24.1

[LENGTH] 372

[MW] 43011.12

[pi] 8.05

[HOMOL] TREMBLNEW:AF069502_1 product: "ubiquitin specific protease UBP43 Mus musculus ubiquitin specific protease UBP43 mRNA, complete eds. le-151

[FUNCAT] 06.13 proteolysis [S. cerevisiae, YMR304w] 3e-19

[FUNCAT] 06.13.01 cytoplasmic degradation [S. cerevisiae, YJL197w] 3e-16

[FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YMR223w] le-15

[FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YNL186w] 6e-12

[FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YDR069c] 9e-ll

[FUNCAT] 10.03.99 other osmosensing activities [S. cerevisiae, YDR069c] 9e-ll

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YDR069c] 9e-ll

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDR069c] 9e-ll

[FUNCAT] 09.25 vacuolar and lysosomal biogenesis [S. cerevisiae, YDR069c] 9e-ll

[BLOCKS] BL00582A Ribosomal protein L33 proteins

[BLOCKS] BL00972E

[BLOCKS] BL00972D

[BLOCKS] BL00972A

[EC] 2.4.2.29 Queuine tRNA-ribosyltransferase le-06

[PIRKW] pentosyltransferase le-06

[PIRKW] glycosyltransferase le-06

[PIRKW] tRNA modification le-06

[PIRKW] alternative splicing 7e-ll

[PIRKW] hydrolase 7e-06

[SUPFAM] deubiquinatmg enzyme SSV7 2e-09

[PROSITE] UCH_2_21

[PFAM] Ubiquitin carboxyl-terminal hydrolases family 2

[PFAM] Ubiquitin carboxyl-terminal hydrolases family 2

[KW] Alpha_Beta

SEQ MSKAFGLLRQICQSILAESSQSPADLEEKKEEDSNMKREQPRERPRAWDYPHGLVGLHNI PRD cccceeechhhhhhhhcccccccchhhhhhhhcccccccccccccccccccccccccccc

SEQ GQTCCLNSLIQVFVMNVDFTRILKRITVPRGADEQRRSVPFQMLLLLEKMQDSRQKAVRP PRD cceeehhhhhhhhhcccchhhhhhhcccccccccchhhhhhhhhhhhhhhhhhhhccccc

SEQ LELAYCLQKCNVPLFVQHDAAQLYLKLWNLIKDQITDVHLVERLQALYTIRVKDSLICVD PRD hhhhhccccccccchhhhhhhhhhhhhhhhhhhhhhcchhhhhhhhhhhhhhhhheeeee

SEQ CAMESSRNSSMLTLPLSLFDVDSKPLKTLEDALHCFFQPRELSSKSKCFCENCGKKTRGK PRD ccccccccccccccccccccccccchhhhhhhhhhhhhhcccccccceeecccccccccc

SEQ QVLKLTHLPQTLTIHLMRFSIRNSQTRKICHSLYFPQSLDFSQILPMKRESCDAEEQSGG PRD cceeeecccchhhhhhhhhhhccchhhhhccccccccccccccccccccccccccccccc

SEQ QYELFAVIAHVGMADSGHYCVYIRNAVDGKWFCFNDSNICLVSWEDIQCTYGNPNYHWQE PRD eeeeeeeeeeeccccccceeeeeecccccceeeeccceeeeeecccccccccccccchhh

SEQ TAYLLVYMKMEC PRD hhhhhhhhhccc

Prosite for DKFZphfbr2_78k24.1

PS00973 302->320 UCH 2 2 PDOC00750

Pfam for DKFZphfbr2_78k24.1

HMM_NAME Ubiquitin carboxyl-terminal hydrolases family 2

HMM *GIqNlGNTCYMNSIIQCL* G+ N+G TC +NS+IQ+

Query 56 GLHNIGQTCCLNSLIQVF 73 HMM_NAME Ubiquitm carboxyl-terminal hydrolases family 2

HMM *YdLYgVICHYGntldyGHYWaYVKNenhHRWkWYYFDDEtV*

Y+L++VI H G D+GHY +Y++N ++KW++F+D+++ Query 302 YELFAVIAHVG-MADSGHYCVYIRNAV--DGKWFCFNDSNI 339

DKFZphfbr2_78n23

group: brain derived

DKFZphfbr2_78n23 encodes a novel 329 amino acid protein with similarity to A. thaliana F26P21.80 protein.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes .

similarity to A. thaliana F26P21.80

Sequenced by MediGenomix

Locus: /map="89.1 cR from top of Chrl9 linkage group"

Insert length: 1447 bp

Poly A stretch at pos. 1374, polyadenylation signal at pos. 1353

1 TACAACTTCC GGCTGTAAAG ATGGCGGCTT CCTAGTGAGT CGGCGGCTGA

51 CTTAGAAGGA GGTTCAGGCT ACGGTGAGCC GAAGCCACAC AGGAGCCATG

101 GAAGTGGCAG AGCCCAGCAG CCCCACTGAA GAGGAGGAGG AGGAAGAGGA

151 GCACTCGGCA GAGCCTCGGC CCCGCACTCG CTCCAATCCT GAAGGGGCTG

201 AGGACCGGGC AGTAGGGGCA CAGGCCAGCG TGGGCAGCCG CAGCGAGGGT

251 GAGGGTGAGG CCGCCAGTGC TGATGATGGG AGCCTCAACA CTTCAGGAGC

301 CGGCCCTAAG TCCTGGCAGG TGCCCCCGCC AGCCCCTGAG GTCCAAATTC

351 GGACACCAAG GGTCAACTGT CCAGAGAAAG TGATTATCTG CCTGGACCTG

401 TCAGAGGAAA TGTCACTGCC AAAGCTGGAG TCGTTCAACG GCTCCAAAAC

451 CAACGCCCTC AATGTCTCTC AGAAGATGAT TGAGATGTTC GTGCGGACAA

501 AACACAAGAT CGACAAAAGC CACGAGTTTG CACTGGTGGT GGTGAACGAT

551 GACACGGCCT GGCTGTCTGG CCTGACCTCC GACCCCCGCG AGCTCTGTAG

601 CTGCCTCTAT GATCTGGAGA CGGCCTCCTG TTCCACCTTC AATCTGGAAG

651 GACTTTTCAG CCTCATCCAG CAGAAAACTG AGCTTCCGGT CACAGAGAAC

701 GTGCAGACGA TTCCCCCGCC ATATGTGGTC CGCACCATCC TTGTCTACAG

751 CCGTCCACCT TGCCAGCCCC AGTTCTCCTT GACGGAGCCC ATGAAGAAAA

801 TGTTCCAGTG CCCATATTTC TTCTTTGACG TTGTTTACAT CCACAATGGC

851 ACTGAGGAGA AGGAGGAGGA GATGAGTTGG AAGGATATGT TTGCCTTCAT

901 GGGCAGCCTG GATACCAAGG GTACCAGCTA CAAGTATGAG GTGGCACTGG

951 CTGGGCCAGC CCTGGAGTTG CACAACTGCA TGGCGAAACT GTTGGCCCAC

1001 CCCCTGCAGC GGCCTTGCCA GAGCCATGCT TCCTACAGCC TGCTGGAGGA

1051 GGAGGATGAA GCCATTGAGG TTGAGGCCAC TGTCTGAACC ATCCCTGTAC

1101 ATCTGCACCT TCTTGTGCAA GGAAGTCCTT GGCCTAAAGC CTTGGTTCTC

1151 AAACTGGGTT CCTTGGGACC TCCGGGGTGG GGGGGTTCCA GGAGGCACGT

1201 AGGGTACCTT GCAGGGTCCT AGGAGGGAAA CCCAGGATTC CAGGAGGGAT

1251 CCCAGGAACT GTGGGCACCC ATTTTCTGTG TCTCCCAGCC CATTTCCACT

1301 CCTAGTTTGT CATGGATAAT TTTTGTTCTT CCCTGTGTGA TTTTTGCCAT

1351 CAAAATAAAA ATTTGAGACT CGTTAAAAAA AAAAAAAAAA AAAAAAAAAA

1401 AAAAAAAAAA AAAAAAAAAA AAAAAAGAAA AAAAAAAAAA AAAAAAA

BLAST Results

Entry HS806352 from database EMBL: human STS EST192543. Score = 1285, P = 2.5e-51, identities 263/266

Medl e entries

No Medline entry

Peptide information for frame 2

ORF from 98 bp to 1084 bp; peptide length: 329 Category: similarity to unknown protein Classification: no clue

1 MEVAEPSSPT EEEEEEEEHS AEPRPRTRSN PEGAEDRAVG AQASVGSRSE 51 GEGEAASADD GSLNTSGAGP KSWQVPPPAP EVQIRTPRVN CPEKVIICLD

101 LSEEMSLPKL ESFNGSKTNA LNVSQKMIEM FVRTKHKIDK SHEFALVVVN

151 DDTAWLSGLT SDPRELCSCL YDLETASCST FNLEGLFSLI QQKTELPVTE

201 NVQTIPPPYV VRTILVYSRP PCQPQFSLTE PMKKMFQCPY FFFDVVYIHN

251 GTEEKEEEMS WKDMFAFMGS LDTKGTSYKY EVALAGPALE LHNCMAKLLA

301 HPLQRPCQSH ASYSLLEEED EAIEVEATV

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_78n23, frame 2

PIR:T05304 hypothetical protein F26P21.80 - Arabidopsis thaliana, N = 1, Score = 142, P = 1.5e-07

>PIR:T05304 hypothetical protein F26P21.80 - Arabidopsis thaliana Length = 264

HSPs:

Score = 142 (21.3 bits), Expect = 1.5e-07, P = 1.5e-07 Identities = 56/216 (25%), Positives = 97/216 (44%)

Query: 93 EKVIICLDL-SEEMSLPKLESFNGSKTNALNVSQKMIEMFVRTKHKIDKSHEFALVVVND 151

E ++IC+D+ +E M K NG + ++ I +F+ K 1+ H FA + Sbjct: 26 EDILICIDVDAEΞMVEMKTTGTNGRPLIRMECVKQAIILFIHNKLSINPDHRFAFATLAK 85

Query: 152 DTAWLSG-LTSDPRELCSCLYDLE-TASCSTFNLEGLFSLIQQKTELPVTENVQTIPPPY 209

AWL TSD + L L S S +L LF Q+ ++ +N Sbjct: 86 SAAWLKKEFTSDAESAVASLRGLSGNKΞSSRADLTLLFRAAAQEAKVSRAQN R 138

Query: 210 VVRTILVYSRPPCQPQFΞLTEPMKKMFQCPYFFFDVVYIHNGTEEKEEEMSWKDMF-AFM 268

+ R IL+Y R +P P+ + F DV+Y+H ++ + +D++ + + Sbjct: 139 IFRVILIYCRSSMRPTHEW—PLNQKL FTLDVMYLH DKPSPDNCPQDVYDSLV 189

Query: 269 GSLD--TKGTSYKYEVALAGPALELHNCMAKLLAHPLQRPCQ 308

+++ ++ Y +E G A + M+ LL HP QR Q Sbjct: 190 DAVEHVSEYEGYIFESG-QGLARSVFKPMSMLLTHPQQRCAQ 230

Pedant information for DKFZphfbr2_78n23, frame 2

Report for DKFZphfbr2_78n23.2

[LENGTH] 329

[MW] 36560.10

[pi] 4.60

[HOMOL] PIR:T05304 hypothetical protein F26P21.80 - Arabidopsis thaliana 7e-07

[KW] Alpha_Beta

[KW] LOW_COMPLEXITY 9.73 %

SEQ MEVAEPSSPTEEEEEEEEHSAEPRPRTRSNPEGAEDRAVGAQASVGSRSEGEGEAASADD SEG . xxxxxxxxxxxxxxxxxxxxxx

PRD cccccccccchhhhhhhhhhhccccccccccccchhhhhhhhhhhccccccccccccccc

SEQ GSLNTSGAGPKSWQVPPPAPEVQIRTPRVNCPEKVIICLDLSEEMSLPKLESFNGSKTNA SEG

PRD ccccccccccccccccccccceeeccccccccceeeeeccccccccccccccccccccee

SEQ LNVSQKMIEMFVRTKHKIDKSHEFALVVVNDDTAWLSGLTSDPRELCΞCLYDLETASCST SEG

PRD ehhhhhhhhhhhhhhhccccccceeeeeeccchhhhhcccccchhhhhhhhhcccccccc

SEQ FNLEGLFSLIQQKTELPVTENVQTIPPPYVVRTILVYSRPPCQPQFSLTEPMKKMFQCPY SEG

PRD hhhhhhhhhhhhhhhhhhhhhcccccccccceeeeeeecccccccccccchhhhhheeee

SEQ FFFDVVYIHNGTEEKEEEMSWKDMFAFMGSLDTKGTSYKYEVALAGPALELHNCMAKLLA SEG

PRD eeeeeeeeccccchhhhhhhhhhhhhhhhcccccccceeeeecccccchhhhhhhhhhhh

SEQ HPLQRPCQSHASYSLLEEEDEAIEVEATV

SEG xxxxxxxxxx ...

PRD hcccccccccchhhhhhhhhhhhhhhccc (No Prosite data available for DKFZphfbr2_78n23.2) (No Pfam data available for DKFZphfbr2_78n23.2)

DKFZphfbr2_7a24

group: brain derived

DKFZphfbr2_7a24 encodes a novel 142 ammo acid protein with similarity to the C-terminal part of transforming growth factor-beta activated kinases.

The novel protein shows only similarity to the C-termmus of such kinases; no kinase domain is present .

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . similarity to C-termmus of TGF-beta-activated kinase complete CDNA, complete eds, EST hits

Sequenced by GBF

Locus: unknown

Insert length: 1697 bp

No poly A stretch found, no polyadenylation signal found

1 GGGGAGAGAG GGGTTGTGAA GGGAAGCGGA AGGGAAGGGA AGGGAGGTCC

51 CGTGGGACGC TGGGGTCTGG GGTAGAGCAG GTAGCAGCGT GCTGCCCTGA

101 CAGCTGTCTC CGCTCCTCAG ATTGTCAGTG GCTGCTATGC AGCAGGTGCA

151 GCCTGGTCTC TCACTGAGTC TCTACTCCAC AAAGGCAACG ACTGGCCAAG

201 GCAGTGGCTG GCTCTGGGTT ACACAAGTGC AGACACTCAA CTAAGTGAGC

251 TGGAAGACCC AGGAGAAGGC GGAGGCTCAG GTGCCCACAT GATCAGCACA

301 GCCAGGGTAC CTGCTGACAA GCCTGTACGC ATCGCCTTTA GCCTCAATGA

351 CGCCTCAGAT GATACACCCC CTGAAGACTC CATTCCTTTG GTCTTTCCAG

401 AATTAGACCA GCAGCTACAG CCCCTGCCGC CTTGTCATGA CTCCGAGGAA

451 TCCATGGAGG TGTTCAGACA GCACTGCCAA ATAGCAGAAG AATACCTTGA

501 GGTCAAAAAG GAAATCACCC TGCTTGAGCA AAGGAAGAAG GAGCTCATTG

551 CCAAGTTAGA TCAGGCAGAA GAGGAGAAGG TGGATGCTGC TGAGCTGGTT

601 CGGGAATTCG AGGCTCTGAC GGAGGAGAAT CGGACGTTGA GGTTGGCCCA

651 GTCTCAATGT GTGGAACAAC TGGAGAAACT TCGAATACAG TATCAGAAGA

701 GGCAGGGCTC GTCCTAACTT TAAATTTTTC AGTGTGAGCA TACGAGGCTG

751 ATGACTGCCC TGTGCTGGCC AAAAGATTTT TATTTTAAAT GAATAGTGAG

801 TCAGATCTAT TGCTTCTCTG TATTACCCAC ATGACAACTG TCTATAATGA

851 GTTTACTGCT TGCCAGCTTC TAGCTTGAGA GAAGGGATAT TTTAAATGAG

901 ATCATTAACG TGAAACTATT ACTAGTATAT GTTTTTGGAG ATCAGAATTC

951 TTTTCCAAAG ATATATGTTT TTTTCTTTTT TAGGAAGATA TGATCATGCT

1001 GTACAACAGG GTAGAAAATG GTAAAAATAG ACTATTGACT GACCCAGCTA

1051 AGAATCGCGG GCTGAGCAGA GTTAAACCAT GGGACAAACC CATAACATGT

1101 TCACCATAGT TTCACGTATG TGTATTTTTA AATTTCATGC CTTTAATATT

1151 TCAAATATGC TCAAATTTAA ACTGTCAGAA ACTTCTCTGC ATGTATTTAT

1201 ATTTGCCAGA GTATAAACTT TTATACTCTG ATTTTTATCC TTCAATGATT

1251 GATTATACTA AGAATAAATG GTCACATATC CTAAAAGCTT CTTCATGAAA

1301 TTATTAGCAG AAACCATGTT TGAAACCAAA GCACATTTGC CAATGCTAAC

1351 TGGCTGTTGT AATAATAAAC AGATAAGGCT GCATTTGCTT CATGCCATGT

1401 GACCTCACAG TAAACATCTC TGCCTTTGCC TGTGTGTGTT CTGGGGGAGG

1451 GGGGACATGG AAAAATATTG TTTGGACATT ACTTGGGTGA GTGCCCATGA

1501 AGACATCAGT GAACTTGTAA CTATTGTTTT GTTTTGGATT TAAGGAGATG

1551 TTTTAGATCA GTAACAGCTA ATAGGAATAT GCGAGTAAAT TCAGAATTGA

1601 AACAATTTCT CCTTGTTCTA CCTATCACCA CATTTTCTCA AATTGAACTC

1651 TTTGTTATAT GTCCATTTCT ATTCATGTAA CTTCTTTTTC ATTAAAC

BLAST Results

No BLAST result

Medline entries

98130593:

Role of TAK1 and TAB1 in BMP signaling in early Xenopus development . Peptide information for frame 1

ORF from 289 bp to 714 bp; peptide length: 142 Category: similarity to known protein

1 MISTARVPAD KPVRIAFSLN DASDDTPPED SIPLVFPELD QQLQPLPPCH 51 DSEESMEVFR QHCQIAEEYL EVKKEITLLE QRKKELIAKL DQAEEEKVDA 101 AELVREFEAL TEENRTLRLA QSQCVEQLEK LRIQYQKRQG SS

BLASTP hits

Entry U92030_l from database TREMBL: product: "TAK1"; Xenopus laevis TGF-beta-activated kinase TAK1 mRNA, complete eds.

Score = 343, P = 1.3e-30, identities = 69/143, positives = 104/143

Entry AB009356_1 from database TREMBL: product: "TGF-beta activated kinase la"; Homo sapiens mRNA for

TGF-beta activated kinase la, complete eds.

Score = 339, P = 2.6e-30, identities = 67/143, positives = 104/143

Entry MMPK_1 from database TREMBL: product: "TAK1 (TGF-beta-activated kinase)"; Mouse mRNA for TAK1

(TGF-beta-activated kinase), complete eds.

Score = 339, P = 2.6e-30, identities = 67/143, positives = 104/143

Entry AB009357_1 from database TREMBL: product: "TGF-beta activated kinase lb"; Homo sapiens mRNA for

TGF-beta activated kinase lb, complete eds.

Score = 339, P = 3.2e-30, identities = 67/143, positives = 104/143

Entry AB009358_1 from database TREMBL: product: "TGF-beta activated kinase lc"; Homo sapiens mRNA for

TGF-beta activated kinase lc, complete eds.

Score = 144, P = 3.8e-09, identities = 30/67, positives = 47/67

Alert BLASTP hits for DKFZphfbr2_7a24, frame 1

PIR:JC5955 transforming growth factor-beta activated kinase (EC -.-.-.-) la - Human, N = 1, Score = 339, P = 3e-30

>PIR:JC5955 transforming growth factor-beta activated kinase (EC -.-.-.-) la - Human

Length = 579

HSPs:

Score = 339 (50.9 bits), Expect = 3.0e-30, P = 3.0e-30 Identities = 67/143 (46%), Positives = 104/143 (72%)

Query: 1 MISTARVPADKPVRI-AFSLNDASDDTPPEDSIPLVFPELDQQLQPLPPCHDSEESMEVF 59

MI+T+ ++KP R ++ +D++D ++SIP+ + LD QLQPL PC +S+ESM VF Sbjct: 437 MITTSGPTSEKPTRSHPWTPDDSTDTNGSDNSIPMAYLTLDHQLQPLAPCPNSKESMAVF 496

Query: 60 RQHCQIAEEYLEVKKEITLLEQRKKELIAKLDQAEEEKVDAAELVREFEALTEENRTLRL 119

QHC++A+EY++V+ El LL QRK+EL+A+LDQ E+++ + + LV+E + L +EN++L Sbjct: 497 EQHCKMAQEYMKVQTEIALLLQRKQELVAELDQDEKDQQNTSRLVQEHKKLLDENKSLST 556

Query: 120 AQSQCVEQLEKLRIQYQKRQGSS 142

QC +QLE +R Q QKRQG+Ξ Sbjct: 557 YYQQCKKQLEVIRSQQQKRQGTS 579

Pedant information for DKFZphfbr2_7a24, frame 1

Report for DKFZphfbr2_7a24.1

[LENGTH] 142 [MW] 16377.53

[pi] 4.64

[HOMOL] TREMBL :U92030_1 product: "TAK1"; Xenopus laevis TGF-beta-activated kinase TAK1 mRNA, complete eds. 6e-26 [PROSITE] CK2_PHOSPHO_SITE 3 [PROSITE] PKC_PHOSPHO_SITE 2

[PROSITE] ASN_GLYCOSYLATION 1

[PFAM] TNFR/NGFR cysteme-rich region

[KW] All_Alpha

[KW] LOW_COMPLEXITY 7.04 %

[KW] COILED COIL 33.10 %

SEQ MISTARVPADKPVRIAFSLNDASDDTPPEDSIPLVFPELDQQLQPLPPCHDSEESMEVFR SEG xxxxxxxxxx PRD ccccccccccccccccccccccccccccccccccchhhhhhhhcccccccccchhhhhhh COILS

SEQ QHCQIAEEYLEVKKEITLLEQRKKELIAKLDQAEEEKVDAAELVREFEALTEENRTLRLA SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhh COILS ...CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ QΞQCVEQLEKLRIQYQKRQGSS SEG PRD hhhhhhhhhhhhhhhhhhhccc COILS

Prosite for DKFZphfbr2_7a24.1

PS00001 114->118 ASN GLYCOSYLATION PDOC00001

PS00005 4->7 PKC PHOSPHO SITE PDOC00005

PS00005 116->119 PKC PHOSPHO SITE PDOC00005

PS00006 18->22 CK2 PHOSPHO SITE PDOC00006

PS00006 26->30 CK2 PHOSPHO SITE PDOC00006

PS00006 77->81 CK2 PHOSPHO SITE PDOC00006

Pfam for DKFZphfbr2_7a24.1

HMM_NAME TNFR/NGFR cysteine-rich region

HMM *CpeGtYtDWNHvpqClpCtrCePEMGQYMvqPCTwTQNTVC*

C++++ + + +Q C++ E+ ++++++ T + ++ Query 49 CHDSEESMEVF-RQH—CQIAEE—YLEVKKEITLLEQRKK 84

DKFZphfbr2_7e22

group: brain derived

DKFZphfbr2_7e22.2 encodes a novel 286 ammo acid protein similar to b561 cytochromes

The new protein shows strong similarity to B561 cytochromes, but contains no heme binding site. In addition, a myc-type, helix-loop-helix dimerization domain domain is present. This helix-loop-helix domain mediates protein dimerization and has been found in proteins such as the myc family of cellular oncogenes, proteins involved in myogenesis and vertebrate proteins that bind specific DNA sequences in various lmmunoglobulin chains enhancers.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of bram-specific genes . strong similarity to cytochrome b561 complete cDNA, complete eds, EST hits

Sequenced by GBF

Locus : unknown

Insert length: 4254 bp

Poly A stretch at pos. 4234, polyadenylation signal at pos. 4217

1 GGGGACTACC CAGAGGGCTG CCGCCGCCTC TCCAAGTTCT TGTGGCCCCC 51 GCGGTGCGGA GTATGGGGCG CTGATGGCCA TGGAGGGCTA CCGGCGCTTC

101 CTGGCGCTGC TGGGGTCGGC ACTGCTCGTC GGCTTCCTGT CGGTGATCTT

151 CGCCCTCGTC TGGGTCCTCC ACTACCGAGA GGGGCTTGGC TGGGATGGGA

201 GCGCACTAGA GTTTAACTGG CACCCAGTGC TCATGGTCAC CGGCTTCGTC

251 TTCATCCAGG GCATCGCCAT CATCGTCTAC AGACTGCCGT GGACCTGGAA

301 ATGCAGCAAG CTCCTGATGA AATCCATCCA TGCAGGGTTA AATGCAGTTG

351 CTGCCATTCT TGCAATTATC TCTGTGGTGG CCGTGTTTGA GAACCACAAT

401 GTTAACAATA TAGCCAATAT GTACAGTCTG CACAGCTGGG TTGGACTGAT

451 AGCTGTCATA TGCTATTTGT TACAGCTTCT TTCAGGTTTT TCAGTCTTTC

501 TGCTTCCATG GGCTCCGCTT TCTCTCCGAG CATTTCTCAT GCCCATACAT

551 GTTTATTCTG GAATTGTCAT CTTTGGAACA GTGATTGCAA CAGCACTTAT

601 GGGATTGACA GAGAAACTGA TTTTTTCCCT GAGAGATCCT GCATACAGTA

651 CATTCCCGCC AGAAGGTGTT TTCGTAAATA CGCTTGGCCT TCTGATCCTG

701 GTGTTCGGGG CCCTCATTTT TTGGATAGTC ACCAGACCGC AATGGAAACG

751 TCCTAAGGAG CCAAATTCTA CCATTCTTCA TCCAAATGGA GGCACTGAAC

801 AGGGAGCAAG AGGTTCCATG CCAGCCTACT CTGGCAACAA CATGGACAAA

851 TCAGATTCAG AGTTAAACAA TGAAGTAGCA GCAAGGAAAA GAAACTTAGC

901 TCTGGATGAG GCTGGGCAGA GATCTACCAT GTAAAATGTT GTAGAGATAG

951 AGCCATATAA CGTCACGTTT CAAAACTAGC TCTACAGTTT TGCTTCTCCT 1001 ATTAGCCATA TGATAATTGG GCTATGTAGT ATCAATATTT ACTTTAATCA 1051 CAAAGGATGG TTTCTTGAAA TAATTTGTAT TGATTGAGGC CTATGAACTG 1101 ACCTGAATTG GAAAGGATGT GATTAATATA AATAATAGCA GATATAAATT 1151 GTGGTTATGT TACCTTTATC TTGTTGAGGA CCACAACATT AGCACGGTGC 1201 CTTGTGCAGA ATAGATACTC AATATGTGAA TATGTGTCTA CTAGTAGTTA 1251 ATTGGATAAA CTGGCAGCAT CCCTGGCCTG TTGTCATGCA GTCATTTCCT 1301 GTTAATTCTG GGAGACAATG ATTTCACAAC TAGAGGGAAG CAGTCCTAAA 1351 AGTTTAAAAT CCGATAAGGA ATATCTGGGA CAGGGTTTAG ATCATGACTC 1401 TACACAGATA CCATGATGAG AGTATATTAA AGAAATTTAG GAAAGCACCT 1451 GGTTCCTTTC TCCCCATGCC TGCCTTCTGC TCCCTCCCCA GCTGGTTTGG 1501 GCTCAAATTG TCCCTGGAGA CTAGGGTTTA TGTTAGGGTA TTGATAGATT 1551 AGAGCAGGTG GTTGAAGAGA TCTTCTCTGG TCAGACTTGG AAGAATTTCC 1601 AAAAGTGAAG TTAGCCCCAA GACTTCCCTA GGGTTGATGT ACTTTATGAT 1651 CCAGATGCTA AACTTCTTAG AATGAAAATA TGCTTCAACA CTTAAGTAGC 1701 ATACACTGCC CTACAAACCT CAGAGAGCAC TTTTCCCCAA GTTCTTGTTT 1751 TTATTTTTGA AAGTACTCAC ACAGCACTTA CTATGCTCCA AACACTCCTC 1801 TAAGCACTTT ACACATATTA GCTCATTCAG TCCCCAGACA GACGGGATGA 1851 AGTAGGTATT GTTACTGTTC CCATTTTACA GGTGAGAGAT TTGAAGCCTG 1901 GGGAGGCTAG TAACTCACCC CAAGGTCACA CGGCTCATAC ATGGTGGGAC 1951 TGAGACTCAG ATGCAGGCAG TCTGGCACCT CAGTCTGGAT TCTAACCATT 2001 TCACTAAGCT ATTTTTGTCT TGTACTACTT TGACCCACCC CTGAATAAAC 2051 CTCAATTGCT GGAGTGGGGT GTAGTTATTA AAGGGATGCT TTTTACCTTT 2101 TGCTGTCTGC TGTGGCAGAT TCCCCAGATA ACCAAGGAAA AGGGGCCACC 2151 CATACCTGGA AATAGGCCAT AGGGCCCCTA CTACTGCCAA CAAGCCATGG 2201 CCTACCTTGA CACTTGTTTG ATCTTAAAAT TGTGTCTTGG TAACAAAAGA 2251 TTTGGACAGG CATATCTGTA GCTTTCAAGT TAATTAATTG CAATATTTTT 2301 TTCTTCAGGA TTTTAGCTGC TGAACAACTT TCAGTTTGGA GCTAAAAGAG 2351 ACCTGTCTCA TGGTCTGCCC TTCCCTGGGG CAATAGCTAG GGTCTTTCCT 2401 GATTTTTATG GAATTTTAGG GGATATTTTG AGCTTTGGGT TCTCAGTAGT 2451 GAATTGAGAC TTGGAGGTGA CTTTTCATGT TTGGAGTATC ATCTCTGTCT 2501 GGGCTCTGGG CTGACAAATT AAAACCTAGA GTAGTGCTTA TGCTGAAATG 2551 ATACTTTTCA TTTTTTGGTT GATTTTTTTG CCTTCCCTTC AATTTTAAAC 2601 TGAAGCATTT TAATGTGGGT AGAAACTCTA CACCAAATAC ACTAAACATT 2651 TTGGTGCTTA GTGGATTTCT TTTTAGGTAA CTGGTACTTA CTTCCAAAGA 2701 CTGAATACAA GCCACACTCC ATCATATCCC TTAAACTTCA TGAAAAACCA 2751 TTCAAGATCC CCTTGCTGCA ACACTGTTCT CTTCTTCTCT ACTAAATTCT 2801 ATTTCCAAAA TTGGTAATAG AGCCAGAAGG ATCCCCAGTA CCCAGCCCTC 2851 TGCCTGGCAC AAAGTGGTAG CACAATTAAA TTCAGTATGG GTGGAGCATG 2901 GTACAGTCTT GGTGCCATAG AAGGAGTAGT TGCATAGTCA CACATCATTT 2951 GATAAGTTGG ATGTTCCATT ACATAGAGGA ACACAAAATT CCAGGGTTTT 3001 TGGAGGAAGG GATTAGATAG CGACTAAGCC GCCAGAATTG AGGTGGCCAT 3051 TCCTTTTTGT ATAGGCTAAG AAACAGGTTA TCAGTGAAAA GTTAATTATG 3101 GCTTTGGCAC TAGAATAGCA CTGTTGCAAA GTATTTAAGC ACCCCCCATC 3151 TCAGCCCTTT ATTTTATCTT TCATGTGGGC TAATGTGAGG ATAATCTTAC 3201 AGATATTATA GGAATTTCTT TTCTATCTTT ATGAAAACAA CGTATATAAA 3251 ATATATCTAG AAAACCTTTG TTTGAGACTC TTATTTAATG GGCTTTTGAT 3301 TCTAATGATA ATTGTACCTT TATCTTTCAA AAGCTGATAT TTCCTACCTA 3351 AGCATCTCCC GAGAAAAATA TCTCATTAAA AAGCCCATAA ATAATAGGGG 3401 AGAAGAAAGC CTTAGGTATC AATTCCAAAA CAGTGATTGA AATTTCCCAA 3451 AATAATTATG GCTTCTGTCA TCTCCAGAGA TAATCTGGCT TGGTTTACCC 3501 CATAATCTAA TTTCAGAAAA GAAAGCTTTA TTTTAACACT CATCTGAATC 3551 AACATTAAAG CCTTTTCTCT CAAAGCGTTT ATTGAGAAAC TCAAATGAAT 3601 ATACTTTTTG AATTACTGTC ATCAAAAGTG TACGGCTTCC TGTGCTGCTT 3651 GTGTCAAATG GAACCTGCCC TCTAAAGCAC TTTCTTTCCT TTACTTGCGT 3701 GGTTTCATGT AAGCTGTGCT GTTTAGAAAC AACATCTCAG ACTTTACAAA 3751 GAAATGACAA AGAAGGCAAT TGCACTTTTT AAGGGATATC GACAAGCAGT 3801 TTCTGTTTTC TAAAGGACAA AATACAGAGT GTGTGTCATT TTTAATTAGA 3851 TTCTTTCCCC TGCTGAGTTG GAAATTCCAG TGCAGCACTG ATTGACCACA 3901 GTTGCCAATC TAAAAGCACA AAGACAGAAG TAAAGCTTTA TGCTAATTTT 3951 ATTTCAATAT GATAGAAAAT TTATCTTGGT ATGTCCTTTT TTAGATAACT 4001 CCAGCAGGAA ACTGTAACTG CTATGTCTTT AGGAAAACGT AGAAGAAAGA 4051 ACATTATTAT TCTTTAATTC CTACAAGGTA CTTGAAAACC TTAAGTGAAA 4101 AAGATTTCTA TCTTTTTATC TTGGCGCATT TATGGAAAAA ATATTAACTG 4151 TCCTGAATAT TTTATAATTT TGTAGGAAAA ATATGCATCT ATTTTTTCTT 4201 GACTTCTTTT ATATAGTAAT AAAAGTTATT TTGGAAAAAA AAAAAAAAAA 4251 AAAA

BLAST Results

Entry HSG20626 from database EMBL: human STS A005Z27. Score = 860, P = 3.0e-32, identities = 176/181

Medline entries

89030633:

The structure of cytochrome b561, a secretory vesicle-specific electron transport protein.

Peptide information for frame 2

ORF from 74 bp to 931 bp; peptide length: 286 Category: strong similarity to known protein Classification: unset

1 MAMEGYRRFL ALLGSALLVG FLSVIFALVW VLHYREGLGW DGΞALEFNWH 51 PVLMVTGFVF IQGIAIIVYR LPWTWKCSKL LMKSIHAGLN AVAAILAIIS 101 VVAVFENHNV NNIANMYSLH SWVGLIAVIC YLLQLLSGFS VFLLPWAPLS 151 LRAFLMPIHV YSGIVIFGTV IATALMGLTE KLIFSLRDPA YSTFPPEGVF 201 VNTLGLLILV FGALIFWIVT RPQWKRPKEP NSTILHPNGG TEQGARGSMP 251 AYSGNNMDKS DSELNNEVAA RKRNLALDEA GQRSTM

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_7e22, frame 2 SWISSPROT:C561 SHEEP CYTOCHROME B561 (CYTOCHROME B-561)., N = 1, Score = 460, P = 1.3e-43

PIR:S01167 cytochrome b561 - bovine, N = 1, Score = 457, P = 2.7e-43

SWISSPROT:C561_PIG CYTOCHROME B561 (CYTOCHROME B-561)., N = 1, Score 452, P = 9.1e-43

PIR:S53321 cytochrome B561 - human, N = 1, Score = 451, P = 1.2e-42

>SWISSPROT:C561_SHEEP CYTOCHROME B561 (CYTOCHROME B-561) Length = 252

HSPs:

Score = 460 (69.0 bits), Expect = 1.3e-43, P = 1.3e-43 Identities = 96/218 (44%), Positives = 131/218 (60%)

Query 18 LVGFLSVIFALVWVLHYREGLGWDGSALEFNWHPVLMVTGFVFIQGIAIIVYRLPWTWKC 77

L+G V W+ YR G+ W+ SAL+FN HP+ MV G VF+QG A++VYR+ Sbjct 23 LLGLTVVAMTGAWLGMYRGGIAWE-SALQFNVHPLCMVIGLVFLQGDALLVYRV—FRNE 79 Query 78 SKLLMKSIHAGLNAVAAILAIISVVAVFENHNVNNIANMYSLHSWVGLIAVICYLLQLLS 137

+K K +H L+ A ++A++ +VAVFE+H A++YSLHSW G++ + Q L Sbjct 80 AKRTTKVLHGLLHVFAFVIALVGLVAVFEHHRKKGYADLYSLHSWCGILVFALFFAQWLV 139 Query 138 GFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTEKLIFSLRDPAYSTFPPE 197

GFΞ FL P A SLR+ P HV+ G IF +ATAL+GL E L+F L YSTF PE Sbjct 140 GFSFFLFPGASFSLRSRYRPQHVFFGAAIFLLSVATALLGLKEALLFEL-GTKYSTFEPE 198 Query 198 GVFVNTLGLLILVFGALIFWIVTRPQWKRPKEPNSTIL 235 GV N LGLL+ F ++ +I+TR WKRP + L Sbjct 199 GVLANVLGLLLAAFATVVLYILTRADWKRPLQAEEQAL 236

Pedant information for DKFZphfbr2_7e22, frame 2

Report for DKFZphfbr2_7e22.2

[LENGTH] 286

[MW] 31638.58

[pi] 9.12

[HOMOL] SWISSPROT :C561_SHEEP CYTOCHROME B561 (CYTOCHROME B-561) . 4e-40

[PIRKW] transmembrane protein 9e-40

[KW] SIGNAL_PEPTIDE 40

[KW] TRANSMEMBRANE 5

[KW] LOW COMPLEXITY 4.90 %

SEQ MAMEGYRRFLALLGSALLVGFLSVIFALVWVLHYREGLGWDGSALEFNWHPVLMVTGFVF

SEG

PRD ccchhhhhhhhhhhhhhhhhhhhcchhhhhhhhhccccccccccccccccchhhhhhhhh

MEM MMMMMMMMMMMM

SEQ IQGIAIIVYRLPWTWKCSKLLMKSIHAGLNAVAAILAIISVVAVFENHNVNNIANMYSLH

SEG xxxxxxxxxxxxxx

PRD ccccceeeeeccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccceeecc

MEM MMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMMM

SEQ SWVGLIAVICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTE SEG PRD cccchhhhhhhhhhhhhhheeeeccccccccccccccceeeeeeeeeeehhhhhhhhhhh

MEM ....MMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMM ...

SEQ KLIFSLRDPAYSTFPPEGVFVNTLGLLILVFGALIFWIVTRPQWKRPKEPNSTILHPNGG SEG PRD hhhhhhhccccccccccchhhhhhhhhhhhhhhheeeeeecccccccccccccccccccc

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ TEQGARGSMPAYSGNNMDKSDSELNNEVAARKRNLALDEAGQRSTM SEG PRD cccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhcccc MEM

(No Prosite data available for DKFZphfbr2_7e22.2) (No Pfam data available for DKFZphfbr2 7e22.2) DKFZphfbr2_7j4

group: brain derived

DKFZphfbr2_7 4 encodes a novel 233 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes . unknown complete cDNA, complete eds, 1 EST h t

Sequenced by GBF

Locus : unknown

Insert length: 1050 bp

Poly A stretch at pos. 1027, polyadenylation signal at pos. 1007

1 GGGGACACAA AGGGGTGGTC ACCCTGCCCT CACCTTGACC TGTAAGTTGC 51 CTAGGACAGT GGCCTGGTCC CAGGGGCTGT TGTGGGGAGT TGAAGAACAC 101 CCTGGCCTCC TCCATCATGT CGGCCAAGAG GGCAGAATTG AAGAAAACAC 151 ATCTGTGCAA GAACTACAAG GCAGTTTGCC TGGAATTGAA GCCAGAGCCG 201 ACCAAAACAT TTGATTACAA AGCAGTTAAA CAAGAAGGGC GGTTTACCAA 251 AGCAGGAGTG ACACAGGACC TAAAGAATGA ACTCAGGGAA GTGAGAGAAG 301 AGCTCAAGGA GAAAATGGAG GAGATAAAAC AGATAAAGGA TCTAATGGAC 351 AAGGATTTTG ATAAACTTCA CGAATTTGTG GAAATTATGA AGGAAATGCA 401 GAAAGATATG GATGAGAAGA TGGACATTTT AATAAATACA CAGAAGAACT 451 ATAAGCTTCC CCTTAGAAGA GCACCAAAGG AGCAGCAGGA ACTCAGGCTG 501 ATGGGAAAGA CTCACAGAGA ACCACAGCTC AGGCCCAAGA AAATGGATGG 551 AGCCAGTGGA GTCAATGGAG CACCCTGTGC TCTTCACAAG AAGACGATGG 601 CACCACAAAA AACAAAACAG GGCTCACTGG ATCCCCTTCA TCACTGTGGG 651 ACCTGCTGCG AGAAATGTTT GTTGTGTGCT CTAAAGAACA ACTACAATCG 701 GGGGAACATT CCTTCAGAGG CCTCAGGCCT TTACAAAGGT GGAGAGGAGC 751 CAGTGACCAC CCAACCTTCT GTGGGCCACG CTGTGCCTGC CCCAAAGTCC 801 CAGACTGAGG GAAGGTGAAG CTTAACTGCC AGCTTGAAAT GAGAGTAAAG 851 AAGATACAGA GCAAACAGTG TTTCAGAAAC TGTCCTGCCC TGGGTGTGAT 901 TCTTTGGCTT CAATTTGAAG GAGGAGGAAT GATGGGATTT CATATTTTAT 951 TTCACACCAG TTCCTCCTTG TTTCATCTCT TTGCTAAGCT GGCTGCTTCT 1001 ACCATCTAAT AAATAATTGG CCAAGTTAAA AAAAAAAAAA AAAAAAAAAA

BLAST Results No BLAST result

Medl e entries No Medline entry

Peptide information for frame 3

ORF from 117 bp to 815 bp; peptide length: 233 Category: putative protein

1 MSAKRAELKK THLCKNYKAV CLELKPEPTK TFDYKAVKQE GRFTKAGVTQ

51 DLKNELREVR EELKEKMEEI KQIKDLMDKD FDKLHEFVEI MKEMQKDMDE

101 KMDILINTQK NYKLPLRRAP KEQQELRLMG KTHREPQLRP KKMDGASGVN

151 GAPCALHKKT MAPQKTKQGS LDPLHHCGTC CEKCLLCALK NNYNRGNIPS

201 EASGLYKGGE EPVTTQPΞVG HAVPAPKSQT EGR

BLASTP hits

Entry JC2223 from database PIR: major surface glycoprotein 3 - Pneumocystis carinii (fragment)

Score = 109, P = 3.5e-04, identities = 41/136, positives = 67/136 Alert BLASTP hits for DKFZphfbr2_7j4, frame 3

TREMBLNEW: PCP115C_1 product: "P115C"; Pneumocystis carinii mRNA for P115C, partial sequence., N = 1, Score = 109, P = 0.00024

>TREMBLNEW:PCP115C_1 product: "P115C"; Pneumocystis carmii mRNA for P115C, partial sequence.

Length = 196

HSPs:

Score = 109 (16.4 bits), Expect = 2.4e-04, P = 2.4e-04 Identities = 41/134 (30%), Positives = 67/134 (50%)

Query: 14 CKN-YKAVCLELKPEPTKTFDYKAVKQEGRFTKA-GVTQDLKNELREVREELKEKMEEIK 71

CK K C ELK + K VK+ TK G ++LK+++++ E KE++E K Sbjct: 22 CKTELKKYCEELKEADGLKVNDK-VKEICDDTKRDGKCKELKDKVKKELETFKEELE—K 78 Query: 72 QIKDLMDKDFDKLHEFVEIMKEMQKDMDEKMDILINTQKNYKLPLRRAPKEQQELRLMGK 131

+KD+ D++ +K E +++E D D K + + + YKL +R E LR +GK Sbjct: 79 ALKDIKDENCEKYEEKCILLEETNHD-DVKKNCVKLREGCYKLKRKRVA-EDLLLRALGK 136 Query: 132 THREPQLRPKKMDGAS 147

+ + K D S Sbjct: 137 DVKNGECEKKMKDVCS 152

Pedant information for DKFZphfbr2_7j4, frame 3

Report for DKFZphfbr2_7j4.3

[LENGTH] 233

[MW] 26533.95

[pi] 9.18

[PROSITE] MYRISTYL 3

[PROSITE] CK2 PHOSPHO SITE 3

[PROSITE] PKC PHOSPHO SITE 3

[KW] All Alpha

[KW] LOW COMPLEXITY 14.59 %

[KW] COILED COIL 13.73 %

SEQ MSAKRAELKKTHLCKNYKAVCLELKPEPTKTFDYKAVKQEGRFTKAGVTQDLKNELREVR SEG xxxxxxxxx PRD ccchhhhhhhhhhccchhhhhhhcccccccccccceeecccccccccccchhhhhhhhhh COILS CCCCCCCCCCCC

SEQ EELKEKMEEIKQIKDLMDKDFDKLHEFVEIMKEMQKDMDEKMDILINTQKNYKLPLRRAP SEG xxxxxxxx xxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhcchhhhhhhhhhhhhhhhhhchhhhhhhhhcccccccccccc COILS CCCCCCCCCCCCCCCCCCCC

SEQ KEQQELRLMGKTHREPQLRPKKMDGASGVNGAPCALHKKTMAPQKTKQGSLDPLHHCGTC SEG PRD hhhhhhhhhccccccccccccccccccccccccchhhhhhcccccccccccccccccccc COILS

SEQ CEKCLLCALKNNYNRGNIPSEASGLYKGGEEPVTTQPSVGHAVPAPKSQTEGR SEG PRD chhhhhhhccccccccccccccccccccccccccccccccccccccccccccc COILS

Prosite for DKFZphfbr2_7j4.3

PS00005 2->5 PKC_PHOSPHO_SITE PDOC00005 PS00005 108->111 PKC_PHOSPHO_SITE PDOC00005 PS00005 132->135 PKC_PHOSPHO_SITE PDOC00005 PS00006 132->136 CK2_PHOSPHO_SITE PDOC00006 PS00006 179->183 CK2_PHOΞPHO_SITE PDOC00006 PS00006 228->232 CK2_PHOSPHO_SITE PDOC00006 PS00008 151->157 MYRISTYL PDOC00008 PS00008 196->202 MYRISTYL PDOC00008 PΞ00008 204->210 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_7j4.3) DKFZphfbr2_82c20

group: transmembrane protein

DKFZphfbr2_82c20 encodes a novel 492 amino acid protein with very weak similarity to C. elegans cosmid D1007.

The novel protein contains 7 transmembrane regions .

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes and as a new marker for neuronal cells. similarity to C. elegans D1007.5 ; membrane regions : 7

Summary DKFZphfbr2_82c20 encodes a novel 492 ammo acid protein with similarity to a hypothetical C. elegans protein. similarity to C. elegans D1007.5 complete cDNA (Bp 1-100 GC ritch) , complete eds, potential start at Bp 128 matches Kozak consensus PyNNatgG, EST hits, localisation? primer B of STS doesn't match perfect! TRANSMEMBRANE 7

Sequenced by DKFZ

Locus: /map="109.9 cR from top of Chrl linkage group"???

Insert length: 1804 bp

Poly A stretch at pos. 1794, no polyadenylation signal found

1 CGGCGGGAGC GCGCGGCTGA TACCCGGGAC TGGGCTGCGG CGGTTAGTCC

51 TCTCCCGGCC GCCGTCGCCT CCGACATATT GCTCGCAGGA GCTGCGGCGG

101 CGAAGCGGAG AGCACCGGGG GGAGGAGATG GGAGGACGAA GAGGTCCCAA

151 CAGGACATCT TACTGTCGAA ATCCGCTCTG TGAGCCGGGA TCCTCGGGGG

201 GCTCTAGTGG AAGCCACACT TCCAGTGCAT CGGTGACCAG TGTTCGTTCC

251 CGCACCAGGA GCAGTTCTGG AACAGGCCTC TCCAGCCCTC CTCTGGCCAC

301 CCAAACTGTT GTGCCTCTAC AGCACTGCAA GATCCCCGAG CTGCCAGTCC

351 AGGCCAGCAT TCTGTTTGAG TTGCAGCTCT TCTTCTGCCA GCTCATAGCA

401 CTCTTCGTCC ACTACATCAA CATCTACAAG ACAGTGTGGT GGTATCCACC

451 TTCCCACCCA CCCTCCCACA CCTCCCTGAA CTTCCATCTG ATCGACTTCA

501 ACTTGCTGAT GGTGACCACC ATCGTTCTGG GCCGCCGCTT CATTGGGTCC

551 ATCGTGAAGG AGGCCTCTCA GAGGGGGAAG GTCTCCCTCT TTCGCTCCAT

601 CCTGCTGTTC CTCACTCGCT TCACCGTTCT CACGGCAACA GGCTGGAGTC

651 TGTGCCGATC CCTCATCCAC CTCTTCAGGA CCTACTCCTT CCTGAACCTC

701 CTGTTCCTCT GCTATCCGTT TGGGATGTAC ATTCCGTTCC TGCAGCTGAA

751 TTGCGACCTC CGCAAGACAA GCCTCTTCAA CCACATGGCC TCCATGGGGC

801 CCCGGGAGGC GGTCAGTGGC CTGGCAAAGA GCCGGGACTA CCTCCTGACA

851 CTGCGGGAGA CGTGGAAGCA GCACACAAGA CAGCTGTATG GCCCGGACGC

901 CATGCCCACC CATGCCTGCT GCCTGTCACC CAGCCTCATC CGCAGTGAGG

951 TGGAGTTCCT CAAGATGGAC TTCAACTGGC GCATGAAGGA AGTGCTCGTC

1001 AGCTCCATGC TGAGCGCCTA CTATGTGGCC TTTGTGCCTG TCTGGTTCGT

1051 GAAGAACACA CATTACTATG ACAAGCGCTG GTCCTGTGAA CTCTTCCTGC

1101 TGGTGTCCAT CAGCACCTCC GTGATCCTCA TGCAGCACCT GCTGCCTGCC

1151 AGCTACTGTG ACCTGCTGCA CAAGGCCGCC GCCCATCTGG GCTGTTGGCA

1201 GAAGGTGGAC CCAGCGCTGT GCTCCAACGT GCTGCAGCAC CCGTGGACTG

1251 AAGAATGCAT GTGGCCGCAG GGCGTGCTGG TGAAGCACAG CAAGAACGTC

1301 TACAAAGCCG TAGGCCACTA CAACGTGGCT ATCCCCTCTG ACGTCTCCCA

1351 CTTCCGCTTC CATTTCTTTT TCAGCAAACC TCTGCGGATC CTCAACATCC

1401 TCCTGCTGCT GGAGGGCGCT GTCATTGTCT ATCAGCTGTA CTCCCTAATG

1451 TCCTCTGAAA AGTGGCACCA GACCATCTCG CTGGCCCTCA TCCTCTTCAG

1501 CAACTACTAT GCCTTCTTCA AGCTGCTCCG GGACCGCTTG GTATTGGGCA

1551 AGGCCTACTC ATACTCTGCT AGCCCCCAGA GAGACCTGGA CCACCGTTTC

1601 TCCTGAGCCC TGGGGTCACC TCAGGGACAG CGTCCAGGCT TCAGCCAAGG

1651 GCTCCCTGGC AAGGGGCTGT TGGGTAGAAG TGGTGGTGGG GGGGACAAAA

1701 GACAAAAAAA TCCACCAGAG CTTTGTATTT TTGTTACGTA CTGTTTCTTT

1751 GATAATTGAT GTGATAAGGA AAAAAGTCCT ATTTTTATAC TCCCAAAAAA

1801 AAAA

BLAST Results

Entry HS285343 from database EMBL: human STS WI-17488. Score = 1225, P = 1.3e-50, identities = 263/281

Medline entries

No Medline entry

Peptide information for frame 2

1 MGGRRGPNRT SYCRNPLCEP GSSGGSSGSH TSSASVTSVR SRTRSSSGTG 51 LSSPPLATQT VVPLQHCKIP ELPVQAΞILF ELQLFFCQLI ALFVHYINIY 101 KTVWWYPPSH PPSHTSLNFH LIDFNLLMVT TIVLGRRFIG SIVKEASQRG 151 KVSLFRSILL FLTRFTVLTA TGWSLCRSLI HLFRTYΞFLN LLFLCYPFGM 201 YIPFLQLNCD LRKTSLFNHM ASMGPREAVS GLAKSRDYLL TLRETWKQHT 251 RQLYGPDAMP THACCLSPSL IRSEVEFLKM DFNWRMKEVL VSSMLSAYYV 301 AFVPVWFVKN THYYDKRWSC ELFLLVSIST SVILMQHLLP ASYCDLLHKA 351 AAHLGCWQKV DPALCSNVLQ HPWTEECMWP QGVLVKHSKN VYKAVGHYNV 401 AIPSDVSHFR FHFFFSKPLR ILNILLLLEG AVIVYQLYSL MSSEKWHQTI 451 SLALILFSNY YAFFKLLRDR LVLGKAYSYS ASPQRDLDHR FS

ORF from 128 bp to 1603 bp; peptide length: 492 Category: similarity to unknown protein Prosite motifs: LEUCINE_ZIPPER (210-232) LEUCINE ZIPPER (210-232)

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_82c20, frame 2

TREMBL :CEAF3151_8 gene: "D1007.5"; Caenorhabditis elegans cosmid D1007., N = 2, Score = 247, P = 4.6e-29

>TREMBL:CEAF3151_8 gene: "D1007.5"; Caenorhabditis elegans cosmid D1007.

Length = 512

HSPs:

Score = 247 (37.1 bits), Expect = 4.6e-29, Sum P(2) = 4.6e-29 Identities = 58/204 (28%), Positives = 102/204 (50%)

Query: 291 VSSMLSAYYVAFVPVWFVKNTHYYDKRWSCELFLLVSISTSVILMQHLLPAΞYCDLLHKA 350

+S ML +V F + ++ W C+L ++V ++ + + +L P +Y DLLH+A Sbjct: 299 LSIMLPCIFVPFKTSQGIPQKILINEVWECQLAIVVGLTAFSLYVAYLSPLNYLDLLHRA 358

Query: 351 AAHLGCWQKVD-PAL CSNVLQHPWTEECMWPQGVLVKHSKN-VYKAVGHYNV 400

A HLG W +++ P + + PW+E C++ G V+ Y+A ++ Sbjct: 359 AIHLGSWHQIEGPRIGHTGSMSSAPTPWΞEFCLYNDGETVQMPDGRCYRAKSSNSIRTVA 418

Query: 401 AIPSDVSHFRFHFFFSKPLRILNILLLLEGAVIVYQLYSLMSSEKWHQTISLALILFSNY 460

A P H F KP ++NI+ E +1 Q + L+ + W ++ L++F+NY Sbjct: 419 AHPESSRHNTFFKVLRKPNNLINIMCSFEFLLIFIQFWMLVLTNDWQHIVTFVLLMFANY 478

Query: 461 YAFFKLLRDRLVLGKAYΞYSASPQRDL 487

F KL +D+++L + Y S Q DL Sbjct: 479 LLFAKLFKDKIILSRIYEPS QEDL 502

Score = 178 (26.7 bits), Expect = 4.3e-21, Sum P(2) = 4.3e-21 Identities = 50/179 (27%), Positives = 90/179 (50%)

Query: 262 HACCLSPSLIRSEVEFLKMDFNWRMKEVLVSSMLSAYYVAFVPVWFV—KNTHYYDKR-- 317

H C SP+ IR E++ L D R+K+ + + + +A+ +P FV K + ++ Sbjct: 262 HMCSDSPAQIREEIQVLIDDLVLRVKKSIFAGVSTAFLSIMLPCIFVPFKTSQGIPQKIL 321

Query: 318 WSCELFLLVSISTSVILMQHLLPASYCDLLHKAAAHLGCWQKVD-PAL CSNV 368

W C+L ++V ++ + + +L P +Y DLLH+AA HLG W +++ P + + Sbjct: 322 INEVWECQLAIVVGLTAFSLYVAYLSPLNYLDLLHRAAIHLGSWHQIEGPRIGHTGSMSS 381

Query: 369 LQHPWTEECMWPQGVLVKHSKN-VYKAVGHYNV-AIPSDVSHFRFHFFFSKPLRILNILL 426

PW+E C++ G V+ Y+A ++ + + R + FF K LR N L+ Sbjct: 382 APTPWSEFCLYNDGETVQMPDGRCYRAKSSNSIRTVAAHPESSRHNTFF-KVLRKPNNLI 440 Score = 146 (21.9 bits), Expect = 4.6e-29, Sum P(2) = 4.6e-29 Identities = 34/86 (39%), Positives = 50/86 (58%)

Query: 52 SSPPLATQTVVPLQHCKIPELP-VQASILFELQLFFCQLIALFVHYINIYKTVWWYPPSH 110

+S P A+ + + H P++ Q + FE LF ++ALF+ Y+NIYKT+WW P S+ Sbjct: 19 ASIPRASGVTLSV-HPIWPDIQFTQGELFFECTLFLYSVLALFLQYLNIYKTLWWLPKSY 77

Query: 111 PPSHTSLNFHLIDFNLLMVTTIVLGRR 137

H SL FHLI+ L ++LG R Sbjct: 78 --WHYSLKFHLINPYFLSCVGLLLGWR 102

Score = 39 (5.9 bits), Expect = 6.8e-18, Sum P(2) = 6.8e-18 Identities = 12/41 (29%), Positives = 20/41 (48%)

Query: 154 LFRSILLFLTRFTVLTATGWSLCRSLIHLFRTYΞFLNLLFL 194

L+ + LFL ++ + T W L +S H + +N FL Sbjct: 53 LYSVLALFL-QYLNIYKTLWWLPKSYWHYSLKFHLINPYFL 92

Pedant information for DKFZphfbr2_82c20, frame 2

Report for DKFZphfbr2_82c20.2

[LENGTH] 492 [MW] 56274.05 [pl] 9.51 [HOMOL] TREMB :CEAF3151 8 gene: "D1007.5"; Caenorhabditis elegans cosmid D1007. 4e-31

[PROSITE] LEUCINE_ZIPPER 1

[PROSITE] AMIDATION 2

[PROSITE] MYRISTYL 5

[PROSITE] CAMP_PHOSPHO_SITE 2

[PROSITE] CK2_PHOSPHO_SITE 3

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE 5

[PROSITE] ASN_GLYCOSYLATION 1

[KW] TRANSMEMBRANE 7

[KW] LOW COMPLEXITY 8.74

SEQ MGGRRGPNRTSYCRNPLCEPGSSGGSSGSHTSSASVTSVRSRTRSSSGTGLSSPPLATQT SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD ccccccccccccccccccccccccccccccccccccceeeccccccccccccccccccee

MEM

SEQ VVPLQHCKIPELPVQASILFELQLFFCQLIALFVHYINIYKTVWWYPPSHPPSHTSLNFH

SEG

PRD eeeccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhheeeeeccccccccceeeeee

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM MMMMMMMMM

SEQ LIDFNLLMVTTIVLGRRFIGSIVKEASQRGKVΞLFRSILLFLTRFTVLTATGWSLCRSLI

SEG

PRD eeehhhhhhhhhhhhheeeehhhhhhhcccchhhhhhhhhhhhhhhhhhcccchhhhhhh

MEM MMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ HLFRTYSFLNLLFLCYPFGMYIPFLQLNCDLRKTSLFNHMASMGPREAVSGLAKSRDYLL

SEG

PRD hhhhhhhhheeeeeeecccccceeeeccccchhhhhhhhhhccchhhhhhhhhhhhhhhh

MEM

SEQ TLRETWKQHTRQLYGPDAMPTHACCLSPSLIRSEVEFLKMDFNWRMKEVLVSSMLSAYYV

SEG

PRD hhhhhhhhhhhhccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhcchhhhhh

MEM MMMMMMMMMMMMMMMMM

SEQ AFVPVWFVKNTHYYDKRWSCELFLLVSISTSVILMQHLLPASYCDLLHKAAAHLGCWQKV

SEG

PRD heeeeeeeeccccccchhhhhhhhhhhcchhhhhhhhhhccchhhhhhhhhhhhhhhccc

MEM MMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ DPALCSNVLQHPWTEECMWPQGVLVKHSKNVYKAVGHYNVAIPSDVSHFRFHFFFSKPLR

SEG xx

PRD ccccccccccccccceeecccceeeeeccceeeeccccccccccccccceeeeeecccch

MEM MMMMMMMMMM

SEQ ILNILLLLEGAVIVYQLYSLMSSEKWHQTISLALILFSNYYAFFKLLRDRLVLGKAYSYS

SEG xxxxxxxx

PRD hhhhhhhhhhheeeeehhhhhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccc

MEM MMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMM SEQ ASPQRDLDHRFS

SEG

PRD ccchhhhhhccc

MEM

Prosite for DKFZphfbr2_82c20.2

PS00001 8->12 ASN_GLYCOSYLATION PDOC00001 PS00002 47->51 GLYCOSAMINOGLYCAN PDOC00002 PS00004 212->216 CAMP_PHOSPHO_SITE PDOC00004 PS00004 316->320 CAMP_PHOSPHO_SITE PDOC00004 PS00005 38->41 PKC_PHOΞPHO_SITE PDOC00005 PS00005 147->150 PKC_PHOSPHO_SITE PDOC00005 PS00005 241->244 PKC_PHOSPHO_SITE PDOC00005 PS00005 245->248 PKC_PHOSPHO_SITE PDOC00005 PS00005 443->446 PKC_PHOSPHO_SITE PDOC00005 PS00006 241->245 CK2_PHOSPHO_SITE PDOC00006 PS00006 273->277 CK2_PHOSPHO_SITE PDOC00006 PS00006 342->346 CK2_PHOSPHO_SITE PDOC00006 PS00008 21->27 MYRISTYL PDOC00008 PS00008 24->30 MYRISTYL PDOC00008 PS00008 28->34 MYRISTYL PDOC00008 PS00008 48->54 MYRISTYL PDOC00008 PS00008 231->237 MYRISTYL PDOC00008 PS00009 2->6 AMIDATION PDOC00009 PS00009 134->138 AMIDATION PDOC00009 PS00029 168->190 LEUCINE ZIPPER PDOC00029

(No Pfam data available for DKFZphfbr2_82c20.2)

DKFZphfbr2 82el7

group: transmembrane protein

DKFZphfbr2_82el7 encodes a novel 311 amino acid protein with very weak similarity to C. elegans cosmid R01B10.

The novel protein contains 6 transmembrane regions.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application n studying the expression profile of brain-specific genes and as a new marker for neuronal cells.

similarity to C. elegans "R01B10.5" ; membrane regions: 6

Summary DKFZphfbr2_82el7 encodes a novel 311 amino acid protein with similarity to a hypothetical C. elegans protein. similarity to C. elegans "R01B10.5" cDNA, EST HS763158 extendes the sequence, complete eds, EST hits six potential transmembrane domains

Sequenced by DKFZ

Locus: /map="779_C_?; 818_A_1; 877_C_1; 734_C_12; 760_E_11; 171.7 cR from top of Chrl4 linkage group"

Insert length: 1618 bp

Poly A stretch at pos. 1608, polyadenylation signal at pos. 1588

1 CTGATCTAGT GCTTCTCGAA AAAAACCTTC AGGCGGCCCA TGGCTGTCGA

51 TATTCAACCA GCATGCCTTG GACTTTATTG TGGGAAGACC CTATTATTTA

101 AAAATGGCTC AACTGAAATA TATGGAGAAT GTGGGGTATG CCCAAGAGGA

151 CAGAGAACGA ATGCACAGAA ATATTGTCAG CCTTGCACAG AATCTCCTGA

201 ACTTTATGAT TGGCTCTATC TTGGATTTAT GGCAATGCTT CCTCTGGTTT

251 TACATTGGTT CTTCATTGAA TGGTACTCGG GGAAAAAGAG TTCCAGCGCA

301 CTTTTCCAAC ACATCACTGC ATTATTTGAA TGCAGCATGG CAGCTATTAT

351 CACCTTACTT GTGAGTGATC CAGTTGGTGT TCTTTATATT CGTTCATGTC

401 GAGTATTGAT GCTTTCTGAC TGGTACACGA TGCTTTACAA CCCAAGTCCA

451 GATTACGTTA CCACAGTACA CTGTACTCAT GAAGCCGTCT ACCCACTATA

501 TACCATTGTA TTTATCTATT ACGCATTCTG CTTGGTATTA ATGATGCTGC

551 TCCGACCTCT TCTGGTGAAG AAGATTGCAT GTGGGTTAGG GAAATCTGAT

601 CGATTTAAAA GTATTTATGC TGCACTTTAC TTCTTCCCAA TTTTAACCGT

651 GCTTCAGGCA GTTGGTGGAG GCCTTTTATA TTACGCCTTC CCATACATTA

701 TATTAGTGTT ATCTTTGGTT ACTCTGGCTG TGTACATGTC TGCTTCTGAA

751 ATAGAGAACT GCTATGATCT TCTGGTCAGA AAGAAAAGAC TTATTGTTCT

801 CTTCAGCCAC TGGTTACTTC ATGCCTATGG AATAATCTCC ATTTCCAGAG

851 TGGATAAACT TGAGCAAGAT TTGCCCCTTT TGGCTTTGGT ACCTACACCA

901 GCCCTTTTTT ACTTGTTCAC TGCAAAATTT ACCGAACCTT CAAGGATACT

951 CTCAGAAGGA GCCAATGGAC ACTGAGTGTA GACATGTGAA ATGCCAAAAA

1001 CCTGAGAAGT GCTCCTAATA AAAAAGTAAA TCAATCTTAA CAGTGTATGA

1051 GAACTATTCT ATCATATATG GGAACAAGAT TGTCAGTATA TCTTAATGTT

1101 TGGGTTTGTC TTTGTTTTGT TTATGGTTAG ACTTACAGAC TTGGAAAATG

1151 CAAAACTCTG TAATACTCTG TTACACAGGG TAATATTATC TGCTACACTG

1201 GAAGGCCGCT AGGAAGCCCT TGCTTCTCTC AACAGTTCAG CTGTTCTTTA

1251 GGGCAAAATC ATGTTTCTGT GTACCTAGCA ATGTGTTCCC ATTTTATTAA

1301 GAAAAGCTTT AACACGTGTA ATCTGCAGTC CTTAACAGTG GCGTAATTGT

1351 ACGTACCTGT TGTGTTTCAG TTTGTTTTTC ACCTATAATG AATTGTAAAA

1401 ACAAACATAC TTGTGGGGTC TGATAGCAAA CATAGAAATG ATGTATATTG

1451 TTTTTTGTTA TCTATTTATT TTCATCAATA CAGTATTTTG ATGTATTGCA

1501 AAAATAGATA ATAATTTATA TAACAGGTTT TCTGTTTATA GATTGGTTCA

1551 AGATTTGTTT GGATTATTGT TCCTGTAAAG AAAACAATAA TAAAAAGCTT

1601 ACCTACATAA AAAAAAAA

BLAST Results

Entry HS981146 from database EMBL: human STS WI-6253.

Length = 208

Minus Strand HSPs:

Score = 1040 (156.0 bits), Expect = 1.9e-40, 1.9e-40 Identities = 208/208 (100%), Positives = 208/208 (100%), Strand = Minus / Plus

Entry HSG20716 from database EMBL: human STS A006D06.

Length = 195

Minus Strand HSPs:

Score = 975 (146.3 bits), Expect = 1.8e-37, P = 1.8e-37

Identities = 195/195 (100%), Positives = 195/195 (100%), Strand = Minus

/ Plus

Medline entries

No Medline entry

Peptide information for frame 1

1 MAVDIQPACL GLYCGKTLLF KNGSTEIYGE CGVCPRGQRT NAQKYCQPCT

51 ESPELYDWLY LGFMAMLPLV LHWFFIEWYS GKKSSSALFQ HITALFECSM

101 AAIITLLVSD PVGVLYIRSC RVLMLSDWYT MLYNPSPDYV TTVHCTHEAV

151 YPLYTIVFIY YAFCLVLMML LRPLLVKKIA CGLGKSDRFK SIYAALYFFP

201 ILTVLQAVGG GLLYYAFPYI ILVLSLVTLA VYMSASEIEN CYDLLVRKKR

251 LIVLFSHWLL HAYGIISISR VDKLEQDLPL LALVPTPALF YLFTAKFTEP

301 SRILSEGANG H

ORF from 40 bp to 972 bp; peptide length: 311 Category: similarity to unknown protein

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_82el7, frame 1

TREMBL:AF068718_5 gene: "R01B10.5"; Caenorhabditis elegans cosmid R01B10., N = 1, Score = 399, P = 1.4e-36

>TREMBL:AF068718_5 gene: "R01B10.5"; Caenorhabditis elegans cosmid R01B10. Length = 670

HSPs:

Score = 399 (59.9 bits), Expect = 1.4e-36, P = 1.4e-36 Identities = 95/280 (33%), Positives = 152/280 (54%)

Query: 2 AVDIQPACLGLYCGKTLLFKN GSTEIYGECGVCPRGQRTNAQKYCQPC 49

A IQP+CLG +CG+T+L N GST + CG C G R NA C+ C

Sbjct: 292 ASTIQPSCLG-FCGRTVLVGNYSEDVEATTTAAGSTSL-SRCGPCSFGYRNNAMSICESC 349

Query: 50 TESPELYDWLYLGFMAMLPLVLHWFFIEWYSGKKSSSALFQ HITALFECSMAAIITL 106

+ YDW+YL F+A+LPL+LH FI + K + ++ ++ + E +A +1 + Sbjct: 350 DTPLQPYDWMYLLFIALLPLLLHMQFIR-IARKYCRTRYYEVSEYLCVILENVIACVIAV 408

Query: 107 LVSDPVGVLYIRSCRVLMLSDWYTMLYNPSPDYVTTVHCTHEAVYPLYTIVFIYYAFCLV 166

L+ P ++ C + +WY YNP Y T+ CT+E V+PLY+I FI++ + Sbjct: 409 LIYPPRFTFFLNGCSKTDIKEWYPACYNPRIGYTKTMRCTYEVVFPLYSITFIHHLILIG 468

Query: 167 LMMLLRPLLVKKIACGLGKSDRFKSIYAALYFFPILTVLQAVGGGLLYYAFPYIILVLSL 226

+++LR L + L K+ K YAA+ PIL V+ AV G+++Y FPYI+L+ SL Sbjct: 469 SILVLRSTLYCVL LYKTYNGKPFYAAIVSVPILAVIHAVLSGVVFYTFPYILLIGSL 525

Query: 227 VTLAVYMSASEIENCYDLLVR KKRLIVLFSHWLLHAYGIISI 268

+ +++ +++VR LI L L+ ++G+I+I Sbjct: 526 WAMCFHLALEGKRPLKEMIVRIATSPTHLIFLSITMLMLΞFGVIAI 571

Pedant information for DKFZphfbr2_82el7, frame 1

Report for DKFZphfbr2_82el7.1 [LENGTH] 311 [MW] 35239.14

[pi] 7.91 [HOMOL] TREMBL:AF068718_5 gene: "R01B10.5"; Caenorhabditis elegans cosmid R01B10. 9e-36

[PROSITE] AMIDATION 1

[PROSITE] MYRISTYL 3

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 3

[PROSITE] PKC_PHOSPHO_SITE 4

[PROSITE] ASN_GLYCOSYLATION 1

[KW] TRANSMEMBRANE 6

[KW] LOW_COMPLEXITY 7.72 %

SEQ MAVDIQPACLGLYCGKTLLFKNGSTEIYGECGVCPRGQRTNAQKYCQPCTESPELYDWLY SEG PRD cccccccccccccccceeeeccccceeecccccccccccccceeecccccccccchhhhh MEM MMMMMM

SEQ LGFMAMLPLVLHWFFIEWYSGKKSSSALFQHITALFECSMAAIITLLVSDPVGVLYIRSC SEG PRD hhhhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhccccceeeeeece MEM MMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM...

SEQ RVLMLSDWYTMLYNPSPDYVTTVHCTHEAVYPLYTIVFIYYAFCLVLMMLLRPLLVKKIA

SEG xxxxxxxxxxxx ....

PRD eeeeecceeeeecccccceeeeeeeceeeeeeeeceeeeehhhhhhhhhhhhhhhhhhee

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM...

SEQ CGLGKSDRFKSIYAALYFFPILTVLQAVGGGLLYYAFPYIILVLSLVTLAVYMSASEIEN SEG PRD eecccccchhhhhhhhhhhccccccccccccceeeecceeeeehhhhhhhhhhhhhhhhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ CYDLLVRKKRLIVLFSHWLLHAYGIISISRVDKLEQDLPLLALVPTPALFYLFTAKFTEP

SEG xxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhhhcccceeeechhhhhhceeeeeecccceeeeeeeccccc

MEM MMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMM

SEQ SRILSEGANGH SEG PRD ceeeeeccccc MEM MM

Prosite for DKFZphfbr2_82el7.1

PS00001 22->26 ASN_GLYCOSYLATION PDOC00001 PS00004 82->86 CAMP_PHOSPHO_SITE PDOC00004 PS00005 80->83 PKC_PHOSPHO_SITE PDOC00005 PS00005 119->122 PKC_PHOSPHO_SITE PDOC00005 PS00005 186->189 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 294->297 PKC_PHOSPHO_SITE PDOC00005 PS00006 234->238 CK2_PHOSPHO_SITE PDOC00006 PS00006 236->240 CK2_PHOSPHO_SITE PDOC00006 PS00006 269->273 CK2_PHOSPHO_SITE PDOC00006 PS00008 11->17 MYRISTYL PDOC00008 PS00008 37->43 MYRISTYL PDOC00008 PS00008 182->188 MYRISTYL PDOC00008 PS00009 80->84 AMIDATION PDOC00009

(No Pfam data available for DKFZphfbr2_82el7.1) DKFZphfbr2_82e4

group: signal transduction

DKFZphfbr2_82e4 encodes a novel 473 amino acid protein with strong similarity to the calmodulin-bmding proteins.

The novel protein is similar to human and rat Ca2+/calmodulιn-dependent protein kinase (EC 2.7.1.123), rat calmodulin-bmding protein, calmodulin binding protein kinase of Fugu rupies and Rattus norvegicus calcium/calmodulm-dependent protein kinase I. Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. Calmodulin is involved m regulation of growth and cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters. The novel protein seems to be involved in calmodulm-mediated pathways m human neuronal cells.

The new protein can find clinical application m modulating/blocking calmodulin-mediated pathways m human neuronal cells . strong similarity to calmodulin-bmding proteins complete cDNA, complete eds, EST hits splice variant in comparison to rat 156542 ESTs HSZZ54543/HS1141907 define splice variant see also DKFZphfbr2_82g20 unspliced form

Sequenced by DKFZ

Locus: /map="200.5 cR from top of Chr3 linkage group"

Insert length: 2923 bp

Poly A stretch at pos. 2913, polyadenylation signal at pos. 2890

1 ATGCTGGAGG TTCGCTAGCC GAAGCGGCTG CATCTGGCGC CGCGTCTGCC 51 CCGCGTGCTC GGAGCGGATT CTGCCCGCCG TCCCCGGAGC CCTCGGCGCC

101 CCGCTGAGCC CGCGATCACT TCCTCCCTGT GACCAACCGG CGCTGCAGGT

151 TAGAGCCTGG CAATGCCGTT TGGGTGTGTG ACTCTGGGTG ACAAGAAGAA

201 CTATAACCAG CCATCGGAGG TGACTGACAG ATATGATTTG GGACAGGTCA

251 TCAAGACTGA GGAGTTTTGT GAAATCTTCC GGGCCAAGGA CAAGACGACA

301 GGCAAGCTGC ACACCTGCAA GAAGTTCCAG AAGCGGGACG GCCGCAAGGT

351 GCGGAAAGCT GCCAAGAACG AGATAGGCAT CCTCAAGATG GTGAAGCATC

401 CCAACATCCT ACAGCTGGTG GATGTGTTTG TGACCCGCAA GGAGTACTTT

451 ATCTTCCTGG AGCTGGCCAC GGGGAGGGAG GTGTTTGACT GGATCCTGGA

501 CCAGGGCTAC TACTCGGAGC GAGACACAAG CAACGTGGTA CGGCAAGTCC

551 TGGAGGCCGT GGCCTATTTG CACTCACTCA AGATCGTGCA CAGGAATCTC

601 AAGCTGGAGA ACCTGGTTTA CTACAACCGG CTGAAGAACT CGAAGATTGT

651 CATCAGTGAC TTCCATCTGG CTAAGCTAGA AAATGGCCTC ATCAAGGAGC

701 CCTGTGGGAC CCCCGAGTAT CTGGGCAACC CACCTTTCTA TGAGGAGGTG

751 GAAGAAGATG ATTATGAGAA CCATGATAAG AATCTCTTCC GCAAGATCCT

801 GGCTGGTGAC TATGAGTTTG ACTCTCCATA TTGGGATGAT ATTTCGCAGG

851 CAGCCAAAGA CCTGGTCACA AGGCTGATGG AGGTGGAGCA AGACCAGCGG

901 ATCACTGCAG AAGAGGCCAT CTCCCATGAG TGGATTTCTG GCAATGCTGC

951 TTCTGATAAG AACATCAAGG ATGGTGTCTG TGCCCAGATT GAAAAGAACT 1001 TTGCCAGGGC CAAGTGGAAG AAGGCTGTCC GAGTGACCAC CCTCATGAAA 1051 CGGCTCCGGG CACCAGAGCA GTCCAGCACG GCTGCAGCCC AGTCGGCCTC 1101 AGCCACAGAC ACTGCCACCC CCGGGGCTGC AGGTGGGGCC ACAGCTGCAG 1151 CTGCGAGTGG AGCTACCTCA GCCCCTGAGG GTGATGCTGC TCGTGCTGCA 1201 AAGAGTGATA ATGTGGCCCC CGCAGACCGT AGTGCCACCC CAGCCACAGA 1251 TGGAAGTGCC ACCCCAGCCA CTGATGGCAG TGTCACCCCA GCCACCGATG 1301 GAAGCATCAC TCCAGCCACT GATGGGAGTG TCACCCCAGC CACTGACAGG 1351 AGCGCTACTC CAGCCACTGA TGGGAGAGCC ACACCAGCCA CAGAAGAGAG 1401 CACTGTGCCC ACCACCCAAA GCAGTGCCAT GCTGGCCACC AAGGCAGCTG 1451 CCACCCCTGA GCCGGCTATG GCCCAGCCGG ACAGCACAGC CCCAGAGGGC 1501 GCCACAGGCC AGGCTCCACC CTCTAGTAAA GGGGAAGAGG CTGCTGGTTA 1551 TGCCCAGGAG TCTCAAAGGG AGGAGGCCAG CTGAGTAGGC AGCCTGGTGA 1601 GGGGGGGCAG GGGATGGGCA GGAGGGTGGG AGAGTGGATG AGGGGCTTCT 1651 CACTGTACAT AGAGTCACTG GCATGATGCC CTCGCTCCCC CATGCCCCCA 1701 CATCCCAGTG GGGCATAACT AGGGGTCACG GGAGAGCAGT CTCGTCTCCT 1751 GTGTGTATGT GTGTGAGTGG TGGGCAGGCC AGTGGCAGGG CCGGCCCCAG 1801 CCCCTGCATG GATTCCTTGT GGCTTTTCTG TCTTTTGCTA GCTTCACCAG 1851 TTTCTGTTCC TTGTGGGATG CTGCTCTAGG GATACTCAGG GGGCTCCTGC 1901 TCTCCTTCCC CTTCCCTTCT TGCCTCACCA TTCCCCTAGG CAGGCCCTGC 1951 AGGTCCCACA CTCTCCCAGG CCCTAAACTT GGGCGGCCTT GCCCTGAGAG 2001 CTGGTCCTCC AGCGAGGCCC TGTCAGCGGT CTTAGGCTCC TGCACATGAA 2051 GGTGTGTGCC TGTGGTGTGT GGGCTGCTCT AGGAGCAGAT ACAGGCTGGT 2101 ATAGAGGATG CAGAAAGGTA GGGCAGTATG TTTAAGTCCA GACTTGGCAC 2151 ATGGCTAGGG ATACTGCTCA CTAGCTGTGG AGGTCCTCAG GAGTGGAGAG 2201 AATGAGTAGG AGGGCAGAAG CTTCCATTTT TGTCCTTCCT AAGACCCTGT 2251 TATTTGTGTT ATTTCCTGCC TTTCCGAGTC CTGCAGTGGG CTGCCCTGTA

2301 CCCTGAACCT CATGAGCCTC TAAGGGAAAG GAGGAACAAT TAGGACGTGG

2351 CAATGAGACC TGGCAGGGCA GAGTACAAGC CCAGCACCCA GTGTCCCAGC

2401 CTTACTGGGT CCTTACCCTG GGCCAAACAG GGAGGGCTGA TACCTCCTTG

2451 CTCTTCCTAG ATGCCCACCT CCTACAATCT CAGCCCACAA GTCCTCTCCA

2501 CCCTAGGGGG CTTGCTGCAT GGCAATAACT CATAATCTGA TTTGGAGGTT

2551 TGCCCTTTAC AGGGGCAGAT TTTCTGCTCA GTTCAACAAT GAAATGAAGA

2601 GGAACTCCCT CTTTCTACAG CTCACTTCTA TCAGAGGCCC AGGTGCCTCA

2651 GAGCCACATT GAGTTGCTTT TTCTGGGATG AGGAAGTAGG GTTAAACTCC

2701 CCAGTTTCCT GAGGGAGGCT CCTGACAGGT GCCCTTTGTC AGACCCTACC

2751 ACAGCCTGGA TAGGCAGCCA CATTGGTCCT CGCCCTTGCT CGGCACTCCG

2801 TGGTGGTCCT GCCCTTCTCC CTGCATGCCT GTGGGTCTGC TCTGGTGTGT

2851 GAAGGTCGGT GGGTTAACTG TGTGCCTACT GAACCTGGCA AATAAACATC

2901 ACCCTGCAAA GCCAAAAAAA AAA

BLAST Results

Entry HS452352 from database EMBL: human STS WI-15318.

Length = 350

Minus Strand HSPs:

Score = 1547 (232.1 bits), Expect = 5.2e-63, P = 5.2e-63

Identities = 331/348 (95%), Positives = 331/348 (95%), Strand = Minus /

PI

Medline entries

94110847:

J Neurosci 1994 Jan; 14 ( 1) : 1-13

1G5 : a calmodulin-bmdmg, vesicle-associated, protein kinase-like protein enriched in forebrain neuπtes.

Godbout M, Erlander MG, Hasel KW, Danielson PE, Wong KK, Battenberg EL,

Foye PE,

Bloom FE, Sutcliffe JG

Peptide information for frame 1

1 MPFGCVTLGD KKNYNQPSEV TDRYDLGQVI KTEEFCEIFR AKDKTTGKLH

51 TCKKFQKRDG RKVRKAAKNE IGILKMVKHP NILQLVDVFV TRKEYFIFLE

101 LATGREVFDW ILDQGYYSER DTSNVVRQVL EAVAYLHSLK IVHRNLKLEN

151 LVYYNRLKNS KIVISDFHLA KLENGLIKEP CGTPEYLGNP PFYEEVEEDD

201 YENHDKNLFR KILAGDYEFD SPYWDDISQA AKDLVTRLME VEQDQRITAE

251 EAISHEWISG NAASDKNIKD GVCAQIEKNF ARAKWKKAVR VTTLMKRLRA

301 PEQSSTAAAQ SASATDTATP GAAGGATAAA ASGATSAPEG DAARAAKSDN

351 VAPADRSATP ATDGSATPAT DGSVTPATDG SITPATDGSV TPATDRSATP

401 ATDGRATPAT EESTVPTTQS SAMLATKAAA TPEPAMAQPD STAPEGATGQ

451 APPSSKGEEA AGYAQESQRE EAS

ORF from 163 bp to 1581 bp; peptide length: 473 Category: strong similarity to known protein

BLASTP hits

Entry S50193 from database PIR:

Ca2+/calmodulιn-dependent protein kinase (EC 2.7.1.123) I - rat

Length = 374

Score = 371 (130.6 bits), Expect = 2.2e-66, Sum P(2) = 2.2e-66

Identities = 74/176 (42%), Positives = 115/176 (65%)

Entry S57347 from database PIR:

Ca2+/calmodulm-dependent protein kinase (EC 2.7.1.123) I - human

Length = 370

Score = 369 (129.9 bits), Expect = 4.6e-66, Sum P(2) = 4.6e-66

Identities = 74/176 (42%), Positives = 114/176 (64%)

Alert BLASTP hits for DKFZphfbr2_82e4, frame 1

PIR: 156542 calmodulin-bmdmg protein - rat, N = 2, Score = 1246, P 4e-228 TREMBLNEW: FRU010348_3 product: "calmodulin binding protein kinase"; Fugu rubripes UBEl-like gene, PRGFR2 gene and gene encoding calmodulin binding protein kinase, clone 168J21, N = 2, Score = 846, P = 2.6e-139

TREMBL :RNPRKI_1 product: "protein kinase I"; Rattus norvegicus calcium/calmodulm-dependent protein kinase I mRNA, complete eds., N = 2, Score = 364, P = 5. le-63

>PIR: 156542 calmodulm-binding protein Length = 504

HSPs:

Score = 1246 (186.9 bits), Expect = 4.0e-228, Sum P(2) 4.0e-228 Identities = 255/289 (88%), Positives = 259/289 (89%)

Query: 188 GNPPFYEEVEEDDYENHDKNLFRKILAGDYEFDSPYWDDISQAAKDLVTRLMEVEQDQRI 247

GNPPFYEEVEEDDYENHDKNLFRKILAGDYEFDSPYWDDISQAAKDLVTRLMEVEQDQRI Sbjct: 216 GNPPFYEEVEEDDYENHDKNLFRKILAGDYEFDSPYWDDISQAAKDLVTRLMEVEQDQRI 275

Query: 248 TAEEAISHEWISGNAASDKNIKDGVCAQIEKNFARAKWKKAVRVTTLMKRLRAPEQSSTA 307

TAEEAISHEWISGNAASDKNIKDGVCAQIEKNFARAKWKKAVRVTTLMKRLRAPEQS TA Sbjct: 276 TAEEAISHEWISGNAASDKNIKDGVCAQIEKNFARAKWKKAVRVTTLMKRLRAPEQSGTA 335

Query: 308 AAQSASATDTATPGAAGGATAAAASGATSAPE GDAARAAKSDNVAPADRSAT 359

A +D ATPGAAGGA AAAA GA A GDA AAKSD++A ADRSAT

Sbjct: 336 AT SDAATPGAAGGAVAAAAGGAAPASGASATVGTGGDAGCAAKSDDMASADRSAT 390

Query: 360 PATDGSATPATDGSVTPATDGSITPATDGSVTPATDRSATPATDGRATPATEESTVPTTQ 419

PATDGSATPATDGSVTPATDGSITPATDGSVTPATDRSATPATDGRATPATEESTVP Q Sbjct: 391 PATDGSATPATDGSVTPATDGSITPATDGSVTPATDRSATPATDGRATPATEESTVPAAQ 450

Query: 420 SSAMLATKAAATPEPAMAQPDSTAPEGATGQAPPSSKGEEAAGYAQESQREEAS 473

SSA A KAAATPEPA+AQPDSTA EGATGQAPPSSKGEEA G AQESQR E S Sbjct: 451 SSAAPAAKAAATPEPAVAQPDSTALEGATGQAPPSSKGEEATGCAQESQRVETS 504

Score = 978 (146.7 bits), Expect = 4.0e-228, Sum P(2) = 4.0e-228 Identities = 186/187 (99%), Positives = 187/187 (100%)

Query: 1 MPFGCVTLGDKKNYNQPSEVTDRYDLGQVIKTEEFCEIFRAKDKTTGKLHTCKKFQKRDG 60

MPFGCVTLGDKKNYNQPSEVTDRYDLGQV+KTEEFCEIFRAKDKTTGKLHTCKKFQKRDG Sbjct: 1 MPFGCVTLGDKKNYNQPSEVTDRYDLGQVVKTEEFCEIFRAKDKTTGKLHTCKKFQKRDG 60

Query: 61 RKVRKAAKNEIGILKMVKHPNILQLVDVFVTRKEYFIFLELATGREVFDWILDQGYYSER 120

RKVRKAAKNEIGILKMVKHPNILQLVDVFVTRKEYFIFLELATGREVFDWILDQGYYSER Sbjct: 61 RKVRKAAKNEIGILKMVKHPNILQLVDVFVTRKEYFIFLELATGREVFDWILDQGYYSER 120

Query: 121 DTSNVVRQVLEAVAYLHSLKIVHRNLKLENLVYYNRLKNSKIVISDFHLAKLENGLIKEP 180

DTSNVVRQVLEAVAYLHSLKIVHRNLKLENLVYYNRLKNSKIVISDFHLAKLENGLIKEP Sbjct: 121 DTSNVVRQVLEAVAYLHSLKIVHRNLKLENLVYYNRLKNSKIVISDFHLAKLENGLIKEP 180

Query: 181 CGTPEYL 187

CGTPEYL Sbjct: 181 CGTPEYL 187

Pedant information for DKFZphfbr2_82e4, frame 1

Report for DKFZphfbr2_82e4.1

[LENGTH] 473

[MW] 5120 8.89

[pi] 5.30

[HOMOL] PIR: 156542 calmodulin-bindmg protein - rat 0.0

[FUNCAT] 30.0 3 organization of cytoplasm [S. cerevisiae, YFR014c] 4e-30

[FUNCAT] 10.9 9 other signal-transduction activities [S. cerevisiae, YFR014c] 4e-30

[FUNCAT] 03.0 1 cell growth [S. cerevisiae, YFR014c] 4e-30

[FUNCAT] 30.1 0 nuclear organization [S. cerevisiae, YKLlOlw] 2e-26

[FUNCAT] 0₃.2 2 cell cycle control and mitosis [S. cerevisiae, YKLlOlw] 2e-26

[FUNCAT] 11.0 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YDLlOlc] 8e-26

[FUNCAT] 98 class fication not yet clear-cut [S. cerevisiae, YCL024w] 5e-24

[FUNCAT] 03.2 5 cytokinesis [S. cerevisiae, YDR507c] 7e-23

[FUNCAT] 03.0 4 budding, cell polarity and filament formation [S. cerevisiae, YDR507c]

7e-23

[FUNCAT] 03.22.01 cell cycle check point proteins [S. cerevisiae, YPL153c] le-21

[FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YPL153c] le-21 [FUNCAT] 11.01 stress response [S. cerevisiae, YDR477w] 3e-19

[FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YDR477w]

3e-19

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YPL141c] le-16

[FUNCAT] 03. 16 dna synthesis and replication [S. cerevisiae, YMROOlc] 3e-16

[FUNCAT] 03. 13 meiosis [S. cerevisiae, YOR351c] le-15

[FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YDR122w] 3e-14

[ FUNCA ] 10.03.11 key kinases [S. cerevisiae, YCR073c] 6e-ll

[FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YNR031c] 8e-ll

[FUNCAT] 10.02.11 key kinases [S. cerevisiae, YJL095w] 2e-09

[FUNCAT] 03.07 pheromone response, matmg-type determination, sex-specific proteins

[S. cerevisiae, YLR362w] le-08 [FUNCAT] 10.05.11 key kinases [S. cerevisiae, YLR362w] le-08 [FUNCAT] 10.04.11 key kinases [S. cerevisiae, YLR362w] le-08 [FUNCAT] 02.19 metabolism of energy reserves (glycogen, trehalose) [S. cerevisiae, YPL031c] 7e-08 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YPL031c] 7e-08 [FUNCAT] 01.04.04 regulation of phosphate utilization [S. cerevisiae, YPL031c] 7e-08 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YFL033c] le-07 [FUNCAT] 04.99 other transcription activities [S. cerevisiae, YFL033c] le-07 [FUNCAT] 10.05.09 regulation of g-protem activity [S. cerevisiae, YBL016w] 5e-07 [FUNCAT] 05.07 translational control [S. cerevisiae, YDR283c] 8e-07 [FUNCAT] 01.06.10 regulation of lipid, fatty-acid and sterol biosynthesis [S. cerevisiae, YHR079C] 5e-06 [FUNCAT] 30.07 organization of endoplasmatic reticulum [S. cerevisiae, YHR079C] 5e-06 [FUNCAT] 30.01 organization of cell wall [S. cerevisiae, YIR019c] le-05 [FUNCAT] 30.90 extracellular/secretion proteins [S. cerevisiae, YIR019c] le-05 [FUNCAT] 01.05.01 carbohydrate utilization [S. cerevisiae, YIR019c] le-05 [FUNCAT] 04.05.01.01 general transcription activities [S. cerevisiae, YDL108w] le-05 [FUNCAT] 01.02.04 regulation of nitrogen and sulphur utilization [Ξ. cerevisiae, YNL183C] 8e -05 [FUNCAT] 08.99 other intracellular-transport activities [S. cerevisiae, YNL183c] 8e-05 [FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YDR523C] 2e-04 [FUNCAT] c energy conversion [M. genitalium, MG109] 3e-04 [BLOCKS] BL00107A Protein kinases ATP-binding region proteins [BLOCKS] BL00939F [SCOP] dlgol 1 9 MAP kinase Erk2 [rat Rattus norvegicus 3e-62 [SCOP] dlwfc 1 8 MAP kinase p38 [human (Homo sapiens) 5e-59 [SCOP] dlkoa_2 1 7 (1-350) Twitchm, kinase domain [Caenorhabditi le-75 [SCOP] dlkoba_ 1 6 Twitchin, kinase domain [California sea har le-72 [SCOP] dlphk 1 5 gamma-subunit of glycogen phosphorylase kinas 4e-65 [SCOP] dlirk 2 4 insulin receptor [Human (Homo sapiens) 2e-56 [SCOP] dlapme_ 1 4 cAMP-dependent PK, catalytic subunit [mouse (Mu 4e-71 [SCOP] dlfgka_ 5.1.1 2 3 Fibroblast growth factor receptor 1 [human (Hom le-50 [SCOP] dlydre_ 5.1.1.1 3 cAMP-dependent PK, catalytic subunit [bovme (Bo 3e-70 [SCOP] dlfmk_3 5.1.1.2 2 (168-437) c-src tyrosine kinase [human (Hom 5e-49 [SCOP] dlcdkb_ 5.1.1.1.2 cAMP-dependent PK, catalytic subunit [pig (Su 2e-72 [SCOP] d2hcka3 5.1.1.2.1 (167-437) Haemopoetic cell kinase Hck [huma 5e-46 [SCOP] dlcsn 5.1.1.1.11 Casein kιnase-1, CK1 [Schizosaccharomyces pombe 9e-42 [SCOP] dljsua_ 5.1.1.1.1 Cyclin-dependent PK [Human (Homo sapiens) le-56 [SCOP] dlckιa_ 5.1.1.1.10 Casein kιnase-1, CK1 [rat (Rattus norvegicus) 9e-52 [EC] 2.7.1.38 Phosphorylase kinase 3e-29 [EC] 2.7.1.123 Ca2+/calmodulιn-dependent protein kinase 8e-66 [EC] 2.7.1.128 [Acetyl-CoA carboxylase] kinase 2e-17 [EC] 2.7.1.117 Myosm-light-chain kinase 2e-38 [EC] 2.7.1.109 [Hydroxymethylglutaryl-CoA reductase (NADPH) ] kinase 2e-17 [EC] 2.7.1.37 Protein kinase 6e-28 [PIRKW] phosphotransferase 8e-66 [PIRKW] nucleus 2e-24 [PIRKW] transferase 8e-30 [PIRKW] calcium 2e-27 [PIRKW] duplication 4e-19 [PIRKW] tandem repeat 2e-31 [PIRKW] phorbol ester binding le-16 [PIRKW] zinc le-16 [PIRKW] cell cycle control 2e-20 [PIRKW] serme/threonine-specific protein kinase 8e-66 [PIRKW] phospholipid binding le-16 [PIRKW] autophosphorylation 8e-66 [PIRKW] brain le-14 [PIRKW] heterotetramer 2e-16 [PIRKW] polymer 3e-29 [PIRKW] mitosis 2e-20 [PIRKW] magnesium le-22 [PIRKW] ATP 8e-66 [PIRKW] alternative initiators le-29 [PIRKW] phosphoprotem 8e-66

[PIRKW] apoptosis 2e-31

[PIRKW] glycoprotein 4e-19

[PIRKW] skeletal muscle 3e-28

[PIRKW] protein kinase 2e-28

[PIRKW] testis 3e-28

[PIRKW] signal transduction le-21

[PIRKW] cAMP binding le-16

[PIRKW] purine nucleotide binding 5e-25

[PIRKW] structural protein 4e-19

[PIRKW] calcium binding 3e-45

[PIRKW] alternative splicing 3e-45

[PIRKW] P-loop 5e-25

[PIRKW] lipoprotein 2e-16

[PIRKW] cardiac muscle 4e-19

[PIRKW] muscle 3e-28

[PIRKW] myristylation 2e-16

[PIRKW] EF hand 5e-29

[PIRKW] cell division 2e-38

[PIRKW] calmodulin binding 8e-66

[PIRKW] smooth muscle 7e-31

[SUPFAM] fibronectin type III repeat homology 7e-31

[SUPFAM] immunoglobulin homology 7e-31

[SUPFAM] ribosomal protein S6 kinase II 3e-26

[SUPFAM] calcium-dependent protein kinase 5e-29

[SUPFAM] AMP-activated protein k ase 7e-22

[SUPFAM] protein kinase akt le-14

[SUPFAM] protein kinase SPK1 3e-20

[SUPFAM] unassigned Ser/Thr or Tyr-specific protein kinases 2e-36

[SUPFAM] Ca2+/calmodulιn-dependent protein kmase 3e-45

[SUPFAM] calmodulin repeat homology 5e-29

[SUPFAM] protein kinase DUN1 2e-24

[SUPFAM] Dictyostelium cAMP-dependent protein kinase catalytic chain le-14

[SUPFAM] death-associated protein kmase 2e-31

[SUPFAM] myosm-light-chain kinase, nonmuscle le-29

[SUPFAM] pleckstπn repeat homology le-14

[SUPFAM] ankyrin repeat homology 2e-31

[SUPFAM] protein kinase homology 8e-66

[SUPFAM] Ca2+/calmodulm-dependent protein kinase II 8e-36

[SUPFAM] twitchin le-18

[SUPFAM] protein kinase C zinc-binding repeat homology le-16

[SUPFAM] titin 4e-19

[SUPFAM] protein kinase cdrl 2e-20

[SUPFAM] kinase-related transforming protein 2e-38

[SUPFAM] Ca2+/calmodulm-dependent protein kinase I 8e-66

[SUPFAM] kinase interaction domain homology 2e-24

[SUPFAM] protein kinase C mu le-16

[PROSITE] AMIDATION 1

[PROSITE] MYRISTYL 3

[PROSITE] CK2_PHOSPHO_SITE 10

[PROSITE] TYR_PHOSPHO_SITE 2

[PROSITE] PKC_PHOSPHO_SITE 11

[PFAM] Eukaryotic protein kinase domain

[KW] All_Alpha

[KW] 3D

[KW] LOW COMPLEXITY 7.40 %

SEQ MPFGCVTLGDKKNYNQPSEVTDRYDLGQVIKTEEFCEIFRAKDKTTGKLHTCKKFQKRDG SEG la06- CEETTTGGGCEEEEEECBCGGGGGEEEEEETTTTCEEEEEEEEC

SEQ RKVRKAAKNEIGILKMVKHPNILQLVDVFVTRKEYFIFLELATGREVFDWILDQGYYSER SEG la06- HHHHHHHHHCCTTTBCCEEEEEEETTEEEEEECCCCCEEHHHHHHHTTTTBHH

SEQ DTSNVVRQVLEAVAYLHSLKIVHRNLKLENLVYYNRLKNSKIVISDFHLAKLENGLIKEP SEG la06- HHHHHHHHHHHHHHHHHHHCCCTTTTTTTTEEECCCTTTTCEEECCCTTTTCHHHHHCCC

SEQ CGTPEYLGNPPFYEEVEEDDYENHDKNLFRKILAGDYEFDSPYWDDISQAAKDLVTRLME SEG la06- HHHHHHHCCTTTTTT THHHHHHHHHCCCCCCTTTTTTTTCHHHHHHHHHHCT

SEQ VEQDQRITAEEAISHEWISGNAASDKNIKDGVCAQIEKNFARAKWKKAVRVTTLMKRLRA SEG la06- TTGGGCCCHHHHHHTTTTTTCCCCCCBHHHHHHHHHHHHHCCTTTTTTBTHHHHHHHC ..

SEQ PEQSSTAAAQSASATDTATPGAAGGATAAAASGATSAPEGDAARAAKΞDNVAPADRSATP SEG ..xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx la06- SEQ ATDGSATPATDGSVTPATDGSITPATDGSVTPATDRSATPATDGRATPATEESTVPTTQS SEG la06-

SEQ SAMLATKAAATPEPAMAQPDSTAPEGATGQAPPSSKGEEAAGYAQESQREEAS SEG la06-

Prosite for DKFZphfbr2_82e4.1

PΞ00005 21->24 PKC PHOSPHO SITE PDOC00005

PS00005 46->49 PKC PHOSPHO^" -SITE PDOC00005

PS00005 51->54 PKC PHOSPHO^{" "}SITE PDOC00005

PS00005 91->94 PKC PHOSPHO SITE PDOC00005

PS00005 103->106 PKC PHOSPHO SITE PDOC00005

PS00005 118->121 PKC PHOSPHO^{" "}SITE PDOC00005

PS00005 138->141 PKC PHOSPHO- ^"SITE PDOC00005

PS00005 264->267 PKC PHOSPHO^{" "}SITE PDOC00005

PS00005 394->397 PKC PHOSPHO^{" "}SITE PDOC00005

PS00005 454->457 PKC PHOSPHO- SITE PDOC00005

PS00005 467->470 PKC PHOSPHO^{" "}SITE PDOC00005

PS00006 7->ll CK2 PHOSPHO SITE PDOC00006

PS00006 91->95 CK2 PHOSPHO SITE PDOC00006

PS00006 103-M07 CK2 PHOSPHO SITE PDOC00006

PS00006 118->122 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 248->252 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 313->317 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 336->340 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 442->446 CK2 PHOSPHO ^"SITE PDOC00006

PΞ00006 455->459 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 467->471 CK2 PHOSPHO ^"SITE PDOC00006

PS00007 456->464 TYR PHOSPHO ^"SITE PDOC00007

PS00007 1₂7->136 TYR PHOSPHO ^"SITE PDOC00007

PS00008 260->266 MYRISTYL PDOC00008

PS00008 321->327 MYRISTYL PDOC00008

PS00008 324->330 MYRISTYL PDOC00008

PS00009 59->63 AMIDATION PDOC00009

Pfam for DKFZphfbr2_82e4.1

HMM_NAME Eukaryotic protein kinase domain

HMM ^►YeigRiIGeGsFGtVYkCiWr.TGelVAIKIIkkrsms FlREIq

Y +G++I F ++++++++ TG++ K++ KR+ + +EI

Query 24 YDLGQVIKTEEFCEIFRAKDKTTGKLHTCKKFQKRDGRKVRKAAKNEIG 72

HMM IMRrLnHPNIIRFYDwFedddDHIYMIMEYMeGGDLFDYIrrngpMsEwe I+++++HPNI+++ D+F + +++ + +E++ G + FD+I ++G++SE++

Query 73 ILKMVKHPNILQLVDVFV-TRKEYFIFLELATGREVFDWILDQGYYSERD 121

HMM IrflMyQILrGMeYLHSMgllHRDLKPENILIDeN...gqIKIcDFGLAR ++++Q+L++++YLHS +I+HR LK EN+ + ++ I I+DF LA+

Query 122 TSNVVRQVLEAVAYLHSLKIVHRNLKLENLVYYNRLKNSKIVISDFHLAK 171

HMM qMnnYerMttfCGTPWY* + N ++ + CGTP+Y

Query 172 LEN—GLIKEPCGTPEY 186

HMM *GepPFyd dnMemlmrliqrfrrpfWpnCSeElyDFMr

G PPFY+ + +++I++++++F +P+W+ +S ++D+++

Query ιε GNPPFYEEVEEDDYENHDKNLFRKILAGDYEFDSPYWDDISQAAKDLVT 236

HMM wCWnyDPekRPTFrQILnHPWF* +++++ ++R+T+++++ H W+

Query 237 RLMEVEQDQRITAEEAISHEWI 258 DKFZphfbr2_82gl4

group: transmembrane protein

DKFZphfbr2_82gl4 encodes a novel 208 ammo acid proline-rich protein without similarity to known proteins.

The protein contains one transmembrane domain.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of bram-specific genes and as a new marker for neuronal cells. unknown prolm rich protein membrane regions : 1

Summary DKFZphfbr2_82gl4 encodes a novel 208 ammo acid protein. unknown prolin rich protein complete cDNA, complete eds, EST hits TRANSMEMBRANE 1

Sequenced by DKFZ

Locus: /map="26.2 cR from top of Chrl6 linkage group"

Insert length: 2059 bp

Poly A stretch at pos. 2049, polyadenylation signal at pos. 2024

1 AGAAGTGCGA CTGCCAGCTG CCGAGGCGTT CGGTCCTGCT GTTGCGGCCG

51 CTGCCCCAGG GCTGCGGGGA CGCTCCCGGA GCCCTGCCTG TCCCCTGTCC

101 ATCCAGGCCA GCAGCTGAAG GAGCCTCACC TGCCTCCCTT CTCTGAGTAG

151 CACGGATTTG AGGAGAAGCA GCGAAGATGT CCAGCGAGCC TCCCCCTCCT

201 TATCCTGGGG GCCCCACAGC CCCACTTCTG GAAGAGAAAA GTGGAGCCCC

251 GCCCACCCCA GGCCGTTCCT CCCCAGCTGT GATGCAGCCC CCTCCAGGCA

301 TGCCACTGCC CCCTGCGGAC ATTGGCCCCC CACCCTATGA GCCGCCGGGT

351 CACCCAATGC CCCAGCCTGG CTTCATCCCA CCACACATGA GTGCAGATGG

401 CACCTACATG CCTCCGGGTT TCTACCCTCC TCCAGGCCCC CACCCACCCA

451 TGGGCTACTA CCCCCCAGGG CCCTACACGC CAGGGCCCTA CCCTGGCCCT

501 GGGGGCCACA CAGCCACAGT CCTGGTCCCT TCAGGAGCTG CCACCACGGT

551 GACAGTGCTG CAGGGAGAGA TCTTTGAGGG AGCGCCTGTG CAGACGGTGT

601 GTCCCCACTG CCAGCAGGCC ATCGCCACCA AGATCTCCTA CGAGATTGGC

651 TTGATGAATT TCGTGCTGGG TTTCTTCTGT TGCTTCATGG GATGTGATCT

701 GGGCTGCTGC CTGATCCCCT GCCTCATCAA TGACTTCAAG GATGTGACGC

751 ACACATGCCC CAGCTGCAAA GCCTACATCT ACACGTACAA GCGCCTGTGC

801 TAACGGAGCT GGGACTCGGG ACTCCCCCGC CTGTCAGTCT GGCCCCCTGT

851 GCTTTGCTCC CTGCGCTCAG TGGTCACTTT CCCGCTCCCA CTTGGGGCTG

901 GGAGCCGTGC CACCATCCCC TAGAAGTCCT GTCCTCTTCA CCCTGCCCTA

951 CCTGAGCCGC TGACTCTTCT GGCAAAAATT CTGTTGGGAT TTAAGGCCAA

1001 GGGTCAGTGG GTGGCAGGGG GCTGGCAATG AGCTTGTGTG TTGTTGGTCT

1051 GCTTGGTGTG TGTGATCGGG AAGATAAGCT GGGAGGGGTC TCCTGCTGGG

1101 GTCCTGATGC CTCTGTTTCC AAACAAGGTA CAGGTTCAGT CCAGACTCTT

1151 TCCCCCTGGG ACCAACAGCA GCCAGAGCAG TTAGCCAGTT AGTCCCCAGG

1201 CCTGTGGCCA CAGGCGTTTC TGACCTGCTG GGCCGAGAAT GGGTAAGTTG

1251 TCTGGAGTCA GGTGGGCCCA CGTAGGACAG GGTCACAAAG CCTGGGTTTG

1301 TTTCTGGGTA CTTTGCGCCT CTGGGGTGCT AGAGGTGGGG CATGGTGGCT

1351 GGAAGTAAAA CTGCCAACTC TGGCCCTCAG AACTCTCAGG TATAGAAGCC

1401 CAGGATGTCT AATACCCTGT CCCAGTGCCC GAGAGCTGCC TGGTGTCAGG

1451 TAGAGAGGAC ACTGTACCTG GGTGAATGAT CAGACCCTGG TAGCTAAGAA

1501 GGAACTTGTC CCTTTGAGTC AGTGTGCAGA CCCCCTTTCA GGCCATGCCT

1551 CTGTGAACCC TGTATTGCTG GGGCCGGAAG GAGCCCCTGA GCCTAGCCCC

1601 TTCCCGTCTG CCCTGTGTCC TCACTGCGTG TGGGTATGAC CTCTGCCTGG

1651 TGGCTGGTGT ATCCCAACTG GGCAAGAGAT GGCAGAGGGT CCCCCTTGTG

1701 GGTGCGCTTG GATGTGCAGA GCCTTCTCCA TGGATTTTCT TCCCTGTAAG

1751 TGCCGGGCCC CCCACCCCAG CTGACAGGCT GTTGCTGTGC CTGCTCACAC

1801 CTGCTCCTGC AGGCACACTG GGCTAGGGAC GAGGAAGGAG CAGCCACAAG

1851 TGGTAGAACT GCCTTGGTGG ACACCAGCCT CGCCCTGTCT TTATTTCCTG

1901 AATGGTTTGT GAACTTGCTC ACCTGGACCA CTGTATCCTG CCACTGTCCT

1951 TCCTGGTCTC GCACTGCCAC TGCATGGCCT CCTGTCACTG TGAATCGTGG

2001 CCCAGTCTCA GTTTGTAGTT TCTCATTAAA TTGGCCCTTT CACTCCCCCA

2051 AAAAAAAAA

BLAST Results Entry HS727347 from database EMBL: human STS WI-16589.

Length = 275

Plus Strand HSPs:

Score = 1365 (204.8 bits), Expect = 3.0e-55, P = 3.0e-55

Identities = 275/276 (99%), Positives = 275/276 (99%), Strand = Plus /

PI

Medline entries

No Medlme entry

Peptide information for frame 3

1 MSSEPPPPYP GGPTAPLLEE KSGAPPTPGR SSPAVMQPPP GMPLPPADIG

51 PPPYEPPGHP MPQPGFIPPH MSADGTYMPP GFYPPPGPHP PMGYYPPGPY

101 TPGPYPGPGG HTATVLVPSG AATTVTVLQG EIFEGAPVQT VCPHCQQAIA

151 TKISYEIGLM NFVLGFFCCF MGCDLGCCLI PCLINDFKDV THTCPSCKAY

201 IYTYKRLC

ORF from 177 bp to 800 bp; peptide length: 208 Category: similarity to known protein

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_82gl4, frame 3

PIR:S57447 HPBRII-7 protein - human, N = 1, Score = 206, P = 8.4e-16

PIR:A47655 spliceosome-associated protein SAP 62 - human, N = 1, Score = 198, P = 4.3e-15

>PIR:S57447 HPBRII-7 protein - human Length = 551

HSPs:

Score = 206 (30.9 bits), Expect = 8.4e-16, P = 8.4e-16 Identities = 57/115 (49%), Positives = 62/115 (53%)

Query: 5 PPPPYPGGPTAPLLEEKSGAPPTPGRSSPAVMQPPPGMPLPPADIGPP PYEP 56

PPPP+P G T P G P PG P PPPG LPP GPP P P Sbjct: 226 PPPPFPAGQTPP--RPPLGPPGPPGPPGP PPPGQVLPPPLAGPPNRGDRPPPPVLF 279

Query: 57 PGHPMPQP—GFIPPHMSADGTYMP-PGFYPPPGPHPPM-GYYPP-GPYTPGPYPGPGGH 111

PG P QP G +PP G P PG+ PPPGP PP G PP GP+ P P PGP G Sbjct: 280 PGQPFGQPPLGPLPP GPPPPVPGYGPPPGPPPPQQGPPPPPGPFPPRP-PGPLGP 333

Query: 112 TATVLVP 118

T+ P Sbjct: 334 PLTLAPP 340

Score = 177 (26.6 bits), Expect = 1. le-12, P = 1. le-12 Identities = 55/120 (45%), Positives = 61/120 (50%)

Query: 5 PPPPYPGGPTAP—LLEEKSGAPPTPG-RSSPAVM QP PPGMPLPPADIGPPPYE 55

P PP P GP P +L PP G R P V+ QP PP PLPP GPPP Sbjct: 244 PGPPGPPGPPPPGQVLPPPLAGPPNRGDRPPPPVLFPGQPFGQPPLGPLPP GPPP-P 299

Query: 56 PPGHPMPQPGFIPPHMSADGTYMPPGFYPP--PGP-HPPMGYYPPGPYTPGPYPG PG 109

PG+ P PG PP G PPG +PP PGP PP+ PP P+ PGP PG P Sbjct: 300 VPGYG-PPPGPPPPQQ GPPPPPGPFPPRPPGPLGPPLTLAPP-PHLPGPPPGAPPPA 354

Query: 110 GHTATVLVP 118

H P Sbjct: 355 PHVNPAFFP 363

Score = 168 (25.2 bits), Expect = 1. le-11, P = 1. le-11 Identities = 47/118 (39%), Positives = 51/118 (43%)

Query: 5 PPPPYPG-GPTAPLLEEKSGAPPTPGRSSPAVMQP—PPGMPLPPADI-GPPPYEPPGHP 60 PPPP PG GP + G PP PG P P PP PP + GPPP PP P Sbjct: 296 PPPPVPGYGPPPGPPPPQQGPPPPPGPFPPRPPGPLGPPLTLAPPPHLPGPPPGAPPPAP 355

Query: 61 MPQPGFIPPHMSADGTYMPPGFYPPPGPHPPMGYYPPGPYTPGPYPGPGGHTATVLVPSG 120

P F PP ++ MP P P P G PP PY G Y PG T P Sbjct: 356 HVNPAFFPPPTNSG MPTSDSRGPPPTDPYGR-PP-PYDRGDYGPPGREMDTARTPLS 410

Query: 121 AA 122

A Sbjct: 411 EA 412

Score = 156 (23.4 bits), Expect = 2. le-10, P = 2. le-10 Identities = 44/103 (42%), Positives = 50/103 (48%)

Query: 6 PPPYPGGPTAPLLEEKSGAPPT-PGRSSPAVMQPPPGMPLPPADIGPPPYEPPGHPMPQP 64

P PGG P G PP P +P +PP G P PP GPPP PG +P P Sbjct: 208 PGAVPGGDRFPGPAGPGGPPPPFPAGQTPP—RPPLGPPGPPGPPGPPP PGQVLPPP 262

Query: 65 GFIPPHMSADGTYMPPGFYP-PPGPHPPMGYYPPGPYTP GPYPGP 108

PP+ D PP +P P PP+G PPGP P GP PGP Sbjct: 263 LAGPPNRG-DRP-PPPVLFPGQPFGQPPLGPLPPGPPPPVPGYGPPPGP 309

Score = 121 (18.2 bits), Expect = 5.2e-05, P = 5.2e-05 Identities = 40/90 (44%), Positives = 45/90 (50%)

Query: 23 GAPPTPGRSSPAVMQPP-PGMPLPPAD-IGPP-PYEPPGHPMPQPG-FIPPHMSADGTYM 78

G PG + P PP P PP +GPP P PPG P P PG +PP ++ Sbjct: 213 GGDRFPGPAGPGGPPPPFPAGQTPPRPPLGPPGPPGPPG-P-PPPGQVLPPPLAG 265

Query: 79 PP—GFYPPPG PHPPMGYYPPGPYTPGPYPG-PG 109

PP G PPP P P G P GP PGP P PG Sbjct: 266 PPNRGDRPPPPVLFPGQPFGQPPLGPLPPGPPPPVPG 302

Pedant information for DKFZphfbr2_82gl4, frame 3

Report for DKFZphfbr2_82gl4.3

[LENGTH] 208

[MW] 21862.47

[pi] 5.55

[PROSITE] MYRISTYL 3

[PROSITE] PKC_PHOSPHO_SITE 2

[KW] TRANSMEMBRANE 1

[KW] LOW_COMPLEXITY 39.90 %

SEQ MSSEPPPPYPGGPTAPLLEEKSGAPPTPGRSSPAVMQPPPGMPLPPADIGPPPYEPPGHP

SEG ....xxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD ccccccccccccccchhhhhhccccccccccccccccccccccccccccccccccccccc

MEM

SEQ MPQPGFIPPHMSADGTYMPPGFYPPPGPHPPMGYYPPGPYTPGPYPGPGGHTATVLVPSG

SEG xxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD ccccccccccccccccccccccccccccccccccccccccccccccccccceeeeecccc

MEM

SEQ AATTVTVLQGEIFEGAPVQTVCPHCQQAIATKISYEIGLMNFVLGFFCCFMGCDLGCCLI

SEG

PRD cceeeeeeeeeeecccceeeeccchhhhhhhhhhhhhhhceeeeeeeeeecccccceeec

MEM MMMMMMMMMMMMM

SEQ PCLINDFKDVTHTCPSCKAYIYTYKRLC

SEG .

PRD eeeecccccccccccccceeeeeeeccc

MEM MMMM

Prosite for DKFZphfbr2 82gl4.3

PS00005 196->199 PKC_PHOSPHO_SITE PDOC00005 PS00005 203->206 PKC_PHOSPHO_SITE PDOC00005 PS00008 109->115 MYRISTYL PDOC00008 PS00008 120->126 MYRISTYL PDOC00008 PΞ00008 172->178 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_82gl4.3) DKFZphfbr2_82ιl7

group: signal transduction

DKFZphtes2_82ιl7 encodes a novel 334 ammo acid protein with similarity to the plasma membrane substrate for the cAMP-dependent protein kinase.

The novel protein s a transmembrane protein with strong similarity to the phospholemman protein, a membrane substrate for the cAMP-dependent protein kinase. It seems to serve as a chloride channel or as a chloride-channel regulator.

The new protein can find application in modulating/blocking cAMP-dependent protein kinase- dependent pathways . similarity to plasma membrane substrate for cAMP-dependent protein kinase complete cDNA, complete eds, EST hits potential start at Bp 31 matches Kozak consensus PyNNatgG might be a SODIUM/POTASSIUM-TRANSPORTING ATPASE TRANSMEMBRANE 1

Sequenced by DKFZ

Locus: /map="ll; 920_E_12; 786_(A, H)_ll; (797, 802)_(E, H)_7"

Insert length: 1647 bp

Poly A stretch at pos. 1637, polyadenylation signal at pos. 1615

1 AGTCTCGGAG GGGACCGGCT GTGCAGACGC CATGGAGTTG GTGCTGGTCT 51 TCCTCTGCAG CCTGCTGGCC CCCATGGTCC TGGCCAGTGC AGCTGAAAAG

101 GAGAAGGAAA TGGACCCTTT TCATTATGAT TACCAGACCC TGAGGATTGG

151 GGGACTGGTG TTCGCTGTGG TTCTCTTCTC GGTTGGGATC CTCCTTATCC

201 TAAGTCGCAG GTGCAAGTGC AGTTTCAATC AGAAGCCCCG GGCCCCAGGA

251 GATGAGGAAG CCCAGGTGGA GAACCTCATC ACCGCCAATG CAACAGAGCC

301 CCAGAAAGCA GAGAACTGAA GTGCAGCCAT CAGGTGGAAG CCTCTGGAAC

351 CTGAGGCGGC TGCTTGAACC TTTGGATGCA AATGTCGATG CTTAAGAAAA

401 CCGGCCACTT CAGCAACAGC CCTTTCCCCA GGAGAAGCCA AGAACTTGTG

451 TGTCCCCCAC CCTATCCCCT CTAACACCAT TCCTCCACCT GATGATGCAA

501 CTAACACTTG CCTCCCCGCT GCAGCCTGTG GTCCTGCCCA CCTCCCGTGA

551 TGTGTGTGTG TGTGTGTGTG TGTGTGACTG TGTGTGTTTG CTAACTGTGG

601 TCTTTGTGGC TACTTGTTTG TGGATGGTAT TGTGTTTGTT AGTGAACTGT

651 GGACTCGCTT TCCCAGGCAG GGGCTGAGCC ACACGGCCAT CTGCTCCTCC

701 CTGCCCCCGT GGCCCTCCAT CACCTTCTGC TCCTAGGAGG CTGCTTGTTG

751 CCCGAGACCA GCCCCCTCCC CTGATTTAGG GATGCGTAGG GTAAGAGCAC

801 GGGCAGTGGT CTTCAGTCGT CTTGGGACCT GGGAAGGTTT GCAGCACTTT

851 GTCATCATTC TTCATGGACT CCTTTCACTC CTTTAACAAA AACCTTGCTT

901 CCTTATCCCA CCTGATCCCA GTCTGAAGGT CTCTTAGCAA CTGGAGATAC

951 AAAGCAAGGA GCTGGTGAGC CCAGCGTTGA CGTCAGGCAG GCTATGCCCT 1001 TCCGTGGTTA ATTTCTTCCC AGGGGCTTCC ACGAGGAGTC CCCATCTGCC 1051 CCGCCCCTTC ACAGAGCGCC CGGGGATTCC AGGCCCAGGG CTTCTACTCT 1101 GCCCCTGGGG AATGTGTCCC CTGCATATCT TCTCAGCAAT AACTCCATGG 1151 GCTCTGGGAC CCTACCCCTT CCAACCTTCC CTGCTTCTGA GACTTCAATC 1201 TACAGCCCAG CTCATCCAGA TGCAGACTAC AGTCCCTGCA ATTGGGTCTC 1251 TGGCAGGCAA TAGTTGAAGG ACTTCCTGTT CCGTTGGGGC CAGCACACCG 1301 GGATGGATGG AGGGAGAGCA GAGGCCTTTG CTTCTCTGCC TACGTCCCCT 1351 TAGATGGGCA GCAGAGGCAA CTCCCGCATC CTTTGCTCTG CCTGTCAGTG 1401 GTCAGAGCGG TGAGCGAGGT GGGTTGGAGA CTCAGCAGGC TCCGTGCAGC 1451 CCTTGGGAAC AGTGAGAGGT TGAAGGTCAT AACGAGAGTG GGAACTCAAC 1501 CCAGATCCCG CCCCTCCTGT CCTCTGTGTT CCCGCGGAAA CCAACCAAAC 1551 CGTGCGCTGT GACCCATTGC TGTTCTCTGT ATCGTGACCT ATCCTCAACA 1601 ACAACAGAAA AAAGGAATAA AATATCCTTT GTTTCCTAAA AAAAAAA

BLAST Results

Entry HS31455 from database EMBL: human STS WI-2739.

Length = 103

Minus Strand HSPs:

Score = 487 (73.1 bits), Expect = 4.4e-14, P = 4.4e-14

Identities = 101/104 (97%), Positives = 101/104 (97%), Strand = Minus /

Plus frame shift in primer binding site Medlme entries

91250422:

Purification and complete sequence determination of the major plasma membrane substrate for cAMP-dependent protein kinase and protein kinase C in myocardium.

95091702:

Protein kinase C and cyclic AMP-dependent protein kinase phosphorylate phospholemman, an insulin and adrenaline-regulated membrane phosphoprotem, at specific sites in the carboxy terminal domain.

95138184:

Mat-8, a novel phospholemman-like protein expressed in human breast tumors, induces a chloride conductance in Xenopus oocytes.

Peptide information for frame 2

1 MELVLVFLCS LLAPMVLASA AEKEKEMDPF HYDYQTLRIG GLVFAVVLFS 51 VGILLILSRR CKCSFNQKPR APGDEEAQVE NLITANATEP QKAEN

ORF from 32 bp to 316 bp; peptide length: 95 Category: strong similarity to known protein

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_82ιl7, frame 2

SWISSPROT :PLM_HUMAN PHOSPHOLEMMAN PRECURSOR., N = 1, Score = 196, P = 1.2e-15

TREMBL: AF091390_1 product: "phospholemman precursor"; Mus musculus phospholemman precursor, gene, complete eds., N = 1, Score = 187, P = 1. le-14

PIR:A40533 cAMP-dependent protein kinase major membrane substrate precursor - dog, N = 1, Score = 189, P = 6.5e-15

SWISSPROT :PLM_RAT PHOSPHOLEMMAN PRECURSOR., N = 1, Score = 185, P = 1.7e-14

>SWISSPROT:PLM_HUMAN PHOSPHOLEMMAN PRECURSOR. Length = 92

HSPs:

Score = 196 (29.4 bits), Expect = 1.2e-15, P = 1.2e-15 Identities = 43/85 (50%), Positives = 56/85 (65%)

Query: 4 VLVFLCSLLAPMVLASAAEKEKEMDPFHYDYQTLRIGGLVFAVVLFSVGILLILSRRCKC 63

+LVF LL + AE KE DPF YDYQ+L+IGGLV A +LF +GIL++LSRRC+C Sbjct: 7 ILVFCVGLLT MAKAESPKEHDPFTYDYQSLQIGGLVIAGILFILGILIVLSRRCRC 62

Query: 64 SFNQKPRA--PGDEEAQVENLITANAT 88

FNQ+ R P +EE + 1 +T Sbjct: 63 KFNQQQRTGEPDEEEGTFRSSIRRLST 89

Pedant information for DKFZphfbr2_82ιl7, frame 2

Report for DKFZphfbr2_82ιl7.2

[LENGTH] 95

[MW] 10542.37

[pi] 5.05

[HOMOL] SWISSPROT: PLM_HUMAN PHOSPHOLEMMAN PRECURSOR. 3e-15

[BLOCKS] BL01310 [EC] 3.6.1.37 Na+/K+-exchangmg ATPase 6e-08

[PIRKW] transmembrane protein le-09

[PIRKW] hydrolase 6e-08

[PROSITE] ATP1G1_PLM_MAT8 1

[PROSITE] MYRISTYL 1

[PROSITE] CK2_PHOSPHO_SITE 1

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 2

[PROSITE] ASN_GLYCOSYLATION 1

[KW] Alpha_Beta

[KW] SIGNAL PEPTIDE 19

SEQ MELVLVFLCSLLAPMVLASAAEKEKEMDPFHYDYQTLRIGGLVFAWLFSVGILLILSRR PRD ccchhhhhhhhhhccccccccccccccccccceeeeecccceeeehhhhhhheeeeehhh

SEQ CKCSFNQKPRAPGDEEAQVENLITANATEPQKAEN

PRD hhhcccccccccccchhhhhhhhhhhccccccccc

Prosite for DKFZphfbr2_82ιl7.2

PS00001 86->90 ASN_GLYCOSYLATION PDOC00001 PS00005 36->39 PKC_PHOSPHO_SITE PDOC00005 PS00005 58->61 PKC_PHOSPHO_SITE PDOC00005 PS00006 19->23 CK2_PHOSPHO_SITE PDOC00006 PS00007 25->33 TYR_PHOSPHO_SITE PDOC00007 PS00008 41->47 MYRISTYL PDOC00008 PS01310 28->42 ATP1G1 PLM MAT8 PDOC01014

(No Pfam data available for DKFZphfbr2_82ιl7.2)

DKFZphfbr2_82ι24

group: nucleic acid management

DKFZphfbr2_82ι24 encodes a novel 547 ammo acid protein with similarity to DEAD-box superfamily ATP-dependent helicases.

RNA helicases comprise a large family of proteins that are involved in basic biological systems such as nuclear and mitochondrial splicing processes, RNA editing, rRNA processing, translation initiation, nuclear mRNA export, and mRNA degradation. RNA helicases are essential factors m cell development and differentiation, and some of them play a role in transcription and replication of viral single-stranded RNA genomes. The members of the largest subgroup, the DEAD and DEAH box proteins, exhibit a strong dependence of the unwinding activity on ATP hydrolysis .

The novel protein contains a DEAD-box an ATP/GTP-b dmg site motif A (P-loop, interacting with one of the phophate groups of the nucleotide) and a leucine zipper. Mutations in the closely related Drosophila Hlc gene result in lethality in homozygotes. Therefore the new protein seems to be critical involved in RNA processing in eukaπontic c ells.

The new protein can find application in modulating RNA metabolism and gene expression. strong similarity to DEAD-box subfamily ATP-dependent helicase complete cDNA, complete eds, EST hits potential Start at Bp 9 matches Kozak consensus PyNNatgG,

[PFAM] Helicases conserved C-terminal domain

[PFAM] DEAD and DEAH box helicases

Sequenced by DKFZ

Locus: /map="720_A_3; 758_H_4; 772_E_3; 804_A_5; 175.5 cR from topFT of Chr7 linkage group"

Insert length: 1860 bp

Poly A stretch at pos. 1850, polyadenylation signal at pos. 1829

1 AGCAGCGCCA TGGAGGACTC TGAAGCACTG GGCTTCGAAC ACATGGGCCT

51 CGATCCCCGG CTCCTTCAGG CTGTCACCGA TCTGGGCTGG TCGCGACCTA

101 CGCTGATCCA GGAGAAGGCC ATCCCACTGG CCCTAGAAGG GAAGGACCTC

151 CTGGCTCGGG CCCGCACGGG CTCCGGGAAG ACGGCCGCTT ATGCTATTCC

201 GATGCTGCAG CTGTTGCTCC ATAGGAAGGC GACAGGTCCG GTGGTAGAAC

251 AGGCAGTGAG AGGCCTTGTT CTTGTTCCTA CCAAGGAGCT GGCACGGCAA

301 GCACAGTCCA TGATTCAGCA GCTGGCTACC TACTGTGCTC GGGATGTCCG

351 AGTGGCCAAT GTCTCAGCTG CTGAAGACTC AGTCTCTCAG AGAGCTGTGC

401 TGATGGAGAA GCCAGATGTG GTAGTAGGGA CCCCATCTCG CATATTAAGC

451 CACTTGCAGC AAGACAGCCT GAAACTTCGT GACTCCCTGG AGCTTTTGGT

501 GGTGGACGAA GCTGACCTTC TTTTTTCCTT TGGCTTTGAA GAAGAGCTCA

551 AGAGTCTCCT CTGTCACTTG CCCCGGATTT ACCAGGCTTT TCTCATGTCA

601 GCTACTTTTA ACGAGGACGT ACAAGCACTC AAGGAGCTGA TATTACATAA

651 CCCGGTTACC CTTAAGTTAC AGGAGTCCCA GCTGCCTGGG CCAGACCAGT

701 TACAGCAGTT TCAGGTGGTC TGTGAGACTG AGGAAGACAA ATTCCTCCTG

751 CTGTATGCCC TGCTCAAGCT GTCATTGATT CGGGGCAAGT CTCTGCTCTT

801 TGTCAACACT CTAGAACGGA GTTACCGGCT ACGCCTGTTC TTGGAACAGT

851 TCAGCATCCC CACCTGTGTG CTCAATGGAG AGCTTCCACT GCGCTCCAGG

901 TGCCACATCA TCTCACAGTT CAACCAAGGC TTCTACGACT GTGTCATAGC

951 AACTGATGCT GAAGTCCTGG GGGCCCCAGT CAAGGGCAAG CGTCGGGGCC

1001 GAGGGCCCAA AGGGGACAAG GCCTCTGATC CGGAAGCAGG TGTGGCCCGG

1051 GGCATAGACT TCCACCATGT GTCTGCTGTG CTCAACTTTG ATCTTCCCCC

1101 AACCCCTGAG GCCTACATCC ATCGAGCTGG CAGGACAGCA CGCGCTAACA

1151 ACCCAGGCAT AGTCTTAACC TTTGTGCTTC CCACGGAGCA GTTCCACTTA

1201 GGCAAGATTG AGGAGCTTCT CAGTGGAGAG AACAGGGGCC CCATTCTGCT

1251 CCCCTACCAG TTCCGGATGG AGGAGATCGA GGGCTTCCGC TATCGCTGCA

1301 GGGATGCCAT GCGCTCAGTG ACTAAGCAGG CCATTCGGGA GGCAAGATTG

1351 AAGGAGATCA AGGAAGAGCT TCTGCATTCT GAGAAGCTTA AGACATACTT

1401 TGAAGACAAC CCTAGGGACC TCCAGCTGCT GCGGCATGAC CTACCTTTGC

1451 ACCCCGCAGT GGTGAAGCCC CACCTGGGCC ATGTTCCTGA CTACCTGGTT

1501 CCTCCTGCTC TCCGTGGCCT GGTACGCCCT CACAAGAAGC GGAAGAAGCT

1551 GTCTTCCTCT TGTAGGAAGG CCAAGAGAGC AAAGTCCCAG AACCCACTGC

1601 GCAGCTTCAA GCACAAAGGA AAGAAATTCA GACCCACAGC CAAGCCCTCC

1651 TGAGGTTGTT GGGCCTCTCT GGAGCTGAGC ACATTGTGGA GCACAGGCTT

1701 ACACCCTTCG TGGACAGGCG AGGCTCTGGT GCTTACTGCA CAGCCTGAAC

1751 AGACAGTTCT GGGGCCGGCA GTGCTGGGCC CTTTAGCTCC TTGGCACTTC

1801 CAAGCTGGCA TCTTGCCCCT TGACAACAGA ATAAAAATTT TAGCTGCCCC

1851 AAAAAAAAAA

BLAST Results Entry HSG05793 from database EMBL: human STS WI-6581.

Length = 206

Minus Strand HSPs:

Score = 992 (148.8 bits), Expect = 6.0e-38, P = 6.0e-38

Identities = 204/208 (98%), Positives = 204/208 (98%), Strand = Minus /

PI

Entry AC004938 from database EMBL:

Homo sapiens clone DJ0971C03; HTGS phase 1, 18 unordered pieces.

Score = 1269, P = 6.5e-202, identities = 269/282

12 exons Bp -87920-93706 (matching 1-1497)

Medline entries

No Medlme entry

Peptide information for frame 1

ORF from 10 bp to 1650 bp; peptide length: 547 Category: strong similarity to known protein Classification: Nucleic acid management Prosite motifs: ATP_GTP_A (51-59) LEUCINE ZIPPER (149-171)

1 MEDSEALGFE HMGLDPRLLQ AVTDLGWSRP TLIQEKAIPL ALEGKDLLAR

51 ARTGSGKTAA YAIPMLQLLL HRKATGPVVE QAVRGLVLVP TKELARQAQS

101 MIQQLATYCA RDVRVANVSA AEDSVSQRAV LMEKPDVVVG TPSRILSHLQ

151 QDSLKLRDSL ELLVVDEADL LFSFGFEEEL KSLLCHLPRI YQAFLMSATF

201 NEDVQALKEL ILHNPVTLKL QESQLPGPDQ LQQFQVVCET EEDKFLLLYA

251 LLKLSLIRGK SLLFVNTLER SYRLRLFLEQ FSIPTCVLNG ELPLRSRCHI

301 ISQFNQGFYD CVIATDAEVL GAPVKGKRRG RGPKGDKASD PEAGVARGID

351 FHHVSAVLNF DLPPTPEAYI HRAGRTARAN NPGIVLTFVL PTEQFHLGKI

401 EELLSGENRG PILLPYQFRM EEIEGFRYRC RDAMRSVTKQ AIREARLKEI

451 KEELLHSEKL KTYFEDNPRD LQLLRHDLPL HPAVVKPHLG HVPDYLVPPA

501 LRGLVRPHKK RKKLSSSCRK AKRAKSQNPL RSFKHKGKKF RPTAKPS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfbr2_82ι24, frame 1

TREMBL:AF017777_10 gene: "hlc"; product: "helicase"; Drosophila melanogaster tweety (tty) , flightless (fli), dodo (dod) , penguin (pen), small optic lobes (sol), innocent bystander (iby), waclaw (waw), bobby sox (bbx) , sluggish (slg) , helicase (hlc) , misato (mst) , and la costa (lcs) genes, complete eds., N = 1, Score = 1230, P = 3.2e-125

TREMBL:SPCC1494_6 gene: "SPCC1494.06c"; product: "atp dependent helicase"; S. pombe chromosome II cosmid C1494., N = 2, Score = 753, P = 2.5e-113

PIR:S51412 hypothetical protein YLR276c - yeast (Saccharomyces cerevisiae), N = 2, Score = 711, P = 8.2e-117

TREMBL :AF025451_2 gene: "C24H12.4"; Caenorhabditis elegans cosmid C24H12., N = 2, Score = 564, P = 2.7e-99

>TREMBL:AF017777_10 gene: "hlc"; product: "helicase"; Drosophila melanogaster tweety (tty), flightless (fli), dodo (dod), penguin (pen), small optic lobes (sol), innocent bystander (iby), waclaw (waw), bobby sox (bbx), sluggish (slg), helicase (hlc), misato (mst), and la costa (lcs) genes, complete eds. Length = 560

HSPs:

Score = 1230 (184.5 bits), Expect = 3.2e-125, P = 3.2e-125 Identities = 251/497 (50%), Positives = 344/497 (69%) Query: 9 FEHMGLDPRLLQAVTDLGWSRPTLIQEKAIPLALEGKDLLARARTGSGKTAAYAIPMLQL 68

F + LD R+L+AV LGW +PTLIQ AIPL LEGKD++ RARTGSGKTA YA+P++Q Sb_jct: 11 FHELELDQRILKAVAQLGWQQPTLIQSTAIPLLLEGKDVVVRARTGSGKTATYALPLIQK 70

Query: 69 LLHRKATGPVVEQAVRGLVLVPTKELARQAQSMIQQLATYCARDVRVANVS-AAEDΞVSQ 127

+L+ K EQ V +VL PTKEL RQ++ +I+QL C + VRVA+++ ++ D+V+Q Sbjct: 71 ILNSKLNAS—EQYVSAVVLAPTKELCRQSRKVIEQLVESCGK VRVADIADSSNDTVTQ 128

Query: 128 RAVLMEKPDVVVGTPSRILSHLQQDSLKLRDSLELLVVDEADLLFSFGFEEELKSLLCHL 187

R L E PD+VV TP+ +L++ + S+ +E LVVDEADL+F++G+E++ K L+ HL Sbjct: 129 RHALSESPDIVVATPANLLAYAEAGSVVDLKHVETLVVDEADLVFAYGYEKDFKRLIKHL 188

Query: 188 PRIYQAFLMSATFNEDVQALKELILHNPVTLKLQESQLPGPDQLQQFQVVCETEEDKFLL 247

P IYQA L+SAT +DV +K L L+NPVTLKL+E +L DQL +++ E E DK + Sbjct: 189 PPIYQAVLVSATLTDDVVRMKGLCLNNPVTLKLEEPELVPQDQLSHQRILAE-ENDKPAI 247

Query: 248 LYALLKLSLIRGKSLLFVNTLERSYRLRLFLEQFSIPTCVLNGELPLRSRCHIISQFNQG 307

LYALLKL LIRGKS++FVN+++R Y++RLFLEQF I CVLN ELP R H ISQFN+G Sbjct: 248 LYALLKLRLIRGKSIIFVNSIDRCYKVRLFLEQFGIRACVLNSELPANIRIHTISQFNKG 307

Query: 308 FYDCVIATDAEVLGAPVKGKRRGRGPKGDKASDPEAGVARGIDFHHVSAVLNFDLPPTPE 367

YD +IA+D + P G + K ++ D E+ +RGIDF V+ V+NFD P Sbjct: 308 TYDIIIASDEHHMEKP—GGKSATNRKSPRSGDMESSASRGIDFQCVNNVINFDFPRDVT 365

Query: 368 AYIHRAGRTARANNPGIVLTFVLPTEQFHLGKIEELL SGENRGPILLPYQFRMEEI 423

+YIHRAGRTAR NN G VL+FV E +E+ L + + 1+ YQF+MEE+ Sbjct: 366 SYIHRAGRTARGNNKGSVLΞFVSMKESKVNDSVEKKLCDSFAAQEGEQIIKNYQFKMEEV 425

Query: 424 EGFRYRCRDAMRSVTKQAIREARLKEIKEELLHSEKLKTYFEDNPRDLQLLRHDLPLHPA 483

E FRYR +D R+ T+ A+ + R++EIK E+L+ EKLK +FE+N RDLQ LRHD PL Sbjct: 426 ESFRYRAQDCWRAATRVAVHDTRIREIKIEILNCEKLKAFFEENKRDLQALRHDKPLRAI 485

Query: 484 VVKPHLGHVPDYLVPPALRGLV 505

V+ HL +P+Y+VP AL+ +V Sbjct: 486 KVQSHLSDMPEYIVPKALKRVV 507

Pedant information for DKFZphfbr2_82ι24, frame 1

Report for DKFZphfbr2_82ι24.1

[LENGTH] 547

[MW] 61589.88

[pi] 9.34

[HOMOL] TREMBL:AF017777_10 gene: "hlc"; product: "helicase"; Drosophila melanogaster tweety (tty) flightless (fli), dodo (dod), penguin (pen), small optic lobes (sol), innocent bystander (ι:by), waclaw (waw), bobby sox (bbx), sluggish (slg), helicase (hlc), misato (mst), and la costa (lcs) genes, complete eds. le-121

[FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YLR276c] le-109

[FUNCAT] mrna translation and ribosome biogenesis [H. influenzae, HI0231 RNA]

2e-42

[FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YLL008w] 8e-40

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YLL008w] 8e-40

[FUNCAT) 30.10 nuclear organization [S. cerevisiae, YLL008w] 8e-40

[FUNCAT] 05.04 translation (initiation, elongation and termination) [ S . cerevisiae YKR059w] 3e-39

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YKR059w ] 3e-39

[FUNCAT] 04.99 other transcription activities [S. cerevisiae, YDL160c ] 3e-35

[FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YPL119c ] 3e-29

[FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YMR290c ] 4e-29

[FUNCAT] 1 genome replication, transcription, recombination and repair [H. influenzae HI0892] le-27

[FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YJL033w] 2e-27

[FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YDR194c] 4e-21

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YGL064C] le-05

[BLOCKS] BL00039D DEAD-box subfamily ATP-dependent helicases proteins

[BLOCKS] BL00039C DEAD-box subfamily ATP-dependent helicases proteins

[BLOCKS] BL00039B DEAD-box subfamily ATP-dependent helicases proteins

[BLOCKS] BL00039A DEAD-box subfamily ATP-dependent helicases proteins

[PIRKW] nucleus 4e-34

[PIRKW] RNA binding 7e-41

[PIRKW] DEAD box 2e-38

[PIRKW] transmembrane protein 9e-20

[PIRKW] DNA binding 8e-23

[PIRKW] ATP le-107

[PIRKW] purine nucleotide binding 2e-38

[PIRKW] P-loop le-107

[PIRKW] hydrolase 2e-35

[PIRKW] protein biosynthesis 2e-38

[PIRKW] ATP binding 7e-43 [SUPFAM] WW repeat homology le-26

[SUPFAM] DEAD/H box helicase homology le-107

[SUPFAM] unassigned DEAD/H box helicases le-107

[SUPFAM] ATP-dependent RNA helicase DBP1 3e-31

[SUPFAM] ATP-dependent RNA helicase DHH1 2e-35

[SUPFAM] translation initiation factor eIF-4A 2e-38

[SUPFAM] tobacco ATP-dependent RNA helicase DB10 le-26

[PROSITE] ATP_GTP_A 1

[PROSITE] LEUCINE_ZIPPER 1

[PFAM] Helicases conserved C-termmal domain

[PFAM] DEAD and DEAH box helicases

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 9.87 %

SEQ MEDSEALGFEHMGLDPRLLQAVTDLGWSRPTLIQEKAIPLALEGKDLLARARTGSGKTAA SEG PRD ccccccccccccccchhhhhhhhhhccccccccccccccccccccceeeeecccccccee

SEQ YAIPMLQLLLHRKATGPVVEQAVRGLVLVPTKELARQAQSMIQQLATYCARDVRVANVSA SEG PRD ehhhhhhhhhhhcccccccccceeeeeeccchhhhhhhhhhhhhhhhhhhcceeeeeecc

SEQ AEDSVSQRAVLMEKPDVVVGTPSRILSHLQQDSLKLRDSLELLVVDEADLLFSFGFEEEL

SEG xxxxxxxxxxxx

PRD ccchhhhhhhhhcccceeeeccccchhhhhhcccccchhhhhhhhhhhhhhhhhcchhhh

SEQ KSLLCHLPRIYQAFLMSATFNEDVQALKELILHNPVTLKLQESQLPGPDQLQQFQVVCET SEG PRD hhhhhhccchhhhhhhhhccchhhhhhhhhhhcccceeeeeccccccchhhhhhhhhhhh

SEQ EEDKFLLLYALLKLSLIRGKSLLFVNTLERSYRLRLFLEQFSIPTCVLNGELPLRSRCHI

SEG xxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhccceeeeeeehhhhhhhhhhhhhhcccceeeccccchhhhhhhh

SEQ ISQFNQGFYDCVIATDAEVLGAPVKGKRRGRGPKGDKASDPEAGVARGIDFHHVSAVLNF

SEG xxxxxxxxxxxxx

PRD hhhhhccceeeeeeccccccccccccccccccccccccccccccccccccccceeeeeec

SEQ DLPPTPEAYIHRAGRTARANNPGIVLTFVLPTEQFHLGKIEELLSGENRGPILLPYQFRM SEG PRD ccccccceeeeccccccccccccceeeeeecchhhhhhhhhhhhhhhccccccccccchh

SEQ EEIEGFRYRCRDAMRSVTKQAIREARLKEIKEELLHSEKLKTYFEDNPRDLQLLRHDLPL

SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccchhhhhhhhccc

SEQ HPAVVKPHLGHVPDYLVPPALRGLVRPHKKRKKLSSSCRKAKRAKSQNPLRSFKHKGKKF SEG xxxxxxxxxxxxxxxxxx

PRD cccccccccccccceeeccccccccccccccccccchhhhhhcccccccccccccccccc

SEQ RPTAKPS SEG PRD ccccccc

Prosite for DKFZphfbr2_82ι24.1

PS00017 51->59 ATP_GTP_A PDOC00017 PS00029 149->171 LEUCINE ZIPPER PDOC00029

Pfam for DKFZphfbr2_82ι24.1

HMM_NAME DEAD and DEAH box helicases

HMM *gLpPWILRnIyeMGFEkPTPIQQqAIPιILeGRDVMACAQTGSGKTAAF GL+P +L +++++G+++PT IQ++AIP++LEG+D++A+A TGSGKTAA+

Query 13 GLDPRLLQAVTDLGWSRPTLIQEKAIPLALEGKDLLARARTGSGKTAAY 61

HMM HPMLQHIDwdP... WpqpPQdPrALILAPTRELAMQIQEEcRkFgkHMn +IPMLQ +++ + + + +R+L+L+PT ELA+Q Q +++++ ++

Query 62 AIPMLQLLLHRKATGPVVEQA-VRGLVLVPTKELARQAQSMIQQLATYCA 110

HMM g.IRImcIYGGtnMRdQMRmLeRGpPHIVIATPGRLIDHIERgtldLDr. +R++ + + Q +L+++P ++V++TP R++ H+++ +L+L++

Query 111 RDVRVANVSAAEDSVSQRAVLMEKP-DVVVGTPSRILSHLQQDSLKLRDS 159

HMM IeMLVMDEADRMLDMGFIDQIRrlMrqlPMpwNRQTMMFSATMPdelqEL +E LV DEAD +++ GF++++ ++ ++P + Q + SAT+ +++Q L Query 160 LELLVVDEADLLFSFGFEEELKSLLCHLP—RIYQAFLMSATFNEDVQAL 207

HMM ARrFMRNPIRInldMdElTtnEnlkQwYiyVerEMWKfdcLcrLIe*

+ +++NP+ + + +++L + ++Q+ +++E E++KF +L+ L++ Query 208 KELILHNPVTLKLQESQLPGPDQLQQFQVVCETEEDKFLLLYALLK 253

HMM_NAME Helicases conserved C-terminal domain

HMM *EιleeWLknlGIrvmYIHGdMpQeERdeIMddFNnGEynVLIcTDV...

+L+ +L++ I+++++ G +P + R 1+ +FN+G Y++ I+TD+ Query 272 YRLRLFLEQFSIPTCVLNGELPLRSRCHIISQFNQGFYDCVIATDAEVL 320

HMM ggRGIDIPdVNHVINYDMPWNPEqYI

+RGID+ V+ V N+D+P +PE YI Query 321 GAPVKGKRRGRGPKGDKASDPEAGVARGIDFHHVSAVLNFDLPPTPEAYI 370

HMM QRIGRTgRIG*

+R+GRT+R++ Query 371 HRAGRTARAN 380

DKFZphfbr2_82ml6

group: brain derived

DKFZphfbr2_82ml6 encodes a novel 289 amino acid protein with very weak similarity to A. haliana F28A23.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of brain-specific genes .

similarity to A. thaliana F28A23.140 complete cDNA, complete eds, few EST hits many ATGs in front of the ORF TRANSMEMBRANE 1

Sequenced by DKFZ

Locus: /map="4"

Insert length: 2715 bp

Poly A stretch at pos. 2705, polyadenylation signal at pos. 2687

1 AGAGGAGGGG AGAGGACTGG GGAGCCGAGC CAGAGCCGGG CTGCCTGCCA

51 CCCGGCTGCT CGTCCGCTAG CTGGGGAGGA GCGCTCCACC CGCAACTGAC

101 AAAGGATGGG AGAATGCCCG CGCCCCGGGA TGCCGGCCGC ACGCAGCCTG

151 GCGGCCGCCT GAGCTACTTC ACCCTCCGCC GGTAAGTGAC TGCAAACATC

201 ATTCATTCAA TCAGCCTCAC TGGGAGCCCC TTCTCTCCGG CTGGTAGTCC

251 TGGGCGGCTT GTCCCTGATC CCGAGCGGGG CTTGGCACAG CATCAGCCCT

301 GGAGGGCAGG CAGCAGGTGC CTTTGCCTGG TGGGTCCACT GGGGAGCGTG

351 GCTGGGGTTC GCGGCGGGTG CTGCCACCCA ACCTGCGGGC GGCGGGCTCG

401 CCCAGTAGGC GCCTCTCTGG TGAGAGGAGG CGGCTCCAGC CCGCATCCTG

451 GGGTAGTTGC TACTATTGGC CCCCAGCGCC CGCTCTGCGC GCGCGCCGTT

501 TCTGGCGGAT CCCCAGTGCG CGGCGCGCTG TTTACACCGG CGTGGTACTA

551 GTCACGGAGC CGCACCCCTC GGAAAGCGCG GAGTCGATGA CAGCCACTTC

601 ACAGGCTCAC GCGCTCCTAG TGTGGGCTTG AAGGGGACGG GGACCGATTA

651 CCAAAGGAGA GCGCTGAGTA CGGAAGACAC AGGGCAGCCT TTGTCTTGGG

701 TTTAGCGCTG ATGCGCTCAA CCCTGAGTCG GGTTCACTGC AACTGTTGTG

751 TCCGATTTCG GTTCCCTGCA ACCGCCCTCC TGGGCGAGAG ATGTCATTGT

801 GTTCCTGCGG CCAGCGGGAC TGAGAGCTGG GACTTAAGAC GCCAGGAGGG

851 TCCTGCGCTC ACGGGAAATG TACCCCAAAA GAACTCTGAG AGAATATACT

901 CAACTGTCCT GCTGTGATTA AACAAGACTG CTGTATTTTA ATTTCAGAAA

951 TTGAAAAGGG ATAGGAGGAA GGGGAAAATG CTGGGCTGGT GTGAAGCGAT

1001 AGCCCGTAAC CCTCACAGAA TTCCAAACAA CACGCGAACA CCCGAGATCT

1051 CAGGGGATTT GGCTGACGCC TCACAAACCT CCACATTGAA TGAAAAATCC

1101 CCAGGGCGAT CTGCAAGTCG ATCAAGTAAC ATTTCAAAAG CAAGCAGCCC

1151 AACAACAGGG ACAGCTCCCA GGAGCCAGTC AAGGTTGTCT GTCTGTCCAT

1201 CCACTCAGGA CATCTGCAGA ATCTGTCACT GCGAAGGGGA TGAAGAGAGC

1251 CCCCTCATCA CACCCTGTCG CTGCACTGGG ACACTGCGCT TTGTCCACCA

1301 GTCCTGCCTC CACCAGTGGA TAAAGAGCTC AGATACACGC TGCTGTGAGC

1351 TCTGCAAGTA TGACTTCATA ATGGAGACCA AGCTCAAACC CCTCCGGAAG

1401 TGGGAGAAAC TACAGATGAC CACAAGTGAA AGGAGGAAAA TATTCTGCTC

1451 TGTCACATTC CACGTAATCG CGATCACCTG TGTGGTTTGG TCTTTGTATG

1501 TATTGATAGA CCGGACAGCG GAGGAAATCA AGCAAGGCAA TGACAATGGT

1551 GTCCTTGAAT GGCCATTTTG GACAAAACTG GTTGTGGTAG CCATTGGCTT

1601 CACAGGAGGT CTTGTCTTCA TGTACGTACA GTGTAAAGTC TATGTTCAGT

1651 TGTGGCGCAG GCTGAAGGCC TACAACCGTG TGATCTTTGT ACAAAATTGC

1701 CCAGACACTG CCAAAAAACT GGAGAAGAAC TTCTCATGTA ATGTAAACAC

1751 AGACATCAAA GATGCTGTGG TAGTGCCTGT ACCACAAACA GGTGCAAATT

1801 CACTGCCATC TGCAGAGGGT GGCCCCCCTG AAGTTGTATC AGTCTGATGG

1851 AACCTGTTGG GAGTTTCTTC ACCGAAGAAT ATCTTTCTAG CCCTCAGCCA

1901 CTACAAATGA CAGAAGTGAC CTTGAATTAT TTACTCCCTT CAGCTCCTCC

1951 TTTCTCCTAC TGACACATTT TTCCTGACTT TGTTCAAAGA GGAAAGGAGA

2001 AAAACAAACA AACAGACCAA ATGCCCAGGA GCCCATGAAG TAATAGCGTA

2051 AAGTAAAGTA TGATATGGAA ATGTGAAGTT TGCAAGAGAA TGATTTCCAA

2101 GACAATTAAG AACTACTGGG GCAATGAATG CTTTTAGGCA GTAATCAAAG

2151 ATTAAATGGA CCCATGATAC TCTTCTTCAC AGTAACAGGG GAAAAGTTCA

2201 AGAATACAGA CTTGAATTGC GATGTGTATT ACTTCTAGGG CCTTGTAATG

2251 TTAACTGTCT CATCTGGAAA TAATAACTAA CATATTTGGT TTTAAGCCTG

2301 AAATTGTCTG CATTATCCCT AAGTCACATT GGAAGTGAAC TTGGAGGATG

2351 CATATTTTGA TATGCTTTGA CAGCTAACAG ATTTGTATGG TTTAGTGGAG

2401 TCTGGTTATT TTGACAGATG CATGTTTTTT TTAAATAGAT GCAATATACA

2451 TTTGAAGACA TTGATATTTG GAATTAATTA TGTTTGTTTA AGTCACGCAA

2501 AAGATTTTCA GAAAATGTTC GGATATAATT AGCTCTGTTA AATACCCACA

2551 GAACTGTTAT CAGGTCTTAT ATTTATTTTC ATCTGGTTCC TCTAATACAG 2601 TGCTGTCCAA TAGAAACACA ACAGCCACAA ATGCAGGCCA CAGATGCAAA 2651 TATTTAACTT CCCAGTAGCC CTATTTTAAA AAGTAAAAAT AAATGTTTGT 2701 TTGTTAAAAA AAAAA

BLAST Results

Entry G37457 from database EMBLNEW:

SHGC-57357 Human Homo sapiens STS genomic.

Length = 458

Plus Strand HSPs:

Score = 2116 (317.5 bits), Expect = 4.3e-91, P = 4.3e-91

Identities = 444/456 (97%)

Medline entries

No Medline entry

Peptide information for frame 3

1 MLGWCEAIAR NPHRIPNNTR TPEISGDLAD ASQTSTLNEK SPGRSAΞRSS 51 NISKASSPTT GTAPRSQSRL SVCPSTQDIC RICHCEGDEE SPLITPCRCT 101 GTLRFVHQSC LHQWIKSSDT RCCELCKYDF IMETKLKPLR KWEKLQMTTS 151 ERRKIFCSVT FHVIAITCVV WSLYVLIDRT AEEIKQGNDN GVLEWPFWTK 201 LVVVAIGFTG GLVFMYVQCK VYVQLWRRLK AYNRVIFVQN CPDTAKKLEK 251 NFSCNVNTDI KDAVVVPVPQ TGANSLPSAE GGPPEVVSV

ORF from 978 bp to 1844 bp; peptide length: 289 Category: similarity to unknown protein

BLASTP hits

Entry AB011169_1 from database TREMBL: gene: "KIAA0597"; product: "KIAA0597 protein"; Homo sapiens mRNA for

KIAA0597 protein, partial eds.

Score = 188, P = 6.0e-12, identities = 30/54, positives = 38/54

Entry SPBC14F5_7 from database TREMBL: gene: "SPBC14F5.07"; product: "hypothetical protein"; S. pombe chromosome II cosmid cl4F5.

Score = 185, P = 1.9e-ll, identities = 29/53, positives = 38/53

Entry CEY57A10B_1 from database TREMBL: gene: "Y57A10B.1"; Caenorhabditis elegans cosmid Y57A10B

Score = 171, P = 2.6e-10, identities = 40/107, positives = 58/107

Alert BLASTP hits for DKFZphfbr2_82ml6, frame 3

TREMBL :ATF28A23_14 gene: "F28A23.140"; product: "putative protein"; Arabidopsis thaliana DNA chromosome 4, BAC clone F28A23 (ESSAII project), N = 1, Score = 198, P = 3.4e-13

>TREMBL:ATF28A23_14 gene: "F28A23.140"; product: "putative protein";

Arabidopsis thaliana DNA chromosome 4, BAC clone F28A23 (ESSAII project) Length = 1,051

HSPs:

Score = 198 (29.7 bits), Expect = 3.4e-13, P = 3.4e-13 Identities = 38/103 (36%), Positives = 61/103 (59%)

Query: 28 LADASQTSTLNEKSPGRSASRS-SNISKASSPTTGTAPRSQSRLSVCPSTQDICRICHCE 86

+++ S +S+ + SP +++ SN+ A S TG+ +D+CRIC

Sbjct: 20 VSEPSVSSSΞSSSSPNQASPNPFSNMDPAVSTATGSRYVDDDE DEEDVCRICRNP 74

Query: 87 GDEESPLITPCRCTGTLRFVHQΞCLHQWIKSSDTRCCELCKYDF 130

GD ++PL PC C+G+++FVHQ CL QW+ S+ R CE+CK+ F Sbjct: 75 GDADNPLRYPCACSGSIKFVHQDCLLQWLNHSNARQCEVCKHPF 118 Pedant information for DKFZphfbr2_82ml 6 , frame 3

Report for DKFZphfbr2_82ml 6 . 3

[ LENGTH ] 289

[MW] 32308 . 36

[pi ] 8 . 76

[HOMOL] PIR:T00268 hypothetical protein KIAA0597 - human (fragment) 9e-14

[FUNCAT] 04.99 other transcription activities [S. cerevisiae, YIL030c] 4e-09

[PIRKW] transmembrane protein 9e-08

[PROSITE] MYRISTYL 1

[PROSITE] CK2_PHOSPHO_SITE 4

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOΞPHO_SITE 3

[PROSITE] ASN_GLYCOSYLATION 3

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 6.57 %

SEQ MLGWCEAIARNPHRIPNNTRTPEISGDLADASQTSTLNEKSPGRSASRSSNISKASSPTT SEG xxxxxxxxxxxxxxxxxxx .. PRD ccchhhhhhccccccccccccccccchhhhhhhhhccccccccccccccccccccccccc

SEQ GTAPRSQSRLSVCPSTQDICRICHCEGDEESPLITPCRCTGTLRFVHQSCLHQWIKSSDT SEG PRD ccccccccccccccccceeeeeeecccccccccccccccccceeeeehhhhhhhhhcccc

SEQ RCCELCKYDFIMETKLKPLRKWEKLQMTTSERRKIFCSVTFHVIAITCVVWSLYVLIDRT SEG PRD ceeeeeeehhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccc

SEQ AEEIKQGNDNGVLEWPFWTKLVVVAIGFTGGLVFMYVQCKVYVQLWRRLKAYNRVIFVQN SEG PRD ccccccccccceeehhhhheeeeeeecccccceeeeehhhhhhhhhhhhhhhheeeeeee

SEQ CPDTAKKLEKNFSCNVNTDIKDAVVVPVPQTGANSLPSAEGGPPEVVSV SEG PRD ccchhhhhhccccccccccceeeeeeecccccccccccccccccccccc

Prosite for DKFZphfbr2_82ml6.3

PS00001 17->21 ASN_GLYCOSYLATION PDOC00001 PS00001 51->55 ASN_GLYCOSYLATION PDOC00001 PS00001 251->255 ASN_GLYCOSYLATION PDOC00001 PS00005 102-M 05 PKC_PHOSPHO_SITE PDOC00005 PS00005 150->153 PKC_PHOS PHO_S ITE PDOC00005 PS00005 244 ->247 PKC_PHOS PHO_S ITE PDOC00005 PS00006 36->40 CK2_PHOS PHO_S ITE PDOC00006 PS00006 75->79 CK2_PHOSPHO_SITE PDOC00006 PS00006 148 ->152 CK2_PHOSPHO_SITE PDOC00006 PS00006 180-M84 CK2_PHOSPHO_SITE PDOC00006 PS00007 121 -> 129 TYR_PHOSPHO_S ITE PDOC00007 PS00008 187 ->193 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfbr2_82ml6.3)

DKFZphfbr2_82m6

group: signal transduction

DKFZphfbr2_82m6.3 encodes a novel 654 amino acid protein with similarity to murine sphingosine kinase .

Sphingosine kinase is a new type of lipid kinase, which is regulated by growth factors. The enzyme phosphorylates sphingosine, which subsequently exerts intracellular and extracellular actions. Intracellulary, sphingosine 1-phosphate (SPP) promotes proliferation and inhibits apoptosis. In yeast, survival of cells exposed to heat shock indicates is dependend on SPP. Extracellulary, SPP inhibits cell motility and influences cell morphology, effects that appear to be mediated by the G protein-coupled receptor EDG1.

The new protein can find application in modulating/blocking the shingosine kinase intracellular signal transmission pathway. strong similarity to mouse "sphingosine kinase" complete cDNA, complete eds, EST hits,

YLR260w/YOR171c Lcb5p/Lcb4p = long chain base kinases, involved in biosynthesis of sphingol pids

Sequenced by DKFZ

Locus : unknown

Insert length: 2875 bp

Poly A stretch at pos. 2865, polyadenylation signal at pos. 2838

1 AGTGTTGGAG GTGAGGAGGC GGGGCTGGCA GGGCTAGTCG GGGCATCTGG

51 AAATTTCCGA CCCCACGCTT CGGGCGTTTC CTTATCAGGT TCACCGCTCC

101 CTGATCTCGC GCTGCACTTC GTAGGCGCAG CCGCTGCTTG GGAAGTCCTA

151 CTTAAGAGCT GAAGGTCAGG CCAGGACAGT GAGACCTGAC TCCTTGCTCC

201 TACCAGCCTA CTATGGCTTA AGACCCAGGG CCAGGGTCCC GTTGATGTAA

251 CAGAGCAGAG GACCAGCAGA TGAATGGACA CCTTGAAGCA GAGGAGCAGC

301 AGGACCAGAG GCCAGACCAG GAGCTGACCG GGAGCTGGGG CCACGGGCCT

351 AGGAGCACCC TGGTCAGGGC TAAGGCCATG GCCCCGCCCC CACCGCCACT

401 GGCTGCCAGC ACCTCGCTCC TCCATGGCGA GTTTGGCTCC TACCCAGCCC

451 GAGGCCCACG CTTTGCCCTC ACCCTTACAT CGCAGGCCCT GCACATACAG

501 CGGCTGCGCC CCAAACCTGA AGCCAGGCCC CGGGGTGGCC TGGTCCCGTT

551 GGCCGAGGTC TCAGGCTGCT GCACCCTGCG AAGCCGCAGC CCCTCAGACT

601 CAGCGGCCTA CTTCTGCATC TACACCTACC CTCGGGGCCG GCGCGGGGCC

651 CGGCGCAGAG CCACTCGCAC CTTCCGGGCA GATGGGGCCG CCACCTACGA

701 AGAGAACCGT GCCGAGGCCC AGCGCTGGGC CACTGCCCTC ACCTGTCTGC

751 TCCGAGGACT GCCACTGCCC GGGGATGGGG AGATCACCCC TGACCTGCTA

801 CCTCGGCCGC CCCGGTTGCT TCTATTGGTC AATCCCTTTG GGGGTCGGGG

851 CCTGGCCTGG CAGTGGTGTA AGAACCACGT GCTTCCCATG ATCTCTGAAG

901 CTGGGCTGTC CTTCAACCTC ATCCAGACAG AACGACAGAA CCACGCCCGG

951 GAGCTGGTCC AGGGGCTGAG CCTGAGTGAG TGGGATGGCA TCGTCACGGT

1001 CTCGGGAGAC GGGCTGCTCC ATGAGGTGCT GAACGGGCTC CTAGATCGCC

1051 CTGACTGGGA GGAAGCTGTG AAGATGCCTG TGGGCATCCT CCCCTGCGGC

1101 TCGGGCAACG CGCTGGCCGG AGCAGTGAAC CAGCACGGGG GATTTGAGCC

1151 AGCCCTGGGC CTCGACCTGT TGCTCAACTG CTCACTGTTG CTGTGCCGGG

1201 GTGGTGGCCA CCCACTGGAC CTGCTCTCCG TGACGCTGGC CTCGGGCTCC

1251 CGCTGTTTCT CCTTCCTGTC TGTGGCCTGG GGCTTCGTGT CAGATGTGGA

1301 TATCCAGAGC GAGCGCTTCA GGGCCTTGGG CAGTGCCCGC TTCACACTGG

1351 GCACGGTGCT GGGCCTCGCC ACACTGCACA CCTACCGCGG ACGCCTCTCC

1401 TACCTCCCCG CCACTGTGGA ACCTGCCTCG CCCACCCCTG CCCATAGCCT

1451 GCCTCGTGCC AAGTCGGAGC TGACCCTAAC CCCAGACCCA GCCCCGCCCA

1501 TGGCCCACTC ACCCCTGCAT CGTTCTGTGT CTGACCTGCC TCTTCCCCTG

1551 CCCCAGCCTG CCCTGGCCTC TCCTGGCTCG CCAGAACCCC TGCCCATCCT

1601 GTCCCTCAAC GGTGGGGGCC CAGAGCTGGC TGGGGACTGG GGTGGGGCTG

1651 GGGATGCTCC GCTGTCCCCG GACCCACTGC TGTCTTCACC TCCTGGCTCT

1701 CCCAAGGCAG CTCTACACTC ACCCGTCTCC GAAGGGGCCC CCGTAATTCC

1751 CCCATCCTCT GGGCTCCCAC TTCCCACCCC TGATGCCCGG GTAGGGGCCT

1801 CCACCTGCGG CCCGCCCGAC CACCTGCTGC CTCCGCTAGG CACCCCGCTG

1851 CCCCCAGACT GGGTGACGCT GGAGGGGGAC TTTGTGCTCA TGTTGGCCAT

1901 CTCGCCCAGC CACCTAGGCG CTGACCTGGT GGCAGCTCCG CATGCGCGCT

1951 TCGACGACGG CCTGGTGCAC CTGTGCTGGG TGCGTAGCGG CATCTCGCGG

2001 GCTGCGCTGC TGCGCCTTTT CTTGGCCATG GAGCGTGGTA GCCACTTCAG

2051 CCTGGGCTGT CCGCAGCTGG GCTACGCCGC GGCCCGTGCC TTCCGCCTAG

2101 AGCCGCTCAC ACCACGCGGC GTGCTCACAG TGGACGGGGA GCAGGTGGAG

2151 TATGGGCCGC TACAGGCACA GATGCACCCT GGCATCGGTA CACTGCTCAC

2201 TGGGCCTCCT GGCTGCCCGG GGCGGGAGCC CTGAAACTAA ACAAGCTTGG

2251 TACCCGCCGG GGGCGGGGCC TACATTCCAA TGGGGCGGAG CCTGAGCTAG

2301 GGGGTGTGGC CTGGCTGCTA GAGTTGTGGT GGCAGGGGCC CTGGCCCCGT 2351 CTCAGGATTG CGCTCGCTTT CATGGGACCA GACGTGATGC TGGAAGGTGG

2401 GCGTCGTCAC GGTTAAAGAG AAATGGGCTC GTCCCGAGGG TAGTGCCTGA

2451 TCAATGAGGG CGGGGCCTGG CGTCTGATCT GGGGCCGCCC TTACGGGGCA

2501 GGGCTCAGTC CTGACGCTTG CCACCTGCTC CTACCCGGCC AGGATGGCTG

2551 AGGGCGGAGT CTATTTTACG CGTCGCCCAA TGACAGGACC TGGAATGTAC

2601 TGGCTGGGGT AGGCCTCAGT GAGTCGGCCG GTCAGGGCCC GCAGCCTCGC

2651 CCCATCCACT CCGGTGCCTC CATTTAGCTG GCCAATCAGC CCAGGAGGGG

2701 CAGGTTCCCC GGGGCCGGCG CTAGGATTTG CACTAATGTT CCTCTCCCCG

2751 CGGGTGGGGG CGGGGAAATT CATATCCCCT GTTCGTCTCA TGCGCGTCCT

2801 CCGTCCCCAA TCTAAAAAGC AATTGAAAAG GTCTATGCAA TAAAGGCAGT

2851 CGCTTCATTC CTCTCAAAAA AAAAA

BLAST Results

No BLAST result

Medline entries

99045661:

Tumor necrosis factor-alpha induces adhesion molecule expression through the sphingosine kinase pathway.

98395082:

Molecular cloning and functional characterization of murine sphingosine kinase.

98241633:

Purification and characterization of rat kidney sphingosine kinase.

99178622:

Sphingosine 1-phosphate: a prototype of a new class of second messengers .

Peptide information for frame 3

1 MNGHLEAEEQ QDQRPDQELT GSWGHGPRST LVRAKAMAPP PPPLAASTSL

51 LHGEFGSYPA RGPRFALTLT SQALHIQRLR PKPEARPRGG LVPLAEVSGC

101 CTLRSRSPSD SAAYFCIYTY PRGRRGARRR ATRTFRADGA ATYEENRAEA

151 QRWATALTCL LRGLPLPGDG EITPDLLPRP PRLLLLVNPF GGRGLAWQWC

201 KNHVLPMISE AGLSFNLIQT ERQNHARELV QGLSLSEWDG IVTVSGDGLL

251 HEVLNGLLDR PDWEEAVKMP VGILPCGSGN ALAGAVNQHG GFEPALGLDL

301 LLNCSLLLCR GGGHPLDLLS VTLASGSRCF SFLSVAWGFV SDVDIQSERF

351 RALGSARFTL GTVLGLATLH TYRGRLSYLP ATVEPASPTP AHSLPRAKSE

401 LTLTPDPAPP MAHSPLHRSV SDLPLPLPQP ALASPGSPEP LPILSLNGGG

451 PELAGDWGGA GDAPLSPDPL LSSPPGSPKA ALHSPVSEGA PVIPPSSGLP

501 LPTPDARVGA STCGPPDHLL PPLGTPLPPD WVTLEGDFVL MLAISPSHLG

551 ADLVAAPHAR FDDGLVHLCW VRSGISRAAL LRLFLAMERG SHFSLGCPQL

601 GYAAARAFRL EPLTPRGVLT VDGEQVEYGP LQAQMHPGIG TLLTGPPGCP

651 GREP

ORF from 270 bp to 2231 bp; peptide length: 654 Category: similarity to known protein

BLASTP hits

Entry SPAC4A8_7 from database TREMBL: gene: "SPAC4A8.07c"; product: "hypothetical protein"; S. pombe chromosome I cosmid c4A8.

Score = 301, P = 7.9e-32, identities = 68/190, positives = 109/190

Entry CEC34C6_3 from database TREMBLNEW: product: "C34C6.5"; Caenorhabditis elegans cosmid C34C6

>TREMBL:CEC34C6_3 product: "C34C6.5"; Caenorhabditis elegans cosmid

C34C6

Score = 273, P = 9.0e-29, identities = 78/265, positives = 142/265

Entry S67059 from database PIR: hypothetical protein YOR171c - yeast (Saccharomyces cerevisiae) >TREMBL:SC55021_9 gene: "03615"; product: "03615p"; Saccharomyces cerevisiae cosmid pUOA1258 from chromosome 15R. >TREMBL: SCYOR170W_2 S. cerevisiae chromosome XV reading frame ORF YOR170w Score = 253, P = 2.0e-25, identities = 70/234, positives = 116/234

Entry S51398 from database PIR: hypothetical protein YLR260w - yeast (Saccharomyces cerevisiae)

>TREMBL:SCL8479_4 gene: "YLR260W"; product: "Ylr260wp"; Saccharomyces cerevisiae chromosome XII cosmid 8479.

Score = 251, P = 1.0e-24, identities = 62/198, positives = 103/198

Alert BLASTP hits for DKFZphfbr2_82m6, frame 3

TREMBL:AF068749_1 gene: "SPHKlb"; product: "sphingosine kinase"; Mus musculus sphingosine kinase (SPHKlb) mRNA, complete eds., N = 2, Score = 615, P = 1.2e-92

TREMBL:AF068748_1 gene: "SPHKla"; product: "sphingosine kinase"; Mus musculus sphingosine kinase (SPHKla) mRNA, partial eds., N = 2, Score = 616, P = 2e-92

TREMBL:ATF18E5_16 gene: "F18E5.160"; product: "putative protein"; Arabidopsis thaliana DNA chromosome 4, BAC clone F18E5 (ESSAII project), N = 2, Score = 370, P = 6.8e-33

>TREMBL:AF068748_1 gene: "SPHKla"; product: "sphingosine kinase"; Mus musculus sphingosine kinase (SPHKla) mRNA, partial eds. Length = 504

HSPs:

Score = 616 (92.4 bits), Expect = 2.0e-92, Sum P(2) = 2.0e-92 Identities = 128/260 (49%), Positives = 173/260 (66%)

Query: 154 ATALTCLLRGLPLPGDGEITPDLLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGL 213

A C L + E LLPRP R+L+L+NP GG+G A Q ++ V P + EA + Sbjct: 110 APVAPCQREPRDLAMEPECPRGLLPRPCRVLVLLNPQGGKGKALQLFQSRVQPFLEEAEI 169

Query: 214 SFNLIQTERQNHARELVQGLSLSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGI 273

+F LI TER+NHARELV L WD + +SGDGL+HEV+NGL++RPDWE A++ P+ Sbjct: 170 TFKLILTERKNHARELVCAEELGHWDALAVMSGDGLMHEVVNGLMERPDWETAIQKPLCS 229

Query: 274 LPCGSGNALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFL 333

LP GSGNALA +VN + G+E DLL+NC+LLLCR P++LLS+ ASG R +Ξ L Sbjct: 230 LPGGSGNALAASVNHYAGYEQVTNEDLLINCTLLLCRRRLSPMNLLSLHTASGLRLYSVL 289

Query: 334 SVAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPA-TVEPASPTPAH 392

S++WGFV+DVD++SE++R LG RFT+GT LA+L Y+G+L+YLP TV AS PA Sbjct: 290 SLSWGFVADVDLESEKYRRLGEIRFTVGTFFRLASLRIYQGQLAYLPVGTV—ASKRPAS 347

Query: 393 SL-PRAKSELTLTPDPAPPMAH 413

+L + + L P P +H Sbjct: 348 TLVQKGPVDTHLVPLEEPVPSH 369

Score = 324 (48.6 bits), Expect = 2.0e-92, Sum P(2) = 2.0e-92 Identities = 72/160 (45%), Positives = 100/160 (62%)

Query: 499 LPLPTPDARVGASTC GPPDHLLPPLGTPLPPDWVTL-EGDFVLMLAISPΞHLGADLV 554

LP+ T ++ AST GP D L PL P+P W + E DF+L+L + +HL ++L Sbjct: 335 LPVGTVASKRPASTLVQKGPVDTHLVPLEEPVPSHWTVVPEQDFLLVLVLLHTHLSSELF 394

Query: 555 AAPHARFDDGLVHLCWVRSGISRAALLRLFLAMERGSHFSLGCPQLGYAAARAFRLEPLT 614

AAP R + G++HL +VR+G+SRAALLRLFLAM++G H L CP L + AFRLEP + Sbjct: 395 AAPMGRCEAGVMHLFYVRAGVSRAALLRLFLAMQKGKHMELDCPYLVHVPVVAFRLEPRS 454

Query: 615 PRGVLTVDGEQVEYGPLQAQMHPGIGTLLTGPPGCP-GRE 653

RGV +VDGE + +Q Q+HP ++ G P GR+ Sbjct: 455 QRGVFSVDGELMVCEAVQGQVHPNYLWMVCGSRDAPSGRD 494

Score = 37 (5.6 bits), Expect = 3.6e-62, Sum P(2) = 3.6e-62 Identities = 8/20 (40%), Positives = 9/20 (45%)

Query: 459 GAGDAPLSPDPLLSSPPGSP 478

G+ DAP D PP P Sbjct: 485 GSRDAPSGRDSRRGPPPEEP 504

Pedant information for DKFZphfbr2_82m6, frame 3

Report for DKFZphfbr2 82m6.3 [LENGTH] 654

[MW] , 69207.45

[pi] 6.47

[HOMOL] TREMBL:AF068749_1 gene: "SPHKlb"; product: "sphingosine kinase"; Mus musculus sphingosine kinase (SPHKlb) mRNA, complete eds. 2e-50

[FUNCAT] 01.06.01 lipid, fatty-acid and sterol biosynthesis [S. cerevisiae, YLR260w]

4e-20

[PROSITE] AMIDATION 1

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] MYRISTYL 12

[PROSITE] CK2_PHOSPHO_SITE 6

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE 8

[PROSITE] ASN_GLYCOSYLATION 1

[KW] Alpha_Beta

[KW] LOW_COMPLEXITY 20.18 %

SEQ MNGHLEAEEQQDQRPDQELTGSWGHGPRSTLVRAKAMAPPPPPLAASTSLLHGEFGSYPA SEG xxxxxxxxxxxxx

PRD ccchhhhhhhhcccccceeecccccccceeehhhhhccccccceeeceeeeccccccccc

SEQ RGPRFALTLTSQALHIQRLRPKPEARPRGGLVPLAEVSGCCTLRΞRSPSDSAAYFCIYTY SEG

PRD cccceeehhhhhhhhhhhhhccccccccccceeeeeeeceeeeeecccccceeeeeeeec

SEQ PRGRRGARRRATRTFRADGAATYEENRAEAQRWATALTCLLRGLPLPGDGEITPDLLPRP

SEG .xxxxxxxxxxxxxxxxxxxxx xxxxx

PRD ccccchhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhhccccccccccccccccccc

SEQ PRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLSFNLIQTERQNHARELVQGLSLSEWDG SEG xxxxxx

PRD ceeeeeeecccccchhhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhccccce

SEQ IVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGILPCGSGNALAGAVNQHGGFEPALGLDL

SEG xxxxx

PRD eeeecccccceeeccccccccchhhhhccceeeccccccccccccccccccccchhhhhh

SEQ LLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERFRALGSARFTL SEG xxxxxxxxxxxxx

PRD hhhhhhccccccccccceeeeeeccccceeeeeeeeccccceeeehhhhhhhhhhhhhhc

SEQ GTVLGLATLHTYRGRLSYLPATVEPASPTPAHSLPRAKSELTLTPDPAPPMAHSPLHRSV SEG

PRD hhhhhhhhhhhhcccccccccccccccccccccccccccccccccccccccccccccccc

SEQ SDLPLPLPQPALASPGSPEPLPILSLNGGGPELAGDWGGAGDAPLSPDPLLSSPPGSPKA

SEG ..xxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxx

PRD ccccccccccccccccccccceeeeeccccccccccccccccccccccccccccccccce

SEQ ALHSPVΞEGAPVIPPSSGLPLPTPDARVGASTCGPPDHLLPPLGTPLPPDWVTLEGDFVL SEG xx xxxxxxxxxxxxxxx

PRD eeccccccccccccccccccccccccccccccccccccccccccccccccccccccccee

SEQ MLAISPSHLGADLVAAPHARFDDGLVHLCWVRSGISRAALLRLFLAMERGSHFSLGCPQL SEG

PRD eeeeecccccccccccccccccccceeeeeeeccchhhhhhhhhhhhhcccceeecccch

SEQ GYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQMHPGIGTLLTGPPGCPGREP

SEG \ xxxxxxxxxxxxxxx ...

PRD hhhhhhhhhhccccccceeeeccceeecccccccccccccceeecccccccccc

Prosite for DKFZphfbr2_82m6.3

PS00001 303- ->307 ASN GLYCOSYLATION PDOC00001

PS00002 245- ->249 GLYCOSAMINOGLYCAN PDOC00002

PS00004 129- ->133 CAMP PHOSPHO SITE PDOC00004

PS00005 102- ->105 PKC PHOSPHO SITE PDOC00005

PS00005 134- ->137 PKC PHOSPHO SITE PDOC00005

PS00005 220- ->223 PKC PHOSPHO SITE PDOC00005

PS00005 347- ->350 PKC PHOSPHO SITE PDOC00005

PS00005 355- ->358 PKC PHOSPHO SITE PDOC00005

PS00005 371- ->374 PKC PHOSPHO SITE PDOC00005

PS00005 477- ->480 PKC PHOSPHO SITE PDOC00005

PS00005 614- ->617 PKC PHOSPHO SITE PDOC00005

PS00006 107- ->111 CK2 PHOSPHO SITE PDOC00006 o o CO

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DKFZphfkd2_lj9

group: kidney derived

DKFZphfkd2_lj9.3 encodes a novel 105 ammo acid protein with high similarity to Xenopus laevis XLCL2 protein.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of kidney-specific genes . strong similarity to XLCL2 protein, African clawed frog complete cDNA, complete eds, EST hits

Sequenced by LMU

Locus: unknown

Insert length: 2955 bp

Poly A stretch at pos. 2935, polyadenylation signal at pos. 2915

1 GGGGGGGGCT GAGTGCTCAG TGGAGAGCGG GGAGTTGTGT CCACCTTGCC

51 GACGTCGCTA GCCGTGGGGC TGTCCTGGGA AGGCGGACGG CGAGCGCCCG

101 GTGTCCGCAC TCGGCCGCCT GCCGTGCCCG TCTGCGCCCG TGTCATCCTC

151 ACTCGGGACG CAGGGACCGT TTTTAAATCA CAGGGGCGTG TGTCAGCCTG

201 CCCTAGGACT TCATGTCTAT ATATTTCCCC ATTCACTGCC CCGACTATCT

251 GAGATCGGCC AAGATGACTG AGGTGATGAT GAACACCCAG CCCATGGAGG

301 AGATCGGCCT CAGCCCCCGC AAGGATGGCC TTTCCTACCA GATCTTCCCA

351 GACCCGTCAG ATTTTGACCG CCGCTGCAAA CTGAAGGACC GTCTGCCCTC

401 CATAGTGGTG GAACCCACAG AAGGGGAGGT GGAGAGCGGG GAGCTCCGGT

451 GGCCCCCTGA GGAGTTCCTG GTCCAGGAGG ATGAGCAAGA TAACTGCGAA

501 GAGACAGCGA AAGAAAATAA AGAGCAGTAG AGTCCCTGTG GACTCCCATG

551 GGTCATACCA GCCAGCATCT GTTCCTGAAC TGTGTTTTTC CCATCATGAC

601 GGAAGAAGAG AGTGAGCCGC AATTGTTCTG AAAATGTCAA ACGAGGCTTC

651 TGTTTTGCAC CTGCAGATCA CCGAGTTGGT TTTCTTTTCT TTTCTTGCCT

701 TTTTTTTTTT TTTGAAATTT GCCGAGCAGT GGAGCCCTCT GACAATTTGC

751 AAGGCCCTCT GAGAAAGGAA GCTGCTTAGA GCCAGGGGGT TAGTGGGTGA

801 GGGGAGCGAG TGCTGTTTTT GAGATCATTA TCTGAACTCA GGCAGCCTAG

851 TAGAGGCAGT GGTGGGATTC CAATGGGTCT TGGTGGGTGG GAGGTGGGGC

901 ATGTGCAAAG CAAGCAAGGA ACATTTGGGG TAAGAAAACA AACATGAGGC

951 AAAAGAAAAA ATACATGTTT TTAAGAAAAC ATTGAGCAGA GAACTGCAGC

1001 CAGGATGCGC TCAGCAGACA TTCACTCTGG CCGCTGGGAC ATCAGAAAAC

1051 AAAGTCTTCA TCTCTCTCTC CAGTTTCACC CACCCCACCC TTTGCTTTCA

1101 TTTCAGGTGT GTTGGTCTAT ATGACAGGGA GGAGAGTAAA GGAGAGCAGG

1151 AGCAATTGGC TGCCTGCAAA GCCAGCTGGA GGTGAAGTGC AGGAAAGGAA

1201 AGGTCACCCC ATTCTACTCC ATGGCCTCTC TGCTCCCAGC TGTGGTAGGC

1251 TCACATAGCC AGTGTGATCG GTTTTTAAGA GGCAGTGCTT TTCAGCTTTT

1301 CTCCCTGATA TATCCATTTT GCTTCCCAGC ACTTTTTAGG AGTAGTGAGA

1351 GCACTTCCTG CCCTTGTTGG AAGCCCCAGG GTGGACACTC AGCACGAAGG

1401 TCTCTCCCTT AACTGCTGCC CTTCCAAGAC TTGCTCCCGA GATGGAGTGG

1451 GCGTGGTCTT CCAGGCTGGC CCTTCCTTCT CCTCACCGCC ACCTTCCCTG

1501 CCCCAGCCCC AGCAGCCATG GGTACATGGG TCCCCAGCTC ACCTATGGAT

1551 TCCCGCCAGT CTGCCCAGCT GCAGTACTCA CGCCCCATGG GGGATCTTGG

1601 TCTGTTTTTC TTGTGGGAGC CTAGTGGAGA GCAGACGTGG CTTTTTATGT

1651 GTCTTGTTGG GGAGGTGACT TGCATGGTGG GGACAAGGCT GTCGTGGCAA

1701 CCTTGGGATC GAGTTTGAGA CTAAAGGATG TCATGAGATC CCTGGCTTCT

1751 CCCCATGTTG TTCCCGGACA AGGGCAGAAG GGAGGCATGG CAAGGGACCT

1801 CTGCTGTCCT TACTCAACAG TGGTCCTCAT CCCTCCCCAC CTCCCACTGC

1851 TTCCTGCAAG GGCACCAGTT GTATGAGAAA GTTGGCCTTT GGACTTAGGA

1901 TTTCTTATTG TAGCTAAGAG CCATCTGAAG CAGCAGGTTG CAGGACAAAT

1951 GCTTCAGTCC GCCGAGAGCA GTACCGTGTG GCCAAGAGGT GGACTCAGAG

2001 CCTTCCTTGA GCTAAACTCG GCCAACCAAG GCACGCAGCA TGTCCCCTCA

2051 GGTCTCCAGT CAGTCCAGGT TGACCCTCAG TTCTGGACGT GTGTATATAG

2101 CTGTATTTAA TACCTCAAGG TCATTGTGGC TCTGGGGATG CCAGGGCAGG

2151 AGGACGAGGG TGCGCTGTGG ACACAGCAGT CCGCGGAATT CCGTTCTGGG

2201 AAGCCAATGG TCGCCGGCAC CCCTTGCTTC CTCCCTCTGT TGTCTGCCTG

2251 TGTGACACAC ATCAATGGCA ATAACTTCTT CCAACTCCTC GCAGAAGTGG

2301 GAGAGGCCGG CAGCCTGCAC CGAGAGGGGC TTTCCTCTCT CTTGCTCCCC

2351 GCTTCGTTCT GTTTTGGCTG CAGAGAGTGG TTCATCCATA CTCTCATTCC

2401 CTCGCCTCCC CTTGTGGACG GGGGTCTTGC CTTTTCAATT CCTGTGTTTT

2451 GGTGTCTTCC CTTATCTGCT ACCCTGAATC ACCTGTCCTG GTCTTGCTGT

2501 GTGATGGGAA CATGCTTGTA AACTGCGTAA CAAATCTACT TTGTGTATGT

2551 GTCTGTTTAT GGGGGTGGTT TATTATTTTT GCTGGTCCCT AGACCACTTT

2601 GTATGACCGT TTGCAGTCTG AGCAGGCCAG GGGCTGACAG CTAATGTCAG

2651 GACCCTCAGC GGTGGAGCCT GCTGGGGGGA CCCAGCTGCT CTTGGACAAG 2701 TGGCTGAGCT CCTATCTGGC CTCCTCTTTT CAAGTAATTT 2751 GTGTGTATTT CTAACTGATT GTATTGAAAA AATTCCTAGT ATTTCAGTAA 2801 AAATGCCTGT TGTGAGATGA ACCTCCTGTA ACTTCTATCT GTTCTTTTTT 2851 GAGGCTCAGG GAGAAACTAG CATTTTTTTT TTTCCAAACT ACTTTTTGTC 2901 ACTGTGACAG TTGTAAATAA AGTTTGAAAA TGCTCAAAAA AAAAAAAAAA 2951 AAAAC

BLAST Results

Entry HSG19750 from database EMBL: human STS A001X24. Score = 1050, P = 1.9e-39, identities = 212/213

Entry HSG20267 from database EMBL: human STS A005C12. Score = 610, P = 4. le-19, identities = 122/122

Medline entries

No Medlme entry

Peptide information for frame 3

ORF from 213 bp to 527 bp; peptide length: 105 Category: strong similarity to known protein Classification: unset

1 MSIYFPIHCP DYLRSAKMTE VMMNTQPMEE IGLSPRKDGL SYQIFPDPSD 51 FDRRCKLKDR LPSIVVEPTE GEVESGELRW PPEEFLVQED EQDNCEETAK 101 ENKEQ

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfkd2_lj 9, frame 3

PIR:S52241 XLCL2 protein - African clawed frog, N = 1, Score = 443, P = 8e-42

PIR:S52241 XLCL2 protein - African clawed frog, N = 1, Score = 443, P = 8.2e-42

>PIR:S52241 XLCL2 protein - African clawed frog Length = 102

HSPs:

Score = 443 (66.5 bits), Expect = 8.0e-42, P = 8.0e-42 Identities = 80/104 (76%), Positives = 95/104 (91%)

Query: 1 MSIYFPIHCPDYLRSAKMTEVMMNTQPMEEIGLSPRKDGLSYQIFPDPSDFDRRCKLKDR 60

MS+++PIHC DYLRSA+MTEV+MNTQ M+EIGLSPRKD SYQIFPDPSDF+R CKLKDR Sbjct: 1 MSVFYPIHCTDYLRSAEMTEVIMNTQSMDEIGLSPRKD—SYQIFPDPSDFERCCKLKDR 58

Query: 61 LPSIVVEPTEGEVESGELRWPPEEFLVQEDEQDNCEETAKENKE 104

LPSIVVEPTEG+VESGELRWPPEEF+V ED++ C++T KEN++ Sbjct: 59 LPSIVVEPTEGDVESGELRWPPEEFVVDEDKEGTCDQTKKENEQ 102

Pedant information for DKFZphfkd2_lj 9, frame 3

Report for DKFZphf d2_lj 9.3

[LENGTH] 105

[MW] 12269.78

[pi] 4.40

[HOMOL] PIR:S52241 XLCL2 protein - African clawed frog 5e-44 [KW] Alpha_Beta

SEQ MSIYFPIHCPDYLRSAKMTEVMMNTQPMEEIGLSPRKDGLSYQIFPDPSDFDRRCKLKDR

PRD cccccccccccchhhhhhhhhhhhcccccccccccccccceeeecccccccchhhhhhhc

SEQ LPSIVVEPTEGEVESGELRWPPEEFLVQEDEQDNCEETAKENKEQ

PRD ccceeeecccccccccccccccccceeeccccchhhhhhhhhccc

(No Prosite data available for DKFZphfkd2_l 9.3) (No Pfam data available for DKFZphfkd2_lj9.3)

DKFZphfkd2_24al5

group: transmembrane protein

DKFZphfkd2_24al5 encodes a novel amino acid protein with similarity to C. elegans cosmid R07G3.

The novel protein contains 1 transmembrane region.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of kidney-specific genes and as a new marker for kidney cells.

similarity to C. elegans R07G3.8 membrane regions : 1

Summary DKFZphfkd2_24al5 encodes a novel 323 ammo acid protein, with similarity to C. elegans R07G3.8.

similarity to C. elegans R07G3.8 complete cDNA, complete eds, EST hits

Sequenced by GBF

Locus: unknown

Insert length: 1513 bp

Poly A stretch at pos. 1494, no polyadenylation signal found

1 GGGGTACTCG GCGGCGGCGG AGCGGGCGGC AGAGCAGGGC GGCGGCGACT

51 CGCAGGGTAC CACCATCTTA AGGACAGAAA AGCTACAGGA CTCTAGGAGG

101 CCACCGTCCT GATTTGGGAA GTCCAACTTA CTTTGGCCAG ACAGCAGCTA

151 AGCTGGTTCA TCCCATCAGC CTGGATTGGT GAAACTGAAT CACAGGAGAT

201 ATTTCCAGGT TTGCTGGGAT GGGAAACCTG CTCAAAGTCC TTACCAGGGA

251 AATTGAAAAC TATCCACACT TTTTCCTGGA TTTTGAAAAT GCTCAGCCTA

301 CAGAAGGAGA GAGAGAAATC TGGAACCAGA TCAGCGCCGT CCTTCAGGAT

351 TCTGAGAGCA TCCTTGCAGA CCTGCAGGCT TACAAAGGCG CAGGCCCAGA

401 GATCCGAGAT GCAATTCAAA ATCCCAATGA CATTCAGCTT CAAGAAAAAG

451 CTTGGAATGC GGTGTGCCCT CTTGTTGTGA GGCTAAAGAG ATTTTACGAG

501 TTTTCCATTA GACTAGAAAA AGCTCTTCAG AGTTTATTGG AATCTCTGAC

551 TTGTCCACCC TACACACCAA CCCAACACCT GGAAAGGGAA CAGGCCCTGG

601 CAAAGGAGTT TGCCGAAATT TTACATTTTA CCCTTCGATT CGATGAGCTG

651 AAGATGAGGA ACCCGGCTAT TCAGAATGAC TTCAGCTACT ACAGAAGAAC

701 AATCAGTCGC AACCGCATCA ACAACATGCA CCTAGACATT GAGAATGAAG

751 TCAATAATGA GATGGCCAAT CGAATGTCCC TCTTCTATGC AGAAGCCACG

801 CCAATGCTGA AAACCCTTAG CAATGCCACA ATGCACTTTG TCTCTGAAAA

851 CAAAACTCTG CCAATAGAGA ACACCACAGA CTGCCTCAGC ACAATGACAA

901 GTGTCTGTAA AGTCATGCTG GAAACTCCGG AGTACAGAAG TAGGTTTACG

951 AGTGAAGAGA CCCTGATGTT CTGCATGAGG GTGATGGTGG GAGTCATCAT

1001 CCTCTATGAC CATGTCCACC CTGTGGGAGC TTTCTGCAAG ACATCCAAGA

1051 TCGATATGAA AGGCTGCATA AAAGTTTTGA AGGAGCAGGC CCCAGACAGT

1101 GTGGAGGGGC TGCTAAATGC CCTCAGGTTC ACTACAAAGC ACTTGAACGA

1151 TGAATCAACT TCCAAACAGA TTCGAGCAAT GCTTCAGTAG AGCTCTGCTC

1201 AAAGAAGAGG ATCTATGTGC TGACCTCAGA AGATGTATAT GTTTACATAA

1251 TTTAATACAG ATTGATGTTA ATACTTGTGT ATTTACATAA CCGTTTCCTT

1301 CTTGTCACTG AAATATATGG ACCTTAATTT GTATCCTGAC TGACTCAACC

1351 CAGCAGAGCA TAAATTGACT TGAGAGCCTT ACCTTTGATG TCTGAAATGA

1401 AACCCCCTTC TCCAAAGGCA AAATTCGGAG ACTTTGATCT TTGCTACTGG

1451 AGTCCTTTAA CAACATCTAT AACGATAAAA AATTCCTAAT TGTCAAAAAA

1501 AAAAAAAAAA AAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 3 ORF from 219 bp to 1187 bp; peptide length: 323 Category: similarity to unknown protein

1 MGNLLKVLTR EIENYPHFFL DFENAQPTEG EREIWNQISA VLQDSESILA

51 DLQAYKGAGP EIRDAIQNPN DIQLQEKAWN AVCPLVVRLK RFYEFSIRLE

101 KALQSLLESL TCPPYTPTQH LEREQALAKE FAEILHFTLR FDELKMRNPA

151 IQNDFSYYRR TISRNRINNM HLDIENEVNN EMANRMSLFY AEATPMLKTL

201 SNATMHFVSE NKTLPIENTT DCLSTMTSVC KVMLETPEYR SRFTSEETLM

251 FCMRVMVGVI ILYDHVHPVG AFCKTSKIDM KGCIKVLKEQ APDSVEGLLN

301 ALRFTTKHLN DESTSKQIRA MLQ

BLASTP hits

Entry CER07G3_7 from database TREMBL: gene: "R07G3.8"; Caenorhabditis elegans cosmid R07G3.

Score = 544, P = 1.4e-52, identities = 119/323, positives 186/323

Alert BLASTP hits for DKFZphfkd2_24al5, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphfkd2_24al5, frame 3

Report for DKFZphfkd2_24al5.3

[LENGTH] 323 [MW] 37313.06 [pi] 5.71 [HOMOL] TREMBL :CER07G3 7 gene: "R07G3.8"; Caenorhabditis elegans cosmid R07G3. 4e-54

[PROSITE] MYRISTYL 1 [PROSITE] CK2_PHOSPHO_SITE 4 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 5 [PROSITE] ASN_GLYCOSYLATION 3 [KW] TRANSMEMBRANE 1

SEQ MGNLLKVLTREIENYPHFFLDFENAQPTEGEREIWNQISAVLQDSESILADLQAYKGAGP PRD ccccchhhhhhhhcccceeecccccccchhhhhhhhhhhhhhhcchhhhhhhhhhccccc MEM

SEQ EIRDAIQNPNDIQLQEKAWNAVCPLVVRLKRFYEFSIRLEKALQSLLESLTCPPYTPTQH PRD hhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccchhh

MEM

SEQ LEREQALAKEFAEILHFTLRFDELKMRNPAIQNDFSYYRRTISRNRINNMHLDIENEVNN PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccchhhhhhccchhhhhhhhhhhhhhhh MEM

SEQ EMANRMSLFYAEATPMLKTLSNATMHFVSENKTLPIENTTDCLSTMTSVCKVMLETPEYR PRD hhhhhhhhhhhhccchhhhhhhhceeecccccccccccccceeeeehhhhhhhhcccccc MEM

SEQ SRFTSEETLMFCMRVMVGVIILYDHVHPVGAFCKTSKIDMKGCIKVLKEQAPDSVEGLLN PRD cccccchhhhhhhhhhhheeeeeeeccccccccccccccchhhhhhhhhccccchhhhhh MEM MMMMMMMMMMMMMMMMMMMMM

SEQ ALRFTTKHLNDESTSKQIRAMLQ PRD hhhhhhcccccccchhhhhhccc MEM

Prosite for DKFZphfkd2_24al5.3

PS00001 202->206 ASN_GLYCOSYLATION PDOC00001 PS00001 211->215 ASN_GLYCOSYLATION PDOC00001 PS00001 218->222 ASN_GLYCOSYLATION PDOC00001 PS00005 96->99 PKC_PHOSPHO_SITE PDOC00005 PS00005 138->141 PKC_PHOSPHO_SITE PDOC00005 PS00005 275->278 PKC_PHOSPHO_SITE PDOC00005 PS00005 305->308 PKC PHOSPHO SITE PDOC00005 PS00005 314->317 PKC_PHOSPHO_SITE PDOC00005 PS00006 28->32 CK2_PHOSPHO_SITE PDOC00006 PS00006 105->109 CK2_PHOSPHO_SITE PDOC00006 PS00006 244->248 CK2_PHOSPHO_SITE PDOC00006 PS00006 276->280 CK2_PHOSPHO_SITE PDOC00006 PS00007 231->240 TYR_PHOSPHO_SITE PDOC00007 PS00008 297->303 MYRISTYL PDOC00008

(No Pfam data available for DKFZphf d2_24al5.3)

DKFZphfkd2_24bl5

group: metabolism

DKFZphfkd2_24bl5 encodes a novel 612 ammo acid protein with similarity to bacterial and yeast phosphoglucomutase and phosphomannomutases.

The novel protein contains a phosphoserme signature typical for phosphoglucomutase (EC 5.4.2.2) or phosphomannomutase (EC 5.4.2.8). Thus, the protein seems to be taking part in the conversion of hexose phosphates.

The new protein can find application in modulation of hexose metabolism pathways and as a new enzyme for biotechnologic production processes. similarity to phosphomannomutases complete cDNA, complete eds, EST hits potential start at bp 30 matches kozak consensus PyCNatgG,

Sequenced by GBF

Locus: map="158.8 cR from top of Chr4 linkage group"

Insert length: 2204 bp

Poly A stretch at pos. 2186, no polyadenylation signal found

1 GGGCTCTGCA GCGGTAGCAC AAGCTCAGCG ATGGCGGCTC CAGAAGGCAG

51 CGGTCTAGGC GAGGACGCCC GGCTGGACCA GGAGACCGCC CAGTGGCTGC

101 GCTGGGACAA GAATTCCTTA ACTTTGGAGG CAGTGAAACG ACTAATAGCA

151 GAAGGTAATA AAGAAGAACT ACGAAAATGT TTTGGGGCCC GAATGGAGTT

201 TGGGACAGCT GGCCTCCGAG CTGCTATGGG ACCTGGAATT TCTCGTATGA

251 ATGACTTGAC CATCATCCAG ACTACACAGG GATTTTGCAG ATACCTGGAA

301 AAACAATTCA GTGACTTAAA GCAGAAAGGC ATCGTGATCA GTTTTGACGC

351 CCGAGCTCAT CCATCCAGTG GGGGTAGCAG CAGAAGGTTT GCCCGACTTG

401 CTGCAACCAC ATTTATCAGT CAGGGGATTC CTGTGTACCT CTTTTCTGAT

451 ATAACGCCAA CCCCCTTTGT GCCCTTCACA GTATCACATT TGAAACTTTG

501 TGCTGGAATC ATGATAACTG CATCTCACAA TCCAAAGCAG GATAATGGTT

551 ATAAGGTCTA TTGGGATAAT GGAGCTCAGA TCATTTCTCC TCACGATAAA

601 GGGATTTCTC AAGCTATTGA AGAAAATCTA GAACCGTGGC CTCAAGCTTG

651 GGACGATTCT TTAATTGATA GCAGTCCACT TCTCCACAAT CCGAGTGCTT

701 CCATCAATAA TGACTACTTT GAAGACCTTA AAAAGTACTG TTTCCACAGG

751 AGCGTGAACA GGGAGACAAA GGTGAAGTTT GTGCACACCT CTGTCCATGG

801 GGTGGGTCAT AGCTTTGTGC AGTCAGCTTT CAAGGCTTTT GACCTTGTTC

851 CTCCTGAGGC TGTTCCTGAA CAGAGAGATC CGGATCCTGA GTTTCCAACA

901 GTGAAATACC CGAATCCCGA AGAGGGGAAA GGTGTCTTGA CTTTGTCTTT

951 TGCTTTGGCT GACAAAACCA AGGCCAGAAT TGTTTTAGCT AACGACCCGG

1001 ATGCTGATAG ACTTGCTGTG GCAGAAAAGC AAGACAGTGG TGAATGGAGG

1051 GTGTTTTCAG GCAATGAGTT GGGGGCCCTC CTGGGCTGGT GGCTTTTTAC

1101 ATCTTGGAAA GAGAAGAACC AGGATCGCAG TGCTCTCAAA GACACGTACA

1151 TGTTGTCCAG CACCGTCTCC TCCAAAATCT TGCGGGCCAT TGCCTTAAAG

1201 GAAGGTTTTC ATTTTGAGGA AACATTAACT GGCTTTAAGT GGATGGGAAA

1251 CAGAGCCAAA CAGCTAATAG ACCAGGGGAA AACTGTTTTA TTTGCATTTG

1301 AAGAAGCTAT TGGATACATG TGCTGCCCTT TTGTTCTGGA CAAAGATGGA

1351 GTCAGTGCCG CTGTCATAAG TGCAGAGTTG GCTAGCTTCC TAGCAACCAA

1401 GAATTTGTCT TTGTCTCAGC AACTAAAGGC CATTTATGTG GAGTATGGCT

1451 ACCATATTAC TAAAGCTTCC TATTTTATCT GCCATGATCA AGAAACCATT

1501 AAGAAATTAT TTGAAAACCT CAGAAACTAC GATGGAAAAA ATAATTATCC

1551 AAAAGCTTGT GGCAAATTTG AAATTTCTGC CATTAGGGAC CTTACAACTG

1601 GCTATGATGA TAGCCAACCT GATAAAAAAG CTGTTCTTCC CACTAGTAAA

1651 AGCAGCCAAA TGATCACCTT CACCTTTGCT AATGGAGGCG TGGCCACCAT

1701 GCGCACCAGT GGGACAGAGC CCAAAATCAA GTACTATGCA GAGCTGTGTG

1751 CCCCACCTGG GAACAGTGAT CCTGAGCAGC TGAAGAAGGA ACTGAATGAA

1801 CTGGTCAGTG CTATTGAAGA ACATTTTTTC CAGCCACAGA AGTACAATCT

1851 GCAGCCAAAA GCAGACTAAA ATAGTCCAGC CTTGGGTATA CTTGCATTTA

1901 CCTACAATTA AGCTGGGTTT AACTTGTTAA GCAATATTTT TAAGGGCCAA

1951 ATGATTCAAA ACATCACAGG TATTTATGTG TTTTACAAAG ACCTACATTC

2001 CTCATTGTTT CATGTTTGAC CTTTAAGGTG AAAAAAGAAA ATGGCCAAAC

2051 CCAACAAACT AACATTCCTA CTAAAAAGTT GAGCTTGGAC ATATTTTGAA

2101 TTTTTGTAAG TGAAGATTTT TAAACTGACT AACTTAAAAA AATAGATTGT

2151 AATTGATGTG CCTTAATTTG CATAAATCAT AAATGTAAAA AAAAAAAAAA

2201 AAAA

BLAST Results

Entry HS705145 from database EMBL: human STS WI-6820. Score = 1261, P = 3.6e-52, identities = 253/254

Medline entries

No Medline entry

Peptide information for frame 1

ORF from 31 bp to 1866 bp; peptide length: 612 Category: strong similarity to known protein

1 MAAPEGSGLG EDARLDQETA QWLRWDKNSL TLEAVKRLIA EGNKEELRKC

51 FGARMEFGTA GLRAAMGPGI SRMNDLTIIQ TTQGFCRYLE KQFSDLKQKG

101 IVISFDARAH PSSGGSSRRF ARLAATTFIS QGIPVYLFSD ITPTPFVPFT

151 VSHLKLCAGI MITASHNPKQ DNGYKVYWDN GAQIISPHDK GISQAIEENL

201 EPWPQAWDDS LIDSSPLLHN PSASINNDYF EDLKKYCFHR SVNRETKVKF

251 VHTSVHGVGH SFVQSAFKAF DLVPPEAVPE QRDPDPEFPT VKYPNPEEGK

301 GVLTLSFALA DKTKARIVLA NDPDADRLAV AEKQDSGEWR VFSGNELGAL

351 LGWWLFTSWK EKNQDRSALK DTYMLSSTVS SKILRAIALK EGFHFEETLT

401 GFKWMGNRAK QLIDQGKTVL FAFEEAIGYM CCPFVLDKDG VSAAVISAEL

451 ASFLATKNLS LSQQLKAIYV EYGYHITKAS YFICHDQETI KKLFENLRNY

501 DGKNNYPKAC GKFEISAIRD LTTGYDDSQP DKKAVLPTSK SSQMITFTFA

551 NGGVATMRTΞ GTEPKIKYYA ELCAPPGNSD PEQLKKELNE LVSAIEEHFF

601 QPQKYNLQPK AD

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfkd2_24bl5, frame 1

TREMBL :CEY43F4B_5 gene: "Y43F4B.5"; Caenorhabditis elegans cosmid Y43F4B, N = 1, Score = 1431, P = 1.6e-146

TREMBL :SPCC1840_5 gene: "SPCC1840.05c"; product: "similarity to phosphomannomutases"; S. pombe chromosome III cosmid cl840., N = 1, Score = 1210, P = 4.2e-123

PIR:S54585 hypothetical protein YMR278w - yeast (Saccharomyces cerevisiae), N = 1, Score = 1046, P = le-105

PIR:A71299 probable phosphomannomutase (manB) - syphilis spirochete, N = 1, Score = 697, P = 9.7e-69

>TREMBL:CEY43F4B_5 gene: "Y43F4B.5"; Caenorhabditis elegans cosmid Y43F4B Length = 595

HSPs:

Score = 1431 (214.7 bits), Expect = 1.6e-146, P = 1.6e-146 Identities = 285/598 (47%), Positives = 393/598 (65%)

Query: 13 ARLDQETAQWLRWDKNΞLTLEAVKRLIAEGNKEELRKCFGARMEFGTAGLRAAMGPGIΞR 72

A+LD++ A WL WDKN +++L+ E N + L+ R+ FGTAG+R+ M G R Sbjct: 6 AKLDKQVADWLAWDKNDKNRNEIQKLVDEKNVDALKARMDTRLVFGTAGVRSPMQAGFGR 65

Query: 73 MNDLTIIQTTQGFCRYLEKQFSDLKQKGIVISFDARAHPSSGGSSRRFARLAATTFISQG 132

+NDLTIIQ T GF R++ + K G+ I FD R + SRRFA L+A F+ Sbjct: 66 LNDLTIIQITHGFARHMLNVYGQPKN-GVAIGFDGRYN ΞRRFAELSANVFVRNN 118

Query: 133 IPVYLFSDITPTPFVPFTVSHLKLCAGIMITAΞHNPKQDNGYKVYWDNGAQIISPHDKGI 192

IPVYLFS+++PTP V + L AG++ITASHNPK+DNGYK YW NGAQII PHD I Sbjct: 119 IPVYLFSEVSPTPVVSWATIKLGCDAGLIITASHNPKEDNGYKAYWSNGAQIIGPHDTEI 178

Query: 193 SQAIEENLEPWPQAWDDSLIDSSPLLHNPSASINNDYFEDLKKYCFHRSVNRETKVKFVH 252

+ E +P + WD S + SSPL H+ 1+ YFE K F R +N T +KF + Sbjct: 179 VRIKEAEPQPRDEYWDLSELKSSPLFHSADVVID-PYFEVEKSLNFTREINGSTPLKFTY 237

Query: 253 TSVHGVGHSFVQSAFKAFDLVPPE—AVPEQRDPDPEFPTVKYPNPEEGKGVLTLSFALA 310

++ HG+G+ + + F F +V EQ+DP+P+FPT+ +PNPEEG+ VLTL+ A Sbjct: 238 SAFHGIGYHYTKRMFAEFGFPASΞFISVAEQQDPNPDFPTIPFPNPEEGRKVLTLAMETA 297 Query: 311 DKTKARIVLANDPDADRLAVAEKQDSGEWRVFSGNELGALLGWWLFTSWKEKNQDRSALK 370

DK + ++LANDPDADR+ +AEKQ GEWRVF+GNE+GAL+ WW++T+W++ N + A K Sbjct: 298 DKNGSTVILANDPDADRIQMAEKQKDGEWRVFTGNEMGALITWWIWTNWRKANPNADASK 357 Query: 371 DTYMLSSTVSSKILRAIALKEGFHFEETLTGFKWMGNRAKQLIDQGKTVLFAFEEAIGYM 430

Y+L+S VSS+I++ IA EGF E TLTGFKWMGNRA++L G V+ A+EE+IGYM Sbjct: 358 -VYILNSAVSSQIVKTIADAEGFKNETTLTGFKWMGNRAEELRADGNQVILAWEESIGYM 416 Query: 431 CCP-FVLDKDGVSAAVISAELASFLATKNLSLSQQLKAIYVEYGYHITKASYFICHDQET 489

P +DKDGVSAA + AE+A+FL + SL QL A+Y YG+H+ +++Y++ E Sbjct: 417 —PGHTMDKDGVSAAAVFAEIAAFLHAEGKSLQDQLYALYNRYGFHLVRSTYWMVPAPEV 474 Query: 490 IKKLFENLRNYDGKNNYPKACGKFEISAIRDLTTGYDDSQPDKKAVLPTSKSSQMITFTF 549

KKLF LR D K +P G+ E++++RDLT GYD+S+PD K VLP S SS+M+TF Sbjct: 475 TKKLFSTLRA-DLK—FPTKIGEAEVASVRDLTIGYDNSKPDNKPVLPLSTSSEMVTFFL 531 Query: 550 ANGGVATMRTSGTEPKIKYYAELCAPPGNS—DPEQLKKELNELVSAIEEHFFQPQKYNL 607

G V T+R SGTEPKIKYY EL PG + D E + E+++L + +PQ++ L Sbjct: 532 KTGSVTTLRASGTEPKIKYYIELITAPGKTQNDLESVISEMDQLEKDVVATLLRPQQFGL 591 Query: 608 QPK 610

P+ Sbjct: 592 IPR 594

Pedant information for DKFZphfkd2_24bl5, frame 1

Report for DKFZphfkd2_24bl5.1

[LENGTH] 612 [MW] 68311.58 [pi] 6.28 [HOMOL] TREMBL :CEY43F4B_5 gene: "Y43F4B.5"; Caenorhabditis elegans cosmid Y43F4B le-157

[FUNCAT] 01.05.01 carbohydrate utilization [S. cerevisiae, YMR278w] le-111

[FUNCAT] g carbohydrate metabolism and transport [H. influenzae, HI0740] 3e-66

[FUNCAT] c energy conversion [M. genitalium, MG053] 4e-50

[FUNCAT] m outer membrane and cell wall [H. influenzae, HI1463] 2e-04

[BLOCKS] BL00607D cAMP phosphodiesterases class-II proteins

[BLOCKS] BL00710 Phosphoglucomutase and phosphomannomutase phosphoserme signa

[EC] 5.4.2.8 Phosphomannomutase 3e-56

[EC] 5.4.2.2 Phosphoglucomutase le-09

[PIRKW] lsomerase 3e-56

[PIRKW] intramolecular transferase 3e-56

[SUPFAM] Methanobacteπum thermoautotrophicum phosphomannomutase le-06

[SUPFAM] probable phosphorylating protein ureC 9e-06

[PROSITE] PGM_PMM 1

[PROSITE] MYRISTYL 10

[PROSITE] LIPOCALIN 2

[PROSITE] CK2_PHOSPHO_SITE 9

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE 8

[PROSITE] ASN_GLYCOSYLATION 1

[PFAM] Phosphoglucomutase and phosphomannomutase phosphoserme

[KW] Alpha_Beta

SEQ MAAPEGSGLGEDARLDQETAQWLRWDKNΞLTLEAVKRLIAEGNKEELRKCFGARMEFGTA PRD ccccccccccchhhhhhhhhhhhhhhccchhhhhhhhhhhhcchhhhhhhhhhhhccccc

SEQ GLRAAMGPGISRMNDLTIIQTTQGFCRYLEKQFSDLKQKGIVISFDARAHPSSGGSSRRF PRD cccccccccccccceeeeeehhhhhhhhhhhhcccccceeeeeecccccccccccchhhh

SEQ ARLAATTFISQGIPVYLFSDITPTPFVPFTVSHLKLCAGIMITASHNPKQDNGYKVYWDN PRD hhhhhhhhhhccceeeeeccccccccchhhhhhhcccceeeeeeccccccccceeeeecc

SEQ GAQIISPHDKGISQAIEENLEPWPQAWDDSLIDSSPLLHNPSASINNDYFEDLKKYCFHR PRD ccccccccchhhhhhhhhhhhhhcccccccccccccccccccchhhhhhhhhhhhhhhcc

SEQ SVNRETKVKFVHTSVHGVGHSFVQSAFKAFDLVPPEAVPEQRDPDPEFPTVKYPNPEEGK PRD ccccccceeeeeeeccccccchhhhhhhhhcccccccccccccccccccccccccccchh

SEQ GVLTLSFALADKTKARIVLANDPDADRLAVAEKQDSGEWRVFSGNELGALLGWWLFTSWK PRD hhhhhhhhhhhhhcceeeeeccccccceeeeecccccceeeecccchhhhhhhhhhhhhh

SEQ EKNQDRSALKDTYMLSSTVSSKILRAIALKEGFHFEETLTGFKWMGNRAKQLIDQGKTVL PRD hcccccccccceeeeeeeehhhhhhhhhhhcccceeeeeccccchhhhhhhhhhccceee SEQ FAFEEAIGYMCCPFVLDKDGVSAAVISAELASFLATKNLSLSQQLKAIYVEYGYHITKAS PRD hhhhhccccccccccccccchhhhhhhhhhhhhhhhhccchhhhhhhhhhhhcccccccc

SEQ YFICHDQETIKKLFENLRNYDGKNNYPKACGKFEISAIRDLTTGYDDSQPDKKAVLPTSK PRD eeeccchhhhhhhhhhhhhhhcccccccccchhhhhhhcccccccccccccccccccccc

SEQ SSQMITFTFANGGVATMRTSGTEPKIKYYAELCAPPGNSDPEQLKKELNELVSAIEEHFF PRD ccceeeeeecccceeeeecccccccceeeeeeccccccchhhhhhhhhhhhhhhhhhhhh

SEQ QPQKYNLQPKAD PRD cccccccccccc

Prosite for DKFZphfkd2_24bl5.1

PS00001 458->462 ASNJ3LYCOSYLATION PDOC00001 PS00002 7->ll GLYCOSAMINOGLYCAN PDOC00002 PS00005 116->119 PKC_PHOSPHO_SITE PDOC00005 PS00005 117->120 PKC_PHOΞPHO_SITE PDOC00005 PS00005 290->293 PKC_PHOSPHO_SITE PDOC00005 PS00005 358->361 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 380->383 PKC_PHOSPHO_SITE PDOC00005 PS00005 489->492 PKC_PHOSPHO_SITE PDOC00005 PS00005 538->541 PKC_PHOΞPHO_SITE PDOC00005 PS00005 556->559 PKC_PHOSPHO_SITE PDOC00005 PS00006 186->190 CK2_PHOSPHO_SITE PDOC00006 PS00006 210->214 CK2_PHOSPHO_SITE PDOC00006 PS00006 343->347 CK2_PHOSPHO_SITE PDOC00006 PS00006 358->362 CK2_PHOSPHO_SITE PDOC00006 PS00006 523->527 CK2_PHOSPHO_SITE PDOC00006 PS00006 528->532 CK2_PHOSPHO_SITE PDOC00006 PS00006 560->564 CK2_PHOSPHO_SITE PDOC00006 PS00006 579->583 CK2_PHOSPHO_SITE PDOC00006 PS00006 593->597 CK2_PHOSPHO_SITE PDOC00006 PS00008 6->12 MYRISTYL PDOC00008 PS00008 61->67 MYRISTYL PDOC00008 PS00008 100->106 MYRISTYL PDOC00008 PS00008 159->165 MYRISTYL PDOC00008 PS00008 191-M97 MYRISTYL PDOC00008 PS00008 257->263 MYRISTYL PDOC00008 PS00008 344->350 MYRISTYL PDOC00008 PS00008 348->354 MYRISTYL PDOC00008 PS00008 440->446 MYRISTYL PDOC00008 PS00008 552->558 MYRISTYL PDOC00008 PS00710 159->174 PGM_PMM PDOC00589 PS00213 346->358 LIPOCALIN PDOC00187 PS00213 344->358 LIPOCALIN PDOC00187

Pfam for DKFZphf d2_24bl5.1

HMM_NAME Phosphoglucomutase and phosphomannomutase phosphoserme

HMM *GvnVIdIGQNGMMPTPMIYFaIRTYKhmcmggGIMITaSHNPGGPDnDN

G+ V + ++PTP + F + H+++ +GIMITASHNP DN

Query 132 GIPVYLFS--DITPTPFVPFTVS HLKLCAGIMITASHNP—KQ-DN 172

HMM GIK*

G+K Query 173 GYK 175 DKFZphfkd2_24e23

group: kidney derived

DKFZphfkd2_24e23 encodes a novel 198 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application m studying the expression profile of kidney-specific genes. unknown complete cDNA, complete eds, 1 EST hit, many ATGs in front of the ORF

Sequenced by GBF

Locus : unknown

Insert length: 1723 bp

Poly A stretch at pos. 1695, no polyadenylation signal found

1 GGGGGATTTT CGATCATGAC AACGATAGCA ATTGATATAC CTTCAAAATA

51 CGTGTCCAGT GAGTGTTGAT TGTGTGTGGT TTCTCTAGGA GACCGTGTTC

101 ATGCAACACA GCATTATTTC ACCGCCTTTA CCCCAGCTTC TTCATACACA

151 TGCACTTGTC AAGGGCTCTT TGGCTGAAGA GAAGTTAGAA GTTTCCAGAT

201 ATGGAGGGGT ATTTTCAGCA GATATGCCCA CCGCCATGGT TTTGTCAGCT

251 CTGTAGGGTG GTCTTGCACC CTGCTCACTG CTGGCATCAC CTGAGCCTAT

301 GGCAGATACC CAGTGCTGCC CGCCACCATG TGAATTCATC AGCTCTGCAG

351 GCACAGACCT TGCACTAGGA ATGGGCTGGG ACGCCACCCT CTGCCTCTTA

401 CCATTCACTG GGTTTGGCAA GTGTGCTGGG ATCTGGAATC ACATGGATGA

451 GGAACCCGAT AATGGTGACG ACCGAGGTAG CAGGCGAACC ACTGGCCAGG

501 GCAGGAAGTG GGCAGCTCAC GGGACTATGG CTGCACCGCG GGTTCATACC

551 GACTACCATC CTGGAGGTGG GAGCGCATGC TCATCTGTAA AAGTCCGGTC

601 CCACGTTGGA CACACCGGGG TCTTCTTCTT TGTTGACCAG GATCCTCTGG

651 CAGTGTCTTT AACAAGCCAG AGTCTGATCC CACCGCTCAT AAAGCCAGGG

701 TTGTTGAAAG CTTGGGGCTT CCTCCTCCTC TGTGCGCAGC CCTCAGCAAA

751 CGGTCACAGC CTGTGCTGTC TGCTGTACAC CGACTTGGTA TCATCCCATG

801 AACTGTCCCC CTTTCGTGCT CTGTGCTTAG GGCCCTCTGA TGCCCCATCT

851 GCCTGCGCTT CCTGCAACTG TTTAGCAAGC ACCTATTATC TATAGGGTGC

901 TGGGGTGCTG GGCGAGGCCA ATCGCTCCTA TTACTTTCTG CCCTGGGGAC

951 GTCCTGTTTT CCCACCTACC CCTGTAACGC CTCTGCTCTG CCTTCCCATC

1001 TGCGGGGCTA ACGCCATCCC ACAAGGGCTG GGCTGTCCGT TCAGAAGAGA

1051 AACTGGGAAG GGGCCTTGAG GACCTGTGTC CAGGCAGGGT GGACAAGGGC

1101 TTTGTGCAGG GAGCTCCTCT CCCATCTTTG TGTCCTGACA GCCGTGACCG

1151 TGACCCCTCA AAGCAGAGCC AGTAGTGATC AGTATCCTGC TGCTTCAAGC

1201 CTGCACGGTC CTCTTCTCCT CTCCGCACAT CTGCATGCCT GTCAAACCCA

1251 GAGTAGTTTG GGGCCTGGTA AACAGAGGGA AGTTGGCTGG AGGAGGCCAG

1301 TCAGGAGTGC AAGAACCCCG CGTACTCTGT CCCACGTGGA TAAAGTCTCT

1351 AATTCCAGTC TGAGGTGAAT TCTTAGAGAG TGCTTTCATT TAATGTTTGC

1401 TTTATGCATT TCCCCTGCAG CTGTGACTAA TTGTGGAACA GCATACATTT

1451 TGTTTTGAGA CTCTCTTGAG ATTTTTCTGG CAGTGTAAGG TCTACACCAT

1501 TTTCCTCTCA GCATCAGAGA AGGCAGAAAG CAAGAGAAAG GAATGCAATG

1551 TGAGCAAGGC CAGGCACACT TGTGCTACTG CAGTTGGCAA GAATGGAGTC

1601 TAATCCCAGC ACTTTGGGAG GCCGAGGCGG GTGGATCACC TGAGGTCAGG

1651 AATTTGAGAC CAACCTGGCC AACATGTTGA AACCTCGTCT GTACTAAAAA

1701 TACAAAAAAA AAAAAAAAAA AAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2 ORF from 299 bp to 892 bp; peptide length: 198 Category: putative protein

1 MADTQCCPPP CEFISSAGTD LALGMGWDAT LCLLPFTGFG KCAGIWNHMD 51 EEPDNGDDRG SRRTTGQGRK WAAHGTMAAP RVHTDYHPGG GSACSSVKVR 101 SHVGHTGVFF FVDQDPLAVS LTSQSLIPPL IKPGLLKAWG FLLLCAQPSA 151 NGHSLCCLLY TDLVSSHELS PFRALCLGPS DAPSACASCN CLASTYYL

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfkd2_24e23, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphfkd2_24e23, frame 2

Report for DKFZphfkd2 24e23.2

[LENGTH] 198

[MW] 20948.98

[pi] 6.01

[PROSITE] MYRISTYL 5

[PROSITE] AMIDATION 1

[PROSITE] CAMP PHOSPHO SITE

[PROSITE] CK2 PHOSPHO SITE

[PROSITE] PKC PHOSPHO SITE

[KW] All Beta

[KW] LOW COMPLEXITY 6.06 %

SEQ MADTQCCPPPCEFISSAGTDLALGMGWDATLCLLPFTGFGKCAGIWNHMDEEPDNGDDRG SEG PRD ccccccccccccccccccccccccccccceeeeeccccccceeeeccccccccccccccc

SEQ SRRTTGQGRKWAAHGTMAAPRVHTDYHPGGGSACSSVKVRSHVGHTGVFFFVDQDPLAVS SEG PRD cccccccccccccccccccceeeeecccccccccceeeeeeeccccceeeeeccccceee

SEQ LTSQSLIPPLIKPGLLKAWGFLLLCAQPSANGHSLCCLLYTDLVSSHELSPFRALCLGPS SEG xxxxxxxxxxxx PRD eccccccccccccchhhhhhhhhhhccccccccceeeeeeeeeccccccccceeeecccc

SEQ DAPSACASCNCLASTYYL SEG PRD cccccccccccccccccc

Prosite for DKFZphf d2_24e23.2

PS00004 62->66 CAMP_PHOSPHO_SITE PDOC00004 PS00005 61->64 PKC_PHOSPHO_SITE PDOC00005 PS00005 96->99 PKC_PHOSPHO_SITE PDOC00005 PS00006 165->169 CK2_PHOSPHO_SITE PDOC00006 PS00008 18->24 MYRISTYL PDOC00008 PS00008 60->66 MYRISTYL PDOC00008 PS00008 89->95 MYRISTYL PDOC00008 PS00008 91->97 MYRISTYL PDOC00008 PS00008 134->140 MYRISTYL PDOC00008 PS00009 67->71 AMIDATION PDOC00009

(No Pfam data available for DKFZphfkd2 24e23.2) DKFZphfkd2_24n20

group: intracellular transport and trafficking

DKFZphf d2_24n20.3 encodes a novel 366 amino acid protein with similarity to human epsδ binding protein e3Bl and spectrins .

The new protein contains an Src homology domain 3 and is similar to human eps8 SH3 domain binding protein 1 (e3Bl) and spectrins. Eps8 is a substrate of receptor tyrosine kinases involved in mitogenic signaling. Spectrin is part of the submembrane cytoskeletal network in the human erythrocyte ghost. Nonerythroid spectrins are proposed to have roles in cell adhesion, establishment of cell polarity, and attachment of other cytoskeletal structures to the plasma membrane. The new protein seems to be part of the signalling pathway between tyrosine kinases and the membrane/cyto skeleton.

The new protein can find application in modulating cell adhesion/motility and membrane/cyto skeleton structure and dynamics. strong similarity to eps8 binding protein e3Bl complete cDNA, complete eds, few EST hits potential start at Bp 300, but there are ATGs in other frames in

5 ' region of the cDNA

Sequenced by GBF

Locus: /map="17"

Insert length: 1719 bp

Poly A stretch at pos. 1699, polyadenylation signal at pos. 1680

1 GGGGACAGCT GCCCCGACCT TGGCTTCCTC TGCTGGGTGG GATTGGGGGC

51 TGGGCCCCCA AATGGGCCCC TGGCTTCCCC CTTCCTCTGG GCAGGGGACA

101 GAGAGACACA GGCTCGGGGA GCAGGACTGA CTTCCTCTTG TCCCGGAATG

151 AGCATGCCTG CCCTTTGCAA GCAGGTTTGG GTCTCACGCA GAGGAAACCA

201 AAAGCAATAA GAGGGAGGGA AGGCAGAGCA ACCAATCAAG GGCAGGGTGA

251 GACTCAAAAC GAGCGGGCTC CCTGGGGAGC CAGACAGAGG CTGGGGGTGA

301 TGGCGGAGCT ACAGCAGCTG CAGGAGTTTG AGATCCCCAC TGGCCGGGAG

351 GCTCTGAGGG GCAACCACAG TGCCCTGCTG CGGGTCGCTG ACTACTGCGA

401 GGACAACTAT GTGCAGGCCA CAGACAAGCA GAAGGCGCTG GAGGAGACCA

451 TGGCCTTCAC TACCCAGGCA CTGGCCAGCG TGGCCTACCA GGTGGGCAAC

501 CTGGCCGGGC ACACTCTGCG CATGTTGGAC CTGCAGGGGG CCGCCCTGCG

551 GCAGGTGGAA GCCCGTGTAA GCACGCTGGG CCAGATGGTG AACATGCATA

601 TGGAGAAGGT GGCCCGAAGG GAGATCGGCA CCTTAGCCAC TGTCCAGCGG

651 CTGCCCCCCG GCCAGAAGGT CATCGCCCCA GAGAACCTAC CCCCTCTCAC

701 GCCCTACTGC AGGAGACCCC TCAACTTTGG CTGCCTGGAC GACATTGGCC

751 ATGGGATCAA GGACCTCAGC ACGCAGCTGT CAAGAACAGG CACCCTGTCT

801 CGAAAGAGCA TCAAGGCCCC TGCCACACCC GCCTCCGCCA CCTTGGGGAG

851 ACCGCCCCGG ATTCCCGAGC CAGTGCACCT GCCGGTGGTG CCCGACGGCA

901 GACTCTCCGC CGCCTCCTCT GCGTCTTCCC TGGCCTCGGC CGGCAGCGCC

951 GAAGGTGTCG GTGGGGCCCC CACGCCCAAG GGGCAGGCAG CACCTCCAGC

1001 CCCACCTCTC CCCAGCTCCT TGGACCCACC TCCTCCACCA GCAGCCGTCG

1051 AGGTGTTCCA GCGGCCTCCC ACGCTGGAGG AGTTGTCCCC ACCCCCACCG

1101 GACGAAGAGC TGCCCCTGCC ACTGGACCTG CCTCCTCCTC CACCCCTGGA

1151 TGGAGATGAA TTGGGGCTGC CTCCACCCCC ACCAGGATTT GGGCCTGATG

1201 AGCCCAGCTG GGTGCCTGCC TCATACTTGG AGAAAGTGGT GACACTGTAC

1251 CCATACACCA GCCAGAAGGA CAATGAGCTC TCCTTCTCTG AGGGCACTGT

1301 CATCTGTGTC ACTCGCCGCT ACTCCGATGG CTGGTGCGAG GGCGTCAGCT

1351 CGGAGGGGAC TGGATTCTTC CCTGGGAACT ATGTGGAGCC CAGCTGCTGA

1401 CAGCCCAGGG CTCTCTGGGC AGCTGATGTC TGCACTGAGT GGGTTTCATG

1451 AGCCCCAAGC CAAAACCAGC TCCAGTCACA GCTGGACTGG GTCTGCCCAC

1501 CTCTTGGGCT GTGAGCTGTG TTCTGTCCTT CCTCCCATCG GAGGGAGAAG

1551 GGGTCCTGGG GAGAGAGAAT TTATCCAGAG GCCTGCTGCA GATGGGGAAG

1601 AGCTGGAAAC CAAGAAGTTT GTCAACAGAG GACCCCTACT CCATGCAGGA

1651 CAGGGTCTCC TGCTGCAAGT CCCAACTTTG AATAAAACAG ATGATGTCCA

1701 AAAAAAAAAA AAAAAAAAA

BLAST Results

Entry AC004797 from database EMBL:

Homo sapiens chromosome 17, clone hRPC.62_0_9, complete sequence.

Score = 2316, P = 5.9e-255, identities = 464/465

7 exons Bp 93317-110902 Medline entries

97163405:

Isolation and characterization of e3Bl, an eps8 binding protein that regulates cell growth.

98256293:

Identification of a candidate human spectrin Src homology 3 domain-binding protein suggests a general mechanism of association of tyrosine kinases with the spectrm-based membrane skeleton.

Peptide information for frame 3

ORF from 300 bp to 1397 bp; peptide length: 366 Category: strong similarity to known protein

1 MAELQQLQEF EIPTGREALR GNHSALLRVA DYCEDNYVQA TDKQKALEET 51 MAFTTQALAS VAYQVGNLAG HTLRMLDLQG AALRQVEARV STLGQMVNMH 101 MEKVARREIG TLATVQRLPP GQKVIAPENL PPLTPYCRRP LNFGCLDDIG 151 HGIKDLSTQL SRTGTLSRKS IKAPATPASA TLGRPPRIPE PVHLPVVPDG 201 RLSAASSASS LASAGSAEGV GGAPTPKGQA APPAPPLPSS LDPPPPPAAV 251 EVFQRPPTLE ELSPPPPDEE LPLPLDLPPP PPLDGDELGL PPPPPGFGPD 301 EPSWVPASYL EKVVTLYPYT SQKDNELSFS EGTVICVTRR YSDGWCEGVS 351 SEGTGFFPGN YVEPSC

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphfkd2_24n20, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphf d2_24n20, frame 3

Report for DKFZphfkd2_24n20.3

[LENGTH] 366 [MW] 38947.21 [pi] 4.93 [HOMOL] TREMBL:U87166 1 gene: "SSH3BP1"; product: "spectrm SH3 domain binding protein

Homo sapiens spectrm SH3 domain binding protein 1 (SSH3BP1) mRNA, complete eds. 3e-48

[FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YGR136w] 9e-06

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YGR136w] 9e-06

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YPR154w] 3e-05

[FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YDR388w] 2e-04

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YDR388w]

2e-04

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YDR162c] 4e-04

[BLOCKS] BL50002B Src homology 3 (SH3) domain proteins profile

[SUPFAM] SH3 homology 6e-17

[PROSITE] MYRISTYL 6

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOΞPHO_SITE 6

[PROSITE] PKC_PHOSPHO_SITE 8

[PROSITE] ASN_GLYCOSYLATION 1

[PFAM] Src homology domain 3

[KW] Irregular

[KW] 3D

[KW] LOW COMPLEXITY 24.04 %

SEQ MAELQQLQEFEIPTGREALRGNHSALLRVADYCEDNYVQATDKQKALEETMAFTTQALAS

SEG laboA

SEQ VAYQVGNLAGHTLRMLDLQGAALRQVEARVSTLGQMVNMHMEKVARREIGTLATVQRLPP

SEG laboA SEQ GQKVIAPENLPPLTPYCRRPLNFGCLDDIGHGIKDLSTQLSRTGTLSRKSIKAPATPASA SEG laboA

SEQ TLGRPPRIPEPVHLPVVPDGRLSAASSASSLASAGSAEGVGGAPTPKGQAAPPAPPLPSS SEG xxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxx laboA

SEQ LDPPPPPAAVEVFQRPPTLEELSPPPPDEELPLPLDLPPPPPLDGDELGLPPPPPGFGPD SEG xxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx laboA

SEQ EPSWVPASYLEKVVTLYPYTSQKDNELSFSEGTVICVTRRYSDGWCEGVSSEGTGFFPGN SEG xx laboA EECCCBCCCTTTBCCBTTTEEEEEEEETTTTEEEEEETTEEEEEEGG

SEQ YVEPSC SEG laboA GEEE..

Prosite for DKFZphfkd2_24n20.3

PS00001 22->26 ASN_GLYCOSYLATION PDOC00001 PS00004 339->343 CAMP_PHOSPHO_SITE PDOC00004 PS00005 14->17 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 41->44 PKC_PHOSPHO_SITE PDOC00005 PS00005 72->75 PKC_PHOSPHO_SITE PDOC00005 PS00005 167->170 PKC_PHOSPHO_SITE PDOC00005 PS00005 170->173 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 225->228 PKC_PHOSPHO_SITE PDOC00005 PS00005 321->324 PKC_PHOSPHO_SITE PDOC00005 PS00005 338->341 PKC_PHOSPHO_SITE PDOC00005 PS00006 14->18 CK2_PHOSPHO_SITE PDOC00006 PS00006 239->243 CK2_PHOSPHO_SITE PDOC00006 PS00006 258->262 CK2_PHOSPHO_SITE PDOC00006 PS00006 308->312 CK2_PHOSPHO_SITE PDOC00006 PS00006 321->325 CK2_PHOSPHO_SITE PDOC00006 PS00006 328->332 CK2_PHOSPHO_SITE PDOC00006 PS00008 21->27 MYRISTYL PDOC00008 PS00008 66->72 MYRISTYL PDOC00008 PS00008 94->100 MYRISTYL PDOC00008 PS00008 110->116 MYRISTYL PDOC00008 PS00008 215->221 MYRISTYL PDOC00008 PS00008 332->338 MYRISTYL PDOC00008

Pfam for DKFZphf d2_24n20.3

HMM_NAME Src homology domain 3

HMM *pyVIALYDYqAqdpDELSFkEGDIIιIIEdsDD.WWrgRnnnTNGQEGW

++V+ LY+Y++Q ++ELSF EG +1 + + D W++G + +G+

Query 311 EKVVTLYPYTSQKDNELSFSEGTVICVTRRYSDGWCEGVSSE GTGF 356

HMM IPSNYVEPi* +P NYVEP

Query 357 FPGNYVEPS 365

DKFZphfkd2_24p5

group: intracellular transport and trafficking

DKFZphfkd2_24p5 encodes a novel 811 amino acid protein which is a novel splice variant of human ankyrin G.

The ankyrin 3 gene encodes a novel ankyrin, which is expressed m multiple tissues, with very high expression at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Ankyrin G shows several tissue-specific alternative mRNA processing. The different ankyrin G proteins participate in maintenance/targeting of ion channels and cell adhesion molecules to nodes of Ranvier and axonal initial segments.

The new protein can find application in modulating the structure and membrane topology of Ranvier nodes and other neuronal cell membranes.

Human ankyrin G (ANK-3) new splice variant splice variant potential frame shift at 2720 was checked see BLASTX

Sequenced by EMBL

Locus: /map="10q21"

Insert length: 3470 bp

Poly A stretch at pos. 3459, no polyadenylation signal found

1 AGCTTTAAAA GGATGTCTGC GAAGTGGTCA AAAGGATCTT AACCTCAATT

51 AAGTGGGGTT TTTTAAAAAG ATTTTTTGGG GGGCCTGAAA TTTTGAAAAT

101 CTTCGAACTC TGAGTGGGGA AAGATGTATA ATTCCTCAAT TGCCTACGAG

151 GATATCAAGA TGCTGAGAGG AATTCAGCGG TGGTGAAGAG AGTGGATACA

201 AACCAGGGAT TGGTTTCCTT GAGCTGTTTT GGAGGTTGAT TCTAAATCAC

251 TGCTTAAGGA ATTCCTGGAA ACATCAGGAA AACATTTGAT CATCCAAGCC

301 TAGTGGAAAT GGCTTTACCG CAGAGTGAAG ATGCAATGAC CGGGGACACA

351 GACAAATATC TTGGGCCACA GGACCTTAAG GAATTGGGTG ATGATTCCCT

401 GCCTGCAGAG GGTTACATGG GCTTTAGTCT CGGAGCGCGT TCTGCCAGCC

451 TCCGCTCCTT CAGTTCGGAT GGGTCTTACA CCTTGAACAG AAGCTCCTAT

501 GCACGGGACA GCATGATGAT TGAAGAACTC CTCGTGCCAT CCAAAGAGCA

551 GCATCTAACA TTCACAAGGG AATTTGATTC AGATTCTCTT AGACATTACA

601 GCTGGGCTGC AGACACCTTA GACAATGTCA ATCTTGTTCC AAGCCCCATT

651 CATTCTGGGT TTCTGGTTAG CTTTATGGTG GACGCGAGAG GGGGCTCCAT

701 GAGAGGAAGC CGTCATCACG GGATGAGAAT CATCATTCCT CCACGCAAGT

751 GTACGGCCCC CACTCGAATC ACCTGCCGTT TGGTAAAGAG ACATAAACTG

801 GCCAACCCAC CCCCCATGGT GGAAGGAGAG GGATTAGCCA GTAGGCTGGT

851 AGAAATGGGT CCTGCAGGGG CACAATTTTT AGGCCCTGTC ATAGTGGAAA

901 TCCCTCACTT TGGGTCCATG AGAGGAAAAG AGAGAGAACT CATTGTTCTT

951 CGAAGTGAAA ATGGTGAAAC TTGGAAGGAG CATCAGTTTG ACAGCAAAAA

1001 TGAAGATTTA ACCGAGTTAC TTAATGGCAT GGATGAAGAA CTTGATAGCC

1051 CAGAAGAGTT AGGGAAAAAG CGTATCTGCA GGATTATCAC GAAAGATTTC

1101 CCCCAGTATT TTGCAGTGGT TTCCCGGATT AAGCAGGAAA GCAACCAGAT

1151 TGGTCCTGAA GGTGGAATTC TGAGCAGCAC CACAGTGCCC CTTGTTCAAG

1201 CATCTTTCCC AGAGGGTGCC CTAACTAAAA GAATTCGAGT GGGCCTCCAG

1251 GCCCAGCCTG TTCCAGATGA AATTGTGAAA AAGATCCTTG GAAACAAAGC

1301 AACTTTTAGC CCAATTGTCA CTGTGGAACC AAGAAGACGG AAATTCCATA

1351 AACCAATCAC AATGACCATT CCGGTGCCCC CGCCCTCAGG AGAAGGTGTA

1401 TCCAATGGAT ACAAAGGGGA CACTACACCC AATCTGCGTC TTCTCTGTAG

1451 CATTACAGGG GGCACTTCGC CTGCTCAGTG GGAAGACATC ACAGGAACAA

1501 CTCCTTTGAC GTTTATAAAA GATTGTGTCT CCTTTACAAC CAATGTTTCA

1551 GCCAGATTTT GGCTTGCAGA CTGCCATCAA GTTTTAGAAA CTGTGGGGTT

1601 AGCCACGCAA CTGTACAGAG AATTGATATG TGTTCCATAT ATGGCCAAGT

1651 TTGTTGTTTT TGCCAAAATG AATGATCCCG TAGAATCTTC CTTGCGATGT

1701 TTCTGCATGA CAGATGACAA AGTGGACAAA ACTTTAGAGC AACAAGAGAA

1751 TTTTGAGGAA GTCGCAAGAA GCAAAGATAT TGAGGTTCTG GAAGGAAAAC

1801 CTATTTATGT TGATTGTTAT GGAAATTTGG CCCCACTTAC CAAAGGAGGA

1851 CAGCAACTTG TTTTTAACTT TTATTCTTTC AAAGAAAATA GACTGCCATT

1901 TTCCATCAAG ATTAGAGACA CCAGCCAAGA GCCCTGTGGT CGTCTGTCTT

1951 TTCTGAAAGA ACCAAAGACA ACAAAAGGAC TGCCTCAAAC AGCGGTTTGC

2001 AACTTAAATA TCACTCTGCC AGCACATAAA AAGATTGAGA AAACAGATGG

2051 ACGACAGAGC TTCGCATCCT TAGCTTTACG TAAGCGCTAC AGCTACTTGA

2101 CTGAGCCTGG AATGAGTCCA CAGAGTCCAT GTGAACGGAC AGATATCAGG

2151 ATGGCAATAG TAGCCGATCA CCTGGGACTT AGTTGGACAG AACTGGCAAG

2201 GGAACTGAAT TTTTCAGTGG ATGAAATCAA TCAAATACGT GTGGAAAATC

2251 CAAATTCTTT AATTTCTCAG AGCTTCATGT TTTTAAAAAA ATGGGTTACC

2301 AGAGACGGAA AAAATGCCAC AACTGATGCC TTAACTTCGG TCTTGACAAA

2351 AATTAATCGA ATAGATATAG TGACACTGCT AGAAGGACCA ATATTTGATT 2401 ATGGAAATAT TTCAGGCACC AGAAGTTTTG CAGATGAGAA CAATGTTTTC

2451 CATGACCCTG TTGATGGTTA TCCTTCCCTT CAAGTGGAAC TGGAAACCCC

2501 CACAGGGTTG CACTACACAC CACCTACCCC TTTCCAGCAA GATGATTATT

2551 TTAGTGATAT CTCTAGCATA GAATCTCCCC TTAGAACCCC TAGTAGACTG

2601 AGTGATGGGC TAGTGCCTTC CCAGGGGAAC ATAGAGCATT CCGCAGATGG

2651 ACCTCCAGTC GTAACTGCAG AAGACGCTTC CTTAGAAGAC AGCAAACTGG

2701 AAGACTCAGT GCCTTTAACA GAAATGCCTG AAGCAGTGAT GTAGATGAGA

2751 GCCAGTTGGA GAATGTATGT CTGAGTTGGC AGAATGAGAC ATCAAGTGGA

2801 AACCTAGAGT CCTGCGCTCA AGCTCGAAGA GTAACTGGTG GGTTACTAGA

2851 TCGACTGGAT GACAGCCCTG ACCAGTGTAG AGATTCCATT ACCTCATATC

2901 TCAAAGGAGA AGCTGGCAAA TTTGAAGCAA ATGGAAGCCA TACAGAAATC

2951 ACTCCAGAAG CAAAGACAAA ATCTTACTTT CCAGAATCCC AAAATGATGT

3001 AGGAAAACAG AGTACCAAGG AAACTCTGAA ACCAAAAATA CATGGATCTG

3051 GTCATGTTGA AGAACCAGCA TCACCACTAG CAGCATATCA GAAATCTCTA

3101 GAAGAAACCA GCAAGCTTAT AATAGAAGAG ACTAAACCCT GTGTGCCTGT

3151 CAGTATGAAA AAGATGAGTA GGACTTCTCC AGCAGATGGC AAGCCAAGGC

3201 TTAGCCTCCA TGAAGAAGAG GGGTCCAGTG GGTCTGAGCA AAAGCAGGGA

3251 GAAGGTTTTA AGGTGAAAAC GAAGAAAGAA ATCCGGCATG TGGAAAAGAA

3301 GAGCCACTCG TAACAGCGAA CGGTCAGTCA AGGATCATAA GTTTTTACTG

3351 CCAGTATTGA GAAATTCGTG GAAGAAATGT CAGCAGGAAG TAAAAATTCA

3401 CCGAGAAGTG TGTGTGTGTT CGCTGCTTCC ACACATTAAT GGCATGATTT

3451 TTTTTATGCA AAAAAAAAAA

BLAST Results

Entry MMANK3A_1 from database TREMBL: Ank3"; product: "ankyrin 3"; Mus mu. +3 4022 0.0

Entry HS13616 from database EMBL:

Human ankyrin G (ANK-3) mRNA, complete eds.

Length = 14,770

Plus Strand HSPs:

Score = 8505 (1276.1 bits), Expect = 0.0, Sum P 3) = 0.0

Identities = 1799/1873 (96%)

Medline entries

95394457:

Chromosomal localization of the ankyπnG gene

(ANK3/Ank3) to human 10q21 and mouse 10.

95138209:

A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of Ranvier

Peptide information for frame 3

ORF from 309 bp to 2741 bp; peptide length: 811 Category: known protein Classification: unset

1 MALPQSEDAM TGDTDKYLGP QDLKELGDDS LPAEGYMGFS LGARSASLRS 51 FSSDGSYTLN RSSYARDSMM IEELLVPSKE QHLTFTREFD SDSLRHYSWA 101 ADTLDNVNLV PSPIHSGFLV SFMVDARGGS MRGSRHHGMR IIIPPRKCTA 151 PTRITCRLVK RHKLANPPPM VEGEGLASRL VEMGPAGAQF LGPVIVEIPH 201 FGSMRGKERE LIVLRSENGE TWKEHQFDSK NEDLTELLNG MDEELDSPEE 251 LGKKRICRII TKDFPQYFAV VSRIKQESNQ IGPEGGILSS TTVPLVQASF 301 PEGALTKRIR VGLQAQPVPD EIVKKILGNK ATFSPIVTVE PRRRKFHKPI 351 TMTIPVPPPS GEGVSNGYKG DTTPNLRLLC SITGGTSPAQ WEDITGTTPL 401 TFIKDCVSFT TNVSARFWLA DCHQVLETVG LATQLYRELI CVPYMAKFVV 451 FAKMNDPVES SLRCFCMTDD KVDKTLEQQE NFEEVARSKD IEVLEGKPIY 501 VDCYGNLAPL TKGGQQLVFN FYSFKENRLP FSIKIRDTSQ EPCGRLSFLK 551 EPKTTKGLPQ TAVCNLNITL PAHKKIEKTD GRQSFASLAL RKRYSYLTEP 601 GMSPQSPCER TDIRMAIVAD HLGLSWTELA RELNFSVDEI NQIRVENPNS 651 LISQSFMFLK KWVTRDGKNA TTDALTSVLT KINRIDIVTL LEGPIFDYGN 701 ISGTRΞFADE NNVFHDPVDG YPSLQVELET PTGLHYTPPT PFQQDDYFSD 751 ISSIESPLRT PSRLSDGLVP SQGNIEHSAD GPPVVTAEDA SLEDSKLEDS 801 VPLTEMPEAV M

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphfkd2_24p5, frame 3

TREMBL:MMANK3A_1 gene: "Ank3"; product: "ankyrin 3"; Mus musculus epithelial ankyrin 3 (Ank3) 5kb isoform mRNA, complete eds., N = 1, Score = 4022, P = 0

TREMBL :MMANK3B_3 gene: "Ank3"; product: "ankyrin 3"; Mus musculus epithelial ankyrin 3 (7kb isoform) mRNA, complete eds., N = 1, Score = 4005, P = 0

TREMBL :MMANK3B_4 gene: "Ank3"; product: "ankyrin 3"; Mus musculus epithelial ankyrin 3 (7kb isoform) mRNA, complete eds., N = 1, Score = 4005, P = 0

>TREMBL : MMANK3A_1 gene: "Ank3"; product: "ankyrin 3"; Mus musculus epithelial ankyrin 3 (Ank3) 5kb isoform mRNA, complete eds. Length = 1,094

HSPs:

Score = 4022 (603.5 bits), Expect = O.Oe+00, P = O.Oe+00 Identities = 769/805 (95%), Positives = 783/805 (97%)

Query: 1 MALPQSEDAMTGDTDKYLGPQDLKELGDDSLPAEGYMGFSLGARSASLRSFSSDGSYTLN 60

MALP SEDA+TGDTDKYLGPQDLKELGDDSLPAEGY+GFSLGARSASLRSFSSD SYTLN Sbjct: 1 MALPHSEDAITGDTDKYLGPQDLKELGDDSLPAEGYVGFSLGARSASLRΞFSSDRSYTLN 60

Query: 61 RSSYARDSMMIEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLVPSPIHSGFLV 120

RSSYARDSMMIEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLV SP+HSGFLV Sbjct: 61 RSSYARDSMMIEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLVΞSPVHSGFLV 120

Query: 121 SFMVDARGGSMRGSRHHGMRIIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRL 180

SFMVDARGGSMRGSRHHGMRIIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRL Sbjct: 121 SFMVDARGGΞMRGSRHHGMRIIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRL 180

Query: 181 VEMGPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGETWKEHQFDSKNEDLTELLNG 240

VEMGPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGETWKEHQFDSKNEDL ELLNG Sbjct: 181 VEMGPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGETWKEHQFDSKNEDLAELLNG 240

Query: 241 MDEELDSPEELGKKRICRIITKDFPQYFAVVSRIKQESNQIGPEGGILSSTTVPLVQASF 300

MDEELDSPEELG KRICRIITKDFPQYFAVVSRIKQESNQIGPEGGILSSTTVPLVQASF Sbjct: 241 MDEELDSPEELGTKRICRIITKDFPQYFAVVSRIKQESNQIGPEGGILSSTTVPLVQASF 300

Query: 301 PEGALTKRIRVGLQAQPVPDEIVKKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPS 360

PEGALTKRIRVGLQAQPVP+E VKKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPS Sbjct: 301 PEGALTKRIRVGLQAQPVPEETVKKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPS 360

Query: 361 GEGVSNGYKGDTTPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLA 420

GEGVSNGYKGD TPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLA Sbjct: 361 GEGVΞNGYKGDATPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLA 420

Query: 421 DCHQVLETVGLATQLYRELICVPYMAKFVVFAKMNDPVESSLRCFCMTDDKVDKTLEQQE 480

DCHQVLETVGLA+QLYRELICVPYMAKFVVFAK NDPVESSLRCFCMTDD+VDKTLEQQE Sbjct: 421 DCHQVLETVGLASQLYRELICVPYMAKFVVFAKTNDPVESSLRCFCMTDDRVDKTLEQQE 480

Query: 481 NFEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQ 540

NFEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQ Sbjct: 481 NFEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQ 540

Query: 541 EPCGRLΞFLKEPKTTKGLPQTAVCNLNITLPAHKKIEKTDGRQSFASLALRKRYSYLTEP 600

EPCGRLSFLKEPKTTKGLPQTAVCNLNITLPAHKK EK D RQSFASLALRKRYSYLTEP Sbjct: 541 EPCGRLSFLKEPKTTKGLPQTAVCNLNITLPAHKKAEKADRRQSFASLALRKRYSYLTEP 600

Query: 601 GMSPQSPCERTDIRMAIVADHLGLSWTELARELNFSVDEINQIRVENPNSLISQSFMFLK 660

MSPQSPCERTDIRMAIVADHLGLSWTELARELNFSVDEINQIRVENPNSLISQSFM LK Sbjct: 601 SMSPQSPCERTDIRMAIVADHLGLSWTELARELNFSVDEINQIRVENPNSLISQSFMLLK 660

Query: 661 KWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGNISGTRSFADENNVFHDPVDG 720

KWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGNISGTRSFADENNVFHDPVDG Sbjct: 661 KWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGNISGTRSFADENNVFHDPVDG 720

Query: 721 YPSLQVELETPTGLHYTPPTPFQQDDYFSDISSIESPLRTPSRLSDGLVPSQGNIEHSAD 780

+PS QVELETP GL++TPP PFQQDD+FSDISSIESP RTPSRLSDGLVPSQGNIEH Sbjct: 721 HPSFQVELETPMGLYWTPPNPFQQDDHFSDISSIESPFRTPSRLSDGLVPSQGNIEHPTG 780

Query: 781 GPPVVTAEDASLEDSKLEDSVPLTE 805 GPPVVTAED SLEDSK++DSV +T+ Sbjct: 781 GPPWTAEDTSLEDSKMDDSVTVTD 805

Pedant information for DKFZphfkd2_24p5, frame 3

Report for DKFZphfkd2_24p5.3

[LENGTH] 811

[MW] 90104.66

[pi] 5.40

[HOMOL] TREMBL :MMANK3A_1 gene: "Ank3"; product: "ankyrin 3"; Mus musculus epithelial ankyrin 3 (Ank3) 5kb isoform mRNA, complete eds. 0.0

[BLOCKS] BL50017B Death domain proteins profile

[PIRKW] phosphoprotein 0.0

[PIRKW] alternative splicing 0.0

[PIRKW] peripheral membrane protein 0.0

[PIRKW] cytoskeleton 0.0

[SUPFAM] ankyrin 0.0

[SUPFAM] ankyrin repeat homology 0.0

[SUPFAM] unassigned ankyrin repeat proteins 0.0

[KW] TRANSMEMBRANE 2

[KW] LOW COMPLEXITY 1.73 %

SEQ MALPQSEDAMTGDTDKYLGPQDLKELGDDSLPAEGYMGFSLGARSASLRSFSSDGSYTLN

SEG

PRD cccccccccccccccccccccccccccccccccccccccccccccceeeeeccccccccc

MEM

SEQ RSSYARDSMMIEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLVPSPIHSGFLV

SEG

PRD cccchhhhhhhhheeeehhhhhhhhhhhccccccccccccccccccccccccccccceee

MEM MMMMMMMMMMMM

SEQ ΞFMVDARGGSMRGSRHHGMRIIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRL

SEG xxxxxxxxxxxxxx

PRD eeeeeccccccccccccceeeecccccccccceeeeehhhhhccccccccccccccccee

MEM MMMMMMMMMMMMMMMM M

SEQ VEMGPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGETWKEHQFDSKNEDLTELLNG

SEG

PRD eecccccceeeceeeeeeccccccccccceeeeeeccccceeeeeccccccchhhhhhhc

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ MDEELDSPEELGKKRICRIITKDFPQYFAVVSRIKQESNQIGPEGGILSSTTVPLVQASF

SEG

PRD cccccchhhhhhhhheeeeeeccccceeeeehhhhhcccccccccccccceeeeeeeccc

MEM

SEQ PEGALTKRIRVGLQAQPVPDEIVKKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPS

SEG

PRD ccchhhhhhhhhhhhhccccceeeeccccccccccceeeccccccccccceeeecccccc

MEM

SEQ GEGVSNGYKGDTTPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLA

SEG

PRD ccccccccccccccceeeeeeeeccccccccccccccceeeeeeccccccccccceeeec

MEM

SEQ DCHQVLETVGLATQLYRELICVPYMAKFVVFAKMNDPVESSLRCFCMTDDKVDKTLEQQE

SEG

PRD cchhhhhhhhhhhhhhhhhhhhcchhhhhheeecccchhhhhhhhccccchhhhhhhhhc

MEM

SEQ NFEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQ

SEG

PRD cceeecccceeeeeeccceeeeecccccccchhhhhhhhhchhhhhhhcceeeeeecccc

MEM

SEQ EPCGRLSFLKEPKTTKGLPQTAVCNLNITLPAHKKIEKTDGRQSFASLALRKRYSYLTEP

SEG '

PRD ccccceeeeccccccccccccccccccccccccccccccccchhhhhhhhhhhhheeecc

MEM

SEQ GMSPQΞPCERTDIRMAIVADHLGLSWTELARELNFSVDEINQIRVENPNSLISQSFMFLK

SEG

PRD ccccccchhhhhhhhhhhhhhhccchhhhhhhhhhhhhhcceeeeecccchhhhhhhhhh

MEM SEQ KWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGNISGTRSFADENNVFHDPVDG

SEG

PRD hhhhcccccccchhhhhhhhhhcceeeeeeeccccccccccccccccccccccccccccc

MEM

SEQ YPSLQVELETPTGLHYTPPTPFQQDDYFSDISΞIESPLRTPSRLSDGLVPSQGNIEHSAD

SEG

PRD cccceeeeeccccccccccccccccccccceeeccccccccccccccccccccccccccc

MEM

SEQ GPPVVTAEDASLEDSKLEDSVPLTEMPEAVM

SEG

PRD ccceeeecccccccccccccccccccccccc

MEM

(No Prosite data available for DKFZphfkd2_24p5.3) (No Pfam data available for DKFZphfkd2_24p5.3)

DKFZphfkd2_3ιl3

group: transmembrane protein

DKFZphfkd2_3ιl3 encodes a novel 406 ammo acid protein with C. elegans cosmid Y37D8A and A. thaliana H71412 hypothetical protein.

The novel protein contains 3 transmembrane regions.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of kidney-specific genes and as a new marker for kidney cells. similarity to A. thaliana and C. elegans; membrane regions : 3 complete cDNA, complete eds, EST hits

Sequenced by BMFZ

Locus: /map="17"

Insert length: 2052 bp

Poly A stretch at pos. 2032, no polyadenylation signal found

1 AGTGACGTGA GCGGGTTCCG GTTGTCTGGA GCCCAGCGGC GGGTGTGAGA 51 GTCCGTAAGG AGCAGCTTCC AGGATCCTGA GATCCGGAGC AGCCGGGGTC

101 GGAGCGGCTC CTCAAGAGTT ACTGATCTAT GAAATGGCAG AGAATGGAAA

151 AAATTGTGAC CAGAGACGTG TAGCAATGAA CAAGGAACAT CATAATGGAA

201 ATTTCACAGA CCCCTCTTCA GTGAATGAAA AGAAGAGGAG GGAGCGGGAA

251 GAAAGGCAGA ATATTGTCCT GTGGAGACAG CCGCTCATTA CCTTGCAGTA

301 TTTTTCTCTG GAAATCCTTG TAATCTTGAA GGAATGGACC TCAAAATTAT

351 GGCATCGTCA AAGCATTGTG GTGTCTTTTT TACTGCTGCT TGCTGTGCTT

401 ATAGCTACGT ATTATGTTGA AGGAGTGCAT CAACAGTATG TGCAACGTAT

451 AGAGAAACAG TTTCTTTTGT ATGCCTACTG GATAGGCTTA GGAATTTTGT

501 CTTCTGTTGG GCTTGGAACA GGGCTGCACA CCTTTCTGCT TTATCTGGGT

551 CCACATATAG CCTCAGTTAC ATTAGCTGCT TATGAATGCA ATTCAGTTAA

601 TTTTCCCGAA CCACCCTATC CTGATCAGAT TATTTGTCCA GATGAAGAGG

651 GCACTGAAGG AACCATTTTT TTGTGGAGTA TCATCTCAAA AGTTAGGATT

701 GAAGCCTGCA TGTGGGGTAT CGGTACAGCA ATCGGAGAGC TGCCTCCATA

751 TTTCATGGCC AGAGCAGCTC GCCTCTCAGG TGCTGAACCA GATGATGAAG

801 AGTATCAGGA ATTTGAAGAG ATGCTGGAAC ATGCAGAGTC TGCACAAGAC

851 TTTGCCTCCC GGGCCAAACT GGCAGTTCAA AAACTAGTAC AGAAAGTTGG

901 ATTTTTTGGA ATTTTGGCCT GTGCTTCAAT TCCAAATCCT TTATTTGATC

951 TGGCTGGAAT AACGTGTGGA CACTTTCTGG TACCTTTTTG GACCTTCTTT 1001 GGTGCAACCC TAATTGGAAA AGCAATAATA AAAATGCATA TCCAGAAAAT 1051 TTTTGTTATA ATAACATTCA GCAAGCACAT AGTGGAGCAA ATGGTGGCTT 1101 TCATTGGTGC TGTCCCCGGC ATAGGTCCAT CTCTGCAGAA GCCATTTCAG 1151 GAGTACCTGG AGGCTCAACG GCAGAAGCTT CACCACAAAA GCGAAATGGG 1201 CACACCACAG GGAGAAAACT GGTTGTCCTG GATGTTTGAA AAGTTGGTCG 1251 TTGTCATGGT GTGTTACTTC ATCCTATCTA TCATTAACTC CATGGCACAA 1301 AGTTATGCCA AACGAATCCA GCAGCGGTTG AACTCAGAGG AGAAAACTAA 1351 ATAAGTAGAG AAAGTTTTAA ACTGCAGAAA TTGGAGTGGA TGGGTTCTGC 1401 CTTAAATTGG GAGGACTCCA AGCCGGGAAG GAAAATTCCC TTTTCCAACC 1451 TGTATCAATT TTTACAACTT TTTTCCTGAA AGCAGTTTAG TCCATACTTT 1501 GCACTGACAT ACTTTTTCCT TCTGTGCTAA GGTAAGGTAT CCACCCTCGA 1551 TGCAATCCAC CTTGTGTTTT CTTAGGGTGG AATGTGATGT TCAGCAGCAA 1601 ACTTGCAACA GACTGGCCTT CTGTTTGTTA CTTTCAAAAG GCCCACATGA 1651 TACAATTAGA GAATTCCCAC CGCACAAAAA AAGTTCCTAA GTATGTTAAA 1701 TATGTCAAGC TTTTTAGGCT TGTCACAAAT GATTGCTTTG TTTTCCTAAG 1751 TCATCAAAAT GTATATAAAT TATCTAGATT GGATAACAGT CTTGCATGTT 1801 TATCATGTTA CAATTTAATA TTCCATCCTG CCCAACCCTT CCTCTCCCAT 1851 CCTCAAAAAA GGGCCATTTT ATGATGCATT GCACACCCTC TGGGGAAATT 1901 GATCTTTAAA TTTTGAGACA GTATAAGGAA AATCTGGTTG GTGTCTTACA 1951 AGTGAGCTGA CACCATTTTT TATTCTGTGT ATTTAGGATG AAGTCTTGAA 2001 AAAAACTTTA TAAAGACATC TTTAATCATT CCAAAAAAAA AAAAAAAAAA 2051 AA

BLAST Results

Entry AC004686 from database EMBL:

*** SEQUENCING IN PROGRESS *** Homo sapiens chromosome 17, clone hRPC.1073_F_15; HTGS phase 1, 8 unordered pieces.

Score = 4142, P = 6.1e-199, identities = 830/832 Medline entries

^"^ No Medline entry

Peptide information for frame 2

ORF from 134 bp to 1351 bp; peptide length: 406 Category: similarity to unknown protein

1 MAENGKNCDQ RRVAMNKEHH NGNFTDPSSV NEKKRREREE RQNIVLWRQP

51 LITLQYFSLE ILVILKEWTS KLWHRQSIVV SFLLLLAVLI ATYYVEGVHQ

101 QYVQRIEKQF LLYAYWIGLG ILSSVGLGTG LHTFLLYLGP HIASVTLAAY

151 ECNSVNFPEP PYPDQIICPD EEGTEGTIFL WSIISKVRIE ACMWGIGTAI

201 GELPPYFMAR AARLSGAEPD DEEYQEFEEM LEHAESAQDF ASRAKLAVQK

251 LVQKVGFFGI LACASIPNPL FDLAGITCGH FLVPFWTFFG ATLIGKAIIK

301 MHIQKIFVII TFSKHIVEQM VAFIGAVPGI GPSLQKPFQE YLEAQRQKLH

351 HKSEMGTPQG ENWLSWMFEK LVVVMVCYFI LSIINSMAQS YAKRIQQRLN

401 SEEKTK

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfkd2_3ιl3, frame 2

TREMBL :CEY37D8A_20 gene: "Y37D8A.22"; Caenorhabditis elegans cosmid Y37D8A, N = 1, Score = 905, P = 8.8e-91

TREMBL:ATAC98_2 gene: "YUP8H12.2"; Arabidopsis thaliana chromosome 1 YAC yUP8H12 complete sequence., N = 1, Score = 470, P = l.le-44

PIR:H71412 hypothetical protein - Arabidopsis thaliana, N = 1, Score = 293, P = 6e-24

>TREMBL:CEY37D8A_20 gene: "Y37D8A.22"; Caenorhabditis elegans cosmid Y37D8A

Length = 457

HSPs:

Score = 905 (135.8 bits), Expect = 8.8e-91, P = 8.8e-91 Identities = 167/317 (52%), Positives = 228/317 (71%)

Query: 38 REERQNIVLWRQPLITLQYFSLEILVILKEWTSKLWHRQSIVVSFLLLLAVLIATYYVEG 97

R ER+ IV WR+P I + Y +EI + E K+ +++++ + + + + Y+ G Sbjct: 93 RMERETIVFWRRPHIVIPYALMEIAHLAVELFFKILAHKTVLLLTAISIGLAVYGYHAPG 152

Query: 98 VHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSVNF 157

HQ++VQ IEK L +++W+ LG+LSS+GLG+GLHTFL+YLGPHIA+VT+AAYEC S++F Sbjct: 153 AHQEHVQTIEKHILWWSWWVLLGVLSSIGLGSGLHTFLIYLGPHIAAVTMAAYECQSLDF 212

Query: 158 PEPPYPDQIICPDEEGTEGTIFLWSIISKVRIEACMWGIGTAIGELPPYFMARAARLSGA 217

P+PPYP+ I CP + + F W I++KVR+E+ +WG GTA+GELPPYFMARAAR+SG Sbjct: 213 PQPPYPESIQCPSTKSSIAVTF-WQIVAKVRVESLLWGAGTALGELPPYFMARAARIΞGQ 271

Query: 218 EPDDEEYQEFEEMLE-HAESAQD FASRAKLAVQKLVQKVGFFGILACASIPNPLFD 272

EPDDEEY+EF E++ ES D RAK V+ + ++GF GIL ASIPNPLFD Sbjct: 272 EPDDEEYREFLELMNADKESDADQKLSIVERAKSWVEHNIHRLGFPGILLFASIPNPLFD 331

Query: 273 LAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIITFSKHIVEQMVAFIGAVPGIGP 332

LAGITCGHFLVPFW+FFGATLIGKA++KMH+Q FVI+ FS H E V + +P +GP Sbjct: 332 LAGITCGHFLVPFWSFFGATLIGKALVKMHVQMGFVILAFSDHHAENFVKILEKIPAVGP 391

Query: 333 SLQKPFQEYLEAQRQKLH 350

+++P + LE QR+ LH Sbjct: 392 YIRQPISDLLEKQRKALH 409

Pedant information for DKFZphfkd2_3ιl3, frame 2

Report for DKFZphf d2_3ιl3.2 [LENGTH] 406

[MW] 46298.17

[pi] 6.47

[HOMOL] TREMBL :CEY37D8A_20 gene: "Y37D8A.22"; Caenorhabditis elegans cosmid Y37D8A le-

79

[PROSITE] MYRISTYL 10

[PROSITE] CK2_PHOSPHO_SITE 3

[PROSITE] PKC_PHOSPHO_SITE 1

[PROSITE] ASNJΞLYCOSYLATION 1

[KW] TRANSMEMBRANE 3

[KW] LOW COMPLEXITY 9.85 %

SEQ MAENGKNCDQRRVAMNKEHHNGNFTDPSSVNEKKRREREERQNIVLWRQPLITLQYFSLE

SEG xxxxxxxxxx

PRD ccccccchhhhhhhhhhhccccccccccccchhhhhhhhhhhhhhhhccccchhhhhhhh

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ ILVILKEWTSKLWHRQSIVVSFLLLLAVLIATYYVEGVHQQYVQRIEKQFLLYAYWIGLG

SEG xxxx

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhheeeccchhhhhhhhhhhhhhhhhhhhhh

MEM MM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ ILSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSVNFPEPPYPDQIICPDEEGTEGTIFL SEG xxxxxxxxxxx PRD hccccccccceeeeeeeccchhhhhhhhhhhccccccccccccccccccccccccceeee MEM

SEQ WSIISKVRIEACMWGIGTAIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAESAQDF

SEG xxxxxxxxxxxxxxx PRD eehhhhhhhhhhhhhccccccccccchhhhhhhhcccccchhhhhhhhhhhhhhhhhhhh MEM

SEQ ASRAKLAVQKLVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIK SEG PRD hhhhhhhhhhhhhhhcceeeeeeeecccccccccccccccceeeeeeehhhhhhhhhhhh

MEM MMMMMMMMMMMMMMMMMMMMM

SEQ MHIQKIFVIITFSKHIVEQMVAFIGAVPGIGPSLQKPFQEYLEAQRQKLHHKSEMGTPQG SEG PRD hhhhheeeeeeechhhhhhhhhhhhccccccccchhhhhhhhhhhhhhhhhhcccccccc MEM

SEQ ENWLSWMFEKLVVVMVCYFILSIINSMAQSYAKRIQQRLNSEEKTK SEG PRD cchhhhhhhhhheeehhhhhhhhhhhhhhhhhhhhhhhhhhhcccc MEM

Prosite for DKFZphfkd2_3ιl3.2

PS00001 23->27 ASN_GLYCOSYLATION PDOC00001 PS00005 69->72 PKC_PHOSPHO_SITE PDOC00005 PS00006 29->33 CK2_PHOSPHO_SITE PDOC00006 PS00006 215->219 CK2_PHOSPHO_SITE PDOC00006 PS00006 236->240 CK2_PHOSPHO_SITE PDOC00006 PS00008 120->126 MYRISTYL PDOC00008 PS00008 126-M32 MYRISTYL PDOC00008 PS00008 173->179 MYRISTYL PDOC00008 PS00008 195->201 MYRISTYL PDOC00008 PS00008 197->203 MYRISTYL PDOC00008 PS00008 259->265 MYRISTYL PDOC00008 PS00008 275->281 MYRISTYL PDOC00008 PS00008 325->331 MYRISTYL PDOC00008 PS00008 329->335 MYRISTYL PDOC00008 PS00008 356->362 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfkd2_3ιl3.2) DKFZphfkd2_3ol7

group: metabolism

DKFZphfkd2_3ol7 encodes a novel 72 amino acid protein with similarity to bos taurus NADH- ubiquinone oxidoreductase B33 subunit (EC 1.6.5.3) (EC 1.6.99.3).

NADH:ubιqumone oxidoreductase is the first enzyme in the respiratory electron transport chain of mitochondria. It is a a membrane-bound multi-subunit protein. The bovine heart enzyme contains about 40 different polypeptides. The novel protein is the human orthologue of bovme B22.

The new protein can find application in modulation of the respiratory electron transport chain pathways of mitochondria. strong similarity to bovme NADH-UBIQUINONE OXIDOREDUCTASE B22 subunit complete cDNA, complete eds, EST hits, in frame stop codon at -274 will be checked

ESTs HS1291620/AA883920 show no stop codon at this side

Sequenced by BMFZ

Locus: unknown

Insert length: 693 bp

Poly A stretch at pos. 670, polyadenylation signal at pos. 659

1 CAGCAGGCGT GCAGTTTCCC GGCTCTCCGC GCGGCCGGGG AAGGTCAGCG

51 CCGTAATGGC GTTCTTGGCG TCGGGACCCT ACCTGACCCA TCAGCAAAAG

101 GTGTTGCGGC TTTATAAGCG GGCGCTACGC CACCTCGAGT CGTGGTGCGT

151 CCAGAGAGAC AAATACCGAT ACTTTGCTTG TTTGATGAGA GCCCGGTTTG

201 AAGAACATAA GAATGAAAAG GATATGGCGA AGGCCACCCA GCTGCTGAAG

251 GAGGCCGAGG AAGAATTCTG GTAACGTCAG CATCCACAGC CATACATCTT

301 CCCTGACTCT CCTGGGGGCA CCTCCTATGA GAGATACGAT TGCTACAAGG

351 TCCCAGAATG GTGCTTAGAT GACTGGCATC CTTCTGAGAA GGCAATGTAT

401 CCTGATTACT TTGCCAAGAG AGAACAGTGG AAGAAACTGC GGAGGGAAAG

451 CTGGGAACGA GAGGTTAAGC AGCTGCAGGA GGAAACGCCA CCTGGTGGTC

501 CTTTAACTGA AGCTTTGCCC CCTGCCCGAA AGGAAGGTGA TTTGCCCCCA

551 CTGTGGTGGT ATATTGTGAC CAGACCCCGG GAGCGGCCCA TGTAGAAAGA

601 GAGAGACCTC ATCTTTCATG CTTGCAAGTG AAATATGTTA CAGAACATGC

651 ACTTGCCCTA ATAAAAAATC AGTAAAAAAA AAAAAAAAAA AAA

BLAST Results

Entry S28256 from database PIR:

NADH dehydrogenase (ubiquinone) (EC 1.6.5.3) chain CI-B22 - bovine >TREMBL:MIBTCIB22_1 gene: "CI-B22"; product: "NADH-ubiquinone oxidoreductase complex B22 subunit"; B. taurus mitochondrion cI-B22 mRNA for B22 subunit of the NADH-ubiqumone oxidoreductase complex Score = 933, P = 5.2e-93, identities = 163/179, positives = 172/179, frame +2

Medline entries

92389317

Sequences of 20 subunits of NADH: ubiquinone oxidoreductase from RT bovme heart mitochondria.

Application of a novel strategy for RT sequencing proteins using the polymerase chain reaction

Peptide information for frame 2

ORF from 56 bp to 271 bp; peptide length: 72 Category: strong similarity to known protein

1 MAFLASGPYL THQQKVLRLY KRALRHLESW CVQRDKYRYF ACLMRARFEE

51 HKNEKDMAKA TQLLKEAEEE FW*RQHPQPY IFPDSPGGTS YERYDCYKVP

101 EWCLDDWHPS EKAMYPDYFA KREQWKKLRR ESWEREVKQL QEETPPGGPL

151 TEALPPARKE GDLPPLWWYI VTRPRERPM BLASTP hits

Sequences producing significant alignments: (bits) Value sp|Q02369|NI2M_BOVIN|0D36CE17281FB735 (NDUFB9.. ) NADH-UBIQUINONE... 141 7e-34 tr|U41534 | Q18036 I D34BCCB6E8FBCD5F (C16A3.4) SIMILAR TO NADH-UBIQ... 53 3e-07

>sp|Q02369|NI2M_BOVIN|0D36CE17281FB735 (NDUFB9.. ) NADH-UBIQUINONE OXIDOREDUCTASE B22 SUBUNIT (EC 1.6.5.3) (EC 1.6.99.3) (COMPLEX I-B22) (CI-B22) . [BOS TAURUS] Length = 178

Score = 141 bits (351), Expect = 7e-34 Identities = 63/71 (88%), Positives = 68/71 (95%)

Query: 2 AFLASGPYLTHQQKVLRLYKRALRHLESWCVQRDKYRYFACLMRARFEEHKNEKDMAKAT 61

AFL+SG YLTHQQKVLRLYKRALRHLESWC+ RDKYRYFACL+RARF+EHKNEKDM KAT Sbjct: 1 AFLSSGAYLTHQQKVLRLYKRALRHLESWCIHRDKYRYFACLLRARFDEHKNEKDMVKAT 60

Query: 62 QLLKEAEEEFW 72

QLL+EAEEEFW Sbjct: 61 QLLREAEEEFW 71

>tr|U41534 | Q18036 | D34BCCB6E8FBCD5F (C16A3.4) SIMILAR TO

NADH-UBIQUINONE OXIDOREDUCTASE B22. [CAENORHABDITIS

ELEGANS]

Length = 163

Score = 52.7 bits (124), Expect = 3e-07

Identities = 25/64 (39%), Positives = 41/64 (64%), Gaps = 1/64 (1%)

Query 10 LTHQQKVLRLYKRALRHLESWCVQRD-KYRYFACLMRARFEEHKNEKDMAKATQLLKEAE 68

L+H+QKV RLYKR LR +++W + + R+ C++RARF+ + +E D K+ LL + Sbjct 12 LSHRQKVTRLYKRCLREVDNWYGGNNLEVRFQKCIIRARFDANADEVDTRKSQILLADGC 71 Query 69 EEFW 72

+ W Sbjct 72 RQLW 75

Alert BLASTP hits for DKFZphfkd2_3ol7, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphfkd2_3ol7, frame 2

Report for DKFZphfkd2_3ol7.2

[LENGTH] 72

[MW] 8839.28

[pi] 9.26

[HOMOL] PIR:S28256 NADH dehydrogenase (ubiquinone) (EC 1.6.5.3) chain CI-B22 - bovme

2e-34

[KW] All_Alpha

SEQ MAFLASGPYLTHQQKVLRLYKRALRHLESWCVQRDKYRYFACLMRARFEEHKNEKDMAKA PRD ccccccccchhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhhhhcchhhhhhh

SEQ TQLLKEAEEEFW PRD hhhhhhhhhccc

(No Prosite data available for DKFZphfkd2_3ol7.2 ) (No Pfam data available for DKFZphfkd2_3ol7.2) DKFZphfkd2 46a6

group: kidney derived

DKFZphf d2_46a6 encodes a novel 315 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of kidney-specific genes .

unknown complete cDNA, complete eds, EST hits

Sequenced by MediGenomix

Locus: /map="228.6 cR from top of Chrl5 linkage group"

Insert length: 2774 bp

Poly A stretch at pos. 2751, polyadenylation signal at pos. 2732

1 CTCGCGAGCG CAGCTATGGC TGCTGGCGTA CCCTGTGCGT TAGTCACCAG 51 CTGCTCCTCC GTCTTCTCAG GAGACCAGCT GGTCCAACAT ACCCTTGGAA

101 CAGAAGATCT TATTGTGGAA GTGACTTCCA ATGATGCTGT GAGATTTTAT

151 CCCTGGACCA TTGATAATAA ATACTATTCA GCAGACATCA ATCTATGTGT

201 GGTGCCAAAC AAATTTCTTG TTACTGCAGA GATTGCAGAA TCTGTCCAAG

251 CATTTGTGGT TTACTTTGAC AGCACACGAA AATCGGGCCT TGATAGTGTC

301 TCCTCATGGC TTCCACTGGC AAAAGCATGG TTACCTGAGG TGATGATCTT

351 GGTCTGCGAT AGAGTGTCTG AAGATGGTAT AAACCGACAA AAAGCTCAAG

401 AATGGAGCCT CAAACATGGC TTTGAATTGG TAGAACTTAG TCCAGAGGAG

451 TTGCCTGAGG AGGATGATGA CTTCCCAGAA TCTACAGGAG TAAAGCGAAT

501 TGTCCAAGCC CTGAATGCCA ATGTGTGGTC CAATGTAGTG ATGAAGAATG

551 ATAGGAACCA AGGCTTTAGC CTTCTCAACT CATTGACTGG AACAAACCAT

601 AGCATTGGGT CAGCAGATCC CTGTCACCCA GAGCAACCCC ATTTGCCAGC

651 AGCAGATAGT ACTGAATCCC TCTCTGATCA TCGGGGTGGT GCATCTAACA

701 CAACAGATGC CCAGGTTGAT AGCATTGTGG ATCCCATGTT AGATCTGGAT

751 ATTCAAGAAT TAGCCAGTCT TACCACTGGA GGAGGAGATG TGGAGAATTT

801 TGAAAGACCC TTTTCAAAGT TAAAGGAAAT GAAAGACAAG GCTGCGACGC

851 TTCCTCATGA GCAAAGAAAA GTGCATGCAG AAAAGGTGGC CAAAGCATTC

901 TGGATGGCAA TCGGGGGAGA CAGAGATGAA ATTGAAGGCC TTTCATCTGA

951 TGGAGAGCAC TGAATTATTC ATACTAGGGT TTGACCAACA AAGATGCTAG 1001 CTGTCTCTGA GATACCTCTC TACTCAGCCC AGTCATATTT TGCCAAAATT 1051 GCCCTTATCA TGTTGGCTGC CTGACTTGTT TATAGGGTCC CCTTAATTTT 1101 AGTTTTTAGT AGGAGGTTAA GGAGAAATCT TTTTTTTCCT CAGTATATTG 1151 TAAGAGAGTG AGGAATACAG TGATAGTAAT GAGTGAGGAT TTCTTAAATA 1201 TACTTTTTTT TTGTTCTAGG AATGAGGGTA GGATAAATCT CAGAGGTCTG 1251 TGTGATTTAC TCAAGTTGAA GACAACCTCC AGGCCATTCC TGGTCAACCT 1301 TTTAAGTAGC ATTTCCAGCA TTCACACTTG ATACTGCACA TCAGGAGTTG 1351 TGTCACCTTT CCTGGGTGAT TTGGGTTTTC TCCATTCAAG GAGCTTGTAG 1401 CTCTGAGCTA TGATGCTTTT ATTGGGAGGA AAGGAGGCAG CTGCAGAATT 1451 GATGTGAGCT ATGTGGGGCC GAAGTCTCAG CCCGCAGCTA AGTCTCTACC 1501 TAAGAAAATG CCTCTGGGCA TTCTTTTGAA GTATAGTGTC TGAGCTCATG 1551 CTAGAAAGAA TCAAAAAGCC AGTGTGGATT TTTAGGCTGT AATAAATGAG 1601 GCAAAGGATT TCTATTCCAG TGGGAAGGAA ACCTCTCTAC TGAGTTGTGG 1651 GGGATATGTT GTATGTTAGA GAGAACCTTA AGGAGTCCTT GTATGGGCCA 1701 TGGAGACAGT ATGTGATAAC ATACCGTGAT TTTCATGAAG AAATTCTTCT 1751 GTCCTAGAGT TCTCCCCTGC TGCTTGAGAT GCCAGAGCTG TGTTGTTGCA 1801 CACCTGCAAA ACAAGGCACA TTTCCCCCTT TCTCTTTAAA GCCAAAGAGA 1851 GATCACTGCC AAAGTGGGAG CACTAAGGGG TGGGTGGGGA AGTGAAATGT 1901 TAGGCGATGA ATTCCTGAGC ACCTTGTTTT TCTTCCAAGG TTCGTAGCTC 1951 CTCTCTGCCC TTCCAAGCCT GTAACCTCGG AGGACTATCT TTTGTTCTCT 2001 ATCCTTTGTC TTGTTAGAGT GGGTCAGCCC CAGAGGAACT GATAAGCAAA 2051 TGGCAAGTTT TTAAAGGAAG AGTGGAAAGT ACTGCAAATA AAAATCCTTA 2101 TTTGTTTTTG TAGACTTTGT AATGCATATC ATTAGCCCTC ACTGTGATCA 2151 TTACTGCTGT GGCTCTGAAC TGGCACATAG TACAGTGGAT GGAAGGTGCC 2201 CGCACACCAG CTGAGAACTG GTTCTGGCCT AGGTGGGCTC TAGAACCATT 2251 TACACAGCAT GAAAGAAACA GGTTGGGTTA GGAGCAGAAA GAAATAAGGC 2301 TCACACCCCT CCAGACACTA CCTTATAAGC ACTGCAGAAC CTGAAACAGA 2351 TGGCAGAAGG AATGGAATGC TACAGGGGCC AGCAGGAGTG ACCACAGGGA 2401 GGGGACAGCT CAGTGACTGG AGCATTCAGG AAGAGGCTTT CCAGGGAACA 2451 CTGGACATTG CTTAGTGACC TTTTGTTCCT TTTTTTTTTT TTTTCTTTTA 2501 CTGTTCTGAA AGACTTTGAG TCTGTGGTTC ACCACCAGCC CATCAGTGTT 2551 TCTTTGAGGT GATTGCATTA GGGAAGTTGG CTCTGGGATT GCAAAAAAAA 2601 AAAAAAGGTG GAACATGTTT TCCTTAAAAG ATGGAAGGTT TTAGAAAATA 2651 TACTAGGCCA TCTGGTTAGA AAAAACAGAC CAGACTAGAA AAAGCTGTGA 2701 ATTTGATTTT GTAGATTAAA CAAAGCCAGA TGATTAAAAT GTGATTTATT 2751 TATAAAAAAA AAAAAAAAAA AAAA

BLAST Results

Entry HS463358 from database EMBL: human STS WI-14364.

Length = 472

Minus Strand HSPs:

Score = 1605 (240.8 bits), Expect = 5.0e-68, P = 5.0e-68

Identities = 347/361 (96%)

Medline entries

No Medlme entry

Peptide information for frame 1

ORF from 16 bp to 960 bp; peptide length: 315 Category: putative protein Classification: unset

1 MAAGVPCALV TSCSSVFSGD QLVQHTLGTE DLIVEVTSND AVRFYPWTID

51 NKYYSADINL CVVPNKFLVT AEIAESVQAF VVYFDSTRKS GLDSVSSWLP

101 LAKAWLPEVM ILVCDRVSED GINRQKAQEW SLKHGFELVE LSPEELPEED

151 DDFPESTGVK RIVQALNANV WSNVVMKNDR NQGFSLLNSL TGTNHSIGSA

201 DPCHPEQPHL PAADSTESLS DHRGGASNTT DAQVDSIVDP MLDLDIQELA

251 SLTTGGGDVE NFERPFSKLK EMKDKAATLP HEQRKVHAEK VAKAFWMAIG

301 GDRDEIEGLS SDGEH

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfkd2_46a6, frame 1

PIR:T04362 probable GTP-bindmg protein yptm3 - maize, N = 1, Score = 87, P = 0.21

PIR:S71585 GTP-bindmg protein GB2 - Arabidopsis thaliana, N = 1, Score = 86, P = 0.27

>PIR:T04362 probable GTP-bmding protein yptm3 - maize Length = 210

HSPs:

Score = 87 (13.1 bits), Expect = 2.4e-01, P = 2.1e-01 Identities = 34/160 (21%), Positives = 67/160 (41%)

Query: 48 TIDNKYYSADINLCVVPNKFL-VTAEIAESVQAFVVYFDSTRKSGLDSVSSWLPLAKAWL 106

TIDNK I F +T ++ +D TR+ + ++SWL A+

Sbjct: 49 TIDNKPIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRETFNHLASWLEDARQHA 108

Query: 107 PE VMIL--VCDRVSEDGINRQKAQEWSLKHGFELVELSPEELPEEDDDFPESTGVKR 161

VM++ CD ++ ++ ++++ +HG +E S + ++ F ++ G Sbjct: 109 NANMTVMLIGNKCDLSHRRAVSYEEGEQFAKEHGLVFMEASAKTAQNVEEAFIKTAGT— 166

Query: 162 IVQALNANVWSNVVMKNDRNQGFSLLNSLTGTNHSIGSADPC 203

I + + ++ N G+++ NS G Ξ A C Sbjct: 167 IYKKIQDGIFDVSNESNGIKVGYAVPNSSGGGAGSSSQAGGC 208

Pedant information for DKFZphf d2_46a6, frame 1

Report for DKFZphfkd2_46a6.1

[LENGTH] 315 [MW] 34505.54 [pi] 4.55

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 6.67

SEQ MAAGVPCALVTSCSSVFSGDQLVQHTLGTEDLIVEVTΞNDAVRFYPWTIDNKYYSADINL

SEG

PRD cccccceeeeecccccccccceeeeccccceeeeeeccccceeeecccccccccccccee

SEQ CVVPNKFLVTAEIAESVQAFVVYFDSTRKSGLDSVΞSWLPLAKAWLPEVMILVCDRVSED

SEG

PRD eeecccchhhhhhhhhhheeeeeeecccccccccccccccccccccccceeeeccccccc

SEQ GINRQKAQEWSLKHGFELVELSPEELPEEDDDFPESTGVKRIVQALNANVWSNVVMKNDR

SEG xxxxxxxxxxxxxxxxxxxxx

PRD cchhhhhhhhhhcccceeeeccccccccccccccccccchhhhhhhhcccceeeeeeccc

SEQ NQGFSLLNSLTGTNHSIGSADPCHPEQPHLPAADSTESLSDHRGGASNTTDAQVDΞIVDP

SEG

PRD ccccccccccccccccccccccccccccccccccccccccccccccccccccccccccch

SEQ MLDLDIQELASLTTGGGDVENFERPFSKLKEMKDKAATLPHEQRKVHAEKVAKAFWMAIG

SEG

PRD hhhhhhhhhhhcccccccccccchhhhhhhhhhhhhhhccchhhhhhhhhhhhhhhhhhc

SEQ GDRDEIEGLSSDGEH

SEG

PRD ccccccccccccccc

(No Prosite data available for DKFZphfkd2_46a6.1) (No Pfam data available for DKFZphfkd2_46a6.1)

DKFZphfkd2_46bl0

group: kidney derived

DKFZphfkd2_46bl0.1 encodes a novel 315 ammo acid protein with similarity to C. elegans cosmide F25B5.3

The novel protein contains a HTH-LYSR-family PROSITE pattern. Proteins of the lysR family are bacterial transcriptional regulatory proteins which bind DNA using a helix-turn-helix motif. Most of these proteins are transcription activators and usually negatively regulate their own expression. They all possess a potential 'helix-turn-helix ' DNA-bmding motif m their N- terminal section. The ' helix-turn-helix ' motif is missing in DKFZphfkd2_46a6.1. No informative BLAST results, no predictive PFAM or SCOP motive.

The new protein can find application in studying the expression profile of kidney-specific genes . similarity to C. elegans F25B5.3 complete cDNA, complete eds, EST hits

Sequenced by MediGenomix

Locus: unknown

Insert length: 1285 bp

Poly A stretch at pos. 1266, no polyadenylation signal found

1 CAGTCTACGC GAGCTGCCTG TTTTTTTCCT GCTTGGACGC GCATGAGGGC

51 CCCGTCCATG GACCGCGCGG CCGTGGCGAG GGTGGGCGCG GTAGCGAGCG

101 CCAGCGTGTG CGCCCTGGTG GCGGGGGTGG TGCTGGCTCA GTACATATTC

_^151 ACCTTGAAGA GGAAGACGGG GCGGAAGACC AAGATCATCG AGATGATGCC

201 AGAATTCCAG AAAAGTTCAG TTCGAATCAA GAACCCTACA AGAGTAGAAG

251 AAATTATCTG TGGTCTTATC AAAGGAGGAG CTGCCAAACT TCAGATAATA

301 ACGGACTTTG ATATGACACT CAGTAGATTT TCATATAAAG GGAAAAGATG

351 CCCAACATGT CATAATATCA TTGACAACTG TAAGCTGGTT ACGGATGAAT

401 GTAGAAAAAA GTTATTGCAA CTAAAGGAAA AATATTACGC TATTGAAGTT

451 GATCCTGTTC TTACTGTAGA AGAGAAGTAC CCTTATATGG TGGAATGGTA

501 TACTAAATCA CATGGTTTGC TTGTTCAGCA AGCTTTACCA AAAGCTAAAC

551 TTAAAGAAAT TGTGGCAGAA TCTGACGTTA TGCTCAAAGA AGGATATGAG

601 AATTTCTTTG ATAAGCTCCA ACAACATAGC ATCCCCGTGT TCATATTTTC

651 GGCTGGAATC GGCGATGTAC TAGAGGAAGT TATTCGTCAA GCTGGTGTTT

701 ATCATCCCAA TGTCAAAGTT GTGTCCAATT TTATGGATTT TGATGAAACT

751 GGGGTGCTCA AAGGATTTAA AGGAGAACTA ATTCATGTAT TTAACAAACA

801 TGATGGTGCC TTGAGGAATA CAGAATATTT CAATCAACTA AAAGACAATA

851 GTAACATAAT TCTTCTGGGA GACTCCCAAG GAGACTTAAG AATGGCAGAT

901 GGAGTGGCCA ATGTTGAGCA CATTCTGAAA ATTGGATATC TAAATGATAG

951 AGTGGATGAG CTTTTAGAAA AGTACATGGA CTCTTATGAT ATTGTTTTAG

1001 TACAAGATGA ATCATTAGAA GTAGCCAACT CTATTTTACA GAAGATTCTA

1051 TAAACAAGCA TTCTCCAAGA AGACCTCTCT CCTGTGGGTG CAATTGAACT

1101 GTTCATCCGT TCATCTTGCT GAGAGACTTA TTTATAATAT ATCCTTACTC

1151 TCGAAGTGTT CCCTTTGTAT AACTGAAGTA TTTTCAGATA TGGTGAATGC

1201 ATTGACTGGA AGCTCCTTTT CTCCACCTCT CTCAACACAC TCCTCACCGT

1251 ATCTTTTAAC CCATTTAAAA AAAAAAAAAA AAAAA

BLAST Results

No BLAST result

Medlme entries

No Medline entry

Peptide information for frame 1

ORF from 43 bp to 1050 bp; peptide length: 336 Category: similarity to unknown protein Classif cation: unset Prosite motifs: HTH LYSR FAMILY (16-47) 1 MRAPSMDRAA VARVGAVASA SVCALVAGVV LAQYIFTLKR KTGRKTKIIE 51 MMPEFQKSSV RIKNPTRVEE IICGLIKGGA AKLQIITDFD MTLSRFSYKG 101 KRCPTCHNII DNCKLVTDEC RKKLLQLKEK YYAIEVDPVL TVEEKYPYMV 151 EWYTKSHGLL VQQALPKAKL KEIVAESDVM LKEGYENFFD KLQQHSIPVF 201 IFSAGIGDVL EEVIRQAGVY HPNVKVVSNF MDFDETGVLK GFKGELIHVF 251 NKHDGALRNT EYFNQLKDNS NIILLGDSQG DLRMADGVAN VEHILKIGYL 301 NDRVDELLEK YMDSYDIVLV QDESLEVANS ILQKIL

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfkd2_46bl0, frame 1

SWISSPROT :YQT3_CAEEL HYPOTHETICAL 42.0 KD PROTEIN F25B5.3 IN CHROMOSOME III., N = 1, Score = 524, P = 2.2e-50

TREMBL :AC005499_12 gene: "T6A23.12"; Arabidopsis thaliana chromosome II BAC T6A23 genomic sequence, complete sequence., N = 2, Score = 194, P = 1.4e-26

>SWISSPROT:YQT3_CAEEL HYPOTHETICAL 42.0 KD PROTEIN F25B5.3 IN CHROMOSOME III.

Length = 376

HSPs:

Score = 524 (78.6 bits), Expect = 2.2e-50, P = 2.2e-50 Identities = 112/300 (37%), Positives = 174/300 (58%)

Query: 44 RKTKIIEMMPEFQ—KSSVRIKNPTRVEEIICGLIKGGAAKLQIITDFDMTLSRFSYK-G 100

+KT ++ ++ + + + + +PT V + ++ GGA K +I+DFD TLSRF+ + G Sbjct: 73 KKTDVVPLLMNYLLGEEQILVADPTAVAAKLRKMVVGGAGKTVVISDFDYTLSRFANEQG 132

Query: 101 KRCPTCHNIID-NCKLVTDECRKKLLQLKEKYYAIEVDPVLTVEEKYPYMVEWYTKSHGL 159

+R T H + D N + E +K + LK KYY IE P LT+EEK P+M +W+ SH L Sbjct: 133 ERLSTTHGVFDDNVMRLKPELGQKFVDLKNKYYPIEFSPNLTMEEKIPHMEKWWGTSHSL 192

Query: 160 LVQQALPKAKLKEIVAESDVMLKEGYENFFDKLQQHSIPVFIFSAGIGDVLEEVIRQA-G 218

+V + K +++ V +S ++ K+G E+F + L H+IP+ IFSAGIG+++E ++Q G Sbjct: 193 IVNEKFSKNTIEDFVRQSRIVFKDGAEDFIEALDAHNIPLVIFSAGIGNIIEYFLQQKLG 252

Query: 219 VYHPNVKVVSNFMDFDETGVLKGFKGELIHVFNKHDGAL-RNTEYFNQLKDNSNIILLGD 277

N +SN + FDE F LIH F K+ + + T +F+ + N+ILLGD Sbjct: 253 AIPRNTHFISNMILFDEDDNACAFSEPLIHTFCKNSSVIQKETSFFHDIAGRVNVILLGD 312

Query: 278 SQGDLRMADGVANVEHILKIGYLNDRVDEL—LEKYMDSYDIVLVQDESLEVANSILQKI 335

S GD+ M GV LK+GY N +D+ L+ Y + YDIVL+ D +L VA 1+ I Sbjct: 313 SMGDIHMDVGVERDGPTLKVGYYNGSLDDTAALQHYEEVYDIVLIHDPTLNVAQKIVDII 372

Pedant information for DKFZphfkd2_46bl0, frame 1

Report for DKFZphfkd2_46bl0.1

[LENGTH] 336

[MW] 37948.37

[pi] 6.67

[HOMOL] SWISSPROT :YQT3_CAEEL HYPOTHETICAL 42.0 KD PROTEIN F25B5.3 IN CHROMOSOME III.

3e-51

[PROSITE] HTH_LYΞR_FAMILY 1

[KW] TRANSMEMBRANE 2

[KW] LOW COMPLEXITY 7.44 %

SEQ MRAPSMDRAAVARVGAVASASVCALVAGVVLAQYIFTLKRKTGRKTKIIEMMPEFQKSSV

SEG xxxxxxxxxxxxxxxxxxxxxxxxx

PRD cccchhhhhcchhhhhhheeehhhhhhhhhhhhhhhhhhhhhccceeeehhhhhhhhhee

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ RIKNPTRVEEIICGLIKGGAAKLQIITDFDMTLSRFSYKGKRCPTCHNIIDNCKLVTDEC SEG PRD eecccchhhhhhhhhhccccceeeeecccccceeeecccccccccccccccccchhhhhh

MEM SEQ RKKLLQLKEKYYAIEVDPVLTVEEKYPYMVEWYTKSHGLLVQQALPKAKLKEIVAESDVM

SEG

PRD hhhhhhhhhhhheeeccccccccccchhhhhhccccchhhhhhccchhhhhhhhhhhhcc

MEM

SEQ LKEGYENFFDKLQQHSIPVFIFSAGIGDVLEEVIRQAGVYHPNVKVVSNFMDFDETGVLK

SEG

PRD ccccchhhhhhhhhcccceeeeecccchhhhhhhhhhcccccceeeeeecccccccccee

MEM MMMMMMMMMMMMMMMMMMM

SEQ GFKGELIHVFNKHDGALRNTEYFNQLKDNΞNIILLGDSQGDLRMADGVANVEHILKIGYL

SEG

PRD eccceeeeeeecccccccccchhhhhhhhceeeeecccccccccccccccccceeeeeec

MEM

SEQ NDRVDELLEKYMDSYDIVLVQDESLEVANSILQKIL

SEG

PRD cchhhhhhhhhhhhheeeeeecchhhhhhhhhhccc

MEM

Prosite for DKFZphfkd2_46bl0.1 PS00044 16->47 HTH_LYSR_FAMILY PDOC00043

(No Pfam data available for DKFZphfkd2_46bl0.1)

DKFZphfkd2_46dl3

group: kidney derived

DKFZphfkd2_46dl3 encodes a novel 506 amino acid protein with weak similarity to KE03 protein

The novel protein contains a RGD site.

No informative BLAST results; No predictive prosite, pfam or SCOP motive

The new protein can find application in studying the expression profile of kidney-specific genes .

similarity to KE03 protein complete cDNA, complete eds, EST hits

Sequenced by MediGenomix

Locus: /map="227.6 cR from top of Chrl linkage group"

Insert length: 3346 bp

Poly A stretch at pos. 3328, polyadenylation signal at pos. 330E

1 CTCTCGCGAG AGGAGCAAGA GGAAGATGGC CGTGCCCTGT TTTTCGGTGT

51 AAGGCAGCAG ACGGCGGCTG CGACGGCGAG ACTGAGATCC TGGTGTCGTG

101 GGCACCTGAG TTCTAGCTTC CCCCAGCGAG CGCGCGTCCC TTCGTGCCTA

151 GGCGAGAGCC GGCTCTTCCC CGGGAGATGC GTTTGTCCCA GGCTCGGGGG

201 CTCAGTGGGA GTTCATGCTG CGCTGGAGGC TCTTGGCCAC CGCTCTAATC

251 GCCTTGTGCC GCCGCAGCGC CAGCTCCGTC GCCAGCGGTG AGCCTCCCGA

301 TTCCCCCCCT TGCCCCTGGC GGCGGCGATG ACCGGGGAGA AGATCCGCTC

351 ACTGCGGAGG GACCACAAGC CCAGCAAAGA AGAAGGGGAC CTGCTGGAGC

401 CCGGGGATGA AGAAGCGGCG GCTGCCCTCG GCGGTACCTT TACCAGAAGC

451 AGGATTGGCA AGGGCGGCAA AGCTTGTCAT AAGATCTTCA GTAACCATCA

501 CCACCGGCTA CAGCTGAAGG CAGCTCCGGC CTCCTCCAAT CCCCCCGGCG

551 CCCCGGCTCT GCCGCTGCAC AATTCCTCCG TGACTGCCAA CTCCCAGTCC

601 CCGGCCCTTC TGGCCGGCAC CAACCCCGTT GCTGTCGTCG CGGATGGAGG

651 CAGTTGCCCC GCACACTACC CGGTGCACGA GTGCGTCTTC AAGGGGGATG

701 TGAGGAGACT CTCCTCTCTC ATCCGCACGC ACAATATCGG GCAGAAAGAT

751 AATCACGGAA ATACTCCTTT ACACCTTGCT GTGATGTTAG GAAATAAAGT

801 TACAGCTCTT TTGAGGAAGC TTAAGCAGCA ATCCAGGGAA AGTGTTGAAG

851 AAAAACGACC TCGATTATTA AAAGCCCTGA AAGAGCTAGG TGACTTTTAT

901 CTAGAACTTC ACTGGGATTT TCAAAGCTGG GTGCCTTTAC TTTCCCGAAT

951 TCTGCCTTCC GATGCATGTA AAATATACAA ACAAGGTATC AATATCAGGC

1001 TTGACACAAC TCTCATAGAC TTTACTGACA TGAAGTGCCA ACGAGGGGAT

1051 CTAAGCTTCA TTTTCAATGG GGATGCGGCG CCCTCTGAAT CTTTTGTAGT

1101 ATTAGACAAT GAACAAAAAG TTTATCAGCG AATACATCAT GAGGAATCAG

1151 AGATGGAAAC AGAAGAAGAG GTGGATATTT TAATGAGCAG TGATATTTAC

1201 TCTGCAACTT TATCAACAAA ATCAATTTCT TTCACGCGTG CCCAGACAGG

1251 ATGGCTTTTT CGGGAAGATA AAACAGAAAG AGTAGGAAAC TTTTTGGCAG

1301 ACTTTTACCT GGTGAATGGA CTTGTTATAG AATCAAGGAA AAGAAGAGAA

1351 CATCTCAGTG AAGAGGATAT TCTTCGAAAT AAGGCCATCA TGGAGAGTTT

1401 GAGTAAAGGT GGAAACATAA TGGAACAGAA TTTTGAGCCG ATTCGAAGAC

1451 AGTCTCTTAC ACCGCCTCCT CAGAACACTA TTACATGGGA AGAATATATA

1501 TCTGCTGAAA ATGGAAAAGC TCCTCATCTG GGTAGAGAAT TGGTGTGCAA

1551 AGAGAGTAAG AAAACGTTTA AAGCTACGAT AGCCATGAGC CAGGAATTTC

1601 CCTTAGGGAT AGAGTTATTA TTGAATGTTT TAGAAGTAGT AGCTCCCTTC

1651 AAGCACTTTA ACAAGCTTAG AGAATTTGTT CAGATGAAGC TTCCTCCAGG

1701 CTTTCCTGTA AAATTAGATA TACCTGTGTT TCCCACAATC ACAGCCACTG

1751 TGACTTTTCA GGAGTTTCGA TACGATGAAT TTGATGGCTC CATCTTTACT

1801 ATACCTGATG ACTACAAGGA AGACCCAAGC CGTTTTCCTG ATCTTTAACT

1851 GACGTGGAAA AGGATGCCGT CTAACCAAGG AAAGAAAATA CAGAGACCCT

1901 AGAAGTGGAT CCAAATAGAA GGGACAAATG CTTTCAGTGA AGAAAAGGGA

1951 ATTACACATT GAATCGACAC ATCAGTAATA CGATACAGTG AAATGGGCCT

2001 CTAATAAGAA TTTCAGCGAG TTTTCTGATG TGCCATTTTT TGTCTTTTTA

2051 AAAATATACA TATTATAAAT GTAATAGTTT GACACATTAA TGACCCTAAG

2101 ACCTGCGTAT GTGAAGCAGC TATGAGTGCT GTGATTTGTT TTTAAAAATT

2151 TTTACACTTC TTGTTGAAAT ATATATGCAT ATAAATATAT CTATATCTAT

2201 ATCTATATCT AAAACACTCC TGGACCATTA ACGTAAATTA AATGTCTTAA

2251 GAGATATGGA GCCCTTTTAA ACTTGTCATC TTTATGCAAG GTGACATTTA

2301 TAAATATTCC TTCGAGCTTT GTTTTCATAA AATGTAAACT ATGTAACATT

2351 ATGTATAGTT CAGTAATTTG AATGTTTGTT CAATATAATG AACTAGAAGG

2401 AATGCAATTT TCTGTAGATG AATGAACCAA ATGGTAACCA TTAAACAATT

2451 GCATTTATAT GTTGCAATAC ATTTCAGAAG GAGCGTTCAC TCTGCAGGGA

2501 ATAAGGTACC TCCTTTAGCA CCTTAGTGCA ATTCATTGTG GTGCTATTTG

2551 TTTTTACCTG AATGTTTGTT ACTAATCTTC CTTTCATAGA ACCTCTATTT

2601 TTTTTTTTTC TAAACTTGAG TTTGAGTCCT TGTTATGGTC ATCATAAGGT 2651 AATGGTTAGC ATGTTTAAAG ATATTCCTCT TCCAAATCTC AGCACTTTAA

2701 AAAAAAATCC AAATTTTTAA ACTTGCTTCC TAATAAGTAC ACATCGGTCT

2751 GATTATTTTG TTTGTTTTTA GTAGAATATG GATGCATTGG TGTCAGTTTT

2801 AAAAAACAAT ACACATATTT TGGACAACCC TACATATTTA ATCCTTTCAA

2851 AATAAGATAA AAACATTTTA TATGCTAACA GAATATATTT GTTACAAGTT

2901 AAAGTCCAGA AGTATACACA AGATTGATTA CTCCTATTAT TTTTTTTAAA

2951 TCACAGGAAA ATATTGATTT CATTGTCTCC AAAGTGATAA AATCTTGTAT

3001 TACTCATTTT TGCACTTAAA ATTTTTCTTA TTTATTCCAA GGTGGTTTGA

3051 AGGTCCAAGT ATGAAAATAA ATTAGGGGGA TTAATGTATA ACAGTTATAA

3101 AGTATCATGT TGTATTAAAG AGCTTACTTA GATTGATGTT TTTAAAATGT

3151 ATCCTGATGA ATGTCTCAAG AATGCATCTG TCAAGTTTTT TAGACTGACC

3201 AGTAGCTTAA ACTTTTTTCA GGATTTTAGG TAATTTGAAA GGAGTTTAGA

3251 GACCCTTATT GAAAATATGA TTTAAAAATC CAAAGCATAA ACCGTAAGAA

3301 AAATTTTAAA TAAACATCTT TAAAGCTGAA AAAAAAAAAA AAAAAA

BLAST Results

Entry HS121353 from database EMBL: human STS WI-14729. Score = 1697, P = 1.9e-69, identities 363/379

Medline entries

No Medline entry

Peptide information for frame 1

ORF from 328 bp to 1845 bp; peptide length: 506 Category: similarity to unknown protein

1 MTGEKIRSLR RDHKPSKEEG DLLEPGDEEA AAALGGTFTR SRIGKGGKAC 51 HKIFSNHHHR LQLKAAPASS NPPGAPALPL HNSSVTANSQ SPALLAGTNP 101 VAVVADGGSC PAHYPVHECV FKGDVRRLSS LIRTHNIGQK DNHGNTPLHL 151 AVMLGNKVTA LLRKLKQQSR ESVEEKRPRL LKALKELGDF YLELHWDFQS 201 WVPLLSRILP SDACKIYKQG INIRLDTTLI DFTDMKCQRG DLSFIFNGDA 251 APSESFVVLD NEQKVYQRIH HEESEMETEE EVDILMSSDI YSATLSTKSI 301 SFTRAQTGWL FREDKTERVG NFLADFYLVN GLVIESRKRR EHLSEEDILR 351 NKAIMESLSK GGNIMEQNFE PIRRQSLTPP PQNTITWEEY ISAENGKAPH 401 LGRELVCKES KKTFKATIAM SQEFPLGIEL LLNVLEVVAP FKHFNKLREF 451 VQMKLPPGFP VKLDIPVFPT ITATVTFQEF RYDEFDGSIF TIPDDYKEDP 501 SRFPDL

BLASTP hits

Entry CEC01F1_3 from database TREMBL: gene: "C01F1.6"; Caenorhabditis elegans cosmid C01F1.

Score = 371, P = 4.5e-61, identities = 69/138, positives = 96/138

Entry CEC18F10_9 from database TREMBL: gene: "C18F10.7"; Caenorhabditis elegans cosmid C18F10.

Score = 383, P = 3.4e-39, identities = 103/349, positives = 182/349

Entry AF064604_1 from database TREMBL: product: "KE03 protein"; Homo sapiens KE03 protein mRNA, partial eds.

Score = 348, P = 8.3e-32, identities = 95/295, positives = 148/295

Alert BLASTP hits for DKFZphfkd2_46dl3, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphf d2_46dl3, frame 1

Report for DKFZphfkd2_46dl3.1

[LENGTH] 506

[MW] 57003.12

[pi] 6.40 [HOMOL] TREMBL :CEC18F10_9 gene: "C18F10.7"; Caenorhabditis elegans cosmid C18F10. 2e-35

[BLOCKS] BL01288E

[PROSITE] RGD 1

[PROSITE] MYRISTYL 7

[PROSITE] CAMP_PHOSPHO_SITE 2

[PROSITE] CK2_PHOΞPHO_SITE 9

[PROSITE] PKC_PHOSPHO_SITE 6

[PROSITE] ASN_GLYCOΞYLATION 1

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 7.51 '

SEQ MTGEKIRSLRRDHKPSKEEGDLLEPGDEEAAAALGGTFTRSRIGKGGKACHKIFSNHHHR SEG xxxxxxxxxxxx PRD ccceeeeeccccccccccccccccccchhhhhhhccccccccccccceeeeeeecchhhh

SEQ LQLKAAPASSNPPGAPALPLHNSSVTANSQSPALLAGTNPVAVVADGGSCPAHYPVHECV SEG .... xxxxxxxxxxxxxxxx PRD hhhhhhccccccccceeecccccccccccccceeecccccceeeecccccccccccceee

SEQ FKGDVRRLSSLIRTHNIGQKDNHGNTPLHLAVMLGNKVTALLRKLKQQSRESVEEKRPRL SEG PRD eccchhhhhhhhhhcccccccccccccceeeecccchhhhhhhhhhhhcchhhhhhhhhh

SEQ LKALKELGDFYLELHWDFQSWVPLLSRILPSDACKIYKQGINIRLDTTLIDFTDMKCQRG SEG PRD hhhhhhccccceeehhhhhccceeeeccccccceeeeeccceeeeeeeeecccccccccc

SEQ DLSFIFNGDAAPSESFVVLDNEQKVYQRIHHEESEMETEEEVDILMSSDIYSATLSTKSI SEG xxxxxxxxxx PRD ceeeeeccccceeeeeeeecccceeeehhhhhhhhhhhhhhhhhhhhccceeeecccccc

SEQ SFTRAQTGWLFREDKTERVGNFLADFYLVNGLVIESRKRREHLSEEDILRNKAIMESLSK SEG PRD eeeecccceeeecccchhhhhhheeeeeeeeeeeeehhhhhhhhhhhhhhhhhhhhhhhc

SEQ GGNIMEQNFEPIRRQSLTPPPQNTITWEEYISAENGKAPHLGRELVCKESKKTFKATIAM SEG PRD cceeeccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhhh

SEQ SQEFPLGIELLLNVLEVVAPFKHFNKLREFVQMKLPPGFPVKLDIPVFPTITATVTFQEF SEG PRD hhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccceeeeeeeeeeehhhhhhhcc

SEQ RYDEFDGSIFTIPDDYKEDPSRFPDL SEG PRD cccccccceeeccccccccccccccc

Prosite for DKFZphfkd₂_46dl3.1

PS00001 82->86 ASN_GLYCOSYLATION PDOC00001 PS00004 126->130 CAMP_PHOSPHO_SITE PDOC00004 PS00004 373->377 CAMP_PHOSPHO_SITE PDOC00004 PS00005 8->ll PKC_PHOSPHO_SITE PDOC00005 PS00005 296->299 PKC_PHOSPHO_SITE PDOC00005 PS00005 316->319 PKC_PHOSPHO_SITE PDOC00005 PS00005 336->339 PKC_PHOSPHO_SITE PDOC00005 PS00005 410->413 PKC_PHOSPHO_SITE PDOC00005 PS00005 413->416 PKC_PHOSPHO_SITE PDOC00005 PS00006 16->20 CK2_PHOSPHO_SITE PDOC00006 PS00006 172->176 CK2_PHOSPHO_SITE PDOC00006 PS00006 228->232 CK2_PHOSPHO_SITE PDOC00006 PS00006 274->278 CK2_PHOSPHO_SITE PDOC00006 PS00006 278->282 CK2_PHOSPHO_SITE PDOC00006 PS00006 344->348 CK2_PHOSPHO_SITE PDOC00006 PS00006 386->390 CK2_PHOSPHO_SITE PDOC00006 PS00006 476->480 CK2_PHOSPHO_SITE PDOC00006 PS00006 491->495 CK2_PHOSPHO_ΞITE PDOC00006 PS00008 35->41 MYRISTYL PDOC00008 PS00008 46->52 MYRISTYL PDOC00008 PS00008 108->114 MYRISTYL PDOC00008 PS00008 138->144 MYRISTYL PDOC00008 PS00008 155->161 MYRISTYL PDOC00008 PS00008 320->326 MYRISTYL PDOC00008 PS00008 487->493 MYRISTYL PDOC00008 PS00016 239->242 RGD PDOC00016

(No Pfam data available for DKFZphfkd2_46dl3.1) DKFZphfkd2_46j20

group: metabolism

DKFZphfkd2_346j20 encodes a novel 224 amino acid protein similar to 2-hydroxyhepta-2, 4-dιene- 1,7-dιoate isomerase.

The new protein seems to be the human ortholog of 2-hydroxyhepta-2, 4-dιene-l, 7-dιoate isomerase .

The new protein can find application in modulating the homoprotocatechuate degradative pathway and as a enzyme for biotechnologic production processes. strong similarity to 2-hydroxyhepta-2, 4-dιene-l, 7-dιoate isomerase complete cDNA, complete eds, EST hits, potential start at Bp 16 matches kozak consensus ANCatgG strong similarity to proteins of worm plant archea and bacteria

2-hydroxyhepta-2, 4-dιene-l, 7-dιoate isomerase is part of the tyrosine metabolism (degradation of tyrosine late step) EC 5.3.1.- complete eds according to similar C. elegans and A. thaliana protein

Sequenced by MediGenomix

Locus : unknown

Insert length: 1706 bp

Poly A stretch at pos. 1686, polyadenylation signal at pos. 1667

1 CACTTGATGG GAATCATGGC AGCATCCAGG CCATTGTCCC GCTTCTGGGA

51 GTGGGGAAAG AACATCGTCT GCGTGGGGAG GAACTACGCG GACCACGTCA

101 GGGAGATGCG CAGCGCGGTG TTGAGCGAGC CCGTGCTGTT CCTGAAGCCG

151 TCCACGGCCT ACGCGCCCGA GGGCTCGCCC ATCCTCATGC CCGCGTACAC

201 TCGCAACCTG CACCACGAGC TGGAGCTGGG CGTGGTGATG GGCAAGCGCT

251 GCCGCGCAGT CCCCGAGGCT GCGGCCATGG ACTACGTGGG CGGCTATGCC

301 CTGTGCCTGG ATATGACCGC CCGGGACGTG CAGGACGAGT GCAAGAAGAA

351 GGGGCTGCCC TGGACTCTGG CGAAGAGCTT CACGGCGTCC TGCCCGGTCA

401 GCGCGTTCGT GCCCAAGGAG AAGATCCCTG ACCCTCACAA GCTGAAGCTC

451 TGGCTCAAGG TCAACGGCGA ACTCAGACAG GAGGGTGAGA CATCCTCCAT

501 GATTTTTTCC ATCCCCTACA TCATCAGCTA TGTTTCTAAG ATCATAACCT

551 TGGAAGAAGG AGATATTATC TTGACTGGGA CGCCAAAGGG AGTTGGACCG

601 GTTAAAGAAA ACGATGAGAT CGAGGCTGGC ATACACGGGC TGGTCAGTAT

651 GACATTTAAA GTGGAAAAGC CAGAATATTG AGTTATTTCT TAACAAGTTT

701 CGAGAGAGAA GGGAGCAAGA CAAGAGCAAG CAACGGCTAT TAAATGTCAC

751 AATCCTTTAA TTAGAAACCA TTTATTGGCC GGACGCGGTG GCTCACGCCT

801 GTAATCGCAG CACTTTGGGA GGCCGAGGCG GGCGGCTCAC GACGTCAGGA

851 GATCCAGACC ATCTTGGCTA ACAGGGTGAA ACCCCGTCTC TACTAAAAAT

901 ACAAAAAATT AGCCGGGCGT GGTGGCGGGC GCCTGTAGTC CCAGCTACTC

951 TGGAGGCTGA GGCAGGAGAA TCAATTGAAC CCGGGAGGCG GAGCTTACAG

1001 TGAGCTGAGA TTGCGCCACT GTACTCCTGG GCAACAGCGA GACTCCGTCT

1051 CAAAAAAAAA AAAAAAAAAA AGAAACCATT TATTTTAAAA ATGATTAGAT

1101 TGCTATGCCT CAACTCATAG AAGATGAACC CTTCAAGAAA ACGTGAAGTA

1151 GAACGGGTGG GCCAGAAATG AAAACAGGCA AGTAAAGTAT TTCTTCGGAA

1201 AACATTTTAT CAAACCAAAT GTTAAAAAGA CTTTCCTTTT GTAAAACTGG

1251 ATTAGAGAAG ACTTTTCAGT GGGTTATCTC TAGGATGATC AGTAGTTCAG

1301 CACTTAAAAA CTGCAGAGAA AACTGAAAGT TATGTTCCAG ATAACTTTCC

1351 GTTGTTTACC AAATTTTCTT AGATTTGGTC ATCATCAGGA AGCATTTGTA

1401 AAAATAAAAA TCTCCACAAA TTACTGGCCC ATCTCGGACT TGCTGAATCA

1451 ATTTGATAGG ATTAATCTCC AGTGAAGCTG TGTTTACAGG GCATTCCAAG

1501 TGATTCTTAT CAGGAAATGT GAAAAACACT CCTGTACATA ATCGGTTAAT

1551 TTAAAATTTT ACTTAATAAG TGAACAAGTA ATGAAGATTT CACCTGTTTA

1601 CTTAGGGTAT CTACCCAGAC CCATCGATTC TGAGTTCGGG AGATGATTTT

1651 GAAATTACTG TTTTCCAAAT AAAGGTGCTC CCTTCCAAAA AAAAAAAAAA

1701 AAAAAA

BLAST Results

No BLAST result

Medline entries 94039092: Purification, nucleotide sequence and some properties of a bifunctional isomerase/deearboxylase from the homoprotocatechuate degradative pathway of Escherichia coli C.

Peptide information for frame 1

ORF from 7 bp to 678 bp; peptide length: 224 Category: strong similarity to known protein

1 MGIMAASRPL SRFWEWGKNI VCVGRNYADH VREMRSAVLS EPVLFLKPST

51 AYAPEGSPIL MPAYTRNLHH ELELGVVMGK RCRAVPEAAA MDYVGGYALC

101 LDMTARDVQD ECKKKGLPWT LAKSFTASCP VSAFVPKEKI PDPHKLKLWL

151 KVNGELRQEG ETΞSMIFSIP YIISYVSKII TLEEGDIILT GTPKGVGPVK

201 ENDEIEAGIH GLVSMTFKVE KPEY

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfkd2_46j20, frame 1

PIR:S44919 ZK688.3 protein - Caenorhabditis elegans, N = 1, Score = 537, P = 8.7e-52

PIR:D71109 probable 2-hydroxyhepta-2, 4-dιene-l, 7-dιoate isomerase - Pyrococcus horikoshn, N = 1, Score = 529, P = 6.1e-51

PIR:C71425 hypothetical protein - Arabidopsis thaliana, N = 1, Score = 519, P = 7e-50

PIR:A64864 probable 2-hydroxyhepta-2, 4-dιene-l, 7-dιoate isomerase bll80 - Escherichia coli, N = 1, Score = 474, P = 4.1e-45

>PIR:S44919 ZK688.3 protein - Caenorhabditis elegans Length = 214

HSPs:

Score = 537 (80.6 bits), Expect = 8.7e-52, P = 8.7e-52 Identities = 99/211 (46%), Positives = 138/211 (65%)

Query: 10 LSRFWEWGKNIVCVGRNYADHVREMRSAVLSEPVLFLKPSTAYAPEGSPILMPAYTRNLH 69

L+ F IVCVGRNY DH E+ +A+ +P+LF+K ++ EG PI+ P +NLH Sbjct: 4 LAGFRNLATKIVCVGRNYKDHALELGNAIPKKPMLFVKTVNSFIVEGEPIVAPPGCQNLH 63

Query: 70 HELELGVVMGKRCRAVPEAAAMDYVGGYALCLDMTARDVQDECKKKGLPWTLAKSFTASC 129

E+ELGVV+ K+ + ++ AMDY+GGY + LDMTARD QDE KK G PW LAKSF SC Sbjct: 64 QEVELGVVISKKASRISKSDAMDYIGGYTVALDMTARDFQDEAKKAGAPWFLAKSFDGSC 123

Query: 130 PVSAFVPKEKIPDPHKLKLWLKVNGELRQEGETSSMIFSIPYIISYVSKIITLEEGDIIL 189

P+ F+P IP+PH ++L+ K+NG+ +Q T MIF IP ++ Y ++ TLE GD++L Sbjct: 124 PIGGFLPVSDIPNPHDVELFCKINGKDQQRCRTDVMIFDIPTLLEYTTQFFTLEVGDVVL 183

Query: 190 TGTPKGVGPVKENDEIEAGIHGLVΞMTFKVE 220

TGTP GV + D IE G+ ++ F V+ Sbjct: 184 TGTPAGVTKINSGDVIEFGLTDKLNSKFNVQ 214

Pedant information for DKFZphfkd2_46j20, frame 1

Report for DKFZphf d2_46j 20.1

[LENGTH] 224

[MW] 24843.07

[pi] 6.96

[HOMOL] PIR:S44919 ZK688.3 protein - Caenorhabditis elegans 8e-55

[FUNCAT] r general function prediction [M. jannaschn, MJ1656] 9e-40

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YNL168c] 4e-38

[EC] 5.3.3.10 5-Carboxymethyl-2-hydroxymuconate delta-isomerase le-35

[PIRKW] isomerase le-35

[PIRKW] intramolecular oxidoreductase le-35

[SUPFAM] 2-hydroxyhepta-2, 4-dιene-l, 7-dιoate isomerase le-46

[PROSITE] MYRISTYL 4

[PROSITE] AMIDATION 1 [PROSITE] CK2_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 3 [KW] Alpha_Beta

SEQ MGIMAASRPLSRFWEWGKNIVCVGRNYADHVREMRSAVLSEPVLFLKPSTAYAPEGSPIL PRD cccccccccchhhhhhcceeeeeecchhhhhhhhhccccccceeeecccccccccccccc

SEQ MPAYTRNLHHELELGVVMGKRCRAVPEAAAMDYVGGYALCLDMTARDVQDECKKKGLPWT PRD cccccchhhhhhheeeccccccccchhhhhhhheeeeeeccchhhhhhhhhhhhcccccc

SEQ LAKSFTASCPVSAFVPKEKIPDPHKLKLWLKVNGELRQEGETSSMIFSIPYIISYVSKII PRD cccccccccccceeeecccccccccceeeeecccccccccccccceeechhhhhhhhhhh

SEQ TLEEGDIILTGTPKGVGPVKENDEIEAGIHGLVSMTFKVEKPEY PRD hccccceeeeccccccccccccceeeeeeccccccccccccccc

Prosite for DKFZphfkd2_46j 20.1

PS00005 104->107 PKC_PHOSPHO_SITE PDOC00005 PS00005 192->195 PKC_PHOSPHO_SITE PDOC00005 PS00005 216->219 PKC_PHOSPHO_SITE PDOC00005 PS00006 104->108 CK2_PHOSPHO_SITE PDOC00006 PS00006 181->185 CK2_PHOSPHO_SITE PDOC00006 PS00008 2->8 MYRISTYL PDOC00008 PS00008 75->81 MYRISTYL PDOC00008 PS00008 116->122 MYRISTYL PDOC00008 PS00008 191->197 MYRISTYL PDOC00008 PS00009 78->82 AMIDATION PDOC00009

(No Pfam data available for DKFZphfkd2_46 20.1)

DKFZphfkd2_46kl9

group: transcription factors

DKFZphfkd2_46kl9.3 encodes a novel 130 ammo acid protein similar to rat Dcoh, a bifunctional protein-bmding transcriptional co-activator .

Dcoh is a bifunctional protein, complexed with biopterin. It serves as dimerization cofactor of hepatocyte nuclear factor-1 and catalyzes the dehydration of the biopterin cofactor of phenylalanine hydroxylase .

The new protein can find application in modulating/blocking the expression of genes controlled by the hepatocyte nuclear factor-1. strong similarity to pterm-4-alpha-carbιnolamιne dehydratase potential start at Bp 102 according to similar proteins, both genomic sequences are from chromosome 5,

Sequenced by MediGenomix

Locus: map="5"

Insert length: 5641 bp

Poly A stretch at pos. 5617, polyadenylation signal at pos. 5598

1 CAGCCCTCGG CAGACGGCCA ATGGCGGCGG TGCTCGGGGC GCTCGGGGCG

51 ACGCGGCGCT TGTTGGCGGC GCTGCGAGGC CAGAGCCTAG GGCTAGCGGC

101 CATGTCATCA GGTACTCACA GGTTGATTGC AGAGGAGAGG AACCAAGCTA

151 TACTTGACCT TAAAGCAGCA GGATGGTCGG AATTAAGTGA GAGAGATGCC

201 ATCTACAAAG AATTCTCCTT CCACAATTTT AATCAGGCAT TTGGCTTTAT

251 GTCCCGAGTT GCCCTACAAG CAGAGAAGAT GAATCATCAC CCAGAATGGT

301 TCAATGTATA CAACAAGGTC CAGATAACTC TCACCTCACA TGACTGTGGT

351 GAACTGACCA AAAAAGATGT GAAGCTGGCC AAGTTTATTG AAAAAGCAGC

401 TGCTTCTGTG TGATTTCTTC CAAAATACAT AAGTCTGAGA GGCTAAACTT

451 GATGGCTGTG TTAACATATG TCACGTGTAG CACAGTGGAG AAAGCAGGAT

501 ATGGCTCATA ATGACAGTGG TGAAGACCTG CGAATGAAGT TGCTAGTTAA

551 CACCTACATT AGGGTTTGAC ATAGGTCTAT GTTATGGGTC GCTGCATCTG

601 CTGGAACTCA CAGACTTTAC TATAGAGAAT CAAAGATCCC GTATCCGAAG

651 TCTATGGAAA TGCTCATGGT GGTAAATTCC AACAGAATGA AACACCAAAC

701 TTGCTTAAAG TAACTCACGT TTCAATTTGA AAGAGATATT GTCAAAATTG

751 GAGGCCCCCA GGTTCCTGTC TGTTCCAAAT CTTTGCATGA TGACAGTGGT

801 TTCTCTGATG TGGTAAGCTT TGGCTTTCTT CTGTTTTCTT TCTAAAAGAT

851 CACTGGAGTA GAGAGGAGTT AAACAGACAT GACCTTTGAC CTCTTGCATG

901 ACCTCCACAG ATAGCAAACC GGGCCGACAC ATGGTTGACG ATGTCCTTTT

951 CTACAATGAA GTTAATGAAA GTTCTGAAAA TAGTGATTAC TTTCTGACAT

1001 TGATAGGATT TAGGAAACCT CTGGATAAAT AGCTTAAGCA TGGCTGTTTA

1051 TGTTTTTGCT ATAGACAAAA AGCAGCAGCA TGTACATTGT ATTTGGACAC

1101 AAGCCTGCCT CGGTTAATAT ATTGAACTAT TGGACCACTA GGGTTAGTAG

1151 GGAGCGGTCT GTACACTTTC TGATTCAGCA TTCAGAAACA TTCTAGGTGG

1201 ACTCTGTAGC TTTCAGTTTT GTAAAGTTAT CGGAAAAACA TCGGGAGGGT

1251 TTGGCCATCA TATGTGAGCT TTGTGTTTCA ATGCCAGTTA CTCAGGATTA

1301 GTAAATTAAT GACTGTCCAG AGGACTTCAG GGTCACCAAG CTGCTGCACC

1351 TGCCATTGGC TGACTCTCCC CGGCTATCTG TGGCTGAGAT GGTGCTGCTT

1401 AGGTCACGCA GAGCATGAGC TGCTGCTGAA AGGGCACAGG AGATGGCCCT

1451 TGGGCTTCTC ATCCCAGGAT GCCTGCCCTG CCCACCAATC CATGAGAAGA

1501 TATGTATGAT TTCAGTAGGC CCTGGATCAG CTTGTCACCT CTGGTTTCCT

1551 GTTTGCTTTC CACTCACTCA GCTGGAGTTT CATTTCCAGA CTAAAGTCTT

1601 CATCATTGGC TTCAGAAACA GCATTCATCT GTGGCTGTGC TGATGTAGTA

1651 CACCAAGAAC AACTGGGCTC TTCTCTGTCA CTTTCAGTGG GCTACCTTCC

1701 CTCACCTCTC CAAGCAGCAT GAAAGAATTC TTTACATTTT TAATCTCTTT

1751 TTTGTTTTTC CCTGAAAGTA TGCTTTGGTG CTTAAAGAGA GAAGTCACAA

1801 AAGTATACTA CTGAGTTTCC TGGAGATGAA ATCCTGTTGT CCCTAGCTAT

1851 GTGAATGAGC ACAGGGATCC CTGATGCCAT TATTTTGTAT ATTCATACGG

1901 CACACACTTA CTGAGGGCCT TCTGTGTGCC CTAGGGGATT GAGCACAGTG

1951 ACATATCAGG GCAGGTAGAA ACAGATGGAG AGCTGATGCG GGCTGTCTTA

2001 GAGCAGCTGC CCCAGGAGGC CCCTGTGGAT GGATGTTGGG CAGGAGCCCT

2051 GAGACGTTAG GGGCATATAA CTAAAGGACA TAGCAGGAGT TATAGGAGGA

2101 GCTGATCCCT GAGGGAAACA ATGAAGACGG AGAAGATGGG GCTAAAGTTT

2151 GAATTGTGGG GACATTAATC ACGGTGATTC TTAAAACTTT GCTGTTGATG

2201 ATTTTAAATG GAGAAAATGA GTACGTAAGA TGTTATTTCC CAGTTCAGTA

2251 TATAGGTTGC CCACAAAGTA TTTTCCTACC ATGAATGGTC ATATATACTT

2301 GTTGTAGAAT ACCAGGGACA GCAGAGATGG TGGGGTAGTT ACTTCCTTTT

2351 CTTACAGCCC AAGAACTTTG GTGTCCAGGA GATTGACCAA TTTAGCCACT

2401 GAGCATTTAA TACAACACAG GGCTACCCAG ATCCCACTGT CCTGATTTGC

2451 CCTGAAAGCC AAAGGAGTCA GGAGAAGGTG AGTGGGGTGA ATATATTAAT

2501 CCTGAGAGTT GAACAGAGCA AAAATCCCTA TTACTTTTGT ACTTAAAACA 2551 TCTCTGCCAC ATGTGCTCAC TCTTTATATT CTGTTTAGGT GGTTTATATG

2601 TGCACATCCC ATCCTATGCC TGCAGTTAGC CAACTCAGGG TTTATATTGC

2651 CTCCTTTCTT TTTTTCTTTT TTTTAAGAGA TGGGGTCTCG

2701 TTCTGTCATG CAGACTGGAG TGCAGTGGTG TGATCACAGC TCATTGTAAC

2751 CTCCAACGCC TGGACTGAAG TGATCCTCCT GCCTTGGCCT CTCTGGTAGC

2801 TGGGACTACA GGTGCATGCC ACCACACCCA CCTAATTTTT TTTATTTTTA

2851 TTTTTTGTAG AGACAGTCTC ACTATCTTGC TCGGGCTGGT CCTGAACTCC

2901 TGGGCTCAAG TTATCTTGCT GCCTCAGCCT CCCATGGGTA ATCTTTATTT

2951 CCTTTTTTTT TTTTTTTTGG AGATGGAGTT TCGCTCTTGT CGCCCAGGCT

3001 GGAGTGCAAT GGCACGATCT TGGCTCACTG CAGTCTCCAC CTCCTGGGTT

3051 CAGGTGATTC TCCATCCTCG GCCTACTGAG TAGCTGAGAT TACAGGCAAC

3101 TGCCACCATG CGCGGCTAAT TTGTGTATTT TTTTTTAGTA AGAGATGGGG

3151 TTTCGCCATG TTGGCCGGAC TGGTCTTAGA CTCCTGACCT CAAGCGACCT

3201 GCCTGCCTTG GCCTCCCAAA GTGCTGGGAT TACAGGCATG AGCCGCTATG

3251 CCTCGTCGCT GATTTTTATT TCTTATTTTT TTTTTAGAGA TGGGGGTCTC

3301 ACTATGCTGC TCAGGCTGAT CTCAAACTCC TGGCCTCAAG TGATCCTCCC

3351 ACCTTAGCCT CCCAAGTTGC TGGGATTATA AGTGTGAGCC ACTATCCCTA

3401 CCTCACTATT ACCTTCTTTG CTTCTCTTGT TTTCTTTTGT TCTAAGTCAA

3451 ACCCATCACA ATCTTTTCTT GTCCTTCCAG GTGTTTTCCA GTGCTGTGCC

3501 CTGGATGTGC TCTCTTTCTC TTAGAGCCCA GAGAACTTGC TTTTCCCCCT

3551 TATATATGAC CCTTAACTTT TTCTAACACA TTATTAAGGG CCTGTGTCTA

3601 TCAGCTGGGG GCACTTCTTG AAGGGAGGGC CTTTGTGTGG TCTGTTTCTA

3651 GTGACTTCCA GCTTTAACCC AGAGCCTCAT GATTGCTGGG TGCCCATAGC

3701 CTTTTTGCTG AATGGAGGCA CTCAGTCTCC TTGGGAAGAG AGAATCCATG

3751 ATAGACCCAC TTGGGAGCTC CCCACTTCAG GGGCCTACAC ACTGGTAATG

3801 CAACAGAATG CCCAAGAGTG ACCTCATAAA GCAAGGATTC CCTTCGTGGC

3851 CCCTTCTCTG CTGCCTCTCA GAATCCAGAC GCTAAGGAAA ATCCCTAAGC

3901 AGAGATTTTC TGTTGGATGC TAAAAGCAAG GAATAAAAGT TGAAAATTTG

3951 GAAAATGTCT CAACACCGTC ACCAGCGCCA CTCGAGAGTC ATTTCTAGTT

4001 CACCAGTTGA CACTACATCG GTGGGATTTT GCCCAACATT CAAGAAATTT

4051 AAGTAAATAT TATCTATCTC CATTGCCTGT TAAGAAATGT GCTAGTAGAA

4101 GTGTGAGGGC AGGGTGTCAG TGTTCTCTCA GCCTCTTCCC TCAGATACTC

4151 GTCTGCTTAC CAAAATAAGT TGCATGTCCT TGACAATCTG GTTTCTATGA

4201 TTGGTGAGGC TGGCATGCTA TTACCTTTAT GTGCCCTGTA GACTTGAATG

4251 ACCAGTTTGA CCAGTTTGAC TGTTAGATAA TCAGAAGGCT TTTCTCTTTT

4301 TTTATAATAG ACCCCATCTC AAATCAGATA ATGAAAATTA CATATCTTGA

4351 TATATTAGAA AAGTATATAC ATTCTGGCTG GGCACGGTGG CTCACGCCTG

4401 TAATCCCTGC ACTTTGAGAG GCTGGGGCGG ATCACTTGAG GTCAGGAGTT

4451 TGAGACCGGC CTGGCCAGCG TGGCGAAACC CCATCTCTAC TAAAAATACA

4501 CAGATTAGCC CGGAGTGATG GTGTGCACCT GTTGTCCCAG CTACTCAGGA

4551 TGCTGAGGCA GGAGAATCCC TTTAACCTGG GGGGCGAAGG TTGCAGTGAG

4601 CCAGGATTGC ACCACTGCAC TCCAGCCTGG GTGACGGAAC GGGACTCTGT

4651 CTCAGAAAAA AAAAAAAAGA AGAGGAAAAA GAAAAATATA TATTCTATAT

4701 TTTTTTAACT TATGAGAATG TGTTCATTTC ATTTGTAACA TATAATGGGA

4751 AACAGTAATA CGTACTCTGA GAAAAATTGC AAAGCACAGA TAAATGGAAA

4801 TAAACAGGAA AAAGAATCAC CTATAACCTC ACCATCCATA GACAGACACT

4851 GTTAAAATTT TGGCATATTT CCTGCTGATT TTTTCTACTG CTGATTTTTG

4901 CACAGGTGAG ATAATTTTGA ACAGAGAATT TTGTATCTTT GGTTTTTGTG

4951 TTTCGCTGCA CACAAAAACA AAAGATATAA AAATGGATCA TAAACATTTT

5001 TCTAAATCCT GAAAAGTGCA TAGACATATT TTAGTGCCTG TATTTCACAA

5051 GATGGACATA CCATAATTTA CTTACACAGT CCTTTTTGTT AGATGTTTAA

5101 GTTGTTTTCA AGCTTCTCAG TGCTGGAAAA AATACTGAGA TAGACATGTT

5151 TAGTTGAAGT TATTTCATTT CAGGTTATAT TATCTTGGGT CAGAGAATGA

5201 ATGGTTCTCA GGCTTTTCAA AAGAGCTGGT CAGTTTTTAT GCCTCTGGCA

5251 GTTTTTGAGA GTGCTCAATC ATACTACACT GTTGCCAGCA TTAGATCTTA

5301 TCACATTTAA GTCATTGCTA ATTTTATAAA CAAAAACAAT GGTTTTACTT

5351 TGCATCTCCC TGATTGGTGT TGCTGTAGAA CATATTTGGA GAAGTTTGTT

5401 TGTCTTTGGT GTTTATTCCA TGAATAGATT GTGTGCCCAT TTTCTCTTGG

5451 GGTATTCAGT TTTTTATTAC TGATGTGAGC ATGTGTATGG GTGATTATTT

5501 GATGATTATC AGTTTTGCTT AGTAGACTGG CAATATTTAG TCTTGCTGTC

5551 ACTGTGTTCC CAGTGCCAAC TAGATTGCTT GATATGTAGT TGCCACTCAA

5601 TAAAGATTTG TTGAGTCAAT GAAAAAAAAA AAAAAAAAAA A

BLAST Results

Entry AC004764 from database EMBL:

Homo sapiens chromosome 5, PI clone 255g5 (LBNL H61), complete sequence.

Score = 11057, P = 0.0e+00, identities = 2217/2224 Bp 428-5625 of cDNA == Bp 2912-8107 of AC004764

Entry HSAC1555 from database EMBL:

Homo sapiens (subclone l_d8 from BAC H75) DNA sequence, complete sequence .

Score = 575, P = 5.1e-30, identities = 115/115 Bp -240- 430 of cDNA == HSAC1555 splice pattern Medlme entries

93186787:

Phenylalanine hydroxylase-stimulating protem/pterm-4 alpha-carbinolamine dehydratase from rat and human liver. Purification, characterization, and complete ammo acid sequence .

93101632:

Identity of 4a-carbιnolamιne dehydratase, a component of the phenylalanine hydroxylation system, and DCoH, a transregulator of homeodomain proteins.

95242099:

Crystal structure of DCoH, a bifunctional, protein-binding transcriptional coactivator

Peptide information for frame 3

ORF from 21 bp to 410 bp; peptide length: 130 Category: strong similarity to known protein

1 MAAVLGALGA TRRLLAALRG QSLGLAAMSS GTHRLIAEER NQAILDLKAA 51 GWSELSERDA IYKEFSFHNF NQAFGFMSRV ALQAEKMNHH PEWFNVYNKV 101 QITLTSHDCG ELTKKDVKLA KFIEKAAASV

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfkd2_46kl9, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphfkd2_46kl9, frame 3

Report for DKFZphfkd2_46kl9.3

[LENGTH] 130 [MW] 14377.56 [pi] 9.17 [HOMOL] PIR:A47189 pterιn-4-alpha-carbmolamιne dehydratase (EC 4.2.1.96) - rat 4e-34

[FUNCAT] 01.07.99 other vitamin, cofactor, and prosthetic group activities [S. cerevisiae, YHL018w] 5e-04

[SCOP] dldchg_ 4.38.1.1.1 Pterιn-4a-carbmolamιne dehydratas 4e-50

[EC] 4.2.1.96 Tetrahydrobiopterin dehydratase 6e-34

[PIRKW] nucleus 6e-34

[PIRKW] carbon-oxygen lyase 6e-34

[PIRKW] homotetramer 6e-34

[PIRKW] hydro-lyase 6e-34

[PIRKW] cytosol 6e-34

[PIRKW] acetylated amino end 6e-34

[PIRKW] homodimer 6e-34

[SUPFAM] pterιn-4-alpha-carbιnolamme dehydratase 6e-34

[PROSITE] MYRISTYL 2

[PROSITE] CK2_PHOSPHO_ΞITE 3

[PROSITE] PKC_PHOSPHO_SITE 4

[KW] Alpha_Beta

[KW] 3D

[KW] LOW COMPLEXITY 14.62 %

SEQ MAAVLGALGATRRLLAALRGQSLGLAAMSSGTHRLIAEERNQAILDLKAAGWSELSERDA

SEG . xxxxxxxxxxxxxxxxxxx ldchB CCCCHHHHHHHHHHHHHHCCEEECCCCE

SEQ IYKEFSFHNFNQAFGFMSRVALQAEKMNHHPEWFNVYNKVQITLTSHDCGELTKKDVKLA

SEG ldchB EEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEETTTEEEEEECBTTTTBTCCHHHHHH

SEQ KFIEKAAASV SEG ldchB HHHHHHHHHH

Prosite for DKFZphfkd2_46kl9.3

PS00005 11->14 PKC_PHOSPHO_SITE PDOC00005 PS00005 32->35 PKC_PHOSPHO_SITE PDOC00005 PS00005 56->59 PKC_PHOSPHO_SITE PDOC00005 PS00005 113->116 PKC_PHOSPHO_SITE PDOC00005 PS00006 56->60 CK2_PHOSPHO_SITE PDOC00006 PS00006 105->109 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 113->117 CK2_PHOSPHO_SITE PDOC00006 PS00008 6->12 MYRISTYL PDOC00008 PS00008 20->26 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfkd2_46kl9.3)

DKFZph kd2 46m4

group: signal transduction

DKFZphfkd2_46m4.3 encodes a novel 198 amino acid putative GTP-binding protein related to the SAR-1 family of Ras superfamily members.

SARI proteins are involved in vesicular transport between the endoplasmic reticulum and the Golgi apparatus .

The new protein can find clinical application in modulating the transport of vesicles to the Golgi Apparatus, thus enabling post-translational modifications of the vesicles contents. Blocking of the molecule is expected to result modulation/blocking of secretory pathways.

nearly identical to mouse GTP-bindmg protein complete cDNA, complete eds, EST hits

Sequenced by MediGenomix

Locus: /map="438.9 cR from top of ChrlO linkage group"

Insert length: 2996 bp

Poly A stretch at pos. 2969, polyadenylation signal at pos. 2958

1 ACATCCGGCG AGTAGCTGGC GGTCCCGGGT GCTGCTGGTT AGTGTGCTCT

51 GAGGGAGGGT CCGAGCCAGC CGCTGTTTTG CCGGAGGAGC CCCTCAGGCC

101 GTAGTAAGCA TTAATAATGT CTTTCATCTT TGAGTGGATC TACAATGGCT

151 TCAGCAGTGT GCTCCAGTTC CTAGGACTGT ACAAGAAATC TGGAAAACTT

201 GTATTCTTAG GTTTGGATAA TGCAGGCAAA ACCACTCTTC TTCACATGCT

251 CAAAGATGAC AGATTGGGCC AACATGTTCC AACACTACAT CCGACATCAG

301 AAGAGCTAAC AATTGCTGGA ATGACCTTTA CAACTTTTGA TCTTGGTGGG

351 CACGAGCAAG CACGTCGCGT TTGGAAAAAT TATCTCCCAG CAATTAATGG

401 GATTGTCTTT CTGGTGGACT GTGCAGATCA TTCTCGCCTC GTGGAATCCA

451 AAGTTGAGCT TAATGCTTTA ATGACTGATG AAACAATATC CAATGTGCCA

501 ATCCTTATCT TGGGTAACAA AATTGACAGA ACAGATGCAA TCAGTGAAGA

551 AAAACTCCGT GAGATATTTG GGCTTTATGG ACAGACCACA GGAAAGGGGA

601 ATGTGACCCT GAAGGAGCTG AATGCTCGCC CCATGGAAGT GTTCATGTGC

651 AGTGTGCTCA AGAGGCAAGG TTACGGCGAG GGTTTCCGCT GGCTCTCCCA

701 GTATATTGAC TGATGTTTGG ACGGTGAAAA TAAAAGAGTT TTACTTCTCT

751 GGACTGATCC TATTCACAGC TTCCTCATGA ACTTTTCTAA TAGAACAAGG

801 ATAGCTCTCC AACCATGTCT GGCGTTGAGA AGCCAAGAGT CTCTGTCAAC

851 TCTCTCATTG CCCAGTGGTG ACATGTGCTC TTCTCCACAC TGTTGGGAGG

901 TAATGCTGCC CCACGTGCTG GTGCAGGTCA GTATCCTGGG ACTTGGAAGC

951 TGGCAGGATT TGCCGGGTAA AGCTGTATGC CATCATGGGG CACCTGAAAA

1001 GAAAAACACG TCTCACCACT GTGGTTGATT CAAAAGAAAG TGATTCTATT

1051 TTTTAAAGAA AGCGTTGTTA ATGTAATTGG TATCCCTCCT AACTTTTTGA

1101 GTTCACAATT TACTTGGTCC AGAGTTTTCT ATTCTTTTTT TTTTTTTAAA

1151 CTAATGAATG ACATTTAGAT ACTTCATAAA ATTATGAACA GATATGGAGG

1201 CCAGAGCTCA TTTGGGTAAA CTTACTCCTG CTGAGTTAGC AGGTTGGTGA

1251 GAGAAGCTCC CCTGAGCTCA CCTGTCTCTC TGACTGCCTT GGAGTAGGTG

1301 GCATAACCTT GTGCACAGAG AACTAGAAAA GGGGCAGAAC CCCGGCCTTG

1351 CAGTTGTGGC AGGTTTCCAC TGTGGTAAGC TAGGTTCATT CCTCATCAAG

1401 GAATGTGTAG CAGATTGTTC ACTGTGGAGG AGGTAATTAT AGAATGGGTT

1451 ATTGTTGTTA TTCTTACTCA TGAAGTTACA GATTTTAGCC AGTCTTTGCT

1501 TTTATACTTT TGTGAAATTT AATTTCTCTC TATAGCACCT TCCTTTTTCG

1551 TTTTCAGTTA TCAAAAGTGA CTTTGACCTC ATAAGAGAGT TGAGAACATC

1601 TCTCGTGTCA CATACTGCAG GTGCATCAGT TACTTTTGCA CAGATTCTAG

1651 GGGGACATTT TTCTGAATAG GAAGACAGGA CAAAGTTAAC AGCTTAAGGG

1701 CTCTTAATTC TGTGAGTTGA GGACTTAAAA GTATTGTAGC ATTTGTTTGG

1751 ATCCATGAAA AATGTATTCA GTGGGCTTTA AAATTTCCAT TTGCAGAATT

1801 TGGTCTCTCA GGCTGTTTGG GAGCTCTTTT TTTTACATTT TTTCTCCTTT

1851 GACACCTATT TTATTGGTGT TTAAAGTAAA GGTTAACATC TGTAGCTTTT

1901 CCAGGTTTTT TTTTTTTTTT TTGATATGAA ATTGTCTTTC TCCATTGCAG

1951 AAATAAGCTA GGGAAACACT AACCCAAAAA CTTTCTGTAG AGCTGTTCCT

2001 TTGGAGGCAG CATCACTTAT TGGCAGTAAA GACTCAGTAT AAAAGCACCA

2051 GCATCCCTAC TTGGGTGATG GGGATTAATT TTATAGCATT CCATTTTCCT

2101 AGTGCCACAT GTGAAATTGG ATTTTGATGA TCTTAATCTA TATTCTACCC

2151 TTATAATAAA AGATCAAAAG ATATATCTCC TATGAACAGA TTGGAGATAG

2201 GAGATGAAAA GTTGGGAGGA TGCCTTTATT CTAATGTGAG GGTAGGGAAA

2251 ATGTGGATAA CATTACTGGG GTGAAGGAGG CATTGTTCTT TAGTTGGAGT

2301 TCTCATTTTT ATTCTCCAGT ACTGACTTGT GGGGAAAGCA TACTTTTTCA

2351 CTGCCAGGTA CTGAATGCAG AGGCTCAGTG AAGTATATAT GTGGGAAGTG

2401 CATGCATTTC GTTTATTAGC AAACATAGCT GGATTAAGAC GAAGTTGTTG

2451 GTTTGGAAAG GGGTTAAAGC CTTAAGTGAA CAAATCTAGC TAACAGTGAA

2501 TGAACTAGGT AATATAACTT GCATATTTTT AATTTCCTTT GGTTAAAGGT

2551 CCCCCATACT TCTCTGTTCG GAGACATGAG AAGTATGATT ACTTCAGTGT 2601 TAGTTTTCTT AATTTTTTTT TTCCCCTATT TGTCCCTTGT CACTTTGTTG

2651 CAAGCTAGAA ATCTGTGGGT TATACATAGG GCAGCTCTTT GCGAAAGTGG

2701 TTTATTCCAC TGGAGAAAGG GGATTGAAAA TCAGTTAGAA CCAATGTATT

2751 TCTTGCCCCA CGGAACACTA TTCCTATAAG ATAGCTGAAA GAAGCTGCTG

2801 TGAGGAGCTC AGCTCCAACA CAGGATCAGC ACCTTGTATA GGAATTCCCA

2851 TGAATTATGA CTTCTCATTC TGTTTTATCA GAGTGCATAT ATGTCCTACT

2901 TCAGGAAAAG TAAAACAGTC ATTTACGAAA GAAAGTCAAT CTGTATCCTA

2951 AGCATTTTAA TAAAAAGTTA AAACAAAAAA AAAAAAAAAA AAAAAA

BLAST Results

Entry HS679348 from database EMBL: human STS WI-16722.

Length = 265

Minus Strand HSPs:

Score = 1242 (186.4 bits), Expect = 2.8e-50, P = 2.8e-50

Identities = 260/265 (98%)

Medline entries

94085558:

Molecular analysis of SARl-related cDNAs from a mouse pituitary cell line.

Peptide information for frame 3

ORF from 117 bp to 710 bp; peptide length: 196 Category: strong similarity to known protein

1 MSFIFEWIYN GFSSVLQFLG LYKKSGKLVF LGLDNAGKTT LLHMLKDDRL

51 GQHVPTLHPT SEELTIAGMT FTTFDLGGHE QARRVWKNYL PAINGIVFLV

101 DCADHSRLVE SKVELNALMT DETISNVPIL ILGNKIDRTD AISEEKLREI

151 FGLYGQTTGK GNVTLKELNA RPMEVFMCSV LKRQGYGEGF RWLSQYID

BLASTP hits

Entry S39543 from database PIR:

GTP-binding protein - mouse

Length = 198

Score = 1029 (362.2 bits), Expect = 5.1e-104, P = 5.1e-104

Identities = 197/198 (99%), Positives = 198/198 (100%)

Entry SARA_MOUSE from database SWISSPROT:

GTP-BINDING PROTEIN SARA.

Length = 198

Score = 1012 (356.2 bits), Expect = 3.2e-102, P = 3.2e-102

Identities = 195/198 (98%), Positives = 196/198 (98%)

Entry CEZK180_4 from database TREMBL: gene: "ZK180.4"; Caenorhabditis elegans cosmid ZK180.

Length = 193

Score = 679 (239.0 bits), Expect = 6.3e-67, P = 6.3e-67

Identities = 125/197 (63%), Positives = 161/197 (81%)

Alert BLASTP hits for DKFZphfkd2_46m4, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphf d2_46m4, frame 3

Report for DKFZphfkd2_46m4.3

[LENGTH] 198

[MW] 22367.00

[pi] 6.21

[HOMOL] PIR:S39543 GTP-binding protein - mouse le-112 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YPL218w] le-58

[FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae,

YPL218w] le-58

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YOR094w] 2e-23

[FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YPL051w] 4e-22

[FUNCAT] 30.08 organization of golgi [S. cerevisiae, YDL192w] 3e-20

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YBR164c] 3e-19

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YMR138w] 2e-09

[FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YMR138w] 2e-09

[FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YHR168w] 7e-05

[FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YHR005c] le-04

[FUNCAT] 30.07 organization of endoplasmatic reticulum [S. cerevisiae, YKL154w] le-04

[FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins

[S cerevisiae, YHR005c] le-04 [FUNCAT] 10.05.07 g-protems [S. cerevisiae, YHR005c] le-04 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YKL154w] le-04 [FUNCAT] 08.19 cellular import [S. cerevisiae, YMLOOlw] 3e-04 [BLOCKS] BL00395A Alanine racemase pyridoxal-phosphate attachment site proteins [BLOCKS] BL01019B ADP-ribosylation factors family proteins [BLOCKS] BL01019A ADP-ribosylation factors family proteins [BLOCKS] BL01020D SARI family proteins [BLOCKS] BL01020C SARI family proteins [BLOCKS] BL01020B SARI family proteins [BLOCKS] BL01020A SARI family proteins [SCOP] dlplj_ 3.25.1.3.1 cH-p21 Ras protein [human (Homo sapiens) 7e-36 [SCOP] dlguaa_ 3.25.1.3.10 RaplA [Human (Homo sapiens) 8e-40 [SCOP] dlrrf 3.25.1.3.5 ADP-ribosylation factor 1 (ARFl) [rat (Rattu 2e-55 [SCOP] dlhurb_ 3.25.1.3.4 ADP-ribosylation factor 1 (ARFl) [human (Hom le-58 [SCOP] dlgota2 3.25.1.3.3 (1-54,171-326) Transduem (alpha subunit) [ra 2e-33 [SCOP] dltadb2 3.25.1.3.2 (1-30,152-316) Transduem (alpha subunit 6e-36 [PIRKW] glycoprotein 4e-19 [PIRKW] monomer le-16 [PIRKW] P-loop 3e-64 [PIRKW] lipoprotein 4e-19 [PIRKW] GTP binding 3e-64 [SUPFAM] ADP-ribosylation factor 5e-22 [PROSITE] ATP_GTP_A 1 [PROSITE] MYRISTYL 3 [PROSITE] SARI 1 [PROSITE] CK2_PHOSPHO_SITE 4 [PROSITE] PKC_PHOSPHO_SITE 3 [PROSITE] ASNJ3LYCOSYLATION 1 [PFAM] ADP-ribosylation factors Arf family) (contains ATP/GTP binding P-loop) [KW] Alpha_Beta [KW] 3D

SEQ MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPT lhurA TTTTTCCCCEEEEEETTTTCHHHHHHHHCCCCEEEEEEETTEE

SEQ ΞEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMT lhurA EEEEEETTEEEEEEETTTTTTTCCCHHHHHHCEEEEEEEEETTTTTHHHHHHHHHHHHHH

SEQ DETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNARPMEVFMCSV lhurA TTTTTTTEEEEEEETTTTTTTCCHHHHHHHHCGG

SEQ LKRQGYGEGFRWLΞQYID lhurA

Prosite for DKFZphfkd2_46m4.3

PS00001 162->166 ASN_GLYCOSYLATION PDOC00001 PS00005 25->28 PKC_PHOSPHO_SITE PDOC00005 PS00005 158->161 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 164->167 PKC_PHOSPHO_SITE PDOC00005 PS00006 60->64 CK2_PHOSPHO_SITE PDOC00006 PS00006 72->76 CK2_PHOΞPHO_SITE PDOC00006 PS00006 111->115 CK2_PHOSPHO_SITE PDOC00006 PS00006 164->168 CK2_PHOSPHO_SITE PDOC00006 PS00008 32->38 MYRISTYL PDOC00008 PS00008 68->74 MYRISTYL PDOC00008 PS00008 155->161 MYRISTYL PDOC00008 PS00017 32->40 A P_GTP_A PDOC00017 PS01020 171->197 SARI PDOC00782 Pfam for DKFZphfkd2_46m4.3

HMM_NAME ADP-ribosylation factors (Arf family) (contains ATP/GTP binding P-loop)

HMM *GMgWfsIFrkMWGlWNKEMRILMLGLDNAGKTTILYMLKlgEIVTTIPT

++ FS++++++GL++K++++++LGLDNAGKTT+L+MLK++++ +++PT Query 9 -YNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPT 56

HMM IGFNVETVeYKNIKFNVWDVGGQdsIRPYWRHYYpNTDGIIWVVDSaDRD

+++++E++++ +++F+++D+GG++++R++W++Y P+++GI+++VD+AD++ Query 57 LHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHS 106

HMM RMeEaKqELHaMLNEEELrDAPlLIFANKQDLPgAMSesEIREaLGLHel

R+ E+K+EL+A++++E ++++P+LI++NK+D+ +A+SE+++RE+ GL+ + Query 107 RLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQ 156

HMM RCn RPWYIQMCCAVtGEGLYEGMDWLSNYInkRkK*

+++ RP++++MC++++++G++EG++WLS+YI Query 157 TTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYI 197

DKFZphfkd2_47a4

group: transcription factor

DKFZphfkd2_47a4.1 encodes a novel 280 ammo acid protein with similarity to zinc fmger proteins.

The new protein is a putative transcription factor with one C2H2 zinc fingers.

The new protein can find application m modulating/blocking the expression of genes controlled by this transcription factor. similarity to C. elegans F46B6.7 potential frame shift at 1092, will be checked see BLASTX

Sequenced by MediGenomix

Locus: map="7q31"

Insert length: 1756 bp

Poly A stretch at pos. 1737, no polyadenylation signal found

1 CCCTTTTCTT TTCTGCCGGG TAATGGCTGC TTCCAAGACC CAGGGGGCTG 51 TCGCCCGAAT GCAGGAAGAC CGTGATGGGA GCTGCAGCAC AGTCGGGGGT

101 GTAGGTTATG GGGTAAGGAT TGTATCCTGG AGCCGCTTTC CCTGCCAGAA

151 AGTCCAGGTG GCACCACCAC TTTAGAAGGT TCTCCATCTG TGCCTTGTAT

201 TTTCTGTGAA GAACATTTTC CTGTGGCTGA ACAAGACAAA CTTCTGAAGC

251 ACATGATTAT TGAGCATAAG ATTGTCATAG CTGATGTCAA GTTGGTTGCT

301 GATTTCCAAA GGTACATTTT ATATTGGAGG AAAAGGTTCA CTGAACAGCC

351 CATCACAGAT TTTTGTAGTG TAATAAGAAT TAATTCCACT GCTCCATTTG

401 AAGAACAAGA GAATTATTTT TTGTTATGTG ACGTTTTACC AGAAGATAGA

451 ATTCTTAGAG AAGAGCTTCA GAAACAGAGA CTGAGAGAAA TTCTGGAACA

501 ACAGCAGCAA GAACGAAATG ATAACAATTT TCATGGCGTT TGTATGTTTT

551 GCAATGAAGA ATTCCTTGGA AACAGATCTG TTATTTTGAA CCACATGGCC

601 AGAGAACATG CTTTCAACAT TGGATTGCCA GACAACATTG TAAACTGCAA

651 TGAATTTTTG TGTACATTAC AGAAAAAGCT TGACAATTTG CAGTGCTTGT

701 ACTGTGAGAA GACCTTCAGG GGCAAAAATA CACTTAAAGA TCACATGAGG

751 AAAAAACAGC ATCGTAAGAT TAATCCTAAG AACAGAGAAT ATGACAGATT

801 TTATGTCATC AATTATTTGG AACTTGGAAA ATCGTGGGAG GAAGTTCAGT

851 TGGAAGATGA TCGGGAGTTG CTGGACCATC AGGAAGATGA CTGGTCTGAT

901 TGGGAAGAAC ACCCTGCCTC TGCAGTCTGC TTATTTTGTG AAAAGCAAGC

951 AGAAACAATT GAGAAGTTGT ATGTCCACAT GGAGGATGCA CACGAATTTG 1001 ATCTTCTCAA AATAAAGTCA GAACTTGGAT TAAATTTCTA TCAGCAAGTG 1051 AAACTGGTCA ATTTTATTCG GAGGCAAGTT CACCAATGCA GATGATGGCT 1101 GCCATGTGAA GTTCAAATCC AAAGCAGACT TAAGAACTCA CATGGAAGAA 1151 ACTAAACACA CTTCGCTGCT CCCCGATAGA AAGACGTGGG ATCAACTGGA 1201 GTATTATTTT CCAACCTATG AAAATGACAC TCTCCTGTGT ACACTATCTG 1251 ACAGTGAAAG TGACCTGACA GCTCAGGAAC AAAATGAAAA TGTTCCCATC 1301 ATCAGTGAAG ATACATCTAA ACTGTATGCT TTGAAACAAA GCAGTATTTT 1351 GAACCAGTTG CTACTATAAG AGTACTTGAA AACCTAGAAG AAACTACCAC 1401 AGAAGCAATT TTTCATGTTT TTCTCCTATG AGACAGATAT GAAAGAACAA 1451 TTTAAATTTG AACATCAACA AAAGATTGGT CCTTGGTGAA ATAAACTTTT 1501 CAAAAATGAA TGTTCTTTTC AAAAAATAAA GTAGAAAAAT GCACTTACTA 1551 AGAACATGAA AAAAAAATGA AGTAGGAAAA TAAGATGAAG ACTTTGTATT 1601 TTGGCTGTAA AGTTTTATTG TGTGATCATC TTAAATTATC TCACTTCATT 1651 AAACTCATAA TTATATA AG AAGTA ATGT CAATTACAAA GAAATGAAAT 1701 GTTCAAATTA TTTATAAACC TGATTTTTCA ATCAGCGAAA AAAAAAAAAA 1751 AAAAAA

BLAST Results

Entry AC004112 from database EMBL:

Homo sapiens BAC clone RG313E03 from 7q31, complete sequence.

Score = 2660, P = 3.0e-241, identities = 534/535

> 10 exons

Entry AC004111 from database EMBL:

Homo sapiens BAC clone RG103H13 from 7q31, complete sequence.

Score = 598, P = 5.8e-17, identities = 128/137

1 exon

Medline entries No Medline entry

Peptide information for frame 1

ORF from 253 bp to 1092 bp; peptide length: 280 Category: similarity to unknown protein

1 MIIEHKIVIA DVKLVADFQR YILYWRKRFT EQPITDFCSV IRINSTAPFE 51 EQENYFLLCD VLPEDRILRE ELQKQRLREI LEQQQQERND NNFHGVCMFC 101 NEEFLGNRSV ILNHMAREHA FNIGLPDNIV NCNEFLCTLQ KKLDNLQCLY 151 CEKTFRGKNT LKDHMRKKQH RKINPKNREY DRFYVINYLE LGKSWEEVQL 201 EDDRELLDHQ EDDWSDWEEH PASAVCLFCE KQAETIEKLY VHMEDAHEFD 251 LLKIKSELGL NFYQQVKLVN FIRRQVHQCR

BLASTP hits

Entry CEF46B6_6 from database TREMBLNEW: product: "F46B6.7"; Caenorhabditis elegans cosmid F46B6

>TREMBL:CEF46B6_6 product: "F46B6.7"; Caenorhabditis elegans cosmid

F46B6

Score = 630, P = l.le-61, identities = 123/289, positives = 183/289

Entry AF059531_1 from database TREMBLNEW: gene: "PRMT3"; product: "protein arginine N-methyltransferase 3"; Homo sapiens protein arginine N-methyltransferase 3 (PRMT3) mRNA, partial eds. >TREMBL:AF059531_1 gene: "PRMT3"; product: "protein arginine

N-methyltransferase 3"; Homo sapiens protein arginine

N-methyltransferase 3 (PRMT3) mRNA, partial eds.

Score = 120, P = 1.5e-04, identities = 23/78, positives = 42/78

Entry YB9M_YEAST from database SWISSPROT:

34.7 KD PROTEIN IN SHM1-MRPL37 INTERGENIC REGION.

Score = 112, P = 4.6e-04, identities = 43/165, positives = 71/165

Alert BLASTP hits for DKFZphfkd2_47a4, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphfkd2_47a4, frame 1

Report for DKFZphfkd2_47a4.1

[LENGTH] 280 [MW] 33921.94 [pi] 5.63 [HOMOL] TREMBL :CEF46B6_5 gene: "F46B6.7"; Caenorhabditis elegans cosmid F46B6 le-56

[BLOCKS] BL01032B Protein phosphatase 2C proteins

[BLOCKS] BL00028 Zinc finger, C2H2 type, domain proteins

[PROSITE] MYRISTYL 1

[PROSITE] ZINC_FINGER_C2H2 1

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 3

[PROSITE] TYR_PHOSPHO_SI E 2

[PROSITE] PKC_PHOSPHO_SITE 2

[PROSITE] ASN_GLYCOSYLATION 2

[PFAM] Z nc finger, C2H2 type

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 8.21 %

SEQ MIIEHKIVIADVKLVADFQRYILYWRKRFTEQPITDFCSVIRINSTAPFEEQENYFLLCD SEG PRD cccccceeehhhhhhhhhhhhhhhhhhhhhhhcccceeeeeeccccccchhhhheeeecc

SEQ VLPEDRILREELQKQRLREILEQQQQERNDNNFHGVCMFCNEEFLGNRSVILNHMAREHA SEG xxxxxxxxxxxxxxxxxxxxxxx

PRD ccccchhhhhhhhhhhhhhhhhhhhhhhhcccceeeeeeccccccccceeeehhhhhhhh

SEQ FNIGLPDNIVNCNEFLCTLQKKLDNLQCLYCEKTFRGKNTLKDHMRKKQHRKINPKNREY SEG PRD hcccccccccchhhhhhhhhhhhhhhhheeecccccccchhhhhhhhhhhcccccccccc

SEQ DRFYVINYLELGKSWEEVQLEDDRELLDHQEDDWSDWEEHPASAVCLFCEKQAETIEKLY SEG PRD ceeeeeeeeccccchhhhhhhhcchhhhhhcccccccccccccccchhhhhhhhhhhhhh

SEQ VHMEDAHEFDLLKIKSELGLNFYQQVKLVNFIRRQVHQCR SEG PRD hhhhhhhhhhhhhhhhhhcchhhhhhhhhhhhhhhhcccc

Prosite for DKFZphfkd2_47a4.1

PS00001 44->48 ASN_GLYCOSYLATION PDOC00001 PS00001 107->111 ASN_GLYCOSYLATION PDOC00001 PS00004 27->31 CAMP_PHOSPHO_SITE PDOC00004 PS00005 154->157 PKC_PHOSPHO_SITE PDOC00005 PS00005 160->163 PKC_PHOSPHO_SITE PDOC00005 PS00006 160->164 CK2_PHOSPHO_SITE PDOC00006 PS00006 194->198 CK2_PHOSPHO_SITE PDOC00006 PS00006 215->219 CK2_PHOSPHO_SITE PDOC00006 PS00007 178->185 TYR_PHOSPHO_SITE PDOC00007 PS00007 13->22 TYR_PHOSPHO_SITE PDOC00007 PS00008 124->130 MYRISTYL PDOC00008 PS00028 148-M71 ZINC FINGER C2H2 PDOC00028

Pfam for DKFZphfkd2_47a4.1

HMM_NAME Zmc finger, C2H2 type

HMM *CpwPDCgKtFrrwsNLrRHMR..T.H*

C + C+KTFR + +L+ HMR H Query 148 CLY--CEKTFRGKNTLKDHMRKK-QH 170

DKFZphfkd2_4b6

group: kidney derived

DKFZphfkd2_4b6 encodes a novel 133 amino acid protein with similarity to Homo sapiens clone 25003 partial CDS.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of kidney-specific genes . similarity to Homo sapiens clone 25003 complete cDNA, complete eds, few EST hits

Sequenced by GBF

Locus : unknown

Insert length: 1936 bp

Poly A stretch at pos. 1916, polyadenylation signal at pos. 1890

1 GGGAGACTTG CAATGAAGTT AGAATGAACA GGAGGAGTCT GCAGCTTTTC

51 AGTGCCTGGG ATAACTATAG TTTAAAGATC ATTGTGTAAA ATAGGATTTT

101 TAGTCAGCAT GCATTGTTTT AAACCGACTA ACTGATAGCC TAAAACTTTA

151 TTTTTGCATT TTGCCAATCC TTGGAGTTTT GTTTTGCAGA ATTAAGAAAA

201 AAATGAATGT ATGATCATCT GAAAAGGGCT TTCTCTCAAT CCCACTTCAT

251 GGCATGACCT CTGCTGGATC ATTAGTTCTA GCCAGAGAAG TAGCAAAGGA

301 ACATGACGTC TGAGACCTCC CTTCCCTCAT CAGTGGGGCT GACTGAGCTG

351 GGGGCTTGAA GCCGGAGGTA ACCTTTCCTG TCGAATGTTT CTTTAGAGAA

401 TGGCAATGGT CTCTGCGATG TCCTGGGTCC TGTATTTGTG GATAAGTGCT

451 TGTGCAATGC TACTCTGCCA TGGATCCCTT CAGCACACTT TCCAGCAGCA

501 TCACCTGCAC AGACCAGAAG GAGGGACGTG TGAAGTGATA GCAGCACACC

551 GATGTTGCAA CAAGAATCGC ATTGAGGAGC GGTCACAAAC AGTAAAGTGT

601 TCCTGTCTAC CTGGAAAAGT GGCTGGAACA ACAAGAAACC GGCCTTCTTG

651 CGTCGATGCC TCCATAGTGA TTTGGAAATG GTGGTGTGAG ATGGAGCCTT

701 GCCTAGAAGG AGAAGAATGT AAGACACTCC CTGACAATTC TGGATGGATG

751 TGCGCAACAG GCAACAAAAT TAAGACCACG AGAATTCACC CAAGAACCTA

801 ACAGAAGCAT TTGTGGTAGT AAAGGAAAAC CAACCCTCTG GAAAATACAT

851 TTTGAGAATC TCAAACATCT CACATATATA CAAGCCAAAT GGATTTCTTA

901 CTTGCACTTT GACTGGCTAC CAGATAATCA CAGTGCGTTT AGTGTGTGTA

951 ACGAAATATC CTACAGTGAG AAGACACAGC GTTTTGGCAT CACCATGGAA

1001 AGTGGGCTTA AAAAAGGGTC TTCTCAGTGA AATTTTTGGG CATCATGAAG

1051 AACGATCAAC TATCTTCTAA TTTGAATCTA TAGTTACTTT GTACCATTTG

1101 AAATATATGT ATATATATAT ATATAATATT TTGAAATATT ATCTATTCTC

1151 TTCAAGAAAT GAACAGTACC ACAGTTTGAG ACGGCTGGTG TACCCCTTTG

1201 AGTTTTGGAT GTTTTGTCTG TTTTGCTTTG TTTTGTTAGT CATTTCTTTT

1251 TCTAACGGCA AGGAAGATAT GTGCCCTTTT GAGAATTCAA GATGGCACTG

1301 ACACGGGAAG GCCAGCTACA GGTGGACTCC TGGAATTTGA GGCATCATAA

1351 TGATACTGAA TCAAGAACTT CCTTCTGCTT CTACCAGATG GCCCAAGGAA

1401 GCACATCGTC CTGTTTTATT GCTTTCTACC CTGTGCAATA TTAGCATGCA

1451 AGCTTGGCTT ACATAGTCAT ACTTTATATT CAATTGATAT ATAATAACCG

1501 TTCTAACCTC TTCCAGGAAA ATATTTTTAG AACTACTAGC TTTTCCACTT

1551 AGAAGAAAAT GAGGATTCTT AAGGGAGCCA CTCCACCATG CTATTAAGAC

1601 TCTGGCAGAG TTATGGGTAG GATATGGATC CCTACATGAA TAAGTCCTGT

1651 AAATACAATG TCTTAAGGCT TTGTATAGCT GTCCTAGACT GCAGAAATGT

1701 CCTCTGATTA AATCCAAAGT CTGGCATCGT TAACTACATA GTGCTGTAGC

1751 AACAAGTCTT ATCATGGCAT CTCTTTCTAT GTTTGGTTTG CTTTTTCCAA

1801 GAGTATTCAG GTCTCCTCTT GTGAGATAGG AAGGCCATGA AAACAATTAG

1851 ATTTCAAGAT GATCTATGTG ACCAAATGTT GGACAGCCCT ATTAAAGTGG

1901 TAAACAACTT CTTTCTAAAA AAAAAAAAAA AAAAAA

BLAST Results o BLAST result

Medl e entries o Medlme entry Peptide information for frame 1

ORF from 400 bp to 798 bp; peptide length: 133 Category: similarity to unknown protein Classification: no clue

1 MAMVSAMSWV LYLWISACAM LLCHGSLQHT FQQHHLHRPE GGTCEVIAAH 51 RCCNKNRIEE RSQTVKCSCL PGKVAGTTRN RPSCVDASIV IWKWWCEMEP 101 CLEGEECKTL PDNSGWMCAT GNKIKTTRIH PRT

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfkd2_4b6, frame 1

TREMBLNEW:AF131851_1 product: "Unknown"; Homo sapiens clone 25003 mRNA sequence, partial eds., N = 1, Score = 242, P = 1.7e-20

>TREMBLNEW:AF131851_1 product: "Unknown"; Homo sapiens clone 25003 mRNA sequence, partial eds. Length = 165

HSPs:

Score = 242 (36.3 bits), Expect = 1.7e-20, P = 1.7e-20 Identities = 44/89 (49%), Positives = 58/89 (65%)

Query: 42 GTCEVIAAHRCCNKNRIEERSQTVKCSCLPGKVAGTTRNRPSCVDASIVIWKWWCEMEPC 101

GTCE++ R ++ R QT +C+C G++AGTTR RP+CVDA 1+ K WC+M PC Sbjct: 76 GTCEIVTLDRDSΞQPRRTIARQTARCACRKGQIAGTTRARPACVDARIIKTKQWCDMLPC 135

Query: 102 LEGEECKTLPDNSGWMCAT-GNKIKTTRI 129

LEGE C L + SGW C G +IKTT + Sbjct: 136 LEGEGCDLLINRSGWTCTQPGGRIKTTTV 164

Pedant information for DKFZphf d2_4b6, frame 1

Report for DKFZphf d2_4b6.1

[LENGTH] 133

[MW] 15030.64

[pi] 8.49

[HOMOL] TREMBLNEW:AF131851_1 product: "Unknown"; Homo sapiens clone 25003 mRNA sequence, partial eds. 4e-20

[KW] Alpha_Beta

[KW] SIGNAL_PEPTIDE 26

SEQ MAMVSAMSWVLYLWISACAMLLCHGSLQHTFQQHHLHRPEGGTCEVIAAHRCCNKNRIEE PRD ccchhhhhhhhhhhhhhhhhhhhccccchhhhhhhcccccccceeeeeeecccccchhhh

SEQ RSQTVKCSCLPGKVAGTTRNRPΞCVDASIVIWKWWCEMEPCLEGEECKTLPDNSGWMCAT PRD hhhhhhccccccccccccccccccceeeeeehhhhhhccccccccceeeecccccceeec

SEQ GNKIKTTRIHPRT PRD ccccccccccccc

(No Prosite data available for DKFZphfkd2_4b6.1) (No Pfam data available for DKFZphfkd2_4b6.1) DKFZphfkd2_4c8 group: kidney derived

DKFZphfkd2_4c8 encodes a novel 153 amino acid protein with partial similarity to huntington's associated protein HAP1.

The novel protein contains a leucine zipper involved in protem-protem interaction. No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application m studying the expression profile of kidney-specific genes .

similarity to KIAA0549 and HAP1 potential frame shift at Bp -1350-1500 will be checked

Sequenced by GBF

Locus : unknown

Insert length: 3182 bp

Poly A stretch at pos. 3162, polyadenylation signal at pos. 3135

1 GGGCTTCCCC CATAGAATTT TTCTTTTCAT TGCCCACTTT ACTGTTTTGG

51 CTCCAGACTG TCGTTAAGAA TGTACAGCCT AATTCTGGTG TGTTTCGGGA

101 TATTCTTCTG TCCAGTATTC TGGAAGGGCG GGGAGGCATG GCAGCGTTTT

151 ACTTGACGTT GATGGTGCTG TGAAGTCCAT TCTTTCCTCT GCAAGACTAC

201 TGACTATGCA GAAATTTATC GAAGCGGATT ATTATGAACT AGACTGGTAT

251 TATGAAGAAT GCTCGGATGT TTTATGTGCT GAAAGAGTTG GCCAGATGAC

301 TAAGACATAT AATGACATAG ATGCTGTCAC TCGGCTTCTT GAGGAGAAAG

351 AGCGGGATTT AGAATTGGCC GCTCGCATCG GCCAGTCGTT GTTGAAGAAG

401 AACAAGACCC TAACCGAGAG GAACGAGCTG CTGGAGGAGC AGGTGGAACA

451 CATCAGGGAG GAGGTGTCTC AGCTCCGGCA TGAGCTGTCC ATGAAGGATG

501 AGCTGCTTCA GTTCTACACC AGCGCAGCGG AGGAGAGTGA GCCCGAGTCC

551 GTTTGCTCAA CCCCGTTGAA GAGGAATGAG TCGTCCTCCT CAGTCCAGAA

601 TTACTTTCAT TTGGATTCTC TTCAAAAGAA GCTGAAAGAC CTTGAAGAGG

651 AGAATGTTGT ACTTCGATCC GAGGCCAGCC AGCTGAAGAC AGAGACCATC

701 ACCTATGAGG AGAAGGAGCA GCAGCTGGTC AATGACTGCG TGAAGGAGCT

751 GAGGGATGCC AATGTCCAGA TTGCTAGTAT CTCAGAGGAA CTGGCCAAGA

801 AGACGGAAGA TGCTGCCCGC CAGCAAGAGG AGATCACACA CCTGCTATCG

851 CAAATAGTTG ATTTGCAGAA AAAGGCAAAA GCTTGCGCAG TGGAAAATGA

901 AGAACTTGTC CAGCATCTGG GGGCTGCTAA GGATGCCCAG CGGCAGCTCA

951 CAGCCGAGCT GCGTGAGCTG GAGGACAAGT ACGCAGAGTG CATGGAGATG

1001 CTGCATGAGG CGCAGGAGGA GCTGAAGAAC CTCCGGAACA AAACCATGCC

1051 CAATACCACG TCTCGGCGCT ACCACTCACT GGGCCTGTTT CCCATGGATT

1101 CCTTGGCAGC AGAGATTGAG GGAACGATGC GCAAGGAGCT GCAGTTGGAA

1151 GAGGCCGAGT CTCCAGACAT CACTCACCAG AAGCGTGTCT TTGAGACAGT

1201 AAGAAACATC AACCAGGTTG TCAAGCAGAG ATCTCTGACC CCTTCTCCCA

1251 TGAACATCCC CGGCTCCAAC CAGTCCTCGG CCATGAACTC CCTCCTGTCC

1301 AGCTGCGTCA GCACCCCCCG GTCCAGCTTC TACGGCAGCG ACATAGGCAA

1351 CGTCGTCCTC GACAACAAGA CCAACAGCAT CATTCTGGAA ACAGAGGCAG

1401 CCGACCTGGG AAACGATGAG CGGAGTAAGA AGCCGGGGAC GCCGGGCACC

1451 CCCAGGCTCC CACGACCTGG AGACGGCGCT GAGGCGGCTG TCCCTGCGCC

1501 GGGAGAACTA CCTCTCGGAG AGGAGGTTCT TTGAGGAGGA GCAAGAGAGG

1551 AAGCTCCAGG AGCTGGCGGA GAAGGGCGAG CTGCGCAGCG GCTCCCTCAC

1601 ACCCACTGAG AGCATCATGT CCCTGGGCAC GCACTCCCGC TTCTCCGAGT

1651 TCACCGGCTT CTCTGGCATG TCCTTCAGCA GCCGCTCCTA CCTGCCTGAG

1701 AAGCTCCAGA TCGTGAAGCC GCTGGAAGGT GATCACGCGG GGCCTCGGCC

1751 CCTCTCTGTC CTCCTGGGGG ACTCCCTTTG GTCCCTGATC CACCTGCGGA

1801 AGGCGGGGCA CCTCTGTCAC GCCTACTCCT TTTTCTTCCG CGACAGCCAC

1851 CCGCGCTGCT GGTTTGAGTT CCTCTGAGGG TGGTGCTCAG CCTAGGCCTC

1901 CGTCCCTCCC CTCTGGCTGG CAGGTGTGAC AATGCACACA TAGGCCATGA

1951 AACTCGCCGA GGAAAGACAA GCATGTGCAC TGTGGTCTTC TAGTTCTTTC

2001 CTTTGCCTTT AGAACCTTAG AAATAAAAAC TTTTGTGGCG GTAGAGGCAC

2051 TGCTAACTGA TTCAAAAATT AATTAGGTTT TGCCTGTGGG TGTGAGGAAT

2101 GCAGAAAATT AATGCTTTAG CTTTTCTGCA GTTTTGGTGT CGGGGAGAGG

2151 TTCCAAGCAA ACTCTATTAA ATGGGGATTT TTTTTTCCCC ATAACCACCT

2201 GAATGTGATT TGTGGGCTTA TGTGTTCTGA TTTGAACTTC ATATAGCAAG

2251 GTTGTGGCTT TTGGCAGATG CAGTATGTTC TGAGCGCGGC TCCTAGAGTC

2301 TACAATTTGG AGTCCAGGAA GGGGTGGCTG TGGAGACAAG TGAGTTTTGT

2351 ACCTCCGTAA GCCACCCTTT TTCAGGGTCA GTTCATGTGT TAGTATCAGG

2401 GGCATCTCAG ATGATTAAAC TCATGGGAAA AACTTCCTCC TTCCCTCTCT

2451 CCCTCTTGCC CTCCTGCCTC TTTTTTTTTT TTTTTTTTTT AATTTGGGCA

2501 CTTATAAAAT GTTTTCCCTC TACCTGCTGC TACTCTGCCA AGAGCCACCA

2551 AGTGCTTATA TTTTTCATTT TTTACTCCTT TAGTTTGGAA AGCCATATAC

2601 GTTTGAGAAG GTGTTTTAAA ACTCTGTGTT ACACTTACGA TGCAAAGCCA

2651 AATCAGAACT TCTGTAAGGC AGAACTTTCC CAACTTTAAA AAAATTATTG 2701 TCCCCTCTAG GAGCCTTCTT AGACGTTTTT TCCTAATCAC CCCCCAAAGA

2751 CATTTTAATA CCACATATAT ATTGTTTATG TACTATATGT ATATACATAA

2801 ACAATACATA AGCAATACAT CTGTGGTATT AAAATTAAAA AGAATCCAAT

2851 TATGTTTACC TCAAAAGAAC CTGTTTTTGC TTCTTGGGAG CAATATTGCC

2901 CCTGTGAGAC TGCATGCTAT AAGGTAAGGT TGTGCTTGTT AAAGACCCAA

2951 GACATGACTG GGTTCCACAG TCTCCAAAGG AAGAGGGTGG GCTAGTTTGT

3001 TTTTATTATT ATTTTAAAAT TGTATAATTG GGGTCTTTCT TAGAGTTCAG

3051 AAAAGGTATA GCTTACTCTT TTTTAATTGT TTATTTAGTT GTAAGCTTAG

3101 TGATTGTTTT CTGATCCACA TTGTGTGTGT TCTTCAATAA AATCTTTCAT

3151 TTCTGCAATT TTAAAAAAAA "AAAAAAAAAA AA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 206 bp to 1531 bp; peptide length: 442

Category: similarity to known protein Classification: unset

Prosite motifs: LEUCINE ZIPPER (139-161)

1 MQKFIEADYY ELDWYYEECS DVLCAERVGQ MTKTYNDIDA VTRLLEEKER

51 DLELAARIGQ SLLKKNKTLT ERNELLEEQV EHIREEVSQL RHELSMKDEL

101 LQFYTSAAEE SEPESVCSTP LKRNESSSSV QNYFHLDSLQ KKLKDLEEEN

151 VVLRSEASQL KTETITYEEK EQQLVNDCVK ELRDANVQIA SISEELAKKT

201 EDAARQQEEI THLLSQIVDL QKKAKACAVE NEELVQHLGA AKDAQRQLTA

251 ELRELEDKYA ECMEMLHEAQ EELKNLRNKT MPNTTSRRYH SLGLFPMDSL

301 AAEIEGTMRK ELQLEEAESP DITHQKRVFE TVRNINQVVK QRSLTPSPMN

351 IPGSNQSSAM NSLLSSCVST PRSSFYGSDI GNVVLDNKTN SIILETEAAD

401 LGNDERSKKP GTPGTPRLPR PGDGAEAAVP APGELPLGEE VL

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfkd2_4c8, frame 2

PIR:S72555 huntingtin-associated protein HAP1 - human (fragment), N = 1, Score = 234, P = 8.6e-19

TREMBL :CEUT27A3_7 gene: "T27A3.1"; Caenorhabditis elegans cosmid T27A3., N = 1, Score = 226, P = 9.9e-16

PIR:S67495 huntingtin-associated protein HAP1-A - rat, N = 1, Score = 215, P = 1.6e-14

>PIR:S72555 huntingtin-associated protein HAP1 - human (fragment) Length = 320

HSPs:

Score = 234 (35.1 bits), Expect = 8.6e-19, P = 8.6e-19 Identities = 66/189 (34%), Positives = 110/189 (58%)

Query: 109 EESEPESVCSTPLKRNE—SSSSVQNYFH LDSLQKKLKDLEEENVVLRSEASQLKTE 163

EE+E + C+ P + S ++ + H L++LQ+KL+ LEEEN LR EASQL T Sbjct: 28 EEAEEDLQCAHPCDAPKLISQEALLHQHHCPQLEALQEKLRLLEEENHQLREEASQLDT- 86

Query: 164 TITYEEKEQQLVNDCVKELRDANVQIASISEELAKKTEDAARQQEEITHLLSQIVDLQKK 223

E++EQ L+ +CV++ +A+ Q+A +SE L + E+ RQQ+E+ L +Q++ LQ++ Sbjct: 87 LEDEEQMLILECVEQFSEASQQMAELSEVLVLRLENYERQQQEVARLQAQVLKLQQR 143

Query: 224 AKACAVENEELVQHLGAAKDAQRQLTAE—LRELEDKYAECME--MLHEAQEELKNL-RN 278 + E E+L + L + K+ Q QL E L ++ AE + + + + + RN Sbjct: 144 CRMYGAETEKLQKQLASEKEIQMQLQEEETLPGFQETLAEELRTSLRRMISDPVYFMERN 203

Query: 279 KTMP--NTTSRRY 289

MP +T+S RY Sbjct: 204 YEMPRGDTSSLRY 216

Peptide information for frame 3

ORF from 1416 bp to 1874 bp; peptide length: 153 Category: similarity to known protein Classification: unset

1 MSGVRSRGRR APPGSHDLET ALRRLSLRRE NYLSERRFFE EEQERKLQEL

51 AEKGELRSGS LTPTESIMSL GTHSRFSEFT GFSGMSFSSR SYLPEKLQIV

101 KPLEGDHAGP RPLSVLLGDS LWSLIHLRKA GHLCHAYΞFF FRDSHPRCWF 151 EFL

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfkd2_4c8, frame 3

TREMBL:AB011121_1 gene: "KIAA0549"; product: "KIAA0549 protein"; Homo sapiens mRNA for KIAA0549 protein, partial eds., N = 1, Score = 252, P = 5.5e-21

>TREMBL:AB011121_1 gene: "KIAA0549"; product: "KIAA0549 protein"; Homo sapiens mRNA for KIAA0549 protein, partial eds. Length = 469

HSPs:

Score = 252 (37.8 bits), Expect = 5.5e-21, P = 5.5e-21 Identities = 57/98 (58%), Positives = 69/98 (70%)

Query 8 GRRAPPGSHDLETALRRLSLRRENYLSERRFFEEEQERKLQELAEKGELRSGSLTPTESI 67

G+ P G DL TAL RLSLRR+NYLSE++FF EE +RK+Q LA++ E SG +TPTES+ Sbjct 27 GQPGPSGDSDLATALHRLSLRRQNYLSEKQFFAEEWQRKIQVLADQKEGVSGCVTPTESL 86 Query 68 MSLGTHSRFSEFTGFSGMSFSSRSYLPEKLQIVKPLEG 105

SL T SE T S S R ++PEKLQIVKPLEG Sbjct 87 ASLCTTQ—SEITDLSSAΞ-CLRGFMPEKLQIVKPLEG 121

Pedant information for DKFZphfkd2_4c8, frame 2

Report for DKFZphfkd2_4c8.2

[LENGTH] 442 [MW] 50020.14 [pi] 4.77 [HOMOL] TREMBL :AF040723_1 product: "neuroanl' Homo sapiens neuroanl mRNA, complete eds. 5e-29 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL058w] 5e-08 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YIL149c] 5e-08 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDL058w] 5e-08 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YILl38c] 6e-08 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YGR130c] 2e-07 [ FUNCATj 09.10 nuclear biogenesis [S. cerevisiae, YDR356w] le-06 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YDR356w] le-06 [FUNCAT] 1 genome replication, transcription, recombination and repair [M. annaschii MJ1643] le-06 [FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YHR023w MYOl - myosιn-1 soform] 3e-06 [FUNCAT] 03.25 cytokinesis [S. cerevisiae, YHR023w MYOl - myosιn-1 isoform] 3e-06 [FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YKR095w] 4e-06 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKR095w] 4e-06 [FUNCAT] 03.13 meiosis [S. cerevisiae, YNL250w] 2e-05 [FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YNL250w] 2e-05 [FUNCAT] 08.99 other intracellular-transport activities [S. cerevisiae, YNL079c]

5e-05 FUNCAT] 03.01 cell growth [S. cerevisiae, YNL079c] 5e-05 FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [S. cerevisiae, YNL079c] 5e-05

FUNCAT] 10.05.99 other pheromone response activities [S. cerevisiae, YHR158c] e-04 FUNCAT] 30.13 organization of chromosome structure [S. cerevisiae, YDR285w] le-04 FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YNL272C] 3e-04 FUNCAT] 08.16 extracellular transport [S. cerevisiae, YNL272c] 3e-04 BLOCKS] BL01289B BLOCKS] BL00415M Synapsms proteins EC] 3.6.1.32 Myosin ATPase 2e-07

PIRKW] tandem repeat 2e-07 PIRKW] heterodimer le-06 PIRKW] endocytosis 9e-07 PIRKW] heart le-06 PIRKW] transmembrane protein 4e-07 PIRKW] zinc finger 9e-07 PIRKW] metal binding 9e-07 PIRKW] DNA binding 3e-06 PIRKW] muscle contraction 2e-07 PIRKW] acetylated ammo end 3e-06 PIRKW] actin binding 2e-07 PIRKW] mitosis le-06 PIRKW] microtubule binding le-06 PIRKW] ATP 2e-07 PIRKW] chromosomal protein le-06 PIRKW] receptor 3e-08 PIRKW] thick filament 2e-07 PIRKW] phosphoprotem 8e-06 PIRKW] glycoprotein 3e-08 PIRKW] skeletal muscle 3e-06 PIRKW] DNA condensation le-06 PIRKW] alternative splicing 2e-06 PIRKW] coiled coil 2e-07 PIRKW] P-loop 2e-07 PIRKW] heptad repeat 4e-07 PIRKW] methylated amino acid 2e-07 PIRKW] peripheral membrane protein 9e-07 PIRKW] cardiac muscle 6e-06 PIRKW] hydrolase 2e-07 PIRKW] muscle 2e-06 PIRKW] cytoskeleton 2e-06 PIRKW] Golgi apparatus 4e-07 PIRKW] calmodulin binding 9e-07 SUPFAM] myosin motor domain homology 2e-07 SUPFAM] tropomyosm TPM1 2e-06 SUPFAM] giantm 4e-07 SUPFAM] protein kinase C zinc-binding repeat homology 2e-06 SUPFAM] human early endosome antigen 1 9e-07 SUPFAM] unassigned kmesm-related proteins 4e-07 SUPFAM] M5 protein 8e-08 SUPFAM] cytoskeletal keratin 3e-06 SUPFAM] myosin heavy chain 2e-07 SUPFAM] conserved hypothetical P115 protein le-06 SUPFAM] centromere protein E le-06 SUPFAM] pleckstπn repeat homology 2e-06 SUPFAM] kinesin motor domain homology 4e-07 PROSITE] LEUCINE_ZIPPER 1 KW] All_Alpha KW] LOW_COMPLEXITY 6.79 % KW] COILED COIL 27.15 %

SEQ MQKFIEADYYELDWYYEECSDVLCAERVGQMTKTYNDIDAVTRLLEEKERDLELAARIGQ

SEG xxxxxxxxxxxxxxx ...

PRD ccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS C

SEQ SLLKKNKTLTERNELLEEQVEHIREEVSQLRHELSMKDELLQFYTSAAEESEPESVCSTP SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ LKRNESSSSVQNYFHLDSLQKKLKDLEEENVVLRSEASQLKTETITYEEKEQQLVNDCVK SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ ELRDANVQIASISEELAKKTEDAARQQEEITHLLSQIVDLQKKAKACAVENEELVQHLGA

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCC

SEQ AKDAQRQLTAELRELEDKYAECMEMLHEAQEELKNLRNKTMPNTTSRRYHSLGLFPMDSL

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ AAEIEGTMRKELQLEEAESPDITHQKRVFETVRNINQVVKQRSLTPSPMNIPGSNQSSAM

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccchhhh

COILS

SEQ NSLLSSCVSTPRSSFYGSDIGNVVLDNKTNSIILETEAADLGNDERSKKPGTPGTPRLPR

SEG xxxxxxxxxxx

PRD hhhhhcccccccccccccccceeeeeccccceeecccccccccccccccccccccccccc

COILS

SEQ PGDGAEAAVPAPGELPLGEEVL

SEG xxxx

PRD cccccccccccccccccccccc

COILS

Prosite for DKFZphfkd2_4c8.2 PS00029 139->161 LEUCINE_ZIPPER PDOC00029

(No Pfam data available for DKFZphfkd2_4c8.2)

Pedant information for DKFZphfkd2_4c8, frame 3

Report for DKFZphfkd2_4c8.3

[LENGTH] 153

[MW] 17642.03

[pi] 9.38

[HOMOL] TREMBL:AB011121_1 gene: "KIAA0549"; product: "KIAA0549 protein"; Homo sapiens mRNA for KIAA0549 protein, partial eds. 2e-12

[KW] Alpha_Beta

[KW] LOW_COMPLEXITY 12.42 %

SEQ MSGVRSRGRRAPPGSHDLETALRRLSLRRENYLSERRFFEEEQERKLQELAEKGELRSGS

SEG xxxxxxxxxxxxxxxxxxx

PRD cccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccc

SEQ LTPTESIMSLGTHSRFSEFTGFSGMSFSSRSYLPEKLQIVKPLEGDHAGPRPLSVLLGDS SEG

PRD cccccceeeccccceeeccccccccccccccccchhhhhhhhcccccccccceeeeeccc

SEQ LWSLIHLRKAGHLCHAYSFFFRDSHPRCWFEFL SEG

PRD chhhhhhhhhcccccceeeeecccccccccccc

(No Prosite data available for DKFZphfkd2_4c8.3) (No Pfam data available for DKFZphfkd2_4c8.3) DKFZphfkd2_4kl4

group: intracellular transport and trafficking

DKFZphfkd2_4kl4.3 encodes a novel 254 amino acid putative GTP-bmdmg protein nearly identical to Rab6.

Rab proteins are members of the Ras superfamily of GTPases. Rab proteins are localised to the cytoplasmic side of organelles and vesicles involved in the secretory (biosynthetic) and endocytotic pathways in eukaryotic cells. Rab proteins direct the targeting and fusion of transport vesicles to their acceptor membranes. rab6 is a ubiquitous ras-like GTPase involved in mtra-Golgi transport.

The new protein can find application in modulating the transport of vesicles inside the Golgi apparatus . strong similarity to Rab6 complete cDNA, complete eds, EST hits

Sequenced by GBF

Locus: unknown

Insert length: 3084 bp

Poly A stretch at pos. 3061, polyadenylation signal at pos. 3043

1 GGGGCACTCA GCAGGTTGGG CTGCGGCGGC GGCGGCTGGG GAAGCCGAAG 51 CGCCGCGCGT GAGAGATCCC GGATACATCT GCGGTTTGGG CTCCGCCACC

101 CTCCGTCTCT CTCCCGCAGG TCTCTGAGCC GGGTGCGGAA GGAGGGAACG

151 GCCCTAGCCT TGGGAAGCCA AAGCACACCC CTGGCTCCCG CCGACACCGC

201 CCTCCTTCCC TTCCCAGCCG CGGGCCTCGC TCCGTGCTCG GCTACTCTGC

251 CGGGAGGCGG CGGCGGCTGC CAGTCTGTGG CGAGCCCTGC TGCCCTCCAG

301 CCGGGCTTCT CCAGCCGGGC TCCTCCACCG GCCCTTGCAG GGGCACAGAG

351 AGCTCGGCGC CCGCCCTTCC GCTCGCCTTT TTCGTCAGCC GGCTGGAGGA

401 GCATCGGTCC GGGAGGTCTC TGGGCTGAGG CGGCGACAGC TCCTCTAGTT

451 CCACCATGTC CGCGGGCGGA GACTTCGGGA ATCCGCTGAG GAAATTCAAG

501 CTGGTGTTCC TGGGGGAGCA AAGCGTTGCA AAGACATCTT TGATCACCAG

551 ATTCAGGTAT GACAGTTTTG ACAACACCTA TCAGGCAATA ATTGGCATTG

601 ACTTTTTATC AAAAACTATG TACTTGGAGG ATGGAACAAT CGGGCTTCGG

651 CTGTGGGATA CGGCGGGTCA GGAACGTCTC CGTAGCCTCA TTCCCAGGTA

701 CATCCGTGAT TCTGCTGCAG CTGTAGTAGT TTACGATATC ACAAATGTTA

751 ACTCATTCCA GCAAACTACA AAGTGGATTG ATGATGTCAG AACAGAAAGA

801 GGAAGTGATG TTATCATCAC GCTAGTAGGA AATAGAACAG ATCTTGCTGA

851 CAAGAGGCAA GTGTCAGTTG AGGAGGGAGA GAGGAAAGCC AAAGGGCTGA

901 ATGTTACGTT TATTGAAACT AGGGCAAAAA CTGGATACAA TGTAAAGCAG

951 CTCTTTCGAC GTGTAGCAGC AGCTTTGCCG GGAATGGAAA GCACACAGGA 1001 CGGAAGCAGA GAAGACATGA GTGACATAAA ACTGGAAAAG CCTCAGGAGC 1051 AAACAGTCAG CGAAGGGGGT TGTTCCTGCT ACTCTCCCAT GTCATCTTCA 1101 ACCCTTCCTC AGAAGCCCCC TTACTCTTTC ATTGACTGCA GTGTGAATAT 1151 TGGCTTGAAC CTTTTCCCTT CATTAATAAC GTTTTGCAAT TCATCATTGC 1201 TGCCTGTCTC GTGGAGGTGA TCTATTAGCT TCACAAGCAC AAAAAAAGTC 1251 AGCGTCTTCA TTATTTATAT TTTACAAAAA GCCAAATTAT TTCAGCATAT 1301 TCCGGTGATA ACTTTAAAAA TTAGATACAT TTTCTTAACA TTTTTTTCTT 1351 TTTTAATGTT ATGATAATGT ACTTCAAAAT GATGGAAATC TCAACAGTAT 1401 GAGTATGGCT TGGTTAACGA GCAGTATGTT CACAGCCTGC TTTATCTCTC 1451 CTTGCTCTTC TCACCTCTCC CTTACCCCGT TCCCTATTTC CGTGTTCTTA 1501 CCTAGCCTCC CCCCACTTCC TCAAAACAAA CAAGAGATGG CAAAGCAGCA 1551 GTCCGACCAA GCCCACTGGA ATTATCCTTT AATTTTACAG ATACCACTTG 1601 CTGTAGGCTG TGGACCAAGA TGTCCAGAAT TATTCTTGAG CACTGATGTA 1651 AATTACTTAG ATCTTCTTTG AGGTCAGAAT TCAGCGATCA CGGTAGGCAG 1701 TGCTTGAATG AGAAAAGCCT CCTGGTGCAT CTTCAAAATG AGTCCTAAAG 1751 AACATACTGA GTACTTATAA GTAGCAGAAC ATAAAATGTA TTTCTGACTA 1801 ACACAAATGG TCCTTTCACA TGTGCTTTAT TAGACTCTGG GAGAGAAAAG 1851 TAACCAAGTG CTTCAGAACA GGTTTTTAGT ATTTACTTCT TCATGGTAAG 1901 ATAATGAAGT TCTAATGAAC TATTTCTCCC AAGGTTTTAA AATTGTCAAG 1951 AGTTATTCTG TTTGTTTAAA AAGTAAGAAA CCTCTGTAAG CAATAGATTT 2001 TGCTTGGGTT TTCTTTCTTA AAAAAATAAT ACTATGCAGG CAAGACACCA 2051 TAAAAGTTTA ATTCCTTACA GAAGAACCAG TGGAAGAATT TAAATTTGGC 2101 ACTACGATCA AAACTACTGA ATTAGCAGAA ATAACGATAT CTAAAGCTTA 2151 CCAGCAAAAG AACCCTCAGC AGAATAGCAA AAACTTTGCT CAGGACATTT 2201 GAGGTCAAAT TGAAGACGGA AGACGGAAAC CGGAAACCGT TTTCTTGTAA 2251 GCCCCTAGAG GCAGATCAGG TAAGCATACA TAGTAGAGGG AAAGGAGAGA 2301 ATGGAAATAA AACTGAATAT TATGCAGATT TATGCCTTAT TTTTTAGCAT 2351 TTTTTAAGGT TGGGTCTTTC AGGCTGGTTT TGGTTTGTAT TAGATCTGTA 2401 TAGTTTAGTG ATTTAGTTTT ATATTTAAGC TACGATTAAT ATTTTTTCTT 2451 TGGCGATATT TCTTTGCTTT TTTTTTTTAA CAACTTTCCA TTTTTAGATG 2501 TTTCGTTGAA TCTATTTAGA GCTTCACCAT GGCAATATGT ATTTCCCTTA

2551 AAACACTGCA AACAAATATA CTAGGAGTGT GCCCTTTTAA TCTTTACTAG

2601 TTATTGTGAG ACTGCTGTGT AAGCTAATAA ACACATTTGT AAAAACATTG

2651 TTTGCAGGAA GAAAACTTCG AGTTACAGGT CAGGAAAAGC CTGCTGAATT

2701 TATGTTGTAA ACGTTACTTA ACACAGTATA AAGATGAAAA GACAACAAAA

2751 GTATCTTCAT ACTTCCTCAT CCCCTCATTG CAACAAAACC TTAAACTGGG

2801 AGAACCTTAG TCCCCTCTCT TTCCTCTTCC TCCTCCACTT CCCACTTATT

2851 GCCACTTTGT AATATTCAGA GAGCACTTGG ATTATGGATC TGAATAGAGA

2901 AATGCTTACA GATAATCATT AGCCCACATA CCAGTAACTT ATACTTAAAG

2951 ATGGGATGGA GTTATAAAGT GCTTTTATAA TCCAATATAA TTGCTAAAGG

3001 CAAGGGTTGA CTCTTTGTTT TATTTTGACA TGGCATGTCC TGAAATAAAT

3051 ATTGGTTCAC TATGAAAAAA AAAAAAAAAA AAAA

BLAST Results

No BLAST result

Medline entries

98382468: Rab proteins.

97203146:

GTP-bound forms of rab6 induce the redistribution of Golgi proteins into the endoplasmic reticulum.

Peptide information for frame 3

ORF from 456 bp to 1217 bp; peptide length: 254 Category: strong similarity to known protein Classif cation: unset Prosite motifs: BACTERIAL OPΞIN RET (45-57)

1 MΞAGGDFGNP LRKFKLVFLG EQSVAKTSLI TRFRYDSFDN TYQAIIGIDF 51 LSKTMYLEDG TIGLRLWDTA GQERLRSLIP RYIRDSAAAV VVYDITNVNS 101 FQQTTKWIDD VRTERGSDVI ITLVGNRTDL ADKRQVSVEE GERKAKGLNV 151 TFIETRAKTG YNVKQLFRRV AAALPGMEST QDGSREDMSD IKLEKPQEQT 201 VSEGGCΞCYS PMSSSTLPQK PPYSFIDCSV NIGLNLFPSL ITFCNSSLLP 251 VSWR

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphf d2_4kl4, frame 3

PIR:G34323 GTP-binding protein Rab6 - human, N = 1, Score = 944, P = 6.5e-95

TREMBL:CET25G12_2 gene: "T25G12.4"; Caenorhabditis elegans cosmid T25G12., N = 1, Score = 756, P = 5.4e-75

TREMBL : NTNTRAF_1 gene: "Nt-rab6"; Nicotiana tabacum SRI Nt-rab6 mRNA, complete eds., N = 1, Score = 698, P = 7.6e-69

TREMBL :D8431 _1 product: "rab6"; Drosophila melanogaster mRNA for rab6, complete eds., N = 1, Score = 836, P = 1.9e-83

PIR:T01588 small GTP-binding protein F16B22.10 - Arabidopsis thaliana, N = 1, Score = 704, P = 1.8e-69

>PIR:G34323 GTP-bmdmg protein Rab6 - human Length = 208

HSPs:

Score = 944 (141.6 bits), Expect = 6.5e-95, P = 6.5e-95 Identities = 186/208 (89%), Positives = 190/208 (91%) Query: 1 MSAGGDFGNPLRKFKLVFLGEQSVAKTSLITRFRYDΞFDNTYQAIIGIDFLSKTMYLEDG 60

MS GGDFGNPLRKFKLVFLGEQSV KTSLITRF YDSFDNTYQA IGIDFLSKTMYLED Sbjct: 1 MSTGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDR 60

Query: 61 TIGLRLWDTAGQERLRSLIPRYIRDSAAAVVVYDITNVNSFQQTTKWIDDVRTERGSDVI 120

T+ L+LWDTAGQER RSLIP YIRDS AVVVYDITNVNSFQQTTKWIDDVRTERGSDVI Sbjct: 61 TVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNVNSFQQTTKWIDDVRTERGSDVI 120

Query: 121 ITLVGNRTDLADKRQVSVEEGERKAKGLNVTFIETRAKTGYNVKQLFRRVAAALPGMEST 180

I LVGN+TDLADKRQVS+EEGERKAK LNV FIET AK GYNVKQLFRRVAAALPGMEST Sbjct: 121 IMLVGNKTDLADKRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMEST 180

Query: 181 QDGΞREDMSDIKLEKPQEQTVSEGGCSC 208

QD SREDM DIKLEKPQEQ VSEGGCSC Sbjct: 181 QDRSREDMIDIKLEKPQEQPVSEGGCSC 208

Pedant information for DKFZphfkd2_4kl4, frame 3

Report for DKFZphfkd2_4kl4.3

[LENGTH] 254

[MW] 28385.29

[pi] 7.58

[HOMOL] PIR:G34323 GTP-bmding protein Rab6 - human le-102

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YLR262c]

7e-60

[FUNCAT] 30.08 organization of golgi [S. cerevisiae, YLR262c] 7e-60

[FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae,

YOR089C] 2e ^■33

[FUNCAT] 08.19 cellular import [S. cerevisiae, YOR089c] 2e-33

[FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YOR089c] 2e-33

[FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YOR089c]

2e-33

[FUNCAT] 09.09 biogenesis of intracellular transport vesicles [S. cerevisiae,

YGL210W] 3e •28

[FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YFL005w] 8e-27

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YFL005w]

8e-27

[FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YORlOlw]

2e-21

[FUNCAT] 11.10 cell death [S. cerevisiae, YORlOlw] 2e-21

[FUNCAT] 01.03.13 regulation of nucleotide metabolism [S. cerevisiae, YORlOlw]

2e-21

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YORlOlw] 2e-21

[FUNCAT] 03.99 other cell growth, cell division and dna synthesis activities [S. cerevisiae, YORlOlw] 2e-21

[FUNCAT] 10.04.07 g-proteins [S. cerevisiae, YORlOlw] 2e-21

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YNL098C] 6e-19

[FUNCAT] 11.01 stress response [S. cerevisiae, YNL098c] 6e-19

[FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YNL098C] 6e-19

[FUNCAT] 04.07 rna transport [S. cerevisiae, YOR185c] 6e-16

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YOR185c] 6e-16

[FUNCAT] 08.01 nuclear transport [S. cerevisiae, YOR185C] 6e-16

[FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YPR165w] 4e-13

[FUNCAT] 10.02.07 g-protems [S. cerevisiae, YPR165w] 4e-13

[FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YCR027c] 2e-09

[FUNCAT] 10.05.07 g-protems [S. cerevisiae, YLR229C] 8e-08

[FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins

[Ξ. cerevisiae, YLR229C] 8e-08 [FUNCAT] 03.01 cell growth [S. cerevisiae, YNL180c] le-05 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YOR094w] 5e-05 [BLOCKS] BL01115A GTP-bmding nuclear protein ran proteins [SCOP] dlas3_2 3.29.1.4.12 Transduem (alpha subunit), insertion domai le-32 [SCOP] dlmhl 3.29.1.4.2 Racl [Human (Homo sapiens) 2e-51 [SCOP] d5p21 3.29.1. 1 cH-p21 Ras protein [human (Homo sapiens) 7e-53 [SCOP] dlhura_ 3.29.1. 8 ADP-ribosylation factor 1 (ARFl) [human (Hom le-46 [SCOP] dla2kc_ 3.29.1. 5 Ran Nuclear transport factor-2 (NTF2) [Do 6e-60 [PIRKW] nucleus 2e-14 [PIRKW] cell cycle control 5e-15 [PIRKW] membrane trafficking 3e-71 [PIRKW] endoplasmic reticulum le-29 [PIRKW] phosphoprotein le-29 [PIRKW] prenylated cysteine 2e-36 [PIRKW]. signal transduction 5e-15 [PIRKW] transforming protein 5e-30 [PIRKW] puπne nucleotide binding le-26 [PIRKW] alternative splicing le-18 [PIRKW] P-loop 3e-71 [PIRKW] lipoprotein 2e-36

[PIRKW] proto-oncogene le-20

[PIRKW] methylated carboxyl end le-20

[PIRKW] membrane protein le-29

[PIRKW] GTP binding 3e-71

[PIRKW] thiolester bond le-29

[PIRKW] Golgi apparatus le-29

[SUPFAM] ras transforming protein le-76

[PROSITE] BACTERIAL_OPSIN_RET 1

[PFAM] Ras family (contains ATP/GTP binding P-loop)

[KW] Alpha_Beta

[KW] 3D

SEQ MSAGGDFGNPLRKFKLVFLGEQSVAKTSLITRFRYDSFDNTYQAIIGIDFLSKTMYLEDG lkao- CCEEEEEEECTTTTCHHHHHHHHHHCCCCCCCTTTTC-EEEEEEEEETTE

SEQ TIGLRLWDTAGQERLRSLIPRYIRDSAAAVVVYDITNVNSFQQTTKWIDDVRTERGSDVI lkao- EEEEEEEECCTTTTCHHHHHHHHHHCCEEEEEEETTTHHHHHHHHHHHHHHHHHTTTCCC

SEQ ITLVGNRTDLADKRQVSVEEGERKAKGLNVTFIETRAKTGYNVKQLFRRVAAALPGMEST lkao- EEEEEETTTTGGGCCCCHHHHHHHHHHHCCCEEECTTTTHHHHHHHHHHH

SEQ QDGSREDMSDIKLEKPQEQTVSEGGCΞCYSPMSSSTLPQKPPYSFIDCSVNIGLNLFPSL lkao-

SEQ ITFCNSSLLPVSWR lkao-

Prosite for DKFZphfkd2_4kl4.3

PS00327 45->57 BACTERIAL OPSIN RET PDOC00291

Pfam for DKFZphfkd2_4kl4.3

HMM_NAME Ras family (contains ATP/GTP binding P-loop)

HMM *KLVLIGDSGVGKSCLLIRFTQNeFnEeYIPTIGvDFYtKTIEIDGKtIK KLV++G+ +V K++L RF +++F++ Y + IG+DF++KT+++++ TI

Query 15 KLVFLGEQSVAKTSLITRFRYDSFDNTYQAIIGIDFLSKTMYLEDGTIG 63

HMM LQIWDTAGQERYRsMRPMYYRGAMGFMLVYDITNRqSFENIrNWweEIrR L +WDTAGQER RS+ P Y+R++ ++++VYDITN SF+ ++W++++R+

Query 64 LRLWDTAGQERLRSLIPRYIRDSAAAVVVYDITNVNSFQQTTKWIDDVRT 113

HMM HCDrDENVPIMLVGNKCDLEDQRQVStEEGQeFAREWGAIPFMETSAKTN + ++V+I LVGN +DL+D+RQVS EEG+ A+ ++ + F+ET AKT+

Query 114 ERG—SDVIITLVGNRTDLADKRQVSVEEGERKAKGLN-VTFIETRAKTG 160

HMM iNVEEAFMEIvRellqrMqe.q.NqteNinidQpsrnrk....rCCCIM* +NV++ F +++ +++ +++ + +++++++I+ ++++ + +C+ +

Query 161 YNVKQLFRRVAAALPGMESTQDGSREDMSDIKLEKPQEQTVSEGGCS-C 208

DKFZphfkd2_4mll

group: transmembrane protein

DKFZphfbr2-4mll encodes a novel 159 ammo acid protein with weak similarity to the putative membrane protein YMR034c of S. cerevisiae.

The novel protein contains 4 transmembrane regions .

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of kidney-specific genes and as a new marker of neuronal cells. weak similarity to YMR034c complete cDNA, complete eds, no EST hits

Sequenced by GBF

Locus : unknown

Insert length: 1749 bp

Poly A stretch at pos. 1727, polyadenylation signal at pos. 1713

1 GGGGTCCTCA AAGCCGCCGG AGCAACCCCC AGGTCTTTAC TTTACAATCG

51 GCAATTTGAC TTGCTCTGCT GCATGTCTGG AGGGACCAAG GAAAGTGTGG

101 AGACGCTCCA AGGATTAGGT GATCGGAGCT TGAAAAGAAA AAAAGCCAAA

151 CAAATAAACA AAACCCACCC ACCCTAACGA ATATGAGGCT GCTGGAGAGA

201 ATGAGGAAAG ACTGGTTCAT GGTCGGAATA GTGCTGGCGA TCGCTGGAGC

251 TAAACTGGAG CCGTCCATAG GGGTGAATGG GGGACCACTG AAGCCAGAAA

301 TAACTGTATC CTACATTGCT GTTGCAACAA TATTCTTTAA CAGTGGACTA

351 TCATTGAAAA CAGAGGAGCT GACCAGTGCT TTGGTGCATC TAAAACTGCA

401 TCTTTTTATT CAGATCTTTA CTCTTGCATT CTTCCCAGCA ACAATATGGC

451 TTTTTCTTCA GCTTTTATCA ATCACACCCA TCAACGAATG GCTTTTAAAA

501 GGTTTGCAGA CAGTAGGTTG CATGCCTCCG CCTGTGTCTT CTGCAGTGAT

551 TTTAACCAAG GCAGTTGGTG GAAATGAGGC AGCTGCAATA TTTAATTCAG

601 CCTTTGGAAG TTTTTTGGTA AGTAAACATA GTTTAACTTG TCTATTACAA

651 CTTTTGCTGT GATATTGTGT ATATGAAAGA TTTAGTGAAA GCTGGATTTG

701 TTTTACTCTT TGGTTAAGTA TAAAAATTGT TGAATCTTTT CATGTGCCAG

751 TATCCATACC CTGAAGAAAA GTAGTTAATG AATAAAGCAA ATGTTCTCTT

801 ACAATATATT TTGGAGGTTT GGATTTTAAA ATTCCATTTA ATGAATTCAA

851 GGAATCAATT AAAACACTAT GTGTCTCCTT ATAGAGGTTA TGTCAATATA

901 TTGATCATTT AATGAGGTCT TTTAGATTAT TATTATTTTG TATCATGGGA

951 CTGAGGATTT TGAAAAGGAA ACATGACCCA GCTGGTCAGA AAGGGAATGC

1001 TAATTTACTT GTTGACATGC CATTTATTTT GTACATTTCA CTGTCAAAGA

1051 AGCTACTGGC TTGGATGCTT CTGAGAAATC TATGTGAGAA AAAATTTGAA

1101 AGGAAGATAT GACTAATGAG TAATTTGCAA GTAAATGTTG TATCTATATA

1151 TATATATATA TAAAGATTCA AAAGTAGTTC AGCTTTCATA AGTAGAACCA

1201 ATATAAGGAC GTTGTTTTAG CATTTTTAAT CATTATTTTT AAATAAATGA

1251 TGTAACAGAG GCTTGATTTG TGTTATGAAA GATTGAGAAA CTAAATTTTC

1301 TGTTGATTTA ATTTTTTTGT GCCTTAAAAC TTTGTTAAAT TCCTGAAGTT

1351 AATTATCATA TTGTACTTTT TGGGGCATAA CTCATTAGCA GATATGTAGT

1401 GCAGTGATTT ACAAATAATT GAGAGTAAAA TCAGTGATGT ATAAACTAGT

1451 TCATGAGTCT AGGTAAAATA TCAATTACCT CTGTTTAAAA TGCTCTGTTA

1501 ATTATTATTG TATGTATTTA AATGTAGTTA AAGCTTTTAA ACATGTTGTT

1551 ACATAGTGTT AATTCTACAC AGTGCTACAC AGCTTTTAGT GTCACATAGC

1601 CTTACAGAGT TTATAATGAT GTAGCATCTG CAAAATATAT GCATAGCTTA

1651 TATCCTATTT TTATAGAGCC AGTAATGGTT TTTGTGATGC TGTATTACTT

1701 CTGGGTTTTA GACAATAAAG TCTGTTTAAC AAAAAAAAAA AAAAAAAAA

BLAST Results o BLAST result

Medline entries o Medline entry

Peptide information for frame 3 ORF from 183 bp to 659 bp; peptide length: 159 Category: similarity to unknown protein

1 MRLLERMRKD WFMVGIVLAI AGAKLEPSIG VNGGPLKPEI TVSYIAVATI

51 FFNSGLSLKT EELTSALVHL KLHLFIQIFT LAFFPATIWL FLQLLSITPI

101 NEWLLKGLQT VGCMPPPVSS AVILTKAVGG NEAAAIFNSA FGSFLVSKHS

151 LTCLLQLLL

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphfkd2_4mll, frame 3

PIR:S53951 probable membrane protein YMR034c - yeast (Saccharomyces cerevisiae), N = 1, Score = 171, P = 3.2e-12

PIR:A65015 yfeH protein - Escherichia coli (strain K-12), N = 1, Score = 131, P = 4.2e-08

>PIR:S53951 probable membrane protein YMR034c - yeast (Saccharomyces cerevisiae)

Length = 434

HSPs:

Score = 171 (25.7 bits), Expect = 3.2e-12, P = 3.2e-12 Identities = 38/144 (26%), Positives = 72/144 (50%)

Query: 5 ERMRKDWFMVGIVLAIAGAKLEPSIGVNGGPLKPEITVSYIAVATIFFNSGLSLKTEELT 64

E ++ WF + + + I A+ P+ +GG +K + ++ Y VA IF SGL +K+ L Sbjct: 18 EFLKSQWFFICLAILIVIARFAPNFARDGGLIKGQYSIGYGCVAWIFLQSGLGMKSRSLM 77

Query: 65 SALVHLKLHLFIQIFTLAFFPATIWLF LQLLSITPINEWLLKGLQTVGCMPPPVSSA 121

+ +++ + H I + + + ++ F ++ + I++W+L GL P V+S Sbjct: 78 ANMLNWRAHATILVLSFLITSSIVYGFCCAVKAANDPKIDDWVLIGLILTATCPTTVASN 137

Query: 122 VILTKAVGGNEAAAIFNSAFGSFL 145

VI+T GGN + G+ L Sbjct: 138 VIMTTNAGGNSLLCVCEVFIGNLL 161

Pedant information for DKFZphfkd2_4mll, frame 3

Report for DKFZphfkd2_4mll .3

[LENGTH] 159

[MW] 17282.92

[pi] 9.06

[HOMOL] PIR:S53951 probable membrane protein YMR034C - yeast (Saccharomyces cerevisiae)

5e-12

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YMR034c] 2e-13

[PROSITE] MYRISTYL 2

[PROSITE] PKC_PHOSPHO_SITE 1

[KW] TRANSMEMBRANE 4

SEQ MRLLERMRKDWFMVGIVLAIAGAKLEPSIGVNGGPLKPEITVSYIAVATI FFNSGLSLKT PRD ccchhhhhhhhhhhhhhhhhhhhhcccccccccccccceeeeeeeccccccccccchhhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMM ..

SEQ EELTSALVHLKLHLFIQIFTLAFFPATIWLFLQLLSITPINEWLLKGLQTVGCMPPPVSΞ PRD hhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhccccchhhhhhhheeeecccccccc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ AVILTKAVGGNEAAAIFNSAFGSFLVSKHSLTCLLQLLL PRD ceeeeeccccchhhhhhhcccccceeecceeeeeeeccc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMM

Prosite for DKFZphf d2_4mll .3

PS00005 57->60 PKC_PHOSPHO_SITE PDOC00005 PS00008 15->21 MYRISTYL PDOC00008

PS00008 129->135 MYRISTYL PDOC00008

(No Pfam data available for DKFZphfkd2_4mll .3) PAGE INTENTIONALLY LEFT BLANK

DKFZphutel_17k7

group: uterus derived

DKFZphutel_17k7 encodes a novel 520 amino acid protein with weak similarity to S. Cerevisiae Fipl.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of uterus-specific genes . similarity to S. cerevisiae Fipl complete cDNA, complete eds, EST hits

Sequenced by BMFZ

Locus: unknown

Insert length: 1914 bp

Poly A stretch at pos. 1897, polyadenylation signal at pos. 1867

1 CGGACGCGTG GGCGGACGCG TGGGGCCTTC CTGGGATTGG AGTCTCGAGC 51 TTTCTTCGTT CGTTCGCCGG CGGGTTCGCG CCCTTCTCGC GCCTCGGGGC

101 TGCGAGGCTG GGGAAGGGGT TGGAGGGGGC TGTTGATCGC CGCGTTTAAG

151 TTGCGCTCGG GGCGGCCATG TCGGCCGGCG AGGTCGAGCG CCTAGTGTCG

201 GAGCTGAGCG GCGGGACCGG AGGGGATGAG GAGGAAGAGT GGCTCTATGG

251 CGATGAAAAT GAAGTTGAAA GGCCAGAAGA AGAAAATGCC AGTGCTAATC

301 CTCCATCTGG AATTGAAGAT GAAACTGCTG AAAATGGTGT ACCAAAACCG

351 AAAGTGACTG AGACCGAAGA TGATAGTGAT AGTGACAGCG ATGATGATGA

401 AGATGATGTT CATGTCACTA TAGGAGACAT TAAAACGGGA GCACCACAGT

451 ATGGGAGTTA TGGTACAGCA CCTGTAAATC TTAACATCAA GACAGGGGGA

501 AGAGTTTATG GAACTACAGG GACAAAAGTC AAAGGAGTAG ACCTTGATGC

551 ACCTGGAAGC ATTAATGGAG TTCCACTCTT AGAGGTAGAT TTGGATTCTT

601 TTGAAGATAA ACCATGGCGT AAACCTGGTG CTGATCTTTC TGATTATTTT

651 AATTATGGGT TTAATGAAGA TACCTGGAAA GCTTACTGTG AAAAACAAAA

701 GAGGATACGA ATGGGACTTG AAGTTATACC AGTAACCTCT ACTACAAATA

751 AAATTACGGT ACAGCAGGGA AGAACTGGAA ACTCAGAGAA AGAAACTGCC

801 CTTCCATCTA CAAAAGCTGA GTTTACTTCT CCTCCTTCTT TGTTCAAGAC

851 TGGGCTTCCA CCGAGCAGGA GATTACCTGG GGCAATTGAT GTTATCGGTC

901 AGACTATAAC TATCAGCCGA GTAGAAGGCA GGCGACGGGC AAATGAGAAC

951 AGCAACATAC AGGTCCTTTC TGAAAGATCT GCTACTGAAG TAGACAACAA 1001 TTTTAGCAAA CCACCTCCGT TTTTCCCTCC AGGAGCTCCT CCCACTCACC 1051 TTCCACCTCC TCCATTTCTT CCACCTCCTC CGACTGTCAG CACTGCTCCA 1101 CCTCTGATTC CACCACCGGG TTTTCCTCCT CCACCAGGCG CTCCACCTCC 1151 ATCTCTTATA CCAACAATAG AAAGTGGACA TTCCTCTGGT TATGATAGTC 1201 GTTCTGCACG TGCATTTCCA TATGGCAATG TTGCCTTTCC CCATCTTCCT 1251 GGTTCTGCTC CTTCGTGGCC TAGTCTTGTG GACACCAGCA AGCAGTGGGA 1301 CTATTATGCC AGAAGAGAGA AAGACCGAGA TAGAGAGAGA GACAGAGACA 1351 GAGAGCGAGA CCGTGATCGG GACAGAGAAA GAGAACGCAC CAGAGAGAGA 1401 GAGAGGGAGC GTGATCACAG TCCTACACCA AGTGTTTTCA ACAGCGATGA 1451 AGAACGATAC AGATACAGGG AATATGCAGA AAGAGGTTAT GAGCGTCACA 1501 GAGCAAGTCG AGAAAAAGAA GAACGACATA GAGAAAGACG ACACAGGGAG 1551 AAAGAGGAAA CCAGACATAA GTCTTCTCGA AGTAATAGTA GACGTCGCCA 1601 TGAAAGTGAA GAAGGAGATA GTCACAGGAG ACACAAACAC AAAAAATCTA 1651 AAAGAAGCAA AGAAGGAAAA GAAGCGGGCA GTGAGCCTGC CCCTGAACAG 1701 GAGAGCACCG AAGCTACACC TGCAGAATAG GCATGGTTTT GGCCTTTTGT 1751 GTATATTAGT ACCAGAAGTA GATACTATAA ATCTTGTTAT TTTTCTGGAT 1801 AATGTTTAAG AAATTTACCT TAAATCTTGT TCTGTTTGTT AGTATGAAAA 1851 GTTAACTTTT TTTCCAAAAT AAAAGAGTGA ATTTTTCATG TTAAGTTAAA 1901 AAAAAAAAAA AAAA

BLAST Results o BLAST result

Medline entries o Medlme entry Peptide information for frame 3

ORF from 168 bp to 1727 bp; peptide length: 520 Category: similarity to known protein

1 MSAGEVERLV SELSGGTGGD EEEEWLYGDE NEVERPEEEN ASANPPSGIE 51 DETAENGVPK PKVTETEDDS DSDSDDDEDD VHVTIGDIKT GAPQYGSYGT 101 APVNLNIKTG GRVYGTTGTK VKGVDLDAPG SINGVPLLEV DLDSFEDKPW 151 RKPGADLSDY FNYGFNEDTW KAYCEKQKRI RMGLEVIPVT STTNKITVQQ 201 GRTGNSEKET ALPSTKAEFT SPPSLFKTGL PPSRRLPGAI DVIGQTITIS 251 RVEGRRRANE NSNIQVLSER SATEVDNNFS KPPPFFPPGA PPTHLPPPPF 301 LPPPPTVSTA PPLIPPPGFP PPPGAPPPSL IPTIESGHSS GYDSRSARAF 351 PYGNVAFPHL PGSAPSWPSL VDTSKQWDYY ARREKDRDRE RDRDRERDRD 401 RDRERERTRE RERERDHSPT PSVFNSDEER YRYREYAERG YERHRASREK 451 EERHRERRHR EKEETRHKSS RSNSRRRHES EEGDSHRRHK HKKSKRSKEG 501 KEAGSEPAPE QESTEATPAE

BLASTP hits

Entry AF016427_4 from database TREMBL: gene: "F32D1.9"; Caenorhabditis elegans cosmid F32D1.

Score = 392, P = 1.8e-36, identities = 156/519, positives = 212/519

Entry S62454 from database PIR: hypothetical protein SPAC22G7.10 - fission yeast (Schizosaccharomyces pombe)

Score = 246, P = 2.0e-22, identities = 62/163, positives = 91/163

Entry A56545 from database PIR:

FIP1 protein - yeast (Saccharomyces cerevisiae)

Score = 186, P = 2.9e-16, identities = 56/206, positives = 92/206

Alert BLASTP hits for DKFZphutel_17k7, frame 3

TREMBLNEW :AF109907_1 product: "S164"; Homo sapiens S164 gene, partial eds; PSl and hypothetical protein genes, complete eds; and S171 gene, partial eds., N = 2, Score = 236, P = 1.5e-16

>TREMBLNEW:AF109907_1 product: "S164"; Homo sapiens S164 gene, partial eds; PSl and hypothetical protein genes, complete eds; and S171 gene, partial eds .

Length = 735

HSPs:

Score = 236 (35.4 bits), Expect = 1.5e-16, Sum P(2) = 1.5e-16 Identities = 51/120 (42%), Positives = 76/120 (63%)

Query: 383 REKDRDRERDRDRERDRDRDRERERTRERERERDHSPTPSVFNSDEERYRYREYA ER 439

REK+++RER+R+R+RDRDR +ER+R R+RER+RD S + +++R R RE + ER Sbjct: 227 REKEKERERERERDRDRDRTKERDRDRDRERDRDRDRERSS-DRNKDRSRSREKSRDRER 285

Query: 440 GYERHRASREKEERHRER-RHREKEETRHKSSRSNSRRRHESEEGDSHRRHKHKKSKRSK 498

ER R + ER RER R RE+E R + + +R E +E D++ R K ++ R K Sbjct: 286 EREREREREREREREREREREREREREREREREKDKKRDREEDEEDAYERRKLERKLREK 345

Query: 499 E 499

E Sbjct: 346 E 346

Score = 214 (32.1 bits), Expect = 4.4e-14, Sum P(2) = 4.4e-14 Identities = 50/133 (37%), Positives = 75/133 (56%)

Query: 383 REKDRDR-ERDRDRERDRDRDRERERTRERERERDHSPTPSVFNS-DEERYRYREYAERG 440

RE++R+R ER+R+RER+R+R++E+ER RERER+RD T D ER R R+ ER Sbjct: 208 RERERERREREREREREREREKEKERERERERDRDRDRTKERDRDRDRERDRDRD-RERS 266

Query: 441 YERHRASREKEERHRERRHREKEETRHKSSRSNSRRRHESEEGDSHRRHKHKKSKRSKEG 500

+R++ E+ R+R RE+E R + R R R E + R + ++ K K Sbjct: 267 SDRNKDRSRSREKSRDRE-RERERERERE-REREREREREREREREREREREREKDKKRD 324

Query: 501 KEAGSEPAPEQESTE 515

+E E A E+ E Sbjct: 325 REEDEEDAYERRKLE 339 Score = 214 (32.1 bits), Expect = 4.4e-14, Sum P(2) = 4.4e-14 Identities = 55/141 (39%), Positives = 80/141 (56%)

Query: 383 REKDRDR-ERDRDRERDRDRDRERERTRERERERDHSPTPSVFNS-DEERYRYREYAERG 440

RE++R+R ER+R+RER+R+R++E+ER RERER+RD T D ER R R+ ER Sbjct: 208 RERERERREREREREREREREKEKERERERERDRDRDRTKERDRDRDRERDRDRD-RERS 266

Query: 441 YERHR-ASREKEE-RHRER-RHREKEETRHKSSRSNSRRRHESEEGDSHRRHKHKKSKRS 497

+R++ SR +E+ R RER R RE+E R + R E E R K KK R Sbjct: 267 SDRNKDRSRSREKSRDREREREREREREREREREREREREREREREREREREKDKKRDRE 326

Query: 498 KEGKEAGSEPAPEQESTEATPA 519

++ ++A E++ E A Sbjct: 327 EDEEDAYERRKLERKLREKEAA 348

Score = 210 (31.5 bits), Expect = 1.2e-13, Sum P(2) = 1.2e-13 Identities = 59/142 (41%), Positives = 78/142 (54%)

Query: 383 REKDRDRERDRDRERDRDRDRERERTRERERERDHSPTPSVFNS DEERYRYREYAER 439

RE++RDR+RDR +ERDRDRDRER+R R+RER D + S D ER R RE ER Sbjct: 235 RERERDRDRDRTKERDRDRDRERDRDRDRERSSDRNKDRSRSREKSRDRERERERE-RER 293

Query: 440 GYERHRA-SREKE-ERHRER-RHREKEETRHKSS RSNSRRRHESEEGDSHRRH 489

ER R RE+E ER RER R REK++ R + R R+ +E R

Sbjct: 294 EREREREREREREREREREREREREKDKKRDREEDEEDAYERRKLERKLREKEAAYQERL 353

Query: 490 KHKKSKRSKEGKEAGSEPAPEQE 512

K+ + + K+ +E E E+E Sbjct: 354 KNWEIRERKKTREYEKEAEREEE 376

Score = 205 (30.8 bits), Expect = 4.4e-13, Sum P(2) = 4.4e-13 Identities = 59/149 (39%), Positives = 83/149 (55%)

Query: 372 DTSKQWDYYARREKDRDR—ERDRDRERDRDRDRERERTRERERERDHSPTPSVFNSDEE 429

+ K+ + R++DRDR ERDRDR+R+RDRDR+RER+ +R ++R S S D E Sbjct: 228 EKEKERERERERDRDRDRTKERDRDRDRERDRDRDRERSSDRNKDRSRSREKS RDRE 284

Query: 430 RYRYREYAERGYERHRA-SREKE-ERHRER-RHREKEETRHKSS RSNSRRRHE 479

R R RE ER ER R RE+E ER RER R REK++ R + R R+

Sbjct: 285 RERERE-REREREREREREREREREREREREREREKDKKRDREEDEEDAYERRKLERKLR 343

Query: 480 SEEGDSHRRHKHKKSKRSKEGKEAGSEPAPEQE 512

+E R K+ + + K+ +E E E+E Sbjct: 344 EKEAAYQERLKNWEIRERKKTREYEKEAEREEE 376

Score = 202 (30.3 bits), Expect = 9.6e-13, Sum P(2) = 9.6e-13 Identities = 49/117 (41%), Positives = 70/117 (59%)

Query: 383 REKDRDRERDRDRERDRDRDRERERTRERERERDHSPTPSVFNSDEERYRYREYAERGYE 442

REK RDRER+R+RER+R+R+RERER RERERER+ D++R R E E YE

Sbjct: 277 REKSRDRERERERERERERERERERERERERERERERERER-EKDKKRDR-EEDEEDAYE 334

Query: 443 RHRASREKEERHRERRHREKEETRHKSSRSNSRR-RHESEEGDSHRRHKHKKSKRSKE 499

R + E++ R +E ++E+ + R +R E+E + RR K++KR KE Sbjct: 335 RRKL—ERKLREKEAAYQERLKNWEIRERKKTREYEKEAEREEERRREMAKEAKRLKE 390

Score = 183 (27.5 bits), Expect = 1.2e-10, Sum P(2) = 1.2e-10 Identities = 52/141 (36%), Positives = 79/141 (56%)

Query: 372 DTSKQWDYY-ARREKDRDR-ERDRDRERDRDRDRERERTRERERERDHSPTPSVFNSDEE 429

DT K+ + ++EK+R E++R RER+R+R+RERER RERERER+ ++E

Sbjct: 178 DTHKKLEEEKGKKEKERQEIEKER-RERERERERERER-RERERERERER EREKE 230

Query: 430 RYRYREYAERGYERHRASREKEERHRER RHREKEETRHKSSRSNSRRRHESEEGDSH 486

+ R RE ER +R R +R RER R RE+ R+K RS SR + E + Sbjct: 231 KERERE-RERDRDRDRTKERDRDRDRERDRDRDRERSSDRNKD-RSRSREKSRDRERERE 288

Query: 487 RRHKHKKSKRSKEGKEAGSEPAPEQE 512

R + ++ + + +E E E+E Sbjct: 289 RERERERERERERERERERERERERE 314

Score = 171 (25.7 bits), Expect = 2.5e-09, Sum P(2) = 2.5e-09 Identities = 49/150 (32%), Positives = 78/150 (52%)

Query: 383 REKDRDRERDRDRERDRDRDRERERTRERERERDHSPTPSVFNSDEERYRYREYAERGYE 442

RE++R+RER+R+RER+R+R+RERER RERERER+ +E+ Y R+ + E

Sbjct: 285 REREREREREREREREREREREREREREREREREKDKKRDREEDEEDAYERRKLERKLRE 344

Query: 443 RHRASREK EERHRERRHR EKEETRHKSSRSNSRRRHES-EEGDSHRRH-KH 491

+ A +E+ ER + R + E+EE R + ++R E E+ D R K+ Sbjct: 345 KEAAYQERLKNWEIRERKKTREYEKEAEREEERRREMAKEAKRLKEFLEDYDDDRDDPKY 404 Query: 492 KKSKRSKEGKEAGSEPAPEQESTE 515

+K R +E + E ++E E Sbjct: 405 YRGSALQKRLRDREKEMEADERDRKREKEE 434

Score = 162 (24.3 bits), Expect = 2.4e-08, Sum P(2) = 2.4e-08 Identities = 45/141 (31%), Positives = 74/141 (52%)

Query: 372 DTSKQWDYYARREKDRDRERDRDRERDRDRDRERERTRERERERDHSPTPSVFNSDEERY 431

+ SK D + + E+++ ++ +E +++R RERER RERERER + ER

Sbjct: 172 EISKFRDTHKKLEEEKGKKEKERQEIEKER-RERERERERERERRERERER--ERERERE 228

Query: 432 RYREYAERGYERHRASREKEERHRER-RHREKEETRHKSSRSNSRRRHESEEGDSHRRHK 490

+ +E ER ER R +ER R+R R R+++ R +SS N R E+ R + Sbjct: 229 KEKE-RERERERDRDRDRTKERDRDRDRERDRDRDRERSSDRNKDRSRSREKSRDRERER 287

Query: 491 HKKSKRSKEGKEAGSEPAPEQE 512

++ +R +E +E E E+E Sbjct: 288 ERERERERE-RERERERERERE 308

Score = 137 (20.6 bits), Expect = 1.2e-05, Sum P(2) = 1.2e-05 Identities = 48/152 (31%), Positives = 68/152 (44%)

Query: 364 APSWPSLVDTSKQWDYYARREKDRDR-ERDRDRERDRDRDRERERTRERERERDHSPTPS 422

AP P + T + + E RD R+ + RD + E E+ + +E+ER Sbjct: 143 APLIPYPLITKEDINAIEMEEDKRDLISREISKFRDTHKKLEEEKGK-KEKERQEIEKER 201

Query: 423 VFNSDEERYRYREYAERGYERHRA-SREKE-ERHRER-RHREKEETRHKS-SRSNSRRRH 478

+ ER R RE ER ER R REKE ER RER R R+++ T+ + R R R Sbjct: 202 R-ERERERERERERREREREREREREREKEKERERERERDRDRDRTKERDRDRDRERDRD 260

Query: 479 ESEEGDSHRRHKHKKSKRSKEGKEAGSEPAPEQE 512

E S R +S+ +E E E+E Sbjct: 261 RDRERSSDRNKDRΞRΞREKSRDRERERERERERE 294

Score = 126 (18.9 bits), Expect = 1.8e-04, Sum P(2) = 1.8e-04 Identities = 41/149 (27%), Positives = 66/149 (44%)

Query: 375 KQWDYYARREKDRDRERDRDRERDRDRDRERERTRERERERDHSPT PSVFNSD--EE 429

K W+ R+K R+ E++ +RE +R R+ +E R +E D+ P + ++ Sbjct: 354 KNWEI-RERKKTREYEKEAEREEERRREMAKEAKRLKEFLEDYDDDRDDPKYYRGSALQK 412

Query: 430 RYRYREYAERGYERHRASREKEERHRERR HREKEETRHKSSRSNSRRRHEΞ—E 481

R R RE ER R REKEE R+ H + + + + RRR + Sbjct: 413 RLRDREKEMEADERDR-KREKEELEEIRQRLLAEGHPDPDAELQRMEQEAERRRQPQIKQ 471

Query: 482 EGDSHRRHKHKKSKRSKEGKEAGSEPAPEQE 512

E +S + K+ K K + E PEQ+ Sbjct: 472 EPESEEEEEEKQEKEEKREEPMEEEEEPEQK 502

Score = 124 (18.6 bits), Expect = 3.0e-04, Sum P(2) = 3.0e-04 Identities = 41/141 (29%), Positives = 65/141 (46%)

Query: 380 YARREKDRD-RERDRDRERDRDRDRERERTRERERERDHSPTPSVFNSDEERYRYREYAE 438

Y R K+ + RER + RE +++ +RE ER RE +E + + D++R + Y Sbjct: 349 YQERLKNWEIRERKKTREYEKEAEREEERRREMAKEAKRLKE-FLEDYDDDRDDPKYYRG 407

Query: 439 RGYERHRASREKEERHRER-RHREKEETRHKSSRSNSRRRHESEEGDSHRRHKHKKSKRS 497

++ REKE ER R REKEE R + H + + R + + +R Sbjct: 408 SALQKRLRDREKEMEADERDRKREKEELEEIRQRLLAEG-HPDPDAELQRMEQEAERRRQ 466

Query: 498 KEGKEAGSEPAPEQESTEATPAE 520

+ K+ EP E+E E E Sbjct: 467 PQIKQ EPESEEEEEEKQEKE 486

Score = 121 (18.2 bits), Expect = 6.2e-04, Sum P(2) = 6.2e-04 Identities = 43/149 (28%), Positives = 67/149 (44%)

Query: 364 APSWPSLVDTSKQWDYYARREKDRDR-ERDRDRERDRDRDRERERTRERERERDHSPTPS 422

AP P + T + + E RD R+ + RD + E E+ + +E+ER Sbjct: 143 APLIPYPLITKEDINAIEMEEDKRDLISREISKFRDTHKKLEEEKGK-KEKERQEIEKE- 200

Query: 423 VFNSDEERYRYREYAERGYERHRASREKEERHRERRHREKEETRHKSSRSNSRRRHESEE 482

+ ER R RE R ER R RE+E + R RE+E R + R+ R R E Sbjct: 201 —RRERERERERERERRERERER-EREREREKEKERERERERDRDRD-RTKERDRDRDRE 256

Query: 483 GDSHRRHKHKKSKRSKEGKEAGSEPAPEQE 512

D R + + S R+K+ + E + ++E Sbjct: 257 RDRDR-DRERSSDRNKD-RΞRSREKSRDRE 284

Score = 105 (15.8 bits), Expect = 3.1e-02, Sum P(2) = 3.1e-02 Identities = 25/73 (34%), Positives = 33/73 (45%)

Query: 4₂8 EERYRYREYAERGYERHRASREKE-ERHRERRHREKEETRHKSSRSNSRRRHESEEGDSH 486

EE +E + E+ R RE+E ER RERR RE+E R + R E E Sbjct: 184 EEEKGKKEKERQEIEKERRERERERERERERREREREREREREREKEKERERERERDRDR 243

Query: 487 RRHKHKKSKRSKE 499

R K + R +E Sbjct: 244 DRTKERDRDRDRE 256

Score = 105 (15.8 bits), Expect = 3.1e-02, Sum P(2) = 3. le-02 Identities = 31/87 (35%), Positives = 45/87 (51%)

Query: 382 RREKDRDRERDRDRERDRDRDRER-ERTRERERERDHSPTPSVFNSDEERYRYREYAERG 440

+R +DR++E + D ERDR R++E E R+R H P P D E R + AER Sbjct: 412 KRLRDREKEMEAD-ERDRKREKEELEEIRQRLLAEGH-PDP DAELQRMEQEAERR 464

Query: 441 YERHRASREKEERHRERRHREKEETRHK 468

+ + +E E E +EKEE R + Sbjct: 465 -RQPQIKQEPESEEEEEEKQEKEEKREE 491

Score = 46 (6.9 bits), Expect = 1.5e-16, Sum P(2) = 1.5e-16 Identities = 13/49 (26%), Positives = 21/49 (42%)

Query: 54 AENGVPKPKVTETEDDSDSDSDDDEDDVHVTIGDIKTGAPQYGSYGTAP 102

A NG +P+ +D+ D + D + G 1+ +Y S AP Sbjct: 70 ASNGNARPETVTNDDEEALDEETKRRDQMIK-GAIEVLIREYSSELNAP 117

Score = 46 (6.9 bits), Expect = 1.8e-04, Sum P(2) = 1.8e-04 Identities = 14/53 (26%), Positives = 21/53 (39%)

Query: 30 ENEVERPEEENASANPPSGIEDETAENGVPKPKVTETEDDSDSDSDDDEDDVH 82

+ E ER E E E E + + E E D D ++DE+D +

Sbjct: 282 DRERERERERERERERERERERER-EREREREREREREKDKKRDREEDEEDAY 333

Score = 44 (6.6 bits), Expect = 2.0e-13, Sum P(2) = 2.0e-13 Identities = 13/60 (21%), Positives = 21/60 (35%)

Query: 20 DEEEEWLYGDENEVERPEEENASANPPSGIEDETAENGVPKPKVTETEDDSDSDSDDDED 79

++E + + + E ER E + E K + E E D D D + D

Sbjct: 191 EKERQEIEKERRERERERERERERREREREREREREREKEKERERERERDRDRDRTKERD 250

Pedant information for DKFZphutel_17k7, frame 3

Report for DKFZphutel_17k7.3

[LENGTH] 520

[MW] 58375.30

[pi] 5.41

[HOMOL] PIR:S62454 hypothetical protein SPAC22G7.10 - fission yeast

(Schizosaccharomyces pombe) 3e-18

[FUNCAT] 04.05.05 mrna processing (5'-end, 3'-end processing and mrna degradation) [S. cerevisiae, YJR093c] 2e-13

[FUNCAT] 3 300..1100 nnuucclleeaarr oorrggaanniizzaattiioonn [Ξ. cerevisiae, YJR093c] 2e-13

[PROSITE] MYRISTYL 9

[PROSITE] AMIDATION 1

[PROSITE] CK2 PHOSPHO SITE 18

[PROSITE] TYR PHOSPHO SITE 2

[PROSITE] PKC PHOSPHO SITE 12

[PROSITE] ASN GLYCOSYLATION 2

[KW] Alpha Beta

[KW] LOW COMPLEXITY 35. .00 %

SEQ MSAGEVERLVSELΞGGTGGDEEEEWLYGDENEVERPEEENASANPPSGIEDETAENGVPK SEG xxxxxxxxxx

PRD cccchhhhhhhhcccccccccccccccccccccccccccccccccccccccccccccccc

SEQ PKVTETEDDSDSDSDDDEDDVHVTIGDIKTGAPQYGSYGTAPVNLNIKTGGRVYGTTGTK SEG ... xxxxxxxxxxxxxxxxx

PRD cceeeecccccccccccccceeeeeccccccccccccccccceeeeeecccceeeccccc

SEQ VKGVDLDAPGSINGVPLLEVDLDSFEDKPWRKPGADLSDYFNYGFNEDTWKAYCEKQKRI SEG

PRD ceeeccccccccccceeeeccccccccccccccccccccccccccccchhhhhhhhhhhh

SEQ RMGLEVIPVTSTTNKITVQQGRTGNSEKETALPSTKAEFTSPPSLFKTGLPPΞRRLPGAI SEG PRD hhhheeeeeccccceeeeeeecccccccccccccceeeeccccceeeecccccccccccc

SEQ DVIGQTITISRVEGRRRANENSNIQVLSERSATEVDNNFSKPPPFFPPGAPPTHLPPPPF

SEG xxxxxxxxxxxxxxxxxxx

PRD ccccceeeeeecccccccccccceeecccccccccccccccccccccccccccccccccc

SEQ LPPPPTVSTAPPLIPPPGFPPPPGAPPPSLIPTIESGHSSGYDSRSARAFPYGNVAFPHL

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccceeeccc

SEQ PGSAPSWPSLVDTSKQWDYYARREKDRDRERDRDRERDRDRDRERERTRERERERDHSPT

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx ....

PRD ccccccccceeeccccchhhhhhhhhhccccccccccccccchhhhhhhhhhhhcccccc

SEQ PSVFNSDEERYRYREYAERGYERHRASREKEERHRERRHREKEETRHKSSRSNSRRRHES

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD cccccccchhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccc

SEQ EEGDSHRRHKHKKSKRSKEGKEAGΞEPAPEQESTEATPAE

SEG xx .. xxxxxxxxxxxxxx

PRD cccccccccccccccccccccccccccccccccccccccc

Prosite for DKFZphutel_17k7.3

PS00001 40->44 ASN_GLYCOSYLATION PDOC00001 PS00001 278->282 ASN_GLYCOSYLATION PDOC00001 PS00005 169->172 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 193->196 PKC_PHOSPHO_SITE PDOC00005 PS00005 206->209 PKC_PHOSPHO_SITE PDOC00005 PS00005 214->217 PKC_PHOSPHO_SITE PDOC00005 PS00005 233->236 PKC_PHOSPHO_SITE PDOC00005 PS00005 268->271 PKC_PHOSPHO_SITE PDOC00005 PS00005 346->349 PKC_PHOSPHO_SITE PDOC00005 PS00005 373->376 PKC_PHOSPHO_SITE PDOC00005 PS00005 469->472 PKC_PHOSPHO_SITE PDOC00005 PS00005 474->477 PKC_PHOSPHO_SITE PDOC00005 PS00005 485->488 PKC_PHOSPHO_SITE PDOC00005 PS00005 494->497 PKC_PHOSPHO_SITE PDOC00005 PS00006 2->6 CK2_PHOSPHO_SITE PDOC00006 PS00006 17->21 CK2_PHOSPHO_SITE PDOC00006 PS00006 47->51 CK2_PHOSPHO_SITE PDOC00006 PS00006 64->68 CK2_PHOSPHO_SITE PDOC00006 PS00006 66->70 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 70->74 CK2_PHOSPHO_SITE PDOC00006 PS00006 72->76 CK2_PHOSPHO_SITE PDOC00006 PS00006 74->78 CK2_PHOSPHO_SITE PDOC00006 PS00006 84->88 CK2_PHOSPHO_SITE PDOC00006 PS00006 144->148 CK2_PHOSPHO_SITE PDOC00006 PS00006 206->210 CK2_PHOSPHO_SITE PDOC00006 PS00006 215->219 CK2_PHOSPHO_SITE PDOC00006 PS00006 250->254 CK2_PHOSPHO_SITE PDOC00006 PS00006 271->275 CK2_PHOSPHO_SITE PDOC00006 PS00006 273->277 CK2_PHOSPHO_SITE PDOC00006 PS00006 340->344 CK2_PHOSPHO_SITE PDOC00006 PS00006 369->373 CK2_PHOSPHO_SITE PDOC00006 PS00006 426->430 CK2_PHOSPHO_SITE PDOC00006 PS00007 434->442 TYR_PHOSPHO_SITE PDOC00007 PS00007 152->161 TYR_PHOSPHO_SITE PDOC00007 PS00008 15->21 MYRISTYL PDOC00008 PS00008 96->102 MYRISTYL PDOC00008 PS00008 115->121 MYRISTYL PDOC00008 PS00008 130->136 MYRISTYL PDOC00008 PS00008 154->160 MYRISTYL PDOC00008 PS00008 229->235 MYRISTYL PDOC00008 PS00008 244->250 MYRISTYL PDOC00008 PS00008 289->295 MYRISTYL PDOC00008 PS00008 362->368 MYRISTYL PDOC00008 PS00009 253->257 AMIDATION PDOC00009

(No Pfam data available for DKFZphutel_17k7.3) DKFZphutel_18cl2

group: uterus derived

DKFZphutel_18cl2 encodes a novel 378 amino acid protein nearly identical to human WUGSC : H_DJ0872F07.1 protein.

The novel protein has an additional N-terminal domain, which is not present in

WUGSC : H_DJ0872F07.1.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of uterus-specific genes . nearly identical to human WUGSC : H_DJ0872F07.1 protein on genomic level encoded by AC004537, 10 exons the predicted protein sequence AC004537_1 is only partialy o.k. first exon wasn't predicted there are additional exons predicted (BLASTX/EST-BLAST shows that the cDNA is only party spliced) intron -1216-3540//-3577-5059

Sequenced by AGOWA

Locus: map="7q31"

Insert length: 6005 bp

Poly A stretch at pos. 5980, polyadenylation signal at pos. 5968

1 AGCGGGTGCT GCTAGCGGAG GCGCCATATT GGAGGGGACA AAACTCCGGC 51 GACAGCGAGT GACACAAATA AACCCCTGGA CCCCCTTGTT CCCTCAGCTC

101 TAAGGGCCGC GATGTTGTAC CTAGAAGACT ATCTGGAAAT GATTGAGCAG

151 CTTCCTATGG ATCTGCGGGA CCGCTTCACG GAAATGCGCG AGATGGACCT

201 GCAGGTGCAG AATGCAATGG ATCAACTAGA ACAAAGAGTC AGTGAATTCT

251 TTATGAATGC AAAGAAAAAT AAACCTGAGT GGAGGGAAGA GCAAATGGCA

301 TCCATCAAAA AAGACTACTA TAAAGCTTTG GAAGATGCAG ATGAGAAGGT

351 TCAGTTGGCA AACCAGATAT ATGACTTGGT AGATCGACAC TTGAGAAAGC

401 TGGATCAGGA ACTGGCTAAG TTTAAAATGG AGCTGGAAGC TGATAATGCT

451 GGAATTACAG AAATATTAGA GAGGCGATCT TTGGAATTAG ACACTCCTTC

501 ACAGCCAGTG AACAATCACC ATGCTCATTC ACATACTCCA GTGGAAAAAA

551 GGAAATATAA TCCAACTTCT CACCATACGA CAACAGATCA TATTCCTGAA

601 AAGAAATTTA AATCTGAAGC TCTTCTATCC ACCCTTACGT CAGATGCCTC

651 TAAGGAAAAT ACACTAGGTT GTCGAAATAA TAATTCCACA GCCTCTTCTA

701 ACAATGCCTA CAATGTGAAT TCCTCCCAAC CTCTGGGATC CTATAACATT

751 GGCTCGTTAT CTTCAGGAAC TGGTGCAGGG GCAATTACCA TGGCAGCTGC

801 TCAAGCAGTT CAGGCTACAG CTCAGATGAA GGAGGGACGA AGAACATCAA

851 GTTTAAAAGC CAGTTATGAA GCATTTAAGA ATAATGACTT TCAGTTGGGA

901 AAAGAATTTT CAATGGCCAG GGAAACAGTT GGCTATTCAT CATCTTCGGC

951 ACTTATGACA ACATTAACAC AGAATGCCAG TTCATCAGCA GCCGACTCAC 1001 GGAGTGGTCG AAAGAGCAAA AACAACAACA AGTCTTCAAG CCAGCAGTCA 1051 TCATCTTCCT CCTCCTCTTC TTCCTTATCA TCGTGTTCTT CATCATCAAC 1101 TGTTGTACAA GAAATCTCTC AACAAACAAC TGTAGTGCCA GAATCTGATT 1151 CAAATAGTCA GGTTGATTGG ACTTACGACC CAAATGAACC TCGATACTGC 1201 ATTTGTAATC AGGTAAAAGT CTGTTATATC TATAAAAGTA TAATCTGAAT 1251 AAACTAGAAG GAAGAGAACT ATTTCATTTT TAAGCACTTT TTTAAACTCA 1301 CTTAAAATAC CTTTGCTTTA TTTGTATACT TTTCTCCCCC TTCTTACAAA 1351 AGTGACATTT GCTGTAAATA CTGAGTATAA AGAAAAATGT TACCCATAAT 1401 CCTAGCCCTC AGATACAACC TGTAACTAAA CATTTTTGGT ATACCACTAC 1451 CATATACCTC ATGTGCACAT TGGCTGCCTT AATAAAATAC AACAGACTGG 1501 GTAGCTTAAA CAACAGAAAA TAATTTTCTC ACAGGTATGA AGGCTGGGAA 1551 GTCCAAGATC AAGGTGTCCA CTGACTCAGT TCTGGAGGAG GGCTCCCTTC 1601 CTAGATGGAG ACTGCTGCCT TCTCACCGGG TCCTCACATG ATAGAGGGAG 1651 AAAGAGTGTG CTCTGGTGTC TTTTCTTATA AGGGCACCAG CCTTGTCAGA 1701 GTAGGACCCC ACTCTATGAC CTCATTTAAC CTTTACCACC TCCTCACAGG 1751 CCCTGTTTCC AATTATAGTC ACGTTGGGGG TTAGGGCTTC AACATATGAT 1801 TTTGAGACAT AAGCTTGCAT TTCATAACAC GTGTCTATGC AGATTTGCAC 1851 ATGCATGTGT GTATAAGTTT GTCAGTAGGA ACCACAGTGT ATACTTTCTT 1901 GTTACTGGCT TTTTTCTCTA AATCAGGTAT ACCGAACATG ATTTTTCTTT 1951 AAGATCATAT TTTTAATTTT CACATAGTTA TCTCTTATGC CATCCAGTGT 2001 AGTTTTCTTA ACCAATACCT AGCTATAGAT TATATTAGTG GTTTTAATTT 2051 GTTTGAAATT AGGGATAATA TTACGATAGG CATTTTTTAA ATGTAATCCA 2101 TTTTATACAT CTAATTTCTT GGATAATCTT TTAGAAATAA AATTAGGCTG 2151 TAAATATTTG ACAGACACCA AAATATATTT TCTAGAAATT TATTACCAAA 2201 AATTAATAAA CATACCGGTT TACTAAACCC TGTCCAACAC TGGATATTAT 2251 TTTCTTTTAA AAACTAAGTA CCAATTTGGT AGTTTTATAT TATGATTGTT 2301 TTAAATACAC TAGTATTATT GAAGTTGGAC ATTTTTTGAC CATTTTTGTT 2351 TTTTACATTA TGAATCGACT CCTAATGGTG TCGGCTGATT TTTCTATTGT 2401 TTTTGTTATG TACTCTAAAT ATTTGCTTGA TTTAGTTTTT TAAAAATAAT

2451 TCTAAAATTT TAATTTTATG TAGTTATGAC TGTTAATTTT TTTTTATGAA

2501 GCAAGCCATG GATTATATAC TTAGAAGGGC TTTCTCTTTG GCTCTTCTTT

2551 CTACAAAAAA TTGTCTTGTA TAATATTTTC TCCTAGTTTT TATATGGTTT

2601 TGTCTAGTTC TTTGCATGCT TCAGTTTCTT CACATTTAAG ACTTAGTCTA

2651 TCAGCAGATT ATTGTGTCTA ACAGTATGAG TTGCCAGTCT GATTTTTAAA

2701 AATTTTAACA ATTTGTTAGC TGTTCCACTA TCACCCGATA AACATTTTTC

2751 AGTACAAATG ATAGAAAAGC ATATCCTGTA TCCTGACAAC AAAAGTAGAT

2801 TACTTGCAAA AGAACAAAAT CAGACTGAAC CTAGAGTTTT CCTCTGTAAC

2851 ACTAAAAAAC TAGAAGGTGA TGGAATATGT CTGTAGAGCT TTCAGGGAAA

2901 AATTAAGAGC CCCCAAAAAC TTGATATTCA GAGAAGTTAT TTCTCTGCAT

2951 AGGACCATGT AAATATATTT TCACTCATGC AGAGAATCAG AAGATATGCC

3001 ATCTAGTTAA TCCTGTCTGA AAAATTATTC AATCCACTGA GAACTTCAGT

3051 GAACTCAAGA ATTAGCAAGT TATGCCCTAA AGTGCTGGTG ATGAAGAGCA

3101 AAAGAAAAAT GAGAAAGGAC ATAAAATAGA TAAGTTTAGA AGTTTCAAGG

3151 AAGGAGACTA TTAATTGCAA AAATATATAT GACCTAATGT GACCCAAGAA

3201 GTAAAAACTT TCAGTAAGTA AATAATCAAG AAAGGAACTT AAAATTTTTA

3251 CAATAAGAAC TACCCAGAAA GATGACTCCT TCATCCGGGT GATTTATATG

3301 TCAAGTTCTT CCAGACTTCT GAAGGGCAGA TAATTCCTGT GCATTTCTTC

3351 CCACCCTTGC CCCACCCTGC CCAAAAGAGT ATTTCAGGAA AAAATTATTA

3401 TACCTTGATT CTCAATGTAA TTGTATATTC AGTGTATTTC CCTTTATTTT

3451 CCAGCAGTAT CATACATAAA CAGTTAATTG GTATCTAGGT GTTTGTTACA

3501 TAGTCATAAT AAAGACATTT AATTTTTTTT AACTAGGTAT CTTATGGTGA

3551 GATGGTGGGA TGTGATAACC AAGATGTAAG TATTACATTT TTCTATTTAG

3601 GAATGAAAAA AATCACAGGT TGTTATTACT TGAATATTTG TCTTATTTGC

3651 TGTATGGTTT GGTCTAAGAA AACAGGTTTG CAGGTATATT AGTTATGTTA

3701 TGCTAATGCT AGAATATTCC TCTTCAAAAT AGGGTAGTGT CCCTTAATGT

3751 GTTCCCTATT TTAATTTTTA AAGCTAATTT TATGGTTTTA TGTGCAGATT

3801 GTCTCAGAAG TGTTATGTTG TATGAAAATT ATAAATACCC TCCTTTCCCT

3851 TTACTAAAAA ATACTGTGTT TACTAGAATC CAGTTCATTT ATCACATTGA

3901 AGAAATGGAA TTTTAAAACA ATTCATTCTT TCAGGCTGCA CCGTGCTAAA

3951 GTGAAGGGTG GGATAATTGA GGATCTAATG TGAGATTATC TTCCTCTCAT

4001 GAGTATAATA TTTTTTCCTG TACTCTGCAG GTGTCAGCTG ATAAGAGCCA

4051 CCCCTGATCT AAAAAGTAAA GGAAATTTGA AAGGAAGGAA TTCTTGGTTT

4101 TTAGGAGACT TAATTTTAGT TAGAGATACG TTTTTTATTC AATACTGAGA

4151 ATATTGTTGT CTAGTAATTT TGACTCCCTC CTTATTTAGT AGTGACAGGA

4201 TCCTAAGATT AACAAGAGTT TTAAATTTGT AAAACAATCT GAAGATTGAG

4251 GGAGCTGGCT AGGTGCATTA AAATGTGTAC TTTTCCTAGA CCTGATAGGG

4301 TTACAGCAAC ATGCTCACGT AGATTGGGAC AGAGCCTCCT TCTGTTTCCC

4351 TGTCTAGAAT CCCTTGTAGG CTGTTTGTGG TTGTTGCAAA AACAATATTG

4401 CCCAACCATT TCAAGAACAT CACTGTAAAC TCTTCTGGGG CAGTTAGTGA

4451 AAATGATGAA TGAGATTTCT ATGAGTACCA GCATCATGCT TCTCTGATTC

4501 TTCTTATTCC CAGTTGTGCT CTTCTGAGTG CTAAGACTTT CATGAAAGAG

4551 TTTTCTGCTT AATATGTTTC AAAGAGGAAT AATTTTTCTC TACATTTCAA

4601 GGAATAGAAA CACCCACGTA GGAAATGCAG GGCATAAGAC ATAAATTAAT

4651 GTCTTTAATT ACAATCAGCT TATTCTACTT TATGAGACAG CAAATAAGGC

4701 TGACTATTAA ATAAAATCTT AAGTTATATT TACCTTCTAC ATAGAAGATT

4751 CATCCCACTT CTTTTTGCCC TTGAAAGCTG AAAACTAGTG AATTTTCATT

4801 CATTAGGATG AGGGGACTAG ATTACATGGA CCTCAGGATT CTTGAAGATG

4851 CATAATTTTT CTGTGCCTTC ATTTCCTCAT TCCTGAAGCT TATCATTTAG

4901 TCTAAATGAT GTCTAAATAA TCTAGATCTA AAAATTCTGA TGTCACACAT

4951 CTAATTATTG TTAAATTAAA TGGATTATTC AGTCTCCTGA GCATATTTTA

5001 ATATACTCTC TTGTCTTCAG AAGTACTGAA AACTTGTTTT TTGCAATTTT

5051 GCTTTCTAGT GCCCTATAGA ATGGTTCCAT TATGGCTGCG TTGGATTGAC

5101 AGAGGCACCA AAAGGCAAAT GGTACTGTCC ACAGTGCACT GCTGCAATGA

5151 AGAGAAGAGG CAGCAGACAC AAATAAAGGT GGTCCTTTTG TTTGATGAAG

5201 AAATAAACTT CAGCTGAAGA TTTTATATAG GACTTTAAAA AGAAGAGAAG

5251 AGAAAGAAGA AACAATGCAT TTCCAGGCAA CCACTTAAAG GATTTACATA

5301 GACAATCCTA TAAGATCTTG AACTTGAATT TTATGGGTTG TATTTTAATA

5351 ATGTAAGTAA ATTATTTATG CACTCCTGGT GTGCTATGAA TATTATTCCA

5401 GTTAGCCTTG GATTATTTCA GTGGCCAACA TATGCAGACA TTTGTACTCC

5451 TCAACCATTT TCTCAAAGTA ATGGGCATTC TATGATTTAG ACTTCAAGGA

5501 ATTCCAATGA TGAAGATTTT AAGGAAAGTA TTTTATATTC AACAGGTATA

5551 TTCTGCTGCA TGTACTGTAC TCCAGAGCTG TTATGTAACA CTGTATATAA

5601 ATGGTTGCAA AAAAAAAAAA AAGTCAGTGC TTCTAAAAAG AATTTAAGAT

5651 AATGGTTTTT AAAATGCCTT TATAATAAGC TTTGTTTCTT TGTGAAACTA

5701 ATTCAGCAGG CTGAAGGAAA TGGTTCATGT GATAATGTGG GCTGGTATCC

5751 TCTAGAGTAC CTGGGTACAT AAACAGAAAC TCCTGTAGGT AAAAAGTAAT

5801 TTGTGCCATT AGTCTTTCTA TGTTTCTGCA TCCAGATAGA GTGCAGTTCA

5851 TGAGGGAGGG GGCGGGGGAC TGAAGGGGAA AGGGCGTTAA AGTGATACAT

5901 TTTTATACCA AATGTGTTTA TTTTTTTGTG CAAGTAATCC TTAAAATTGC

5951 AATTGTATTA GGTGTTAAAA TAAAGTTTTT AAAAAATTAA AAAAAAAAAA

6001 AAAAA

BLAST Results

Entry HSG20547 from database EMBL: HSG20547| human STS A005W09. Length = 154 Minus Strand HSPs:

Score = 770 (115.5 bits), Expect = 2.9e-26, P = 2.9e-26

Identities = 154/154 (100%)

Medline entries

98101645:

The candidate tumour suppressor p33INGl cooperates with p53 in cell growth control.

Peptide information for frame 1

ORF from 112 bp to 1245 bp; peptide length: 378 Category: similarity to known protein

1 MLYLEDYLEM IEQLPMDLRD RFTEMREMDL QVQNAMDQLE QRVSEFFMNA

51 KKNKPEWREE QMASIKKDYY KALEDADEKV QLANQIYDLV DRHLRKLDQE

101 LAKFKMELEA DNAGITEILE RRSLELDTPS QPVNNHHAHS HTPVEKRKYN

151 PTSHHTTTDH IPEKKFKΞEA LLSTLTSDAS KENTLGCRNN NSTASSNNAY

201 NVNSSQPLGS YNIGSLSSGT GAGAITMAAA QAVQATAQMK EGRRTSSLKA

251 SYEAFKNNDF QLGKEFSMAR ETVGYSSSSA LMTTLTQNAS SSAADSRSGR

301 KSKNNNKSSS QQSSSSSSSS SLSSCSSSST VVQEISQQTT VVPESDSNSQ

351 VDWTYDPNEP RYCICNQVKV CYIYKSII

BLASTP hits

Entry AF044076_1 from database TREMBL:

"INGl"; product: "candidate tumor suppressor p33INGl"; Homo sapiens candidate tumor suppressor p33INGl (INGl) mRNA, complete eds. Homo sapiens (human)

Length = 279

Score = 162 (57.0 bits), Expect = l.le-09, P = l.le-09

Identities = 48/183 (26%), Positives = 92/183 (50%)

Entry AC004537_1 from database TREMBL: gene: "WUGSC :H_DJ0872F07.1"; Homo sapiens PAC clone DJ0872F07 from

7q31, complete sequence.

Score = 1814, P = 3.7e-187, identities = 358/358, positives = 358/356

Entry CEY51H1A_1 from database TREMBL: gene: "Y51H1A.4"; Caenorhabditis elegans cosmid Y51H1A

Score = 213, P = 3.7e-15, identities = 37/123, positives = 82/123

Alert BLASTP hits for DKFZphutel_18cl2, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphutel_18cl2, frame 1

Report for DKFZphutel_18cl2.1

[LENGTH] 378

[MW] 42275.72

[pi] 5.72

[HOMOL] TREMBL:AC004537_1 gene: "WUGSC :H_DJ0872F07.1"; Homo sapiens PAC clone DJ0872F07 from 7q31, complete sequence, le-157

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YHR090c] 8e-05

[FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YNL097c] 2e-04

[PROSITE] MYRISTYL 3

[PROSITE] AMIDATION 2

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 4

[PROSITE] PROKAR_LIPOPROTEIN 1

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE 3

[PROSITE] ASN_GLYCOSYLATION 5

[KW] All_Alpha

[KW] LOW COMPLEXITY 20.63 % [KW] COILED COIL 7.94 %

SEQ MLYLEDYLEMIEQLPMDLRDRFTEMREMDLQVQNAMDQLEQRVSEFFMNAKKNKPEWREE SEG PRD ccchhhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccchhhhh COILS

SEQ QMASIKKDYYKALEDADEKVQLANQIYDLVDRHLRKLDQELAKFKMELEADNAGITEILE SEG PRD hhhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccchhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ RRSLELDTPSQPVNNHHAHSHTPVEKRKYNPTSHHTTTDHIPEKKFKSEALLSTLTSDAS SEG PRD hhccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhhcccc COILS

SEQ KENTLGCRNNNSTASSNNAYNVNSSQPLGΞYNIGSLSSGTGAGAITMAAAQAVQATAQMK SEG xxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxx.. PRD cccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhhh COILS

SEQ EGRRTSSLKASYEAFKNNDFQLGKEFSMARETVGYSSSSALMTTLTQNASSSAADSRSGR SEG xxxxxxxxxxxx PRD hccccccccchhhhhhccccccccccccccccccccccceeeeecccccccccccccccc COILS

SEQ KSKNNNKSSSQQSSSSSSSSSLSSCSSSSTVVQEISQQTTVVPESDSNSQVDWTYDPNEP SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD ccccccccccccccccccccceeecccccccccccccccccccccccccceeeecccccc COILS

SEQ RYCICNQVKVCYIYKSII SEG PRD eeeeceeeeeeeeeeccc COILS

Prosite for DKFZphutel_18cl2.1

PS00001 190->194 ASN_GLYCOSYLATION PDOC00001

PS00001 191->195 ASN_GLYCOSYLATION PDOC00001

PS00001 203->207 ASN_GLYCOSYLATION PDOC00001

PS00001 288->292 ASN_GLYCOSYLATION PDOC00001

PΞ00001 306->310 ASN_GLYCOSYLATION PDOC00001

PS00002 218->222 GLYCOSAMINOGLYCAN PDOC00002

PS00004 243->247 CAMP_PHOSPHO_SITE PDOC00004

PS00005 64->67 PKC_PHOSPHO_SITE PDOC00005

PS00005 247->250 PKC_PHOSPHO_SITE PDOC00005

PS00005 298->301 PKC_PHOSPHO_SITE PDOC00005

PΞ00006 142->146 CK2_PHOSPHO_SITE PDOC00006

PS00006 156->160 CK2_PHOSPHO_SITE PDOC00006

PS00006 292->296 CK2_PHOΞPHO_SITE PDOC00006

PS00006 349->353 CK2_PHOSPHO_SITE PDOC00006

PS00008 186->192 MYRISTYL PDOC00008

PS00008 214->220 MYRISTYL PDOC00008

PS00008 219->225 MYRISTYL PDOC00008

PS00009 241->245 AMIDATION PDOC00009

PS00009 298->302 AMIDATION PDOC00009

PS00013 315->326 PROKAR LIPOPROTEIN PDOC00013

(No Pfam data available for DKFZphutel 18cl2.1) DKFZphutel_18ιl9

group: transcription factors

DKFZphutel_18ιl9 encodes a novel 759 ammo acid protein with similarity to the SREBP-2 mutant sterol regulatory element binding protem-2 of Cricetulus gπseus.

The SREBP-2 protein is embedded in the membranes of the nucleus and endoplasmic reticulum. In cholesterol-depleted cells the proteins are cleaved to release soluble NH2-termmal fragments that enter the nucleus and activate genes encoding the low density lipoprotein receptor and enzymes of cholesterol synthesis. The new protein is a putative transcription factor capable of protein-protein interaction via a lim domain and additionally shows similarity to the common sunflower transcription factor SF3.

The new protein can find application in modulating/blocking the expression of genes involved m lipid metabolism. similarity to transcription factor ΞF3 complete cDNA, complete eds, EST hits strong similarity to mutated SREBP-2 of hamster, similarity is not to SREP-2 part of protein but to the unknown part of the fusion protein

Sequenced by AGOWA

Locus: /map=12

Insert length: 3664 bp

Poly A stretch at pos. 3647, polyadenylation signal at pos. 3636

1 GCGCTAGGTA GAGCGCCGGG ACCTGTGACA GGGCTGGTAG CAGCGCAGAG

51 GAAAGGCGGC TTTTAGCCAG GTATTTCAGT GTCTGTAGAC AAGATGGAAT

101 CATCTCCATT TAATAGACGG CAATGGACCT CACTATCATT GAGGGTAACA

151 GCCAAAGAAC TTTCTCTTGT CAACAAGAAC AAGTCATCGG CTATTGTGGA

201 AATATTCTCC AAGTACCAGA AAGCAGCTGA AGAAACAAAC ATGGAGAAGA

251 AGAGAAGTAA CACCGAAAAT CTCTCCCAGC ACTTTAGAAA GGGGACCCTG

301 ACTGTGTTAA AGAAGAAGTG GGAGAACCCA GGGCTGGGAG CAGAGTCTCA

351 CACAGACTCT CTACGGAACA GCAGCACTGA GATTAGGCAC AGAGCAGACC

401 ATCCTCCTGC TGAAGTGACA AGCCACGCTG CTTCTGGAGC CAAAGCTGAC

451 CAAGAAGAAC AAATCCACCC CAGATCTAGA CTCAGGTCAC CTCCTGAAGC

501 CCTCGTTCAG GGTCGATATC CCCACATCAA GGACGGTGAG GATCTTAAAG

551 ACCACTCAAC AGAAAGTAAA AAAATGGAAA ATTGTCTAGG AGAATCCAGG

601 CATGAAGTAG AAAAATCAGA AATCAGTGAA AACACAGATG CTTCGGGCAA

651 AATAGAGAAA TATAATGTTC CGCTGAACAG GCTTAAGATG ATGTTTGAGA

701 AAGGTGAACC AACTCAAACT AAGATTCTCC GGGCCCAAAG CCGAAGTGCA

751 AGTGGAAGGA AGATCTCTGA AAACAGCTAT TCTCTAGATG ACCTGGAAAT

801 AGGCCCAGGT CAGTTGTCAT CTTCTACATT TGACTCGGAG AAAAATGAGA

851 GTAGACGAAA TCTGGAACTT CCACGCCTCT CAGAAACCTC TATAAAGGAT

901 CGAATGGCCA AGTACCAGGC AGCTGTGTCC AAACAAAGCA GCTCAACCAA

951 CTATACAAAT GAGCTGAAAG CCAGTGGTGG CGAAATCAAA ATTCATAAAA

1001 TGGAGCAAAA GGAGAATGTG CCCCCAGGTC CTGAGGTCTG CATCACCCAT

1051 CAGGAAGGGG AAAAGATTTC TGCAAATGAG AATAGCCTGG CAGTCCGTTC

1101 CACCCCTGCC GAAGATGACT CCCGTGACTC CCAGGTTAAG AGTGAGGTTC

1151 AACAGCCTGT CCATCCCAAG CCACTAAGTC CAGATTCCAG AGCCTCCAGT

1201 CTTTCTGAAA GTTCTCCTCC CAAAGCAATG AAGAAGTTTC AGGCACCTGC

1251 AAGAGAGACC TGCGTGGAAT GTCAGAAGAC AGTCTATCCA ATGGAGCGTC

1301 TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGCTCCTAT

1351 TGCAACAACA AACTCAGTCT AGGAACATAT GCATCTTTAC ATGGAAGAAT

1401 CTATTGTAAG CCTCACTTCA ATCAACTCTT TAAATCTAAG GGCAACTATG

1451 ATGAAGGCTT TGGGCACAGA CCACACAAGG ATCTATGGGC AAGCAAAAAT

1501 GAAAACGAAG AGATTTTGGA GAGACCAGCC CAGCTTGCAA ATGCAAGGGA

1551 GACCCCTCAC AGCCCAGGGG TAGAAGATGC CCCTATTGCT AAGGTGGGTG

1601 TCCTGGCTGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCA GGAGAAGGAA

1651 GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC

1701 TGAACTTGGA AGTTCAGGAA GTGCCTTGGA GGAAGGGATC AAAATGTCAA

1751 AGCCCAAATG GCCTCCTGAA GACGAAATCA GCAAGCCCGA AGTTCCTGAG

1801 GATGTCGATC TAGATCTGAA GAAGCTAAGA CGATCTTCTT CACTGAAGGA

1851 AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCAAAGC ACCTCTGTCA

1901 AGAGCCCAAA AACTGTGTCC CCACCTATCA GGAAAGGCTG GAGCATGTCA

1951 GAGCAGAGTG AAGAGTCTGT GGGTGGAAGA GTTGCAGAAA GGAAACAAGT

2001 GGAAAATGCC AAGGCTTCTA AGAAGAATGG GAATGTGGGA AAAACAACCT

2051 GGCAAAACAA AGAATCTAAA GGAGAGACAG GGAAGAGAAG TAAGGAAGGT

2101 CATAGTTTGG AGATGGAGAA TGAGAATCTT GTAGAAAATG GTGCAGACTC

2151 CGATGAAGAT GATAACAGCT TCCTCAAACA ACAATCTCCA CAAGAACCCA

2201 AGTCTCTGAA TTGGTCGAGT TTTGTAGACA ACACCTTTGC TGAAGAATTC

2251 ACTACTCAGA ATCAGAAATC CCAGGATGTG GAACTCTGGG AGGGAGAAGT 2301 GGTCAAAGAG CTCTCTGTGG AAGAACAGAT AAAGAGAAAT CGGTATTATG

2351 ATGAGGATGA GGATGAAGAG TGACAAATTG CAATGATGCT GGGCCTTAAA

2401 TTCATGTTAG TGTTAGCGAG CCACTGCCCT TTGTCAAAAT GTGATGCACA

2451 TAAGCAGGTA TCCCAGCATG AAATGTAATT TACTTGGAAG TAACTTTGGA

2501 AAAGAATTCC TTCTTAAAAT CAAAAACAAA ACAAAAAAAC ACAAAAAACA

2551 CATTCTAAAT ACTAGAGATA ACTTTACTTA AATTCTTCAT TTTAGCAGTG

2601 ATGATATGCG TAAGTGCTGT AAGGCTTGTA ACTGGGGAAA TATTCCACCT

2651 GATAATAGCC CAGATTCTAC TGTATTCCCA AAAGGCAATA TTAAGGTAGA

2701 TAGATGATTA GTAGTATATT GTTACACACT ATTTTGGAAT TAGAGAACAT

2751 ACAGAAGGAA TTTAGGGGCT TAAACATTAC GACTGAATGC ACTTTAGTAT

2801 AAAGGGCACA GTTTGTATAT TTTTAAATGA ATACCAATTT AATTTTTTAG

2851 TATTTACCTG TTAAGAGATT ATTTAGTCTT TAAATTTTTT AGGTTAATTT

2901 TCTTGCTGTG ATATATATGA GGAATTTACT ACTTTATGTC CTGCTCTCTA

2951 AACTACATCC TGAACTCGAC GTCCTGAGGT ATAATACAAC AGAGCACTTT

3001 TTGAGGCAAT TGAAAAACCA ACCTACACTC TTCGGTGCTT AGAGAGATCT

3051 GCTGTCTCCC AAATAAGCTT TTGTATCTGC CAGTGAATTT ACTGTACTCC

3101 AAATGATTGC TTTCTTTTCT GGTGATATCT GTGCTTCTCA TAATTACTGA

3151 AAGCTGCAAT ATTTTAGTAA TACCTTCGGG ATCACTGTCC CCCATCTTCC

3201 GTGTTAGAGC AAAGTGAAGA GTTTAAAGGA GGAAGAAGAA AGAACTGTCT

3251 TACACCACTT GAGCTCAGAC CTCTAAACCC TGTATTTCCC TTATGATGTC

3301 CCCTTTTTGA GACACTAATT TTTAAATACT TACTAGCTCT GAAATATATT

3351 GATTTTTATC ACAGTATTCT CAGGGTGAAA TTAAACCAAC TATAGGCCTT

3401 TTTCTTGGGA TGATTTTCTA GTCTTAAGGT TTGGGGACAT TATAAACTTG

3451 AGTACATTTG TTGTACACAG TTGATATTCC AAATTGTATG GATGGGAGGG

3501 AGAGGTGTCT TAAGCTGTAG GCTTTTCTTT GTACTGCATT TATAGAGATT

3551 TAGCTTTAAT ATTTTTTAGA GATGTAAAAC ATTCTGCTTT CTTAGTCTTA

3601 CCTAGTCTGA AACATTTTTA TTCAATAAAG ATTTTAATTA AAATTTGAAA

3651 AAAAAAAAAA AAAA

BLAST Results

Entry HS512217 from database EMBL: human STS SHGC-14654.

Length = 250

Minus Strand HSPs:

Score = 1202 (180.3 bits), Expect = 1.8e-46, P = 1.8e-46

Identities = 242/244 (99%)

Medline entries

95263566:

Three different rearrangements in a single intron truncate sterol regulatory element binding proteιn-2 and produce sterol-resistant phenotype in three cell lines. Role of introns in protein evolution.

93258417:

Characterization of a pollen-specific cDNA from sunflower encoding a zinc finger protein.

Peptide information for frame 1

ORF from 94 bp to 2370 bp; peptide length: 759 Category: similarity to known protein

1 MESSPFNRRQ WTSLSLRVTA KELSLVNKNK SSAIVEIFSK YQKAAEETNM 51 EKKRSNTENL SQHFRKGTLT VLKKKWENPG LGAESHTDSL RNSSTEIRHR 101 ADHPPAEVTS HAASGAKADQ EEQIHPRSRL RSPPEALVQG RYPHIKDGED 151 LKDHSTESKK MENCLGESRH EVEKSEISEN TDASGKIEKY NVPLNRLKMM 201 FEKGEPTQTK ILRAQSRSAS GRKISENSYS LDDLEIGPGQ LSSSTFDSEK 251 NESRRNLELP RLSETSIKDR MAKYQAAVSK QSSSTNYTNE LKASGGEIKI 301 HKMEQKENVP PGPEVCITHQ EGEKISANEN SLAVRSTPAE DDSRDSQVKS 351 EVQQPVHPKP LSPDSRASSL ΞESSPPKAMK KFQAPARETC VECQKTVYPM 401 ERLLANQQVF HISCFRCSYC NNKLSLGTYA SLHGRIYCKP HFNQLFKSKG 451 NYDEGFGHRP HKDLWASKNE NEEILERPAQ LANARETPHS PGVEDAPIAK 501 VGVLAASMEA KASSQQEKED KPAETKKLRI AWPPPTELGS SGSALEEGIK 551 MSKPKWPPED EISKPEVPED VDLDLKKLRR SSSLKERSRP FTVAASFQST 601 SVKSPKTVSP PIRKGWSMSE QSEESVGGRV AERKQVENAK ASKKNGNVGK 651 TTWQNKESKG ETGKRSKEGH SLEMENENLV ENGADSDEDD NSFLKQQSPQ 701 EPKSLNWSSF VDNTFAEEFT TQNQKSQDVE LWEGEVVKEL SVEEQIKRNR 751 YYDEDEDEE

BLASTP hits

Entry CG22818_1 from database TREMBL:

"SREBP-2"; product: "mutant sterol regulatory element binding protem-2"; Cricetulus griseus SRD-2 mutant sterol regulatory element binding proteιn-2 (SREBP-2) mRNA, complete eds. Cricetulus griseus (Chinese hamster)

Length = 839

Score = 1502 (528.7 bits), Expect = 3.9e-154, P = 3.9e-154

Identities = 290/380 (76%), Positives = 322/380 (84%)

Entry S28507 from database PIR: transcription factor SF3 - common sunflower

Length = 219

Score = 212 (74.6 bits), Expect = 6.3e-18, Sum P(2) = 6.3e-18

Identities = 36/82 (43%), Positives = 55/82 (67%)

Entry NTLIMDOM_l from database TREMBL:

"SF3"; product: "LIM-domain SF3 protein"; N.tabacum mRNA for

LIM-domain protein Nicotiana tabacum (common tobacco)

Length = 189

Score = 216 (76.0 bits), Expect = 1.0e-16, P = 1.0e-16

Identities = 42/94 (44%), Positives = 57/94 (60%)

Alert BLASTP hits for DKFZphutel_18ιl9, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphutel_18ιl9, frame 1

Report for DKFZphutel_18ιl9.1

[LENGTH] 759

[MW] 85225.57

[pi] 6.41

[HOMOL] TREMBL :CG22818_1 gene: "SREBP-2"; product: "mutant sterol regulatory element binding prote n-2"; Cricetulus griseus SRD-2 mutant sterol regulatory element binding protein-

2 (SREBP-2) mRNA, complete eds. le-151

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YLR257w] 3e-05

[FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae,

YGR162W TIF4631 - mRNA cap-binding protein] le-04

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YGR162w TIF4631 mRNA cap-bmding protein] le-04

[BLOCKS] BL00478B

[PIRKW] zinc finger 9e-16

[PIRKW] DNA binding 9e-16

[SUPFAM] LIM metal-binding repeat homology 9e-16

[PROSITE] MYRISTYL 6

[PROSITE] LIM_DOMAIN_l 1

[PROSITE] AMIDATION 2

[PROSITE] CAMP_PHOSPHO_SITE 4

[PROSITE] CK2_PHOSPHO_SITE 28

[PROSITE] TYR_PHOSPHO_SITE 2

[PROSITE] PKC_PHOSPHO_SITE 15

[PROSITE] ASN_GLYCOSYLATION 6

[PFAM] LIM domain containing proteins

[KW] Irregular

[KW] 3D

[KW] LOW COMPLEXITY 5.53 %

SEQ MESSPFNRRQWTSLΞLRVTAKELSLVNKNKSSAIVEIFSKYQKAAEETNMEKKRSNTENL

SEG lctl-

SEQ SQHFRKGTLTVLKKKWENPGLGAESHTDSLRNSSTEIRHRADHPPAEVTSHAASGAKADQ

SEG lctl-

SEQ EEQIHPRSRLRSPPEALVQGRYPHIKDGEDLKDHSTESKKMENCLGESRHEVEKSEISEN

SEG lctl-

SEQ TDASGKIEKYNVPLNRLKMMFEKGEPTQTKILRAQSRΞASGRKISENSYSLDDLEIGPGQ

SEG lctl-

SEQ LSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKI SEG lctl-

SEQ HKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDSQVKSEVQQPVHPKP SEG x lctl-

SEQ LSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYC SEG xxxxxxxxxxxxxxxx lctl- ETTTTEEETTTCEEEETTEEEETTTTBTTTT

SEQ NNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQ SEG lctl- TCBCBTTBEEEETTEEEETTTTTTTTTTCCTTTTTTTCTTT

SEQ LANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGS SEG lctl-

SEQ SGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQST SEG xxxxxxxxxxxxxxxxxx lctl-

SEQ SVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKG SEG lctl-

SEQ ETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFT SEG lctl-

SEQ TQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE SEG xxxxxxx lctl-

Prosite for DKFZphutel_18ιl9.1

PS00001 29->33 ASN_GLYCOΞYLATION PDOC00001 PS00001 59->63 ASN_GLYCOSYLATION PDOC00001 PΞ00001 92->96 ASN_GLYCOSYLATION PDOC00001 PS00001 251->255 ASN_GLYCOSYLATION PDOC00001 PS00001 286->290 ASN_GLYCOSYLATION PDOC00001 PS00001 706->710 ASN_GLYCOSYLATION PDOC00001 PS00004 52->56 CAMP_PHOSPHO_SITE PDOC00004 PS00004 65->69 CAMP_PHOSPHO_SITE PDOC00004 PS00004 222->226 CAMP_PHOSPHO_SITE PDOC00004 PS00004 579->583 CAMP_PHOSPHO_SITE PDOC00004 PS00005 15->18 PKC_PHOSPHO_SITE PDOC00005 PS00005 19->22 PKC_PHOSPHO_SITE PDOC00005 PS00005 89->92 PKC_PHOSPHO_SITE PDOC00005 PS00005 158->161 PKC_PHOSPHO_SITE PDOC00005 PS00005 184->187 PKC_PHOSPHO_SITE PDOC00005 PS00005 220->223 PKC_PHOSPHO_SITE PDOC00005 PS00005 248->251 PKC_PHOSPHO_SITE PDOC00005 PS00005 253->256 PKC_PHOSPHO_SITE PDOC00005 PS00005 266->269 PKC_PHOSPHO_SITE PDOC00005 PS00005 525->528 PKC_PHOSPHO_SITE PDOC00005 PS00005 583->586 PKC_PHOSPHO_SITE PDOC00005 PS00005 601->604 PKC_PHOSPHO_SITE PDOC00005 PS00005 604->607 PKC_PHOSPHO_SITE PDOC00005 PS00005 642->645 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 662->665 PKC_PHOSPHO_SITE PDOC00005 PS00006 19->23 CK2_PHOSPHO_SITE PDOC00006 PS00006 48->52 CK2_PHOSPHO_SITE PDOC00006 PS00006 55->59 CK2_PHOSPHO_SITE PDOC00006 PS00006 85->89 CK2_PHOSPHO_SITE PDOC00006 PS00006 93->97 CK2_PHOSPHO_SITE PDOC00006 PS00006 132->136 CK2_PHOSPHO_SITE PDOC00006 PS00006 168-M72 CK2_PHOSPHO_SITE PDOC00006 PS00006 230->234 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 244->248 CK2_PHOSPHO_SITE PDOC00006 PS00006 ₂66->270 CK2_PHOΞPHO_SITE PDOC00006 PS00006 294->298 CK2_PHOSPHO_SITE PDOC00006 PS00006 318->322 CK2_PHOSPHO_SITE PDOC00006 PS00006 326->330 CK2_PHOSPHO_SITE PDOC00006 PS00006 337->341 CK2 PHOSPHO SITE PDOC00006 PS00006 369-->373 CK2 PHOSPHO SITE PDOC00006

PS00006 389- ->393 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 467- ->471 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 514- ->518 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 543- ->547 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 563- ->567 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 583- ->587 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 617- ->621 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 658- ->662 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 686- ->690 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 698- ->702 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 709- ->713 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 714- ->718 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 741- ->745 CK2 PHOSPHO^{" "}SITE PDOC00006

PΞ00007 223- ->230 TYR PHOSPHO^{" "}SITE PDOC00007

PS00007 222- ->230 TYR PHOSPHO^{" "}SITE PDOC00007

PS00008 2₃9- ->245 MYRISTYL PDOC00008

PS00008 427- ->433 MYRISTYL PDOC00008

PS00008 502- ->508 MYRISTYL PDOC00008

PS00008 539- ->545 MYRISTYL PDOC00008

PS00008 548- ->554 MYRISTYL PDOC00008

PS00008 627- ->633 MYRISTYL PDOC00008

PS00009 220- ->224 AMIDATION PDOC00009

PS00009 662- ->666 AMIDATION PDOC00009

PS00478 390- ->425 LIM DOMAIN ] PDOC00382

Pfam for DKFZphutel_18ιl9.1

HMM_NAME LIM domain containing proteins

HMM *CagCNrpIyDREιvMRAMNKvWHpECFrCcdCqqPLtegdeFYErDGrI

C C++++Y+ E++ A+ V+H++CFRC+ C+ L+ G+ + ++ GRI Query 390 CVECQKTVYPMERLL-ANQQVFHISCFRCSYCNNKLSLGT-YASLHGRI 436

HMM YCKhDYYrrFg*

YCK+++ ++F+ Query 437 YCKPHFNQLFK 447

DKFZphutel_18ι4

group: uterus derived

DKFZphutel_18ι4 encodes a novel 220 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of uterus-specific genes . weak similarity to C. elegans D2085.2 complete cDNA, complete eds, few EST hits

Sequenced by AGOWA

Locus: /map="7q31"

Insert length: 1568 bp

Poly A stretch at pos. 1551, polyadenylation signal at pos. 1523

1 GCCGAGCGGA GAGGGTAGAG ACGGGGTTTC ACCGTGTTAG CCAAGATGGT 51 CTCGATCTCC TGACCTCGTG ATCCGCCCGC CTCGGCCTCC CAAAGTGCTG

101 GGATTACAGG CGTGAGCCAC TGCGCCCGGC CTGTTGTACA GTTATTAAAG

151 TTATCATTTA ACATGGAAGA AGATGAGTTC ATTGGAGAAA AAACATTCCA

201 ACGTTATTGT GCAGAATTCA TTAAACATTC ACAACAGATA GGTGATAGTT

251 GGGAATGGAG ACCATCAAAG GACTGTTCTG ATGGCTACAT GTGCAAAATA

301 CACTTTCAAA TTAAGAATGG GTCTGTGATG TCACATCTAG GAGCATCTAC

351 CCATGGACAG ACATGTCTTC CCATGGAGGA GGCTTTCGAG CTACCCTTGG

401 ATGATTGTGA AGTGATTGAA ACTGCAGCAG CGTCCGAAGT GATTAAATAT

451 GAGTATCATG TCTTATATTC CTGTAGCTAC CAAGTGCCTG TACTTTACTT

501 TAGGGCAAGC TTTTTAGATG GGAGACCTTT AACTCTGAAG GACATATGGG

551 AAGGAGTTCA TGAGTGCTAT AAGATGCGAC TGCTACAGGG ACCATGGGAC

601 ACTATTACGC AACAGGAACA TCCAATACTT GGGCAACCCT TTTTTGTACT

651 TCATCCCTGC AAGACGAATG AATTCATGAC TCCTGTATTA AAGAATTCTC

701 AGAAAATCAA TAAGAATGTC AACTATATCA CATCATGGCT GAGCATTGTA

751 GGGCCAGTTG TTGGGCTGAA TCTACCTCTG AGTTATGCCA AAGCAACGTC

801 TCAGGATGAA CGAAATGTCC CTTAACAAGA TTCTTCTATT GAGTTTAGGA

851 ATTGCGGCAC GAAGAATGCC AAGAGTTTAC CTGGCCAGCC CTGGCTTTAA

901 TAGGACTGAT ACCATGGAAT ATTTCATCTC ACCAAGATGT GACATGGATT

951 ATTTTTCCCT TGGACACAAA TGTCTACAGC AACTGATGTT TGATAGGCTG 1001 AATGTTTAGA AGAAACACTT CAAAGGGATA CATCATGGCC AGGCATGGTG 1051 GCTCACACCT GTAATCCAAG CACTTTGGGA GGCCAAGGTG GGAGCATCAC 1101 TTGATCCTGG GAGTTCGAGA CCAGCCTGGG CAACATGGTG AAACCCTGTC 1151 GGTACAAAAA AATACAAAAA TTTGCCTGTT TATGGTGGTG TGTTCCTGTA 1201 GTCCCAGCTC CCCAGGAGGC TGAGGTGGGA GGTTGGCTTT AACCCAGGAG 1251 GCAGAGGTTG CAGTGAGCTG AGACTGTGCC ACTGCAGTCC AGCCTGGGTG 1301 ACAGAGCCAG ACACTGTCTC GGGAAAAAAA AAAAAAAAAA AAAGACACAT 1351 CACTATAAAT AGCAAAAAAA CAAATCTAAC TTATTAA AC TAGGAATACC 1401 AACATTATTA GGGCACTTGC AGGTTATTCT TTTCTAGGCC AAGTACTTCA 1451 CTTCCATTTG TCTGACATGG AGATTGAGGG AGAAATGTAT TTGTGTGTTC 1501 ATTTTAATGT AAGATATATA AAAATTAAAT TACTGGATTT ACCTGTCCCT 1551 GAAAAAAAAA AAAAAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 1

ORF from 163 bp to 822 bp; peptide length: 220 Category: similarity to unknown protein 1 MEEDEFIGEK TFQRYCAEFI KHSQQIGDSW EWRPSKDCSD GYMCKIHFQI

51 KNGSVMSHLG ASTHGQTCLP MEEAFELPLD DCEVIETAAA SEVIKYEYHV

101 LYSCSYQVPV LYFRASFLDG RPLTLKDIWE GVHECYKMRL LQGPWDTITQ

151 QEHPILGQPF FVLHPCKTNE FMTPVLKNSQ KINKNVNYIT SWLSIVGPVV

201 GLNLPLSYAK ATSQDERNVP

BLASTP hits

Entry CED2085_2 from database TREMBL:

"D2085.2"; Caenorhabditis elegans cosmid D2085

Length = 173

Score = 167 (58.8 bits), Expect = 1. le-12, P = 1. le-12

Identities = 36/121 (29%), Positives = 64/121 (52%)

Alert BLASTP hits for DKFZphutel_18ι4, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphutel_18ι4, frame 1

Report for DKFZphutel_18ι4.1

[LENGTH] 220 [MW] 25278.99 [pi] 5.34 [HOMOL] TREMBL :CED2085_2 gene: "D2085.2"; Caenorhabditis elegans cosmid D2085 2e-ll

[BLOCKS] BL00221E

[PROSITE] MYRISTYL 2

[PROSITE] CK2_PHOSPHO_SITE

[PROSITE] PKC_PHOSPHO_SITE

[PROSITE] ASN_GLYCOSYLATION

[KW] Alpha_Beta

SEQ MEEDEFIGEKTFQRYCAEFIKHSQQIGDSWEWRPSKDCSDGYMCKIHFQIKNGΞVMSHLG PRD cccccccchhhhhhhhhhhhhhhhcccccccccccccccceeeeeeeeeeeccceeeeec

SEQ ASTHGQTCLPMEEAFELPLDDCEVIETAAAΞEVIKYEYHVLYSCSYQVPVLYFRASFLDG PRD cccccccchhhhhhhhccccceeehhhhhchhhhhhhheeeeccccceeeeeeecccccc

SEQ RPLTLKDIWEGVHECYKMRLLQGPWDTITQQEHPILGQPFFVLHPCKTNEFMTPVLKNΞQ PRD cccccchhhhhhhhhhhhhhhhccccccccccccccccceeeeccccccccccccccccc

SEQ KINKNVNYITSWLSIVGPVVGLNLPLSYAKATSQDERNVP

PRD ccccccccccccceeeeccccccccceeeecccccccccc

Prosite for DKFZphutel_18ι4.1

PS00001 52->56 ASN_GLYCOSYLATION PDOC00001 PS00005 124->127 PKC_PHOSPHO_SITE PDOC00005 PS00005 179->182 PKC_PHOSPHO_SITE PDOC00005 PS00006 116-M20 CK2_PHOSPHO_SITE PDOC00006 PS00006 124->128 CK2_PHOSPHO_SITE PDOC00006 PS00006 149->153 CK2_PHOSPHO_SITE PDOC00006 PS00006 212->216 CK2_PHOSPHO_SITE PDOC00006 PS00008 53->59 MYRISTYL PDOC00008 PS00008 131->137 MYRISTYL PDOC00008

(No Pfam data available for DKFZphutel_18ι4.1) DKFZphutel 1811

group: nucleic acid management

DKFZphtes3_15jl8 encodes a novel 184 amino acid protein with similarity to S. cerevisiae putative ribosomal protein YHR148w.

The novel protein is similar to several 40S ribosomal proteins and therefore seems to part of the corresponding ribosome subunit.

The new protein can find application in modulation of ribosome assembly, structure and function. strong similarity to S. cerevisiae YHR148w complete cDNA, complete eds, EST hits, potential start at Bp 45 matchs kozak consensus ANNatgG gene disruption of YHR148w is lethal¹

Sequenced by AGOWA

Locus : unknown

Insert length: 1076 bp

Poly A stretch at pos. 1035, polyadenylation signal at pos. 1006

1 GCGCGCTCTC AGCTTCGGGT CCTGCGGCTG CGGCTGCCGC CATCATGGTG

51 CGGAAGCTTA AGTTCCACGA GCAGAAGCTG CTGAAGCAGG TGGACTTCCT

101 GAACTGGGAG GTCACCGACC ACAACCTGCA CGAGCTGCGC GTGCTGCGGC

151 GTTACCGGCT GCAGCGGCGG GAGGACTACA CGCGCTACAA CCAGCTGAGC

201 CGTGCCGTGC GTGAGCTGGC GCGGCGCCTG CGCGACCTGC CCGAACGCGA

251 CCAGTTCCGC GTGCGCGCTT CGGCCGCGCT GCTGGACAAG CTGTATGCTC

301 TCGGCTTGGT GCCCACGCGC GGTTCGCTGG AGCTCTGCGA CTTCGTCACG

351 GCCTCGTCCT TCTGCCGCCG CCGCCTCCCC ACCGTGCTCC TCAAGCTGCG

401 CATGGCGCAG CACCTTCAGG CTGCCGTGGC CTTTGTGGAG CAAGGGCACG

451 TACGCGTGGG CCCTGACGTG GTTACCGACC CCGCCTTCCT TGTCACGCGC

501 AGCATGGAGG ACTTTGTCAC TTGGGTGGAC TCGTCCAAGA TCAAGCGGCA

551 CGTGCTAGAG TACAATGAGG AGCGCGATGA CTTCGATCTG GAAGCCTAGC

601 GGATCTCCCA CTTTGCATGG CTGTCTTTTA CAGATGGGAA AACTGAGGCC

651 TGATGCTGGA GATTCTATGA GGGTGCTCTC CTCAAGGGTA TCAGACGGTC

701 GTAGGTTCTT AAGAATTTGA TTCATCAGTG GCAGGCCATG CATAGAGCCA

751 CGGGAGGTGC GTCCTTGTTT TCCAGGAAAT GTTCTTAGAA CTTGGACTAC

801 TGATTATTAA TTGACTGTGC CTTGGGAAAC AGTGGGAAGT AACTTGGTGC

851 AGCACTGGGG TATTGTTGGA CTGGTTCAAT TCGTTTAACT CGAATTCTTG

901 CTCCTGGCCG TGGTTAAGCT GTGTACAGAT GATGGAGAGT TTGGCCTCAA

951 GTTTTTATAA ACTGAGCGAG ACTAGTGTTC AGGATCTCCT CCCTTGTTTA

1001 AATGTCAATA AATGCCCCAA CTGCTTTGTA AGCTCAAAAA AAAAAAAAAA

1051 AAAAAAAAAA AAAAAAAAAA AAAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 45 bp to 596 bp; peptide length: 184 Category: strong similarity to known protein

1 MVRKLKFHEQ KLLKQVDFLN WEVTDHNLHE LRVLRRYRLQ RREDYTRYNQ

51 LSRAVRELAR RLRDLPERDQ FRVRASAALL DKLYALGLVP TRGSLELCDF

101 VTASSFCRRR LPTVLLKLRM AQHLQAAVAF VEQGHVRVGP DVVTDPAFLV

151 TRSMEDFVTW VDSSKIKRHV LEYNEERDDF DLEA

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphutel_1811, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphutel_1811, frame 3

Report for DKFZphutel_1811.3

[LENGTH] 184

[MW] 21850.21

[pi] 9.54

[HOMOL] PIR:S33911 probable ribosomal protein YHR148w - yeast (Saccharomyces cerevisiae) 4e-•47

[ FUNCAT] 05.01 ribosomal proteins [S. cerevisiae, YHR148w] 2e-48

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YPL081w] 5e-07

[FUNCAT] j mrna translation and ribosome biogenesis [M. jannaschn, MJ0190] 8e-05

[BLOCKS] BL00632

[PIRKW] cytosol le-07

[PIRKW] ribosome le-07

[PIRKW] protein biosynthesis le-07

[SUPFAM] rat ribosomal protein S9 le-07

[PROSITE] MYRISTYL 1

[PROSITE] CK2_PHOSPHO_SITE 2

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 1

[PFAM] Ribosomal protein S4

[KW] All_Alpha

[KW] LOW COMPLEXITY 6.52 %

SEQ MVRKLKFHEQKLLKQVDFLNWEVTDHNLHELRVLRRYRLQRREDYTRYNQLSRAVRELAR SEG xxxxxxxxxxxx PRD ccchhhhhhhhhhhhhhhhhcccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ RLRDLPERDQFRVRASAALLDKLYALGLVPTRGSLELCDFVTASSFCRRRLPTVLLKLRM SEG PRD hhhhhccccchhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ AQHLQAAVAFVEQGHVRVGPDVVTDPAFLVTRSMEDFVTWVDSSKIKRHVLEYNEERDDF SEG PRD hhhhhhhhhhhhhhhccccceeecccceeeeeccccceeeeeccchhhhhhhhhcccccc

SEQ DLEA SEG PRD

Prosite for DKFZphutel_1811.3

PS00005 163->166 PKC_PHOSPHO_ΞITE PDOC00005

PS00006 153->157 CK2_PHOSPHO_SITE PDOC00006

PS00006 159->163 CK2_PHOSPHO_SITE PDOC00006

PS00007 41->49 TYR_PHOSPHO_SITE PDOC00007

PS00008 87->93 MYRISTYL PDOC00008

Pfam for DKFZphutel_1811.3

HMM_NAME Ribosomal protein S4

HMM *MSR.YRGPRWKIIRRPGElPWLTnK tklmrkYC..lRPgQHgWR

M+R ++ +++K+++++++L W ++++R Y R+++ ++

Query 1 MVRKLKFHEQKLLKQVDFLNWEVTDHNLHELRVLRRYRLQRREDYTRYN 49

HMM qRktLsKIRRmSQYrlRLQEKQKLRFMYGNItERQLRRYvRiaEdKRKID

Q + +R +++ + L+E + +R +++++L++++ +++ L Query 50 QLSR—AVRELARRLRDLPERDQFRVRASAALLDKLYALGLVP-TRGSLE 96

HMM YsTGenLMQILEMRLDNIVFRMGMAPTIHHARQLINHRHIRVNdRIVNIP

++ + ++++RL++++ ++ MA ++A+ +++++H+RV++ +V++P Query 97 LCDFVTASSFCRRRLPTVLLKLRMAQHLQAAVAFVEQGHVRVGPDVVTDP 146

HMM SYiCRPNDiISIRDkqrMQsHIkWmeSPegrmRPNHLErNnkkYeGtlN ++++++ + +++++W++ S+ ++R+ + Y+ +

Query 147 AFLVTRS M EDFVTWVDSSK IKRHVLEYNEERD 178

HMM rllEReWiplklNElLVVEY*

+++ + Query 179 DFDLE 183

DKFZphutel_19fl9

group: transmembrane protein

DKFZphutel_19f19 encodes a novel 204 ammo acid protein with similarity to murine p24 protein.

Murine p24 is expressed only in brain where it is localized exclusively in neurons. It seems to be a neuron-specific membrane protein localised in intracellular organelles of highly differentiated neural cells and may play a role in the neural organelle transport system. As p24, the novel protein contains 2 transmembrane regions, but it contains not the sequence homologous to the microtubule-binding domain of microtubule-associated proteins present in p24.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application m studying the expression profile of uterus-specific genes and as a new marker for uterine cells. similarity to mouse P24 protein ; membrane regions : 2

Summary DKFZphutel_19f19 encodes a novel 204 amino acid protein, with similarity to mouse P24 protein. similarity to mouse P24 protein complete cDNA, complete eds, EST hits, 2 TM-domams

Sequenced by AGOWA

Locus: /map=14.8 cR from top of Chr20 linkage group

Insert length: 2042 bp

Poly A stretch at pos. 1958, polyadenylation signal at pos. 1940

1 GCAGGCAGAG AGATGAGGAA ACTGAGACCC AGAAAGGTGG AAGCACTTGT

51 CTAAGGTCAC GCCTCCAGGA AGCAGTGTGT CCACGACTCC AGTCCAAGTG

101 GTCAGGCTCC AGAGCCCACA GTCCCAGGGG TCCATGATGC CGAGCTGCAA

151 TCGTTCCTGC AGCTGCAGCC GCGGCCCCAG CGTGGAGGAT GGCAAGTGGT

201 ATGGGGTCCG CTCCTACCTG CACCTCTTCT ATGAGGACTG TGCAGGCACT

251 GCTCTCAGCG ACGACCCTGA GGGACCTCCG GTCCTGTGCC CCCGCCGGCC

301 CTGGCCCTCA CTGTGTTGGA AGATCAGCCT GTCCTCGGGG ACCCTGCTTC

351 TGCTGCTGGG TGTGGCGGCT CTGACCACTG GCTATGCAGT GCCCCCCAAG

401 CTGGAGGGCA TCGGTGAGGG TGAGTTCCTG GTGTTGGATC AGCGGGCAGC

451 CGACTACAAC CAGGCCCTGG GCACCTGTCG CCTGGCAGGC ACAGCGCTCT

501 GTGTGGCAGC TGGAGTTCTG CTCGCCATCT GCCTCTTCTG GGCCATGATA

551 GGCTGGCTGA GCCAGGACAC CAAGGCAGAG CCCTTGGACC CCGAAGCCGA

601 CAGCCACGTG GAGGTCTTCG GGGATGAGCC AGAGCAGCAG TTGTCACCCA

651 TTTTCCGCAA TGCCAGTGGC CAGTCATGGT TCTCGCCACC CGCCAGCCCC

701 TTTGGGCAAT CTTCTGTGCA GACTATCCAG CCCAAGAGGG ACTCCTGAGC

751 TGCCCACATG GCCTAAGATG TGGGTCCTGG ATCCTTCCCC CTTCTCACCA

801 TAACCCCCTC TCAGTGTTTC CCCAACTTCT CCCTTTAGAG CCCAACTCCA

851 GGTCAAATCT GGAGCTCAAA TCCCAGTGCT CCCTCCCCAG GAGTGGGGCC

901 CCAACTCTTC CAAGATACCA GCATTCCTCA AGTCCTCCCA AAACTTCCTA

951 CCCACACCCT CTTCCCAAGG CCCTCAGGGG CAGAAAACAT CTCCTTCAAC

1001 CCGTCCCCAC TCCTTCCTCT GCATGACCTT GGGCAAACCC TTGCCCTTTC

1051 AAGCCATCAG CTCCTGCCTC TCTGCCATGA GGGCTTTGGA TCAGATTCCT

1101 CTTCTCGCCA GGATGAGGAC ACGCACTGCC CTCCATAGAC ACAGATGAAG

1151 GGGTGGGGGT CATTCAGCTC GAATGGGTCC CAGATGCTCA CTTGGCCTTT

1201 CCCTGCAGGA TGAGTGAAGA CGTTTGCCTC TCACAGTGTG TCTTCTACCT

1251 GCATTTTGGC ATCAGAGCCC CCCAGCCCAC CCACCACAGG CAATTACTAG

1301 CCCTAGTTGA TAGGTGAGGT GGGTGAAGAA GGCTGGAGGT GACATGTCCG

1351 AGGTCACACA ACAAAGCAGC ATGCAGGAAC TAGAAACACA TCTTCAGCCT

1401 CCTCCTGGGC CAGCTCTTGT GCTACAGGTG GGGCGGAGCC AGCCCCTCAC

1451 CTTCCTGGTT CCCTGAGGGT CCTCAGGGTG GAGGACAGGT TTGGCCCAGA

1501 AAGACTAGCC AGAGGCCTGA TGGTCCCAGG TGGCTCTGGA TATACTTTGG

1551 ATATGGATTT AAATGGTCTC TAAGAGCCGG GGGTAGGGGG CAGGAAAAGT

1601 GGGTTGTCTT TGCCCCTCAA AGTCCACCTA CCTAGAAACC AAGCCCACGG

1651 TCTTGGCCGT GACCCTGATA ATAAATGGGC TCTCTCAGAG GCGCCAGCCC

1701 CTCCCTCCCC AGCCGGAGGC GTCATCTCTC TTCTGTACCA CTAGAGGGAG

1751 CTCTGATGCA GCTGGAGAGC AGCGCTCAAG GCTCTCGCCC CTCCCCTCCC

1801 TAACCCTTAC CTTCAGTCTC CACCAGCCTG AAGGGCCTCC TAGGGGATCC

1851 TCAGGCGGCC CCCACCAGGG CACACCCTAC TGTCCTTGTG CCTCACGCCC

1901 CCTCCTCATC CTGCACCCCT TCCATCCCAC CTTCCCTTTC AATAAACAGC

1951 TGGGATGGAA AAAAAAAAAA AGAAAAAAAA AAAAAAAAAA AAAAAAAAAA

2001 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AA BLAST Results

Entry HS417348 from database EMBL: human STS WI-14697.

Length = 290

Minus Strand HSPs:

Score = 1254 (188.2 bits), Expect = 3.0e-50, P = 3.0e-50

Identities = 262/273 (95%)

Medline entries

97334404:

A newly identified membrane protein localized exclusively m intracellular organelles of neurons.

Peptide information for frame 2

ORF from 134 bp to 745 bp; peptide length: 204 Category: similarity to known protein

1 MMPSCNRSCS CSRGPSVEDG KWYGVRSYLH LFYEDCAGTA LSDDPEGPPV

51 LCPRRPWPSL CWKISLSSGT LLLLLGVAAL TTGYAVPPKL EGIGEGEFLV

101 LDQRAADYNQ ALGTCRLAGT ALCVAAGVLL AICLFWAMIG WLSQDTKAEP

151 LDPEADSHVE VFGDEPEQQL SPIFRNASGQ SWFSPPASPF GQSSVQTIQP

201 KRDS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_19f19, frame 2

TREMBL:MMP2000_1 product: "P24 protein"; Mouse mRNA for P24 protein, complete eds., N = 1, Score = 295, P = 3.8e-26

>TREMBL:MMP2000_1 product: "P24 protein" Mouse mRNA for P24 protein, complete eds.

Length = 196

HSPs:

Score = 295 (44.3 bits), Expect = 3.8e-26, P = 3.8e-26 Identities = 58/139 (41%), Positives = 81/139 (58%)

Query: 2 MPSCNRSCSCSRGPSVEDGKW YGVRSYLHLFYEDCAGTALSDDPEGPPVLCPRRPWP 58

M SC+ +C R + +G + YGVRSYLH FYEDC + + + P R W Sbjct: 1 MTSCSNTCGSRRAQADTEGGYQQRYGVRSYLHQFYEDCTASIWEYEDDFQIQRΞPNR-WS 59 Query: 59 SLCWKISLSSGTLLLLLGVAALTTGYAVPPKLEGIGEGEFLVLDQRAADYNQALGTCRLA 118 S+ WK+ L SGT+ ++LG+ L G+ VPPK+E GE +F+V+D A YN AL TC+LA Sbjct: 60 ΞVFWKVGLIΞGTVFVILGLTVLAVGFLVPPKIEAFGEADFMVVDTHAVKYNGALDTCKLA 119 Query: 119 GTALCVAAGVLLAICLFWAM 138 G L G +A CL ++ Sbjct: 120 GAVLFCIGGTSMAGCLLMSV 139

Pedant information for DKFZphutel_19f19, frame 2

Report for DKFZphutel_19f19.2

[LENGTH] 204

[MW] 21983.07

[pi] 4.69

[HOMOL] TREMBL :MMP2000_1 product: "P24 protein"; Mouse mRNA for P24 protein, complete cds. 7e-19

[PROSITE] MYRISTYL 4 [PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 3

[PROSITE] PKC_PHOSPHO_SITE 1

[PROSITE] ASN_GLYCOSYLATION 2

[KW] TRANSMEMBRANE 2

[KW] LOW_COMPLEXITY 10.29 %

SEQ MMPSCNRSCSCSRGPΞVEDGKWYGVRSYLHLFYEDCAGTALSDDPEGPPVLCPRRPWPSL

SEG

PRD cccccccccccccccccccccceeehhhhhccccccccccccccccccccccccccccce

MEM MM

SEQ CWKISLSSGTLLLLLGVAALTTGYAVPPKLEGIGEGEFLVLDQRAADYNQALGTCRLAGT

SEG .... xxxxxxxxxxxxxxxxxxxxx

PRD eeeeeccccceeecccceeeecccccccccccccccceeeecccccccchhhhhhhhchh

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMM MMMMMMM

SEQ ALCVAAGVLLAICLFWAMIGWLSQDTKAEPLDPEADSHVEVFGDEPEQQLSPIFRNASGQ

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhccccccccccccccceeeeccccccccccccccccccc

MEM MMMMMMMMMMMMMMMMMMMMMM

SEQ SWFSPPASPFGQSSVQTIQPKRDS

SEG

PRD ccccccccccccceeeeccccccc

MEM

Prosite for DKFZphutel_19f19.2

PS00001 6->10 ASN_GLYCOSYLATION PDOC00001 PS00001 176->180 ASN_GLYCOSYLATION PDOC00001 PS00004 201->205 CAMP_PHOSPHO_SITE PDOC00004 PS00005 114->117 PKC_PHOSPHO_SITE PDOC00005 PS00006 16->20 CK2_PHOSPHO_SITE PDOC00006 PS00006 146->150 CK2_PHOSPHO_SITE PDOC00006 PS00006 157->161 CK2_PHOSPHO_SITE PDOC00006 PS00008 38->44 MYRISTYL PDOC00008 PΞ00008 92->98 MYRISTYL PDOC00008 PS00008 119->125 MYRISTYL PDOC00008 PS00008 127->133 MYRISTYL PDOC00008

(No Pfam data available for DKFZphutel_19f19.2 )

DKFZphutel_19gl9

group: uterus derived

DKFZphutel_19gl9 encodes a novel 400 ammo acid protein, with strong but partial similarity to a bovine elastin-related protein expressed m fetal calf ligamentum nuchae.

The novel protein contains 2 RGD cell attachment sites.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of uterus-specific genes and as a new marker for uterine cells. similarity to bovine elastin fragment complete cDNA, complete eds, EST hits

Sequenced by AGOWA

Locus: map=54.9 cR from top of Chr3 linkage group

Insert length: 3244 bp

Poly A stretch at pos. 3227, polyadenylation signal at pos. 3216

1 GTAACTGCAG TAAGTCCCGC TTGGCCCTGG AGTCCACGCG GATTTTCGAA

51 GCTGGGGCTG GCAAGAGGCC GCTGGACACC ACGCTCCAGT CGTCAGCCCA

101 CTTCCTAGCT GAACAGCGCG AGGCGGCGGC AGCGAGCCGG GTCCCACCAT

151 GGCCGCGAAT TATTCCAGTA CCAGTACCCG GAGAGAACAT GTCAAAGTTA

201 AAACCAGCTC CCAGCCAGGC TTCCTGGAAC GGCTGAGCGA GACCTCGGGT

251 GGGATGTTTG TGGGGCTCAT GGCCTTCCTG CTCTCCTTCT ACCTAATTTT

301 CACCAATGAG GGCCGCGCAT TGAAGACGGC AACCTCATTG GCTGAGGGGC

351 TCTCGCTTGT GGTGTCTCCT GACAGCATCC ACAGTGTGGC TCCGGAGAAT

401 GAAGGAAGGC TGGTGCACAT CATTGGCGCC TTACGGACAT CCAAGCTTTT

451 GTCTGATCCA AACTATGGGG TCCATCTTCC GGCTGTGAAA CTGCGGAGGC

501 ACGTGGAGAT GTACCAATGG GTAGAAACTG AGGAGTCCAG GGAGTACACC

551 GAGGATGGGC AGGTGAAGAA GGAGACGAGG TATTCCTACA ACACTGAATG

601 GAGGTCAGAA ATCATCAACA GCAAAAACTT CGACCGAGAG ATTGGCCACA

651 ATAACCCCAG TGCCATGGCA GTGGAGTCAT TCACGGCAAC AGCCCCCTTT

701 GTCCAAATTG GCAGGTTTTT CCTCTCGTCA GGCCTCATCG ACAAAGTCGA

751 CAACTTCAAG TCCCTGAGCC TATCCAAGCT GGAGGACCCT CATGTGGACA

801 TCATTCGCCG TGGAGACTTT TTCTACCACA GCGAAAATCC CAAGTATCCA

851 GAGGTGGGAG ACTTGCGTGT CTCCTTTTCC TATGCTGGAC TGAGCGGCGA

901 TGACCCTGAC CTGGGCCCAG CTCACGTGGT CACTGTGATT GCCCGGCAGC

951 GGGGTGACCA GCTAGTCCCA TTCTCCACCA AGTCTGGGGA TACCTTACTG

1001 CTCCTGCACC ACGGGGACTT CTCAGCAGAG GAGGTGTTTC ATAGAGAACT

1051 AAGGAGCAAC TCCATGAAGA CCTGGGGCCT GCGGGCAGCT GGCTGGATGG

1101 CCATGTTCAT GGGCCTCAAC CTTATGACAC GGATCCTCTA CACCTTGGTG

1151 GACTGGTTTC CTGTTTTCCG AGACCTGGTC AACATTGGCC TGAAAGCCTT

1201 TGCCTTCTGT GTGGCCACCT CGCTGACCCT GCTGACCGTG GCGGCTGGCT

1251 GGCTCTTCTA CCGACCCCTG TGGGCCCTCC TCATTGCCGG CCTGGCCCTT

1301 GTGCCCATCC TTGTTGCTCG GACACGGGTG CCAGCCAAAA AGTTGGAGTG

1351 AAAAGACCCT GGCACCCGCC CGACACCTGC GTGAGCCCTA GGATCCAGGT

1401 CCTCTCTCAC CTCTGACCCA GCTCCATGCC AGAGCAGGAG CCCCGGTCAA

1451 TTTTGGACTC TGCACCCCCT CTCCTCTTCA GGGGCCAGAC TTGGCAGCAT

1501 GTGCACCAGG TTGGTGTTCA CCAGCTCATG TCTTCCCCAC ATCTCTTCTT

1551 GCCAGTAAGC AGCTTTGGTG GGCAGCAGCA GCCATGAATG GCAAGCTGAC

1601 AGCTTCTCCT GCTGTTTCCT TCCTCTCTTG GACTGAGTGG GTACGGCCAG

1651 CCACTCAGCC CATTGGCAGC TGACAACGCA GACACGCTCT ACGGAGGCCT

1701 GCTGATAAAG GGCTCAGCCT TGCCGTGTGC TGCTTCTCAT CACTGCACAC

1751 AAGTGCCATG CTTTGCCACC ACCACCAAGC ACATCTGTGA TCCTGAAGGG

1801 CGGCCGTTAG TCATTACTGC TGAGTCCTGG GTCACCAGCA GACACACTGG

1851 GCATGGACCC CTCAAAGCAG GCACACCCAA AACACAAGTC TGTGGCTAGA

1901 ACCTGATGTG GTGTTTAAAA GAGAAGAAAC ACTGAAGATG TCCTGAGGAG

1951 AAAAGCTGGA CATATACTGG GCTTCACACT TATCTTATGG CTTGGCAGAA

2001 TCTTTGTAGT GTGTGGGATC TCTGAAGGCC CTATTTAAGT TTTTCTTCGT

2051 TACTTTGCTG CTTCATGTGT ACTTTCCTAC CCCAAGAGGA AGTTTTCTGA

2101 AATAAGATTT AAAAACAAAA CAAAAAAAAC ACTTAATATT TCAGACTGTT

2151 ACAGGAAACA CCCTTTAGTC TGTCAGTTGA ATTCAGAGCA CTGAAAGGTG

2201 TTAAATTGGG GTATGTGGTT TGATTGATAA AAAGTTACCT CTCAGTATTT

2251 TGTGTCACTG AGAAGCTTTA CAATGGATGC TTTTGAAACA AGTATCAGCA

2301 AAAGGATTTG TTTTCACTCT GGGAGGAGAG GGTGGAGAAA GCACTTGCTT

2351 TCATCCTCTG GCATCGGAAA CTCCCCTATG CACTTGAAGA TGGTTTAAAA

2401 GATTAAAGAA ACGATTAAGA GAAAAGGTTG GAAGCTTTAT ACTAAATGGG

2451 CTCCTTCATG GTGACGCCCC GTCAACCACA ATCAAGAACT GAGGCCTGAG

2501 GCTGGTTGTA CAATGCCCAC GCCTGCCTGG CTGCTTTCAC CTGGGAGTGC

2551 TTTCGATGTG GGCACCTGGG CTTCCTAGGG CTGCTTCTGA GTGGTTCTTT

2601 CACGTGTTGT GTCCATAGCT TTAGTCTTCC TAAATAAGAT CCACCCACAC 2651 CTAAGTCACA GAATTTCTAA GTTCCCCAAC TACTCTCACA CCCTTTTAAA

2701 GATAAAGTAT GTTGTAACCA GGATGTCTTA AATGATTCTT TGTGTACCTT

2751 TTCTGTCATA TTCAGAAACC GTTTTGTGCC TGCTGGGAGT AATTCCTTTA

2801 GCAATTAAGT ATTTGGTAGC TGAATAAGGG GTCAGAACTT CTGAAACCAG

2851 AGATCTGTAA TCATCTCTAT TGGCCTGGGG TGCCTGTGCT ATAAATGAGT

2901 TTCTTCACAT GAAAAACACA GCCAGCCCAA GATGACTTAT CTGGGTTTAG

2951 GATTCAATAG TATTCACTAA CTGCTTATTA CATGAGCAAT TTCATCAAAT

3001 CTCCAAACTC TTAAAGGATG CTTTCGGAAA ACACGCTGTA TACCTAGATG

3051 ATGACTAAAT GCAAAATCCT TGGGCTTTGG TTTTTTTCTA GTAAGGATTT

3101 TAAATAACTG CCGACTTCAA AAGTGTTCTT AAAACGAAAG ATAATGTTAA

3151 GAAAAATTTG AAAGCTTTGG AAAACCAAAT TTGTAATATC ATTGTATTTT

3201 TTATTAAAAG TTTTGTAATA AATTTCTAAA AAAAAAAAAA AAAA

BLAST Results

Entry HS545355 from database EMBL: human STS WI-14815.

Length = 436

Minus Strand HSPs:

Score = 2040 (306.1 bits), Expect = 6.2e-86, P = 6.2e-86

Identities = 420/426 (98%)

Entry HS932147 from database EMBL: human STS WI-8531.

Length = 341

Minus Strand HSPs:

Score = 1705 (255.8 bits), Expect = 4.7e-70, P = 4.7e-70

Identities = 341/341 (100%)

Medline entries

86051793:

Bovine elastm cDNA clones: evidence for the occurrence of a new elastin-related protein in fetal calf ligamentum nuchae.

Peptide information for frame 2

ORF from 149 bp to 1348 bp; peptide length: 400 Category: similarity to known protein

1 MAANYSSTST RREHVKVKTS SQPGFLERLS ETSGGMFVGL MAFLLSFYLI

51 FTNEGRALKT ATSLAEGLSL VVΞPDSIHSV APENEGRLVH IIGALRTSKL

101 LSDPNYGVHL PAVKLRRHVE MYQWVETEES REYTEDGQVK KETRYSYNTE

151 WRSEIINSKN FDREIGHNNP SAMAVESFTA TAPFVQIGRF FLSSGLIDKV

201 DNFKSLSLSK LEDPHVDIIR RGDFFYHSEN PKYPEVGDLR VSFSYAGLSG

251 DDPDLGPAHV VTVIARQRGD QLVPFSTKSG DTLLLLHHGD FSAEEVFHRE

301 LRSNSMKTWG LRAAGWMAMF MGLNLMTRIL YTLVDWFPVF RDLVNIGLKA

351 FAFCVATSLT LLTVAAGWLF YRPLWALLIA GLALVPILVA RTRVPAKKLE

BLASTP hits

Entry 145887 from database PIR: elastm - bovine (fragment)

Length = 40

Score = 131 (46.1 bits), Expect = 4.9e-08, P = 4.9e-08

Identities = 31/41 (75%), Positives = 34/41 (82%)

Alert BLASTP hits for DKFZphutel_19gl9, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphutel_19gl9, frame 2

Report for DKFZphutel_19gl9.2

[LENGTH] 400 [MW] 44831.53

[pi^] 7.23

[HOMOL] PIR:I45887 elastin bovine (fragment) le-06

[PROSITE] RGD 2

[PROSITE] MYRISTYL 3

[PROSITE] CAMP_PHOSPHO_SITE

[PROSITE] CK2_PHOSPHO_SITE

[PROSITE] TYR_PHOSPHO_SITE

[PROSITE] PKC_PHOSPHO_SITE

[PROSITE] ASN_GLYCOSYLATION

[KW] TRANSMEMBRANE 4

SEQ MAANYSSTSTRREHVKVKTSSQPGFLERLSETSGGMFVGLMAFLLSFYLIFTNEGRALKT PRD ccceeecccceeeeeeeecccccceeeecccccccchhhhhhhhhhheeeeecccchhhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM..

SEQ ATSLAEGLSLVVSPDSIHSVAPENEGRLVHIIGALRTSKLLSDPNYGVHLPAVKLRRHVE PRD hhhhhccceeeeccccceeeeccccceeeeeeeeeeceeeccccccccccchhhhhhhhh MEM

SEQ MYQWVETEEΞREYTEDGQVKKETRYSYNTEWRSEIINSKNFDREIGHNNPSAMAVESFTA

PRD hheeehhhhheeecccccccceeeccccccceeeeeeccccceeecccccceeeeeeecc

MEM M

SEQ TAPFVQIGRFFLSSGLIDKVDNFKSLΞLSKLEDPHVDIIRRGDFFYHSENPKYPEVGDLR

PRD ccceeeeeeeeeccccccccccceeeeeeeccccceeeeecccceeecccccccccccee

MEM MMMMMMMMMMMMMMMMM

SEQ VSFSYAGLSGDDPDLGPAHVVTVIARQRGDQLVPFSTKSGDTLLLLHHGDFSAEEVFHRE PRD eeccccccccccccccceeeeeeeeecccccccccccccceeeeeecccccchhhhhhhh MEM

SEQ LRSNSMKTWGLRAAGWMAMFMGLNLMTRILYTLVDWFPVFRDLVNIGLKAFAFCVATSLT

PRD hhccccccccchhhhhhhhhhhchhhhhhhhheeecccccccccccceeeeeeeeehhhh

MEM MMMMMMMMMMMMMMMMMMMMMMMM MMMM

SEQ LLTVAAGWLFYRPLWALLIAGLALVPILVARTRVPAKKLE

PRD hhhhhccceeehhhhhhhhhhhhchhhhhhhhcccccccc

MEM MMMMMMMMMMMMMMMMMMMMMMMMM

Prosite for DKFZphutel_19gl9.2

PS00001 4->8 ASN_GLYCOSYLATION PDOC00001 PS00004 140-5-144 CAMP_PHOSPHO_SITE PDOC00004 PS00005 9->12 PKC_PHOSPHO_SITE PDOC00005 PS00005 10->13 PKC_PHOSPHO_SITE PDOC00005 PS00005 97->100 PKC_PHOSPHO_SITE PDOC00005 PS00005 276->279 PKC_PHOSPHO_SITE PDOC00005 PS00005 305->308 PKC_PHOSPHO_SITE PDOC00005 PS00006 10->14 CK2_PHOSPHO_SITE PDOC00006 PS00006 63->67 CK2_PHOSPHO_SITE PDOC00006 PS00006 209->213 CK2_PHOSPHO_SITE PDOC00006 PS00006 249->253 CK2_PHOSPHO_SITE PDOC00006 PS00006 292->296 CK2_PHOSPHO_SITE PDOC00006 PS00006 332->336 CK2_PHOSPHO_SITE PDOC00006 PS00007 220->227 TYR_PHOSPHO_SITE PDOC00007 PS00007 99->107 TYR_PHOSPHO_SITE PDOC00007 PS00008 35->41 MYRISTYL PDOC00008 PS00008 93->99 MYRISTYL PDOC00008 PS00008 310->316 MYRISTYL PDOC00008 PS00016 221->224 RGD PDOC00016 PS00016 268->271 RGD PDOC00016

(No Pfam data available for DKFZphutel_19gl9.2) DKFZphutel_19g22

group: cell structure and motility

DKFZphutel_19g22 encodes a novel 390 ammo acid protein with very strong similarity to tuftelin/enamelin .

Tuftelin/enamelin are matrix proteins of the teeth. As other proteins involved m calcification, these proteins are also expressed in the uterus matrix.

The new protein can find application in modulation of tissue-calcification, especially the uterus . complete cDNA, complete eds start at Bp 51, EST hits in 3' UTR, human homolog of mouse tuftelin tuftelin is descriebed as a matrix protein of teeth but it seems also to be pressend in the uterus matrix

Sequenced by AGOWA

Locus : unknown

Insert length: 3110 bp

Poly A stretch at pos. 3093, polyadenylation signal at pos. 3071

1 GCAGACAGCG GGGTGGACAA GTGGCGTGTG TGCTGCGACC CCGAGGGAAG

51 ATGAACGGGA CGCGGAACTG GTGTACCCTG GTGGACGTGC ACCCAGAGGA

101 CCAGGCGGCG GGCAGCGTGG ACATTCTCAG GCTGACTCTC CAGGGTGAAC

151 TGACAGGAGA TGAACTTGAA CACATAGCCC AGAAGGCGGG CAGGAAGACC

201 TATGCCATGG TGTCCAGCCA CTCAGCTGGT CATTCTCTGG CTTCAGAACT

251 GGTGGAGTCC CATGATGGAC ATGAGGAGAT CATTAAGGTG TACTTGAAGG

301 GGAGGTCTGG AGACAAGATG ATTCACGAGA AGAATATTAA CCAGCTGAAG

351 AGTGAGGTCC AGTACATCCA GGAGGCCAGG AACTGCCTAC AGAAGCTCCG

401 GGAGGATATA AGTAGCAAGC TTGACAGGAA CCTAGGAGAT TCTCTCCATC

451 GACAGGAGAT ACAGGTGGTG CTAGAAAAGC CAAATGGCTT TAGTCAGAGT

501 CCCACAGCCC TGTACAGCAG CCCACCTGAG GTGGACACCT GTATAAATGA

551 GGATGTTGAG AGCTTGAGGA AGACGGTGCA GGACTTGCTG GCCAAGCTTC

601 AGGAGGCCAA GCGGCAACAC CAGTCAGACT GTGTGGCTTT TGAGGTCACA

651 CTCAGCCGGT ACCAGAGGGA AGCAGAACAA AGTAATGTGG CCCTTCAGAG

701 AGAGGAGGAC AGAGTGGAGC AGAAAGAGGC AGAAGTCGGA GAGCTGCAGA

751 GGCGCTTGCT AGGGATGGAG ACGGAGCATC AGGCCTTACT GGCGAAAGTG

801 AGGGAAGGGG AGGTGGCCCT AGAGGAACTT CGGAGCAACA ATGCTGACTG

851 CCAAGCAGAA CGAGAAAAGG CTGCTACCCT GGAAAAGGAA GTGGCCGGGT

901 TGCGGGAGAA GATCCACCAC TTGGATGACA TGCTCAAGAG CCAGCAGCGG

951 AAAGTCCGGC AAATGATAGA GCAGCTCCAG AATTCAAAAG CTGTGATCCA

1001 GTCAAAGGAC GCCACCATCC AGGAGCTCAA GGAGAAAATC GCCTATCTGG

1051 AGGCAGAGAA TTTAGAGATG CATGACCGGA TGGAACACCT GATAGAAAAA

1101 CAAATCAGTC ATGGCAACTT CAGCACCCAG GCCCGGGCCA AGACAGAGAA

1151 CCCGGGCAGT ATTAGGATAT CCAAGCCGCC TAGCCCGAAG CCCATGCCTG

1201 TCATCCGAGT GGTGGAAACC TGAGCTGCCT GGAGATGGTT GCTGCCATTG

1251 CTGCTGCCTC TGCCTCGGAG AAGCCCACTG CCCCTGTTGG CTGTTAACAC

1301 TGCCTTTGAC TTCCTGACTG TCCCCTGGCT GCACCCAGGA CTTCGGGCTC

1351 CTGTGTCTCA CCATTCCCAA GCCCCTGGCC ACTCTAAGCT GGGCAGACGG

1401 AGCACGAGCA CCTATTCAAG GCACTGCAGC CCTTTGGAAG ACATTGTCCT

1451 GCAAGCAGGA GCCAGGGCAA TATCTATATT CCTACAGTGA CTATTTTTCT

1501 CTGTAGAGAG CCTCCCTTCT GTTGTAGACT GGACTCTGGC TGCGCCATAA

1551 GCCAGGCCTT CATCAGATTG GGAGAGGTGA CAAGATTTGC CTCAGCCCTA

1601 AAAGCTGGAG ACACAGATGT CCAGAGTGAT TGGAGAATGT CCTGGGGGAA

1651 TGAAGTTCCT TCCACAAACA CAGCTCAGTT CTTAGCAACA AACTGTTTGT

1701 TTTTCTACTT GCTCCATCTG CAGCCTACGC TGCCCTGGCC TCCTGCAGAC

1751 AGATAGTGGG GTTACCTGGC AAGGCCTGGT GAGAGCCAGT GAACCTAAGC

1801 TTTGACTGGG TGGCCTTGTC TTTCTGGGGA GGAGGGAATG TACATTCAGG

1851 GAGTAGCCTT TTGCGGAAAA ATTCTCTAGG GCTACAGACA GTCATGTGTG

1901 ACTTCTCTCT GCTGTGAAAA CTCCCAGAGT CTCTTTAGGG ATTTTCCCTA

1951 AGGTGTACCA CCAGGCACAC CTCAGTCTTC TTGACCCAGA GCCTGAAAAC

2001 TGTTTTCACT GGGTTCCACC AGTCCCAGCA AAATCCTCTT TGTATTTATT

2051 TTGCTAAGTT ATTGGTGGTT TTGCTTACAT CTCATGATTG ATATAATACC

2101 AAAGTTCTAT AGCCTTCTCT TGCAGTATTT GGATTTGCTT GAAACCGGGA

2151 AAACTGTTCC CATTAGGCTT GTTAATGTCA GAGTGACACT ATTATGAATC

2201 TTTCTCTCCC TTTCCTCTGC CTGTTTCTTC TCTCTTTCTC CTTCAAACTT

2251 GCTCTGCAGC TAAGGAAGGT GAGTCTACTT TCCCTGAGGC TTTGGGGTCA

2301 GAGTATATGT TGTTTGGAGA AAGAGGGCAA TCAGGACTCT' TCTGGGACCC

2351 AGATGAGTTC TTCACTAGCC CTTCTGAACC CCTTGCTCCA TAATTGGTCT

2401 TTTATCCTGG CTCTGAATGA CCCTGCAGGT CATCATGGTT TTCTTTTTTT

2451 ATTGTTTTTT TTTTTTTCTG AGACAGAGTC TCACTCTGTC ACCCAGGCTG

2501 GAGTGCAGTG GCGCGATCTC AGCTCACTGC AACCTCTGCC TCCCGGATTT

2551 AAGCGATTCT TCTGCCTCAG CCTCCCGAGT AGCTGGGACT ACAGGTGTGC 2601 CACCACGCCT GGCTGATTTT TGTATTTTTA GTAGAGATGG GGTTTCACCA

2651 TACTGGCTAG GCTGGTCTCG AATTCCTGAC CTCAGGTGAT CCACCCACCT

2701 CGGCTTCCCA AAGTGCTAGG ATTATAGGCT TGAGCTACTG TGCCCGGCCC

2751 ATGGTGTTTT TCTTTAGGGC TCTTCCTACA GCCTTGAGAA GTAGATAGGC

2801 ATCAGAGTAT GGTACTATAG GAATCAGAAA AATTCAAAAC AAATGTGGAT

2851 TAAGTGTTTA GGCTCTATGT GGCTCACGCA GCCAGAATCC TTAAGTCTGT

2901 GTGTTTCTGT GTCTCAAGAC TGGGCTCACA TTCTGGCTTT GTCCATAACA

2951 ATGCTCTGGG ATTTCAGGGA GTTCCCTCAT TTGTAAAATG AGGGGGTCAG

3001 AGCAGGTGAT ATCCATGTTT CTTCCCTTTC TGATATTGTT GTCTGTGGCA

3051 TATTCTTTGT ATGGCGAATT TAATAAATTA TATTAATGTG TCTAAAAAAA

3101 AAAAAAAAAA

BLAST Results

No BLAST result

Medline entries

98200312:

Tuftelιn--aspects of protein and gene structure

97228909:

Timing of the expression of enamel gene products during mouse tooth development .

91340750:

Sequencing of bovine enamelm ("tuftelin") a novel acidic enamel protein.

Peptide information for frame 3

ORF from 51 bp to 1220 bp; peptide length: 390 Category: strong similarity to known protein

1 MNGTRNWCTL VDVHPEDQAA GSVDILRLTL QGELTGDELE HIAQKAGRKT

51 YAMVSSHSAG HSLASELVES HDGHEEIIKV YLKGRSGDKM IHEKNINQLK

101 SEVQYIQEAR NCLQKLREDI SSKLDRNLGD SLHRQEIQVV LEKPNGFSQS

151 PTALYSSPPE VDTCINEDVE SLRKTVQDLL AKLQEAKRQH QSDCVAFEVT

201 LSRYQREAEQ SNVALQREED RVEQKEAEVG ELQRRLLGME TEHQALLAKV

251 REGEVALEEL RSNNADCQAE REKAATLEKE VAGLREKIHH LDDMLKSQQR

301 KVRQMIEQLQ NSKAVIQSKD ATIQELKEKI AYLEAENLEM HDRMEHLIEK

351 QISHGNFSTQ ARAKTENPGS IRISKPPSPK PMPVIRVVET

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphutel_19g22, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphutel_19g22, frame 3

Report for DKFZphutel_19g22.3

[LENGTH] 390 [MW] 44264.09

[pi] 5.68

[HOMOL] TREMBL:AF047704_1 product: "tuftelin"; Mus musculus tuftelin mRNA, complete cds. 0.0

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL058w]

2e-ll

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDL058w] 2e-ll

[ FUNCAT] 1 genome replication, transcription, recombination and repair [M. jannaschn, MJ1643] 7e-ll

[FUNCAT] 09.13 biogenesis of chromosome structure [S. cerevisiae, YLR086w] le-08

[FUNCAT] 03.22.01 cell cycle check point proteins [S. cerevisiae, YGL086w] 6e-08

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YGL086w] 6e-08

[ FUNCAT] 03.13 meiosis [S. cerevisiae, YNL250w] 7e-08 [FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YNL250w] 7e-08

[FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YKR095w] le-07

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YDR285w] 2e-07

[FUNCAT] 30.13 organization of chromosome structure [S. cerevisiae, YDR285w] 2e-07

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YOR216c] le-05

[FUNCAT] 01.03.16 polynucleotide degradation [S. cerevisiae, YNL243w] le-04

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YNL243w] le-04

[FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YNL243w] le-04

[FUNCAT] 03.07 pheromone response, mat g-type determination, sex-specific proteins

[S. cerevisiae, YNL243w] le-04

[FUNCAT] 08.19 cellular import [S. cerevisiae, YNL243w] le-04

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YNL243w] le-04

[FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YHR023w MYOl - myosιn-1 isoform] 4e-04

[FUNCAT] 03.25 cytokinesis [S. cerevisiae, YHR023w MYOl - myosιn-1 isoform] 4e-04

[FUNCAT] 09.10 nuclear biogenesis [S. cerevisiae, YDR356w] 4e-04

[FUNCAT] 30.05 organization of centrosome [S. cerevisiae, YMR294w] 7e-04

[EC] 3.6.1.32 Myosin ATPase 8e-09

[PIRKW] blocked amino end le-07

[PIRKW] nucleus le-06

[PIRKW] citrulline le-07

[PIRKW] tandem repeat 8e-09

[PIRKW] heterodimer 3e-06

[PIRKW] DNA repair 2e-06

[PIRKW] heart 8e-09

[PIRKW] endocytosis 3e-07

[PIRKW] transmembrane protein 4e-10

[PIRKW] zinc fmger 3e-07

[PIRKW] metal binding 3e-07

[PIRKW] muscle contraction 8e-09

[PIRKW] acetylated amino end le-06

[PIRKW] actin binding 8e-09

[PIRKW] microtubule binding le-06

[PIRKW] cell division control le-06

[PIRKW] ATP 8e-09

[PIRKW] chromosomal protein 3e-06

[PIRKW] thick filament 8e-09

[PIRKW] phosphoprotem le-145

[PIRKW] skeletal muscle 8e-09

[PIRKW] calcium binding le-07

[PIRKW] meiosis 2e-06

[PIRKW] alternative splicing 7e-08

[PIRKW] DNA condensation 3e-06

[PIRKW] coiled coil 4e-10

[PIRKW] P-loop 8e-09

[PIRKW] heptad repeat le-07

[PIRKW] methylated ammo acid 8e-09

[PIRKW] lmmunoglobulm receptor 2e-06

[PIRKW] peripheral membrane protein 3e-07

[PIRKW] cardiac muscle 8e-09

[PIRKW] hydrolase 8e-09

[PIRKW] muscle 7e-08

[PIRKW] EF hand le-07

[PIRKW] cytoskeleton 7e-08

[PIRKW] hair le-07

[PIRKW] smooth muscle 7e-08

[PIRKW] calmodulin binding 3e-07

[SUPFAM] conserved hypothetical P115 protein 2e-09

[SUPFAM] myosin heavy chain 8e-09

[SUPFAM] RAD50 protein 2e-06

[SUPFAM] calmodulin repeat homology le-07

[SUPFAM] myosin motor domain homology 8e-09

[SUPFAM] alpha-actmin actin-binding domain homology le-06

[SUPFAM] tropomyosm 7e-08

[SUPFAM] protein-tyrosine kinase ret 3e-07

[SUPFAM] plectm le-06

[SUPFAM] tπchohyalin le-07

[SUPFAM] pleckstrin repeat homology 2e-06

[SUPFAM] ribosomal protein S10 homology le-06

[SUPFAM] protein kinase homology 3e-07

[SUPFAM] protein kinase C zinc-binding repeat homology 2e-06

[SUPFAM] giantin 4e-06

[SUPFAM] kinesin-related protein KLPA le-06

[SUPFAM] kinesin motor domain homology le-06

[SUPFAM] human early endosome antigen 1 3e-07

[SUPFAM] M5 protein 2e-06

[PROSITE] MYRISTYL 1

[PROSITE] AMIDATION 1

[PROSITE] CK2 PHOSPHO_SITE 6 [PROSITE] PKC PHOSPHO SITE 4

[PROSITE] ASN GLYCOSYLATION 2

[KW] All Alpha

[KW] LOW COMPLEXITY 4.62

[KW] COILED COIL 35.13

SEQ MNGTRNWCTLVDVHPEDQAAGSVDILRLTLQGELTGDELEHIAQKAGRKTYAMVSSHSAG SEG

PRD cccccceeeeeeeccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS

SEQ HSLASELVESHDGHEEIIKVYLKGRΞGDKMIHEKNINQLKSEVQYIQEARNCLQKLREDI

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS

SEQ SSKLDRNLGDSLHRQEIQVVLEKPNGFSQSPTALYSSPPEVDTCINEDVESLRKTVQDLL

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCC

SEQ AKLQEAKRQHQSDCVAFEVTLSRYQREAEQSNVALQREEDRVEQKEAEVGELQRRLLGME

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCC CCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ TEHQALLAKVREGEVALEELRSNNADCQAEREKAATLEKEVAGLREKIHHLDDMLKΞQQR

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CC CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ KVRQMIEQLQNSKAVIQSKDATIQELKEKIAYLEAENLEMHDRMEHLIEKQISHGNFSTQ

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCC CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ ARAKTENPGSIRISKPPSPKPMPVIRVVET

SEG xxxxxxxxxxxxxxxxx ...

PRD hhcccccccceeeecccccccccceeeccc

COILS

Prosite for DKFZphutel_19g22.3

PS00001 2->6 ASN_GLYCOSYLATION PDOC00001

PS00001 356->360 ASN_GLYCOSYLATION PDOC00001

PS00005 121->124 PKC_PHOSPHO_SITE PDOC00005

PS00005 171->174 PKC_PHOSPHO_SITE PDOC00005

PS00005 370->373 PKC_PHOSPHO_SITE PDOC00005

PS00005 378->381 PKC_PHOSPHO_SITE PDOC00005

PS00006 9->13 CK2_PHOSPHO_SITE PDOC00006

PS00006 35->39 CK2_PHOSPHO_SITE PDOC00006

PS00006 122->126 CK2_PHOSPHO_ΞITE PDOC00006

PS00006 157->161 CK2_PHOSPHO_SITE PDOC00006

PS00006 175->179 CK2_PHOSPHO_SITE PDOC00006

PS00006 322->326 CK2_PHOSPHO_SITE PDOC00006

PS00008 355->361 MYRISTYL PDOC00008

PS00009 46->50 AMIDATION PDOC00009

(No Pfam data available for DKFZphutel_19g22.3) DKFZphutel_19hl7

group: intracellular transport and trafficking

DKFZphutel_19hl7 encodes a novel 879 amino acid protein, with similarity to N.crassa osbP oxysterol-bmding protein.

The novel protein contains a oxysterol-binding protein family signature. Mammalian oxysterol- binding protein (OSBP) is a protein binds a variety of oxysterols (oxygenated derivatives of cholesterol) . OSBP seems to play a complex role in the regulation of sterol metabolism. OSBP is a cytosolic/Golgi receptor for oxysterols such as 25-hydroxycholesterol, and thus a potential target of siphingomyelm turnover and cholesterol mobilization at the plasma membrane and/or Golgi apparatus. Therefore, the new protein seems to be involved in oxysterol metabolism.

The new protein can find application in modulating the response of cells to oxysterols. The protein can be used as marker for the golgi system. The Protein might be used to direct drugs to the golgi system m response to oxidative stess. strong similarity to C. elegans ZK1086.1 and oxysterol-binding proteins complete cDNA, complete eds, few EST hits similarity to proteins involved m steroid biosynthesis

Sequenced by AGOWA

Locus: unknown

Insert length: 3828 bp

Poly A stretch at pos. 3811, polyadenylation signal at pos. 3784

1 GCCCGCGCGC CCGGCCGGCC CGGAGCACCG AGCTCGCGGC ACGGTAGGAG

51 AAGCCCCCGA GCGCCCACAG CATGAAGGAG GAGGCCTTCC TCCGGCGCCG

101 CTTCTCCCTG TGTCCACCTT CCTCCACCCC TCAGAAAGTC GACCCCCGGA

151 AGCTCACCCG GAACTTGCTC CTCAGCGGAG ACAATGAGCT CTACCCACTC

201 AGCCCAGGGA AGGACATGGA GCCCAACGGC CCGTCGCTGC CCAGGGATGA

251 AGGGCCCCCG ACCCCAAGCT CTGCCACGAA GGTGCCACCG GCAGAGTACA

301 GGCTGTGCAA CGGGTCAGAC AAGGAATGTG TGTCCCCCAC CGCCAGGGTC

351 ACCAAGAAGG AGACTCTCAA GGCGCAGAAG GAGAACTACC GGCAGGAGAA

401 GAAGCGCGCC ACACGGCAGC TGCTCAGCGC TCTGACAGAC CCCAGCGTGG

451 TCATCATGGC TGACAGCCTG AAGATCCGCG GCACCCTGAA GAGCTGGACC

501 AAGCTGTGGT GCGTGCTGAA GCCGGGGGTG CTGCTCATCT ACAAGACGCC

551 CAAGGTGGGC CAGTGGGTGG GCACGGTGCT GCTGCACTGC TGCGAGCTCA

601 TCGAGCGGCC CTCCAAGAAG GACGGCTTCT GCTTCAAGCT CTTCCACCCG

651 CTGGATCAGT CCGTCTGGGC CGTGAAGGGC CCCAAAGGTG AGAGCGTGGG

701 CTCCATCACA CAGCCCCTGC CCAGCAGCTA CCTGATCTTC AGGGCCGCCT

751 CCGAGTCAGA TGGTCGCTGC TGGCTGGACG CCCTGGAGCT GGCCCTGCGC

801 TGCTCTAGCC TACTGAGACT GGGCACCTGC AAGCCGGGCC GAGACGGGGA

851 GCCAGGGACC TCGCCAGACG CATCACCCTC ATCGCTCTGT GGGCTGCCAG

901 CCTCAGCCAC TGTCCACCCA GACCAAGACC TGTTCCCACT GAACGGGTCT

951 TCCCTGGAGA ACGATGCATT CTCAGACAAG TCGGAGAGAG AGAACCCTGA

1001 GGAGTCAGAT ACCGAGACCC AGGACCATAG CCGGAAGACG GAGAGTGGCA

1051 GCGACCAGTC AGAGACCCCT GGGGCCCCGG TGCGGAGAGG GACCACCTAT

1101 GTGGAGCAGG TCCAGGAGGA GCTGGGGGAG CTGGGCGAGG CGTCCCAGGT

1151 GGAGACAGTG TCAGAGGAGA ACAAGAGTCT GATGTGGACC CTGCTGAAGC

1201 AGCTACGGCC AGGCATGGAC CTGTCCCGCG TGGTGCTACC CACGTTCGTA

1251 CTGGAGCCGC GCTCCTTCCT GAACAAGCTC TCCGACTACT ACTACCACGC

1301 AGACCTGCTC TCCAGGGCTG CGGTGGAGGA GGATGCCTAC AGCCGCATGA

1351 AGCTGGTGCT GCGGTGGTAC CTGTCTGGCT TCTACAAGAA GCCCAAGGGA

1401 ATCAAGAAGC CGTACAACCC CATCCTGGGG GAGACCTTCC GCTGCTGCTG

1451 GTTCCACCCG CAGACTGACA GCCGCACATT CTACATAGCA GAGCAGGTGT

1501 CCCACCACCC GCCCGTGTCT GCCTTCCACG TCAGCAACCG GAAGGACGGC

1551 TTCTGCATCA GTGGCAGCAT CACAGCCAAG TCCAGGTTTT ATGGGAACTC

1601 GCTGTCGGCG CTGCTGGACG GCAAAGCCAC GCTCACCTTC CTGAACCGAG

1651 CCGAGGATTA CACCCTTACC ATGCCCTACG CCCACTGCAA AGGAATCCTG

1701 TATGGCACGA TGACCCTGGA GCTGGGTGGG AAGGTCACCA TCGAGTGTGC

1751 GAAGAACAAC TTCCAGGCCC AGCTGGAATT CAAACTCAAG CCCTTCTTCG

1801 GGGGTAGCAC CAGCATCAAC CAGATCTCGG GAAAGATCAC GTCGGGAGAG

1851 GAAGTCCTGG CGAGCCTCAG TGGCCACTGG GACAGGGACG TGTTTATCAA

1901 GGAGGAAGGG AGCGGAAGCA GTGCGCTTTT CTGGACCCCG AGCGGGGAGG

1951 TCCGCAGACA GAGGCTGAGG CAGCACACGG TGCCGCTGGA GGAGCAGACG

2001 GAGCTGGAGT CCGAGAGGCT CTGGCAGCAC GTCACCAGGG CCATCAGCAA

2051 GGGCGACCAG CACAGGGCCA CACAGGAGAA GTTTGCACTG GAGGAGGCAC

2101 AGCGGCAGCG GGCCCGTGAG CGGCAGGAGA GCCTCATGCC CTGGAAGCCG

2151 CAGCTGTTCC ACCTGGACCC CATCACCCAG GAGTGGCACT ACCGATACGA

2201 GGACCACAGC CCCTGGGACC CCCTGAAGGA CATCGCCCAG TTTGAGCAAG

2251 ACGGGATCCT GCGGACCTTG CAGCAGGAGG CCGTGGCCCG CCAGACCACC 2301 TTCCTGGGCA GCCCAGGGCC CAGGCACGAG AGGTCTGGCC CAGACCAGCG

2351 GCTTCGCAAG GCCAGCGACC AGCCCTCCGG CCACAGCCAG GCCACGGAGA

2401 GCAGCGGATC CACGCCTGAG TCCTGCCCAG AGCTCTCAGA CGAGGAGCAG

2451 GATGGTGACT TTGTCCCTGG CGGTGAGAGC CCATGCCCTC GGTGCAGGAA

2501 GGAGGCGCGG CGGCTGCAGG CCCTGCACGA GGCCATCCTC TCCATCCGAG

2551 AGGCCCAGCA GGAGCTGCAC AGGCACCTCT CGGCCATGCT GAGCTCCACG

2601 GCACGGGCAG CACAGGCACC GACCCCAGGC CTCCTGCAGA GCCCCCGATC

2651 CTGGTTCCTG CTCTGCGTGT TCCTGGCGTG TCAGCTGTTC ATTAACCACA

2701 TCCTCAAATA GGAGCCCTGG GGGCAGAGCT CCTGGCCAGT CCCGAGCCCT

2751 CCCTCCCAGG CACCCAGCAC TTTAAGCCTG CTCCATGGAG GCAGAGAGGC

2801 CCGGCAAGCA CAGCCACTGT GACGGGGAGT CCAGGCGCAG GAGGGACCCG

2851 GGGCCACAAG GCGCTGCGGG CCCAGGTGTG CTGGGCCCCT CTCAGGGGCA

2901 CTGGCCTCTC TGCAGGGCCT TCCGCCCAGC GCTGGCCTTA ATGCTAAAGC

2951 CAAATGCAGC TTCTGCTGTG CGACGCACTC CTGGCCATCT TGCCGTGTCA

3001 CCCCCTGTCC GGCCTCCACT TGCCATGGGG GATGGATGGA TTTAGGGTGG

3051 GAGGGCCTGT GGGGGCCCTG GACAGTCACA CCCCAGCAGC AGTGAGTGGG

3101 CAGGTTTGGA GGAGCAGCCA GGGAGCCCCG AGTGGCCCAG GAGTCCCCCC

3151 ACACACAGAT GCATAGGCCT GCCTTCCGGA GACCCTGTCC ACATTGCCGG

3201 GACCACCCTG GTGGGGCCAC TGGTGGGTGC CAGGGACAGG TTAGGGCCAC

3251 TCTGGGGAAG GCATTTTGGT TTTTTATTCC ACGCTCTGCT GTTTGGATGG

3301 GAGCCCCACA GAGGCAGGTC CTGGAACCAC CCCACCCCCA CACCTGGACG

3351 CTCGCTCTGG TGGGGGCACA CGCAGGTGGA GGTGGTTGTG GGTGCAGGTG

3401 TGTGCAGGGG TGTGGGGGGC GCAGGGGTGT GGCTTAGCTG GCCCCGCACC

3451 CAGGCCGGGG AGGCTCAAGT TCGCCACTTT ACTCAGACCG ATGCACAGTC

3501 TTCCCATTTT ACACTTTTTT AATAAACATA ATTGCAATAT TTTAGGTGGG

3551 CTGCGAGCTG CAGTCAGCCT TCACGTCTGG CCTCAGTCCC CGTGTCAGTG

3601 CCGCTCTGCG TGTGCGTGTG CGCGTGTGTG AGCCTCTACA CATATATATA

3651 TGTACAGAGC CTTAAACCAC ATCGTGGCGG TGCCGTCTGA GCTGTAGCGG

3701 GTGGCTTTGT TTCCAGTTTT TGTACCCGTG TCCTTGTCTC CCCTCCTCCC

3751 CCATCTGGGG ATGTGTCTGT GTTCCACACC TTGAAATAAA CAGACACATA

3801 CGTGTTCTCT TAAAAAAAAA AAAAAAAA

BLAST Results

No BLAST result

Medline entries

98315477:

The pleckstπn homology domain of oxysterol-binding protein recognises a determinant specific to Golgi membranes .

98146266:

A Drosophila homologue of oxysterol binding protein (OSBP) —implications for the role of OSBP.

98146266:

A Drosophila homologue of oxysterol binding protein (OSBP) --implications for the role of OSBP.

Peptide information for frame 3

ORF from 72 bp to 2708 bp; peptide length: 879 Category: strong similarity to known protein

1 MKEEAFLRRR FSLCPPSSTP QKVDPRKLTR NLLLSGDNEL YPLSPGKDME

51 PNGPSLPRDE GPPTPSSATK VPPAEYRLCN GSDKECVSPT ARVTKKETLK

101 AQKENYRQEK KRATRQLLSA LTDPSVVIMA DSLKIRGTLK SWTKLWCVLK

151 PGVLLIYKTP KVGQWVGTVL LHCCELIERP SKKDGFCFKL FHPLDQSVWA

201 VKGPKGESVG SITQPLPSSY LIFRAASESD GRCWLDALEL ALRCSSLLRL

251 GTCKPGRDGE PGTSPDASPS SLCGLPASAT VHPDQDLFPL NGSSLENDAF

301 SDKSERENPE ESDTETQDHS RKTESGSDQS ETPGAPVRRG TTYVEQVQEE

351 LGELGEASQV ETVSEENKSL MWTLLKQLRP GMDLSRVVLP TFVLEPRSFL

401 NKLSDYYYHA DLLSRAAVEE DAYSRMKLVL RWYLSGFYKK PKGIKKPYNP

451 ILGETFRCCW FHPQTDSRTF YIAEQVSHHP PVSAFHVSNR KDGFCISGSI

501 TAKSRFYGNS LSALLDGKAT LTFLNRAEDY TLTMPYAHCK GILYGTMTLE

551 LGGKVTIECA KNNFQAQLEF KLKPFFGGST SINQISGKIT SGEEVLASLS

601 GHWDRDVFIK EEGSGSSALF WTPSGEVRRQ RLRQHTVPLE EQTELESERL 651 WQHVTRAISK GDQHRATQEK FALEEAQRQR ARERQESLMP WKPQLFHLDP

701 ITQEWHYRYE DHSPWDPLKD IAQFEQDGIL RTLQQEAVAR QTTFLGSPGP

751 RHERSGPDQR LRKASDQPSG HSQATESSGS TPESCPELSD EEQDGDFVPG

801 GESPCPRCRK EARRLQALHE AILSIREAQQ ELHRHLSAML SSTARAAQAP

851 TPGLLQSPRS WFLLCVFLAC QLFINHILK

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_19hl7, frame 3

TREMBL :CEZK1086_2 gene: "ZK1086.1"; Caenorhabditis elegans cosmid ZK1086, N = 1, Score = 1495, P = 2.7e-153

PIR:S25324 hypothetical protein YKR003w - yeast (Saccharomyces cerevisiae), N = 2, Score = 574, P = 8.5e-57

TREMBL:CEAF195_7 gene: "C32F10.1"; Caenorhabditis elegans cosmid C32F10., N = 1, Score = 588, P = 8.6e-57

PIR:S46796 hypothetical protein YKR003w homolog YHROOlw - yeast (Saccharomyces cerevisiae), N = 1, Score = 585, P = 1.9e-56

TREMBL :NCOSBP_l gene: "osbP"; product: "oxysterol-binding protein"; N.crassa mRNA for putative oxysterol-binding protein, N = 1, Score = 571, P = 7e-55

TREMBL: AB017026_1 product: "oxysterol-binding protein"; Mus musculus mRNA for oxysterol-b dmg protein, complete eds., N = 2, Score = 328, P = 3e-35

>TREMBL:CEZK1086_2 gene: "ZK1086.1"; Caenorhabditis elegans cosmid ZK1086 Length = 751

HSPs:

Score = 1495 (224.3 bits), Expect = 2.7e-153, P = 2.7e-153 Identities = 327/663 (49%), Positives = 430/663 (64%)

Query: 129 MADSLKIRGTLKSWTKLWCVLKPGVLLIYKTPKV—GQWVGTVLLHCCELIERPSKKDGF 186

MAD+LKIRG LK W + +CVLKPG+L++YK K G WVGTVLL+ CELIERPSKKDGF Sbjct: 1 MADTLKIRGALKRWNRYYCVLKPGLLILYKHKKADRGDWVGTVLLNHCELIERPSKKDGF 60

Query: 187 CFKLFHPLDQSVWAVKGPKGESVGSIT-QPLPSSYLIFRAASESDGRCWLDALELALRCS 245

CFKLFHP+D S+W +GP G+S GS T PL +S+LI RA S+ GRCW+DALEL+ +C+ Sbjct: 61 CFKLFHPMDMSIWGNRGPLGQSFGSFTLNPLNTSFLICRAPSDQAGRCWMDALELSFKCT 120

Query: 246 SLLRLGTCKPGRDGEPGTSPDASPSSLCGLPASATVHPDQDLFPLNGSSLENDAFΞDK-S 304

LL+ T D + G D+S + G + + D D G A Ξ+ + Sbjct: 121 GLLKK-TMNE-LDDKNG DSSMND—GQRDESRMSRDSD GDDTRELAVSETDA 168

Query: 305 ERENPEESDTETQDHSRKTESGSDQSETPGAPVRRGTT YVEQVQEELGELGEAΞQVE 361

E+ E D + +DH E G SET +R T ++ +E G G S E Sbjct: 169 EKHFQEIDDVQDEDH EDGK-MSETSDT-IREAFTESAWIPSPKEVFGPDG—SLTE 220

Query: 362 TVSEENKSLMWTLLKQLRPGMDLSRVVLPTFVLEPRSFLNKLSDYYYHADLLSRAAVEED 421

V EENKSL+WTLLKQ+RPGMDLS+VVLPTF+LEPRSFL KL+DYYYHADL+S A E D Sbjct: 221 EVGEENKSLIWTLLKQIRPGMDLSKVVLPTFILEPRSFLEKLADYYYHADLISEAVAEPD 280

Query: 422 AYSRMKLVLRWYLSGFYKKPKGIKKPYNPILGETFRCCWFHPQTDSRTFYIAEQVSHHPP 481

+ R+ V +++LSGFYKKPKG+KKPYNPILGETFRC W HP S TFY+AEQVSHHPP Sbjct: 281 PFQRIVKVTKFFLSGFYKKPKGLKKPYNPILGETFRCKWEHPD-GSTTFYMAEQVSHHPP 339

Query: 482 VSAFHVSNRKDGFCISGSITAKSRFYGNSLSALLDGKATLTFLNRAEDYTLTMPYAHCKG 541

VS+ ++NRK GF ISG+I AKS++YGNSLSA+L GK LT LN E Y + +PYA+CKG Sbjct: 340 VSSLFITNRKAGFNISGTILAKSKYYGNSLSAILAGKLRLTLLNLGETYIVNLPYANCKG 399

Query: 542 ILYGTMTLELGGKVTIECAKNNFQAQLEFKLKPFFGGSTSINQISGKITSGEEVLASLSG 601

1+ GTMT+ELGG+V IEC K ++ L+FKLKP GG+ NQI G I G + LAS+ G Sbjct: 400 IMIGTMTMELGGEVNIECEKTGYRTTLDFKLKPMLGGA--YNQIEGΞIKYGSDRLASIEG 457

Query: 602 HWDRDVFIKEEGSGSSALFWTPSGEVRRQRLRQHTVPLEEQTELESERLWQHVTRAISKG 661

WD + IK G W P+ EV + RL ++ + ++EQ E ES +LW+HVT AIS Sbjct: 458 AWDGVIRIK—GPDGKKELWNPTPEVIKTRLPRYEINMDEQGEWESAKLWRHVTEAISNE 515

Query: 662 DQHRATQEKFALEEAQRQRARERQESLMPWKPQLFHLDPITQEWHYRYEDHSPWDPLKDI 721

DQ++AT+EK ALE QR RA+ S +P + + F ++ Y + D+ PWD Dl Sbjct: 516 DQYKATEEKTALENDQRARAK SGIPHETKFFKKQH-GDDYVYIHADYRPWDNNNDI 570 Query 722 AQFEQDGILRTLQQEAVAR--QTTFLGSPGPRHERSGPDQRLRKASDQPSGHSQATESSG 779

Q E + +++T+ + + + + LGS E S D+ + +P + + Sbjct 571 QQIENNYVVKTISRHSKRKTGNSEQLGSDNTS-EASESDEEVI EPKIKKKEIVPAK 625 Query 780 STPESCPELSDE 791

S P + PE++DE Sbjct 626 SKPIT-PEVADE 636

Pedant information for DKFZphutel_19hl7, frame 3

Report for DKFZphutel_19hl7.3

[LENGTH] 879 [MW] 98616.79 [pi] 7.29 [HOMOL] TREMBL:CEZK1086_2 gene: 'ZK1086.1"; Caenorhabditis elegans cosmid ZK1086 le-157

[ FUNCAT ] 01.06.16 lipid and fatty-acid binding [S. cerevisiae, YHROOlw] 3e-55

[FUNCAT] 01.06.01 lipid, fatty-acid and sterol biosynthesis [S. cerevisiae, YHROOlw]

3e-55

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YPL145C] 3e-23

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YPL145c]

3e-23

[FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YAR044w] 5e-20

[BLOCKS] BL00168F

[BLOCKS] BL01013D Oxysterol-binding protein family proteins

[BLOCKS] BL01013C Oxysterol-binding protein family proteins

[BLOCKS] BL01013B Oxysterol-binding protein family proteins

[BLOCKS] BL01013A Oxysterol-bmding protein family proteins

[PIRKW] transmembrane protein le-19

[SUPFAM] pleckstrm repeat homology 8e-18

[SUPFAM] ankyrin repeat homology le-19

[SUPFAM] unassigned ankyrin repeat proteins le-19

[PROSITE] MYRISTYL 12

[PROSITE] CAMP_PHOSPHO_SITE 6

[PROSITE] OSBP 1

[PROSITE] CK2_PHOSPHO_SITE 21

[PROSITE] PROKAR_LIPOPROTEIN 1

[PROSITE] TYR_PHOSPHO_SITE 2

[PROSITE] PKC_PHOSPHO_SITE 20

[PROSITE] ASN_GLYCOSYLATION 3

[PFAM] PH (pleckstrm homology) domain

[KW] TRANSMEMBRANE 1

[KW] LOW_COMPLEXITY 2.96 %

[KW] COILED COIL 3.53 %

SEQ MKEEAFLRRRFSLCPPSSTPQKVDPRKLTRNLLLSGDNELYPLSPGKDMEPNGPSLPRDE

SEG

PRD ccchhhhhhhhccccccccccccccccccccccccccccccccccccccccccccccccc

COILS

MEM

SEQ GPPTPSSATKVPPAEYRLCNGSDKECVSPTARVTKKETLKAQKENYRQEKKRATRQLLSA

SEG

PRD cccccccccccccceeeecccccceeeeccccchhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCC

MEM

SEQ LTDPSVVIMADSLKIRGTLKSWTKLWCVLKPGVLLIYKTPKVGQWVGTVLLHCCELIERP

SEG

PRD hcccceeeecccccccccccccceeeeeeccceeeeecccccccceeeeecccccccccc

COILS CCC

MEM

SEQ SKKDGFCFKLFHPLDQSVWAVKGPKGESVGΞITQPLPSSYLIFRAASESDGRCWLDALEL

SEG

PRD ccccceeeeecccccceeeeecccccceeecccccccceeeeeeehhhhhhhhhhhhhhh

COILS

MEM

SEQ ALRCSSLLRLGTCKPGRDGEPGTSPDASPSSLCGLPASATVHPDQDLFPLNGSSLENDAF

SEG

PRD hhhhhhhhhhhhcccccccccccccccccccccccccccccccccccccccccccccccc

COILS

MEM

SEQ SDKSERENPEESDTETQDHSRKTESGSDQSETPGAPVRRGTTYVEQVQEELGELGEASQV SEG xxxxxxxxxxxxx .... PRD cccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhccccccc COILS MEM

SEQ ETVSEENKSLMWTLLKQLRPGMDLSRVVLPTFVLEPRSFLNKLSDYYYHADLLSRAAVEE

SEG

PRD cccccccchhhhhhhhhhcccccceeeccceeeecccchhhhhhhhhccccccccccccc

COILS

MEM

SEQ DAYSRMKLVLRWYLSGFYKKPKGIKKPYNPILGETFRCCWFHPQTDSRTFYIAEQVSHHP SEG PRD chhhhhhhhhhhhhhhcccccccccccccccccceeeeeecccccccceeeeeccccccc COILS

MEM

SEQ PVSAFHVSNRKDGFCISGSITAKSRFYGNSLSALLDGKATLTFLNRAEDYTLTMPYAHCK

SEG

PRD cceeeeecccccccccccccccccccccccccccccceeeeeeccccceeeeccccceee

COILS

MEM

SEQ GILYGTMTLELGGKVTIECAKNNFQAQLEFKLKPFFGGSTSINQISGKITSGEEVLASLS

SEG

PRD eeeeeccccccccceeeeeccccccceeeecccccccccccceeeeeccccccceeeeec

COILS

MEM

SEQ GHWDRDVFIKEEGSGSSALFWTPSGEVRRQRLRQHTVPLEEQTELESERLWQHVTRAISK

SEG

PRD cccccceeeeeccccceeeeeccccccccccccccccccchhhhhhhhhhhhhhhhhhhh

COILS

MEM

SEQ GDQHRATQEKFALEEAQRQRARERQESLMPWKPQLFHLDPITQEWHYRYEDHSPWDPLKD

SEG xxxxxxxxxxxxx

PRD cchhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccccccceeeeccccccccchh

COILS

MEM

SEQ IAQFEQDGILRTLQQEAVARQTTFLGSPGPRHERSGPDQRLRKASDQPSGHSQATESSGS

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhccccccccccccchhhhhcccccccccccccccccc

COILS

MEM

SEQ TPESCPELSDEEQDGDFVPGGEΞPCPRCRKEARRLQALHEAILSIREAQQELHRHLSAML

SEG

PRD ccccccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS

MEM

SEQ SSTARAAQAPTPGLLQSPRSWFLLCVFLACQLFINHILK

SEG

PRD hhhhhhhcccccccccccceeeeehhhhhhhhhhhhccc

COILS

MEM MMMMMMMMMMMMMMMMM .

Prosite for DKFZphutel_19hl7.3

PS00001 80->84 ASN_GLYCOSYLATION PDOC00001 PS00001 291->295 ASN_GLYCOSYLATION PDOC00001 PS00001 367->371 ASN_GLYCOSYLATION PDOC00001 PS00004 9->13 CAMP_PHOSPHO_SITE PDOC00004 PS00004 26->30 CAMP_PHOSPHO_SITE PDOC00004 PS00004 95->99 CAMP_PHOSPHO_ΞITE PDOC00004 PΞ00004 111->115 CAMP_PHOSPHO_SITE PDOC00004 PS00004 338->342 CAMP_PHOSPHO_SITE PDOC00004 PS00004 762->766 CAMP_PHOSPHO_SITE PDOC00004 PS00005 82->85 PKC_PHOSPHO_SITE PDOC00005 PS00005 90->93 PKC_PHOSPHO_SITE PDOC00005 PS00005 94->97 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 98->101 PKC_PHOSPHO_SITE PDOC00005 PS00005 132->135 PKC_PHOSPHO_SITE PDOC00005 PS00005 138->141 PKC_PHOSPHO_SITE PDOC00005 PS00005 159->162 PKC_PHOSPHO_SITE PDOC00005 PS00005 181->184 PKC_PHOSPHO_SITE PDOC00005 PS00005 252->255 PKC PHOSPHO SITE PDOC00005 1313 13131313 13131313131313131313 1313 U 13 13 U 1313 13131013 U 13131313131313131313 1313131313 ^rO 13131 r. 2 c W OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT CO OT CO OT OT CO OT OT CO CO OT OT OT CO

2 2 O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o σ o o o o O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O J A^j C> C0 ∞ CO ∞ ∞ C0 CO ∞ ∞ ∞ OT -J -J C O"ι CTl C^ CTl T> σt CJ^ σι Crι 3^ ^

DKFZphutel_19jll

group: uterus derived

DKFZphutel_19jll encodes a novel 708 amino acid protein with C-terminal similarity to several known proteins, such as human KIAA0231 or murine ras binding protein Sur8.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of uterus-specific genes .

Strong similarity to KIAA0231, similarity to ras binding protein Sur8

EST AA854189 extendes the sequence (294 Bp) , with this sequence complete cDNA,

Sequenced by AGOWA

Locus : unknown

Insert length: 2343 bp

Poly A stretch at pos. 2323, polyadenylation signal at pos. 2295

1 GCTCCTGCTA ACCCCATCAC TGTGGAAATG AAAGGCCTGA AGACAGATTT

51 GGACCTTCAG CAGTACAGCT TTATAAATCA GATGTGTTAT GAGCGAGCCC

101 TCCACTGGTA TGCCAAGTAT TTCCCTTACC TTGTCCTCAT CCATACCCTG

151 GTCTTTATGC TCTGCAGTAA CTTTTGGTTC AAATTCCCTG GTTCCAGCTC

201 CAAAATAGAA CATTTCATCT CCATTCTGGG GAAGTGTTTT GACTCTCCTT

251 GGACCACACG GGCTTTATCT GAAGTGTCTG GGGAGGACTC AGAAGAAAAG

301 GACAACAGGA AGAACAACAT GAACAGGTCC AACACCATCC AATCTGGTCC

351 AGAAGGCAGC CTGGTCAACT CTCAGTCTTT AAAGTCCATT CCTGAGAAGT

401 TTGTAGTTGA TAAATCCACT GCAGGGGCTC TGGATAAAAA GGAAGGTGAG

451 CAGGCTAAGG CCTTATTTGA GAAGGTGAAG AAGTTCAGGC TGCATGTGGA

501 AGAAGGTGAT ATTCTATATG CCATGTATGT TCGCCAGACT GTACTTAAAG

551 TTATCAAATT CCTAATCATC ATTGCATATA ATAGTGCTCT GGTTTCCAAG

601 GTCCAGTTTA CAGTGGACTG TAATGTGGAC ATTCAGGACA TGACTGGATA

651 TAAAAACTTT TCTTGCAATC ATACCATGGC ACACTTGTTC TCAAAACTGT

701 CCTTTTGCTA TCTGTGCTTT GTTAGTATCT ATGGATTGAC GTGCCTTTAT

751 ACCTTATACT GGCTGTTCTA CCGTTCTCTA CGGGAATATT CCTTTGAGTA

801 TGTCCGTCAG GAGACTGGAA TTGATGATAT TCCAGATGTG AAAAATGACT

851 TTGCTTTTAT GCTTCATATG ATAGATCAGT ATGACCCTCT CTATTCCAAG

901 AGATTTGCAG TGTTCCTGTC TGAAGTCAGT GAAAACAAAT TAAAGCAGCT

951 GAACTTAAAT AACGAATGGA CTCCTGATAA ACTGAGGCAG AAGCTACAGA

1001 CAAATGCCCA TAATCGACTG GAATTGCCTC TTATCATGCT CTCTGGCCTT

1051 CCAGACACTG TTTTTGAAAT CACAGAGTTG CAATCTCTAA AACTTGAAAT

1101 CATTAAGAAC GTAATGATAC CAGCCACCAT TGCACAGCTA GACAATCTTC

1151 AAGAGCTCTC TCTGCACCAG TGTTCTGTCA AAATCCACAG TGCGGCGCTC

1201 TCTTTCCTGA AGGAAAACCT CAAGGTCTTG AGCGTCAAGT TTGATGACAT

1251 GAGGGAACTC CCCCCCTGGA TGTATGGGCT CCGAAATCTG GAAGAGCTGT

1301 ACCTAGTTGG CTCTCTAAGT CATGATATTT CCAGAAATGT CACCCTTGAG

1351 TCTCTGCGGG ATCTCAAAAG CCTTAAAATT CTCTCTATCA AAAGCAACGT

1401 TTCCAAAATC CCTCAGGCAG TGGTTGATGT TTCCAGCCAT CTCCAGAAGA

1451 TGTGCATACA TAATGATGGC ACCAAGCTGG TGATGCTCAA CAACTTAAAG

1501 AAGATGACCA ATCTGACAGA GCTGGAGCTG GTCCACTGTG ACCTGGAGCG

1551 TATTCCTCAT GCTGTGTTCA GCCTACTCAG CCTCCAGGAA TTGGACCTGA

1601 AGGAAAACAA TCTGAAATCT ATAGAAGAAA TCGTTAGCTT TCAGCACTTA

1651 AGAAAGTTGA CAGTGCTAAA ACTGTGGCAT AACAGCATCA CCTACATCCC

1701 AGAGCATATA AAGAAACTCA CCAGCCTGGA ACGCCTGTCC TTTAGTCACA

1751 ATAAAATAGA GGTGCTGCCT TCCCACCTCT TCCTATGCAA CAAGATCCGA

1801 TACTTGGACT TATCGTACAA TGACATTCGA TTTATCCCCC CTGAAATTGG

1851 AGTTCTACAA AGTTTACAGT ATTTTTCCAT CACATGTAAC AAAGTGGAAA

1901 GCCTTCCAGA TGAACTCTAC TTCTGCAAGA AACTTAAAAC TCTGAAGATT

1951 GGAAAAAACA GCCTATCTGT ACTTTCACCG AAAATTGGAA ATTTGCTATT

2001 TCTTTCCTAC TTAGATGTAA AAGGTAATCA CTTTGAAATC CTCCCTCCTG

2051 AACTGGGTGA CTGTCGGGCT CTGAAGCGAG CTGGTTTAGT TGTAGAAGAT

2101 GCTCTGTTTG AAACTCTGCC TTCTGACGTC CGGGAGCAAA TGAAAACAGA

2151 ATAACTTATT TTTCGTTAAA GTTTGACTGA AACACGCTTC TACCAAATAC

2201 AGTATAAATA ATTAGGTAGT CTTAATGCCT TTCCTATTTT TTTTTCCTTT

2251 TCACACAAAA TGTACACAAA GATCGCGTAA GGAGTATGTA TTTTTAATAA

2301 AAATTTAATT GTATTTTTTC AATATTAAAA AAAAAAAAAA AAA

BLAST Results o BLAST result Medline entries

96421675:

Characterization of densin-180, a new brain-specific synaptie protein of the

O-sialoglycoprotem family.

98337190:

SUR-8, a conserved Ras-bmding protein with leucine-rich repeats, positively regulates Ras-mediated signaling in C. elegans .

Peptide information for frame 1

ORF from 28 bp to 2151 bp; peptide length: 708 Category: similarity to known protein Classification: Cell signaling/communication

1 MKGLKTDLDL QQYSFINQMC YERALHWYAK YFPYLVLIHT LVFMLCSNFW

51 FKFPGSΞSKI EHFISILGKC FDSPWTTRAL SEVSGEDSEE KDNRKNNMNR

101 SNTIQSGPEG SLVNSQSLKS IPEKFVVDKS TAGALDKKEG EQAKALFEKV

151 KKFRLHVEEG DILYAMYVRQ TVLKVIKFLI IIAYNSALVS KVQFTVDCNV

201 DIQDMTGYKN FSCNHTMAHL FSKLSFCYLC FVSIYGLTCL YTLYWLFYRS

251 LREYSFEYVR QETGIDDIPD VKNDFAFMLH MIDQYDPLYS KRFAVFLΞEV

301 SENKLKQLNL NNEWTPDKLR QKLQTNAHNR LELPLIMLSG LPDTVFEITE

351 LQSLKLEIIK NVMIPATIAQ LDNLQELSLH QCSVKIHSAA LSFLKENLKV

401 LSVKFDDMRE LPPWMYGLRN LEELYLVGSL SHDISRNVTL ESLRDLKSLK

451 ILSIKSNVSK IPQAVVDVSS HLQKMCIHND GTKLVMLNNL KKMTNLTELE

501 LVHCDLERIP HAVFSLLSLQ ELDLKENNLK SIEEIVSFQH LRKLTVLKLW

551 HNSITYIPEH IKKLTSLERL SFSHNKIEVL PSHLFLCNKI RYLDLSYNDI

601 RFIPPEIGVL QSLQYFSITC NKVESLPDEL YFCKKLKTLK IGKNSLSVLS

651 PKIGNLLFLS YLDVKGNHFE ILPPELGDCR ALKRAGLVVE DALFETLPSD

701 VREQMKTE

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_19j 11, frame 1

TREMBL :HSD984_1 gene: "KIAA0231"; Human mRNA for KIAA0231 gene, partial eds., N = 1, Score = 1408, P = 4.5e-144

TREMBL :AF054827_1 gene: "soc-2"; product: "leucine-rich repeat protein SOC-2"; Caenorhabditis elegans leucine-rich repeat protein SOC-2 (soc-2) mRNA, complete eds., N = 1, Score = 304, P = 5.7e-24

TREMBL :RNU66707_1 product: "densm-180"; Rattus norvegicus densm-180 mRNA, complete eds., N = 1, Score = 311, P = 7.4e-24

TREMBL :AF068921_1 product: "Ras-bmding protein SUR-8"; Mus musculus Ras-bmding protein SUR-8 mRNA, complete eds., N = 1, Score = 302, P = l.le-23

>TREMBL:HSD984_1 gene: "KIAA0231"; Human mRNA for KIAA0231 gene, partial eds .

Length = 476

HSPs:

Score = 1408 (211.3 bits), Expect = 4.5e-144, P = 4.5e-144 Identities = 265/471 (56%), Positives = 361/471 (76%)

Query: 237 LTCLYTLYWLFYRSLREYSFEYVRQETGIDDIPDVKNDFAFMLHMIDQYDPLYSKRFAVF 296

LT Y+L+W+ SL++YSFE +R+++ DIPDVKNDFAF+LH+ DQYDPLYSKRF++F Sbjct: 1 LTSSYSLWWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIF 60

Query: 297 LSEVSENKLKQLNLNNEWTPDKLRQKLQTNAHNRLELPLIMLSGLPDTVFEITELQSLKL 356

LSEVSENKLKQ+NLNNEWT +KL+ KL NA +++EL L ML+GLPD VFE+TE++ L L Sbjct: 61 LSEVSENKLKQINLNNEWTVEKLKSKLVKNAQDKIELHLFMLNGLPDNVFELTEMEVLSL 120 Query: 357 EIIKNVMIPATIAQLDNLQELSLHQCSVKIHSAALSFLKENLKVLSVKFDDMRELPPWMY 416

E+I V +P+ ++QL NL+EL ++ S+ + AL+FL+ENLK+L +KF +M ++P W++ Sb_jct: 121 ELIPEVKLPSAVSQLVNLKELRVYHSSLVVDHPALAFLEENLKILRLKFTEMGKIPRWVF 180

Query: 417 GLRNLEELYLVGSLSHDISRNVTLESLRDLKSLKILSIKSNVSKIPQAVVDVSSHLQKMC 476

L+NL+ELYL G + + + LE +DLK+L+ L +KS++S+IPQ V D+ LQK+ Sbjct: 181 HLKNLKELYLSGCVLPEQLSTMQLEGFQDLKNLRTLYLKSSLSRIPQVVTDLLPSLQKLS 240

Query: 477 IHNDGTKLVMLNNLKKMTNLTELELVHCDLERIPHAVFSLLSLQELDLKENNLKSIEEIV 536

+ N+G+KLV+LNNLKKM NL LEL+ CDLERIPH++FSL +L ELDL+ENNLK++EEI+ Sbjct: 241 LDNEGSKLVVLNNLKKMVNLKSLELISCDLERIPHSIFSLNNLHELDLRENNLKTVEEII 300

Query: 537 SFQHLRKLTVLKLWHNSITYIPEHIKKLTSLERLSFSHNKIEVLPΞHLFLCNKIRYLDLS 596

SFQHL+ L+ LKLWHN+I YIP I L++LE+LS HN IE LP LFLC K+ YLDLS Sbjct: 301 SFQHLQNLSCLKLWHNNIAYIPAQIGALSNLEQLSLDHNNIENLPLQLFLCTKLHYLDLS 360

Query: 597 YNDIRFIPPEIGVLQSLQYFSITCNKVESLPDELYFCKKLKTLKIGKNSLSVLSPKIGNL 656

YN + FIP El L +LQYF++T N +E LPD L+ CKKL+ L +GKNSL LSP +G L Sbjct: 361 YNHLTFIPEEIQYLSNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGKNSLMNLSPHVGEL 420

Query: 657 LFLSYLDVKGNHFEILPPELGDCRALKRAGLVVEDALFETLPSDVREQMKT 707

L++L++ GN+ E LPPEL C++LKR L+VE+ L TLP V E+++T Sbjct: 421 ΞNLTHLELIGNYLETLPPELEGCQSLKRNCLIVEENLLNTLPLPVTERLQT 471

Pedant information for DKFZphutel_19jll, frame 1

Report for DKFZphutel_19j 11.1

[LENGTH] 708

[MW] 81812.82

[pi] 7.55

[HOMOL] TREMBL:HSD984_1 gene: "KIAA0231"; Human mRNA for KIAA0231 gene, partial eds. le-149

[FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YJL005w] 3e-17

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YJL005w] 3e-17

[FUNCAT] 10.04.03 second messenger formation [S. cerevisiae, YJL005w] 3e-17

[FUNCAT] 01.03.10 metabolism of cyclic and unusual nucleotides [Ξ. cerevisiae,

YJL005w] 3e-17

[ FUNCAT ] 03.10 sporulation and germination [S. cerevisiae, YJL005w] 3e-17

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKL193c] 3e-09

[FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YKL193c] 3e-09

[FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YAL021c] 9e-08

[FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YAL021c]

9e-08

[ FUNCAT ] 01.01.04 regulation of amino-acid metabolism [S. cerevisiae, YAL021c]

9e-08

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YOR353c] 3e-07

[BLOCKS] BL00868F

[BLOCKS] BL00985B Spermadhesins family proteins

[EC] 3.4.17.3 Lysine carboxypeptidase le-08

[EC] 4.6.1.1 Adenylate cyclase 3e-18

[PIRKW] blocked amino end le-10

[PIRKW] phosphotransferase le-09

[PIRKW] nucleus 6e-08

[PIRKW] duplication 3e-18

[PIRKW] platelet le-10

[PIRKW] tandem repeat 7e-16

[PIRKW] keratan sulfate 7e-07

[PIRKW] metallo-carboxypeptidase le-08

[PIRKW] transmembrane protein le-10

[PIRKW] serme/threonine-specific protein kinase le-09

[PIRKW] autophosphorylation le-09

[PIRKW] cartilage 7e-07

[PIRKW] connective tissue 7e-07

[PIRKW] magnesium le-09

[PIRKW] cAMP biosynthesis 3e-18

[PIRKW] ATP le-09

[PIRKW] receptor le-09

[PIRKW] leucine zipper 3e-13

[PIRKW] glycoprotein 5e-12

[PIRKW] extracellular matrix 7e-07

[PIRKW] chondro tin sulfate proteoglycan 7e-07

[PIRKW] cell adhesion le-08

[PIRKW] hydrolase le-08

[PIRKW] sulfoprotein 7e-07

[PIRKW] membrane protein le-08

[PIRKW] phosphorus-oxygen lyase 3e-18 [PIRKW] collagen binding 7e-07

[SUPFAM] leucine-rich alpha-2-glycoprotem repeat homology 3e-21

[SUPFAM] chaoptm le-08

[SUPFAM] gelsolm repeat homology 3e-21

[SUPFAM] protein kinase homology le-09

[SUPFAM] protein kinase Xa21 le-09

[SUPFAM] fibromodulin 4e-12

[SUPFAM] yeast adenylate cyclase catalytic domain homology 3e-18

[SUPFAM] yeast adenylate cyclase 3e-18

[KW] TRANSMEMBRANE 3

[KW] LOW_COMPLEXITY 1.41 %

SEQ MKGLKTDLDLQQYSFINQMCYERALHWYAKYFPYLVLIHTLVFMLCSNFWFKFPGSSSKI SEG PRD ccccchhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhhccceeeeccccccee MEM MMMMMMMMMMMMMMMMM

SEQ EHFISILGKCFDSPWTTRALSEVSGEDSEEKDNRKNNMNRSNTIQΞGPEGSLVNSQSLKS SEG PRD eeeeeeeecccccccceeeeecccccccccccccccccccccccccccccceeeeccccc

MEM

SEQ IPEKFVVDKSTAGALDKKEGEQAKALFEKVKKFRLHVEEGDILYAMYVRQTVLKVIKFLI SEG PRD cccceeecccccccccchhhhhhhhhhhhhhhhhhhhcccceeeehhhhhhhhhhhhhhh MEM MMMMMMMMM

SEQ IIAYNSALVSKVQFTVDCNVDIQDMTGYKNFSCNHTMAHLFSKLSFCYLCFVSIYGLTCL SEG PRD hhhhcchhhhheeeeeccccccccccccccccccchhhhhhhhheeeeeeeeeeccceee MEM MMMMMMMM MMMMMMMMMMMMMMMMM

SEQ YTLYWLFYRSLREYΞFEYVRQETGIDDIPDVKNDFAFMLHMIDQYDPLYSKRFAVFLSEV SEG PRD hhhhhhhhhhhhhhhhhhhhhhccccccccccchhhhhhhhhhcccchhhhhhhhhhhhh

MEM

SEQ SENKLKQLNLNNEWTPDKLRQKLQTNAHNRLELPLIMLSGLPDTVFEITELQSLKLEIIK SEG .. xxxxxxxxxx PRD hhhhhhhhhccccccccchhhhhhhhhhhhhhhhhhhcccccccchhhhhhhhhhhhhhh

MEM

SEQ NVMIPATIAQLDNLQELSLHQCSVKIHSAALSFLKENLKVLSVKFDDMRELPPWMYGLRN SEG PRD hccccccchhhhhhhhhhhhccccccccccccchhhhhhhhhhccccccccccccchhhh MEM

SEQ LEELYLVGSLSHDIΞRNVTLESLRDLKSLKILSIKSNVSKIPQAVVDVSSHLQKMCIHND SEG PRD hhhhhhccccccccccccccchhhhhhhhhhhhcccccccccccchhhhhhhhhhhcccc MEM

SEQ GTKLVMLNNLKKMTNLTELELVHCDLERIPHAVFSLLSLQELDLKENNLKSIEEIVSFQH

SEG PRD ceeeecccccccchhhhhhhhhccccccccccchhhhhhhhhhhccccccccccccccch MEM

SEQ LRKLTVLKLWHNSITYIPEHIKKLTSLERLSFSHNKIEVLPSHLFLCNKIRYLDLSYNDI SEG PRD hhhhhhhcccccceeecccccchhhhhheeeccccceeecccccchhhhhhhhhhccccc MEM

SEQ RFIPPEIGVLQSLQYFSITCNKVESLPDELYFCKKLKTLKIGKNSLSVLSPKIGNLLFLS SEG PRD cccccccchhhhhhhhhhhccccccccccccchhhhhcccccccceeecccccccchhhh MEM

SEQ YLDVKGNHFEILPPELGDCRALKRAGLVVEDALFETLPSDVREQMKTE SEG PRD hhhccccccccccccchhhhhhhhheeeeccccccccccccccccccc MEM

(No Prosite data available for DKFZphutel_19j 11.1) (No Pfam data available for DKFZphutel_19j 11.1) DKFZphutel_lι2

group: transcription factor

DKFZphutel_lι2 encodes a novel 594 amino acid protein similar to signal transducing proteins.

The protein contains 2 WD-40 repeats, which is typical for the beta-transducin subunit of G- proteins . In addition, the protein contains a C3HC4 zinc finger and a leucine zipper. The beta subunits seem to be required for the replacement of GDP by GTP as well as for membrane anchoring and receptor recognition. Due to the zmc finger the novel protein seems to be a new molecule involved in signal transduction and transcription.

The new protein can find application in modulating/blocking gene expression of genes controlled by this molecule. similarity to Dictostelium myosin heavy chain kinase complete cDNA, complete eds, EST hits

[PFAM] Zinc finger, C3HC4 type (RING fmger)

[PFAM] WD domain, G-beta repeats

[SCOP] dltbgc_ 2.46.3.1.1 betal-subumt of the signal-transducing G protei 3e-07

Sequenced by BMFZ

Locus: /map="16pl3.3"

Insert length: 3584 bp

Poly A stretch at pos. 3555, polyadenylation signal at pos. 3537

1 GGGCGGGAGG TGCTTCCCAA GGACCGTAGA TGCCTCTCTA GAGCATGAGC 51 TCAGGCAAGA GTGCCCGCTA CAACCGCTTC TCCGGGGGGC CCAGCAATCT

101 TCCCACCCCA GACGTCACCA CAGGGACCAG AATGGAAACG ACCTTCGGAC

151 CCGCCTTTTC AGCCGTCACC ACCATCACAA AAGCTGACGG GACCAGCACC

201 TACAAGCAGC ACTGCAGGAC AGCATGCCCC CCATCAGCAC TCCCCGCCGC

251 TCCGACTCCG CCATCTCTGT CCGCTCCCTG CACTCAGAGT CCAGCATGTC

301 TCTGCGCTCC ACATTCTCAC TGCCCGAGGA GGAGGAGGAG CCGGAGCCAC

351 TGGTGTTTGC GGAGCAGCCC TCGGTGAAGC TGTGCTGTCA GCTCTGCTGC

401 AGCGTCTTCA AAGACCCCGT GATCACCACG TGTGGGCACA CGTTCTGTAG

451 GAGATGCGCC TTGAAGTCAG AGAAGTGTCC CGTGGACAAC GTCAAACTGA

501 CCGTGGTGGT GAACAACATC GCGGTGGCCG AGCAGATCGG GGAGCTCTTC

551 ATCCACTGCC GGCACGGCTG CCGGGTAGCG GGCAGCGGGA AGCCCCCCAT

601 CTTTGAGGTG GACCCCCGAG GGTGCCCCTT CACCATCAAG CTCAGCGCCC

651 GGAAGGACCA CGAGGGCAGC TGTGACTACA GGCCTGTGCG GTGTCCCAAC

701 AACCCCAGCT GCCCCCCGCT GCTCAGGATG AACCTGGAGG CCCACCTCAA

751 GGAGTGCGAG CACATCAAAT GCCCCCACTC CAAGTACGGG TGCACGTTCA

801 TCGGGAACCA GGACACTTAC GAGACCCACC TGGAGACTTG CCGCTTCGAG

851 GGCCTGAAGG AGTTTCTGCA GCAGACGGAT GACCGCTTCC ACGAGATGCA

901 CGTGGCTCTG GCCCAGAAGG ACCAGGAGAT CGCCTTCCTG CGCTCCATGC

951 TGGGAAAGCT CTCGGAGAAG ATCGACCAGC TAGAGAAGAG CCTGGAGCTC 1001 AAGTTTGACG TCCTGGACGA AAACCAGAGC AAGCTCAGCG AGGACCTCAT 1051 GGAGTTCCGG CGGGACGCAT CCATGTTAAA TGACGAGCTG TCCCACATCA 1101 ACGCGCGGCT GAACATGGGC ATCCTAGGCT CCTACGACCC TCAGCAGATC 1151 TTCAAGTGCA AAGGGACCTT TGTGGGCCAC CAGGGCCCTG TGTGGTGTCT 1201 CTGCGTCTAC TCCATGGGTG ACCTGCTCTT CAGTGGCTCC TCTGACAAGA 1251 CCATCAAGGT GTGGGACACA TGTACCACCT ACAAGTGTCA GAAGACACTG 1301 GAGGGCCATG ATGGCATCGT GCTGGCTCTC TGCATCCAGG GGTGCAAACT 1351 CTACAGCGGC TCTGCAGACT GCACCATCAT TGTGTGGGAC ATCCAGAACC 1401 TGCAGAAGGT GAACACCATC CGGGCCCATG ACAACCCGGT GTGCACGCTG 1451 GTCTCCTCAC ACAACGTGCT CTTCAGCGGC TCCCTGAAGG CCATCAAGGT 1501 CTGGGACATC GTGGGCACTG AGCTGAAGTT GAAGAAGGAG CTCACAGGCC 1551 TCAACCACTG GGTGCGGGCC CTGGTGGCTG CCCAGAGCTA CCTGTACAGC 1601 GGCTCCTACC AGACAATCAA GATCTGGGAC ATCCGAACCC TTGACTGCAT 1651 CCACGTCCTG CAGACGTCTG GTGGCAGCGT CTACTCCATT GCTGTGACAA 1701 ATCACCACAT TGTCTGTGGC ACCTACGAGA ACCTCATCCA CGTGTGGGAC 1751 ATTGAGTCCA AGGAGCAGGT GCGGACCCTC ACGGGCCACG TGGGCACCGT 1801 GTATGCCCTG GCGGTCATCT CGACGCCAGA CCAGACCAAA GTCTTCAGTG 1851 CATCCTACGA CCGGTCCCTC AGGGTCTGGA GTATGGACAA CATGATCTGC 1901 ACGCAGACCC TGCTGCGTCA CCAGGGCAGT GTCACCGCGC TGGCTGTGTC 1951 CCGGGGCCGA CTCTTCTCAG GGGCTGTGGA TAGCACTGTG AAGGTTTGGA 2001 CTTGCTAACA GGATCCAGGC CAGGCTGTGG TTTCCCCTGA ACCAGCCCTG 2051 GACCTTTCTG AGCCAGGCTG GCCACATGGG GTGGTCTCGG GGTTTCTGCC 2101 TGCCCCGTGG GCATAGGTGG ACAGGCTCTG GCAGCCGGGC AGTGCCCTCC 2151 CCGTCCCATG CTCGGCGAGC CTCCCTCTAC TCGGCACTGT CCTTGCTGCC 2201 CAGCCCCTCT CTGGGTGCCA GGTACGACGC TTGCCCCGGC CCACCCTCCA 2251 TCCCCACCCT CCATCCCCAC CCTAGATGGA GCGAGGGCCT TTTTACTCAC 2301 CTTTTCTACC GTTTTTAGAC TGTATGTAGA TTTGGTTACC TCCTGGTTGA 2351 AATAAATGCT CCACAGACTG TGGCTGTGAG TGGGGACAGC TCCTCGGGAC 2401 AAGGGGGCTG TGTGTGGCCT TGAGGTTGGT GTGCACAGGC ACTGGCTGCT 2451 GTGAGTGGGG GGGCATGGGG CAGTTTCCTT TGGTGGACCC CAGGACTTCG 2501 GCCCACTCCG GGGCCTCCCC TCCCTGCTAG GAGGCAACTC GTCACACCCA 2551 AGCTGCTGGC CTCCAGTCCC ATCTCCCCCA ACACATGTGC CCCCAAAAAG 2601 TGAGCCAGGC ACCTCTGTTT CCTGCTGTTT ATTGACAGCC GACGGCAGCG 2651 CCTTGCCCAG ACCTCCCCTG CCCACCTGCT GGAGCCCAGC CTGTGCCGCC 2701 CTCTGAGGAG AGGCCTGGGG GGACAGCTGG GCACGTCCAC TCGCAGGGAA ₂751 ACACGGGGTG AGACAGCAGG AAGGGGCCCT GCACGCCGGG ACGCCACCTC 2801 CGCCAGCCGC CTCCACCCGC CCCACACCAC AATCGCTGGT TTTCGGCATT 2851 TTTTAAATTT TTTTTTTAAG AAACGTCAAA GTTGTGCCCA ACACTGTGGA 2901 TCAGCAAACA CGATAGAGGA GACCAGTCAG TACTTCTTGG AGGGGGCAGG 2951 AGGAGAGAGG AAAAGGGAGG GCGAGAATGA CCACACAACA CAGCCTTGGA 3001 CCATGAGCAG AAGCGTCCGT GGGAACTCCA CTGGGGTGGA TGGGCTGCCT 3051 GCACAGCCCC TGGAGAGGGG GCCAGGCACA CCCTCAGAGG AGCTGCAAGC 3101 CCGTGGCCTG GCCTGCTACA TGCCCTGCTT CCACGTGGCT GCCACGCTGA 3151 CACACCCACA TTCACCAAAC CCACCCGCGC CCTGGGACGC AGCCACGCCA 3201 GGAGGAGGAC ACGGCCGCCG AGAGCAAGGC ACAACCTCGA GTTCTTGGGG 3251 CGCAGAGAAC TTAGGAGAGA AGCACGGAGG AGCCCCCGGC AGAGCACCCG 3301 CCCCCGGGCC CCAGCCTTCC ACCTGTGCTA GCAGCCTGGG GCCTCCACTC 3351 TGGCCGGAGG AAGGACCGCA GGCAGACAGC CTGGGCCTCT AACAGCTTTT 3401 GTCCGGAGCT AGACTTCGTG TCCTTTCAGT TGGTAAATGG TTTTCTATAG 3451 AATCAATAAT ATTTCTTTCT TTAAATATAT ATTTGTTAAA GTTATACCTT 3501 TTTGTTTCTC TGGGGAAATC CGCCTCAGCT CATTCCCAAT AAATTAATAC 3551 TCTTGATAAA AAAAAAAAAA AGAAAAAAAA AAAA

BLAST Results

Entry HSBE from database EMBL:

Homo sapiens (clone exon trap d5) chromosome 16pl3.3 gene, exon.

Score = 2375, P = 7. le-101, identities = 475/475

Entry HSBD from database EMBL:

Homo sapiens (clone exon trap d32) chromosome 16pl3.3 gene, exon.

Score = 876, P = 3.0e-31, identities = 176/177

Medl e entries

95122486:

Structural analysis of myosin heavy chain kinase A from

Dictyostelium. Evidence for a highly divergent protein kinase domain, an ammo-terminal coiled-coil domain, and a domain homologous to the beta-subumt of heterotπmeric G proteins .

96149460:

Dictyostelium myosin heavy chain kinase A regulates myosin localization during growth and development.

97277316:

Identification of a protein kinase from Dictyostelium with homology to the novel catalytic domain of myosin heavy chain kinase A.

96009891:

A gene responsible for vegetative incompatibility in the fungus

Podospora anser a encodes a protein with a GTP-bindmg motif and G beta homologous domain.

Peptide information for frame 2

ORF from 224 bp to 2005 bp; peptide length: 594

Category: similarity to known protein

Prosite motifs: ZINC_FINGER_C3HC4 (70-80)

LEUCINE_ZIPPER (436-458)

LEUCINE_ZIPPER (436-458)

G_BETA_REPEATS (335-355)

G BETA REPEATS (376-391) 1 MPPISTPRRS DSAISVRSLH ΞESSMSLRST FSLPEEEEEP EPLVFAEQPS

51 VKLCCQLCCS VFKDPVITTC GHTFCRRCAL KSEKCPVDNV KLTVVVNNIA

101 VAEQIGELFI HCRHGCRVAG SGKPPIFEVD PRGCPFTIKL SARKDHEGSC

151 DYRPVRCPNN PSCPPLLRMN LEAHLKECEH IKCPHSKYGC TFIGNQDTYE

201 THLETCRFEG LKEFLQQTDD RFHEMHVALA QKDQEIAFLR SMLGKLSEKI

251 DQLEKSLELK FDVLDENQSK LSEDLMEFRR DASMLNDELS HINARLNMGI

301 LGSYDPQQIF KCKGTFVGHQ GPVWCLCVYS MGDLLFSGSS DKTIKVWDTC

351 TTYKCQKTLE GHDGIVLALC IQGCKLYSGS ADCTIIVWDI QNLQKVNTIR

401 AHDNPVCTLV SSHNVLFSGS LKAIKVWDIV GTELKLKKEL TGLNHWVRAL

451 VAAQSYLYSG SYQTIKIWDI RTLDCIHVLQ TSGGSVYSIA VTNHHIVCGT

501 YENLIHVWDI ESKEQVRTLT GHVGTVYALA VISTPDQTKV FSASYDRSLR

551 VWSMDNMICT QTLLRHQGSV TALAVSRGRL FSGAVDSTVK VWTC

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_lι2, frame 2

SWISSPROT :KMHB_DICDI MYOSIN HEAVY CHAIN KINASE B (EC 2.7.1.129) (MHCK B)., N = 1, Score = 419, P = 3.6e-37

SWISSPROT :HETl_PODAN VEGETATIBLE INCOMPATIBILITY PROTEIN HET-E-1., N = 1, Score = 392, P = 3.1e-33

SWISSPROT :YDJ5_SCHPO HYPOTHETICAL 67.1 KD TRP-ASP REPEATS CONTAINING PROTEIN C57A10.05C IN CHROMOSOME I., N = 1, Score = 357, P = 4.1e-30

TREMBL:AF032878_1 gene: "slimb"; product: "Slimb"; Drosophila melanogaster Slimb (slimb) mRNA, complete eds., N = 1, Score = 347, P = 1.7e-29

>SWISΞPROT:KMHB_DICDI MYOSIN HEAVY CHAIN KINASE B (EC 2.7.1.129) (MHCK B) . Length = 732

HSPs:

Score = 419 (62.9 bits), Expect = 3.6e-37, P = 3.6e-37 Identities = 96/268 (35%), Positives = 158/268 (58%)

Query: 325 CLCVYSMGDLLFSGSSDKTIKVWD-TCTTYKCQKTLEGHDGIVLALCIQGCKLYSGSADC 383

C+C +LLF+G SD +I+V+D +C +TL+GH+G V ++C L+SGS+D Sbjct: 467 CIC DNLLFTGCSDNSIRVYDYKSQNMECVQTLKGHEGPVESICYNDQYLFSGSSDH 522

Query: 384 TIIVWDIQNLQKVNTIRAHDNPVCTLVSSHNVLFSGSL-KAIKVWDIVGTELKLKKELTG 442

+1 VWD++ L+ + T+ HD PV T++ + LFSGS K IKVWD+ L+ K L Sbjct: 523 SIKVWDLKKLRCIFTLEGHDKPVHTVLLNDKYLFSGSSDKTIKVWDL—KTLECKYTLES 580

Query: 443 LNHWVRALVAAQSYLYSGSY-QTIKIWDIRTLDCIHVLQTΞGGSVYSIAVTNHHIVCGTY 501

V+ L + YL+SGS +TIK+WD++T C + L+ V +1 + ++ G+Y Sbjct: 581 HARAVKTLCISGQYLFSGSNDKTIKVWDLKTFRCNYTLKGHTKWVTTICILGTNLYSGSY 640

Query: 502 ENLIHVWDIESKEQVRTLTGHVGTVYALAVISTPDQTKVFSASYDRSLRVWSMDNMICTQ 561

+ I VW+++S E TL GH V + + D+ +F+AS D ++++W ++ + C Sbjct: 641 DKTIRVWNLKSLECSATLRGHDRWVEHMVIC DKL-LFTASDDNTIKIWDLETLRCNT 696

Query: 562 TLLRHQGSVTALAVSRGR—LFSGAVDSTVKVW 592

TL H +V LAV + + S + D +++VW Sbjct: 697 TLEGHNATVQCLAVWEDKKCVISCSHDQSIRVW 729

Score = 415 (62.3 bits). Expect = 1.2e-36, P = 1.2e-36 Identities = 113/303 (37%), Positives = 166/303 (54%)

Query: 255 KSLEL-KFDVLDENQSKLSEDLMEFRRDASMLNDEL-SHINARLNMGILGS YD 305

KS++L K ++L N+ K S +L + ++ + SH+ N+ G YD Sbjct: 427 KSIDLEKPEILINNKKKESINLETIKLIETIKGYHVTSHLCICDNLLFTGCSDNSIRVYD 486

Query: 306 -PQQIFKCKGTFVGHQGPVWCLCVYSMGDLLFSGSΞDKTIKVWDTCTTYKCQKTLEGHDG 364

Q +C T GH+GPV +C Y+ LFSGSSD +IKVWD +C TLEGHD Sbjct: 487 YKSQNMECVQTLKGHEGPVESIC-YN-DQYLFSGSSDHSIKVWDL-KKLRCIFTLEGHDK 543

Query: 365 IVLALCIQGCKLYSGSADCTIIVWDIQNLQKVNTIRAHDNPVCTLVSSHNVLFSGSL-KA 423

V + + L+SGS+D TI VWD++ L+ T+ +H V TL S LFSGS K Sbjct: 544 PVHTVLLNDKYLFSGSSDKTIKVWDLKTLECKYTLESHARAVKTLCISGQYLFSGSNDKT 603

Query: 424 IKVWDIVGTELKLKKELTGLNHWVRALVAAQSYLYSGSY-QTIKIWDIRTLDCIHVLQTS 482

IKVWD+ + L G WV + + LYSGSY +TI++W++++L+C L+ Sbjct: 604 IKVWDL—KTFRCNYTLKGHTKWVTTICILGTNLYSGSYDKTIRVWNLKSLECSATLRGH 661 Query: 483 GGSVYSIAVTNHHIVCGTYENLIHVWDIESKEQVRTLTGHVGTVYALAVISTPDQTKVFS 542

V + + + + + +N I +WD+E+ TL GH TV LAV D+ V S

Sbjct: 662 DRWVEHMVICDKLLFTASDDNTIKIWDLETLRCNTTLEGHNATVQCLAVWE—DKKCVIS 719

Query: 543 ASYDRSLRVW 552

S+D+S+RVW

Sbjct: 720 CSHDQSIRVW 729

Score = 262 (39.3 bits), Expect = 3.2e-19, P = 3.2e-19 Identities == 60/184 (32%), Positives = 109/184 (59%)

Query: 352 TYKCQKTLEGHDGIVLALCIQGCKLYSGSADCTIIVWDI--QNLQKVNTIRAHDNPVCTL 409 T K +T++G+ + LCI L++G +D +1 V+D QN++ V T++ H+ PV ++ Sbjct: 450 TIKLIETIKGYH-VTSHLCICDNLLFTGCSDNSIRVYDYKSQNMECVQTLKGHEGPVESI 508 Query: 410 VSSHNVLFSGSLK-AIKVWDIVGTELKLKKELTGLNHWVRALVAAQSYLYSGSY-QTIKI 467

+ LFSGS +IKVWD+ +L+ L G + V ++ YL+SGS +TIK+ Sbjct: 509 CYNDQYLFSGSSDHSIKVWDL--KKLRCIFTLEGHDKPVHTVLLNDKYLFSGSSDKTIKV 566 Query: 468 WDIRTLDCIHVLQTSGGSVYSIAVTNHHIVCGTYENLIHVWDIESKEQVRTLTGHVGTVY 527 WD++TL+C + L++ +V ++ ++ ++ G+ + I VWD+++ TL GH V Sbjct: 567 WDLKTLECKYTLESHARAVKTLCIΞGQYLFSGSNDKTIKVWDLKTFRCNYTLKGHTKWVT 626 Query: 528 ALAVIST 534

+ ++ T Sbjct: 627 TICILGT 633

Score ^: = 173 (26.0 bits), Expect = 1.7e-09, P = 1.7e-09

Identities ^; . 43/118 (36%), Positives = 65/118 (55%)

Query: 310 FKCKGTFVGHQGPVWCLCVYSMGDLLFSGSSDKTIKVWDTCTTYKCQKTLEGHDGIVLAL 369 F+C T GH V +C+ +G L+SGS DKTI+VW+ + +C TL GHD V +

Sbjct: 612 FRCNYTLKGHTKWVTTICI—LGTNLYSGSYDKTIRVWNL-KSLECSATLRGHDRWVEHM 668

Query: 370 CIQGCKLYSGSADCTIIVWDIQNLQKVNTIRAHDNPV-CTLVSSH —VLFSGSLKAIKV 426

I L++ S D TI +WD++ L+ T+ H+ V C V V+ ++I+V

Sbjct: 669 VICDKLLFTASDDNTIKIWDLETLRCNTTLEGHNATVQCLAVWEDKKCVISCSHDQSIRV 728

Query: 427 W 427

W

Sbjct: 729 W 729

Pedant information for DKFZphutel_lι2, frame 2

Report for DKFZphutel_lι2.2

[LENGTH] 594 [MW] 66541.94 [pi] 6.64 [HOMOL] SWISSPROT :KMHB DICDI MYOSIN HEAVY CHAIN KINASE B (EC 2.7.1.129) (MHCK B) . 3e-37

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YIL046w] 5e-21

[FUNCAT] 06.13.01 cytoplasmic degradation [S. cerevisiae, YIL046w] 5e-21

[FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YIL046w] 5e-21

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YIL046w] 5e-21

[FUNCAT] 01.01.04 regulation of amino-acid metabolism [S. cerevisiae, YIL046w]

5e-21

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YCR072c beta-transducm family]

2e-15

[FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YFL009w] le-14

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YFL009w] le-14

[FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YFL009w] le-14

[FUNCAT] 03.16 dna synthesis and replication [Ξ. cerevisiae, YFL009w] le-14

[FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae,

YDL145C] le-13

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL145c] le-13

[FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YPR178w] 2e-ll

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YPR178w] 2e-ll

[FUNCAT] 04.05.01.01 general transcription activities [S. cerevisiae, YBR198c

TAF90 - TFIID subunit] 3e-ll

[FUNCAT] 03.13 meiosis [S. cerevisiae, YLR129w] 8e-09

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YCR057c] 2e-07

[FUNCAT] 03.25 cytokinesis [S. cerevisiae, YCR057c] 2e-07

[FUNCAT] 02.16 fermentation [S. cerevisiae, YMR116c] 5e-07

[FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae,

YMR116C] 5e-07 [FUNCAT] 06.13 proteolysis [S. cerevisiae, YGL003c] 3e-06 [FUNCAT] 03.01 cell growth [S. cerevisiae, YKL021c] 2e-04 [FUNCAT] 01.03.07 deoxyribonucleotide metabolism [S. cerevisiae, YOR269w] 2e-04 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YOR212w] 0.001 [ FUNCAT ] 10.05.07 g-proteins [S. cerevisiae, YOR212w) 0.001 [FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins

[S. cerevisiae, YOR212w] 0.001 [BLOCKS] BL00678 [BLOCKS] BL00518 Zinc finger, C3HC4 type, proteins [SCOP] dltbgd_ 2.46.3.1.1 betal-subunit of the signal-transducmg 3e-10 [EC] 2.7.1.129 Myosin-heavy-cham kinase 3e-26 [PIRKW] phosphotransferase 3e-26 [PIRKW] nucleus le-06 [PIRKW] plasma 9e-08 [PIRKW] duplication 3e-25 [PIRKW] hormone 9e-08 [PIRKW] zinc 3e-09 [PIRKW] cell cycle control 4e-13 [PIRKW] transmembrane protein 3e-12 [PIRKW] zinc finger le-08 [PIRKW] stomach 9e-08 [PIRKW] DNA binding 9e-06 [PIRKW] autophosphorylation 3e-26 [PIRKW] phosphoprotem 3e-26 [PIRKW] signal transduction 5e-08 [PIRKW] heterotrimer 5e-08 [PIRKW] coiled coil 3e-26 [PIRKW] multimer 3e-26 [PIRKW] transcription regulation 4e-10 [PIRKW] GTP binding 5e-08 [SUPFAM] chromobox homology 9e-06 [SUPFAM] RING finger homology 3e-09 [SUPFAM] coatomer complex beta' chain le-07 [SUPFAM] WD repeat homology 3e-26 [SUPFAM] yeast coatomer complex alpha chain 3e-12 [SUPFAM] GTP-bindmg regulatory protein beta chain 5e-08 [SUPFAM] PRL1 protein 2e-09 [PROSITE] WD_REPEA S 2 [PROSITE] LEUCINE_ZIPPER 1 [PROSITE] MYRISTYL 14 [PROSITE] CK2_PHOSPHO_SITE 4 [PROSITE] ZINC_FINGER_C3HC4 1 [PROSITE] PKC_PHOSPHO_SITE 18 [PROSITE] ASN_GLYCOSYLATION 1 [PFAM] Zmc finger, C3HC4 type (RING finger) [PFAM] WD domain, G-beta repeats [KW] Irregular [KW] 3D [KW] LOW_COMPLEXITY 6.23 % [KW] COILED COIL 6.73 %

SEQ MPPISTPRRSDSAISVRSLHSESSMSLRSTFSLPEEEEEPEPLVFAEQPSVKLCCQLCCS SEG xxxxxxxxxxxxxx .... xxxxxxxxx COILS lgg2B

SEQ VFKDPVITTCGHTFCRRCALKSEKCPVDNVKLTVVVNNIAVAEQIGELFIHCRHGCRVAG SEG COILS lgg2B

SEQ SGKPPIFEVDPRGCPFTIKLSARKDHEGSCDYRPVRCPNNPSCPPLLRMNLEAHLKECEH SEG COILS lgg2B

SEQ IKCPHSKYGCTFIGNQDTYETHLETCRFEGLKEFLQQTDDRFHEMHVALAQKDQEIAFLR SEG COILS cccccccccccccc lgg2B

SEQ SMLGKLSEKIDQLEKSLELKFDVLDENQSKLSEDLMEFRRDASMLNDELSHINARLNMGI SEG COILS cccccccccccccccccccccccccc lgg2B

SEQ LGSYDPQQIFKCKGTFVGHQGPVWCLCVYSMGDLLFSGSSDKTIKVWDTCTTYKCQKTLE SEG COILS lgg2B . EECCCCCCEEEEEETTTTCEEEEEETTTEEEEEEG-GGCEEEEEEE SEQ GHDGIVLALCIQGCKLYSGSADCTIIVWDIQNLQKVNTIRAHDNPVCTLVSSHNVLFSGS SEG COILS lgg2B CCCCCEEEEEETTCEEEEEETTTCEEEEETTTTEEEEEE-CTTTTCCCEEE.

SEQ LKAIKVWDIVGTELKLKKELTGLNHWVRALVAAQSYLYSGSYQTIKIWDIRTLDCIHVLQ SEG xxxxxxxxxxxxx COILS lgg2B

SEQ TSGGSVYSIAVTNHHIVCGTYENLIHVWDIESKEQVRTLTGHVGTVYALAVISTPDQTKV SEG COILS lgg2B

SEQ FSASYDRSLRVWSMDNMICTQTLLRHQGSVTALAVSRGRLFSGAVDSTVKVWTC SEG COILS lgg2B

Prosite for DKFZphutel_lι2.2

PS00001 267->271 ASN_GLYCOSYLATION PDOC00001 PS00005 6->9 PKC_PHOSPHO_SITE PDOC00005 PS00005 15->18 PKC_PHOSPHO_SITE PDOC00005 PS00005 26->29 PKC_PHOSPHO_SITE PDOC00005 PS00005 50->53 PKC_PHOSPHO_SITE PDOC00005 PS00005 82->85 PKC_PHOSPHO_SITE PDOC00005 PS00005 121->124 PKC_PHOSPHO_SITE PDOC00005 PS00005 137->140 PKC_PHOΞPHO_SITE PDOC00005 PS00005 141->144 PKC_PHOSPHO_SITE PDOC00005 PS00005 205->208 PKC_PHOSPHO_SITE PDOC00005 PS00005 247->250 PKC_PHOSPHO_SITE PDOC00005 PS00005 340->343 PKC_PHOSPHO_SITE PDOC00005 PS00005 343->346 PKC_PHOSPHO_SITE PDOC00005 PS00005 352->355 PKC_PHOSPHO_SITE PDOC00005 PS00005 398->401 PKC_PHOSPHO_SITE PDOC00005 PS00005 420->423 PKC_PHOSPHO_SITE PDOC00005 PS00005 464->467 PKC_PHOSPHO_SITE PDOC00005 PS00005 548->551 PKC_PHOSPHO_SITE PDOC00005 PS00005 588->591 PKC_PHOSPHO_SITE PDOC00005 PS00006 32->36 CK2_PHOSPHO_SITE PDOC00006 PS00006 201->205 CK2_PHOSPHO_SITE PDOC00006 PS00006 330->334 CK2_PHOSPHO_SITE PDOC00006 PS00006 533->537 CK2_PHOSPHO_SITE PDOC00006 PS00008 115->121 MYRISTYL PDOC00008 PS00008 133->139 MYRISTYL PDOC00008 PS00008 194->200 MYRISTYL PDOC00008 PS00008 299->305 MYRISTYL PDOC00008 PS00008 314->320 MYRISTYL PDOC00008 PS00008 364->370 MYRISTYL PDOC00008 PS00008 379->385 MYRISTYL PDOC00008 PS00008 419->425 MYRISTYL PDOC00008 PS00008 460->466 MYRISTYL PDOC00008 PS00008 484->490 MYRISTYL PDOC00008 PS00008 499->505 MYRISTYL PDOC00008 PS00008 524->530 MYRISTYL PDOC00008 PS00008 568->574 MYRISTYL PDOC00008 PS00008 583->589 MYRISTYL PDOC00008 PS00518 70->80 ZINC_FINGER_C3HC4 PDOC00449 PS00029 436->458 LEUCINE_ZIPPER PDOC00029 PS00678 335->350 WD_REPEATS PDOC00574 PS00678 376->391 WD REPEATS PDOC00574

Pfam for DKFZphutel_lι2.2

HMM_NAME WD domain, G-beta repeats

HMM *MrGHnnWVWCVaFSPDGrWFIvSGSWDgTCRLWD*

++GH ++VWC+ + G + ++SGS D+T+++WD Query 316 FVGHQGPVWCLCVYSMGDL-LFSGSSDKTIKVWD 348

22.93 519 553 1 34 dkfzphutel_lι2.2 similarity to Dictostelium myosin heavy chain kinase

Alignment to HMM consensus: Query *MrGHnnWVWCVaF.. SPDGrWFIvSGSWDgTCRLWD*

++GH ++V+++A+ +PD ++S+S D+++R+W+ dkfzphutel 519 LTGHVGTVYALAVISTPDQTK-VFSASYDRSLRVWS 553

HMM_NAME Zinc fmger, C3HC4 type (RING finger)

HMM *CPICFcTFQlDyPWPFdePmMlPCgHsFCypCIrrW..CPmC*

C++C + F++P++++CGH+FC+ C +++ CP+ Query 55 CQLC CSV FKDPVITTCGHTFCRRCALKSEKCPVD

DKFZphutel 20bl 9

group : metabolism

DKFZphute l_20bl 9 encodes a novel 486 amino acid protein with similarity to bacterial sarcosine oxidases ( EC 1 . 5 . 3 . 1 . )

The novel protein seems to be a novel enzyme with sarcosine oxidase activity .

The new protein can find application in modulation of sarcosine metabolism and as a new enzyme for biotechnologic production processes . similarity to sarcosine oxidases membrane regions : 1

Summary DKFZphutel_20bl 9 encodes a novel 486 amino acid protein, with similarity to sarcosine oxidases . similarity to sarcosine oxidases complete cDNA'' , complete eds potential start at Bp 48 , EST hits ,

Sequenced by AGOWA

Locus : unknown

Insert length : 1967 bp

Poly A stretch at pos . 1950 , no polyadenylation signal found

1 AGCGAGGCAG CAGTGCAGCT TTCAGAGGGT CCGGGCTCAG AGGGGTTATG

51 ATTCGGAGGG TTCTGCCGCA CGGCATGGGC CGGGGCCTCT TGACCCGGAG

101 GCCAGGCACG CGCAGAGGAG GCTTTTCTCT GGACTGGGAT GGAAAGGTGT

151 CTGAGATTAA GAAGAAGATC AAGTCGATCC TGCCTGGAAG GTCCTGTGAT

201 CTACTGCAAG ACACCAGCCA CCTGCCTCCC GAGCACTCGG ATGTGGTGAT

251 CGTGGGAGGT GGGGTGCTTG GCTTGTCTGT GGCCTATTGG CTGAAGAAGC

301 TGGAGAGCAG ACGAGGTGCT ATTCGAGTGC TAGTGGTGGA ACGGGACCAC

351 ACGTATTCAC AGGCCTCCAC TGGGCTCTCA GTAGGTGGGA TTTGTCAGCA

401 GTTCTCATTG CCTGAGAACA TCCAGCTCTC CCTCTTTTCA GCCAGCTTTC

451 TACGGAACAT CAATGAGTAC CTGGCCGTAG TCGATGCTCC TCCCCTGGAC

501 CTCCGGTTCA ACCCCTCGGG CTACCTCTTG CTGGCTTCAG AAAAGGATGC

551 TGCAGCCATG GAGAGCAACG TGAAAGTGCA GAGGCAGGAG GGAGCCAAAG

601 TTTCTCTGAT GTCTCCTGAT CAGCTTCGGA ACAAGTTTCC CTGGATAAAC

651 ACAGAGGGAG TGGCTTTGGC GTCTTATGGG ATGGAGGACG AAGGTTGGTT

701 TGACCCCTGG TGTCTGCTCC AGGGGCTTCG GCGAAAGGTC CAGTCCTTGG

751 GAGTCCTTTT CTGCCAGGGA GAGGTGACAC GTTTTGTCTC TTCATCTCAA

801 CGCATGTTGA CCACAGATGA CAAAGCGGTG GTCTTGAAAA GGATCCATGA

851 AGTCCATGTG AAGATGGACC GCAGCCTGGA GTACCAGCCT GTGGAATGCG

901 CCATTGTGAT CAACGCAGCC GGAGCCTGGT CTGCGCAAAT CGCAGCACTG

951 GCTGGTGTTG GAGAGGGGCC GCCTGGCACC CTGCAGGGCA CCAAGCTACC

1001 TGTGGAGCCG AGGAAAAGGT ATGTGTATGT GTGGCACTGC CCCCAGGGAC

1051 CAGGCCTAGA GACTCCGCTT GTTGCAGACA CCAGTGGAGC CTATTTTCGC

1101 CGGGAAGGAT TAGGTAGCAA CTACCTAGGT GGTCGTAGCC CCACTGAGCA

1151 GGAAGAACCG GACCCGGCGA ACCTGGAAGT GGACCATGAT TTCTTCCAGG

1201 ACAAGGTGTG GCCCCATTTG GCCCTGAGGG TCCCAGCTTT TGAGACTCTG

1251 AAGGTTCAGA GCGCCTGGGC CGGCTATTAC GACTACAACA CCTTTGACCA

1301 GAATGGCGTG GTGGGCCCCC ACCCGCTAGT TGTCAACATG TACTTTGCTA

1351 CTGGCTTCAG TGGTCACGGG CTCCAGCAGG CCCCTGGCAT TGGGCGAGCT

1401 GTAGCAGAGA TGGTACTGAA GGGCAGGTTC CAGACCATCG ACCTGAGCCC

1451 CTTCCTCTTT ACCCGCTTTT ACTTGGGAGA GAAGATCCAG GAGAACAACA

1501 TCATCTGAGC ATGTGTGCTC TGCACTGGCT CCACTGGCTT GCATCCTGGC

1551 TGTGTTCACA GCCTTGTTTG CTGCTTCCAT CTTCCCCAGT ACTGTGCCAG

1601 GCCTTCTCCC CCTCCCCAGT GTCCTCTCCT CTCAGGCAGG CCATTGCACC

1651 CATATGGCTG GGCAGGCACA GGCAGTGAGG CCGAGGCCAA TAGCGAGTGA

1701 TGAGCGGGAT CCTAGGACTG ATCTGTAGCC CATGCTGATG TCACCCACCA

1751 GGGCAATCCA TCTGGAGGCC TGAGCACCCT GGCCCAGGAC TGGCTTCATC

1801 CTGGCACTGA CCAGGAAAGA CTGCCTCTGA CCCTCTTAGC AGACAGAGCC

1851 CAGGCATGGG AGCACTCTGG GGCAGCCTGG CTCAGGTTTA TTGATTTTCG

1901 TCTGTTTACC CTATCCATTA ATCAATACAT GTAATTAACT CCTTCCCTCC

1951 AAAAAAAAAA AAAAAAA

BLAST Results o BLAST result Medline entries

No Medline entry

Peptide information for frame 3

ORF from 48 bp to 1505 bp; peptide length: 486 Category: similarity to known protein

1 MIRRVLPHGM GRGLLTRRPG TRRGGFSLDW DGKVSEIKKK IKSILPGRSC

51 DLLQDTSHLP PEHSDVVIVG GGVLGLSVAY WLKKLESRRG AIRVLVVERD

101 HTYSQASTGL SVGGICQQFS LPENIQLSLF SASFLRNINE YLAVVDAPPL

151 DLRFNPSGYL LLASEKDAAA MESNVKVQRQ EGAKVSLMΞP DQLRNKFPWI

201 NTEGVALASY GMEDEGWFDP WCLLQGLRRK VQSLGVLFCQ GEVTRFVSSS

251 QRMLTTDDKA VVLKRIHEVH VKMDRSLEYQ PVECAIVINA AGAWSAQIAA

301 LAGVGEGPPG TLQGTKLPVE PRKRYVYVWH CPQGPGLETP LVADTSGAYF

351 RREGLGSNYL GGRSPTEQEE PDPANLEVDH DFFQDKVWPH LALRVPAFET

401 LKVQSAWAGY YDYNTFDQNG VVGPHPLVVN MYFATGFSGH GLQQAPGIGR

451 AVAEMVLKGR FQTIDLSPFL FTRFYLGEKI QENNII

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_20bl9, frame 3

TREMBL :CEM04B2_4 gene: "M04B2.4"; Caenorhabditis elegans cosmid M04B2, N = 1, Score = 801, P = 9.2e-80

PIR:B71184 probable sarcosine oxidase - Pyrococcus horikoshn, N = 2, Score = 194, P = 2e-26

PIR:B69284 sarcosine oxidase, subunit beta (soxB) homolog - Archaeoglobus fulgidus, N = 3, Score = 189, P = 8.2e-22

TREMBL: AF042732_1 gene: "Bb"; product: "unknown protein"; Anopheles gambiae (Bb) gene, partial eds; and TU37B2 (TU37B2) and diphenol oxιdase-A2 (Dox-A2) genes, complete eds., N = 1, Score = 386, P = 8.7e-36

PIR:F71008 probable sarcosine oxidase - Pyrococcus horikoshn, N = 2, Score = 200, P = 4e-25

>TREMBL:CEM04B2_4 gene: "M04B2.4"; Caenorhabditis elegans cosmid M04B2 Length = 527

HSPs:

Score = 801 (120.2 bits), Expect = 9.2e-80, P = 9.2e-80 Identities = 171/433 (39%), Positives = 260/433 (60%)

Query: 61 PEHSDVVIVGGGVLGLSVAYWLKKLESRRGAIRVLVVERDHTYSQASTGLSVGGICQQFS 120

P +++VI+GGG+ G S A+WLK+ R +V+VVE + ++++ST LS GGI QQFS Sbjct: 91 PYRAEIVIIGGGLSGSSTAFWLKE-RFRDEDFKVVVVENNDVFTKSSTMLSTGGITQQFS 149

Query: 121 LPENIQLSLFSASFLRNINEYLAVVDAPPLDLRFNPSGYLLLA-SEKDAAAMESNVKVQR 179

+PE + +SLF+ FLR+ E+L ++D+ D+ F P+GYL LA ++++ M S KVQ Sbjct: 150 IPEFVDMSLFTTEFLRHAGEHLRILDSEQPDINFFPTGYLRLAKTDEEVEMMRSAWKVQI 209

Query: 180 QEGAKVSLMSPDQLRNKFPWINTEGVALASYGMEDEGWFDPWCLLQGLRRKVQSLGVLFC 239

+ GAKV L+S D+L ++P++N + V LAS G+E+EG D W LL +R K +LGV + Sbjct: 210 ERGAKVQLLSKDELTKRYPYMNVDDVLLASLGVENEGTIDTWQLLSAIREKNITLGVQYV 269

Query: 240 QGEVTRFVSSSQRM LTTDDKAVVLKRIHEVHVKMDRS-LEYQPVECAIVI 288

+GEV F R T D+ + +RI V V+ + +P+ +++

Sbjct: 270 KGEVEGFQFERHRASSEVHAFGDDATADENKLRAQRISGVLVRPQMNDASARPIRAHLIV 329

Query: 289 NAAGAWSAQIAALAGVGEGPPGTLQGTKLPVEPRKRYVYVWHCPQGPGLETPLVADTS-G 347

NAAG W+ Q+A +AG+G+G G L +P++PRKR V+V P P + P + D S G Sbjct: 330 NAAGPWAGQVAKMAGIGKGT-GLL-AVPVPIQPRKRDVFVIFAPDVPS-DLPFIIDPSTG 386

Query: 348 AYFRREGLGSNYLGGRSPTEQEEP—DPANLEVDHDFFQDKVWPHLALRVPAFETLKVQS 405

+ R+ G +L GR+P+++E+ D +NL+VD+D F K+WP L RVP F+T KV+S Sbjct: 387 VFCRQTDSGQTFLVGRTPSKEEDAKRDHSNLDVDYDDFYQKIWPVLVDRVPGFQTAKVKS 446 Query: 406 AWAGYYDYNTFDQNGVVGPHPLVVNMYFATGFSGHGLQQAPGIGRAVAEMVLKGRFQTID 465

AW+GY D NTFD V+G HPL N++ GF G+ + RA AE + G + ++ Sbjct: 447 AWSGYQDINTFDDAPVIGEHPLYTNLHMMCGFGERGVMHSMAAARAYAERIFDGAYINVN 506

Query: 466 LSPFLFTRFYLGEKIQE 482

L F R + I E Sbjct: 507 LRKFDMRRIVKMDPITE 523

Pedant information for DKFZphutel_20bl9, frame 3

Report for DKFZphutel_20bl9.3

[LENGTH] 486 [MW] 53811.85 [pi] 7.66 [HOMOL] TREMBL :CEM04B2_4 gene: "M04B2.4' Caenorhabditis elegans cosmid M04B2 le-78

[FUNCAT] c energy conversion [H. influenzae, HI0499] 8e-05

[BLOCKS] BL00677A D-amino acid oxidases proteins

[BLOCKS] BL00623A GMC oxidoreductases proteins

[BLOCKS] BL01304A

[EC] 1.5.99.2 Dimethylglycine dehydrogenase 2e-07

[PIRKW] flavoprotem 2e-07

[PIRKW] oxidoreductase 2e-07

[PROSITE] MYRISTYL 12

[PROSITE] CK2_PHOSPHO_SITE 5

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE 6

[KW] TRANSMEMBRANE 1

[KW] LOW COMPLEXITY 7.00 %

SEQ MIRRVLPHGMGRGLLTRRPGTRRGGFSLDWDGKVSEIKKKIKSILPGRSCDLLQDTSHLP SEG xxxxxxxxxxxxxxx xxxxxxx PRD ccceeecccccceeecccccccccccccccccchhhhhhhhhhccccccceeeccccccc

MEM

SEQ PEHΞDVVIVGGGVLGLSVAYWLKKLESRRGAIRVLVVERDHTYSQASTGLSVGGICQQFS

SEG xxxxxxxxxxx

PRD cccceeeeeccccchhhhhhhhhhhhhhcccceeeeeeccccccccccccccccceeeec

MEM MMMMMMMMMMMMMMMMM

SEQ LPENIQLSLFSASFLRNINEYLAVVDAPPLDLRFNPSGYLLLASEKDAAAMESNVKVQRQ

SEG

PRD ccchhhhhhhhhhhhhhhhhhhhhhhccccceeecccceeeehhhhhhhhhhhhhhhhhh

MEM

SEQ EGAKVSLMSPDQLRNKFPWINTEGVALASYGMEDEGWFDPWCLLQGLRRKVQSLGVLFCQ

SEG

PRD cccceeecccchhhhhhccccccccccccccccccccccccchhhhhhhhhhhheeeeec

MEM

SEQ GEVTRFVSSSQRMLTTDDKAVVLKRIHEVHVKMDRSLEYQPVECAIVINAAGAWSAQIAA

SEG

PRD ceeeeecccccccccccchhhhhhhhhheeeecccccccccceeeeeeecccchhhhhhh

MEM

SEQ LAGVGEGPPGTLQGTKLPVEPRKRYVYVWHCPQGPGLETPLVADTSGAYFRREGLGSNYL

SEG

PRD hhccccccccccccccccccccceeeeeeecccccccccceeeccccceeeeccccccee

MEM

SEQ GGRSPTEQEEPDPANLEVDHDFFQDKVWPHLALRVPAFETLKVQSAWAGYYDYNTFDQNG

SEG

PRD ecccccccccccccccccccchhhhhhhhhhhhhhcchhhhhhhhhhheeeeeccccccc

MEM

SEQ VVGPHPLVVNMYFATGFSGHGLQQAPGIGRAVAEMVLKGRFQTIDLSPFLFTRFYLGEKI

SEG

PRD cccccccccceeeecccccccccchhhhhhhhhhhhhhccceeeeccccccccccccccc

MEM

SEQ QENNII

SEG

PRD CCCCCC

MEM OOOOOOOOOOOOOOOOOOOOOOOO OOOOOOOOOOOOOOOOOOOOOOOO OOOOOOOOOOOOOOOOOOOOOOOO OOOOOOOOOOOOOOOOOOOOOOOO

DKFZphutel_20g21

group: signal transduction

DKFZphutel_20g21 encodes a novel 861 amino ac d protein with partial similarity to human ras inhibitor and other ras inhibitor proteins.

Ras is a signal transducting molecule involved m the receptor tyrosine kinase/RAS/Map kinase signalling cascade. Ras proteins bind GDP/GTP and show intrinsic GTPase activity. Mutations in ras, which change aa 12, 13 or 61 activate the potential of ras to transform cultured cells and are implicated in a variety of human tumours. The novel protein seems to be a new ras inhibitor protein.

The new protein can find application m modulating/blocking ras dependent signal transduction pathways .

Ras inhibitor additional 1188 Bp at 5' and 1107 at 3' end in comparison to 122483

Sequenced by AGOWA

Locus : unknown

Insert length: 4137 bp

Poly A stretch at pos. 4116, no polyadenylation signal found

1 GGGAGAACTG AAACAGGAGA TGGTGCGGAC AGATGTCAAC CTGGAAAATG

51 GCCTGGAACC CGCTGAAACC CACAGCATGG TAAGACACAA GGATGGTGGC

101 TATTCCGAGG AAGAGGACGT GAAGACCTGT GCCCGGGACT CAGGCTATGA

151 CAGCCTCTCC AACAGGCTCA GCATCTTGGA CCGGCTCCTC CACACCCACC

201 CCATATGGCT GCAGCTGAGT CTGAGTGAGG AGGAGGCAGC AGAGGTCCTG

251 CAGGCCCAGC CTCCGGGGAT CTTCCTGGTT CATAAATCTA CCAAGATGCA

301 GAAGAAAGTC CTCTCCCTCC GCCTGCCCTG TGAATTTGGG GCCCCACTCA

351 AGGAATTTGC CATAAAGGAA AGCACATACA CCTTTTCCCT GGAAGGCTCA

401 GGAATCAGTT TCGCAGATTT ATTCCGGCTC ATTGCTTTCT ACTGCATCAG

451 CAGGGATGTT CTACCATTTA CCTTGAAGTT GCCTTATGCC ATTTCAACAG

501 CCAAGTCGGA GGCTCAGCTT GAAGAACTGG CCCAGATGGG ACTAAATTTC

551 TGGAGCTCCC CAGCTGACAG CAAACCCCCG AACCTTCCAC CTCCCCATAG

601 GCCTCTTTCC TCCGACGGTG TCTGTCCTGC CTCCCTGCGT CAGCTCTGCC

651 TTATAAATGG AGTGCATTCT ATCAAAACCA GGACGCCTTC AGAGCTGGAG

701 TGCAGCCAGA CCAACGGGGC CCTGTGCTTT ATTAATCCCC TTTTCTTGAA

751 AGTGCACAGC CAGGACCTCA GTGGAGGCCT GAAACGGCCG AGCACAAGGA

801 CTCCCAACGC GAATGGCACG GAGCGGACTC GGTCCCCCCC ACCCAGGCCC

851 CCGCCACCCG CTATTAATAG TCTCCACACA AGCCCTCGGC TGGCCAGGAC

901 TGAAACCCAG ACGAGCATGC CAGAAACAGT CAACCATAAC AAACATGGGA

951 ACGTAGCTCT GCCTGGAACG AAACCAACTC CCATCCCTCC ACCCCGGCTG

1001 AAGAAGCAGG CTTCTTTTCT GGAAGCAGAG GGCGGTGCAA AGACCTTGAG

1051 CGGCGGCCGG CCGGGCGCAG GCCCGGAGCT GGAGCTGGGC ACAGCTGGCA

1101 GCCCAGGTGG GGCCCCGCCT GAGGCCGCCC CGGGGGATTG CACAAGGGCC

1151 CCGCCGCCCA GCTCTGAATC ACGGCCCCCG TGCCATGGAG GCCGGCAGCG

1201 GCTGAGCGAC ATGAGCATTT CTACTTCCTC CTCCGACTCG CTGGAGTTCG

1251 ACCGGAGCAT GCCTCTGTTT GGCTACGAGG CGGACACCAA CAGCAGCCTG

1301 GAGGACTACG AGGGGGAAAG TGACCAAGAG ACCATGGCGC CCCCCATCAA

1351 GTCCAAAAAG AAAAGGAGCA GCTCCTTCGT GCTGCCCAAG CTCGTCAAGT

1401 CCCAGCTGCA GAAGGTGAGC GGGGTGTTCA GCTCCTTCAT GACCCCGGAG

1451 AAGCGGATGG TCCGCAGGAT CGCCGAGCTT TCCCGGGACA AATGCACCTA

1501 CTTCGGGTGC TTAGTGCAGG ACTACGTGAG CTTCCTGCAG GAGAACAAGG

1551 AGTGCCACGT GTCCAGCACC GACATGCTGC AGACCATCCG GCAGTTCATG

1601 ACCCAGGTCA AGAACTATTT GTCTCAGAGC TCGGAGCTGG ACCCCCCCAT

1651 CGAGTCGCTG ATCCCTGAAG ACCAAATAGA TGTGGTGCTG GAAAAAGCCA

1701 TGCACAAGTG CATCTTGAAG CCCCTCAAGG GGCATGTGGA GGCCATGCTG

1751 AAGGACTTTC ACATGGCCGA TGGCTCATGG AAGCAACTCA AGGAGAACCT

1801 GCAGCTTGTG CGGCAGAGGA ATCCGCAGGA GCTGGGGGTC TTCGCCCCGA

1851 CCCCTGATTT TGTGGATGTG GAGAAAATCA AAGTCAAGTT CATGACCATG

1901 CAGAAGATGT ATTCGCCGGA AAAGAAGGTC ATGCTGCTGC TGCGGGTCTG

1951 CAAGCTCATT TACACGGTCA TGGAGAACAA CTCAGGGAGG ATGTATGGCG

2001 CTGATGACTT CTTGCCAGTC CTGACCTATG TCATAGCCCA GTGTGACATG

2051 CTTGAATTGG ACACTGAAAT CGAGTACATG ATGGAGCTCC TAGACCCATC

2101 GCTGTTACAT GGAGAAGGAG GCTATTACTT GACAAGCGCA TATGGAGCAC

2151 TTTCTCTGAT AAAGAATTTC CAAGAAGAAC AAGCAGCGCG ACTGCTCAGC

2201 TCAGAAACCA GAGACACCCT GAGGCAGTGG CACAAACGGA GAACCACCAA

2251 CCGGACCATC CCCTCTGTGG ACGACTTCCA GAATTACCTC CGAGTTGCAT

2301 TTCAGGAGGT CAACAGTGGT TGCACAGGAA AGACCCTCCT TGTGAGACCT

2351 TACATCACCA CTGAGGATGT GTGTCAGATC TGCGCTGAGA AGTTCAAGGT

2401 GGGGGACCCT GAGGAGTACA GCCTCTTTCT CTTCGTTGAC GAGACATGGC

2451 AGCAGCTGGC AGAGGACACT TACCCTCAAA AAATCAAGGC GGAGCTGCAC 2501 AGCCGACCAC AGCCCCACAT CTTCCACTTT GTCTACAAAC GCATCAAGAA

2551 CGATCCTTAT GGCATCATTT TCCAGAACGG GGAAGAAGAC CTCACCACCT

2601 CCTAGAAGAC AGGCGGGACT TCCCAGTGGT GCATCCAAAG GGGAGCTGGA

2651 AGCCTTGCCT TCCCGCTTCT ACATGCTTGA GCTTGAAAAG CAGTCACCTC

2701 CTCGGGGACC CCTCAGTGTA GTGACTAAGC CATCCACAGG CCAACTCGGC

2751 CAAGGGCAAC TTTAGCCACG CAAGGTAGCT GAGGTTTGTG AAACAGTAGG

2801 ATTCTCTTTT GGCAATGGAG AATTGCATCT GATGGTTCAA GTGTCCTGAG

2851 ATTGTTTGCT ACCTACCCCC AGTCAGGTTC TAGGTTGGCT TACAGGTATG

2901 TATATGTGCA GAAGAAACAC TTAAGATACA AGTTCTTTTG AATTCAACAG

2951 CAGATGCTTG CGATGCAGTG CGTCAGGTGA TTCTCACTCC TGTGGATGGC

3001 TTCATCCCTG CCTTCCTTCC TTTCTTTTTC CTTTTTTTTT TTTTTTTTTT

3051 TTTTTACAAA GAGCCTTCAT GTTTTTATAT ATTTCATAGA AATTTTTATA

3101 GCAGTTGCAG GTAAACTGTC AGGATTGGTT TTAAAATATT TTTGTAACTT

3151 TAAAATATTC TATAATTATG CATGTGATTT TAACATTTAA TATTCAAAAA

3201 TAAATCTCTT GCTGGATTTG AGAGTATTGC ATTTTTAAAG TCTCTCTTCT

3251 GTAACTGGAT GTTTTGGCAA CTTTGTGGGG AGAGACTGCT GGATTTCTTA

3301 AAGCAACGTA TTCCTGACAC TGGCCACAGA ATGCCTTTGG AAATCGGATG

3351 TACTGTTCTC TTGTTCACGT TTAGTGGTGT TTTGCTGTTT TGTTTTTTAA

3401 ACAAATGATG CTGAGAATAA GGAGAGAAAT GAATGTAGAG AGAGGTAGAG

3451 AGAGAAATAT GAACTCTAAC AAAGGACTGA GGAGTGCAGT CTGCTGGTTC

3501 AGGCTCTTCA AAAGATGTAG AAAAAGAGAT AGAAGGAACC ACCTATGCTT

3551 AAAATACTGT AAATATGCAG TGAGGTTTGG CAAAATCTAT TCCATGTGTG

3601 ATTTGCTTGT AGAAACAATT TTGAAAGCCC CTTGAGGAAA ATAAAAATCA

3651 AGAAGAACAC TTTTCTCCCT TTTCCATACA AATTAAAACT TAACAGCATC

3701 AAATTATTGG GACCAGAAAC CAAGTAATGT ATAATGTGGC TTTTGTTGAG

3751 TTAAATAAGA TGCTATATAA TGGAGAAGAA TTTGAAAATG CACAAAAAAA

3801 TCAATCTACA TTATCAGAAC CTGCAGTGAA ATTAAACTTA TGTTAAATAA

3851 AACCAGTTTG CAGGTGCACA AACTATGAGG GTCTTGTATC CACGTAACAC

3901 AGGTAGT AC AAAAACATGT TATTGTACTG TGTAAAGATG CATAGTCATC

3951 TCATTTGGTT GGCTTTGTAC CTTGTACCTT TTTTAGCCTT GGCTTTTGTT

4001 GAACTAGAAC CCTCAGCACA TACTGTGTTG TACTTTTGTA AATGATTTTT

4051 TAAATGGAAT TTTGCACATA ATACATTGTA ATACTGTATG ATAATCATGT

4101 GTGAAAATAA TTTTTGAAAT AAAAAAAAAA AAAAAAA

BLAST Results

Entry 122483 from database EMBL:

Sequence 15 from patent US 5527896.

Length = 1829

Plus Strand HSPs:

Score = 9097 (1364.9 bits), Expect = 0.0, P = 0.0

Identities = 1821/1823 (99%), Positives = 1821/1823 (99%)

Medlme entries

No Medline entry

Peptide information for frame 2

ORF from 20 bp to 2602 bp; peptide length: 861

Category: known protein

Classification: Cell signaling/communication

1 MVRTDVNLEN GLEPAETHSM VRHKDGGYSE EEDVKTCARD SGYDSLSNRL

51 SILDRLLHTH PIWLQLSLSE EEAAEVLQAQ PPGIFLVHKS TKMQKKVLSL

101 RLPCEFGAPL KEFAIKESTY TFSLEGSGIS FADLFRLIAF YCISRDVLPF

151 TLKLPYAIST AKSEAQLEEL AQMGLNFWSS PADSKPPNLP PPHRPLSΞDG

201 VCPASLRQLC LINGVHSIKT RTPSELECSQ TNGALCFINP LFLKVHSQDL

251 SGGLKRPSTR TPNANGTERT RSPPPRPPPP AINSLHTSPR LARTETQTSM

301 PETVNHNKHG NVALPGTKPT PIPPPRLKKQ ASFLEAEGGA KTLSGGRPGA

351 GPELELGTAG SPGGAPPEAA PGDCTRAPPP SSESRPPCHG GRQRLSDMSI

401 STSSSDΞLEF DRSMPLFGYE ADTNSSLEDY EGESDQETMA PPIKSKKKRS

451 SSFVLPKLVK SQLQKVSGVF SSFMTPEKRM VRRIAELSRD KCTYFGCLVQ

501 DYVSFLQENK ECHVSSTDML QTIRQFMTQV KNYLSQSSEL DPPIESLIPE

551 DQIDVVLEKA MHKCILKPLK GHVEAMLKDF HMADGSWKQL KENLQLVRQR

601 NPQELGVFAP TPDFVDVEKI KVKFMTMQKM YSPEKKVMLL LRVCKLIYTV

651 MENNSGRMYG ADDFLPVLTY VIAQCDMLEL DTEIEYMMEL LDPSLLHGEG

701 GYYLTSAYGA LSLIKNFQEE QAARLLSSET RDTLRQWHKR RTTNRTIPSV

751 DDFQNYLRVA FQEVNSGCTG KTLLVRPYIT TEDVCQICAE KFKVGDPEEY

801 SLFLFVDETW QQLAEDTYPQ KIKAELHSRP QPHIFHFVYK RIKNDPYGII 851 FQNGEEDLTT S BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_20g21, frame 2

TREMBL: RNU80076_1 product: "RIN1"; Rattus norvegicus RIN1 mRNA, complete eds., N = 3, Score = 606, P = 6.8e-97

PIR:A38637 Ras interactor RIN1 - human, N = 3, Score = 587, P = 1.9e-92

TREMBL :HSRASINL_1 product: "ras inhibitor"; Human ras inhibitor mRNA, 3' end., N = 2, Score = 592, P = 9.8e-61

SWISSPROT -RIN1_HUMAN RAS INTERACTION/INTERFERENCE PROTEIN 1 (RAS INHIBITOR JC99) (FRAGMENT)., N = 2, Score = 587, P = 4.1e-60

PIR:B38637 Ras inhibitor (clone JC265) - human (fragment), N = 1, Score = 2446, P = 4.6e-254

>PIR:B38637 Ras inhibitor (clone JC265) human (fragment) Length = 471

HSPs:

Score = 2446 (367.0 bits), Expect = 4.6e-254, P = 4.6e-254 Identities = 471/471 (100%), Positives = 471/471 (100%)

Query: 391 GRQRLSDMSISTSSSDSLEFDRSMPLFGYEADTNSSLEDYEGESDQETMAPPIKSKKKRS 450

GRQRLSDMSISTSSSDSLEFDRSMPLFGYEADTNSSLEDYEGESDQETMAPPIKSKKKRS Sbjct: 1 GRQRLSDMSISTΞSSDSLEFDRSMPLFGYEADTNSSLEDYEGESDQETMAPPIKSKKKRS 60

Query: 451 SΞFVLPKLVKSQLQKVSGVFSSFMTPEKRMVRRIAELSRDKCTYFGCLVQDYVSFLQENK 510

SSFVLPKLVKSQLQKVSGVFSSFMTPEKRMVRRIAELSRDKCTYFGCLVQDYVSFLQENK Sbjct: 61 ΞSFVLPKLVKSQLQKVSGVFSSFMTPEKRMVRRIAELSRDKCTYFGCLVQDYVSFLQENK 120

Query: 511 ECHVSSTDMLQTIRQFMTQVKNYLSQSSELDPPIESLIPEDQIDVVLEKAMHKCILKPLK 570

ECHVSSTDMLQTIRQFMTQVKNYLSQSSELDPPIESLIPEDQIDVVLEKAMHKCILKPLK Sbjct: 121 ECHVSSTDMLQTIRQFMTQVKNYLSQSSELDPPIESLIPEDQIDVVLEKAMHKCILKPLK 180

Query: 571 GHVEAMLKDFHMADGΞWKQLKENLQLVRQRNPQELGVFAPTPDFVDVEKIKVKFMTMQKM 630

GHVEAMLKDFHMADGSWKQLKENLQLVRQRNPQELGVFAPTPDFVDVEKIKVKFMTMQKM Sbjct: 181 GHVEAMLKDFHMADGSWKQLKENLQLVRQRNPQELGVFAPTPDFVDVEKIKVKFMTMQKM 240

Query: 631 YSPEKKVMLLLRVCKLIYTVMENNSGRMYGADDFLPVLTYVIAQCDMLELDTEIEYMMEL 690

YSPEKKVMLLLRVCKLIYTVMENNSGRMYGADDFLPVLTYVIAQCDMLELDTEIEYMMEL Sbjct: 241 YSPEKKVMLLLRVCKLIYTVMENNSGRMYGADDFLPVLTYVIAQCDMLELDTEIEYMMEL 300

Query: 691 LDPSLLHGEGGYYLTSAYGALSLIKNFQEEQAARLLSSETRDTLRQWHKRRTTNRTIPSV 750

LDPSLLHGEGGYYLTSAYGALSLIKNFQEEQAARLLSSETRDTLRQWHKRRTTNRTIPSV Sbjct: 301 LDPSLLHGEGGYYLTSAYGALSLIKNFQEEQAARLLSSETRDTLRQWHKRRTTNRTIPSV 360

Query: 751 DDFQNYLRVAFQEVNSGCTGKTLLVRPYITTEDVCQICAEKFKVGDPEEYSLFLFVDETW 810

DDFQNYLRVAFQEVNSGCTGKTLLVRPYITTEDVCQICAEKFKVGDPEEYSLFLFVDETW Sbjct: 361 DDFQNYLRVAFQEVNSGCTGKTLLVRPYITTEDVCQICAEKFKVGDPEEYSLFLFVDETW 420

Query: 811 QQLAEDTYPQKIKAELHSRPQPHIFHFVYKRIKNDPYGIIFQNGEEDLTTS 861

QQLAEDTYPQKIKAELHΞRPQPHIFHFVYKRIKNDPYGIIFQNGEEDLTTS Sbjct: 421 QQLAEDTYPQKIKAELHSRPQPHIFHFVYKRIKNDPYGIIFQNGEEDLTTS 471

Pedant information for DKFZphutel_20g21, frame 2

Report for DKFZphutel_20g21.2

[LENGTH] 861

[MW] 96380.26

[pi] 6.15

[HOMOL] PIR:B38637 Ras inhibitor (clone JC265) - human (fragment) 0.0

[FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YML097c] 3e-10

[ FUNCAT ] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YML097c]

3e-10

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YML097c] 3e-10

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YML097c]

3e-10

[PIRKW] alternative splicing 3e-59

[SUPFAM] Ras interactor RIN1 3e-59 [KW] All_Alpha

[KW] LOW_COMPLEXITY 11.27 %

SEQ MVRTDVNLENGLEPAETHSMVRHKDGGYSEEEDVKTCARDSGYDSLΞNRLSILDRLLHTH

SEG

PRD ccccceeeccccccccceeeeeecccccccccceeeeeeccccccchhhhhhhhhhhhhh

SEQ PIWLQLSLSEEEAAEVLQAQPPGIFLVHKSTKMQKKVLSLRLPCEFGAPLKEFAIKESTY

SEG ... xxxxxxxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhccccceeeeechhhhhhhhhhhcccccccccceeeeeeecc

SEQ TFSLEGSGISFADLFRLIAFYCISRDVLPFTLKLPYAISTAKSEAQLEELAQMGLNFWSS

SEG

PRD ceeecccccchhhhhhhhhhhhhcceeeeeecccchhhhhhhhhhhhhhhhhhccccccc

SEQ PADSKPPNLPPPHRPLSSDGVCPASLRQLCLINGVHSIKTRTPSELECSQTNGALCFINP

SEG xxxxxxxxxx

PRD cccccccccccccccccccccccchhhhhhcccccccccccccccccccccccceeeecc

SEQ LFLKVHSQDLSGGLKRPΞTRTPNANGTERTRSPPPRPPPPAINSLHTSPRLARTETQTSM

SEG xxxxxxxx

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ PETVNHNKHGNVALPGTKPTPIPPPRLKKQASFLEAEGGAKTLSGGRPGAGPELELGTAG

SEG xxxxxxxxxxx xx

PRD eeeeeccccccccccccccccccccchhhhhhhhhhhccccccccccccccceeeeeccc

SEQ SPGGAPPEAAPGDCTRAPPPΞSESRPPCHGGRQRLΞDMSISTSSSDSLEFDRSMPLFGYE

SEG xxxxxxxxxxxx xxxxxxxxxx xxxxxxxxxxxxxxxxxx

PRD ccccccccccccccccccccccccccccccccccccccccccccccceeeccccccceee

SEQ ADTNSSLEDYEGESDQETMAPPIKSKKKRSSSFVLPKLVKSQLQKVSGVFSSFMTPEKRM

SEG xxxxxxxxx

PRD cccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhcchhhh

SEQ VRRIAELSRDKCTYFGCLVQDYVSFLQENKECHVSSTDMLQTIRQFMTQVKNYLSQSSEL

SEG

PRD hhhhhhhhhhchhhhhhhhhhhhhhhhcccccccccchhhhhhhhhhhhhhhhhhhhhcc

SEQ DPPIEΞLIPEDQIDVVLEKAMHKCILKPLKGHVEAMLKDFHMADGSWKQLKENLQLVRQR

SEG

PRD ccccccccccchhhhhhhhhhhhhccccchhhhhhhhhhhhhccccchhhhhhhhhhhhh

SEQ NPQELGVFAPTPDFVDVEKIKVKFMTMQKMYSPEKKVMLLLRVCKLIYTVMENNSGRMYG

SEG

PRD ccccccccccccccchhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhcccccc

SEQ ADDFLPVLTYVIAQCDMLELDTEIEYMMELLDPSLLHGEGGYYLTSAYGALSLIKNFQEE

SEG

PRD cccccccceeecccccchhhhhhhhhhhhhhcccccccccceeeeehhhhhhhhhhhhhh

SEQ QAARLLSSETRDTLRQWHKRRTTNRTIPSVDDFQNYLRVAFQEVNSGCTGKTLLVRPYIT

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhcccccccchhhhhhhhhhhccccccceeeeecccccc

SEQ TEDVCQICAEKFKVGDPEEYSLFLFVDETWQQLAEDTYPQKIKAELHSRPQPHIFHFVYK

SEG

PRD chhhhhhhhhheeecccccceeeeehhhhhhcccccccchhhhhhhhhccccceeeehhh

SEQ RIKNDPYGIIFQNGEEDLTTS

SEG

PRD hhccccceeeeeccccccccc

(No Prosite data available for DKFZphutel_20g21.2) (No Pfam data available for DKFZphutel_20g21.2) DKFZphutel 20hl3

group: intracellular transport and trafficking

DKFZphutel_20hl3 encodes a novel 955 ammo acid protein with similarity to alpha-adaptins .

Adaptms are components of the adaptor complexes which link clathrm to receptors in coated vesicles. The alpha-adaptins, which are found exclusively in endocytic coated vesicles, separate into two bands on SDS gels, designated A and C. The novel protein is very similar to both alpha adaptin A and C. The novel protein is a new human alpha-adaptin.

The new protein can find application in modulating endocytosis and vesicle trafficking in cells . strong similarity to alpha-adaptins complete cDNA, complete eds start at Bp 78, EST hits

Sequenced by AGOWA

Locus: unknown

Insert length: 3352 bp

Poly A stretch at pos. 3297, polyadenylation signal at pos. 3279

1 GCGCCCGGTC CCCGCTTGCC AGCCCCCGCT GCTCTGTGCC CTGTCCGGCC 51 AGGCCTGGAG CCGACACCAC CGCCATCATG CCGGCCGTGT CCAAGGGCGA

101 TGGGATGCGG GGGCTCGCGG TGTTCATCTC CGACATCCGG AACTGTAAGA

151 GCAAAGAGGC GGAAATTAAG AGAATCAACA AGGAACTGGC CAACATCCGC

201 TCCAAGTTCA AAGGAGACAA AGCCTTGGAT GGCTACAGTA AGAAAAAATA

251 TGTGTGTAAA CTGCTTTTCA TCTTCCTGCT TGGCCATGAC ATTGACTTTG

301 GGCACATGGA GGCTGTGAAT CTGTTGAGTT CCAATAAATA CACAGAGAAG

351 CAAATAGGTT ACCTGTTCAT TTCTGTGCTG GTGAACTCGA ACTCGGAGCT

401 GATCCGCCTC ATCAACAACG CCATCAAGAA TGACCTGGCC AGCCGCAACC

451 CCACCTTCAT GTGCCTGGCC CTGCACTGCA TCGCCAACGT GGGCAGCCGG

501 GAGATGGGCG AGGCCTTTGC CGCTGACATC CCCCGCATCC TGGTGGCCGG

551 GGACAGCATG GACAGTGTCA AGCAGAGTGC GGCCCTGTGC CTCCTTCGAC

601 TGTACAAGGC CTCGCCTGAC CTGGTGCCCA TGGGCGAGTG GACGGCGCGT

651 GTGGTACACC TGCTCAATGA CCAGCACATG GGTGTGGTCA CGGCCGCCGT

701 CAGCCTCATC ACCTGTCTCT GCAAGAAGAA CCCAGATGAC TTCAAGACGT

751 GCGTCTCTCT GGCTGTGTCG CGCCTGAGCC GGATCGTCTC CTCTGCCTCC

801 ACCGACCTCC AGGACTACAC CTACTACTTC GTCCCAGCAC CCTGGCTCTC

851 GGTGAAGCTC CTGCGGCTGC TGCAGTGCTA CCCGCCTCCA GAGGATGCGG

901 CTGTGAAGGG GCGGCTGGTG GAATGTCTGG AGACTGTGCT CAACAAGGCC

951 CAGGAGCCCC CCAAATCCAA GAAGGTGCAG CATTCCAACG CCAAGAACGC 1001 CATCCTCTTC GAGACCATCA GCCTCATCAT CCACTATGAC AGTGAGCCCA 1051 ACCTCCTGGT TCGGGCCTGC AACCAGCTGG GCCAGTTCCT GCAGCACCGG 1101 GAGACCAACC TGCGCTACCT GGCCCTGGAG AGCATGTGCA CGCTGGCCAG 1151 CTCCGAGTTC TCCCATGAAG CCGTCAAGAC GCACATTGAC ACCGTCATCA 1201 ATGCCCTCAA GACGGAGCGG GACGTCAGCG TGCGGCAGCG GGCGGCTGAC 1251 CTCCTCTACG CCATGTGTGA CCGGAGCAAT GCCAAGCAGA TCGTGTCGGA 1301 GATGCTGCGG TACCTGGAGA CGGCAGACTA CGCCATCCGC GAGGAGATCG 1351 TCCTGAAGGT GGCCATCCTG GCCGAGAAGT ACGCCGTGGA CTACAGCTGG 1401 TACGTGGACA CCATCCTCAA CCTCATCCGC ATTGCGGGCG ACTACGTGAG 1451 TGAGGAGGTG TGGTACCGTG TGCTACAGAT CGTCACCAAC CGTGATGACG 1501 TCCAGGGCTA TGCCGCCAAG ACCGTCTTTG AGGCGCTCCA GGCCCCTGCC 1551 TGTCACGAGA ACATGGTGAA GGTTGGCGGC TACATCCTTG GGGAGTTTGG 1601 GAACCTGATT GCTGGGGACC CCCGCTCCAG CCCCCCAGTG CAGTTCTCCC 1651 TGCTCCACTC CAAGTTCCAT CTGTGCAGCG TGGCCACGCG GGCGCTGCTG 1701 CTGTCCACCT ACATCAAGTT CATCAACCTC TTCCCCGAGA CCAAGGCCAC 1751 CATCCAGGGC GTCCTGCGGG CCGGCTCCCA GCTGCGCAAT GCTGACGTGG 1801 AGCTGCAGCA GCGAGCCGTG GAGTACCTCA CCCTCAGCTC AGTGGCCAGC 1851 ACCGACGTCC TGGCCACGGT GCTGGAGGAG ATGCCGCCCT TCCCCGAGCG 1901 CGAGTCGTCC ATCCTGGCCA AGCTGAAACG CAAGAAGGGG CCAGGGGCCG 1951 GCAGCGCCCT GGACGATGGC CGGAGGGACC CCAGCAGCAA CGACATCAAC 2001 GGGGGCATGG AGCCCACCCC CAGCACTGTG TCGACGCCCT CGCCCTCCGC 2051 CGACCTCCTG GGGCTGCGGG CAGCCCCTCC CCCGGCAGCA CCCCCGGCTT 2101 CTGCAGGAGC AGGGAACCTT CTGGTGGACG TCTTCGATGG CCCGGCCGCC 2151 CAGCCCAGCC TGGGGCCCAC CCCCGAGGAG GCCTTCCTCA GCCCAGGTCC 2201 TGAGGACATC GGCCCTCCCA TTCCGGAAGC CGATGAGTTG CTGAATAAGT 2251 TTGTGTGTAA GAACAACGGG GTCCTGTTCG AGAACCAGCT GCTGCAGATC 2301 GGAGTCAAGT CAGAGTTCCG ACAGAACCTG GGCCGCATGT ATCTCTTCTA 2351 TGGCAACAAG ACCTCGGTGC AGTTCCAGAA TTTCTCACCC ACTGTGGTTC 2401 ACCCGGGAGA CCTCCAGACT CAGCTGGCTG TGCAGACCAA GCGCGTGGCG 2451 GCGCAGGTGG ACGGCGGCGC GCAGGTGCAG CAGGTGCTCA ATATCGAGTG 2501 CCTGCGGGAC TTCCTGACGC CCCCGCTGCT GTCCGTGCGC TTCCGGTACG 2551 GTGGCGCCCC CCAGGCCCTC ACCCTGAAGC TCCCAGTGAC CATCAACAAG 2601 TTCTTCCAGC CCACCGAGAT GGCGGCCCAG GATTTCTTCC AGCGCTGGAA

2651 GCAGCTGAGC CTCCCTCAAC AGGAGGCGCA GAAAATCTTC AAAGCCAACC

2701 ACCCCATGGA CGCAGAAGTT ACTAAGGCCA AGCTTCTGGG GTTTGGCTCT

2751 GCTCTCCTGG ACAATGTGGA CCCCAACCCT GAGAACTTCG TGGGGGCGGG

2801 GATCATCCAG ACTAAAGCCC TGCAGGTGGG CTGTCTGCTT CGGCTGGAGC

2851 CCAATGCCCA GGCCCAGATG TACCGGCTGA CCCTGCGCAC CAGCAAGGAG

2901 CCCGTCTCCC GTCACCTGTG TGAGCTGCTG GCACAGCAGT TCTGAGCCCT

2951 GGACTCTGCC CCGGGGGATG TGGCCGGCAC TGGGCAGCCC CTTGGACTGA

3001 GGCAGTTTTG GTGGATGGGG GACCTCCACT GGTGACAGAG AAGACACCAG

3051 GGTTTGGGGG ATGCCTGGGA CTTTCCTCCG GCCTTTTGTA TTTTTATTTT

3101 TGTTCATCTG CTGCTGTTTA CATTCTGGGG GGTTAGGGGG AGTCCCCCTC

3151 CCTCCCTTTC CCCCCCAAGC ACAGAGGGGA GAGGGGCCAG GGAAGTGGAT

3201 GTCTCCTCCC CTCCCACCCC ACCCTGTTGT AGCCCCTCCT ACCCCCTCCC

3251 CATCCAGGGG CTGTGTATTA TTGTGAGCGA ATAAACAGAG AGACGCTAAA

3301 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

3351 AA

BLAST Results

No BLAST result

Medl e entries

89155572:

Cloning of cDNAs encoding two related 100-kD coated vesicle proteins

(alpha-adaptins) .

97431776:

Alpha-adaptm, a marker for endocytosis, is expressed m complex patterns during Drosophila development .

Peptide information for frame 3

ORF from 78 bp to 2942 bp; peptide length: 955 Category: strong similarity to known protein

1 MPAVSKGDGM RGLAVFISDI RNCKSKEAEI KRINKELANI RSKFKGDKAL 51 DGYSKKKYVC KLLFIFLLGH DIDFGHMEAV NLLSSNKYTE KQIGYLFISV 101 LVNSNSELIR LINNAIKNDL ASRNPTFMCL ALHCIANVGS REMGEAFAAD 151 IPRILVAGDS MDSVKQSAAL CLLRLYKASP DLVPMGEWTA RVVHLLNDQH 201 MGVVTAAVSL ITCLCKKNPD DFKTCVSLAV SRLSRIVSSA STDLQDYTYY 251 FVPAPWLSVK LLRLLQCYPP PEDAAVKGRL VECLETVLNK AQEPPKSKKV 301 QHSNAKNAIL FETISLIIHY DSEPNLLVRA CNQLGQFLQH RETNLRYLAL 351 ESMCTLAΞSE FSHEAVKTHI DTVINALKTE RDVSVRQRAA DLLYAMCDRS 401 NAKQIVSEML RYLETADYAI REEIVLKVAI LAEKYAVDYS WYVDTILNLI 451 RIAGDYVSEE VWYRVLQIVT NRDDVQGYAA KTVFEALQAP ACHENMVKVG 501 GYILGEFGNL IAGDPRSSPP VQFSLLHSKF HLCSVATRAL LLSTYIKFIN 551 LFPETKATIQ GVLRAGSQLR NADVELQQRA VEYLTLSSVA STDVLATVLE 601 EMPPFPERES SILAKLKRKK GPGAGSALDD GRRDPSSNDI NGGMEPTPST 651 VSTPSPSADL LGLRAAPPPA APPASAGAGN LLVDVFDGPA AQPSLGPTPE 701 EAFLSPGPED IGPPIPEADE LLNKFVCKNN GVLFENQLLQ IGVKSEFRQN 751 LGRMYLFYGN KTSVQFQNFS PTVVHPGDLQ TQLAVQTKRV AAQVDGGAQV 801 QQVLNIECLR DFLTPPLLSV RFRYGGAPQA LTLKLPVTIN KFFQPTEMAA 851 QDFFQRWKQL SLPQQEAQKI FKANHPMDAE VTKAKLLGFG SALLDNVDPN 901 PENFVGAGII QTKALQVGCL LRLEPNAQAQ MYRLTLRTSK EPVSRHLCEL 951 LAQQF

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_20hl3, frame 3

PIR:B30111 alpha-adaptm C - mouse, N = 1, Score = 3990, P = 0

PIR:S11276 alpha-adaptm c - rat, N = 1, Score = 3987, P = 0

SWISSPROT :ADAC_RAT ALPHA-ADAPTIN C (CLATHRIN ASSEMBLY PROTEIN COMPLEX 2 ALPHA-C LARGE CHAIN) (100 KD COATED VESICLE PROTEIN C) (PLASMA MEMBRANE ADAPTOR HA2/AP2 ADAPTIN ALPHA C SUBUNIT)., N = 1, Score = 3982, P = 0 SWISSPROT :ADAC_MOUSE ALPHA-ADAPTIN C (CLATHRIN ASSEMBLY PROTEIN COMPLEX 2 ALPHA-C LARGE CHAIN) (100 KD COATED VESICLE PROTEIN C) (PLASMA MEMBRANE ADAPTOR HA2/AP2 ADAPTIN ALPHA C SUBUNIT)., N = 1, Score = 3976, P = 0

TREMBL :AB020706_1 gene: "KIAA0899"; product: "KIAA0899 protein"; Homo sapiens mRNA for KIAA0899 protein, partial eds., N = 1, Score = 3932, P = 0

>PIR:B30111 alpha-adaptm C - mouse Length = 938

HSPs:

Score = 3990 (598.6 bits), Expect = O.Oe+00, P = O.Oe+00 Identities = 787/955 (82%), Positives = 858/955 (89%)

Query: 1 MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVC 60

MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVC Sbjct: 1 MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVC 60

Query: 61 KLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDL 120

KLLFIFLLGHDIDFGHMEAVNLLSSN+YTEKQIGYLFIΞVLVNSNSELIRLINNAIKNDL Sbjct: 61 KLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDL 120

Query: 121 ASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASP 180

ASRNPTFM LALHCIANVGSREM EAFA +IP+ILVAGD+MDSVKQSAALCLLRLY+ SP Sbjct: 121 ASRNPTFMGLALHCIANVGSREMAEAFAGEIPKILVAGDTMDΞVKQSAALCLLRLYRTSP 180

Query: 181 DLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSA 240

DLVPMG+WT+RVVHLLNDQH+GVVTAA SLIT L +KNP++FKT VSLAVSRLSRIV+SA Sbjct: 181 DLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSA 240

Query: 241 STDLQDYTYYFVPAPWLΞVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKV 300

STDLQDYTYYFVPAPWLSVKLLRLLQCYPPP D AV+GRL ECLET+LNKAQEPPKSKKV Sbjct: 241 STDLQDYTYYFVPAPWLSVKLLRLLQCYPPP-DPAVRGRLTECLETILNKAQEPPKSKKV 299

Query: 301 QHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSE 360

QHSNAKNA+LFE ISLIIH+DSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSE Sbjct: 300 QHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSE 359

Query: 361 FSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAI 420

FSHEAVKTHI+TVINALKTERDVSVRQRA DLLYAMCDRSNA+QIV+EML YLETADY+I Sbjct: 360 FSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAQQIVAEMLSYLETADYSI 419

Query: 421 REEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAA 480

REEIVLKVAILAEKYAVDY+WYVDTILNLIRIAGDYVSEEVWYRV+QIV NRDDVQGYAA Sbjct: 420 REEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAA 479

Query: 481 KTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRAL 540

KTVFEALQAPACHEN+VKVGGYILGEFGNLIAGDPRSSP +QF+LLHSKFHLCSV TRAL Sbjct: 480 KTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFNLLHSKFHLCSVPTRAL 539

Query: 541 LLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLE 600

LLSTYIKF+NLFPE KATIQ VLR+ SQL+NADVELQQRAVEYL LS+VASTD+LATVLE Sbjct: 540 LLSTYIKFVNLFPEVKATIQDVLRSDSQLKNADVELQQRAVEYLRLSTVASTDILATVLE 599

Query: 601 EMPPFPERESSILAKLKRKKGPGAGSALDDGRRDPSSNDINGGMEPTP STVSTPSPS 657

EMPPFPERESSILAKLK+KKGP + L++ +R+ S D+NGG EP P S STPSPS Sbjct: 600 EMPPFPERESSILAKLKKKKGPSTVTDLEETKRERSI-DVNGGPEPVPASTSAASTPSPS 658

Query: 658 ADLLGLRAAPP-PAAPPASAGAGNLLVDVFDGPAAQPSLGPTPEEAFLSPGPEDIGPPIP 716

ADLLGL A PP P PP S+G G LLVDVF A+ ++ P L+PG ED Sbjct: 659 ADLLGLGAVPPAPTGPPPSSGGG-LLVDVFSDSAS--AVAP LAPGSEDN 704

Query: 717 EADELLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHP 776

+FVCKNNGVLFENQLLQIG+KSEFRQNLGRM++FYGNKTS QF NF+PT++ Sbjct: 705 FARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICA 759

Query: 777 GDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLP 836

DLQT L +QTK V VDGGAQVQQV+NIEC+ DF P+L+++FRYGG Q +++KLP Sbjct: 760 DDLQTNLNLQTKPVDPTVDGGAQVQQVVNIECISDFTEAPVLNIQFRYGGTFQNVSVKLP 819

Query: 837 VTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDN 896

+T+NKFFQPTEMA+QDFFQRWKQLS PQQE Q IFKA HPMD E+TKAK++GFGSALL+ Sbjct: 820 ITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEITKAKIIGFGSALLEE 879

Query: 897 VDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF 955 VDPNP NFVGAGII TK Q+GCLLRLEPN QAQMYRLTLRTSK+ VS+ LCELL++QF Sbjct: 880 VDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKDTVSQRLCELLSEQF 938

Pedant information for DKFZphutel_20hl3, frame 3

Report for DKFZphutel_20hl3.3

[LENGTH] 955

[MW] 105361.97

[pi] 7.75

[HOMOL] PIR:A30111 alpha-adaptin A - mouse 0.0

[FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae,

YBL037w] 5e-67

[FUNCAT] 08.19 cellular import [S. cerevisiae, YBL037w] 5e-67

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YBL037w] 5e-67

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDR238c]

4e-04

[PIRKW] heterodimer 0.0

[PIRKW] transmembrane protein le-65

[PIRKW] membrane trafficking 0.0

[PIRKW] receptor 0.0

[SUPFAM] beta-adaptin 5e-16

[PROSITE] MYRISTYL 7

[PROSITE] IG_MHC 1

[PROSITE] AMIDATION 1

[PROSITE] CK2_PHOSPHO_SITE 11

[PROSITE] TYR_PHOSPHO_SITE 3

[PROSITE] PKC_PHOSPHO_SITE 15

[PROSITE] ASN_GLYCOSYLATION 1

[KW] All_Alpha

[KW] LOW_COMPLEXITY 6.81 %

SEQ MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVC SEG

PRD ccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccchhhhhhh

SEQ KLLFIFLLGHDIDFGHMEAVNLLSΞNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDL SEG

PRD hhhhhhhcccccccchhhhhhhhhcccccchhhhhhhhhhhhhcchhhhhhhhhhhhhcc

SEQ ASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASP SEG

PRD cccccchhhhhhhhhhccchhhhhhhhhhhhhheeeccccchhhhhhhhhhhhhhhhhcc

SEQ DLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSA SEG

PRD cccccccchhhhhhhhhcccceeeehhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhcc

SEQ STDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKV SEG

PRD ccccccceeeecccchhhhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhhhccccccc

SEQ QHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSE SEG

PRD cccccchhhhhhhhhhhhhcccccceeeeehhhhhhhhhhccccceeeehhhhhhhhhcc

SEQ FSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAI SEG

PRD cchhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhcccch

SEQ REEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAA SEG

PRD hhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhccccchhhhhhhheeeccccchhhhhh

SEQ KTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRAL SEG

PRD hhhhhhhhhhcccccceeeeeeeecccccccccccccccchhhhhhhhhhhcccchhhhh

SEQ LLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLE SEG

PRD hhhhhhhhhhccccchhhhhhhhhhhcccchhhhhhhhhhhhhhhhhccchhhhhhhhhh

SEQ EMPPFPERESSILAKLKRKKGPGAGSALDDGRRDPSSNDINGGMEPTPSTVSTPSPSADL

SEG xxxxxxxxxxxxxxx

PRD hccccccchhhhhhhhhhccccccccccccccccccccccccccccccccccccccccce

SEQ LGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPEEAFLΞPGPEDIGPPIPEADE SEG xxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxx. PRD eecccccccccccccccccceeeeeeccccccccccccccceeecccccccccccccccc

SEQ LLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQ SEG PRD cceeeeeccccccchhhhhhhhcchhhhhccccceeeccccccccccccceeeeccchhh

SEQ TQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLPVTIN SEG xxxxxxxxxxxxxx PRD hhhhhhhhcccccccccchhhhhhhhhhccccccccceeeeeeccccccccccccccccc

SEQ KFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPN SEG PRD cccccchhhhhhhhhhhhhhhchhhhhhhhhhhcccchhhhhhhhhhccccceeeecccc

SEQ PENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF SEG PRD ccceeeceeeeeccccceeeeecccchhhhhhhhhhhccccchhhhhhhhhhccc

Prosite for DKFZphutel_20hl3.3

PS00001 760->764 ASN_GLYCOSYLATION PDOC00001 PS00005 54->57 PKC_PHOSPHO_SITE PDOC00005 PS00005 85->88 PKC_PHOSPHO_SITE PDOC00005 PS00005 89->92 PKC_PHOSPHO_SITE PDOC00005 PS00005 163->166 PKC_PHOSPHO_SITE PDOC00005 PS00005 189->192 PKC_PHOSPHO_SITE PDOC00005 PS00005 258->261 PKC_PHOSPHO_SITE PDOC00005 PS00005 297->300 PKC_PHOSPHO_SITE PDOC00005 PS00005 379->382 PKC_PHOSPHO_SITE PDOC00005 PS00005 384->387 PKC_PHOSPHO_SITE PDOC00005 PS00005 470->473 PKC_PHOSPHO_SITE PDOC00005 PS00005 787->790 PKC_PHOSPHO_SITE PDOC00005 PS00005 819->822 PKC_PHOSPHO_SITE PDOC00005 PS00005 832->835 PKC_PHOSPHO_SITE PDOC00005 PS00005 935->938 PKC_PHOSPHO_SITE PDOC00005 PS00005 938->941 PKC_PHOSPHO_SITE PDOC00005 PS00006 5->9 CK2_PHOSPHO_SITE PDOC00006 PS00006 104->108 CK2_PHOSPHO_SITE PDOC00006 PS00006 368->372 CK2_PHOSPHO_SITE PDOC00006 PS00006 379->383 CK2_PHOSPHO_SITE PDOC00006 PS00006 470->474 CK2_PHOSPHO_SITE PDOC00006 PS00006 482->486 CK2_PHOSPHO_SITE PDOC00006 PS00006 597->601 CK2_PHOSPHO_SITE PDOC00006 PS00006 626->630 CK2_PHOSPHO_SITE PDOC00006 PS00006 636->640 CK2_PHOSPHO_SITE PDOC00006 PS00006 698->702 CK2_PHOSPHO_SITE PDOC00006 PS00006 938->942 CK2_PHOSPHO_SITE PDOC00006 PS00007 388->395 TYR_PHOSPHO_SITE PDOC00007 PS00007 411->419 TYR_PHOSPHO_SITE PDOC00007 PS00007 434->443 TYR_PHOSPHO_SITE PDOC00007 PS00008 202->208 MYRISTYL PDOC00008 PS00008 508->514 MYRISTYL PDOC00008 PS00008 561->567 MYRISTYL PDOC00008 PS00008 623->629 MYRISTYL PDOC00008 PS00008 759->765 MYRISTYL PDOC00008 PS00008 826->832 MYRISTYL PDOC00008 PS00008 908->914 MYRISTYL PDOC00008 PS00009 630->634 AMIDATION PDOC00009 PS00290 127->134 IG MHC PDOC00262

(No Pfam data available for DKFZphutel_20hl3.3) DKFZphutel_20mll

group: cell cycle

DKFZphutel_20mll encodes a novel 225 amino acid protein with similarity to yeast sds22 and protein phosphatase-1 regulatory subunits. sds22 is a regulatory polypeptide of protein phosphatase-1 that is required for the completion of mitosis in both fission and budding yeast. The novel protein seems to be a new regulator protein for protein phosphatase-1.

The new protein can find application in modulating/blocking the activity of protein phosphatase-1 and in modulating the cell cycle. similarity to suppressor protein sds22 complete cDNA, complete eds, EST hits localisation? only a part of the STS matches

Sequenced by AGOWA

Locus: /map="17"?

Insert length: 5822 bp

Poly A stretch at pos. 5803, polyadenylation signal at pos. 5786

1 GGGCGCTTGG TTCCCCAGCA ACCGGGAGAC GCGTCTGCTG CGTGGAACCG 51 CCGAGTTCCC AGCGCTTGAG AAGGAAAATT CTGGATCTGT TATCTGTGAG

101 GAGGCCACTC CGTTGACAGT TGTGTAAAAC TCTGCTGCTT TCCCCAGCTC

151 CAACCTCTCT GGTCTTCAAC AACACTATCA TCAGGGAAAA CGTGGGGGAA

201 GATGAACCAG CCGTGCAACT CGATGGAGCC GAGGGTGATG GACGATGACA

251 TGCTCAAGCT GGCCGTCGGG GACCAGGGCC CCCAGGAGGA GGCCGGGCAG

301 CTGGCCAAGC AGGAGGGCAT CCTCTTCAAG GATGTCCTGT CCCTGCAGCT

351 GGACTTTCGG AACATCCTCC GCATAGACAA CCTCTGGCAG TTTGAGAACT

401 TGAGGAAGCT GCAGCTGGAC AATAACATCA TTGAGAAGAT CGAGGGCCTG

451 GAGAACCTCG CACACCTGGT CTGGCTGGAT CTGTCTTTCA ACAACATTGA

501 GACCATCGAG GGGCTGGACA CACTGGTGAA CCTGGAGGAC CTGAGCTTGT

551 TCAACAACCG GATCTCCAAG ATCGACTCCC TGGACGCCCT CGTCAAGCTG

601 CAGGTGTTGT CGCTGGGCAA CAACCGGATT GACAACATGA TGAACATCAT

651 CTACCTCCGG CGGTTCAAGT GCCTGCGGAC GCTCAGCCTC TCTAGGAACC

701 CTATCTCTGA GGCAGAGGAT TACAAGATGT TCATCTGTGC CTACCTTCCT

751 GACCTCATGT ACCTGGACTA CCGGCGCATT GATGACCACA CAGCAAGTGT

801 CTCCCTCTCA GTCTCCCAGC CCTGTGAGAC AGATTCCTCA AGCCCCCAGG

851 TTTCTTGGAA AAGGGGCATT GAAGAGTAGC TTCCCCTGCC CACAACTAGG

901 AGAGAAAGGG CAGCTCCCTC TTCCTAATCC CTTTACCTGA CTCTGTCAGA

951 GTGATTCCAG CAGCACCCTT GTAAGTACTG TTTTGTGTGC GTTCCCAGGG 1001 GCCAGGCCTC TTCCACACAC TGTCCCAGGG CCACCTCACA GCCATCCTGC 1051 ACTGTCTAGT TTTCCAGATG AAGAAGCTGA GGAGGGCTGG GAGCAGTGGC 1101 TCACGCCTGT AATCCCAGCA CTTTGAGAGG CTGAGGCGGG AGGATCGCTT 1151 GAGCCAAGGA GTTCAAGACC AGCCTGGGCA ACATAGGGAG ACCCCATCTC 1201 TACAGAAACT ACCAAAATTA GCCAGGTGTG GTGGCACACA CCAGTAATCC 1251 TGGCTACTCA CAAGGCCGAG GTAGAAGAAT CGCTTGAGAC TAGGAGTTTG 1301 AGGCTGCAGT GAACTAAGAA GATGCCATTG CACTCCAGCC TGGGCAACAG 1351 AGTGAAAAAA TTAAAAAATT AGAAAAGAAA AGAAGTTGAG GAGGCCCAAG 1401 GAGGGCAAGC AGCCAGGATC ACTGGCTCAA GGCCAAGCCA GGATTCACCC 1451 TAAGTTGGTG TCATCCCAGG AGCAATATTA ACAGCTGAGC TCCAGAGGGA 1501 ACCAGGCCAT CAGAGGCTCA GGCCTGGCTC TCAGGGGCAG AGTCAGGGCT 1551 GGAGGTAGAG ACCTGAGTGT CATCTGAGGA TTGCCAATTG GCAGTAGTTG 1601 AAGCCATGGT ACAGGTGGGA TCACCTGGGG CACATGGAGT GAGCTGGGGG 1651 ACGGGGACTA AGTTCTAGAG GTGCCAGCAT TCCTGGCCAG GTACAGGGGG 1701 ATGAGCCAGT GCGGTGGAGA GAGCCAAGGG CCAGACCCTC GTGACCAGCC 1751 CTATGGCCTC ACTCTACCTC TGTCCTGTTG TCCTCCTTCC CTAAAAGAGG 1801 GCCAGAAGGC CTGCTGAGGG CTGTTGGGAG TGAGAGAGCA AGTCCTCTGT 1851 GGAGAACACC CAGTCTGGGG CGAGGGGAGC GCTCCATTGC TGTGGCTCCT 1901 GCCCTGGAGA TGGCCCCGGG AACCCCAGCC TGCCACGCTG CCTTCCGCTC 1951 CTCCTGGTCT TTCCCTGATT TCCCTGCGCT CACAAAAACC TGGTGAGGGT 2001 CATCAGGAGA TGGGCATTCT CATCCACGAG ACCTCATGGC TTTCACAGCC 2051 TTCATGCAGG CCCCTGTGCA ACACCCCTGC CCATGCGCGG GAGGCTGCAG 2101 CATGGCAGAG GCGGCATGGC AGAGGCGGTG TGGCTCGGAG GAACCTCTGG 2151 TAACAATGCC ACTCCCGTTC CCTGGTCAGA AAAAGCTTGC GGAGGCTAAG 2201 CACCAGTACA GCATCGACGA GCTGAAGCAC CAGGAGAACC TGATGCAGGC 2251 CCAGCTGGAG GACGAGCAGG CGCAGCGGGA GGAGCTAGAG AAGCACAAGA 2301 CTGCGTTTGT GGAACACCTG AATGGCTCCT TCCTGTTTGA CAGCATGTAC 2351 GCTGAGGACT CAGAGGGCAA CAATCTGTCC TACCTGCCTG GTGTCGGTGA 2401 GCTCCTTGAG ACCTACAAGG ACAAGTTTGT CATCATCTGC GTGAATATTT 2451 TTGAGTATGG CCTGAAACAG CAGGAGAAGC GGAAAACAGA GCTTGACACC 2501 TTCAGTGAAT GTGTCCGTGA GGCCATCCAG GAAAACCAGG AGCAGGGCAA 2551 ACGCAAGATT GCCAAATTCG AGGAGAAGCA CTTGTCGAGT TTAAGTGCCA

2601 TTCGAGAGGA GTTGGAACTG CCCAACATTG AGAAGATGAT CCTAGAATGC

2651 AGTGCTGACA TCAGTGAGTT GTTCGATGCG CTCATGACGC TGGAGATGCA

2701 GCTGGTGGAG CAGCTGGAGG TAAGGCTGGG CCCTGGGCAC AAGTGCCAGA

2751 ATCTGGCGAT GCAGCTGCAC ATCCATAGGT GAACTGTAGC CTTCATGGGC

2801 ACGCCTCTGC TGGAAACGTC CAGCACGACT CAGCGTGGCA GGCTGTAGCT

2851 TTCTTGCTCA TCAGTCCTGT TTGCTTTTAT TACATTTTAA TCATTTACAT

2901 TGGAAGTGAT TCTTGTGGAA AATGAGAGGT GAGCTCATTC TTCTGAAATG

2951 GTCCCCCTAT CCTGGAAGTC AGTGGGGAGA GGTTTTTGAT TAGACCCCTG

3001 GAGCTATCCG GGTACTCTAA AGGCAAAGCG CACCCCCACT TGGGGACCAA

3051 ACAAAGACCC CTCCGCATTG CAGCCTGCAG TTGCCGCTTC TCAGGTGACG

3101 TGAGGAGGCT GCAACTCAGC ACTAAGTAGT GAAAATGAAA AGCGCCGCTG

3151 TCTGAAATTC ATTAGCAGCC AGAGTATGTG TTACAAGGCA GCGGAGGCTG

3201 GGAGTCTGAA GTGGTGTGAT GAATTGAACC TCATCGGATG CTGCTGTGGC

3251 TGGGCCAAGT GATAGCACCT AATCAATTCC TCACACGTCA AGTGACACCT

3301 CAGACATGGG ATAGATTTCC CCATCACATC ACAGGGCAGG TGCTCCCTCC

3351 CTGCTGGAGA GCACAGGCAC TGCAGAAGCA GCGCACAGTG CCAGGGGCGA

3401 GTGAGGCAGC AGCTCCCAGC CTTTTCAGGC ACGGAGATTG CCTTTCAACA

3451 TCCAAACATT TCCCAGAACC CATGTGCCAT CCTACTTGTA TTACTGGTGG

3501 CCAGAAAGCC ACAAGCGCAA TCATGCTTTT CAATGACCCT ATTTTTATTC

3551 ACGAGAACAG CACATACATG TGTTTGAAAA TTATGTGAGG TGCTCACTCT

3601 GCAGACAGTA CTCACATTCC TATAGATTCC ACCCCTGCCC ACCTTGCAGC

3651 CCCTGGAGTC TATAGCAGAT GGGAGTGGGG CACTCCGAGA GTGGCAGGCC

3701 TGGAGATCAC ATCTTCCATT GTTCCTTCAA TCAACACTAA CTCCCATTTG

3751 GGCCTTAGGT GCCTTGCTAA GCACCACAAA ACAGCAACTA ACTGAAAGAG

3801 ATCTGGAGTG CCAGCCCGCT CCTACTGAGG GCCTCCTCTC TGTCAGGCAC

3851 CTTGCAAAGC ATTTTGTGTG AAGTGACTCA TTTAACCTCA CCACAACGCC

3901 ACAACGCAGG GATTATGCAG GTAACCTATT TCCCAGATGA GGAAGATAAG

3951 GCCCAAGGAG GTGAAATGCC TTTCCCAGAG TTACACAGAG TGCTGGAGCT

4001 GGGAATACTG ACCCAGGCAG TCTAGCTCTT AACAGCTCAC TCCACTGTTT

4051 CCCTGGAGGT GATGCACAGA TGTCACTGGG AAACCCAAAG GAGAGGGGGT

4101 TGGCTGTGTG TGTGTGTGTT GGGCAGGCAG GTAAGGGGAG TAAGACCAGG

4151 ACAAGTGTTC CTGGCAAAGT TCCGGTGACA GCATTAAACA TTCAGATGGT

4201 GAGGGAGTTA ATATGGTTGG AGAACAACAA CTTTAGAGAG AGCAGAGGGG

4251 TCAGTTCACA ACCATCTGCT CAGGAGGGTC AAGATGGGTG GTCTTTATGC

4301 TGAAGGTCTG TGATTAGAGG AGCTGGTTGC TAAATTTTGA GGAGTACCTT

4351 TTGCTCTGTG CTGGACATCT AAATATGCAT GTTAACTGTG TTCTTTAACA

4401 TTTCCAGGAG ACTATAAACA TGTTTGAAAG GAACATTGTT GACATGGTAG

4451 GACTGTTTAT CGAAAATGTC CAAAGCCTAT ATCCTTTCTG TGATGACCTT

4501 CCCCATGGGG AGGTGCTACA GAGCCCCTGG GCTTGTCCCG GCCTCTGGAC

4551 AAAAGAATGT TCCACAGGGT CTGAGGAGGT TTCCCGACCC TCAGAACAAT

4601 GATGGCCTGG TTAGAGCTGT GGTTTGGATG CCCAGAGGGA CAACATCCAA

4651 ACTGTTTGCA GTAGGCTCCC AGCATGATTG TTCTCATATG AGTGATGTTC

4701 ACTAGGAAAT GACGCCCCCT GTGTTGCAGG CAAGCACACT CTGGGGTTGA

4751 GGCAACCCCC ACGTGGAAGA CACTATAAGG AGTACATCAG GTGAAATGTT

4801 AGGGTGAGGA GCCAACATCG GAGCATGGCC AACCCTTCTT CCACCCGAAC

4851 TCAGGGCACT CCACATGGGG CAAACTGCTG TGCTCCAGCT AGCAGCAGCC

4901 CTGTGGTCCT GCCCTCCTGG GGCTCACAGT CCCTCAGGGA GACAAGTTGT

4951 AGAGGCAACA AGTGGTGCCA AATGCACAGG GTGAGAAGCA GTTAACCCAG

5001 AGGCCAGGAG CCTCCATGCA GGAGGGAGAG AAGAGTGTGA TGGCAGGGGC

5051 CGAGGGTCCG TCCGAGGTGT GGGGCAGGGG CAGGGAGTCG AGGAAGGCCC

5101 AGGGTTCGGA GCTTGTGAGT GGACGGTGCT GCCAGCCAGA ATTTCCGAGC

5151 TCGCCTTGGG CCCTTAAAGT CTGTCTCCCG CCGTCTGAGA GCATCAGGGA

5201 CGCGCCGGGC CTGCTCCTCC CGGGCCTTTG CTTAACTCGG GGCTGCACGA

5251 TGGCTCAGTG CCGGGACCTG GAGAATCACC ACCACGAGAA GCTCCTGGAG

5301 ATCTCTATCA GCACCCTGGA GAAGATTGTC GAGGGCGACC TGGACGAGGA

5351 CCTGCCTAAC GACCTGCGCG CGCTTTTTGT CGATAAAGAT ACGATTGTTA

5401 ATGCTGTCGG GGCATCGCAC GACATCCACC TCCTGAAGAT TGACAATCGA

5451 GAAGATGAGC TGGTGACCAG AATCAACTCT TGGTGTACAC GTTTAATAGA

5501 CAGGATTCAC AAGGATGAGA TCATGAGGAA CCGCAAGCGC GTGAAGGAGA

5551 TCAATCAGTA CATCGACCAC ATGCAGAGCG AACTGGACAA CCTGGAATGT

5601 GGCGACATCC TAGACTAGAT GAATGTCAGC CACAGGAGCT TCTTCAAAAC

5651 ATAGCACCAG CCCCAGCCAG GAGAAGGAAG TGCACACGCC TCACCCGCAC

5701 CTCTAGAGAG TTGCTGGGCA TCTCTCAACC GCGATCCCCA ACACCATTCT

5751 TCCCCCACCC CTGGAAAAAC TTCCAAAAGT AGAGAAAATA AAGGACTCAT

5801 TTCACAAAAA AAAAAAAAAA AA

BLAST Results

Entry HS1292248 from database EMBL: human STS SHGC-53917. Score = 874, P = 3.3e-33, identities = 180/185

Medline entries

No Medline entry Peptide information for frame 1

ORF from 202 bp to 876 bp; peptide length: 225 Category: similarity to known protein

1 MNQPCNSMEP RVMDDDMLKL AVGDQGPQEE AGQLAKQEGI LFKDVLSLQL

51 DFRNILRIDN LWQFENLRKL QLDNNIIEKI EGLENLAHLV WLDLSFNNIE

101 TIEGLDTLVN LEDLSLFNNR ISKIDSLDAL VKLQVLSLGN NRIDNMMNII

151 YLRRFKCLRT LSLSRNPISE AEDYKMFICA YLPDLMYLDY RRIDDHTASV

201 SLSVSQPCET DSSSPQVSWK RGIEE

BLASTP hits

Entry S68209 from database PIR: sds22 protein homolog - human >TREMBL:HSSDS22MR_1 gene: "sds22"; product: "yeast sds22 homolog"; H. sapiens sds22-lιke mRNA

Score = 234, P = 1.2e-19, identities = 61/143, positives = 93/143

Entry A38439 from database PIR: suppressor protein sds22(+) - fission yeast (Schizosaccharomyces pombe) >TREMBL:SPSDS22_1 gene: "sds22+"; S. pombe sds22+ gene, complete eds. Score = 208, P = 5.6e-17, identities = 52/127, positives = 71/127

Entry S43988 from database PIR: protein suppressor sds22 - fission yeast (Schizosaccharomyces pombe)

>SWISΞPROT:SD22_SCHPO PROTEIN PHOSPHATASES PPl REGULATORY SUBUNIT

SDS22. >TREMBL:SPAC4A8_12 gene: "sds22"; product: "phosphatases ppl regulatory subunit"; S. pombe chromosome I cosmid c4A8.

Score = 208, P = 8.5e-17, identities = 52/127, positives = 71/127

Entry CEK10D2_5 from database TREMBL: gene: "K10D2.1"; Caenorhabditis elegans cosmid K10D2.

Score = 214, P = 3.6e-16, identities = 50/125, positives = 75/125

Alert BLASTP hits for DKFZphutel_20mll, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphutel_20mll, frame 1

Report for DKFZphutel_20mll .1

[LENGTH] 225

[MW] 25955

[pi] 4.63

[HOMOL] PIR:S 68209 sds22 protein homolog - human le-18

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YKL193c] 2e-ll

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKL193c] 2e-ll

[FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, famesylation and processing) [S. cerevisiae, YKL193c] 2e-ll

[FUNCAT] 30.05 organization of centrosome [S. cerevisiae, YOR373w] 2e-06

[FUNCAT] 01.03 .10 metabolism of cyclic and unusual nucleotides [S. cerevisiae,

YJL005w] 3e-05

[FUNCAT] 03.10 sporulation and germination [S cerevisiae, YJL005w] 3e-05

[FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YJL005w] 3e-05

[FUNCAT] 10.04.03 second messenger formation [S. cerevisiae, YJL005w] 3e-05

[FUNCAT] 04.07 rna transport [S. cerevisiae, YPL169c] 9e-04

[ FUNCAT ] 04.05.01.04 transcriptional control [S. cerevisiae, YCR065w] 9e-04

[EC] 4.6.1.1 Adenylate cyclase 2e-06

[PIRKW] nucleus 5e-16

[PIRKW] duplication 2e-06

[PIRKW] tandem repeat 2e-06

[PIRKW] cAMP biosynthesis 2e-06

[PIRKW] glycoprotein 2e-06

[PIRKW] phosphorus-oxygen lyase 2e-06

[SUPFAM] leucine-rich alpha-2-glycoprotein repeat homology 5e-16

[SUPFAM] fibromodulin 3e-07

[SUPFAM] yeast adenylate cyclase catalytic domain homology 2e-06

[SUPFAM] yeast adenylate cyclase 2e-06

[PROSITE] CK2_PHOSPHO_SITE 2

[PROSITE] PKC PHOSPHO SITE 1 [KW] All_Alpha

SEQ MNQPCNSMEPRVMDDDMLKLAVGDQGPQEEAGQLAKQEGILFKDVLSLQLDFRNILRIDN

PRD ccccccccccccccchhhhhhcccccchhhhhhhhhhhchhhhhhhhhcccccccccccc

SEQ LWQFENLRKLQLDNNIIEKIEGLENLAHLVWLDLSFNNIETIEGLDTLVNLEDLSLFNNR

PRD hhhhhhhhhhhhcccccccccccchhhhhhhhcccccccccccccchhhhhhhhhccccc

SEQ ISKIDΞLDALVKLQVLSLGNNRIDNMMNIIYLRRFKCLRTLSLSRNPISEAEDYKMFICA

PRD cccchhhhhhhhhhhhhccccccccccccccchhhhhhhhhcccccccccchhhhhhhhh

SEQ YLPDLMYLDYRRIDDHTASVSLSVSQPCETDSSSPQVSWKRGIEE

PRD hhcccccccccccccchhhhhhhhccccccccccccccccccccc

Prosite for DKFZphutel_20mll .1

PS00005 218->221 PKC_PHOSPHO_SITE PDOC00005 PS00006 122->126 CK2_PHOSPHO_SITE PDOC00006 PS00006 169->173 CK2 PHOSPHO SITE PDOC00006

(No Pfam data available for DKFZphutel_20mll .1)

DKFZphutel_20m24 group: metabolism

DKFZphutel_20m24 encodes a novel 611 amino acid protein with similarity to a hypothetical C. elegans protein and to yeast Alg9 protein.

This protein is a putative mannosyl transferase that is involved in the assembly of the core oligosaccharide Glc3Man9GlcNAc2.

The new protein can find application in modulation of glycosylation of proteins and as a new enzyme for biotechnologic production processes. strong similarity to S. cerevisiae Alg9p complete cDNA, complete eds, potential start at Bp 23, few EST hits

Alg9 is involved in the assembly of the core oligosaccharide

Glc3Man9GlcNAc2

HSAC381 corresponding genomic DNA (2 exons)

HSB8954 corresponding genomic DNA (1 exon )

Sequenced by AGOWA

Locus: /map="ll"

Insert length: 1986 bp

Poly A stretch at pos. 1966, polyadenylation signal at pos. 1949

1 TTCTTTTTTC CCCAGGCTTG CCATGGCTAG TCGAGGGGCT CGGCAGCGCC 51 TGAAGGGCAG CGGGGCCAGC AGTGGGGATA CGGCCCCGGC TGCGGACAAG

101 CTGCGGGAGC TGCTGGGCAG CCGAGAGGCG GGCGGCGCGG AGCACCGGAC

151 CGAGTTATCT GGGAACAAAG CAGGACAAGT CTGGGCACCT GAAGGATCTA

201 CTGCTTTCAA GTGTCTGCTT TCAGCAAGGT TATGTGCTGC TCTCCTGAGC

251 AACATCTCTG ACTGTGATGA AACATTCAAC TACTGGGAGC CAACACACTA

301 CCTCATCTAT GGGGAAGGGT TTCAGACTTG GGAATATTCC CCAGCATATG

351 CCATTCGCTC CTATGCTTAC CTGTTGCTTC ATGCCTGGCC AGCTGCATTT

401 CATGCAAGAA TTCTACAAAC TAATAAGATT CTTGTGTTTT ACTTTTTGCG

451 ATGTCTTCTG GCTTTTGTGA GCTGTATTTG TGAACTTTAC TTTTACAAGG

501 CTGTGTGCAA GAAGTTTGGG TTGCACGTGA GTCGAATGAT GCTAGCCTTC

551 TTGGTTCTCA GCACTGGCAT GTTTTGCTCA TCATCAGCAT TCCTTCCTAG

601 TAGCTTCTGT ATGTACACTA CGTTGATAGC CATGACTGGA TGGTATATGG

651 ACAAGACTTC CATTGCTGTG CTGGGAGTAG CAGCTGGGGC TATCTTAGGC

701 TGGCCATTCA GTGCAGCTCT TGGTTTACCC ATTGCCTTTG ATTTGCTGGT

751 CATGAAACAC AGGTGGAAGA GTTTCTTTCA TTGGTCGCTG ATGGCCCTCA

801 TACTATTTCT GGTGCCTGTG GTGGTCATTG ACAGCTACTA TTATGGGAAG

851 TTGGTGATTG CACCACTCAA CATTGTTTTG TATAATGTCT TTACTCCTCA

901 TGGACCTGAT CTTTATGGTA CAGAACCCTG GTATTTCTAT TTAATTAATG

951 GATTTCTGAA TTTCAATGTA GCCTTTGCTT TGGCTCTCCT AGTCCTACCA 1001 CTGACTTCTC TTATGGAATA CCTGCTGCAG AGATTTCATG TTCAGAATTT 1051 AGGCCACCCG TATTGGCTTA CCTTGGCTCC AATGTATATT TGGTTTATAA 1101 TTTTCTTCAT CCAGCCTCAC AAAGAGGAGA GATTTCTTTT CCCTGTGTAT 1151 CCACTTATAT GTCTCTGTGG CGCTGTGGCT CTCTCTGCAC TTCAGAAATG 1201 TTACCACTTT GTGTTTCAAC GATATCGCCT GGAGCACTAT ACTGTGACAT 1251 CGAATTGGCT GGCATTAGGA ACTGTCTTCC TGTTTGGGCT CTTGTCATTT 1301 TCTCGCTCTG TGGCACTGTT CAGAGGATAT CACGGGCCCC TTGATTTGTA 1351 TCCAGAATTT TACCGAATTG CTACAGACCC AACCATCCAC ACTGTCCCAG 1401 AAGGCAGACC TGTGAATGTC TGTGTGGGAA AAGAGTGGTA TCGATTTCCC 1451 AGCAGCTTCC TTCTTCCTGA CAATTGGCAG CTTCAGTTCA TTCCATCAGA 1501 GTTCAGAGGT CAGTTACCAA AACCTTTTGC AGAAGGACCT CTGGCCACCC 1551 GGATTGTTCC TACTGACATG AATGACCAGA ATCTAGAAGA GCCATCCAGA 1601 TATATTGATA TCAGTAAATG CCATTATTTA GTGGATTTGG ACACCATGAG 1651 AGAAACACCC CGGGAGCCAA AATATTCATC CAATAAAGAA GAATGGATCA 1701 GCTTGGCCTA TAGACCATTC CTTGATGCTT CTAGATCTTC AAAGCTGCTG 1751 CGGGCATTCT ATGTCCCCTT CCTGTCAGAT CAGTATACAG TGTACGTAAA 1801 CTACACCATC CTCAAACCCC GGAAAGCAAA GCAAATCAGG AAGAAAAGTG 1851 GAGGTTAGCA ACACACCTGT GGCCCCAAAG GACAACCATC TTGTTAACTA 1901 TTGATTCCAG TGACCTGACT CCCTGCAAGT CATCGCCTGT AACATTTGTA 1951 ATAAAGGTCT TCTGACATGA AAAAAAAAAA AAAAAA

BLAST Results

Entry HSAC381 from database EMBL:

Homo sapiens chromosome 11 pac pDJ159ol, complete sequence.

Length = 42,771

Entry HSB8954 from database EMBL: cSRL-50A3-u cSRL flow sorted Chromosome 11 specific cosmid Homo sapiens genomic clone CSRL-50A3. Length = 601

Medl e entries

96293493:

Stepwise assembly of the lipid-l nked oligosaccharide in the endoplasmic reticulum of Saccharomyces cerevisiae: identification of the ALG9 gene encoding a putative mannosyl transferase.

Peptide information for frame 2

ORF from 23 bp to 1855 bp; peptide length: 611 Category: strong similarity to known protein

1 MASRGARQRL KGSGASSGDT APAADKLREL LGSREAGGAE HRTELSGNKA

51 GQVWAPEGST AFKCLLSARL CAALLSNIΞD CDETFNYWEP THYLIYGEGF

101 QTWEYSPAYA IRSYAYLLLH AWPAAFHARI LQTNKILVFY FLRCLLAFVS

151 CICELYFYKA VCKKFGLHVS RMMLAFLVLS TGMFCSSSAF LPSSFCMYTT

201 LIAMTGWYMD KTSIAVLGVA AGAILGWPFS AALGLPIAFD LLVMKHRWKS

251 FFHWSLMALI LFLVPVVVID SYYYGKLVIA PLNIVLYNVF TPHGPDLYGT

301 EPWYFYLING FLNFNVAFAL ALLVLPLTSL MEYLLQRFHV QNLGHPYWLT

351 LAPMYIWFII FFIQPHKEER FLFPVYPLIC LCGAVALSAL QKCYHFVFQR

401 YRLEHYTVTS NWLALGTVFL FGLLSFSRSV ALFRGYHGPL DLYPEFYRIA

451 TDPTIHTVPE GRPVNVCVGK EWYRFPSSFL LPDNWQLQFI PSEFRGQLPK

501 PFAEGPLATR IVPTDMNDQN LEEPSRYIDI SKCHYLVDLD TMRETPREPK

551 YSSNKEEWIS LAYRPFLDAS RSSKLLRAFY VPFLSDQYTV YVNYTILKPR

601 KAKQIRKKSG G

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_20m24, frame 2

SWISSPROT :YTH3_CAEEL HYPOTHETICAL 75.5 KD PROTEIN C14A4.3 IN CHROMOSOME II., N = 1, Score = 957, P = 2.7e-96

PIR:Ξ63177 mannosyl transferase (EC 2.4.1.-) - yeast (Saccharomyces cerevisiae), N = 1, Score = 533, P = 2.3e-51

SWISSPROT :YTH3_CAEEL HYPOTHETICAL 75.5 KD PROTEIN C14A4.3 IN CHROMOSOME II., N = 1, Score = 957, P = 2.7e-96

PIR:S63177 mannosyl transferase (EC 2.4.1.-) - yeast (Saccharomyces cerevisiae), N = 1, Score = 533, P = 2.3e-51

>SWISSPROT:YTH3_CAEEL HYPOTHETICAL 75.5 KD PROTEIN C14A4.3 IN CHROMOSOME II.

Length = 653

HSPs:

Score = 957 (143.6 bits), Expect = 2.7e-96, P = 2.7e-96 Identities = 206/514 (40%), Positives = 296/514 (57%)

Query: 48 NKAGQVWAPEGSTAFKCLLSARLCAALLSNISDCDETFNYWEPTHYLIYGEGFQTWEYSP 107

N W + FK LLS R+ A+ I+DCDE +NYWEP H +YGEGFQTWEYSP Sbjct: 43 NNPDNDWPFSFGSVFKMLLSIRISGAIWGIINDCDEVYNYWEPLHLFLYGEGFQTWEYSP 102

Query: 108 AYAIRSYAYLLLHAWPAAFHARILQTNKILVFYFLRCLLAFVSCICELYFYKAVCKKFGL 167

YAIRSY Y+ LH PA+ A + KI+VF +R + + E Y + A+CKK + Sbjct: 103 VYAIRSYFYIYLHYIPASLFANLFGDTKIVVFTLIRLTIGLFCLLGEYYAFDAICKKINI 162

Query: 168 HVSRMMLAFLVLSTGMFCSSSAFLPSSFCMYTTLIAMTGWYMDKTSIAVLGVAAGAILGW 227

R + F + S+GMF +S+AF+PSSFCM T + + + + + VA ++GW Sbjct: 163 ATGRFFILFSIFSSGMFLASTAFVPSSFCMAITFYILGAYLNENWTAGIFCVAFSTMVGW 222

Query: 228 PFSAALGLPIAFDLLVMKHRWKSFFHWSLMALILFLVPVVVIDSYYYGKLVIAPLNIVLY 287 PFSA LGLPI D+L++K F SL+ + V+ DS+Y+GK V+APLNI LY Sbjct: 223 PFSAVLGLPIVADMLLLKGLRIRFILTSLVIGLCIGGVQVITDSHYFGKTVLAPLNIFLY 282

Query: 288 NVFTPHGPDLYGTEPWYFYLINGFLNFNVAFALALLVLPLTSLMEYLLQRFHVQNLGHPY 347

NV + GP LYG EP FY+ N F N+N+ A PL+ + Y + + Q+ Sbjct: 283 NVVSGPGPSLYGEEPLSFYIKNLFNNWNIVIFAAPFGFPLS--LAYFTKVWMΞQDRNVAL 340

Query: 348 WLTLAPMYI WFIIFFIQPHKEERFLFPVYPLICLCGAVALSALQKCYHFVFQR 400

+ AP+ + W +IF Q HKEERFLFP+YP I A+AL A + ++ Sbjct: 341 YQRFAPIILLAVTTAAWLLIFGSQAHKEERFLFPIYPFIAFFAALALDATNR LCLKK 397

Query: 401 YRLEHYTVTSNWLALGTVFLFGLLSFSRSVALFRGYHGPLDLYPEFYRIATDPTIHTVPE 460

++ N L++ + F +LS SR+ ++ Y +++Y T+ T + Sbjct: 398 LGMD NILSILFILCFAILSAΞRTYSIHNNYGSHVEIYRSLNAELTNRT-NFKNF 450

Query: 461 GRPVNVCVGKEWYRFPSSFLLPDNW QLQFIPSEFRGQLPKPFAEGPL ATRI 511

P+ VCVGKEW+RFPSSF +P +++FI SEFRG LPKPF + TR

Sbjct: 451 HDPIRVCVGKEWHRFPSSFFIPQTVSDGKKVEMRFIQSEFRGLLPKPFLKSDKLVEVTRH 510

Query: 512 VPTDMNDQNLEEPSRYIDIΞKCHYLVDLDTMRETPREPKYSSNKEEW 558

+PT+MN+ N EE SRY+D+ C Y+VD+D M ++ REP + ++ + Sbjct: 511 IPTEMNNLNQEEISRYVDLDSCDYVVDVD-MPQSDREPDFRKMRQNY 556

Pedant information for DKFZphutel_20m24, frame 2

Report for DKFZphutel_20m24.2

[LENGTH] 611

[MW] 69863.78

[pi] 8.91

[HOMOL] SWISSPROT :YTH3_CAEEL HYPOTHETICAL 75.5 KD PROTEIN C14A4.3 IN CHROMOSOME II. 2e-

93

[FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YNL219c] 4e-69

[FUNCAT] 01.06.01 lipid, fatty-acid and sterol biosynthesis [S. cerevisiae, YNL219c]

4e-69

[FUNCAT] 01.05.01 carbohydrate utilization [S. cerevisiae, YNL219c] 4e-69

[PIRKW] glycosyltransferase 9e-68

[PIRKW] transmembrane protein 9e-68

[PIRKW] hexosyltransferase 9e-68

[PROSITE] MYRISTYL 9

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 7

[PROSITE] PKC_PHOSPHO_SITE 6

[PROSITE] ASN_GLYCOSYLATION 2

[KW] TRANSMEMBRANE 7

[KW] LOW COMPLEXITY 6.71 %

SEQ MASRGARQRLKGSGASSGDTAPAADKLRELLGSREAGGAEHRTELΞGNKAGQVWAPEGST SEG PRD ccchhhhhhhcccccccccccchhhhhhhhhccccccccccceeecccccccccccccch MEM MMMMMM

SEQ AFKCLLSARLCAALLΞNISDCDETFNYWEPTHYLIYGEGFQTWEYSPAYAIRSYAYLLLH SEG ... xxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhccccceeeccccceeeeeccccceeecccchhhhhhhhhhhc MEM MMMMMMMMMMMMMMMMM M

SEQ AWPAAFHARILQTNKILVFYFLRCLLAFVSCICELYFYKAVCKKFGLHVSRMMLAFLVLS SEG PRD cchhhhhhhhhcchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ TGMFCSSSAFLPSSFCMYTTLIAMTGWYMDKTSIAVLGVAAGAILGWPFSAALGLPIAFD

SEG xxxxxxxxxxxxx

PRD cceeeeccccccchhhhhhhhhhhhcccccccceeeeeehhhhhhccceeeeeecchhhh

MEM MMMMMMMMMMMMMM

SEQ LLVMKHRWKSFFHWSLMALILFLVPVVVIDSYYYGKLVIAPLNIVLYNVFTPHGPDLYGT SEG PRD hhhhhhhhhhhhhhhhhhhhhheeeeeeeecccccccccccceeeeeeeecccccccccc MEM MMMMMMM. MMMMMMMMMMMMMMMMMMMMM

SEQ EPWYFYLINGFLNFNVAFALALLVLPLTΞLMEYLLQRFHVQNLGHPYWLTLAPMYIWFII

SEG xxxxxxxxxxxxxxx

PRD cceeeeeecccccchhhhhhhhhhhhchhhhhhhhhhhhccccccceeeeehhhhhhhhh

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ FFIQPHKEERFLFPVYPLICLCGAVALΞALQKCYHFVFQRYRLEHYTVTSNWLALGTVFL SEG PRD hhcccchhhhhhcccceeehhhhhhhhhhhhhhhhhhhhhhhhheeeeccchhhhhhhee MEM MMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMM .

SEQ FGLLSFSRSVALFRGYHGPLDLYPEFYRIATDPTIHTVPEGRPVNVCVGKEWYRFPSSFL SEG PRD eehhhhhhhheeecccccccccccceeeeccccccceeecccceeeeeeccccccccccc MEM

SEQ LPDNWQLQFIPSEFRGQLPKPFAEGPLATRIVPTDMNDQNLEEPSRYIDISKCHYLVDLD SEG PRD ccccceeeecccccccccccccccccceeeeccccccccccccccceeeeeeceeeeecc

MEM

SEQ TMRETPREPKYSSNKEEWISLAYRPFLDASRSSKLLRAFYVPFLSDQYTVYVNYTILKPR SEG PRD cccccccccccchhhhhhhhhhhhhhhhhhhhhhheeeeeeeeecceeeeeeeeeecccc

MEM

SEQ KAKQIRKKSGG SEG PRD hhhhhhccccc MEM

Prosite for DKFZphutel_20m2 .2

PS00001 77->81 ASN_GLYCOSYLATION PDOC00001 PS00001 593->597 ASN_GLYCOSYLATION PDOC00001 PS00004 606->610 CAMP_PHOSPHO_SITE PDOC00004 PS00005 67->70 PKC_PHOSPHO_SITE PDOC00005 PS00005 133->136 PKC_PHOSPHO_SITE PDOC00005 PS00005 541->544 PKC_PHOSPHO_SITE PDOC00005 PS00005 545->548 PKC_PHOSPHO_SITE PDOC00005 PS00005 553->556 PKC_PHOSPHO_SITE PDOC00005 PS00005 572->575 PKC_PHOSPHO_SITE PDOC00005 PS00006 16->20 CK2_PHOSPHO_SITE PDOC00006 PS00006 79->83 CK2_PHOSPHO_SITE PDOC00006 PS00006 329->333 CK2_PHOSPHO_SITE PDOC00006 PS00006 457->461 CK2_PHOSPHO_SITE PDOC00006 PS00006 541->545 CK2_PHOSPHO_SITE PDOC00006 PS00006 545->549 CK2_PHOSPHO_SITE PDOC00006 PS00006 553->557 CK2_PHOSPHO_SITE PDOC00006 PS00008 12->18 MYRISTYL PDOC00008 PS00008 14->20 MYRISTYL PDOC00008 PS00008 32->38 MYRISTYL PDOC00008 PS00008 47->53 MYRISTYL PDOC00008 PS00008 166->172 MYRISTYL PDOC00008 PS00008 182-M88 MYRISTYL PDOC00008 PS00008 218->224 MYRISTYL PDOC00008 PS00008 222->228 MYRISTYL PDOC00008 PΞ00008 234->240 MYRISTYL PDOC00008

(No Pfam data available for DKFZphutel 20m24.2)

DKFZphutel_21dl5

group: uterus derived

DKFZphutel_21dl5 encodes a novel 191 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application m studying the expression profile of testis-specific genes . unknown

Sequenced by MediGenomix

Locus: /chromosome="3"

Insert length: 5292 bp

Poly A stretch at pos. 5273, polyadenylation signal at pos. 5252

1 CTCCCACTAG TGTATGCCTT AATGGTGCCG CTCTTGTCCG CGTCTACGCT 51 TGGGACCTTG GCTTCTGACT TGGAGAGTGT ACAGCTCTGC CCGACGGCAA

101 CCCAGCTTGG GAAGAGAAGC CCCAGCGTGG GCTGGGGCTC AAGGCGCAGG

151 AAGGCCGAGC CCGGCGCGGA CGCAGGCGGC TCCGGGCGGG CTCAGCACCC

201 CCAGGCACCG TCTCCTAGTG ACCGCGGCGC TCGCGGGCCT GGCGGCCGTT

251 GTCCGGGCGA CTGCGCAGCG CGGGCACCCC CGCGGCCCCT CCCCTGGGCG

301 CGCGCGCGAC CTGGGTGCCA TGGCGGCAGC GGCGGTGACA GGCCAGCGGC

351 CTGAGACCGC GGCGGCCGAG GAGGCCTCGA GGCCGCAGTG GGCGCCGCCA

401 GACCACTGCC AGGCTCAGGC GGCGGCCGGG CTGGGCGACG GCGAGGACGC

451 ACCGGTGCGT CCGCTGTGCA AGCCCCGCGG CATCTGCTCG CGCGCCTACT

501 TCCTGGTGCT GATGGTGTTC GTGCACCTGT ACCTGGGTAA CGTGCTGGCG

551 CTGCTGCTCT TCGTGCACTA CAGCAACGGC GACGAAAGCA GCGATCCCGG

601 GCCCCAACAC CGTGCCCAGG GCCCCGGGCC CGAGCCCACC TTAGGTCCCC

651 TCACCCGGCT GGAGGGCATC AAGGTGAGGA CCTCCCTGCC CCGCCGCGCT

701 CCAGGCCCTG CACGGCTGAG CCCGAGAGGA CCGGCGCTCA GCCCGGGTCC

751 CCACGCTGCC CCCGGCGCTG CTCTGCGTCG GTCCCGCGCG CTCCCACTCA

801 CTCGCCTGCT GTCGCTCTCC GGGCCGGGGC GACTTGGCCC TTTTTGGGCA

851 GCGCGGTCTG GCGCCCCAGC TGCCCGCTGT GCGCCTTTTC CTTAGGTGGG

901 GCACGAGCGT AAGGTCCAGC TGGTCACCGA CAGGGATCAC TTCATCCGAA

951 CCCTCAGCCT CAAGCCGCTG CTCTTCGAAA TCCCCGGCTT CCTGACTGAT

1001 GAAGAGTGTC GGCTCATCAT CCATCTGGCG CAGATGAAGG GGTTACAGCG

1051 CAGCCAGATC CTGCCTACTG AAGAGTATGA AGAGGCAATG AGCACTATGC

1101 AGGTCAGCCA GCTGGACCTC TTCCGGCTGC TGGACCAGAA CCGTGATGGG

1151 CACCTTCAGC TCCGTGAGGT TCTGGCCCAG ACTCGCCTGG GAAATGGATG

1201 GTGGATGACT CCAGAGAGCA TTCAGGAGAT GTACGCCGCG ATCAAGGCTG

1251 ACCCTGATGG TGACGGTGAG CTCACACCTC TGCACAGTCC TATCCCCGTG

1301 AGCCTCCTGC CCACTCCCAG GTGCACAATT TTGAAAACTT GGGCCCTTCC

1351 CCCACAGCCA GGCAGCCTCT CTGCACCCCT TTATAGTGGC CAGAGATGGG

1401 GAGGTGAAGA TCCAGCCTTG CTTTTTACCC CTGGGAAGTA GGCAGGCAGC

1451 CAGGCCCCCC GTTCCCCTTG GTGATGGTCT CGAGGGCAGT TCTTGGAGAC

1501 CCTTTTGATA ACATCAGGCA GAGTTGAGAG CCTGGGGACA GGAAGTAGGG

1551 CTGCTAGTTG GCAGAGAACA GAGTGGGTGG AGCAGGAGCA AGGCGACAGT

1601 GAGGCCAGCT AGAGCTTGGC TGTTTACCCT GCTCCATCCA TCTCTCCAGC

1651 CAGACACGAG GTCCACCCCA GCAGACAGCT TCCCTGGTCT AAGTGAGGTC

1701 TCCCTTGCCT TCCTCTTGTC CACCTGGAGT CATGCCGAAG CGCCTAAAAT

1751 GGTAGTGCTG CTACCTGTGC TAACTGCTGG GGAGGGGTGG GCAGGGAAGC

1801 TGTCATGCAA GTGGTGCCCC CTCTGGTAAT AACTCTCAGG AGGTTTCTGA

1851 GGTGTGGTCA TCACCCTCAT GCCCAAATTC TGGACCAAGA GAGGAAGATA

1901 CAGCAGTTAG AAAGGACTTG GAACAGTGGC TTTGCGGCTG GTGAACCAGA

1951 GTGAAGAATC TGGCCGTGAC CTGGCTGCCA CACTGCTATA GGCCCCAGAA

2001 CAGAGGTGGT GACAGTCTCA CAGCCCTTGA ATGTCCCCCA CCCTCAGAGG

2051 AATCTGGGCC AAAGAGTGGA AGGTGATGTC CTTGGGTCAG CCAGAATAAC

2101 ATGGAGCAAA GATACCAACT ACTCTTCCAG AACCCCAAGA GGGTAGAACC

2151 CCTGCTTAAT GGTTTGAGCA GGGACAGTGG AGAATGTTCT CATGAGAGGG

2201 GGTGGCCTGA CTTTCGTTGC TAAGTGGGCT GGTAACGCAG TAGGCAGGGC

2251 TGGCGAAGTA GGTTCCACCC AGGATGAAAC CTGGGGTCAT GAGGAACTCC

2301 CCGGGGGCTG GCCCTGCTTG CACCCTGGCG TATGTATGTA AGGCCCTGGA

2351 TGAGGCCCAG CACTGCCTGC TCTCTCCTCA CCCTCCACAG GCCGGAGAGT

2401 GGCCACCACT CTATATAGCC AGGCTGGAAG GCCAGGGTCC TGGCCATATG

2451 GCTCAAGCTT CCTTTGGAGA ACCTTCTCTG GCCACTCTAA TAGGGGGTGG

2501 GCCTCTTTCT TCTTAGGGCC AAATTAGGGC TTAAACTGAG AAAAGGAACT

2551 GCTCTGGGTC TTCCTGTAAG GCCTGATGTG ACAGAAACCA GGTTCATCTG

2601 ACCCAAAAGT CCAGGTGGGG GACAAGTGTA CAAGGCCCCT CAGTGCCTGA

2651 GGTCAGGGGC TGCTGCTGCC TTTGGGGTAG GTAGGGAAGT GCAGCCTGCC

2701 ACTGTTGCCT CCCAATATGG GCTTGGTGGG CATTGATGGT GGGTGCCCTG

2751 TGCAGGAGTG CTGAGTCTGC AGGAGTTCTC CAACATGGAC CTTCGGGACT

2801 TCCACAAGTA CATGAGGAGC CACAAGGCAG AGTCCAGTGA GCTGGTGCGG 2851 AACAGCCACC ATACCTGGCT CTACCAGGGT GAGGGTGCCC ACCACATCAT 2901 GCGTGCCATC CGCCAGAGGT GAGCACCTGA AGCTGTTCTC ACTGGAGCAG 2951 GGGGAGAAGA CTGGGCAGGG CCTCCACAGA AGTCCTTGTC TGGGGCCAAG 3001 AGGACAGAAT GGATTAACCC ATTTGGGATT AAGTTCCATT TGTTAGACCA 3051 GGATTGGGAC CCACTGAAAG ACAGGCAATT AACAAAGGCA AATTAGCCCT 3101 CCTTGCAGGC ACACAATGGG CAACTGGGGT TAGATAGAGA TTGAGCACTT 3151 CTTTCTGATT AGATAAATGA CCTCTTATCT TTGACCCCTT ATCTGACCCC 3201 GTCACAGCAG GAAAAGGGTT TTTAAATAAA CAACTTTCTT CCAGGGAGGA 3251 GGACCTCAGG ACTCCCCGCC CCCTTTATTT AGTGGAAATG TCAACATTTC 3301 CACATAGCAG GTGTCTCTGT CTTTGGCATC TGAGGGAGAA GGATCATCAT 3351 GAGTAACCCC CTCCTGCTCT TACAGGGCCA GTCTGAGATG GCTTAAGGGA 3401 CTTCCAGGGG AGGTGGGTAG GGGCAAAGCT TGTGGCAGGC CTAGGGTCCA 3451 CCTTGGCCAG CTCCTTCAGA TCACCACCTT GCCTGGGGCT GCCCAGCCAA 3501 ATGCCTGCTG CCCACCAGGG TGCTGCGCCT CACTCGCCTG TCGCCTGAGA 3551 TCGTGGAGCT CAGCGAGCCG CTGCAGGTTG TTCGATATGG TGAGGGGGGC 3601 CACTACCATG CCCACGTGGA CAGTGGGCCT GTGTACCCAG AGACCATCTG 3651 CTCCCATACC AAGCTGGTAG CCAACGAGTC TGTACCCTTC GAGACCTCCT 3701 GCCGGCAAGT ATCTCCCAAC TGGGGGCTGC CTTCAATCCT CAGACCAGGA 3751 ACACCCATGA CACAGGCACA GCCCTGCACT GTGGGCGTGC CCCTTGGCAT 3801 GGGGCCAGGA GATCACTGGG TTATCCCGGT TAGTGATGCC CTCACCTCTC 3851 CCCACAAGTT GTTTACCCAA TGGCTGGAAA GGGGTGGCTA CTGGTCATCG 3901 TGACCACTGG AGTCAACACA GACTGATGTA CCCACAGACA CCAAAACTTG 3951 CCCCCTGAGT TCTGAAGCAA GGGGCAAGGC TGGGCCCCTA GCTTGTCCTG 4001 CCCATTCCTC CAGGTGTTGA TCTTGATTCC ACTTAGAGAA GCTGAAGCTG 4051 TGCCTCCCTC CCCTGTCAAG CCAGTTCTTT CCTCTTCAGG TGGCTGTTCT 4101 GGCCCAGCCC CTTCCCATCC CCAAGGAGCC CTTCAGCGCG CCCTGTTGCT 4151 TCTGCTAGCC TACCTTTCCC TGCCAGGCCC TTGCTCAGGG CCATGGCATT 4201 TAACTAAGTG CACCTGTGAT CTTGGCCAAA AAACCATTGC AACTCACAGT 4251 AAGAGACTGG GTTTCGGGGA AGGAGGGGCT AGGGACATTT TGGCACTGGC 4301 CTGCCCTATT GTCTCCCATC CTAGTCTGTC CTGGTCCCTG GCAACAGGAA 4351 CCTGGGCAGC TTATCCTGCC CACAGGTAAG CCCCTGGGAG CATCCACAAC 4401 TGGGGACCTG CTCAGTGCCC CCCCTGCCTT ACAGCTACAT GACAGTGCTG 4451 TTTTATTTGA ACAACGTCAC TGGTGGGGGC GAGACTGTTT TCCCTGTAGC 4501 AGATAACAGA ACCTACGATG AAATGGTAAG GGTCAACTGG GCTATTACTC 4551 TTGTGGGCTG GCAGGGGCTT AGACAAGTGA AGTACACACC TCTCCAGGTC 4601 TAAGGATGTG GGCCCAAATT ATTCCTTGGG CATATCTGGT TGGTTTCCCT 4651 TTGGTCACCC TTGGCTGGCC TGGCCATAGA GTGGGGACAG GTTGAACACC 4701 CCACCACCCT GCTGCCCACA GAGTCTGATT CAGGATGACG TGGACCTCCG 4751 TGACACACGG AGGCACTGTG ACAAGGGAAA CCTGCGTGTC AAGCCCCAAC 4801 AGGGCACAGC AGTCTTCTGG TACAACTACC TGCCTGATGG GCAAGGTTGG 4851 GTGGGTGACG TAGACGACTA CTCGCTGCAC GGGGGCTGCC TGGTCACGCG 4901 CGGCACCAAG TGGATTGCCA ACAACTGGAT TAATGTGGAC CCCAGCCGAG 4951 CGCGGCAAGC GCTGTTCCAA CAGGAGATGG CCCGCCTTGC CCGAGAAGGG 5001 GGCACCGACT CACAGCCCGA GTGGGCTCTG GACCGGGCCT ACCGCGATGC 5051 GCGCGTGGAA CTCTGAGGGA AGAGTTAGCC CCGGTTCCCA GCCGCGGGTC 5101 GCCAGTTGCC CAAGATCAGG GGTCCGGCTG TCCTTCTGTC CTGCTGCAGA 5151 CTAAAGGTCT GGCCAATGTC TTGCCCCACC CCGCCAGCCG CGATACGGCG 5201 CAGTTCCTAT ATTCATGTTA TTTATTGTGT ACTGACTCCA TCTGCCCCGT 5251 CAAATAAAAA ACCACAAGGT TCGAAAAAAA AAAAAAAAAA GG

BLAST Results

Entry HSU64252 from database EMBL:

Human STS sequence NOTI-225.

Score = 959, P = 1.2e-36, identities = 195/199

Medline entries

No Medline entry

Peptide information for frame 1

ORF from the beginning to 351 bp; peptide length: 118 Category: questionable ORF Classification: no clue

1 LPLVYALMVP LLSASTLGTL AΞDLESVQLC PTATQLGKRS PSVGWGSRRR 51 KAEPGADAGG ΞGRAQHPQAP SPSDRGARGP GGRCPGDCAA RAPPRPLPWA 101 RARPGCHGGS GGDRPAA

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphutel_21dl5, frame 1 No Alert BLASTP hits found

Peptide information for frame 2

ORF from 320 bp to 892 bp; peptide length: 191 Category: putative protein Classification: no clue

1 MAAAAVTGQR PETAAAEEAS RPQWAPPDHC QAQAAAGLGD GEDAPVRPLC

51 KPRGICSRAY FLVLMVFVHL YLGNVLALLL FVHYSNGDES SDPGPQHRAQ

101 GPGPEPTLGP LTRLEGIKVR TSLPRRAPGP ARLSPRGPAL SPGPHAAPGA

151 ALRRSRALPL TRLLSLSGPG RLGPFWAARS GAPAARCAPF P

BLASTP hits

No BLASTP h ts available

Alert BLASTP hits for DKFZphutel_21dl5, frame 2

PIR:EDBE75 immediate-early protein IE175 - human herpesvirus 1, N = 2, Score = 106, P = 0.0067

>PIR:EDBE75 immediate-early protein IE175 - human herpesvirus 1 Length = 1,298

HSPs:

Score = 106 (15.9 bits), Expect = 6.7e-03, Sum P(2) = 6.7e-03 Identities = 36/103 (34%), Positives = 44/103 (42%)

Query 87 GDESSDPGPQHRAQGPGPEPTLGPLTRLEGIKVRTSLPRRA-PGPARLS-PRGPALSPGP 144 G + PGP G GP P P T+ G S R P PA S P GP +P Sbjct 726 GRKRKSPGPARPPGGGGPRP PKTKKSGADAPGSDARAPLPAPAPPSTPPGPEPAPAQ 782 Query 145 HAAPGAALRRSRALPLT-RLLSLSGPGRLGPFWAARSGAPAARCAP 189

AAP AA ++R P+ GP LG W + P+ AP Sbjct 783 PAAPRAAAAQARPRPVAVSRRPAEGPDPLGG-WRRQPPGPΞHTAAP 827

Score = 40 (6.0 bits), Expect = 6.7e-03, Sum P(2) = 6.7e-03 Identities = 8/21 (38%), Positives = 9/21 (42%)

Query: 28 DHCQAQAAAGLGDGEDAPVRP 48

DH + A G G AP P Sbjct: 212 DHAREARAVGRGPSSAAPAAP 232

Pedant information for DKFZphutel_21dl5, frame 1 Report for DKFZphutel_21dl5.1

[LENGTH] 117

[MW] 11797.32

[pi] 10.68

[KW] Irregular

[KW] SIGNAL PEPTIDE 22

[KW] LOW COMPLEXITY 38.46 %

SEQ LPLVYALMVPLLSASTLGTLASDLESVQLCPTATQLGKRΞPSVGWGSRRRKAEPGADAGG

SEG xxxxxxxxxxxxxx

PRD cccccccccccccccccccchhhhhhhhcccccccccccccccccccccccccccccccc

SEQ SGRAQHPQAPSPSDRGARGPGGRCPGDCAARAPPRPLPWARARPGCHGGSGGDRPAA

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD ccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

(No Prosite data available for DKFZphutel_21dl5.1) (No Pfam data available for DKFZphutel_21dl5.1)

Pedant information for DKFZphutel_21dl5, frame 2

Report for DKFZphutel_21dl5.2

[LENGTH] 191

[MW] 19916.88

[pi] 10.43

[KW] TRANSMEMBRANE 1

[KW] LOW_COMPLEXITY 29.84 %

SEQ MAAAAVTGQRPETAAAEEASRPQWAPPDHCQAQAAAGLGDGEDAPVRPLCKPRGICSRAY

SEG

PRD ccceeeeccccchhhhhhhhhccccccchhhhhhhhcccccccccccccccccccchhhh

MEM

SEQ FLVLMVFVHLYLGNVLALLLFVHYSNGDESSDPGPQHRAQGPGPEPTLGPLTRLEGIKVR

SEG xxxxxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhhccccccccccccccccccccccccccccccccceeeeee

MEM MMMMMMMMMMMMMMMMM

SEQ TSLPRRAPGPARLSPRGPALSPGPHAAPGAALRRSRALPLTRLLSLSGPGRLGPFWAARS

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxx ..xxxx

PRD eeccccccccccccccccccccccccccchhhhhhhcccccceeecccccccchhhhhhc

MEM

SEQ GAPAARCAPFP

SEG xxxxxxxxx ..

PRD ccccccccccc

MEM

(No Prosite data available for DKFZphutel_21dl5.2) (No Pfam data available for DKFZphutel_21dl5.2)

DKFZphutel_22d2

group: signal transduction

DKFZphutel_22d2 encodes a novel 580 amino acid putative GTP-binding protein related to the ras protein. Additionally, the putative protein contains an EF-hand for calcium-bmdmg.

G-protems are involved in various signal transduction pathways, transferring the signal of a cellular receptor to an intracellular signal cascade.

The new protein can find clinical application n modulating/blocking the response to a cellular receptor. similarity to GTP-binding proteins complete cDNA, complete eds, potential start at Bp 64, EST hits complete eds according to K08F11.5 and YAL048c

Sequenced by BMFZ

Locus: /map="17"

Insert length: 3247 bp

Poly A stretch at pos. 3230, no polyadenylation signal found

1 CTCCTGGTGA GAGGAGTCCA CTCCGTGCGT GCGGGCGGAG GCCGGCCCCC

51 GAGAGCCGCC GACATGAAGA AAGACGTGCG GATCCTGCTG GTGGGAGAAC

101 CTAGAGTTGG GAAGACATCA CTGATTATGT CTCTGGTCAG TGAAGAATTT

151 CCAGAAGAGG TTCCTCCCCG GGCAGAAGAA ATCACCATTC CAGCTGATGT

201 CACCCCAGAG AGAGTTCCAA CACACATTGT AGATTACTCA GAAGCAGAAC

251 AGAGTGATGA ACAACTTCAT CAAGAAATAT CTCAGGCTAA TGTCATCTGT

301 ATAGTGTATG CCGTTAACAA CAAGCATTCT ATTGATAAGG TAACAAGTCG

351 ATGGATTCCT CTCATAAATG AAAGAACAGA CAAAGACAGC AGGCTGCCTT

401 TAATATTGGT TGGGAACAAA TCTGATCTGG TGGAATATAG TAGTATGGAG

451 ACCATCCTTC CTATTATGAA CCAGTATACA GAAATAGAAA CCTGTGTGGA

501 GTGTTCAGCG AAAAACCTGA AGAACATATC AGAGCTCTTT TATTACGCAC

551 AGAAAGCTGT TCTTCATCCT ACAGGGCCCC TGTACTGCCC AGAGGAGAAG

601 GAGATGAAAC CAGCTTGTAT AAAAGCCCTT ACTCGTATAT TTAAAATATC

651 TGATCAAGAT AATGATGGTA CTCTCAATGA TGCTGAACTC AACTTCTTTC

701 AGAGGATTTG TTTCAACACT CCATTAGCTC CTCAAGCTCT GGAGGATGTC

751 AAGAATGTAG TCAGAAAACA TATAAGTGAT GGTGTGGCTG ACAGTGGGTT

801 GACCCTGAAA GGTTTTCTCT TTTTACACAC ACTTTTTATC CAGAGAGGGA

851 GACACGAAAC TACTTGGACT GTGCTTCGAC GATTTGGTTA TGATGATGAC

901 CTGGATTTGA CACCTGAATA TTTGTTCCCC CTGCTGAAAA TACCTCCTGA

951 TTGCACTACT GAATTAAATC ATCATGCATA TTTATTTCTC CAAAGCACCT

1001 TTGACAAGCA TGATTTGGAT AGAGACTGTG CTTTGTCACC TGATGAGCTT

1051 AAAGATTTAT TTAAAGTTTT CCCTTACATA CCTTGGGGGC CAGATGTGAA

1101 TAACACAGTT TGTACCAATG AAAGAGGCTG GATAACCTAC CAGGGATTCC

1151 TTTCCCAGTG GACGCTCACG ACTTATTTAG ATGTACAGCG GTGCCTGGAA

1201 TATTTGGGCT ATCTAGGCTA TTCAATATTG ACTGAGCAAG AGTCTCAAGC

1251 TTCAGCTGTT ACAGTGACAA GAGATAAAAA GATAGACCTG CAGAAAAAAC

1301 AAACTCAAAG AAATGTGTTC AGATGTAATG TAATTGGAGT GAAAAACTGT

1351 GGGAAAAGTG GAGTTCTTCA GGCTCTTCTT GGAAGAAACT TAATGAGGCA

1401 GAAGAAAATT CGTGAAGATC ATAAATCCTA CTATGCGATT AACACTGTTT

1451 ATGTATATGG ACAAGAGAAA TACTTGTTGT TGCATGATAT CTCAGAATCG

1501 GAATTTCTAA CTGAAGCTGA AATCATTTGT GATGTTGTAT GCCTGGTATA

1551 TGATGTCAGC AATCCCAAAT CCTTTGAATA CTGTGCCAGG ATTTTTAAGC

1601 AACACTTTAT GGACAGCAGA ATACCTTGCT TAATCGTAGC TGCAAAGTCA

1651 GACCTGCATG AAGTTAAACA AGAATACAGT ATTTCACCTA CTGATTTCTG

1701 CAGGAAACAC AAAATGCCTC CACCACAAGC CTTCACTTGC AATACTGCTG

1751 ATGCCCCCAG TAAGGATATC TTTGTTAAAT TGACAACAAT GGCCATGTAT

1801 CCGTAAGTAC TTGCTGTCTT CATTTTCATG TTGCATGGTT CATAACATTG

1851 CATGCCATTA TTAGCCATGA AGGGAATATC TTTGTCACAT AGGAATTGTT

1901 CAGCAACAGA AAGATACTTT GTAATGAGAA GGTACAAATT TGAGTAAATG

1951 CAAGTTTGGT TTGAATGCCA TAATAAAATG ATATAAACAG TGCTTCTGAC

2001 AATATCTGTA TATTTTTGAG CAGGCTGTAA CTATCTTAAT AGAATAGTAC

2051 AATAAAACAC AACCCCCCAC CCAGCATTAA AAAATAGTTT TACTGGAATA

2101 AAATGGGTTT GGCATCATGT TGTTTTATGC TTATAAAGCA TTTTCATATG

2151 AACAGAAAGT TTATATTTTT CTGTTTTTGA CCTTAGGTAT ATGAAGTTTT

2201 CTAAAATATT TTATTAATTT ATGTTGAAAT TGTGGGTATG CTTCAGTTAG

2251 GATATGTCTT TTTTAAGTGC TGTAAAGAGT AGTTGTAATT GGAATTTCTA

2301 CTGTATAAAT GTTTTACATT AAGTGTTACG AGCCACAAAT TTCATGTACA

2351 TTTATTATAT ATCTATACAT GCATATGCAC AAGCACATAA CTGTGGTCAT

2401 CTCTGTAGTT TACTAACTGC CTTAAAATTG CATGGTTCTT AATGGCATTC

2451 GCCTCAAGTA GTGTGTTTGT ATAAATTCTG TTTTGTAACA AAATAGTTTT

2501 TCAGGCAGTG CGTTTCTCAG GACTTTATAG CTTATTCTAC TTATTCTTAT

2551 GTTAGTCTCT AAATTATTTT TCTTCTTATG AAAACTACAG TGTAACACAG 2601 AGTAATAATC AAACATTGCT ATAAACCAAG AATGACATTT TTCAAAAAGG

2651 TGTTGATTTG TACAGATTTT TAAAGTCAGT TAACTTTACT GCTATTTTAT

2701 TACCTAATAC TTTTTTTAGA TGCAACAAAC CCTTGAATTT CTATTTGTAT

2751 TCGAAGACAA GTCATTCCTA TTATTATAGA ATAACCAAAA CCTTATTTAT

2801 GTTTTACCTT TGCTTTAAAA CTCTCATGTA TGTTATCTAC AGAGAGGATC

2851 ATTACAGAGA CAGACTCTCC CGAGACATGG GCCACACTGA TAGAATAGAG

2901 AATTTGAGAA AAATCTGGGT CTTTCTAAAA ACTGCTTTGT AAGTTACTTT

2951 TTCTTTATGA CTTCTGTGGG ATTTTGTTGA TATTTTCTTA GAGAATGACC

3001 AAATCTCCTT TCTTGCCATA ATTAACATTT AGTAATTATG TAGAAACGCA

3051 CTGCTTGGTC AGGCTTCCTG CCTAGCTATA TATTACGTTG TCTTCCTTAC

3101 TACATAAATG TACTTCTTTA ATCTTGTGAT TACAGTAACT GCAAGTGTGT

3151 TTTTACATCT GCATTTTTAA AACATTTTAC TGTAATTCTG TTGTGTGTGT

3201 GTGTGTTATA TGATAAATGT ACATACATGG AAAAAAAAAA AAAAAAA

BLAST Results

Entry AC004527 from database EMBL:

*** SEQUENCING IN PROGRESS *** NFl-related locus, Direct Submission;

HTGS phase 1, 10 unordered pieces.

Score = 1899, P = l.le-78, identities = 387/396

Entry HS148355 from database EMBL: human STS SHGC-31220. Score = 1826, P = 7.5e-78, identities 388/406

Medline entries

No Medline entry

Peptide information for frame 1

ORF from 64 bp to 1803 bp; peptide length: 580 Category: similarity to known protein

1 MKKDVRILLV GEPRVGKTSL IMΞLVSEEFP EEVPPRAEEI TIPADVTPER 51 VPTHIVDYSE AEQSDEQLHQ EISQANVICI VYAVNNKHSI DKVTSRWIPL 101 INERTDKDSR LPLILVGNKS DLVEYSSMET ILPIMNQYTE IETCVECSAK 151 NLKNISELFY YAQKAVLHPT GPLYCPEEKE MKPACIKALT RIFKISDQDN 201 DGTLNDAELN FFQRICFNTP LAPQALEDVK NVVRKHISDG VADSGLTLKG 251 FLFLHTLFIQ RGRHETTWTV LRRFGYDDDL DLTPEYLFPL LKIPPDCTTE 301 LNHHAYLFLQ STFDKHDLDR DCALSPDELK DLFKVFPYIP WGPDVNNTVC 351 TNERGWITYQ GFLSQWTLTT YLDVQRCLEY LGYLGYSILT EQESQASAVT 401 VTRDKKIDLQ KKQTQRNVFR CNVIGVKNCG KSGVLQALLG RNLMRQKKIR 451 EDHKSYYAIN TVYVYGQEKY LLLHDISESE FLTEAEIICD VVCLVYDVSN 501 PKSFEYCARI FKQHFMDSRI PCLIVAAKSD LHEVKQEYSI SPTDFCRKHK 551 MPPPQAFTCN TADAPΞKDIF VKLTTMAMYP

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_22d2, frame 1

TREMBL :CEUK08F11_3 gene: "K08F11.5"; Caenorhabditis elegans cosmid K08F11., N = 1, Score = 1357, P = l.le-138

TREMBL :SPCC320_4 gene: "SPCC320.04c"; product: "hypothetical protein" S. pombe chromosome III cosmid c320., N = 1, Score = 889, P = 4.4e-89

TREMBL :CEUC47C12_3 gene: "C47C12.4"; Caenorhabditis elegans cosmid C47C12., N = 2, Score = 408, P = 5.6e-74

PIR:S51971 probable membrane protein YAL048c - yeast (Saccharomyces cerevisiae), N = 1, Score = 677, P = 1.3e-66

>TREMBL:CEUK08F11_3 gene: "K08F11.5"; Caenorhabditis elegans cosmid K08F11.

Length = 625

HSPs: Score = 1357 (203.6 bits), Expect = l.le-138, P = l.le-138 Identities = 263/582 (45%), Positives = 380/582 (65%)

Query: 4 DVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQ 63

DVRI+L+G+ GKTSL+MSL+ +E+ + VP R + + IPADVTPE V T IVD S E+ Sbjct: 9 DVRIVLIGDEGCGKTSLVMΞLLEDEWVDAVPRRLDRVLIPADVTPENVTTSIVDLSIKEE 68

Query: 64 SDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLV 123

+ + El QANVIC+VY+V ++ ++D + ++W+PLI + + P+ILVGNKSD Sbjct: 69 DENWIVΞEIRQANVICVVYSVTDESTVDGIQTKWLPLIRQSFGEYHETPVILVGNKSDGT 128

Query: 124 EYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKP 183

++ + ILPIM TE+ETCVECSA+ +KN+SE+FYYAQKAV++PT PLY + K++ Sbjct: 129 A-NNTDKILPIMEANTEVETCVECSARTMKNVSEIFYYAQKAVIYPTRPLYDADTKQLTD 187

Query: 184 ACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHISDGVAD 243

KAL R+FKI D+DNDG L+D ELN FQ++CF PL ALEDVK V DGVA+ Sbjct: 188 RARKALIRVFKICDRDNDGYLSDTELNDFQKLCFGIPLTSTALEDVKRAVSDGCPDGVAN ₂47

Query: 244 SGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNH 303

L L GFL+LH LFI+RGRHETTW VLR+FGY+ L L+ +YL+P + IP C+TEL+ Sbjct: 248 DSLMLAGFLYLHLLFIERGRHETTWAVLRKFGYETSLKLSEDYLYPRITIPVGCSTELΞP 307

Query: 304 HAYLFLQSTFDKHDLDRDCALSPDELKDLFKVFPYIPWGPDVNNTVCTNERGWITYQGFL 363

F+ + F+K+D D+D LSP EL++LF V P D + TN+RGW+TY G++ Sbjct: 308 EGVQFVSALFEKYDEDKDGCLSPSELQNLFSVCPVPVITKDNILALETNQRGWLTYNGYM 367

Query: 364 SQWTLTTYLDVQRCLEYLGYLGYSILTEQESQAS AVTVTRDKKIDLQKKQTQRNVF 419

+ W +TT +++ + E L YLG+ + +A ++ VTR++K DL+ T R VF Sbjct: 368 AYWNMTTLINLTQTFEQLAYLGFPVGRSGPGRAGNTLDSIRVTRERKKDLENHGTDRKVF 427

Query: 420 RCNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDI 476

+C V+G K+ GK+ +Q+L GR + +1 H S + IN V V + KYLLL ++ Sbjct: 428 QCLVVGAKDAGKTVFMQSLAGRGMADVAQIGRRH-SPFVINRVRVKEESKYLLLREVDVL 486

Query: 477 SESEFLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQ 536

S + L E DVV +YD+SNP SF +CA +++++F ++ PC+++A K + EV Q Sbjct: 487 SPQDALGSGETSADVVAFLYDISNPDSFAFCATVYQKYFYRTKTPCVMIATKVEREEVDQ 546

Query: 537 EYΞISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYP 580

+ + P +FCR+ ++P P F+ S IF +L MA+YP Sbjct: 547 RWEVPPEEFCRQFELPKPIKFSTGNIGQSSSPIFEQLAMMAVYP 590

Pedant information for DKFZphutel_22d2, frame 1

Report for DKFZphutel_22d2.1

[LENGTH] 580

[MW] 66541.61

[pi] 5.56

[HOMOL] TREMBL:CEUK08F11_3 gene: "K08F11.5"; Caenorhabditis elegans cosmid K08F11. le-

149

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YAL048c] 5e-81

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YKR055w]

3e-ll

[FUNCAT] 03.99 other cell growth, cell division and dna synthesis activities [S. cerevisiae, YNL098c] 8e-09

[FUNCAT] 10.04.07 g-proteins [S. cerevisiae, YNL098c] 8e-09

[FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YNL098c] 8e-09

[FUNCAT] 11.01 stress response [S. cerevisiae, YNL098c] 8e-09

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YNL098c] 8e-09

[FUNCAT] 01.03.13 regulation of nucleotide metabolism [S. cerevisiae, YNL098c]

8e-09

[ FUNCAT ] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YNL098c]

8e-09

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YORlOlw] 4e-08

[FUNCAT] 11.10 cell death [S. cerevisiae, YORlOlw] 4e-08

[FUNCAT] 10.02.07 g-proteins [S. cerevisiae, YPR165w] 7e-08

[FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YPR165w] 7e-08

[FUNCAT] 30.08 organization of golgi [S. cerevisiae, YPR165w] 7e-08

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YFL005w]

9e-08

[FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae,

YFL005w] 9e -08

[FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YFL005w] 9e-06

[FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YNL093w] le-07 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YNL093w] le-07

[FUNCAT] 08.19 cellular import [S. cerevisiae, YNL093w] le-07

[FUNCAT] 10.05.07 g-proteins [S. cerevisiae, YLR229C] 8e-07

[FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins

[S. cerevisiae, YLR229c] 8e-07 [FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YCR027c] 3e-06 [FUNCAT] 09.09 biogenesis of intracellular transport vesicles [S. cerevisiae, YGL210w] 9e-04 [BLOCKS] BL00410A Dynamin family proteins [SCOP] dlplk 3.25.1.3.1 cH-p21 Ras protein [human (Homo sapiens) 2e-42 [SCOP] dlguaa_ 3.25.1.3.10 RaplA [Human (Homo sapiens) 5e-59 [PIRKW] transmembrane protein le-79 [PIRKW] membrane trafficking 2e-06 [PIRKW] acetylated am o end 3e-09 [PIRKW] prenylated cysteine 3e-09 [PIRKW] signal transduction le-07 [PIRKW] transforming protein 3e-09 [PIRKW] immediate-early protein 8e-06 [PIRKW] alternative splicing 4e-08 [PIRKW] P-loop le-10 [PIRKW] lipoprotein 7e-10 [PIRKW] proto-oncogene 3e-09 [PIRKW] methylated carboxyl end 3e-09 [PIRKW] membrane protein 3e-09 [PIRKW] GTP binding le-10 [PIRKW] thiolester bond 7e-10 [SUPFAM] ras transforming protein le-10 [PROSITE] ATP_GTP_A 2 [PROSITE] MYRISTYL 3 [PROSITE] EF_HAND 1 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 14 [PROSITE] TYR_PHOSPHO_SITE 4 [PROSITE] PKC_PHOSPHO_SITE 5 [PROSITE] ASN_GLYCOSYLATION 3 [PFAM] Ras family (contains ATP/GTP binding P-loop) [KW] Irregular [KW] 3D

SEQ MKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSE ljai- ...EEEEEEEETTTTCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEETTEEEEEEEEECCC

SEQ AEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKS ljai- CGGGHHHHHHHHHHTTEEEEEEETTTHHHHHHH-HHHHHHHHHHHCTTT-TCEEEEEETT

SEQ DLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKE ljai- TTTTTTTTHHHHHHHHHHHCCCE-EECTTTTTTTHHHHHH

SEQ MKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHIΞDG

SEQ VADSGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTE

SEQ LNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFKVFPYIPWGPDVNNTVCTNERGWITYQ ljai-

SEQ GFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTRDKKIDLQKKQTQRNVFR

SEQ CNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDISESE

SEQ FLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSI

SEQ SPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYP

Prosite for DKFZphutel 22d2.1

PS00001 118->122 ASN_GLYCOSYLATION PDOC00001 PS00001 154->158 ASN_GLYCOSYLATION PDOC00001 PS00001 346->350 AΞN_GLYCOSYLATION PDOC00001 PS00004 411->415 CAMP_PHOSPHO_SITE PDOC00004 PS00005 94->97 PKC_PHOSPHO_SITE PDOC00005 PS00005 105->108 PKC PHOSPHO SITE PDOC00005 PS00005 148->151 PKC PHOSPHO_ SITE PDOC00005

PS00005 247->250 PKC PHOSPHO^ ^"SITE PDOC00005

PS00005 414->417 PKC PHOSPHO^{" "}SITE PDOC00005

PS00006 59->63 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 105->109 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 126->130 CK2 PHOSPHO^{"" "}SITE PDOC00006

PS00006 139->143 CK2 PHOSPHO_ ^"SITE PDOC00006

PS00006 143->147 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 196->200 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 203->207 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 311->315 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 325->329 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 370->374 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 390->394 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 477->481 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 483->487 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 541->545 CK2 PHOSPHO^{~ "}SITE PDOC00006

PS00007 153->161 TYR PHOSPHO_ ^"SITE PDOC00007

PS00007 376->384 TYR PHOSPHO ^"SITE PDOC00007

PS00007 153->162 TYR PHOSPHO ^"SITE PDOC00007

PS00007 448->457 TYR PHOSPHO^{" "}SITE PDOC00007

PS00008 240->246 MYRISTYL PDOC00008

PS00008 425->431 MYRISTYL PDOC00008

PS00008 433->439 MYRISTYL PDOC00008

PS00017 11->19 ATP GTP A PDOC00017

PS00017 425->433 ATP GTP A PDOC00017

PΞ00018 197->210 EF HAND PDOC00018

Pfam for DKFZphutel 22d2.1

HMM_NAME Ras family (contains ATP/GTP binding P-loop)

HMM *KLVLIGDSGVGKSCLLIRFTQNeFnEeYIPTIGvDFYtKTIEIDGKtIK ++L+G+ VGK++L ++ EF+EE +P ++ T ++ +++

Query 6 RILLVGEPRVGKTSLIMSLVSEEFPEE-VPPR-AEEITIPADVTPERVP 52

HMM LQIWDTAGQERYRsMRPMYYRGAMGFMLVYDITNRqSFENIr.NWweEIr I D E+ + + + +A+++ +VY+++N+ S ++++ +W++ 1+

Query 53 THIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLIN 102

HMM RHCDrDENVPIMLVGNKCDLEDQRQVStEEGQeFAREWGAIPFMETSAKT + D+D+ P +LVGNK+DL + ++T + +E+SAK+

Query 103 ERTDKDSRLPLILVGNKSDLVEYΞSMETILPIMNQYTEI-ETCVECSAKN 151

HMM NiNVEEAFMEIvRellqrMqeqNqteNimdQpsrnrkrCCCIM* N+ E F+ + +++L + +++ +++++ + C+

Query 152 LKNISELFYYAQKAVLHPT GPLYCPEEKEMK-PACI— 186

DKFZphutel_22el2

group: signal transduction

DKFZphutel_22el2 encodes a novel 92 amino acid protein, with similarity to yeast, C. elegans, Drosophila and mammalian proteins.

The Drosophila cm and mammalian cornicon proteins are part of a signal transduction pathway involving hte EGF-receptor.

The new protein can find application in modulating the cornichon modulated signal transduction way and also the EGF receptor signaling processes. strong similarity to S. cerevisiae YGL054c and cornichon complete cDNA, complete eds, EST hits cornicon is requiered for signal transduction in the EGF-receptor signal processing

Sequenced by BMFZ

Locus: unknown

Insert length: 519 bp

Poly A stretch at pos. 499, no polyadenylation signal found

1 GTCGGGGCAT CCGAGCGGGT TTGACGGAAG GAGCGGCGGC GACGGAGGAG 51 GAGGATGGAG GCGGTGGTGT TCGTCTTCTC TCTCCTCGAT TGTTGCGCGC 101 TCATCTTCCT CTCGGTCTAC TTCATAATTA CATTGTCTGA TTTAGAATGT 151 GATTACATTA ATGCTAGATC ATGTTGCTCA AAATTAAACA AGTGGGTAAT 201 TCCAGAATTG ATTGGCCATA CCATTGTCAC TGTATTACTG CTCATGTCAT 251 TGCACTGGTT CATCTTCCTT CTCAACTTAC CTGTTGCCAC TTGGAATATA 301 TATCGTATGA TCTTAGCTTT GATAAATGAC TGAAGCTGGA GAAGCCGTGG 351 TTGAAGTCAG CCTACACTAC AGTGCACAGT TGAGGAGCCA GAGACTTCTT 401 AAATCATCCT TAGAACCGTG ACCATAGCAG TATATATTTT CCTCTTGGAA 451 CAAAAAACTA TTTTTGCTGT ATTTTTACCA TATAAAGTAT TTAAAAAACA 501 TGAAAAAAAA AAAAAAAAA

BLAST Results

No BLAST result

Medline entries

95300228: cornichon and the EGF receptor signaling process are necessary for both anterior-posterior and dorsal-ventral pattern formation in Drosophila.

Peptide information for frame 1

ORF from 55 bp to 330 bp; peptide length: 92 Category: strong similarity to known protein

1 MEAVVFVFSL LDCCALIFLS VYFIITLSDL ECDYINARSC CSKLNKWVIP 51 ELIGHTIVTV LLLMSLHWFI FLLNLPVATW NIYRMILALI ND

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_22el2, frame 1

PIR:S64058 probable membrane protein YGL054c - yeast (Saccharomyces cerevisiae), N = 2, Score = 185, p = 5.7e-17

TREMBL :SPAC2C4_5 gene: "SPAC2C4.05"; product: "cornichon homolog"; S. pombe chromosome I cosmid c2C4. , N = 1, Score = 163, P = 3.7e-12

PIR:S46084 probable membrane protein YBR210w - yeast (Saccharomyces cerevisiae), N = 1, Score = 162, P = 4.8e-12

TREMBL:AF104398_1 product: "cornichon"; Homo sapiens cornichon mRNA, complete eds., N = 1, Score = 141, P = 8e-10

SWISSPROT :CNI_DROVI CORNICHON PROTEIN., N = 1, Score = 139, P = 1.3e-09

>PIR:S64058 probable membrane protein YGL054c - yeast (Saccharomyces cerevisiae)

Length = 138

HSPs:

Score = 185 (27.8 bits), Expect = 5.7e-17, Sum P(2) = 5.7e-17 Identities = 35/85 (41%), Positives = 56/85 (65%)

Query: 1 MEAVVFVFSLLDCCALIFLSVYFIITLSDLECDYINARSCCSKLNKWVIPELIGHTIVTV 60

M A +F+ +++ C +F V+F I +DLE DYIN CSK+NK + PE H +++ Sbjct: 1 MGAWLFILAVVVNCINLFGQVHFTILYADLEADYINPIELCSKVNKLITPEAALHGALSL 60

Query: 61 LLLMSLHWFIFLLNLPVATWNIYRM 85

L L++ +WF+FLLNLPV +N+ ++ Sbjct: 61 LFLLNGYWFVFLLNLPVLAYNLNKI 85

Score = 37 (5.6 bits), Expect = 5.7e-17, Sum P(2) = 5.7e-17 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 82 IYRMILALI 90

+YRMI+ALI Sbjct: 123 LYRMIMALI 131

Pedant information for DKFZphutel_22el2, frame 1

Report for DKFZphutel_22el2.1

[LENGTH] 92

[MW] 10614.98

[pi] 5.04

[HOMOL] PIR:S64058 probable membrane protein YGL054c - yeast (Saccharomyces cerevisiae) 5e-14

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YGL054c] 2e-15

[PIRKW] transmembrane protein 2e-ll

[PROSITE] CK2_PHOSPHO_SITE 3

[KW] SIGNAL_PEPTIDE 33

[KW] TRANSMEMBRANE 2

SEQ MEAVVFVFSLLDCCALIFLSVYFIITLSDLECDYINARSCCSKLNKWVIPELIGHTIVTV PRD ccchhhhhhhhhhhhhhhhhhhheeeccccccccccccccccccceeehhhhhhhhhhhh MEM MMMMMMMMMM

SEQ LLLMSLHWFIFLLNLPVATWNIYRMILALIND PRD hhhhhhhheeecccccchhhhhhhhhhhhccc MEM MMMMMMMMMMMMMMMMMMM .. MMMMMMM ....

Prosite for DKFZphutel 22el2.1

PS00006 9->13 CK2 PHOSPHO SITE PDOC00006

PS00006 26->30 CK2 PHOSPHO SITE PDOC00006

PS00006 28->32 CK2 PHOSPHO SITE PDOC00006

(No Pfam data available for DKFZphutel_22el2.1) DKFZphutel_22n2

group: uterus derived

DKFZphutel_22n2 encodes a novel 304 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of uterus-specific genes . unknown complete cDNA, complete eds, EST hits

Sequenced by BMFZ

Locus: /map="553.3 cR from top of Chrll linkage group"

Insert length: 1556 bp

Poly A stretch at pos. 1534, no polyadenylation signal found

1 ACAACAGGCT GGTTGCTTGG CGTGGAATCC TAAAGTGGCC TGGCTTTGAG

51 ACTGGAGTGA GACCCCAGCC CTAGGCTGGG GTTCTTTCCA TTATAGAGGA

101 GACGGATTCA GAAGGGCTAC AGACCAAGGT TGTTGAAAAC CAGACATATG

151 ATGAGCGTCT AGAGATTAAC GACTCCGAAG AGGTTGCAAG TATTTATACT

201 CCAACCCCAA GACACCAAGG ACTTCCTCGT TCTGCCCATC TTCCTAACAA

251 GGCTATGGCT GATAACAGCA GTGATGAGTG TGAAGAGGAA AATAACAAGG

301 AGAAGAAGAA GACCTCACAG TTGACACCTC AACGGGGCTT TAGTGAAAAT

351 GAGGATGACG ATGATGATGA TGATGATTCA TCTGAAACTG ATTCTGATTC

401 TGATGATGAT GATGAAGAGC ATGGAGCCCC TCTGGAAGGG GCCTATGACC

451 CTGCAGACTA TGAGCATTTG CCAGTTTCTG CTGAAATTAA GGAACTCTTC

501 CAGTACATCA GTAGGTACAC ACCTCAGTTG ATTGACCTGG ACCACAAACT

551 GAAGCCTTTC ATTCCTGATT TTATCCCAGC TGTCGGGGAT ATTGATGCAT

601 TCTTAAAGGT CCCACGTCCT GATGGAAAGC CTGACAACCT TGGCCTATTG

651 GTATTGGATG AACCTTCTAC AAAGCAGTCA GACCCTACGG TGCTCTCACT

701 CTGGTTAACA GAGAATTCTA AGCAGCACAA CATCACACAA CATATGAAAG

751 TAAAAAGCCT AGAAGATGCA GAAAAGAATC CCAAAGCCAT TGACACGTGG

801 ATTGAGAGCA TCTCTGAATT ACACCGTTCT AAGCCCCCTG CGACTGTGCA

851 CTACACCAGG CCCATGCCCG ACATTGACAC GCTGATGCAG GAATGGTCCC

901 CGGAGTTTGA AGAGCTTTTG GGCAAGGTAA GCCTGCCCAC GGCAGAGATT

951 GATTGCAGCC TGGCAGAGTA CATTGACATG ATCTGTGCCA TTCTAGACAT

1001 CCCTGTCTAC AAGAGTCGGA TCCAGTCCCT CCATCTGCTC TTTTCCCTCT

1051 ACTCAGAATT CAAGAACTCA CAGCATTTTA AAGCTCTCGC TGAAGGCAAG

1101 AAAGCATTCA CTCCTTCATC CAATTCCACC TCCCAAGCTG GAGACATGGA

1151 GACATTAACC TTCAGCTGAG ACACTTCCCA AGCTGCTGTT TCAAGGCTGA

1201 GCTGGCCCCT CTGCCCCAGC TGAGATGGAC AGATCGTTGT CAGCTACTTG

1251 ATGTCCTTGC CCATGCCACA GCTTGGCTCA GGGGCAGTGC ATGTCCTGCT

1301 GCCCTCTCTG CCAGAGGGCA CAGAACATGT TTGTTTAATG AACCTGCCTG

1351 CCTCAGATTG CTGTCCCCGG GGAGTTAATG CATCTACACC ACTGTGGGGA

1401 TTTGAGTTAT AAGAATTGGA ATTTCTGAGA TCCCATGGAG GTTAGATTGG

1451 GAGGAAAGCT TAAAAGATGT CCTTTTTGTG AGAGGGATGG AATTGTTTTC

1501 TTTCATTCGT AAAGTTAGTG AGTAAAGATT TTATAAATCA AAAAAAAAAA

1551 AAAAAA

BLAST Results

Entry HS188252 from database EMBL: human STS WI-12265. Score = 2554, P = 4.1e-109, identities 556/587

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 255 bp to 1166 bp; peptide length: 304 Category: putative protein 1 MADNSSDECE EENNKEKKKT SQLTPQRGFS ENEDDDDDDD DSSETDSDSD

51 DDDEEHGAPL EGAYDPADYE HLPVSAEIKE LFQYISRYTP QLIDLDHKLK

101 PFIPDFIPAV GDIDAFLKVP RPDGKPDNLG LLVLDEPSTK QSDPTVLSLW

151 LTENSKQHNI TQHMKVKSLE DAEKNPKAID TWIESISELH RSKPPATVHY

201 TRPMPDIDTL MQEWSPEFEE LLGKVSLPTA EIDCΞLAEYI DMICAILDIP

251 VYKSRIQSLH LLFSLYSEFK NSQHFKALAE GKKAFTPSSN STSQAGDMET

301 LTFS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_22n2, frame 3

PIR:S38149 SIS2 protein - yeast (Saccharomyces cerevisiae), N = 1, Score = 132, P = le-05

>PIR:S38149 SIS2 protein - yeast (Saccharomyces cerevisiae) Length = 562

HSPs:

Score = 132 (19.8 bits), Expect = 1.0e-05, P = 1.0e-05 Identities = 24/63 (38%), Positives = 35/63 (55%)

Query: 3 DNSSDECEEENNKEKKKTSQLTPQRGFSENEDDDDDDDDSSETDΞDSDDDDEEHGAPLEG 62

+ DE EEE++ E++ T +++DDDDDDDD + D D DDD++E A G

Sbjct: 497 EEDDDEDEEEDDDEEEDTEDKNENNNDDDDDDDDDDDDDDDDDDDDDDDDEDEDEAETPG 556

Query: 63 AYD 65

D Sbjct: 557 IID 559

Score = 122 (18.3 bits). Expect = 1.4e-04, P = 1.4e-04 Identities = 20/52 (38%), Positives = 33/52 (63%)

Query: 4 NSSDECEEENNKEKKKTSQLTPQRGFSENEDDDDDDDDSSETDSDSDDDDEE 55

N+ +E ++E+ +E + T + + N+DDDDDDDD + D D DDDD++ Sbjct: 494 NNEEEDDDEDEEEDDDEEEDTEDKNENNNDDDDDDDDDDDDDDDDDDDDDDD 545

Pedant information for DKFZphutel_22n2, frame 3

Report for DKFZphutel_22n2.3

[LENGTH] 304

[MW] 34285.85

[pi] 4.37

[PROSITE] AMIDATION 1

[PROSITE] CAMP_PHOSPHO_SITE 2

[PROSITE] CK2_PHOSPHO_SITE 10

[PROSITE] PKC_PHOSPHO_SITE 1

[PROSITE] ASN_GLYCOSYLATION 3

[KW] All_Alpha

[KW] LOW_COMPLEXITY 11.84 %

SEQ MADNSSDECEEENNKEKKKTSQLTPQRGFSENEDDDDDDDDSSETDSDSDDDDEEHGAPL

SEG xxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxx

PRD ccccccchhhhhhchhhhhhcccccccccccccccccccccccccccccccccccccccc

SEQ EGAYDPADYEHLPVΞAEIKELFQYISRYTPQLIDLDHKLKPFIPDFIPAVGDIDAFLKVP SEG

PRD ccccccccccccchhhhhhhhhhhhhhhccccccccccccccccccccccccccceeecc

SEQ RPDGKPDNLGLLVLDEPSTKQSDPTVLSLWLTENSKQHNITQHMKVKSLEDAEKNPKAID SEG

PRD ccccccccceeeeecccccccccccchhhhhhccccccccccccchhhhhhhhcccccch

SEQ TWIESISELHRSKPPATVHYTRPMPDIDTLMQEWSPEFEELLGKVSLPTAEIDCSLAEYI SEG

PRD hhhhhhhhhhcccccceeeeecccccchhhhhhcccchhhhhccccccccccchhhhhhh

SEQ DMICAILDIPVYKSRIQΞLHLLFSLYSEFKNSQHFKALAEGKKAFTPSSNSTSQAGDMET SEG PRD hhhhhhhcccchhhhhhhhhhhhhhhhhhhcchhhhhhhhcccccccccccccccccccc

SEQ LTFS

SEG ....

PRD cccc

Prosite for DKFZphutel_22n2.3

PS00001 4->8 ASN_GLYCOSYLATION PDOC00001

PS00001 159->163 ASN_GLYCOSYLATION PDOC00001

PS00001 290->294 ASN_GLYCOSYLATION PDOC00001

PS00004 17->21 CAMP_PHOSPHO_SITE PDOC00004

PS00004 18->22 CAMP_PHOSPHO_SITE PDOC00004

PS00005 138->141 PKC_PHOSPHO_SITE PDOC00005

PS00006 5->9 CK2_PHOSPHO_SITE PDOC00006

PS00006 30->34 CK2_PHOSPHO_SITE PDOC00006

PS00006 43->47 CK2_PHOSPHO_SITE PDOC00006

PS00006 45->49 CK2_PHOSPHO_SITE PDOC00006

PS00006 47->51 CK2_PHOSPHO_SITE PDOC00006

PS00006 49->53 CK2_PHOSPHO_SITE PDOC00006

PS00006 168->172 CK2_PHOSPHO_SITE PDOC00006

PS00006 181->185 CK2_PHOSPHO_SITE PDOC00006

PS00006 185->189 CK2_PHOSPHO_SITE PDOC00006

PS00006 235->239 CK2_PHOSPHO_SITE PDOC00006

PS00009 280->284 AMIDATION PDOC00009

(No Pfam data available for DKFZphutel_22n2.3)

DKFZphutel_22o2

group: uterus derived

DKFZphutel_22o2 encodes a novel 537 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of uterus-specific genes . similarity to S. pombe SPBC3E7.03c complete cDNA, complete eds, EST hits

Sequenced by BMFZ

Locus: map="llpl5.5"

Insert length: 2714 bp

Poly A stretch at pos. 2695, polyadenylation signal at pos. 2677

1 GCAGGGCACG GTGGGGGCTG AGATCGTTTC CTGTTGGAAC TTCTGGCCCA 51 AGAAGCGCGG GTCACAAGGA GAGGGGTCAG TTCGGTTCAG AGCGACTCAG

101 CCCCTCGACT CGGGTCTTAA AACCTCCGAG CCGCCAGTTC TGCCTCAGGC

151 CGCGCCCCCT TAAAGCGCCA CCAGACGCTG CGCCCCGTTA AAGCGCCACC

201 AGACGCCGCG CCCCGTCCCG GCCTCCCCCG CGCGCTGGCG CGGGGCTTTC

251 TGGGCCAGGG CGGGGCCGGC GAACTGCGGC CCGGAACGGC TGAGGAAGGG

301 CCCGTCCCGC CTTCCCCGGC GCGCCATGGA GCCCCGGGCG GTTGCAGAAG

351 CCGTGGAGAC GGGTGAGGAG GATGTGATTA TGGAAGCTCT GCGGTCATAC

401 AACCAGGAGC ACTCCCAGAG CTTCACGTTT GATGATGCCC AACAGGAGGA

451 CCGGAAGAGA CTGGCGGAGC TGCTGGTCTC CGTCCTGGAA CAGGGCTTGC

501 CACCCTCCCA CCGTGTCATC TGGCTGCAGA GTGTCCGAAT CCTGTCCCGG

551 GACCGCAACT GCCTGGACCC GTTCACCAGC CGCCAGAGCC TGCAGGCACT

601 AGCCTGCTAT GCTGACATCT CTGTCTCTGA GGGGTCCGTC CCAGAGTCCG

651 CAGACATGGA TGTTGTACTG GAGTCCCTCA AGTGCCTGTG CAACCTCGTG

701 CTCAGCAGCC CTGTGGCACA GATGCTGGCA GCAGAGGCCC GCCTAGTGGT

751 GAAGCTCACA GAGCGTGTGG GGCTGTACCG TGAGAGGAGC TTCCCCCACG

801 ATGTCCAGTT CTTTGACTTG CGGCTCCTCT TCCTGCTAAC GGCACTCCGC

851 ACCGATGTGC GCCAGCAGCT GTTTCAGGAG CTGAAAGGAG TGCGCCTGCT

901 AACTGACACA CTGGAGCTGA CGCTGGGGGT GACTCCTGAA GGGAACCCCC

951 CACCCACGCT CCTTCCTTCC CAAGAGACTG AGCGGGCCAT GGAGATCCTC 1001 AAAGTGCTCT TCAACATCAC CCTGGACTCC ATCAAGGGGG AGGTGGACGA 1051 GGAAGACGCT GCCCTTTACC GACACCTGGG GACCCTTCTC CGGCACTGTG 1101 TGATGATCGC TACTGCTGGA GACCGCACAG AGGAGTTCCA CGGCCACGCA 1151 GTGAACCTCC TGGGGAACTT GCCCCTCAAG TGTCTGGATG TTCTCCTCAC 1201 CCTGGAGCCA CATGGAGACT CCACGGAGTT CATGGGAGTG AATATGGATG 1251 TGATTCGTGC CCTCCTCATC TTCCTAGAGA AGCGTTTGCA CAAGACACAC 1301 AGGCTGAAGG AGAGTGTAGC TCCCGTGCTG AGCGTGCTGA CTGAATGTGC 1351 CCGGATGCAC CGCCCAGCCA GGAAGTTCCT GAAGGCCCAG GGATGGCCAC 1401 CTCCCCAGGT GCTGCCCCCT CTGCGGGATG TGAGGACACG GCCTGAGGTT 1451 GGGGAGATGC TGCGGAACAA GCTTGTCCGC CTCATGACAC ACCTGGACAC 1501 AGATGTGAAG AGGGTGGCTG CCGAGTTCTT GTTTGTCCTG TGCTCTGAGA 1551 GTGTGCCCCG ATTCATCAAG TACACAGGCT ATGGGAATGC TGCTGGCCTT 1601 CTGGCTGCCA GGGGCCTCAT GGCAGGAGGC CGGCCCGAGG GCCAGTACTC 1651 AGAGGATGAG GACACAGACA CAGATGAGTA CAAGGAAGCC AAAGCCAGCA 1701 TAAACCCTGT GACCGGGAGG GTGGAGGAGA AGCCGCCTAA CCCTATGGAG 1751 GGCATGACAG AGGAGCAGAA GGAGCACGAG GCCATGAAGC TGGTGACCAT 1801 GTTTGACAAG CTCTCCAGGA ACAGAGTCAT CCAGCCAATG GGGATGAGTC 1851 CCCGGGGTCA TCTTACGTCC CTGCAGGATG CCATGTGCGA GACTATGGAG 1901 CAGCAGCTCT CCTCGGACCC TGACTCGGAC CCTGACTGAG GATGGCAGCT 1951 CTTCTGCTCC CCCATCAGGA CTGGTGCTGC TTCCAGAGAC TTCCTTGGGG 2001 TTGCAACCTG GGGAAGCCAC ATCCCACTGG ATCCACACCC GCCCCCACTT 2051 CTCCATCTTA GAAACCCCTT CTCTTGACTC CCGTTCTGTT CATGATTTGC 2101 CTCTGGTCCA GTTTCTCATC TCTGGACTGC AACGGTCTTC TTGTGCTAGA 2151 ACTCAGGCTC AGCCTCGAAT TCCACAGACG AAGTACTTTC TTTTGTCTGC 2201 GCCAAGAGGA ATGTGTTCAG AAGCTGCTGC CTGAGGGCAG GGCCTACCTG 2251 GGCACACAGA AGAGCATATG GGAGGGCAGG GGTTTGGGTG TGGGTGCACA 2301 CAAAGCAAGC ACCATCTGGG ATTGGCACAC TGGCAGAGCC AGTGTGTTGG 2351 GGTATGTGCT GCACTTCCCA GGGAGAAAAC CTGTCAGAAC TTTCCATACG 2401 AGTATATCAG AACACACCCT TCCAAGGTAT GTATGCTCTG TTGTTCCTGT 2451 CCTGTCTTCA CTGAGCGCAG GGCTGGAGGC CTCTTAGACA TTCTCCTTGG 2501 TCCTCGTTCA GCTGCCCACT GTAGTATCCA CAGTGCCCGA GTTCTCGCTG 2551 GTTTTGGCAA TTAAACCTCC TTCCTACTGG TTTAGACTAC ACTTACAACA 2601 AGGAAAATGC CCCTCGTGTG ACCATAGATT GAGATTTATA CCACATACCA 2651 CACATAGCCA CAGAAACATC ATCTTGAAAT AAAGAAGAGT TTTGGACAAA 2701 AAAAAAAAAA AAAA BLAST Results

Entry AF015416 from database EMBL:

Homo sapiens chromosome 11 from llpl5.5 region, complete sequence.

Score = 3356, P = 2.0e-144, identities = 672/673

Entry HS263253 from database EMBL: human STS SHGC-15914. Score = 1143, P = 9.0e-46, identities = 245/255

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 326 bp to 1936 bp; peptide length: 537 Category: similarity to unknown protein

1 MEPRAVAEAV ETGEEDVIME ALRSYNQEHS QSFTFDDAQQ EDRKRLAELL

51 VSVLEQGLPP SHRVIWLQSV RILSRDRNCL DPFTSRQSLQ ALACYADISV

101 SEGSVPESAD MDVVLESLKC LCNLVLSSPV AQMLAAEARL VVKLTERVGL

151 YRERSFPHDV QFFDLRLLFL LTALRTDVRQ QLFQELKGVR LLTDTLELTL

201 GVTPEGNPPP TLLPSQETER AMEILKVLFN ITLDSIKGEV DEEDAALYRH

251 LGTLLRHCVM IATAGDRTEE FHGHAVNLLG NLPLKCLDVL LTLEPHGDST

301 EFMGVNMDVI RALLIFLEKR LHKTHRLKES VAPVLSVLTE CARMHRPARK

351 FLKAQGWPPP QVLPPLRDVR TRPEVGEMLR NKLVRLMTHL DTDVKRVAAE

401 FLFVLCΞESV PRFIKYTGYG NAAGLLAARG LMAGGRPEGQ YSEDEDTDTD

451 EYKEAKASIN PVTGRVEEKP PNPMEGMTEE QKEHEAMKLV TMFDKLSRNR

501 VIQPMGMSPR GHLTSLQDAM CETMEQQLSS DPDSDPD

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_22o2, frame 2

TREMBL: SPBC3E7_3 gene: "SPBC3E7.03c"; product: "hypothetical protein"; S. pombe chromosome II cosmid c3E7. , N = 1, Score = 112, P = 0.0023

>TREMBL:SPBC3E7_3 gene: "SPBC3E7.03c"; product: "hypothetical protein"; S. pombe chromosome II cosmid c3E7. Length = 362

HSPs:

Score = 112 (16.8 bits), Expect = 2.3e-03, P = 2.3e-03 Identities = 71/289 (24%), Positives = 124/289 (42%)

Query: 215 SQETERAM-EILKVLFNITLDSIKGEVDEEDAALYRHLGTLLRHCVMIATAGDRTEEFHG 273

SQ+ E + EIL++LF 1+ S E DE+ L L+ + + Sbjct: 12 ΞQDNEMVLTEILRLLFPISKRSYLKEEDEQKILL LVIEIWASSLNNNPNSPLRW 65

Query: 274 HAVN-LLG-NLPLKCLDVLLTLEPHGDSTEFMGVNMDVIRALLIFLEKRLHKTH RL 327

HA N LL NL L LD + + T + +1 + +LEK L+ + Sbjct: 66 HATNALLSFNLQLLSLDQAIYVSEIACQT LQSILISREVEYLEKGLNLCFDIAAKY 121

Query: 328 KESVAPVLSVLTECARMHRPARKFLKAQGWPPPQVLPPLRDVRTRP-EVGEMLRNKLVRL 386

+ ++ P+L++L + +L P D R + + G+ R L+RL

Sbjct: 122 QNTLPPILAILLSLLSFFNIKQNL SMLLFPTNDDRKQSLQKGKSFRCLLLRL 173

Query: 387 MT-HLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYΞ 442

+T + + A L LC + + G G A G+ M P + + Sbjct: 174 LTIPIVEPIGTYYASLLNELCDGDSQQIARIFGAGYAMGISQHSETMPFPSPLSKAASPV 233

Query: 443 -EDEDTDTDEYKEAKASINPVTGRV—EEKPPNPMEGMTEEQKEHEAMKLVTMFDKLSRN 499

+ + +E +I+P+TG + +E +++E+KE EA +L +F +L +N

Sbjct: 234 FQKNSRGQENTEENNLAIDPITGSMCTNRNKSQRLE-LSQEEKEREAERLFYLFQRLEKN 292 Query: 500 RVIQ 503

IQ Sbjct: 293 STIQ 296

Pedant information for DKFZphutel_22o2, frame 2

Report for DKFZphutel_22o2.2

[LENGTH] 537

[MW] 6037 2.53

[pi] 5.20

[BLOCKS] BL00 415L Synapsms proteins

[PROSITE] MYRI STYL 4

[PROSITE] CK2 PHOSPHO_SITE 13

[PROSITE] PKC PHOSPHO_SITE 10

[PROSITE] ASN GLYCOSYLATION 1

[KW] All Alpha

[KW] LOW^' COMPLEXITY 9.50 %

SEQ MEPRAVAEAVETGEEDVIMEALRSYNQEHSQSFTFDDAQQEDRKRLAELLVSVLEQGLPP SEG PRD ccchhhhhhhhhccchhhhhhhhhhccccccceeeccchhhhhhhhhhhhhhhhhccccc

SEQ SHRVIWLQSVRILSRDRNCLDPFTSRQSLQALACYADISVSEGSVPESADMDVVLESLKC SEG PRD cceeeeeccccccccccccccccchhhhhhhhhhhhceeeeccccccccchhhhhhhhhh

SEQ LCNLVLSSPVAQMLAAEARLVVKLTERVGLYRERSFPHDVQFFDLRLLFLLTALRTDVRQ SEG xxxxxxxxxxxxxxx ... PRD hhhhccccchhhhhhhhhhhhhhhhhccccccccccccccchhhhhhhhhhhhhhhhhhh

SEQ QLFQELKGVRLLTDTLELTLGVTPEGNPPPTLLPSQETERAMEILKVLFNITLDSIKGEV SEG PRD hhhhhhchhhhhhhhhhhhccccccccccccccchhhhhhhhhhhhhhhhhhccccchhh

SEQ DEEDAALYRHLGTLLRHCVMIATAGDRTEEFHGHAVNLLGNLPLKCLDVLLTLEPHGDST SEG PRD hhhhhhhhhhhhhhhhhhhhccccccccccccceeeeecccccccceeeeeeeccccccc

SEQ EFMGVNMDVIRALLIFLEKRLHKTHRLKESVAPVLSVLTECARMHRPARKFLKAQGWPPP

SEG PRD eeeehhhhhhhhhhhhhhhhhhhhhhccccceeeehhhhhhhhhchhhhhhhhccccccc

SEQ QVLPPLRDVRTRPEVGEMLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYG

SEG xxx

PRD cccccccccccchhhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhcccccceeeecccc

SEQ NAAGLLAARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEE

SEG xxxxxxxxxxxxxxx xxxxxxxxx

PRD chhhhhhhhhccccccccccccccccccchhhhhhhhhccccccceeecccccccchhhh

SEQ QKEHEAMKLVTMFDKLSRNRVIQPMGMSPRGHLTSLQDAMCETMEQQLSSDPDSDPD

SEG xxxxxxxxx

PRD hhhhhhhhhhhhhhhcccccccccccccccccchhhhhhhhhhhhhhhhcccccccc

Prosite for DKFZphutel_22o2.2

PS00001 230->234 ASN GLYCOSYLATION PDOC00001

PS00005 61->64 PKC _PHOSPHO_SITE PDOC00005

PS00005 69->72 PKC PHOSPHO_SITE PDOC00005

PΞ00005 84->87 PKC ^~PHOSPHO_SITE PDOC00005

PS00005 117-M20 PKC PHOSPHO_SITE PDOC00005

PS00005 145->148 PKC ^_PHOSPHO_SITE PDOC00005

PS00005 218->221 PKC ^"PHOSPHO_SITE PDOC00005

PS00005 235->238 PKC ^~PHOSPHO_SITE PDOC00005

PS00005 324->327 PKC PHOSPHO_SITE PDOC00005

PS00005 463->466 PKC ^"PHOSPHO_SITE PDOC00005

PS00005 508->511 PKC PHOSPHO_SITE PDOC00005

PS00006 12->16 CK2 ^"PHOSPHO_SITE PDOC00006

PS00006 34->38 CK2 PHOSPHO_SITE PDOC00006

PS00006 52->56 CK2 ^"PHOSPHO_SITE PDOC00006

PS00006 99->103 CK2 PHOSPHO_SITE PDOC00006

PS00006 104->108 CK2 ^"PHOSPHO_SITE PDOC00006

PS00006 263->267 CK2 ^"PHOSPHO_SITE PDOC00006

PS00006 371->375 CK2 ^"PHOSPHO SITE PDOC00006 PS00006 388->392 CK2_PHOSPHO_SITE PDOC00006 PS00006 442->446 CK2_PHOSPHO_SITE PDOC00006 PS00006 447->451 CK2_PHOSPHO_SITE PDOC00006 PS00006 491->495 CK2_PHOSPHO_SITE PDOC00006 PS00006 515->519 CK2_PHOSPHO_SITE PDOC00006 PS00006 530->534 CK2_PHOΞPHO_SITE PDOC00006 PS00008 57->63 MYRISTYL PDOC00008 PS00008 420->426 MYRISTYL PDOC00008 PS00008 424->430 MYRISTYL PDOC00008 PS00008 430->436 MYRISTYL PDOC00008

(No Pfam data available for DKFZphutel_22o2.2)

DKFZphutel_23el3

group: metabolism

DKFZphtes3_15j 18 encodes a novel 148 amino acid protein with similarity to 27K heat shock proteins .

The novel protein contains a serine protease of the subtilase family w th an aspartic acid- conta nmg active site. Subtilases are an extensive family of serine proteases whose catalytic activity is provided by a charge relay system similar to that of the trypsin family of serine proteases but which evolved by independent convergent evolution. The sequence around the residues involved in the catalytic triad (aspartic acid, serine and histidine) are completely different from that of the analogous residues in the trypsin serine proteases. Thus the novel protein is a new member of this family.

The new protein can find application in modulation of proteinase activity in cells and as a new enzyme for proteomics and biotechnologic production processes. heat shock protein HSP27 strong similarity to heat shock 27K proteins complete cDNA, complete eds, EST hits

Sequenced by EMBL

Locus: /map="578.9 cR from top of Chrl2 linkage group"

Insert length: 1854 bp

Poly A stretch at pos. 1831, polyadenylation signal at pos. 1810

1 GGTTTATTAA GCTCCTGGCT CCGCTCTAGA CCTCAGCGGT TCTGGCTGCC

51 AGCCTGGGCA GCCTGGGAAG CCTGGGAGGA CGGTGGCTTG CCGGTCTGTC

101 GTGAGGCAGT GCGGACGGGG ACCCTCTGGG ATTCTGCTGG ATCTGCCCCG

151 GGGGTTACCT TTGGGGGCTG GGACCCCAGT CGAGGGGACA CAACCGTCCC

201 TGGCAGTGGT TGGTTCTGCT TCTCCCTGCA GAAAAGCAGC ATTTTCGGAA

251 GCTGAAGAAT AAGCTAGCCC AGCCACACCA CCTTGTTGTG TGACCTTGGG

301 CAGGTGGTTC TGTCTCTCTG AGCCTCTGTT TCTCTCTGAG CTGAGCAGCC

351 ACCATGGCTG ACGGTCAGAT GCCCTTCTCC TGCCACTACC CAAGCCGCCT

401 GCGCCGAGAC CCCTTCCGGG ACTCTCCCCT CTCCTCTCGC CTGCTGGATG

451 ATGGCTTTGG CATGGACCCC TTCCCAGACG ACTTGACAGC CTCTTGGCCC

501 GACTGGGCTC TGCCTCGTCT CTCCTCCGCC TGGCCAGGCA CCCTAAGGTC

551 GGGCATGGTG CCCCGGGGCC CCACTGCCAC CGCCAGGTTT GGGGTGCCTG

601 CCGAGGGCAG GACCCCCCCA CCCTTCCCTG GGGAGCCCTG GAAAGTGTGT

651 GTGAATGTGC ACAGCTTCAA GCCAGAGGAG TTGATGGTGA AGACCAAAGA

701 TGGATACGTG GAGGTGTCTG GCAAACATGA AGAGAAACAG CAAGAAGGTG

751 GCATTGTTTC TAAGAACTTC ACAAAGAAAA TCCAGCTTCC TGCAGAGGTG

801 GATCCTGTGA CAGTATTTGC CTCACTTTCC CCAGAGGGTC TGCTGATCAT

851 CGAAGCTCCC CAGGTCCCTC CTTACTCAAC ATTTGGAGAG AGCAGTTTCA

901 ACAACGAGCT TCCCCAGGAC AGCCAGGAAG TCACCTGTAC CTGAGATGCC

951 AGTACTGGCC CATCCTTGTT TTGTCCCCAA CCCTAGGGCT TCTCTGATTC

1001 CAGGATACAT TACTTTAGCT GAACTCAGAT TTAGTGCAAG TAAAATGTTA

1051 GAGGGTGCGG GGGTGAGGAC TGACCACAGA TTCCCTGGAT AGTGTAGTGG

1101 TAGATTTCTC CACAGGATAG CGCAATTGGC AAATCATGCT TGGTTGTGTT

1151 AGGCCAAAAT ACTAGTTTTG CTTTCTTTAC CTTTTCTATC TTGATGAAAA

1201 TGTTGCACAT TCTATAGTTG CAAAACACAT AAAAGGGGAC TTAACATTTC

1251 ACGTTGTATC TTACTTGCAG TGAATGCAAG GGTTACTTTT CTCTGGGGAC

1301 CTCCCCCATC ACCCAGGTTC CTACTCTGGG CTCCCGATTC CCATGGCTCC

1351 CAAACCATGC CGCATGGTTT GGTTAATGAA ACCCAGTAGC TAACCCCACT

1401 GTGCTTCCAC ATGCCTGGCC TAAAATGGGT GATATACAGG TCTTATATCC

1451 CCATATGGAA TTTATCCATC AACCACATAA AAACAAACAG TGCCTTCTGC

1501 CCTCTGCCCA GATGTGTCCA GCACGTTCTC AAAGTTTCCA CATTAGCACT

1551 CCCTAAGGAC GCTGGGAGCC TGTCAGTTTA TGATCTGACC TAGGTCCCCC

1601 CTTTCTTCTG TCCCCTGTGT TTAAGTCGGG ATTTTTACAG AGGGAGCTGT

1651 CTCCAGACAG CTCCATCAGG AACCAAGCAA AGGCCAGATA GCCTGACAGA

1701 TAGGCTAGTG GTATTGTGTA TATGGGCGGG ACGTGTGTGT CATTATTATT

1751 TGAGTTATGC TGTTGTTTAG GGGTAAATAA CAGTAAATAA TTAATAATAA

1801 TAATAATAAT AATAAAGGAG CTGACGTTCT TAAAAAAGAA AAAAAAAAAA

1851 AAAA

BLAST Results

Entry HS286348 from database EMBL: human STS TIGR-A002J47. Score = 510, P = 1.2e-16, identities = 102/102 Medline entries

95394379:

Cloning and sequencing of a cDNA encoding the canine HSP27 protein.

94110260:

Physiological and pathological changes in levels of the two small stress proteins, HSP27 and alpha B crystallin, in rat hindlimb muscles

Peptide information for frame 3

ORF from 354 bp to 941 bp; peptide length: 196 Category: strong similarity to known protein Prosite motifs: SUBTILASE ASP (28-39)

1 MADGQMPFSC HYPSRLRRDP FRDSPLSSRL LDDGFGMDPF PDDLTASWPD 51 WALPRLSSAW PGTLRΞGMVP RGPTATARFG VPAEGRTPPP FPGEPWKVCV 101 NVHSFKPEEL MVKTKDGYVE VSGKHEEKQQ EGGIVSKNFT KKIQLPAEVD 151 PVTVFASLSP EGLLIIEAPQ VPPYSTFGES SFNNELPQDS QEVTCT

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_23el3, frame 3

PIR:JC4244 heat-shock 27K protein - dog, N = 1, Score = 304, P = 4.3e-27

PIR:JN0924 heat shock 27 protein - rat, N = 1, Score = 301, P = 8.9e-27

TREMBL :MM03561_1 product: "heat shock protein HSP27"; Mus musculus heat shock protein HSP27 internal deletion variant b mRNA, complete eds., N = 1, Score = 301, P = 8.9e-27

>PIR:JC4244 heat-shock 27K protein - dog Length = 209

HSPs:

Score = 304 (45.6 bits), Expect = 4.3e-27, P = 4.3e-27 Identities = 80/182 (43%), Positives = 102/182 (56%)

Query: 1 MADGQMPFSC-HYPSRLRRDPFRD-SPLSSRLLDDGFGMDPFPDDLTASWPDWALPRLSS 58

M + ++PFS PS DPFRD P SRL D FG+ P++ W W S Sbjct: 1 MTERRVPFSLLRSPSW DPFRDWYPAHSRLFDQAFGLPRLPEE WAQWFG HS 50

Query: 59 AWPGTLRSGMVP RGPTATARFGVPAEGR—TPPPFPG EPWKVCVNVHSF 105

WPG +R +P GP A A PA R + G + W+V ++V+ F Sbjct: 51 GWPGYVRP—IPPAVEGPAAAAAAAAPAYSRALSRQLSSGVSEIRQTADRWRVSLDVNHF 108

Query: 106 KPEELMVKTKDGYVEVSGKHEEKQQEGGIVSKNFTKKIQLPAEVDPVTVFASLSPEGLLI 165

PEEL VKTKDG VE++GKHEE+Q E G +S+ T K LP VDP V +SLSPEG L Sbjct: 109 APEELTVKTKDGVVEITGKHEERQDEHGYISRRLTPKYTLPPGVDPTLVSSSLSPEGTLT 168

Query: 166 IEAPQVPPYSTFGE 179

+EAP P + E Sbjct: 169 VEAPMPKPATQSAE 182

Pedant information for DKFZphutel_23el3, frame 3

Report for DKFZphutel_23el3.3

[LENGTH] 196

[MW] 21604.37 [pi] 5.00

[HOMOL] PIR:JC4244 heat-shock 27K protein - dog 3e-22

[BLOCKS] BL01031C

[PIRKW] blocked ammo end le-13

[PIRKW] acetylated ammo end 4e-13

[PIRKW] phosphoprotem 7e-21

[PIRKW] glycoprotein 2e-ll

[PIRKW] heat shock 7e-21

[PIRKW] molecular chaperone 4e-13

[PIRKW] alternative splicing le-19

[PIRKW] eye lens 6e-14

[PIRKW] stress-induced protein 7e-21

[SUPFAM] alpha-crystallin 7e-21

[PROSITE] SUBTILASE_ASP 1

[PROSITE] MYRISTYL 2

[PROSITE] CK2_PHOSPHO_SITE 2

[PROSITE] PKC_PHOSPHO_SITE 6

[PROSITE] ASN_GLYCOSYLATION 1

[PFAM] Heat shock hsp20 proteins

[KW] All_Beta

[KW] LOW COMPLEXITY 7.14 %

SEQ MADGQMPFΞCHYPSRLRRDPFRDSPLSSRLLDDGFGMDPFPDDLTASWPDWALPRLSSAW SEG xxxxxxxxxxxxxx

PRD ccccccccccccccccccccccccccchhhhhcccccccccccccccccccccccccccc

SEQ PGTLRSGMVPRGPTATARFGVPAEGRTPPPFPGEPWKVCVNVHSFKPEELMVKTKDGYVE SEG PRD cccccccccccccchhhhhhhhccccccchhhhhhheeeeeecccccceeeeecccceee

SEQ VSGKHEEKQQEGGIVSKNFTKKIQLPAEVDPVTVFAΞLSPEGLLIIEAPQVPPYSTFGES SEG PRD eccchhhhhcccceeeeccccccccccccccceeeecccccceeeeeccccccccccccc

SEQ SFNNELPQDSQEVTCT SEG PRD cccccccccceeeccc

Prosite for DKFZphutel_23el3.3

PS00001 138->142 ASN_GLYCOSYLATION PDOC00001

PS00005 27->30 PKC_PHOSPHO_SITE PDOC00005

PS00005 63->66 PKC_PHOSPHO_SITE PDOC00005

PS00005 76->79 PKC_PHOSPHO_SITE PDOC00005

PS00005 104->107 PKC_PHOSPHO_SITE PDOC00005

PS00005 122->125 PKC_PHOSPHO_SITE PDOC00005

PS00005 140->143 PKC_PHOSPHO_SITE PDOC00005

PS00006 47->51 CK2_PHOSPHO_SITE PDOC00006

PS00006 176->180 CK2_PHOSPHO_SITE PDOC00006

PS00008 62->68 MYRISTYL PDOC00008

PS00008 132->138 MYRISTYL PDOC00008

PS00136 28->39 SUBTILASE ASP PDOC00125

Pfam for DKFZphutel_23el3.3

HMM_NAME Heat shock hsp20 proteins

HMM *AMMrpPWDWRE DpDHFeVrMDMPGFKPEEIKVkVEDNNVLvIeG

A P++ R + ++V++++ FKPEE+ VK+ D+ +++++G

Query 77 ARFGVPAEGR-TPPPFPGEPWKVCVNVHSFKPEELMVKTKDG-YVEVSG 123

HMM EHEREEEREDDkWWWHERIYRHFMRRFrLPENVDpDqlkAsMSdNGVLTI +HE E++ + + ++ F +++LP +VDP + AS+S++G+L I

Query 124 KHE EKQQ EGGIVSKNFTKKIQLPAEVDPVTVFASLSPEGLLII 166

HMM TVPKpEP* ++P ++P

Query 167 EAPQVPP 173 DKFZphutel_23gll

group: uterus derived

DKFZphutel_23gll encodes a novel 256 amino acid protein with similarity to S. pombe SPAC31G5.12c and S. cerevisiae Maflp.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application m studying the expression profile of uterus-specific genes . similarity to SPAC31G5.12c and Maflp complete cDNA, complete eds, EST hits

Sequenced by EMBL

Locus : unknown

Insert length: 1674 bp

Poly A stretch at pos. 1664, polyadenylation signal at pos. 1644

1 GGGGGAGGCG GAGGTCGCTC GCTCGCTCGC TCGGCTCGCT GACTCGCCGG

51 AGCGCTCTGT GGCGGTCGGC GGCAGGTCGG TCGCGAGAGC GGGCTCTGTG

101 GAAGGGGGCG AGGCTATGTC GCGGTGGCAG CCCGGATGGG CCGGCAGGGC

151 CGGGAGTAAC GGGACGTCGC CGCGGAGCTT CTTCCCCCGG ATACAGTGCG

201 GCCCGAGCGG AGGCCGCGGC GCCGCCCTCC GATCTTGAAG AGCCCGCGCT

251 GCGCGGAGCC CGCCCCCGCC TGCGCACCGG CACCGACGCG GAGCGACCAG

301 CCCAGCCAGA CCCGGCCCGG CGCGGCCTGA TCTAACCCAG CCAGGCAGGC

351 AATACTAGCC CCTCTGGAGC ACGGAGCTCC TTCCCCAAAG ACATGAAGCT

401 ATTGGAGAAC TCGAGCTTTG AAGCCATCAA CTCACAGCTG ACTGTGGAGA

451 CCGGAGATGC CCACATCATT GGCAGGATTG AGAGCTACTC ATGTAAGATG

501 GCAGGAGACG ACAAACACAT GTTCAAGCAG TTCTGCCAGG AGGGCCAGCC

551 CCACGTGCTG GAGGCACTTT CTCCACCCCA GACTTCAGGA CTGAGCCCCA

601 GCAGACTCAG CAAAAGCCAA GGCGGTGAGG AGGAGGGCCC CCTCAGTGAC

651 AAGTGCAGCC GCAAGACCCT CTTCTACCTG ATTGCCACGC TCAATGAGTC

701 CTTCAGGCCT GACTATGACT TCAGCACAGC CCGCAGCCAT GAGTTCAGCC

751 GGGAGCCCAG CCTTAGCTGG GTGGTGAATG CAGTCAACTG CAGTCTGTTC

801 TCAGCTGTGC GGGAGGACTT CAAGGATCTG AAACCACAGC TGTGGAACGC

851 GGTGGACGAG GAGATCTGCC TGGCTGAATG TGACATCTAC AGCTATAACC

901 CAGACTTGGA CTCAGATCCC TTCGGGGAGG ATGGTAGCCT CTGGTCCTTC

951 AACTACTTCT TCTACAACAA GCGGCTCAAG CGAATCGTCT TCTTTAGCTG

1001 CCGTTCCATC AGTGGCTCCA CCTACACACC CTCAGAGGCA GGCAACGAGC

1051 TGGACATGGA GCTGGGGGAG GAGGAGGTGG AGGAAGAAAG CAGAAGCAGG

1101 GGCAGTGGGG CCGAGGAGAC CAGCACCATG GAGGAGGACA GGGTCCCAGT

1151 GATCTGTATT TGATGAGGAG GAGCCGAGGC CCCAGCTTCA TCCAGCTTCA

1201 ACCAATGCCT GGACCTGTCC ACCTGAGAGG CCCCTGGGGC CTCCCCAGCT

1251 GCTGGCCAGA CCCTGGCGCT GCCACAGTCC TGGCACTGCC CAAGGCCATA

1301 CCTGCCTAGC CCTTTGGCTC CATCCTGTGG ATGCCCACTC ACCCCTCAGA

1351 CTCCTGCTGC CCATGCTGTG GCCGGACTTG TCAGCAGGGG GCCTGGTGGG

1401 AGGAGCGACT GCCCTGCCCA AATGAACTGC CACAGCAGGG ACAGCTGGAC

1451 CGCAGAGTTT ATTTTTGTAT TTCTACTGGG CCTGCACACT CCAGCCCAAA

1501 GGGTCTGTGG CCGGAGGCCC CACGAGCAGG CCCCAGCAGT CACCGGCTCT

1551 GGTCTTGGGC CGGCCCCGGT GCCCACCTGT ACCCCCACCT CGCCCATTTG

1601 GCCGCGTGCA CTGAGTGTCA CTTTGCTGCA GCTCGTTTCT TTCCAATAAA

1651 AGTTTCTGTG ACTTAAAAAA AAAA

BLAST Results

No BLAST result

Medlme entries

No Medline entry

Peptide information for frame 3

ORF from 393 bp to 1160 bp; peptide length: 256 Category: similarity to known protein 1 MKLLENSSFE AINSQLTVET GDAHIIGRIE SYSCKMAGDD KHMFKQFCQE 51 GQPHVLEALS PPQTSGLSPS RLSKSQGGEE EGPLSDKCSR KTLFYLIATL 101 NESFRPDYDF STARSHEFSR EPSLSWVVNA VNCSLFSAVR EDFKDLKPQL 151 WNAVDEEICL AECDIYSYNP DLDSDPFGED GΞLWSFNYFF YNKRLKRIVF 201 FSCRSISGST YTPSEAGNEL DMELGEEEVE EESRΞRGSGA EETSTMEEDR 251 VPVICI

BLASTP hits

Entry SPAC31G5_12 from database TREMBL: gene: "SPAC31G5.12c"; product: "hypothetical protein"; S. pombe chromosome I cosmid c31G5.

Score = 272, P = 9.3e-24, identities = 51/127, positives = 80/127

Entry SPD656_1 from database TREMBL: product: "ORF N150"; Yeast DNA for bfr2+ proteιn/padl+ protem/sksl+ protein, ORF N313, ORF N150, complete eds, and for ORF N118, partial eds . Score = 263, P = 8.4e-23, identities = 50/127, positives = 79/127

Entry S50986 from database PIR:

MAF1 protein - yeast (Saccharomyces cerevisiae) >SWIΞSPROT :MAF1_YEAST

MAF1 PROTEIN. >TREMBL : SC19492_1 gene: "MAF1"; product: "Maflp";

Saccharomyces cerevisiae Maflp (MAF1) gene, complete eds.

>TREMBL:SC8119_11 gene: "MAFlp"; product: "Maflp"; S. cerevisiae chromosome IV cosmid 8119.

Score = 180, P = 2.3e-17, identities = 43/133, positives = 75/133

Entry AF098499_2 from database TREMBL: gene: "C43H8.2"; Caenorhabditis elegans cosmid C43H8.

Score = 263, P = 9.2e-23, identities = 78/252, positives = 118/252

Alert BLASTP hits for DKFZphutel_23gll, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphutel_23gll, frame 3

Report for DKFZphutel_23gll .3

[LENGTH] 256

[MW] 28869.95

[pi] 4.51

[HOMOL] TREMBL :SPAC31G5_12 gene: "SPAC31G5.12c"; product: "hypothetical protein";

S. pombe chromosome I cosmid C31G5. 4e-23

[FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YDR005c]

6e-13

[PROSITE] MYRISTYL 3

[PROSITE] CK2_PHOSPHO_SITE 5

[PROSITE] PKC_PHOSPHO_SITE 6

[PROSITE] ASN_GLYCOSYLATION 3

[KW] All_Alpha

[KW] LOW_COMPLEXITY 7.81 %

SEQ MKLLENSSFEAINSQLTVETGDAHIIGRIESYSCKMAGDDKHMFKQFCQEGQPHVLEALS SEG

PRD cccccchhhhhhhhhhhhccccceeeeecccchhhhhccchhhhhhhhhcccceeeeccc

SEQ PPQTSGLSPSRLSKSQGGEEEGPLSDKCSRKTLFYLIATLNEΞFRPDYDFSTARSHEFSR SEG

PRD cccccccccccccccccccccccccccchhhhhhhhhhhhcccccccccccccccccccc

SEQ EPSLSWVVNAVNCSLFSAVREDFKDLKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGED SEG

PRD ccccccchhhhhhhhhhhhhchhhhhhhhhhhhhhhhccccccceeeccccccccccccc

SEQ GSLWΞFNYFFYNKRLKRIVFFSCRSISGSTYTPSEAGNELDMELGEEEVEEESRSRGSGA

SEG xxxxxxxxxxxxxxxxxx

PRD ccceeeceeechhhhhhhhhhhccccccccccccccccchhhhhhhhhhhhhhccccccc

SEQ EETSTMEEDRVPVICI SEG XX

PRD cccccccccceeeccc Prosite for DKFZphutel_23gll .3

PS00001 6->10 ASN_GLYCOSYLATION PDOC00001 PS00001 101->105 ASN_GLYCOSYLATION PDOC00001 PS00001 132->136 ASN_GLYCOSYLATION PDOC00001 PS00005 33->36 PKC_PHOSPHO_SITE PDOC00005 PS00005 85->88 PKC_PHOSPHO_SITE PDOC00005 PS00005 89->92 PKC_PHOSPHO_SITE PDOC00005 PS00005 103->106 PKC_PHOSPHO_SITE PDOC00005 PS00005 112->115 PKC_PHOSPHO_SITE PDOC00005 PS00005 202->205 PKC_PHOSPHO_SITE PDOC00005 PS00006 7->ll CK2_PHOSPHO_SITE PDOC00006 PS00006 99->103 CK2_PHOSPHO_SITE PDOC00006 PS00006 212->216 CK2_PHOSPHO_SITE PDOC00006 PS00006 238->242 CK2_PHOSPHO_SITE PDOC00006 PS00006 244->248 CK2_PHOSPHO_SITE PDOC00006 PS00008 66->7₂ MYRISTYL PDOC00008 PS00008 181-M87 MYRISTYL PDOC00008 PS00008 239->245 MYRISTYL PDOC00008

(No Pfam data available for DKFZphutel_23gll .3)

DKFZphutel_24cl9

group: transmembrane protein

DKFZphutel_24cl9 encodes a novel 195 amino ac d protein without similarity to known proteins.

The novel protein contains 1 transmembrane region.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of uterus-specific genes and as a new marker for uterine cells. unknown membrane regions: 1

Summary DKFZphutel_24cl9 encodes a novel 195 amino acid protein, with no similarity to known proteins. unknown complete cDNA, complete eds, EST hits TRANSMEMBRANE 1

Sequenced by Qiagen

Locus: unknown

Insert length: 769 bp

Poly A stretch at pos. 746, polyadenylation signal at pos. 735

1 ACGAGTCAGC CAAAGATGGC TGCGCCCAGG TAATTTGAGC AAAGGCCACA

51 GTGAACTCCG GCGTGGCTGA GGAAGACCGG AGGAGGCACC CACAGGCTGC

101 TGGGAGGAGA GCATAAGGCT CAAAATGGAA AATCATAAAT CCAATAATAA

151 GGAAAACATA ACAATTGTTG ATATATCCAG AAAAATTAAC CAGCTTCCAG

201 AAGCAGAAAG GAATCTACTT GAAAATGGAT CGGTTTATGT TGGATTAAAT

251 GCTGCTCTTT GTGGCCTCAT AGCAAACAGT CTTTTTCGAC GCATCTTGAA

301 TGTGACAAAG GCTCGCATAG CTGCTGGCTT ACCAATGGCA GGGATACCTT

351 TTCTTACAAC AGACTTAACT TACAGATGTT TTGTAAGTTT TCCTTTGAAT

401 ACAGGTGATT TGGATTGTGA AACCTGTACC ATAACACGGA GTGGACTGAC

451 TGGTCTTGTT ATTGGTGGTC TATACCCTGT TTTCTTGGCT ATACCTGTAA

501 ATGGTGGTCT AGCAGCCAGG TATCAATCAG CTCTGTTACC ACACAAAGGG

551 AACATCTTAA GTTACTGGAT TAGAACTTCT AAGCCTGTCT TTAGAAAGAT

601 GTTATTTCCT ATTTTGCTCC AGACTATGTT TTCAGCATAC CTTGGGTCTG

651 AACAATATAA ACTACTTATA AAGGCCCTTC AGTTATCTGA ACCTGGCAAA

701 GAAATTCACT GATTTTAAAC AAATATGTAA ACAAAAATAA AATGGTAAAA 751 ACAAAAAAAA AAAAAAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 125 bp to 709 bp; peptide length: 195 Category: putative protein

1 MENHKSNNKE NITIVDISRK INQLPEAERN LLENGSVYVG LNAALCGLIA 51 NSLFRRILNV TKARIAAGLP MAGIPFLTTD LTYRCFVSFP LNTGDLDCET 101 CTITRSGLTG LVIGGLYPVF LAIPVNGGLA ARYQSALLPH KGNILSYWIR 151 TSKPVFRKML FPILLQTMFS AYLGSEQYKL LIKALQLSEP GKEIH

BLASTP hits

No BLASTP hits available Alert BLASTP hits for DKFZphutel_24cl9, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphutel_24cl9, frame 2

Report for DKFZphutel_24cl9.2

[LENGTH] 195

[MW] 21527.45

[pi] 9.36

[PROSITE] MYRISTYL 6

[PROSITE] CK2_PHOSPHO_SITE

[PROSITE] PKC_PHOSPHO_SITE

[PROSITE] ASN_GLYCOSYLATION

[KW] TRANSMEMBRANE 1

SEQ MENHKSNNKENITIVDISRKINQLPEAERNLLENGSVYVGLNAALCGLIANSLFRRILNV PRD cccccccccceeeeeehhhhhhccchhhhhhhccccceeeecchhhhhhhhhhhhhhhhh MEM

SEQ TKARIAAGLPMAGIPFLTTDLTYRCFVSFPLNTGDLDCETCTITRSGLTGLVIGGLYPVF PRD hhhhhhhccccccceeeeecccccccccccccccccccccccccccccceeeecccceee MEM MMMMMMMMMMMMMM

SEQ LAIPVNGGLAARYQSALLPHKGNILSYWIRTSKPVFRKMLFPILLQTMFSAYLGSEQYKL PRD eeeccccccchhhhhhccccccceeeeeeecccchhhhhchhhhhhhhhhhhhcchhhhh

MEM MMM

SEQ LIKALQLSEPGKEIH PRD hhhhhhhcccccccc MEM

Prosite for DKFZphutel_J_24cl9.2

PS00001 11->15 ASN_GLYCOSYLATION PDOC00001 PS00001 34->38 ASN_GLYCOSYLATION PDOC00001 PS00001 59->63 ASN_GLYCOSYLATION PDOC00001 PS00005 18->21 PKC_PHOSPHO_SITE PDOC00005 PS00005 82->85 PKC_PHOSPHO_SITE PDOC00005 PS00005 151->154 PKC_PHOSPHO_SITE PDOC00005 PS00006 13->17 CK2_PHOSPHO_SITE PDOC00006 PS00008 40->46 MYRISTYL PDOC00008 PS00008 47->53 MYRISTYL PDOC00008 PS00008 68->74 MYRISTYL PDOC00008 PΞ00008 110->116 MYRISTYL PDOC00008 PS00008 127->133 MYRISTYL PDOC00008 PS00008 142->148 MYRISTYL PDOC00008

(No Pfam data available for DKFZphutel_24cl9.2)

DKFZphutel 24ell

group: intracellular transport and trafficking

DKFZphutel_24ell encodes a novel 226 ammo acid protein, with similarity to human/mouse golgi 4-transmembrane spanning transporter MTP. MTP may function in the transport of nucleosides and/or nucleoside derivatives between the cytosol and the lumen of an intracellular membrane- bound compartment. Thus, the novel protein also seems to be involved in nucleotide sugar transport .

The new protein can find application in modulating the transport of nucleosides and/or nucleoside derivatives between the cytosol and the lumen of an intracellular membrane-bound compartments .

Similarity to 4-TRANSMEMBRANE SPANNING TRANSPORTER MTP complete cDNA, complete eds, EST hits potential start at 184,

TRANSMEMBRANE 4 function in the transport of nucleosides and/or nucleoside derivatives between the cytosol and the lumen of an intracellular membrane-bound compartment⁹

Sequenced by Qiagen

Locus: /map="8"

Insert length: 2005 bp

Poly A stretch at pos. 1988, polyadenylation signal at pos. 1963

1 ACGCGTCCGG CAGAAGCTCG GAGCTCTCGG GGTATCGAGG AGGCAGGCCC

51 GCGGGCGCAC GGGCGAGCGG GCCGGGAGCC GGAGCGGCGG AGGAGCCGGC

101 AGCAGCGGCG CGGCGGGCTC CAGGCGAGGC GGTCGACGCT CCTGAAAACT

151 TGCGCGCGCG CTCGCGCCAC TGCGCCCGGA GCGATGAAGA TGGTCGCGCC

201 CTGGACGCGG TTCTACTCCA ACAGCTGCTG CTTGTGCTGC CATGTCCGCA

251 CCGGCACCAT CCTGCTCGGC GTCTGGTATC TGATCATCAA TGCTGTGGTA

301 CTGTTGATTT TATTGAGTGC CCTGGCTGAT CCGGATCAGT ATAACTTTTC

351 AAGTTCTGAA CTGGGAGGTG ACTTTGAGTT CATGGATGAT GCCAACATGT

401 GCATTGCCAT TGCGATTTCT CTTCTCATGA TCCTGATATG TGCTATGGCT

451 ACTTACGGAG CGTACAAGCA ACGCGCAGCC TGGATCATCC CATTCTTCTG

501 TTACCAGATC TTTGACTTTG CCCTGAACAT GTTGGTTGCA ATCACTGTGC

551 TTATTTATCC AAACTCCATT CAGGAATACA TACGGCAACT GCCTCCTAAT

601 TTTCCCTACA GAGATGATGT CATGTCAGTG AATCCTACCT GTTTGGTCCT

651 TATTATTCTT CTGTTTATTA GCATTATCTT GACTTTTAAG GGTTACTTGA

701 TTAGCTGTGT TTGGAACTGC TACCGATACA TCAATGGTAG GAACTCCTCT

751 GATGTCCTGG TTTATGTTAC CAGCAATGAC ACTACGGTGC TGCTACCCCC

801 GTATGATGAT GCCACTGTGA ATGGTGCTGC CAAGGAGCCA CCGCCACCTT

851 ACGTGTCTGC CTAAGCCTTC AAGTGGGCGG AGCTGAGGGC AGCAGCTTGA

901 CTTTGCAGAC ATCTGAGCAA TAGTTCTGTT ATTTCACTTT TGCCATGAGC

951 CTCTCTGAGC TTGTTTGTTG CTGAAATGCT ACTTTTTAAA ATTTAGATGT

1001 TAGATTGAAA ACTGTAGTTT TCAACATATG CTTTGCTAGA ACACTGTGAT

1051 AGATTAACTG TAGAATTCTT CCTGTACGAT TGGGGATATA ACGGGCTTCA

1101 CTAACCTTCC CTAGGCATTG AAACTTCCCC CAAATCTGAT GGACCTAGAA

1151 GTCTGCTTTT GTACCTGCTG GGCCCCAAAG TTGGGCATTT TTCTCTCTGT

1201 TCCCTCTCTT TTGAAAATGT AAAATAAAAC CAAAAATAGA CAACTTTTTC

1251 TTCAGCCATT CCAGCATAGA GAACAAAACC TTATGGAAAC AGGAATGTCA

1301 ATTGTGTAAT CATTGTTCTA ATTAGGTAAA TAGAAGTCCT TATGTATGTG

1351 TTACAAGAAT TTCCCCCACA ACATCCTTTA TGACTGAAGT TCAATGACAG

1401 TTTGTGTTTG GTGGTAAAGG ATTTTCTCCA TGGCCTGAAT TAAGACCATT

1451 AGAAAGCACC AGGCCGTGGG AGCAGTGACC ATCTACTGAC TGTTCTTGTG

1501 GATCTTGTGT CCAGGGACAT GGGGTGACAT GCCTCGTATG TGTTAGAGGG

1551 TGGAATGGAT GTGTTTGGCG CTGCATGGGA TCTGGTGCCC CTCTTCTCCT

1601 GGATTCACAT CCCCACCCAG GGCCCGCTTT TACTAAGTGT TCTGCCCTAG

1651 ATTGGTTCAA GGAGGTCATC CAACTGACTT TATCAAGTGG AATTGGGATA

1701 TATTTGATAT ACTTCTGCCT AACAACATGG AAAAGGGTTT TCTTTTCCCT

1751 GCAAGCTACA TCCTACTGCT TTGAACTTCC AAGTATGTCT AGTCACCTTT

1801 TAAAATGTAA ACATTTTCAG AAAAATGAGG ATTGCCTTCC TTGTATGCGC

1851 TTTTTACCTT GACTACCTGA ATTGCAAGGG ATTTTTATAT ATTCATATGT

1901 TACAAAGTCA GCAACTCTCC TGTTGGTTCA TTATTGAATG TGCTGTAAAT

1951 TAAGTCGTTT GCAATTAAAA CAAGGTTTGC CCACATCCAA AAAAAAAAAA

2001 AAAAA

BLAST Results

Entry HS012351 from database EMBL: human STS SHGC-31823. Score = 1629, P = 3. 1e-67 , identities = 343/354

Medline entries

96199248-

Identification of a novel membrane transporter associated with intracellular membranes by phenotypic complementation in the yeast Saccharomyces cerevisiae.

Peptide information for frame 1

ORF from 184 bp to 861 bp; peptide length: 226 Category: strong similarity to known protein

1 MKMVAPWTRF YSNSCCLCCH VRTGTILLGV WYLIINAWL LILLSALADP

51 DQYNFSSSEL GGDFEFMDDA NMCIAIAISL LMILICAMAT YGAYKQRAAW

101 IIPFFCYQIF DFALNMLVAI TVLIYPNSIQ EYIRQLPPNF PYRDDVMSVN

151 PTCLVLIILL FISIILTFKG YLIΞCVWNCY RYINGRNSSD VLVYVTΞNDT

201 TVLLPPYDDA TVNGAAKEPP PPYVSA

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_24ell, frame 1

SWISSPROT:MTRP_HUMAN GOLGI 4-TRANSMEMBRANE SPANNING TRANSPORTER MTP (KIAA0108)., N = 1, Score = 551, P = 2.9e-53

SWISSPROT:MTRP_MOUΞE GOLGI 4-TRANSMEMBRANE SPANNING TRANSPORTER MTP., N = 1, Score = 539, P = 5.3e-52

TREMBL :HS304981_1 product: "E3 protein"; Human retmoic acid-mducible E3 protein mRNA, complete eds., N = 1, Score = 127, P = 3.4e-06

>SWISSPROT:MTRP_HUMAN GOLGI 4-TRANSMEMBRANE SPANNING TRANSPORTER MTP (KIAA0108) .

Length = 233

HSPs:

Score = 551 (82.7 bits), Expect = 2.9e-53, P = 2.9e-53 Identities = 102/221 (46%), Positives = 148/221 (66%)

Query: 9 RFYSNSCCLCCHVRTGTILLGVWYLIINAVVLLILLSALADPDQY NFSSSELGGDF- 64

RFYS CC CCHVRTGTI+LG WY+++N ++ ++L + P+ N +G + Sbjct: 13 RFYSTRCCGCCHVRTGTIILGTWYMVVNLLMAILLTVEVTHPNSMPAVNIQYEVIGNYYS 72

Query: 65 -EFMDDANMCIAIAISLLMILICAMATYGAYKQRAAWIIPFFCYQIFDFALNMLVAITVL 123

E M D N C+ A+S+LM +1 +M YGA + W+IPFFCY++FDF L+ LVAI+ L Sbjct: 73 SERMAD-NACVLFAVSVLMFIISSMLVYGAISYQVGWLIPFFCYRLFDFVLSCLVAISSL 131

Query: 124 IYPNSIQEYIRQLPPNFPYRDDVMSVNPTCLVLIILLFISIILTFKGYLISCVWNCYRYI 183

Y I+EY+ QLP +FPY+DD+++++ +CL+ I+L+F ++ + FK YLI+CVWNCY+YI Sbjct: 132 TYLPRIKEYLDQLP-DFPYKDDLLALDSSCLLFIVLVFFALFIIFKAYLINCVWNCYKYI 190

Query: 184 NGRNSSDVLVYVTSN-DTTVLLPPYDDATVNGAAKEPPPPYVSA 226

N RN ++ VY +LP Y+ A V KEPPPPY+ A

Sbjct: 191 NNRNVPEIAVYPAFEAPPQYVLPTYEMA-VKMPEKEPPPPYLPA 233

Pedant information for DKFZphutel_24ell, frame 1

Report for DKFZphutel_24ell .1

[LENGTH] 226

[MW] 25419.11 [pi] 4.65

[HOMOL] SWISSPROT:MTRP HUMAN GOLGI 4-TRANSMEMBRANE SPANNING TRANSPORTER MTP (KIAA0108)

5e-40

[PROSITE] CK2_PHOSPHO_SITE

[PROSITE] TYR_PHOSPHO_SITE

[PROSITE] PKC_PHOSPHO_SITE

[PROSITE] ASN_GLYCOSYLATION

[KW] SIGNAL_PEPTIDE 49

[KW] TRANSMEMBRANE 2

[KW] LOW COMPLEXITY 20.80 %

SEQ MKMVAPWTRFYSNSCCLCCHVRTGTILLGVWYLIINAVVLLILLSALADPDQYNFSSSEL SEG xxxxxxxxxxxxxxxx PRD ccceeeeeeecccceeeeeeeeccceeecceeehhhhhhhhhhhhhhcccccceeecccc MEM

SEQ GGDFEFMDDANMCIAIAISLLMILICAMATYGAYKQRAAWIIPFFCYQIFDFALNMLVAI

SEG xxxxxxxxxxxxxxxxxx

PRD ccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhh

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ TVLIYPNSIQEYIRQLPPNFPYRDDVMSVNPTCLVLIILLFISIILTFKGYLISCVWNCY

SEG xxxxxxxxxxxxx

PRD hhhcccchhhhhhhhcccccccccceeeeccccceeehhhhhhhhhhhhhheeeeeeeee

MEM MMMMMM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM....

SEQ RYINGRNSSDVLVYVTSNDTTVLLPPYDDATVNGAAKEPPPPYVSA SEG PRD eecccccccceeeeeecccccccccccccccccccccccccccccc MEM

Prosite for DKFZphutel_24ell .1

PS00001 54->58 ASN GLYCOSYLATION PDOC00001

PS00001 187->191 ASN GLYCOSYLATION PDOC00001

PS00001 198->202 ASN GLYCOSYLATION PDOC00001

PS00005 167->170 PKC PHOSPHO SITE PDOC00005

PS00006 56->60 CK2 PHOSPHO SITE PDOC00006

PS00006 128->132 CK2 PHOSPHO SITE PDOC00006

PS00006 196->200 CK2 PHOSPHO SITE PDOC00006

PS00007 186->195 TYR PHOSPHO SITE PDOC00007

(No Pfam data available for DKFZphutel_24ell .1)

DKFZphutel_24j6

group: cell structure and motility

DKFZphutesl_24j 6 encodes a novel 571 amino acid protein with strong similarity to rat cell adhesion regulator (CARD .

The novel protein is very similar to Carl and thus seems to be involved in regulation cell- cell adhesion. It contains a RGD cell attachment site.

The new protein can find application in modulation of cell-cell-adhesion. strong similarity to rat CAR1 A. thaliana T19C21.5 complete cDNA, complete eds, EST hits potential frame shift at Bp 1241 according to CAR1 but frame shift might be m CAR1 sequence¹

ESTs T73366 AA362984 confirm this sequence

Sequenced by Qiagen

Locus: /map="939.9 cR from top of Chr2 linkage group"

Insert length: 3333 bp

Poly A stretch at pos. 3316, no polyadenylation signal found

1 ACGCGTCCGA GCTGGCTCAG GGCGTCCGCT AGGCTCGGAC GACCTGCTGA 51 GCCTCCCAAA CCGCTTCCAT AAGGCTTTGC CTTTCCAACT TCAGCTACAG

101 TGTTAGCTAA GTTTGGAAAG AAGGAAAAAA GAAAATCCCT GGGCCCCTTT

151 TCTTTTGTTC TTTGCCAAAG TCGTCGTTGT AGTCTTTTTG CCCAAGGCTG

201 TTGTGTTTTT AGAGGTGCTA TCTCCAGTTC CTTGCACTCC TGTTAACAAG

251 CACCTCAGCG AGAGCAGCAG CAGCGATAGC AGCCGCAGAA GAGCCAGCGG

301 GGTCGCCTAG TGTCATGACC AGGGCGGGAG ATCACAACCG CCAGAGAGGA

351 TGCTGTGGAT CCTTGGCCGA CTACCTGACC TCTGCAAAAT TCCTTCTCTA

401 CCTTGGTCAT TCTCTCTCTA CTTGGGGAGA TCGGATGTGG CACTTTGCGG

451 TGTCTGTGTT TCTGGTAGAG CTCTATGGAA ACAGCCTCCT TTTGACAGCA

501 GTCTACGGGC TGGTGGTGGC AGGGTCTGTT CTGGTCCTGG GAGCCATCAT

551 CGGTGACTGG GTGGACAAGA ATGCTAGACT TAAAGTGGCC CAGACCTCGC

601 TGGTGGTACA GAATGTTTCA GTCATCCTGT GTGGAATCAT CCTGATGATG

651 GTTTTCTTAC ATAAACATGA GCTTCTGACC ATGTACCATG GATGGGTTCT

701 CACTTCCTGC TATATCCTGA TCATCACTAT TGCAAATATT GCAAATTTGG

751 CCAGTACTGC TACTGCAATC ACAATCCAAA GGGATTGGAT TGTTGTTGTT

801 GCAGGAGAAG ACAGAAGCAA ACTAGCAAAT ATGAATGCCA CAATACGAAG

851 GATTGACCAG TTAACCAACA TCTTAGCCCC CATGGCTGTT GGCCAGATTA

901 TGACATTTGG CTCCCCAGTC ATCGGCTGTG GCTTTATTTC GGGATGGAAC

951 TTGGTATCCA TGTGCGTGGA GTACGTCCTG CTCTGGAAGG TTTACCAGAA 1001 AACCCCAGCT CTAGCTGTGA AAGCTGGTCT TAAAGAAGAG GAAACTGAAT 1051 TGAAACAGCT GAATTTACAC AAAGATACTG AGCCAAAACC CCTGGAGGGA 1101 ACTCATCTAA TGGGTGTGAA AGACTCTAAC ATCCATGAGC TTGAACATGA 1151 GCAAGAGCCT ACTTGTGCCT CCCAGATGGC TGAGCCCTTC CGTACCTTCC 1201 GAGATGGATG GGTCTCCTAC TACAACCAGC CTGTGTTTCT GGCTGGCATG 1251 GGTCTTGCTT TCCTTTATAT GACTGTCCTG GGCTTTGACT GCATCACCAC 1301 AGGGTACGCC TACACTCAGG GACTGAGTGG TTCCATCCTC AGTATTTTGA 1351 TGGGAGCATC AGCTATAACT GGAATAATGG GAACTGTAGC TTTTACTTGG 1401 CTACGTCGAA AATGTGGTTT GGTTCGGACA GGTCTGATCT CAGGATTGGC 1451 ACAGCTTTCC TGTTTGATCT TGTGTGTGAT CTCTGTATTC ATGCCTGGAA 1501 GCCCCCTGGA CTTGTCCGTT TCTCCTTTTG AAGATATCCG ATCAAGGTTC 1551 ATTCAAGGAG AGTCAATTAC ACCTACCAAG ATACCTGAAA TTACAACTGA 1601 AATATACATG TCTAATGGGT CTAATTCTGC TAATATTGTC CCGGAGACAA 1651 GTCCTGAATC TGTGCCCATA ATCTCTGTCA GTCTGCTGTT TGCAGGCGTC 1701 ATTGCTGCTA GAATCGGTCT TTGGTCCTTT GATTTAACTG TGACACAGTT 1751 GCTGCAAGAA AATGTAATTG AATCTGAAAG AGGCATTATA AATGGTGTAC 1801 AGAACTCCAT GAACTATCTT CTTGATCTTC TGCATTTCAT CATGGTCATC 1851 CTGGCTCCAA ATCCTGAAGC TTTTGGCTTG CTCGTATTGA TTTCAGTCTC 1901 CTTTGTGGCA ATGGGCCACA TTATGTATTT CCGATTTGCC CAAAATACTC 1951 TGGGAAACAA GCTCTTTGCT TGCGGTCCTG ATGCAAAAGA AGTTAGGAAG 2001 GAAAATCAAG CAAATACATC TGTTGTTTGA GACAGTTTAA CTGTTGCTAT 2051 CCTGTTACTA GATTATATAG AGCACATGTG CTTATTTTGT ACTGCAGAAT 2101 TCCAATAAAT GGCTGGGTGT TTTGCTCTGT TTTTACCACA GCTGTGCCTT 2151 GAGAACTAAA AGCTGTTTAG GAAACCTAAG TCAGCAGAAA TTAACTGATT 2201 AATTTCCCTT ATGTTGAGGC ATGGAAAAAA AATTGGAAAA GAAAAACTCA 2251 GTTTAAATAC GGAGACTATA ATGATAACAC TGAATTCCCC TATTTCTCAT 2301 GAGTAGATAC AATCTTACGT AAAAGAGTGG TTAGTCACGT GAATTCAGTT 2351 ATCATTTGAC AGATTCTTAT CTGTACTAGA ATTCAGATAT GTCAGTTTTC 2401 TGCAAAACTC ACTCTTGTTC AAGACTAGCT AATTTATTTT TTTGCATCTT 2451 AGTTATTTTT AAAAACAAAT TCTTCAAGTA TGAAGACTAA ATTTTGATAA 2501 CTAATATTAT CCTTATTGAT CCTATTGATC TTAAGGTATT TACATGTATG 2551 TGGAAAAACA AAACACTTAA CTAGAATTCT CTAATAAGGT TTATGGTTTA 2601 GCTTAAAGAG CACCTTTGTA TTTTTATTAT CAGATGGGGC AACATATTGT 2651 ATGAAGCATA TGTAGCACTT CACAGCATGG TTATCATGTA AGCTGCAGGT 2701 AGAAGCAAAG CTGTAAAGTA GATTTATCAC ACAATGACTG CATACAGACT 2751 TCAAATATGT CAATAGTTTG GTCATAGAAC CTAGAAGCCA AAAGCCACAC 2801 AGAAGGGCAA GAATCCCAAT TTAACTCATG TTATCATCAT TAGTGATCTG 2851 TGTTGTAGAA CATGAGGGTG TAAGCCTTCA GCCTGGCAAG TTACATGTAG 2901 AAAGCCCACA CTTGTGAAGG TTTTGTTTTA CAAATCACTT GATTTAACAC 2951 ACTCAGGTAG AATATTTTTA TTTTTACTGT TTTATACCCA GAAGTTATTT 3001 CTACATTGTT CTACAGCAAG AATATTCATA AAAGTATCCC TTTCAAATGC 3051 CTTTGAGAAG AATAGAAGAA AAAAAGTTTG TATATATTTT AAAAAATTGT 3101 TTTAAAAGTC AGTTTGCAAC ATGTCTGTAC CAAGATGGTA CTTTGCCTTA 3151 ACCGTTTATA TGCACTTTCA TGGAGACTGC AATACGTTGC TATGAGCACT 3201 TTCTTTATCC TTGGAGTTTA ATCCTTTGCT TCATCTTTCT ACAGTATGAC 3251 ATAATGATTT GCTATGTTGT AAAATCTTTG TAAAAAATTT CTATATAAAA 3301 ATATTTTGAA AATCTTAAAA AAAAAAAAAA AAA

BLAST Results

Entry HS389210 from database EMBL: human STS SHGC-10164. Score = 1592, P = 1.5e-64, identities = 346/364

Entry HS933343 from database EMBL: human STS WI-16551. Score = 1193, P = 5.7e-46, identities = 241/244

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 315 bp to 2027 bp; peptide length: 571 Category: strong similarity to known protein

1 MTRAGDHNRQ RGCCGSLADY LTSAKFLLYL GHSLSTWGDR MWHFAVSVFL

51 VELYGNSLLL TAVYGLVVAG SVLVLGAIIG DWVDKNARLK VAQTSLVVQN

101 VSVILCGIIL MMVFLHKHEL LTMYHGWVLT SCYILIITIA NIANLASTAT

151 AITIQRDWIV VVAGEDRSKL ANMNATIRRI DQLTNILAPM AVGQIMTFGS

201 PVIGCGFISG WNLVSMCVEY VLLWKVYQKT PALAVKAGLK EEETELKQLN

251 LHKDTEPKPL EGTHLMGVKD SNIHELEHEQ EPTCASQMAE PFRTFRDGWV

301 SYYNQPVFLA GMGLAFLYMT VLGFDCITTG YAYTQGLSGS ILSILMGASA

351 ITGIMGTVAF TWLRRKCGLV RTGLISGLAQ LSCLILCVIS VFMPGSPLDL

401 SVSPFEDIRS RFIQGESITP TKIPEITTEI YMSNGSNSAN IVPETSPESV

451 PIISVSLLFA GVIAARIGLW SFDLTVTQLL QENVIESERG IINGVQNSMN

501 YLLDLLHFIM VILAPNPEAF GLLVLISVSF VAMGHIMYFR FAQNTLGNKL

551 FACGPDAKEV RKENQANTSV V

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_24j 6, frame 3

TREMBLNEW :U76714_1 gene: "CARl"; product: "cell adhesion regulator"; Rattus norvegicus cell adhesion regulator (CARl) mRNA, complete eds., N = 1, Score = 1472, P = 7.2e-151

TREMBL:AC004683_5 gene: "T19C21.5"; Arabidopsis thaliana chromosome II BAC T19C21 genomic sequence, complete sequence., N = 2, Score = 437, P = 2.8e-60

TREMBL: AF039046_2 gene: "R09B5.4"; Caenorhabditis elegans cosmid R09B5., N = 2, Score = 323, P = 1.5e-43

>TREMBLNEW:U76714_1 gene: "CARl"; product: "cell adhesion regulator";

Rattus norvegicus cell adhesion regulator (CARl) mRNA, complete eds. Length = 405 HSPs:

Score = 1472 (220.9 bits), Expect = 7.2e-151, P = 7.2e-151 Identities = 288/319 (90%), Positives = 297/319 (93%)

Query: 1 MTRAGDHNRQRGCCGSLADYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLL 60

MT++ D Q GCCGΞLA+YLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLL Sbjct: 1 MTKSRDQTHQEGCCGΞLANYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLL 60

Query: 61 TAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKHEL 120

TAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHK+EL Sbjct: 61 TAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKNEL 120

Query: 121 LTMYHGWVLTSCYILIITIANIANLASTATAITIQRDWIVVVAGEDRSKLANMNATIRRI 180

L MYHGWVLT CYILIITIANIANLASTATAITIQRDWIVVVAGE+RS+LA+MNATIRRI Sbjct: 121 LNMYHGWVLTVCYILIITIANIANLASTATAITIQRDWIVVVAGENRSRLADMNATIRRI 180

Query: 181 DQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYVLLWKVYQKTPALAVKAGLK 240

DQLTNILAPMAVGQIMTFGSPVIGCGFIΞGWNLVSMCVEY LLWKVYQKTPALAVKA LK Sbjct: 181 DQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYFLLWKVYQKTPALAVKAALK 240

Query: 241 EEETELKQLNLHKDTEPKPLEGTHLMGVKDSNIHELEHEQEPTCASQMAEPFRTFRDGWV 300

EE+ELKQL KDTEPKPLEGTHLMG KDSNI ELE EQEPTCASQ+AEPFRTFRDGWV Sbjct: 241 VEESELKQLTSPKDTEPKPLEGTHLMGEKDSNIRELECEQEPTCASQIAEPFRTFRDGWV 300

Query: 301 SYYNQPVFLAGMGLAF-LY 318

SYYNQPVFL G F LY Sbjct: 301 SYYNQPVFLGWHGPGFPLY 319

Pedant information for DKFZphutel_24 6, frame 3

Report for DKFZphutel_24j 6.3

[LENGTH] 571 [MW] 62542.72 [pi] 6.08 [HOMOL] TREMBL :U76714_1 gene: "CARl"; product: "cell adhesion regulator"; Rattus norvegicus cell adhesion regulator (CARl) mRNA, complete eds. le-141

[BLOCKS] BL00341D

[PROSITE] MYRISTYL 15

[PROSITE] MITOCH_CARRIER 1

[PROSITE] CK2_PHOSPHO_SITE 6

[PROSITE] PROKAR_LIPOPROTEIN 1

[PROSITE] PKC_PHOSPHO_SITE 4

[PROSITE] ASN_GLYCOSYLATION 4

[PFAM] Lammm B (Domain IV)

[KW] TRANSMEMBRANE 4

[KW] LOW COMPLEXITY 8.76 %

SEQ MTRAGDHNRQRGCCGSLADYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLL

SEG

PRD ccccccccccccccccchhhhhhhheeeeccceeecccchhhhhhhhheeeeecccccee

MEM MMMMMMMMMMMMM

SEQ TAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKHEL

SEG . xxxxxxxxxxxxxxxx

PRD ehhhhhhhccceeeeccccccchhhhhhhhhhhhheeeccchhhhhhhhhhhhhhhhhhh

MEM MMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ LTMYHGWVLTSCYILIITIANIANLASTATAITIQRDWIVVVAGEDRSKLANMNATIRRI SEG xxxxxxxxxxxxxxxxxxxxx PRD hhcccccchhhhhhhhhhhhhhhhhhhhhheeeeccceeeeeeccccchhhhhhhhhhhh MEM MMMMMMM

SEQ DQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYVLLWKVYQKTPALAVKAGLK SEG PRD hhhhhhccceeeceeeeeecceeeeeeeeccchhhhhhhhhhhhhhhcccchhhhhhhhh MEM

SEQ EEETELKQLNLHKDTEPKPLEGTHLMGVKDSNIHELEHEQEPTCASQMAEPFRTFRDGWV

SEG

PRD hhhhhhhhhhccccccccccceeeeeecccccccccccccccccccccccccccccccee

MEM

SEQ SYYNQPVFLAGMGLAFLYMTVLGFDCITTGYAYTQGLSGSILSILMGASAITGIMGTVAF

SEG

PRD eeecceeeecccchhhhhhcccccceeeeeeeeccccceeeeeeecccceeeeehhhhhh MEM

SEQ TWLRRKCGLVRTGLISGLAQLSCLILCVISVFMPGSPLDLSVSPFEDIRSRFIQGESITP SEG XXX PRD hhhhhhccccccccchhhhhhhhhhhhhhhhcccccccccccccchhhhhhccccccccc MEM

SEQ TKIPEITTEIYMSNGSNΞANIVPETSPESVPIISVSLLFAGVIAARIGLWΞFDLTVTQLL

SEG xxxxxxxxxx

PRD ccccccceeeeecccccccccccccccccceeeeeehhhhhhhhhhcccchhhhhhhhhh

MEM MMMMMMMMMMMMMMMMMMMMMMMM

SEQ QENVIEΞERGIINGVQNΞMNYLLDLLHFIMVILAPNPEAFGLLVLISVSFVAMGHIMYFR SEG PRD hhhhhccccceeeecccchhhhhhhhhhheeeeeccccccceeeeeeeeccccccceeee MEM MMMMMMMMMMMMMMMMMMMMMMMMMMM ...

SEQ FAQNTLGNKLFACGPDAKEVRKENQANTSVV SEG PRD eecccccceeeeccccchhhhhhhhcccccc MEM

Prosite for DKFZphutel_24j 6.3

PS00001 100->104 ASN_GLYCOSYLATION PDOC00001

PS00001 174->178 ASN_GLYCOSYLATION PDOC00001

PS00001 434->438 ASN_GLYCOSYLATION PDOC00001

PS00001 567->571 ASN_GLYCOSYLATION PDOC00001

PS00005 23->26 PKC_PHOSPHO_SITE PDOC00005

PS00005 176->179 PKC_PHOSPHO_SITE PDOC00005

PS00005 294->297 PKC_PHOSPHO_SITE PDOC00005

PS00005 487->490 PKC_PHOSPHO_SITE PDOC00005

PS00006 16->20 CK2_PHOSPHO_SITE PDOC00006

PS00006 36->40 CK2_PHOSPHO_SITE PDOC00006

PS00006 294->298 CK2_PHOSPHO_SITE PDOC00006

PS00006 396->400 CK2_PHOSPHO_SITE PDOC00006

PS00006 403->407 CK2_PHOSPHO_SITE PDOC00006

PS00006 445->449 CK2_PHOSPHO_SITE PDOC00006

PS00008 12->18 MYRISTYL PDOC00008

PS00008 65->71 MYRISTYL PDOC00008

PS00008 76->82 MYRISTYL PDOC00008

PS00008 193->199 MYRISTYL PDOC00008

PS00008 267->273 MYRISTYL PDOC00008

PS00008 311->317 MYRISTYL PDOC00008

PS00008 336->342 MYRISTYL PDOC00008

PS00008 339->345 MYRISTYL PDOC00008

PS00008 353->359 MYRISTYL PDOC00008

PS00008 368->374 MYRISTYL PDOC00008

PS00008 373->379 MYRISTYL PDOC00008

PS00008 435->441 MYRISTYL PDOC00008

PS00008 461->467 MYRISTYL PDOC00008

PS00008 490->496 MYRISTYL PDOC00008

PS00008 494->500 MYRISTYL PDOC00008

PS00013 122->133 PROKAR_LIPOPROTEIN PDOC00013

PS00215 404->414 MITOCH CARRIER PDOC00189

Pfam for DKFZphutel_24j 6.3

HMM_NAME Lammm B (Domain IV)

HMM *YWRlPERFLGDQvTsYGGkLe*

Y+R + LG+++ + G + + Query 538 YFRFAQNTLGNKLFACGPDAK 558 DKFZphutel_2h3

group: differentiation/development

DKFZphutel_2h3 encodes a novel 267 amino acid protein, with similarity to ITM2 (integral membrane protein 2) of chicken and mouse.

The novel protein contains a prenyl group binding site (CAAX box) and seems to be post- translationally modified by the attachment of either a farnesyl or a geranyl-geranyl group. The similar gallus G. protein E25 a marker for chondro-osteogemc differentiation.

The new protein can find application as a useful marker for chondro-osteogemc cell differentiation and for the modulation of chondro-osteogemc cell differentiation. strong similarity to mouse E25 and gallus E3-16 complete cDNA, EST hits complete eds according to E25 start at Bp 56 putative transmembrane protein (1 TM)

Sequenced by AGOWA

Locus : unknown

Insert length: 2033 bp

Poly A stretch at pos. 2007, polyadenylation signal at pos. 1986

1 GGACCGAGGC TGCACCGGCA GAGGCTGCGG GGCGGACGCG CGGGCCGGCG

51 CAGCCATGGT GAAGATTAGC TTCCAGCCCG CCGTGGCTGG CATCAAGGGC

101 GACAAGGCTG ACAAGGCGTC GGCGTCGGCC CCTGCGCCGG CCTCGGCCAC

151 CGAGATCCTG CTGACGCCGG CTAGGGAGGA GCAGCCCCCA CAACATCGAT

201 CCAAGAGGGG GAGCTCAGTG GGCGGCGTGT GCTACCTGTC GATGGGCATG

251 GTCGTGCTGC TCATGGGCCT CGTGTTCGCC TCTGTCTACA TCTACAGATA

301 CTTCTTTCTT GCACAGCTGG CCCGAGATAA CTTCTTCCGC TGTGGTGTGC

351 TGTATGAGGA CTCCCTGTCC TCCCAGGTCC GGACTCAGAT GGAGCTGGAA

401 GAGGATGTGA AAATCTACCT CGACGAGAAC TACGAGCGCA TCAACGTGCC

451 TGTGCCCCAG TTTGGCGGCG GTGACCCTGC AGACATCATC CATGACTTCC

501 AGCGGGGTCT GACTGCGTAC CATGATATCT CCCTGGACAA GTGCTATGTC

551 ATCGAACTCA ACACCACCAT TGTGCTGCCC CCTCGCAACT TCTGGGAGCT

601 CCTCATGAAC GTGAAGAGGG GGACCTACCT GCCGCAGACG TACATCATCC

651 AGGAGGAGAT GGTGGTCACG GAGCATGTCA GTGACAAGGA GGCCCTGGGG

701 TCCTTCATCT ACCACCTGTG CAACGGGAAA GACACCTACC GGCTCCGGCG

751 CCGGGCAACG CGGAGGCGGA TCAACAAGCG TGGGGCCAAG AACTGCAATG

801 CCATCCGCCA CTTCGAGAAC ACCTTCGTGG TGGAGACGCT CATCTGCGGG

851 GTGGTGTGAG GCCCTCCTCC CCCAGAACCC CCTGCCGTGT TCCTCTTTTC

901 TTCTTTCCAG CTGCTCTCTG GCCCTCCTCC TTCCCCCTGC TTAGCTTGTA

951 CTTTGGACGC GTTTCTATAG AGGTGACATG TCTCTCCATT CCTCTCCAAC

1001 CCTGCCCACC TCCCTGTACC AGAGCTGTGA TCTCTCGGTG GGGGGCCCAT

1051 CTCTGCTGAC CTGGGTGTGG CGGAGGGAGA GGCGATGCTG CAAAGTGTTT

1101 TCTGTGTCCC ACTGTCTTGA AGCTGGGCCT GCCAAAGCCT GGGCCCACAG

1151 CTGCACCGGC AGCCCAAGGG GAAGGACCGG TTGGGGGAGC CGGGCATGTG

1201 AGGCCCTGGG CAAGGGGATG GGGCTGTGGG GGCGGGGCGG CATGGGCTTC

1251 AGAAGTATCT GCACAATTAG AAAAGTCCTC AGAAGCTTTT TCTTGGAGGG

1301 TACACTTTCT TCACTGTCCC TATTCCTAGA CCTGGGGCTT GAGCTGAGGA

1351 TGGGACGATG TGCCCAGGGA GGGACCCACC AGAGCACAAG AGAAGGTGGC

1401 TACCTGGGGG TGTCCCAGGG ACTCTGTCAG TGCCTTCAGC CCACCAGCAG

1451 GAGCTTGGAG TTTGGGGAGT GGGGATGAGT CCGTCAAGCA CAACTGTTCT

1501 CTGAGTGGAA CCAAAGAAGC AAGGAGCTAG GACCCCCAGT CCTGCCCCCC

1551 AGGAGCACAA GCAGGGTCCC CTCAGTCAAG GCAGTGGGAT GGGCGGCTGA

1601 GGAACGGGGC AGGCAAGGTC ACTGCTCAGT CACGTCCACG GGGGACGAGC

1651 CGTGGGTTCT GCTGAGTAGG TGGAGCTCAT TGCTTTCTCC AAGCTTGGAA

1701 CTGTTTTGAA AGATAACACA GAGGGAAAGG GAGAGCCACC TGGTACTTGT

1751 CCACCCTGCC TCCTCTGTTC TGAAATTCCA TCCCCCTCAG CTTAGGGGAA

1801 TGCACCTTTT TCCCTTTCCT TCTCACTTTT GCATGTTTTT ACTGATCATT

1851 CGATATGCTA ACCGTTCTCA GCCCTGAGCC TTGGAGAGGA GGGCTGTAAC

1901 GCCTTCAGTC AGTCTCTGGG GATGAAACTC TTAAATGCTT TGTATATTTT

1951 CTCAATTAGA TCTCTTTTCA GAAGTGTCTA TAGAACAATA AAAATCTTTT

2001 ACTTCTGAAA AAAAAAAAAA AAAAGGGCGG CCG

BLAST Results

Entry B64417 from database EMBL:

CIT-HSP-2023A7.TR CIT-HSP Homo sapiens genomic clone 2023A7.

Length = 715

Plus Strand HSPs : Score = 1546 (232.0 bits), Expect = 7.8e-64, P = 7.8e-64 Identities = 310/311 (99%)

Medline entries

96325063:

Isolation of markers for chondro-osteogemc differentiation using cDNA library subtraction.

Molecular cloning and characterization of a gene belonging to a novel multigene family of integral membrane proteins .

Peptide information for frame 2

ORF from 56 bp to 856 bp; peptide length: 267 Category: strong similarity to known protein

1 MVKISFQPAV AGIKGDKADK ASASAPAPAS ATEILLTPAR EEQPPQHRSK

51 RGSSVGGVCY LSMGMVVLLM GLVFASVYIY RYFFLAQLAR DNFFRCGVLY

101 EDSLSSQVRT QMELEEDVKI YLDENYERIN VPVPQFGGGD PADIIHDFQR

151 GLTAYHDISL DKCYVIELNT TIVLPPRNFW ELLMNVKRGT YLPQTYIIQE

201 EMVVTEHVSD KEALGSFIYH LCNGKDTYRL RRRATRRRIN KRGAKNCNAI

251 RHFENTFVVE TLICGVV

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphutel_2h3, frame 2

SWISSNEW:ITMB_CHICK INTEGRAL MEMBRANE PROTEIN 2B (TRANSMEMBRANE PROTEIN E3-16)., N = 1, Score = 573, P = 1.3e-55

SWISSNEW:ITMB_MOUSE INTEGRAL MEMBRANE PROTEIN 2B (E25B PROTEIN)., N = 1, Score = 560, P = 3.2e-54

SWISSNEW:ITMA_HUMAN INTEGRAL MEMBRANE PROTEIN 2A (E25 PROTEIN)., N = 1, Score = 456, P = 3.3e-43

>SWISSNEW:ITMB_CHICK INTEGRAL MEMBRANE PROTEIN 2B (TRANSMEMBRANE PROTEIN E3-16) .

Length = 262

HSPs:

Score = 573 (86.0 bits), Expect = 1.3e-55, P = 1.3e-55 Identities = 117/264 (44%), Positives = 172/264 (65%)

Query: 1 MVKISFQPAVAGIKGDKADKASASAPAPASATEILLTPAREEQPPQHRSKRGSSVGGVCY 60

MVK+SF A+A + A+K ++ ++L+ P ++P G Sbjct: 1 MVKVSFNSALA—HKEAANKEEENS QVLILPPDAKEPEDVVVPAGHKRAWCWC 51

Query: 61 LSMGMVVLLMGLVFASVYIYRYFFLAQLARDNFFRCGVLY-EDSLS ΞQVRTQM-- 112

+ G+ +L G++ Y+Y+YF Q + CG+ Y ED LS +Q+++ Sbjct: 52 MCFGLAFMLAGVILGGAYLYKYFAFQQ GGVYFCGIKYIEDGLSLPESGAQLKSARYH 108

Query: 113 ELEEDVKIYLDENYERINVPVPQFGGGDPADIIHDFQRGLTAYHDISLDKCYVIELNTTI 172

+E++++I +E+ E I+VPVP+F DPADI+HDF R LTAY D+SLDKCYVI LNT++ Sbjct: 109 TIEQNIQILEEEDVEFISVPVPEFADSDPADIVHDFHRRLTAYLDLSLDKCYVIPLNTSV 168

Query: 173 VLPPRNFWELLMNVKRGTYLPQTYIIQEEMVVTEHVSDKEALGSFIYHLCNGKDTYRLRR 232

V+PP+NF ELL+N+K GTYLPQ+Y+I E+M+VT+ + + + LG FIY LC GK+TY+L+R Sbjct: 169 VMPPKNFLELLINIKAGTYLPQSYLIHEQMIVTDRIENVDQLGFFIYRLCRGKETYKLQR 228

Query: 233 RATRRRINKRGAKNCNAIRHFENTFVVETLIC 264

+ + I KR A NC IRHFEN F +ETLIC Sbjct: 229 KEAMKGIQKREAVNCRKIRHFENRFAMETLIC 260

Pedant information for DKFZphutel_2h3, frame 2 Report for DKFZphutel_2h3.2

[LENGTH] 267

[MW] 30253.96

[pi] 8.16

[HOMOL] SWISSNEW:ITMB_CHICK INTEGRAL MEMBRANE PROTEIN 2B (TRANSMEMBRANE PROTEIN E3-16) le-49

[PROSITE] MYRISTYL 4

[PROSITE) PRENYLATION 1

[PROSITE] CAMP_PHOSPHO_ΞITE 3

[PROSITE] CK2_PHOSPHO_SITE 3

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 4

[PROSITE] ASN_GLYCOSYLATION 1

[KW] TRANSMEMBRANE 1

[KW] LOW COMPLEXITY 15.36 %

SEQ MVKISFQPAVAGIKGDKADKASASAPAPASATEILLTPAREEQPPQHRSKRGSSVGGVCY

SEG xxxxxxxxxxxxxxxx

PRD ccccccccchhhhhhhhhhhhhhhhhccccccceeecccccccccccccccccccccchh

MEM MMMM

SEQ LSMGMVVLLMGLVFASVYIYRYFFLAQLARDNFFRCGVLYEDSLSSQVRTQMELEEDVKI

SEG .. xxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhcchhhhhhhhhhccceeeeeecccccccchhhhhhhhhhhhh

MEM MMMMMMMMMMMMMMMMMMMMMMMM

SEQ YLDENYERINVPVPQFGGGDPADIIHDFQRGLTAYHDISLDKCYVIELNTTIVLPPRNFW SEG PRD hhcccceeeeccccccccccccchhhhhhhhhhhhhhhcccceeeeeccceeecccchhh MEM

SEQ ELLMNVKRGTYLPQTYIIQEEMVVTEHVSDKEALGSFIYHLCNGKDTYRLRRRATRRRIN SEG xxxxxxxxxxxx PRD hhhhhhcccccccceeeeehhhhhhhccccchhhhhheeeccccchhhhhhhhhhhhhhh MEM

SEQ KRGAKNCNAIRHFENTFVVETLICGVV SEG xx PRD hhhhccceeeecccchhhhhheeeccc MEM

Prosite for DKFZphutel_2h3.2

PS00001 169->173 ASN_GLYCOSYLATION PDOC00001 PS00004 50->54 CAMP_PHOSPHO_SITE PDOC00004 PS00004 187-M91 CAMP_PHOSPHO_SITE PDOC00004 PS00004 232->236 CAMP_PHOSPHO_SITE PDOC00004 PS00005 49->52 PKC_PHOSPHO_SITE PDOC00005 PS00005 209->212 PKC_PHOSPHO_SITE PDOC00005 PS00005 227->230 PKC_PHOSPHO_SITE PDOC00005 PS00005 235->238 PKC_PHOSPHO_SITE PDOC00005 PS00006 30->34 CK2_PHOSPHO_SITE PDOC00006 PS00006 110->114 CK2_PHOSPHO_SITE PDOC00006 PS00006 209->213 CK2_PHOSPHO_SITE PDOC00006 PS00007 119->127 TYR_PHOSPHO_SITE PDOC00007 PS00008 52->58 MYRISTYL PDOC00008 PS00008 71->77 MYRISTYL PDOC00008 PS00008 138->144 MYRISTYL PDOC00008 PS00008 243->249 MYRISTYL PDOC00008 PS00294 264->268 PRENYLATION PDOC00266

(No Pfam data available for DKFZphutel 2h3.2) DKFZphmcfl lall

group: transmembrane protein

DKFZphmcfl_lall encodes a novel 393 ammo acid protein with weak similarity to S. pombe SPBC29A3_3 protein and S. cerevisiae putative membrane protein YDR255c.

The novel protein contains 1 transmembrane region.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of mammary carcinoma- specific genes and as a new marker for mammary carcinoma cells. similarity to YDR255c and SPBC29A3.03c membrane regions : 1

Summary DKFZphmcfl_lall encodes a novel 393 amino acid protein, with similarity to YDR255c and SPBC29A3.03c . similarity to YDR255C and SPBC29A3.03c complete cDNA, complete eds, EST hits potential start at Bp 110 matches kozak consensus

Sequenced by DKFZ

Locus: /map="542.7 cR from top of Chr5 linkage group"

Insert length: 1819 bp

Poly A stretch at pos. 1808, no polyadenylation signal found

1 CCCGGCCCAG CCCCCGAAGA GCCGCCTCAG CCGGGGGGAG TTGCTCGGAC 51 TCAAACGTCC AGTCCTCGTG CGACCGCGCT GGGTCGGAAG TGAGCAGGCT

101 GAGGCCACCA TGGAGCAGTG TGCGTGCGTG GAGAGAGAGC TGGACAAGGT

151 CCTGCAGAAG TTCCTGACCT ACGGGCAGCA CTGTGAGCGG AGCCTGGAGG

201 AGCTGCTGCA CTACGTGGGC CAGCTGCGGG CTGAGCTGGC CAGCGCAGCC

251 CTCCAGGGGA CCCCTCTCTC AGCCACCCTC TCTCTGGTGA TGTCACAGTG

301 CTGCCGGAAG ATCAAAGATA CGGTGCAGAA ACTGGCTTCG GACCATAAGG

351 ACATTCACAG CAGTGTATCC CGAGTGGGCA AAGCCATTGA CAGGAACTTC

401 GACTCTGAGA TCTGTGGTGT TGTGTCAGAT GCGGTGTGGG ACGCGCGGGA

451 ACAGCAGCAG CAGATCCTGC AGATGGCCAT CGTGGAACAC CTGTATCAGC

501 AGGGCATGCT CAGCGTGGCC GAGGAGCTGT GCCAGGAATC AACGCTGAAT

551 GTGGACTTGG ATTTCAAGCA GCCTTTCCTA GAGTTGAATC GAATCCTGGA

601 AGCCCTGCAC GAACAAGACC TGGGTCCTGC GTTGGAATGG GCCGTCTCCC

651 ACAGGCAGCG CCTGCTGGAA CTCAACAGCT CCCTGGAGTT CAAGCTGCAC

701 CGACTGCACT TCATCCGCCT CTTGGCAGGA GGCCCCGCGA AGCAGCTGGA

751 GGCCCTCAGC TATGCTCGGC ACTTCCAGCC CTTTGCTCGG CTGCACCAGC

801 GGGAGATCCA GGTGATGATG GGCAGCCTGG TGTACCTGCG GCTGGGCTTG

851 GAGAAGTCAC CCTACTGCCA CCTGCTGGAC AGCAGCCACT GGGCAGAGAT

901 CTGTGAGACC TTTACCCGGG ACGCCTGTTC CCTGCTGGGG CTTTCTGTGG

951 AGTCCCCCCT TAGCGTCAGC TTTGCCTCTG GCTGTGTGGC GCTGCCTGTG 1001 TTGATGAACA TCAAGGCTGT GATTGAGCAG CGGCAGTGCA CTGGGGTCTG 1051 GAATCACAAG GACGAGTTAC CGATTGAGAT TGAACTAGGC ATGAAGTGCT 1101 GGTACCACTC CGTGTTCGCT TGCCCCATCC TCCGCCAGCA GACGTCAGAT 1151 TCCAACCCTC CCATCAAGCT CATCTGTGGC CATGTTATCT CCCGAGATGC 1201 ACTCAATAAG CTCATTAATG GAGGAAAGCT GAAGTGTCCC TACTGTCCCA 1251 TGGAGCAGAA CCCGGCAGAT GGGAAACGCA TCATATTCTG ATTCCTACCT 1301 GGAAGGAATT TTGTTGAAAG GGGTTTTCAC CTGTGAGCCT TGGTCTGTCT 1351 CGGTAGGGTG GTCAACTTCA GTGGACTGTG GTTGGTTTCA GAGCGCCTGG 1401 CTGAGGAGTT CCACTGAGGG GAGCACTGGA GCAGCCCTTT GGCAGAGGCT 1451 GAGGAGGGAG ATGGACCAGC CCACGCCTGG CACCTGGCTC CATGGCATAA 1501 GGAAAGGGAG ATGCTGGCCT CTGTGCTCCT GCTGTCTTTT CCTGTTTCTG 1551 TTTGCGTTTG ACTTAGTAGC AACCGACAGA GTGGCAAGGG ATTTGGTCTT 1601 CAGCAGTAGA CATCCTTCCA CCCCTGCCCT CAGCCAAGTC TCTTGCTGCC 1651 ATGCCAATGC TATGTCCACC CTTGCCCCTC GGCCCAAGAG TGTCCAGCGG 1701 TGGCCCACCT CTTCCTCCCA CTACAGCCTC AACAGTATGT ACCATCTCCC 1751 ACTGTAAATA GTCCCAGTTA GAACGGAATG CCGTTGTTTT ATAACTTTGA 1801 ACAAATGTAA AAAAAAAAA

BLAST Results

Entry HS579359 from database EMBL: human STS WI-6350. Score = 1027, P = 9.9e-40, identities = 207/209 Medline entries

No Medline entry

Peptide information for frame 2

ORF from 110 bp to 1288 bp; peptide length: 393 Category: similarity to unknown protein

1 MEQCACVERE LDKVLQKFLT YGQHCERSLE ELLHYVGQLR AELASAALQG 51 TPLSATLSLV MSQCCRKIKD TVQKLASDHK DIHSSVSRVG KAIDRNFDSE 101 ICGVVSDAVW DAREQQQQIL QMAIVEHLYQ QGMLSVAEEL CQESTLNVDL 151 DFKQPFLELN RILEALHEQD LGPALEWAVS HRQRLLELNS ΞLEFKLHRLH 201 FIRLLAGGPA KQLEALSYAR HFQPFARLHQ REIQVMMGSL VYLRLGLEKS 251 PYCHLLDSSH WAEICETFTR DACSLLGLSV ESPLSVSFAS GCVALPVLMN 301 IKAVIEQRQC TGVWNHKDEL PIEIELGMKC WYHSVFACPI LRQQTSDSNP 351 PIKLICGHVI SRDALNKLIN GGKLKCPYCP MEQNPADGKR IIF

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphmcfl_lall , frame 2

TREMBL: SPBC29A3_3 gene: "SPBC29A3.03c"; product: "hypothetical protein"; S. pombe chromosome II cosmid c29A3., N = 2, Score = 302, P = 3.4e-42

PIR:S67312 probable membrane protein YDR255c - yeast (Saccharomyces cerevisiae), N = 1, Score = 271, P = 5.3e-22

TREMBL :CET07D1_2 gene: "T07D1.2"; Caenorhabditis elegans cosmid T07D1., N = 1, Score = 193, P = 5.6e-13

>TREMBL:SPBC29A3_3 gene: "SPBC29A3.03c"; product: "hypothetical protein"; S. pombe chromosome II cosmid c29A3. Length = 398

HSPs:

Score = 302 (45.3 bits), Expect = 3.4e-42, Sum P(2) = 3.4e-42 Identities = 55/142 (38%), Positives = 89/142 (62%)

Query: 252 YCHLLDSSHWAEICETFTRDACSLLGLSVESPLSVSFASGCVALPVLMNIKAVIEQRQCT 311

Y +LD W + F R+ C+ LG+S+ESPL + +G +ALP+L+ + ++++++ Sbjct: 258 YIDVLDLD-WKSLELLFVREFCAALGMSLESPLDIVVNAGAIALPILLKMSSIMKKKHTE 316

Query: 312 GVWNHKDELPIEIELGMKCWYHSVFACPILRQQTSDSNPPIKLICGHVISRDALNKLING 371

W + ELP+EI L +HSVF CP+ ++Q ++ NPP+ + CGHVI +++L +L Sbjct: 317 —WTSQGELPVEIFLPSSYHFHSVFTCPVSKEQATEENPPMMMSCGHVIVKESLRQLΞRN 374

Query: 372 G—KLKCPYCPMEQNPADGKRIIF 393

G + KCPYCP E AD R+ F Sbjct: 375 GSQRFKCPYCPNENVAADAIRVYF 398

Score = 161 (24.2 bits), Expect = 3.4e-42, Sum P(2) = 3.4e-42 Identities = 51/221 (23%), Positives = 102/221 (46%)

Query: 22 GQHCERSLEELLHYVGQLRAELASAALQGTPLSATLSLVMSQCCRKIKDTVQKLASDHKD 81

G C L EL + + + L+ P ++ LV C K + L K Sbjct: 15 GNKCLAKLNEL ESILKDAKKSCLKD-PTTSMKELVA—CSEKTQQVFDDLKRTEKK 67

Query: 82 IHSSVSRVGKAIDRNFDΞEICGVVSDAVWDAREQQQQILQMAIVEHLYQQGMLSVAEELC 141

H+S++R GK +++ F+ ++ + + +++++++ + A+ H ++QG + +A C Sbjct: 68 FHTSLNRFGKTLEKKFNFDLEDIKLHSSFESKKRE IDTALSLHFFRQGDVELAHLFC 124

Query: 142 QESTLNVDLDFKQPFLELNRILEALHEQDLGPALEWAVSHRQRLLELNSSLEFKLHRLHF 201

+E+ + + F L I++ + ++DL +EWA R L SSLE+ L + Sbjct: 125 KEAGIEEPSESLHVFTLLKSIVQGIRDKDLKLPIEWASQCRGYLERKGSSLEYTLQKYRL 184

Query: 202 IRLLAGGPAKQL-EALSYAR-HFQPFARLHQREIQVMMGSLVY 242 + K + A+ Y R + F + H +IQ M +L + Sbjct: 185 VSNYL—TTKDIMAAIRYCRTNMAEFQKKHLADIQKTMIALFF 225

Pedant information for DKFZphmcfl_lall, frame 2

Report for DKFZphmcfl_lall .2

LENGTH] 393 MW] 44414.77 pi] 6.15 HOMOL] TREMBL :SPBC29A3 3 gene: "SPBC29A3.03c"; product: "hypothetical protein" pombe chromosome II cosmid c29A3. 2e-39

FUNCAT] 99 unclassified proteins [S. cerevisiae, YDR255c] 8e-23

PIRKW] transmembrane protein 2e-21

PROSITE] MYRISTYL 2

PROSITE] AMIDATION 1

PROSITE] CK2_PHOSPHO_SITE 3

PROSITE] PROKAR_LIPOPROTEIN 1

PROSITE] TYR_PHOSPHO_SITE 3

PROSITE] PKC_PHOSPHO_SITE 1

PROSITE] ASN_GLYCOSYLATION 1

KW] TRANSMEMBRANE 1

SEQ MEQCACVERELDKVLQKFLTYGQHCERSLEELLHYVGQLRAELASAALQGTPLSATLSLV PRD ccceeehhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccchhhhh

MEM

SEQ MSQCCRKIKDTVQKLASDHKDIHSSVSRVGKAIDRNFDSEICGVVSDAVWDAREQQQQIL PRD hhhhhhhhhhhhhhhhhhhhhhcccccccchhhhhccccceeeechhhhhhhhhhhhhhh MEM

SEQ QMAIVEHLYQQGMLSVAEELCQESTLNVDLDFKQPFLELNRILEALHEQDLGPALEWAVS PRD hhhhhhhhhhhccchhhhhhhhhhhccccccccchhhhhhhhhhhhhhccccchhhhhhh MEM

SEQ HRQRLLELNSSLEFKLHRLHFIRLLAGGPAKQLEALSYARHFQPFARLHQREIQVMMGSL PRD hhhhhhhcccchhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

MEM

SEQ VYLRLGLEKSPYCHLLDSSHWAEICETFTRDACSLLGLSVESPLSVSFASGCVALPVLMN PRD hhcccccccccccccccchhhhhhhhhhhhhhhhhhhhcccccceeeecccccchhhhhh

MEM MMMMMMMMMMMMMMMMMMMMMMM

SEQ IKAVIEQRQCTGVWNHKDELPIEIELGMKCWYHSVFACPILRQQTSDSNPPIKLICGHVI PRD hhhhhhhhhhhcccccccccceeeeeccceeeeeeeecchhhhhccccccccccccceee MEM MMMMMM

SEQ SRDALNKLINGGKLKCPYCPMEQNPADGKRIIF PRD eehhhhhhhccccccccccccccchhhhhcccc MEM

Prosite for DKFZphmcfl_lall .2

PS00001 189->193 ASN_GLYCOSYLATION PDOC00001

PΞ00005 180->183 PKC_PHOSPHO_SITE PDOC00005

PS00006 28->32 CK2_PHOSPHO_SITE PDOC00006

PS00006 135->139 CK2_PHOSPHO_SITE PDOC00006

PS00006 190->194 CK2_PHOSPHO_SITE PDOC00006

PS00007 211->219 TYR_PHOSPHO_SITE PDOC00007

PS00007 27->36 TYR_PHOSPHO_SITE PDOC00007

PS00007 244->253 TYR_PHOSPHO_SITE PDOC00007

PS00008 37->43 MYRISTYL PDOC00008

PS00008 50->56 MYRISTYL PDOC00008

PS00009 387->391 AMIDATION PDOC00009

PS00013 282->293 PROKAR LIPOPROTEIN PDOC00013

(No Pfam data available for DKFZphmcfl_lall .2) DKFZphmcfl_lc23

group: mammary carcinoma derived

DKFZphmcfl_lc23.1 encodes a novel 311 amino acid proline rich protein.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application m studying the expression profile of mamma carcinoma- specific genes. unknown, proline rich protein complete cDNA, complete eds? potential start at Bp 50, EST hits

Sequenced by DKFZ

Locus: unknown

Insert length: 3077 bp

Poly A stretch at pos. 3067, polyadenylation signal at pos. 3048

1 AACTGGCCCC CTCCCCCACC CCCTGCCCCT GAGGAGCAGG ACCTGTCCAT

51 GGCTGACTTC CCCCCACCAG AGGAGGCTTT TTTCTCTGTG GCCAGCCCTG

101 AGCCTGCAGG CCCTTCAGGC TCCCCAGAGC TTGTCAGCTC CCCGGCTGCT

151 TCGTCCTCCT CAGCTACTGC TTTGCAGATT CAGCCCCCGG GTAGCCCAGA

201 CCCTCCTCCA GCTCCGCCAG CCCCAGCTCC TGCTAGTTCC GCCCCAGGGC

251 ATGTGGCCAA GCTCCCTCAG AAGGAACCGG TGGGCTGTAG CAAGGGTGGT

301 GGGCCTCCCA GGGAGGACGT AGGTGCGCCC CTGGTCACGC CCTCGCTCCT

351 GCAGATGGTG CGGCTGCGCT CCGTGGGTGC TCCAGGAGGG GCTCCCACCC

401 CAGCACTGGG GCCATCGGCC CCCCAGAAAC CACTGCGAAG GGCCCTGTCA

451 GGGCGGGCCA GCCCAGTGCC TGCCCCCTCC TCAGGGCTCC ATGCTGCGGT

501 CCGACTCAAG GCCTGCAGCC TGGCCGCCAG TGAAGGCCTC TCAAGTGCTC

551 AGCCCAACGG ACCGCCTGAG GCAGAGCCAC GGCCTCCCCA GTCCCCTGCC

601 TCAACGGCCA GTTTCATCTT CTCCAAGGGC TCTAGGAAGC TGCAGCTGGA

651 GCGGCCCGTG TCCCCTGAGA CCCAGGCTGA CCTCCAGCGG AATCTGGTGG

701 CAGAACTCCG GAGCATCTCA GAGCAGCGGC CACCCCAGGC CCCAAAGAAG

751 TCACCTAAGG CTCCCCCACC TGTGGCCCGC AAGCCGTCTG TGGGAGTCCC

801 CCCACCCGCC TCCCCCAGTT ACCCTCGAGC TGAGCCCCTT ACTGCTCCTC

851 CCACCAATGG GCTCCCTCAC ACCCAGGACA GGACTAAGAG GGAGCTGGCG

901 GAGAATGGAG GTGTCCTGCA GCTGGTGGGC CCAGAGGAGA AGATGGGCCT

951 CCCGGGCTCA GACTCACAGA AAGAGCTGGC CTGACCACCA GGCACCTCAC

1001 TGGCACTGCT GACCCATCCC AGAAACACAA TCTCAGGGAC CCGAGCAGCT

1051 CCAAGGACGA GAGGATACAG CAGACACAAC CTAATAGAGA GGGCGCCTGC

1101 AGCCTTAACC TCCACGGCCT TCGATACTTA TGCAAGCCTG GTGTTGCTCC

1151 TGTCCTCAGA GTCATCCTGC GCTCATGCCT TTTCCCGAAT GGGTTCACCT

1201 CTGGCAGTTG CCGCTTCAGT CTTGGCCTTA GCCTCATCTT GAAGTGGGTA

1251 GCTGGCGGGA GAGGGTGGCT GCGCCCCCTG CTGGCCCTGA GGCTGCAGAG

1301 TTGGGAGCAG GACACCTCAC CTGAGTTTCA TTTTTTTTCA TGTCCAAACC

1351 ATGCACATAC TATAGTCCAG AATCAAAGCA CTTTTGAAAA GTGGCTGCAT

1401 GGCCATCCTC CAGGGCCCAG GAAGTTGCAT TCCAAGGGCC TGTTTACATG

1451 GCAGCAGAAT CCATCCCCGG CAGTCAGCCC ATAGCTTGGG ACCAGTCTGT

1501 GCCCTCCTGC CCAGTCCAGT TTACTCCTCT TGGTTCCTGA AGGTGGCCAA

1551 GTCATTGTGT TCCCACAGGC TTCTCTAGGC TGGGGGCAGG TGTGGGGCTG

1601 TGGAATTCCA AAGCACAAAA GGTGCAGAGG GGATTGGCCT TCCTGTGCCT

1651 CAACTCACCA ACCACCCTCC TGCCTTCCAG TTCTGCCAGG TGCTCCATGC

1701 TGGGGACAAG TAGGAGACTG CCAGGGCCCA AAGAAATGGG TGAGCAGTAG

1751 AGTCATCTCG GGGCACTTGG CAGTGTCAAG CACCTGCCCC TTGCCTCCTT

1801 GACCACACTG GGGTGGGTGG GCCCCCAGCA CTTCAGAGGC AGGAGCCTTT

1851 GGGCTGAGCA AGCACTGAGG AGGTGGATGG AAGGGAGCAT CTGGAGGGGG

1901 GGAGCTTCCT TGAGCAGTGG GCCCAGGCCT GGCCCTCCAC ACTTCATTCT

1951 CTGACCTTTC TCTCTCCTCA TTTCGGTGCA TGTCCTTTCT GCAGCTGCCT

2001 TTCAGCACAG GTGGTTCCAC TGGGGGCAGC TAACGCTGAG TGACAAGGAT

2051 GGGAAGCCAC AGGTGCATTT TACTCAAGTC TTCTCTAGTC AATGAGGGGC

2101 ACCCAGTGCT TCTAGGGCAG GCTGGGTGGT GGTCCCCTAG GTATCAGCCT

2151 CTCTTACTGT ACTCTCCGGG AATGTTAACC TTTCTATTTT CAGCCTGTGC

2201 CACCTGTCTA GGCAAGCTGG CTTCCCCATT GGCCCCTGTG GGTCCACAGC

2251 AGCGTGGCTG CCCCCCAGGG CCACCGCTTC TTTCTTGATC CTCTTTCCTT

2301 AACAGTGACT TGGGCTTGAG TCTGGCAAGG AACCTTGCTT TTAGCTTCAC

2351 CACCAAGGAG AGAGGTTGAC ATGACCTCCC CGCCCCCTCA CCAAGGCTGG

2401 GAACAGAGGG GATGTGGTGA GAGCCAGGTT CCTCTGGCCC TCTCCAGGGT

2451 GTTTTCCACT AGTCACTACT GTCTTCTCCT TGTAGCTAAT CAATCAATAT

2501 TCTTCCCTTG CCTGTGGGCA GTGGAGAGTG CTGCTGGGTG TACGCTGCAC

2551 CTGCCCACTG AGTTGGGGAA AGAGGATAAT CAGTGAGCAC TGTTCTGCTC

2601 AGAGCTCCTG ATCTACCCCA CCCCCTAGGA TCCAGGACTG GGTCAAAGCT

2651 GCATGAAACC AGGCCCTGGC AGCAACCTGG GAATGGCTGG AGGTGGGAGA

2701 GAACCTGACT TCTCTTTCCC TCTCCCTCCT CCAACATTAC TGGAACTCTA 2751 TCCTGTTAGG ATCTTCTGAG CTTGTTTCCC TGCTGGGTGG GACAGAGGAC 2801 AAAGGAGAAG GGAGGGTCTA GAAGAGGCAG CCCTTCTTTG TCCTCTGGGG 2851 TAAATGAGCT TGACCTAGAG TAAATGGAGA GACCAAAAGC CTCTGATTTT 2901 TAATTTCCAT AAAATGTTAG AAGTATATAT ATACATATAT ATATTTCTTT 2951 AAATTTTTGA GTCTTTGATA TGTCTAAAAA TCCATTCCCT CTGCCCTGAA 3001 GCCTGAGTGA GACACATGAA GAAAACTGTG TTTCATTTAA AGATGTTAAT 3051 TAAATGATTG AAACTTGAAA AAAAAAA

BLAST Results

No BLAST result

Medline entries

No Medlme entry

Peptide information for frame 1

ORF from 49 bp to 981 bp; peptide length: 311 Category: putative protein Classification: unset

1 MADFPPPEEA FFSVASPEPA GPSGSPELVS SPAASSSSAT ALQIQPPGSP

51 DPPPAPPAPA PASSAPGHVA KLPQKEPVGC SKGGGPPRED VGAPLVTPSL

101 LQMVRLRSVG APGGAPTPAL GPSAPQKPLR RALSGRASPV PAPSSGLHAA

151 VRLKACSLAA SEGLSSAQPN GPPEAEPRPP QSPASTASFI FSKGSRKLQL

201 ERPVSPETQA DLQRNLVAEL RSISEQRPPQ APKKSPKAPP PVARKPSVGV

251 PPPASPSYPR AEPLTAPPTN GLPHTQDRTK RELAENGGVL QLVGPEEKMG

301 LPGSDSQKEL A

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphmcfl_lc23, frame 1

PIR:S49915 extensm-like protein - maize, N = 1, Score = 215, P = 6.1e-15

PIR:A28996 prolme-rich protein M14 precursor - mouse, N = 1, Score = 191, P = 3.8e-13

>PIR:S49915 extensin-like protein - maize

Length = 1, 188

HSPs:

Score = 215 (32.3 bits), Expect = 6.1e-15, P = 6.1e-15 Identities = 81/269 (30%), Positives = 115/269 (42%)

Query: 5 PPPEEAFFS VASPEPAGPSGSPELVSSPAASSSSATALQIQPPGSP—DPPP A 55

PPP S V SP P P SP PA +SS ++ PP +P PPP + Sbjct: 598 PPPPAPVASPPPPVKSPPPPTPVASPP PPAPVASSPPPMKSPPPPTPVSSPPPPEKS 654

Query: 56 PPAPAPASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGA 115

PP P PA S P + P P K PP + + P + PS + P Sbjct: 655 PPPPPPAKSTPPP-EEYPT—PPTSVKSSPPPEKSLPPPTLIPSPPPQEKPTPPSTPSKP 711

Query: 116 PTPALGPSAPQKPLRRA-LSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPE 174

P+ PS P++P+ + ++SP PAP S +LA S + + PP

Sbjct: 712 PSSPEKPSPPKEPVSSPPQTPKSSPPPAPVSSPPPTPVSSPPALAPVSSPPSVKSSPPPA 771

Query: 175 AEPRPPQSPAΞTASFIFSKGSRKLQLERPV-SPETQADLQRNLVAELRSISEQRPPQAPK 233

PP +P +S +Q+ P +P++ L V+ + + PP AP

Sbjct: 772 PLSSPPPAPQVKΞS PPPVQVSSPPPAPKSSPPLAP—VSSPPQVEKTSPPPAPL 823

Query: 234 KSPKAPPPVARKPSVGV—PPPASPSYPRAEPLTAPPTNGLP 273

SP p + p v v ppp s p P+++PP P

Sbjct: 824 SSPPLAPK-SSPPHVVVSSPPPVVKSSPPPAPVSSPPLTPKP 864 Score = 206 (30.9 bits), Expect = 9. le-14, P = 9. le-14 Identities = 82/261 (31%), Positives = 108/261 (41%)

Query: 17 PEPAG-PSGSPELVΞΞPAASS SSATALQIQPPGSPDPPPAP PAPAPASSAPGHV 69

P P G P SP + PAAS+ S T + P P+P P P P P P +P Sbjct: 410 PTPGGGPPSSP-VPGKPAASAPMPSPHTPPDVSPEPLPEPSPVPAPAPMPMPTPHSPPAD 468

Query: 70 AKLPQKEPV-GCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTPALGPSAPQKP 128

+P PV G S P V P + +V+L AP G+P P + ++P P Sbjct: 469 DYVPPTPPVPGKΞPPATSPSPQVQPPAASTPPPSLVKLSPPQAPVGSPPPPVKTTSPPAP 528

Query: 129 LRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEAEPRPPQSPASTAS 188

+ G SP P P S + +K+ A G + P PPE P PP AS Sbjct: 529 I GSPSP-PPPVSVVSPPPPVKSPPPPAPVG SPP—PPEKSPPPPAPVASPPP 577

Query: 189 FIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQAPKKSPKAPPPVARKPS- 247

+ S L P P ++ VA + PP P SP P PVA P Sbjct: 578 PVKSPPPPTLVASPP—PPVKSPPPPAPVASPPPPVKSPPPPTPVASPPPPAPVASSPPP 635

Query: 248 VGVPPP ASPSYPRAEPLTAPPTNGLPHTQD 277

+ PPP +SP P P PP P ++ Sbjct: 636 MKSPPPPTPVSSPPPPEKSPPPPPPAKSTPPPEE 669

Score = 202 (30.3 bits), Expect = 2.9e-13, P = 2.9e-13 Identities = 81/254 (31%), Positives = 110/254 (43%)

Query: 16 SPEPAGPSGSPELV—SSP—AASSSSATALQIQPPGSP-DPPPAPPAPAPASSAPGHVA 70

SP PA P SP L SSP SS ++ PP +P PP P PA S P HV+ Sbjct: 817 SPPPA-PLSSPPLAPKSSPPHVVVSSPPPVVKSSPPPAPVSSPPLTPKPA SPPAHVS 872

Query: 71 KLPQ KEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTPALGPSAPQ 126

P+ P + PP E +P TP L ++S P +P + P + Sbjct: 873 SPPEVVKPSTPPAPTTVISPPSEPKSSPPPTPVSLPPPIVKSSPPPAMVSΞPPMTPKSSP 932

Query: 127 KPLRRAL SGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEAEPRPPQSP 183

P+ + + ++SP PAP S A K+ A L P PPE + PP +P Sbjct: 933 PPVVVSSPPPTVKSSPPPAPVSSPPATP—KSSPPPAPVNL P—PPEVKSSPPPTP 984

Query: 184 ASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQAPKKSPKAPPPVA 243

S+ + P PE ++ V+ + PP AP SP PPPV

Sbjct: 985 VSSPPPAPKSSPPPAPMSΞPPPPEVKSPPPPAPVSSPPPPVKSPPPPAPVSSP—PPPVK 1042

Query: 244 RKPS VGVPPPASPΞYPRAEPLTAPP 268

P V PPP S P P+++PP Sbjct: 1043 SPPPPAPVSSPPPPVKSPPPPAPISSPP 1070

Score = 190 (28.5 bits), Expect = 7.9e-12, P = 7.9e-12 Identities = 74/264 (28%), Positives = 111/264 (42%)

Query: 5 PPPEEAFFSVASPEPAGPSGSPELVSSPAAS-SSSATALQIQPPGSPDPPPAPPAPAPAS 63

PPP S PE + P P + P + T+++ PP PP P+P Sbjct: 639 PPPPTPVSSPPPPEKSPPPPPPAKSTPPPEEYPTPPTSVKSSPPPEKSLPPPTLIPSPPP 698

Query: 64 SAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTPALGPS 123

P K P K PP+E V +P TP V +P PTP P

Sbjct: 699 QEKPTPPSTPSKPPSSPEKPS-PPKEPVSSPPQTPK—SSPPPAPVSSP—PPTPVSSPP 753

Query: 124 APQKPLRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEAEPRPPQSP 183

A P+ S ++SP PAP S A ++K+ + + + P PP + PP +P Sbjct: 754 A-LAPVSSPPSVKSSPPPAPLSSPPPAPQVKS SPPPVQVSSP—PPAPKΞSPPLAP 806

Query: 184 ASTASFIFSKGSRKLQLERP-VSPETQADLQRNLVAELRSISEQRPPQAPKKSPKAPPPV 242

S+ + L P ++P++ +V+ + + PP AP SP P

Sbjct: 807 VSSPPQVEKTSPPPAPLSSPPLAPKSSPP—HVVVSSPPPVVKSSPPPAPVSSPPLTPKP 864

Query: 243 ARKPS-VGVPP PASPSYPR AEPLTAPP 268

A P+ V PP P++P P +EP ++PP Sbjct: 865 ASPPAHVSSPPEVVKPSTPPAPTTVISPPSEPKSSPP 901

Score = 189 (28.4 bits). Expect = l.Oe-ll, P = l.Oe-11 Identities = 86/271 (31%), Positives = 112/271 (41%)

Query: 5 PPPEEAFFSVASPEPAGPSGSPEL-VSSP—AASSSSATALQIQPPG—SPDPPPAP 56

PPP A S P P S P + VSSP A SS A PP PPPAP Sbjct: 768 PPP--APLSSPPPAPQVKSSPPPVQVSSPPPAPKSSPPLAPVSSPPQVEKTSPPPAPLSS 825

Query: 57 PAPAPASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAP 116

P AP SS P V P PV S PP V +P +TP V +P

Sbjct: 826 PPLAPKSSPPHVVVSSPP—PVVKSS PPPAPVSSPPLTPKPASPPA—HVSSPPEVV 878

Query: 117 TPALGPSAPQKPLRRALSGRASPVPAPSSGLHAAVRLKAC-SLAASEGL SSAQP 169

P+ P AP + ++SP P P S V+ ++ +S + SS P Sb_jct: 879 KPST-PPAPTTVISPPSEPKSSPPPTPVSLPPPIVKSSPPPAMVSSPPMTPKSSPPPWV 937

Query: 170 -NGPPEAEPRPPQSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRP 228

+ PP + PP +P S+ + P PE ++ V+ + P

Sb_jct: 938 SSPPPTVKSSPPPAPVSSPPATPKSSPPPAPVNLP-PPEVKSSPPPTPVSSPPPAPKSSP 996

Query: 229 PQAPKKΞPKAPPPVARKPS VGVPPPASPSYPRAEPLTAPP 268

P AP SP PPP + P V PPP S P P+++PP Sbjct: 997 PPAPMSSP—PPPEVKSPPPPAPVSΞPPPPVKSPPPPAPVSSPP 1038

Score = 181 (27.2 bits), Expect = 8.8e-ll, P = 8.8e-ll Identities = 73/277 (26%), Positives = 105/277 (37%)

Query: 3 DFPPPEEAFFSVASPEPAGPSGSPELVSSPAASSSSATALQIQPP GSPDPP PA 55

D+ PP V P S SP+ V PAAΞ+ + +++ PP GSP PP + Sbjct: 469 DYVPPTPP VPGKSPPATSPSPQ-VQPPAASTPPPSLVKLSPPQAPVGSPPPPVKTTS 524

Query: 56 PPAPAPASSAPGHVAKL PQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGA 111

PPAP + S P V+ + P K P + G PP + P P ++S Sbjct: 525 PPAPIGSPSPPPPVSVVΞPPPPVKSPPPPAPVGSPPPPEKSPPPPAPVASPPPPVKSPPP 584

Query: 112 PG—GAPTPALGPSAPQKPLRRA LSGRASPVPAPSSGLHAAVRLKACSLAASEGLSS 166

P +P P + P P+ + P P S A V + + + Sbjct: 585 PTLVASPPPPVKSPPPPAPVASPPPPVKSPPPPTPVASPPPPAPVASSPPPMKSPPPPTP 644

Query: 167 AQPNGPPEAEPRPPQSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQ 226

PPE P PP PA + + ++ PE L+ +

Sbjct: 645 VSSPPPPEKSP-PPPPPAKSTPPPEEYPTPPTSVKSSPPPEKSLP-PPTLIPSPPPQEKP 702

Query: 227 RPPQAPKKSPKAPP-PVARKPSVGVPPPASPSYPRAEPLTAPP 268

PP P K P +P P K V PP S P P+++PP Sbjct: 703 TPPSTPSKPPSSPEKPSPPKEPVSSPPQTPKSΞPPPAPVSSPP 745

Score = 177 (26.6 bits), Expect = 2.6e-10, P = 2.6e-10 Identities = 78/₂64 (29%), Positives = 105/264 (39%)

Query: 5 PPPEEAFFSVASPEPAGP SGSPELVSSPAASSSSATALQIQPPGSP—DPPPAP— 56

PPP +P+PA P S PE+V P+ + T I PP P PPP P Sbjct: 850 PPPAPVSSPPLTPKPASPPAHVSSPPEVVK-PSTPPAPTTV—ISPPSEPKSSPPPTPVS 906

Query: 57 -PAPAPASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGA 115

P P SS P + P P PP V +P P++ V +P Sbjct: 907 LPPPIVKSSPPPAMVSSPPMTPKS SPPPVVVSSP—PPTVKSSPPPAPVSSPPAT 959

Query: 116 PTPALGPSAPQKPLRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEA 175

P + P+ P ++SP P P S A + S +SS P PPE Sbjct: 960 PKSSPPPAPVNLPPPEV KSSPPPTPVSSPPPAPK SSPPPAPMSSP-P—PPEV 1009

Query: 176 EPRPPQSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQAPKKS 235

+ PP +P S+ + P P ++ V+ + PP AP S

Sbjct: 1010 KSPPPPAPVSSPPPPVKSPPPPAPVSSP-PPPVKSPPPPAPVSSPPPPVKSPPPPAPISS 1068

Query: 236 PKAPPPVARKPS VGVPPPASPSYPRAEPLTAPP 268

P PPPV P V PPP S P P+++PP Sbjct: 1069 P—PPPVKSPPPPAPVSSPPPPVKSPPPPAPVSSPP 1102

Score = 177 (26.6 bits), Expect = 2.6e-10, P = 2.6e-10 Identities = 82/267 (30%), Positives = 110/267 (41%)

Query: 17 PEPAG-PSGSPELVSSPAASS SSATALQIQPPGSPDPPPAP PAPAPASSAPGHV 69

P P G P SP + PAAS+ S T + P P+P P P P P P +P Sbjct: 410 PTPGGGPPSSP-VPGKPAASAPMPSPHTPPDVSPEPLPEPSPVPAPAPMPMPTPHSPPAD 468

Query: 70 AKLPQKEPV-GCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTPALGPSAPQKP 128

+P PV G S P V P + +V+L AP G+P P + ++P P Sbjct: 469 DYVPPTPPVPGKSPPATSPSPQVQPPAASTPPPSLVKLSPPQAPVGSPPPPVKTTSPPAP 528

Query: 129 LRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEAEPRPPQSPASTAS 188

+ G SP P P S + +K+ A G + P PPE P PP AS Sbjct: 529 I GSPSP-PPPVSVVSPPPPVKSPPPPAPVG SPP—PPEKSPPPPAPVASPPP 577

Query: 189 FIFSKGSRKLQLERPV SPETQADLQRNLVAELRS ISEQRPPQA PK 233

+ S L P SP A + + ++S ++ PP P Sbjct: 578 PVKSPPPPTLVASPPPPVKSPPPPAPVA-SPPPPVKSPPPPTPVASPPPPAPVASSPPPM 636

Query: 234 KSPKAPPPVARKP SVGVPPPASPSYPRAEPLTAPPTN 270

KSP P PV+ P PPP + S P E PPT+ Sbjct: 637 KSPPPPTPVSSPPPPEKSPPPPPPAKSTPPPEEYPTPPTS 676

Score = 170 (25.5 bits), Expect = 1.6e-09, P = 1.6e-09 Identities = 78/279 (27%), Positives = 108/279 (38%) Query: 5 PPPEEAFFSVASPEPAGPSGSPELVSSPAASSSSATALQIQPPGSPDPPPAPPAPAPASS 64

PP S S + P +P + P SS A+ PP +P +PP P SS Sbjct: 883 PPAPTTVISPPSEPKSSPPPTPVSLPPPIVKSSPPPAMVSSPPMTPKS—SPP-PVVVSS 939

Query: 65 APGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPG—GAPTPALGP 122

P V P PV PP +P P L ++S P +P PA Sbjct: 940 PPPTVKSSPPPAPVS SPPATPKSSPPPAPVNLPPPEVKSSPPPTPVSSPPPAPKS 994

Query: 123 SAPQKPLRRALSG--RASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEAEPRPP 180

S P P+ ++ P PAP S V+ S +SS P PP + PP Sbjct: 995 SPPPAPMSSPPPPEVKSPPPPAPVSSPPPPVK SPPPPAPVSS--P--PPPVKSPPP 1046

Query: 181 QSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQAPKKSPKAPP 240

+P S+ + P P ++ V+ + PP AP SP PP

Sbjct: 1047 PAPVΞSPPPPVKSPPPPAPISSP-PPPVKSPPPPAPVSSPPPPVKSPPPPAPVSSP—PP 1103

Query: 241 PVARKPS VGVPPPAS PSYPRAEPLTAPPTNGLPHTQDRTKREL 283

P+ P V PPPA PS P P+++PP P + ++ L Sbjct: 1104 PIKSPPPPAPVΞSPPPAPVKPPSLPPPAPVSSPPPVVTPAPPKKEEQSL 1152

Score = 169 (25.4 bits). Expect = 2. le-09, P = 2. le-09 Identities = 75/266 (28%), Positives = 104/266 (39%)

Query: 3 DFPPPEEAFFSVASPEPAGPSGSPELVSSPAASSSSATALQIQPP GSPDPP PA 55

D+ PP V P S SP+ V PAAS+ + +++ PP GSP PP + Sbjct: 469 DYVPPTPP VPGKSPPATSPSPQ-VQPPAASTPPPSLVKLSPPQAPVGSPPPPVKTTS 524

Query: 56 PPAPAPASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGA 115

PPAP + S P V+ + PV PP VG+P P V +P Sbjct: 525 PPAPIGSPSPPPPVSVVSPPPPVKSP PPPAPVGSP—PPPEKSPPPPAPVASP 575

Query: 116 PTPALGPSAPQKPLRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEA 175

P P P P ++ P PAP + V+ S ++S P P + Sbjct: 576 PPPVKSPPPPTLVASPPPPVKSPPPPAPVASPPPPVK SPPPPTPVASPPPPAPVAS 631

Query: 176 EPRPPQSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQAPKKS 235

P P +SP K P P S+ PP+

Sbjct: 632 SPPPMKSPPPPTPVSSPPPPEKSP—PPPPPAKSTPPPEEYPTPPTSVKSSPPPEKSLPP 689

Query: 236 PK APPPVARK—PSVGVPPPASPSYPRA—EPLTAPP 268

P +PPP + PS PP+SP P EP+++PP Sbjct: 690 PTLIPSPPPQEKPTPPSTPSKPPΞSPEKPSPPKEPVSSPP 729

Score = 168 (25.2 bits), Expect = 2.7e-09, P = 2.7e-09 Identities = 75/267 (28%), Positives = 102/267 (38%)

Query: 2 ADFPPPEEAFFSVASPE-PAGPSGSPELVSSPAASSSSATALQIQPPGSPDPP-PAPPAP 59

A PPP + ++ P+ P G P +SP A S + SP PP +PP P Sbjct: 496 ASTPPP—SLVKLΞPPQAPVGSPPPPVKTTSPPAPIGSPSPPPPVΞVVSPPPPVKSPPPP 553

Query: 60 APASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTPA 119

AP S P P PV PP + P + S V+ AP +P P Sbjct: 554 APVGSPPPPEKSPPPPAPVASPP PPVKΞPPPPTLVASPPPPVKSPPPPAPVASPPPP 610

Query: 120 LGPSAPQKPLRRALSGRASPVPAPSSGLHAAVRLKACSL-AASEGLSSAQPNGPPEAEPR 178

+ P P+ + P PAP + ++ +S P PP A+ Sbjct: 611 VKSPPPPTPVA SPPPPAPVASSPPPMKSPPPPTPVSSPPPPEKSPPPPPPAKST 664

Query: 179 PP--QSPASTASFIFSKGSRKLQLERPV SPETQADLQRNLVAELRSISEQRPPQAPK 233

PP + P S S K L P SP Q S ++P +P

Sbjct: 665 PPPEEYPTPPTSVKSSPPPEK-SLPPPTLIPSPPPQEKPTPPSTPSKPPSSPEKP—SPP 721

Query: 234 KSPKAPPPVARKPSVGVPPPASPSYPRAEPLTAPP 268

K P + PP K S PPPA S P P+++PP Sbjct: 722 KEPVSSPPQTPKSS PPPAPVSSPPPTPVSSPP 753

Score = 166 (24.9 bits), Expect = 4.6e-09, P = 4.6e-09 Identities = 81/268 (30%), Positives = 108/268 (40%)

Query: 5 PPPEEAF FSVASPEPAGPSGSPE-LVSSPAAΞSSS ATALQIQPPGSPDPPP-- 54

PPPE++ VASP P Ξ P LV+SP S A PP PPP Sbjct: 560 PPPEKSPPPPAPVASPPPPVKSPPPPTLVASPPPPVKSPPPPAPVASPPPPVKSPPPPTP 619

Query: 55 --APPAPAPASSAPGHVAKLPQKEPVGC SKGGGPPREDVGAPLVTPSLLQMVRLRS 108

+PP PAP +S+P + P PV K PP P ++S

Sbjct: 620 VASPPPPAPVASSPPPMKSPPPPTPVSΞPPPPEKSPPPPPPAKSTPPPEEYPTPPTSVKS 679

Query: 109 VGAPGGA-PTPALGPSAPQKPLRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSA 167

P + P P L PS P P + + ++P PSS + + Ξ SS Sbjct: 680 SPPPEKSLPPPTLIPSPP—PQEKP-TPPSTPSKPPSSPEKPSPPKEPVSSPPQTPKSSP 736 Query: 168 QPNGPPEAEPRPPQSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQR 227

P P P SP + A + S Ξ K P +P + + + + Sbjct: 737 PPAPVSSPPPTPVΞSPPALAP-VSSPPSVKSS--PPPAPLSSPPPAPQVKSSPPPVQVSS 793

Query: 228 PPQAPKKSPKAPPPVARKPSVGVPPPASPSYPRAEPLTAPP 268

PP APK SP P+A P V PP + P PL++PP Sbjct: 794 PPPAPKSSP PLA--P-VSΞPPQVEKTSPPPAPLSSPP 827

Score = 165 (24.8 bits), Expect = 6.0e-09, P = 6.0e-09 Identities = 79/264 (29%), Positives = 105/264 (39%)

Query: 5 PPPEEAFFSVASPEPAG-PSGSP--ELVSSPAASSSSATALQIQPPGSPDPPP-APPAPA 60

PPP + + + P P G PS P +VS P S P GSP PP +PP PA Sbjct: 517 PPPVK TTSPPAPIGSPSPPPPVSVVSPPPPVKSPPPPA PVGSPPPPEKSPPPPA 570

Query: 61 PASSAPGHVAKLPQKEPVGCSKG GGPPREDVGAP LVTPSLLQMVRLRSVGAPGG 114

P +S P V P V PP V +P + +P V AP

Sbjct: 571 PVASPPPPVKSPPPPTLVASPPPPVKSPPPPAPVAΞPPPPVKSPPPPTPVASPPPPAPVA 630

Query: 115 APTPALGPSAPQKPLRRALSGRASPVPAP SSGLHAAVRLKACSLAASEGLSSAQPNG 171

+ P + P P+ SP P P S+ S+ +S + P

Sbjct: 631 ΞSPPPMKSPPPPTPVSSPPPPEKSPPPPPPAKSTPPPEEYPTPPTSVKSSPPPEKSLP— 688

Query: 172 PPEAEPRPPQSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQA 231

PP P PP T SK P SPE + + V+ + PP A

Sbjct: 689 PPTLIPSPPPQEKPTPPΞTPSKP PSSPEKPSP-PKEPVSSPPQTPKSSPPPA 739

Query: 232 PKKSPKAPPPVARKPSVGV—PPPASPSYPRAEPLTAPP 268

P SP P PV+ P++ PP+ S P PL++PP Sbjct: 740 PVSSPP-PTPVSSPPALAPVSSPPSVKSSPPPAPLSSPP 777

Score = 162 (24.3 bits), Expect = 1.3e-08, P = 1.3e-08 Identities = 76/27₂ (₂7%), Positives = 99/₂7₂ (36%)

Query: 2 ADFPPPEEAFFSVASPEPAG-PSGSPELVSSPAASSSSATALQIQPPGSPDPPPAPPAPA 60

A P P SPEP PS P P + S A PP P P +PPA + Sbjct: 427 ASAPMPSPHTPPDVSPEPLPEPSPVPAPAPMPMPTPHSPPADDYVPPTPPVPGKSPPATS 486

Query: 61 PASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTP-- 118

P+ A P V S PP+ VG+P P V+ S AP G+P+P Sbjct: 487 PSPQVQPPAASTPPPSLVKLS PPQAPVGSP—PPP VKTTSPPAPIGSPSPPP 536

Query: 119 ALGPSAPQK-PLRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPE 174

+ P P K P A G SP P S A S + + PP

Sbjct: 537 PVSVVSPPPPVKSPPPPAPVG—SPPPPEKSPPPPAPVASPPPPVKΞPPPPTLVASPPPP 594

Query: 175 AEPRPPQSPAΞTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQAPKK 234

+ PP +P ++ + P P A + + PP P+K

Sbjct: 595 VKSPPPPAPVASPPPPVKSPPPPTPVASPPPPAPVASSPPPMKSPPPPTPVSSPPP-PEK 653

Query: 235 SPKAPPPVARKPSVGVPPPASPSYPRAEPLTAPPTNGLP 273

SP PPP P PP P+ P + + PP LP Sbjct: 654 SPPPPPPAKSTP PPEEYPTPPTSVKSSPPPEKSLP 688

Score = 159 (23.9 bits), Expect = 2.8e-08, P = 2.8e-08 Identities = 77/264 (29%), Positives = 103/264 (39%)

Query: 5 PPPEEAFFSVASPEPAGPSGSPELVSSPAASSSSATALQIQPPGSP—DPPPAP PAP 59

PPP V+SP P P SP P SS ++ PP +P PP P P P Sbjct: 916 PPPA MVSSP-PMTPKSSPP PVVVSSPPPTVKSSPPPAPVSSPPATPKSΞPPP 966

Query: 60 APASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTPA 119

AP + P V P PV S P AP+ +P + V+ AP +P P Sbjct: 967 APVNLPPPEVKSSPPPTPVS-SPPPAPKSSPPPAPMSSPPPPE-VKSPPPPAPVSSPPPP 1024

Query: 120 LGPSAPQKPLRRALΞG-RASPVPAPSSGLHAAVRLKACSLAASEG LSSAQPNGPPEA 175

+ P P+ ++ P PAP S V+ S + S P P + Sbjct: 1025 VKSPPPPAPVSSPPPPVKSPPPPAPVSSPPPPVKSPPPPAPIΞSPPPPVKSPPPPAPVSS 1084

Query: 176 EPRPPQSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQAPKKS 235

P P +SP A S ++ P P A + A ++ S PP AP S Sbjct: 1085 PPPPVKSPPPPAPV SSPPPPIKSPPPP APVSSPPPAPVKPPS--LPPPAPVSS 1135

Query: 236 PK--APPPVARKPSVGVPPPA-SPSYPRAEPLTAPP 268

P P +K +PPPA S P + PP Sbjct: 1136 PPPVVTPAPPKKEEQSLPPPAESQPPPSFNDIILPP 1171

Score = 143 (21.5 bits), Expect = 1.8e-06, P = 1.8e-06 Identities = 59/179 (32%), Positives = 77/179 (43%) Query: 3 DFPPPEEAFFSVASPEP-AGPSGSPELVSSPAASSSSATA-LQIQPPGSP—DPPP A 55

+ PPPE Ξ P P + P +P+ PA SS ++ PP +P PPP + Sbjct: 970 NLPPPEVK—SSPPPTPVSSPPPAPKSSPPPAPMSSPPPPEVKSPPPPAPVSSPPPPVKS 1027

Query: 56 PPAPAPASSAPGHVAKLPQKEPVGCΞKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGA 115

PP PAP SS P V P PV PP + P Ξ V+ AP + Sbjct: 1028 PPPPAPVSSPPPPVKSPPPPAPVSSPP PPVKSPPPPAPISSPPPPVKSPPPPAPVSS 1084

Query: 116 PTPALGPSAPQKPLRRALSG-RASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPE 174

P P + P P+ ++ P PAP S A +K SL +SS P PP Sbjct: 1085 PPPPVKSPPPPAPVSSPPPPIKSPPPPAPVSSPPPAP-VKPPSLPPPAPVSS—P—PPV 1139

Query: 175 AEPRPPQ 181

P PP+ Sbjct: 1140 VTPAPPK 1146

Score = 133 (20.0 bits), Expect = 2.3e-05, P = 2.3e-05 Identities = 50/132 (37%), Positives = 59/132 (44%)

Query: 1 MADFPPPEEAFFSVASPEPAGP-SGSPELVSSP AASSSSATALQIQPPGSP—DPPP 54

M+ PPPE V SP P P S P V SP A SS ++ PP +P PPP Sbjct: 1001 MSSPPPPE VKSPPPPAPVSSPPPPVKSPPPPAPVSSPPPPVKSPPPPAPVSSPPP 1055

Query: 55 APPAPAPASSAPGHVAKLPQKEPVGCSKG GGPPREDVGAPLVTPSLLQMVRLRS 108

+PP PAP SS P V P PV PP V +P P +

Sbjct: 1056 PVKSPPPPAPISSPPPPVKSPPPPAPVSSPPPPVKSPPPPAPVSSP—PPPIKSPPPPAP 1113

Query: 109 VGAPGGAPT—PALGPSAP 125

V +P AP P+L P AP Sbjct: 1114 VSSPPPAPVKPPSLPPPAP 1132

Score = 110 (16.5 bits), Expect = 8.0e-03, P = 8.0e-03 Identities = 41/121 (33%), Positives = 49/121 (40%)

Query: 5 PPPEEAFFS VASPEPAGP-SGSPELVSSP AASSSSATALQIQPPGSP—DPPP 54

PPP S V SP P P S P V SP A SS ++ PP +P PPP Sbjct: 1060 PPPPAPISSPPPPVKSPPPPAPVSSPPPPVKΞPPPPAPVSSPPPPIKSPPPPAPVSSPPP 1119

Query: 55 AP PAPAPASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRS 108

AP P PAP SS P V P K+ + PP E P +L + Sbjct: 1120 APVKPPSLPPPAPVSSPPPVVTPAPPKKE EQSLPPPAESQPPPSFNDIILPPIMANK 1176

Query: 109 VGAP 112

+P Sbjct: 1177 YASP 1180

Score = 108 (16.2 bits), Expect = 1.3e-02, P = 1.3e-02 Identities = 46/155 (29%), Positives = 67/155 (43%)

Query: 114 GAPTPALGPSAPQKPLRRALSGRASPVPAPSSGLHAAVR-LKACΞ-LAASEGLSSAQPNG 171

G PTP GP + P + A S +P+P+P + + L S + A + P+ Sbjct: 408 GYPTPGGGPPSSPVPGKPAAS APMPSPHTPPDVSPEPLPEPΞPVPAPAPMPMPTPHS 464

Query: 172 PPEAEPRPPQSPASTASFIFSKGSRKLQLERPVSPETQ ADLQRNLVAELRSISEQR 227

PP + PP P S + S ++Q +P + Q + + + Sbjct: 465 PPADDYVPPTPPVPGKSPPATSPSPQVQPPAASTPPPSLVKLSPPQAPVGSPPPPVKTTS 524

Query: 228 PPQAPKKSPKAPPPVARKPSVGVPPPASPSYPRAEPLTAPP 268

PP AP SP PPPV SV PPP S P P+ +PP Sbjct: 525 PP-APIGSPSPPPPV SVVSPPPPVKSPPPPAPVGSPP 560

Pedant information for DKFZphmcfl_lc23, frame 1

Report for DKFZphmcfl_lc23.1

[LENGTH] 311

[MW] 31534.58

[pi] 9.48

[KW] All Alpha

[KW] LOW COMPLEXITY 38.59 %

SEQ MADFPPPEEAFFSVASPEPAGPSGSPELVSSPAASSSSATALQIQPPGSPDPPPAPPAPA

SEG xxxxxxxxxxxxxxx..xxxxxxxxxxxx....xxxxxxxxxxxxxxx

PRD ccccccccccccccccccccccccccccccccccccccceeeeecccccccccccccccc

SEQ PASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTPAL

SEG xxxxxx xxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccchhhhhhhhhhhccccccccccccc

SEQ GPSAPQKPLRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEAEPRPP

SEG xxxxx xxxxxxxxxxxxx

PRD cccccchhhhhhhhhcccccccccchhhhhhhhhhhhhhhhccccccccccccccccccc

SEQ QSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRΞISEQRPPQAPKKSPKAPP

SEG xxxxx xxxxxxxxxxxxxxx

PRD ccccccceeeecccchhhhhccccccchhhhhhhhhhhhhhhhccccccccccccccccc

SEQ PVARKPSVGVPPPASPSYPRAEPLTAPPTNGLPHTQDRTKRELAENGGVLQLVGPEEKMG

SEG xxxxxxxxxxxxxxxxxxxxxxx

PRD ccccccccccccccccccccccccccccccccccccchhhhhhhcccceeeccccccccc

SEQ LPGSDΞQKELA SEG

PRD ccccccccccc

(No Prosite data available for DKFZphmcfl_lc23.1) (No Pfam data available for DKFZphmcfl_lc23.1)

DKFZphmcfl_lel5

group: transmembrane protein

DKFZphmcfl_lel5 encodes a novel 454 ammo acid protein with similarity to C. elegans proteins and transporter proteins .

The novel protein is similar to the PTR2 family of proton/oligopeptide symporter proteins and the D-xylose-proton symporter. Thus, the protein is a transporter of a so far unknown compound .

The new protein can find application as a new transporter in eukaryotic cells, e.g. in drug transport into cells. similarity to D-XYLOΞE TRANSPORTER membrane regions : 9 complete cDNA, complete eds, EST hits matchs cDNA encoding cell growth inhibiting factor (E12646)

Sequenced by DKFZ

Locus : unknown

Insert length: 1957 bp

Poly A stretch at pos. 1947, polyadenylation signal at pos. 1929

1 GGTGCAGCGC CCGGGCTGAG CGACAGCAAG TGCAGCGGGC TCCTACCCCG 51 GGTGAGGGGT GGCCTCCGCG TGGGATCGTG CCCTCTTCAG CCCGCTCCTG

101 TCCCCGACAT CACGTGTATT CCGCACGTCC CCTCCGCGCT GTGTGTCTAC

151 TGAGACGGGG AGGCGTGACA GGGCCCGGGT CCCTTCTCAG TGGTGCTCTG

201 TGCTTCAGGG CAAGCTCCCC GTCTCCGGGC GCACTTCCCT CGCCTGTGTT

251 CGGTCCATCC TCCTTTCTCC AGCCTCCTCC CCTCGCAGGT GGGATCGTCG

301 GTGGGACCGG AGCGCGGGCG GGCGCGGCCC CCCGGGACCA TGGCCGGGTC

351 CGACACCGCG CCCTTCCTCA GCCAGGCGGA TGACCCGGAC GACGGGCCAG

401 TGCCTGGCAC CCCGGGGTTG CCAGGGTCCA CGGGGAACCC GAAGTCCGAG

451 GAGCCCGAGG TCCCGGACCA GGAGGGGCTG CAGCGCATCA CCGGCCTGTC

501 TCCCGGCCGT TCGGCTCTCA TAGTGGCGGT GCTGTGCTAC ATCAATCTCC

551 TGAACTACAT GGACCGCTTC ACCGTGGCTG TGTTCATCTC CAGTTACATG

601 GTGTTGGCAC CTGTGTTTGG CTACCTGGGT GACAGGTACA ATCGGAAGTA

651 TCTCATGTGC GGGGGCATTG CCTTCTGGTC CCTGGTGACA CTGGGGTCAT

701 CCTTCATCCC CGGAGAGCAT TTCTGGCTGC TCCTCCTGAC CCGGGGCCTG

751 GTGGGGGTCG GGGAGGCCAG TTATTCCACC ATCGCGCCCA CTCTCATTGC

801 CGACCTCTTT GTGGCCGACC AGCGGAGCCG GATGCTCAGC ATCTTCTACT

851 TTGCCATTCC GGTGGGCAGT GGTCTGGGCT ACATTGCAGG CTCCAAAGTG

901 AAGGATATGG CTGGAGACTG GCACTGGGCT CTGAGGGTGA CACCGGGTCT

951 AGGAGTGGTG GCCGTTCTGC TGCTGTTCCT GGTAGTGCGG GAGCCGCCAA 1001 GGGGAGCCGT GGAGCGCCAC TCAGATTTGC CACCCCTGAA CCCCACCTCG 1051 TGGTGGGCAG ATCTGAGGGC TCTGGCAAGA AATCTCATCT TTGGACTCAT 1101 CACCTGCCTG ACCGGAGTCC TGGGTGTGGG CCTGGGTGTG GAGATCAGCC 1151 GCCGGCTCCG CCACTCCAAC CCCCGGGCTG ATCCCCTGGT CTGTGCCACT 1201 GGCCTCCTGG GCTCTGCACC CTTCCTCTTC CTGTCCCTTG CCTGCGCCCG 1251 TGGTAGCATC GTGGCCACTT ATATTTTCAT CTTCATTGGA GAGACCCTCC 1301 TGTCCATGAA CTGGGCCATC GTGGCCGACA TTCTGCTGTA CGTGGTGATC 1351 CCTACCCGAC GCTCCACCGC CGAGGCCTTC CAGATCGTGC TGTCCCACCT 1401 GCTGGGTGAT GCTGGGAGCC CCTACCTCAT TGGCCTGATC TCTGACCGCC 1451 TGCGCCGGAA CTGGCCCCCC TCCTTCTTGT CCGAGTTCCG GGCTCTGCAG 1501 TTCTCGCTCA TGCTCTGCGC GTTTGTTGGG GCACTGGGCG GCGCAGCCTT 1551 CCTGGGCACC GCCATCTTCA TTGAGGCCGA CCGCCGGCGG GCACAGCTGC 1601 ACGTGCAGGG CCTGCTGCAC GAAGCAGGGT CCACAGACGA CCGGATTGTG 1651 GTGCCCCAGC GGGGCCGCTC CACCCGCGTG CCCGTGGCCA GTGTGCTCAT 1701 CTGAGAGGCT GCCGCTCACC TACCTGCACA TCTGCCACAG CTGGCCCTGG 1751 GCCCACCCCA CGAAGGGCCT GGGCCTAACC CCTTGGCCTG GCCCAGCTTC 1801 CAGAGGGACC CTGGGCCGTG TGCCAGCTCC CAGACACTAC ATGGGTAGCT 1851 CAGGGGAGGA GGTGGGGGTC CAGGAGGGGG ATCCCTCTCC ACAGGGGCAG 1901 CCCCAAGGGC TCGGTGCTAT TTGTAACGGA ATAAAATTTG TAGCCAGAAA 1951 AAAAAAA

BLAST Results

Entry E12646 from database EMBL: cDNA encoding cell growth inhibiting factor.

Score = 3046, P = 2.2e-131, identities = 640/659 Medline entries

No Medline entry

Peptide information for frame 1

ORF from 340 bp to 1701 bp; peptide length: 454 Category: similarity to known protein

1 MAGΞDTAPFL SQADDPDDGP VPGTPGLPGS TGNPKSEEPE VPDQEGLQRI

51 TGLSPGRSAL IVAVLCYINL LNYMDRFTVA VFISSYMVLA PVFGYLGDRY

101 NRKYLMCGGI AFWSLVTLGS SFIPGEHFWL LLLTRGLVGV GEASYSTIAP

151 TLIADLFVAD QRSRMLSIFY FAIPVGSGLG YIAGSKVKDM AGDWHWALRV

201 TPGLGVVAVL LLFLVVREPP RGAVERHSDL PPLNPTSWWA DLRALARNLI

251 FGLITCLTGV LGVGLGVEIS RRLRHSNPRA DPLVCATGLL GSAPFLFLSL

301 ACARGSIVAT YIFIFIGETL LSMNWAIVAD ILLYVVIPTR RSTAEAFQIV

351 LSHLLGDAGS PYLIGLISDR LRRNWPPSFL SEFRALQFSL MLCAFVGALG

401 GAAFLGTAIF IEADRRRAQL HVQGLLHEAG STDDRIVVPQ RGRSTRVPVA 451 SVLI

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphmcfl_lel5, frame 1

TREMBL :CEC13C4_1 gene: "C13C4.5"; Caenorhabditis elegans cosmid C13C4, N = 3, Score = 441, P = 5.2e-76

TREMBL :CEC39E9_10 gene: "C39E9.10"; Caenorhabditis elegans cosmid C39E9, N = 2, Score = 449, P = 8.2e-69

TREMBL :CEF09A5_1 gene: "F09A5.1"; Caenorhabditis elegans cosmid F09A5, N = 3, Score = 413, P = 9.1e-60

TREMBL:ATF6H11_18 gene: "F6H11.180"; product: "predicted protein"; Arabidopsis thaliana DNA chromosome 5, BAC clone F6H11 (ESSAII project), N = 3, Score = 193, P = 2.5e-24

SWISSPROT :XYLT_LACBR D-XYLOSE-PROTON SYMPORT (D-XYLOSE TRANSPORTER)., N = 1, Score = 180, P = 7.9e-ll

>TREMBL:CEC39E9_10 gene: "C39E9.10"; Caenorhabditis elegans cosmid C39E9 Length = 488

HSPs:

Score = 449 (67.4 bits), Expect = 8.2e-69, Sum P(2) = 8.2e-69 Identities = 88/204 (43%), Positives = 125/204 (61%)

Query: 58 SALIVAVLCYINLLNYMDRFTVAVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVT 117

+ ++ V Y N+ + + VF+ S+MV +PV GYLGDR+NRK++M G+ W Sbjct: 29 AGVLTQVQTYYNISDSLGGLIQTVFLISFMVFSPVCGYLGDRFNRKWIMIIGVGIWLGAV 88

Query: 118 LGSSFIPGEHFWLLLLTRGLVGVGEASYSTIAPTLIADLFVADQRSRMLSIFYFAIPVGS 177

LGSΞF+P HFWL L+ R VG+GEASYS +AP+LI+D+F +RS + IFYFAIPVGS Sbjct: 89 LGSSFVPANHFWLFLVLRSFVGIGEASYSNVAPSLISDMFNGQKRSTVFMIFYFAIPVGS 148

Query: 178 GLGYIAGSKVKDMAGDWHWALRVTPGLGVVAVLLLFLVVREPPRGAVER HSDLPPL 233

GLG+I GS V + G W W +RV+ G++ ++ L L EP RGA ++ D+ Sbjct: 149 GLGFIVGSNVATLTGHWQWGIRVSAIAGLIVMIALVLFTYEPERGAADKAMGESKDVVVT 208

Query: 234 NPTSWWADLRALARNLIFGLITCLTG 259

T++ DL L + L+ C G Sbjct: 209 TNTTYLEDLVILLKTPT—LVACTWG 232

Score = 267 (40.1 bits), Expect = 8.2e-69, Sum P(2) = 8.2e-69 Identities = 74/212 (34%), Positives = 113/212 (53%)

Query: 249 LIFGLITCLTGVLGVGLGVEISRRL RHSNPRADPLVCATGLLGSAPFLFLSL 300

L FG IT G++GV G +S+ L R RA PLV G L +APFL + + Sbjct: 277 LYFGAITTAGGLIGVIFGSMLSKWLVAGWGPFRRLQTDRAQPLVAGGGALLAAPFLLIGM 336

Query: 301 ACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGS 360 S+V YI IF G T + NW + D+L V+ P RRSTA ++ +++SHL GDA Sbjct: 337 IFGDKSLVLLYIMIFFGITFMCFNWGLNIDMLTTVIHPNRRSTAFSYFVLVSHLFGDASG 396

Query: 361 PYLIGLISDRLRRN—WPPSFLΞEFRALQFSLMLCAFVGALGGAAFLGTAIFIEADRR— 416

PYLIGLISD +R +P ++ +L + C + L + +++ + +DR+ Sbjct: 397 PYLIGLIΞDAIRHGSTYPKD QYHSLVSATYCCVALLLLSAGLYFVSSLTLVΞDRKKF 453

Query: 417 RAQLHVQGLLHEA—GSTD—DRIVVPQRGRSTRV 447

RA++ + L + STD +RI + S+R+ Sbjct: 454 RAEMGLDDLQΞKPIRTSTDSLERIGINDDVASSRL 488

Score = 70 (10.5 bits), Expect = 5.9e-24, Sum P(2) = 5.9e-24 Identities = 25/89 (28%), Positives = 41/89 (46%)

Query: 62 VAVLCYINLLNYMDRFTVAVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVT—LG 119

V L +NLLNY+DR+TVA ++ + LG +L+ +S V LG Sbjct: 11 VTALFVVNLLNYVDRYTVAGVLTQVQTYYNISDSLGGLIQTVFLI—SFMVFSPVCGYLG 68

Query: 120 SSFIPGEHFWLLLLTRGLVGVGEASYSTIAP 150

F W++++ G + +G S+ P Sbjct: 69 DRF NRKWIMIIGVG-IWLGAVLGSSFVP 95

Pedant information for DKFZphmcfl_lel5, frame 1

Report for DKFZphmcfl_lel5.1

[LENGTH] 454

[MW] 49013.35

[pi] 7.66

[HOMOL] TREMBL :CEC13C4_1 gene: "C13C4.5"; Caenorhabditis elegans cosmid C13C4 2e-51

[BLOCKS] BL01022D

[PROSITE] MYRISTYL 11

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 3

[PROSITE] PROKAR_LIPOPROTEIN 1

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE 4

[KW] TRANSMEMBRANE 8

[KW] LOW_COMPLEXITY 15.42 %

SEQ MAGSDTAPFLSQADDPDDGPVPGTPGLPGSTGNPKSEEPEVPDQEGLQRITGLSPGRSAL

SEG xxxxxxxxxxxxxxxx

PRD cccccceeeeeecccccccccccccccccccccccccccccccccceeeecccccchhhh

MEM MMMMMMMMMMMMMMMMMMMMMMM

SEQ IVAVLCYINLLNYMDRFTVAVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVTLGS

SEG

PRD hhhhhhhhccccccccceeeeeehhhhheeeecccccccccceeeeeeeccceeeeeecc

MEM MMMMMM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ SFIPGEHFWLLLLTRGLVGVGEASYSTIAPTLIADLFVADQRΞRMLSIFYFAIPVGSGLG

SEG xxxxxxxxxxxx

PRD cccccchhhhhhhhhhccccccceeeeecceeeccccccccchhhhheeeeeecccccce

MEM MMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMM

SEQ YIAGΞKVKDMAGDWHWALRVTPGLGVVAVLLLFLVVREPPRGAVERHSDLPPLNPTSWWA

SEG xxxxxxxxxxxxx

PRD eeecccccccccccceeeeeeccchhhhhhhhhhhhcccccchhhhhccccccccccchh

MEM MMMMMMMMM

SEQ DLRALARNLIFGLITCLTGVLGVGLGVEISRRLRHSNPRADPLVCATGLLGSAPFLFLSL

SEG xxxxxxxxxxxxxxxx

PRD hhhhhhhhhhhhheeeecccceeehhhhhhhhhhccccccceeecccceeeecccceeec

MEM MMMMMMMMMMMMMMMMMMMMMMMMM

SEQ ACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGS

SEG

PRD ccccchhhhheeeeeeccccccccchhhhhhheeeeeccccchhhhhhcccccccccccc

MEM MMMM MMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMM

SEQ PYLIGLISDRLRRNWPPSFLSEFRALQFSLMLCAFVGALGGAAFLGTAIFIEADRRRAQL

SEG xxxxxxxxxxxxx

PRD ceeehhhhhhhhcccccchhhhhhhhhhhhhhhhhhhhhhhccccceeeeeehhhhhhhh

MEM MMMMMMMM MM

SEQ HVQGLLHEAGSTDDRIVVPQRGRSTRVPVASVLI SEG

PRD hhhhhhhhccccceeeeeeccccccceeeeeccc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMM

Prosite for DKFZphmcfl_lel5.1

PS00002 177->181 GLYCOSAMINOGLYCAN PDOC00002

PS00004 340->344 CAMP_PHOSPHO_SITE PDOC00004

PS00005 270->273 PKC_PHOSPHO_SITE PDOC00005

PS00005 339->342 PKC_PHOSPHO_SITE PDOC00005

PS00005 368->371 PKC_PHOSPHO_SITE PDOC00005

PS00005 444->447 PKC_PHOSPHO_SITE PDOC00005

PS00006 11->15 CK2_PHOSPHO_SITE PDOC00006

PS00006 342->346 CK2_PHOSPHO_SITE PDOC00006

PS00006 431->435 CK2_PHOΞPHO_SITE PDOC00006

PS00008 26->32 MYRISTYL PDOC00008

PS00008 32->38 MYRISTYL PDOC00008

PS00008 52->58 MYRISTYL PDOC00008

PS00008 139->145 MYRISTYL PDOC00008

PS00008 176->182 MYRISTYL PDOC00008

PS00008 252->258 MYRISTYL PDOC00008

PS00008 262->268 MYRISTYL PDOC00008

PS00008 266->272 MYRISTYL PDOC00008

PS00008 288->294 MYRISTYL PDOC00008

PS00008 305->311 MYRISTYL PDOC00008

PS00008 397->403 MYRISTYL PDOC00008

PΞ00013 292->303 PROKAR LIPOPROTEIN PDOC00013

(No Pfam data available for DKFZphmcfl_lel5.1)

DKFZphmcfl_lgl3

group: mammary carcinoma derived

DKFZphmcfl_lgl3 encodes a novel 573 amino acid protein with very weak similarity to the human KIAA0543 protein and Musca domestica hermes transposase.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of mammary carcinoma- specific genes. similarity to KIAA0766 commplete cDNA, complete eds, few EST hits on genomic level encoded by AC005020, no splicing, genomic?

Sequenced by DKFZ

Locus : unknown

Insert length: 2210 bp

Poly A stretch at pos. 2200, polyadenylation signal at pos. 2176

1 GAAACCTGAT CTCATAAAAC CTAGGTCACA AAGGACAGCC CTGCAAAACA

51 GACCCTATTT GGATCAAGTG AGCCAGTTCC TGGAACCTGA ATAATGACTC

101 CTGAATCAAG GGATACTACA GATTTGTCTC CAGGGGGTAC CCAGGAGATG

151 GAAGGCATCG TGATAGTGAA GGTGGAGGAG GAAGATGAAG AAGACCATTT

201 TCAAAAGGAA AGAAACAAAG TAGAGTCATC GCCACAAGTT CTCAGTCGCT

251 CTACAACTAT GAATGAGAGA GCCTTATTGT CATCGTATTT AGTTGCATAT

301 AGAGTGGCAA AAGAGAAAAT GGCTCACACA GCGGCTGAAA AAATTATCCT

351 TCCAGCATGT ATGGACATGG TACGGACAAT TTTTGATGAC AAATCAGCTG

401 ATAAACTAAG AACTATACCT CTTAGTGATA ATACAATATC TCGTCGAATC

451 TGTACGATTG CAAAACATTT GGAAGCAATG CTTATTACAC GGCTGCAGTC

501 CGGTATAGAC TTTGCAATCC AACTCGATGA GAGCACTGAT ATTGCAAGTT

551 GTCCCACACT CTTGGTTTAT GTCAGATATG TGTGGCAAGA TGATTTTGTA

601 GAGGATCTCT TATGTTGTTT AAATTTAAAT TCACATATAA CTGGATTAGA

651 TTTATTTACT GAATTAGAAA ACTGCCTTCT TGGTCAGTAT AAATTAAACT

701 GGAAACATTG TAAAGGAATT TCAAGTGATG GAACAGCAAA TATGACCGGA

751 AAACACAGCA GACTTACTGA AAAATTGTTA GAAGCAACCC ACAACAATGC

801 TGTTTGGAAT CACTGTTTTA TTCATCGAGA AGCTTTGGTA TCCAAAGAAA

851 TTTCACCAAG TCTGATGGAT GTATTGAAAA ATGCAGTGAA AACTGTTAAT

901 TTTATTAAAG GAAGCTCACT GAATAGCCGA CTTCTCGAAA TATTTTGTTC

951 AGAGATTGGA GTGAACCACA CCCACTTATT GTTTCATACA GAAGTTCGTT

1001 GGCTTTCTCA AGGAAAAGTA TTGAGCAGAG TATATGAACT CAGGAACGAG

1051 ATTTACATTT TTCTCGTTGA AAAGCAATCT CATTTGGCAA ATATTTTTGA

1101 AGACGACATT TGGGTAACAA AATTGGCATA TTTAAGTGAT ATTTTTGGCA

1151 TTCTTAATGA ATTAAGCCTG AAAATGCAGG GGAAAAACAA TGATATATTT

1201 CAGTATCTTG AACATATTCT AGGATTCCAA AAGACGTTAT TATTGTGGCA

1251 AGCAAGACTT AAAAGTAACC GCCCTAGCTA CTATATGTTT CCAACATTAT

1301 TGCAACACAT CGAAGAGAAC ATTATTAATG AAGACTGCTT AAAAGAAATA

1351 AAA AGAGA TATTGTTGCA TCTCACTTCT TTGTCTCAAA CTTTTAATTA

1401 TTACTTTCCG GAAGAGAAAT TTGAATCATT AAAGGAAAAT ATTTGGATGA

1451 AAGATCCATT TGCTTTTCAA AACCCAGAAT CAATAATTGA GTTAAACTTG

1501 GAGCCTGAAG AAGAGAATGA ATTATTGCAG CTCAGTTCAT CATTCACACT

1551 AAAGAATTAT TATAAGATAT TAAGTTTATC AGCATTTTGG ATTAAGATTA

1601 AAGATGACTT TCCACTGCTA AGTAGGAAGA GTATATTGCT GTTACTACCA

1651 TTCACAACTA CATATTTGTG TGAACTAGGA TTTTCAATCT TGACACGGTT

1701 AAAAACAAAG AAGAGAAATA GGCTCAATAG TGCACCAGAT ATGCGGGTAG

1751 CATTATCTTC ATGTGTTCCT GACTGGAAGG AACTTATGAA CAGACAAGCA

1801 CACCCATCAC ATTAAATACA AACTTTACAA AATTCTGTGT ATAGCCAGGT

1851 GTGGTGGCTT ACGCCTGTAA TCCCAGCAGT GGGAGACCGA GGTGGGCAGA

1901 TCACTTGAGT TCAAGACCAG CCTGGCCAAC ATGGTGAAAC CCCATCTCTA

1951 CTAAAAATAG AAACCTTAGC CAGGCGTGGT GGCACATGCC TGCAGTCCCA

2001 GTTACTTGGG TGCCTGAGGC AGGAGAATCT CTTAAACCAG GAAGGCAGAG

2051 ATTGCAGTGA GCTGAGATAA TCCCACTGCA TTCCAGCCTG GGCAACAGCG

2101 TGAGACTTCA TCTCAAAAAA AAAAAATTGT ATTTGTACTT TTAAAGGGAT

2151 TTTGCAGTAT GTTGTAGTTA AACGTTAATA AAATTATATT TGTAATTAGG

2201 AAAAAAAAAA

BLAST Results

Entry AC005020 from database EMBL:

Homo sapiens clone GS259H13; HTGS phase 1, 4 unordered pieces.

Score = 9110, P = O.Oe+00, identities = 1822/1822 Medline entries

No Medline entry

Peptide information for frame 1

ORF from 94 bp to 1812 bp; peptide length: 573 Category: similarity to unknown protein

1 MTPESRDTTD LSPGGTQEME GIVIVKVEEE DEEDHFQKER NKVESSPQVL

51 SRSTTMNERA LLSSYLVAYR VAKEKMAHTA AEKIILPACM DMVRTIFDDK

101 SADKLRTIPL SDNTISRRIC TIAKHLEAML ITRLQSGIDF AIQLDESTDI

151 ASCPTLLVYV RYVWQDDFVE DLLCCLNLNS HITGLDLFTE LENCLLGQYK

201 LNWKHCKGIS SDGTANMTGK HSRLTEKLLE ATHNNAVWNH CFIHREALVS

251 KEISPSLMDV LKNAVKTVNF IKGSSLNSRL LEIFCSEIGV NHTHLLFHTE

301 VRWLSQGKVL SRVYELRNEI YIFLVEKQSH LANIFEDDIW VTKLAYLSDI

351 FGILNELSLK MQGKNNDIFQ YLEHILGFQK TLLLWQARLK SNRPSYYMFP

401 TLLQHIEENI INEDCLKEIK LEILLHLTSL SQTFNYYFPE EKFESLKENI

451 WMKDPFAFQN PESIIELNLE PEEENELLQL SSSFTLKNYY KILΞLSAFWI

501 KIKDDFPLLS RKSILLLLPF TTTYLCELGF SILTRLKTKK RNRLNSAPDM

551 RVALSSCVPD WKELMNRQAH PSH

BLASTP hits

Entry AC004877_3 from database TREMBLNEW: gene: "WUGSC :H_DJ0751H13.2"; product: "KIAA0543 protein"; Homo sapiens

PAC clone DJ0751H13 from 7q35-qter, complete sequence.

Score = 86, P = 4.4e-03, identities = 46/179, positives = 78/179

Entry MD36211_1 from database TREMBL: product: "Hermes transposase"; Musca domestica Hermes transposase gene, complete eds.

Score = 105, P = 3.0e-02, identities = 101/465, positives = 202/465

Alert BLASTP hits for DKFZphmcfl_lgl3, frame 1

TREMBL :AB018309_1 gene: "KIAA0766"; product: "KIAA0766 protein"; Homo sapiens mRNA for KIAA0766 protein, complete eds., N = 1, Score = 300, P = l.le-23

>TREMBL:AB018309_1 gene: "KIAA0766"; product: "KIAA0766 protein"; Homo sapiens mRNA for KIAA0766 protein, complete eds. Length = 607

HSPs:

Score = 300 (45.0 bits), Expect = l.le-23, P = l.le-23 Identities = 120/485 (24%), Positives = 229/485 (47%)

Query: 89 CMD-MVRTIFDDKSADKLRTIPLSDNTISRRICTIAKHLEAMLITRLQSGIDFAIQLDES 147

CM+ ++R + + L+ + LS + +RI +1 ++L L R + +++ LD+ Sbjct: 124 CMEVLLREVLPEH-VSVLQGVDLSPDITRQRILSIDRNLRNQLFNRARDFKAYSLALDDQ 182

Query: 148 TDIASCPTLLVYVRYVWQD-DFVEDLLCCLNLNSHIT-GLDLFTELENCLLGQYKLNWKH 205

+A LLV++R V + + EDLL +NL H + G + LE+ L L+ + Sbjct: 183 AFVAYENYLLVFIRGVGPELEVQEDLLTIINLTHHFSVGALMSAILES—LQTAGLSLQR 240

Query: 206 CKGISSDGTANMTGKHSRLTEKLLEATHNNAVWN—HC—FIHREALVSKEISPSLMDVL 261

G+++ T M G++S L + E + WN H F+H E L S ++ + ++ Sbjct: 241 MVGLTTTHTLRMIGENSGLVSYMREKAVSPNCWNVIHYSGFLHLELLSSYDVDVN—QII 298

Query: 262 KNAVKTVNFIKGSSLNSRLLEIFCSEIGVNHTHLLFHTEVR-WLSQGKVLSRVYELRNEI 320

+ + IK + + +E H + + WL +GK L ++ LR E+ Sbjct: 299 NTISEWIVLIKTRGVRRPEFQTLLTESESEHGERVNGRCLNNWLRRGKTLKLIFSLRKEM 358

Query: 321 YIFLVEKQSHLANIFEDDIWVTKLAYLSDIFGILNELSLKMQGKNNDIFQYLEHILGFQK 380

FLV + + F D W+ +L Dl L ELS +++ +HI F+

Sb_jct: 359 EAFLVSVGATTVH-FSDKQWLCDFGFLVDIMEHLRELSEELRVSKVFAAAAFDHICTFEV 417 Query: 381 TLLLWQARLKSNRPSYYMFPTLLQHIEE NIINEDCLKEIKLEILLHLTSLSQTFNY 436

L L+Q ++ + FP L + ++E N +E + ++++ L + F

Sbjct: 418 KLNLFQRHIEEKNLTD—FPALREVVDELKQQNKEDEKIFDPDRYQMVI—CRLQKEFER 473

Query: 437 YFPEEKFESLKENIWM-KDPFAFQNPESIIELNLEPEEENELLQLSSSFTLKNYYKILSL 495 +F + +F +K+++ + +PF F+ + I + +E L +L ++ L N Y+I L

Sbjct: 474 HFKDLRF—IKKDLELFSNPFNFKPEYAPISVRVE LTKLQANTNLWNEYRIKDL 525

Query: 496 SAFWIKIK-DDFPLLSRKSILLLLPFTTTYLCELGFSILTRLKTKKRNRLNSA PDMR 551

F+ + + +P++ + + F + +CE FS LTR + L R

Sbjct: 526 GQFYAGLSAESYPIIKGVACKVASLFDSNQICEKAFSYLTRNQHTLSQPLTDEHLQALFR 585

Query: 552 VALSSCVPDWKELMNRQAHPSH 573 VA + P W +L+ R+ + S+

Sbjct: 586 VATTEMEPGWDDLV-RERNESN 606

Score ^: = 290 (43.5 bits), Expect = 1.5e-22, P = 1.5e-22

Identities ^:= 120/485 (24%), Positives = 228/485 (47%)

Query: 89 CMD-MVRTIFDDKSADKLRTIPLSDNTISRRICTIAKHLEAMLITRLQSGIDFAIQLDES 147 CM+ ++R + + L+ + LS + +RI +1 ++L L R + +++ LD+

Sbjct: 124 CMEVLLREVLPEH-VSVLQGVDLSPDITRQRILSIDRNLRNQLFNRARDFKAYSLALDDQ 182

Query: 148 TDIASCPTLLVYVRYVWQD-DFVEDLLCCLNLNSHIT-GLDLFTELENCLLGQYKLNWKH 205

+A LLV++R V + + EDLL +NL H + G + LE+ L L+ +

Sbjct: 183 AFVAYENYLLVFIRGVGPELEVQEDLLTIINLTHHFSVGALMΞAILES—LQTAGLSLQR 240

Query: 206 CKGISSDGTANMTGKHSRLTEKLLEATHNNAVWNHCFIHREALVSKEISPSLMDV-LKNA 264

G+++ T M G++S L + E + WN IH + E+ S DV +

Sbjct: 241 MVGLTTTHTLRMIGENSGLVSYMREKAVSPNCW —VIHYSGFLHLELLSSY-DVDVNQI 297

Query: 265 VKTVN FIKGSSLNSRLLEIFCSEIGVNHTHLLFHTEVR-WLSQGKVLSRVYELRNE 319

+ T++ IK + + +E H + + WL +GK L ++ LR E

Sbjct: 298 INTISEWIVLIKTRGVRRPEFQTLLTESESEHGERVNGRCLNNWLRRGKTLKLIFSLRKE 357

Query: 320 IYIFLVEKQSHLANIFEDDIWVTKLAYLSDIFGILNELSLKMQGKNNDIFQYLEHILGFQ 379 + FLV + + F D W+ +L Dl L ELS +++ +HI F+

Sbjct: 358 MEAFLVSVGATTVH-FSDKQWLCDFGFLVDIMEHLRELSEELRVSKVFAAAAFDHICTFE 416

Query: 380 KTLLLWQARLKSNRPSYYMFPTLLQHIEENIINEDCLKEIKL EILLHLTSLSQTFN 435

L L+Q ++ + FP L + ++E + + ++ K+ + + L + F

Sbjct: 417 VKLNLFQRHIEEKNLTD—FPALREVVDE—LKQQNKEDEKIFDPDRYQMVICRLQKEFE 472

Query: 436 YYFPEEKFESLKENIWM-KDPFAFQNPESIIELNLEPEEENELLQLSSSFTLKNYYKILS 494

+F + +F +K+++ + +PF F+ + I + +E L +L ++ L N Y+I

Sbjct: 473 RHFKDLRF—IKKDLELFSNPFNFKPEYAPISVRVE LTKLQANTNLWNEYRIKD 524

Query: 495 LSAFWIKIK-DDFPLLSRKSILLLLPFTTTYLCELGFSILTRLKTKKRNRLNSA PDM 550

L F+ + + +P++ + + F + +CE FS LTR + L

Sbjct: 525 LGQFYAGLSAESYPIIKGVACKVASLFDSNQICEKAFSYLTRNQHTLSQPLTDEHLQALF 584

Query: 551 RVALSSCVPDWKELMNRQAHPSH 573 RVA + P W +L+ R+ + S+

Sbjct: 585 RVATTEMEPGWDDLV-RERNESN 606

Pedant information for DKFZphmcfl_lgl3, frame 1

Report for DKFZphmcf1 lgl3.1

[LENGTH] 573

[MW] 66276.85

[pi] 5.82

[HOMOL] TREMBL :AB018309_1 gene: "KIAA0766" product: "KIAA0766 protein"; Homo sapiens mRNA for KIAA0766 protein, complete eds. le-18

[PROSITE] MYRISTYL 3

[PROSITE] CK2_PHOSPHO_SITE 10

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 9

[PROSITE] AΞN_GLYCOSYLATION 2

[KW] All_Alpha

[KW] LOW COMPLEXITY 8.90 %

SEQ MTPESRDTTDLSPGGTQEMEGIVIVKVEEEDEEDHFQKERNKVESSPQVLSRSTTMNERA SEG xxxxxxx

PRD ccccccccccccccccccceeeeeeeeccccchhhhhhhhhhcccccceeecccchhhhh

SEQ LLSSYLVAYRVAKEKMAHTAAEKI ILPACMDMVRTI FDDKSADKLRTI PLSDNTISRRIC SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhchhhhhhhhhhcccccceeeeecccchhhhhhh

SEQ TIAKHLEAMLITRLQSGIDFAIQLDESTDIASCPTLLVYVRYVWQDDFVEDLLCCLNLNS SEG PRD hhhhhhhhhhhhhhhhhheeeccccccccccccccceeeeeeeccchhhhhhhhhhccce

SEQ HITGLDLFTELENCLLGQYKLNWKHCKGISSDGTANMTGKHSRLTEKLLEATHNNAVWNH SEG PRD eeeehhhhhhhhhhhhhhhccccccccccccccceeeecccchhhhhhhhhhccccceee

SEQ CFIHREALVSKEISPSLMDVLKNAVKTVNFIKGSSLNSRLLEIFCSEIGVNHTHLLFHTE SEG PRD hhhhhhhhhhhhcccchhhhhhhhhhhheeecccccchhhhhhhhhhccccchhhhhhhh

SEQ VRWLSQGKVLSRVYELRNEIYIFLVEKQSHLANIFEDDIWVTKLAYLSDIFGILNELSLK SEG PRD cccccccchhhhhhhhhhhhhhhhhhhhchhhhhcccceeehhhhhhhhhhhhhhhhhhh

SEQ MQGKNNDIFQYLEHILGFQKTLLLWQARLKSNRPSYYMFPTLLQHIEENIINEDCLKEIK

SEG xxxxx

PRD hhccccccchhhhhhhhhhhhhhhhhhhhhcccccccccchhhhhhhhhhhhcchhhhhh

SEQ LEILLHLTSLSQTFNYYFPEEKFESLKENIWMKDPFAFQNPEΞIIELNLEPEEENELLQL

SEG xxxxx xxxxxxxxxxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhccchhhhhhhhhhhhhcccccccccccceeecccchhhhhhhhh

SEQ SSSFTLKNYYKILSLSAFWIKIKDDFPLLSRKSILLLLPFTTTYLCELGFSILTRLKTKK SEG XXX xxxxxxxxxxx PRD hhcccchhhhhhhhhhhhhcccccccccchhhhhhhhhccceeeeehhhhhhhhhhhhhh

SEQ RNRLNSAPDMRVALSSCVPDWKELMNRQAHPSH SEG PRD hcccccccccceeeccccccchhhhhhhccccc

Prosite for DKFZphmcfl_lgl3.1

PS00001 216->220 ASN_GLYCOSYLATION PDOC00001 PS00001 291->295 AΞN_GLYCOSYLATION PDOC00001 PS00005 116->119 PKC_PHOSPHO_SITE PDOC00005 PS00005 218->221 PKC_PHOSPHO_SITE PDOC00005 PS00005 225->228 PKC_PHOSPHO_SITE PDOC00005 PS00005 358->361 PKC_PHOSPHO_SITE PDOC00005 PS00005 391->394 PKC_PHOSPHO_SITE PDOC00005 PS00005 445->448 PKC_PHOSPHO_SITE PDOC00005 PS00005 485->488 PKC_PHOSPHO_SITE PDOC00005 PS00005 510->513 PKC_PHOSPHO_SITE PDOC00005 PS00005 538->541 PKC_PHOSPHO_SITE PDOC00005 PS00006 55->59 CK2_PHOSPHO_SITE PDOC00006 PS00006 79->83 CK2_PHOSPHO_SITE PDOC00006 PS00006 95->99 CK2_PHOSPHO_SITE PDOC00006 PS00006 136->140 CK2_PHOSPHO_SITE PDOC00006 PS00006 183->187 CK2_PHOSPHO_SITE PDOC00006 PS00006 189->193 CK2_PHOSPHO_SITE PDOC00006 PS00006 256->260 CK2_PHOSPHO_SITE PDOC00006 PS00006 445->449 CK2_PHOSPHO_SITE PDOC00006 PS00006 463->467 CK2_PHOSPHO_SITE PDOC00006 PS00006 546->550 CK2_PHOSPHO_SITE PDOC00006 PS00007 364->372 TYR_PHOSPHO_SITE PDOC00007 PS00008 137->143 MYRISTYL PDOC00008 PS00008 273->279 MYRISTYL PDOC00008 PS00008 289->295 MYRISTYL PDOC00008

(No Pfam data available for DKFZphmcfl_lgl3.1) DKFZphtes3_14g5

group: testes derived

DKFZphtes3_14g5 encodes a novel 379 amino acid protein with strong similarity to murine cell growth regulating nucleolar protein LYAR.

The novel protein is very similar to murine Ly-1 antibody reactive clone protein (LYAR) . It contains a ATP/GTP-binding site motif A (P-loop, interacts with one of the phosphate groups of a ATP/GTP nucleotide), but not the zinc finger motif and and nuclear localization signals of lyar . No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . strong similarity to cell growth regulating nucleolar protein LYAR, of mouse complete cDNA, complete eds, EST hits

Sequenced by BMFZ

Locus: unknown

Insert length: 1503 bp

Poly A stretch at pos. 1467, polyadenylation signal at pos. 1440

1 CCCAGAGGTC CGACCTGGGA GGCTGGGGCT CAGAGAGCAA TGTTTGCTGT

51 CTTCCATTGG AGTGACTGAA TTTCTACATG ACGGCTTTTT GACAAGACTT

101 AAAACCTGTC TTGGATAGAG AATATTTAGC CATTTACCTA AAAATGGTAT

151 TTTTTACATG CAATGCATGT GGTGAATCAG TGAAGAAAAT ACAAGTGGAA

201 AAGCATGTGT CTGTTTGCAG AAACTGTGAA TGCCTTTCTT GCATTGACTG

251 CGGTAAAGAT TTCTGGGGCG ATGACTATAA AAACCACGTG AAATGCATAA

301 GTGAAGATCA GAAGTATGGT GGCAAAGGCT ATGAAGGTAA AACCCACAAA

351 GGCGACATCA AACAGCAGGC GTGGATTCAG AAAATTAGTG AATTAATAAA

401 GAGACCCAAT GTCAGCCCCA AAGTGAGAGA ACTTTTAGAG CAAATTAGTG

451 CTTTTGACAA CGTTCCCAGG AAAAAGGCAA AATTTCAGAA TTGGATGAAG

501 AACAGTTTAA AAGTTCATAA TGAATCCATT CTGGACCAGG TGTGGAATAT

551 CTTTTCTGAA GCTTCCAACA GCGAACCAGT CAATAAGGAA CAGGATCAAC

601 GGCCACTCCA CCCAGTGGCA AATCCACATG CAGAAATCTC CACCAAGGTT

651 CCAGCCTCCA AAGTGAAAGA CGCCGTGGAA CAGCAAGGGG AGGTGAAGAA

701 GAATAAAAGA GAAAGAAAGG AAGAACGGCA GAAGAAAAGG AAAAGAGAAA

751 AGAAAGAACT AAAGTTAGAA AACCACCAGG AAAACTCAAG GAATCAGAAG

801 CCTAAGAAGC GCAAAAAGGG ACAGGAGGCT GACCTTGAGG CTGGTGGGGA

851 GGAAGTCCCT GAGGCCAATG GCTCTGCAGG GAAGAGGAGC AAGAAGAAGA

901 AGCAGCGCAA GGACAGCGCC AGTGAGGAAG AGGCACGCGT GGGCGCAGGG

951 AAGAGGAAGC GGAGGCACTC GGAAGTTGAA ACAGATTCTA AGAAGAAAAA

1001 GATGAAGCTC CCAGAGCATC CTGAGGGCGG AGAACCAGAA GACGATGAGG

1051 CTCCTGCAAA AGGTAAATTC AACTGGAAGG GAACTATTAA AGCAATTCTG

1101 AAACAGGCCC CAGACAATGA AATAACCATC AAAAAGCTAA GGAAAAAGGT

1151 TTTAGCTCAG TACTACACAG TGACAGATGA GCATCACAGA TCCGAAGAGG

1201 AACTCCTGGT CATCTTTAAC AAGAAAATCA GCAAGAACCC TACCTTTAAG

1251 TTATTAAAGG ACAAAGTCAA GCTTGTGAAA TGAACATTTG TGTATTTAAA

1301 AATTGAATCC ATTCTGCTGA CTTCTTCCTT TCACTGCTGT TTATAAAATG

1351 TGTAATGAAT TCTAACAACT CAAATTTTGC TTTTTGAAGC TGTATTTTTA

1401 AGTTAAGAAA ATATATTTTT GGTATAACTT TTATGAGAAA AATAAAATAT

1451 ATTCTGGTCC AAACTTCAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

1501 AAA

BLAST Results

No BLAST result

Medline entries

93259460:

LYAR, a novel nucleolar protein with zinc fmger DNA-bindmg motifs, is involved in cell growth regulation. Peptide information for frame 3

ORF from 144 bp to 1280 bp; peptide length: 379 Category: strong similarity to known protein Classification: Cell division Prosite motifs: ATP GTP A (60-68)

1 MVFFTCNACG ESVKKIQVEK HVSVCRNCEC LΞCIDCGKDF WGDDYKNHVK

51 CISEDQKYGG KGYEGKTHKG DIKQQAWIQK ISELIKRPNV SPKVRELLEQ

101 ISAFDNVPRK KAKFQNWMKN SLKVHNESIL DQVWNIFSEA SNSEPVNKEQ

151 DQRPLHPVAN PHAEISTKVP ASKVKDAVEQ QGEVKKNKRE RKEERQKKRK

201 REKKELKLEN HQENSRNQKP KKRKKGQEAD LEAGGEEVPE ANGSAGKRSK

251 KKKQRKDSAS EEEARVGAGK RKRRHSEVET DSKKKKMKLP EHPEGGEPED

301 DEAPAKGKFN WKGTIKAILK QAPDNEITIK KLRKKVLAQY YTVTDEHHRS

351 EEELLVIFNK KIΞKNPTFKL LKDKVKLVK

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_14g5, frame 3

PIR:A40683 cell growth regulating nucleolar protein LYAR - mouse, N = 1, Score = 1410, P = 2.7e-144

SWISSPROT :YQ58_CAEEL HYPOTHETICAL 28.5 KD PROTEIN C16C10.8 IN CHROMOSOME III., N = 1, Score = 381, P = 2.9e-35

TREMBL:AC003058_18 gene: "F27F23.18"; product: "putative RNA-bindmg protein"; Arabidopsis thaliana chromosome II BAC F27F23 genomic sequence, complete sequence., N = 3, Score = 139, P = 4e-15

PIR:S70049 nucleic acid-bmding protein YCR087c-a - yeast (Saccharomyces cerevisiae), N = 1, Score = 164, P = 1.4e-ll

>PIR:A40683 cell growth regulating nucleolar protein LYAR - mouse Length = 388

HSPs:

Score = 1410 (211.6 bits), Expect = 2.7e-144, P = 2.7e-144 Identities = 275/388 (70%), Positives = 317/388 (81%)

Query: 1 MVFFTCNACGESVKKIQVEKHVSVCRNCECLSCIDCGKDFWGDDYKNHVKCISEDQKYGG 60

MVFFTCNACGESVKKIQVEK VS CRNCECLSCIDCGKDFWGDDYK+HVKCISE QKYGG Sbjct: 1 MVFFTCNACGESVKKIQVEKQVSNCRNCECLSCIDCGKDFWGDDYKSHVKCISEGQKYGG 60

Query: 61 KGYEGKTHKGDIKQQAWIQKISELIKRPNVSPKVRELLEQISAFDNVPRKKAKFQNWMKN 120

KGYE KTHKGD KQQAWIQKI+ELIK+PNVSPKVRELL+QISAFDNVP KKAKFQNWMKN Sbjct: 61 KGYEAKTHKGDAKQQAWIQKINELIKKPNVSPKVRELLQQISAFDNVPIKKAKFQNWMKN 120

Query: 121 ΞLKVHNESILDQVWNIFSEASNSEPVNKEQDQRPLHPVANPHAEIS-TKVPASKVKDAVE 179

SLKVH++S+L+QVW+IFSEAS+SE ++Q Q P H A PHAE+ TKVP++K E Sbjct: 121 SLKVHSDSVLEQVWDIFSEASSSE QDQQQPPSH-TAKPHAEMPITKVPSAKTNGTTE 176

Query: 180 QQGEVKKNKRERKEERQKKRKREKKELKLENHQENSRNQKPKKRKKGQEADLEAGGEEVP 239

+Q E KKNKRERKEERQK RK+EKKELKLENHQEN R QKPKKRKK QEA EA GE+ Sbjct: 177 EQTEAKKNKRERKEERQKNRKKEKKELKLENHQENLRGQKPKKRKKNQEAGHEAAGEDGA 236

Query: 240 EANG SAGKRSKKKKQRKDSASEEEA RVGAGKRKR-RHSEVETDSKKKKM 287

+ +G G+ S++ R E+ A + AGKRKR +HS E+ KKKKM

Sbjct: 237 DGSGPPEKKKAQGGQASEEGADRNGGPGEDRAEGQTKTAAGKRKRPKHSGAESGYKKKKM 296

Query: 288 KLPEHPEGGEPEDDEAPAKGKFNWKGTIKAILKQAPDNEITIKKLRKKVLAQYYTVTDEH 347

KLPE PE GE +D EAP+KGKFNWKGTIKA+LKQAPDNEI++KKL+KKV+AQY+ V ++ Sbjct: 297 KLPEQPEEGEAKDHEAPSKGKFNWKGTIKAVLKQAPDNEISVKKLKKKVIAQYHAVMNDT 356

Query: 348 HRSEEELLVIFNKKISKNPTFKLLKDKVKLVK 379

EEELL IFN+KIS+NPTFK+LKD+VKL+K Sbjct: 357 SHHEEELLAIFNRKISRNPTFKVLKDRVKLLK 388

Pedant information for DKFZphtes3_14g5, frame 3 Report for DKFZphtes3_14g5 . 3

[ LENGTH ] 379

[MW ] 43634 . 03

[pi ] 9 . 59

[HOMOL] PIR:A40683 cell growth regulating nucleolar protein LYAR - mouse le-122

[FUNCAT] 04.99 other transcription activities [S. cerevisiae, YCR087c-a] 2e-ll

[BLOCKS] BL00603D Thymidine kinase cellular-type proteins

[BLOCKS] BL00530C

[PROSITE] ATP_GTP_A 1

[KW] All_Alpha

[KW] LOW_COMPLEXITY 18.73 %

SEQ MVFFTCNACGESVKKIQVEKHVSVCRNCECLSCIDCGKDFWGDDYKNHVKCISEDQKYGG SEG

PRD ccccccccccccchhhhhhhheeecccccceeeccccccccccccccceeeeeccccccc

SEQ KGYEGKTHKGDIKQQAWIQKISELIKRPNVSPKVRELLEQISAFDNVPRKKAKFQNWMKN SEG

PRD cccccccccchhhhhhhhhhhhhhhhhccchhhhhhhhhhhhcccccchhhhhhhhhhhc

SEQ SLKVHNEΞILDQVWNIFSEASNSEPVNKEQDQRPLHPVANPHAEISTKVPASKVKDAVEQ SEG

PRD cccccchhhhhhhhhhhhhhhcchhhhhhhhcccccccccccccceeecccccchhhhhh

SEQ QGEVKKNKRERKEERQKKRKREKKELKLENHQENSRNQKPKKRKKGQEADLEAGGEEVPE SEG .... xxxxxxxxxxxxxxxxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccchhhhhhchhhhhccccccc

SEQ ANGSAGKRSKKKKQRKDSASEEEARVGAGKRKRRHSEVETDΞKKKKMKLPEHPEGGEPED

SEG ..xxxxxxxxxxxxxxxxxx xxxxxxxxxxx

PRD cccccccchhhhhhhhccchhhhhhhhhcccccccccccccchhhhhhcccccccccccc

SEQ DEAPAKGKFNWKGTIKAILKQAPDNEITIKKLRKKVLAQYYTVTDEHHRSEEELLVIFNK SEG xxxxx

PRD cccccceeeehhhhhhhhhhhccccccchhhhhhhhhhhhhhhccchhhhhhhhhhhhhh

SEQ KISKNPTFKLLKDKVKLVK

SEG xxxxxxxxxxx

PRD ccccccchhhhhhhhhccc

Prosite for DKFZphtes3_14g5.3 PS00017 60->68 ATP GTP A PDOC00017

(No Pfam data available for DKFZphtes3_14g5.3)

DKFZphtes3 14h21

group: nucleic acid management

DKFZphtes3_14h21 encodes a novel 648 amino acid protein with strong similarity to mus musculus RNA helicase and several RNA-dependent ATPases from the DEAD box family.

RNA helicases comprise a large family of proteins that are involved in basic biological systems such as nuclear and mitochondrial splicing processes, RNA editing, rRNA processing, translation initiation, nuclear mRNA export, and mRNA degradation. RNA helicases are essential factors in cell development and differentiation, and some of them play a role in transcription and replication of viral single-stranded RNA genomes. The members of the largest subgroup, the DEAD and DEAH box proteins, exhibit a strong dependence of the unwinding activity on ATP hydrolysis. The novel protein contains a DEAD-box and a ATP/GTP-bindmg site motif A (P-loop) and is a new member of this subgroup.

The new protein can find application in modulating RNA metabolism and gene expression. strong similarity to RNA helicases start at Bp 33 matches Kozak consensus ACNatg

Sequenced by BMFZ

Locus : unknown

Insert length: 2200 bp

Poly A stretch at pos. 2166, polyadenylation signal at pos. 2140

1 CAACGACGTC GGACGCGCCC CTTCTTGGAA CAATGTCCCA CCACGGAGGA 51 GCTCCCAAGG CCTCTACGTG GGTCGTTGCT AGTCGGCGAA GCTCGACAGT

101 GTCCCGAGCG CCAGAGAGGA GGCCGGCGGA GGAGTTGAAT CGAACAGGTC

151 CTGAGGGATA TAGTGTCGGC AGAGGTGGTC GCTGGAGAGG CACCTCTAGG

201 CCCCCGGAGG CCGTGGCCGC TGGTCACGAG GAACTGCCGC TGTGTTTTGC

251 TTTGAAGAGC CACTTTGTTG GCGCGGTAAT CGGTCGTGGT GGGTCAAAAA

301 TAAAGAATAT ACAAAGTACA ACAAACACCA CAATCCAAAT AATACAAGAA

351 CAACCAGAAT CATTAGTCAA AATTTTTGGC AGCAAGGCAA TGCAAACGAA

401 AGCAAAAGCA GTGATAGACA ATTTTGTTAA AAAGCTAGAA GAAAATTACA

451 ATTCAGAATG CGGAATTGAT ACTGCATTCC AACCTTCTGT TGGAAAAGAT

501 GGAAGCACAG ATAACAATGT TGTTGCAGGA GATCGGCCAT TGATAGATTG

551 GGATCAAATT AGAGAGGAAG GTTTGAAATG GCAAAAAACA AAGTGGGCAG

601 ATTTACCACC AATTAAGAAA AACTTTTATA AAGAGTCCAC TGCCACAAGT

651 GCCATGTCAA AAGTAGAAGC AGATAGTTGG AGGAAAGAAA ATTTTAATAT

701 AACGTGGGAT GACTTGAAGG ATGGGGAGAA ACGACCTATC CCCAATCCTA

751 CCTGCACATT TGATGACGCC TTTCAATGTT ATCCTGAGGT TATGGAAAAC

801 ATTAAAAAGG CAGGTTTTCA AAAGCCAACA CCTATTCAGT CACAGGCATG

851 GCCCATTGTG TTGCAAGGAA TAGATCTTAT AGGAGTAGCC CAGACTGGAA

901 CAGGAAAGAC ATTGTGTTAT TTAATGCCTG GATTTATTCA TCTGGTCCTT

951 CAACCCAGCC TTAAAGGTCA AAGGAATAGA CCCGGCATGT TAGTTCTAAC 1001 TCCCACTCGG GAATTAGCAC TTCAAGTAGA AGGAGAATGT TGCAAATATT 1051 CATATAAAGG GCTTCGGAGT GTTTGTGTAT ATGGTGGTGG AAATAGAGAT 1101 GAACAAATAG AAGAGCTTAA AAAAGGTGTA GATATCATAA TTGCAACTCC 1151 CGGAAGATTG AATGATCTGC AAATGAGTAA CTTCGTCAAT CTGAAGAATA 1201 TAACCTACTT GGTTTTAGAT GAAGCAGACA AGATGTTGGA CATGGGATTT 1251 GAACCCCAGA TAATGAAGAT TTTGTTAGAT GTGCGCCCAG ATAGGCAGAC 1301 AGTTATGACC AGTGCTACAT GGCCTCATTC AGTTCATCGC CTCGCACAAT 1351 CTTATTTGAA AGAACCAATG ATTGTCTATG TTGGTACATT GGATCTAGTT 1401 GCTGTAAGTT CAGTGAAGCA AAATATAATT GTAACCACCG AGGAAGAGAA 1451 ATGGAGTCAC ATGCAAACTT TTCTACAGAG TATGTCATCC ACAGACAAAG 1501 TCATTGTCTT CGTTTCTCGA AAAGCTGTTG CGGATCACTT ATCAAGTGAC 1551 CTAATACTTG GAAATATATC AGTAGAGTCT CTGCATGGAG ATAGAGAACA 1601 GAGAGATCGG GAGAAAGCAT TAGAGAACTT TAAAACAGGC AAAGTGAGAA 1651 TACTAATTGC AACTGATCTA GCCTCTAGAG GACTTGATGT CCATGACGTT 1701 ACACATGTCT ATAATTTTGA CTTTCCACGG AATATTGAAG AATACGTACA 1751 CCGAATAGGG CGCACGGGAA GAGCAGGGAG GACTGGTGTT TCCATTACAA 1801 CTTTGACTAG AAATGATTGG AGGGTTGCCT CTGAATTGAT TAATATTCTG 1851 GAAAGAGCAA ATCAGAGTAT TCCAGAGGAG CTTGTATCAA TGGCTGAGAG 1901 GTTTGAGGCA CATCAACGGA AAAGGGAAAT GGAAAGAAAA ATGGAAAGAC 1951 CTCAAGGAAG GCCCAAGAAG TTTCATTAAT GTCTTCTGTA CTAGTGGGGT 2001 AGAGAATTCA AGATTTTTTA GAAATATAGT AAGACAGAAG TATTGGACAT 2051 GTTGGCAGTA TGAAGAGACC GGACTGATTT GACTGATTCT TAAAATAATA 2101 GTGTTTGAAA ATATAGAATC CAGTGTTTTA TACTTTCTTT AATAAAAATA 2151 GAAGTATTTA AACTTGAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

BLAST Results No BLAST result

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 33 bp to 1976 bp; peptide length: 64£ Category: strong similarity to known protein Classification: Nucleic acid management Prosite motifs: ATP_GTP_A (286-294) DEAD ATP HELICASE (394-403)

1 MSHHGGAPKA STWVVASRRS STVSRAPERR PAEELNRTGP EGYSVGRGGR

51 WRGTSRPPEA VAAGHEELPL CFALKSHFVG AVIGRGGSKI KNIQSTTNTT

101 IQIIQEQPES LVKIFGSKAM QTKAKAVIDN FVKKLEENYN SECGIDTAFQ

151 PSVGKDGSTD NNVVAGDRPL IDWDQIREEG LKWQKTKWAD LPPIKKNFYK

201 ESTATSAMSK VEADSWRKEN FNITWDDLKD GEKRPIPNPT CTFDDAFQCY

251 PEVMENIKKA GFQKPTPIQS QAWPIVLQGI DLIGVAQTGT GKTLCYLMPG

301 FIHLVLQPSL KGQRNRPGML VLTPTRELAL QVEGECCKYS YKGLRΞVCVY

351 GGGNRDEQIE ELKKGVDIII ATPGRLNDLQ MSNFVNLKNI TYLVLDEADK

401 MLDMGFEPQI MKILLDVRPD RQTVMTSATW PHSVHRLAQS YLKEPMIVYV

451 GTLDLVAVSS VKQNIIVTTE EEKWSHMQTF LQSMSSTDKV IVFVSRKAVA

501 DHLSSDLILG NISVESLHGD REQRDREKAL ENFKTGKVRI LIATDLASRG

551 LDVHDVTHVY NFDFPRNIEE YVHRIGRTGR AGRTGVSITT LTRNDWRVAS

601 ELINILERAN QSIPEELVSM AERFEAHQRK REMERKMERP QGRPKKFH

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_14h21, frame 3

TREMBL :CEY54G11A_9 gene: "Y54G11A.3"; Caenorhabditis elegans cosmid Y54G11A, N = 1, Score = 1008, P = 1. le-101

TREMBL :SPBP8B7_16 gene: "dbp2"; "SPBP8B7.16c"; product: "p68-lιke protein."; S. pombe chromosome II pi p8B7., N = 1, Score = 971, P = 9.1e-98

PIR:S13757 RNA helicase DBP2 - yeast (Saccharomyces cerevisiae), N = 1, Score = 970, P = 1.2e-97

PIR:S14048 RNA helicase dbp2 - fission yeast (Schizosaccharomyces pombe), N = 1, Score = 961, P = le-96

PIR:A57514 RNA helicase HEL117 - rat, N = 2, Score = 888, P = 7.8e-91

>TREMBL:CEY54G11A_9 gene: "Y54G11A.3"; Caenorhabditis elegans cosmid Y54G11A

Length = 504

HSPs:

Score = 1008 (151.2 bits), Expect = 1. le-101, P = 1. le-101 Identities = 211/473 (44%), Positives = 298/473 (63%)

Query: 174 DQIREEGLKWQKTKWADLPPIKKNFYKESTATSAMSKVEADSWRKENFNITWDDLKDGEK 233

D++++E W K PI ++ YK +S + + ++

Sbjct: 23 DRLKDENFSWMK PIVRDLYKIPNEQKNLSPEQLQELYTNGGVMKVYPFREEST 75

Query: 234 RPIPNPTCTFDDAFQCYPEVMENIKKAGFQKPTPIQSQAWPIVLQGIDLIGVAQTGTGKT 293

IP P +F+ AF +M I+K GF+KP+PIQSQ WP++L G D IGV+QTG+GKT Sbjct: 76 VKIPPPVNSFEQAFGSNASIMGEIRKNGFEKPSPIQSQMWPLLLSGQDCIGVSQTGSGKT 135

Query: 294 LCYLMPGFIHLVLQPSL KGQRNRPGMLVLTPTRELALQVEGECCKYSYKGLRΞVC 348

L +L+P +H+ Q + + Q+ P _+LVL+PTRELA Q+EGE KYSY G +ΞVC Sbjct: 136 LAFLLPALLHIDAQLAQYEKNDEEQKPSPFVLVLSPTRELAQQIEGEVKKYSYNGYKSVC 195

Query: 349 VYGGGNRDEQIEELKKGVDIIIATPGRLNDLQMSNFVNLKNITYLVLDEADKMLDMGFEP 408 +YGGG+R EQ+E + GV+I+IATPGRL DL ++L ++TY+VLDEAD+MLDMGFE Sbjct: 196 LYGGGSRPEQVEACRGGVEIVIATPGRLTDLSNDGVISLASVTYVVLDEADRMLDMGFEV 255

Query: 409 QIMKILLDVRPDRQTVMTSATWPHSVHRLAQSYLKEPMIVYVGTLDLVAVSSVKQNIIVT 468

I +IL ++RPDR +TSATWP V +L Y KE ++ G+LDL + SV Q Sbjct: 256 AIRRILFEIRPDRLVALTSATWPEGVRKLTDKYTKEAVMAVNGSLDLTSCKSVTQFFEFV 315

Query: 469 TEEEKW SHMQTFLQSMSSTD-KVIVFVSRKAVADHLSSDLILGNISVESLHGDREQR 524

+ ++ + FL + + K+I+FV K +ADHLSSD + 1+ + LHG R Q Sbjct: 316 PHDSRFLRVCEIVNFLTAAHGQNYKMIIFVKSKVMADHLSSDFCMKGINSQGLHGGRSQS 375

Query: 525 DREKALENFKTGKVRILIATDLASRGLDVHDVTHVYNFDFPRNIEEYVHRIGRTGRAGRT 584

DRE +L ++G+V+IL+ATDLASRG+DV D+THV N+DFP +IEEYVHR+GRTGRAGR Sbjct: 376 DREMSLNMLRSGEVQILVATDLASRGIDVPDITHVLNYDFPMDIEEYVHRVGRTGRAGRK 435

Query: 585 GVSITTLTRNDWRVASELINILERANQSIPEELVSMAERFEAHQRKREMERKMERPQGRP 644

G +++ L ND LI ILE++ Q +P++L AE++ K + R RP R Sbjct: 436 GEAMSFLWWNDRSNFEGLIQILEKSEQEVPDQLRRDAEKYRL KCQSGRDGPRPSFRN 492

Query: 645 KK 646

K Sbjct: 493 NK 494

Pedant information for DKFZphtes3_14h21, frame 3

Report for DKFZphtes3_14h21.3

[LENGTH] 648

[MW] 72873.51

[pi] 8.84

[HOMOL] TREMBL :CEY54G11A_9 gene: "Y54G11A.3"; Caenorhabditis elegans cosmid Y54G11A le-

101

[FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YNL112w] 2e-97

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YNL112w] 2e-97

[FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YPL119C] 4e-72

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YOR204w] 2e-70

[FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae,

YOR204w] 2e- 70

[ FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YBR237w] le-61

[FUNCAT] 1 genome replication, transcription, recombination and repair [H. influenzae, HI0892] 2e-49

[FUNCAT] j mrna translation and ribosome biogenesis [H. influenzae, HI0231 RNA] le- 48

[FUNCAT] 04.99 other transcription activities [S. cerevisiae, YDL160C] 9e- -45

[FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YMR290C] 3e- -44

[FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YJL033w] 2e- -36

[FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YOR046C] 7e- -32

[FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YDR194C] 2e- -28

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YGL064c] 5e-10

[FUNCAT] 11.10 cell death [S. cerevisiae, YMR190c] 2e-08

[FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YMR190c] 2e-08

[FUNCAT] r general function prediction [M. jannaschn, MJ1401] le-07

[BLOCKS] BL00039D DEAD-box subfamily ATP-dependent helicases proteins

(BLOCKS] BL00039C DEAD-box subfamily ATP-dependent helicases proteins

[BLOCKS] BL00039B DEAD-box subfamily ATP-dependent helicases proteins

[BLOCKS] BL00039A DEAD-box subfamily ATP-dependent helicases proteins

[PIRKW] nucleus 4e-96

[PIRKW] RNA binding 3e-87

[PIRKW] DEAD box 5e-50

[PIRKW] transmembrane protein 4e-27

[PIRKW] DNA binding 3e-67

[PIRKW] recF recombination pathway 3e-10

[PIRKW] ATP 4e-96

[PIRKW] purine nucleotide binding 5e-50

[PIRKW] P-loop 4e-96

[PIRKW] hydrolase 9e-45

[PIRKW] protein biosynthesis 5e-50

[PIRKW] ATP binding le-61

[SUPFAM] WW repeat homology 8e-88

[SUPFAM] DEAD/H box helicase homology 4e-96

[SUPFAM] unassigned DEAD/H box helicases 7e-87

[SUPFAM] ATP-dependent RNA helicase DBP1 4e-96

[SUPFAM] ATP-dependent RNA helicase DHH1 2e-43

[SUPFAM] recQ protein 3e-10

[SUPFAM] Bloom's syndrome helicase 5e-07

[SUPFAM] translation initiation factor eIF-4A 5e-50

[SUPFAM] recQ helicase homology 3e-10

[SUPFAM] tobacco ATP-dependent RNA helicase DB10 8e-88

[PROSITE] DEAD ATP HELICASE 1 [PROSITE] ATP_GTP_A 1

[PFAM] Helicases conserved C-terminal domain

[PFAM] KH domain family of RNA binding proteins

[PFAM] DEAD and DEAH box helicases

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 8.49 %

SEQ MSHHGGAPKASTWVVASRRSSTVSRAPERRPAEELNRTGPEGYSVGRGGRWRGTSRPPEA

SEG xxxxxxxxxxxxxxxxx

PRD cccccccccceeeeeecccccccccccccccccccccccccccccccccccccccccccc

SEQ VAAGHEELPLCFALKSHFVGAVIGRGGSKIKNIQSTTNTTIQIIQEQPESLVKIFGSKAM

SEG xxxxxxxxxxxxxxx

PRD cccccccccchhhhhcccceeeecccccccccccccccceeeeecccccceeeeeccchh

SEQ QTKAKAVIDNFVKKLEENYNSECGIDTAFQPSVGKDGSTDNNVVAGDRPLIDWDQIREEG

SEG

PRD hhhhhhhhhhhhhhhhhhhccccccccccccccccccccccccccccccccccccccccc

SEQ LKWQKTKWADLPPIKKNFYKESTATSAMSKVEADSWRKENFNITWDDLKDGEKRPIPNPT

SEG

PRD chhhhhhhcccccccccccccccccchhhhhhhhhhhhhhheeeeecccccccccccccc

SEQ CTFDDAFQCYPEVMENIKKAGFQKPTPIQSQAWPIVLQGIDLIGVAQTGTGKTLCYLMPG

SEG

PRD ccccccccccchhhhhhhhhhcccccccccccccccccccceeeeeecccccceeeecce

SEQ FIHLVLQPSLKGQRNRPGMLVLTPTRELALQVEGECCKYSYKGLRSVCVYGGGNRDEQIE

SEG

PRD eeeeccccccccccccceeeeeccchhhhhhhhhhhhhhhccceeeeeeccccccchhhh

SEQ ELKKGVDIIIATPGRLNDLQMSNFVNLKNITYLVLDEADKMLDMGFEPQIMKILLDVRPD

SEG

PRD hhhhceeeeeeccccchhhhhhhccccccceeeehhhhhhhhhcccchhhhhhhhhhccc

SEQ RQTVMTSATWPHSVHRLAQSYLKEPMIVYVGTLDLVAVSSVKQNIIVTTEEEKWSHMQTF

SEG

PRD ceeeeeecccchhhhhhhhhhhhheeeeeecccccccccccceeehhhhhchhhhhhhhh

SEQ LQSMSSTDKVIVFVSRKAVADHLSSDLILGNISVESLHGDREQRDREKALENFKTGKVRI

SEG

PRD hhhhcccceeeeeeehhhhhhhhhhhhhhcccceeecccccchhhhhhhhhhhhccccee

SEQ LIATDLASRGLDVHDVTHVYNFDFPRNIEEYVHRIGRTGRAGRTGVSITTLTRNDWRVAS

SEG xxxxxxxxxxxx

PRD eeehhhhhhcccccceeeeeeeccccccccceeeecccccccccceeeeeeccccchhhh

SEQ ELINILERANQΞIPEELVSMAERFEAHQRKREMERKMERPQGRPKKFH

SEG xxxxxxxxxxx

PRD hhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhccccccccccc

Prosite for DKFZphtes3_14h21.3

PS00017 286->294 ATP_GTP_A PDOC00017

PS00039 394->403 DEAD ATP HELICASE PDOC00039

Pfam for DKFZphtes3_14h21.3

HMM_NAME DEAD and DEAH box helicases

HMM *gLpPWILRnIyeMGFEkPTPIQQqAIPιILeGRDVMACAQTGΞGKTAAF

P++++NI+++GF KPTPIQ+QA+PI+L+G D+++ AQTG+GKT+++ Query 248 QCYPEVMENIKKAGFQKPTPIQSQAWPIVLQGIDLIGVAQTGTGKTLCY 296

HMM HPMLQHIDwdPWpqpPQd.. PrALILAPTRELAMQIQEEcRkFgkHMng

L+P ++H+ +P +++ Q+ P +L+L+PTRELA+Q++ EC K+++ + Query 297 LMPGFIHLVLQP-SLKGQRNRPGMLVLTPTRELALQVEGECCKYSYK-G- 343

HMM IRImcIYGGtnMRdQMRmLeRGpPHIVIATPGRLIDHIERgtldLDrleM

+R++C+YGG N ++Q+++L++G+ +I+IATPGRL D+ +++ ++L++I++ Query 344 LRSVCVYGGGNRDEQIEELKKGV-DIIIATPGRLNDLQMSNFVNLKNITY 392

HMM LVMDEADRMLDMGFIDQIRrlMrqIPMpwNRQTMMFSATMPdelqELARr

LV+DEAD+MLDMGF++QI++I+ ++ ++RQT+M SAT+P ++ +LA Query 393 LVLDEADKMLDMGFEPQIMKILLDVR—PDRQTVMTSATWPHSVHRLAQS 440 HMM FMRNPIRInld.MdElTtnEnlkQwYiyVerEMWKfdcLcrLIe*

++++P + ++ D +++ +KQ +1+ E++K + ++++ Query 441 YLKEPMIVYVGTLDLVAVS-SVKQNIIVTT-EEEKWSHMQTFLQ 482

HMM_NAME KH domain family of RNA binding proteins

HMM *rIιIPedhMGMIIGKGGsNIRqIREEYgvrINIPdecCeDstdRIITIt

+ + ++++G++IG+GGΞ I++I++ ++++I I++E+ + + + I Query 71 CFALKSHFVGAVIGRGGSKIKNIQΞTTNTTIQIIQEQ-P ESLVKIF 115

HMM G*

G Query 116 G 116

HMM_NAME Helicases conserved C-termmal domain

HMM *EιleeWLknl....GIrvmYIHGdMpQeERdelMddFNnGEynVLIcTD

+ +++ L+ + +I+V ++HGD++Q++R++++++F++G+ ++LI+TD Query 497 KAVADHLSSDLILGNISVESLHGDREQRDREKALENFKTGKVRILIATD 545

HMM VggRGIDIPdVNHVINYDMPWNPEqYIQRIGRTgRIG*

+++RG+D+ DV HV+N+D+P+N+E Y++RIGRTGR+G Query 546 LASRGLDVHDVTHVYNFDFPRNIEEYVHRIGRTGRAG 582

DKFZphtes3_14pl4

group: testes derived

DKFZphtes3_14pl4 encodes a novel 159 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, few EST hits

Sequenced by BMFZ

Locus : unknown

Insert length: 3969 bp

Poly A stretch at pos. 3948, polyadenylation signal at pos. 3927

1 GAAGCCCAGG CTCTCCTTAG TTGACTGTGT GTTAATCACC CAGCAATTTC 51 ATTACTCAAC AGCTCTCCAG AGTTGCACAT TACAGCTGGG GTAGAAATTG

101 GGTGCTGAAG GCCAGGCAGA GCATTTGGCT GTAGGGAGGC CGATCCTCCT

151 CGGGCCTGTT ACCGGCGGGT CTTTGTTCTT AGACCTGGGG TTCTTGGCCT

201 CACGGATTCC AAGGAATGGA ACGTTGGGCC ATGCGTGTGA ACGAGCTCTA

251 TGTCGATGAC CCAGACAAGG ACAGCGGTGG CAAGATCGAC GTCAGTCTGA

301 ACATCAGTTT ACCCAATCTG CACTGCGAGT TGGTTGGGCT TGACATTCAG

351 GATGAGATGG GCAGGCACGA AGTGGGCCAC ATCGACAACT CCATGAAGAT

401 CCCGCTGAAC AATGGGGCAG GCTGCCGCTT CGAGGGGCAG TTCAGCATCA

451 ACAAGGTATG GAAGCCCTGC CTCAGCCCTT TCTACCTGCT CCCCTTTCCT

501 GCTGTCTCCC CGCTCCCTGG AAACTGGTTG TGGAGGCACT CACTCGACCT

551 GACCCTGACA CAGCCCCCAG CAAGCGAGGG TTCGTGTCCA GCTGCCTGGC

601 CGTTCCTGCT GAGAATCTGG ATGGGGGTCC AGGCTCCCTG GGGTTTTAAG

651 CCCCTGATGG CTGGTTCAGG AAGGAGCTAC TCTTCTCTCC AGTGAGGGGG

701 ACAATGATGA GAAGACCTGA GGATTTGCAG CCCCCAGCCC TGGGTTCAAG

751 TCCCAGCTCT ACCCCTTCTT GGCCCCTACA AGTCACTTGA CCCATCTTAG

801 GCTGAGGGTG TGATGGCGAT AATAGTATCA CGATACCACC CACTTCACAA

851 AGTTTGTGTG GGGATTAAAT GAGCTAATGC AGATTCATTC ATTCAGAAAA

901 ATTTTTGAAT GGCACGTTCT GTGTTCCAGG GTCGGTGATA GGCTCTGGGG

951 CAGCGTTCCT GGGCTGGTGG GGCTCCCATT CTGGTAGAGG GAGACAGTCT 1001 ACAAACCAGA AAGCATCAGG GATGCTAAGT GCAGTGATGA GGAATAAAGC 1051 CAAGGGGAGT GAGATGAGGT GGGCTTGAAA GTACCTTGTC CGCTCAGAAG 1101 GACCATTCAA GGTTCACTGT TGTTTTGTCC TCAGAACCAG GAGCTTCAGA 1151 TCCTAAGTCA AGTGGGTGAA CGCAGTGCCC TTGGGAGGGC CGAGGCACCC 1201 GGTGGCAGCT GGCAGGGTTT TGCTCAGCAC GTGCCGGCCT TCCTCGAAGC 1251 TCGGTACTGT CACAGTGGAG CCTCTCAACA ACGCTGTGAG GCAGCACCAT 1301 TTGACAGGTT AGGATGCTGG GGCCCAGAGA GGTTAAGTGT CTTGCCCGAG 1351 GTCACACAGC TATCTGCATG TCCCACAACT CCCCTTCCCA GCCCCAGCCA 1401 AACTGAGCCA CTGGCCACTC CTGGCTTCTC CTTGTCCCTC CTGCAGCCTC 1451 TGCTCAGAAC GCCCTTCCTC CAGACCCTGA CACCTGAGCT GGGGTTGCAA 1501 AGTCACTGGC CACATCCAGC CCAAAGATAA ATTTTGTTTG TCCAGTATAG 1551 CATTTAACTG CATCAGAACC AGTATGAAAA GACCAGGAAT CCAGATTTCT 1601 GGCTTTTAAA AGTCAGAGGC TCTCACTACA CTGGGTCCGT GTTCCCGCTA 1651 TGACAATGAC CTGGCACCAA TGGGCAGTGT TCCCCTTTAG AGAGGGTGTG 1701 TGCTGTCCCT TCCCACAGTC CCTGGCAGGC GGCTGGAAGG CCAGGCCTGG 1751 TCATCTGTCA AGCAGGGTGG ACTTCTTACG TGACAGTTCA GGGCTCCCTT 1801 AAGTGCTAAA GCAGAAGCTG CAAGGCTTTC TTAAGGTTTC GAGTGTTGCT 1851 GGGAGAAATC TGCTGCATGT TGTGGGTTAA AGGGAGTCTC TCACCAGCCC 1901 AGGCCCTCAG. GAGGAGGAGA TACCAGGAGG CAGGGATGCT GGGGGTCGTG 1951 GTTCACTGGG^* GGCTCTCTCT GCCCATGAGC TGCCACACAG CACCTTTGCC 2001 ATGCCCCGTA ATTTGGATTT TATGGTGGTT GTGATGGAAA GCCATTTGAG 2051 GGTTTTGAAC AGGGAGGCAA TGTAATCAGA TTTATGCCTT AGAACTGGAC 2101 TATCCAATAG GTTGCCACCA GCCACATAAG GCTATTTAAA TTAATTCAAA 2151 TTAAATGTAC AATTCAGTCA CTCATTCTCA TCAACCACAT TTCAAGTGCT 2201 CAAAGCCACG TGCTGGCTAG GGGCCACAGC GTTAGACAGT GCAGAGAGAA 2251 AGCACTTCCA TCGCTGAGGA AAGTTCTGCT GGACCGCACA CCCTTAGAAG 2301 GATGGCTCTG GTGGCCGGGC GCGGTGGCTC AAACCTGTAA TCCCAGCACT 2351 TTGGGAGGCC GAGGTGGGTG GATCACGAGG TCAGGAGATC GAGACCATCC 2401 CGGCTAACAT GGTGAAACCC TGCCTCTACT AAAAATACAA AAAAAAACAA 2451 AATTAGCCGG GCGTGGTTGC GGGCACCTGT AGTCCCAGCT ACTCAGGAGG 2501 CTGAGGCGGG AGAATGGCAT GAACCCGGGA GGTGGAGCTT GCAGTGAGCC 2551 AAGATCGTAC CACTGCACTC CAGTCTGGGC GACAGAGTGA GACTCCATCT 2601 CAAAACAAAC AAAAAAAGGA TGGGGCTGGG CTGGAGAGGG TGGCAGGCAG 2651 TGGTTGTGGC AGTGGAGCTG GGGAGATGTG GTCGGATTAG GGAGGTAGAA 2701 TCAATAAGAC TCAGTGAAGA ATCGGATGTG GGGGTAAGGG CACATGTGGA 2751 AGCAAAGAAA CCTTTGACGT CTTTGTCTTG ACAACCGGGT GGTCCTGTTT 2801 CTAGACATGG AAGCTTAGAA AAGCCTGGAG TCTGTGGGAA GTAGGTAGGG 2851 CTGGGCACTG GTCATTCCAC TCTGGTTTCC TTTGGGGTTC CCATTAGGTG 2901 TCTACAGGGA GAGGTGAAAT TGGAAGTTGG AGGTGTGGAG AGTTCAGGAG

2951 AGGGTTCTGG ACCACAGATG TTGAGGTGGG AGTCATTAGT GAATAGATGA 3001 TGTTGGAAGT CATGGGTCCT CAGAGTGGGG GCTCCTTAAG CCTCCAGGCC 3051 AGCAGCATCA GCATCACCTG GGAGATTGTT AGGAATGCAG ATTCTCAGGC 3101 CCCCCTAAGA CCCACCGACT CTGTGCTAGA ACAAGCGCCC CTCAGAGATT 3151 CTGATGCCAC TGAAGTTTGA GGAGCATTGG TTTAAGCAAG ATTACCTACG 3201 GAGAGGCTGT AGATCCGTGT TCTAAACCTG GGGTCCACAG ACACCCCCAA 3251 GAAGAGCGGA TTGAATGCAA GAGATCTATG AAGTTGGATG GGGGAAAAAT 3301 TGACATCTTT ATTTTTGCTA AACTCGATCT AAAGTTTAGC ATTTCCATCT 3351 GCGATGAATG TAGGCCACAA ACCACAGTAG TATTAGCAGT GCCTGGGACC 3401 TCCTCAACAA CAGAAATTGC CGGTATTTAT AGCACGTTAC AGTTGTTGCA 3451 GATAATTTCC AGAGACTGTT TATATGCACC ACTGTTTTAA AATTACGGTG 3501 ATTGGCCAGG TGCAGTGGCT CACACCTGTA ATCCCAGCAC TTTGGGAGGC 3551 CAAAGTGGGT GGATCACTTG AGGAGTTCAA GACCAGCCTG GTCAACATGT

3601 CAAAACCCTG TATCTACAAA AAAATACAAA AGTTAACCAA GCCTATGCTT 3651 GTAGTCACAG CTACTCGGGA GGCCGAGGTG GGAGGGTCTT CTGAGCCCAG 3701 GGAGGTAGAG GCTTCAGTGA GCTGAGATCG CACCACCACA CTCCAGCCTG 3751 GGTGACAGAG TGAAACCCTT AATCAATCAG TCAATAAAAA TTACAGTAAT 3801 TATTAGACCC ACCACTAGGT CATCTTATTT GATGCATCAG TAAAGCAGCA 3851 TATTCAAATG TGGATTTTTA AATATTTTAA TTACTATTTA AATATCTCTT 3901 TACTTTGTAA TCCTATGCAT TTTACGCATT AAAACATTTT AAGCATTTAA 3951 AAAAAAAAAA AAAAAAAAA

BLAST Results

No BLAST result

Medlme entries

No Medlme entry

Peptide information for frame 3

ORF from 216 bp to 692 bp; peptide length: 159 Category: putative protein Classification: no clue

1 MERWAMRVNE LYVDDPDKDS GGKIDVSLNI SLPNLHCELV GLDIQDEMGR

51 HEVGHIDNSM KIPLNNGAGC RFEGQFSINK VWKPCLSPFY LLPFPAVSPL

101 PGNWLWRHSL DLTLTQPPAS EGSCPAAWPF LLRIWMGVQA PWGFKPLMAG 151 ΞGRSYSSLQ

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_14pl4, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphtes3_14pl4, frame 3

Report for DKFZphtes3_14pl4.3

[LENGTH] 159

[MW] 17778.55

[pi] 5.74

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YAL042w] 5e-04

[KW] Alpha_Beta

SEQ MERWAMRVNELYVDDPDKDSGGKIDVSLNISLPNLHCELVGLDIQDEMGRHEVGHIDNSM PRD ccchhhhhhhhccccccccccceeeeeeccccccccceeeehhhhhhcccceeecccccc

SEQ KIPLNNGAGCRFEGQFSINKVWKPCLSPFYLLPFPAVSPLPGNWLWRHSLDLTLTQPPAS PRD eeecccccceeecccccccccccccccccccccccccccccccccccccccccccccccc SEQ EGSCPAAWPFLLRIWMGVQAPWGFKPLMAGSGRSYSSLQ PRD ccccccchhhhhhhhhhhccccccccccccccccccccc

(No Prosite data available for DKFZphtes3_14pl4.3) (No Pfam data available for DKFZphtes3_14pl4.3)

DKFZphtes3_14p7

group: testes derived

DKFZphtes3_14p7 encodes a novel 702 ammo acid protein with very weak similarity to kinesm associated protein KAP3.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes. weak similarity to kinesin associated protein KAP3 complete cDNA, complete eds, few EST hits

Sequenced by BMFZ

Locus: unknown

Insert length: 2497 bp

Poly A stretch at pos. 2424, polyadenylation signal at pos. 2400

1 GGAATCCAAA GAAACAGTTA TGATGGGGGA CTCTATGGTG AAAATAAATG

51 GGATTTATTT AACAAAATCA AATGCTATTT GCCACTTAAA GAGTCACCCA

101 CTTCAGCTAA CTGATGATGG AGGCTTCAGT GAAATAAAGG AGCAAGAAAT

151 GTTCAAAGGA ACAACATCTT TACCATCTCA TCTCAAGAAT GGAGGGGACC

201 AGGGGAAGAG ACATGCGAGG GCCTCATCAT GCCCCAGTAG CTCAGACCTG

251 AGCAGGCTGC AAACCAAAGC AGTCCCAAAA GCTGACCTGC AAGAAGAGGA

301 CGCAGAAATA GAAGTAGACG AAGTCTTTTG GAATACAAGG ATTGTACCGA

351 TTTTGCGTGA ATTAGAAAAG GAAGAAAACA TTGAAACGGT TTGTGCTGCT

401 TGCACACAAC TTCATCATGC TTTAGAGGAA GGAAACATGC TTGGAAATAA

451 ATTTAAGGGA AGAAGTATTC TCCTGAAGAC CCTGTGTAAA CTAGTTGATG

501 TTGGTTCAGA CTCGCTCAGC CTTAAACTTG CAAAAATAAT TCTAGCACTT

551 AAAGTGAGTA GAAAGAATCT TCTTAATGTC TGCAAACTTA TATTTAAAAT

601 TAGCAGGAAT GAGAAGAATG ATTCTTTGAT TCAAAATGAC AGCATTCTGG

651 AATCATTATT GGAGGTACTA AGAAGTGAAG ACCTGCAAAC TAACATGGAA

701 GCTTTTTTAT ACTGTATGGG GTCTATAAAG TTCATTTCTG GAAATCTGGG

751 ATTTCTTAAT GAAATGATCA GCAAAGGTGC TGTGGAAATA CTGATAAATT

801 TGATAAAACA AATAAATGAG AACATCAAGA AATGTGGTAC ATTTTTGCCT

851 AATTCGGGCC ACTTGCTAGT CCAGGTGACT GCTACATTGA GAAACTTGGT

901 TGATTCATCA TTAGTAAGAA GTAAGTTCCT AAACATCAGT GCCCTTCCCC

951 AGCTCTGCAC GGCAATGGAA CAGTACAAGG GTGACAAGGA CGTCTGTACC

1001 AATATTGCCA GAATATTCAG CAAACTTACT TCTTACCGTG ACTGCTGCAC

1051 AGCCTTGGCC AGCTATTCCA GATGTTATGC CTTATTTCTG AATCTAATTA

1101 ACAAATACCA GAAGAAGCAG GATTTAGTCG TCCGTGTTGT TTTTATTCTT

1151 GGCAACCTGA CGGCAAAAAA TAACCAGGCT CGTGAACAAT TTTCCAAAGA

1201 GAAAGGGAGC ATCCAAACTC TGCTGTCATT ATTCCAGACG TTCCATCAGC

1251 TGGATCTGCA TTCCCAGAAG CCGGTGGGCC AACGAGGCGA GCAGCACAGG

1301 GCGCAGAGGC CGCCGTCAGA GGCAGAGGAC GTGCTCATCA AGCTGACTCG

1351 TGTGCTGGCC AACATTGCCA TCCACCCGGG CGTGGGCCCG GTGCTGGCCG

1401 CCAACCCGGG GATAGTGGGC CTGCTCCTGA CCACGCTGGA ATACAAGTCA

1451 CTTGATGATT GTGAGGAGCT GGTGATCAAT GCTACAGCGA CAATCAACAA

1501 TTTATCTTAC TACCAAGTGA AGAATTCCAT AATTCAAGAC AAAAAGCTAT

1551 ATATTGCTGA ATTGCTCTTA AAGCTTCTTG TCAGTAACAA CATGGATGGA

1601 ATCCTGGAGG CTGTGCGTGT TTTCGGAAAT CTCTCCCAGG ACCATGATGT

1651 CTGCGATTTC ATTGTGCAGA ACAATGTCCA CAGGTTCATG ATGGCGCTGC

1701 TGGATGCTCA GCATCAGGAT ATCTGCTTTT CTGCCTGTGG TGTTCTCCTC

1751 AATCTCACTG TGGATAAAGA CAAGCGTGTC ATCTTGAAAG AAGGAGGTGG

1801 CATTAAAAAG TTAGTGGACT GTTTAAGAGA TTTGGGTCCT ACTGATTGGC

1851 AGCTGGCCTG CTTGGTTTGT AAAACTTTAT GGAACTTCAG TGAAAACATC

1901 ACTAATGCTT CGTCATGTTT TGGAAATGAA GACACCAACA CACTCTTACT

1951 CTTGCTCTCA TCATTTTTAG ATGAAGAACT AGCACTGGAT GGCAGTTTTG

2001 ATCCAGACCT AAAAAACTAT CACAAACTCC ATTGGGAAAC AGAATTCAAA

2051 CCTGTGGCAC AGCAGCTTCT AAACCGAATT CAGAGACATC ACACCTTCCT

2101 GGAACCCCTG CCCATTCCCT CTTTCTAACA TGATGCAGAT TAACAGTAGA

2151 AACGAGAACT CACGTCTCCC TCATTCTTAA GAACTGGTAA CAAACGTGAA

2201 CATTTTTTTC AGCATTAACA AATGTGGAAA GTTTTTCAAG AACTGGTTTT

2251 AGTGAGTAGC TGAAGTATTT TTTAAAATTA AGCATTTCTT CTTGTTAGGT

2301 ATTATGGAAA AATGAATATA CACATTATAT TTCCTGTTGA GAGAAATGTA

2351 AGATGAAAAT ATGTGCATTT TCAAGTAAAT GACTTTTTCT TCTATTCTCT

2401 ATTAAACAAT TTAGTTCTAG TCTTAAAAAA AAAAAAAAAA AAAAAAAAAA

2451 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAA

BLAST Results No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 20 bp to 2125 bp; peptide length: 702 Category: putative protein

1 MMGDSMVKIN GIYLTKSNAI CHLKSHPLQL TDDGGFSEIK EQEMFKGTTS 51 LPSHLKNGGD QGKRHARASS CPSSSDLSRL QTKAVPKADL QEEDAEIEVD 101 EVFWNTRIVP ILRELEKEEN IETVCAACTQ LHHALEEGNM LGNKFKGRSI 151 LLKTLCKLVD VGSDSLSLKL AKIILALKVS RKNLLNVCKL IFKISRNEKN 201 DSLIQNDΞIL ESLLEVLRSE DLQTNMEAFL YCMGSIKFIS GNLGFLNEMI 251 SKGAVEILIN LIKQINENIK KCGTFLPNSG HLLVQVTATL RNLVDSSLVR 301 SKFLNISALP QLCTAMEQYK GDKDVCTNIA RIFSKLTSYR DCCTALASYS 351 RCYALFLNLI NKYQKKQDLV VRVVFILGNL TAKNNQAREQ FSKEKGSIQT 401 LLSLFQTFHQ LDLHSQKPVG QRGEQHRAQR PPSEAEDVLI KLTRVLANIA 451 IHPGVGPVLA ANPGIVGLLL TTLEYKSLDD CEELVINATA TINNLSYYQV 501 KNSIIQDKKL YIAELLLKLL VSNNMDGILE AVRVFGNLSQ DHDVCDFIVQ 551 NNVHRFMMAL LDAQHQDICF SACGVLLNLT VDKDKRVILK EGGGIKKLVD 601 CLRDLGPTDW QLACLVCKTL WNFSENITNA SSCFGNEDTN TLLLLLSSFL 651 DEELALDGSF DPDLKNYHKL HWETEFKPVA QQLLNRIQRH HTFLEPLPIP 701 SF

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_14p7, frame 2

TREMBL :MMD367_1 product: "KAP3B"; Mus musculus mRNA for KAP3B, complete eds., N = 2, Score = 97, P = 0.00039

>TREMBL:MMD367_1 product: "KAP3B"; Mus musculus mRNA for KAP3B, complete eds.

Length = 772

HSPs:

Score = 97 (14.6 bits), Expect = 3.9e-04, Sum P(2) = 3.9e-04 Identities = 45/163 (27%), Positives = 77/163 (47%)

Query: 442 LTRVLANIAIHPGVGPVLAANPGIVGLLLTTLEYKSLDDCEELVINATATINNLSYYQVK 501

L +++ NI+ H G P VG L + S D+ EE VI T+ NL+ + Sbjct: 483 LMKMIRNISQHDG—PTKNLFIDYVGDLAAQI SSDEEEEFVIECLGTLANLTIPDLD 537

Query: 502 -NSIIQDKKLYIAELLLKLLVSNNMDG-ILEAVRVFGNLSQDHDVCDFIVQNNVHRFMMA 559

++++ KL + L KL D +LE V + G +Ξ D + ++ + ++ Sbjct: 538 WELVLKEYKL-VPFLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIE 596

Query: 560 LLDAQHQDICFSACGVLL NLTVDKDKR-VILKEGGGIKKLVDCLRD 604

LL+AQ +D F C ++ + + R VI+KE L+D + D Sbjct: 597 LLNAQQEDDEF-VCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHD 644

Score = 77 (11.6 bits). Expect = 3.9e-04, Sum P(2) = 3.9e-04 Identities = 42/178 (23%), Positives = 82/178 (46%)

Query: 169 KLAKIILALKVSRKNLLNVCK-LIFKISRNEKNDSLIQNDSILESLLEVLRSEDLQTNME 227

K K L V ++ LL V L+ ++ + + + ++N +1+ L++ L + N E Sbjct: 263 KTFKKYQGLVVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRD NFE 318

Query: 228 AFLYCMGSIKFISGNLGFLNEMISKGAVEILINLIKQINENIKKCGTFLPNSGHLLVQVT 287

+ + +K +S + N+M+ VE L+ +1 +E++ L + +

Sbjct: 319 LLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDL LNITLR 366

Query: 288 ATLRNLVDSSLVRSKFLNISALPQLCTAM—EQYKGDKDVCT—NIARI—FSKLTSYRD 341

L D+ L R+K + + LP+L + E YK +C +1+ F + +Y D Sbjct: 367 LLLNLSFDTGL-RNKMVQVGLLPKLTALLGNENYK-QIAMCVLYHISMDDRFKSMFAYTD 424 Query: 342 CCTAL 346

C L Sbjct: 425 CIPQL 429

Score = 69 (10.4 bits). Expect = 2.6e+00, Sum P(2) = 9.2e-01 Identities = 35/146 (23%), Positives = 70/146 (47%)

Query: 512 IAELLLKLLVSNNMDGILEAVRVFGNLSQDHDVCDFIVQNNVHRFMMALLDAQHQDICFS 571

I +L+K L +N + ++ V LS + + +V+ ++ ++ ++ +H+D+ Sbjct: 304 IVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDLLNI 363

Query: 572 ACGVLLNLTVDKDKRVILKEGGGIKKLVDCLRDLGPTDW-QLACLVCKTLWNFSENITNA 630

+LLNL+ D R + + G + KL L G ++ Q+A +C L++ Ξ + Sbjct: 364 TLRLLLNLSFDTGLRNKMVQVGLLPKLTALL GNENYKQIA—MC-VLYHISMD-DRF 416

Query: 631 SSCFGNEDT-NTLLLLLSSFLDEELALD 657

S F D L+ +L DE + L+ Sbjct: 417 KSMFAYTDCIPQLMKMLFECSDERIDLE 444

Score = 68 (10.2 bits), Expect = 3.2e-03, Sum P(2) = 3.2e-03 Identities = 18/58 (31%), Positives = 30/58 (51%)

Query: 190 LIFKISRNEKN-DSLIQNDSILESLLEVLRΞE DLQTNMEAFLYCMGΞIKFIΞG 241

LI +++RN N + L+ N++ L +L VLR + +L TN+ +C S G Sbjct: 155 LILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSHFHG 212

Score = 65 (9.8 bits), Expect = 6.4e+00, Sum P(2) = l.Oe+00 Identities = 26/122 (21%), Positives = 53/122 (43%)

Query: 283 LVQVTATLRNL VDSSLVRSKFLNISALPQLCTAMEQYKGDKDVCTNIARIFSKLTS 338

+++ TL NL +D LV ++ +P L ++ + D+ + I S Sbjct: 521 VIECLGTLANLTIPDLDWELVLKEY KLVPFLKDKLKPGAAEDDLVLEVV-IMIGTVS 576

Query: 339 YRDCCTALAΞYSRCYALFLNLINKYQKKQDLVVRVVFILGNLTAKNNQAREQFSKEKGSI 398

D C AL + Ξ + L+N Q+ + V +++++ + + R+ KE + Sbjct: 577 MDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVF-HQATRDVIIKETQAP 635

Query: 399 QTLLSL 404

L+ L Sbjct: 636 AYLIDL 641

Score = 65 (9.8 bits), Expect = 6.4e+00, Sum P(2) = 1.0e+00 Identities = 44/177 (24%), Positives = 79/177 (44%)

Query: 481 CE-ELVINATATIN-NLSYYQ-VKNSIIQDKKLYIAELLLKLLVSNNMDGILEAVRVFGN 537

CE E ++N T + NLS+ ++N ++Q + + L LL + N I A+ V + Sbjct: 355 CEHEDLLNITLRLLLNLSFDTGLRNKMVQ VGLLPKLTALLGNENYKQI—AMCVLYH 409

Query: 538 LSQDHDVCD-FIVQNNVHRFMMALLDAQHQDICFSACGVLLNLTVDKDKRVILKEGGGIK 596

+S D F + + + M L + + 1 +NL +K ++ EG G+K Sbjct: 410 ISMDDRFKSMFAYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLK 469

Query: 597 KLVDCLRDLGPTDWQLACLVCKTLWNFSENITNASSCFGNEDTNTLLLLLSSFLDEELAL 656

L+ R L D L+ K + N S++ + F + L +SS +EE + Sbjct: 470 MLMK—RALKLKD PLLMKMIRNISQHDGPTKNLF-IDYVGDLAAQISSDEEEEFVI 522

Query: 657 D 657

+ Sbjct: 523 E 523

Score = 61 (9.2 bits), Expect = 1.6e-02, Sum P(2) = 1.6e-02 Identities = 20/66 (30%), Positives = 34/66 (51%)

Query: 304 LNISALPQLCTAM-EQYKGDKDVCTNIARIFSKLTSYRDCCTALASYSRCYALFLNLINK 362

LN +AL L + E +K ++ TNI IF +S+ + Y + AL +N+I+ Sbjct: 171 LNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSHFHGLITHY-KIGALCMNIIDH 229

Query: 363 YQKKQDL 369

K+ +L Sbjct: 230 ELKRHEL ₂36

Pedant information for DKFZphtes3_14p7, frame 2

Report for DKFZphtes3_14p7.2

[LENGTH] 708

[MW] 79266.35

[pi] 6.57 [FUNCAT] 30.25 vacuolar and lysosomal organization [S. cerevisiae, YEL013w] 3e-04

[FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YEL013w]

3e-04

[FUNCAT] 09.25 vacuolar and lysosomal biogenesis [S. cerevisiae, YEL013w] 3e-04

[BLOCKS] BL00923F Aspartate and glutamate racemases proteins

[BLOCKS] BL00288B Tissue inhibitors of metalloproteinases proteins

[PROSITE] MYRISTYL 9

[PROSITE] AMIDATION 1

[PROSITE] CK2_PHOSPHO_SITE 12

[PROSITE] PKC_PHOSPHO_SITE 7

[PROSITE] ASN_GLYCOSYLATION 11

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 7.49 %

SEQ ESKETVMMGDSMVKINGIYLTKΞNAICHLKSHPLQLTDDGGFSEIKEQEMFKGTTSLPSH SEG PRD cccceeeecccceeeccccccccceeeeecccccccccccccchhhhhhhhccccccccc

SEQ LKNGGDQGKRHARASSCPΞSSDLSRLQTKAVPKADLQEEDAEIEVDEVFWNTRIVPILRE SEG xxxxxxxxxx PRD cccccccchhhhhhcccccccchhhhhhhccccchhhhhhhhhhhcccccceeehhhhhh

SEQ LEKEENIETVCAACTQLHHALEEGNMLGNKFKGRSILLKTLCKLVDVGSDSLSLKLAKII

SEG xxxxxxxxxx

PRD hhhhhcchhhhhhhhhhhhhhhhcccccccccccccchhhhhheeeeccccchhhhhhhh

SEQ LALKVSRKNLLNVCKLIFKISRNEKNDSLIQNDSILESLLEVLRSEDLQTNMEAFLYCMG SEG xxxx PRD hhhhhhhhhhhhhhhhhccccccccccccccccchhhhhhhhhhhccchhhhhhhhhhcc

SEQ SIKFISGNLGFLNEMISKGAVEILINLIKQINENIKKCGTFLPNSGHLLVQVTATLRNLV SEG PRD ceeeeccccchhhhhhhcchhhhhhhhhhhhhcccccccccccccceeeeeehhhhhhhh

SEQ DSSLVRSKFLNISALPQLCTAMEQYKGDKDVCTNIARIFSKLTSYRDCCTALASYSRCYA SEG PRD ccchhhhheeeeccchhhhhhhhhhccccceeeehhhhhhhhhhcccchhhhhhhhhhhh

SEQ LFLNLINKYQKKQDLVVRVVFILGNLTAKNNQAREQFSKEKGSIQTLLSLFQTFHQLDLH

SEG PRD hhhhhhhhhhhhhhhheeeeeeeccccccchhhhhhhhhhhchhhhhhhhhhhhhhhhcc

SEQ SQKPVGQRGEQHRAQRPPSEAEDVLIKLTRVLANIAIHPGVGPVLAANPGIVGLLLTTLE SEG PRD ccccccccccccccccccccchhhhhhhhhhhhhhhccccccceeeccccchhhhhhhhh

SEQ YKSLDDCEELVINATATINNLSYYQVKNSIIQDKKLYIAELLLKLLVSNNMDGILEAVRV SEG xxxxxxxxxxxxx PRD hhccccchhhhhhhhheeeecccccccceeeehhhhhhhhhhhhhhhccccchhhhhhhh

SEQ FGNLSQDHDVCDFIVQNNVHRFMMALLDAQHQDICFSACGVLLNLTVDKDKRVILKEGGG SEG PRD cccccccccceeeeeecchhhhhhhhhhhhcccceeeecceeeeeeecccceeeeecccc

SEQ IKKLVDCLRDLGPTDWQLACLVCKTLWNFSENITNASSCFGNEDTNTLLLLLSSFLDEEL

SEG xxxxxxxxxxxxx

PRD hhhhhhhhhccccccccchhhhhhhhccccccccccccccccccccceeeehhhhhhhhh

SEQ ALDGSFDPDLKNYHKLHWETEFKPVAQQLLNRIQRHHTFLEPLPIPSF

SEG xxx

PRD hhccccccccchhhhhhhhhhchhhhhhhhhhhhhhhheeeecccccc

Prosite for DKFZphtes3_14p7.2

PS00001 206- ->210 ASN GLYCOSYLATION PDOC00001

PS00001 212- ->216 ASN GLYCOSYLATION PDOC00001

PΞ00001 311- ->315 ASN GLYCOSYLATION PDOC00001

PS00001 385- ->389 ASN GLYCOSYLATION PDOC00001

PS00001 493- ->497 ASN GLYCOSYLATION PDOC00001

PS00001 500- ->504 ASN GLYCOSYLATION PDOC00001

PS00001 543- ->547 ASN GLYCOSYLATION PDOC00001

PS00001 584- ->588 ASN GLYCOSYLATION PDOC00001

PS00001 628- ->632 ASN GLYCOSYLATION PDOC00001

PS00001 632- ->636 ASN GLYCOSYLATION PDOC00001

PS00001 635- ->639 ASN GLYCOSYLATION PDOC00001

PS00005 173- ->176 PKC PHOSPHO SITE PDOC00005

PS00005 186- ->189 PKC PHOSPHO SITE PDOC00005

PS00005 241- ->244 PKC PHOSPHO SITE PDOC00005 o o ca

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I I I I I I I I I l l o o o o o O O o o o 0 o o o x OT OT OT OT OT OT OT OT OT OT OT O O O O O O O O υ I l l l l I I I 1 I I υ υ ΓM x, x υ. o υ υ υ υ o υ

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DKFZphtes3 15al3

group: testes derived

DKFZphtes3_15al3 encodes a novel 387 amino acid protein with weak similarity to S. cerevisiae Hopl.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . similarity to S. cerevisiae Hopl complete cDNA, complete eds, potential start codon at Bp 116, 3 EST hits

S. cerevisiae Hoplp is a meiosis-specific protein

Sequenced by GBF

Locus: unknown

Insert length: 1848 bp

Poly A stretch at pos. 1766, no polyadenylation signal found

1 GGAAAGCGCA TGCGCGTCGG GCACAGCGCG TGCAGCCTCG TGCAGCTCTT

51 CTGGTCTCCG GCGCCCGCCC CTCAGACGTA ATGTTGAATT AAAGAAAATA

101 CTTTATCAGA AGAAGATGGC CACTGCCCAG TTGCAGAGGA CTCCCATGAG

151 TGCACTGGTA TTTCCCAATA AGATATCAAC TGAACACCAG TCTTTGGTGT

201 TAGTGAAGAG GCTTCTAGCA GTTTCAGTAT CCTGTATCAC GTATTTGAGG

251 GGAATATTCC CAGAATGCGC TTATGGAACA AGATATCTAG ATGATCTTTG

301 TGTCAAAATA CTGAGAGAAG ATAAAAATTG CCCAGGATCT ACACAGTTAG

351 TGAAATGGAT GCTAGGATGT TATGATGCTT TACAGAAAAA ATATGTATAC

401 ACAAACCCAG AAGATCCTCA GACAATTTCA GAATGTTACC AATTCAAATT

451 CAAATACACC AATAATGGAC CACTCATGGA CTTCATAAGT AAAAACCAAA

501 GCAACGAATC TAGCATGTTG TCTACTGACA CCAAGAAAGC AAGCATTCTC

551 CTCATTCGCA AGATTTATAT CCTAATGCAA AATCTGGGGC CTTTACCTAA

601 TGATGTTTGT TTGACCATGA AACTTTTTTA CTATGATGAA GTTACACCCC

651 CAGATTACCA GCCTCCCGGT TTTAAGGATG GTGATTGTGA AGGAGTTATA

701 TTTGAAGGGG AACCTATGTA TTTAAATGTG GGAGAAGTCT CAACACCTTT

751 TCACATCTTC AAAGTAAAAG TGACCACTGA GAGAGAACGA ATGGAAAATA

801 TTGACTCAAC TATACTATCA CCAAAACAAA TAAAAACACC ATTTCAAAAA

851 ATCCTGAGGG ACAAAGATGT AGAAGATGAA CAGGAGCATT ATACAAGTGA

901 TGATTTGGAC ATTGAAACTA AAATGGAAGA ACAGGAAAAA AACCCTGCAT

951 CTTCTGAACT TGAAGAACCA AGTTTAGTTT GTGAGGAAGA TGAAATTATG

1001 AGGTCTAAAG AAAGTCCAGA TCTTTCTATT TCTCATTCTC AGGTTGAGCA

1051 GTTAGTCAAT AAAACATCTG AACTTGATAT GTCTGAAAGC AAAACAAGAA

1101 GTGGAAAAGT CTTTCAGAAT AAAATGGCAA ATGGAAATCA ACCAGTAAAA

1151 TCTTCCAAAG AAAATCGGAA GAGAAGTCAA CATGAATCTG GGAGAATAGT

1201 CCTCCATCAC TTTGATTCTT CTAGTCAAGA GTCAGTGCCA AAAAGGAGAA

1251 AGTTTAGTGA ACCAAAGGAA CATATATAAA AATTATTTTT GTTCTGCAGG

1301 CTTGCAGAGT TCTTCTCACC ATTTAAACTG AAGGACCCTA TATTATATTT

1351 CCCTAACTCT GAAGATGTAT ATGTAGTTTA AAGCAGTTTG TACACTAAAA

1401 CTAAGTTTTT GGCTGACTGT CATATTGTGG TCCTTAATCT TGAGATAAAT

1451 CCAATAGAAC TTTTGAATAA AAGCAAAAGT ACAAATGTCA TAATTGATTC

1501 GGTAATAAGT AAAATTTCAA AATTGATTTT GTTCATTACC TACTTAATAT

1551 TTCCTTTAAA TATATACTAA CTGTTAAGGC CCTCTAATGC CATTTTTCTA

1601 AACAGTAATG TTTACTTTGG TATTAAAATT TGGTATGGAT TCACTTTTTA

1651 CTTATGTTAA AATTATACCA TTTAACTGGC TCTTTTGTCA TTGTGCTGTT

1701 ATTAAAACAA TGTTCTTCAA TATTTTGACA TAATGTATTA ACATTTTAAT

1751 ATATAATGTA CAATTTAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAGG

1801 GGCGGCCGCT CTAGAGGATC CAAGCTTACG TACAAAAAAA AAAAAAGG

BLAST Results o BLAST result

Medline entries o Medlme entry Peptide information for frame 2

ORF from 116 bp to 1276 bp; peptide length: 387 Category: similarity to known protein

1 MATAQLQRTP MSALVFPNKI STEHQSLVLV KRLLAVSVSC ITYLRGIFPE 51 CAYGTRYLDD LCVKILREDK NCPGSTQLVK WMLGCYDALQ KKYVYTNPED 101 PQTISECYQF KFKYTNNGPL MDFISKNQSN ESSMLSTDTK KASILLIRKI 151 YILMQNLGPL PNDVCLTMKL FYYDEVTPPD YQPPGFKDGD CEGVIFEGEP 201 MYLNVGEVST PFHIFKVKVT TERERMENID STILSPKQIK TPFQKILRDK 251 DVEDEQEHYT SDDLDIETKM EEQEKNPASS ELEEPSLVCE EDEIMRSKES 301 PDLSISHSQV EQLVNKTSEL DMSESKTRSG KVFQNKMANG NQPVKSSKEN 351 RKRSQHESGR IVLHHFDSSS QESVPKRRKF SEPKEHI

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_15al3, frame 2

TREMBL :ATAC2130_3 product: "F1N21.3"; The sequence of BAC F1N21 from Arabidopsis thaliana chromosome 1, complete sequence., N = 1, Score = 274, P = 5.7e-22

TREMBL :SC9877_9 gene: "hopl"; S. cerevisiae chromosome IX cosmid 9877., N = 2, Score = 126, P = 7. le-09

PIR:A34691 meiosis-specific protein HOP1 - yeast (Saccharomyces cerevisiae), N = 2, Score = 126, P = 7.8e-08

>TREMBL:ATAC2130_3 product: "F1N21.3"; The sequence of BAC F1N21 from Arabidopsis thaliana chromosome 1, complete sequence. Length = 562

HSPs:

Score = 274 (41.1 b ts), Expect = 5.7e-22, P = 5.7e-22 Identities = 84/290 (28%), Positives = 145/290 (50%)

Query: 22 TEHQSLVLVKRLLAVSVSCITYLRGIFPECAYGTRYLDDLCVKILREDKNCPGSTQLVKW 81

TE SL+L + LL +++ I+Y+RG+FPE + + + L +KI + S +L+ W Sbjct: 11 TEQDSLLLTRNLLRIAIFNISYIRGLFPEKYFNDKSVPALDMKIKKLMPMDAESRRLIDW 70

Query: 82 M-LGCYDALQKKYVYT NPEDPQTISECYQFKFKYTNNGP--LMDFIΞK—NQSN 130

M G YDALQ+KY+ T D I E Y F F Y+++ +M I++ N+ N

Sbjct: 71 MEKGVYDALQRKYLKTLMFSICETVDGPMIEE-YSFSFSYSDSDSQDVMMNINRTGNKKN 129

Query: 131 ESSMLST DTKKASILLIRKIYILMQNLGPLPNDVCLTMKLFYYDEVTPPDYQPP 184

ST + ++ ++R + LM+ L +P++ + MKL YYD+VTPPDY+PP Sbjct: 130 GGIFNSTADITPNQMRSSACKMVRTLVQLMRTLDKMPDERTIVMKLLYYDDVTPPDYEPP 189

Query: 185 GFKD—GDCEGVIFEGEPMYLNVGEVSTPFHIFKVKVTT ERERMENIDSTILS 235

F+ D ++ P+ + +G V++ + +KV + E + M++ D + Sbjct: 190 FFRGCTEDEAQYVWTKNPLRMEIGNVNSKHLVLTLKVKSVLDPCEDENDDMQD-DGKSIG 248

Query: 236 PKQIKTPFQKILRDKDVEDEQEHY TSDDLDIETKMEEQEKNPAΞSE 281

P + Q D ++ QE+ DD D E ++ ++PA +E

Sbjct: 249 PDSVHDD-QPSDSDSEISQTQENQFIVAPVEKQDDDDGEVDEDDNTQDPAENE 300

Pedant information for DKFZphtes3_15al3, frame 2

Report for DKFZphtes3_15al3.2

[LENGTH] 387

[MW] 44417.64

[pi] 5.57

[HOMOL] TREMBL :ATAC2130_3 product: "F1N21.3"; The sequence of BAC F1N21 from

Arabidopsis thaliana chromosome 1, complete sequence. 9e-23

[FUNCAT] 09.13 biogenesis of chromosome structure [S. cerevisiae, YIL072w] 7e-ll

[FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YIL072w] 7e-ll

[FUNCAT] 03.13 meiosis [S. cerevisiae, YIL072w] 7e-ll

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YIL072w] 7e-ll

[PIRKW] nucleus 2e-09

[ PIRKW] zmc finger 2e-09 [PIRKW] DNA binding 2e-09

[PROSITE] MYRISTYL 1

[PROSITE] CAMP_PHOSPHO_SITE 3

[PROSITE] CK2_PHOSPHO_SITE 12

[PROSITE] PKC_PHOSPHO_SITE 7

[PROSITE] ASN_GLYCOSYLATION 3

[KW] Alpha_Beta

SEQ MATAQLQRTPMSALVFPNKISTEHQSLVLVKRLLAVSVSCITYLRGIFPECAYGTRYLDD PRD cccccccccccccccccccchhhhhhhhhhhhhhhhhhhhheeeeecccccccccccchh

SEQ LCVKILREDKNCPGSTQLVKWMLGCYDALQKKYVYTNPEDPQTISECYQFKFKYTNNGPL PRD hhhhhhhccccccccccccccccchhhhhhhhhhhcccccccchhhhhheeeeeccccce

SEQ MDFISKNQSNESSMLSTDTKKASILLIRKIYILMQNLGPLPNDVCLTMKLFYYDEVTPPD PRD eeeecccccccceeecccchhhhhhhhhhhhhhhhhcccccccccceeeeeeeeeccccc

SEQ YQPPGFKDGDCEGVIFEGEPMYLNVGEVΞTPFHIFKVKVTTERERMENIDSTILSPKQIK PRD cccccccccccceeeeeccceeeeeccccccceeeeeecccchhhhhcccccccccchhh

SEQ TPFQKILRDKDVEDEQEHYTSDDLDIETKMEEQEKNPASSELEEPSLVCEEDEIMRΞKES PRD hhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhcccccccccccccchhhhhhhhhhcc

SEQ PDLSISHSQVEQLVNKTSELDMSESKTRSGKVFQNKMANGNQPVKSSKENRKRSQHESGR PRD ccccccchhhhhhhhhhcccccccccccccceeeeeccccccccchhhhhhhhhhcccce

SEQ IVLHHFDSSSQESVPKRRKFSEPKEHI PRD eeeeecccccccccccccccccccccc

Prosite for DKFZphtes3_15al3.2

PS00001 127->131 ASN_GLYCOSYLATION PDOC00001 PS00001 130->134 ASN_GLYCOSYLATION PDOC00001 PS00001 315->319 ASN_GLYCOSYLATION PDOC00001 PS00004 140-M44 CAMP_PHOSPHO_SITE PDOC00004 PS00004 351->355 CAMP_PHOSPHO_SITE PDOC00004 PS00004 378->382 CAMP_PHOSPHO_SITE PDOC00004 PS00005 139-M42 PKC_PHOSPHO_SITE PDOC00005 PS00005 167->170 PKC_PHOSPHO_SITE PDOC00005 PS00005 221->224 PKC_PHOSPHO_SITE PDOC00005 PS00005 235->238 PKC_PHOSPHO_SITE PDOC00005 PS00005 329->332 PKC_PHOSPHO_SITE PDOC00005 PS00005 346->349 PKC_PHOSPHO_SITE PDOC00005 PS00005 358->361 PKC_PHOSPHO_SITE PDOC00005 PS00006 96->100 CK2_PHOSPHO_SITE PDOC00006 PS00006 103->107 CK2_PHOSPHO_SITE PDOC00006 PS00006 177->181 CK2_PHOSPHO_SITE PDOC00006 PS00006 221->225 CK2_PHOSPHO_SITE PDOC00006 PS00006 260->264 CK2_PHOSPHO_SITE PDOC00006 PS00006 268->272 CK2_PHOSPHO_SITE PDOC00006 PS00006 280->284 CK2_PHOSPHO_SITE PDOC00006 PS00006 308->312 CK2_PHOSPHO_SITE PDOC00006 PS00006 318->322 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 346->350 CK2_PHOSPHO_SITE PDOC00006 PS00006 354->358 CK2_PHOSPHO_SITE PDOC00006 PS00006 369->373 CK2_PHOSPHO_SITE PDOC00006 PS00008 84->90 MYRISTYL PDOC00008

(No Pfam data available for DKFZphtes3 15al3.2) DKFZphtes3_15c24

group: metabolism

DKFZphtes3_15c24 encodes a novel 404 amino acid protein with strong similarity to 2- hydroxyacid dehydrogenases .

The novel protein contains a D-isomer specific 2-hydroxyacιd dehydrogenases signature. Proteins with such a signature have similar enzymatic activities: D-lactate dehydrogenase (EC 1.1.1.28), catalyzes the reduction of D-lactate to pyruvate. D-glycerate dehydrogenase (EC 1.1.1.29) catalyzes the reduction of hydroxypyruvate to glycerate. 3-phosphoglycerate dehydrogenase (EC 1.1.1.95), catalyzes the oxidation of D-3-phosphoglycerate to 3-phosphohydroxypyruvate . Therefore the novel protein is a new 2-hydroxyacιd dehydrogenase.

The new protein can find application in modulation of 2-hydroxyacιd dehydrogenases-dependent pathways and as a new enzyme for biotechnologic production processes. strong similarity to C. elegans T03F1.1 potential start at Bp 55 matches kozak consensus PyCCatgG

Sequenced by GBF

Locus : unknown

Insert length: 1956 bp

Poly A stretch at pos. 1929, polyadenylation signal at pos. 1903

1 CGAAGGCGGC GGCGAAGGCC CGGGCTGGGA GCGTTGGCGG CCGGAGTCCC

51 AGCCATGGCG GAGTCTGTGG AGCGCCTGCA GCAGCGGGTC CAGGAGCTGG

101 AGCGGGAACT TGCCCAGGAG AGGAGTCTGC AGGTCCCGAG GAGCGGCGAC

151 GGAGGGGGCG GCCGGGTCCG CATCGAGAAG ATGAGCTCAG AGGTGGTGGA

201 TTCGAATCCC TACAGCCGCT TGATGGCATT GAAACGAATG GGAATTGTAA

251 GCGACTATGA GAAAATCCGT ACCTTTGCCG TAGCAATAGT AGGTGTTGGT

301 GGAGTAGGTA GTGTGACTGC TGAAATGCTG ACAAGATGTG GCATTGGTAA

351 GTTGCTACTC TTTGATTATG ACAAGGTGGA ACTAGCCAAT ATGAATAGAC

401 TTTTCTTCCA ACCTCATCAA GCAGGATTAA GTAAAGTTCA AGCAGCAGAA

451 CATACTCTGA GGAACATTAA TCCTGATGTT CTTTTTGAAG TACACAACTA

501 TAATATAACC ACAGTGGAAA ACTTTCAACA TTTCATGGAT AGAATAAGTA

551 ATGGTGGGTT AGAAGAAGGA AAACCTGTTG ATCTAGTTCT TAGCTGTGTG

601 GACAATTTTG AAGCTCGAAT GACAATAAAT ACAGCTTGTA ATGAACTTGG

651 ACAAACATGG ATGGAATCTG GGGTCAGTGA AAATGCAGTT TCAGGGCATA

701 TACAGCTTAT AATTCCTGGA GAATCTGCTT GTTTTGCGTG TGCTCCACCA

751 CTTGTAGTTG CTGCAAATAT TGATGAAAAG ACTCTGAAAC GAGAAGGTGT

801 TTGTGCAGCC AGTCTTCCTA CCACTATGGG TGTGGTTGCT GGGATCTTAG

851 TACAAAACGT GTTAAAGTTT CTGTTAAATT TTGGTACTGT TAGTTTTTAC

901 CTTGGATACA ATGCAATGCA GGATTTTTTT CCTACTATGT CCATGAAGCC

951 AAATCCTCAG TGTGATGACA GAAATTGCAG GAAGCAGCAG GAGGAATATA

1001 AGAAAAAGGT AGCAGCACTG CCTAAACAAG AGGTTATACA AGAAGAGGAA

1051 GAGATAATCC ATGAAGATAA TGAATGGGGT ATTGAGCTGG TATCTGAGGT

1101 TTCAGAAGAG GAACTGAAAA ATTTTTCAGG TCCAGTTCCA GACTTACCTG

1151 AAGGAATTAC AGTGGCATAC ACAATTCCAA AAAAGCAAGA AGATTCTGTC

1201 ACTGAGTTAA CAGTGGAAGA TTCTGGTGAA AGCTTGGAAG ACCTCATGGC

1251 CAAAATGAAG AATATGTAGA TAATGGACTG GGATATATTG TATTTCTCAT

1301 GTTAAAGCCT CTTCCCTTGA AATTAAAAAA AAATTTTAAC TGATAAAACT

1351 TAGGGCAACA TTAATTAATG TATATTCTTA CCTGAATTGT TATACTTTTT

1401 GAAAATCCTG TGACTTGCCT GTTTCTCCCC GCTCCAACGA AATCATTAAC

1451 TCTCCTAAAA TGTGTTTCAT TCTAGTAAGA AAACCTCAAA GGATATTGTA

1501 GGATATAAAT CTTACTTGAA AACATAGCTG TTGAAATGTT TTGGCCTTTT

1551 GGAGTGGGGG AAGGACAAAT CTGATCCTGT AATCTTTTTC TTTCCAGTAA

1601 TCCCTTGTGT CTGTTGCATG AGGACATGGA CAATAAAGTA GTATATGATC

1651 CTCAGATACA GGGAGAAGGA CAAGGCATAC AGCTTATTGA TTAGAGCTGG

1701 CAAGCATCTG CTCATTATGT TTGGAATTGC TTTCTATAAG AAAATTGCCC

1751 ACTACTACTA ACTTGATCAA CAATGAATTC AAAATAGTTA ACCTATGAAA

1801 TAACATCCTC TCAAATGTTT GCTGATGAAG TACAAGTTGA AATGTAGTTA

1851 TTGGAAAAGT CTGTAACCTG TGGATCATAT ATATTCAAAG TGAGACAAAG

1901 GCAAATAAAA AGCAGCTATT TTCATGAATA GACAAAAAAA AAAAAAAAAA

1951 AAAAAG

BLAST Results o BLAST result Medline entries

No Medline entry

Peptide information for frame 1

ORF from 55 bp to 1266 bp; peptide length: 404 Category: similarity to unknown protein Classification: Metabolism Prosite motifs: D 2 HYDROXYACID DH 1 (76-105)

1 MAESVERLQQ RVQELERELA QERSLQVPRS GDGGGGRVRI EKMSSEVVDS

51 NPYSRLMALK RMGIVSDYEK IRTFAVAIVG VGGVGSVTAE MLTRCGIGKL

101 LLFDYDKVEL ANMNRLFFQP HQAGLSKVQA AEHTLRNINP DVLFEVHNYN

151 ITTVENFQHF MDRISNGGLE EGKPVDLVLS CVDNFEARMT INTACNELGQ

201 TWMESGVSEN AVSGHIQLII PGESACFACA PPLVVAANID EKTLKREGVC

251 AASLPTTMGV VAGILVQNVL KFLLNFGTVS FYLGYNAMQD FFPTMSMKPN

301 PQCDDRNCRK QQEEYKKKVA ALPKQEVIQE EEEIIHEDNE WGIELVSEVΞ

351 EEELKNFSGP VPDLPEGITV AYTIPKKQED SVTELTVEDS GESLEDLMAK 401 MKNM

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_15c24, frame 1

TREMBL :CEUT03F1_11 gene: "T03F1.1"; Caenorhabditis elegans cosmid T03F1., N = 1, Score = 1204, P = 1.9e-122

TREMBL :ATAC98_3 gene: "YUP8H12.3"; Arabidopsis thaliana chromosome 1 YAC yUP8H12 complete sequence., N = 1, Score = 733, P = 1.5e-72

PIR:A69319 thiamine biosynthesis protein (thiF) homolog - Archaeoglobus fulgidus, N = 1, Score = 218, P = 1.8e-17

TREMBL :AF022796_4 gene: "moeB"; product: "MoeB"; Staphylococcus carnosus molybdenum cofactor biosynthetic gene cluster, complete sequence., N = 1, Score = 220, P = 3.7e-16

>TREMBL:CEUT03F1_11 gene: "T03F1.1"; Caenorhabditis elegans cosmid T03F1. Length = 419

HSPs:

Score = 1204 (180.6 bits), Expect = 1.9e-122, P = 1.9e-122 Identities = 241/367 (65%), Positives = 293/367 (79%)

Query: 37 RVRIEKMSSEVVDSNPYSRLMALKRMGIVSDYEKIRTFAVAIVGVGGVGSVTAEMLTRCG 96

R +IEK+S+EVVDSNPYSRLMAL+RMGIV++YE+IR VA+VGVGGVGSV AEMLTRCG Sbjct: 48 RQKIEKLSAEVVDSNPYSRLMALQRMGIVNEYERIREKTVAVVGVGGVGSVVAEMLTRCG 107

Query: 97 IGKLLLFDYDKVELANMNRLFFQPHQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVEN 156

IGKL+LFDYDKVE+ANMNRLF+QP+QAGLSKV+AA TL ++NPDV EVHN+NITT++N Sbjct: 108 IGKLILFDYDKVEIANMNRLFYQPNQAGLSKVEAARDTLIHVNPDVQIEVHNFNITTMDN 167

Query: 157 FQHFMDRISNGGLEEGKPVDLVLSCVDNFEARMTINTACNELGQTWMESGVSENAVSGHI 216

F F++RI G L +GK +DLVLSCVDNFEARM +N ACNE Q WMESGVSENAVSGHI Sbjct: 168 FDTFVNRIRKGSLTDGK-IDLVLSCVDNFEARMAVNMACNEENQIWMESGVSENAVSGHI 226

Query: 217 QLIIPGESACFACAPPLVVAANIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNF 276

Q I PG++ACFAC PPLVVA+ IDE+TLKR+GVCAASLPTTM WAG LV N LK+LLNF Sbjct: 227 QYIEPGKTACFACVPPLVVASGIDERTLKRDGVCAASLPTTMAVVAGFLVMNTLKYLLNF 286

Query: 277 GTVSFYLGYNAMQDFFPTMSMKPNPQCDDRNCRKQQEEYKKKVAALPKQ-EV-IQEEEEI 334

G VS Y+GYNA+ DFFP S+KPNP CDD +C ++Q+EY++KVA P EV + EEE + Sbjct: 287 GEVSQYVGYNALSDFFPRDSIKPNPYCDDSHCLQRQKEYEEKVANQPVDLEVEVPEEETV 346

Query: 335 IHEDNEWGIELVSEVSEEELKNFSGPVPDLPEGITVAYTIPKKQEDSVTELTVEDSGESL 394

+HEDNEWGIELV+E SE + S + G+ AY P K+ D+ TEL+ + + Sbjct: 347 VHEDNEWGIELVNE-SEPSAEQSSSL—NAGTGLKFAYE-PIKR-DAQTELSPAQA—AT 399

Query: 395 EDLMAKMKN 403 D M +K+

Sbjct: 400 HDFMKSIKD 408

Pedant information for DKFZphtes3_15c24, frame 1

Report for DKFZphtes3_15c24.1

[LENGTH] 404 [MW] 44863.36 [pi] 4.79 [HOMOL] TREMBL:CEUT03F1_11 gene: "T03F1.1"; Caenorhabditis elegans cosmid T03F1. le-115

[FUNCAT] h cofactor metabolism [H. influenzae, HI1449] 2e-08

[FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YDR390c UBA2 - El-like]

4e-07

[FUNCAT] 04.05.05 mrna processing (5 '-end, 3' -end processing and mrna degradation) [S. cerevisiae YDR390C UBA2 - El-like] 4e-07

[FUNCAT] 06.13.01 cytoplasmic degradation [S. cerevisiae, YDR390c UBA2 - El-like]

4e-07

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YDR390c UBA2 - El-like] 4e-07

[FUNCAT] 11.01 stress response [S. cerevisiae, YKL210w UBA1 - El-like] 2e-06

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YKL210w UBA1 - El-like]

2e-06

[BLOCKS] BL01042A Homoserme dehydrogenase proteins

[PIRKW] thiamme pyrophosphate le-07

[PIRKW] molybdenum 5e-07

[PIRKW] molybdopterin biosynthesis 5e-07

[SUPFAM] molybdopteπn biosynthesis protein moeB 2e-12

[PROSITE] D_2_HYDROXYACID_DH_l 1

[KW] TRANSMEMBRANE 1

[KW] LOW COMPLEXITY 8.66 %

SEQ MAESVERLQQRVQELERELAQERSLQVPRSGDGGGGRVRIEKMSSEVVDSNPYSRLMALK

SEG

PRD ccchhhhhhhhhhhhhhhhhhhhhhhcccccccccccceeeccccccccccchhhhhhhc

MEM

SEQ RMGIVSDYEKIRTFAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQP

SEG xxxxxxxxx

PRD cccccchhhhhhhheeeeecccccchhhhhhhhhhcccceeeecccccchhhhhhhhhhc

MEM MMMMMMMMMMMMMMMMMMMMMM

SEQ HQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPVDLVLS

SEG

PRD ccccchhhhhhhhhhhhccccceeeeeccccccchhhhhhhhhhhcccccccccceeeee

MEM

SEQ CVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANID

SEG

PRD cccchhhhhhhhhhhhhhccccccccccccccccceeeeccccccceeeccccccccccc

MEM

SEQ EKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYNAMQDFFPTMSMKPN

SEG

PRD ccccccccccccccccchhhhhhhhhhhhhhhhhcccceeeccccccccccccccccccc

MEM

SEQ PQCDDRNCRKQQEEYKKKVAALPKQEVIQEEEEIIHEDNEWGIELVSEVSEEELKNFSGP

SEG xxxxxxxxxxxxxxx...xxxxxxxxxxx

PRD ccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccceeeeeehhhhhhhhhcccc

MEM

SEQ VPDLPEGITVAYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM

SEG

PRD ccccccceeeeeeehhhhhhhheeeeeccccchhhhhhhhhccc

MEM

Prosite for DKFZphtes3_15c24.1 PS00065 76->105 D_2_HYDROXYACID_DH_l PDOC00063

(No Pfam data available for DKFZphtes3_15c24.1) DKFZphtes3_15c6

group: transmembrane protein

DKFZphtes3_15c6 encodes a novel 118 amino acid protein without similarity to known proteins.

The novel protein contains 1 transmembrane region.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes and as a new marker for testicular cells. unknown complete cDNA, complete eds, EST hits

Sequenced by GBF

Locus : unknown

Insert length: 1283 bp

Poly A stretch at pos. 1264, no polyadenylation signal found

1 GAGACACTGA GCCCCGAGAC AGTGAGTGGT GGCCTCACTG CTCTGCCCGG 51 CACCCTGTCA CCTCCACTTT GCCTTGTTGG AAGTGACCCA GCCCCCTCCC

101 CTTCCATTCT CCCACCTGTT CCCCAGGACT CACCCCAGCC CCTGCCTGCC

151 CCTGAGGAAG AAGAGGCACT CACCACTGAG GACTTTGAGT TGCTGGATCA

201 GGGGGAGCTG GAGCAGCTGA ATGCAGAGCT GGGCTTGGAG CCAGAGACAC

251 CGCCAAAACC CCCTGATGCT CCACCCCTGG GGCCCGACAT CCATTCTCTG

301 GTACAGTCAG ACCAAGAAGC TCAGGCCGTG GCAGAGCCAT GAGCCAGCCG

351 TTGAGGAAGG AGCTGCAGGC ACAGTAGGGC TTCCTGGCTA GGAGTGTTGC

401 TGTTTCCTCC TTTGCCTACC ACTCTGGGGT GGGGCAGTGT GTGGGGAAGC

451 TGGCTGTCGG ATGGTAGCTA TTCCACCCTC TGCCTGCCTG CCTGCCTGCT

501 GTCCTGGGCA TGGTGCAGTA CCTGTGCCTA GGATTGGTTT TAAATTTGTA

551 AATAATTTTC CATTTGGGTT AGTGGATGTG AACAGGGCTA GGGAAGTCCT

601 TCCCACAGCC TGCGCTTGCC TCCCTGCCTC ATCTCTATTC TCATTCCACT

651 ATGCCCCAAG CCCTGGTGGT CTGGCCCTTT CTTTTTCCTC CTATCCTCAG

701 GGACCTGTGC TGCTCTGCCC TCATGTCCCA CTTGGTTGTT TAGTTGAGGC

751 ACTTTATAAT TTTTCTCTTG TCTTGTGTTC CTTTCTGCTT TATTTCCCTG

801 CTGTGTCCTG TCCTTAGCAG CTCAACCCCA TCCTTTGCCA GCTCCTCCTA

851 TCCCGTGGGC ACTGGCCAAG CTTTAGGGAG GCTCCTGGTC TGGGAAGTAA

901 AGAGTAAACC TGGGGCAGTG GGTCAGGCCA GTAGTTACAC TCTTAGGTCA

951 CTGTAGTCTG TGTAACCTTC ACTGCATCCT TGCCCCATTC AGCCCGGCCT 1001 TTCATGATGC AGGAGAGCAG GGATCCCGCA GTACATGGCG CCAGCACTGG 1051 AGTTGGTGAG CATGTGCTCT CTCTTGAGAT TAGGAGCTTC CTTACTGCTC 1101 CTCTGGGTGA TCCAAGTGTA GTGGGACCCC CTACTAGGGT CAGGAAGTGG 1151 ACACTAACAT CTGTGCAGGT GTTGACTTGA AAAATAAAGT GTTGATTGGC 1201 TAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAGGGCGGCC GCTCTAGAGG 1251 ATCCAAGCTT ACGTAAAAAA AAAAAAAAAA AAG

BLAST Results

NO BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 461 bp to 814 bp; peptide length: 118 Category: putative protein

1 MVAIPPSACL PACCPGHGAV PVPRIGFKFV NNFPFGLVDV NRAREVLPTA 51 CACLPASSLF SFHYAPSPGG LALSFSSYPQ GPVLLCPHVP LGCLVEALYN 101 FSLVLCSFLL YFPAVSCP BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_15c6, frame 2

PIR:S54250 ribosomal protein L2 - Arabidopsis thaliana, N = 1, Score = 76, P = 0.33

>PIR:S54250 ribosomal protein L2 - Arabidopsis thaliana Length = 258

HSPs:

Score = 76 (11.4 bits), Expect = 4.0e-01, P = 3.3e-01 Identities = 30/91 (32%), Positives = 4/91 (48%)

Query: 15 PGHGAVPVPRIGFKFVNNFPFGLVDVNRAREVLPTACACLPASSLFSFHYAPSPGGLALS 74

PG GA P+ R+ F+ PF + +E+ A C P ΞSL+ A G L Sbjct: 52 PGRGA-PLARVTFRH PFRF KKQKELFVAAEVCTPVSSLYCGKKATLVVGNVLP 103

Query: 75 FSSYPQGPVLLCP HV-PLGCLVEALYNFSLVL 105

S P+G V+ C HV G L A ++++V+ Sbjct: 104 LRSIPEGAVV-CNVEHHVGDRGVLARASGDYAIVI 137

Pedant information for DKFZphtes3_15c6, frame 2

Report for DKFZphtes3_15c6.2

[LENGTH] 118

[MW] 12413.79

[pi] 7.53

[PROSITE] LEUCINE_ZIPPER 1

[PROSITE] MYRISTYL 1

[PROSITE] ASN_GLYCOSYLATION

[KW] TRANSMEMBRANE 1

SEQ MVAIPPSACLPACCPGHGAVPVPRIGFKFVNNFPFGLVDVNRAREVLPTACACLPAΞSLF PRD cccccccccccccccccccccccccceeeecccccceeehhhhhhccccceeeccccccc MEM

SEQ SFHYAPSPGGLALSFSSYPQGPVLLCPHVPLGCLVEALYNFSLVLCSFLLYFPAVSCP PRD eeecccccccceeeeecccccccccccccccchhhhhhhcchhhhhhhhccccccccc MEM MMMMMMMMMMMMMMMMM.

Prosite for DKFZphtes3_15c6.2

PS00001 100->104 ASN_GLYCOSYLATION PDOC00001 PS00008 70->76 MYRISTYL PDOC00008

PS00029 84->106 LEUCINE ZIPPER PDOC00029

(No Pfam data available for DKFZphtes3_15c6.2)

DKFZphtes3_15gl4

group: testes derived

DKFZphtes3_15gl4 encodes a novel 701 amino acid protein with weak similarity to S. cerevisiae hypothetical protein YOR243c.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes. similarity to YOR243c complete cDNA, complete eds, potential start codon at Bp 35, EST hits

Sequenced by GBF

Locus : unknown

Insert length: 3495 bp

Poly A stretch at pos. 3462, no polyadenylation signal found

1 GCCTTCCACT GAACCGAGGC ACTGTTATAG AAGAATGGAA GAAGATACAG

51 ATTATAGAAT CAGGTTTAGT TCTTTGTGTT TCTTTAATGA TCACGTTGGA

101 TTTCATGGCA CTATAAAAAG CTCACCAAGT GACTTTATTG TTATTGAAAT

151 TGATGAACAG GGACAGTTAG TTAATAAGAC CATCGATGAG CCTATTTTCA

201 AGATTAGTGA AATACAACTT GAGCCAAATA ATTTTCCCAA AAAACCAAAA

251 CTAGATCTTC AAAATCTGTC CTTAGAAGAT GGAAGAAACC AAGAAGTTCA

301 TACTTTGATT AAGTACACTG ATGGTGACCA AAATCATCAG TCTGGTTCAG

351 AAAAGGAAGA TACTATCGTT GATGGAACTT CCAAATGTGA AGAAAAAGCT

401 GATGTTTTAA GCTCCTTTTT GGATGAAAAA ACTCATGAGT TACTGAATAA

451 TTTTGCCTGT GATGTAAGAG AGAAGTGGCT TTCTAAAACA GAGCTAATTG

501 GACTACCTCC TGAATTCTCA ATAGGCAGAA TCCTTGACAA AAACCAGAGG

551 GCTAGTTTAC ACAGTGCCAT TAGGCAGAAA TTTCCATTTT TAGTAACTGT

601 AGGAAAAAAC AGTGAAATTG TTGTAAAACC AAATCTTGAA TATAAAGAAC

651 TTTGTCATTT GGTATCTGAA GAGGAAGCAT TTGACTTTTT TAAATATTTG

701 GATGCAAAGA AAGAAAATTC CAAATTTACC TTTAAACCTG ATACAAACAA

751 AGACCACAGA AAAGCTGTCC ACCATTTTGT CAACAAAAAG TTTGGAAACC

801 TTGTGGAAAC CAAATCTTTT TCTAAAATGA ATTGCAGTGC TGGTAATCCG

851 AATGTGGTGG TAACAGTAAG ATTTCGGGAA AAAGCACACA AACGTGGGAA

901 AAGGCCTCTT TCTGAATGCC AAGAAGGAAA AGTTATATAT ACAGCTTTTA

951 CCCTACGAAA GGAAAACCTG GAAATGTTTG AAGCGATTGG TTTTTTAGCT

1001 ATCAAACTTG GTGTTATTCC TTCGGATTTT AGTTATGCAG GCCTTAAAGA

1051 CAAGAAAGCC ATCACCTATC AAGCAATGGT TGTTAGAAAA GTGACTCCAG

1101 AGAGGTTGAA AAATATTGAA AAAGAAATTG AAAAGAAAAG AATGAATGTC

1151 TTTAATATTC GGTCTGTAGA TGATTCCCTG AGACTTGGTC AGCTCAAAGG

1201 AAATCACTTT GATATTGTCA TTAGAAATTT AAAAAAACAA ATAAATGATT

1251 CTGCAAACCT GAGGGAGAGA ATTATGGAAG CAATAGAAAA TGTTAAGAAA

1301 AAAGGCTTTG TGAATTACTA TGGACCACAG AGATTTGGGA AGGGAAGGAA

1351 AGTTCACACA GACCAAATTG GACTAGCTTT GCTGAAGAAT GAAATGATGA

1401 AAGCCATAAA ATTGTTTCTT ACACCAGAAG ACTTGGATGA TCCTGTAAAT

1451 AGAGCAAAGA AGTATTTTCT TCAAACTGAG GATGCTAAAG GCACACTTTC

1501 ATTGATGCCT GAATTCAAAG TGCGTGAGAG AGCATTGTTG GAGGCATTGC

1551 ACCGCTTTGG CATGACCGAG GAAGGTTGTA TCCAGGCATG GTTCTCTTTA

1601 CCCCATTCCA TGCGCATATT CTATGTTCAC GCATATACCA GCAAAATTTG

1651 GAATGAGGCA GTATCTTACA GACTTGAAAC CTATGGAGCA AGAGTAGTGC

1701 AGGGTGATTT GGTCTGTTTG GATGAAGACA TTGATGACGA GAATTTCCCA

1751 AATAGTAAAA TTCACCTGGT AACTGAAGAG GAGGGATCAG CTAATATGTA

1801 TGCAATACAT CAGGTGGTTC TTCCAGTACT TGGATACAAT ATTCAGTACC

1851 CGAAGAACAA AGTAGGGCAG TGGTACCATG ACATACTTAG CAGAGATGGA

1901 CTACAGACAT GTAGGTTTAA AGTACCTACT CTGAAACTGA ATATACCAGG

1951 TTGCTATAGA CAGATTTTGA AACATCCCTG TAATCTCTCA TACCAACTAA

2001 TGGAAGATCA TGACATTGAT GTCAAAACGA AAGGTTCCCA CATTGATGAA

2051 ACAGCTTTGT CTCTTTTGAT CTCTTTTGAT CTTGATGCTT CATGCTATGC

2101 TACCGTTTGT CTGAAGGAAA TAATGAAGCA TGACGTTTAA AACTGATACC

2151 CTTGGTATAA CCATATATAT GTCACCCTTT CCTGTTTTTG AAATTATTGA

2201 TCAGAACAAT ATACAAGGGA AATGCCATAC CTCTGTTTGT GATAGATACC

2251 CCAGAGTAGT TATTACCTCT TTGTGAGATA AGTAATCTTT GATGAAGATT

2301 GAAATACAAT TTCTCATCCA ATTTTTATAT CTTGGCATAC GCTGACCCTC

2351 TTGACCATTT GTAATTTTTT CATATTATCT AAAACAGGTG TTAGAGTCAG

2401 ACAGATTCAT TCTTAGATTC TAGCTCTGAC ACTTACTAGT GATTTTGAGT

2451 ATGTTGTTGA TTTTTTTGTG TGTGGTTACT GATAGAATCA AGACAATTAC

2501 AACTTCATAA ATGACAAATA ATAGGATTAT CTCCACATTT TCTGTTGCTG

2551 GAGGAACAAA ACATTGTGCC CATTTGAAAA TTTTAATTTT TGTTGGTTTA

2601 ACTATCCCAC ATTATAAATC ATCCTTCACC ATTTTATATC AGTTAAATAT

2651 GGGTGTGTTG GGGAGGAATG ACTGGCATGT AGACATGTAT TGATTTAGGA

2701 AGATCTGAGC ATTTCTTTCA TTGTTGGTAA GATATAATGA TGAAATTTAA 2751 AAAGCAGTAT GGAGCATTAT ATATCAGTAA TGTGATATAT ATACTTAAGC

2801 CAGTTTAACC ATTTTGGGAA ATGTTAGCAT TAGGAAATAA AATCCAAAAG

2851 AAGGAAGAGA AGCTATATGC AATGCAAAAT TTGCTTATTG CAATATTTTC

2901 ATATACAGAC ACTAAAAACA GTTTTCAAAG TCCAGCATTA CGTAACTAAA

2951 GTAAGTAAAA TGATGTGTAT CAACTTGATG GTAAAATATG TAGTTATTTA

3001 AAAAAGCAAT GAACAATTTA GTTTCATGAG AAAATGTTGC CCCCTAAAAG

3051 TAGAACACAT ATGTTACAAC TGCAATAATA CTCTGAATTC ATCTTTCACA

3101 AATAAGAGAC ATGTTAGCAT AGTGATTAAA AGCACAGATA TTGGAGACAA

3151 ACTAACCCAG TTTGAACCCT GGCACTGCCA CGTATAGCAC TGCAGCCTTG

3201 GGAAAGTTAT TTAAACTCAT GGGCTTCAGT TTCAACATCT GTAAAATGGG

3251 CATGTTAACA TTGCCTACCT CATAGGATTA CTGTGAGAAT TTTCTAAGTT

3301 AATATATGTA AAGCAACTTT AAAAAGTGCC TGGCACTTAG TTATTGTTAA

3351 GTAAGTGTCT GCAGATGCAA GTTTGGAAGA GAAAAGCAAA TAAATGAAAA

3401 TCCCTTCCTG TTAAGATGAA AAAAAAAAAA AAAAAAAAAA AAAAAAGGGG

3451 CGGCCGCTCA AGATGAAAAA AAAAAAAAAA AAAAAAAAAA AAAGG

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 35 bp to 2137 bp; peptide length: 701 Category: similarity to unknown protein

1 MEEDTDYRIR FSSLCFFNDH VGFHGTIKSS PSDFIVIEID EQGQLVNKTI 51 DEPIFKISEI QLEPNNFPKK PKLDLQNLSL EDGRNQEVHT LIKYTDGDQN 101 HQSGSEKEDT IVDGTSKCEE KADVLSSFLD EKTHELLNNF ACDVREKWLS 151 KTELIGLPPE FSIGRILDKN QRASLHSAIR QKFPFLVTVG KNSEIVVKPN 201 LEYKELCHLV SEEEAFDFFK YLDAKKENSK FTFKPDTNKD HRKAVHHFVN 251 KKFGNLVETK SFSKMNCSAG NPNVVVTVRF REKAHKRGKR PLSECQEGKV 301 IYTAFTLRKE NLEMFEAIGF LAIKLGVIPS DFSYAGLKDK KAITYQAMVV 351 RKVTPERLKN IEKEIEKKRM NVFNIRSVDD SLRLGQLKGN HFDIVIRNLK 401 KQINDSANLR ERIMEAIENV KKKGFVNYYG PQRFGKGRKV HTDQIGLALL 451 KNEMMKAIKL FLTPEDLDDP VNRAKKYFLQ TEDAKGTLSL MPEFKVRERA 501 LLEALHRFGM TEEGCIQAWF SLPHSMRIFY VHAYTSKIWN EAVSYRLETY 551 GARVVQGDLV CLDEDIDDEN FPNSKIHLVT EEEGSANMYA IHQVVLPVLG 601 YNIQYPKNKV GQWYHDILSR DGLQTCRFKV PTLKLNIPGC YRQILKHPCN 651 LSYQLMEDHD IDVKTKGSHI DETALΞLLIS FDLDASCYAT VCLKEIMKHD 701 V

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_15gl4, frame 2

TREMBL :SPBC1A45P_10 gene: "SPBC1A4.09"; product: "hypothetical protein"; S. pombe chromosome II cosmid clA4 left hand region 1-26184 bp Originates from chimeric cosmid., N = 3, Score = 511, P = 2.9e-57

PIR:S67136 hypothetical protein YOR243c - yeast (Saccharomyces cerevisiae), N = 2, Score = 516, P = 7.3e-54

SWISSPROT :YQ4B_CAEEL HYPOTHETICAL 64.6 KD PROTEIN B0024.ll IN CHROMOSOME V., N = 2, Score = 386, P = 2.1e-34

>PIR:S67136 hypothetical protein YOR243c - yeast (Saccharomyces cerevisiae) Length = 676

HSPs:

Score = 516 (77.4 bits), Expect = 7.3e-54, Sum P(2) = 7.3e-54 Identities = 151/498 (30%), Positives = 245/498 (49%)

Query: 191 KNSEIVVKPNLEYKELCHLVSEEEAFDFFK-YLDAKKENSKFTFKPDTNKDHRKAVHHFV 249 + E V P L +L + EE+ Y A K + F+ +K R +H + Sbjct: 109 RRQEFNVDPELR-NQLVEIFGEEDVLKIESVYRTANKMETAKNFE DKSVRTKIHQLL 164

Query: 250 NKKFGNLVETKSFSKMNCSAGNPNVVVTVRFREKAHK-RGKRPLSECQEG-KVIYTAFTL 307

+ F N +E+ + N +EK ++ R + G + FTL

Sbjct: 165 REAFKNELESVTTDTNTFKIARSNRNSRTNKQEKINQTRDANGVENWGYGPSKDFIHFTL 224

Query: 308 RKENLEMFEAIGFLAIKLGVIPSD-FSYAGLKDKKAITYQAMVVRKVTPERLKNIEKEIE 366

KEN + EA+ + KL +PS YAG KD++A+T Q + + K+ +RL + + + Sbjct: 225 HKENKDTMEAVNVIT-KLLRVPSRVIRYAGTKDRRAVTCQRVSISKIGLDRLNALNRTL- 282

Query: 367 KKRMNVFNIRSVDDSLRLGQLKGNHFDIVIRNLKKQINDSANLRERIMEAIENVKKKGFV 426

K M + N D SL LG LKGN F +VIR++ N +L E + +++ + GF+ Sbjct: 283 -KGMIIGNYNFSDASLNLGDLKGNEFVVVIRDVTTG-NSEVSLEEIVSNGCKSLSENGFI 340

Query: 427 NYYGPQRFGKGRKVHTDQIGLALLKNEMMKAIKLFLTPEDLDDPVNR-AKKYFLQTEDAK 485

NY+G QRFG + T IG LL + KA +L L+ +D P ++ A+K + +T+DA Sbjct: 341 NYFGMQRFGTF-SISTHTIGRELLLSNWKKAAELILSDQDNVLPKSKEARKIWAETKDAA 399

Query: 486 GTLSLMPEFKVRERALLEALHRFGMTEEGCIQ—AWFS LPHSMRIFYVHAYTSKIW 539

L MP + E ALL +L E+G A+++ +P ++R YVHAY S +W Sbjct: 400 LALKQMPRQCLAENALLYSLSNQRKEEDGTYSENAYYTAIMKIPRNLRTMYVHAYQSYVW 459

Query: 540 NEAVSYRLETYGARVVQGDLVC LDEDIDDENFPNS KIHLVTEEEGS 585

N S R+E +G ++V GDLV L IDDE+F + VT+E+

Sbjct: 460 NSIASKRIELHGLKLVVGDLVIDTSEKSPLISGIDDEDFDEDVREAQFIRAKAVTQEDID 519

Query: 586 ANMYAIHQVVLPVLGYNIQYPKNK-VGQWYHDILSRDGLQTCRFKVPTLKLNIPGCYRQI 644

+ Y + VVLP G+++ YP N+ + Q Y DIL D + + ++ G YR + Sbjct: 520 SVKYTMEDVVLPSPGFDVLYPSNEELKQLYVDILKADNMDPFNMRRKVRDFSLAGΞYRTV 579

Query: 645 LKHPCNLSYQLMEDHDIDVKTKGSHID 671

++ P +L Y+++ D + + +D Sbjct: 580 IQKPKSLEYRIIHYDDPSQQLVNTDLD 606

Score = 86 (12.9 bits), Expect = 3.2e-01, Sum P(2) = 2.8e-01 Identities = 40/160 (25%), Positives = 77/160 (48%)

Query: 22 GFHGTIKSSPSDFIVIEIDEQGQLVNKTIDEPIFKISEIQLEPNNFPKKPKLDLQNLSLE 81

GF G IK +DF+V EID++G++++ T D+ FK+ + +P K +++ + S E Sbjct: 55 GFRGQIKQRYTDFLVNEIDQEGKVIHLT-DKG-FKMPK KPQR—SKEEVNAEKES-E 106

Query: 82 DGRNQEVHTLIKYTDGDQNHQSGS—EKEDTI-VDGTSKCEEKADVLSSFLDEKTHELLN 138

R QE + D + +Q +ED + ++ + K + +F D+ ++ Sbjct: 107 AARRQEFNV DPELRNQLVEIFGEEDVLKIESVYRTANKMETAKNFEDKSVRTKIH 161

Query: 139 NFACDVREKWLSKTELIGLPPE-FSIGRILDKNQRASLHSAIRQ 181

+RE + ++ E + F I R ++N R + I Q Sbjct: 162 QL LREAFKNELESVTTDTNTFKIARS-NRNSRTNKQEKINQ 201

Score = 58 (8.7 bits), Expect = 7.3e-54, Sum P(2) = 7.3e-54 Identities = 10/23 (43%), Positives = 17/23 (73%)

Query: 676 SLLISFDLDASCYATVCLKEIMK 698

++++ F L S YAT+ L+E+MK Sbjct: 638 AVVLKFQLGTSAYATMALRELMK 660

Pedant information for DKFZphtes3_15gl4, frame 2

Report for DKFZphtes3_15gl4.2

[LENGTH] 701

[MW] 80700.96

[pi] 7.31

[HOMOL] PIR:S67136 hypothetical protein YOR243c - yeast (Saccharomyces cerevisiae) 2e-

51

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YOR243c] 8e-53

[BLOCKS] BL01268C

[BLOCKS] BL01268B

[BLOCKS] BL01268A

[SUPFAM] hypothetical protein HI0701 3e-06

[PROSITE] MYRISTYL 7

[PROSITE] AMIDATION 2

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 16

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 13

[PROSITE] ASN_GLYCOSYLATION 5

[KW] Alpha_Beta SEQ MEEDTDYRIRFSSLCFFNDHVGFHGTIKSSPSDFIVIEIDEQGQLVNKTIDEPIFKISEI PRD ccccceeeeeecceeecccccccceeeeecccceeeeeecccceeeeeccccceeeeeee

SEQ QLEPNNFPKKPKLDLQNLSLEDGRNQEVHTLIKYTDGDQNHQSGSEKEDTIVDGTSKCEE PRD cccccccccccccccccccccccccccccceeeeccccccccccccceeeeeecccccch

SEQ KADVLSSFLDEKTHELLNNFACDVREKWLSKTELIGLPPEFSIGRILDKNQRASLHSAIR PRD hhhhhhhhhhhhhhhhhhhcchhhhhhhhhhheeecccccceeeeeeecchhhhhhhhhh

SEQ QKFPFLVTVGKNSEIVVKPNLEYKELCHLVSEEEAFDFFKYLDAKKENSKFTFKPDTNKD PRD hhccceeeecccceeeecccchhhhhhhhhhhhhhhhhhhhhhcccccceeeecccccch

SEQ HRKAVHHFVNKKFGNLVETKSFSKMNCSAGNPNVVVTVRFREKAHKRGKRPLSECQEGKV PRD hhhhhhhhhhhhhhheeeeecccceeeecccccceeeechhhhhhhhcccccccccccce

SEQ IYTAFTLRKENLEMFEAIGFLAIKLGVIPSDFSYAGLKDKKAITYQAMVVRKVTPERLKN PRD eeeeeeeeccccchhhhhhhhhhhhcccccceeeccccchhhhhhhheeeccccchhhhh

SEQ IEKEIEKKRMNVFNIRSVDDSLRLGQLKGNHFDIVIRNLKKQINDSANLRERIMEAIENV PRD hhhhhhhhhheeeeeeccccccccccccccceeeeeehhhhhccccchhhhhhhhhhhhh

SEQ KKKGFVNYYGPQRFGKGRKVHTDQIGLALLKNEMMKAIKLFLTPEDLDDPVNRAKKYFLQ PRD hhcccccccccccccccccccchhhhhhhhhhhhhhhhhhhhcccccccchhhhhhhhhh

SEQ TEDAKGTLSLMPEFKVRERALLEALHRFGMTEEGCIQAWFSLPHSMRIFYVHAYTSKIWN PRD hcccchhhhhhhhhhhhhhhhhhhhhhcccccchhhhhhhhcccchhhhhhhhhhhhhhh

SEQ EAVSYRLETYGARVVQGDLVCLDEDIDDENFPNSKIHLVTEEEGSANMYAIHQVVLPVLG PRD hhhhhhhhhhcceeeccceeeeccccccccccccccceeecccccccccccceeeccccc

SEQ YNIQYPKNKVGQWYHDILSRDGLQTCRFKVPTLKLNIPGCYRQILKHPCNLSYQLMEDHD PRD cccccccccchhhhhhhhhhccccccccccccccccccchhhhhhhhccchhhhhhhhcc

SEQ IDVKTKGSHIDETALSLLISFDLDASCYATVCLKEIMKHDV PRD ceeeccccchhhhhhheeeeeecccccchhhhhhhhhhccc

Prosite for DKFZphtes3_15gl4.2

PS00001 47->51 ASN_GLYCOSYLATION PDOC00001 PS00001 77->81 ASN_GLYCOSYLATION PDOC00001 PS00001 266->270 AΞN_GLYCOSYLATION PDOC00001 PS00001 404->408 AΞN_GLYCOSYLATION PDOC00001 PS00001 650->654 ASN_GLYCOSYLATION PDOC00001 PS00004 351->355 CAMP_PHOSPHO_SITE PDOC00004 PS00005 26->29 PKC_PHOSPHO_SI E PDOC00005 PS00005 105->108 PKC_PHOSPHO_SITE PDOC00005 PS00005 115->118 PKC_PHOSPHO_SITE PDOC00005 PS00005 232->235 PKC_PHOSPHO_SITE PDOC00005 PS00005 237->240 PKC_PHOSPHO_SITE PDOC00005 PS00005 277->280 PKC_PHOSPHO_SITE PDOC00005 PS00005 306->309 PKC_PHOSPHO_SITE PDOC00005 PS00005 381->384 PKC_PHOSPHO_SITE PDOC00005 PS00005 525->528 PKC_PHOSPHO_SITE PDOC00005 PS00005 535->538 PKC_PHOSPHO_SITE PDOC00005 PS00005 544->547 PKC_PHOSPHO_SITE PDOC00005 PS00005 625->628 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 632->635 PKC_PHOSPHO_SITE PDOC00005 PS00006 30->34 CK2_PHOSPHO_SITE PDOC00006 PS00006 49->53 CK2_PHOSPHO_SITE PDOC00006 PS00006 79->83 CK2_PHOSPHO_SITE PDOC00006 PS00006 95->99 CK2_PHOSPHO_SITE PDOC00006 PS00006 103->107 CK2_PHOSPHO_SITE PDOC00006 PS00006 105->109 CK2_PHOSPHO_SITE PDOC00006 PS00006 110->114 CK2_PHOSPHO_SITE PDOC00006 PS00006 116->120 CK2_PHOSPHO_SITE PDOC00006 PS00006 127->131 CK2_PHOSPHO_SITE PDOC00006 PS00006 150->154 CK2_PHOSPHO_SITE PDOC00006 PS00006 211->215 CK2_PHOSPHO_SITE PDOC00006 PS00006 237->241 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 377->381 CK2_PHOSPHO_SITE PDOC00006 PS00006 463->467 CK2_PHOSPHO_SITE PDOC00006 PS00006 580->584 CK2_PHOSPHO_SITE PDOC00006 PS00006 668->672 CK2_PHOSPHO_SITE PDOC00006 PS00007 537->546 TYR_PHOSPHO_SITE PDOC00007 PS00008 25->31 MYRISTYL PDOC00008 PS00008 43->49 MYRISTYL PDOC00008 PS00008 114->120 MYRISTYL PDOC00008 PS00008 326->332 MYRISTYL PDOC00008 PS00008 385->391 MYRISTYL PDOC00008 PS00008 514->520 MYRISTYL PDOC00008 PΞ00008 622->628 MYRISTYL PDOC00008 PS00009 287->291 AMIDATION PDOC00009 PS00009 436->440 AMIDATION PDOC00009

(No Pfam data available for DKFZphtes3_15gl4.2)

DKFZphtes3_15hl

group: testes derived

DKFZphtes3_15hl encodes a novel 672 amino acid protein with very weak similarity to several proteins .

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes. similarity to Hsp70/Hsp90 organizing protein complete cDNA, complete eds, no EST hits

Sequenced by GBF

Locus : unknown

Insert length: 2277 bp

Poly A stretch at pos. 2252, polyadenylation signal at pos. 2226

1 AAACCAGATA GAGGTTCTCC AGCTTTTCTT TGATTGTCTC TGCTTTAGCG 51 TCTCTAAATC CGGTCACCAT GTCGGACCCC GAAGGCGAGA CCTTGCGAAG

101 CACCTTTCCC TCTTATATGG CCGAAGGCGA GCGGCTCTAC CTGTGCGGGG

151 AATTTTCTAA AGCCGCGCAG AGCTTCAGCA ACGCTCTTTA CCTTCAGGAT

201 GGAGACAAGA ACTGCCTGGT TGCTCGCTCA AAGTGCTTCC TGAAGATGGG

251 AGACTTGGAG AGATCCCTGA AGGATGCTGA GGCTTCGCTC CAGAGTGACC

301 CAGCTTTCTG TAAGGGGATT TTGCAAAAGG CTGAGACACT GTACACCATG

351 GGAGACTTTG AGTTTGCCTT GGTATTCTAT CATCGAGGCT ACAAGCTGAG

401 GCCTGATCGG GAATTCAGAG TTGGCATTCA GAAAGCCCAG GAAGCCATCA

451 ACAACTCAGT GGGAAGTCCT TCTTCCATTA AGCTGGAGAA CAAAGGGGAC

501 CTCTCCTTCT TAAGCAAGCA GGCTGAGAAT ATAAAAGCCC AGCAGAAGCC

551 TCAGCCCATG AAACACCTCT TACACCCCAC CAAGGGAGAG CCCAAGTGGA

601 AGGCCTCGCT CAAGAGTGAG AAGACTGTCC GCCAGCTTCT GGGGGAGCTC

651 TACGTGGACA AAGAGTATTT GGAGAAGCTC CTATTGGATG AAGACCTGAT

701 CAAAGGCACC ATGAAGGGCG GCCTGACTGT GGAGGACCTC ATCATGACGG

751 GCATCAACTA CCTGGATACT CACAGCAACT TCTGGAGGCA GCAGAAGCCG

801 ATCTACGCCA GGGAGCGGGA CCGGAAGCTG ATGCAAGAGA AATGGCTGCG

851 GGACCACAAA CGCCGTCCCT CACAGACAGC CCATTACATC CTCAAGAGCC

901 TGGAGGACAT TGATATGTTG CTCACAAGTG GCAGTGCTGA AGGGAGTCTT

951 CAGAAAGCTG AGAAAGTGCT GAAGAAGGTA CTGGAATGGA ACAAGGAAGA 1001 GGTACCCAAC AAGGATGAAC TGGTTGGAAA CTTGTATAGC TGCATAGGGA 1051 ATGCCCAGAT TGAGCTGGGG CAGATGGAGG CAGCCCTGCA GAGCCACAGA 1101 AAGGACCTGG AGATCGCCAA GGAATATGAC CTTCCTGATG CAAAATCGAG 1151 AGCCCTTGAC AACATTGGCA GAGTTTTTGC CAGAGTTGGG AAATTCCAGC 1201 AAGCCATTGA CACGTGGGAA GAAAAGATCC CTCTGGCAAA AACCACCCTG 1251 GAGAAGACCT GGCTGTTCCA CGAGATCGGC CGCTGCTACT TGGAGCTGGA 1301 CCAGGCCTGG CAGGCCCAGA ATTATGGCGA GAAGTCCCAG CAGTGTGCCG 1351 AGGAGGAAGG GGACATTGAG TGGCAACTGA ATGCCAGTGT TCTGGTGGCC 1401 CAGGCACAAG TGAAGCTGAG AGACTTCGAG TCAGCCGTGA ACAATTTTGA 1451 GAAGGCCCTG GAGAGAGCAA AGCTTGTGCA TAACAACGAG GCGCAGCAGG 1501 CCATCATCAG TGCCTTGGAC GATGCCAACA AGGGTATCAT CAGAGAACTG 1551 AGGAAAACCA ACTACGTGGA GAATCTCAAA GAAAAAAGCG AGGGAGAAGC 1601 TTCACTGTAT GAAGATAGAA TAATAACAAG AGAGAAGGAC ATGAGGAGAG 1651 TGAGAGATGA GCCCGAGAAG GTGGTGAAGC AGTGGGACCA TAGTGAGGAT 1701 GAGAAAGAGA CAGATGAGGA CGATGAGGCT TTTGGGGAAG CTCTGCAGAG 1751 CCCAGCAAGC GGAAAGCAGA GTGTGGAAGC AGGAAAAGCC AGAAGCGATT 1801 TGGGAGCAGT TGCCAAGGGC CTGTCAGGAG AATTAGGCAC AAGATCAGGA 1851 GAAACAGGCA GGAAGCTACT AGAAGCTGGC AGAAGAGAGT CAAGAGAAAT 1901 TTATAGGAGG CCTTCGGGAG AATTAGAGCA AAGACTCTCA GGAGAATTCA 1951 GCAGACAGGA ACCAGAAGAA CTAAAGAAAC TTTCAGAAGT GGGCAGAAGA 2001 GAGCCAGAAG AACTGGGAAA AACACAATTT GGAGAAATAG GAGAAACGAA 2051 AAAAACAGGA AATGAGATGG AAAAGGAATA TGAATGAAGC CATCGGTAGA 2101 GATGAGGATC AGGAAGCTGG TGTTCAGAGG GATCATGGGA TTTTATTAAA 2151 CTGGATTTTC AAGCGATTTG TCTGTTATAG GAAAAATGAG GGTTTTACTT 2201 CTGCTGCTTT CCATCACTAT TTTGCCATTA AATAGGTGTC TTTCACTCTT 2251 GCAAAAAAAA AAAAAAAAAA AAAAAAA

BLAST Results

No BLAST result Medline entries

No Medline entry

Peptide information for frame 3

ORF from 69 bp to 2084 bp; peptide length: 672 Category: similarity to known protein

1 MSDPEGETLR ΞTFPSYMAEG ERLYLCGEFS KAAQSFSNAL YLQDGDKNCL 51 VARSKCFLKM GDLERSLKDA EASLQSDPAF CKGILQKAET LYTMGDFEFA 101 LVFYHRGYKL RPDREFRVGI QKAQEAINNS VGSPSSIKLE NKGDLSFLSK 151 QAENIKAQQK PQPMKHLLHP TKGEPKWKAS LKSEKTVRQL LGELYVDKEY 201 LEKLLLDEDL IKGTMKGGLT VEDLIMTGIN YLDTHΞNFWR QQKPIYARER 251 DRKLMQEKWL RDHKRRPSQT AHYILKSLED IDMLLTSGSA EGSLQKAEKV 301 LKKVLEWNKE EVPNKDELVG NLYSCIGNAQ IELGQMEAAL QSHRKDLEIA 351 KEYDLPDAKS RALDNIGRVF ARVGKFQQAI DTWEEKIPLA KTTLEKTWLF 401 HEIGRCYLEL DQAWQAQNYG EKSQQCAEEE GDIEWQLNAS VLVAQAQVKL 451 RDFESAVNNF EKALERAKLV HNNEAQQAII SALDDANKGI IRELRKTNYV 501 ENLKEKSEGE AΞLYEDRIIT REKDMRRVRD EPEKVVKQWD HSEDEKETDE 551 DDEAFGEALQ SPASGKQSVE AGKARSDLGA VAKGLSGELG TRSGETGRKL 601 LEAGRRESRE IYRRPSGELE QRLSGEFSRQ EPEELKKLSE VGRREPEELG 651 KTQFGEIGET KKTGNEMEKE YE

BLASTP hits

Entry AF039202_1 from database TREMBL: product: "Hsp70/Hsp90 organizing protein"; Cricetulus griseus

Hsp70/Hsp90 organizing protein mRNA, complete eds.

Score = 149, P = 5.3e-07, identities = 42/160, positives = 74/160

Entry AI09782_1 from database TREMBL: product: "myosin heavy chain"; Argopecten irradians myosin heavy chain mRNA, complete eds.

Score = 155, P = 6. le-07, identities = 140/623, positives = 256/623

Entry S56658 from database PIR: stress-induced protein stil - soybean

Score = 156, P = 9.7e-08, identities = 41/153, positives = 72/153

Alert BLASTP hits for DKFZphtes3_15hl, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphtes3_15hl, frame 3

Report for DKFZphtes3_15hl .3

[LENGTH] 672

[MW] 76655.61

[pi] 5.49

[HOMOL] PIR:S56658 stress-induced protein stil - soybean 6e-10

[SUPFAM] tetratricopeptide repeat homology le-07

[PROSITE] MYRISTYL 7

[PROSITE] AMIDATION 3

[ PROSITE] CAMP_PHOSPHO_SITE 4

[PROSITE] CK2_PHOSPHO_SITE 15

[PROSITE] TYR_PHOSPHO_ΞITE 1

[PROSITE] PKC_PHOSPHO_SITE 11

[PROSITE] ASN_GLYCOSYLATION 2

[KW] All_Alpha

[KW] LOW_COMPLEXITY 4.76 %

SEQ MSDPEGETLRΞTFPSYMAEGERLYLCGEFSKAAQSFSNALYLQDGDKNCLVARSKCFLKM SEG PRD cccccccceeeccccccccccccccccchhhhhhhhhhhhhhccccceeehhhhhhhhhh

SEQ GDLERSLKDAEASLQSDPAFCKGILQKAETLYTMGDFEFALVFYHRGYKLRPDREFRVGI SEG PRD hcchhhhhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccchhhhhh SEQ QKAQEAINNSVGSPSSIKLENKGDLSFLSKQAENIKAQQKPQPMKHLLHPTKGEPKWKAS SEG PRD hhhhhhhhhhhhhhhhhhhhccchhhhhhhchhhhhhhcccchhhhhhcccccccchhhh

SEQ LKSEKTVRQLLGELYVDKEYLEKLLLDEDLIKGTMKGGLTVEDLIMTGINYLDTHSNFWR SEG xxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhccccccccccccc

SEQ QQKPIYARERDRKLMQEKWLRDHKRRPSQTAHYILKSLEDIDMLLTSGSAEGSLQKAEKV SEG PRD cchhhhhhhhhhhhhhhhhhhhccccccchhhhhhhhhhhheeeeeccccchhhhhhhhh

SEQ LKKVLEWNKEEVPNKDELVGNLYSCIGNAQIELGQMEAALQSHRKDLEIAKEYDLPDAKS SEG PRD hhhhhhhhcccccccceeecccccccchhhhhhhhhhhhhhhhhhhhhhhhhcccccchh

SEQ RALDNIGRVFARVGKFQQAIDTWEEKIPLAKTTLEKTWLFHEIGRCYLELDQAWQAQNYG SEG PRD hhhccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhh

SEQ EKSQQCAEEEGDIEWQLNAΞVLVAQAQVKLRDFESAVNNFEKALERAKLVHNNEAQQAII SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhh

SEQ SALDDANKGIIRELRKTNYVENLKEKSEGEASLYEDRIITREKDMRRVRDEPEKVVKQWD SEG x PRD hhhhccchhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhccceeeeecc

SEQ HSEDEKETDEDDEAFGEALQSPAΞGKQSVEAGKARSDLGAVAKGLSGELGTRSGETGRKL SEG xxxxxxxxxxxxx PRD ccccccccccchhhhhhhcccccccchhhhhccccccceeeeecccccccccccccchhh

SEQ LEAGRRESREIYRRPSGELEQRLSGEFSRQEPEELKKLSEVGRREPEELGKTQFGEIGET SEG PRD hhhcccccceeeeccccchhhhhcccccchhhhhhhhhhhcccccccccccccccccccc

SEQ KKTGNEMEKEYE SEG PRD cccccccccccc

Prosite for DKFZphtes3_15hl .3

PS00001 128->132 ASN_GLYCOSYLATION PDOC00001 PS00001 438->442 ASN_GLYCOSYLATION PDOC00001 PS00004 265->269 CAMP_PHOSPHO_SITE PDOC00004 PS00004 605->609 CAMP_PHOSPHO_SITE PDOC00004 PΞ00004 613->617 CAMP_PHOSPHO_SITE PDOC00004 PS00004 636->640 CAMP_PHOΞPHO_SITE PDOC00004 PS00005 8->ll PKC_PHOSPHO_SITE PDOC00005 PS00005 66->69 PKC_PHOSPHO_SITE PDOC00005 PS00005 136->139 PKC_PHOΞPHO_SITE PDOC00005 PS00005 180->183 PKC_PHOSPHO_SITE PDOC00005 PS00005 183->186 PKC_PHOSPHO_SITE PDOC00005 PS00005 186->189 PKC_PHOSPHO_SITE PDOC00005 PS00005 214->217 PKC_PHOSPHO_SITE PDOC00005 PS00005 342->345 PKC_PHOSPHO_SITE PDOC00005 PS00005 564->567 PKC_PHOΞPHO_SITE PDOC00005 PS00005 596->599 PKC_PHOSPHO_SITE PDOC00005 PS00005 660->663 PKC_PHOSPHO_SITE PDOC00005 PS00006 2->6 CK2_PHOSPHO_SITE PDOC00006 PS00006 66->70 CK2_PHOSPHO_SITE PDOC00006 PS00006 93->97 CK2_PHOSPHO_SITE PDOC00006 PS00006 171->175 CK2_PHOSPHO_SITE PDOC00006 PS00006 220->224 CK2_PHOSPHO_SITE PDOC00006 PS00006 277->281 CK2_PHOSPHO_SITE PDOC00006 PS00006 382->386 CK2_PHOSPHO_SITE PDOC00006 PS00006 392->396 CK2_PHOSPHO_SITE PDOC00006 PS00006 481->485 CK2_PHOSPHO_SITE PDOC00006 PS00006 507->511 CK2_PHOSPHO_SITE PDOC00006 PS00006 512->516 CK2_PHOSPHO_SITE PDOC00006 PS00006 542->546 CK2_PHOSPHO_SITE PDOC00006 PS00006 548->552 CK2_PHOSPHO_SITE PDOC00006 PS00006 628->632 CK2_PHOSPHO_SITE PDOC00006 PS00006 663->667 CK2_PHOSPHO_SITE PDOC00006 PS00007 506->515 TYR_PHOSPHO_SITE PDOC00007 PS00008 119->125 MYRISTYL PDOC00008 PS00008 132->138 MYRISTYL PDOC00008 PS00008 213->219 MYRISTYL PDOC00008 PS00008 288->294 MYRISTYL PDOC00008 PS00008 320->326 MYRISTYL PDOC00008 PS00008 334->340 MYRISTYL PDOC00008 PS00008 590->596 MYRISTYL PDOC00008 PS00009 596->600 AMIDATION PDOC00009 PS00009 603->607 AMIDATION PDOC00009 PS00009 641->645 AMIDATION PDOC00009

(No Pfam data available for DKFZphtes3_15hl .3)

DKFZphtes3_15ι5

group: cell structure and motility

DKFZphtes3_15ι5 encodes a novel 717 amino acid protein with similarity to radial spokehead proteins .

The novel protein is similar to the Chlamydomonas reinhardtn radial spokehead protein of flagella or axoneme and to the Strongylocentrotus purpuratus sea urchin spermatozoa protein p63. This protein is important for the maintenance of a planar form of sperm flagellar beating. In addition, the novel protein contains a transferrin signature 1 for iron-binding. The new protein seems to be a part of the human radial spoke heads in spermatozoa.

BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in modulating the structure of the human spermatozoa radia spoke head and modulation of sperm motility in men. strong similarity to "radial spokehead" proteins complete cDNA, complete eds, 1 EST hit (from a testis library) "radial spokehead" part of flagella in Chlamydomona, this protein seems to be part of the sperm motor or tail

Sequenced by GBF

Locus : unknown

Insert length: 2478 bp

Poly A stretch at pos. 2452, polyadenylation signal at pos. 2433

1 CACCCTGGCC CGCTCCCCGC GCCCTCCACG GGTAACGGCC CCCTCTCTCG 51 GTGCTCAGAA ACCGGCGGTG TCGACAGGTG GCTCTCGCTT GGCCTCCTTG

101 TCTGCAAGCC TTTCTCCTAG AGATCTGTGC CTCCTGGCGA ACCATGGGAG

151 ACCTGCCGCC CTACCCTGAG CGCCCTGCCC AGCAGCCTCC GGGCCGGAGG

201 ACTTCTCAGG CCTCCCAGAG GCGGCACAGT CGGGACCAAG CTCAGGCCCT

251 GGCAGCGGAC CCCGAGGAGA GGCAGCAGAT ACCTCCAGAC GCCCAGCGAA

301 ACGCCCCTGG TTGGTCACAG AGGGGCAGCC TGTCCCAACA GGAGAACTTG

351 CTGATGCCCC AGGTCTTCCA GGCTGAGGAA GCCCGGCTGG GTGGCATGGA

401 GTACCCATCT GTGAACACGG GCTTTCCCTC AGAGTTCCAG CCTCAGCCTT

451 ACTCTGATGA AAGCAGGATG CAGGTCGCCG AGCTCACCAC CAGCCTAATG

501 CTGCAGCGGC TCCAGCAGGG CCAAAGCAGC CTGTTCCAGC AACTGGACCC

551 CACCTTCCAG GAGCCCCCAG TCAACCCCTT GGGCCAGTTC AACCTCTACC

601 AGACAGACCA GTTCTCTGAA GGTGCCCAGC ACGGGCCTTA CATAAGGGAT

651 GACCCTGCCC TTCAGTTCTT GCCCTCTGAG CTGGGCTTCC CACACTACAG

701 TGCCCAGGTG CCTGAGCCCG AGCCTCTGGA GCTGGCCGTG CAGAACGCCA

751 AGGCCTACCT GCTGCAGACC AGCATCAATT GCGACCTCAG CCTGTACGAG

801 CACCTGGTAA ATCTGCTGAC CAAGATCCTG AACCAGCGGC CTGAGGACCC

851 CTTGTCTGTC CTGGAGTCTC TGAACCGCAC CACGCAGTGG GAGTGGTTCC

901 ACCCCAAGCT GGACACGCTG CGGGACGACC CCGAGATGCA GCCCACCTAC

951 AAGATGGCGG AGAAACAGAA GGCGCTGTTC ACCCGGAGTG GAGGCGGCAC 1001 TGAAGGCGAA CAGGAGATGG AGGAGGAGGT GGGGGAGACA CCAGTGCCCA 1051 ACATCATGGA GACTGCCTTC TACTTCGAGC AGGCCGGCGT CGGCCTGAGC 1101 TCGGACGAGA GCTTCCGCAT TTTCCTGGCC ATGAAACAGC TGGTGGAGCA 1151 GCAGCCCATC CACACCTGTC GCTTCTGGGG CAAGATCCTG GGAATCAAAC 1201 GCAGCTACCT GGTGGCCGAG GTGGAATTCC GGGAGGGCGA GGAGGAGGCA 1251 GAGGAGGAGG AGGTGGAGGA GATGACGGAA GGTGGCGAGG TCATGGAGGC 1301 GCACGGCGAG GAGGAGGGCG AGGAGGACGA GGAGAAGGCC GTGGACATCG 1351 TCCCTAAGTC CGTATGGAAG CCGCCGCCCG TGATCCCCAA GGAGGAGAGC 1401 CGCTCAGGCG CCAACAAGTA CCTGTACTTT GTGTGCAACG AGCCGGGCCT 1451 GCCATGGACG CGGCTGCCCC ACGTCACTCC AGCCCAGATC GTGAACGCCC 1501 GAAAGATCAA GAAGTTCTTC ACAGGCTACC TGGACACGCC AGTCGTCAGC 1551 TACCCACCCT TCCCGGGCAA CGAGGCCAAC TACCTGCGGG CCCAGATAGC 1601 CCGCATCTCG GCCGCCACGC AGGTCAGCCC GCTGGGCTTC TACCAGTTTA 1651 GTGAGGAGGA GGGCGACGAG GAGGAGGAAG GTGGTGCTGG GCGCGACTCC 1701 TACGAGGAGA ACCCGGACTT CGAGGGCATC CCCGTGCTGG AGCTGGTCGA 1751 CTCCATGGCC AACTGGGTGC ATCACACACA GCACATCCTG CCGCAGGGCC 1801 GCTGCACTTG GGTGAACCCT TTGCAGAAGA CAGAGGAGGA GGAGGACCTG 1851 GGGGAGGAGG AAGAGAAGGC AGATGAGGGG CCAGAGGAGG TGGAGCAGGA 1901 GGTTGGCCCC CCACTGCTAA CGCCACTTTC AGAAGATGCA GAAATCATGC 1951 ACCTGGCACC CTGGACCACC CGCCTGTCCT GCAGCCTCTG CCCGCAGTAC 2001 TCAGTGGCCG TTGTGCGCTC CAACCTCTGG CCCGGGGCCT ATGCCTATGC 2051 CAGTGGCAAA AAGTTTGAGA ACATCTACAT CGGCTGGGGT CACAAGTACA 2101 GCCCCGAGAG CTTCAACCCG GCCCTGCCAG CCCCCATTCA ACAAGAGTAC 2151 CCCAGTGGCC CAGAGATCAT GGAGATGAGT GACCCCACAG TGGAAGAGGA 2201 GCAGGCTCTG AAAGCAGCCC AGGAACAAGC CCTGGGAGCC ACAGAGGAGG 2251 AGGAGGAGGG CGAGGAGGAG GAGGAGGGCG AGGAGACAGA TGACTGAGGC 2301 CCACCCTCTA GCCACTTTCC CCAAGCAGGT AGATAGCAAA TTTCCCCTTA 2351 GAGGTAGTTA GCATGGATTA TATTTTCACT ATGTGCTTCC TGTCCCCAGA 2401 GGGCAGGGAT AGAAAAGGAA GGCAACTGCT TCAAATAAAA TTCCTCCACG 2451 GCATTAAAAA AAAAAAAAAA AAAAAAAG

BLAST Results

No BLAST result

Medline entries

86251010:

Molecular cloning and expression of flagellar radial spoke and dynein genes of

Chlamydomona

81142496:

Radial spokes of Chlamydomonas flagella: polypeptide composition and phosphorylation of stalk components.

9450971:

Molecular cloning and characterization of a radial spoke head protein of sea urchin sperm axonemes: involvement of the protein m the regulation of sperm motility.

Peptide information for frame 3

ORF from 144 bp to 2294 bp; peptide length: 717 Category: strong similarity to known protein

1 MGDLPPYPER PAQQPPGRRT SQASQRRHSR DQAQALAADP EERQQIPPDA

51 QRNAPGWSQR GSLSQQENLL MPQVFQAEEA RLGGMEYPSV NTGFPSEFQP

101 QPYSDESRMQ VAELTTSLML QRLQQGQSSL FQQLDPTFQE PPVNPLGQFN

151 LYQTDQFΞEG AQHGPYIRDD PALQFLPSEL GFPHYSAQVP EPEPLELAVQ

201 NAKAYLLQTS INCDLSLYEH LVNLLTKILN QRPEDPLSVL ESLNRTTQWE

251 WFHPKLDTLR DDPEMQPTYK MAEKQKALFT RSGGGTEGEQ EMEEEVGETP

301 VPNIMETAFY FEQAGVGLSS DESFRIFLAM KQLVEQQPIH TCRFWGKILG

351 IKRSYLVAEV EFREGEEEAE EEEVEEMTEG GEVMEAHGEE EGEEDEEKAV

401 DIVPKSVWKP PPVIPKEESR SGANKYLYFV CNEPGLPWTR LPHVTPAQIV

451 NARKIKKFFT GYLDTPVVSY PPFPGNEANY LRAQIARISA ATQVSPLGFY

501 QFSEEEGDEE EEGGAGRDSY EENPDFEGIP VLELVDSMAN WVHHTQHILP

551 QGRCTWVNPL QKTEEEEDLG EEEEKADEGP EEVEQEVGPP LLTPLSEDAE

601 IMHLAPWTTR LSCSLCPQYS VAVVRSNLWP GAYAYASGKK FENIYIGWGH

651 KYSPESFNPA LPAPIQQEYP SGPEIMEMSD PTVEEEQALK AAQEQALGAT

701 EEEEEGEEEE EGEETDD

BLASTP hits

Entry U73123_l from database TREMBL: product: "radial spokehead"; Strongylocentrotus purpuratus radial spokehead mRNA, complete eds .

Score = 1604, P = 7.4e-165, identities = 303/523, positives = 395/523

Entry B44498 from database PIR: radial spoke protein 6 - Chlamydomonas reinhardtn

Score = 386, P = 3.4e-45, identities = 105/264, positives 138/264

Alert BLASTP hits for DKFZphtes3_15ι5, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphtes3_15ι5, frame 3

Report for DKFZphtes3_15ι5.3

[LENGTH] 717

[MW] 80913.61

[pi] 4.36 [HOMOL] TREMBL:U73123_1 product: "radial spokehead"; Strongylocentrotus purpuratus radial spokehead mRNA, complete eds. le-130

[PROSITE] TRANSFERRING 1

[PROSITE] MYRISTYL 5

[PROSITE] AMIDATION 2

[PROSITE] CAMP_PHOSPHO_SITE 2

[PROSITE] CK2_PHOSPHO_SITE 14

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE

[PROSITE] ASN SLYCOSYLATION

[KW] All_Alpha

[KW] LOW COMPLEXITY 21.48 %

SEQ MGDLPPYPERPAQQPPGRRTSQASQRRHSRDQAQALAADPEERQQIPPDAQRNAPGWSQR

SEG .... xxxxxxxxxxxx

PRD ccccccccccccccccccccchhhhhhhhhhhhhhhhhcccccccccccccccccccccc

SEQ GSLSQQENLLMPQVFQAEEARLGGMEYPSVNTGFPSEFQPQPYSDESRMQVAELTTSLML

SEG xxxx

PRD cccchhhhhhhhhhhhhhhhhhccccccccccccccccccccccchhhhhhhhhhhhhhh

SEQ QRLQQGQSSLFQQLDPTFQEPPVNPLGQFNLYQTDQFSEGAQHGPYIRDDPALQFLPSEL

SEG xxxxxxxxxxxxxx

PRD hhhhhccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ GFPHYSAQVPEPEPLELAVQNAKAYLLQTSINCDLSLYEHLVNLLTKILNQRPEDPLSVL

SEG

PRD ccccccccccccccchhhhhhhhhhhhhhccccccchhhhhhhhhhhhhhhccccchhhh

SEQ ESLNRTTQWEWFHPKLDTLRDDPEMQPTYKMAEKQKALFTRSGGGTEGEQEMEEEVGETP

SEG xxxxxxxxxxxxxxxx ..

PRD hhhchhhhhccccccccccccccccchhhhhhhhhhhhhhhcccccchhhhhhhhhcccc

SEQ VPNIMETAFYFEQAGVGLSSDESFRIFLAMKQLVEQQPIHTCRFWGKILGIKRSYLVAEV

SEG xxx

PRD ccchhhhhhhhhhccccccchhhhhhhhhhhhhhhhhccchhhhhhhhcccchhhhhhhh

SEQ EFREGEEEAEEEEVEEMTEGGEVMEAHGEEEGEEDEEKAVDIVPKSVWKPPPVIPKEEΞR SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhcccccccccccccchhhhheeeeecccccccccccccccccc

SEQ SGANKYLYFVCNEPGLPWTRLPHVTPAQIVNARKIKKFFTGYLDTPVVSYPPFPGNEANY SEG PRD cccceeeeeeeccccccccccccccchhhhhhhhhhhhhhcccccccccccccccchhhh

SEQ LRAQIARISAATQVSPLGFYQFSEEEGDEEEEGGAGRDSYEENPDFEGIPVLELVDSMAN

SEG xxxxxxxxxxxxx

PRD hhhhhhhhhhhhccccccceeeeccccccccccccccccccccccccccceeeecchhhh

SEQ WVHHTQHILPQGRCTWVNPLQKTEEEEDLGEEEEKADEGPEEVEQEVGPPLLTPLSEDAE

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD hhhcccccccccceeechhhhhhhhhccccchhhhhcccccccccccccccccccccccc

SEQ IMHLAPWTTRLSCSLCPQYSVAVVRSNLWPGAYAYASGKKFENIYIGWGHKYSPESFNPA SEG PRD cccccccccccccccccccceeeeeeccccceeeecccccceeeeeeccccccccccccc

SEQ LPAPIQQEYPSGPEIMEMSDPTVEEEQALKAAQEQALGATEEEEEGEEEEEGEETDD

SEG xxxxxxxxxxxxxx ... xxxxxxxxxxxxxx ...

PRD cccccccccccccceeeeccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccc

Prosite for DKFZphtes3_15ι5.3

PS00001 244->248 ASN_GLYCOSYLATION PDOC00001 PS00002 282->286 GLYCOSAMINOGLYCAN PDOC00002 PS00004 18->22 CAMP_PHOSPHO_SITE PDOC00004 PS00004 26->30 CAMP_PHOSPHO_SITE PDOC00004 PS00005 24->27 PKC_PHOSPHO_SITE PDOC00005 PS00005 58->61 PKC_PHOSPHO_SITE PDOC00005 PS00005 258->261 PKC_PHOSPHO_SITE PDOC00005 PS00005 268->271 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 323->326 PKC_PHOSPHO_SITE PDOC00005 PS00005 341->344 PKC_PHOSPHO_SITE PDOC00005 PS00005 608->611 PKC_PHOSPHO_SITE PDOC00005 PS00005 637->640 PKC_PHOSPHO_SITE PDOC00005 PS00006 64->68 CK2_PHOSPHO_SITE PDOC00006 PS00006 137->141 CK2 PHOSPHO SITE PDOC00006 ?! ?: ?! ?: ?: to ro ro to ro to ro 1 1 1 1 1 1 en cn ω cn en ω en en 131313 K K K cn cn en en en en

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DKFZphtes3_15jl8

group: testes derived

DKFZphtes3_15j 18 encodes a novel 148 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, few EST hits

Sequenced by GBF

Locus : unknown

Insert length: 905 bp

Poly A stretch at pos. 839, polyadenylation signal at pos. 815

1 GTGATTCATA TGCTTCCATA GCAGGTGTCT GCTTCTGAGC CAAGCTCCCA

51 GGGCAGCGGA GCAGGCACCA ACCAGCATCC CAGGGGAGGG CACAGCTTGT

101 CCAGCTGGGA TGTTTGGGTG CCCTGTGAGA TGCCCCAAGC CACCAACCCA

151 GCTTATCTCA GGAGAAGCCT CGGCGGCCCG TCTGCCGGCC TGGAGAGATG

201 TGCTACAGCA GCCGGGGGTG GGGGGAGAGG GTGGGCTTAG AATCTCTTGG

251 CAGGGAGCCC CCAAGAGCAG GGTGAGACCT GCCTTCATTT CACCTGTCCC

301 CTTCACAGTT CTGCAAAGCC AGCATTATCA TCCCTTTTCA GAAGGAGTGG

351 GCACTCAGGT GGAATGCCTC ACCCCAGTCC TGCGGCTGGA AAGCGATATG

401 GCCAGGACTG CACCCCACCC CTCATCCCTG CACCCCTTCC CTGCCTGGGA

451 TTCCTCCAGC CCTGTGCACT GTGGAGCGCC TCTGCCTTCC GCTCATGGAG

501 GTTTCCCAAG GGCACGCGCT GAGGGCAGCT GGTCTCAGCC TGGGGCCGGG

551 TCCTAGTAAC TGTCTCTCTT TGCTTTCCAG CCAGTGTTTT GGGGTTTGAA

601 GTTGGAATCT TCAGCTACTG TCAAGAACAG CCACAAAAAT GTGTCACGAT

651 CAAGATCTTT GAGAGTCCAC CAATCAGGAG GCGTCTGTGA CAGTCGCTGT

701 CTTCTCAGAA CAGAATCCAC ACCCAGGATT CAACCCAAAT GATTTCTCAT

751 CAGGTGATTC TTGGTTGTAG CAAAGTTCAT GTGAATGTGG GTGAGTTTCT

801 GTTATGAATG TGGTCAATAA ATGTTATTTG TGAAACTCTA AAAAAAAAAA

851 AAAAAAAAAG GGCGGCCGCT CTAGAGGATC CAAGCTTACG TACGCGAAAA

901 AAAAG

BLAST Results

No BLAST result

Medlme entries

No Medline entry

Peptide information for frame 2

ORF from 110 bp to 553 bp; peptide length: 148 Category: putative protein

1 MFGCPVRCPK PPTQLISGEA SAARLPAWRD VLQQPGVGGE GGLRISWQGA 51 PKSRVRPAFI SPVPFTVLQS QHYHPFSEGV GTQVECLTPV LRLESDMART 101 APHPSSLHPF PAWDSSSPVH CGAPLPSAHG GFPRARAEGS WSQPGAGS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_15j 18, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphtes3_15 18, frame 2 Report for DKFZphtes3_15 18.2

[LENGTH] 148

[MW] 15665.78

[pl] 8.91

[PROSITE] MYRISTYL 3

[PROSITE] CK2_PHOSPHO_SITE

[KW] Irregular

SEQ MFGCPVRCPKPPTQLISGEASAARLPAWRDVLQQPGVGGEGGLRISWQGAPKSRVRPAFI PRD cccccccccccccccccccccccchhhhhhhccccccccccceeeeeccccccccccccc

SEQ SPVPFTVLQSQHYHPFSEGVGTQVECLTPVLRLESDMARTAPHPSSLHPFPAWDSSSPVH PRD cccceeeeeccccccccccccccccccchhhhhhhhcccccccccccccccccccccccc

SEQ CGAPLPSAHGGFPRARAEGSWSQPGAGS PRD cccccccccccccccccccccccccccc

Prosite for DKFZphtes3_15j 18.2

PS00006 82->86 CK2_PHOSPHO_SITE PDOC00006

PS00008 38->44 MYRISTYL PDOC00008

PS00008 42->48 MYRISTYL PDOC00008

PS00008 49->55 MYRISTYL PDOC00008

(No Pfam data available for DKFZphtes3_15j 18.2)

DKFZphtes3_15j3

group: nucleic acid management

DKFZphtes3_15 3 encodes a novel 743 amino acid protein with similarity to proteins with unknown function.

The novel protein contains a RNA recognition motif, predicted by Pfam and therefore binds to RNA. The protein is similar to YGR276c, a ribonuclease H of S. cerevisiae. Thus, the protein seems to a new RNA-modificating protein.

The new protein can find application in modulating the RNA metabolism in human cells and as a tool for biotechnologic manipulations .

"44M2.3"; product, differences to genmodel, similarity to ribonuclease H complete cDNA, complete eds, EST hits YGR276c = ribonuclease H differences to genmodel of 44M2.3

Sequenced by GBF

Locus: /map="16pll .2"

Insert length: 2695 bp

Poly A stretch at pos. 2601, polyadenylation signal at pos. 2579

1 GCGGTTGTTG TTGGCAGCTG TGGCTAAGGA GGGGAGAACC TCTGCTCCCC 51 GCCCGTCTTC TCTTCTGCGT TTCCCGGGCT AGGGGGCGTG GGGAGTGGTT

101 TTAGGCGGCG AAGCCGCTCG GCAGCACCTT CCTTCTTTGC CAGGCAGACG

151 CCCGTTGTAG CCGTTGGGGA ACCGTTGAGA ATCCGCCATG GAGCCAGAGA

201 GGGAAGGGAC CGAGAGACAC CCCAGGAAGG TCAGGGAAAG CAGGCAGGCC

251 CCAAATAAGC TGGTCGGGGC AGCTGAGGCG ATGAAAGCCG GTTGGGATCT

301 CGAGGAGAGT CAGCCCGAGG CCAAGAAAGC CCGCTTATCT ACCATTTTAT

351 TTACTGACAA CTGTGAAGTA ACCCATGACC AGCTGTGTGA ATTGCTGAAG

401 TATGCAGTTC TGGGCAAATC CAATGTTCCA AAACCCAGCT GGTGCCAGCT

451 TTTTCATCAA AACCACCTAA ACAACGTAGT GGTTTTTGTT CTGCAGGGAA

501 TGAGTCAGCT ACACTTTTAC AGGTTCTATT TGGAGTTTGG ATGTCTTCGA

551 AAAGCATTCA GACATAAATT CCGCTTGCCT CCACCATCAT CTGATTTTCT

601 AGCTGATGTT GTTGGGCTAC AAACTGAACA AAGAGCTGGA GATCTGCCCA

651 AGACAATGGA AGGGCCTTTA CCTTCTAATG CAAAAGCCGC CATCAACCTT

701 CAGGATGATC CCATCATTCA AAAGTATGGC TCTAAGAAAG TGGGCTTGAC

751 CAGATGCCTT CTGACAAAGG AGGAAATGAG AACGTTTCAC TTTCCATTAC

801 AAGGTTTTCC TGATTGTGAA AACTTTTTAC TTACCAAATG TAATGGTTCT

851 ATAGCAGACA ATAGTCCTCT CTTTGGACTT GACTGTGAAA TGTGCCTCAC

901 ATCCAAGGGG AGAGAGCTAA CACGCATCTC ACTGGTTGCT GAAGGAGGCT

951 GCTGTGTTAT GGATGAACTG GTCAAACCTG AAAACAAGAT TCTGGACTAC 1001 CTCACCAGCT TTTCGGGAAT CACGAAGAAG ATTCTTAACC CAGTGACGAC 1051 CAAACTCAAA GATGTACAGA GGCAGTTAAA AGCACTGCTT CCTCCTGATG 1101 CTGTGTTAGT GGGCCACTCC TTAGATTTGG ATCTCAGAGC ACTGAAAATG 1151 ATACATCCAT ATGTTATTGA TACATCGTTG CTTTATGTCA GAGAGCAGGG 1201 CAGAAGATTT AAGCTCAAGT TCTTAGCCAA AGTTATTTTG GGGAAGGATA 1251 TACAGTGTCC AGACAGACTT GGTCATGATG CCACAGAAGA TGCTAGAACA 1301 ATCCTTGAAT TGGCTCGGTA TTTCCTTAAG CATGGCCCAA AAAAGATTGC 1351 AGAACTAAAT CTAGAAGCAC TAGCTAATCA CCAAGAAATA CAAGCAGCAG 1401 GCCAAGAGCC TAAAAACACA GCAGAAGTAC TTCAGCACCC AAACACAAGT 1451 GTTTTAGAAT GCTTGGATTC AGTGGGTCAG AAGCTTCTTT TTTTGACCCG 1501 GGAGACAGAT GCTGGTGAAC TTCCATCTTC CAGAAATTGT CAAACTATTA 1551 AGTGTCTTTC AAATAAAGAG GTTCTTGAGC AGGCCAGAGT GGAAATCCCC 1601 CTGTTTCCCT TCAGCATTGT TCAGTTCTCT TTTAAGGCCT TTTCACCTGT 1651 CCTCACTGAG GAGATGAACA AAAGGATGAG GATCAAGTGG ACAGAGATAT 1701 CAACTGTCTA TGCTGGGCCA TTTAGCAAAA ATTGCAATCT CAGGGCTCTG 1751 AAGAGGCTGT TTAAAAGCTT TGGCCCAGTC CAGTCAATGA CTTTTGTTCT 1801 TGAAACCCGT CAGGTGCAGA GGCCTGTGAC AGAGCTCACG CTTGATTGTG 1851 ACACCCTCGT GAATGAGCTG GAAGGAGATT CTGAAAACCA AGGCTCTATA 1901 TATCTGTCTG GAGTGAGTGA AACCTTCAAA GAACAGCTAT TGCAGGAGCC 1951 CCGCCTCTTT CTTGGCCTGG AAGCTGTGAT CTTGCCTAAA GATCTTAAAA 2001 GTGGAAAGCA GAAAAAATAC TGTTTCCTGA AATTCAAAAG TTTTGGCAGT 2051 GCCCAGCAGG CCCTCAACAT TCTCACAGGC AAGGACTGGA AGCTGAAAGG 2101 CAGGCATGCC CTAACCCCCA GGCACCTCCA TGCCTGGCTC AGAGGCTTAC 2151 CACCTGAATC AACAAGGCTC CCAGGGCTTC GTGTTGTACC TCCCCCCTTT 2201 GAACAGGAGG CCTTGCAGAC TCTGAAACTG GACCACCCGA AGATAGCAGC 2251 CTGGCGCTGG AGCCGGAAGA TTGGAAAGCT CTACAACAGC TTGTGCCCGG 2301 GCACTCTCTG CCTCATCCTG CTGCCAGGAA CCAAGAGCAC TCATGGTTCA 2351 CTCTCTGGTC TAGGACTGAT GGGAATAAAA GAGGAAGAAG AAAGCGCTGG 2401 CCCAGGCCTG TGTTCGTGAG TCGGCCTGCC ATGTTTCCAT GTGCCATTTC 2451 TTACCCCTTG TAGGCAATGG CAAAGAATGT GGTCAGGCTG TAGCCTCCCC

2501 AACCAGCAGA CAGTTTTATG GAAACTTGGT ATAGCAGCTA AAAGAGTTTA

2551 GTTTGTTTAT ATGGCATGTA TAAGTTTTCA ATAAATGCCT AAAGTTCAAG

2601 CATAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

2651 AGGGCGGCCG CTCTAAAGGA TCCAAGCTTA CGTACGCGAA AAAAG

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 188 bp to 2416 bp; peptide length: 743 Category: similarity to known protein

1 MEPEREGTER HPRKVRESRQ APNKLVGAAE AMKAGWDLEE SQPEAKKARL

51 STILFTDNCE VTHDQLCELL KYAVLGKSNV PKPSWCQLFH QNHLNNVVVF

101 VLQGMSQLHF YRFYLEFGCL RKAFRHKFRL PPPSSDFLAD VVGLQTEQRA

151 GDLPKTMEGP LPSNAKAAIN LQDDPIIQKY GSKKVGLTRC LLTKEEMRTF

201 HFPLQGFPDC ENFLLTKCNG SIADNSPLFG LDCEMCLTSK GRELTRISLV

251 AEGGCCVMDE LVKPENKILD YLTSFSGITK KILNPVTTKL KDVQRQLKAL

301 LPPDAVLVGH SLDLDLRALK MIHPYVIDTS LLYVREQGRR FKLKFLAKVI

351 LGKDIQCPDR LGHDATEDAR TILELARYFL KHGPKKIAEL NLEALANHQE

401 IQAAGQEPKN TAEVLQHPNT SVLECLDSVG QKLLFLTRET DAGELPSSRN

451 CQTIKCLSNK EVLEQARVEI PLFPFSIVQF SFKAFSPVLT EEMNKRMRIK

501 WTEISTVYAG PFSKNCNLRA LKRLFKSFGP VQSMTFVLET RQVQRPVTEL

551 TLDCDTLVNE LEGDSENQGS IYLSGVSETF KEQLLQEPRL FLGLEAVILP

601 KDLKSGKQKK YCFLKFKSFG SAQQALNILT GKDWKLKGRH ALTPRHLHAW

651 LRGLPPESTR LPGLRVVPPP FEQEALQTLK LDHPKIAAWR WSRKIGKLYN

701 SLCPGTLCLI LLPGTKSTHG SLSGLGLMGI KEEEESAGPG LCS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_15j3, frame 2

TREMBL:AC004381_4 gene: "44M2.3"; product: "Unknown gene product"; Homo sapiens Chromosome 16 BAC clone CIT987SK-44M2, complete sequence., N = 2, Score = 1827, P = 2.1e-284

TREMBL:AF016430_4 gene: "C05C8.5"; Caenorhabditis elegans cosmid C05C8., N = 2, Score = 370, P = 1.7e-34

PIR:S64609 hypothetical protein YGR276c - yeast (Saccharomyces cerevisiae), N = 2, Score = 334, P = 1.8e-27

TREMBLNEW :SPAC637_9 gene: "SPAC637.09"; product: "putative exonuclease"; S. pombe chromosome I cosmid c637., N = 3, Score = 326, P = 2.8e-27

>TREMBL:AC004381_4 gene: "44M2.3"; product: "Unknown gene product"; Homo sapiens Chromosome 16 BAC clone CIT987SK-44M2, complete sequence. Length = 547

HSPs:

Score = 1827 (274.1 bits), Expect = 2.1e-284, Sum P(2) = 2.1e-284 Identities = 358/373 (95%), Positives = 358/373 (95%)

Query: 105 MSQLHFYRFYLEFGCLRKAFRHKFRLPPPSSDFLADVVGLQTEQRAGDLPKTMEGPLPSN 164

MSQLHFYRFYLEFGCLRKAFRHKFRLPPPSSDFLADVVGLQTEQRAGDLPKTMEGPLPΞN Sbjct: 1 MSQLHFYRFYLEFGCLRKAFRHKFRLPPPSSDFLADVVGLQTEQRAGDLPKTMEGPLPΞN 60

Query: 165 AKAAINLQDDPIIQKYGSKKVGLTRCLLTKEEMRTFHFPLQGFPDCENFLLTKCNGSIAD 224

AKAAINLQDDPIIQKYGSKKVGLTRCLLTKEEMRTFHFPLQGFPDCENFLLTKCNGSIAD Sbjct: 61 AKAAINLQDDPIIQKYGSKKVGLTRCLLTKEEMRTFHFPLQGFPDCENFLLTKCNGSIAD 120 Query: 225 NSPLFGLDCEM CLTSKGRELTRISLVAEGGCCVMDELVKPENKIL 269

NSPLFGLDCEM CLTSKGRELTRISLVAEGGCCVMDELVKPENKIL

Sbjct: 121 NSPLFGLDCEMARTTFNFSIGVLQAECLTSKGRELTRISLVAEGGCCVMDELVKPENKIL 180

Query: 270 DYLTSFSGITKKILNPVTTKLKDVQRQLKALLPPDAVLVGHSLDLDLRALKMIHPYVIDT 329 DYLTSFSGITKKILNPVTTKLKDVQRQLKALLPPDAVLVGHSLDLDLRALKMIHPYVIDT

Sbjct: 181 DYLTSFSGITKKILNPVTTKLKDVQRQLKALLPPDAVLVGHSLDLDLRALKMIHPYVIDT 240

Query: 330 SLLYVREQGRRFKLKFLAKVILGKDIQCPDRLGHDATEDARTILELARYFLKHGPKKIAE 389 SLLYVREQGRRFKLKFLAKVILGKDIQCPDRLGHDATEDARTILELARYFLKHGPKKIAE

Sbjct: 241 SLLYVREQGRRFKLKFLAKVILGKDIQCPDRLGHDATEDARTILELARYFLKHGPKKIAE 300

Query: 390 LNLEALANHQEIQAAGQEPKNTAEVLQHPNTSVLECLDΞVGQKLLFLTRETDAGELPSSR 449 LNLEALANHQEIQAAGQEPKNTAEVLQHPNTSVLECLDSVGQKLLFLTRETDAGELPSΞR

Sbjct: 301 LNLEALANHQEIQAAGQEPKNTAEVLQHPNTSVLECLDSVGQKLLFLTRETDAGELPSSR 360

Query: 450 NCQTIKCLSNKEV 462 NCQTIKCLSNKEV

Sbjct: 361 NCQTIKCLSNKEV 373

Score = 929 (139.4 bits), Expect = 2.1e-284, Sum P(2) = 2.1e-284

Identities ^:= 175/179 (97%), Positives = 177/179 (98%)

Query: 538 LETRQVQRPVTELTLDCDTLVNELEGDSENQGSIYLSGVSETFKEQLLQEPRLFLGLEAV 597 L ++VQRPVTELTLDCDTLVNELEGDSENQGSIYLSGVSETFKEQLLQEPRLFLGLEAV

Sbjct: 368 LSNKEVQRPVTELTLDCDTLVNELEGDSENQGSIYLSGVSETFKEQLLQEPRLFLGLEAV 427

Query: 598 ILPKDLKSGKQKKYCFLKFKSFGSAQQALNILTGKDWKLKGRHALTPRHLHAWLRGLPPE 657 ILPKDLKSGKQKKYCFLKFKSFGSAQQALNILTGKDWKLKGRHALTPRHLHAWLRGLPPE

Sbjct: 428 ILPKDLKSGKQKKYCFLKFKSFGSAQQALNILTGKDWKLKGRHALTPRHLHAWLRGLPPE 487

Query: 658 STRLPGLRVVPPPFEQEALQTLKLDHPKIAAWRWSRKIGKLYNSLCPGTLCLILLPGTK 716 STRLPGLRVVPPPFEQEALQTLKLDHPKIAAWRWSRKIGKLYNSLCPGTLCLILLPGTK

Sbjct: 488 STRLPGLRVVPPPFEQEALQTLKLDHPKIAAWRWSRKIGKLYNΞLCPGTLCLILLPGTK 546

Pedant information for DKFZphtes3_15j3, frame 2

Report for DKFZphtes3_15j3.2

[LENGTH] 743

[MW] 83536.58

[pi] 8.87

[HOMOL] TREMBL:AC004381_4 gene: "44M2.3"; product: "Unknown gene product"; Homo sapiens

Chromosome 16 BAC clone CIT987SK-44M2, complete sequence. 0.0

[FUNCAT] 01.03.16 polynucleotide degradation [S. cerevisiae, YGR276c] 4e-30

[ FUNCAT] 99 unclassified proteins [S. cerevisiae, YLR107w] 3e-13

[FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae,

YGL094C] le-10

[FUNCAT] 04.05.05 mrna processing (5'-end, 3'-end processing and mrna degradation) [S. cerevisiae, YGL094C] le-10

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YOL080C] 2e-10

[PROSITE] MYRISTYL 5

[PROSITE] AMIDATION 1

[PROSITE] CK2_PHOΞPHO_SITE 8

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE 16

(PROSITE] AΞN_GLYCOSYLATION 2

[PFAM] RNA recognition motif, (aka RRM, RBD, or RNP domain)

[KW] Alpha_Beta

SEQ MEPEREGTERHPRKVRESRQAPNKLVGAAEAMKAGWDLEESQPEAKKARLSTILFTDNCE PRD ccchhhhhccccchhhhhhhhcchhhhhhhhhhccccccccccchhhhhhccccccccce

SEQ VTHDQLCELLKYAVLGKSNVPKPSWCQLFHQNHLNNVVVFVLQGMSQLHFYRFYLEFGCL PRD eehhhhhhhhhhhhhcccccccccceeeeccccccceeeeeeecchhhhhhhhhhhhhhh

SEQ RKAFRHKFRLPPPΞSDFLADVVGLQTEQRAGDLPKTMEGPLPSNAKAAINLQDDPIIQKY PRD hhhhhhhhccccccccchhhhhhhhhhhhccccccccccccccchhhhhhhhcccccccc

SEQ GSKKVGLTRCLLTKEEMRTFHFPLQGFPDCENFLLTKCNGSIADNSPLFGLDCEMCLTSK PRD ccccccchhhhhhhhhhhhhhccccccccccceeeeccccccccccceeeeccccccccc

SEQ GRELTRISLVAEGGCCVMDELVKPENKILDYLTSFSGITKKILNPVTTKLKDVQRQLKAL PRD cchhhhheeeecccceeeeeeeccccceeecccccccccccccccccchhhhhhhhhhhh SEQ LPPDAVLVGHSLDLDLRALKMIHPYVIDTSLLYVREQGRRFKLKFLAKVILGKDIQCPDR PRD hccceeeecccchhhhhhhhhhhhccccceeeeccccccchhhhhhhhhhhhhhcccccc

SEQ LGHDATEDARTILELARYFLKHGPKKIAELNLEALANHQEIQAAGQEPKNTAEVLQHPNT PRD ccccchhhhhhhhhhhhhhhhcccceeeeehhhhhhhhhhhhhhccccccceeeeecccc

SEQ SVLECLDSVGQKLLFLTRETDAGELPSSRNCQTIKCLSNKEVLEQARVEIPLFPFSIVQF PRD ceeeeeeccccceeeeeecccccccccccccceeeeecchhhhhhhhhhccccccceeee

SEQ SFKAFSPVLTEEMNKRMRIKWTEISTVYAGPFSKNCNLRALKRLFKSFGPVQSMTFVLET PRD eeeceeeehhhhhhhhhhhhheeeeeecccccccchhhhhhhhhhhccccceeeehhhhh

SEQ RQVQRPVTELTLDCDTLVNELEGDSENQGSIYLSGVSETFKEQLLQEPRLFLGLEAVILP PRD cccccccccccccchhhhhhcccccccccccccccchhhhhhhhhhhhcccccceeeeec

SEQ KDLKSGKQKKYCFLKFKSFGSAQQALNILTGKDWKLKGRHALTPRHLHAWLRGLPPESTR PRD ccccccccceeeeeeeecccchhhhhhhhhccccccccccccccchhhhhhccccccccc

SEQ LPGLRVVPPPFEQEALQTLKLDHPKIAAWRWSRKIGKLYNSLCPGTLCLILLPGTKSTHG PRD ccccccccccchhhhhhhhhhcchhhhhhhhhhhhhheeeeccccceeeeeccccccccc

SEQ SLSGLGLMGIKEEEESAGPGLCS PRD cccccccchhhhhhccccccccc

Prosite for DKFZphtes3_15j3.2

PS00001 219->223 ASN_GLYCOSYLATION PDOC00001 PS00001 419->423 ASN_GLYCOSYLATION PDOC00001 PS00002 723->727 GLYCOSAMINOGLYCAN PDOC00002 PS00005 8->ll PKC_PHOSPHO_SITE PDOC00005 PS00005 182->185 PKC_PHOSPHO_SITE PDOC00005 PS00005 238->241 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 279->282 PKC_PHOΞPHO_SITE PDOC00005 PΞ00005 287->290 PKC_PHOSPHO_SITE PDOC00005 PS00005 447->450 PKC_PHOSPHO_SITE PDOC00005 PS00005 453->456 PKC_PHOSPHO_SITE PDOC00005 PS00005 458->461 PKC_PHOSPHO_SITE PDOC00005 PS00005 481->484 PKC_PHOSPHO_SITE PDOC00005 PS00005 579->582 PKC_PHOΞPHO_SITE PDOC00005 PS00005 605->608 PKC_PHOSPHO_SITE PDOC00005 PS00005 630->633 PKC_PHOSPHO_SITE PDOC00005 PS00005 643->646 PKC_PHOSPHO_SITE PDOC00005 PS00005 658->661 PKC_PHOΞPHO_SITE PDOC00005 PS00005 678->681 PKC_PHOSPHO_SITE PDOC00005 PS00005 692->695 PKC_PHOSPHO_SITE PDOC00005 PS00006 41->45 CK2_PHOSPHO_SITE PDOC00006 PS00006 193->197 CK2_PHOSPHO_SITE PDOC00006 PS00006 221->225 CK2_PHOSPHO_SITE PDOC00006 PS00006 371->375 CK2_PHOSPHO_SITE PDOC00006 PS00006 421->425 CK2_PHOSPHO_SITE PDOC00006 PS00006 458->462 CK2_PHOSPHO_SITE PDOC00006 PS00006 579->583 CK2_PHOSPHO_SITE PDOC00006 PS00006 630->634 CK2_PHOSPHO_SITE PDOC00006 PS00007 370->379 TYR_PHOSPHO_SITE PDOC00007 PS00008 27->33 MYRISTYL PDOC00008 PS00008 186->192 MYRISTYL PDOC00008 PS00008 575->581 MYRISTYL PDOC00008 PS00008 714->720 MYRISTYL PDOC00008 PS00008 720->726 MYRISTYL PDOC00008 PS00009 337->341 AMIDATION PDOC00009

Pfam for DKFZphtes3_15j3.2

HMM_NAME RNA recognition motif, (aka RRM, RBD, or RNP domain)

HMM *IYVGNLPWDtTEEDLrDlFsQFGpIvsIrMMrDReTGRSRGFAFVEFED

IY+ +++ +T +E+L + + F + + + +++D G+ + ++F +F++

Query 571 IYLSGVS-ETFKEQLLQEPRLFLGLEAVILPKDLKSGKQKKYCFLKFKS 618

HMM EEDAekAIdeMNG..meFmGRrlRV*

+A+ A+ + G ++ GR + Query 619 FGSAQQALNILTGKDWKLKGRHALT 643 DKFZphtes3 15kll

group: signal transduction

DKFZphtes3_15kll encodes a novel 958 amino acid protein C-terminal identical with human KIAA0781 protein and high similarity to protein kinases.

The novel protein contains a protein kinase ATP-binding region signature and a serine/threomne protein kinase active-site signature. The related murine kinase was cloned from the myocardium of the developing heart.

The new protein can find application m modulation of intracellular signal pathways dependent on this kinase.

KIAA0781, 5' extension complete cDNA, complete eds, potential start at Bp 97, EST hits

Sequenced by GBF

Locus: /map="ll"

Insert length: 4868 bp

Poly A stretch at pos. 4798, polyadenylation signal at pos. 4776

1 GAGCAAGCGG AGCGGCCGTC GCCCAAGCCA AGCCGCGCTG CCAACCCTCC

51 CGCCCGCCCG CGCTCCTGTC CGCCGTGTCT AGCAGCGGGG CCCAGCATGG

101 TCATGGCGGA TGGCCCGAGG CACTTGCAGC GCGGGCCGGT CCGGGTGGGG

151 TTCTACGACA TCGAGGGCAC GCTGGGCAAG GGCAACTTCG CTGTGGTGAA

201 GCTGGGGCGG CACCGGATCA CCAAGACGGA GGTGGCAATA AAAATAATCG

251 ATAAGTCTCA GCTGGATGCA GTGAACCTTG AGAAAATCTA CCGAGAAGTA

301 CAAATAATGA AAATGTTAGA CCACCCTCAC ATAATCAAAC TTTATCAGGT

351 AATGGAGACC AAAAGTATGT TGTACCTTGT GACAGAATAT GCCAAAAATG

401 GAGAAATTTT TGACTATCTT GCTAATCATG GCCGGTTAAA TGAGTCTGAA

451 GCCAGGCGAA AATTCTGGCA AATCCTGTCT GCTGTTGATT ATTGTCATGG

501 TCGGAAGATT GTGCACCGTG ACCTCAAAGC TGAAAATCTC CTGCTGGATA

551 ACAACATGAA TATCAAAATA GCAGATTTCG GTTTTGGAAA TTTCTTTAAA

601 AGTGGTGAAC TGCTGGCAAC ATGGTGTGGC AGCCCCCCTT ATGCAGCCCC

651 AGAAGTCTTT GAAGGGCAGC AGTATGAAGG ACCACAGCTG GACATCTGGA

701 GTATGGGAGT TGTTCTTTAT GTCCTTGTCT GTGGAGCTCT GCCCTTTGAT

751 GGACCGACTC TTCCAATTTT GAGGCAGAGG GTTCTGGAAG GAAGATTCCG

801 GATTCCGTAT TTCATGTCAG AAGATTGCGA GCACCTTATC CGAAGGATGT

851 TGGTCCTAGA CCCATCCAAA CGGCTAACCA TAGCCCAAAT CAAGGAGCAT

901 AAATGGATGC TCATAGAAGT TCCTGTCCAG AGACCTGTTC TCTATCCACA

951 AGAGCAAGAA AATGAGCCAT CCATCGGGGA GTTTAATGAG CAGGTTCTGC

1001 GACTGATGCA CAGCCTTGGA ATAGATCAGC AGAAAACCAT TGAGTCTTTG

1051 CAGAACAAGA GCTATAACCA CTTTGCTGCC ATTTATTTCT TGTTGGTGGA

1101 GCGCCTGAAA TCACATCGGA GCAGTTTCCC AGTGGAGCAG AGACTTGATG

1151 GCCGCCAGCG TCGGCCTAGC ACCATTGCTG AGCAAACAGT TGCCAAGGCA

1201 CAGACTGTGG GGCTCCCAGT GACCATGCAT TCACCGAACA TGAGGCTGCT

1251 GCGATCTGCC CTCCTCCCCC AGGCATCCAA CGTGGAGGCC TTTTCATTTC

1301 CAGCATCTGG CTGTCAGGCG GAAGCTGCAT TCATGGAAGA AGAGTGTGTG

1351 GACACTCCAA AGGTCAATGG CTGTCTGCTT GACCCTGTGC CTCCTGTCCT

1401 GGTGCGGAAG GGATGCCAGT CACTGCCCAG CAACATGATG GAGACCTCCA

1451 TTGACGAAGG GCTGGAGACA GAAGGAGAGG CCGAGGAAGA CCCCGCTCAT

1501 GCCTTTGAGG CATTTCAGTC CACACGCAGC GGGCAGAGAC GGCACACTCT

1551 GTCAGAAGTG ACCAATCAAC TGGTCGTGAT GCCTGGGGCA GGGAAAATTT

1601 TCTCCATGAA TGACAGCCCC TCCCTTGACA GTGTGGACTC TGAGTATGAT

1651 ATGGGGTCTG TTCAGAGGGA CCTGAACTTT CTGGAAGACA ACCCTTCCCT

1701 TAAGGACATC ATGTTAGCCA ATCAGCCTTC ACCCCGCATG ACATCTCCCT

1751 TCATAAGCCT GAGACCTACC AACCCAGCCA TGCAGGCTCT GAGCTCCCAG

1801 AAACGAGAGG TCCACAACAG GTCTCCAGTG AGCTTCAGAG AGGGCCGCAG

1851 AGCATCAGAT ACCTCCCTCA CCCAGGGAAT TGTAGCATTT AGACAACATC

1901 TTCAGAATCT GGCTAGAACC AAAGGAATTC TAGAGTTGAA CAAAGTGCAG

1951 TTGTTGTATG AACAAATAGG ACCGGAGGCA GACCCTAACC TGGCGCCGGC

2001 GGCTCCTCAG CTCCAGGACC TTGCTAGCAG CTGCCCTCAG GAAGAAGTTT

2051 CTCAGCAGCA GGAAAGCGTC TCCACTCTCC CTGCCAGCGT GCATCCCCAG

2101 CTGTCCCCAC GGCAGAGCCT GGAGACCCAG TACCTGCAGC ACAGACTCCA

2151 GAAGCCCAGC CTTCTGTCAA AGGCCCAGAA CACCTGTCAG CTTTATTGCA

2201 AAGAACCACC GCGGAGCCTT GAGCAGCAGC TGCAGGAACA TAGGCTCCAG

2251 CAGAAGCGAC TCTTTCTTCA GAAGCAGTCT CAACTGCAGG CCTATTTTAA

2301 TCAGATGCAG ATAGCAGAGA GCTCCTACCC ACAGCCAAGT CAGCAGCTGC

2351 CCCTTCCCCG CCAGGAGACT CCACCGCCTT CTCAGCAGGC CCCACCGTTC

2401 AGCCTGACCC AGCCCCTGAG CCCCGTCCTG GAGCCTTCCT CCGAGCAGAT

2451 GCAATACAGC CCTTTCCTCA GCCAGTACCA AGAGATGCAG CTTCAGCCCC

2501 TGCCCTCCAC TTCCGGTCCC CGGGCTGCTC CTCCTCTGCC CACGCAGCTA

2551 CAGCAGCAGC AGCCGCCACC GCCACCACCC CCTCCACCAC CACGACAGCC

2601 AGGAGCTGCC CCAGCCCCCT TACAGTTCTC CTATCAGACT TGTGAGCTGC 2651 CAAGCGCTGC TTCCCCTGCG CCAGACTATC CCACTCCCTG TCAGTATCCT

2701 GTGGATGGAG CCCAGCAGAG CGACCTAACG GGGCCAGACT GTCCCAGAAG

2751 CCCAGGACTG CAAGAGGCCC CCTCCAGCTA CGACCCACTA GCCCTCTCTG

2801 AGCTACCTGG ACTCTTTGAT TGTGAAATGC TAGACGCTGT GGATCCACAA

2851 CACAACGGGT ATGTCCTGGT GAATTAGTCT CAGCACAGGA ATTGAGGTGG

2901 GTCAGGTGAA GGAAGAGTGT ATGTTCCTAT TTTTATTCCA GCCTTTTAAA

2951 TTTAAAGCTT ATTTTCTTGC CCTCTCCCTA ACGGGGAGAA ATCGAGCCAC

3001 CCAACTGGAA TCAGAGGGTC TGGCTGGGGT GGATGTTGCT TCCTCCTGGT

3051 TCTGCCCCAC CACAAAGTTT TCTGTGGCAA GTGCTGGAAC ATAGTTGTAG

3101 GCTGAGGCTC CTGCCCTTCG GTCGAGTGGA GCAAGCTCTC GAGGGCAGCA

3151 CTGACAAATG TGTTCCTAAG AAGACATTCA GACCCAGGTC TTATGCAGGA

3201 TTACATCCGT TTATTATCAA GGGCAACCTT GGTGAAAGCA GAAAGGGTGT

3251 GTGCTATTGC ATATATATGG GGGAAAAGGC AATATATTTT TCACTGAAGC

3301 TGAGCAACCA CATATTGCTA CAAGGCAAAT CAAGAAGACA TCAGGAAATC

3351 AGATGCACAG GAAATAAAGG AAAGCTGTGC TTTGTCATTG AATCCTAAGT

3401 TCTTAGCTGC TGATGCAAGT TGTCCCCCAA GGCCATCACA AAGCAGTGGG

3451 GCATGAGCTG TGTTTCAGGG GCCACTAAAT AACAGCTGGT ACTGACCCCA

3501 GAAACCGCCT TCATCTCCAT TCGGAAGCAG GTGACACACC CCTTCAGAAG

3551 GTGCCCTGGG TTGCCGAGTG TCAGAATATA CTCAGGACTC CAGAGGTGTC

3601 ACACGTGGAA CTGACAGGAG ACCCGCCACC GTGGAGGCAG GGGGCAAGAA

3651 ACTCAAGAAC GCATCAAGAG CACCAGCCCT GGGCCAGGGA AGACAGGCTC

3701 TTCCTGCAGT TTCTCGTGGA CACTGCTGGC TTGCGGGCAG TCGGTCTCCA

3751 GGGTACCTGT TGTCTCTTTT CCGATGTAAT AACTACTTTG ACCTTACACT

3801 ATATGTTGCT AGTAGTTTAT TGAGCTTTGT ATATTTGGAC AGTTTCATAT

3851 AGGGCTTAGA GATTTTAAGG ACATGATAAA TGAACTTTTC TGTCCCATGT

3901 GAAGTGGTAG TGCGGTGCCT TTCCCCCAGA TCATGCTTTA ATTCTTTCTT

3951 TTCTGTAGAA ACCAACAGTT TCCATTTATG TCAATGCTAA ATCCAAAGTC

4001 ACTTCAGAGT TTGTTTTCCA CCATGTGGGA ATCAGCATTC TTAATTTCGT

4051 TAAAGTTTTG ACTTGTAATG AAATGTTCAA GTATTACAGC AATATTCAAA

4101 GAAAGAACCA CAGATGTGTT AACCATTTAA GCAGATCATC TGCCAAACAT

4151 TATATTACTA ATAAAACTTA ACCAACACTT ACAATTCAGT CATCAAAGTA

4201 AGTAAAAATT AGATGCTACA GCTAGCTAAC TGTATCCCTA GAAATGATGA

4251 ATAATTTGCC ATTTGGACAG TTAACATCCA GGTGTTACAA AGTCAGTGTT

4301 AATTCTAAAG ATGATCATTT CTGCCCTTTA GAATGGCTTG TCCCATCAGC

4351 AGATGAATGT GTTAAGCACA AAGCATCTTC CTTAAAGCAC AAAGAGAGGG

4401 ACTAACTGAT GCTGCATCTA GAAAACACCT TTAAGTTGCC TTTCCTCTTT

4451 GTAGTTAGCG TTCAGGCAGG TGACGTGTGG AAAGTCTAGG GGGTTCCATT

4501 CTGGCCATGC GAGCCCAGCT CCTACCAACG TCGGTAACTT GAGCAGTCCC

4551 TGTTGCTGGC CAGAGACTGC CTGGTCGCCA GCGCTCACCA TGGGTGCCAG

4601 GATGCTTCGC AGAGGCACTG TGCTCACGGT TGGACTTGGT GTCAGTGGGA

4651 AAGGGCAGTG TGGGGACTGT CATTTTTGTG ATTTAATAAC ACACAGTGAA

4701 AATCCAGGAA GAATGAATTA AGCTTCTTCT GGGAGTTGTT TATTCCTGCT

4751 CGTGCTTAAG ATTGATGATT TCGTGAAATA AAGAACATCA TTTCATTTAA

4801 AAAAAAAAAA AAAAAAAGGG CGGCCGCTCT AGAGGATCCA AGCTTACGTA

4851 CGCGTGAAAA AAAAAAAG

BLAST Results

Entry HSG4921 from database EMBL: human STS SHGC-37164. Score = 1605, P = 1.9e-66, identities = 349/369

Entry AB018324 from database EMBL:

Homo sapiens mRNA for KIAA0781 protein, partial eds.

Score = 10725, P = O.Oe+00, identities = 2145/2145

Medline entries

No Medline entry

Peptide information for frame 1

ORF from the beginning to 2874 bp; peptide length: 959 Category: known protein

1 EQAERPSPKP SRAANPPARP RSCPPCLAAG PSMVMADGPR HLQRGPVRVG 51 FYDIEGTLGK GNFAVVKLGR HRITKTEVAI KIIDKSQLDA VNLEKIYREV 101 QIMKMLDHPH IIKLYQVMET KSMLYLVTEY AKNGEIFDYL ANHGRLNESE 151 ARRKFWQILS AVDYCHGRKI VHRDLKAENL LLDNNMNIKI ADFGFGNFFK 201 SGELLATWCG SPPYAAPEVF EGQQYEGPQL DIWSMGVVLY VLVCGALPFD 251 GPTLPILRQR VLEGRFRIPY FMSEDCEHLI RRMLVLDPSK RLTIAQIKEH 301 KWMLIEVPVQ RPVLYPQEQE NEPSIGEFNE QVLRLMHSLG IDQQKTIESL 351 QNKSYNHFAA IYFLLVERLK SHRSSFPVEQ RLDGRQRRPS TIAEQTVAKA 401 QTVGLPVTMH SPNMRLLRSA LLPQASNVEA FSFPASGCQA EAAFMEEECV 451 DTPKVNGCLL DPVPPVLVRK GCQSLPSNMM ETSIDEGLET EGEAEEDPAH 501 AFEAFQSTRS GQRRHTLSEV TNQLVVMPGA GKIFSMNDSP SLDSVDSEYD 551 MGSVQRDLNF LEDNPSLKDI MLANQPSPRM TSPFISLRPT NPAMQALSSQ 601 KREVHNRΞPV SFREGRRASD TSLTQGIVAF RQHLQNLART KGILELNKVQ 651 LLYEQIGPEA DPNLAPAAPQ LQDLASSCPQ EEVSQQQESV STLPASVHPQ 701 LSPRQSLETQ YLQHRLQKPS LLSKAQNTCQ LYCKEPPRSL EQQLQEHRLQ 751 QKRLFLQKQS QLQAYFNQMQ IAESSYPQPΞ QQLPLPRQET PPPSQQAPPF 801 SLTQPLSPVL EPSSEQMQYS PFLSQYQEMQ LQPLPSTSGP RAAPPLPTQL 851 QQQQPPPPPP PPPPRQPGAA PAPLQFSYQT CELPSAASPA PDYPTPCQYP 901 VDGAQQSDLT GPDCPRSPGL QEAPSSYDPL ALSELPGLFD CEMLDAVDPQ 951 HNGYVLVN

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_15kll, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphtes3_15kll, frame 1

Report for DKFZphtes3_15kll .1

[LENGTH] 926 [MW] 103915.77 [pi] 5.70 [HOMOL] TREMBL:AB018324_1 gene: "KIAA0781"; product: "KIAA0781 protein"; Homo sapiens mRNA for KIAA0781 protein, partial eds. 0.0

[FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YDR477w]

8e-76

[FUNCAT] 11.01 stress response [S. cerevisiae, YDR477w] 8e-76

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDR477w] 8e-76

[FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YCL024w] 4e-58

[FUNCAT] 03.25 cytokinesis [S. cerevisiae, YDR507c] 3e-56

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YDR507c]

3e-56

[FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YDR122w] le-53

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YKLlOlw] 3e-53

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKLlOlw] 3e-53

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YPL141c] 5e-51

[FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YPL153c] 3e-42

[FUNCAT] 03.22.01 cell cycle check point proteins [S. cerevisiae, YPL153c] 3e-42

[FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YPL153c] 3e-42

[FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YPL153c] 3e-42

[FUNCAT] 03.01 cell growth [S. cerevisiae, YFR014c] 5e-42

[FUNCAT] 03.16 dna synthesis and replication [S. cerevisiae, YMROOlc] 2e-34

[FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YGL180w] le-27

[FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YGL180w] le-27

[FUNCAT] 06.13.04 lysosomal and vacuolar degradation [S. cerevisiae, YGL180w] le-27

[FUNCAT] 10.02.11 key kinases [S. cerevisiae, YBL105c] 3e-26

[FUNCAT] 04.99 other transcription activities [S. cerevisiae, YER129w] 3e-26

[FUNCAT] 02.19 metabolism of energy reserves (glycogen, trehalose) [S. cerevisiae,

YPL031C] le-23

[ FUNCAT] 01 04.04 regulation of phosphate utilization [S. cerevisiae, YPL031c] le-23

[FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YPL031C] le-23

[FUNCAT] 03.13 meiosis [S. cerevisiae, YOR351c] 2e-23

[FUNCAT] 10.05.11 key kinases [S. cerevisiae, YHL007c] 8e-21

[ FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [S. cerevisiae, YHL007c] 8e-21

[FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YPL140c] 2e-20

[FUNCAT] 10.03.11 key kinases [S. cerevisiae, YLR113w] le-20

[FUNCAT] 04.05.01.01 general transcription activities [S. cerevisiae, YDL108w] 3e-19

[FUNCAT] 10.05.09 regulation of g-protem activity [S. cerevisiae, YBLOlδw] 2e-18

[FUNCAT] 10.04.11 key kinases [S. cerevisiae, YLR362w] 3e-18

[FUNCAT] 04.03.99 other trna-transcription activities [S. cerevisiae, YOR061w] 4e-18

[FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YFL033c] 4e-17

[FUNCAT] 05.07 translational control [S. cerevisiae, YDR283c] 2e-16

[FUNCAT] 01.02.04 regulation of nitrogen and sulphur utilization [S. cerevisiae, YNL183c] 2e-14 [FUNCAT] 08.99 other intracellular-transport activities [S. cerevisiae, YNL183c]

2e-14

[FUNCAT] 09.04 biogenesis of cytoskeleton [S. cerevisiae, YNL020C] 5e-14

[FUNCAT] c energy conversion [M. gemtalium, MG109] 2e-12

[FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae,

YBR097w) le -10

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YBR097w] le-10

[FUNCAT] 30.08 organization of golgi [S. cerevisiae, YBR097w] le-10

[FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YBR097w] le-10

[FUNCAT] 10.04.99 other nutritional-response activities [S. cerevisiae, YJR059w]

4e-09

[FUNCAT] 01.06.10 regulation of lipid, fatty-acid and sterol biosynthesis [S. cerevisiae YHR079c] le-07

[FUNCAT] 30.07 organization of endoplasmatic reticulum [S. cerevisiae, YHR079c] le-07

[FUNCAT] 08.19 cellular import [S. cerevisiae, YNL154c] 2e-04

[BLOCKS] BL00415A Synapsins proteins

[BLOCKS] BL00239B Receptor tyrosine kinase class II proteins

[BLOCKS] BL00107A Protein kinases ATP-binding region proteins

[SCOP] dlgol 1.1.1. MAP kinase Erk2 [rat Rattus norvegicus 3e-78

[SCOP] dlwfc 1.1.1.8 MAP kinase p38 [human (Homo sapiens) le-81

[SCOP] dlkoa_2 1.1.1.7 (1-350) Twitchm, kinase domain [Caenorhabditi 5e-89

[SCOP] dlkoba_ 1.1. .1.6 Twitchm, kinase domain [California sea har 5e-86

[SCOP] dlphk 1.1..1.5 gamma-subumt of glycogen phosphorylase kinas 3e-80

[SCOP] dlirk 1.1.2.4 insulin receptor [Human (Homo sapiens) 6e-70

[SCOP] dlapme_ 1.1.1.4 cAMP-dependent PK, catalytic subunit [mouse (Mu le-95

[SCOP] dlfgka_ 1.1.2.3 Fibroblast growth factor receptor 1 [human (Hom 7e-71

[SCOP] dlydse_ 1.1.1.3 cAMP-dependent PK, catalytic subunit [bovine (Bo 2e-96

[SCOP] dlfmk_3 1.1.2.2 (168-437) c-src tyrosine kinase [human (Hom 2e-72

[SCOP] dlcdka_ 1.1.1.2 cAMP-dependent PK, catalytic subunit [pig (Su 5e-97

[SCOP] d2hckb3 1.1.2.1 (167-437) Haemopoetic cell kinase Hck [huma 2e-68

[SCOP] dlcsn 1.1.1.11 Casein kmase-1, CK1 [Schizosaccharomyces pombe 3e-53

[SCOP] dl sua_ 1.1.1.1 Cyclin-dependent PK [Human (Homo sapiens) 3e-78

[SCOP] dlckιa__ 1.1.1.10 Casein kmase-1, CKl [rat (Rattus norvegicus) le-58

[EC] 2.7.1.117 Myosin-l ght-chain kinase 3e-49

[EC] 2. 7.1.109 [Hydroxymethylglutaryl-CoA reductase (NADPH) ] kinase 4e-78

[EC] 2 7.1.38 Phosphorylase kinase 3e-41

[EC] 2 7.1.37 Protein kinase 7e-45

[EC] 2 7.1.123 Ca2+/calmodulιn-dependent protein kinase 5e-42

[EC] 2 7.1.128 [Acetyl-CoA carboxylase] kinase 4e-78

[PIRKW] phosphotransferase 3e-93

[PIRKW] nucleus 2e-74 '

[PIRKW] calcium 2e-40

[PIRKW] transferase 3e-33

[PIRKW] duplication 2e-32

[PIRKW] tandem repeat 7e-45

[PIRKW] phorbol ester binding 4e-33

[PIRKW] zinc 4e-33

[PIRKW] ion transport le-32

[PIRKW] cell cycle control le-45

[PIRKW] seπne/threonme-specific protein kmase 2e-97

[PIRKW] oncogene le-34

[PIRKW] phospholipid binding 2e-32

[PIRKW] autophosphorylation 2e-74

[PIRKW] brain 6e-36

[PIRKW] heterotetramer 8e-38

[PIRKW] mitosis le-45

[PIRKW] polymer 5e-41

[PIRKW] magnesium 6e-80

[PIRKW] ATP 2e-97

[PIRKW] polyprotein le-34

[PIRKW] alternative initiators 2e-31

[PIRKW] phosphoprotein 2e-74

[PIRKW] apoptosis 8e-38

[PIRKW] cGMP binding 4e-33

[PIRKW] glycoprotein 3e-36

[PIRKW] skeletal muscle 8e-38

[PIRKW] protein kinase 2e-50

[PIRKW] testis 5e-41

[PIRKW] cAMP binding 8e-38

[PIRKW] transforming protein 4e-33

[PIRKW] purme nucleotide binding 7e-52

[PIRKW] calcium binding 7e-45

[PIRKW] alternative splicing 5e-42

[PIRKW] P-loop 7e-52

[PIRKW] lipoprotein 8e-38

[PIRKW] proto-oncogene 4e-33

[PIRKW] segmentation le-34

[PIRKW] core protein le-34 [PIRKW] muscle 8e-38

[PIRKW] myristylation 8e-38

[PIRKW] EF hand 7e-45

[PIRKW] cell division 3e-49

[PIRKW] homodimer le-32

[PIRKW] calmodulin binding 5e-42

[SUPFAM] ribosomal protein S6 kinase II le-34

[SUPFAM] calcium-dependent protein kinase 7e-45

[SUPFAM] AMP-activated protein kinase 6e-80

[SUPFAM] protein kmase akt 3e-36

[SUPFAM] protein kinase SPK1 7e-41

[SUPFAM] unassigned Ser/Thr or Tyr-specific protein kinases 8e-99

[SUPFAM] Ca2+/calmodulιn-dependent protein kinase 5e-42

[SUPFAM] calmodulin repeat homology 7e-45

[SUPFAM] cAMP receptor protein cyclic nucleotide-bmding domain homology 3e-33

[SUPFAM] protein kmase DUN1 6e-36

[SUPFAM] protein kinase C zeta 4e-33

[SUPFAM] Dictyostelium cAMP-dependent protein kinase catalytic chain 2e-34

[SUPFAM] death-associated protein kinase 8e-38

[SUPFAM] pleckstrm repeat homology 3e-36

[SUPFAM] ankyrin repeat homology 8e-38

[SUPFAM] protein kinase homology 8e-99

[SUPFAM] Ca2+/calmodulιn-dependent protein kinase II 6e-38

[SUPFAM] protein kinase C zinc-binding repeat homology 4e-33

[SUPFAM] protein kinase C delta 2e-32

[SUPFAM] cGMP-dependent protein kinase 3e-33

[SUPFAM] protein kinase cdrl le-45

[SUPFAM] kinase-related transforming protein 2e-50

[SUPFAM] Ca2+/calmodulιn-dependent protein kinase I 8e-42

[SUPFAM] kinase interaction domain homology 7e-41

[SUPFAM] gag-akt polyprotein le-34

[PROSITE] PROTEIN_KINASE_ATP 1

[PROSITE] MYRISTYL 3

[PROSITE] AMIDATION 2

[PROSITE] CAMP_PHOSPHO_SITE 4

[PROSITE] CK2_PHOSPHO_SITE 15

[PROSITE] TYR_PHOSPHO_SITE 2

[PROSITE] PKC_PHOSPHO_SITE 10

[PROSITE] ASN_GLYCOSYLATION 2

[PROSITE] PROTEIN_KINASE_ST 1

[PFAM] Eukaryotic protein kinase domain

[KW] Irregular

[KW] 3D

[KW] LOW COMPLEXITY 12.31 %

SEQ MVMADGPRHLQRGPVRVGFYDIEGTLGKGNFAVVKLGRHRITKTEVAIKIIDKSQLDAVN SEG IctpE EEECTTTEEEEEEEETTTTEEEEEEEEEHHHHHHHC

SEQ LEKIYREVQIMKMLDHPHIIKLYQVMETKSMLYLVTEYAKNGEIFDYLANHGRLNESEAR SEG IctpE HHHHHHHHHHHHCCCTTTBCCEEEEEEETTEEEEEEECTTTTBHHHHHHHHCCCCHHHHH

SEQ RKFWQILSAVDYCHGRKIVHRDLKAENLLLDNNMNIKIADFGFGNFFKSGELLATWCGSP SEG IctpE HHHHHHHHHHHHHHHCCEECCCCCGGGEEETTTTCEEECCTTTTEETT-TTBC-CCCCCG

SEQ PYAAPEVFEGQQYEGPQLDIWSMGVVLYVLVCGALPFDGPTLPILRQRVLEGRFRIPYFM SEG IctpE GGCCHHHHHCCCBC-HHHHHHHHHHHHHHHHHCCTTTTTTTHHHHHHHHHHCCCCCTTTT

SEQ SEDCEHLIRRMLVLDPSKRLTIAQIKEHKWMLIEVPVQRPVLYPQEQENEPSIGEFNEQV SEG IctpE CHHHHHHHHHTTTTTGGGTTTHHHHHHCGG

SEQ LRLMHSLGIDQQKTIESLQNKSYNHFAAIYFLLVERLKSHRSSFPVEQRLDGRQRRPSTI

SEG IctpE

SEQ AEQTVAKAQTVGLPVTMHSPNMRLLRSALLPQASNVEAFSFPASGCQAEAAFMEEECVDT SEG IctpE

SEQ PKVNGCLLDPVPPVLVRKGCQSLPSNMMETSIDEGLETEGEAEEDPAHAFEAFQSTRSGQ SEG xxxxxxxxxxx IctpE

SEQ RRHTLSEVTNQLVVMPGAGKIFSMNDSPSLDSVDSEYDMGSVQRDLNFLEDNPSLKDIML SEG IctpE ON O

HMM *YeιgRιIGeGsFGtVYkCιWr .TGelVAIKIIkkrsms F1REI

Y I++++G+G+F++V+++++R T +VAIKII+K++++ + RE+ Query 20 YDIEGTLGKGNFAVVKLGRHRITKTEVAIKIIDKSQLDAVNLEKIYREV 68

HMM qlMRrLnHPNIIRFYDwFedddDHIYMIMEYMeGGDLFDYIrrngpMsEw

QIM++L+HP+II++Y ++E +++ +Y+++EY+ +G++FDY+ ++G+++E Query 69 QIMKMLDHPHIIKLYQVME-TKSMLYLVTEYAKNGEIFDYLANHGRLNES 117

HMM elrflMyQILrGMeYLHSMgllHRDLKPENILIDeNgqlKIcDFGLARqM

E+R+ ++QIL++++Y+H ++I+HRDLK+EN+L+D+N++IKI+DFG+ ++ Query 118 EARRKFWQILSAVDYCHGRKIVHRDLKAENLLLDNNMNIKIADFGFGNFF 167

HMM nnYerMttfCGTPWYMMAPEVIImg.nyYttkVDMWSFGCILWEMMTGep

+++E++ T CG+P+Y APEV +G +Y +++ D+WS+G++L+ +++G + Query 168 KSGELLATWCGSPPYA-APEV-FEGQQYEGPQLDIWSMGVVLYVLVCGAL 215

HMM PFyddnMemlmrliqrfrrpfWpnCSeElyDFMrwCWnyDPekRPTFrQI

PF++ ++ + + +++ R+++++ +SE++ +++R+++ +DP+KR+T+ QI Query 216 PFDGPTLPILRQRVLEGRFRIPYFMSEDCEHLIRRMLVLDPSKRLTIAQI 265

HMM LnHPWF*

+H W+ Query 266 KEHKWM 271

DKFZphtes3_17flO

group: testes derived

DKFZphtes3_15jl8 encodes a novel 710 ammo acid protein with weak similarity to neurofilament proteins .

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . similarity to neurofilament proteins

Sequenced by GBF

Locus : unknown

Insert length: 2533 bp

Poly A stretch at pos. 2507, no polyadenylation signal found

1 CTTCAGTTCA ACTAAAAATG GACAGATCTC AGCAGACCAG CCGTACAGGA 51 TACTGGACCA TGATGAACAT CCCCCCTGTA GAAAAAGTGG ACAAGGAACA

101 ACAGACATAC TTTAGTGAAT CAGAAATAGT GGTTATTTCC AGGCCAGATA

151 GTTCTTCTAC AAAGTCAAAG GAAGATGCCC TGAAACATAA ATCGTCGGGA

201 AAGATTTTTG CTAGTGAACA CCCTGAATTT CAACCAGCAA CAAACAGCAA

251 TGAAGAAATT GGGCAGAAAA ATATCAGCAG AACTTCATTT ACTCAGGAGA

301 CTAAAAAAGG TCCCCCAGTA CTTTTAGAAG ATGAGCTTAG GGAAGAAGTA

351 ACTGTACCTG TTGTACAAGA AGGTTCTGCT GTTAAAAAAG TGGCTTCTGC

401 TGAAATAGAG CCTCCATCAA CAGAAAAATT CCCAGCTAAA ATACAGCCTC

451 CATTAGTTGA AGAGGCCACT GCTAAAGCGG AGCCCAGACC TGCTGAAGAG

501 ACCCATGTCC AAGTACAGCC ATCAACTGAA GAGACTCCTG ATGCTGAGGC

551 AGCCACTGCA GTTGCGGAGA ATTCTGTTAA AGTTCAGCCT CCACCTGCTG

601 AAGAGGCCCC TTTAGTGGAG TTTCCTGCTG AAATTCAGCC TCCATCAGCT

651 GAAGAGTCTC CTTCTGTAGA GCTTCTGGCT GAAATTCTGC CTCCATCAGC

701 TGAAGAGTCC CCTTCAGAAG AGCCTCCTGC TGAAATTCTG CCTCCACCAG

751 CTGAAAAGTC TCCTTCAGTA GAGCTTCTTG GTGAAATTCG GTCTCCCTCA

801 GCACAAAAGG CTCCCATTGA AGTACAGCCT TTACCAGCTG AGGGCGCCCT

851 TGAAGAGGCC CCAGCTAAAG TAGAGCCTCC CACTGTTGAA GAGACCCTTG

901 CTGAAGTTCA GCCTCTATTA CCTGAAGAGG CTCCTAGAGA AGAGGCTCGA

951 GAACTTCAGC TTTCAACAGC TATGGAGACC CCTGCAGAAG AGGCTCCTAC 1001 TGAATTTCAG TCTCCATTAC CTAAAGAGAC CACTGCAGAA GAGGCCTCTG 1051 CTGAAATTCA GCTTCTAGCA GCTACGGAGC CTCCTGCAGA TGAAACTCCT 1101 GCCGAAGCTC GGTCTCCACT ATCTGAGGAG ACTTCTGCAG AAGAGGCTCA 1151 TGCTGAAGTT CAATCTCCAT TAGCTGAAGA GACCACTGCA GAAGAGGCCT 1201 CTGCTGAAAT TCAGCTTCTA GCAGCTATAG AGGCTCCTGC AGATGAAACT 1251 CCTGCTGAAG CTCAGTCTCC ACTATCTGAG GAGACTTCTG CAGAAGAGGC 1301 TCCTGCTGAA GTTCAGTCTC CATCAGCTAA GGGAGTTTCT ATAGAAGAGG 1351 CCCCTCTTGA GCTTCAGCCT CCATCAGGTG AAGAGACCAC TGCAGAAGAG 1401 GCCTCTGCTG CAATTCAGCT TCTAGCAGCT ACAGAGGCTT CTGCAGAAGA 1451 GGCTCCTGCT GAAGTTCAGC CTCCACCAGC TGAGGAGGCC CCCGCTGAAG 1501 TTCAGCCTCC ACCAGCTGAG GAGGCCCCCG CTGAAGTTCA GCCTCCACCA 1551 GCTGAGGAGG CCCCCGCTGA AGTTCAGCCT CCACCAGCTG AGGAGGCCCC 1601 CGCTGAAGTT CAGCCTCCAC CAGCTGAGGA GGCCCCCGCT GAAGTTCAGC 1651 CTCCACCAGC TGAGGAGGCC CCCTCTGAAG TTCAGCCTCC ACCAGCTGAG 1701 GAGGCCCCTG CTGAAGTTCA GTCTCTACCA GCTGAGGAGA CTCCTATAGA 1751 AGAGACCCTT GCTGCAGTAC ACTCTCCCCC AGCTGATGAT GTCCCTGCAG 1801 AAGAGGCCTC CGTTGACAAA CATTCCCCAC CAGCTGATTT GCTTCTGACT 1851 GAGGAGTTTC CTATAGGAGA GGCCTCTGCT GAAGTTTCAC CTCCACCATC 1901 TGAACAAACC CCTGAAGATG AGGCTCTGGT AGAGAATGTG TCTACAGAAT 1951 TTCAGTCACC GCAGGTGGCA GGAATTCCAG CAGTAAAATT AGGATCGGTT 2001 GTTTTGGAAG GTGAAGCAAA ATTTGAAGAG GTTTCAAAAA TCAATTCTGT 2051 CCTTAAAGAT TTGTCTAATA CCAATGATGG ACAGGCTCCC ACTCTTGAAA 2101 TAGAAAGTGT TTTTCATATA GAATTAAAAC AACGTCCTCC TGAACTGTAG 2151 TCAGGTTGTA CCTAAGCTAG CAATCAGAAG CTACATGGTT TTGGAAGAAC 2201 ATACTTTAGA AAAGGGTGGG CAGCAGGAAG TAGCTTTGTC AATAAGGCAA 2251 ATTAAAGGGG ACCCCAAGAC TTGGAATACA GGTTGGAAAA TGAACAATAA 2301 AAACTGTAGC AGCATAAAAT TACTTGTGTT AATTTCATTC AAATTTATGG 2351 CATGAAAAAT ACCTATTTTG AAAGTAAGTT TATAATTGAA AAAAATTGCT 2401 TAAAATATCC TTCCTACAGT AAACTTGTTG ACACGAGTAA AGTTTAATCT 2451 GCAGCCATCT TTTCTTGTCT TTGCCTTCCC TTTATAAGTA AATATAGTTT 2501 CTAGTGGAAA AAAAAAAAAA AAAAAAAAAA AAA

BLAST Results No BLAST result

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 18 bp to 2147 bp; peptide length: 710 Category: similarity to known protein Classification: unclassified

1 MDRSQQTSRT GYWTMMNIPP VEKVDKEQQT YFSESEIVVI SRPDSSSTKS

51 KEDALKHKSS GKIFASEHPE FQPATNSNEE IGQKNISRTS FTQETKKGPP

101 VLLEDELREE VTVPVVQEGS AVKKVASAEI EPPSTEKFPA KIQPPLVEEA

151 TAKAEPRPAE ETHVQVQPST EETPDAEAAT AVAENSVKVQ PPPAEEAPLV

201 EFPAEIQPPS AEESPSVELL AEILPPSAEE SPSEEPPAEI LPPPAEKSPS

251 VELLGEIRSP SAQKAPIEVQ PLPAEGALEE APAKVEPPTV EETLAEVQPL

301 LPEEAPREEA RELQLSTAME TPAEEAPTEF QΞPLPKETTA EEASAEIQLL

351 AATEPPADET PAEARSPLSE ETSAEEAHAE VQSPLAEETT AEEASAEIQL

401 LAAIEAPADE TPAEAQSPLS EETSAEEAPA EVQSPSAKGV SIEEAPLELQ

451 PPSGEETTAE EASAAIQLLA ATEASAEEAP AEVQPPPAEE APAEVQPPPA

501 EEAPAEVQPP PAEEAPAEVQ PPPAEEAPAE VQPPPAEEAP AEVQPPPAEE

551 APSEVQPPPA EEAPAEVQSL PAEETPIEET LAAVHSPPAD DVPAEEASVD

601 KHSPPADLLL TEEFPIGEAS AEVSPPPSEQ TPEDEALVEN VSTEFQSPQV

651 AGIPAVKLGΞ VVLEGEAKFE EVSKINSVLK DLSNTNDGQA PTLEIESVFH 701 IELKQRPPEL

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_17f10, frame 3

PIR:A37221 neurofilament triplet H protein - rat, N = 1, Score = 480, P = 7.4e-43

TREMBL :RNNFLH_1 Rat heavy neurofilament subunit (NF-H) mRNA, 3' end., N = 1, Score = 475, P = le-42

>PIR:A37221 neurofilament triplet H protein - rat Length = 1,072

HSPs:

Score = 480 (72.0 bits), Expect = 7.4e-43, P = 7.4e-43 Identities = 185/622 (29%), Positives = 320/622 (51%)

Query: 33 SESEIVVISRPDSSSTKSKEDALKHKSSGKIFASEHPEFQPATNSNEEIGQKNISRTSFT 92

SE +1 V+ + + + +E + + + ++ E E Q E G + + TS Sbjct: 436 SEEKIKVVEKSEKETVIVEEQTEEIQVTEEVTEEEDKEAQGEEEEEAEEGGEEAATTSPP 495

Query: 93 QETKKGPPVLLEDELREEVTVPVVQEGSAVKKVASAEIEPPSTEKFPAKIQPPLVEEATA 152

E P + ++EE P + A K + AE + P+ K PA+++ P ++ A Sbjct: 496 AEEAASPEKETKSPVKEEAKSPAEAKSPAEAK-SPAEAKSPAEVKSPAEVKSPAEAKSPA 554

Query: 153 KAEPRPAEETHVQVQPSTEETPDAEAATAVAENSVKVQPPPAEEAP-LVEFPAEIQPPSA 211

+A+ PAE V+ P+T ++P + A A++ +V+ P ++P + PAE + P+ Sbjct: 555 EAKS-PAE VK-SPATVKSPAEAKSPAEAKSPAEVKSPATVKSPGEAKSPAEAKSPAE 609

Query: 212 EESP-SVELLAEILPPSAEESPSE-EPPAEILPPPAEKSPS-VELLGEIRSPSAQKAPIE 268

+SP + AE P++ +SP E + PAE P KSP+ V+ E +SP+ K+P+ Sbjct: 610 VKSPVEAKSPAEAKSPAΞVKSPGEAKSPAEAKSPAEVKSPATVKSPVEAKSPAEVKSPVT 669

Query: 269 VQPLPAEGALEEAPAKVEPPTVEETLAEVQPLLPEEAPREEARELQLSTAMETPAE-EAP 327

V+ PAE ++P +V+ P ++ +E + ++P E A+ ++PAE ++P Sbjct: 670 VKS-PAEA KSPVEVKSPASVKSPSEAKSPAGAKSPAE-AKS PVVAKSPAEAKSP 721

Query: 328 TEFQSPLPKETTAEEASAEIQLLAATEPPAD-ETPAEARSPLSEETSAEEAHAEVQS 383

E + P ++ AE S A + PA+ ++PAEA+SP+ E S E+A + V+ Sbjct: 722 AEAKPPAEAKSPAEAKSP AEAKSPAEAKSPAEAKSPV-EVKSPEKAKSPVKEGAK 775

Query: 384 PLAEETTAEEASAEIQLLAAIEAPAD-ETPAEAQSPLSEET-SAEEAPA-EVQSPSAKGV 440 LAE + E+A + ++ 1+ PA+ ++P +A+SP+ EE S E+A +V+SP AK Sbjct: 776 SLAEAKSPEKAKSPVK—EEIKPPAEVKSPEKAKSPMKEEAKSPEKAKTLDVKSPEAKTP 833

Query: 441 SIEEA—PLELQPPSGEETTA-EEASAAIQLLAATEAΞA EEAPAEVQPPPAEEAPAE 494

+ EEA P +++ P ++ A EEA + + TE A EE + V+ A+E P + Sbjct: 834 AKEEAKRPADIRSPEQVKΞPAKEEAKSPEKEETRTEKVAPKKEEVKSPVEEVKAKEPPKK 893

Query: 495 VQPPPAEEAP-AEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAPS 553

V+ P EV+ +EAP E Q P AEE + P +++P E + EEA Sbjct: 894 VEEEKTPATPKTEVKESKKDEAPKEAQKPKAEEKEPLTEKP—KDSPGEAKK EEAKE 948

Query: 554 EVQPPPAEEAPAEV QSLP AEETPIEETL—AAVHSPPADDVPAEEASVD-KHS 603

+ P EE PA++ ++ P AE+ +E + P ++VPA D K Sbjct: 949 KKAAAPEEETPAKLGVKEEAKPKEKAEDAKAKEPSKPSEKEKPKKEEVPAAPEKKDTKEE 1008

Query: 604 PPADLLLTEEFPIGEASAEVSPP—PSEQT-PEDEALVENVSTEFQSPQ 649

+ EE P +A A+ P E + P+ E ++ ST+ + Q Sbjct: 1009 KTTESKKPEEKPKMQAKAKEEDKGLPQEPSKPKTEKAEKSSSTDQKDSQ 1057

Score = 473 (71.0 bits), Expect = 4.8e-42, P = 4.8e-42 Identities = 184/628 (29%), Positives = 310/628 (49%)

Query: 18 IPPVEKVDKEQQTYFSESEIVVISRP DSSSTKSKEDALKHKSSGKIFASEHPEFQPA 74

I VEK +KE ++E + ++ + E+ + + G+ A+ P + A Sbjct: 440 IKVVEKSEKETVIVEEQTEEIQVTEEVTEEEDKEAQGEEEEEAEEGGEEAATTSPPAEEA 499

Query: 75 TNSNEEIGQKNISRTSFTQETKKGPPVLLEDELREEVTVPVVQEGSAVKKVASAEIEPPS 134

+ +E + + + + K P E + E P + A K + AE + P+ Sbjct: 500 ASPEKET-KSPVKEEAKSPAEAKSPA EAKSPAEAKSPAEVKSPAEVK-SPAEAKSPA 554

Query: 135 TEKFPAKIQPPLVEEATAKAEPRPAEETHVQVQ-PΞTEETPDAEAATAVAENSVKVQPPP 193

K PA+++ P ++ A+A+ ++ +V+ P+T ++P + A A++ +V+ P Sbjct: 555 EAKSPAEVKSPATVKSPAEAKSPAEAKSPAEVKSPATVKSPGEAKSPAEAKSPAEVKSPV 614

Query: 194 AEEAPL-VEFPAEIQPPSAEESPS-VELLAEILPPSAEESPSE-EPPAEILPPPAEKSPS 250

++P + PA ++ P +SP+ + AE+ P+ +SP E + PAE+ P KSP+ Sbjct: 615 EAKSPAEAKSPASVKSPGEAKSPAEAKSPAEVKSPATVKSPVEAKSPAEVKSPVTVKSPA 674

Query: 251 -VELLGEIRSPSAQKAPIEVQ-PLPAEGALE-EAPAKVEPPTVEETLAEVQPLLPEEAPR 307

+ E++SP++ K+P E + P A+ E ++P + P ++ AE +P ++P Sbjct: 675 EAKSPVEVKSPASVKSPSEAKSPAGAKΞPAEAKSPVVAKSPAEAKSPAEAKPPAEAKSPA 734

Query: 308 EEARELQLSTAME--TPAE-EAPTEFQSP LP-KE TTAEEASAEIQLLAATE-- 354

E + + E +PAE ++P E +SP P KE + AE S E E Sbjct: 735 EAKSPAEAKSPAEAKSPAEAKSPVEVKSPEKAKSPVKEGAKSLAEAKSPEKAKSPVKEEI 794

Query: 355 -PPAD-ETPAEARSPLSEET-SAEEAHA-EVQSPLAEETTAEEAS—AEIQLLAAIEAPA 408

PPA+ ++P +A+SP+ EE S E+A +V+SP A+ EEA A+I+ +++PA Sbjct: 795 KPPAEVKSPEKAKSPMKEEAKSPEKAKTLDVKSPEAKTPAKEEAKRPADIRSPEQVKSPA 854

Query: 409 DETPAEAQSPLΞEETSAEE-APA--EVQSPSAKGVSIEEAPLELQPPSGEETTAEEASAA 465

E EA+SP EET E+ AP EV+SP +EE + +PP E EE + A Sbjct: 855 KE EAKSPEKEETRTEKVAPKKEEVKSP VEEVKAK-EPPKKVE EEKTPA 901

Query: 466 IQLLAATEASAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAE 525

E+ +EAP E Q P AEE + P +++P E + A+E A P E Sbjct: 902 TPKTEVKESKKDEAPKEAQKPKAEEKEPLTEKP--KDSPGEAKKEEAKEKKAAA PEE 956

Query: 526 EAPAEV QPPPAEEAPAEVQPPPAEEAPSEVQPPPAEEAPAEVQSLPAEETPIEETL 581

E PA++ + P E+A P++ PSE + P EE PA + +E E+ Sbjct: 957 ETPAKLGVKEEAKPKEKAEDAKAKEPSK—PSEKEKPKKEEVPAAPEKKDTKEEKTTESK 1014

Query: 582 AAVHSPPADDVPAEEASVDKHSPPADLL-LTEEFPIGEASAEVSPPPSEQTPEDEA 636

P EE DK P TE+ ++ + PSE+ PED+A Sbjct: 1015 KPEEKPKMQAKAKEE DKGLPQEPSKPKTEKAEKSSSTDQKDSQPSEKAPEDKA 1067

Score = 421 (63.2 bits), Expect = 3.7e-36, P = 3.7e-36 Identities = 162/540 (30%), Positives = 275/540 (50%)

Query: 135 TEKFPAKIQPPLVEEATAKAEPR PAEETHVQVQPSTEETPDAEAATAVAENSVKV 189

TE P KI P + K+E + +E+ V V+ TEE E T E + Sbjct: 419 TEGLP-KI-PSMSTHIKVKSEEKIKVVEKSEKETVIVEEQTEEIQVTEEVTE—EEDKEA 474

Query: 190 QPPPAEEAPLVEFPAEIQPPSAEESPSVELLAEILPPSAEE—SPSE-EPPAEILPPPAE 246

Q EEA A P AEE+ S E E P EE SP+E + PAE P Sbjct: 475 QGEEEEEAEEGGEEAATTSPPAEEAASPE—KETKΞPVKEEAKSPAEAKSPAEAKSPAEA 532

Query: 247 KSPSVELLGEIRSPSAQKAPIEVQPLPAEGALEEAPAKVEPPTVEETLAEVQPLLPEEAP 306

KSP+ E++SP+ K+P E + PAE ++PA+V+ P ++ AE + ++P Sbjct: 533 KSPA EVKSPAEVKSPAEAKS-PAEA KSPAEVKSPATVKSPAEAKSPAEAKSP 583 Query: 307 REEARELQLSTAME—TPAE-EAPTEFQSPLPKETTAEEAS-AEIQLLAATEPPAD-ETP 361

E + + E +PAE ++P E +SP+ ++ AE S A ++ + PA+ ++P Sbjct: 584 AEVKSPATVKSPGEAKSPAEAKSPAEVKSPVEAKSPAEAKSPASVKSPGEAKΞPAEAKSP 643

Query: 362 AEARSPLSEETSAE-EAHAEVQΞPLAEETTAEEASAEIQLLAAIEAPAD-ETPAEAQSPL 419

AE +SP + ++ E ++ AEV+SP+ ++ AE A + ++ +++PA ++P+EA+SP Sbjct: 644 AEVKSPATVKSPVEAKSPAEVKSPVTVKSPAE-AKSPVE VKSPASVKSPSEAKSP- 697

Query: 420 SEETSAEEAPAEVQSPS-AKGVΞIEEAPLELQPPSGEETTAEEASAAIQLLAATEASAEE 478

+ ++PAE +SP AK + ++P E +PP+ ++ AE S A A + A A+ Sbjct: 698 AGAKSPAEAKSPVVAKSPAEAKSPAEAKPPAEAKSPAEAKSPAE AKSPAEAK- 749

Query: 479 APAEVQPPPAEEAPAEVQPPPAEEAP—AEVQPPPAEEAPA--EVQPPPAEEAPAEVQPP 534

+PAE + P ++P + + P E A AE + P ++P E++PP ++P + + P Sbjct: 750 SPAEAKSPVEVKSPEKAKSPVKEGAKSLAEAKSPEKAKSPVKEEIKPPAEVKSPEKAKSP 809

Query: 535 PAEEAPAEVQPPPAEEAPSEVQPPPAEEA—PAEVQSLPAEETPIEETLAAVHSPPADDV 592

EEA + + + E + P EEA PA+++S ++P +E SP ++ Sbjct: 810 MKEEAKSPEKAKTLDVKSPEAKTPAKEEAKRPADIRSPEQVKSPAKEE AKSPEKEET 866

Query: 593 PAEEASVDKHS--PPADLLLTEEFPIGEASAEVSPPPSEQTPEDEALVENVSTEFQSPQV 650

E+ + K P + + +E P + E P + +T E+ + E Q P+ Sbjct: 867 RTEKVAPKKEEVKSPVEEVKAKEPP—KKVEEEKTPATPKTEVKESKKDEAPKEAQKPKA 924

Query: 651 AGIPAVKLGSVVLEGEAKFEEVSK 674

+ GEAK EE + Sbjct: 925 EEKEPLTEKPKDSPGEAKKEEAKE 948

Score = 406 (60.9 bits), Expect = 1.7e-34, P = 1.7e-34 Identities = 123/390 (31%), Positives = 213/390 (54%)

Query: 308 EEARELQLSTAMETPAEEAPTEFQSPLPKETTAEEASAEIQLLAATEPPADETPA EA 364

E+ E+Q++ E EE E Q +E AEE E A T PPA+E + E Sbjct: 455 EQTEEIQVT EEVTEEEDKEAQGE—EEEEAEEGGEEA ATTSPPAEEAASPEKET 506

Query: 365 RSPLSEETSAEEAHAEVQSPLAEETTAEEAS-AEIQLLAAIEAPAD-ETPAEAQSPLSEE 422

+SP+ EE + AE +SP ++ AE S AE++ A +++PA+ ++PAEA+SP + Sbjct: 507 KSPVKEEAKSP AEAKSPAEAKSPAEAKSPAEVKSPAEVKSPAEAKSPAEAKSPAEVK 563

Query: 423 TSAE-EAPAEVQSPS-AKGVSIEEAPLELQPPSGEETTAEEASAAIQLLAATEASAEEAP 480

+ A ++PAE +SP+ AK + ++P ++ P GE + EA + ++ + EA ++P Sbjct: 564 SPATVKSPAEAKSPAEAKSPAEVKSPATVKSP-GEAKSPAEAKSPAEVKSPVEA KSP 619

Query: 481 AEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAP 540

AE + P + ++P E + P ++PAEV+ P ++P E + P ++P V+ P ++P Sbjct: 620 AEAKSPASVKSPGEAKSPAEAKSPAEVKSPATVKSPVEAKSPAEVKΞPVTVKSPAEAKSP 679

Query: 541 AEVQPPPAEEAPSEVQPPPAEEAPAEVQSLPAEETPIEETLAAVHSPPAD-DVPAEEASV 599

EV+ P + ++PSE + P ++PAE +S ++P E A PPA+ PAE S Sbjct: 680 VEVKSPASVKSPSEAKSPAGAKSPAEAKSPVVAKSPAEAKSPAEAKPPAEAKSPAEAKSP 739

Query: 600 DKHSPPADLLLTEEFPIGEASAEVSPPPSEQTPEDEALVENVSTEFQSPQVAGIPAVKLG 659

+ PA+ E ++ EV P ++P E ++++ E +SP+ A P VK Sbjct: 740 AEAKSPAEAKSPAE AKSPVEVKSPEKAKSPVKEG-AKSLA-EAKSPEKAKSP-VK-E 792

Query: 660 SVVLEGEAKFEEVSKINSVLKDLSNTNDGQAPTLEIES 697

+ E K E +K S +K+ + + + +A TL+++S Sbjct: 793 EIKPPAEVKSPEKAK—ΞPMKEEAKSPE-KAKTLDVKS 827

Score = 255 (38.3 bits), Expect = 5.5e-18, P = 5.5e-18 Identities = 124/420 (29%), Positives = 199/420 (47%)

Query: 252 ELLGEIRSPSAQKAPIEVQPLPA EGALEEAPAKVEPPTVEETLAEVQPLLPEEAP 306

ELLG+I+ A +A + + A AL E A++E TV+ TL + Sbjct: 236 ELLGQIQGCGAAQAQAQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDR 295

Query: 307 REEARELQLSTAMETPAEEAPTEFQSPLPKETTAEEASAEIQLLAATEPPADETPAEARS 366

EA ++ + AM + EE TE++ L TT E++ L +T+ + +E Sbjct: 296 LSEAAKVN-TDAMRSAQEEI-TEYRRQLQARTT ELEALKSTKESLERQRSELED 347

Query: 367 PLSEE-TSAEEAHAEVQSPLAEETTAEEASA—EIQLLAAIEAPAD-ETPAEAQSPLSEE 422

+ S ++A ++ + L T E A+ E Q L ++ D E A + EE Sbjct: 348 RHQVDMASYQDAIQQLDNEL-RNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEE 406

Query: 423 TSAEEAPAEV QSPS-AKGVSIE-EAPLELQPPSGEETT-AEEASAAIQLLA-A 471

P+ + PS + + ++ E +++ Ξ +ET EE + IQ+

Sb_jct: 407 CRIGFGPSPFSLTEGLPKIPSMSTHIKVKΞEEKIKVVEKSEKETVIVEEQTEEIQVTEEV 466

Query: 472 TEASAEEAPAEVQPPPAEEAPAEVQP—PPAEEAPA EVQPPPAEEA—PAEVQPPPA 524

TE +EA E + AEE E PPAEEA + E + P EEA PAE + P Sbjct: 467 TEEEDKEAQGE-EEEEAEEGGEEAATTSPPAEEAASPEKETKSPVKEEAKSPAEAKSPAE 525 Query: 525 EEAPAEVQPPPAEEAPAEVQPPPAEEAPSEVQPPPAEEAPAEVQSLPAE-ETPIE-ETLA 582

++PAE + P ++PAEV+ P ++P+E + P ++PA V+S PAE ++P E ++ A Sbjct: 526 AKSPAEAKSPAEVKSPAEVKSPAEAKSPAEAKSPAEVKSPATVKS-PAEAKSPAEAKSPA 584

Query: 583 AVHSPPADDVPAEEASVDKHSPPADLLLTEEFPIGEASAEVSPPPSEQTP-EDEALVENV 641

V SP P E S + PA++ E ++ AE P S ++P E ++ E Sbjct: 585 EVKSPATVKSPGEAKSPAEAKSPAEVKSPVE AKSPAEAKSPASVKSPGEAKSPAEAK 641

Query: 642 S-TEFQSPQVAGIP 654

S E +SP P Sbjct: 642 SPAEVKSPA VKSP 655

Score = 253 (38.0 bits), Expect = 9.0e-18, P = 9.0e-18 Identities = 115/364 (31%), Positives = 166/364 (45%)

Query: 110 EVTVPVVQEGSAVKKVASAEIEPPSTEKFPAKIQPPLVEEATAKAEPRPAE-ETHVQVQ- 167

E PVV + A K + AE +PP+ K PA+ + P ++ A+A+ PAE ++ V+V+ Sbjct: 705 EAKSPVVAKSPAEAK-SPAEAKPPAEAKSPAEAKSPAEAKSPAEAKS-PAEAKΞPVEVKS 762

Query: 168 PSTEETPDAEAATAVAE—NSVKVQPPPAEEA—PL-VEFPAEIQPPSAEE—SPSVELL 220

P ++P E A ++AE + K + P EE P V+ P + + P EE SP Sbjct: 763 PEKAKSPVKEGAKSLAEAKSPEKAKSPVKEEIKPPAEVKSPEKAKSPMKEEAKSPEKAKT 822

Query: 221 AEILPPSAEESPSEEP—PAEILPPPAEKSPSVELLGEIRSPSAQKAPIE-VQPLPAE— 275

++ P A+ EE PA+I P KSP+ E E +SP ++ E V P E Sbjct: 823 LDVKSPEAKTPAKEEAKRPADIRSPEQVKSPAKE EAKSPEKEETRTEKVAPKKEEVK 879

Query: 276 GALEEAPAKVEPPTVEETLAEVQPLLPEEAPREEARELQLSTAMETPAEEA-P-TEFQSP 333

+EE AK P VEE E P P+ +E ++ A + AEE P TE Sbjct: 880 SPVEEVKAKEPPKKVEE EKTPATPKTEVKESKKDEAPKEAQKPKAEEKEPLTEKPKD 936

Query: 334 LPKETTAEEASAEIQLLAATEPPADETPAE—ARSPLSEETSAEEAHA-EVQSPLAEETT 390

P E EEA + AA P +ETPA+ + + AE+A A E P +E Sbjct: 937 SPGEAKKEEAKEK KAAA—PEEETPAKLGVKEEAKPKEKAEDAKAKEPSKPSEKEKP 991

Query: 391 A-EEASAEIQLLAAIEAPADETPAEAQSPLSEETSAEEAPAEVQSPSA-KGVSIEEAPLE 448

EE A + E E+ + P + + EE Q PS K E++ Sbjct: 992 KKEEVPAAPEKKDTKEEKTTESKKPEEKPKMQAKAKEEDKGLPQEPSKPKTEKAEKSSST 1051

Query: 449 LQPPSGEETTAEEASAA 465

Q S A E AA Sbjct: 1052 DQKDSQPSEKAPEDKAA 1068

Pedant information for DKFZphtes3_17f10, frame 3

Report for DKFZphtes3_17f10.3

[LENGTH] 710

[MW] 75131.94

[pi] 4.02

[KW] All_Alpha

[KW] LOW_COMPLEXITY 34.08 %

SEQ MDRSQQTSRTGYWTMMNIPPVEKVDKEQQTYFSESEIVVISRPDSSSTKSKEDALKHKSS SEG

PRD cccccccccccccccccccceeehhhhhhhccccceeeeeccccccccchhhhhhhhccc

SEQ GKIFASEHPEFQPATNSNEEIGQKNISRTSFTQETKKGPPVLLEDELREEVTVPVVQEGS SEG

PRD cceeecccccccccccccccccccccccccceeeecccccchhhhhhhhhheeeeccccc

SEQ AVKKVASAEIEPPSTEKFPAKIQPPLVEEATAKAEPRPAEETHVQVQPSTEETPDAEAAT

SEG xxxxxxxxxxx

PRD chhhhhhhccccccccccccccccchhhhhhhhhccccccceeeecccccccccchhhhh

SEQ AVAENSVKVQPPPAEEAPLVEFPAEIQPPSAEESPSVELLAEILPPSAEESPSEEPPAEI

SEG xxxx xxxxxxxxxxxxxxxxxxxx

PRD hhhhhcccccccccccceeeeccccccccccccccchhhhhhcccccccccccccccccc

SEQ LPPPAEKΞPSVELLGEIRSPSAQKAPIEVQPLPAEGALEEAPAKVEPPTVEETLAEVQPL

SEG xxxxxx xxxxxxxxxxxxx xxx

PRD cccccccccccccccccccccccccccccccccchhhhhcccccccccchhhhhhhhhhc

SEQ LPEEAPREEARELQLSTAMETPAEEAPTEFQSPLPKETTAEEASAEIQLLAATEPPADET

SEG xxxxxxxxxxxxxxx....xxxxxxxxxx xxxxxxxxxx

PRD ccccchhhhhhhhhhhhhhccccccccccccccccchhhhhhhhhhhhhhhhcccccccc SEQ PAEARSPLSEETSAEEAHAEVQSPLAEETTAEEASAEIQLLAAIEAPADETPAEAQSPLS

SEG xxxx....xxxxxxxxxxxx xxxxxxxxxxxx xxxx

PRD cccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccc

SEQ EETSAEEAPAEVQSPSAKGVSIEEAPLELQPPSGEETTAEEASAAIQLLAATEASAEEAP

SEG xxxxxxxxxxx xxxxxxxxxxx xxxxxxxx

PRD chhhhhcccccccccccceeecccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhc

SEQ AEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAP

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ AEVQPPPAEEAPSEVQPPPAEEAPAEVQSLPAEETPIEETLAAVHSPPADDVPAEEASVD

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD ccccccccccccccccccccccccccccccccccchhhhhhhhhcccccccccccccccc

SEQ KHSPPADLLLTEEFPIGEASAEVSPPPΞEQTPEDEALVENVSTEFQSPQVAGIPAVKLGS

SEG

PRD cccccceeeeeccccccccccccccccccccccchhhhhccccccccccccccccccccc

SEQ VVLEGEAKFEEVSKINSVLKDLSNTNDGQAPTLEIESVFHIELKQRPPEL

SEG

PRD eeeehhhhhhhhccceeeeeeccccccccceeeehhhhhhhhhhcccccc

(No Prosite data available for DKFZphtes3_17f10.3) (No Pfam data available for DKFZphtes3_17f10.3)

DKFZphtes3_17117

group: metabolism

DKFZphtes3_17117 encodes a novel 626 ammo acid protein with similarity to transketaloases (EC 2.2.1.1) .

The novel protein contains a ATP/GTP-binding site motif A (P-loop) . It is a new testis- specific transketolase. Transketolase requires thiamin pyrophosphate as cofactor and shows a wide specificity for both reactants, e.g. converts hydroxypyruvate and R-CHO into CO(2) and R- CHOH-CO-CH(2)OH.

The new protein can find application in modulation of metabolic pathways involving this transketolase activity and as a new enzyme for biotechnologic production processes. strong similarity to transketolases few EST hits (all from testis or pooled librarys containing testis) testis specific transketolase?

Sequenced by GBF

Locus : unknown

Insert length: 2688 bp

Poly A stretch at pos. 2649, polyadenylation signal at pos. 2630

1 GACAAAAGAG AGATGATGGC CAACGACGCC AAGCCCGACG TGAAGACCGT 51 GCAGGTGCTG CGGGACACAG CCAACCGCCT GCGGATCCAT TCCATCAGGG

101 CCACGTGTGC CTCTGGTTCT GGCCAGCTCA CGTCGTGCTG CAGTGCAGCG

151 GAGGTCGTGT CTGTCCTCTT CTTCCACACG ATGAAGTATA AACAGACAGA

201 CCCAGAACAC CCGGACAACG ACCGGTTCAT CCTCTCCAGG GGACATGCTG

251 CTCCTATCCT CTATGCTGCT TGGGTGGAGG TGGGTGACAT CAGTGAATCT

301 GACTTGCTGA ACCTGAGGAA ACTTCACAGC GACTTGGAGA GACACCCTAC

351 CCCGCGATTG CCGTTTGTTG ACGTGGCAAC AGGGTCCCTA GGTCAGGGAT

401 TAGGTACTGC ATGTGGAATG GCTTATACTG GCAAGTACCT TGACAAGGCC

451 AGCTACCGGG TGTTCTGCCT TATGGGAGAT GGCGAATCCT CAGAAGGCTC

501 TGTGTGGGAG GCTTTTGCTT TTGCCTCCCA CTACAACTTG GACAATCTCG

551 TGGCGGTCTT CGACGTGAAC CGCTTGGGAC AAAGTGGCCC TGCACCCCTT

601 GAGCATGGCG CAGACATCTA CCAGAATTGC TGTGAAGCCT TTGGATGGAA

651 TACTTACTTA GTGGATGGCC ATGATGTGGA GGCCTTGTGC CAAGCATTTT

701 GGCAAGCAAG TCAAGTGAAG AACAAGCCTA CTGCTATAGT TGCCAAGACC

751 TTCAAAGGTC GGGGTATTCC AAATATTGAG GATGCAGAAA ATTGGCATGG

801 AAAGCCAGTG CCAAAAGAAA GAGCAGATGC AATTGTCAAA TTAATTGAGA

851 GTCAGATACA GACCAATGAG AATCTCATAC CAAAATCGCC TGTGGAAGAC

901 TCACCTCAAA TAAGCATCAC AGATATAAAA ATGACCTCCC CACCTGCTTA

951 CAAAGTTGGT GACAAGATAG CTACTCAGAA AACATATGGT TTGGCTCTGG 1001 CTAAACTGGG CCGTGCAAAT GAAAGAGTTA TTGTTCTGAG TGGTGACACG 1051 ATGAACTCCA CCTTTTCTGA GATATTCAGG AAAGAACACC CTGAGCGTTT 1101 CATAGAGTGT ATTATTGCTG AACAAAACAT GGTAAGTGTG GCACTAGGCT 1151 GTGCTACACG TGGTCGAACC ATTGCTTTTG CTGGTGCTTT TGCTGCCTTT 1201 TTTACTAGAG CATTCGATCA GCTCCGAATG GGAGCCATTT CTCAAGCCAA 1251 TATCAACCTT ATTGGTTCCC ACTGTGGGGT ATCCACTGGA GAAGATGGAG 1301 TCTCCCAGAT GGCCCTGGAG GATCTAGCCA TGTTCCGAAG CATTCCCAAT 1351 TGTACTGTTT TCTATCCAAG TGATGCCATC TCGACAGAGC ATGCTATTTA 1401 TCTAGCCGCC AATACCAAGG GAATGTGCTT CATTCGAACC AGCCAACCAG 1451 AAACTGCAGT TATTTATACC CCACAAGAAA ATTTTGAGAT TGGCCAGGCC 1501 AAGGTGGTCC GCCACGGTGT CAATGATAAA GTCACAGTAA TTGGAGCTGG 1551 AGTTACTCTC CATGAAGCCT TAGAAGCTGC TGACCATCTT TCTCAACAAG 1601 GTATTTCTGT CCGTGTCATC GACCCATTTA CCATTAAACC CCTGGATGCC 1651 GCCACCATCA TCTCCAGTGC AAAAGCCACA GGCGGCCGAG TTATCACAGT 1701 GGAGGATCAC TACAGGGAAG GTGGCATTGG AGAAGCTGTT TGTGCAGCTG 1751 TCTCCAGGGA GCCTGATATC CTTGTTCATC AACTGGCAGT GTCAGGAGTG 1801 CCTCAACGTG GGAAAACTAG TGAATTGCTG GATATGTTTG GAATCAGTAC 1851 CAGACACATT ATAGCAGCCG TAACACTTAC TTTAATGAAG TAAACTAGGC 1901 TTATTTCTAA AAAGTCAAGT CTATTGGCTT TGGCCCAAAA GCACTGGTAT 1951 CTTTGTATTA AATTCATGTT TATTGTCACA AAACCATTAT TTATACCTAT 2001 ACAGTTGTAC TGTTTCTTTT AAAGCAAAGC CATTTAACAT CTTTCTTCAT 2051 TCCTAATTTG GAAATTAAAG TTTACCTTTC TGTTAATCTA TGTATAAATG 2101 TTACTCTGAG TTATTAATGT GGATTTTAAA ATTGTAAGCA ATAGAATAGG 2151 AAATAAAACA ACTACCTAAT ACAAATATTT CTGATAAGAC TACAAATATC 2201 TGACTGAGCT GGGGATTAAA GTAGAGGTAA CTGTATCTTA AATGAGTATG 2251 ATTTCCTTGT AAGTTAAAAA AATTGAAATT TAATTGTAGA CTTCAATAGT 2301 CCAAGTTTTG AAGGATGTTT GAGCTTTTGT ATAATGCCAT TTATACCTGC 2351 AGTTTTACAG ATAATGTTTG ACTGCAGTTG CCTTGGAAAT TCCTCCAAAG 2401 TTTGCCTTCA TCTCTCCTCT ACAGTTTGGA GGTGATGGTG CAGCAGTGGA 2451 ACATCTCTTG ATGCACCACA CTACTTGTGT TCTGTGAAGT GATGAAAGTA 2501 TAACTGGTTC TAGTTTGCAC ACTACACACA TAGTTTTGTG AAGCTTCAGA

2551 AATGTTTTTT CTTTTCCTTG TGGCCAAACC AGTTTGTTAA TCTGATTATA

2601 TTCATCTGCT AATGATACTA AAGTTAATGT AATAAAGCAT TTAAAAATCA

2651 GAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAA

BLAST Results

No BLAST result

Medlme entries

96214928:

Amplification of the transketolase gene in desensitization-resistant mutant

YI mouse adrenocortical tumor cells.

99123875:

Properties and functions of the thiamm diphosphate dependent enzyme transketolase .

Peptide information for frame 1

ORF from 13 bp to 1890 bp; peptide length: 626 Category: strong similarity to known protein Classification: Metabolism Prosite motifs: ATP GTP A (595-603)

1 MMANDAKPDV KTVQVLRDTA NRLRIHSIRA TCASGSGQLT ΞCCSAAEVVS

51 VLFFHTMKYK QTDPEHPDND RFILSRGHAA PILYAAWVEV GDISESDLLN

101 LRKLHSDLER HPTPRLPFVD VATGSLGQGL GTACGMAYTG KYLDKASYRV

151 FCLMGDGESS EGSVWEAFAF ASHYNLDNLV AVFDVNRLGQ SGPAPLEHGA

201 DIYQNCCEAF GWNTYLVDGH DVEALCQAFW QASQVKNKPT AIVAKTFKGR

251 GIPNIEDAEN WHGKPVPKER ADAIVKLIEΞ QIQTNENLIP KSPVEDSPQI

301 SITDIKMTSP PAYKVGDKIA TQKTYGLALA KLGRANERVI VLSGDTMNST

351 FSEIFRKEHP ERFIECIIAE QNMVSVALGC ATRGRTIAFA GAFAAFFTRA

401 FDQLRMGAIS QANINLIGSH CGVSTGEDGV SQMALEDLAM FRSIPNCTVF

451 YPSDAISTEH AIYLAANTKG MCFIRTSQPE TAVIYTPQEN FEIGQAKVVR

501 HGVNDKVTVI GAGVTLHEAL EAADHLSQQG ISVRVIDPFT IKPLDAATII

551 SSAKATGGRV ITVEDHYREG GIGEAVCAAV SREPDILVHQ LAVSGVPQRG

601 KTSELLDMFG ISTRHIIAAV TLTLMK

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_17117, frame 1

SWISSPROT :TKT_MOUΞE TRANSKETOLASE (EC 2.2.1.1) (TK) (P68)., N = 1, Score = 2222, P = 2.5e-230

SWISSPROT :TKT_RAT TRANSKETOLASE (EC 2.2.1.1) (TK) . , N = 1, Score = 2202, P = 3.3e-228

TREMBL:RN09256_1 product: "transketolase"; Rattus norvegicus Sprague-Dawley transketolase mRNA, complete eds., N = 1, Score = 2202, P = 3.3e-228

SWISSPROT :TKT_HUMAN TRANSKETOLASE (EC 2.2.1.1) (TK) . , N = 1, Score = 2200, P = 5.3e-228

>SWISSPROT:TKT_MOUSE TRANSKETOLASE (EC 2.2.1.1) (TK) (P68). Length = 623

HSPs:

Score = 2222 (333.4 bits), Expect = 2.5e-230, P = 2.5e-230 Identities = 417/614 (67%), Positives = 501/614 (81%)

Query: 7 KPDVKTVQVLRDTANRLRIHSIRATCASGSGQLTSCCSAAEVVSVLFFHTMKYKQTDPEH 66 KPD + +Q L+DTANRLRI SI+AT A+GSG TSCCSAAE+++VLFFHTM+YK DP + Sbjct: 6 KPDQQKLQALKDTANRLRISSIQATTAAGSGHPTSCCSAAEIMAVLFFHTMRYKALDPRN 65

Query: 67 PDNDRFILSRGHAAPILYAAWVEVGDISESDLLNLRKLHSDLERHPTPRLPFVDVATGSL 126

P NDRF+LS+GHAAPILYA W E G + E++LLNLRK+ SDL+ HP P+ F DVATGSL Sbjct: 66 PHNDRFVLSKGHAAPILYAVWAEAGFLPEAELLNLRKISSDLDGHPVPKQAFTDVATGSL 125

Query: 127 GQGLGTACGMAYTGKYLDKASYRVFCLMGDGESSEGSVWEAFAFASHYNLDNLVAVFDVN 186

GQGLG ACGMAYTGKY DKASYRV+C++GDGE SEGSVWEA AFA Y LDNLVA+FD+N Sbjct: 126 GQGLGAACGMAYTGKYFDKASYRVYCMLGDGEVSEGSVWEAMAFAGIYKLDNLVAIFDIN 185

Query: 187 RLGQSGPAPLEHGADIYQNCCEAFGWNTYLVDGHDVEALCQAFWQASQVKNKPTAIVAKT 246

RLGQS PAPL+H DIYQ CEAFGW+T +VDGH VE LC+AF QA K++PTAI+AKT Sbjct: 186 RLGQSDPAPLQHQVDIYQKRCEAFGWHTIIVDGHSVEELCKAFGQA KHQPTAIIAKT 242

Query: 247 FKGRGIPNIEDAENWHGKPVPKERADAIVKLIESQIQTNENLIPKSPVEDSPQISITDIK 306

FKGRGI IED E WHGKP+PK A+ I++ I SQ+Q+ + ++ P ED+P + I +1+ Sbjct: 243 FKGRGITGIEDKEAWHGKPLPKNMAEQIIQEIYSQVQSKKKILATPPQEDAPSVDIANIR 302

Query: 307 MTSPPAYKVGDKIATQKTYGLALAKLGRANERVIVLSGDTMNSTFSEIFRKEHPERFIEC 366

M +PP+YKVGDKIAT+K YGLALAKLG A++R+I L GDT NSTFSE+F+KEHP+RFIEC Sbjct: 303 MPTPPSYKVGDKIATRKAYGLALAKLGHASDRIIALDGDTKNSTFSELFKKEHPDRFIEC 362

Query: 367 IIAEQNMVSVALGCATRGRTIAFAGAFAAFFTRAFDQLRMGAISQANINLIGSHCGVSTG 426

IAEQNMVS+A+GCATR RT+ F FAAFFTRAFDQ+RM AIS++NINL GSHCGVS G Sbjct: 363 YIAEQNMVSIAVGCATRDRTVPFCSTFAAFFTRAFDQIRMAAISESNINLCGSHCGVSIG 422

Query: 427 EDGVSQMALEDLAMFRSIPNCTVFYPSDAISTEHAIYLAANTKGMCFIRTSQPETAVIYT 486

EDG SQMALEDLAMFRS+P TVFYPSD ++TE A+ LAANTKG+CFIRTS+PE A+IY+ Sbjct: 423 EDGPSQMALEDLAMFRSVPMSTVFYPSDGVATEKAVELAANTKGICFIRTSRPENAIIYS 482

Query: 487 PQENFEIGQAKVVRHGVNDKVTVIGAGVTLHEALEAADHLSQQGISVRVIDPFTIKPLDA 546

E+F++GQAKVV +D+VTVIGAGVTLHEAL AA+ L + IS+RV+DPFTIKPLD Sbjct: 483 NNEDFQVGQAKVVLKSKDDQVTVIGAGVTLHEALAAAESLKKDKISIRVLDPFTIKPLDR 542

Query: 547 ATIISSAKATGGRVITVEDHYREGGIGEAVCAAVSREPDILVHQLAVSGVPQRGKTSELL 606

1+ SA+AT GR++TVEDHY EGGIGEAV AAV EP + V +LAVS VP+ GK +ELL Sbjct: 543 KLILDSARATKGRILTVEDHYYEGGIGEAVSAAVVGEPGVTVTRLAVSQVPRSGKPAELL 602

Query: 607 DMFGISTRHIIAAV 620

MFGI 1+ AV Sbjct: 603 KMFGIDKDAIVQAV 616

Pedant information for DKFZphtes3_17117, frame 1

Report for DKFZphtes3_17117.1

[LENGTH] 626

[MW] 67877.52

[pi] 5.90

[HOMOL] SWISSPROT :TKT_MOUSE TRANSKETOLASE (EC 2.2.1.1) (TK) (P68) . 0.0

[FUNCAT] m outer membrane and cell wall [M jannaschn, MJ0681] 3e-48

[FUNCAT] g carbohydrate metabolism and transport [H. influenzae, HI1023] 9e-36

[FUNCAT] 01.05.01 carbohydrate utilization [S. cerevisiae, YPR074c] 5e- -32

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YPR074C] 5e- -32

[FUNCAT] 02.07 pentose-phosphate pathway [S. cerevisiae, YPR074c] 5e- -32

[FUNCAT] 01.01.01 ammo-acid biosynthesis [S. cerevisiae, YPR074C] 5e- -32

[FUNCAT] l lipid metabolism [H. influenzae HI1439] 3e-17

[FUNCAT] c energy conversion [H. influenzae, HI1233] 2e-09

[FUNCAT] 02.01 glycolysis [S. cerevisiae, YBR221C PDB1 - pyruvate dehydrogenase]

2e-05

[FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YBR221c PDB1 - pyruvate dehydrogenase] 2e-05

[BLOCKS] BL00801F

[BLOCKS] BL00801E

[BLOCKS] BL00801D Transketolase proteins

[BLOCKS] BL00801C Transketolase proteins

[BLOCKS] BL00801B Transketolase proteins

[BLOCKS] BL00801A Transketolase proteins

[SCOP] dltrka2 3.28.1.2.1 Transketolase Transketolase, C-terminal domai le-21

[EC] 1.2.4.1 Pyruvate dehydrogenase (lipoamide) 8e-ll

[EC] 1.2.4.4 3-Methyl-2-oxobutanoate dehydrogenase (lipoamide) 4e-10

[EC] 2.2.1.1 Transketolase 0.0

[EC] 2.2.1.3 Formaldehyde transketolase le-20

[PIRKW] transferase 0.0

[PIRKW] flavoprotein 2e-07

[PIRKW] Calvin cycle le-40

[PIRKW] heterotetramer 2e-07 [PIRKW] pentose phosphate pathway 0.0

[PIRKW] magnesium le-40 ,

[PIRKW] thiamine pyrophosphate 0.0

[PIRKW] oxidoreductase 7e-12

[PIRKW] fatty acid biosynthesis 4e-10

[PIRKW] mitochondrion 2e-07

[PIRKW] peroxisome le-20

[PIRKW] homodimer le-40

[SUPFAM] pyruvate dehydrogenase (lipoamide) alpha chain le-06

[SUPFAM] pyruvate dehydrogenase (lipoamide) beta chain 7e-12

[SUPFAM] ferredoxm 2 [4Fe-4S] -related protein 8e-47

[SUPFAM] thiamine pyrophosphate-binding domain homology 0.0

[SUPFAM] pyruvate dehydrogenase (lipoamide) 6e-08

[SUPFAM] ferredoxm 2[4Fe-4S] homology 8e-47

[SUPFAM] hypothetical protein C2814 2e-21

[SUPFAM] transketolase 0.0

[PROSITE] ATP_GTP_A 1

[PFAM] Transketolase

[KW] Alpha_Beta

[KW] 3D

[KW] LOW COMPLEXITY 3.04 %

SEQ MMANDAKPDVKTVQVLRDTANRLRIHSIRATCASGSGQLTSCCSAAEVVΞVLFFHTMKYK SEG IngsB HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH-HHCCCT

SEQ QTDPEHPDNDRFILSRGHAAPILYAAWVEVGDISESDLLNLRKLHSDLERHPTPRLPFVD SEG IngsB TTTTTTTTTCEEEETTGGGHHHHHHHHHHHCTTCHHHHHTTTTTTTTTTTTTTTTTTTTC

SEQ VATGSLGQGLGTACGMAYTGKYLDKASYRVFCLMGDGESSEGSVWEAFAFASHYNLDNLV SEG IngsB CCCCTTTHHHHHHHHHHHHHHHHCBTTBTTEEEECHHHHHCHHHHHHHHHHHHHCTTTEE

SEQ AVFDVNRLGQSGPAPLEHGADIYQNCCEAFGWNTYLVDGHDVEALCQAFWQASQVKNKPT SEG IngsB EEEEECCEETTEEGGGCCCCCHHHHH-HHHCCEEEETTTTTHHHHHHHHHHHHHTTTTCE

SEQ AIVAKTFKGRGIPNIEDAENWHGKPVPKERADAIVKLIESQIQTNENLIPKSPVEDSPQI SEG IngsB EEEEECTTTTTTCCHHHHHHHHHHTCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH

SEQ SITDIKMTSPPAYKVGDKIATQKTYGLALAKLGRANERVIVLSGDTMNSTFSEIFRKEHP SEG IngsB HHHHHHHHHTCCCTTTTCBCHHHHHHHHHHHHHTTTTTEEEEETTTHHHHCCTTCEECCG

SEQ ERFIECIIAEQNMVSVALGCATRGRTIAFAGAFAAFFTRAFDQLRMGAISQANINLIGSH SEG xxxxxxxxxxxxxxxxxxx IngsB GCEEETTTTHHHHHHHHHHHHHHTTTTEEEEEEGGGGGGGHHHHHHHHHHCTTTEEEEEC

SEQ CGVSTGEDGVSQMALEDLAMFRSIPNCTVFYPSDAISTEHAIYLAANTKGMCFIRTSQPE SEG IngsB CCGGGTTTTTTTTCCHHHHHHHCTTTTEEECCCCHHHHHHHHHHHTTTTCEEEECCCCCB

SEQ TAVIYTPQENFEIGQAK VRHGVNDKVTVIGAGVTLHEALEAADHLSQQGISVRVIDPFT SEG IngsB CCTTTTCHHHHHCC-CEEEETTTTTTEEEEECCHHHHHHHHHHHHHHHHCCCEEEE....

SEQ IKPLDAATIISSAKATGGRVITVEDHYREGGIGEAVCAAVSREPDILVHQLAVSGVPQRG SEG IngsB

SEQ KTSELLDMFGISTRHIIAAVTLTLMK SEG IngsB

Prosite for DKFZphtes3_17117.1

PS00017 595->603 ATP GTP A PDOC00017

Pfam for DKFZphtes3_17117.1

HMM_NAME Trans etolase

HMM *vNtIRιLaMDAVEKANSGHPGaPMGMAPMAHVLWqrMMRHNPNDPrWPN +N++RI ++ A + +SG ++++++A++ VL++++M+++++DP P+ Query 20 ANRLRIHSIRATCASGSGQLTSCCSAAEVVSVLFFHTMKYKQTDPEHPD 68

HMM RDRFVLSNGHaCMLLYsMWHLyGYDMpMWDLkQFRQWHSrTPGHPEIgHT

+DRF+LΞ GHA+++LY+ W + G ++++DL+++R++HS++ +HP ++ Query 69 NDRFILSRGHAAPILYAAWVEVGD-ISESDLLNLRKLHSDLERHPTPRLP 117

HMM PGVEVTTGPLGQGIaNaVWMAIAERnLAATYNRPGFDIfDHYTYCFMGDG ++ +V+TG+LGQG++ +++++Y++++ D+++++++C+MGDG

Query 118 FV-DVATGSLGQGLG TACGMAYTGKYLDKASYRVFCLMGDG 157

HMM CLMEGISWEACSLAGHMqLGNWIaFYDDNrlSIDGdTdlWFqEDtYakRF

+ +EG++WEA ++A+H++L+N++A +D NR++++G++++ + D+Y+ + Query 158 ESSEGSVWEAFAFASHYNLDNLVAVFDVNRLGQSGPAPLEHGADIYQNCC 207

HMM EAYGWHVIEVEnDGHDvEelcaAIEeAKaekDRPTLIiCRTVIGYGSPNk

EA+GW++ +V DGHDVE++C A+ +A +K++PT+I ++T++G+G+PN Query 208 EAFGWNTYLV—DGHDVEALCQAFWQASQVKNKPTAIVAKTFKGRGIPNI 255

HMM QGTHdWHGAPLGeD*

++ + WHG+P +++ Query 256 EDAENWHGKPVPKE 269

HMM *PqWePnddkIATRKASQqaLeaiGPaLPEfWGGSADLTPSNLTrWKGmv

P++++ +DKIAT K+++ AL+++G A +++ +S+D+ +S++++++ ++ Query 311 PAYKV-GDKIATQKTYGLALAKLGRANERVIVLSGDTMNSTFSEIFRKE 358

HMM WFMPPSISTDCynGNWsGRYIHYGIREHgMgAIMNGIAIHGgNFRPYGGT

+ + R+I++ I+E++M++++ G+A++G+ ++++ G

Query 359 H PERFIECIIAEQNMVSVALGCATRGR-TIAFAGA 392

HMM FMMFyDYARPAIRMAALMelPVIWVWTHDSIGLGEDGPTHQPVEHLAHFR

F++F+++A++++RM A++ +++++++H++++ GEDG +++++E+LA+FR Query 393 FAAFFTRAFDQLRMGAISQANINLIGSHCGVSTGEDGVSQMALEDLAMFR 442

HMM alPNMsVWRPCDgNETayAWylAvERehTPtiLILSRQNLPQIErNPrqf

+IPN +V++P+D+ T+ A YLA+++++ +++++S ++ +++++ P + Query 443 SIPNCTVFYPSDAISTEHAIYLAANTKGM-CFIRTΞQPETAVIYT-PQEN 490

HMM ekvaRGGYVLkDmdnePDVILIATGSEMELAvaAAKlLadEGIkaRVVSM

++++++++V + + + V++I++G+++++A++AA+ L+ +GI +RV+++ Query 491 FEIGQAKVVRHGVN—DKVTVIGAGVTLHEALEAADHLSQQGISVRVIDP 538

HMM PCTeWFD kQDeEYReSVLPDhVPqRVaVEmGvtWCWYKYVGqq

++++++D + ++++R +++DH++ +++++++V ++ +++ + Query 539 FTIKPLDAATIISSAKATGGRVITVEDHYR-EGGIGEAVCAAVSREPDIL 587

HMM GalfGMNrFGESSGKAPpevLYkMFGFTPENI*

+ +++ +++ ++ +L+ MFG+ +1 Query 588 VHQLAVSGVPQR GKTSELLDMFGISTRHI 616

DKFZphtes3_17nl2

group: transcription factors

DKFZphtes3_17nl2.1 encodes a novel 804 amino acid protein which is nearly identical to mouse and trout SOX-LZ .

Sox proteins belong to the HMG box superfamily of DNA-bindmg proteins and are involved in the regulation of developmental processes as germ layer formation, organ development and cell type specification. Deletion or mutation of Sox proteins often results in developmental defects and congenital disease in humans. Sox proteins perform their function m a complex interplay with other transcription factors in a manner highly dependent on cell type and promoter context. The new protein is related to the SOX-LZ protein and contains an additional leucin-zipper .

The new protein can find application in modulating/blocking the expression of SOX-controlled genes . nearly identical to mouse SOX-LZ complete cDNA, complete eds, few EST hits mouse and trout SOX-LZ, involved in spermatogenesis

Sequenced by GBF

Locus: unknown

Insert length: 2802 bp

Poly A stretch at pos. 2692, polyadenylation signal at pos. 2660

1 GGGATAGGAA AGATGAAAGG TCATGGTGAG CTTCAAGGAC ATGAAAGGTT

51 GTTGTCTCAT GTAACAATAG TAGATTGTTT TTTTTCCTAA TATTTCTAGC

101 CAGCCCCTAA GTCAGGTGAT GGAACAAATA CCTACAGTTT AGTCAGGTGA

151 AACAGGAGTG GGTGGAGGAA GGAAAGAAGA AAAATGGGAA GAATGTCTTC

201 CAAGCAAGCC ACCTCTCCAT TTGCCTGTGC AGCTGATGGA GAGGATGCAA

251 TGACCCAGGA TTTAACCTCA AGGGAAAAGG AAGAGGGCAG TGATCAACAT

301 GTGGCCTCCC ATCTGCCTCT GCACCCCATA ATGCACAACA AACCTCACTC

351 TGAGGAGCTA CCAACACTTG TCAGTACCAT TCAACAAGAT GCTGACTGGG

401 ACAGCGTTCT GTCATCTCAG CAAAGAATGG AATCAGAGAA TAATAAGTTA

451 TGTTCCCTAT ATTCCTTCCG AAATACCTCT ACCTCACCAC ATAAGCCTGA

501 CGAAGGGAGT CGGGACCGTG AGATAATGAC CAGTGTTACT TTTGGAACCC

551 CAGAGCGCCG CAAAGGGAGT CTTGCCGATG TGGTGGACAC ACTGAAACAG

601 AAGAAGCTTG AGGAAATGAC TCGGACTGAA CAAGAGGATT CCTCCTGCAT

651 GGAAAAACTA CTTTCAAAAG ATTGGAAGGA AAAAATGGAA AGACTAAATA

701 CCAGTGAACT TCTTGGAGAA ATTAAAGGTA CACCTGAGAG CCTGGCAGAA

751 AAAGAACGGC AGCTCTCCAC CATGATTACC CAGCTGATCA GTTTACGGGA

801 GCAGCTACTG GCAGCGCATG ATGAACAGAA AAAACTGGCA GCGTCACAAA

851 TTGAGAAACA ACGGCAGCAA ATGGACCTTG CTCGCCAACA GCAAGAACAG

901 ATTGCGAGAC AACAGCAGCA ACTTCTGCAA CAGCAGCACA AAATTAATCT

951 CCTGCAGCAA CAGATCCAGG TTCAGGGTCA CATGCCTCCG CTCATGATCC

1001 CAATTTTTCC ACATGACCAG CGGACTCTGG CAGCAGCTGC TGCTGCCCAA

1051 CAGGGATTCC TCTTCCCCCC TGGAATAACA TACAAACCAG GTGATAACTA

1101 CCCCGTACAG TTCATTCCAT CAACAATGGC AGCTGCTGCT GCTTCTGGAC

1151 TCAGCCCTTT ACAGCTCCAG CAGCTCTATG CCGCTCAGCT GGCCAGCATG

1201 CAGGTGTCAC CTGGAGCAAA GATGCCATCA ACTCCACAGC CACCAAACAC

1251 AGCAGGGACG GTCTCACCTA CTGGGATAAA AAATGAAAAG AGAGGGACCA

1301 GCCCTGTAAC TCAAGTTAAG GATGAAGCAG CAGCACAGCC TCTGAATCTC

1351 TCATCCCGAC CCAAGACAGC AGAGCCTGTA AAGTCCCCAA CGTCTCCCAC

1401 CCAGAACCTC TTCCCAGCCA GCAAAACCAG CCCTGTCAAT CTGCCAAACA

1451 AAAGCAGCAT CCCTAGCCCC ATTGGAGGAA GCCTGGGAAG AGGATCCTCT

1501 TTAGGTAAAT GGAAAAGTCA ACACCAGGAA GAGACTTACG AATTAGATAT

1551 CCTATCTAGT CTCAACTCCC CTGCCCTTTT TGGGGATCAG GATACAGTGA

1601 TGAAAGCCAT TCAGGAGGCG CGGAAGATGC GAGAGCAGAT CCAGCGGGAG

1651 CAACAGCAGC AACAGCCACA TGGTGTTGAC GGGAAACTGT CCTCCATAAA

1701 TAATATGGGG CTGAACAGCT GCAGGAATGA AAAGGAAAGA ACGCGCTTTG

1751 AGAATTTGGG GCCCCAGTTA ACGGGAAAGT CAAATGAAGA TGGAAAACTG

1801 GGCCCAGGTG TCATCGACCT TACTCGGCCA GAAGATGCAG AGGGAAGTAA

1851 AGCAATGAAT GGCTCTGCAG CTAAACTACA GCAGTATTAT TGTTGGCCAA

1901 CAGGAGGTGC CACTGTGGCT GAAGCACGAG TCTACAGGGA CGCCCGCGGC

1951 CGTGCCAGCA GCGAGCCACA CATTAAGCGA CCAATGAATG CATTCATGGT

2001 TTGGGCAAAG GATGAGAGGA GAAAAATCCT TCAGGCCTTC CCCGACATGC

2051 ATAACTCCAA CATTAGCAAA ATCTTAGGAT CTCGCTGGAA ATCAATGTCC

2101 AACCAGGAGA AGCAACCTTA TTATGAAGAG CAGGCCCGGC TAAGCAAGAT

2151 CCACTTAGAG AAGTACCCAA ACTATAAATA CAAACCCCGA CCGAAACGCA

2201 CCTGCATTGT TGATGGCAAA AAGCTTCGGA TTGGGGAGTA TAAGCAACTG

2251 ATGAGGTCTC GGAGACAGGA GATGAGGCAG TTCTTTACTG TGGGGCAACA

2301 GCCTCAGATT CCAATCACCA CAGGAACAGG TGTTGTGTAT CCTGGTGCTA

2351 TCACTATGGC AACTACCACA CCATCGCCTC AGATGACATC TGACTGCTCT 2401 AGCACCTCGG CCAGCCCGGA GCCCAGCCTC CCGGTCATCC AGAGCACTTA

2451 TGGTATGAAG ACAGATGGCG GAAGCCTAGC TGGAAATGAA ATGATCAATG

2501 GAGAGGATGA AATGGAAATG TATGATGACT ATGAAGATGA CCCCAAATCA

2551 GACTATAGCA GTGAAAATGA AGCCCCGGAG GCTGTCAGTG CCAACTGAGG

2601 AGTTTTTGTT TGCTGAATTA AAGTACTCTG ACATTTCACC CCCCTCCCCA

2651 ACAAAGAGTT ATTAAAGAGC CCGCATGCAT TTGTGGCTCC ACAATTAAAA

2701 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

2751 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

2801 AA

BLAST Results

NO BLAST result

Medline entries

95311974:

A gene that is related to SRY and is expressed in the testes encodes a leucine zipper-containing protein.

96032826:

The Sry-related HMG box-containing gene Sox6 is expressed in the adult testis and developing nervous system of the mouse.

Peptide information for frame 1

ORF from 184 bp to 2595 bp; peptide length: 804 Category: strong similarity to known protein

1 MGRMSSKQAT SPFACAADGE DAMTQDLTSR EKEEGSDQHV ASHLPLHPIM 51 HNKPHSEELP TLVSTIQQDA DWDΞVLSSQQ RMESENNKLC SLYSFRNTST 101 SPHKPDEGSR DREIMTSVTF GTPERRKGSL ADVVDTLKQK KLEEMTRTEQ 151 EDSSCMEKLL SKDWKEKMER LNTSELLGEI KGTPESLAEK ERQLSTMITQ 201 LISLREQLLA AHDEQKKLAA SQIEKQRQQM DLARQQQEQI ARQQQQLLQQ 251 QHKINLLQQQ IQVQGHMPPL MIPIFPHDQR TLAAAAAAQQ GFLFPPGITY 301 KPGDNYPVQF IPSTMAAAAA SGLSPLQLQQ LYAAQLASMQ VSPGAKMPST 351 PQPPNTAGTV SPTGIKNEKR GTSPVTQVKD EAAAQPLNLS ΞRPKTAEPVK 401 SPTSPTQNLF PAΞKTSPVNL PNKSSIPSPI GGSLGRGSSL GKWKSQHQEE 451 TYELDILSSL NSPALFGDQD TVMKAIQEAR KMREQIQREQ QQQQPHGVDG 501 KLSSINNMGL NSCRNEKERT RFENLGPQLT GKSNEDGKLG PGVIDLTRPE 551 DAEGSKAMNG SAAKLQQYYC WPTGGATVAE ARVYRDARGR ASSEPHIKRP 601 MNAFMVWAKD ERRKILQAFP DMHNSNISKI LGSRWKSMSN QEKQPYYEEQ 651 ARLSKIHLEK YPNYKYKPRP KRTCIVDGKK LRIGEYKQLM RSRRQEMRQF 701 FTVGQQPQIP ITTGTGVVYP GAITMATTTP SPQMTSDCSS TSASPEPSLP 751 VIQSTYGMKT DGGSLAGNEM INGEDEMEMY DDYEDDPKSD YSSENEAPEA 801 VSAN

BLASTP hits

Entry MMSOXLZ2_l from database TREMBL: product: "SOX-LZ"; Mouse mRNA for SOX-LZ, complete eds.

Score = 3910, P = O.Oe+00, identities = 764/801, positives = 774/801

Entry 151083 from database PIR:

SOX-LZ - rainbow trout

Score = 1774, P = l.le-287, identities = 365/532, positives = 431/532

Entry S59121 from database PIR:

SOX6 protein - mouse

Score = 2319, P = 1.2e-240, identities = 489/660, positives = 527/660

Entry AB006330_1 from database TREMBL: gene: "mSox5L"; product: "SOX5"; Mus musculus mSox5L mRNA, complete eds .

Score = 1212, P = 8.9e-209, identities = 274/457, positives = 324/457

Entry MMU010604_1 from database TREMBL: gene: "sox5"; product: "L-Sox5 protein"; Mus musculus mRNA for transcription factor L-Sox5

Score = 879, P = 4.2e-195, identities = 190/281, positives = 218/281 Alert BLASTP hits for DKFZphtes3_17nl2, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphtes3_17nl2, frame 1

Report for DKFZphtes3_17nl2.1

[LENGTH] 804 [MW] 89332.69 [pi] 6.97 [HOMOL] TREMBL :MMSOXLZ2 1 product: "SOX-LZ"; Mouse mRNA for SOX-LZ, complete eds. 0.0

[FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YKL032c] 8e-07

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKL032c] 8e-07

[FUNCAT] 01.07.07 regulation of vitamins, cofactors, and prosthetic groups [S. cerevisiae, YPR065W] 5e-06

[FUNCAT] 03.04 budding, cell polarity and filament formation [Ξ. cerevisiae, YBR089c-a]

7e-06

[FUNCAT] 30.13 organization of chromosome structure [S. cerevisiae, YBR089c-a] 7e-06

[FUNCAT] 03.01 cell growth [S. cerevisiae, YBR089c-a] 7e-06

[FUNCAT] 03.16 dna synthesis and replication [S. cerevisiae, YMR072w] 2e-04

[FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YMR072w] 2e-04

[SCOP] dlhmf 1.20.1.1.1 HMG1, fragments A and B [rat/hamster (Rattu le-13

[SCOP] dllefa_ 1.20.1.1.6 Lymphoid enhancer-binding factor, LEFl [mous 4e-15

[SCOP] dlhrya_ 1.20.1.1.4 SRY [Human (Homo sapiens) le-11

[PIRKW] DNA binding 4e-94

[PIRKW] T-cell receptor 4e-07

[PIRKW] leucine zipper le-38

[PIRKW] alternative splicing 2e-07

[PIRKW] transcription factor 4e-16

[PIRKW] transcription regulation le-12

[SUPFAM] HMG box homology 0.0

[SUPFAM] unassigned HMG box proteins 4e-94

[PROSITE] ATP_GTP_A 1

[PROSITE] LEUCINE_ZIPPER 1

[PROSITE] MYRISTYL 6

[PROSITE] AMIDATION 1

[PROSITE] CAMP_PHOSPHO_SITE 2

[PROSITE] CK2_PHOSPHO_SITE 14

[PROSITE] PKC_PHOSPHO_SITE 10

[PROSITE] ASN_GLYCOSYLATION 6

[PFAM] HMG (high mobility group) box

[KW] Irregular

[KW] 3D

[KW] LOW_COMPLEXITY 13.81 %

[KW] COILED COIL 3.48 %

SEQ MGRMSSKQATSPFACAADGEDAMTQDLTSREKEEGSDQHVASHLPLHPIMHNKPHSEELP SEG COILS lnhm-

SEQ TLVSTIQQDADWDSVLSSQQRMESENNKLCSLYSFRNTSTSPHKPDEGSRDREIMTSVTF SEG COILS lnhm-

SEQ GTPERRKGSLADVVDTLKQKKLEEMTRTEQEDSSCMEKLLSKDWKEKMERLNTSELLGEI SEG COILS lnhm-

SEQ KGTPESLAEKERQLSTMITQLISLREQLLAAHDEQKKLAASQIEKQRQQMDLARQQQEQI SEG xxxxxxxxxxxxxxx COILS CCCCCC lnhm-

SEQ ARQQQQLLQQQHKINLLQQQIQVQGHMPPLMIPIFPHDQRTLAAAAAAQQGFLFPPGITY SEG xxxxxxxxxxxxxxxxxxxxxx xxxxxx COILS CCCCCCCCCCCCCCCCCCCCCC lnhm-

SEQ KPGDNYPVQFIPSTMAAAAASGLSPLQLQQLYAAQLASMQVSPGAKMPSTPQPPNTAGTV SEG xxxxxxxxxxxx COILS lnhm-

SEQ SPTGIKNEKRGTSPVTQVKDEAAAQPLNLSSRPKTAEPVKSPTSPTQNLFPASKTSPVNL

SEG

COILS lnhm-

SEQ PNKSSIPSPIGGSLGRGSSLGKWKSQHQEETYELDILSSLNSPALFGDQDTVMKAIQEAR

SEG ... xxxxxxxxxxxxxxxxxx

COILS lnhm-

SEQ KMREQIQREQQQQQPHGVDGKLSSINNMGLNSCRNEKERTRFENLGPQLTGKSNEDGKLG

SEG .. xxxxxxxxxxxx

COILS lnhm-

SEQ PGVIDLTRPEDAEGSKAMNGSAAKLQQYYCWPTGGATVAEARVYRDARGRASΞEPHIKRP

SEG

COILS lnhm- CCC

SEQ MNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKSMSNQEKQPYYEEQARLSKIHLEK

SEG X

COILS lnhm- CCCHHHHHHHHHHHHHHHTTTTCCHHHHHHHHHHHTTTTTTHHHHHHHHHHHHHHHHHHH

SEQ YPNYKYKPRPKRTCIVDGKKLRIGEYKQLMRSRRQEMRQFFTVGQQPQIPITTGTGVVYP

SEG xxxxxxxxxxxx

COILS lnhm- HHHTTTTTTT

SEQ GAITMATTTPSPQMTSDCSSTSASPEPSLPVIQΞTYGMKTDGGSLAGNEMINGEDEMEMY

SEG xxxxxxx

COILS lnhm-

SEQ DDYEDDPKSDYΞSENEAPEAVSAN

SEG xxxxxx

COILS lnhm-

Prosite for DKFZphtes3_17nl2.1

PS00001 97->101 ASN_GLYCOSYLATION PDOC00001 PS00001 172-M76 ASN_GLYCOSYLATION PDOC00001 PS00001 388->392 ASN_GLYCOSYLATION PDOC00001 PS00001 422->426 ASN_GLYCOSYLATION PDOC00001 PS00001 559->563 ASN_GLYCOSYLATION PDOC00001 PΞ00001 626->630 ASN_GLYCOSYLATION PDOC00001 PS00004 126->130 CAMP_PHOSPHO_SITE PDOC00004 PS00004 369->373 CAMP_PHOSPHO_ΞITE PDOC00004 PS00005 5->8 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 28->31 PKC_PHOSPHO_SITE PDOC00005 PS00005 94->97 PKC_PHOSPHO_SITE PDOC00005 PS00005 136->139 PKC_PHOSPHO_SITE PDOC00005 PS00005 203->206 PKC_PHOSPHO_SITE PDOC00005 PS00005 299->302 PKC_PHOSPHO_SITE PDOC00005 PS00005 390->393 PKC_PHOSPHO_SITE PDOC00005 PS00005 512->515 PKC_PHOSPHO_SITE PDOC00005 PS00005 530->533 PKC_PHOSPHO_SITE PDOC00005 PS00005 692->695 PKC_PHOSPHO_SITE PDOC00005 PS00006 28->32 CK2_PHOSPHO_SITE PDOC00006 PS00006 129->133 CK2_PHOSPHO_SITE PDOC00006 PS00006 146->150 CK2_PHOSPHO_SITE PDOC00006 PS00006 148->152 CK2_PHOSPHO_SITE PDOC00006 PS00006 154->158 CK2_PHOSPHO_SITE PDOC00006 PS00006 186->190 CK2_PHOSPHO_SITE PDOC00006 PS00006 203->207 CK2_PHOSPHO_SITE PDOC00006 PS00006 221->225 CK2_PHOSPHO_SITE PDOC00006 PS00006 520->524 CK2_PHOSPHO_SITE PDOC00006 PS00006 533->537 CK2_PHOSPHO_SITE PDOC00006 PS00006 547->551 CK2_PHOSPHO_SITE PDOC00006 PS00006 577->581 CK2_PHOSPHO_SITE PDOC00006 PS00006 639->643 CK2_PHOSPHO_SITE PDOC00006 PS00006 793->797 CK2_PHOSPHO_SITE PDOC00006 PS00008 182->188 MYRISTYL PDOC00008 PS00008 431->437 MYRISTYL PDOC00008 PS00008 437->443 MYRISTYL PDOC00008 PS00008 509->515 MYRISTYL PDOC00008 PS00008 575->581 MYRISTYL PDOC00008 PS00008 762->768 MYRISTYL PDOC00008 PS00009 677->681 AMIDATION PDOC00009 PS00017 526->534 ATP_GTP_A PDOC00017 PS00029 187->209 LEUCINE ZIPPER PDOC00029

Pfam for DKFZphtes3_17nl2.1

HMM_NAME HMG (high mobility group) box

HMM *PKRPMNAYMLWMQEMRekIKaENPNdMhNtEISKMιGEMWKnMsEEEKm +KRPMNA+M+W+++ R+KI + P DMHN++ISK++G +WK+MS +EK+

Query 597 IKRPMNAFMVWAKDERRKILQAFP-DMHNSNISKILGSRWKSMSNQEKQ 644

HMM PYEdMAeeEKqRYMKEMPeYK* PY+++ +++ + +++ +P+YK

Query 645 PYYEEQARLSKIHLEKYPNYK 665

DKFZphtes3 17nl8

group: intracellular transport and trafficking

DKFZphtes3_17nl8 encodes a novel 782 amino acid protein with weak partial similarity to known proteins .

The novel protein contains a ATP/GTP-binding site motif A (P-loop) and a TonB-dependent receptor protein signature 1. In E. coli, the tonB protein interacts with outer membrane receptor proteins that mediate uptake of specific substrates into the periplasmic space. In the absence of tonB these receptors bind their substrates but do not carry out active transport. The novel protein seems to be involved m ATP-dependent transport of substances into the cell.

The new protein can find application in modulation of cell-permeability and transport of suitable substrates into the cell. unknown receptor protein containes TONB_DEPENDENT_REC_l Pattern and ATP_GTP_A Pattern,

Sequenced by GBF

Locus: unknown

Insert length: 2853 bp

Poly A stretch at pos. 2806, no polyadenylation signal found

1 GTCCTTTTAA GTCAGTAAAT TGAACTAAGT CGGTTATTCG GCAAGCAGTT

51 CCTATAAAAA ACTACATGGC TAAGGTTCTT AATGATTGAC CACAAGCAGA

101 TCTTTCACCC TCGGATCTCT AGCTACAAAA GGTCCCCACA CTGAAGAAGC

151 CACTACCTCC ACCACCACCA GCACCACCAC GTCCAGTGCT GCTGGCAACC

201 ACTGGGGCAG CCAAGCGCTC CACCCTCTCT CCCACCATGG CCCGTCAGGT

251 GCGCACCCAC CAGGAGACCC TGAACAGGTT TCAGCAGCAG TCCATCCACC

301 TGCTGACGGA GCTCCTCAGA CTGAAGATGA AGGCCATGGT GGAGTCTATG

351 TCGGTGGGTG CCAACCCCTT GGACATCACC AGGCGCTTTG TGGAGGCCAG

401 CCAGCTCCTC CACCTCAATG CCAAGGAGAT GGCCTTCAAC TGCCTGATCA

451 GCACAGCCGG GAGAAGTGGC TACAGCAGCG GACAGTTGTG GAAAGAGTCC

501 CTCGCAAACA TGTCCGCCAT TGGGGTGAAC TCGCCTTACC AGCTGATCTA

551 CCACTCTTCC ACAGCCTGTC TGAGCTTTTC TCTCTCTGCT GGAAAAGAAG

601 CCAAGAAGAA AATAGGCAAA TCTAGAACTA CAGAAGATGT CAGCATGCCG

651 CCCCTGCATC GAGGAGTGGG AACCCCTGCC AACAGCCTGG AGTTCAGCGA

701 CCCCTGCCCT GAGGCCCGGG AGAAGCTGCA GGAGTTGTGT CGCCACATAG

751 AAGCTGAAAG GGCCACATGG AAAGGGAGGA ATATCTCCTA CCCCATGATC

801 TTACGAAACT ACAAGGCAAA GATGCCCTCT CATCTAATGT TGGCCCGCAA

851 AGGAGACTCT CAGACCCCGG GTTTACATTA CCCTCCCACT GCAGGTGCTC

901 AGACTCTCAG CCCCACCTCT CACCCATCTT CTGCCAACCA TCATTTCAGT

951 CAGCATTGTC AAGAGGGGAA GGCACCCAAG AAGGCCTTCA AGTTTCATTA

1001 CACCTTCTAT GATGGCTCCT CCTTCGTTTA CTATCCCTCT GGAAACGTCG

1051 CTGTATGTCA GATCCCCACA TGCTGCAGAG GGAGAACCAT CACCTGCCTC

1101 TTTAATGACA TACCTGGATT CTCCTTGCTG GCCCTATTCA ATACTGAAGG

1151 CCAGGGCTGT GTTCACTACA ACCTAAAAAC CAGTTGCCCA TATGTCTTAA

1201 TCTTGGATGA GGAAGGTGGG ACCACCAATG ACCAGCAGGG CTATGTAGTC

1251 CACAAGTGGA GCTGGACTTC CAGGACAGAG ACCCTGCTTT CCCTGGAATA

1301 CAAGGTGAAT GAGGAAATGA AACTAAAGGT ACTGGGACAG GACTCCATCA

1351 CAGTCACCTT CACCTCCCTG AATGAGACAG TAACACTCAC TGTGTCGGCC

1401 AACAATTGTC CCCATGGAAT GGCATATGAC AAACGGCTGA ACCGCAGAAT

1451 CAGCAACATG GACGACAAGG TGTATAAGAT GAGCCGAGCC CTGGCTGAGA

1501 TCAAGAAGCG GTTTCAGAAG ACAGTGACTC AGTTCATTAA TTCTATCTTG

1551 CTGGCCGCAG GTCTGTTTAC CATTGAATAT CCCACCAAAA AGGAGGAGGA

1601 AGAATTTGTT CGGTTCAAGA TGAGATCCAG AACTCATCCC GAGCGGCTCC

1651 CCAAGCTAAG TTTATACTCA GGAGAAAGTC TTTTACGATC TCAGTCAGGC

1701 CACCTGGAAT CCTCAATTGC AGAGACTTTG AAGGATGAGC CTGAGTCTGC

1751 TCCTGTGAGC CCAGTTCGGA AGACCACCAA AATCCACACC AAAGCCAAGG

1801 TCACATCCAG AGGGAAGGCC CGCGAGGGGC GCAGCCCCAC CAGGTGGGCG

1851 GCCTTGCCCT CAGACTGCCC GCTGGTGCTG CGGAAGCTCA TGCTCAAGGA

1901 AGACACCCGT GCTGGCTGCA AGTGCCTGGT GAAGGCGCCC CTGGTCTCTG

1951 ACGTGGAGCT GGAGCGCTTC CTGTTGGCGC CCCGAGACCC CAGCCAAGTG

2001 CTGGTGTTTG GGATCATCTC AAGCCAGAAC TACACCAGCA CTGGGCAGCT

2051 CCAGTGGCTG CTGAACACTC TCTACAACCA CCAGCAGCGG GGCCGTGGCT

2101 CCCCCTGCAT CCAGTGCCGG TATGACTCCT ACCGCCTGCT GCAGTATGAC

2151 CTGGACAGCC CCCTGCAGGA GGACCCTCCC CTGATGGTGA AGAAGAACTC

2201 TGTGGTGCAG GGGATGATTC TGATGTTTGC CGGGGGGAAG CTCATTTTTG

2251 GGGGCCGTGT TTTGAATGGA TATGGCCTCA GCAAGCAGAA TCTGCTGAAA

2301 CAGATCTTCC GGTCTCAACA GGATTACAAG ATGGGCTACT TCCTGCCGGA

2351 TGACTACAAA TTCAGTGTTC CCAACTCTGT CCTGAGCCTG GAGGATTCTG

2401 AATCAGTCAA GAAAGCCGAG TCAGAAGATA TCCAAGGAAG CAGCTCCTCA 2451 TTGGCCCTGG AAGACTATGT GGAtJAAGGAG TTATCTCTGG AGGCTGAGAA

2501 GACAAGAGAG CCTGAAGTGG AGCTACATCC TCTCAGCAGG GACAGCAAGA

2551 TAACTAGTTG GAAGAAGCAG GCCTfJtAAGA AGTAGCGCCA TCCTGGCAGC

2601 AGCCAAGTGA GCCAGGCCCC GGCCCGGGGT GCTGGGGCTT CTTGCCAGCC

2651 CAGCCCTGCC TCCCCGGTCT CCCACCCTGΓ CCTCCAAGCT TCTATAATAA

2701 ACCAGCGGGC CTCCAGCATT GGGGTGAGGC TCTGGGGAAG GACAAAAAAA

2751 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAGGG

2801 CGGCCGAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAGGGCGG

2851 CCG

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 237 bp to 2582 bp; peptide length: 782

Category: putative protein

Prosite motifs: ATP_GTP_A (122-130)

TONB DEPENDENT REC 1 (1-44)

1 MARQVRTHQE TLNRFQQQSI HLLTELLRLK MKAMVESMΞV GANPLDITRR

51 FVEASQLLHL NAKEMAFNCL ISTAGRSGYS SGQLWKESLA NMSAIGVNSP

101 YQLIYHSSTA CLSFSLSAGK EAKKKIGKSR TTEDVSMPPL HRGVGTPANS

151 LEFSDPCPEA REKLQELCRH IEAERATWKG RNISYPMILR NYKAKMPSHL

201 MLARKGDSQT PGLHYPPTAG AQTLSPTSHP SSANHHFSQH CQEGKAPKKA

251 FKFHYTFYDG SSFVYYPSGN VAVCQIPTCC RGRTITCLFN DIPGFSLLAL

301 FNTEGQGCVH YNLKTSCPYV LILDEEGGTT NDQQGYVVHK WSWTSRTETL

351 LSLEYKVNEE MKLKVLGQDS ITVTFTSLNE TVTLTVSANN CPHGMAYDKR

401 LNRRISNMDD KVYKMSRALA EIKKRFQKTV TQFINSILLA AGLFTIEYPT

451 KKEEEEFVRF KMRSRTHPER LPKLΞLYSGE SLLRΞQSGHL ESSIAETLKD

501 EPESAPVSPV RKTTKIHTKA KVTSRGKARE GRSPTRWAAL PSDCPLVLRK

551 LMLKEDTRAG CKCLVKAPLV SDVELERFLL APRDPSQVLV FGIISSQNYT

601 STGQLQWLLN TLYNHQQRGR GSPCIQCRYD SYRLLQYDLD SPLQEDPPLM

651 VKKNSVVQGM ILMFAGGKLI FGGRVLNGYG LSKQNLLKQI FRSQQDYKMG

701 YFLPDDYKFS VPNSVLSLED SESVKKAESE DIQGSSSSLA LEDYVEKELS

751 LEAEKTREPE VELHPLSRDS KITSWKKQAS KK

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_17nl8, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphtes3_17nl8, frame 3

Report for DKFZphtes3_17nl8.3

[LENGTH] 782

[MW] 88030.16

[pi] 9.22

[BLOCKS] BL00286 Squash family of serme protease inhibitors proteins

[PROSITE] ATP_GTP_A 1

[PROSITE] MYRISTYL 4

[PROSITE] CAMP_PHOSPHO_SITE 3

[PROSITE] CK2_PHOSPHO_SITE 14

[PROSITE] PROKAR_LIPOPROTEIN 1

[PROSITE] TONB_DEPENDENT_REC_l 1

[PROSITE] PKC_PHOSPHO_ΞITE 10

[PROSITE] ASN_GLYCOSYLATION 4

[KW] Alpha_Beta SEQ MARQVRTHQETLNRFQQQSIHLLTELLRLKMKAMVESMSVGANPLDITRRFVEASQLLHL PRD ccchhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhhh

SEQ NAKEMAFNCLISTAGRSGYSSGQLWKESLANMSAIGVNSPYQLIYHSSTACLSFSLSAGK PRD hhhhhhhhhhhhcccccccccccchhhhhhhhhcccccccceeeecccceeeecccccch

SEQ EAKKKIGKSRTTEDVSMPPLHRGVGTPANSLEFSDPCPEAREKLQELCRHIEAERATWKG PRD hhhhhhhcccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhc

SEQ RNISYPMILRNYKAKMPSHLMLARKGDΞQTPGLHYPPTAGAQTLSPTSHPSSANHHFSQH PRD cccccchhhhhhhhcccccceeeccccccccccccccccccccccccccccccccccccc

SEQ CQEGKAPKKAFKFHYTFYDGSSFVYYPSGNVAVCQIPTCCRGRTITCLFNDIPGFSLLAL PRD ccccccchhhhheeeecccccceeeecccceeeeeccccccceeeeeeccccccceeeee

SEQ FNTEGQGCVHYNLKTSCPYVLILDEEGGTTNDQQGYVVHKWSWTSRTETLLSLEYKVNEE PRD ecccccceeeeeccccccceeeeecccccccccceeeeeeecccchhhhhhhhhhhhhhh

SEQ MKLKVLGQDSITVTFTSLNETVTLTVSANNCPHGMAYDKRLNRRISNMDDKVYKMSRALA PRD hhhhhhccceeeeeeccccceeeeeeecccccccchhhhhhhhhhhcccchhhhhhhhhh

SEQ EIKKRFQKTVTQFINSILLAAGLFTIEYPTKKEEEEFVRFKMRSRTHPERLPKLSLYSGE PRD hhhhhhhhhhhhhhhhhhhhcccceeecccchhhhhhhhhhhccccccccccceeeeccc

SEQ SLLRSQSGHLESSIAETLKDEPESAPVSPVRKTTKIHTKAKVTSRGKAREGRSPTRWAAL PRD eeeecccccchhhhhhhhhccccccccccccccccccceeeeeccccccccccccccccc

SEQ PSDCPLVLRKLMLKEDTRAGCKCLVKAPLVSDVELERFLLAPRDPSQVLVFGIISSQNYT PRD ccccchhhhhhhhhhhhhhhhhhhhccccchhhhhhhhhccccccceeeeeeeecccccc

SEQ STGQLQWLLNTLYNHQQRGRGSPCIQCRYDSYRLLQYDLDSPLQEDPPLMVKKNSVVQGM

PRD ccchhhhhhhhhhhhhcccccccceeeecccccceeecccccccccccccccccchhhhh

SEQ ILMFAGGKLIFGGRVLNGYGLSKQNLLKQIFRSQQDYKMGYFLPDDYKFSVPNSVLSLED PRD heeeccccccccccccccccccchhhhhhhhhhhhhccccccccccceeecccceeeccc

SEQ SESVKKAESEDIQGSSSSLALEDYVEKELSLEAEKTREPEVELHPLSRDSKITSWKKQAS PRD chhhhhhhhcccccccccchhhhhhhhhhhhhhhhhcccceeeccccccccccccccccc

SEQ KK PRD cc

Prosite for DKFZphtes3_17nl8.3

PS00001 91->95 ASN_GLYCOSYLATION PDOC00001 PS00001 182->186 ASN_GLYCOSYLATION PDOC00001 PS00001 379->383 ASN_GLYCOSYLATION PDOC00001 PS00001 598->602 ASN_GLYCOSYLATION PDOC00001 PS00004 403->407 CAMP_PHOSPHO_SITE PDOC00004 PS00004 511->515 CAMP_PHOSPHO_SITE PDOC00004 PS00004 652->656 CAMP_PHOSPHO_SITE PDOC00004 PS00005 48->51 PKC_PHOSPHO_SITE PDOC00005 PS00005 177->180 PKC_PHOSPHO_SITE PDOC00005 PS00005 344->347 PKC_PHOSPHO_SITE PDOC00005 PS00005 450->453 PKC_PHOSPHO_SITE PDOC00005 PS00005 497->500 PKC_PHOSPHO_SITE PDOC00005 PS00005 513->516 PKC_PHOSPHO_SITE PDOC00005 PS00005 523->526 PKC_PHOSPHO_SITE PDOC00005 PS00005 631->634 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 723->726 PKC_PHOSPHO_SITE PDOC00005 PS00005 774->777 PKC_PHOSPHO_SITE PDOC00005 PS00006 7->ll CK2_PHOSPHO_SITE PDOC00006 PS00006 131->135 CK2_PHOSPHO_SITE PDOC00006 PS00006 256->260 CK2_PHOSPHO_SITE PDOC00006 PS00006 329->333 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 345->349 CK2_PHOSPHO_SITE PDOC00006 PS00006 377->381 CK2_PHOSPHO_SITE PDOC00006 PS00006 406->410 CK2_PHOSPHO_SITE PDOC00006 PS00006 450->454 CK2_PHOSPHO_SITE PDOC00006 PS00006 466->470 CK2_PHOSPHO_SITE PDOC00006 PS00006 493->497 CK2_PHOSPHO_SITE PDOC00006 PS00006 497->501 CK2_PHOSPHO_SITE PDOC00006 PS00006 571->575 CK2_PHOSPHO_SITE PDOC00006 PS00006 693->697 CK2_PHOΞPHO_SITE PDOC00006 PS00006 717->721 CK2_PHOSPHO_SITE PDOC00006 PS00008 145->151 MYRISTYL PDOC00008 PS00008 327->333 MYRISTYL PDOC00008 PS00008 592->598 MYRISTYL PDOC00008 PS00008 734->740 MYRISTYL PDOC00008 PS00013 101->112 PROKAR_LIPOPROTEIN PDOC00013 PS00017 122->130 ATP_GTP_A PDOC00017

PS00430 l->44 TONB DEPENDENT REC 1 PDOC00354

(No Pfam data available for DKFZphtes3_17nl8.3)

DKFZphtes3_18f3

group: testes derived

DKFZphtes3_18f3 encodes a novel 248 amino acid protein with partial similarity to human TNF- mducible protein CG12-1.

The novel protein contains two leucine zippers.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . similarity to TNF-mducible protein CG12-1

Sequenced by MediGenomix

Locus : unknown

Insert length: 4608 bp

Poly A stretch at pos. 4570, polyadenylation signal at pos. 4550

1 GACAGAAGTG AATGGGAATG GAGAGGCCGG CGGCCCGGGA GCCGCATGGG

51 CCCGACGCGC TGCGGCGCTT CCAGGGACTG CTGCTGGACC GCCGAGGCCG

101 GCTGCACCGC CAGGTGCTGC GCCTGCGCGA GGTGGCCCGG CGCCTGGAGC

151 GCCTGCGCAG GCGCTCCCTC GTAGCCAACG TGGCCGGCAG CTCGCTGAGC

201 GCAACGGGCG CCCTCGCCGC CATCGTGGGG CTCTCGCTCA GCCCGGTCAC

251 CCTGGGGACC TCGCTGCTGG TGTCGGCCGT GGGGCTGGGG GTGGCCACAG

301 CCGGAGGGGC CGTCACCATC ACGTCCGATC TCTCGCTGAT CTTCTGCAAC

351 TCCCGGGAGC TGCGGAGGGT GCAGGAGATC GCGGCCACCT GCCAGGACCA

401 GATGCGAGAG ATCCTGAGCT GCCTCGAGTT TTTCTGCCGC TGGCAGGGCT

451 GCGGGGACCG CCAGCTGCTG CAGTGCGGGA GGAACGCCTC CATCGCCCTG

501 TACAATTCTG TCTACTTCAT CGTCTTCTTT GGCTCACGTG GCTTCCTCAT

551 CCCCAGGCGG GCGGAGGGGG ACACCAAGGT TAGCCAGGCC GTGCTGAAGG

601 CCAAGATTCA GAAACTGGCC GAGAGCCTGG AGTCCTGCAC CGGGGCTCTG

651 GACGAACTCA GCGAGCAGCT GGAGTCTCGG GTTCAGCTCT GCACCAAGTC

701 CAGTCGTGGC CACGACCTCA AGATCTCTGC TGACCAGCGT GCAGGGCTGT

751 TTTTCTGAGA ACATCCTTTC CCCCTAATGA CCGAGGCCAG CAAATCATCC

801 TCATGGGATG CTCCAGAATT TGTAGCTCCC TTAGGAAAAC ACCAAGCTGG

851 GTTAGGAGCC GAAGGCAAAG GATGAGAAAA ACTGTTTTTG AAGTGGGCAG

901 GTCCCCAAAG CCCTTCTTTT CCCATCACTG TGACATCTGC CTGGGCTTGA

951 GTGCTACGGA CTTTTCAGTC TTCCTAGTGG AAAAATGTGA CCCAAAAACT

1001 CCTTTTCCTT TATCAAAAAC TTTCTGTCTA AACACAGCTG GGCAGGCACT

1051 CCTGTTTTAA AGTTATTTCG GGGTCCCTGA CCCTGCCCTG GTGGCTTGGC

1101 CTGAGACTGG AGAGAGTGCC ATCCTCTGGG TCCTCTCCAA GTCCTACTAG

1151 TCTTTGAAGT CCTCAAAATG TGCGTGAGGA AGGCATTTGC CTCTATTCCA

1201 GAATTTCTGA TACAAAGAAC TCCAGAATCC AGAGCAAATC AGCCCTTCTC

1251 TGAACGTTGT AGGATGGTTC AGAACCCAGA GAGGACCCTG GTGCTGATAT

1301 CTCCTCCTCT TCCCTTTCCC CTCAGCTTAC TTACTCCCAG ATGCGGCCTG

1351 GGTATGAAGT AGGCCTTTCC TGAGTGGCTC CCAATCCAGT CCTCCAAGTA

1401 CTCAGAGGGG AAGCCCGTGA AGCCGTCATC TAAGTCCTGC TCCCTCACAT

1451 GAAGCTGAGG GCCAGATAGA TGGAGCGACT GCCAACTTCA TTTCCCGACA

1501 TCATTGTGTT CAGAAGAGAG TGATGGGTTT TGAGTTAGAC AGTCCTGGGC

1551 TTGAGACAGG CTTTGTCACT ACTGTGTGAG TGTAGCCACC TAATCTCTCT

1601 GAGACTGTGT AAAACAAAGA TGATAAAATC TCACCCTGTT GTGAGATATT

1651 AAATGAGCCA AAGTGCCTAG CATGATGGTG CTGGCTCATA TAGTGTAGTC

1701 CCTGGAATGG CAAATTAACA TCACCCAGGA ACTTGTTAGA AAGGCAAATT

1751 CTTGGACACA ACCCTCCTGA TTTATGGAAT CAGAAACTCT GGCTGTGGGG

1801 CCCAGCAACC TGAGTTTAAA CAATTTCTCT GGGTGGTTCT GCGGCACACT

1851 AAGGTTTGAA AATCACTACA ACAAATGCTA ACTTCTAATC CCCTTGATGA

1901 GCTTTCACGA AGTCTCACGG CTTCTCTAGG GACTCCATGG TCTTCAGAGT

1951 CGTTCACAGA TGACCAAGGA CAGACTGTGT CCCAGAAGCC AAAATGAGAG

2001 AGAGAGAGAG AGCACGCGTA CGTGCACCCT GGGGCAGTGT CTCACCGTAT

2051 GAATAAGGGA TGTAACACTA AAAGCCCATT AGGGGGCAGT GTTTCCCGCC

2101 TGTTGTAGAA ACTGGTACAG AAAGGATCCT ATATGAAGTT CCTGAAACTG

2151 ACCTTTGTCT ATTATTACCT TCTCTGAAAA GTGCCAGTCC ATGTATTTTT

2201 TATTTATTTT AAGTTTGTAA TTTAATTTTT AATTATTGTT TAGTGTTTGC

2251 ATTTAATTTT ATTTAATCAC CACATTTAGA AAATAATAAG AGCAAGTTTC

2301 TAAATGGGAG ACTGCTGAGG CTCTTTGCAA GAGATGAGAT TAAGTTTGAG

2351 TTTCTAAGGC AGGGCATGAG CTGGAAATAG CATTGCTTTC CTTGATTGTC

2401 TCTCTCCTTC AGGGAGATTC TTTTTCTCTA GTGTTTTAAG TGATCCTTTG

2451 AAGTAAGTGT GGAGAGTCTT GAATGGCAAG ACCAGGAGCT GAGTTTAAGC

2501 TTGTAATGGA AGCTTGCATT GTGGGATATA TAACTGAGGA AGCATATTTA

2551 TCCTGAAGGT ATTTTGCCAG AAGGTATCAC TTGACCTGGA AAAGGAATCT

2601 ATTTAGTTCA GGAAAGATAA AAAGTTTAGA GGTATGTGAA GGAAGCACTT

2651 AGAACTTGCA AGCCTGATGT CCTATCAAGT TATGTCTTCT GGGTGACAGA

2701 CAAAATAGCT TGTCTTATGG TGGTGATGTG TTGCATTTTC ACTTTGGGGT 2751 CTGTAAGAAA CTGTCAGTGA AAATATGTAC AATTCCTTCA ATTTCCATTC

2801 TTAACAACTG TAATGTTGAA AAATAAGTTG AAAAGTCTTT GGGACCATAC

2851 ATGCAAAAAC GGTGCCTCTG TTACTTAATT ATTTAATATT CTATAAATGT

2901 ACCCAATCTG TCCGCACCCT TCCCAGTGAT GGGGCAGTAT GTCTGAGGAA

2951 GTATAATTTC AGTACTGGGG TCGGGGAGAG GAGGTGATGT TTCTACATTT

3001 TTATTTTTTC TATAAATTGC AATTGGTCTG TATGCTGGTT TATTTTGAAA

3051 TTTATATTGG TTTCTTTTCA AGCTGGTGTC ATCTCCTAGA CTGTTTCACC

3101 CAGATGCTAG CATTTTTTTT TTTTTTGAGA CAGAGTCTCA CTCTGTCACC

3151 TAGGCTGGAG TTGCAGTGGT TTGATCTCGG CTCACTGCAA CCTCCGACTC

3201 CTGGGTTCAA GCAATTCTTC TGCCTCAGCC TCCTGAGTAG CTGGGATTAC

3251 AGATGTGCAC CAGCACACCC GGCTAATTTT TTGTATTTTT AGTAGAGACA

3301 GGGTTTCGCC ATGTTGGCCA GGCTGGTCTT GAACTCCTGG CCTTATGTGA

3351 TCCGCCCACC TTGGCTTCCC AAAGTGCTGG GATTACAGGC ATGAGCCACC

3401 TCGCCTGGCC AGATGCTAGC ATTTTAGATC AAACAATTCA TTTTAGATGA

3451 ATTGTTTTGT TTCACAATCA TTTTAAATCA TTTTAGAATG TACTTCACAT

3501 TATTAGTTGT GTTATGGCAT AAAGGTACAA CCATTCCCTA ACTCCATCTT

3551 TTATTAATGC TTAAGTTTAA ATTATATTCT TCCAATGCCT AAGCTATTCC

3601 CTAGAATTAA ACTGGGCACT TTTGGAAGCA GCAACAGTAA CAGCAGCAGC

3651 AAACTTTTCC TCTCATATTT TGGGTGTATC AAAAGTTCTA GACTTTTGAA

3701 GTTATGATTT CAGTGGCCCA CTTTATTTCT AAGGAAGAGT GTCTACTTTG

3751 GAACGATACT TTGCACATAG TAGGAACTCA AGAAATACAT TTGAATAATT

3801 ATAATTAACT GTTTAGCTAT CTTAATGAGA ATTTGTTGAC AACAAAAGAT

3851 CATCCATCGC CTTATGTGTG AGTAAGATTG GAGCCTCTAT CAAGATTTAG

3901 TCAAGTTCAG TTAGATTGAT TCTAGAAACA AATATTTATT TCTTTCTTTT

3951 ACGGGGATGT GAATAAGGCT TTTCCTTAAG GCCTTCATTC TTTAAACAAA

4001 CAGGTTGAAA TGGTATGTTG TAAAAGAGAA GACGGGAGAG AGGTATTTAG

4051 ATGATAAGTG TACTTCACAA AAATGCCAAA GTTTGAAAAA TAGGTATGTT

4101 TGTTCTAAAT GTTTAAGTGC TTCTCTGTTA GGTTCTGGGG CTTGCAATCA

4151 TTTGAATTGT TCTGTTTCAC AATAAAGGAG ATTCACTGGG TTCTGCATTT

4201 TCAGGATTCA ATAGAACTGC TCCATTAAAA AAATAATCCT TAGCAAGCAT

4251 TCGAATCCTA ACTGCTTTGA TGCACTTGCC CTCGGGCACC TGTCATTTCC

4301 AATATGGTAG GTGTCAAAGT CAAAAGTATT TACTGGGAGA AAAAAGAGAG

4351 GAGTGGTTGT AGAAGTCTCC CTAAATCAGA CATGTCAAGC AATCAGCCAA

4401 CGTGGTGTAT TTCTCATTCA ATATTTTAGT GTGAATTGAG ACACTGAGAT

4451 AAAGACATCG TGCAGAGATA AATGGGGATA CAGTTAAATG TAGCAACTCT

4501 TGAGTTCATT TTTTCCCACT GTAGCAAAAT TAATGCTTTC TCTTTATTGA

4551 AATAAATTGC TCATTCCTCC AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

4601 AAAAAAGG

BLAST Results

Entry HSG27587 from database EMBL: human STS SHGC-32548. Score = 1951, P = 9.0e-101, identities = 411/425

Entry HS073350 from database EMBL: human STS EST303564. Score = 1417, P = 8.7e-58, identities = 285/287

Medline entries

No Medline entry

Peptide information for frame 2

ORF from the beginning to 580 bp; peptide length: 194 Category: questionable ORF Classification: no clue

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_18f3, frame 2

PIR:CGB01S collagen alpha 1(1) chain - bovme (fragments), N = 1, Score = 155, P = 4.5e-10

TREMBL :HSCG1PA1_1 gene: "COL1A1"; Human proalpha 1 (I) chain of type I procollagen mRNA (partial)., N = 1, Score = 155, P = 6.5e-10 >PIR : CGB01S collagen alpha 1 ( 1 ) chain - bovine ( fragments ) Length = 779

HSPs :

Score = 155 (23.3 bits), Expect = 4.5e-10, P = 4.5e-10 Identities = 60/152 (39%), Positives = 67/152 (44%)

Query: 7 GEAGGPGAAWARRAAALPGTAA—GPPRPAAPPGA--APARGGPAPGAPAQALPRSQRGR 62

G+ G PG + AR PG GPP PA P GA AP G A A P SQ Sbjct: 230 GDLGAPGPSGARGERGFPGERGVEGPPGPAGPRGANGAPGNDGAKGDAGAPGAPGSQGAP 289

Query: 63 QLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGAAE 122

L G P RGA PG GD +GA G + G VR L + PG A Sbjct: 290 GL QGMPGE-RGAAGLPGPKGDRGDAGPKGADGAPGKDG VRGLTGPIGPPGPAG 341

Query: 123 GAGDRGHL-P-GP DARDPELPRVFLPLAGLRGPPAA 156

GD+G P GP D +P P P AG GPP A Sbjct: 342 APGDKGEAGPSGPAGTRGAPGDRGEPGPPG P-AGFAGPPGA 381

Score = 121 (18.2 bits), Expect = 5.4e-05, P = 5.4e-05 Identities = 52/154 (33%), Positives = 60/154 (38%)

Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARG GPAPGAPAQALPRSQRG 61

G G PGAA R P AGPP P P G ++G GPA G P + P G Sbjct: 434 GATGFPGAA-GRVGPPGPSGNAGPPGPPGPAGKEGSKGPRGETGPA-GRPGEVGPPGPPG 491

Query: 62 RQLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGAA 121

A G P G PG PG RG G +RG R L PG + Sbjct: 492 P—AGEKGAPGAD-GPAGAPGTPGPQGIAGQRGVVGLPGQRGE RGFPGL PGPS 541

Query: 122 EGAGDRGHLPGPDARDPELPRVFLPLAGLRGPPAAAVRE 160

G +G R P P + GL GPP + RE Sbjct: 542 GEPGKQGPSGASGERGPPGP MGPPGLAGPPGESGRE 577

Score = 117 (17.6 bits), Expect = 1.8e-04, P = 1.8e-04 Identities = 52/148 (35%), Positives = 62/148 (41%)

Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAA PPGAAPARGGPAPGAPAQALPRSQRG-R 62

G G PG AR +A PG A G P A PPG + GP PG P A +G R Sbjct: 416 GNVGAPGPKGARGSAGPPG-ATGFPGAAGRVGPPGPS-GNAGP-PGPPGPAGKEGSKGPR 472

Query: 63 QLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRH--HHVRSLADLLQLPGA 120

GRP G + PG PG GA G G + ++ LPG Sbjct: 473 GETGPAGRP GEVGPPGPPGPAGEKGAPGADGPAGAPGTPGPQGIAGQRGVVGLPGQ 528

Query: 121 AEGAGDRGH--LPGPDARDPEL-PRVFLPLAGLRGPP 154

G+RG LPGP + P +G RGPP Sbjct: 529 R GERGFPGLPGPSGEPGKQGPS GASGERGPP 559

Score = 117 (17.6 bits), Expect = 1.8e-04, P = 1.8e-04 Identities = 54/162 (33%), Positives = 64/162 (39%)

Query: 7 GEAGGPGAAWARRAAALPGT—AAGPPRPAAPPGAAPARG—GPA—PGAPAQALPRSQR 60

G G PG + PG A+GP P PPG G G A PG P + P + Sbjct: 29 GPPGAPGPQGFQGPPGEPGEPGASGPMGPRGPPGPPGKNGDDGEAGKPGRPGERGPPGPQ 88

Query: 61 G-RQLAERNGRP—RRHRGALAQPGHPGDLAAGVGRGAGGGHΞRRGRHHHV—RSLADLL 115

G R L G P + HRG G GD +G G G + R L Sbjct: 89 GARGLPGTAGLPGMKGHRGFSGLDGAKGDAGPAGPKGEPGSPGENGAPGQMGPRGLPGFP 148

Query: 116 QLPGAA--EG-AGDRGHLPGPDARDPELPRVFLPLAGLRGPPAAA 157

GAA G AG+RG +PGP P AG +GPP A Sbjct: 149 GPKGAAGEPGKAGERG-VPGPPGAVG--PAGKDGEAGAQGPPGPA 190

Score = 113 (17.0 bits), Expect = 5.4e-04, P = 5.4e-04 Identities = 54/148 (36%), Positives = 58/148 (39%)

Query: 7 GEAGGPGAAWARRAAALPGTA AGPPRPAAP PGAAPARGGPAP-GAPAQALPR 57

G AG PGA A PG A AGPP PA P PG G P P GA A P Sbjct: 374 GFAGPPGADGQPGAKGEPGDAGAKGDAGPPGPAGPAGPPGPIGNVGAPGPKGARGSAGPP 433

Query: 58 ΞQRGRQLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQL 117

G A P G PG PG +G G GR V Sbjct: 434 GATGFPGAAGRVGPPGPSGNAGPPGPPGPAGKEGSKGPRGETGPAGRPGEVGP 486

Query: 118 PGAAEGAGDRGHLPGPD—ARDPELPRVFLPLAGLRG 152

PG AG++G PG D A P P +AG RG Sbjct: 487 PGPPGPAGEKG-APGADGPAGAPGTPGP-QGIAGQRG 521

Score = 110 (16.5 bits), Expect = 1.3e-03, P = 1.2e-03 Identities = 54/151 (35%), Positives = 60/151 (39%)

Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAAPPG—AAPAR-GGPAP-GAPAQALPRSQRGR 62

GE G G A + LPG A GPP A PG P G P P GA + +RG Sbjct: 194 GERGEQGPAGSPGFQGLPGPA-GPPGEAGKPGEQGVPGDLGAPGPSGARGERGFPGERGV 252

Query: 63 QLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRΞLADLLQLPGAAE 122

+ PR GA G GD A G+ G +G R A L PG Sbjct: 253 EGPPGPAGPRGANGAPGNDGAKGDAGAPGAPGSQGAPGLQGMPGE-RGAAGL PGPK- 307

Query: 123 GAGDRGHLPGPDARD—PELPRVFLPLAGLRGPPAAA 157

GDRG GP D P V L G GPP A Sbjct: 308 —GDRGDA-GPKGADGAPGKDGV-RGLTGPIGPPGPA 340

Score = 109 (16.4 bits), Expect = 1.7e-03, P = 1.7e-03 Identities = 55/154 (35%), Positives = 60/154 (38%)

Query: 4 NGN-GEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARG-GPAPGAPAQALPRSQRG 61

NG+ GEAG PG R P A G P A PG RG GA A P +G Sbjct: 67 NGDDGEAGKPGRP-GERGPPGPQGARGLPGTAGLPGMKGHRGFSGLDGAKGDAGPAGPKG 125

Query: 62 RQLAE-RNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRΞL ADLL 115

+ NG P + G PG PG A G G G V A Sbjct: 126 EPGSPGENGAPGQ-MGPRGLPGFPGPKGAAGEPGKAGERGVPGPPGAVGPAGKDGEAGAQ 184

Query: 116 QLPGAAEGAGDRGHLPGPDARDPELPRVFLPLAGLRGPPAAA 157

PG A AG+RG GP A P F L G GPP A Sbjct: 185 GPPGPAGPAGERGE-QGP-AGΞPG FQGLPGPAGPPGEA 220

Score = 104 (15.6 bits), Expect = 6.6e-03, P = 6.6e-03 Identities = 44/131 (33%), Positives = 49/131 (37%)

Query: 2 EVNGNGEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARGGPAP-GAPAQALPRSQR 60

E GE G PG R LPG GP A PG A RG P P GA A + Sbjct: 126 EPGSPGENGAPGQMGPR GLPGFP-GPKGAAGEPGKAGERGVPGPPGAVGPAGKDGEA 181

Query: 61 GRQLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGA 120

G Q P RG G PG G+ G G G+ DL PG Sbjct: 182 GAQGPPGPAGPAGERGEQGPAGSPG--FQGLP-GPAGPPGEAGKPGEQGVPGDL-GAPGP 237

Query: 121 AEGAGDRGHLPG 132

+ G+RG PG Sbjct: 238 SGARGERG-FPG 248

Score = 104 (15.6 bits), Expect = 6.6e-03, P = 6.6e-03 Identities = 43/131 (32%), Positives = 55/131 (41%)

Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARGGPAPGAPAQALPRSQRGRQLAE 66

GEAG G A R A PG G P P P G A GP PGA Q + + G A+ Sbjct: 347 GEAGPSGPAGTRGA PGDR-GEPGPPGPAGFA GP-PGADGQPGAKGEPGDAGAK 397

Query: 67 RNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGAAEGAGD 126

+ P G PG G++ A +GA G G + A + PG + AG Sbjct: 398 GDAGPPGPAGPAGPPGPIGNVGAPGPKGARGSAGPPGATGFPGA-AGRVGPPGPΞGNAGP 456

Query: 127 RGHLPGPDARD 137

G PGP ++ Sbjct: 457 PGP-PGPAGKE 466

Score = 104 (15.6 bits). Expect = 6.6e-03, P = 6.6e-03 Identities = 56/162 (34%), Positives = 62/162 (38%)

Query: 7 GEAGGPGAAWARRAAALPGTAA—GPPRPAAPPGAAPARGGPAPGAPAQALPRSQRGRQL 64

G G PGA A G GP P P G A ARG P P Q PR +G Sbjct: 608 GPPGAPGAPGPVGPAGKSGDRGETGPAGPIGPVGPAGARG PAGP-QG-PRGBKGZTG 662

Query: 65 AERNGRPRRHRG ALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLA-DLLQ-LPG 119

+ + + HRG PG PG GA G RG S D L LPG Sbjct: 663 ZZGBRGIKGHRGFSGLQGPPGPPGSPGEQGPSGASGPAGPRGPPGSAGSPGKDGLNGLPG 722

Query: 120 AAEGAGDRGHL—PGPDARDPELPRVFLPLAGLRGPPAAAVREERLHRPVQ 168

G RG GP A P P P G GPP+ L +P Q Sbjct: 723 PIGPPGPRGRTGDAGP-AGPPGPPG P-PGPPGPPSGGYDLSFLPQPPQ 768

Score = 101 (15.2 bits), Expect = 1.5e-02, P = 1.5e-02 Identities = 49/148 (33%), Positives = 55/148 (37%)

Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARGGPAPGAPA QALPRSQRGR 62

G AG PG A R PG A GP A G A A+G P P PA + P G Sbjct: 152 GAAGEPGKAGERGVPGPPG-AVGP AGKDGEAGAQGPPGPAGPAGERGEQGPAGSPGF 207 Query: 63 QLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGAAE 122

Q P G + G PGDL A G G RG R + PG A Sbjct: 208 QGLPGPAGPPGEAGKPGEQGVPGDLGAP GPSGARGERGFPGE-RGVEGP PGPAG 260

Query: 123 GAGDRGHLPGPDARDPELPRVFLPLAGLRGPP 154

G G PG D + P G +G P Sbjct: 261 PRGANG-APGNDGAKGDAGAPGAP—GSQGAP 289

Score = 100 (15.0 bits), Expect = 1.9e-02, P = 1.9e-02 Identities = 40/130 (30%), Positives = 48/130 (36%)

Query: 7 GEAGGPGAAWARRAAALPG —AAGPPRPAAPPGAAPARG—GPA—PGAPAQALPRSQR 60

G G PG + PG A+GP P PPG G G A PG P + P + Sbjct: 29 GPPGAPGPQGFQGPPGEPGEPGASGPMGPRGPPGPPGKNGDDGEAGKPGRPGERGPPGPQ 88

Query: 61 G-RQLAERNGRP--RRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQL 117

G R L G P + HRG G GD +G G G + L Sbjct: 89 GARGLPGTAGLPGMKGHRGFΞGLDGAKGDAGPAGPKGEPGSPGENGAPGQMGPRG-LPGF 147

Query: 118 PGAAEGAGDRG 128

PG AG+ G Sbjct: 148 PGPKGAAGEPG 158

Score = 99 (14.9 bits), Expect = 2.5e-02, P = 2.5e-02 Identities = 53/156 (33%), Positives = 61/156 (39%)

Query: 7 GEAGGPGAAWARRA AALPGT—AAGPPRPAAPPGAAPARG—GPA PGAPAQAL 55

G G PGA R A PG A G P P P G + RG GPA P PA A Sbjct: 587 GRDGSPGAKGDRGETGPAGAPGPPGAPGAPGPVGPAGKSGDRGETGPAGPIGPVGPAGAR 646

Query: 56 PRSQRGRQLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHV 108

PR +G + + + HRG G PG + +G G G Sbjct: 647 GPAGPQGPRGBKGZTGZZGBRGIKGHRGFSGLQGPPGPPGSPGEQGPSGASGPAGPRGP- 705

Query: 109 RΞLADLLQLPGAAEGAGDRG—HLPGPDARDPELPRVFLPLAGLRGPP 154

PG+A G G LPGP P PR AG GPP Sbjct: 706 PGSAGSPGKDGLNGLPGPIG—PPGPRGRTGDAGPAGPP 742

Score = 98 (14.7 bits), Expect = 3.3e-02, P = 3.3e-02 Identities = 51/158 (32%), Positives = 58/158 (36%)

Query: 7 GEAGGPGAAWARRAAALPGTA AGPPRPAAPPGAAPARGGPAP-GAPAQALPRSQR 60

G G G R AA LPG AGP PG RG P G P A + Sbjct: 287 GAPGLQGMPGERGAAGLPGPKGDRGDAGPKGADGAPGKDGVRGLTGPIGPPGPAGAPGDK 346

Query: 61 GRQLAERNGRPRRHRGA LAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQL 117

G A +G P RGA +PG PG GA G +G + D Sbjct: 347 GE—AGPSG-PAGTRGAPGDRGEPGPPGPAGFAGPPGADGQPGAKGEPGDAGAKGDAGP- 402

Query: 118 PGAAEGAGDRGHLPGPDARDPELPRVFLPLAGLRGPPAAAVR 159

PG A AG G + A P+ R G G P AA R Sbjct: 403 PGPAGPAGPPGPIGNVGAPGPKGARGSAGPPGATGFPGAAGR 444

Score = 96 (14.4 bits), Expect = 5.7e-02, P = 5.5e-02 Identities = 46/152 (30%), Positives = 57/152 (37%)

Query: 6 NGEAGGPGAAWARRAAALPGTAA—GPPRPAAPPGAAPARGGPAPGAPA-QALPRΞQRGR 62

+G G PGA + PG G PA PG A G P P PA ++ R + G Sbjct: 574 SGREGAPGAEGSPGRDGSPGAKGDRGETGPAGAPGPPGAPGAPGPVGPAGKSGDRGETGP 633

Query: 63 QLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGAAE 122

P RG G G+ +G G RG H R + L PG Sbjct: 634 AGPIGPVGPAGARGPAGPQGPRGB KGZTGZZGBRGIKGH-RGFΞGLQGPPGPPG 686

Query: 123 GAGDRGHLPGPDARDPELPRVFLPLAGLRGPPAAA 157

G++G P A P AG RGPP +A

Sbjct: 687 SPGEQG--PS-GASGP AGPRGPPGSA 709

Score = 94 (14.1 bits), Expect = 9.7e-02, P = 9.2e-02 Identities = 45/134 (33%), Positives = 56/134 (41%)

Query: 24 PGTAAGPPRPAAPPGAAPARGGPA-PGAPAQALPRSQRGRQLAERNGRPRRHR—GALAQ 80

P G P P PG +G P PG P + P RG G P ++ G + Sbjct: 21 PSGPRGLPGPPGAPGPQGFQGPPGEPGEPGASGPMGPRGPP GPPGKNGDDGEAGK 75

Query: 81 PGHPGDLAA-GV—GRGAGGGHSRRGRHHHVRSLADLLQLPGAAEGAGDRGH—LPGPDA 135

PG PG+ G RG G G H R + L G A AG +G PG + Sbjct: 76 PGRPGERGPPGPQGARGLPGTAGLPGMKGH-RGFSGLDGAKGDAGPAGPKGEPGSPGENG 134

Query: 136 RDPEL-PRVFLPLAGLRGPPAAA 157 ++ PR LP G GP AA Sbjct: 135 APGQMGPRG-LP—GFPGPKGAA 154

Score = 92 (13.8 bits), Expect = 1.7e-01, P = 1.5e-01 Identities = 52/155 (33%), Positives = 58/155 (37%)

Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARGGP-APGAPAQALPRΞQRGRQLA 65

GEAG G A R A G GPP PA G A G P A G P A + G Sbjct: 347 GEAGPSGPAGTRGAPGDRGEP-GPPGPAGFAGPPGADGQPGAKGEPGDAGAKGDAGPPGP 405

Query: 66 ERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGR--HHHVRSLADLLQLPGAA— 121

P G + PG G + GA G GR A PG A

Sbjct: 406 AGPAGPPGPIGNVGAPGPKGARGSAGPPGATGFPGAAGRVGPPGPSGNAGPPGPPGPAGK 465

Query: 122 EGA-GDRGHLPGPDARDPELPRVFLP-LAGLRGPPAA 156

EG+ G RG GP R E+ P AG +G P A Sbjct: 466 EGSKGPRGET-GPAGRPGEVGPPGPPGPAGEKGAPGA 501

Score = 92 (13.8 bits), Expect = 1.7e-01, P = 1.5e-01 Identities = 51/156 (32%), Positives = 57/156 (36%)

Query: 7 GEAGGPGAAWARRA AALPGT—AAGPPRPAAPPGAAPARGGPAPGAPAQAL-PRSQR 60

G G PGA R A PG A G P P P G + RG P P + P R Sbjct: 587 GRDGSPGAKGDRGETGPAGAPGPPGAPGAPGPVGPAGKSGDRGETGPAGPIGPVGPAGAR 646

Query: 61 GRQLAERNGRPRRHRGALAQPGHPGDLA-AGVG—RGAGGGHSRRGRH—HHVRSLADLL 115

G A G PR +G + G G G +G G G A

Sbjct: 647 GP--AGPQG-PRGBKGZTGZZGBRGIKGHRGFSGLQGPPGPPGSPGEQGPSGASGPAGPR 703

Query: 116 QLPGAAEGAGDRG—HLPGPDARDPELPRVFLPLAGLRGPP 154

PG+A G G LPGP P PR AG GPP Sbjct: 704 GPPGSAGSPGKDGLNGLPGPIG—PPGPRGRTGDAGPAGPP 742

Score = 90 (13.5 bits), Expect = 2.8e-01, P = 2.5e-01 Identities = 45/134 (33%), Positives = 53/134 (39%)

Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARGGPAPGAPAQALPRSQRGRQ-LA 65

G G PG A + A G A P P P G A RG G P Q R +RG L Sbjct: 485 GPPGPPGPAGEKGAPGADGPAGAPGTPG-PQGIAGQRG--VVGLPGQ RGERGFPGLP 538

Query: 66 ERNGRPRRH--RGALAQPGHPGDLA AGV GR-GAGGGHSRRGRHHHVRSLADL 114

+G P + GA + G PG + AG GR GA G GR + D Sbjct: 539 GPSGEPGKQGPSGASGERGPPGPMGPPGLAGPPGESGREGAPGAEGSPGRDGSPGAKGDR 598

Query: 115 LQL-PGAAEGAGDRGHLPGP 133

+ P A G PGP Sbjct: 599 GETGPAGAPGPPGAPGAPGP 618

Score = 83 (12.5 bits), Expect = 1.8e+00, P = 8.₃e-01 Identities = 49/156 (31%), Positives = 56/156 (35%)

Query: 7 GEAGGPGAAWARRAAALPGTAA—GPPRPAAPPGAAPARG—GPAP—GAPAQALPRSQR 60

G+AG GA A + G GPP PA PG G GPA GAP R + Sbjct: 311 GDAGPKGADGAPGKDGVRGLTGPIGPPGPAGAPGDKGEAGPSGPAGTRGAPGD RGEP 367

Query: 61 GRQLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGA 120

G P G G PGD A G G G + ++ PG Sbjct: 368 GPPGPAGFAGPPGADGQPGAKGEPGDAGAKGDAGPPGPAGPAGPPGPIGNVG APGP 423

Query: 121 AEGAGDRGHLPGPDARDPELPRVFLP LAGLRGPPAAAVRE 160

G G PG RV P AG GPP A +E

Sbjct: 424 KGARGSAGP-PGATGFPGAAGRVGPPGPSGNAGPPGPPGPAGKE 466

Score = 82 (12.3 bits). Expect = 2.3e+00, P = 9.0e-01 Identities = 46/148 (31%), Positives = 52/148 (35%)

Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARGGPAPGAPAQALPRSQRGRQLAE 66

G+AG PGA ++ A L G G A PG RG P A P R L Sbjct: 275 GDAGAPGAPGSQGAPGLQGMP-GERGAAGLPGPKGDRGDAGPKG-ADGAPGKDGVRGLTG 332

Query: 67 RNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGAAEGAGD 126

G P G PG G+ G G RG A PGA G Sbjct: 333 PIGPP GPAGAPGDKGEAGPSGPAGTRGAPGDRGEPGPPGP-AGFAGPPGADGQPGA 387

Query: 127 RGHLPGP-DARDPELPRVFLPLAGLRGPP 154

+G PG A+ P P AG GPP Sbjct: 388 KGE-PGDAGAKGDAGPPG—P-AGPAGPP 412

Peptide information for frame 3 ORF from 12 bp to 755 bp; peptide length: 248 Category: similarity to known protein Classification: unset

Prosite motifs: LEUCINE_ZIPPER (17-39) LEUCINE ZIPPER (24-46)

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_18f3, frame 3

TREMBL :AF070675_1 product: "TNF-inducible protein CG12-1"; Homo sapiens TNF-inducible protein CG12-1 mRNA, complete eds., N = 1, Score = 135, P = le-06

TREMBL :HS6802_1 gene: "dJ6802.1"; product: "dJ6802.1"; Homo sapiens DNA sequence from PAC 6802 on chromosome 22. Contains apolipoprotein L, myosin heavy chain, ESTs, CA repeat, STS and GSS., N = 1, Score = 107, P = 0.0023

>TREMBL:AF070675_1 product: "TNF-inducible protein CG12-1"; Homo sapiens TNF-mducible protein CG12-1 mRNA, complete eds. Length = 331

HSPs:

Score = 135 (20.3 bits), Expect = 1.0e-06, P = 1.0e-06 Identities = 30/103 (29%), Positives = 55/103 (53%)

Query: 30 RLHRQVLRLREVARRLERLRRRSLVANVAGSSLSATGALAAIVGLSLSPVTLGTSLLVSA 89

++ + +LR +A +E + R ++NV SS A + ++ GL L+P T GTSL ++A Sbjct: 91 KIQESIEKLRALANGIEEVHRGCTISNVVSSSTGAASGIMSLAGLVLAPFTAGTSLALTA 150

Query: 90 VGLGVATAGGAVTITSDL-SLIFCNSRELRRVQEIAATCQDQMR 132

G+G+ A IT+ + + +S E + AT D+++ Sbjct: 151 AGVGLGAASAVTGITTSIVEHSYTSSAEAE-ASRLTATSIDRLK 193

Pedant information for DKFZphtes3_18f3, frame 2

Report for DKFZphtes3_18f3.2

[LENGTH] 193

[MW] 19708.24

[pi] 11.90

[KW] All_Alpha

[KW] LOW_COMPLEXITY 55.44 %

SEQ TEVNGNGEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARGGPAPGAPAQALPRSQR

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx...

PRD cccccccccccccchhhhhhhhhccccccccccccccccccccccccccccccchhhhhh

SEQ GRQLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGA

SEG xxxxxxxxxxxxxxxxxxxxxx

PRD hhhhhhcccccccccccccccccccccccccccccccccccccccchhhhhhhhhccccc

SEQ AEGAGDRGHLPGPDARDPELPRVFLPLAGLRGPPAAAVREERLHRPVQFCLLHRLLWLTW

SEG xxxxxxxxxxxxx xxxxxxxxxx

PRD ccccccccccccccccccccccccccccccccccchhhhhhhhcccchhhhhhhhhhhhc

SEQ LPHPQAGGGGHQG SEG xxxxxxxxxxxxx PRD ccccccccccccc

(No Prosite data available for DKFZphtes3_18f3.2) (No Pfam data available for DKFZphtes3_18f3.2)

Pedant information for DKFZphtes3_18f3, frame 3 Report for DKFZphtes3_18 f 3 . 3

[LENGTH] 248

[MW] 27162.56

[pi] 9.92

[PROSITE] LEUCINE ZIPPER 2

[KW] TRANSMEMBRANE 1

[KW] LOW COMPLEXITY 30.65 %

[KW] COILED COIL 12.10 %

SEQ MGMERPAAREPHGPDALRRFQGLLLDRRGRLHRQVLRLREVARRLERLRRRΞLVANVAGS

SEG XXXXXXXXXXXXXXXXXX . XXXXXXXXXXXXXXXXXXXX . . XXX

PRD cccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccc

COILS

MEM

SEQ SLSATGALAAIVGLSLΞPVTLGTSLLVΞAVGLGVATAGGAVTITSDLSLIFCNSRELRRV

SEG xxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxx

PRD cchhhhhhhhhhhhcccccccccccccccccceeeeccceeeeeeceeeeecchhhhhhh

COILS

MEM MMMMMMMMMMMMMMMMM

SEQ QEIAATCQDQMREILSCLEFFCRWQGCGDRQLLQCGRNASIALYNSVYFIVFFGSRGFLI

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhcccccchhhhhccccchhhhhcceeeeeecccccccc

COILS

MEM

SEQ PRRAEGDTKVSQAVLKAKIQKLAESLESCTGALDELSEQLESRVQLCTKSSRGHDLKISA

SEG

PRD ccccccccchhhhhhhhhhhhhhhhhhhhhcchhhhhhhhhhhhhhhhcccccceeeehh

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

MEM

SEQ DQRAGLFF

SEG

PRD hhhhhccc

COILS

MEM

Prosite for DKFZphtes3_18f3.3

PS00029 17->39 LEUCINE_ZIPPER PDOC00029 PS00029 24->46 LEUCINE ZIPPER PDOC00029

(No Pfam data available for DKFZphtes3_18f3.3)

DKFZphtes3_1817

group: cell structure and motility

DKFZphtes3_1817 encodes a novel 1050 amino acid protein with weak partial similarity to ankyrins .

The novel protein contains an ATP/GTP-bindmg site motif A (P-loop) and an Ank repeat. Ankyrins are peripheral membrane proteins which interconnect integral proteins with the spectrin-based membrane skeleton. Thus the novel protein seems to be involved in coupling of cyto skeleton and cell membrane.

The new protein can find application in modulation of cyto skeleton-membrane interactions.

similarity to ankyrins

Sequenced by MediGenomix

Locus : unknown

Insert length: 4501 bp

Poly A stretch at pos. 4423, no polyadenylation signal found

1 GATCGCCGCG CGAGGGTGGT GGGCATCGAG GTCCCAGCAG CGGACGAGGG

51 AGGTGCCGCC GTCGCCCAGG ATGGGCTGGG AATGAAGCGA TGTAGCCTTT

101 TAAGAGATTT GCTCTGACCC ATCTGAAGTC CATATGGCTC TGTATGATGA

151 AGACCTCCTG AAAAATCCTT TCTATCTGGC TCTGCAAAAG TGCCGCCCTG

201 ACTTGTGCAG CAAAGTGGCC CAAATCCATG GCATTGTCTT AGTACCCTGC

251 AAAGGAAGCC TGTCGAGCAG CATCCAGTCT ACTTGTCAGT TTGAGTCCTA

301 CATTTTGATA CCTGTGGAAG AGCATTTTCA GACCTTAAAT GGAAAGGATG

351 TCTTTATTCA AGGGAACAGG ATTAAATTAG GAGCTGGTTT TGCCTGTCTT

401 CTCTCAGTGC CCATTCTCTT TGAAGAAACT TTCTACAATG AAAAAGAAGA

451 GAGTTTCAGC ATCCTGTGTA TAGCCCATCC TTTGGAAAAG AGAGAGAGTT

501 CAGAAGAGCC TTTGGCACCC TCAGATCCCT TTTCCCTGAA AACCATTGAA

551 GATGTGAGAG AGTTCTTGGG AAGACACTCC GAGCGATTTG ACAGGAACAT

601 CGCCTCTTTC CATCGAACAT TCCGAGAATG CGAGAGAAAG AGCCTCCGTC

651 ACCACATAGA CTCAGCGAAT GCTCTCTACA CCAAATGCCT CCAGCAGCTT

701 CTGAGGGACT CTCACCTGAA AATGCTCGCC AAGCAGGAGG CCCAGATGAA

751 CCTGATGAAG CAGGCAGTGG AGATATACGT CCATCATGAA ATTTACAACC

801 TGATCTTTAA ATACGTGGGG ACCATGGAGG CAAGTGAGGA TGCGGCCTTT

851 AACAAAATCA CAAGAAGCCT TCAAGATCTT CAGCAGAAAG ATATTGGTGT

901 GAAACCGGAG TTCAGCTTTA ACATACCTCG TGCCAAAAGA GAGCTGGCTC

951 AGCTGAACAA ATGCACCTCC CCACAGCAGA AGCTTGTCTG CTTGCGAAAA

1001 GTGGTGCAGC TCATTACACA GTCTCCAAGC CAGAGAGTGA ACCTGGAGAC

1051 CATGTGTGCT GATGATCTGC TATCAGTCCT GTTATACTTG CTTGTGAAAA

1101 CGGAGATCCC TAATTGGATG GCAAATTTGA GTTACATCAA AAACTTCAGG

1151 TTTAGCAGCT TGGCAAAGGA TGAACTGGGA TACTGCCTGA CCTCATTCGA

1201 AGCTGCCATT GAATATATTC GGCAAGGAAG CCTCTCTGCT AAACCCCCTG

1251 AGTCTGAGGG ATTTGGAGAC AGGCTGTTCC TTAAGCAGAG AATGAGCTTA

1301 CTCTCTCAGA TGACTTCGTC TCCCACCGAC TGCCTGTTTA AGCACATTGC

1351 ATCAGGTAAC CAGAAAGAAG TGGAGAGACT TCTGAGCCAA GAGGACCATG

1401 ATAAAGATAC CGTCCAAAAG ATGTGTCACC CTCTCTGCTT CTGCGATGAC

1451 TGTGAGAAAC TCGTCTCTGG GAGGTTGAAT GATCCCTCAG TTGTCACTCC

1501 ATTCTCCAGA GACGACAGGG GGCACACCCC TCTCCATGTG GCTGCTGTCT

1551 GTGGGCAGGC ATCCCTCATC GACCTCCTGG TTTCCAAGGG CGCCATGGTA

1601 AATGCCACAG ACTACCATGG GGCCACTCCG CTCCACCTGG CCTGTCAGAA

1651 GGGCTACCAG AGCGTGACGC TGCTGCTGCT GCACTACAAG GCCAGCGCGG

1701 AAGTGCAGGA CAACAATGGG AATACGCCAC TCCACCTGGC CTGCACCTAC

1751 GGCCACGAGG ACTGTGTGAA GGCTCTGGTT TACTACGACG TGGAGTCGTG

1801 CAGACTTGAC ATTGGCAATG AGAAAGGAGA CACCCCTCTA CACATTGCTG

1851 CCCGCTGGGG CTACCAAGGC GTCATAGAGA CATTGCTGCA GAACGGAGCG

1901 TCCACCGAGA TCCAGAACAG ACTGAAGGAG ACGCCCCTCA AGTGTGCATT

1951 AAACTCAAAG ATTCTGTCTG TAATGGAAGC CTATCACCTG TCCTTCGAGA

2001 GGAGGCAGAA GTCGTCCGAG GCCCCTGTGC AGTCCCCGCA GCGCTCCGTG

2051 GACTCCATCA GCCAAGAGTC CTCCACTTCC AGCTTCTCCT CCATGTCAGC

2101 CGGCTCAAGG CAGGAGGAGA CCAAGAAGGA CTACAGAGAG GTAGAAAAAC

2151 TTTTGAGAGC AGTTGCTGAT GGAGATCTAG AAATGGTGCG TTACCTGTTG

2201 GAATGGACAG AGGAGGACCT GGAGGATGCG GAGGACACTG TCAGTGCAGC

2251 AGACCCCGAA TTCTGTCACC CGTTGTGCCA GTGCCCCAAG TGTGCCCCAG

2301 CTCAGAAGAG GCTGGCGAAG GTTCCTGCCA GTGGGCTTGG TGTGAACGTG

2351 ACCAGCCAGG ACGGCTCCTC CCCGCTGCAT GTCGCCGCCC TGCACGGCCG

2401 GGCGGACCTC ATCCGCCTCC TGCTGAAGCA CGGGGCCAAC GCAGGTGCCA

2451 GGAACGCAGA CCAAGCCGTC CCGCTCCACC TGGCCTGCCA GCAGGGCCAC

2501 TTTCAGGTGG TGAAGTGTCT GTTAGATTCG AATGCAAAAC CCAATAAGAA

2551 GGACCTCAGT GGAAACACGC CCCTCATTTA CGCCTGCTCC GGTGGCCATC

2601 ACGAGCTTGT GGCACTGCTG CTACAGCACG GGGCCTCCAT TAACGCTTCT

2651 AACAATAAGG GCAACACAGC GCTGCACGAG GCTGTGATTG AAAAGCACGT 2701 CTTCGTGGTA GAGCTGCTTC TGCTCCACGG AGCGTCAGTT CAGGTGCTGA

2751 ACAAGCGGCA GCGCACGGCT GTAGACTGTG CTGAACAGAA TTCAAAAATA

2801 ATGGAATTGC TTCAGGTGGT ACCAAGCTGT GTTGCTTCAT TAGATGATGT

2851 GGCTGAAACT GACCGCAAGG AGTATGTCAC TGTTAAGATC AGGAAAAAAT

2901 GGAACTCAAA ACTGTATGAT CTACCAGATG AGCCTTTTAC AAGACAGTTT

2951 TACTTTGTCC ACTCAGCTGG TCAGTTTAAG GGAAAGACTT CAAGGGAGAT

3001 TATGGCAAGA GATAGAAGTG TCCCTAATTT AACCGAAGGT TCTTTGCATG

3051 AGCCAGGGAG GCAAAGTGTC ACACTGAGAC AGAATAACCT GCCAGCTCAG

3101 AGTGGATCTC ATGCTGCTGA GAAAGGCAAC AGCGACTGGC CAGAGAGGCC

3151 TGGACTGACA CAGACTGGCC CTGGACACAG ACGGATGCTG CGGAGACACA

3201 CGGTAGAGGA TGCGGTCGTG TCCCAGGGCC CGGAGGCTGC TGGCCCCCTC

3251 TCCACTCCCC AAGAGGTTAG TGCTTCCCGG TCCTAACAGG AATGAGGAGT

3301 TGTTGAACCC ACTGCTAGGA AGCAAGGATG CAACAAGATG ATGCTGAGCG

3351 TGAACACATC TGAGAACTAA ATGTGCTTCC ATGAGACTGG CTTGAGAAGT

3401 CTTCAGCACC AAGTTCCTGA AAGCTTTTCT GTGGCAGGAA AGAATGCAAC

3451 AAAAAAGTTA ACCACCACCA TCTCTCTCCT CTTCAAAGCT AATGAATACA

3501 ATTGAAACAG ACAAAAATTC CAGTAGCATC CAGATCCTTA AGCCAGAGGT

3551 GCATGCTTCT TTTTAAGTAT GAGGGTTTGT TGGTCACAGT GGGAGAGGTT

3601 TCACCACCGC ATTCTGACCT CCTCCTCCCA AAAGGTGCTA AACCTCTCTG

3651 ACCTGTGTAC ATTCACAAAC CACAGCTAGA ATTCCTCCAC CTAGGATTAA

3701 GCTGGAGAGA AGTAAGTAAT TTAGGTTTCA TGGTACTGTA GAGGCCAGGC

3751 TGAAATGTCA TATCTGAAGG AAGAAAGCAG CAGCTGGACA ATGTTTCTTT

3801 GCAAAGCAAC ACTCGAACCA AAAGATGCCT CAATCCCATT TTGATATTCA

3851 TTTTAGTGAA AGGATGCATC AGACCTGTTC CACATCATGC ACATGGGAAA

3901 GGGTGGTTAT CATTTTCCTT CTAACAAGTA GGTACAGATA TTCGGTTACT

3951 ACACGTGCAC CTGTAGCAGT ATTTCTAGAA ACATCCCTTT TTGTTGAGAA

4001 CCTCCCTTGA ATGTCTGTCA CACTCACACC TGACGGGATG GTTACTGGAT

4051 TAGAGAGTAG ATTTGGCACA TCTTTTCTTA GTCTTTTGAT TCAAATTCAA

4101 AACTTAACAG CACAAACCAG GTCAGAGTTA CTTTCGGTTA GAATTTATTG

4151 CCATTTATTC CTTTTTATAA ATTTCTATAG ATTATACTGT TATTTTTATG

4201 TTATTGGCCT AGAGCTACAC GTATATGGGT TTGTCCTGAG TCCGTTTTCA

4251 AATGACCTTG TGATAGGGAA ATGGTTTTGT CCATGTTCTT GGAAATACTT

4301 GTGTATGTAC AGAAGGAAGG GAGGGATTAT TTTTCTACAA AGTAATTTAT

4351 GATTTCTAAT TTTCTAATGT GCCTTGGATA TGTGCCAAAT GATGGAAAAG

4401 AAACAGTAAA CTTTATGATT CTTAAAAAAA AAAAAAAAAA AAAAAAAAAA

4451 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAG

4501 G

BLAST Results

No BLAST result

^•Medline entries

No Medlme entry

Peptide information for frame 2

ORF from 134 bp to 3283 bp; peptide length: 1050 Category: similarity to known protein Classification: Cell structure/motility Prosite motifs: ATP GTP A (945-953)

1 MALYDEDLLK NPFYLALQKC RPDLCSKVAQ IHGIVLVPCK GSLSSSIQST 51 CQFESYILIP VEEHFQTLNG KDVFIQGNRI KLGAGFACLL SVPILFEETF 101 YNEKEESFSI LCIAHPLEKR ESSEEPLAPS DPFSLKTIED VREFLGRHSE 151 RFDRNIASFH RTFRECERKS LRHHIDSANA LYTKCLQQLL RDSHLKMLAK 201 QEAQMNLMKQ AVEIYVHHEI YNLIFKYVGT MEASEDAAFN KITRSLQDLQ 251 QKDIGVKPEF SFNIPRAKRE LAQLNKCTSP QQKLVCLRKV VQLITQSPSQ 301 RVNLETMCAD DLLSVLLYLL VKTEIPNWMA NLSYIKNFRF ΞSLAKDELGY 351 CLTSFEAAIE YIRQGSLSAK PPESEGFGDR LFLKQRMSLL SQMTSSPTDC 401 LFKHIASGNQ KEVERLLSQE DHDKDTVQKM CHPLCFCDDC EKLVSGRLND 451 PSVVTPFSRD DRGHTPLHVA AVCGQASLID LLVΞKGAMVN ATDYHGATPL 501 HLACQKGYQS VTLLLLHYKA SAEVQDNNGN TPLHLACTYG HEDCVKALVY 551 YDVESCRLDI GNEKGDTPLH IAARWGYQGV IETLLQNGAS TEIQNRLKET 601 PLKCALNSKI LSVMEAYHLS FERRQKSSEA PVQSPQRSVD SISQESSTSS 651 FSSMSAGSRQ EETKKDYREV EKLLRAVADG DLEMVRYLLE WTEEDLEDAE 701 DTVSAADPEF CHPLCQCPKC APAQKRLAKV PASGLGVNVT SQDGSSPLHV 751 AALHGRADLI RLLLKHGANA GARNADQAVP LHLACQQGHF QVVKCLLDSN 801 AKPNKKDLSG NTPLIYACSG GHHELVALLL QHGASINASN NKGNTALHEA 851 VIEKHVFVVE LLLLHGASVQ VLNKRQRTAV DCAEQNSKIM ELLQVVPSCV 901 ASLDDVAETD RKEYVTVKIR KKWNSKLYDL PDEPFTRQFY FVHSAGQFKG

951 KTSREIMARD RSVPNLTEGS LHEPGRQSVT LRQNNLPAQS GSHAAEKGNS

1001 DWPERPGLTQ TGPGHRRMLR RHTVEDAVVΞ QGPEAAGPLS TPQEVSASRS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_1817, frame 2

TREMBL:HSU43965_1 gene: "ANK3"; product: "ankyrin G119"; Human ankyrin G119 (ANK3) mRNA, complete ds., N = 2, Score = 287, P = 3.7e-21

PIR:I49502 ankyrin - mouse, N = 3, Score = 365, P = 2.2e-27

TREMBL : HSANKY_2 product: "alt. ankyrin (variant 2.2)"; Human mRNA for ankyrin (variant 2.1), N = 2, Score = 380, P = 7.3e-31

SWISSPROT :ANK1_HUMAN ANKYRIN R (ANKYRINS 2.1 AND 2.2) (ERYTHROCYTE ANKYRIN)., N = 2, Score = 380, P = 8.2e-31

PIR:SJHUK ankyrin 1, erythrocyte splice form 1 - human, N = 2, Score = 380, P = 8.2e-31

>TREMBL : HSANKY_2 product: "alt. ankyrin (variant 2.2)"; Human mRNA for ankyrin (variant 2.1) Length = 1,719

HSPs:

Score = 380 (57.0 bits), Expect = 7.3e-31, Sum P(2) = 7.3e-31 Identities = 139/447 (31%), Positives = 207/447 (46%)

Query: 462 RGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQΞVTLLLLHYKAS 521

+G+T LH+AA+ GQ ++ LV+ GA VNA G TPL++A Q+ + V LL A+ Sbjct: 77 KGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGAN 136

Query: 522 AEVQDNNGNTPLHLACTYGHEDCVKALVYYDVES-CRL 558

V +G TPL +A GHE+ V L+ Y + RL Sbjct: 137 QNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQND 196

Query: 559 DIGNEKGDTPLHIAARWGYQGVIETLLQNGASTEIQNRLKETPLKCALNSKILSVME 615

D+ ++ G TPLHIAA + V + LL GAS + TPL A S+ +V+ Sbjct: 197 PNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIA—SRRGNVIM 254

Query: 616 AYHLSFERRQKSSEAPVQSPQRSVDSISQESSTS-SFSSMSAGSR-QEETKKDYREVEKL 673

L +R + E + + ++ S + G+ Q +TK + Sbjct: 255 V-RLLLDRGAQI-ETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHM- 311

Query: 674 LRAVADGD-LEMVRYLLEWTEEDLEDAEDTVSAADPEFCHPLCQCPKCAPAQKRLAKVPA 732

A GD L+ VR LL++ E ++D T+ P H C R+AKV Sbjct: 312 AAQGDHLDCVRLLLQYDAE-IDDI—TLDHLTP--LHVAAHC GHHRVAKVLL 358

Query: 733 S-GLGVNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQ 791

G N + +G +PLH+A ++ LLLK GA+ A PLH+A GH Sbjct: 359 DKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLP 418

Query: 792 VVKCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAV 851

+VK LL A PN ++ TPL A GH E+ LLQ+ A +NA T LH A Sbjct: 419 IVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAA 478

Query: 852 IEKHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNSKIMELLQVV 896

H +V+LLL + A+ + T + A + + +L ++ Sbjct: 479 RIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALL 523

Score = 378 (56.7 bits), Expect = 1.2e-30, Sum P(2) = 1.2e-30 Identities = 130/447 (29%), Positives = 195/447 (43%)

Query: 465 TPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKASAEV 524

TPLH AA G + ++L+ GA + A +G +P+H+A Q + LLL Y A + Sbjct: 274 TPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDD 333

Query: 525 QDNNGNTPLHLACTYGHEDCVKALVYYDVE SCR 557

+ TPLH+A GH K L+ + +C+

Sbjct: 334 ITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKT 393

Query: 558 LDIGNEKGDTPLHIAARWGYQGVIETLLQNGASTEIQNRLKETPLKCALNSKILSVM 614

+D E G TPLH+A+ G+ +++ LLQ GAS + N ETPL A + V Sb_jct: 394 GASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVA 453 Query: 615 EAYHLSFERRQKSSEAPVQΞPQRSVDSISQESSTSSFΞSMSAGSRQEETKKDYREVEKLL 674

+ Y L + + + Q+P I + +A T L

Sbjct: 454 K-YLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGH TPLH 508

Query: 675 RAVADGDLEMVRYLLEWTEEDLEDAEDTVSAADPEFCHPLCQCPKCAPAQKRLAKVPASG 734

A +G +E V LLE ++ A T P H + K A+ L + Sbjct: 509 IAAREGHVETVLALLE KEASQACMTKKGFTP--LHVAAKYGKVRVAELLLER D 559

Query: 735 LGVNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVVK 794

N ++G +PLHVA H D+++LLL G + + + PLH+A +Q +V + Sbjct: 560 AHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVAR 619

Query: 795 CLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEK 854

LL N + + G TPL A GH E+VALLL A+ N N G T LH E Sbjct: 620 SLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEG 679

Query: 855 HVFVVELLLLHGASVQVLNKRQRTAVDCAEQ--NSKIMELL 893

HV V ++L+ HG V + T + A N K+++ L Sbjct: 680 HVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFL 720

Score = 367 (55.1 bits), Expect = 1.8e-29, Sum P(2) = 1.8e-29 Identities = 131/489 (26%), Positives = 210/489 (42%)

Query: 404 HIAS—GNQKEVERLLSQEDHDKDTVQKMCHPL-CFCDDCEKLVSGRLNDPΞVVTPFSRD 460

HIAS GN V LL + + + PL C + +S L D ++ Sbjct: 244 HIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQ-AKT 302

Query: 461 DRGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKA 520

G +P+H+AA + LL+ A ++ TPLH+A G+ V +LL A Sbjct: 303 KNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGA 362

Query: 521 SAEVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGV 580

+ NG TPLH+AC H ++ L+ +D E G TPLH+A+ G+ + Sbjct: 363 KPNSRALNGFTPLHIACKKNHVRVMELLLK TGASIDAVTESGLTPLHVASFMGHLPI 419

Query: 581 IETLLQNGASTEIQNRLKETPLKCAL NSKILSVMEAYHLSFERRQKSSEAPVQSPQR 637

++ LLQ GAS + N ETPL A ++++ + + K + P+ R

Sbjct: 420 VKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAAR 479

Query: 638 SVDSISQESSTSSFSSMSAGSRQEETKKDYREVEKLLRAVADGDLEMVRYLLEWTE 693

++ + E++ + + +AG VE +L + + +T

Sbjct: 480 IGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTP 539

Query: 694 EDLEDAEDTVSAAD PEFCHPLCQ CP-KCAPAQKRLAKVPA SGLGVNVTS 741

+ V A+ HP P A L V G + +

Sbjct: 540 LHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPA 599

Query: 742 QDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVVKCLLDSNA 801

+G +PLH+AA + ++ R LL++G +A A + PLHLA Q+GH ++V LL A Sbjct: 600 WNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQA 659

Query: 802 KPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEKHVFVVEL 861

N + SG TPL GH + +L++HG ++A+ G T LH A ++ +V+ Sbjct: 660 NGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKF 719

Query: 862 LLLHGASVQVLNK 874

LL H A V K Sbjct: 720 LLQHQADVNAKTK 732

Score = 345 (51.8 bits), Expect = 4.2e-27, Sum P(2) = 4.2e-27 Identities = 146/506 (28%), Positives = 233/506 (46%)

Query: 404 HIAS--GNQKEVERLLSQEDHDKDTVQK MCHPLCFCDDCEKLVSGRLNDPSVVTPFS 458

H+AS G+ K V LL +E + T +K H +++V +N + V + Sbjct: 50 HLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQ-DEVVRELVNYGANVN—A 106

Query: 459 RDDRGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHY 518

+ +G TPL++AA ++ L+ GA N G TPL +A Q+G+++V L++Y Sbjct: 107 QSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINY 166

Query: 519 KASAEVQDNNGNTP-LHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGY 577

+V+ P LH+A ++D A V + D+ ++ G TPLHIAA + Sbjct: 167 GTKGKVR LPALHIAAR—NDDTRTAAVLLQNDP-NPDVLSKTGFTPLHIAAHYEN 218

Query: 578 QGVIETLLQNGASTEIQNRLKETPLKCAL NSKILSVMEAYHLSFERRQKSSEAPVQS 634

V + LL GAS + TPL A N ++ ++ E + K P+ Sbjct: 219 LNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHC 278

Query: 635 PQRSVDSISQESSTSSFSSMSAGSRQEETKKDYREVEKLLRAVADGD-LEMVRYLLEWTE 693

R+ E + + A +TK + A GD L+ VR LL++ Sbjct: 279 AARNGHVRISEILLDHGAPIQA KTKNGLΞPIHM AAQGDHLDCVRLLLQYDA 329 Query: 694 EDLEDAE-DTVSAAD-PEFC--HPLCQC PK CAPAQKRLAK 729

E ++D D ++ C H + + P C R+ +

Sbjct: 330 E-IDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVME 388

Query: 730 VPA-SGLGVNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQG 788

+ +G ++ ++ G +PLHVA+ G +++ LL+ GA+ N PLH+A + G Sb ct: 389 LLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAG 448

Query: 789 HFQVVKCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALH 848

H +V K LL + AK N K TPL A GH +V LLL++ A+ N + G+T LH Sbjct: 449 HTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLH 508

Query: 849 EAVIEKHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNSKIM—ELL 893

A E HV V LL AS + K+ T + A + K+ ELL Sbjct: 509 IAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELL 555

Score = 243 (36.5 bits), Expect = 1.6e-14, Sum P(2) = 1.6e-14 Identities = 64/199 (32%), Positives = 97/199 (48%)

Query: 404 HIAS—GNQKEVERLLSQEDHDKDTVQKMCHPLCFCDDCEKLVSGRLNDPSVVTPFSRDD 461

H+A+ G + E LL ++ H + PL L +L P +P S Sbjct: 541 HVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAW 600

Query: 462 RGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKAS 521

G+TPLH+AA Q + L+ G NA G TPLHLA Q+G+ + LLL +A+ Sbjct: 601 NGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQAN 660

Query: 522 AEVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGVI 581

+ + +G TPLHL GH L+ + V +D G TPLH+A+ +G ++ Sbjct: 661 GNLGNKSGLTPLHLVAQEGHVPVADVLIKHGV MVDATTRMGYTPLHVASHYGNIKLV 717

Query: 582 ETLLQNGASTEIQNRLKETPL 602

+ LLQ+ A + +L +PL Sbjct: 718 KFLLQHQADVNAKTKLGYSPL 738

Score = 242 (36.3 bits), Expect = 5.0e-29, Sum P(2) = 5.0e-29 Identities = 63/176 (35%), Positives = 92/176 (52%)

Query: 734 GLGVNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVV 793

G VN T Q+G +PLH+A+ G ++RLLL GA + D+ PLH A + GH ++ Sbjct: 229 GASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRIS 288

Query: 794 KCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIE 853

+ LLD A K +G +P+ A G H + V LLLQ+ A 1+ T LH A Sbjct: 289 EILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHC 348

Query: 854 KHVFVVELLLLHGA—SVQVLNKRQRTAVDCAEQNSKIMELLQVVPSCVASLDDVAET 909

H V ++LL GA + + LN + C + + ++MELL AS+D V E+ Sbjct: 349 GHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTG ASIDAVTES 403

Score = 242 (36.3 bits), Expect = 3.3e-14, Sum P(2) = 3.3e-14 Identities = 80/284 (28%), Positives = 129/284 (45%)

Query: 404 HIAS—GNQKEVERLLSQEDHDKDTVQKMCHPLCFCDDCEKLVΞGRLNDPSVVTPFSRDD 461

HIA+ G+ + V LL +E +K PL K+ L P + Sbjct: 508 HIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGK 567

Query: 462 RGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKAS 521

G TPLHVA ++ LL+ +G ++ ++G TPLH+A ++ V LL Y S Sbjct: 568 NGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGS 627

Query: 522 AEVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGVI 581

A + G TPLHLA GH + V L+ ++GN+ G TPLH+ A+ G+ V

Sbjct: 628 ANAESVQGVTPLHLAAQEGHAEMVALLLSKQANG NLGNKSGLTPLHLVAQEGHVPVA 684

Query: 582 ETLLQNGASTEIQNRLKETPLKCAL NSKILΞVMEAYHLSFERRQKSSEAPV-QSPQR 637

+ L+++G + R+ TPL A N K++ + + + K +P+ Q+ Q+ Sbjct: 685 DVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQ 744

Query: 638 S-VDSISQ—ESSTSSFSSMSAGSRQEETKK—DYREVEKLLRAVAD 679

D ++ ++ Ξ S G+ K Y V +L+ V D Sbjct: 745 GHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTD 791

Score = 235 (35.3 bits), Expect = 7.9e-34, Sum P(2) = 7.9e-34 Identities = 58/165 (35%), Positives = 83/165 (50%)

Query: 734 GLGVNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVV 793

G N S G +PLH+AA G A+++ LLL AN N PLHL Q+GH V Sbjct: 625 GGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVA 684 Query: 794 KCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIE 853

L+ + G TPL A G+ +LV LLQH A +NA G + LH+A + Sbjct: 685 DVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQ 744

Query: 854 KHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNS—KIMELLQVV 896

H +V LLL +GAS ++ T + A++ + ++L+VV Sbjct: 745 GHTDIVTLLLKNGASPNEVΞSDGTTPLAIAKRLGYISVTDVLKVV 789

Score = 233 (35.0 bits), Expect = 7.9e-34, Sum P(2) = 7.9e-34 Identities = 67/202 (33%), Positives = 100/202 (49%)

Query: 404 HIAS-GNQKEVERLLSQEDHDKDTVQKMCH—PLCFCDDC-EKLVSGRLNDPSVVTPFSR 459

H+A+ G+ + RLL Q D + D + + H PL C V+ L D P SR Sbjct: 310 HMAAQGDHLDCVRLLLQYDAEIDDIT-LDHLTPLHVAAHCGHHRVAKVLLDKGA-KPNSR 367

Query: 460 DDRGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYK 519

G TPLH+A +++LL+ GA ++A G TPLH+A G+ + LL Sbjct: 368 ALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRG 427

Query: 520 ASAEVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQG 579

AS V + TPLH+A GH + K L+ +++ + TPLH AAR G+ Sbjct: 428 ASPNVSNVKVETPLHMAARAGHTEVAKYLLQ NKAKVNAKAKDDQTPLHCAARIGHTN 484

Query: 580 VIETLLQNGASTEIQNRLKETPLKCA 605

+++ LL+N A+ + TPL A Sbjct: 485 MVKLLLENNANPNLATTAGHTPLHIA 510

Score = 226 (33.9 bits), Expect = 7.0e-33, Sum P(2) = 7.0e-33 Identities = 53/153 (34%), Positives = 83/153 (54%)

Query: 743 DGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVVKCLLDSNAK 802

+G +PLH+AA + ++ R LL++G +A A + PLHLA Q+GH ++V LL A Sbjct: 601 NGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQAN 660

Query: 803 PNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEKHVFVVELL 862

N + SG TPL GH + +L++HG ++A+ G T LH A ++ +V+ L Sbjct: 661 GNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFL 720

Query: 863 LLHGASVQVLNKRQRTAVDCAEQ--NSKIMELL 893

L H A V K + + A Q ++ I+ LL Sbjct: 721 LQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLL 753

Score = 198 (29.7 bits), Expect = 2.5e-ll, Sum P(2) = 2.5e-ll Identities = 51/157 (32%), Positives = 82/157 (52%)

Query: 737 VNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVVKCL 796

+ T++ G++ LH+AAL G+ +++R L+ +GAN A++ PL++A Q+ H +VVK L Sbjct: 71 LETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFL 130

Query: 797 LDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEKHV 856

L++ A N G TPL A GH +VA L+ +G ALH A

Sbjct: 131 LENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTK GKVRLPALHIAARNDDT 186

Query: 857 FVVELLLLHGASVQVLNKRQRTAVDCAE—QNΞKIMELL 893

+LL + + VL+K T + A +N + +LL Sbjct: 187 RTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLL 225

Score = 186 (27.9 bits), Expect = 6.6e-29, Sum P(2) = 6.6e-29 Identities = 55/143 (38%), Positives = 68/143 (47%)

Query: 463 GHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKASA 522

GHTPLH+AA G + L+ K A G TPLH+A + G V LLL A Sb_jct: 503 GHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHP 562

Query: 523 EVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGVIE 582

NG TPLH+A + + D VK L+ S N G TPLHIAA+ V Sbjct: 563 NAAGKNGLTPLHVAVHHNNLDIVKLLLPRG-GSPHSPAWN—GYTPLHIAAKQNQVEVAR 619

Query: 583 TLLQNGASTEIQNRLKETPLKCA 605

+LLQ G S ++ TPL A Sbjct: 620 SLLQYGGSANAESVQGVTPLHLA 642

Score = 182 (27.3 bits), Expect = 2.9e-28, Sum P(2) = 2.9e-28 Identities = 54/185 (29%), Positives = 89/185 (48%)

Query: 738 NVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVVKCLL 797

N+ ++ G +PLH+ A G + +L+KHG A PLH+A G+ ++VK LL Sbjct: 662 NLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLL 721

Query: 798 DΞNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEKHVF 857 A N K G +PL A GH ++V LLL++GAS N ++ G T L A ++ Sbjct: 722 QHQADVNAKTKLGYSPLH_QAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYIS 781

Query: 858 VVELLLLHGASVQVLNKRQRTAVDCAEQNSKIMELLQVVPSCVASLDDVAETDRKEYVTV 917

V ++L + V ++ V + S P V + DV+E + +E ++ Sbjct: 782 VTDVLKV VTDETSFVLVSDKHRMS FPETVDEILDVSEDEGEELISF 827

Query: 918 KIRKK 922

K ++ Sbjct: 828 KAERR 832

Score = 180 (27.0 bits), Expect = 5.0e-29, Sum P(2) = 5.0e-29 Identities = 41/121 (33%), Positives = 67/121 (55%)

Query: 486 GAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKASAEVQDNNGNTPLHLACTYGHEDCV 545

G +N + +G LHLA ++G+ + + LLH + E GNT LH+A G ++ V Sbjct: 35 GVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVV 94

Query: 546 KALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGVIETLLQNGASTEIQNRLKETPLKCA 605

+ LV Y ++ ++KG TPL++AA+ + V++ LL+NGA+ + TPL A Sbjct: 95 RELVNY GANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVA 151

Query: 606 L 606

L Sbjct: 152 L 152

Score = 166 (24.9 bits), Expect = 3.4e-06, Sum P(2) = 3.4e-06 Identities = 89/318 (27%), Positives = 140/318 (44%)

Query: 448 LNDPSVVTPFSRDDRGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKG 507

L + + V ++DD+ TPLH AA G +++ LL+ A N G TPLH+A ++G Sbjct: 457 LQNKAKVNAKAKDDQ—TPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREG 514

Query: 508 YQSVTLLLLHYKASAEVQDNNGNTPLHLACTYGHEDCVKALVYYD 552

+ L LL +AS G TPLH+A YG + L+ D Sbjct: 515 HVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLH 574

Query: 553 —VESCRLDI GNE KGDTPLHIAARWGYQGVIETLLQNGASTEIQNRL 597

V LDI G+ G TPLHIAA+ V +LLQ G S ++ Sbjct: 575 VAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQ 634

Query: 598 KETPLKCALNSKILSVMEAYHLSFERRQKSSEAPVQΞPQRSVDSISQESSTSSFSSM-SA 656

TPL A M A LS +Q + +S + ++QE + Sbjct: 635 GVTPLHLAAQEGHAE-MVALLLS KQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKH 690

Query: 657 GSRQEETKKDYREVEKLLRAVADGDLEMVRYLLEWTEEDLEDAEDTVSAADPEFCHPLCQ 716

G + T + L A G++++V++LL+ + D+ +A+ + + PL Q Sbjct: 691 GVMVDATTR— GYTPLHVASHYGNIKLVKFLLQH-QADV-NAKTKLGYΞ PLHQ 740

Query: 717 CPKCAPAQKRLAKVPASGLGVNVTSQDGSSPLHVA 751

+ + + +G N S DG++PL +A Sbjct: 741 AAQQGHTDI-VTLLLKNGASPNEVSSDGTTPLAIA 774

Score = 162 (24.3 bits), Expect = 1.8e-07, Sum P(2) = 1.8e-07 Identities = 48/149 (32%), Positives = 71/149 (47%)

Query: 737 VNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVVKCL 796

V D ++ AA G D L++G + N + LHLA ++GH ++V L Sbjct: 5 VGFREADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVEL 64

Query: 797 LDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEKHV 856

L GNT L A G E+V L+ +GA++NA + KG T L+ A E H+

Sbjct: 65 LHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHL 124

Query: 857 FVVELLLLHGASVQVLNKRQRTAVDCAEQ 885

VV+ LL +GA+ V + T + A Q Sbjct: 125 EVVKFLLENGANQNVATEDGFTPLAVALQ 153

Score = 158 (23.7 bits), Expect = 5.7e-26, Sum P(2) = 5.7e-26 Identities = 38/135 (28%), Positives = 65/135 (48%)

Query: 460 DDRGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYK 519

+ G LH+A+ G ++ L+ K ++ T G T LH+A G V L++Y Sbjct: 42 NQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYG 101

Query: 520 ASAEVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQG 579

A+ Q G TPL++A H + VK L+ ++ E G TPL +A + G++

Sbjct: 102 ANVNAQSQKGFTPLYMAAQENHLEVVKFLLE NGANQNVATEDGFTPLAVALQQGHEN 158

Query: 580 VIETLLQNGASTEIQ 594

V+ L+ G +++ Sbjct: 159 VVAHLINYGTKGKVR 173 Score = 115 (17.3 bits), Expect = 1.8e-21, Sum P(2) = 1.8e-21 Identities = 37/119 (31%), Positives = 58/119 (48%)

Query: 497 ATPLHLACQKGYQSVTLLLLHYKASAEVQ—DNNGNTPLHLACTYGHEDCVKALVYYDVE 554

AT A + G ++ L H + ++ + NG LHLA GH V L++ ++ Sbjct: 13 ATΞFLRAARΞG—NLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEII 70 Query: 555 SCRLDIGNEKGDTPLHIAARWGYQGVIETLLQNGASTEIQNRLKETPLKCALNΞKILSVM 614 L+ +KG+T LHIAA G V+ L+ GA+ Q++ TPL A L V+ Sbjct: 71 LETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVV 127 Query: 615 E 615

+ Sbjct: 128 K 128

Score = 106 (15.9 bits), Expect = 1.8e-01, Sum P(2) = 1.6e-01 Identities = 34/121 (28%), Positives = 54/121 (44%)

Query: 769 NAGARNADQAVPLHLACQQGHFQVVKCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVAL 828

+ G R AD A A + G+ L + N + +G L A GH ++V Sbjct: 4 SVGFREADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVE 63

Query: 829 LLQHGASINASNNKGNTALHEAVIEKHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNSK 888

LL + + KGNTALH A + VV L+ +GA+V +++ T + A Q + Sbjct: 64 LLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENH 123

Query: 889 I 889

+ Sbjct: 124 L 124

Score = 40 (6.0 bits), Expect = 1.6e-14, Sum P(2) 1.6e-14 Identities = 11/56 (19%), Positives = 23/56 (41%)

Query: 622 ERRQKSSEAPVQSPQRSVDSISQESSTSSFSSMSAGSRQEETKKDYREVEKLLRAV 677

+RRQ+ E VQ + + + Q + + Q ++ +K++R V

Sbjct: 1614 DRRQQGQEEQVQEAKNTFTQVVQGNEFQNIPGEQVTEEQFTDEQGNIVTKKIIRKV 1669

Score = 38 (5.7 bits), Expect = 2.6e-14, Sum P(2) = 2.6e-14 Identities = 6/12 (50%), Positives = 10/12 (83%)

Query: 806 KDLSGNTPLIYA 817

+D++G T L+YA Sbjct: 1186 EDITGTTKLVYA 1197

Pedant information for DKFZphtes3_1817, frame 2

Report for DKFZphtes3_1817.2

[LENGTH] 1050 [MW] 117013.72 [pl] 6.47 [HOMOL] TREMBL : DMANKY_1 product: "ankyrin"; Drosophila melanogaster ankyrin mRNA, complete eds . 2e-45 [FUNCAT] 08.19 cellular import [S. cerevisiae, YOR034c] 5e-13 [FUNCAT] 10.05.99 other pheromone response activities [S. cerevisiae, YDR264c] 3e-12 [FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [ S. cerevisiae, YDR264c] 3e-12 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YIL112w] 2e-ll [FUNCAT] 06.13.01 cytoplasmic degradation [S. cerevisiae, YGR232w] 8e-10 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YIR033w] 2e-08 [FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YIR033w] 2e-08 [FUNCAT] 01.04.04 regulation of phosphate utilization [S. cerevisiae, YGR233c] 3e-08 [FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YML097c] 5e-05 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YML097c] 5e-05 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YML097C] 5e-05 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YML097c] 5e-05 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YERlllc] 3e-04 [ FUNCA ] 04.05.01.04 transcriptional control [S. cerevisiae, YERlllc] 3e-04 [BLOCKS] BL00901A Cysteine synthase/cystathionme beta-synthase P-phosphate att [SCOP] dlawcb_ 1.91.3.1.2 GA binding protein (GABP) alpha GA bindini 4e-12 [EC] 3.1.3.53 Myosm-light-chain-phosphatase le-12 [PIRKW] phosphotransferase le-19 [PIRKW] nucleus le-13 [PIRKW] potassium channel 5e-15

[PIRKW] early protein 2e-13

[PIRKW] tumor suppressor le-09

[PIRKW] duplication le-14

[PIRKW] tandem repeat le-19

[PIRKW] heterodimer le-14

[PIRKW] potassium transport 5e-15

[PIRKW] cell cycle control le-10

[PIRKW] serine/threon e-specific protein kmase le-19

[PIRKW] transmembrane protein 5e-15

[PIRKW] transport protein 5e-15

[PIRKW] DNA binding 2e-ll

[PIRKW] oncogene le-08

[PIRKW] ATP le-19

[PIRKW] protein kinase inhibitor le-09

[PIRKW] voltage-gated ion channel 5e-15

[PIRKW] phosphoprotein 4e-38

[PIRKW] apoptosis le-19

[PIRKW] liver 4e-09

[PIRKW] mtegπn binding 3e-16

[PIRKW] differentiation 2e-12

[PIRKW] transforming protein le-08

[PIRKW] alternative splicing le-40

[PIRKW] coiled coil le-14

[PIRKW] peripheral membrane protein 2e-38

[PIRKW] transcription factor 4e-16

[PIRKW] transcription regulation 2e-16

[PIRKW] nucleotide binding 5e-15

[PIRKW] phosphoric monoester hydrolase le-12

[PIRKW] cytoskeleton 8e-39

[PIRKW] calmodulin binding le-19

[PIRKW] smooth muscle le-12

[SUPFAM] ankyrin le-40

[SUPFAM] death-associated protein kinase le-19

[SUPFAM] ankyrin repeat homology le-40

[SUPFAM] protein kinase homology le-19

[SUPFAM] vaccinia virus 27.4K Hindlll-C protein homology 3e-07

[SUPFAM] mt-3 transforming protein le-08

[SUPFAM] unassigned ankyrin repeat proteins 2e-38

[SUPFAM] notch protein 2e-12

[SUPFAM] fowlpox virus BamHI-ORF7 protein 2e-13

[SUPFAM] rel homology 2e-ll

[SUPFAM] EGF homology 2e-12

[PROSITE] ATP_GTP_A 1

[PFAM] Ank repeat

[KW] Irregular

[KW] 3D

[KW] LOW_COMPLEXITY 3.05 %

SEQ MALYDEDLLKNPFYLALQKCRPDLCSKVAQIHGIVLVPCKGSLSSSIQSTCQFESYILIP SEG lawcB

SEQ VEEHFQTLNGKDVFIQGNRIKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLEKR SEG lawcB

SEQ ESSEEPLAPSDPFSLKTIEDVREFLGRHSERFDRNIASFHRTFRECERKSLRHHIDSANA SEG lawcB

SEQ LYTKCLQQLLRDSHLKMLAKQEAQMNLMKQAVEIYVHHEIYNLIFKYVGTMEAΞEDAAFN SEG lawcB

SEQ KITRSLQDLQQKDIGVKPEFSFNIPRAKRELAQLNKCTSPQQKLVCLRKVVQLITQSPSQ SEG lawcB

SEQ RVNLETMCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSLAKDELGYCLTSFEAAIE SEG xxxxxxxxxx lawcB

SEQ YIRQGSLSAKPPESEGFGDRLFLKQRMSLLSQMTSSPTDCLFKHIASGNQKEVERLLSQE SEG lawcB

SEQ DHDKDTVQKMCHPLCFCDDCEKLVSGRLNDPSVVTPFSRDDRGHTPLHVAAVCGQASLID SEG lawcB SEQ LLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKASAEVQDNNGNTPLHLACTYG SEG lawcB

SEQ HEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGVIETLLQNGASTEIQNRLKET SEG lawcB

SEQ PLKCALNSKILSVMEAYHLSFERRQKSSEAPVQSPQRSVDSISQESSTSSFSSMSAGSRQ SEG xxxxxxxxxxxxxxxxxxxxxx . lawcB

SEQ EETKKDYREVEKLLRAVADGDLEMVRYLLEWTEEDLEDAEDTVSAADPEFCHPLCQCPKC SEG lawcB

SEQ APAQKRLAKVPASGLGVNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVP SEG lawcB CHHHHHHHHHHHCCHHHHHHHHHHCCCC-CCTTTTCCH

SEQ LHLACQQGHFQVVKCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASN SEG lawcB HHHHHHHCCHHHHHHHHHCCCTTTTCTTTTCCHHHHHHHHTTHHHHHHHHHCCCTTTTEE

SEQ NKGNTALHEAVIEKHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNSKIMELLQVVPΞCV SEG lawcB TTTEEHHHHHHHHCCHHHHHHHHHHCCTTTTCBTTTBCHHHHHHHHCCHHHHHC

SEQ ASLDDVAETDRKEYVTVKIRKKWNSKLYDLPDEPFTRQFYFVHSAGQFKGKTSREIMARD

SEG lawcB

SEQ RSVPNLTEGSLHEPGRQΞVTLRQNNLPAQSGSHAAEKGNSDWPERPGLTQTGPGHRRMLR SEG lawcB

SEQ RHTVEDAVVSQGPEAAGPLSTPQEVSASRS SEG lawcB

Prosite for DKFZphtes3_1817.2

PΞ00017 945->953 ATP GTP A PDOC00017

Pfam for DKFZphtes3_1817.2

HMM_NAME Ank repeat

HMM *GyTPLHIAARyNNvEMVrlLLQHGADIN* G+TPLH+AA ++ ++++LL+++GA +N

Query 463 GHTPLHVAAVCGQASLIDLLVSKGAMVN 490

32.12 (bits) f: 496 t: 523 Target: dkfzphtes3_1817.2 similarity to ankyrins

Alignment to HMM consensus : Query *GyTPLHIAARyNNvEMVrlLLQHGADIN*

G TPLH+A++ + ++ LLL + A+ dkfzphtes3 496 GATPLHLACQKGYQSVTLLLLHYKASAE 523

Query f: 529 t: 556 Target: dkfzphtes3_1817.2 similarity to ankyrins

Alignment to HMM consensus: HMM *GyTPLHIAARyNNvEMVrlLLQHGADIN*

G+TPLH+A+ Y+++++V+ L+ + Query 529 GNTPLHLACTYGHEDCVKALVYYDVESC 556

42.65 (bits) f: 565 t: 592 Target: dkfzphtes3_1817.2 similarity to ankyrins

Alignment to HMM consensus: Query *GyTPLHIAARyNNvEMVrlLLQHGADIN*

G+TPLHIAAR + +++ LLQ+GA+ dkfzphtes3 565 GDTPLHIAARWGYQGVIETLLQNGAΞTE 592

Query f: 744 t: 771 Target: dkfzphtes3_1817.2 similarity to ankyrins

Alignment to HMM consensus: HMM *GyTPLHIAARyNNvEMVrlLLQHGADIN*

G +PLH+AA +++ +++RLLL+HGA+ Query 744 GSSPLHVAALHGRADLIRLLLKHGANAG 771 36.38 (bits) f: 777 t: 804 Target: dkfzphtes3_1817.2 similarity to ankyrins

Alignment to HMM consensus: Query *GyTPLHIAARyNNvEMVrlLLQHGADIN*

PLH+A+++++ ++V+ LL+ +A +N dkfzphtes3 777 QAVPLHLACQQGHFQVVKCLLDSNAKPN 804

Query f: 810 t: 837 Target: dkfzphtes3_1817.2 similarity to ankyrins

Alignment to HMM consensus : HMM *GyTPLHIAARyNNvEMVrlLLQHGADIN*

G+TPL++A+ ++ E+V LLLQHGA+IN Query 810 GNTPLIYACSGGHHELVALLLQHGASIN 837

44.62 (bits) f: 843 t: 870 Target: dkfzphtes3_1817.2 similarity to ankyrins

Alignment to HMM consensus: Query *GyTPLHIAARyNNvEMVrlLLQHGADIN*

G+T+LH A+++ +V +V+LLL HGA++ dkfzphtes3 843 GNTALHEAVIEKHVFVVELLLLHGASVQ 870

DKFZphtes3_19fl9

group: testes derived

DKFZphtes3_19f19 encodes a novel 254 amino acid protein with weak similarity to S. cerevisiae protein YFL046w.

The protein contains a RGD cell attachment site.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . similarity to YFL046w localisation: 3 STS match perfect but HS1292427 matches to chromosome 4

Sequenced by MediGenomix

Locus: /map="405.0/.3 cR from top of Chrll linkage group"

Insert length: 1395 bp

Poly A stretch at pos. 1367, no polyadenylation signal found

1 GGGACCACGG TGGCGCCTGC GCTGGGAGGT GAGCTTGTGA CAGAGCGAAA 51 ACTACAATTC CCAGCATTCC TGTGGTGCCA GAACTACCTT GCCCGAAAGC

101 CTGTGCGAGA TTTACCCCGT CTTCCGCCTC CCTCCCACCG GAAAACTCTG

151 AGGACATGAA TAGTCGCCAG GCTTGGCGGC TCTTTCTCTC CCAAGGCAGA

201 GGAGATCGTT GGGTTTCAAG GCCCCGCGGG CATTTCTCGC CGGCCCTGCG

251 GAGAGAGTTC TTCACTACCA CAACCAAGGA GGGATATGAT AGGCGGCCAG

301 TGGATATAAC TCCTTTAGAA CAAAGGAAAT TAACTTTTGA TACCCATGCA

351 TTGGTTCAGG ACTTGGAAAC TCATGGATTT GACAAAACAC AAGCAGAAAC

401 AATTGTATCA GCGTTAACTG CTTTATCAAA TGTCAGCCTG GATACTATCT

451 ATAAAGAGAT GGTCACTCAA GCTCAACAGG AAATAACAGT ACAACAGCTA

501 ATGGCTCATT TGGATGCTAT CAGGAAAGAC ATGGTCATCC TAGAGAAAAG

551 TGAATTTGCA AATCTGAGAG CAGAGAATGA GAAAATGAAA ATTGAATTAG

601 ACCAAGTTAA GCAACAACTA ATGCATGAAA CCAGTCGAAT CAGAGCAGAT

651 AATAAACTGG ATATCAACTT AGAAAGGAGC AGAGTAACAG ATATGTTTAC

701 AGATCAAGAA AAGCAACTTA TGGAAACAAC TACAGAATTT ACAAAAAAGG

751 ATACTCAAAC CAAAAGTATT ATTTCAGAGA CCAGTAATAA AATTGACGCT

801 GAAATTGCTT CCTTAAAAAC ACTGATGGAA TCTAACAAAC TTGAGACAAT

851 TCGTTATCTT GCAGCTTCGG TGTTTACTTG CCTGGCAATA GCATTGGGAT

901 TTTATAGATT CTGGAAGTAG TATTAATGCT CATCCTGCTG TGGCTGTTGG

951 CTTCTTAGAA CACCAAACCG GGAGAGATTT ACTTTGAACA TTGTCAGTTG 1001 CAGCAAAAAT TTACTACACA AGATTATTCG AAGTGTATAC GGACTAAAAG 1051 AGGAAGTGTT TTAGAATGAG AAGAGATACT GTGTCTTTAT TGTGTGTGTG 1101 TGAGTGCAGG TGTGTGTCTT TATTATATTG AAAAGCTGTC ACTCAGACCT 1151 GGTTTGAGAT AGAAGAGCAT TTTGTCCTTT TGATAGTTAA TAGAAATTGA 1201 ACCAGAGTTT TCTTATGTTT GCTTGAACAG TTGTGTAAAT CATACAGGAT 1251 TTTGTGGGTA TTGGTTGAAT ATTTGTAAAC CATTCCCTAG CCTACATATT 1301 TATTACTGAA TTAACTTTCC TGATAACCAT TGCATAATTA CATTTTTCTA 1351 TAAAATGAAA GATTATTACA ACAAAAAAAA AAAAAAAAAA AAAAA

BLAST Results

Entry HS419346 from database EMBL: human STS WI-13569. Score = 2154, P = 8.6e-91, identities = 446/459

Entry HS1292427 from database EMBL: human STS SHGC-50338. Score = 1737, P = 7.2e-72, identities = 359/369

Entry HS253344 from database EMBL: human STS WI-13893. Score = 1578, P = 1.0e-64, identities = 358/397

Medline entries

No Medline entry Peptide information for frame 3

ORF from 156 bp to 917 bp; peptide length: 254 Category: similarity to unknown protein Classification: no clue Prosite motifs: RGD (15-18)

1 MNSRQAWRLF LSQGRGDRWV SRPRGHFSPA LRREFFTTTT KEGYDRRPVD 51 ITPLEQRKLT FDTHALVQDL ETHGFDKTQA ETIVSALTAL SNVSLDTIYK 101 EMVTQAQQEI TVQQLMAHLD AIRKDMVILE KSEFANLRAE NEKMKIELDQ 151 VKQQLMHETΞ RIRADNKLDI NLERSRVTDM FTDQEKQLME TTTEFTKKDT 201 QTKSIISETS NKIDAEIASL KTLMESNKLE TIRYLAASVF TCLAIALGFY 251 RFWK

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_19f19, frame 3

SWISSPROT :YAN8_SCHPO HYPOTHETICAL 24.6 KD PROTEIN C3H1.08 IN CHROMOSOME I., N = 1, Score = 144, P = 8.4e-09

PIR:S56209 probable membrane protein YFL046w - yeast (Saccharomyces cerevisiae), N = 1, Score = 138, P = 5.4e-08

>SWISSPROT:YAN8_ΞCHPO HYPOTHETICAL 24.6 KD PROTEIN C3H1.08 IN CHROMOSOME I. Length = 211

HSPs:

Score = 144 (21.6 bits), Expect = 8.4e-09, P = 8.4e-09 Identities = 34/121 (28%), Positives = 67/121 (55%)

Query: 70 LETHGFDKTQAETIVSALTALSNVSLDTIYKEMVTQAQQE-ITVQQLMAHLDAIRKDMVI 128

LE G+ AETI + + ++ +L + K + +A+QE ++ QQ L IRK + Sbjct: 46 LEQAGYSVKNAETITNLMRTITGEALTELEKNIGFKAKQESVSFQQKRTFLQ-IRKYLET 104 Query: 129 LEKSEFANLRAENEKMKIELDQVKQQLMHETSRIRADNKLDINLERSRVTDMFTDQEKQL 188

+E++EF +R ++K+ E+++ K L + ++ +L++NLE+ R+ D T + + Sbjct: 105 IEENEFDKVRKSSDKLINEIEKTKSSLREDVKTALSEVRLNLNLEKGRMKDAATSRNTNI 164 Query: 189 ME 190

E Sbjct: 165 HE 166

Pedant information for DKFZphtes3_19f19, frame 3

Report for DKFZphtes3_19f19.3

[LENGTH] 254

[MW] 29505.73

[pi] 6.99

[HOMOL] PIR:S56209 probable membrane protein YFL046w - yeast (Saccharomyces cerevisiae)

2e-10

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YFL046w] 8e-12

[PROSITE] RGD 1

[KW] TRANSMEMBRANE 1

[KW] LOW_COMPLEXITY 5.12 %

[KW] COILED COIL 11.02 %

SEQ MNSRQAWRLFLSQGRGDRWVΞRPRGHFSPALRREFFTTTTKEGYDRRPVDITPLEQRKLT

SEG

PRD ccchhhhhhhhhccccceeeeccccccchhhhhhheeeeccccccccccccchhhhhhcc

COILS

MEM

SEQ FDTHALVQDLETHGFDKTQAETIVSALTALSNVSLDTIYKEMVTQAQQEITVQQLMAHLD

SEG

PRD chhhhhhhhhhhcccccchhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS MEM

SEQ AIRKDMVILEKSEFANLRAENEKMKIELDQVKQQLMHETSRIRADNKLDINLERSRVTDM

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCC

MEM

SEQ FTDQEKQLMETTTEFTKKDTQTKSIISETSNKIDAEIASLKTLMESNKLETIRYLAASVF

SEG xxxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhcccccccceeeeehhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS

MEM MMMMMMM

SEQ TCLAIALGFYRFWK

SEG

PRD hhhhhhhhhhhccc

COILS

MEM MMMMMMMMMM....

Prosite for DKFZphtes3_19f19.3 PS00016 15->18 RGD PDOC00016

(No Pfam data available for DKFZphtes3_19f19.3)

DKFZphtes3_19jl7

group: testes derived

DKFZphtes3_19jl7 encodes a novel 436 amino acid protein with partial similarity to C. elegans Y40B1A.2 protein.

The novel protein contains two Prosite WW/rsp5/WWP domain signatures.

The WW domain (or rsp5 or WWP domain) has been originally discovered as a short conserved region in a number of unrelated proteins, such as dystrophin, utrophin, vertebrate YAP protein, mouse NEDD-4 and yeast RSP5.

The domain is repeated up to 4 times in some proteins. It has been shown to bind proteins with particular prolme-motifs, [AP] -P-P- [AP] -Y, and thus resembles somewhat SH3 domains. It appears to contain beta-strands grouped around four conserved aromatic positions; generally

Trp. The name WW or WWP derives from the presence of these Trp as well as that of a conserved

Pro. It is frequently associated with other domains typical for proteins in signal transduction processes.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . similarity to C. elegans Y40B1A.2 there are two long ORFs in this cDNA according to EST: HS12146/HΞ75086/AA923755/MMAA17335 remaining intron at Bp 1506-1733

Sequenced by MediGenomix

Locus : unknown

Insert length: 2762 bp

Poly A stretch at pos. 2740, no polyadenylation signal found

1 ATTCTCAGCC AAATTTTTTT ATTTTTTGCA GAATCAGTGT GCAAGGTGGT

51 TTATAAGATA ATGGAGTGGT TTTTTTTTGT GTTTAGTGTG ATTTGTTATC 101 AGGAGTCTTA TTGTAACGCT TAAGCATTAG GTTTTTTGTC TGAGAAACTT 151 TAAAGAGTAA AGCAGAATTG AAAGTGGAAA TTTTAATTTT GTAAGTTCAT 201 AAAATTTAAT GATAATACAC CAAAGTTTAT GTTTAAATTA GGGAGTTTAA 251 GGTTTCAATT CTTTCTCTTT TTTTTTGGGG GGGTGATGTT TTACAGGCAC 301 TTAAGTATTC ATCGAAGAGT CACCCCAGTA GCGGTGATCA CAGACATGAA 351 AAGATGCGAG ACGCCGGAGA TCCTTCACCA CCAAATAAAA TGTTGCGGAG 401 ATCTGATAGT CCTGAAAACA AATACAGTGA CAGCACAGGT CACAGTAAGG 451 CCAAAAATGT GCATACTCAC AGAGTTAGAG AGAGGGATGG TGGGACCAGT 501 TACTCTCCAC AAGAAAATTC ACACAACCAC AGTGCTCTTC ATAGTTCAAA 551 TTCACATTCT TCTAATCCAA GCAATAACCC AAGCAAAACT TCAGATGCAC 601 CTTATGATTC TGCAGATGAC TGGTCTGAGC ATATTAGCTC TTCTGGGAAA 651 AAGTACTACT ACAATTGTCG AACAGAAGTT TCACAATGGG AAAAACCAAA 701 AGAGTGGCTT GAAAGAGAAC AGAGACAAAA AGAAGCAAAC AAGATGGCAG 751 TCAACAGCTT CCCAAAAGAT AGGGATTACA GAAGAGAGGT GATGCAAGCA 801 ACAGCCACTA GTGGGTTTGC CAGTGGAATG GAAGACAAGC ATTCCAGTGA 851 TGCCAGTAGT TTGCTCCCAC AGAATATTTT GTCTCAAACA AGCAGACACA 901 ATGACAGAGA CTACAGACTG CCAAGAGCAG AGACTCACAG TAGTTCTACG 951 CCAGTACAGC ACCCCATCAA ACCAGTGGTT CATCCAACTG CTACCCCAAG 1001 CACTGTTCCT TCTAGTCCAT TTACGCTACA GTCTGATCAC CAGCCAAAGA 1051 AATCATTTGA TGCTAATGGA GCATCTACTT TATCAAAACT GCCTACACCC 1101 ACATCTTCTG TCCCTGCACA GAAAACAGAA AGAAAAGAAT CTACATCAGG 1151 AGACAAACCC GTATCACATT CTTGCACAAC TCCTTCCACG TCTTCTGCCT 1201 CTGGACTGAA CCCCACATCT GCACCTCCAA CATCTGCTTC AGCGGTCCCT 1251 GTTTCTCCTG TTCCACAGTC GCCAATACCT CCCTTACTTC AGGACCCAAA 1301 TCTTCTTAGA CAATTGCTTC CTGCTTTGCA AGCCACGCTG CAGCTTAATA 1351 ATTCTAATGT GGACATATCT AAAATAAATG AAGTTCTTAC AGCAGCTGTG 1401 ACACAAGCCT CACTGCAGTC TATAATTCAT AAGTTTCTTA CTGCTGGACC 1451 ATCTGCTTTC AACATAACGT CTCTGATTTC TCAAGCTGCT CAGCTCTCTA 1501 CACAAGATAT CCCTCTTCAT GAAGGTATCC AAATGGAGAG AGATACACAT 1551 AGGAGCAAAT GGGAAGTGAA AGGGTCACTT TGTCAGAAAG CTGATAAACA 1601 GCAGGAATGC CTTGTCTGGA ATGGAAGTAT AATGGTGCAA AGACTCTTGC 1651 AACCCTCTGG CTAGCCTCAT GAGCAGGAGA CTGCGTGGGA TACCTGGGCC 1701 TAAATGTAGA ATAAGAAAGA AGAAATAAGG ATGCCCAGCC ATCTAATCAG 1751 TCTCCGATGT CTTTAACATC TGATGCGTCA TCCCCAAGAT CATATGTTTC 1801 TCCAAGAATA AGCACACCTC AAACTAACAC AGTCCCTATC AAACCTTTGA 1851 TCAGTACTCC TCCTGTTTCA TCACAGCCAA AGGTTAGTAC TCCAGTAGTT 1901 AAGCAAGGAC CAGTGTCACA GTCAGCCACA CAGCAGCCTG TAACTGCTGA 1951 CAAGCAGCAA GGTCATGAAC CTGTCTCTCC TCGAAGTCTT CAGCGCTCAA 2001 GCCAGAGAAG TCCATCACCT GGTCCCAATC ATACTTCTAA TAGTAGTAAT 2051 GCATCAAATG CAACAGTTGT ACCACAGAAT TCTTCTGCCC GATCCACGTG 2101 TTCATTAACG CCTGCACTAG CAGCACACTT CAGTGAAAAT CTCATAAAAC 2151 ACGTTCAAGG ATGGCCTGCA GATCATGCAG AGAAGCAGGC ATCAAGATTA 2201 CGCGAAGAAG CGCATAACAT GGGAACTATT CACATGTCCG AAATTTGTAC 2251 TGAATTAAAA AATTTAAGAT CTTTAGTCCG AGTATGTGAA ATTCAAGCAA 2301 CTTTGCGAGA GCAAAGGATA CTATTTTTGA GACAACAAAT TAAGGAACTT 2351 GAAAAGCTAA AAAATCAGAA TTCCTTCATG GTGTGAAGAT GTGAATAATT 2401 GCACATGGTT TTGAGAACAG GAACTGTAAA TCTGTTGCCC AATCTTAACA 2451 TTTTTGAGCT GCATTTAAGT AGACTTTGGA CCGTTAAGCT GGGCAAAGGA 2501 AATGACAAGG GGACGGGGTC TGTGAGAGTC AATTCAGGGG AAAGATACAA 2551 GATTGATTTG TAAAACCCTT GAAATGTAGA TTTCTTGTAG ATGTATCCTT 2601 CACGTTGTAA ATATGTTTTG TAGAGTGAAG CCATGGGAAG CCATGTGTAA 2651 CAGAGCTTAG ACATCCAAAA CTAATCAATG CTGAGGTGGC TAAATACCTA 2701 GCCTTTTACA TGTAAACCTG TCTGCAAAAT TAGCTTTTTT AAAAAAAAAA 2751 AAAAAAAAAA AA

BLAST Results

Entry AC005876 from database EMBLNEW:

Homo sapiens chromosome 10 clone CIT987ΞK-1188I5 map lOpll .2-10pl2.1, complete sequence.

Score = 2130, P = O.Oe+00, identities = 426/426 12 exons matching Bp 492-2740

Medline entries

No Medlme entry

Peptide information for frame 2

ORF from 1757 bp to 2383 bp; peptide length: 209 Category: questionable ORF Classification: no clue

1 MSLTSDASSP RSYVSPRIST PQTNTVPIKP LISTPPVSSQ PKVSTPVVKQ

51 GPVSQSATQQ PVTADKQQGH EPVSPRSLQR SSQRSPSPGP NHTSNSSNAS

101 NATVVPQNSS ARSTCSLTPA LAAHFSENLI KHVQGWPADH AEKQASRLRE

151 EAHNMGTIHM SEICTELKNL RSLVRVCEIQ ATLREQRILF LRQQIKELEK

201 LKNQNSFMV

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_19j 17, frame 2 No Alert BLASTP hits found

Peptide information for frame 3

ORF from 354 bp to 1661 bp; peptide length: 436 Category: similarity to unknown protein Classification: unclassified Prosite motifs: WW_DOMAIN_l (90-116) WW DOMAIN 1 (90-116)

1 MRDAGDPSPP NKMLRRSDSP ENKYSDSTGH SKAKNVHTHR VRERDGGTSY

51 SPQENSHNHS ALHSSNSHSS NPSNNPSKTS DAPYDSADDW SEHISSSGKK

101 YYYNCRTEVS QWEKPKEWLE REQRQKEANK MAVNSFPKDR DYRREVMQAT

151 ATSGFASGME DKHSSDASSL LPQNILSQTS RHNDRDYRLP RAETHSSSTP

201 VQHPIKPVVH PTATPSTVPS SPFTLQSDHQ PKKSFDANGA STLSKLPTPT

251 SSVPAQKTER KESTSGDKPV SHSCTTPSTS SASGLNPTSA PPTSASAVPV

301 SPVPQSPIPP LLQDPNLLRQ LLPALQATLQ LNNSNVDISK INEVLTAAVT

351 QASLQSIIHK FLTAGPSAFN ITSLISQAAQ LSTQDIPLHE GIQMERDTHR

401 SKWEVKGSLC QKADKQQECL VWNGSIMVQR LLQPSG

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_19j 17, frame 3

TREMBL :CEY40B1A_2 gene: "Y40B1A.2"; Caenorhabditis elegans cosmid Y40B1A, N = 1, Score = 144, P = 1.8e-09

>TREMBL:CEY40B1A_2 gene: "Y40B1A.2"; Caenorhabditis elegans cosmid Y40B1A Length = 120

HSPs:

Score = 144 (21.6 bits), Expect = 1.8e-09, P = 1.8e-09 Identities = 30/67 (44%), Positives = 43/67 (64%)

Query: 90 WSEHISSSGKKYYYNCRTEVSQWEKPKEW-LEREQRQKEANKMAVNSFPK DRDYRRE 145

W+E +SSSGK YYYN +TE+SQW+KP EW E +++ K VN P+ DR Y Sbjct: 11 WTEQMSSSGKMYYYNKKTEISQWDKPAEWPAEGGSAERDKPKGGVNEKPRFAEDR-YNEY 69

Query: 146 VMQATATS 153

+ Q +++S Sbjct: 70 IGQLΞSSS 77

Pedant information for DKFZphtes3_19j 17, frame 2

Report for DKFZphtes3_19jl7.2

[LENGTH] 209

[MW] 22873.85

[pi] 9.95

[KW] All_Alpha

[KW] LOW_COMPLEXITY 13.40 %

SEQ MSLTSDASSPRSYVSPRISTPQTNTVPIKPLISTPPVSSQPKVSTPVVKQGPVSQSATQQ SEG

PRD ccccccccccccccccccccccceeeeccccccccccccccccccceeeccccccccccc

SEQ PVTADKQQGHEPVSPRSLQRΞSQRSPSPGPNHTSNSSNASNATVVPQNSSARSTCSLTPA

SEG xxxxxxxxxxxxxxx..xxxxxxxxxxxxx

PRD cccccccccccccccccccccccccccccccccccccccccceeeeccccccccccchhh

SEQ LAAHFSENLIKHVQGWPADHAEKQASRLREEAHNMGTIHMSEICTELKNLRSLVRVCEIQ SEG

PRD hhhhhhcchhhhhhccccchhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhhh

SEQ ATLREQRILFLRQQIKELEKLKNQNSFMV SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhcccccc

(No Prosite data available for DKFZphtes3_19j 17.2) (No Pfam data available for DKFZphtes3_19j 17.2)

Pedant information for DKFZphtes3_19j 17, frame 3

Report for DKFZphtes3_19 l7.3

[LENGTH] 436

[MW] 47716.62

[pi] 8.71

[HOMOL] TREMBL:CEY40B1A_2 gene: "Y40B1A.2"; Caenorhabditis elegans cosmid Y40B1A 6e-0S

[FUNCAT] 04.05.03 mrna processing (splicing) [Ξ. cerevisiae, YKL012w] 2e-04

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKL012w] 2e-04

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YPR152c] 6e-04

[BLOCKS] BL01159 WW/rsp5/WWP domain proteins

[PROSITE] WW_DOMAIN_l 2

[PFAM] WW/rsp5/WWP domain containing proteins

[KW] All_Alpha

[KW] LOW COMPLEXITY 22.48 % SEQ MRDAGDPSPPNKMLRRSDSPENKYSDSTGHSKAKNVHTHRVRERDGGTSYSPQENSHNHS

SEG xxxxxx

PRD ccccccccccccccccccccccccccccccccccccceeeeeeccccccccccccccccc

SEQ ALHSSNSHSSNPSNNPSKTSDAPYDSADDWSEHISSSGKKYYYNCRTEVSQWEKPKEWLE

SEG xxxxxxxxxxxxxxxxx

PRD ccccccccccccccccccccccccccccccceeeccccceeeeeeccccccccccchhhh

SEQ REQRQKEANKMAVNSFPKDRDYRREVMQATATSGFASGMEDKHSSDASSLLPQNILSQTS

SEG

PRD hhhhhhhhhhhhcccccccchhhhhhhhhhcccccccccccccccccccccccccccccc

SEQ RHNDRDYRLPRAETHSSSTPVQHPIKPVVHPTATPSTVPSSPFTLQSDHQPKKSFDANGA

SEG xxxxxxxxxxxxxxxx

PRD ccccccccccccccccccccccccceeeeccccccccccccccccccccccccccccccc

SEQ STLSKLPTPTSSVPAQKTERKESTSGDKPVSHSCTTPSTSSASGLNPTSAPPTSASAVPV

SEG xxxxxxxxxxxx xxxxxxxxxxxxxx

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ SPVPQSPIPPLLQDPNLLRQLLPALQATLQLNNSNVDIΞKINEVLTAAVTQASLQSIIHK

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD cccccccccccccccchhhhhhhhhhhhhhhcccccccccchhhhhhhhhhhhhhhhhhh

SEQ FLTAGPSAFNITSLISQAAQLΞTQDIPLHEGIQMERDTHRSKWEVKGSLCQKADKQQECL

SEG

PRD hhcccccceeehhhhhhhhhhhccccccccccccccccccceeeecccchhhhhhhccee

SEQ VWNGSIMVQRLLQPSG

SEG

PRD eeccchhhhhhccccc

Prosite for DKFZphtes3_19j 17.3

PS01159 90->116 WW_DOMAIN_l PDOC50020

PS01159 90->116 WW DOMAIN 1 PDOC50020

Pfam for DKFZphtes3_19j 17.3

HMM_NAME WW/rsp5/WWP domain containing proteins

HMM *LPsGWEeHWDpsGRpWYYWNHETkTTQWEpP*

+ ++W EH++ SG+ YY+N T+ +QWE+P Query 86 SADDWSEHISSSGKK-YYYNCRTEVΞQWEKP 115

DKFZphtes3_lcl

group : signal transduction

DKFZphtes3_lcl encodes a novel 632 amino acid putative GTPase-activating protein, related to drosophila rotund transcript and human n-chimaerin . rac small GTPase is associated with type-I phosphatidylinositol 4-phosphate 5-kιnase and regulating the production of phosphatidylinositol 4 , 5-bιsphosphate . The new protein is expected to activate p21rac-related small GTPases .

The new protein can find application in modulating/blocking the response to a cellular receptor . similarity to GTPase-activating proteins complete cDNA, complete eds , EST hits

Sequenced by DKFZ

Locus : unknown

Insert length : 3237 bp

Poly A stretch at pos . 3227 , no polyadenylation signal found

1 GCGAAGTGAA GGGTGGCCCA GGTGGGGCCA GGCTGACTGA ATGTATCTCC

51 TAGCTATGGA CTAAATAATA CATGGGGGGA AATAAACAAG TATTCATGAG

101 GGTGAAAATG TGACCCAGCA GGAAAATTAC AACTATTTTC AATTGACGTT

151 GAATAGGATG AGTCATGGAA TTTAAGTGAT TTACTGAAGA TTATACTACT

201 GGTAGATAGA AGAGCTAAAG AAAGATGGAT ACTATGATGC TGAATGTGCG

251 GAATCTGTTT GAGCAGCTTG TGCGCCGGGT GGAGATTCTC AGTGAAGGAA

301 ATGAAGTCCA ATTTATCCAG TTGGCGAAGG ACTTTGAGGA TTTCCGTAAA

351 AAGTGGCAGA GGACTGACCA TGAGCTGGGG AAATACAAGG ATCTTTTGAT

401 GAAAGCAGAG ACTGAGCGAA GTGCTCTGGA TGTTAAGCTG AAGCATGCAC

451 GTAATCAGGT GGATGTAGAG ATCAAACGGA GACAGAGAGC TGAGGCTGAC

501 TGCGAAAAGC TGGAACGACA GATTCAGCTG ATTCGAGAGA TGCTCATGTG

551 TGACACATCT GGCAGCATTC AACTAAGCGA GGAGCAAAAA TCAGCTCTGG

601 CTTTTCTCAA CAGAGGCCAA CCATCCAGCA GCAATGCTGG GAACAAAAGA

651 CTATCAACCA TTGATGAATC TGGTTCCATT TTATCAGATA TCAGCTTTGA

701 CAAGACTGAT GAATCACTGG ATTGGGACTC TTCTTTGGTG AAGACTTTCA

751 AACTGAAGAA GAGAGAAAAG AGGCGCTCTA CTAGCCGACA GTTTGTTGAT

801 GGTCCCCCTG GACCTGTAAA GAAAACTCGT TCCATTGGCT CTGCAGTAGA

851 CCAGGGGAAT GAATCCATAG TTGCAAAAAC TACAGTGACT GTTCCCAATG

901 ATGGCGGGCC CATCGAAGCT GTGTCCACTA TTGAGACTGT GCCATATTGG

951 ACCAGGAGCC GAAGGAAAAC AGGTACTTTA CAACCTTGGA ACAGTGACTC

1001 CACCCTGAAC AGCAGGCAGC TGGAGCCAAG AACTGAGACA GACAGTGTGG

1051 GCACGCCACA GAGTAATGGA GGGATGCGCC TGCATGACTT TGTTTCTAAG

1101 ACGGTTATTA AACCTGAATC CTGTGTTCCA TGTGGAAAGC GGATAAAATT

1151 TGGCAAATTA TCTCTGAAGT GTCGAGACTG TCGTGTGGTC TCTCATCCAG

1201 AATGTCGGGA CCGCTGTCCC CTTCCCTGCA TTCCTACCCT GATAGGAACA

1251 CCTGTCAAGA TTGGAGAGGG AATGCTGGCA GACTTTGTGT CCCAGACTTC

1301 TCCAATGATC CCCTCCATTG TTGTGCATTG TGTAAATGAG ATTGAGCAAA

1351 GAGGTCTGAC TGAGACAGGC CTGTATAGGA TCTCTGGCTG TGACCGCACA

1401 GTAAAAGAGC TGAAAGAGAA ATTCCTCAGA GTGAAAACTG TACCCCTCCT

1451 CAGCAAAGTG GATGATATCC ATGCTATCTG TAGCCTTCTA AAAGACTTTC

1501 TTCGAAACCT CAAAGAACCT CTTCTGACCT TTCGCCTTAA CAGAGCCTTT

1551 ATGGAAGCAG CAGAAATCAC AGATGAAGAC AACAGCATAG CTGCCATGTA

1601 CCAAGCTGTT GGTGAACTGC CCCAGGCCAA CAGGGACACA TTAGCTTTCC

1651 TCATGATTCA CTTGCAGAGA GTGGCTCAGA GTCCACATAC TAAAATGGAT

1701 GTTGCCAATC TGGCTAAAGT CTTTGGCCCT ACAATAGTGG CCCATGCTGT

1751 GCCCAATCCA GACCCAGTGA CAATGTTACA GGACATCAAG CGTCAACCCA

1801 AGGTGGTTGA GCGCCTGCTT TCCTTGCCTC TGGAGTATTG GAGTCAGTTC

1851 ATGATGGTGG AGCAAGAGAA CATTGACCCC CTACATGTCA TTGAAAACTC

1901 AAATGCCTTT TCAACACCAC AGACACCAGA TATTAAAGTG AGTTTACTGG

1951 GACCTGTGAC CACTCCTGAA CATCAGCTTC TCAAGACTCC TTCATCTAGT

2001 TCCCTGTCAC AGAGAGTCCG TTCCACCCTC ACCAAGAACA CTCCTAGATT

2051 TGGGAGCAAA AGCAAGTCTG CCACTAACCT AGGACGACAA GGCAACTTTT

2101 TTGCTTCTCC AATGCTCAAG TGAAGTCACA TCTGCCTGTT ACTTCCCAGC

2151 ATTGACTGAC TATAAGAAAG GACACATCTG TACTCTGCTC TGCAGCCTCC

2201 TGTACTCATT ACTACTTTTA GCATTCTCCA GGCTTTTACT CAAGTTTAAT

2251 TGTGCATGAG GGTTTTATTA AAACTATATA TATCTCCCCT TCCTTCTCCT

2301 CAAGTCACAT AATATCAGCA CTTTGTGCTG GTCATTGTTG GGAGCTTTTA

2351 GATGAGACAT CTTTCCAGGG GTAGAAGGGT TAGTATGGAA TTGGTTGTGA

2401 TTCTTTTTGG GGAAGGGGGT TATTGTTCCT TTGGCTTAAA GCCAAATGCT

2451 GCTCATAGAA TGATCTTTCT CTAGTTTCAT TTAGAACTGA TTTCCGTGAG

2501 ACAATGACAG AAACCCTACC TATCTGATAA GATTAGCTTG TCTCAGGGTG

2551 GGAAGTGGGA GGGCAGGGCA AAGAAAGGAT TAGACCAGAG GATTTAGGAT 2601 GCCTCCTTCT AAGAACCAGA AGTTCTCATT CCCCATTATG AACTGAGCTA

2651 TAATATGGAG CTTTCATAAA AATGGGATGC ATTGAGGACA GAACTAGTGA

2701 TGGGAGTATG CGTAGCTTTG ATTTGGATGA TTAGGTCTTT AATAGTGTTG

2751 AGTGGCACAA CCTTGTAAAT GTGAAAGTAC AACTCGTATT TATCTCTGAT

2801 GTGCCGCTGG CTGAACTTTG GGTTCATTTG GGGTCAAAGC CAGTTTTTCT

2851 TTTAAAATTG AATTCATTCT GATGCTTGGC CCCCATACCC CCAACCTTGT

2901 CCAGTGGAGC CCAACTTCTA AAGGTCAATA TATCATCCTT TGGCATCCCA

2951 ACTAACAATA AAGAGTAGGC TATAAGGGAA GATTGTCAAT ATTTTGTGGT

3001 AAGAAAAGCT ACAGTCATTT TTTCTTTGCA CTTTGGATGC TGAAATTTTT

3051 CCCATGGAAC ATAGCCACAT CTAGATAGAT GTGAGCTTTT TCTTCTGTTA

3101 AAATTATTCT TAATGTCTGT AAAAACGATT TTCTTCTGTA GAATGTTTGA

3151 CTTCGTATTG ACCCTTATCT GTAAAACACC TATTTGGGAT AATATTTGGA

3201 AAAAAAGTAA ATAGCTTTTT CAAAATGAAA AAAAAAA

BLAST Results

Entry U82984 from database EMBLEST :

Homo sapiens DRES 56 mRNA sequence.

Score = 8775, P = O.Oe+00, identities = 1757/1758 matches 3 ' end

Medline entries

93074974:

Developmental regulation and neuronal expression of the mRNA of rat n-chimaeπn, a p21rac GAP:cDNA sequence.

93024458:

A Drosophila rotund transcript expressed during spermatogenesis and imaginal disc morphogenesis encodes a protein which is similar to human Rac

GTPase-activating

(racGAP) proteins.

Peptide information for frame 3

ORF from 225 bp to 2120 bp; peptide length: 632 Category: similarity to known protein

1 MDTMMLNVRN LFEQLVRRVE ILSEGNEVQF IQLAKDFEDF RKKWQRTDHE

51 LGKYKDLLMK AETERSALDV KLKHARNQVD VEIKRRQRAE ADCEKLERQI

101 QLIREMLMCD TSGSIQLSEE QKSALAFLNR GQPSSSNAGN KRLSTIDESG

151 SILSDISFDK TDESLDWDSS LVKTFKLKKR EKRRSTSRQF VDGPPGPVKK

201 TRSIGSAVDQ GNESIVAKTT VTVPNDGGPI EAVSTIETVP YWTRSRRKTG

251 TLQPWNSDST LNSRQLEPRT ETDSVGTPQS NGGMRLHDFV SKTVIKPESC

301 VPCGKRIKFG KLSLKCRDCR VVSHPECRDR CPLPCIPTLI GTPVKIGEGM

351 LADFVSQTSP MIPSIVVHCV NEIEQRGLTE TGLYRISGCD RTVKELKEKF

401 LRVKTVPLLS KVDDIHAICS LLKDFLRNLK EPLLTFRLNR AFMEAAEITD

451 EDNSIAAMYQ AVGELPQANR DTLAFLMIHL QRVAQSPHTK MDVANLAKVF

501 GPTIVAHAVP NPDPVTMLQD IKRQPKVVER LLSLPLEYWS QFMMVEQENI

551 DPLHVIENSN AFSTPQTPDI KVSLLGPVTT PEHQLLKTPS SSSLSQRVRS

601 TLTKNTPRFG SKSKSATNLG RQGNFFASPM LK

BLASTP hits

Entry CEK08E3_4 from database TREMBLNEW: gene: "K08E3.6"; Caenorhabditis elegans cosmid K08E3

Score = 452, P = 2.6e-48, identities = 126/377, positives = 189/377

Entry A48122 from database PIR:

GTPase-activatmg protein Rac homolog, splice form clone pel.7 - fruit fly (Drosophila melanogaster) (fragment)

Score = 480, P = 9.2e-46, identities = 111/270, positives = 155/270

Entry B48122 from database PIR:

GTPase-activat g protein Rac homolog, splice form clone pcl.7d - fruit fly (Drosophila melanogaster)

Score = 480, P = 9.2e-46, identities = 111/270, positives = 155/270 Entry DM22539_1 from database TREMBL: gene: "rotund"; product: "rnracGAP"; Drosophila melanogaster rnracGAP

(rotund) gene, complete eds.

Score = 480, P = 9.2e-46, identities = 111/270, positives = 155/270

Entry S29128 from database PIR:

N-chimerm - rat

Score = 336, P = 8.8e-30, identities = 86/253, positives 128/253

Alert BLASTP hits for DKFZphtes3_lcl, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphtes3_lcl, frame 3

Report for DKFZphtes3_lcl .3

[LENGTH] 632

[MW] 71026.84

[pi] 9.08

[HOMOL] PIR:B48122 GTPase-activatmg protein Rac homolog, splice form clone pcl.7d - fruit fly (Drosophila melanogaster) 2e-46

[FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YBR260C] 3e-12

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YER155C] 2e-ll

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YER155C] 2e-ll

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YER155c]

2e-ll

[FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YDL240w] 3e-09

[FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YOR134w] 4e-09

[ FUNCAT ] 06.10 assembly of protein complexes [S. cerevisiae, YOR134w] 4e-09

[FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins

[S. cerevisiae, YOR127w] 5e-09 [FUNCAT] 09.04 biogenesis of cytoskeleton [S. cerevisiae, YPL115C] 3e-08 [FUNCAT] 10.02.09 regulation of g-protein activity [S. cerevisiae, YPL115c] 3e-08 [BLOCKS] BL00479B Phorbol esters / diacylglycerol binding domain proteins [BLOCKS] BL00479A Phorbol esters / diacylglycerol binding domain proteins [SCOP] dlpbwa_ 1.83.1.1.2 p85 alpha subunit RhoGAP domain [human (Hom le-55 [SCOP] dlrgp 1.83.1.1.1 p50 RhoGAP domain human (Homo sapiens) le-49 [PIRKW] breakpoint cluster region le-19 [PIRKW] transmembrane protein 7e-08 [PIRKW] brain 3e-22 [PIRKW] alternative splicing le-19 [PIRKW] P-loop 2e-25 [SUPFAM] CDC24 homology 3e-22 [SUPFAM] bcr protein 3e-22 [SUPFAM] myosin motor domain homology 2e-25 [SUPFAM] pleckstrm repeat homology 4e-10 [SUPFAM] LIM metal-b dmg repeat homology 2e- 09 [SUPFAM] protem kinase C zmc-binding repeat homology 5e-29 [PROSITE] MYRISTYL 6 [PROSITE] AMIDATION 1 [PROSITE] CAMP_PHOSPHO_SITE 3 [PROSITE] CK2_PHOSPHO_SITE 13 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SI E 9 [PROSITE] ASN_GLYCOSYLATION 1 [PROSITE] DAG PE BINDING DOMAIN 1 [PFAM] Phorbol esters / diacylglycerol binding domain [KW] Irregular [KW] 3D [KW] LOW_COMPLEXITY 2.22 % [KW] COILED COIL 8.54 %

SEQ MDTMMLNVRNLFEQLVRRVEILSEGNEVQFIQLAKDFEDFRKKWQRTDHELGKYKDLLMK

SEG

COILS cccccccccccc

Irgp-

SEQ AETERSALDVKLKHARNQVDVEIKRRQRAEADCEKLERQIQLIREMLMCDTSGSIQLΞEE

SEG

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

Irgp-

SEQ QKSALAFLNRGQPSSSNAGNKRLSTIDESGSILΞDISFDKTDESLDWDSSLVKTFKLKKR

SEG

COILS irgp-

SEQ EKRRSTSRQFVDGPPGPVKKTRΞIGSAVDQGNEΞIVAKTTVTVPNDGGPIEAVSTIETVP

SEG

COILS irgp-

SEQ YWTRΞRRKTGTLQPWNSDSTLNSRQLEPRTETDSVGTPQSNGGMRLHDFVSKTVIKPESC

SEG

COILS

Irgp-

SEQ VPCGKRIKFGKLSLKCRDCRVVSHPECRDRCPLPCIPTLIGTPVKIGEGMLADFVSQTSP

SEG

COILS irgp-

SEQ MIPSIVVHCVNEIEQRGLTETGLYRISGCDRTVKELKEKFLRVKTVPLLSKVDDIHAICS

SEG

COILS

Irgp- .CCHHHHHHHHHHHHHHTTTTTTTTTCCCHHHHHHHHHHHHHCCCCCG-GGCCCCHHHHH

SEQ LLKDFLRNLKEPLLTFRLNRAFMEAAEITDEDNSIAAMYQAVGELPQANRDTLAFLMIHL

SEG

COILS

Irgp- HHHHHHHHTTTTTTTGGGHHHHHHTTTT-CGGGHHHHHHHHHHHCCHHHHHHHHHHHHHH

SEQ QRVAQSPHTKMDVANLAKVFGPTIVAHAVPNPDPVTMLQDIKRQPKVVERLLSLPLEYWS

SEG

COILS

Irgp- HHHHHHHHHCCCHHHHHHHHGGGCC

SEQ QFMMVEQENIDPLHVIENSNAFSTPQTPDIKVSLLGPVTTPEHQLLKTPSSSSLSQRVRS

SEG xxxxxxxxxxx

COILS irgp-

SEQ TLTKNTPRFGSKSKSATNLGRQGNFFASPMLK

SEG xxx

COILS irgp-

Prosite for DKFZphtes3_lcl .3

PS00001 212->216 ASN_GLYCOSYLATION PDOC00001 PS00004 141->145 CAMP_PHOSPHO_SITE PDOC00004 PS00004 182->186 CAMP_PHOSPHO_SITE PDOC00004 PS00004 246->250 CAMP_PHOSPHO_SITE PDOC00004 PS00005 63->66 PKC_PHOSPHO_SITE PDOC00005 PS00005 174->177 PKC_PHOSPHO_SITE PDOC00005 PS00005 186->189 PKC_PHOSPHO_SITE PDOC00005 PS00005 245->248 PKC_PHOSPHO_SITE PDOC00005 PS00005 313->316 PKC_PHOSPHO_SITE PDOC00005 PS00005 392->395 PKC_PHOSPHO_SITE PDOC00005 PS00005 435->438 PKC_PHOSPHO_SITE PDOC00005 PS00005 595->598 PKC_PHOSPHO_SITE PDOC00005 PS00005 606->609 PKC_PHOSPHO_SITE PDOC00005 PS00006 47->51 CK2_PHOSPHO_SITE PDOC00006 PS00006 66->70 CK2_PHOSPHO_SITE PDOC00006 PS00006 144->148 CK2_PHOΞPHO_SITE PDOC00006 PS00006 206->210 CK2_PHOSPHO_SITE PDOC00006 PS00006 234->238 CK2_PHOSPHO_SITE PDOC00006 PS00006 270->274 CK2_PHOSPHO_SITE PDOC00006 PS00006 323->327 CK2_PHOSPHO_SITE PDOC00006 PS00006 387->391 CK2_PHOSPHO_SITE PDOC00006 PS00006 392->396 CK2_PHOSPHO_SITE PDOC00006 PS00006 410->414 CK2_PHOSPHO_SITE PDOC00006 PS00006 449->453 CK2_PHOSPHO_SITE PDOC00006 PS00006 489->493 CK2_PHOSPHO_SITE PDOC00006 PS00006 579->583 CK2_PHOSPHO_SITE PDOC00006 PS00007 46->55 TYR_PHOSPHO_SITE PDOC00007 PS00007 376->385 TYR_PHOSPHO_SITE PDOC00007 PS00008 131->137 MYRISTYL PDOC00008 PS00008 150->156 MYRISTYL PDOC00008 PS00008 276->282 MYRISTYL PDOC00008 PS00008 377->383 MYRISTYL PDOC00008 PS00008 388->394 MYRISTYL PDOC00008 PS00008 623->629 MYRISTYL PDOC00008 PS00009 303->307 AMIDATION PDOC00009 PS00479 287->336 DAG PE BINDING DOMAIN PDOC00379

Pfam for DKFZphtes3_lcl .3

HMM_NAME Phorbol esters / diacylglycerol binding domain

HMM *HrFmrHTFrqPTWCDHCgeFIWGWgKQGYQCQnCgMNCHKRCHelVPmm

H+F+ +T + P +C CG +1 +GK ++C +C+++ H +C+ + P Query 287 HDFVSKTVIKPESCVPCGKRI-KFGKLSLKCRDCRVVSHPECRDRCPLP 334

HMM C*

C Query 335 C 335

DKFZphtes3_lgl3

group: intracellular transport and trafficking

DKFZp DKFZphtes3_lgl3 encodes a novel 1007 amino acid protein with similarity to human 256 kD golgin.

The new protein contains 7 leucme zippers and seems to be involved in protein-protein- mteraction in the golgi apparatus. The very similar rat cpl51 shows haploid-specific transcription in mus musculus testis.

The new protein can find application m modulating protein traffic in the golgi apparatus, especially in human haploid germ cells. similarity to 256 kD golgi, strong similarity to rat "cpl51"

21 exons encoded on AC004682

EST from a testis library, two mouse ESTs of a testis cDNA library, rat cpl51 shows haploid-specific transcription¹ testis or haploid-specific transcription

Sequenced by DKFZ

Locus: map="16q22.2"

Insert length: 3405 bp

Poly A stretch at pos. 3394, polyadenylation signal at pos. 3373

1 GGGATAGGGG ATGTGGTTTG TTACAAAGGA TGAGTATTTT GATAGCTTCT 51 CATTCCTTGA ACTATTCTGC AGGTTTATAA CAAAGCTCAG AAAATACTAA

101 AGGTTAAAGG AGAATTGAGA GCTGCCAAGG AAATGAAAGA TGAGGCGGGG

151 GAGAGAGACA GAGAAGTGAG CAGCCTGAAC AGCAAGCTGT TAAGCCTGCA

201 ACTTGACATC AAGAATCTGC ACGATGTCTG CAAGAGACAG AGGAAGACCT

251 TGCAGGACAA TCAGCTCTGC ATGGAGGAGG CAATGAACAG CAGCCACGAC

301 AAGAAGCAAG CACAGGCATT AGCATTCGAG GAGTCAGAGG TGGAATTTGG

351 GTCCAGTAAA CAGTGTCATC TGAGACAACT CCAGCAACTG AAGAAAAAAT

401 TGCTGGTCCT TCAACAAGAA CTGGAGTTTC ACACAGAGGA GTTGCAGACT

451 TCTTACTATT CTCTCCGCCA GTATCAGTCC ATCCTAGAGA AGCAGACTTC

501 CGACCTGGTT CTTCTGCACC ATCACTGCAA ACTGAAAGAA GATGAGGTGA

551 TTCTCTATGA GGAGGAAATG GGAAATCACA ACGAGAACAC AGGGGAGAAG

601 CTCCATTTGG CGCAGGAGCA ACTCGCCTTG GCCGGGGACA AGATCGCCTC

651 TCTAGAGAGG AGCTTAAACC TCTACAGGGA TAAATACCAG TCTTCCCTGA

701 GCAACATCGA GTTACTAGAA TGCCAAGTGA AGATGTTGCA GGGGGAACTC

751 GGCGGGATCA TGGGTCAGGA GCCTGAGAAC AAGGGTGATC ATTCAAAGGT

801 ACGGATATAC ACTTCTCCTT GCATGATTCA AGAGCATCAG GAGACTCAGA

851 AACGACTGTC TGAAGTCTGG CAAAAGGTCT CTCAACAGGA TGATCTCATT

901 CAAGAACTTC GAAATAAGCT GGCCTGCAGT AACGCTTTGG TTCTGGAGCG

951 TGAAAAGGCT TTGATAAAAC TACAAGCCGA TTTTGCTTCC TGTACAGCCA 1001 CCCACAGATA CCCTCCTAGC TCCTCAGAAG AGTGTGAAGA CATCAAAAAG 1051 ATACTGAAGC ACTTGCAGGA GCAGAAAGAC AGCCAGTGCC TGCATGTGGA 1101 GGAGTACCAG AACCTGGTGA AGGATCTGCG CGTGGAACTA GAGGCCGTGT 1151 CGGAACAGAA GAGAAACATC ATGAAGGACA TGATGAAGCT GGAGCTGGAC 1201 CTGCACGGAC TGCGGGAGGA GACATCTGCC CACATTGAGA GGAAGGATAA 1251 GGACATCACC ATCCTGCAGT GCCGGCTGCA GGAGCTGCAG CTGGAGTTCA 1301 CCGAGACCCA AAAGCTCACT TTGAAGAAAG ACAAGTTCCT CCAAGAGAAA 1351 GATGAGATGC TGCAAGAGCT GGAGAAGAAA CTGACACAGG TTCAGAACAG 1401 CCTCCTGAAA AAGGAGAAGG AGCTGGAGAA GCAGCAGTGC ATGGCCACAG 1451 AACTTGAAAT GACAGTCAAG GAGGCTAAGC AGGACAAGTC CAAGGAGGCG 1501 GAGTGCAAGG CCCTGCAGGC TGAGGTCCAG AAGCTGAAGA ACAGTCTCGA 1551 AGAGGCCAAG CAGCAGGAGA GGCTGGCTGC TCAGCAAGCA GCCCAGTGCA 1601 AAGAAGAGGC TGCACTGGCA GGCTGTCACC TGGAGGACAC CCAGAGGAAA 1651 CTGCAGAAGG GTCTCCTCCT GGACAAGCAG AAGGCAGACA CCATCCAGGA 1701 ACTACAGAGA GAACTTCAGA TGCTGCAGAA GGAGTCCTCG ATGGCTGAGA 1751 AGGAACAAAC CTCCAACAGA AAACGGGTGG AGGAGCTGTC ATTAGAACTC 1801 TCTGAAGCCC TGAGGAAGCT TGAAAATTCA GACAAGGAAA AGAGGCAGCT 1851 TCAGAAGACA GTGGCTGAGC AGGATATGAA AATGAATGAC ATGCTTGATC 1901 GTATCAAGCA CCAGCACAGG GAGCAAGGCT CCATCAAATG CAAGTTAGAA 1951 GAAGATCTTC AGGAGGCCAC AAAGCTTCTG GAGGACAAAC GGGAGCAGTT 2001 GAAGAAGAGC AAAGAGCATG AGAAGCTGAT GGAGGGAGAA CTTGAAGCTT 2051 TGCGGCAGGA ATTTAAAAAG AAAGACAAGA CGTTGAAAGA GAATTCCAGA 2101 AAGTTGGAGG AAGAAAATGA GAATCTCCGA GCAGAGCTAC AGTGTTGTTC 2151 TACACAACTG GAATCCTCTC TCAACAAATA CAACACCAGC CAGCAAGTCA 2201 TCCAAGACTT GAATAAAGAG ATAGCCCTTC AGAAGGAGTC CTTAATGAGC 2251 CTGCAGGCCC AGCTGGACAA AGCTCTGCAG AAGGAGAAGC ACTATCTCCA 2301 GACTACCATC ACCAAAGAAG CCTATGATGC ATTATCCCGG AAGTCAGCCG 2351 CCTGCCAGGA TGACCTGACA CAAGCCCTCG AGAAGCTCAA TCACGTGACC 2401 TCAGAGACAA AGAGCCTGCA GCAAAGCTTG ACACAGACCC AAGAGAAGAA 2451 AGCTCAGCTG GAAGAGGAAA TCATTGCTTA TGAGGAAAGG ATGAAAAAGC

2501 TCAATACGGA ATTAAGAAAA CTGCGGGGCT TCCACCAGGA GAGTGAGCTG

2551 GAGGTGCACG CCTTTGACAA GAAGCTAGAG GAGATGAGCT GCCAGGTGCT

2601 GCAGTGGCAG AAGCAACACC AGAATGACCT CAAGATGCTG GCAGCCAAAG

2651 AGGAGCAGCT CAGGGAGTTC CAGGAGGAGA TGGCCGCCTT AAAAGAGAAC

2701 CTCCTTGAGG ACGATAAGGA GCCCTGCTGC CTGCCCCAGT GGTCTGTGCC

2751 CAAAGACACC TGTAGGCTCT ACCGAGGGAA TGATCAGATT ATGACCAACT

2801 TGGAGCAATG GGCAAAACAG CAGAAGGTCG CCAATGAGAA ACTAGGAAAC

2851 CAGCTCCGAG AGCAGGTGAA CTACATTGCC AAGCTGAGTG GCGAAAAGGA

2901 CCACCTCCAC AGTGTAATGG TCCACTTGCA GCAGGAAAAC AAGAAGCTGA

2951 AGAAGGAGAT AGAAGAGAAG AAGATGAAAG CCGAGAACAC AAGGCTATGC

3001 ACCAAAGCCC TAGGCCCGAG CAGAACGGAG TCCACACAGA GGGAGAAAGT

3051 GTGCGGCACC TTGGGCTGGA AGGGGTTGCC CCAGGATATG GGTCAAAGAA

3101 TGGACCTCAC CAAGTACATC GGGATGCCCC ACTGCCCGGG TTCCTCATAC

3151 TGCTAGAATC CACATCTAGC CCTGAGCAGC ATTTCCACGG GTGTTTCTTC

3201 AGAGGACAGT GAGTTCCCAG CCCTCCCTCT CTCTTGACCT GGATCAGCTC

3251 TTACAGGAGT ATATCACGGT CCCAGCCTAT TTTGCAAGAC ACTAACTTTT

3301 GTTGAGTTTT GTCCACTTCC TGCCATGGAG TGAGCTTTAG AACCATACTA

3351 CCATCTCCAG GCCCAAACTC TGAAATAAAG ACATGAGCAT GAGCAAAAAA

3401 AAAAA

BLAST Results

Entry AC004682 from database EMBLNEW:

Homo sapiens Chromosome 16 BAC clone CIT987SK-A-259H10, complete sequence . Score = 1291, P = O.Oe+00, identities = 265/272

Medline entries

No Medline entry

Peptide information for frame 1

ORF from 133 bp to 3153 bp; peptide length: 1007

Category: similarity to known protein

Prosite motifs: LEUCINE_ZIPPER (83-105)

LEUCINE_ZIPPER (90-112)

LEUCINE_ZIPPER (97-119)

LEUCINE_ZIPPER (104-126)

LEUCINE_ZIPPER (403-425)

LEUCINE_ZIPPER (410-432)

LEUCINE ZIPPER (918-940)

1 MKDEAGERDR EVSSLNSKLL SLQLDIKNLH DVCKRQRKTL QDNQLCMEEA 51 MNSSHDKKQA QALAFEESEV EFGΞSKQCHL RQLQQLKKKL LVLQQELEFH 101 TEELQTSYYS LRQYQSILEK QTSDLVLLHH HCKLKEDEVI LYEEEMGNHN 151 ENTGEKLHLA QEQLALAGDK IASLERSLNL YRDKYQSSLS NIELLECQVK 201 MLQGELGGIM GQEPENKGDH SKVRIYTSPC MIQEHQETQK RLSEVWQKVS 251 QQDDLIQELR NKLACSNALV LEREKALIKL QADFASCTAT HRYPPSSSEE 301 CEDIKKILKH LQEQKDSQCL HVEEYQNLVK DLRVELEAVS EQKRNIMKDM 351 MKLELDLHGL REETSAHIER KDKDITILQC RLQELQLEFT ETQKLTLKKD 401 KFLQEKDEML QELEKKLTQV QNSLLKKEKE LEKQQCMATE LEMTVKEAKQ 451 DKSKEAECKA LQAEVQKLKN SLEEAKQQER LAAQQAAQCK EEAALAGCHL 501 EDTQRKLQKG LLLDKQKADT IQELQRELQM LQKESSMAEK EQTΞNRKRVE 551 ELSLELSEAL RKLENSDKEK RQLQKTVAEQ DMKMNDMLDR IKHQHREQGS 601 IKCKLEEDLQ EATKLLEDKR EQLKKSKEHE KLMEGELEAL RQEFKKKDKT 651 LKENSRKLEE ENENLRAELQ CCSTQLESSL NKYNTSQQVI QDLNKEIALQ 701 KESLMSLQAQ LDKALQKEKH YLQTTITKEA YDALSRKSAA CQDDLTQALE 751 KLNHVTSETK SLQQSLTQTQ EKKAQLEEEI IAYEERMKKL NTELRKLRGF 801 HQESELEVHA FDKKLEEMSC QVLQWQKQHQ NDLKMLAAKE EQLREFQEEM 851 AALKENLLED DKEPCCLPQW SVPKDTCRLY RGNDQIMTNL EQWAKQQKVA 901 NEKLGNQLRE QVNYIAKLSG EKDHLHSVMV HLQQENKKLK KEIEEKKMKA 951 ENTRLCTKAL GPSRTESTQR EKVCGTLGWK GLPQDMGQRM DLTKYIGMPH 1001 CPGSSYC

BLASTP hits

Entry HS417401_1 from database TREMBL: product: "trans-Golgi p230"; Human trans-Golgi p230 mRNA, complete eds .

Score = 411, P = 3.9e-34, identities = 212/862, positives = 420/862

Entry SCINTANA_1 from database TREMBL:

Saccharomyces cerevisiae integrin analogue gene, complete eds.

Score = 404, P = 6.2e-34, identities = 199/897, positives = 423/897

Entry HS6802_2 from database TREMBL: gene: "MYH9"; product: "dJ6802.2"; Homo sapiens DNA sequence from PAC

6802 on chromosome 22. Contains apolipoprotein L, myosin heavy chain,

ESTs, CA repeat, STS and GSS.

Score = 404, P = 1.9e-33, identities = 231/1028, positives = 469/1028

Entry AF092090_1 from database TREMBL: product: "cpl51"; Rattus norvegicus cpl51 mRNA, partial eds.

Score = 2523, P = 3.0e-262, identities = 506/733, positives = 611/733

Alert BLASTP hits for DKFZphtes3_lgl3, frame 1

TREMBL :HSGOLGIN_l product: "256 kD golgin"; H. sapiens mRNA for golgm, N = 1, Score = 411, P = 4.4e-34

TREMBL:HS417401_1 product: "trans-Golgi p230"; Human trans-Golgi p230 mRNA, complete eds., N = 1, Score = 411, P = 4.5e-34

TREMBL :SCINTANA_1 Saccharomyces cerevisiae integrin analogue gene, complete eds., N = 1, Score = 404, p = 7. le-34

>TREMBL:HSGOLGIN_l product: "256 kD golgm"; H. sapiens mRNA for golgin Length = 2,185

HSPs:

Score = 411 (61.7 bits), Expect = 4.4e-34, P = 4.4e-34 Identities = 212/816 (25%), Positives = 420/816 (51%)

Query: 145 EMGNHNEN-TGEKLHLAQEQLALAGDKIASLERSLNLYRDKYQSSLSNIELLECQVKMLQ 203

+M + E+ G L +EQL ++ +ERSL+ YR KY ++ ++L+ + K LQ Sbjct: 119 DMDSEAEDLVGNSDSLNKEQLI QRLRRMERSLSSYRGKYSELVTAYQMLQREKKKLQ 175

Query: 204 GELGGIMGQEPENKGDHSKVRIYTSPCMIQEHQETQKRLSEVWQ-KVSQQDDLIQELRNK 262

G 1+ Q D S Rl +Q Q+ +K L E + + ++D I L+ + Sbjct: 176 G ILSQSQ DKSLRRIAELREELQMDQQAKKHLQEEFDASLEEKDQYISVLQTQ 227

Query: 263 LAC SNALVLEREKALIKLQADFASCTATHRYPPSSSEEC-ED—IKKILKHLQE 313

++ + + ++ K L +L+ A P S E ED K L+ LQ+ Sbjct: 228 VSLLKQRLRNGPMNVDVLKPLPQLEPQ-AEVFTKEENPESDGEPVVEDGTSVKTLETLQQ 286

Query: 314 QKDSQ CLH-VEEYQNLVKDLRVELEAVSEQKRNIMKDMMKLELDLHGLREETSA 366

+ Q C ++ ++ L E EA+ EQ ++++ K++ DLH + E+T Sbjct: 287 RVKRQENLLKRCKETIQSHKEQCTLLTSEKEALQEQLDERLQELEKIK-DLH-MAEKTKL 344

Query: 367 HIERKDKDITILQCRLQELQLEFTETQKLTLKKDKFLQEKDEMLQELEKKLTQV—QNSL 424

+ +D I Q Q+ + ET++ + + L+ K+E + +L ++ Q+ Q Sbjct: 345 ITQLRDAKNLIEQLE-QDKGMVIAETKR QMHETLEMKEEEIAQLRSRIKQMTTQGEE 400

Query: 425 LKKEKELEKQQCMATELEMTVKEAKQDKSKEAECKALQAEVQKLKNSLEEAKQQERLAAQ 484

L+++KE + ++ ELE + A+ K++EA K L+AE+ + ++E+ ++ER++ Q Sbjct: 401 LREQKE-KSERAAFEELEKALSTAQ—KTEEARRK-LKAEMDEQIKTIEKTSEEERISLQ 456

Query: 485 QA-AQCKEEAA-LAGCHLEDTQRKLQKGLLLDKQKADTIQELQRELQMLQKESSMAEKEQ 542

Q ++ K+E + E+ KLQK L +K+ A QEL ++LQ ++E E+ + Sbjct: 457 QELSRVKQEVVDVMKKSSEEQIAKLQK--LHEKELARKEQELTKKLQTRERE--FQEQMK 512

Query: 543 TSNRKRVEELSLELSEALRKLENSDKEKRQLQKT—VAEQDMKMNDMLDRIKHQHREQGS 600

+ K E L++S+ + E+ E+ +LQK + E + K+ D+ + Sbjct: 513 VALEKSQSEY-LKISQEKEQQESLALEELELQKKAILTESENKLRDLQQEAETYRTRILE 571

Query: 601 IKCKLEEDLQEATKLLED KREQLKKSKEHEKLMEG ELEALR-QEFKKKDKTL 651

++ LE+ LQE +D + E+ K +KE ++E ELE+L+ Q+ + L Sbjct: 572 LESSLEKSLQENKNQSKDLAVHLEAEKNKHNKEITVMVEKHKTELESLKHQQDALWTEKL 631

Query: 652 KENSRKLEEENENLRAELQCCSTQLESSL-NKYNTSQQVIQDLNKE IALQKESLMS 706

+ ++ + E E LR + C + E+ L +K Q I+++N++ + +++ L S Sbjct: 632 QVLKQQYQTEMEKLREK CEQEKETLLKDKEIIFQAHIEEMNEKTLEKLDVKQTELES 688

Query: 707 LQAQLDKALQKEKHYLQT—TITKEAYDALSRKSAACQDDLTQALEKLNHVTSETKSLQQ 764 L ++L + L K +H L+ ++ K+ D + ++ A D+ Q V S K + Sbjct: 689 LSSELSEVL-KARHKLEEELSVLKDQTDKMKQELEAKMDE—QKNHHQQQVDSIIKEHEV 745

Query: 765 SLTQTQEKKAQLEEEIIAYEERMKKLNTELRKLRGFHQESELEVHAFDKKLEEMSCQVLQ 824

S+ +T+ KA L+++I E +K+ + L++ + + E ++ + +L++ S ++ Sbjct: 746 SIQRTE--KA-LKDQINQLELLLKERDKHLKEHQAHVENLEADIKRSEGELQQASAKLDV 802

Query: 825 WQKQHQNDLKMLAAKEEQLREFQEEMAALKENLLEDDKEPCCLPQW SVPKDTC-R 878

+Q +Q+ A EQ + ++E++A L++ LL+ + E L + + KD C Sbjct: 803 FQS-YQS ATHEQTKAYEEQLAQLQQKLLDLETERILLTKQVAEVEAQKKDVCTE 855

Query: 879 LYRGNDQIMTNLEQWAKQQKVANEKLGNQLREQVNYIAKLS-GEKDHLHSVMVHLQQENK 937

L Q+ ++Q KQ +K+ + QV Y +KL G K+ + + +++EN Sbjct: 856 LDAHKIQVQDLMQQLEKQNSEMEQKVKSLT—QV-YESKLEDGNKEQEQTKQILVEKENM 912

Query: 938 KLK-KEIEEKKMKAENTRLCTK 958

L+ +E ++K+++ +L K Sbjct: 913 ILQMREGQKKEIEILTQKLSAK 934

Score = 338 (50.7 bits), Expect = 3. le-26, P = 3. le-26 Identities = 216/953 (22%), Positives = 468/953 (49%)

Query: 2 KDEAGERDRE—VSSLNS-KLL-SLQLDIKNLHDVCKRQRKTLQDN-QLCM EEAM 51

K+E E D E V Ξ K L +LQ +K ++ KR ++T+Q + + C +EA+ Sbjct: 260 KEENPEΞDGEPVVEDGTSVKTLETLQQRVKRQENLLKRCKETIQSHKEQCTLLTSEKEAL 319

Query: 52 NSSHDKKQAQALAFEESEVEFGSSKQCHLRQ LQQLK—KKLLVLQQELEFHTEELQ 105

D++ + ++ + + LR ++QL+ K +++ + + + H E L+ Sbjct: 320 QEQLDERLQELEKIKDLHMAEKTKLITQLRDAKNLIEQLEQDKGMVIAETKRQMH-ETLE 378

Query: 106 TSYYSLRQYQSILEKQTSDLVLLHHHCKLKEDEVILYEEEMGNHNENTGEKLHLAQEQL- 164

+ Q +S +++ T+ L K K + E E +T +K A+ +L Sbjct: 379 MKEEEIAQLRSRIKQMTTQGEELREQ-KEKSERAAFEELEKAL STAQKTEEARRKLK 434

Query: 165 ALAGDKIASLERSLNLYRDKYQSSLSNI—ELLECQVKMLQGELGGIMGQEPENKGDHSK 222

A ++I ++E++ R Q LS + E+++ K + ++ + Q+ K K Sbjct: 435 AEMDEQIKTIEKTSEEERISLQQELSRVKQEVVDVMKKSSEEQIAKL--QKLHEKELARK 492

Query: 223 VRIYTSPCMIQEHQETQKRLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQA 282

+ T +E +E Q+++ +K SQ + L ++ + +L LE ++LQ Sbjct: 493 EQELTKKLQTRE-REFQEQMKVALEK-SQΞEYL—KISQEKEQQESLALEE LELQK 544

Query: 283 DFASCTATHRYPPSΞSEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAV-SE 341

A T + +E E + + L+ + ++E +N KDL V LEA ++ Sbjct: 545 K-AILTEΞENKLRDLQQEAETYRTRILELESSLEKS LQENKNQSKDLAVHLEAEKNK 600

Query: 342 QKRNIMKDMMKLELDLHGLREETSAHIERKDKDITI-LQCRLQELQLEFTETQKLTLKKD 400

+ 1 + K + +L L+ + A K + + Q +++L+ E E +K TL KD Sbjct: 601 HNKEITVMVEKHKTELESLKHQQDALWTEKLQVLKQQYQTEMEKLR-EKCEQEKETLLKD 659

Query: 401 K FLQEKDEM-LQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTVKEAKQDKS 453

K ++E +E L++L+ K T+++ SL + E+ K + E E++V + + DK Sbjct: 660 KEIIFQAHIEEMNEKTLEKLDVKQTELE-SLSSELSEVLKARHKLEE-ELSVLKDQTDKM 717

Query: 454 K-EAECKALQAEVQKLKNSLEEAKQQERLAAQQAAQC-KEEAALAGCHLEDTQRKLQKGL 511

K E E K + + + ++ ++ ++ Q+ + K++ L++ + L++ Sbjct: 718 KQELEAK-MDEQKNHHQQQVDSIIKEHEVSIQRTEKALKDQINQLELLLKERDKHLKEHQ 776

Query: 512 L-LDKQKADTIQELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLENSDKEK 570

++ +AD 1+ + ELQ + + + Q++ ++ + +L++ +KL + + E+ Sbjct: 777 AHVENLEAD-IKRSEGELQQASAKLDVFQΞYQSATHEQTKAYEEQLAQLQQKLLDLETER 835

Query: 571 RQLQKTVAEQDMKMNDM LD--RIKHQHREQGSIK—CKLEEDLQEATKLLEDKREQL 623

L K VAE + + D+ LD +1+ Q Q K ++E+ ++ T++ E K E Sbjct: 836 ILLTKQVAEVEAQKKDVCTELDAHKIQVQDLMQQLEKQNSEMEQKVKΞLTQVYESKLEDG 895

Query: 624 KKSKEHEK—LMEGELEALRQEFKKKDKTLKENSRKLEEENENLRAELQCCSTQLESSLN 681

K +E K L+E E L+ +K K ++ ++KL + +++ + T+ ++ Sbjct: 896 NKEQEQTKQILVEKENMILQMREGQK-KEIEILTQKLSAKEDSIHILNEEYETKFKNQEK 954

Query: 682 KYNTSQQVIQDLNKEIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSRKSAAC 741

K +Q +++ + + K+ L+ +A+L K L E L+ + ++ ++A + A Sbjct: 955 KMEKVKQKAKEMQETL KKKLLDQEAKLKKEL--ENTALELSQKEKQFNAKMLEMAQA 1009

Query: 742 QD-DLTQALEKLNHVTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKLNTELRKLRGF 800

++ A+ +L T++ + ++ SLT+ + +L + I +E KKLN + +L+ Sbjct: 1010 NΞAGISDAVSRLE—TNQKEQIE-SLTEVHRR—ELNDVISIWE KKLNQQAEELQEI 1061

Query: 801 HQESELEVHAFDKKLEEMSCQVLQW—QKQHQNDLKMLAAKEEQLREFQEEMAALKENLL 858

H E+++ ++++ E+ ++L + +K+ N ++ KEE +++ + L+E L Sb_jct: 1062 H EIQLQEKEQEVAELKQKILLFGCEKEEMNK-EITWLKEEGVKQ-DTTLNELQEQLK 1116 Query: 859 EDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQ—WAKQQKVANEKLGNQLREQVNYI- 915

+ L Q K L + + +L++ + ++Q V + L + + +V+ + Sbjct: 1117 QKSAHVNSLAQ-DETKLKAHLEKLEVDLNKSLKENTFLQEQLVELKMLAEEDKRKVSELT 1175

Query: 916 AKLSGEKDHLHSVMVHLQQENKKLK-KEIEEKKMKAE 951

+KL + S+ ++ NK L+ K +E KK+ E Sbjct: 1176 SKLKTTDEEFQSLKSSHEKSNKSLEDKSLEFKKLSEE 1212

Score = 337 (50.6 bits), Expect = 4.0e-26, P = 4.0e-26 Identities = 215/951 (22%), Positives = 433/951 (45%)

Query: 10 REVSSLNSKLLSLQLDIKNLHDVCKRQRKTLQDNQLCMEEAMNSSHDKKQAQALAFEESE 69

+E + +++L L+ ++ K Q K L + EA + H+K+ + E+ + Sbjct: 560 QEAETYRTRILELESSLEKSLQENKNQSKDLAVHL EAEKNKHNKEIT—VMVEKHK 613

Query: 70 VEFGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYSLRQYQSILEKQTSDLVLLH 129

E S K H +Q +KL VL+Q+ + E+L+ Q + L K +++ Sbjct: 614 TELESLK—H-QQDALWTEKLQVLKQQYQTEMEKLREK CEQEKETLLKD-KEIIFQA 666

Query: 130 HHCKLKE DEVILYEEEMGNHNENTGEKL HLAQEQLALAGDKIASLERSLNLYRD 183

H ++ E +++ + + E+ + + E L H +E+L++ D+ +++ L D Sbjct: 667 HIEEMNEKTLEKLDVKQTELESLSSELSEVLKARHKLEEELSVLKDQTDKMKQELEAKMD 726

Query: 184 K YQSSLSNIELLECQVKMLQGE—LGGIMGQEPENKGDHSKVRIYTSPCMIQEHQE 237

+ +Q + +I + E +V + + E L + Q + K + ++ + Sbjct: 727 EQKNHHQQQVDSI-IKEHEVSIQRTEKALKDQINQLELLLKERDK-HLKEHQAHVENLEA 784

Query: 238 TQKRLΞEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQADFASCTATHRYPPSS 297

KR Q+ S + D+ Q ++ ++ E+ L +LQ T R Sbjct: 785 DIKRSEGELQQASAKLDVFQSYQS ATHEQTKAYEEQLAQLQQKLLDLE-TERIL 837

Query: 298 SEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAVSEQKRNIMKDMMKL-ELD 356

+ K + ++ QK C ++ ++ V+DL +LE + + +K + ++ E Sbjct: 838 LTKQVAEVEAQKKDVCTELDAHKIQVQDLMQQLEKQNSEMEQKVKSLTQVYESK 891

Query: 357 LH-GLREETSAHIERKDKDITILQCRL-QELQLEFTETQKLTLKKDKF—LQEKDEM-LQ 411

L G +E+ +K+ ILQ R Q+ ++E TQKL+ K+D L E+ E + Sbjct: 892 LEDGNKEQEQTKQILVEKENMILQMREGQKKEIEIL-TQKLSAKEDSIHILNEEYETKFK 950

Query: 412 ELEKKLTQVQNSLLK KEKELEKQQCMATELEMTVKEAKQDKSKEAECKALQAEVQ 466

EKK+ +V+ + K+K L+++ + ELE T E Q K K+ K L+ Q Sbjct: 951 NQEKKMEKVKQKAKEMQETLKKKLLDQEAKLKKELENTALELSQ-KEKQFNAKMLEM-AQ 1008

Query: 467 KLKNSLEEAKQQERLAAQQAAQCKEEAALAGCHLEDTQRKLQKGLLLDKQKADTIQELQR 526

+ +A RL Q Q + + L D +K L Q+A+ +QE+ Sbjct: 1009 ANSAGISDAVS—RLETNQKEQIESLTEVHRRELNDVISIWEKKL NQQAEELQEIH- 1062

Query: 527 ELQMLQKESSMAEKEQT SNRKRV EELSLELSEALRKLENSDKEKRQLQ 574

E+Q+ +KE +AE +Q K + +E ++ L +L+ K+K

Sbjct: 1063 EIQLQEKEQEVAELKQKILLFGCEKEEMNKEITWLKEEGVKQDTTLNELQEQLKQKSAHV 1122

Query: 575 KTVAEQDMKMNDMLDRIKHQHREQGSIKCKLEEDLQEATKLLEDKREQLKKSKEHEKLME 634

++A+ + K+ L++++ + L+E L E L E+ + ++ + K + Sbjct: 1123 NSLAQDETKLKAHLEKLEVDLNKSLKENTFLQEQLVELKMLAEEDKRKVSELTSKLKTTD 1182

Query: 635 GELEALRQEFKKKDKTLKENSRKLEEENENLRAELQCCSTQLESSLNKYNTSQQVIQDLN 694

E ++L+ +K +K+L++ S + ++ +E L +L C + E+ L T++ + + Sbjct: 1183 EEFQSLKSSHEKSNKSLEDKSLEFKKLSEELAIQLDICCKKTEALLEA-KTNELINISSS 1241

Query: 695 KEIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSRKSAACQDDLT QALE 750

K A+ + Q + K KE ++T E +A R+ Q+ L QA Sbjct: 1242 KTNAILSR-ISHCQHRTTKV—KEALLIKTCTVSEL-EAQLRQLTEEQNTLNISFQQATH 1297

Query: 751 KLNHVTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKLN TELRK—LRGFHQESE 805

+L ++ KS++ + +K L++E ++ + T+L+K + + Sbjct: 1298 QLEEKENQIKSMKADIESLVTEKEALQKEGGNQQQAASEKESCITQLKKELSENINAVTL 1357

Query: 806 LEVHAFDKKLE—EMΞCQVLQWQKQHQNDLKMLAAKEEQLREFQEEMAALKENLLEDDKE 863

++ +KK+E +S Q+ Q QN + L+ KE + +++ K LL D + Sbjct: 1358 MKEELKEKKVEISSLSKQLTDLNVQLQNSIS-LSEKEAAISΞLRKQYDEEKCELL-DQVQ 1415

Query: 864 PCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVANEKLGNQLRE QVNYIAKLSG 920

++ K+ D +W K+ + + N ++E Q+ +K + Sbjct: 1416 DLSFKVDTLΞKEKISALEQVDDWSNKFSEWKKKAQSRFTQHQNTVKELQIQLELKSKEAY 1475

Query: 921 EKDH-LHSVMVHLQQENKK LKKEIEEKKMKAE 951

EKD ++ + L Q+NK+ LK E+E+ K K E Sbjct: 1476 EKDEQINLLKEELDQQNKRFDCLKGEMEDDKSKME 1510

Score = 332 (49.8 bits), Expect = 1.4e-25, P = 1.4e-25 Identities = 209/953 (21%), Positives = 438/953 (45%) Query: 1 MKDEAGERDREVSSLNSKLLSLQLDIKNLHDVCKRQRKTLQDNQLCMEEAMNS SHD 56

MK + E+ ++ L+ K L+ + + + + R+R+ + ++ +E++ + S +

Sbjct: 470 MKKSSEEQIAKLQKLHEKELARK-EQELTKKLQTREREFQEQMKVALEKSQSEYLKISQE 528

Query: 57 KKQAQALAFEESEVEFGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYSLRQYQS 116 K+Q ++LA EE E++ K+ L + + KL LQQE E + + SL +

Sbjct: 529 KEQQESLALEELELQ KKAILTESEN KLRDLQQEAETYRTRILELESSLEKSLQ 581

Query: 117 ILEKQTSDLVLLHHHCKLKEDE—VILYEE EMGNHNENT—GEKLHLAQEQLALA 167

+ Q+ DL + K K ++ ++ E+ E H ++ EKL + ++Q

Sbjct: 582 ENKNQSKDLAVHLEAEKNKHNKEITVMVEKHKTELESLKHQQDALWTEKLQVLKQQYQTE 641

Query: 168 GDKIASL—ERSLNLYRDK YQSSLS—NIELLECQVKMLQGELGGIMGQEPENKGDH 220

+K+ + L +DK +Q+ + N + LE ++ + Q EL + + E

Sbjct: 642 MEKLREKCEQEKETLLKDKEIIFQAHIEEMNEKTLE-KLDVKQTELESLSSELSEVLKAR 700

Query: 221 SKVRIYTSPCMIQEHQETQKRLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKL 280

K+ S ++++ +T K E+ K+ +Q + Q+ + + + + ++R + +K

Sbjct: 701 HKLEEELS—VLKD--QTDKMKQELEAKMDEQKNHHQQQVDSIIKEHEVΞIQRTEKALKD 756

Query: 281 QADFASCTATHR—YPPSSSEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEA 338 Q + R + E+++ +K + + ++ +Q+ + +A

Sbjct: 757 QINQLELLLKERDKHLKEHQAHVENLEADIKRSEGELQQASAKLDVFQSYQSATHEQTKA 816

Query: 339 VSEQKRNIMKDMMKLELDLHGLREETSAHIERKDKDITILQCRLQELQLEFTETQKLTLK 398

EQ + + ++ LE + L ++ A +E + KD+ C EL + Q L +

Sbjct: 817 YEEQLAQLQQKLLDLETERILLTKQV-AEVEAQKKDV CT—ELDAHKIQVQDLMQQ 869

Query: 399 KDKFLQEKDEMLQELEKKLTQVQNΞLLKK-EKELEKQQCMATELEMTVKEAKQDKSKEAE 457

+K + EM Q++ K LTQV S L+ KE E+ + + E E + + ++ + KE E

Sbjct: 870 LEK QNSEMEQKV-KSLTQVYESKLEDGNKEQEQTKQILVEKENMILQMREGQKKEIE 925

Query: 458 C—KALQAEVQKLKNSLEEAKQQERLAAQQAAQCKEEAALAGCHLEDTQRK—LQKGLLL 513

+ L A+ + EE + + + ++ + K++A +++T +K L + L

Sbjct: 926 ILTQKLSAKEDΞIHILNEEYETKFKNQEKKMEKVKQKAK EMQETLKKKLLDQEAKL 981

Query: 514 DKQKADTIQEL-QRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLENSDKEKRQ 572

K+ +T EL Q+E Q K MA+ V L E + L ++ +R+

Sbjct: 982 KKELENTALELSQKEKQFNAKMLEMAQANSAGISDAVSRLETNQKEQIESL—TEVHRRE 1039

Query: 573 LQKTVAEQDMKMNDMLDRIKHQHREQGSIKCKLEEDLQEATKLLEDKREQLKKS KE 628

L ++ + K+N + ++ H Q K + +L++ L ++E++ K KE

Sbjct: 1040 LNDVISIWEKKLNQQAEELQEIHEIQLQEKEQEVAELKQKILLFGCEKEEMNKEITWLKE 1099

Query: 629 HEKLMEGELEALRQEFKKKDKTLKENSRKLEEENENLRAELQCCSTQLESSLNKYNTSQQ 688

+ L L+++ K+K + NS L ++ L+A L+ L SL + Q+

Sbjct: 1100 EGVKQDTTLNELQEQLKQKSAHV--NS--LAQDETKLKAHLEKLEVDLNKSLKENTFLQE 1155

Query: 689 VIQDLNKEIALQKESLMSLQAQL DKALQ—KEKHYLQTTITKEA YDALSRKSAA 740

+ +L K + L ++L D+ Q K H ++ + LS + A

Sbjct: 1156 QLVELKMLAEEDKRKVSELTSKLKTTDEEFQSLKSSHEKSNKSLEDKSLEFKKLSEE-LA 1214

Query: 741 CQDDL TQAL EKLNHVTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKL 790

Q D+ T+AL E +N +S+T ++ ++ Q + +++E ++ + +L

Sbjct: 1215 IQLDICCKKTEALLEAKTNELINISSSKTNAILSRISHCQHRTTKVKEALLIKTCTVSEL 1274

Query: 791 NTELRKLRGFHQESELEVHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLREFQEEM 850

+LR+L + +LEE Q+ K + D++ L ++E L Q+E

Sbjct: 1275 EAQLRQLTEEQNTLNISFQQATHQLEEKENQI KSMKADIESLVTEKEAL QKEG 1327

Query: 851 AALKENLLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVANEKLGNQLRE 910

+ +KE C + Q + K+ N +T +++ K++KV L QL +

Sbjct: 1328 G—NQQQAASEKESC-ITQ—LKKELSE NINAVTLMKEELKEKKVEISSLSKQLTD 1378

Query: 911 QVNYIAKLSGEKDHLHSVMVHLQQENKKLKKEIEEKKMKAE 951

Q+ LS ++ + S+ +E +L ++++ K +

Sbjct: 1379 LNVQLQNSISLSEKEAAISSLRKQYDEEKCELLDQVQDLSFKVD 1422

Score = 329 (49.4 bits), Expect = 2.9e-25, P = 2.9e-25

Identities = 226/941 (24%), Positives = 444/941 (47%)

Query: 61 QALAFEESEVE—FGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYSLRQYQSIL 118 Q L E+ +++ S+ LR++ +L+++L + QQ + EE S QY S+L

Sbjct: 165 QMLQREKKKLQGILSQSQDKSLRRIAELREELQMDQQAKKHLQEEFDASLEEKDQYISVL 224

Query: 119 EKQTSDLVLLHHHCKLKEDEV ILYEEEMGNHNENT GEKL HLAQEQLALA 167

+ Q S L + + D + + + E+ EN GE + + + L

Sbjct: 225 QTQVSLLKQRLRNGPMNVDVLKPLPQLEPQAEVFTKEENPESDGEPVVEDGTSVKTLETL 284

Query: 168 GDKIASLERSLNLYRDKYQSSLSNIELLECQVKMLQGELGGIMGQEPENKGDHSKVRIYT 227 ++ E L ++ QS LL + + LQ +L + QE E D + +

Sbjct: 285 QQRVKRQENLLKRCKETIQSHKEQCTLLTSEKEALQEQLDERL-QELEKIKD LHMAE 340

Query: 228 SPCMIQEHQETQKRLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQADFASC 287

+1 + ++ + + ++ Q +1 E + ++ L ++ E+ + +L++

Sbjct: 341 KTKLITQLRDAKNLIEQLEQDKGM VIAETKRQM--HETLEMKEEE-IAQLRSRIKQM 394

Query: 288 TATH RYPPSSSEEC—EDIKKILKHLQEQKDSQCLHVEEYQNLVKDL RVE 335

T R SE E+++K L Q+ ++++ E +K + R+

Sbjct: 395 TTQGEELREQKEKSERAAFEELEKALSTAQKTEEARRKLKAEMDEQIKTIEKTSEEERIS 454

Query: 336 LEA-VSEQKRNIMKDMMKL—ELDLHGLREETSAHIERKDKDITILQCRLQELQLEFTET 392 L+ +S K+ ++ D+MK E + L++ + RK++++T +LQ + EF E

Sbjct: 455 LQQELSRVKQEVV-DVMKKSSEEQIAKLQKLHEKELARKEQELTK KLQTREREFQEQ 510

Query: 393 QKLTLKKDKFLQEKDEMLQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTVKEAKQDK 452

K+ L+K + E ++ QE E+ Q SL +E EL+K+ + TE E +++ +Q+

Sbjct: 511 MKVALEKSQ—SEYLKISQEKEQ QESLALEELELQKKAIL-TESENKLRDLQQE- 561

Query: 453 SKEAECKALQAEVQKLKNSLEEAKQQER LAAQQAAQCKEEAALAGCHLEDTQR-K 506

++ + L+ E L+ SL+E K Q + L A++ KE + H + + K

Sbjct: 562 AETYRTRILELE-SSLEKSLQENKNQSKDLAVHLEAEKNKHNKEITVMVEKHKTELESLK 620

Query: 507 LQKGLLLDKQKADTIQELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRK-LEN 565

Q+ L ++ Q+ Q E++ L +E EKE K + + E K LE

Sbjct: 621 HQQDALWTEKLQVLKQQYQTEMEKL-REKCEQEKETLLKDKEII-FQAHIEEMNEKTLEK 678

Query: 566 SDKEKRQLQKTVAEQDMKMNDMLDRIKHQHREQGSI-KCKLEEDLQEA-TKLLEDKR—E 621

D ++ +L+ +E ++++L + +H+ E+ S+ K + ++ QE K+ E K +

Sbjct: 679 LDVKQTELESLSSE LSEVL-KARHKLEEELSVLKDQTDKMKQELEAKMDEQKNHHQ 733

Query: 622 QLKKS—KEHEKLMEGELEALRQEFKKKDKTLKENSRKLEEEN ENLRAELQCCSTQL 676

Q S KEHE ++ +AL+ + + + LKE + L+E ENL A+++ +L

Sbjct: 734 QQVDSIIKEHEVSIQRTEKALKDQINQLELLLKERDKHLKEHQAHVENLEADIKRSEGEL 793

Query: 677 ESSLNKYNTSQQVIQDLNKEIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSR 736 + + K + Q +++ +E L LQ +L L+ E+ L TK+ + ++

Sbjct: 794 QQASAKLDVFQSYQSATHEQTKAYEEQLAQLQQKL-LDLETERILL TKQVAEVEAQ 848

Query: 737 KSAACQD DLTQALEKLNHVTSETKSLQQSLTQTQEKKAQ—LEEEIIAYEE 785

K C + DL Q LEK N SE + +SLTQ E K + +E+ +

Sbjct: 849 KKDVCTELDAHKIQVQDLMQQLEKQN SEMEQKVKSLTQVYESKLEDGNKEQEQTKQI 905

Query: 786 RMKKLNTELRKLRGFHQESELEVHAFDKKLEEMSCQVL—QWQKQHQNDLKMLAAKEEQL 843

++K N L+ G Q+ E+E+ +E S +L +++ + +N K + +++

Sbjct: 906 LVEKENMILQMREG—QKKEIEILTQKLSAKEDSIHILNEEYETKFKNQEKKMEKVKQKA 963

Query: 844 REFQEEMAALKENLLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKV 899

+E QE LK+ LL+ + + L + + L + Q + + A+

Sbjct: 964 KEMQE TLKKKLLDQEAK LKK-ELENTALELSQKEKQFNAKMLEMAQANSAGIΞD 1016

Query: 900 ANEKLGNQLREQVNYIAKLSG-EKDHLHSVMVH-LQQENKKLKK—EIEEKKMKAENTRL 955 A +L +EQ+ + ++ E + + S+ L Q+ ++L++ EI+ ++ + E L

Sbjct: 1017 AVSRLETNQKEQIESLTEVHRRELNDVISIWEKKLNQQAEELQEIHEIQLQEKEQEVAEL 1076

Query: 956 CTKALGPSRTESTQREKVCGTLGWKGLPQD 985

K L E + K L +G+ QD

Sbjct: 1077 KQKIL-LFGCEKEEMNKEITWLKEEGVKQD 1105

Score = 326 (48.9 bits), Expect = 6.0e-25, P = 6.0e-25

Identi ties = 220/907 (24%), Positives = 444/907 (48%)

Query: 67 ESEVEFGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYSLRQYQSILE KQTS 123

E+E G+S + QL Q +++ EL T+Y L++ + L+ Q+

Sbjct: 123 EAEDLVGNΞDSLNKEQLIQRLRRMERSLSSYRGKYSELVTAYQMLQREKKKLQGILSQSQ 182

Query: 124 DLVLLHHHCKLKEDEVILYEEEMGNHNENTGEKLHLAQEQLALAGDKIASLERSLNLYRD 183 D L +L+E+ + +++ H + E+ + E+ 1+ L+ ++L +

Sbjct: 183 DKSL-RRIAELREE--LQMDQQAKKHLQ EEFDASLEE KDQYISVLQTQVSLLKQ 233

Query: 184 KYQSSLSNIELLECQVKMLQGELGGIMGQE-PENKG DHSKVR-IYTSPCMIQEHQ 236

+ ++ N+++L+ + L+ + +E PE+ G D + V+ + T ++ +

Sbjct: 234 RLRNGPMNVDVLK-PLPQLEPQAEVFTKEENPEΞDGEPVVEDGTΞVKTLETLQQRVKRQE 292

Query: 237 ETQKRLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQADFASCTATHRYPPS 296

KR E Q +Q L+ K A L ER + L K++ D T

Sbjct: 293 NLLKRCKETIQSHKEQCTLLTS—EKEALQEQLD-ERLQELEKIK-DLHMAEKTKLIT— 346

Query: 297 SSEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAVSEQKRNIMKDMMKLELD 356

+ D K +++ L++ K + E + + + L ++ E ++ Q R+ +K M +

Sbjct: 347 QLRDAKNLIEQLEQDKGM— IAETKRQMHETLEMKEEEIA-QLRSRIKQMTTQGEE 400 Query: 357 LHGLREETS-AHIERKDKDITILQCRLQE LQLEFTETQKLTLKKDKFLQEKDEMLQ 411

L +E++ A E +K ++ Q + +E L+ E E K T++K +E+ + Q Sbjct: 401 LREQKEKSERAAFEELEKALSTAQ-KTEEARRKLKAEMDEQIK-TIEKTSE-EERISLQQ 457

Query: 412 ELEKKLTQVQNSLLKK-EKELEKQQCMATELEMTVKEAKQDKSKEAECKALQAEVQKLKN 470

EL + +V + + K E+++ K Q + E E+ KE Q+ +K+ + + + + Q +K Sbjct: 458 ELSRVKQEVVDVMKKSSEEQIAKLQKLH-EKELARKE--QELTKKLQTREREFQEQ-MKV 513

Query: 471 SLEEAKQQERLAAQQAAQCKEEAALAGCHLEDTQRKLQ-KGLLLD-KQKADTIQELQREL 528

+LE++ Q E L Q + +E AL L+ + + L D +Q+A+T + EL Sbjct: 514 ALEKS-QSEYLKISQEKEQQESLALEELELQKKAILTESENKLRDLQQEAETYRTRILEL 572

Query: 529 QMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLENS-DKEKRQLQKTVAEQDMKMNDM 587

+ E S+ E + S V L E ++ +++ +K K +L+ +QD + Sbjct: 573 ES-SLEKSLQENKNQSKDLAVH-LEAEKNKHNKEITVMVEKHKTELESLKHQQDALWTEK 630

Query: 588 LDRIKHQHR-EQGSIKCKLEEDLQEATKLLEDKRE--QLKKSKEHEKLMEGELEALRQEF 644

L +K Q++ E ++ K E QE LL+DK Q + +EK +E +L+ + E Sbjct: 631 LQVLKQQYQTEMEKLREKCE QEKETLLKDKEIIFQAHIEEMNEKTLE-KLDVKQTEL 686

Query: 645 KKKDKTLKE— SR-KLEEENENLRAELQCCSTQLESSLNKY-NTSQQVIQDLNKE—IA 698

+ L E +R KLEEE L+ + +LE+ +++ N QQ + + KE ++ Sbjct: 687 ESLSSELSEVLKARHKLEEELSVLKDQTDKMKQELEAKMDEQKNHHQQQVDSIIKEHEVS 746

Query: 699 LQK-ESLMSLQA-QLDKAL-QKEKHYLQTTITKEAYDALSRKΞ AACQDDLTQAL 749

+Q+ E + Q QL+ L +++KH + E +A ++S A+ + D+ Q+ Sbjct: 747 IQRTEKALKDQINQLELLLKERDKHLKEHQAHVENLEADIKRSEGELQQASAKLDVFQSY 806

Query: 750 EKLNHVTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKLNTELRKLRGFHQEΞELEVH 809

+ H +TK+ ++ L Q Q+K LE E I +++ ++ + + + +++V Sbjct: 807 QSATH—EQTKAYEEQLAQLQQKLLDLETERILLTKQVAEVEAQKKDVCTELDAHKIQVQ 864

Query: 810 AFDKKLEEMSCQVLQWQKQHQN—DLKMLAAKEEQLREFQEEMAALKENLL EDDKE 863

++LE+ + ++ Q K + K+ +EQ E +++ KEN++ E K+ Sbjct: 865 DLMQQLEKQNSEMEQKVKSLTQVYEΞKLEDGNKEQ—EQTKQILVEKENMILQMREGQKK 922

Query: 864 PC-CLPQ-WSVPKDTCRLYRGNDQIMTNLE-QWAKQQKVANE—KLGNQLREQV-NYIAK 917

L Q S +D+ + N++ T + Q K +KV + ++ L++++ + AK Sbjct: 923 EIEILTQKLSAKEDSIHIL—NEEYETKFKNQEKKMEKVKQKAKEMQETLKKKLLDQEAK 980

Query: 918 LSGEKDHLHSVMVHLQQENKKLKKEIEEKKMKAENTRLCTKALGPSRTESTQREKV 973

L K L + + L Q+ K+ ++ E M N+ + A+ SR E+ Q+E++ Sbjct: 981 L KKELENTALELSQKEKQFNAKMLE—MAQANSAGISDAV—SRLETNQKEQI 1029

Score = 318 (47.7 bits). Expect = 4.4e-24, P = 4.4e-24 Identities = 184/827 (22%), Positives = 405/827 (48%)

Query: 1 MKDEAGERDREVSSLNSKLLSLQLDIKNLHDVCKRQRKTLQDNQLCMEEAMNSSHDKK-Q 59

++ E G + + S S + L+ ++ + ++ L++ ++ + D Q Sbjct: 1323 LQKEGGNQQQAASEKESCITQLKKELSENINAVTLMKEELKEKKVEISSLSKQLTDLNVQ 1382

Query: 60 AQ-ALAFEESEVEFGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYS-LRQYQS- 116

Q +++ E E S + +Q + K +LL Q+L F + L Ξ L Q Sbjct: 1383 LQNSISLSEKEAAISSLR KQYDEEKCELLDQVQDLSFKVDTLSKEKISALEQVDDW 1438

Query: 117 ILE-KQTSDLVLLHHHCKLKEDEVILYEEEMGNHNENTGEKLHLAQEQLALAGDKIA 172

E K+ + H +KE ++ L + + ++ E+++L +E+L + Sbjct: 1439 SNKFSEWKKKAQSRFTQHQNTVKELQIQLELKSKEAYEKD—EQINLLKEELDQQNKRFD 1496

Query: 173 SLERSLNLYRDKYQSSLSNIEL-LECQVKMLQGELGGIMGQEP-ENKGDHSKVRIYTSPC 230

L+ + + K + SN+E L+ Q + EL + Q+ E + + ++ Y Sbjct: 1497 CLKGEMEDDKSKMEKKESNLETELKSQTARIM-ELEDHITQKTIEIESLNEVLKNYNQQK 1555

Query: 231 MIQEHQETQKRLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQADFASCTAT 290

I EH+E ++L + ++D+ ++E K+ L LE + +K + + Sbjct: 1556 DI-EHKELVQKLQHFQELGEEKDNRVKEAEEKI LTLENQVYSMKAELETKKKELE 1609

Query: 291 HRYPPSSSEECEDIKKILKHLQEQKDSQCLHVE-EYQNLVKDLRVELEAVSEQKRNIMKD 349

H S+E E++K + L+ + ++ ++ + + + ++ +L + E+K ++ Sbjct: 1610 HVNLSVKΞKE-EELKALEDRLESESAAKLAELKRKAEQKIAAIKKQLLSQMEEK EE 1664

Query: 350 MMKLELDLHGLREETSAHIERKDKDITILQCRLQELQLEFTETQKL--TLKKDKFLQEKD 407

K + H E + ++ +++++ IL+ +L+ ++ +ET + + K E++ Sbjct: 1665 QYKKGTESH—LSELNTKLQEREREVHILEEKLKSVESSQSETLIVPRSAKNVAAYTEQE 1722

Query: 408 EM LQEL-EKKLTQVQNSLLKKEKEL EKQQCMATELEMTVK-EAKQDKSKE 455

E +Q+ E+K++ +Q +L +KEK L EK++ +++ EM + + + K + Sbjct: 1723 EADSQGCVQKTYEEKISVLQRNLTEKEKLLQRVGQEKEETVSSHFEMRCQYQERLIKLEH 1782

Query: 456 AECKAL—QAEVQKLKNSLEEAKQQERLAAQQAAQCK—EEAALAGCHLEDTQRKLQKGL 511 AE K Q+ + L+ LEE ++ L Q + + + A +LE+ +QK L Sbjct: 1783 AEAKQHEDQSMIGHLQEELEEKNKKYSLIVAQHVEKEGGKNNIQAKQNLENVFDDVQKTL 1842

Query: 512 LLDKQKADTIQELQRELQMLQKESSMAEKEQTSNRKRVEELS--LELSEALRKLENSDKE 569

++K T Q L+++++ L +S + +++ +R +EEL+ E +AL++++ +K Sbjct: 1843 QEKELTCQILEQKIKEL—DSCLVRQKEV-HRVEMEELTSKYEKLQALQQMDGRNKP 1896

Query: 570 KRQLQKTVAEQD MKMNDMLDRIKHQHREQGSIKCKLEEDLQEATKLLEDKREQLKK- 625

L++ E+ + +L ++ QH + E + Q+ K + ++ L+ Sbjct: 1897 TELLEENTEEKSKSHLVQPKLLSNMEAQHNDLEFKLAGAEREKQKLGKEIVRLQKDLRML 1956

Query: 626 SKEHEKLMEGELEALRQEFKKKDKTLKENSRKLEEENENLRAELQCCSTQLESSLNKYNT 685

KEH++ ELE L++E+ + E K+++E E+L EL+ ST L+ + ++NT Sbjct: 1957 RKEHQQ ELEILKKEYDQ EREEKIKQEQEDL--ELKHNST-LKQLMREFNT 2003

Query: 686 S-QQVIQDLNKEIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSRKSAACQDD 744

Q Q+L I ++A+L ++ Q+E + L I E D L R +A ++

Sbjct: 2004 QLAQKEQELEMTIKETINKAQEVEAELLESHQEETNQLLKKIA-EKDDDLKR-TAKRYEE 2061

Query: 745 LTQALEKLNHVTSETKSLQQSLTQTQEKKAQ-LEEEIIAYEERMK—KLNTELRKLRGFH 801

+ A E+ +T++ + LQ L + Q+K Q LE+E + + +L T+L + Sbjct: 2062 ILDAREE—EMTAKVRDLQTQLEELQKKYQQKLEQEENPGNDNVTIMELQTQLAQKTTLI 2119

Query: 802 QESELEVHAFDKKLEEMSCQVLQWQK 827

+S+L+ F +++ + ++ +++K Sbjct: 2120 SDSKLKEQEFREQIHNLEDRLKKYEK 2145

Score = 316 (47.4 bits), Expect = 7.1e-24, P = 7.1e-24 Identities = 213/977 (21%), Positives = 454/977 (46%)

Query: 4 EAGERD-REVSSLNSKLLΞLQLD-IKNLHDVCKRQRKTLQDNQLCMEEAMNSSHDKKQAQ 61

E R+ +V S+ K L+ Q + ++ +H++ + Q K + +L + + ++ + Sbjct: 1034 EVHRRELNDVISIWEKKLNQQAEELQEIHEI-QLQEKEQEVAELKQKILLFGCEKEEMNK 1092

Query: 62 ALAFEESEVEFGSSKQCHLRQLQ-QLKKKLL VLQQE--LEFHTEELQTSYYSLRQY 114

+ + + E G + L +LQ QLK+K + Q E L+ H E+L+ + Sbjct: 1093 EITWLKEE GVKQDTTLNELQEQLKQKSAHVNSLAQDETKLKAHLEKLEVDLNKSLKE 1149

Query: 115 QSILEKQTΞDLVLLHHHCKLKEDEV ILYEEEMGNHNENTGEKLHLAQEQLALAGDKI 171

+ L++Q +L +L K K E+ + +E +++ EK + + E +L K+ Sbjct: 1150 NTFLQEQLVELKMLAEEDKRKVSELTSKLKTTDEEFQSLKSSHEKΞNKSLEDKSLEFKKL 1209

Query: 172 AS-LERSLNLYRDKYQSSLS—NIELLECQVKMLQGELGGIMGQEPENKGDHSKVRIYTS 228

+ L L++ K ++ L EL+ L I +++ K +

Sbjct: 1210 SEELAIQLDICCKKTEALLEAKTNELINISSSKTNAILSRI—SHCQHRTTKVKEALLIK 1267

Query: 229 PCMIQEHQ ETQKRLSEVWQKVSQQ-DDLIQELRNKLACSNALVLEREKALIKL 280

C + E + E Q L+ +Q+ + Q ++ ++++ A +LV E+E L Sbjct: 1268 TCTVSELEAQLRQLTEEQNTLNISFQQATHQLEEKENQIKSMKADIESLVTEKEA L 1323

Query: 281 QADFASCTATHRYPPSSSEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAVS 340

Q + + + S E C I ++ K L E ++ L EE +K+ +VE+ ++S Sbjct: 1324 QKEGGN QQQAASEKESC—ITQLKKELSENINAVTLMKEE LKEKKVEISSLS 1373

Query: 341 EQKRNIMKDMMKLELDLHGLREETSAHIERKDKDITILQCRLQEL--QLEFTETQKLT-L 397

+Q ++ + + L S+ ++ D++ L ++Q+L +++ +K++ L Sbjct: 1374 KQLTDLNVQLQN-SISLSEKEAAISΞLRKQYDEEKCELLDQVQDLSFKVDTLSKEKISAL 1432

Query: 398 KK-DKFLQEKDEMLQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTV KEAKQDKΞ 453

++ D + + E ++ + + TQ QN++ + + +LE + A E + + KE ++ Sbjct: 1433 EQVDDWSNKFSEWKKKAQSRFTQHQNTVKELQIQLELKSKEAYEKDEQINLLKEELDQQN 1492

Query: 454 KEAECKALQAEVQKLKNSLEEAKQQERLAAQQAAQCKEEAALAGCHLE-DTQRKLQKGLL 512

K +C + E K K +E+ + L +Q A + E + +E ++ ++ K Sbjct: 1493 KRFDCLKGEMEDDKSKMEKKESNLETELKSQTARIMELEDHITQKTIEIESLNEVLKNY- 1551

Query: 513 LDKQKADTIQELQRELQMLQKESSMAEKEQTSNRKRVEELΞLELSEALRKLENSDKEKRQ 572

++QK +EL ++LQ Q+ + +++ L ++ +LE KE Sbjct: 1552 -NQQKDIEHKELVQKLQHFQELGEEKDNRVKEAEEKILTLENQVYSMKAELETKKKELEH 1610

Query: 573 LQKTVAEQDMKMNDMLDRIKHQHREQ-GSIKCKLEEDLQEATKLL EDKREQLKKSK 627

+ +V ++ ++ + DR++ + + +K K E+ + K L E+K EQ KK Sbjct: 1611 VNLSVKΞKEEELKALEDRLESESAAKLAELKRKAEQKIAAIKKQLLSQMEEKEEQYKKGT 1670

Query: 628 EHEKLMEGELEALRQEFKKKDKTLKENSRKLEE-ENENL RAELQCCSTQLESSLNK 682

E EL QE +++ L+E + +E ++E L A+ T+ E + ++ Sbjct: 1671 ESHL SELNTKLQEREREVHILEEKLKSVESSQΞETLIVPRSAKNVAAYTEQEEADSQ 1727

Query: 683 YNTSQQVIQDLNKEIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSRKSA 739

T ++ I L + + +KE L+ Q +K H+ +E L A Sbjct: 1728 GCVQKTYEEKISVLQRNLT-EKEKLLQRVGQ-EKEETVSSHFEMRCQYQERLIKLEHAEA 1785 Query: 740 ACQDDLTQALEKLNHVTSET--KSLQQSLTQTQEKKAQLEEEIIAYEERMKKLNTELRKL 797

+D Q++ + H+ E K+ + SL Q + + + I ++ ++ + +++K Sbjct: 1786 KQHED—QSM—IGHLQEELEEKNKKYSLIVAQHVEKEGGKNNIQAKQNLENVFDDVQKT 1841

Query: 798 RGFHQESELEVHAFDKKLEEM-SCQVLQWQKQHQNDLKMLAAKEEQLREFQEEMAALKEN 856

QE EL ++K++E+ SC V Q ++ H+ +++ L +K E+L+ Q+ K Sbjct: 1842 L QEKELTCQILEQKIKELDSCLVRQ-KEVHRVEMEELTSKYEKLQALQQMDGRNKPT 1897

Query: 857 -LLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVANEKLGNQLREQVNYI 915

LLE++ E PK + ++ + L A+++K +KLG ++ + Sbjct: 1898 ELLEENTEEKSKSHLVQPKLLSNMEAQHNDLEFKLAG-AEREK QKLGKEIVRLQKDL 1953

Query: 916 AKLΞGE-KDHLHSVMVHLQQENK-KLKKEIEEKKMKAENTRLCTKALGPSRTESTQREK 972

L E + L + QE + K+K+E E+ ++K +T + + T+ Q+E+ Sbjct: 1954 RMLRKEHQQELEILKKEYDQEREEKIKQEQEDLELKHNST—LKQLMREFNTQLAQKEQ 2010

Score = 301 (45.2 bits), Expect = 2.9e-22, P = 2.9e-22 Identities = 221/952 (23%), Positives = 441/952 (46%)

Query: 1 MKDEAGERDREVSSLNSKLLSLQLDIKNLHDVCKRQRKTLQDNQL CMEEAMNSSHD- 56

+K A E R+VS L SKL + + ++L ++ K+L+D L + E + D Sbjct: 1160 LKMLAEEDKRKVSELTSKLKTTDEEFQSLKSSHEKSNKSLEDKSLEFKKLSEELAIQLDI 1219

Query: 57 —KKQAQALAFEESE-VEFGSΞK-QCHLRQLQQLKKKLLVLQQELEFHT EELQTSYY 109

KK L + +E + SSK L ++ + + +++ L T EL+ Sbjct: 1220 CCKKTEALLEAKTNELINISSSKTNAILSRISHCQHRTTKVKEALLIKTCTVSELEAQLR 1279

Query: 110 SLRQYQSILEKQTSDLVLLHHHCKLKEDEVILYEEEMGNHNENTGEKLHLAQE QLAL 166

L + Q+ L H + KE+++ + ++ EK L +E Q Sbjct: 1280 QLTEEQNTLNISFQQAT HQLEEKENQIKSMKADI ESLVTEKEALQKEGGNQQQA 1333

Query: 167 AGDKIASLERSLNLYRDKYQSSLSNIELLECQVKMLQGELGGIMGQEPENKGDHSKVRIY 226

A +K E + + + +++ + L++ ++K + E+ + Q + V++ Sbjct: 1334 ASEK ESCITQLKKELSENINAVTLMKEELKEKKVEISSLSKQLTD LNVQLQ 1384

Query: 227 TSPCMIQEHQETQKRLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQADFAS 286

Ξ + ++ + ++ + D +Q+L K+ + L E+ AL ++ D+++ Sbjct: 1385 NSISLSEKEAAISSLRKQYDEEKCELLDQVQDLSFKV DTLSKEKISALEQVD-DWSN 1440

Query: 287 CTATHRYPPSS—SEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKD LRVE-LE 337

+ + S ++ +K++ L E K + +E NL+K+ R + L+ Sbjct: 1441 KFSEWKKKAQSRFTQHQNTVKELQIQL-ELKSKEAYEKDEQINLLKEELDQQNKRFDCLK 1499

Query: 338 AVSEQKRNIM-KDMMKLELDLHGLRE ETSAHIERKDKDITILQCRLQEL-QLEFTET 392

E ++ M K LE +L E HI +K +1 L L+ Q + E Sbjct: 1500 GEMEDDKSKMEKKESNLETELKSQTARIMELEDHITQKTIEIESLNEVLKNYNQQKDIEH 1559

Query: 393 QKLTLKKDKFLQ EKDEMLQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTVKEAK 449

++L K F + EKD ++E E+K+ ++N + + ELE ++ + ++VK Sbjct: 1560 KELVQKLQHFQELGEEKDNRVKEAEEKILTLENQVYSMKAELETKKKELEHVNLSVK 1616

Query: 450 QDKSKEAECKALQAEVQKLKNSLEEAKQQERLAAQQAAQCKEEAALAGCHLEDTQRKLQK 509

SKE E KAL+ ++ S + + +R A Q+ A K++ +E+ + + +K Sbjct: 1617 SKEEELKALEDRLES—ESAAKLAELKRKAEQKIAAIKKQLL SQMEEKEEQYKK 1668

Query: 510 GLLLDKQKADT-IQELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLENSDK 568

G + +T +QE +RE+ +L+++ E Q+ + S + A + E +D Sbjct: 1669 GTESHLSELNTKLQEREREVHILEEKLKSVESSQSETL—IVPRSAKNVAAYTEQEEADS 1726

Query: 569 E KRQLQK-TVAEQDMKMND-MLDRIKHQHREQGSIKCKLEEDLQEATKLLEDKREQ 622

+ K +K +V ++++ + +L R+ Q +E+ ++ E Q +L+ K E Sbjct: 1727 QGCVQKTYEEKISVLQRNLTEKEKLLQRVG-QEKEE-TVSΞHFEMRCQYQERLI—KLEH 1782

Query: 623 LKKSKEHE-KLMEGEL-EALRQEFKKKDKTLKENSRKLEEENENLRAELQCCSTQLESSL 680

+ +K+HE + M G L E L ++ KK + ++ K E N++A+ LE Sbjct: 1783 AE-AKQHEDQSMIGHLQEELEEKNKKYSLIVAQHVEK-EGGKNNIQAK QNLE 1832

Query: 681 NKYNTSQQVIQDLNKEIALQKESLMSLQAQLDKAL—QKEKHYLQTTITKEAYDALSR-K 737

N ++ Q+ +Q+ KE+ Q L +LD L QKE H ++ Y+ L + Sbjct: 1833 NVFDDVQKTLQE—KELTCQ—ILEQKIKELDSCLVRQKEVHRVEMEELTSKYEKLQALQ 1888

Query: 738 SAACQDDLTQALEKLNHVTSETKSLQQSLTQTQEKKAQ-LEEEIIAYEERMKKLNTEL— 794

++ T+ LE+ S++ +Q L E + LE ++ E +KL E+ Sbjct: 1889 QMDGRNKPTELLEENTEEKSKSHLVQPKLLSNMEAQHNDLEFKLAGAEREKQKLGKEIVR 1948

Query: 795 —RKLRGFHQESELEVHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLREFQEEMAA 852

+ LR +E + E+ K+ ++ + ++ Q+Q +LK + ++ +REF ++A Sbjct: 1949 LQKDLRMLRKEHQQELEILKKEYDQEREEKIK-QEQEDLELKHNSTLKQLMREFNTQLAQ 2007

Query: 853 LKENLLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVANEKLGNQLREQV 912 ++ L KE Q V + + Q TN Q K K+A EK + R Sbjct: 2008 KEQELEMTIKETINKAQ-EVEAELLESH QEETN—QLLK--KIA-EKDDDLKRTAK 2057

Query: 913 NYIAKLSGEKDHLHSVMVHLQQENKKLKKEIEEKKMKAEN 952

Y L ++ + + + LQ + ++L+K+ ++K + EN Sbjct: 2058 RYEEILDAREEEMTAKVRDLQTQLEELQKKYQQKLEQEEN 2097

Score = 300 (45.0 bits), Expect = 3.7e-22, P = 3.7e-22 Identities = 195/961 (20%), Positives = 435/961 (45%)

Query: 1 MKDEAGERDREVΞSLNSKLLSLQLDIKN—LHDVCKRQRKTLQDNQLCMEEAMNSSHDKK 58

+KD+ + +N K L +LD+K L + + L+ +EE ++ D+ Sbjct: 657 LKDKEIIFQAHIEEMNEKTLE-KLDVKQTELESLSSELSEVLKARHK-LEEELSVLKDQT 714

Query: 59 QAQALAFEESEVEFGSSKQCHLRQLQQLKKKLLV-LQQELEFHTEELQTΞYYSLRQYQSI 117

+E E + K H +Q+ + K+ V +Q+ + +++ L++ Sbjct: 715 DKMK QELEAKMDEQKNHHQQQVDSIIKEHEVSIQRTEKALKDQINQLELLLKERDKH 771

Query: 118 LEKQTSDLVLLHHHCKLKEDEVILYEEEMG NHNENTGEKLHLAQEQLALAGDKIASL 174

L++ + + L K E E+ ++ ++ T E+ +EQLA K+ L Sbjct: 772 LKEHQAHVENLEADIKRSEGELQQASAKLDVFQSYQSATHEQTKAYEEQLAQLQQKLLDL 831

Query: 175 ERSLNLYRDKYQSSLSNIELLECQVKMLQGELGGIMGQ-EPENKGDHSKVRIYTSPCMIQ 233

E L + + + + ++ + ++ +M Q E +N KV+ T Sbjct: 832 ETERILLTKQVAEVEAQKKDVCTELDAHKIQVQDLMQQLEKQNSEMEQKVKSLTQ-VYEΞ 890

Query: 234 EHQETQKRLSEVWQKVSQQDDLIQELRN KLACSNALVLEREKALIKLQADFASCTA 289

+ ++ K + Q + +++++I ++R ++ + +E ++ L ++ + Sbjct: 891 KLEDGNKEQEQTKQILVEKENMILQMREGQKKEIEILTQKLSAKEDSIHILNEEYET 947

Query: 290 THRYPPSSSEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAVSEQKRNIMKD 349

++ + ++ E +K+ K +QE + L E L K+L +S++++ Sbjct: 948 —KFK-NQEKKMEKVKQKAKEMQETLKKKLLDQEA—KLKKELENTALELSQKEKQFNAK 1002

Query: 350 MMKL-ELDLHGLREETSA-HIERKDKDITILQCRLQELQLEFTETQKLTLKKDKFLQEKD 407

M+++ + + G+ + S +K++ ++ + +EL + +K ++ + LQE Sbjct: 1003 MLEMAQANSAGIΞDAVΞRLETNQKEQIESLTEVHRRELNDVIΞIWEKKLNQQAEELQEIH 1062

Query: 408 EM-LQELEKKLTQVQNSLLK KEKELEKQQCMATE LEMTVKEAKQD-KSKEAEC 458

E+ LQE E+++ +++ +L +++E+ K+ E + T+ E ++ K K A Sbjct: 1063 EIQLQEKEQEVAELKQKILLFGCEKEEMNKEITWLKEEGVKQDTTLNELQEQLKQKSAHV 1122

Query: 459 KALQAEVQKLKNSLEEAKQQERLAAQQAAQCKEEAALAGCHLEDTQRKLQKGLLLDKQKA 518

+L + KLK LE+ + + ++ +E+ E+ +RK+ + L K K Sbjct: 1123 NSLAQDETKLKAHLEKLEVDLNKSLKENTFLQEQLVELKMLAEEDKRKVSE—LTSKLKT 1180

Query: 519 DTIQELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLENSDKEKRQLQKTVA 578

T +E Q +K + E + +K EEL+++L +K E + K + + Sbjct: 1181 -TDEEFQSLKSSHEKSNKSLEDKSLEFKKLSEELAIQLDICCKKTEALLEAKTN—ELIN 1237

Query: 579 EQDMKMNDMLDRIKH-QHREQGSIKCKLEEDLQEATKLLEDKREQLKKSKEHEKLMEGEL 637

K N +L Rl H QHR K++E L T + + QL++ E + + Sbjct: 1238 ISSSKTNAILSRISHCQHRTT KVKEALLIKTCTVSELEAQLRQLTEEQNTLNISF 1292

Query: 638 EALRQEFKKKD KTLKENSRKLEEENENLR AELQCCSTQLESSL 680

+ + ++K+ K++K + L E E L+ +E + C TQL+ L

Sbjct: 1293 QQATHQLEEKENQIKSMKADIESLVTEKEALQKEGGNQQQAASEKESCITQLKKELSENI 1352

Query: 681 NKYNTSQQVIQDLNKEIALQKESLMSLQAQLDKALQ-KEKHYLQTTITKEAYDALSRKSA 739

N ++ +++ EI+ + L L QL ++ EK +++ K+ YD + Sbjct: 1353 NAVTLMKEELKEKKVEISSLSKQLTDLNVQLQNSISLSEKEAAISSLRKQ-YDEEKCELL 1411

Query: 740 ACQDDLTQALEKLN-HVTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKLNTELR-KL 797

DL+ ++ L+ S + + + E K + + ++ +K+L +L K Sbjct: 1412 DQVQDLSFKVDTLSKEKISALEQVDDWSNKFSEWKKKAQSRFTQHQNTVKELQIQLELKS 1471

Query: 798 RGFHQESELEVHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLR-EFQEEMAALKEN 856

+ +++ E +++ ++L++ + + + + ++D + KE L E + + A + E Sbjct: 1472 KEAYEKDE-QINLLKEELDQQNKRFDCLKGEMEDDKSKMEKKEΞNLETELKΞQTARIME- 1529

Query: 857 LLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVANEKLGNQLREQVNYIA 916

LED + + T + N+ ++ N Q QK K +L +++ + Sbjct: 1530 -LEDH ITQKTIEIESLNE-VLKNYNQ QKDIEHK ELVQKLQHFQ 1570

Query: 917 KLSGEKDH LHSVMVHLQQENKKLKKEIEEKKMKAENTRLCTKA 959

+L EKD+ ++ L+ + +K E+E KK + E+ L K+ Sbjct: 1571 ELGEEKDNRVKEAEEKILTLENQVYSMKAELETKKKELEHVNLSVKS 1617

Score = 298 (44.7 bits), Expect = 6.1e-22, P = 6.1e-₂2 Identities = 207/886 (23%), Positives = 412/886 (46%) Query: 47 MEEAMNSSHDKKQAQALAFEESEVEFGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQT 106 + E N+ + Q EE E + S K ++ L + LQ+E +

Sbjct: 1281 LTEEQNTLNISFQQATHQLEEKENQIKSMKA DIESLVTEKEALQKEGGNQQQAASE 1336

Query: 107 SYYSLRQYQSILEKQTSDLVLLHHHCKLKEDEVILYEEEMGNHNENTGEKLHLAQEQLAL 166

+ Q + L + + + L+ K K+ E+ +++ + N + L++++ A

Sbjct: 1337 KESCITQLKKELSENINAVTLMKEELKEKKVEISSLΞKQLTDLNVQLQNSISLSEKEAA- 1395

Query: 167 AGDKIASLERSLNLYRDKYQSSLSNIELLECQVKMLQGELGGIMGQEPENKGDHSKVRIY 226

I+SL + Y ++ L ++ L +V L E + Q + S+ +

Sbjct: 1396 ISSLRKQ YDEEKCELLDQVQDLSFKVDTLSKEKISALEQVDDWSNKFSEWK-K 1447

Query: 227 TSPCMIQEHQETQKRLS EVWQKVSQQDDLIQEL—RNK-LACSNALVLE 272

+ +HQ T K L E ++K Q + L +EL +NK C + +

Sbjct: 1448 KAQSRFTQHQNTVKELQIQLELKSKEAYEKDEQINLLKEELDQQNKRFDCLKGEMEDDKS 1507

Query: 273 -REKALIKLQADFASCTAT HRYPPSSSEECEDIKKILKHLQEQKDSQCLHVEEYQN 327

EK L+ + Ξ TA + + E E + ++LK+ +QKD E++

Sbjct: 1508 KMEKKESNLETELKSQTARIMELEDHITQKTIEIESLNEVLKNYNQQKDI EHKE 1561

Query: 328 LVKDLRVELEAVSEQKRNIMKDMMKLELDLHGLREETSAHIERKDKDI—TILQCRLQEL 385 LV+ L+ + + E+K N +K+ + L L A +E K K++ L + +E

Sbjct: 1562 LVQKLQ-HFQELGEEKDNRVKEAEEKILTLENQVYSMKAELETKKKELEHVNLSVKSKEE 1620

Query: 386 QLEFTETQKLTLKKDKFLQEKDEMLQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTV 445 +L+ E + L+ + + E+ ++ E+K+ ++ LL + +E E+Q TE ++

Sbjct: 1621 ELKALEDR LESES-AAKLAELKRKAEQKIAAIKKQLLSQMEEKEEQYKKGTESHLSE 1676

Query: 446 KEAKQDKSKEAECKALQAEVQKLKNSLEEAKQQERLAAQQAAQCK-EEAALAGCHLEDTQ 504

K + +E E L+ +++ +++S E R A AA + EEA GC + +

Sbjct: 1677 LNTKLQE-REREVHILEEKLKSVESSQSETLIVPRSAKNVAAYTEQEEADSQGCVQKTYE 1735

Query: 505 RKLQKGLLLDKQKADTIQELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLE 564

K+ +L + + + LQR Q +KE +++ + R + +E ++L A K

Sbjct: 1736 EKIS VLQRNLTEKEKLLQRVGQ--EKEETVSSHFEM--RCQYQERLIKLEHAEAKQH 1788

Query: 565 NSDKEKRQLQKTVAEQDMKMNDMLDRIKHQHREQG—SIKCK—LE EDLQ E 611

LQ+ + E++ K + ++ +H +E G +1+ K LE +D+Q E

Sbjct: 1789 EDQSMIGHLQEELEEKNKKYSLIV—AQHVEKEGGKNNIQAKQNLENVFDDVQKTLQEKE 1846

Query: 612 AT-KLLEDKREQLKKSKEHEKLMEG-ELEALRQEFKKKDKTLKENSR KLEEENENL 665

T ++LE K ++L +K + E+E L +++K + + R +L EEN

Sbjct: 1847 LTCQILEQKIKELDSCLVRQKEVHRVEMEELTSKYEKLQALQQMDGRNKPTELLEENTEE 1906

Query: 666 RAELQCCSTQLESSLN-KYNTSQQVIQDLNKEIALQKESLMSLQAQLDKALQKEKHYLQT 724 +++ +L S++ ++N + + +E + ++ LQ L + L+KE H +

Sbjct: 1907 KSKSHLVQPKLLΞNMEAQHNDLEFKLAGAEREKQKLGKEIVRLQKDL-RMLRKE-HQQEL 1964

Query: 725 TITKEAYDALSRKSAACQDDLTQALEKLNHVTSETKSLQQSLTQTQEKKAQLEEEIIAYE 784

I K+ YD R+ Q+ + LE L H ++ + +++ TQ +K+ +LE I +

Sbjct: 1965 EILKKEYDQ-EREEKIKQEQ—EDLE-LKHNSTLKQLMREFNTQLAQKEQELEMTI K 2017

Query: 785 ERMKKLNTELRKLRGFHQESELEVHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLR 844 E + K +L HQE E + KK+ E + + K+++ ++L A+EE++

Sbjct: 2018 ETINKAQEVEAELLESHQE ETNQLLKKIAEKDDDLKRTAKRYE EILDAREEEMT 2071

Query: 845 EFQEEMAALKENLLEDDKEPCCLPQWSVP-KDTCRLYRGNDQIMTNLEQWAKQQKVANEK 903

++ E L + ++ L Q P D + ++ T L Q K +++ K

Sbjct: 2072 AKVRDLQTQLEELQKKYQQK—LEQEENPGNDNVTIM ELQTQLAQ—KTTLISDSK 2123

Query: 904 LGNQ-LREQVNYIA-KLSGEKDHLHSVMV-HL 932 L Q REQ++ + +L + ++++ V HL

Sbjct: 2124 LKEQEFREQIHNLEDRLKKYEKNVYATTVGHL 2155

Score = 280 (42.0 bits), Expect = 5.2e-20, P = 5.2e-20

Identities = 209/938 (22%), Positives = 432/938 (46%)

Query: 3 DEAGERDREVS-SLNSKLLSLQLDIKN-LHDVC-KRQRKTLQDNQLCMEEAM-NSSHDKK 58 ++ ++ +E+ +L KLL + +K L + + +K Q N +E A NS+

Sbjct: 957 EKVKQKAKEMQETLKKKLLDQEAKLKKELENTALELSQKEKQFNAKMLEMAQANΞAGISD 1016

Query: 59 QAQALAFEESEVEFGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYSLRQYQSIL 118

L + E + S + H R+L + + + +++L EELQ + ++ +

Sbjct: 1017 AVSRLETNQKE-QIESLTEVHRRELNDV ISIWEKKLNQQAEELQ-EIHEIQLQEK— 1069

Query: 119 EKQTSDLV—LLHHHCKLKE-DEVILYEEEMGNHNENTGEKLHLAQEQLALAGDKIASLE 175 E++ ++L +L C+ +E ++ I + +E G + T +L +Q + + +A E

Sbjct: 1070 EQEVAELKQKILLFGCEKEEMNKEITWLKEEGVKQDTTLNELQEQLKQKSAHVNSLAQDE 1129

Query: 176 RSLNLYRDKYQSSLSNIELLECQVKMLQGELGGI--MGQEPENKGDHSKVRIYTSPCMIQ 233 L + +K + L N L E LQ +L + + +E + K ++ T+ Q Sbjct: 1130 TKLKAHLEKLEVDL-NKSLKENT—FLQEQLVELKMLAEEDKRKVSELTSKLKTTDEEFQ 1186

Query: 234 E HQETQKRLSEVWQKVSQQDDLIQELRNKL--AC—SNALVLEREKALIKLQADFA 285

H+++ K L + K + L +EL +L C + AL+ + LI + + Sbjct: 1187 SLKSSHEKSNKSLED KSLEFKKLSEELAIQLDICCKKTEALLEAKTNELINISSSKT 1243

Query: 286 SCTATH-RYPPSSSEECEDIKKILKHLQEQKDΞQCLHVEEYQNLVKDLRVELEAVSEQKR 344

+ + + + + ++ I + ++Q + E QN + + E+K Sbjct: 1244 NAILSRISHCQHRTTKVKEALLIKTCTVSELEAQLRQLTEEQNTLNISFQQATHQLEEKE 1303

Query: 345 NIMKDMMKLELD-LHGLREETSAHIERKDKDITILQCRLQELQLEFTET-QKLTLKKDKF 402

N +K M K +++ L +E + + + + + +L+ E +E +TL K++ Sbjct: 1304 NQIKSM-KADIESLVTEKEALQKEGGNQQQAASEKESCITQLKKELSENINAVTLMKEE- 1361

Query: 403 LQEKDEMLQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTVKEAKQDKSKEAECKALQ 462

L+EK + L K+LT + N L+ L +++ + L E K + + + L Sbjct: 1362 LKEKKVEISSLSKQLTDL-NVQLQNSISLSEKEAAISΞLRKQYDEEKCELLDQVQ—DLS 1418

Query: 463 AEVQKLKNSLEEAKQQERLAAQQAAQCKEEAALAGCHLEDTQRKLQKGLLLDKQKA 518

+V L A +Q + + ++ K++A ++T ++LQ L L ++A Sbjct: 1419 FKVDTLSKEKISALEQVDDWSNKFSEWKKKAQSRFTQHQNTVKELQIQLELKSKEAYEKD 1478

Query: 519 DTIQELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLENSDKEKRQLQKTVA 578

+ I L+ EL K + E ++ ++E+ L +L++ +L+ + Sbjct: 1479 EQINLLKEELDQQNKRFDCLKGEMEDDKSKMEKKESNLET ELKSQTARIMELEDHIT 1535

Query: 579 EQDMKMNDMLDRIKHQHREQGSIKCK-LEEDLQEATKLLEDKREQLKKSKEHEKLMEGEL 637

++ +++ + + +K+ + +Q 1+ K L + LQ +L E+K ++K+++E +E ++ Sbjct: 1536 QKTIEIESLNEVLKN-YNQQKDIEHKELVQKLQHFQELGEEKDNRVKEAEEKILTLENQV 1594

Query: 638 EALRQEFKKKDKTLKENSRKLEEENENLRAELQCCSTQLES-SLNKYNTSQQVIQDLNKE 696

+++ E + K K L+ + ++ + E L+A L+ +LES S K ++ + ++ Sbjct: 1595 YSMKAELETKKKELEHVNLSVKSKEEELKA-LE DRLESESAAKL AELKRKAEQK 1647

Query: 697 IALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALΞRKSAACQDDLTQALEKLNHVT 756

IA K+ L+S Q++ +KE+ Y + T + L+ K + ++ EKL V Sbjct: 1648 IAAIKKQLLS QME EKEEQYKKGT--ESHLSELNTKLQEREREVHILEEKLKSVE 1699

Query: 757 S ET KSLQQSLTQTQEKKAQLEEEII-AYEERMKKLNTELRKLRGFHQESELEV 808

S ET +S + T++++A + + YEE++ L L E E + Sbjct: 1700 SSQSETLIVPRSAKNVAAYTEQEEADSQGCVQKTYEEKISVLQRNLT EKEKLL 1752

Query: 809 HAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLREFQEEMAALKENLLEDDKEPCCLP 868

++ EE + + Q+Q L L E + E Q + L+E L E +K+ + Sb_jct: 1753 QRVGQEKEETVSSHFEMRCQYQERLIKLEHAEAKQHEDQSMIGHLQEELEEKNKKYSLIV 1812

Query: 869 QWSVPKDTCRLYRGNDQIMTNLEQ-WAKQQKVANEK-LGNQLREQ-VNYIAKLSGEKDHL 925

V K+ + N Q NLE + QK EK L Q+ EQ + + + + Sbjct: 1813 AQHVEKEGGK NNIQAKQNLENVFDDVQKTLQEKELTCQILEQKIKELDSCLVRQKEV 1869

Query: 926 HSV-MVHLQQENKKLK 940

H V M L + +KL+ Sbjct: 1870 HRVEMEELTSKYEKLQ 1885

Score = 227 (34.1 bits), Expect = 2.5e-14, P = 2.5e-14 Identities = 160/716 (22%), Positives = 318/716 (44%)

Query: 233 QEHQETQKRLSEVWQKVSQQDDLIQE-LRNKLACSNALV-LEREKALIKL-QADFASCTA 289

+E +TQ ++ +V + L + ++ L S++ L R + L + D S TA Sbjct: 53 RESGDTQSFAQKLQLRVPSVESLFRSPIKESLFRSSSKESLVRTSSRESLNRLDLDSSTA 112

Query: 290 THRYPPSSSEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAVSEQKRNIMKD 349

+ P E ED+ L +++ Q L + + R + + + + ++ Sbjct: 113 SFDPPSDMDSEAEDLVGNΞDSLNKEQLIQRLR—RMERSLSSYRGKYSELVTAYQMLQRE 170

Query: 350 MMKLELDLHGLREETSAHIERKDKDIT-ILQCRLQELQLEFTETQKLTLKKDKFLQEKDE 408

KL+ G+ ++ +DK + I + R +ELQ++ + L + D L+EKD+ Sbjct: 171 KKKLQ GILSQS QDKSLRRIAELR-EELQMDQQAKKHLQEEFDASLEEKDQ 219

Query: 409 MLQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTVKEAKQDKSKEAECKALQAE V 465

+ L+ +++ ++ L ++ + + +LE + ++++ E++ + + + V Sb_jct: 220 YISVLQTQVSLLKQRLRNGPMNVDVLKPLP-QLEPQAEVFTKEENPESDGEPVVEDGTΞV 278

Query: 466 QKLKNSLEEAKQQERLA—AQQAAQC-KEEAALAGCHLEDTQRKLQKGLL-LDKQKADTI 521

+ L+ + K+QE L ++ Q KE+ L E Q +L + L L+K K + Sb_jct: 279 KTLETLQQRVKRQENLLKRCKETIQSHKEQCTLLTSEKEALQEQLDERLQELEKIKDLHM 338

Query: 522 QELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLENΞDKEKRQLQKTVAEQD 581

E + + L+ ++ E+ + + E ++ E L E + R K + Q Sbjct: 339 AEKTKLITQLRDAKNLIEQLEQDKGMVIAETKRQMHETLEMKEEEIAQLRSRIKQMTTQG 398 Query: 582 MKMNDMLDRIKHQHREQGSIKCKLEEDLQEAT-KLLEDKREQLK KSKEHEKL-MEGE 636

++ + ++ + E+ + +EA KL + EQ+K K+ E E++ ++ E Sbjct: 399 EELREQKEKSERAAFEELEKALSTAQKTEEARRKLKAEMDEQIKTIEKTSEEERISLQQE 458

Query: 637 LEALRQEFKK-KDKTLKENSRKLEEENENLRAELQCCSTQLESSLNKYNTSQQVIQDLNK 695

L ++QE K+ +E KL++ +E EL +L L T ++ Q+ K Sbjct: 459 LSRVKQEVVDVMKKSΞEEQIAKLQKLHEK ELARKEQELTKKLQ TREREFQEQMK 512

Query: 696 EIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSRKSAACQDDLTQALEKLN-H 754

+AL+K L+ +K Q+ + + K+A S DL Q E Sbjct: 513 -VALEKSQSEYLKISQEKEQQESLALEELELQKKAILTESENKLR DLQQEAETYRTR 568

Query: 755 VTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKLNTELRKLRGFHQESELEV—HAFD 812

+ SL++SL QE K Q ++ + E K N E+ + H+ +ELE H D Sbjct: 569 ILELESSLEKSL QENKNQSKDLAVHLEAEKNKHNKEITVMVEKHK-TELESLKHQQD 624

Query: 813 KKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLRE FQEEMAALKENLLED-DK 862

E QVL+ +Q+Q +++ L K EQ +E FQ + + E LE D

Sbjct: 625 ALWTE-KLQVLK—QQYQTEMEKLREKCEQEKETLLKDKEIIFQAHIEEMNEKTLEKLDV 681

Query: 863 EPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVANEKLGNQLREQVNYIAKLSGEK 922

+ L S+ + + + ++ L Q ++L ++ EQ N+ + Sbjct: 682 KQTELE—SLSSELSEVLKARHKLEEELSVLKDQTDKMKQELEAKMDEQKNHHQQQVDSI 739

Query: 923 DHLHSVMVHLQQENKKLKKEIEEKKM 948

H V + Q+ K LK +1 + ++ Sbjct: 740 IKEHEVSI—QRTEKALKDQINQLEL 763

Score = 183 (27.5 bits), Expect = 1.3e-09, P = 1.3e-09 Identities = 132/584 (22%), Positives = 251/584 (42%)

Query: 409 MLQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTVKEAK-QDKSKEAECKALQAEVQK 467

M ++L++K+++ Q L + + +T M + + ++ E + Q Sbjct: 1 MFKKLKQKISEEQQQLQQALAPAQASSNSSTPTRMRSRTSSFTEQLDEGTPNRESGDTQS 60

Query: 468 LKNSLE-EAKQQERLAAQQAAQCKEEAALAGCHLEDTQRKLQKGLLLDKQKA—DTIQEL 524

L+ E L + ++ + + R+ L LD A D ++ Sbjct: 61 FAQKLQLRVPSVESLFRSPIKESLFRSSSKESLVRTSSRESLNRLDLDSΞTASFDPPSDM 120

Query: 525 QRELQMLQKESSMAEKEQTSNRKRVEELSL ELSEALRKLENSDKEKRQLQKTVAE 579

E + L S KEQ R R E SL + SE + + +EK++LQ +++ Sbjct: 121 DSEAEDLVGNSDSLNKEQLIQRLRRMERSLSSYRGKYSELVTAYQMLQREKKKLQGILSQ 180

Query: 580 -QDMKMNDMLDRIKHQHREQGSIKCKLEE DLQEATK LLEDKREQLKKSKEHEKL 632

QD + + + + +Q + K EE L+E + +L+ + LK+ + + Sbjct: 181 SQDKSLRRIAELREELQMDQQAKKHLQEEFDASLEEKDQYISVLQTQVSLLKQRLRNGPM 240

Query: 633 MEGELEALRQ-EFKKKDKTLKENSRKLEE ENENLRAELQCCSTQLESSLNKYNTSQQ 688

L+ L Q E + + T +EN E E+ L+ +++ N ++ Sbjct: 241 NVDVLKPLPQLEPQAEVFTKEENPESDGEPVVEDGTSVKTLETLQQRVKRQENLLKRCKE 300

Query: 689 VIQDLNKEIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSRKSAACQDDLTQA 748

IQ ++ L +LQ QLD+ LQ E ++ E +++ A +L + Sbjct: 301 TIQSHKEQCTLLTSEKEALQEQLDERLQ-ELEKIKDLHMAEKTKLITQLRDA—KNLIEQ 357

Query: 749 LEK-LNHVTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKLNTELRKLRGFHQESELE 807

LE+ V +ETK + + +T E K EEEI R+K++ T+ +LR Q+ + E Sbjct: 358 LEQDKGMVIAETK RQMHETLEMK EEEIAQLRSRIKQMTTQGEELR—EQKEKSE 409

Query: 808 VHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLREFQ EEMAALKENLLEDDKE 863

AF EE+ + QK + K+ A +EQ++ + EE +L++ L +E Sbjct: 410 RAAF EELEKALΞTAQKTEEARRKLKAEMDEQIKTIEKTSEEERISLQQELSRVKQE 465

Query: 864 PCCLPQWSVPKDTCRLYRGNDQIMTNLEQ-WAKQQKVANEKLGNQLR EQVNYIAK 917

+ + S + +L + +++ + EQ K+ + + Q++ Q Y+ K Sbjct: 466 VVDVMKKSSEEQIAKLQKLHEKELARKEQELTKKLQTREREFQEQMKVALEKSQSEYL-K 524

Query: 918 LSGEKDHLHSVMVH-LQQENKKLKKEIEEK KMKAENTRLCTKALGPSRTESTQREK 972

+S EK+ S+ + L+ + K + E E K + +AE R L S +S Q K Sbjct: 525 ISQEKEQQESLALEELELQKKAILTESENKLRDLQQEAETYRTRILELESSLEKSLQENK 584

Pedant information for DKFZphtes3_lgl3, frame 1

Report for DKFZphtes3_lgl3.1

[LENGTH] 1007

[MW] 117480.77

[pi] 5.90 [HOMOL] TREMBL:AF092090_1 product: "cpl51"; Rattus norvegicus cpl51 mRNA, partial eds.

0.0

[FUNCAT) 30.03 organization of cytoplasm [S. cerevisiae, YDL058w] 5e-15

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL058w]

5e-15

[FUNCAT] 09.10 nuclear biogenesis [S. cerevisiae, YDR356w] le-11

[FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YDR356w] le-11

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YDR356w] le-11

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKR095w] le-08

[FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YKR095w] le-08

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YLR309c] le-08

[FUNCAT] 1 genome replication, transcription, recombination and repair [M. annaschn, MJ1322] 4e-06

[FUNCAT] 09.13 biogenesis of chromosome structure [S. cerevisiae, YLR086w] 9e-06

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YHR023w

MYOl - myosm-1 isoform] 3e-04

[FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YHR023w MYOl - myosιn-1 isoform] 3e-04

[FUNCAT] 03.25 cytokinesis [S. cerevisiae, YHR023w MYOl - myosιn-1 isoform] 3e-04

[FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YJR134c] 5e-04

[EC] 3.6.1.32 Myosin ATPase le-16

[PIRKW] nucleus 3e-10

[PIRKW] phosphotransferase 6e-09

[PIRKW] duplication 2e-06

[PIRKW] citrullme 2e-12

[PIRKW] tandem repeat le-16

[PIRKW] endocytosis 2e-13

[PIRKW] heart 8e-13

[PIRKW] transmembrane protein le-13

[PIRKW] serine/threonine-specific protein kinase 6e-09

[PIRKW] zmc fmger 2e-13

[PIRKW] metal binding 2e-13

[PIRKW] DNA binding 4e-12

[PIRKW] muscle contraction le-16

[PIRKW] acetylated ammo end le-11

[PIRKW] actin binding le-16

[PIRKW] mitosis 5e-15

[PIRKW] microtubule binding 5e-15

[PIRKW] ATP le-16

[PIRKW] thick filament le-16

[PIRKW] phosphoprotein 4e-16

[PIRKW] skeletal muscle 2e-14

[PIRKW] calcium binding 2e-12

[PIRKW] alternative splicing le-16

[PIRKW] coiled coil le-16

[PIRKW] P-loop le-16

[PIRKW] heptad repeat 3e-10

[PIRKW] methylated amino acid le-16

[PIRKW] lmmunoglobulin receptor 2e-06

[PIRKW] peripheral membrane protein 2e-13

[PIRKW] cardiac muscle 8e-13

[PIRKW] hydrolase le-16

[PIRKW] microtubule 3e-10

[PIRKW] muscle 8e-13

[PIRKW] EF hand 2e-12

[PIRKW] cytoskeleton 2e-15

[PIRKW] hair 2e-12

[PIRKW] calmodulin binding 2e-13

[PIRKW] Golgi apparatus 3e-10

[SUPFAM] myosin heavy chain le-16

[SUPFAM] conserved hypothetical P115 protein le-07

[SUPFAM] centromere protein E 5e-15

[SUPFAM] unassigned Ser/Thr or Tyr-specific protein kinases 6e-09

[SUPFAM] calmodulin repeat homology 2e-12

[SUPFAM] myosin motor domain homology le-16

[SUPFAM] alpha-actimn actin-bmdmg domain homology 2e-07

[SUPFAM] plect 2e-07

[SUPFAM] trichohyalin 2e-12

[SUPFAM] pleckstrm repeat homology 8e-08

[SUPFAM] ribosomal protein S10 homology 2e-07

[SUPFAM] giantin 3e-13

[SUPFAM] protein kinase homology 6e-09

[SUPFAM] protein kinase C zmc-bmding repeat homology 8e-08

[SUPFAM] kinesin motor domain homology 5e-15

[SUPFAM] human early endosome antigen 1 2e-13

[SUPFAM] M5 protein le-07

[PROSITE] LEUCINE_ZIPPER 7

[PROSITE] MYRISTYL 2

[PROSITE] CAMP_PHOSPHO_SITE 2

[PROSITE] CK2 PHOSPHO_SITE 20 [PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 16

[PROSITE] ASN_GLYCOSYLATION 2

[KW] All_Alpha

[KW] LOW_COMPLEXITY 15.00 %

[KW] COILED COIL 42.40 %

SEQ MKDEAGERDREVSSLNSKLLSLQLDIKNLHDVCKRQRKTLQDNQLCMEEAMNSSHDKKQA

SEG xxxxxxxxxxxx

PRD ccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ QALAFEESEVEFGΞSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYSLRQYQSILEK

SEG xxxxxxxxxxxxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ QTSDLVLLHHHCKLKEDEVILYEEEMGNHNENTGEKLHLAQEQLALAGDKIASLERSLNL

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ YRDKYQSSLSNIELLECQVKMLQGELGGIMGQEPENKGDHSKVRIYTSPCMIQEHQETQK

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCC

SEQ RLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQADFASCTATHRYPPSSSEE

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS

SEQ CEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAVΞEQKRNIMKDMMKLELDLHGL

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ REETSAHIERKDKDITILQCRLQELQLEFTETQKLTLKKDKFLQEKDEMLQELEKKLTQV

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCC CCCCCCCCCCCCCC

SEQ QNSLLKKEKELEKQQCMATELEMTVKEAKQDKSKEAECKALQAEVQKLKNSLEEAKQQER

SEG ... xxxxxxxxxx xxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ LAAQQAAQCKEEAALAGCHLEDTQRKLQKGLLLDKQKADTIQELQRELQMLQKESSMAEK

SEG xxxxxxxxxxxxxxxx xxxxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCC

SEQ EQTSNRKRVEELSLELSEALRKLENSDKEKRQLQKTVAEQDMKMNDMLDRIKHQHREQGS

SEG xxxxxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ IKCKLEEDLQEATKLLEDKREQLKKSKEHEKLMEGELEALRQEFKKKDKTLKENSRKLEE

SEG xxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCC

SEQ ENENLRAELQCCSTQLEΞSLNKYNTSQQVIQDLNKEIALQKESLMSLQAQLDKALQKEKH

SEG xxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCC CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ YLQTTITKEAYDALSRKSAACQDDLTQALEKLNHVTSETKSLQQSLTQTQEKKAQLEEEI

SEG xxxxxxxxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCC

SEQ IAYEERMKKLNTELRKLRGFHQESELEVHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKE

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCC

SEQ EQLREFQEEMAALKENLLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVA

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS

SEQ NEKLGNQLREQVNYIAKLSGEKDHLHSVMVHLQQENKKLKKEIEEKKMKAENTRLCTKAL

SEG xxxxxxxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ GPSRTESTQREKVCGTLGWKGLPQDMGQRMDLTKYIGMPHCPGSSYC SEG PRD cchhhhhhhhhhhhhhhhcccccccccchhhhhheeecccccccccc COILS

Prosite for DKFZphtes3_lgl3.1

PS00001 52->56 ASN_GLYCOSYLATION PDOC00001 PS00001 684->688 ASN_GLYCOSYLATION PDOC00001 PS00004 240->244 CAMP_PHOSPHO_SITE PDOC00004 PS00004 415->419 CAMP_PHOSPHO_SITE PDOC00004 PS00005 74->77 PKC_PHOSPHO_SITE PDOC00005 PS00005 110->113 PKC_PHOSPHO_SITE PDOC00005 PS00005 238->241 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 290->293 PKC_PHOΞPHO_SITE PDOC00005 PS00005 392->395 PKC_PHOSPHO_SITE PDOC00005 PS00005 396->399 PKC_PHOSPHO_SITE PDOC00005 PS00005 444->447 PKC_PHOSPHO_SITE PDOC00005 PS00005 503->506 PKC_PHOSPHO_SITE PDOC00005 PS00005 544->547 PKC_PHOSPHO_SITE PDOC00005 PS00005 566->569 PKC_PHOSPHO_SITE PDOC00005 PS00005 600->603 PKC_PHOSPHO_SITE PDOC00005 PS00005 650->653 PKC_PHOSPHO_SITE PDOC00005 PS00005 655->658 PKC_PHOSPHO_SITE PDOC00005 PS00005 735->738 PKC_PHOSPHO_SITE PDOC00005 PS00005 876->879 PKC_PHOSPHO_SITE PDOC00005 PS00005 968->971 PKC_PHOSPHO_SITE PDOC00005 PS00006 39->43 CK2_PHOSPHO_SITE PDOC00006 PS00006 53->57 CK2_PHOSPHO_SITE PDOC00006 PS00006 68->72 CK2_PHOSPHO_SITE PDOC00006 PS00006 116-M20 CK2_PHOSPHO_SITE PDOC00006 PS00006 190->194 CK2_PHOSPHO_SITE PDOC00006 PS00006 250->254 CK2_PHOSPHO_SITE PDOC00006 PS00006 296->300 CK2_PHOSPHO_SITE PDOC00006 PS00006 439->443 CK2_PHOSPHO_SITE PDOC00006 PS00006 444->448 CK2_PHOSPHO_SITE PDOC00006 PS00006 471->475 CK2_PHOSPHO_SITE PDOC00006 PS00006 520->524 CK2_PHOSPHO_SITE PDOC00006 PS00006 536->540 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 566->570 CK2_PHOSPHO_SITE PDOC00006 PS00006 576->580 CK2_PHOSPHO_SITE PDOC00006 PS00006 650->654 CK2_PHOSPHO_SITE PDOC00006 PS00006 674->678 CK2_PHOSPHO_SITE PDOC00006 PS00006 804->808 CK2_PHOSPHO_SI E PDOC00006 PS0000Θ 888->892 CK2_PHOSPHO_SITE PDOC00006 PS00006 963->967 CK2_PHOSPHO_SITE PDOC00006 PS00006 968->972 CK2_PHOSPHO_SITE PDOC00006 PS00007 135->143 TYR_PHOSPHO_SITE PDOC00007 PΞ00008 207->213 MYRISTYL PDOC00008 PS00008 599->605 MYRISTYL PDOC00008 PS00029 83->105 LEUCINE_ZIPPER PDOC00029 PS00029 90->112 LEUCINE_ZIPPER PDOC00029 PS00029 97->119 LEUCINE_ZIPPER PDOC00029 PS00029 104->126 LEUCINE_ZIPPER PDOC00029 PS00029 403->425 LEUCINE_ZIPPER PDOC00029 PS00029 410->432 LEUCINE_ZIPPER PDOC00029 PS00029 918->940 LEUCINE ZIPPER PDOC00029

(No Pfam data available for DKFZphtes3_lgl3.1)

DKFZphtes3_lkll

group: cell structure and motility DKFZphtes3_lkll encodes a novel 589 amino acid protein with strong similarity to Mus musculus actin-binding protein (ENC-1) .

Ectoderm-neural cortex-1 protein (ENC-1) is an early and highly specific marker of neural induction in vertebrates. The protein is related to the kelch family proteins and is expressed during early gastrulation in the prospective neuroectodermal region of the epiblast and later m development throughout the nervous system (NS) . ENC-1 functions as an actin-binding protein organising the actin cytoskeleton during neural differentiation and development of the NS . The novel protein is highly similar to ENC-1.

The new protein can find application in modulation of cyto skeleton organisation in human testicular cells. strong similarity to mouse ENC-1 complete cDNA, compete eds, EST hits

Sequenced by DKFZ

Locus: unknown

Insert length: 3525 bp

Poly A stretch at pos. 3515, polyadenylation signal at pos. 3499

1 GGTGGAGAGC CGGCCGACGG GAGCCGCGGC GGAGCCTGTT GAGCTCGCGC

51 GGGCTGCCGG GAGTGGTCTC TGAGGCGGCG GCGGCGGCGG GGATCGTCTC

101 CGGCACTGGC GCACCATGTC GGTCAGTGTC CATGAGACCC GCAAGTCGCG

151 GAGCAGCACG GGGTCCATGA ACGTCACCCT CTTCCACAAG GCCTCCCACC

201 CGGACTGTGT GCTGGCCCAC CTCAACACGC TTCGCAAGCA CTGCATGTTC

251 ACCGACGTCA CACTCTGGGC GGGCGACCGT GCCTTCCCCT GTCACCGTGC

301 CGTGCTGGCC GCCTCTAGCC GCTATTTTGA GGCCATGTTC AGCCATGGCC

351 TTCGGGAGAG CCGGGATGAC ACTGTCAACT TCCAGGACAA CCTGCACCCG

401 GAGGTGCTGG AGCTGCTGCT GGACTTTGCC TACTCCTCAC GCATCGCCAT

451 CAACGAGGAG AACGCTGAGT CACTGCTGGA GGCAGGCGAC ATGCTGCAGT

501 TCCACGATGT GCGGGATGCT GCCGCCGAGT TCCTGGAGAA GAACCTTTTC

551 CCCTCCAACT GCCTGGGCAT GATGCTGCTC TCGGACGCCC ACCAGTGCCG

601 CCGGCTGTAT GAGTTCTCCT GGCGCATGTG CCTGGTGCAC TTTGAGACGG

651 TGAGGCAGAG CGAGGACTTC AACAGCCTGT CCAAGGACAC ACTGCTGGAC

701 CTCATCTCGA GTGATGAGCT GGAGACCGAG GACGAGCGGG TGGTCTTCGA

751 GGCCATCCTC CAGTGGGTGA AGCACGACCT GGAGCCACGG AAGGTCCACT

801 TGCCCGAGCT CCTCCGCAGC GTGCGTCTGG CCTTGCTGCC GTCCGACTGC

851 CTGCAGGAGG CCGTCTCCAG CGAGGCCCTC CTCATGGCAG ACGAGCGCAC

901 CAAGCTTATC ATGGATGAGG CCCTGCGCTG CAAGACCAGG ATCCTGCAGA

951 ATGATGGCGT GGTCACCAGC CCCTGTGCCC GGCCACGCAA GGCGGGCCAC

1001 ACGCTACTCA TCCTGGGGGG CCAGACCTTC ATGTGTGACA AGATCTACCA

1051 GGTGGACCAC AAGGCCAAGG AGATCATCCC CAAGGCCGAC CTGCCCAGCC

1101 CCCGGAAGGA GTTCAGCGCC TCAGCGATCG GCTGCAAGGT CTATGTGACG

1151 GGGGGCAGGG GCTCCGAGAA CGGGGTCTCC AAGGATGTCT GGGTGTACGA

1201 CACCGTACAT GAGGAATGGT CCAAGGCGGC GCCCATGCTG ATTGCCCGCT

1251 TTGGCCATGG CTCAGCTGAG CTGGAGAACT GCCTCTATGT GGTGGGGGGA

1301 CACACATCCC TGGCAGGGGT CTTCCCGGCC TCGCCTTCTG TCTCCCTGAA

1351 ACAAGTGGAG AAATACGACC CTGGGGCCAA CAAGTGGATG ATGGTGGCCC

1401 CCTTGCGGGA TGGCGTCAGC AATGCCGCAG TGGTGAGTGC CAAGCTGAAG

1451 CTCTTTGTTT TCGGAGGAAC CAGCATCCAC CGGGACATGG TGTCCAAGGT

1501 CCAGTGCTAT GACCCCTCGG AGAACAGGTG GACGATCAAG GCCGAGTGCC

1551 CCCAGCCTTG GCGGTACACA GCCGCTGCCG TCCTGGGCAG CCAGATCTTC

1601 ATCATGGGAG GTGACACGGA ATTCACAGCC GCCTCGGCCT ACCGCTTTGA

1651 CTGTGAGACC AACCAGTGGA CGCGGATTGG GGACATGACT GCCAAGCGCA

1701 TGTCCTGCCA TGCCCTGGCT TCCGGCAACA AGCTCTATGT GGTCGGGGGC

1751 TACTTTGGGA CCCAGAGGTG TAAGACTCTG GACTGCTATG ACCCCACTTC

1801 AGATACATGG AACTGCATCA CCACAGTGCC CTACTCACTT ATCCCCACGG

1851 CCTTTGTCAG CACCTGGAAG CACCTGCCCG CGTGAGGAGC ACCTGCTGAG

1901 CCCAGCCAGA CCGCGGCCTT CAGTGTCACA GCGTGGCCTT GCTTGTCTGC

1951 CACAGCGGGA GCTAAGCCGG CCCTGGGCCA GCACTCCGAG AGGTGGAAGG

2001 GGCCCTGCCA GCTCTGGGGA GCAGCAGCCT TGGGCTGTTC TGAGCTTTAG

2051 GCAAGAGAAG AGAAGCATCT CTTGCATCCG TGCCCCTGGG GGCCTCTTCA

2101 GCTTTGCAGT GGTTTGTGGG AAGACATACC TCCCAGAGGG GCATGGACTG

2151 CCACCAGGAC TGACCCTGGC GTCGGGGAGA AGGACACTTG CAGAGCCTTG

2201 AGATCACCTG TTTGGCAGGT CCTGGACTGG GGCCGGGCAG GCAGGGGCAG

2251 GGAGGCGCCC CGGGTGGGCT TTGGGGCTGC GGCACTGCCA CACATCCTTT

2301 CCCTCCTGGC CTGCCCTGCT GGGGCTCTAC TGCCATCTAT AGATGGTGTC

2351 CTGGGCCTGG GAAACTAGGT TCCCAGGGGT TGAGACCAGA AAGGTGACCA

2401 AGACAGATTT TTTAAGGTGC AGAAACTGCA GGGGGGCCTC AGTGACATCC

2451 ATGAGGCCTT ATTAGCAAAG GACACCCAGA CCTCCAAGGT TTGTGGGCCC

2501 CTTCCACAAA GCTGTAAGTC CCAGCCCACC TACTCAGGGC CTTGCTCAGT

2551 GCTGTGGCCC GGTGGGGACA CAGTTGCTCG TGGCCACTCA GTGGAGCTGG

2601 GCCTGCAGCA GACTCAAGGC TCCGAGTGCC CTGGGGGTCA CCCCTCCCCT

2651 CCCCTCCTCA GAGCCCACCC TGAGAGGCAG CAGTGACCCC CATGGCACAC

2701 ACCTGCCAAC AGCACTGGGG GCTTCTCCCC AGGAGACCAC GCTGCCCTCC 2751 AAGACCAGGA GCAGCTGTGA GCTGGAGACA GCAGAGGGAC CCCAGGGTGT

2801 CCCCTGCAGA TCCCACCAGG GCCGCATCCA TCTCAGTGTG GAGGACAGTG

2851 ACGGGACCCT CACCATCCTC TTGCGTTTTG GCCCCCATTT GCTCCCTGAG

2901 CTCCAAGATA AGAATGGCCC CGAGAGAACT GCTGAACATT TGTTCATTGC

2951 TGTCACCTCC TGAGTCACTG GGGTCCCTCA CCAGCACCTC CCTGACACCT

3001 GGGCTATGGA GAGGTTGGCG CCTGTCAGTG ACCATCCTAA TGCCTCTCGC

3051 TCACTCCCAA GCCACCATTT GAGAGGGAGG GGTGTTGGTG CCCTGACAGG

3101 GACTGGGCAG GGTGTCCAAA CTTGGGGCTT CCCAGGCACC TGCAGTGTGA

3151 ACACTGCTTG GCTGGCTCAA GATTAGGGCC GCGGAGGGGG CTGTGCACAT

3201 ACCAGTTACT TAAGCAGCCA CGAGTGTCCC CCATGCCTTG GTGCGGGTCC

3251 TGGAGGCCTC TTGGGGGTGG GACCTTTGGG CAGGGTTTGC CCACTGACGC

3301 GCCCGCCATG GGGCACTGGC TGCATGGGGC TCCTTGGACC CTGTAGACCC

3351 AGCAGGAGCC TGGCCGCGGG GACTGCAGGG AGGGTGCCTG GACCCGTGGG

3401 GTTGCTTCAT TGAGATAAAG CACACTTATC ACATAGCACA AAGGACGTGC

3451 CATGGTGCTT TCCCCAAAAG TTGTGTTGCT TTTATCAGTT TTCTAACTTA

3501 ATAAAAAGAG TTGAGAAAAA AAAAA

BLAST Results

No BLAST result

Medline entries

98350113:

Cloning of human ENC-1 and evaluation of its expression and regulation in nervous system tumors.

97252647:

ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-bmdmg protein.

98234394:

NRP/B, a novel nuclear matrix protein, associates with pllO(RB) and is involved in neuronal differentiati

Peptide information for frame 2

ORF from 116 bp to 1882 bp; peptide length: 589 Category: strong similarity to known protein Classification: Cell structure/motility

1 MSVSVHETRK SRSSTGSMNV TLFHKASHPD CVLAHLNTLR KHCMFTDVTL 51 WAGDRAFPCH RAVLAASSRY FEAMFSHGLR ESRDDTVNFQ DNLHPEVLEL 101 LLDFAYSSRI AINEENAESL LEAGDMLQFH DVRDAAAEFL EKNLFPSNCL 151 GMMLLSDAHQ CRRLYEFSWR MCLVHFETVR QSEDFNSLSK DTLLDLISSD 201 ELETEDERVV FEAILQWVKH DLEPRKVHLP ELLRSVRLAL LPSDCLQEAV 251 SSEALLMADE RTKLIMDEAL RCKTRILQND GVVTSPCARP RKAGHTLLIL 301 GGQTFMCDKI YQVDHKAKEI IPKADLPSPR KEFSASAIGC KVYVTGGRGS 351 ENGVSKDVWV YDTVHEEWSK AAPMLIARFG HGSAELENCL YVVGGHTSLA 401 GVFPASPSVS LKQVEKYDPG ANKWMMVAPL RDGVSNAAVV SAKLKLFVFG 451 GTSIHRDMVS KVQCYDPSEN RWTIKAECPQ PWRYTAAAVL GSQIFIMGGD 501 TEFTAASAYR FDCETNQWTR IGDMTAKRMS CHALASGNKL YVVGGYFGTQ 551 RCKTLDCYDP TSDTWNCITT VPYSLIPTAF VSTWKHLPA

BLASTP hits

Entry MMU65079__1 from database TREMBL: gene: "ENC-1"; product: "actin-bindmg protein"; Mus musculus actin-binding protein (ENC-1) mRNA, complete eds.

Score = 2402, P = 1.9e-249, identities = 440/589, positives = 513/589

Entry AF059611_1 from database TREMBLNEW: gene: "NRPB"; product: "nuclear matrix protein NRP/B"; Homo sapiens nuclear matrix protein NRP/B (NRPB) mRNA, complete eds.

Score = 2400, P = 3.0e-249, identities = 440/589, positives = 512/589

Entry AF010314_1 from database TREMBL: gene: "PIG10"; product: "PiglO"; Homo sapiens PiglO (PIG10) mRNA, complete eds .

Score = 1745, P = 7.8e-180, identities = 335/507, positives = 403/507 Entry KELC_DROME from database SWISSPROT:

RING CANAL PROTEIN (KELCH PROTEIN) . >TREMBL : DMRCPA_1 product: "ring canal protein"; Drosophila melanogaster ring canel protein and ORF2 mRNA, complete eds.

Score = 672, P = 3.9e-66, identities = 168/536, positives = 257/536

Alert BLASTP hits for DKFZphtes3_lkll, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphtes3_lkll, frame 2

Report for DKFZphtes3_lkll .2

[LENGTH] 589

[MW] 65923.45

[pi] 6.10

[HOMOL] TREMBL:MMU65079_1 gene: "ENC-1"; product: "actm-binding protein"; Mus musculus actm-binding protein (ENC-1) mRNA, complete eds. 0.0

[FUNCAT] 10.05.99 other pheromone response activities [S. cerevisiae, YHR158c] 2e-09

[BLOCKS] BL01016D Glycoprotease family proteins

[PIRKW] zmc f ger le-08

[PIRKW] DNA binding le-08

[PIRKW] transcription factor le-08

[SUPFAM] POZ domain homology 3e-68

[SUPFAM] vaccinia virus 59K Hindlll-C protein le-15

[SUPFAM] A55R protein 5e-29

[SUPFAM] hypothetical protein YHR158c 4e-08

[SUPFAM] A55R protein middle region homology 5e-29

[SUPFAM] myxoma virus M9-R protein le-14

[SUPFAM] A55R protein carboxyl-terminal homology 5e-29

[KW] Alpha_Beta

SEQ MSVSVHETRKSRSSTGSMNVTLFHKASHPDCVLAHLNTLRKHCMFTDVTLWAGDRAFPCH PRD cccccccccccccccccceeeeeeccccchhhhhhhhhhhhhhhhheeeeeecccchhhh

SEQ RAVLAASSRYFEAMFSHGLRESRDDTVNFQDNLHPEVLELLLDFAYSSRIAINEENAESL PRD hcccccccccccccccccchhhhhheeeeccccchhhhhhhhhhhhccceeehhhhhhhh

SEQ LEAGDMLQFHDVRDAAAEFLEKNLFPSNCLGMMLLSDAHQCRRLYEFSWRMCLVHFETVR PRD hhhhhhhhhhhhhhhhhhhhhhhccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ QSEDFNSLSKDTLLDLISSDELETEDERVVFEAILQWVKHDLEPRKVHLPELLRSVRLAL PRD hhhhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhhc

SEQ LPSDCLQEAVSSEALLMADERTKLIMDEALRCKTRILQNDGVVTSPCARPRKAGHTLLIL PRD ccchhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhcccccccccccccccccceeeeee

SEQ GGQTFMCDKIYQVDHKAKEIIPKADLPSPRKEFSASAIGCKVYVTGGRGSENGVSKDVWV PRD cccccccceeeeeccccccccccccccccccceeeeeeceeeeeecccccccccceeeee

SEQ YDTVHEEWSKAAPMLIARFGHGΞAELENCLYVVGGHTSLAGVFPASPΞVSLKQVEKYDPG PRD cccccccccccccccccccccceeeccceeeeecccccccccccccccccccceeecccc

SEQ ANKWMMVAPLRDGVΞNAAVVSAKLKLFVFGGTSIHRDMVSKVQCYDPSENRWTIKAECPQ PRD ccceeeeccccccccceeeeeccceeeeeccccccccccceeeecccccccccccccccc

SEQ PWRYTAAAVLGSQIFIMGGDTEFTAASAYRFDCETNQWTRIGDMTAKRMSCHALASGNKL PRD ccccceeeeecceeeeecccccccccceeecccccccceeeccccccccceeeeecccee

SEQ YVVGGYFGTQRCKTLDCYDPTSDTWNCITTVPYSLIPTAFVSTWKHLPA PRD eeecccccccccccccccccccccceeeeeccccccceeeeeecccccc

(No Prosite data available for DKFZphtes3_lkll .2) (No Pfam data available for DKFZphtes3 lkll.2) DKFZphtes3_ln3

group: signal transduction

DKFZphtes3_ln3 encodes a novel 1196 ammo acid protein with similarity to S. pombe Tupl protein.

The protein contains 1 WD-40 repeat, which is typical for the beta-transducin subunit of G- protems . The beta subunits seem to be required for the replacement of GDP by GTP as well as for membrane anchoring and receptor recognition. In addition, a RGD site is present.

The new protein can find application in modulating/blocking G-protein-dependent pathways.

similarity to Tuplp complete cDNA, complete eds, EST hits

Sequenced by DKFZ

Locus: /map="6q24"

Insert length: 5277 bp

Poly A stretch at pos. 5267, polyadenylation signal at pos. 5244

1 GCTGCATAAA GCTGAGAGAT GCCTACAGCT GAGAGTGAAG CAAAAGTAAA

51 AACCAAAGTT CGCTTTGAAA AATTGCTTAA GACCCACAGT GATCTAATGC

101 GTGAAAAGAA AAAACTGAAG AAAAAACTTG TCAGGTCTGA AGAAAACATC

151 TCACCTGACA CTATTAGAAG CAATCTTCAC TATATGAAAG AAACTACAAG

201 TGATGATCCC GACACTATTA GAAGCAATCT TCCCCATATT AAAGAAACTA

251 CAAGTGATGA TGTAAGTGCT GCTAACACTA ACAACCTGAA GAAGAGCACG

301 AGAGTCACTA AAAACAAATT GAGGAACACA CAGTTAGCAA CTGAAAATCC

351 TAATGGTGAT GCTAGTGTAG AGGAAGACAA ACAAGGAAAG CCAAATAAAA

401 AGGTGATAAA GACGGTGCCC CAGTTGACTA CACAAGACCT GAAACCGGAA

451 ACTCCTGAGA ATAAGGTTGA TTCTACACAC CAGAAAACAC ATACAAAGCC

501 ACAGCCAGGC GTTGATCATC AGAAAAGTGA GAAGGCAAAT GAGGGAAGAG

551 AAGAGACTGA TTTAGAAGAG GATGAAGAAT TGATGCAAGC ATATCAGTGC

601 CATGTAACTG AAGAAATGGC AAAGGAGATT AAGAGGAAAA TAAGAAAGAA

651 ACTGAAAGAA CAGTTGACTT ACTTTCCCTC AGATACTTTA TTCCATGATG

701 ACAAACTAAG CAGTGAAAAA AGGAAAAAGA AAAAGGAAGT TCCAGTCTTC

751 TCTAAAGCTG AAACAAGTAC ATTGACCATC TCTGGTGACA CAGTTGAAGG

801 TGAACAAAAG AAAGAATCTT CAGTTAGATC AGTTTCTTCA GATTCTCATC

851 AAGATGATGA AATAAGCTCA ATGGAACAAA GCACAGAAGA CAGCATGCAA

901 GATGATACAA AACCTAAACC AAAAAAAACA AAAAAGAAGA CTAAAGCAGT

951 TGCAGATAAT AATGAAGATG TTGATGGTGA TGGTGTTCAT GAAATAACAA

1001 GCCGAGATAG CCCGGTTTAT CCCAAATGTT TGCTTGATGA TGACCTTGTC

1051 TTGGGAGTTT ACATTCACCG AACTGATAGA CTTAAGTCAG ATTTTATGAT

1101 TTCTCACCCA ATGGTAAAAA TTCATGTGGT TGATGAGCAT ACTGGTCAAT

1151 ATGTCAAGAA AGATGATAGT GGACGGCCTG TTTCATCTTA CTATGAAAAA

1201 GAGAATGTGG ATTATATTCT TCCTATTATG ACCCAGCCAT ATGATTTTAA

1251 ACAGTTAAAA TCAAGACTTC CAGAGTGGGA AGAACAAATT GTATTTAATG

1301 AAAATTTTCC CTATTTGCTT CGAGGCTCTG ATGAGAGTCC TAAAGTCATC

1351 CTGTTCTTTG AGATTCTTGA TTTCTTAAGC GTGGATGAAA TTAAGAATAA

1401 TTCTGAGGTT CAAAACCAAG AATGTGGCTT TCGGAAAATT GCCTGGGCAT

1451 TTCTTAAGCT TCTGGGAGCC AATGGAAATG CAAACATCAA CTCAAAACTT

1501 CGCTTGCAGC TATATTACCC ACCTACTAAG CCTCGATCCC CATTAAGTGT

1551 TGTTGAGGCA TTTGAATGGT GGTCAAAATG TCCAAGAAAT CATTACCCAT

1601 CAACACTGTA CGTAACTGTA AGAGGACTGA AAGTTCCAGA CTGTATAAAG

1651 CCATCTTACC GCTCTATGAT GGCTCTTCAG GAGGAAAAAG GTAAACCAGT

1701 GCATTGTGAA CGTCACCATG AGTCAAGCTC AGTAGACACA GAACCTGGAT

1751 TAGAAGAGTC AAAGGAAGTA ATAAAGTGGA AACGACTCCC TGGGCAGGCT

1801 TGCCGTATCC CAAACAAACA CCTCTTCTCA CTAAATGCAG GAGAACGAGG

1851 ATGTTTTTGT CTTGATTTCT CCCACAATGG AAGAATATTA GCAGCAGCTT

1901 GTGCCAGCCG GGATGGATAT CCAATTATTT TATATGAAAT TCCTTCTGGA

1951 CGTTTCATGA GAGAATTGTG TGGCCACCTC AATATCATTT ATGATCTTTC

2001 CTGGTCAAAA GATGATCACT ACATCCTTAC TTCATCATCT GATGGCACTG

2051 CCAGGATATG GAAAAATGAA ATAAACAATA CAAATACTTT CAGAGTTTTA

2101 CCTCATCCTT CTTTTGTTTA CACGGCTAAA TTCCATCCAG CTGTAAGAGA

2151 GCTAGTAGTT ACAGGATGCT ATGATTCCAT GATACGGATA TGGAAAGTTG

2201 AGATGAGAGA AGATTCTGCC ATATTGGTCC GACAGTTTGA TGTTCACAAA

2251 AGTTTTATCA ACTCACTTTG TTTTGATACT GAAGGTCATC ATATGTATTC

2301 AGGAGATTGT ACAGGGGTGA TTGTTGTTTG GAATACCTAT GTCAAGATTA

2351 ATGATTTGGA ACATTCAGTG CACCACTGGA CTATAAATAA GGAAATTAAA

2401 GAAACTGAGT TTAAGGGAAT TCCAATAAGT TATTTGGAGA TTCATCCCAA

2451 TGGAAAACGT TTGTTAATCC ATACCAAAGA CAGTACTTTG AGAATTATGG

2501 ATCTCCGGAT ATTAGTAGCA AGGAAGTTTG TAGGAGCAGC AAATTATCGG

2551 GAGAAGATTC ATAGTACTTT GACTCCATGT GGGACTTTTC TGTTTGCTGG

2601 AAGTGAGGAT GGTATAGTGT ATGTTTGGAA CCCAGAAACA GGAGAACAAG 2651 TAGCCATGTA TTCTGACTTG CCATTCAAGT CACCCATTCG AGACATTTCT 2701 TATCATCCAT TTGAAAATAT GGTTGCATTC TGTGCATTTG GGCAAAATGA 2751 GCCAATTCTT CTGTATATTT ACGATTTCCA TGTTGCCCAG CAGGAGGCTG 2801 AAATGTTCAA ACGCTACAAT GGAACATTTC CATTACCTGG AATACACCAA 2851 AGTCAAGATG CCCTATGTAC CTGTCCAAAA CTACCCCATC AAGGCTCTTT 2901 TCAGATTGAT GAATTTGTCC ACACTGAAAG TTCTTCAACG AAGATGCAGC 2951 TAGTAAAACA GAGGCTTGAA ACTGTCACAG AGGTGATACG TTCCTGTGCT 3001 GCAAAAGTCA ACAAAAATCT CTCATTTACT TCACCACCAG CAGTTTCCTC 3051 ACAACAGTCT AAGTTAAAGC AGTCAAACAT GCTGACCGCT CAAGAGATTC 3101 TACATCAGTT TGGTTTCACT CAGACCGGGA TTATCAGCAT AGAAAGAAAG 3151 CCTTGTAACC ATCAGGTAGA TACAGCACCA ACGGTAGTGG CTCTTTATGA 3201 CTACACAGCG AATCGATCAG ATGAACTAAC CATCCATCGC GGAGACATTA 3251 TCCGAGTGTT TTTCAAAGAT AATGAAGACT GGTGGTATGG CAGCATAGGA 3301 AAGGGACAGG AAGGTTATTT TCCAGCTAAT CATGTGGCTA GTGAAACACT 3351 GTATCAAGAA CTGCCTCCTG AGATAAAGGA GCGATCCCCT CCTTTAAGCC 3401 CTGAGGAAAA AACTAAAATA GAAAAATCTC CAGCTCCTCA AAAGCAATCA 3451 ATCAATAAGA ACAAGTCCCA GGACTTCAGA CTAGGCTCAG AATCTATGAC 3501 ACATTCTGAA ATGAGAAAAG AACAGAGCCA TGAGGACCAA GGACACATAA 3551 TGGATACACG GATGAGGAAG AACAAGCAAG CAGGCAGAAA AGTCACTCTA 3601 ATAGAGTAAA GAATTGAAGA AAAGTTAAGA GCTGCCGAAA TGCACAGAGG

3651 TGAAAATGAC AAACCAAATG GAATTTCTCT TCAGAGTTCA GAATTTTCAG 3701 ATACTAAGGA GGAAGAAAGG ATCCACTACT TCTTGTTCTT ATGAATGACT 3751 CTAGAAAAAT CAGAATCAAG TTGTGGGTGG AAAAATCAAC GTGGCCTTTG 3801 AGTTCAGTTG TTATAAACCA TTGTGACTAT TGTTGGTCAA AGTATTGGTA 3851 CTTATATTGT TAGTAATTGC ATCATAATTA CATTACCAGT GTTGGAAAAC 3901 TAATGAAGAA AACACTGTAA TTGCTACTCA GCAAATGTGA ATAAAAGGTG 3951 TTTGCGTTAT TAGGATGTCT GTTAAGTAAT CATTTAATAT TATTATATTG 4001 GTAATGGTTG TATGTGTGAT GCTATGCCCA GAATATGAAG TATCTGTTTT 4051 TGAAATTCAC TTTATTTAAA AGATAAGCAG CTGACTGGGC ACGGTGCCTC 4101 ATGCCTGTAA TCCTAGCACC TTGGGAGGCT GAGGCAGGTG GATCACCTAA 4151 GGTCAGGAGT TCAACAACAC CAGCCTGACC AACATGGTGA AACCCCATCT 4201 CTACTAAAAA TACAAAAATC AGCCGGGTCT CATGGCAGGC ACCTGTAATC 4251 CCATCTACTG AGGCAGGAGA ATTGCTTGAC CCAGGAGGCA GAGGTTGCAG 4301 TGAGCCAAGA TCACGCCATT GCACTCCAGC CTGGGGGACA GAGCAAGACT 4351 CTATCTCCAA AAAACAAAAA AGATAAGCAG CTTTAGAATA TGGCGCATTC 4401 AAAACAGTCT CAGTAACAAA GACATTAAAA GAAAACAATT TACTTTCTAA 4451 TTAAAATTTT GTGTTTCTTA AGATCAAATC ATATAGGTAA CTTCATAGAC 4501 CTAAATTAAA AGTGATTTTT GGCTGGACTG GCAACAATGT TCCCAATGTC 4551 TTTACTTTTT AAAAAAGGCT TTTCATATTT AAGCACATAC CTATTTTGTA

4601 GACTTACATT GTTTAATATT TATTTTAATC TTAATATTTT TACATTATTA 4651 TATTGCATTA TTTATTTTTT CTAAGTTCCA GAATAATAGT GTCATTATTA 4701 TAGACTATAT GTTTTGAAGT TTGATATTAT AATGGGATAT TCATTTTTTG 4751 TTCTTTTCTT GACTCCTTTC TCAAGTGTGT GATAAGGTCT GCTGATAAAA 4801 TATTTAACCC CAAGAAAGTG AAAACTAATA TAAAATTAGA AAGACCTATC 4851 CAAATTAGAC AGTCAATTCC ATTAAAATAA GAAGTGAGAA AAACAATGTT 4901 GGGCATTGAG GTGTAAATTT TGCCCAGATG TATACCCAGT GTGAAATATC 4951 TTCTAATAAA AATATATTTG GCTCTTATCC CTGCACATGT AGAGGCATAA 5001 AAATTGGTAA ACATGTCCCG CTGTGTAGAA CTTTAAAAAA AAGGCATTTT 5051 TGAAAGTGTT GAGTGGCACT GATAACTGGT GAAGCCTACA GCCATCCGCC 5101 CAAAAGTCTG TTCTGATGGC ACTGAGTTTT CATTGTTCTG GATGTATAAG 5151 TCTGTGTGTC AGGTACAGCT GGGCCCAGCC AGCTTGAGTC ACTCTTGTAC 5201 AAGCTTGTTT TTTTCTGTCT TGTGAATGCA CTTGATAATT TAAAAATAAA 5251 AATATCTGTT TCTCTGCAAA AAAAAAA

BLAST Results

Entry HS32B1 from database EMBL:

Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone 32B1

Score = 4445, P = O.Oe+00, identities = 889/889

Entry U93816 from database EMBL:

Human exon-trapped sequence from 6q24.

Score = 965, P = 4.0e-35, identities = 193/193

Medline entries

No Medline entry

Peptide information for frame 1

ORF from 19 bp to 3606 bp; peptide length: 1196 Category: similarity to known protein 1 MPTAESEAKV KTKVRFEKLL KTHSDLMREK KKLKKKLVRS EENISPDTIR

51 SNLHYMKETT SDDPDTIRSN LPHIKETTSD DVSAANTNNL KKSTRVTKNK

101 LRNTQLATEN PNGDASVEED KQGKPNKKVI KTVPQLTTQD LKPETPENKV

151 DSTHQKTHTK PQPGVDHQKS EKANEGREET DLEEDEELMQ AYQCHVTEEM

201 AKEIKRKIRK KLKEQLTYFP SDTLFHDDKL SSEKRKKKKE VPVFSKAETS

251 TLTISGDTVE GEQKKESSVR SVSSDSHQDD EISSMEQSTE DSMQDDTKPK

301 PKKTKKKTKA VADNNEDVDG DGVHEITSRD SPVYPKCLLD DDLVLGVYIH

351 RTDRLKSDFM ISHPMVKIHV VDEHTGQYVK KDDSGRPVSS YYEKENVDYI

401 LPIMTQPYDF KQLKSRLPEW EEQIVFNENF PYLLRGSDES PKVILFFEIL

451 DFLSVDEIKN NSEVQNQECG FRKIAWAFLK LLGANGNANI NSKLRLQLYY

501 PPTKPRSPLS VVEAFEWWSK CPRNHYPSTL YVTVRGLKVP DCIKPSYRSM

551 MALQEEKGKP VHCERHHESS SVDTEPGLEE SKEVIKWKRL PGQACRIPNK

601 HLFSLNAGER GCFCLDFSHN GRILAAACAS RDGYPIILYE IPSGRFMREL

651 CGHLNIIYDL SWSKDDHYIL TSSSDGTARI WKNEINNTNT FRVLPHPSFV

701 YTAKFHPAVR ELVVTGCYDS MIRIWKVEMR EDSAILVRQF DVHKSFINSL

751 CFDTEGHHMY ΞGDCTGVIVV WNTYVKINDL EHSVHHWTIN KEIKETEFKG

801 IPISYLEIHP NGKRLLIHTK DSTLRIMDLR ILVARKFVGA ANYREKIHST

851 LTPCGTFLFA GSEDGIVYVW NPETGEQVAM YSDLPFKSPI RDISYHPFEN

901 MVAFCAFGQN EPILLYIYDF HVAQQEAEMF KRYNGTFPLP GIHQSQDALC

951 TCPKLPHQGS FQIDEFVHTE SSSTKMQLVK QRLETVTEVI RSCAAKVNKN

1001 LSFTSPPAVS SQQSKLKQSN MLTAQEILHQ FGFTQTGIIS IERKPCNHQV

1051 DTAPTVVALY DYTANRSDEL TIHRGDIIRV FFKDNEDWWY GSIGKGQEGY

1101 FPANHVASET LYQELPPEIK ERSPPLSPEE KTKIEKSPAP QKQSINKNKS

1151 QDFRLGSESM THSEMRKEQS HEDQGHIMDT RMRKNKQAGR KVTLIE

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_ln3, frame 1

TREMBL :U92792_1 gene: "tupl"; product: "Tupl"; Schizosaccharomyces pombe general transcriptional repressor Tupl (tupl) mRNA, complete eds., N = 1, Score = 186, P = le-10

TREMBL: AF104258_1 gene: "Pmc733"; product: "putative copper-mducible 35.6 kDa protein"; Festuca rubra putative copper-inducible 35.6 kDa protein (Pmc733) mRNA, complete eds., N = 1, Score = 235, P = 4.6e-18

TREMBL :SPAC3H5_8 gene: "SPAC3H5.08c"; product: "beta-transducm"; S. pombe chromosome I cosmid c3H5. , N = 2, Score = 231, P = 2e-14

PIR:T02533 hypothetical protein F13M22.17 - Arabidopsis thaliana, N = 2, Score = 228, P = le-13

TREMBL :AF104258_1 gene: "Pmc733"; product: "putative copper-inducible 35.6 kDa protein"; Festuca rubra putative copper-inducible 35.6 kDa protein (Pmc733) mRNA, complete eds., N = 1, Score = 235, P = 4.6e-18

TREMBL :SPAC3H5_8 gene: "SPAC3H5.08c"; product: "beta-transducin"; S. pombe chromosome I cosmid c3H5., N = 2, Score = 231, P = 2e-14

TREMBL :CER03E1_1 gene: "R03E1.1"; Caenorhabditis elegans cosmid R03E1, N = 1, Score = 215, P = 2.3e-13

SWISSPROT :YZLL_CAEEL HYPOTHETICAL 43.1 KD TRP-ASP REPEATS CONTAINING PROTEIN K04G11.4 IN CHROMOSOME X., N = 1, Score = 203, P = 7. le-13

>TREMBL:AF104258_1 gene: "Pmc733"; product: "putative copper-mducible 35.6 kDa protein"; Festuca rubra putative copper-mducible 35.6 kDa protein (Pmc733) mRNA, complete eds. Length = 321

HSPs:

Score = 235 (35.3 bits), Expect = 4.6e-18, P = 4.6e-18 Identities = 59/225 (26%), Positives = 111/225 (49%)

Query: 647 MRELCGHLNIIYDLSWSKDDHYILTSSSDGTARIWKNEINNTNTFRVLPHPSFVYTAKFH 706

+ E GH + I DLSWSK+ +L++S D T R+W ++ + +V H ++V +F+ Sbjct: 63 VHEFYGHGDAILDLSWSKNGD-LLSASMDKTVRLW—QVGRDSCLKVFSHTNYVTCVQFN 119

Query: 707 PAVRELVVTGCYDSMIRIWKVEMREDSAILVRQFDVHKSFINSLCFDTEGHHMYSGDCTG 766

P +TGC D ++RIW V LV + K + ++C+ +G +G TG Sbjct: 120 PTNGNYFITGCIDGLVRIWDVRK CLVVDWANSKEIVTAVCYRPDGKGAVAGTITG 174

Query: 767 VIVVWNTYVKINDLEHSVHHWTINKEIKETEFKGIPISYLEIHPNGKRLLIHTKDSTLRI 826 ++ +LE V ++N K + + Y P K+L++ + D+ +RI Sbjct: 175 NCRYYDASENRLELESQV SLNGRKKSLHKRIVGFQYCPSDP—KKLMVTSGDAQVRI 229 Query: 827 MDLRILVARKFVGAANYREKIHSTLTPCGTFLFAGSEDGIVYVWN 871 +D +++ + G + ++ + TP G + + S+D +Y+WN Sbjct: 230 LDGAHVISN-YKGLQS-SSQVARSFTPDGDHIVSASDDΞRIYMWN 272

Pedant information for DKFZphtes3_ln3, frame 1

Report for DKFZphtes3_ln3.1

[LENGTH] 1196

[MW] 137114.70

[pi] 6.79

[HOMOL] SWISSPROT :YKY4_CAEEL HYPOTHETICAL 40.4 KD TRP-ASP REPEATS CONTAINING PROTEIN

C14B1.4 IN CHROMOSOME III. 8e-21

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YKL121w] 2e-ll

[FUNCAT] 04.05.01.01 general transcription activities [S. cerevisiae, YBR198c

TAF90 - TFIID subunit] 4e-10

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YBR198C TAF90 - TFIID subunit]

4e-10

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YPR178w] le-08

[FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YPR178w] le-08

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YDR364c] 4e-08

[FUNCAT] 03.16 dna synthesis and replication [S. cerevisiae, YDR364c] 4e-08

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL145c]

9e-08

[FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae,

YDL145C] 9e-08

[FUNCAT] 04 . . 0 5.01.04 transcriptional control [S. cerevisiae, YCR084c] 2e-07

[FUNCAT] 10 . . 9 9 other signal-transduction activities [S. cerevisiae, YHL002w] 7e-07

[FUNCAT] 98 c.lassification not yet clear-cut [S. cerevisiae, YFR024c-a] 2e-06

[FUNCAT] 02 , . 1 6 fermentation [S. cerevisiae, YMR116c] 4e-06

[FUNCAT] 30 . . 0, 3 organization of cytoplasm [S. cerevisiae, YMR116c] 4e-06

[FUNCAT] 05 . . 0 4 translation (initiation, elongation and termination) [S. cerevisiae,

YMR116C ] 4e-06

[FUNCAT] 03 , . 1 0 sporulation and germination [S. cerevisiae, YFL009w] 4e-05

[FUNCAT] 03 , . 0 4 budding, cell polarity and filament formation [S. cerevisiae, YFL009w]

4e-05

[FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YFL009w] 4e-05

[FUNCAT] 03.01 cell growth [S. cerevisiae, YCR088w] 6e-05

[FUNCAT] 03.25 cytokinesis [S. cerevisiae, YCR057c] 7e-05

[BLOCKS] BL00024H

[SCOP] dltbgd_ 2.46 3.1.1 betal-subunit of the signal-transducmg 3e-91

[SCOP] dlgfc 2.21 2.1.9 Growth factor receptor-bound protein 2 (GRB2), N 4e-14

[SCOP] dlfmk_l 2.21 2.1.8 (1-64) c-src tyrosine kinase [human (Hom Se-15

[SCOP] dladδbl 2.21 2.1.7 (1-63) Hemapoetic cell kinase Hck [human (Hom 3e-15

[SCOP] dllckal 2.21 2.1.16 (1-54) p56-lck tyrosine kinase, SH3 domain [huma le-13

[SCOP] dlqwea_ 2.21 2.1.15 Src kinase, SH3 domain [Avian sarcoma virus 2e-15

[SCOP] dlshg_ 2.21 2.1.6 alpha-Spectnn, SH3 domain [chicken (Gallu 2e-13

[SCOP] dlprmc 2.21 2.1.13 Src kinase, SH3 domain [chicken (Gallus gallus) 2e-15

[SCOP] dlhsq_ 21.2.1.12 Phospholipase C, SH3 domain [human (Hom 2e-13

[SCOP] dlaboa 21.2.1.3 Abl tyrosine kinase, SH3 domain [Mouse (Mu 3e-13

[SCOP] dlefna 21.2.1.2 Fyn, SH3 domain [human (Homo sapiens) 2e-15

[SCOP] dlsema 21.2.1.11 Growth factor receptor-bound protein 2 (GRB2) , N le-13

[SCOP] dlgbqa 21.2.1.10 Growth factor receptor-bound protein 2 (GRB2), N 3e-16

[SCOP] dlckaa_ 21.2.1.1 C-Crk, N-terminal SH3 domain [mouse (Mu 3e-15

[EC] 3.1.4.3 Phospholipase C 2e-07

[EC] 3.1.4.11 l-Phosphatιdylιnosιtol-4, 5-bιsphosphate phosphodiesterase 7e-07

[EC] 3.6.1.32 Myosin ATPase 7e-07

[EC] 2 . 7 . 1 . 112 Protein-tyrosme kinase 8e-06

[PIRKW] nucleus 2e-08

[PIRKW] phosphotransferase 8e-06

[PIRKW] plasma 4e-07

[PIRKW] duplication 4e-07

[PIRKW] phosphoric diester hydrolase 2e-07

[PIRKW] tandem repeat 7e-07

[PIRKW] hormone 4e-07

[PIRKW] transmembrane protein 2e-06

[PIRKW] stomach 4e-07

[PIRKW] actm binding 7e-07

[PIRKW] ATP 7e-07

[PIRKW] phosphoprotem le-01

[PIRKW] signal transduction 7e-09

[PIRKW] heterotπmer 7e-09

[PIRKW] P-loop le-01

[PIRKW] hydrolase 7e-07

[PIRKW] transcription regulation 5e-06

[PIRKW] GTP binding 7e-09 [SUPFAM] l-phosphatιdylιnosιtol-4, 5-bisphosphate phosphodiesterase II 2e-07

[SUPFAM] SH3 homology 2e-07

[SUPFAM] SH2 homology 2e-07

[SUPFAM] protozoan myosin heavy chain IB 7e-07

[SUPFAM] myosin motor domain homology 7e-07

[SUPFAM] pleckstrm repeat homology 2e-07

[SUPFAM] protem-tyrosme kinase src 8e-06

[SUPFAM] WD repeat homology 3e-12

[SUPFAM] l-phosphatιdylιnosιtol-4, 5-bisphosphate phosphodiesterase domain Y homology 2e-

07

[SUPFAM] protein kinase homology 8e-06

[SUPFAM] l-phosphatιdylιnosιtol-4, 5-bisphosphate phosphodiesterase domain X homology 2e-

07

[SUPFAM] GTP-bindmg regulatory protein beta chain 7e-09

[SUPFAM] yeast coatomer complex alpha Cham 4e-07

[PROSITE] RGD 1

[PROSITE] MYRISTYL 6

[PROSITE] AMIDATION 2

[PROSITE] CAMP_PHOSPHO_SITE 4

[PROSITE] CK2_PHOSPHO_SITE 25

[PROSITE] TYR_PHOSPHO_SITE 4

[PROSITE] PKC_PHOSPHO_SITE 19

[PROSITE] ASN_GLYCOΞYLATION 6

[PFAM] Src homology domain 3

[PFAM] WD domain, G-beta repeats

[KW] Irregular

[KW] 3D

[KW] LOW_COMPLEXITY 5.77 %

[KW] COILED COIL 2.42 %

SEQ MPTAESEAKVKTKVRFEKLLKTHSDLMREKKKLKKKLVRSEENIΞPDTIRSNLHYMKETT SEG xxxxxxxx COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCC IgotB

SEQ SDDPDTIRSNLPHIKETTSDDVSAANTNNLKKSTRVTKNKLRNTQLATENPNGDASVEED SEG COILS IgotB

SEQ KQGKPNKKVIKTVPQLTTQDLKPETPENKVDSTHQKTHTKPQPGVDHQKSEKANEGREET SEG XXX COILS IgotB

SEQ DLEEDEELMQAYQCHVTEEMAKEIKRKIRKKLKEQLTYFPSDTLFHDDKLSSEKRKKKKE SEG XXXXXXXX XXXXXXXXXXXXXXX XXXXXXXXXXXX COILS IgotB

SEQ VPVFSKAETSTLTISGDTVEGEQKKESSVRSVΞSDSHQDDEISSMEQSTEDSMQDDTKPK SEG XXXXXXXXXX xxxx COILS IgotB

SEQ PKKTKKKTKAVADNNEDVDGDGVHEITSRDSPVYPKCLLDDDLVLGVYIHRTDRLKSDFM SEG xxxxxxxxx COILS IgotB

SEQ ISHPMVKIHVVDEHTGQYVKKDDSGRPVSSYYEKENVDYILPIMTQPYDFKQLKSRLPEW SEG COILS IgotB

SEQ EEQIVFNENFPYLLRGSDESPKVILFFEILDFLSVDEIKNNSEVQNQECGFRKIAWAFLK SEG COILS IgotB

SEQ LLGANGNANINΞKLRLQLYYPPTKPRSPLSVVEAFEWWSKCPRNHYPSTLYVTVRGLKVP SEG COILS IgotB

SEQ DCIKPSYRSMMALQEEKGKPVHCERHHESSSVDTEPGLEESKEVIKWKRLPGQACRIPNK SEG COILS IgotB SEQ HLFSLNAGERGCFCLDFSHNGRILAAACASRDGYPIILYEIPSGRFMRELCGHLNIIYDL SEG COILS IgotB CEEEEEECCCCCEEEE

SEQ SWSKDDHYILTSSSDGTARIWKNEINNTNTFRVLPHPSFVYTAKFHPAVRELVVTGCYDS SEG COILS IgotB EETTTTTEEEEEETTTEEEEEETT—TTCEEEEEETTTCEEEEEETTT-TCEEEEEETTT

SEQ MIRIWKVEMREDSAILVRQFDVHKSFINSLCFDTEGHHMYSGDCTGVIVVWNTYVKINDL SEG COILS IgotB EEEEEETTTTTBTTEEEEEEECCCCCE-EEEEEEETTEEEEEETTTEEEEEE

SEQ EHΞVHHWTINKEIKETEFKGIPISYLEIHPNGKRLLIHTKDSTLRIMDLRILVARKFVGA SEG COILS

IgotB

SEQ ANYREKIHSTLTPCGTFLFAGSEDGIVYVWNPETGEQVAMYSDLPFKSPIRDISYHPFEN SEG COILS IgotB

SEQ MVAFCAFGQNEPILLYIYDFHVAQQEAEMFKRYNGTFPLPGIHQSQDALCTCPKLPHQGS SEG COILS IgotB

SEQ FQIDEFVHTESSSTKMQLVKQRLETVTEVIRSCAAKVNKNLSFTSPPAVSSQQSKLKQSN SEG COILS IgotB

SEQ MLTAQEILHQFGFTQTGIISIERKPCNHQVDTAPTVVALYDYTANRSDELTIHRGDIIRV SEG COILS

IgotB

SEQ FFKDNEDWWYGSIGKGQEGYFPANHVASETLYQELPPEIKERSPPLSPEEKTKIEKSPAP SEG COILS IgotB

SEQ QKQSINKNKSQDFRLGSESMTHSEMRKEQSHEDQGHIMDTRMRKNKQAGRKVTLIE SEG COILS IgotB

Prosite for DKFZphtes3_ln3.1

PS00001 460->464 ASN_GLYCOSYLATION PDOC00001 PS00001 686->690 AΞN_GLYCOSYLATION PDOC00001 PS00001 934->938 ASN_GLYCOSYLATION PDOC00001 PS00001 1000->1004 ASN_GLYCOSYLATION PDOC00001 PS00001 1065->1069 ASN_GLYCOSYLATION PDOC00001 PS00001 1148->1152 ASN_GLYCOSYLATION PDOC00001 PS00004 91->95 CAMP_PHOSPHO_SITE PDOC00004 PS00004 264->268 CAMP_PHOSPHO_SITE PDOC00004 PS00004 305->309 CAMP_PHOSPHO_SITE PDOC00004 PS00004 1190->1194 CAMP_PHOSPHO_SITE PDOC00004 PS00005 48->51 PKC_PHOSPHO_SITE PDOC00005 PS00005 66->69 PKC_PHOSPHO_SITE PDOC00005 PS00005 93->96 PKC_PHOSPHO_SITE PDOC00005 PS00005 170->173 PKC_PHOSPHO_SITE PDOC00005 PS00005 232->235 PKC_PHOSPHO_SITE PDOC00005 PS00005 268->271 PKC_PHOSPHO_SITE PDOC00005 PS00005 304->307 PKC_PHOSPHO_SITE PDOC00005 PS00005 327->330 PKC_PHOSPHO_SITE PDOC00005 PS00005 352->355 PKC_PHOSPHO_SITE PDOC00005 PS00005 384->387 PKC_PHOSPHO_SITE PDOC00005 PS00005 440->443 PKC_PHOSPHO_SITE PDOC00005 PS00005 533->536 PKC_PHOSPHO_SITE PDOC00005 PS00005 546->549 PKC_PHOSPHO_SITE PDOC00005 PS00005 643->646 PKC_PHOSPHO_SITE PDOC00005 PS00005 677->680 PKC_PHOSPHO_SITE PDOC00005 PS00005 690->693 PKC_PHOSPHO_SITE PDOC00005 PS00005 702->705 PKC PHOSPHO SITE PDOC00005 PS00005 823->826 PKC PHOSPHO SITE PDOC00005

PS00005 973->976 PKC PHOSPHO^{" "}SITE PDOC00005

PS00006 22->26 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 59->63 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 77->81 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 116->120 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 137->141 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 180->184 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 245->249 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 276->280 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 283->287 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 288->292 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 292->296 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 327->331 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 390->394 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 454->458 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 510->514 CK2 PHOSPHO^{" "}SITE PDOC00006

PΞ00006 570->574 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 663->667 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 672->676 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 804->808 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 985->989 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 1023->1027 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 1127->1131 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 1132->1136 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 1161->1165 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 1170-M174 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00007 1083->1091 TYR PHOSPHO ^"SITE PDOC00007

PS00007 211->219 TYR PHOSPHO ^"SITE PDOC00007

PS00007 1083->1091 TYR PHOSPHO^{" "}SITE PDOC00007

PS00007 210->219 TYR PHOSPHO^{" "}SITE PDOC00007

PS00008 483->489 MYRISTYL PDOC00008

PS00008 577->583 MYRISTYL PDOC00008

PS00008 716->722 MYRISTYL PDOC00008

PS00008 800->806 MYRISTYL PDOC00008

PS00008 861->867 MYRISTYL PDOC00008

PS00008 941->947 MYRISTYL PDOC00008

PS00009 811->815 AMIDATION PDOC00009

PS00009 1188-M192 AMIDATION PDOC00009

PS00016 1074->1077 RGD PDOC00016

Pfam for DKFZphtes3_ln3.1

HMM_NAME WD domain, G-beta repeats

HMM *MrGHnnWVWCVaFSPDGrWFIvSGSWDgTCRLWD*

+ GH+N ++++++S D ++ I+++S DGT R+W Query 650 LCGHLNIIYDLSWSKDDHY-ILTSSSDGTARIWK 682

HMM_NAME Src homology domain 3

HMM *pyVIALYDYqAqdpDELSFkEGDIIiIIEdsDD.WWrgRnnnTNGQEGW

P+V+ALYDY+A+++DEL++ +GDII + ++++ WW+G GQEG+ Query 1054 PTVVALYDYTANRSDELTIHRGDIIRVFFKDNEDWWYGSIGK—GQEGY 1100

HMM IPSNYVEPi*

+P+N V+ + Query 1101 FPANHVASE 1109 DKFZphtes3_20c21

group: testes derived

DKFZphtes3_20c21 encodes a novel 708 ammo acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application m studying the expression profile of testis-specific genes . unknown

Sequenced by MediGenomix

Locus: /map="22qll.2-12.2"

Insert length: 3997 bp

Poly A stretch at pos. 3877, polyadenylation signal at pos. 3853

1 GGTAGGCGGG GCGGCGCGTG ACCTAAGGCC TCTCTGCCGC GCGCGCAGGT 51 ACGGGGCAGA AGTCGCAGGT ACCCAGCTGC TGCCCACGTT TCTGGTCCAG

101 AGTCCCGAAC CCCGAGCACT GGGATGCCTG GCTACTCCGA GCCAAGGCAC

151 TGATGTTTGA ACTGGAAACT TCAAAACGTT TAATAAGAGT CTTCAGGATG

201 GGTTTGAACT AGACAAGCTA GAAATTTCTT TAGAACACCA GCTCTAGCAT

251 GCATCTCCCA CTTTTGGCTT TCCTGGAGAG GAGCTTGAAG AGGTGGTTCT

301 GCAGACAGCC ACAGTGATAC TCAGGAAACC AGAGGAATGG ATTTGACTTT

351 TCTGCTAGGA TTCTTTGTTA TAGTTTCTCC CTGAGTTGTA AGAGGCATGG

401 AAATATACAT GAAACTGAAG AACCTGCAAG GAAGGGAAGT GGAACTTTCC

451 ATGCTGAGTG AAAACTAACC AAGTGGCAGT TGTGACTGAA AACACTGAAA

501 CCTACCACGT CCAGATTCAC TGGATTGGGG GATAGAGGAA CGGTCACAGC

551 TAGGGAGAAA GAAGTGATAC CGGAAAAGAA AACCTAAATG AAGAGAATGA

601 GGATGACTGC ACAGTAGATG GCCACCTCTA CCTCCACAGA GGCAAAGTCA

651 GCCTCGTGGT GGAATTATTT TTTTCTTTAT GATGGTTCCA AGGTAAAGGA

701 AGAAGGCGAT CCAACAAGAG CTGGCATTTG TTACTTTTAT CCTTCCCAGA

751 CCCTGCTAGA CCAACAGGAG TTGCTTTGTG GACAGATTGC TGGAGTTGTC

801 CGCTGTGTTT CTGACATTTC TGACTCTCCT CCTACTCTTG TTCGTCTGAG

851 AAAACTGAAG TTTGCCATAA AAGTTGATGG AGATTACCTT TGGGTGCTGG

901 GCTGTGCTGT GGAGCTCCCT GATGTCAGCT GCAAGCGGTT TCTGGATCAG

951 CTAGTTGGAT TCTTTAATTT TTACAATGGA CCTGTTTCCC TAGCTTATGA 1001 GAACTGTTCT CAGGAAGAAC TGAGCACGGA GTGGGACACC TTCATCGAGC 1051 AAATTCTGAA AAACACCAGT GATCTGCATA AGATTTTCAA TTCCCTCTGG 1101 AACTTGGACC AAACTAAAGT GGAGCCCCTG TTGTTGCTGA AGGCAGCCCG 1151 CATTCTGCAG ACCTGCCAGC GCTCGCCTCA CATTCTCGCT GGCTGCATCC 1201 TCTATAAAGG ACTGATTGTC AGCACCCAAC TCCCGCCCTC CCTCACCGCC 1251 AAGGTCCTGC TTCACCGAAC AGCACCTCAG GAGCAGAGAC TCCCTACGGG 1301 AGGGGATGCC CCGCAGGAAC ATGGAGCGGC ATTGCCCCCG AATGTCCAGA 1351 TTATCCCTGT TTTTGTGACC AAAGAGGAAG CCATTAGTCT CCACGAGTTC 1401 CCGGTGGAAC AGATGACAAG GTCTCTAGCA TCTCCAGCAG GACTCCAGGA 1451 TGGTTCAGCC CAGCACCATC CAAAGGGTGG GAGCACATCT GCCCTGAAAG 1501 AAAACGCCAC TGGCCATGTG GAATCCATGG CCTGGACCAC CCCAGATCCC 1551 ACATCCCCTG ACGAAGCTTG TCCAGATGGC AGGAAGGAGA ACGGATGCTT 1601 GTCTGGCCAT GATCTGGAGA GCATCAGGCC CGCAGGACTG CACAACTCTG 1651 CCAGGGGTGA GGTTCTTGGC CTCAGCTCCT CCCTGGGGAA GGAACTAGTC 1701 TTTCTCCAAG AAGAACTCGA CTTGTCTGAA ATCCACATTC CAGAGGCTCA 1751 GGAAGTGGAA ATGGCCTCAG GTCATTTTGC CTTCCTACAT GTGCCTGTTC 1801 CAGATGGCAG GGCTCCTTAC TGCAAGGCAT CTCTCAGCGC CTCCAGCAGC 1851 CTGGAACCCA CGCCTCCTGA GGACACAGCC ATCAGCAGCT TGCGCCCTCC 1901 CTCTGCTCCT GAGATGCTGA CCCAGCATGG AGCCCAAGAG CAGGTCGAAG 1951 ACCATCCTGG CCATAGCAGC CAAGCCCCCA TTCCCAGAGC AGACCCTCTC 2001 CCCAGAAGGA CCCGCAGGCC CTTGTTATTG CCTCGCTTAG ATCCAGGACA 2051 GAGAGGAAAC AAGCTTCCCA CGGGGGAACA AGGCCTGGAT GAGGATGTTG 2101 ATGGGGTCTG TGAAAGCCAC GCAGCCCCTG GTCTGGAATG CAGTTCAGGC 2151 TCAGCAAACT GTCAGGGTGC TGGCCCCTCT GCAGATGGAA TCAGCTCCAG 2201 GCTGACACCA GCAGAGTCCT GCATGGGGCT CGTGAGGATG AATCTCTACA 2251 CTCACTGCGT CAAAGGGCTG ATGCTGTCCC TGCTGGCTGA GGAGCCGCTG 2301 CTGGGAGACA GCGCAGCCAT AGAGGAAGTG TACCACAGCA GCCTGGCTTC 2351 ACTGAATGGG CTGGAAGTCC ACCTGAAAGA GACGCTGCCC AGGGATGAGG 2401 CAGCCTCCAC GAGCAGCACC TACAACTTCA CATATTACGA CCGCATTCAG 2451 AGCTTGCTGA TGGCAAACCT GCCGCAGGTG GCCACCCCGC ATGATCGCCG 2501 CTTCCTCCAG GCCGTCAGCC TGATGCATAG CGAATTTGCC CAGCTGCCCG 2551 CGCTTTATGA AATGACTGTC AGAAATGCCT CCACGGCTGT GTACGCCTGT 2601 TGCAACCCCA TCCAGGAGAC ATATTTCCAG CAGCTGGCAC CTGCAGCACG 2651 GAGCTCCGGC TTCCCAAACC CTCAGGATGG CGCCTTCAGC CTCTCCGGCA 2701 AAGCAAAGCA GAAGCTGCTG AAGCACGGGG TGAACTTGCT CTGAACTGCA 2751 CCCAGGAGGT GACTGGGAAG GAGAAAACCA GCAAAGGAAG CTCTGCCTTT 2801 TATAATTGAA AAGGCCCCTC TATTTTATTT TTCTTGAAAA CATTCCCTTT 2851 TTTAGGAACC AAATGATATT TGAGTTTTTG TTATTCCTTT TGCAGATTGG

2901 GATGTGTTTT GGGGGCAGGG GTTAGTTCTT CAGGTCGGCA GACCCAGAGC

2951 ACTTGATAAA GAACTGTATT TAATCGGTAG TGTTGGGGCC GGGACGGGCT

3001 TGGCTCCCTC TCTGCCATAC TGAGCCTGAG GTATTTCATA TCTCCTGCTG

3051 TTCCATCCCA GCTTGAATTG GTGCCACAAG CTTCCAAGTT GGCATTTTTT

3101 CTAGAACCTG ATCGTCCACT AGCCCAGAGT GTGTGTGTTC AACCCCCACA

3151 CCAGGTGGTG GTAGGCGGTG TGACTGCACA GCGAGGTGCC GGATCTGTGA

3201 GCAGGCCGAC TCCACTCCCA CGCCGCAGGT AGGTTTCTCC AGTGCGCTCT

3251 TGCTGGGAGG TCCGGATCGT TCCTGCAGGG AAGCGGCAGC ACACGGAGAC

3301 CACTTGGTTG AATTCTGTTG GAACTCTACT CAAATCTAGG GGCGTCTTCT

3351 TTGGACCCAC AATGGGGGCA AGCCTTAATA ATATGGAAGG GAGTTTGGGC

3401 TTTAGAGATC CCTTTATAAA AGCTCTGGGG GCTGAGCCCT GAGAATTCAG

3451 TGACAACAGG ACCAACCTGC GCTGCCTTTG ACTACAAGTG GGCCGTGCAG

3501 CTGGTTCCTC TCGAGCGAGT GTCCCTAAAT AGGAGTTTAC AAGATGTCTG

3551 GGGGTAAAAG CACTGTGCTT TTCAGTGGTG GCTGCGTGAA AGGGAGCGAC

3601 ACTCAGCTGT GTGTTCCTGG GCTTGTGTGG TACTTAGAAC CTCAGTTCTA

3651 TTACGTTATA GTCAGACATT TTTTTGACAG TATGAGACAG ACTGCAGGAT

3701 GAAAATATTT GTCAAAATCT TAACTGAATG TTTACTGGAA GTACTTGAGA

3751 TTCCATTTGA GAGTTGTATT GTTAATAATT TCATGTCAGT GAACTGATAT

3801 CTGATGTTTA TGATATGGTG TCTTTTTCTT GAAACAAGCT TCCAAGGGCT

3851 AGAAATAAAA TAGCCAAAAA ATGCTGGAAA AAAAAAAAAA AAAAAAAAAA

3901 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

3951 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAA

BLAST Results

Entry HS1048E9 from database EMBLNEW:

Human DNA sequence from clone 1048E9 on chromosome 22qll.2-12.2

Contains pseudogene similar to ribosomal protein S3A and part of a gene similar to C. elegans protein CE02118, ESTs, STS, GΞΞ.

Score = 6540, P = O.Oe+00, identities = 1308/1308

-14 exons

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 618 bp to 2741 bp; peptide length: 708 Category: putative protein Classification: no clue

1 MATSTSTEAK SASWWNYFFL YDGSKVKEEG DPTRAGICYF YPSQTLLDQQ

51 ELLCGQIAGV VRCVSDISDS PPTLVRLRKL KFAIKVDGDY LWVLGCAVEL

101 PDVSCKRFLD QLVGFFNFYN GPVSLAYENC SQEELSTEWD TFIEQILKNT

151 SDLHKIFNSL WNLDQTKVEP LLLLKAARIL QTCQRSPHIL AGCILYKGLI

201 VSTQLPPSLT AKVLLHRTAP QEQRLPTGGD APQEHGAALP PNVQIIPVFV

251 TKEEAISLHE FPVEQMTRSL ASPAGLQDGS AQHHPKGGST ΞALKENATGH

301 VESMAWTTPD PTSPDEACPD GRKENGCLSG HDLESIRPAG LHNSARGEVL

351 GLSSSLGKEL VFLQEELDLS EIHIPEAQEV EMASGHFAFL HVPVPDGRAP

401 YCKASLSASS SLEPTPPEDT AISSLRPPSA PEMLTQHGAQ EQVEDHPGHS

451 SQAPIPRADP LPRRTRRPLL LPRLDPGQRG NKLPTGEQGL DEDVDGVCES

501 HAAPGLECSS GSANCQGAGP SADGISSRLT PAEΞCMGLVR MNLYTHCVKG

551 LMLSLLAEEP LLGDSAAIEE VYHSSLASLN GLEVHLKETL PRDEAASTSS

601 TYNFTYYDRI QSLLMANLPQ VATPHDRRFL QAVSLMHSEF AQLPALYEMT

651 VRNASTAVYA CCNPIQETYF QQLAPAARΞS GFPNPQDGAF SLSGKAKQKL

701 LKHGVNLL

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_20c21, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphtes3 20c21, frame 3

Report for DKFZphtes3 20c21.3 [ LENGTH ] 708

[MW] 76900 . 23

[pi] 5.30

[KW] Alpha Beta

[KW] LOW COMPLEXITY 6 . 36 %

SEQ MATSTSTEAKSASWWNYFFLYDGSKVKEEGDPTRAGICYFYPSQTLLDQQELLCGQIAGV

SEG . xxxxxxxxxxxx

PRD ccccccccccccccceeeeeccccccccccccccccceeeeccchhhhhhhhhhhcccee

SEQ VRCVSDISDSPPTLVRLRKLKFAIKVDGDYLWVLGCAVELPDVSCKRFLDQLVGFFNFYN

SEG

PRD eeeeeeccccccchhhhhhhhheeeeccceeeeeeeeeecccccchhhhhhhhheeeecc

SEQ GPVSLAYENCSQEELSTEWDTFIEQILKNTSDLHKIFNSLWNLDQTKVEPLLLLKAARIL

SEG

PRD ccccccccccchhhhhhhhhhhhhhhhhhcchhhhhhhcccccccccchhhhhhhhhhhh

SEQ QTCQRSPHILAGCILYKGLIVSTQLPPSLTAKVLLHRTAPQEQRLPTGGDAPQEHGAALP

SEG

PRD hhhhccccchhhhhhhcccccccccccchhhhhhhhhccccccccccccccccccccccc

SEQ PNVQIIPVFVTKEEAISLHEFPVEQMTRSLASPAGLQDGSAQHHPKGGSTSALKENATGH

SEG

PRD ccceeeeeeeecccceeeccccchhhhhhhccccccccccccccccccchhhhhhhcccc

SEQ VESMAWTTPDPTSPDEACPDGRKENGCLSGHDLESIRPAGLHNSARGEVLGLSΞSLGKEL

SEG

PRD ccccccccccccccccccccccccccccccccccccccccccccccceeeeeccccchhh

SEQ VFLQEELDLΞEIHIPEAQEVEMASGHFAFLHVPVPDGRAPYCKASLSASSSLEPTPPEDT

SEG

PRD hhhhhhhcccccccccchhhhhhccceeeeeecccccccceeeccccccccccccccccc

SEQ AISSLRPPSAPEMLTQHGAQEQVEDHPGHSSQAPIPRADPLPRRTRRPLLLPRLDPGQRG

SEG xxxxxxxxxxxxxxxxxxxxx ....

PRD cccccccccchhhhhhccccceeecccccccccccccccccccccccccccccccccccc

SEQ NKLPTGEQGLDEDVDGVCESHAAPGLECSSGSANCQGAGPSADGISSRLTPAESCMGLVR

SEG

PRD ccccccccccccccccccccccccccccccccccccccccccccccccccccccceeeee

SEQ MNLYTHCVKGLMLSLLAEEPLLGDSAAIEEVYHSSLASLNGLEVHLKETLPRDEAASTSS

SEG xxxxxxxxxxxx

PRD ceeeeeeehhhhhhhhhccccccchhhhhhhhhhccccccchhhhhhhcccccccccccc

SEQ TYNFTYYDRIQSLLMANLPQVATPHDRRFLQAVSLMHSEFAQLPALYEMTVRNAΞTAVYA

SEG

PRD ccceeeehhhhhhhhhcccccccccchhhhhhhhhhhhhhhcchhhhhhhhhccceeeee

SEQ CCNPIQETYFQQLAPAARSSGFPNPQDGAFSLSGKAKQKLLKHGVNLL

SEG

PRD eccchhhhhhhhhhhhhhhcccccccccceeecchhhhhhhhhccccc

(No Prosite data available for DKFZphtes3_20c21.3) (No Pfam data available for DKFZphtes3_20c21.3)

DKFZphtes3_20k2

group: signal transduction

DKFZphtes3_20k2 encodes a novel 839 amino acid protein with strong similarity to rat vamlloid receptor subtype 1.

VRl seems to play an important role m the activation and sensitization of nociceptors. It is the receptor for e.g. capsaicin, a selective activator of nociceptors, a natural product of capsicum peppers. The novel protein is the human orthologue of rat VRl.

The new protein can find application as a target for the development of new nociception- modulating drugs . strong similarity to rat vamlloid receptor subtype 1

Sequenced by MediGenomix

Locus : unknown

Insert length: 4187 bp

Poly A stretch at pos. 4154, polyadenylation signal at pos. 4135

1 GGCTCAGGCA GGCCTGGCCC AGAGTCACGC TGGCAACCAC GAGTTTGGGA 51 AGCAGTCGTA TTCTCTCTCT CTCTCTCTCT CTCTCAGTAT CCATGACAGT

101 GTGATGGAGA GTCTCTGCCG TGCCATCTGG GATGCAAACC GTCCCTGTGT

151 CCCCCACGTC CAGGCCGTAG ATGCTCCCCG CCGGTCAGTC ACTTAGTCGT

201 CAGATCGCCC GTCCTGGTAT CACAGTGCTT CTGTTCAGGT TGCACACTGG

251 GCCACAGAGG ATCCAGCAAG GATGAAGAAA TGGAGCAGCA CAGACTTGGG

301 GGCAGCTGCG GACCCACTCC AAAAGGACAC CTGCCCAGAC CCCCTGGATG

351 GAGACCCTAA CTCCAGGCCA CCTCCAGCCA AGCCCCAGCT CTCCACGGCC

401 AAGAGCCGCA CCCGGCTCTT TGGGAAGGGT GACTCGGAGG AGGCTTTCCC

451 GGTGGATTGC CCTCACGAGG AAGGTGAGCT GGACTCCTGC CCGACCATCA

501 CAGTCAGCCC TGTTATCACC ATCCAGAGGC CAGGAGACGG CCCCACCGGT

551 GCCAGGCTGC TGTCCCAGGA CTCTGTCGCC GCCAGCACCG AGAAGACCCT

601 CAGGCTCTAT GATCGCAGGA GTATCTTTGA AGCCGTTGCT CAGAATAACT

651 GCCAGGATCT GGAGAGCCTG CTGCTCTTCC TGCAGAAGAG CAAGAAGCAC

701 CTCACAGACA ACGAGTTCAA AGACCCTGAG ACAGGGAAGA CCTGTCTGCT

751 GAAAGCCATG CTCAACCTGC ATGACGGACA GAACACCACC ATCCCCCTGC

801 TCCTGGAGAT CGCGCGGCAA ACGGACAGCC TGAAGGAGCT TGTCAACGCC

851 AGCTACACGG ACAGCTACTA CAAGGGCCAG ACAGCACTGC ACATCGCCAT

901 CGAGAGACGC AACATGGCCC TGGTGACCCT CCTGGTGGAG AACGGAGCAG

951 ACGTCCAGGC TGCGGCCCAT GGGGACTTCT TTAAGAAAAC CAAAGGGCGG 1001 CCTGGATTCT ACTTCGGTGA ACTGCCCCTG TCCCTGGCCG CGTGCACCAA 1051 CCAGCTGGGC ATCGTGAAGT TCCTGCTGCA GAACTCCTGG CAGACGGCCG 1101 ACATCAGCGC CAGGGACTCG GTGGGCAACA CGGTGCTGCA CGCCCTGGTG 1151 GAGGTGGCCG ACAACACGGC CGACAACACG AAGTTTGTGA CGAGCATGTA 1201 CAATGAGATT CTGATCCTGG GGGCCAAACT GCACCCGACG CTGAAGCTGG 1251 AGGAGCTCAC CAACAAGAAG GGAATGACGC CGCTGGCTCT GGCAGCTGGG 1301 ACCGGGAAGA TCGGGGTCTT GGCCTATATT CTCCAGCGGG AGATCCAGGA 1351 GCCCGAGTGC AGGCACCTGT CCAGGAAGTT CACCGAGTGG GCCTACGGGC 1401 CCGTGCACTC CTCGCTGTAC GACCTGTCCT GCATCGACAC CTGCGAGAAG 1451 AACTCGGTGC TGGAGGTGAT CGCCTACAGC AGCAGCGAGA CCCCTAATCG 1501 CCACGACATG CTCTTGGTGG AGCCGCTGAA CCGACTCCTG CAGGACAAGT 1551 GGGACAGATT CGTCAAGCGC ATCTTCTACT TCAACTTCCT GGTCTACTGC 1601 CTGTACATGA TCATCTTCAC CATGGCTGCC TACTACAGGC CCGTGGATGG 1651 CTTGCCTCCC TTTAAGATGG AAAAAATTGG AGACTATTTC CGAGTTACTG 1701 GAGAGATCCT GTCTGTGTTA GGAGGAGTCT ACTTCTTTTT CCGAGGGATT 1751 CAGTATTTCC TGCAGAGGCG GCCGTCGATG AAGACCCTGT TTGTGGACAG 1801 CTACAGTGAG ATGCTTTTCT TTCTGCAGTC ACTGTTCATG CTGGCCACCG 1851 TGGTGCTGTA CTTCAGCCAC CTCAAGGAGT ATGTGGCTTC CATGGTATTC 1901 TCCCTGGCCT TGGGCTGGAC CAACATGCTC TACTACACCC GCGGTTTCCA 1951 GCAGATGGGC ATCTATGCCG TCATGATAGA GAAGATGATC CTGAGAGACC 2001 TGTGCCGTTT CATGTTTGTC TACATCGTCT TCTTGTTCGG GTTTTCCACA 2051 GCGGTGGTGA CGCTGATTGA AGACGGGAAG AATGACTCCC TGCCGTCTGA 2101 GTCCACGTCG CACAGGTGGC GGGGGCCTGC CTGCAGGCCC CCCGATAGCT 2151 CCTACAACAG CCTGTACTCC ACCTGCCTGG AGCTGTTCAA GTTCACCATC 2201 GGCATGGGCG ACCTGGAGTT CACTGAGAAC TATGACTTCA AGGCTGTCTT 2251 CATCATCCTG CTGCTGGCCT ATGTAATTCT CACCTACATC CTCCTGCTCA 2301 ACATGCTCAT CGCCCTCATG GGTGAGACTG TCAACAAGAT CGCACAGGAG 2351 AGCAAGAACA TCTGGAAGCT GCAGAGAGCC ATCACCATCC TGGACACGGA 2401 GAAGAGCTTC CTTAAGTGCA TGAGGAAGGC CTTCCGCTCA GGCAAGCTGC 2451 TGCAGGTGGG GTACACACCT GATGGCAAGG ACGACTACCG GTGGTGCTTC 2501 AGGGTGGACG AGGTGAACTG GACCACCTGG AACACCAACG TGGGCATCAT 2551 CAACGAAGAC CCGGGCAACT GTGAGGGCGT CAAGCGCACC CTGAGCTTCT 2601 CCCTGCGGTC AAGCAGAGTT TCAGGCAGAC ACTGGAAGAA CTTTGCCCTG 2651 GTCCCCCTTT TAAGAGAGGC AAGTGCTCGA GATAGGCAGT CTGCTCAGCC 2701 CGAGGAAGTT TATCTGCGAC AGTTTTCAGG GTCTCTGAAG CCAGAGGACG

2751 CTGAGGTCTT CAAGAGTCCT GCCGCTTCCG GGGAGAAGTG AGGACGTCAC

2801 GCAGACAGCA CTGTCAACAC TGGGCCTTAG GAGACCCCGT TGCCACGGGG

2851 GGCTGCTGAG GGAACACCAG TGCTCTGTCA GCAGCCTGGC CTGGTCTGTG

2901 CCTGCCCAGC ATGTTCCCAA ATCTGTGCTG GACAAGCTGT GGGAAGCGTT

2951 CTTGGAAGCA TGGGGAGTGA TGTACATCCA ACCGTCACTG TCCCCAAGTG

3001 AATCTCCTAA CAGACTTTCA GGTTTTTACT CACTTTACTA AACAGTTTGG

3051 ATGGTCAGTC TCTACTGGGA CATGTTAGGC CCTTGTTTTC TTTGATTTTA

3101 TTCTTTTTTT TGAGACAGAA TTTCACTCTT CTCACCCAGG CTGGAATGCA

3151 GTGGCACAAT TTTGGCTCCC TGCAACCTCC GCCTCCTGGA TTCCAGCAAT

3201 TCTCCTGCCT CGGCTTCCCA AGTAGCTGGG ATTACAGGCA CGTGCCACCA

3251 TGTCTGGCTA ATTTTTTGTA TTTTTTTAAT AGATATGGGG TTTCGCCATG

3301 TTGGCCAGGC TGGTCTCGAA CTCCTGACCT CAGGTGATCC GCCCACCTCG

3351 GCCTCCCAAA GTGCTGGGAT TACAGGTGTG AGCCTCCACA CCTGGCTGTT

3401 TTCTTTGATT TTATTCTTTT TTTTTTTTCT GTGAGACAGA GTTTCACTCT

3451 TGTTGCCCAG GCTGGAGTGC AGTGGTGTGA TCTTGGCTCA CTGCAACCTC

3501 TGCCTCCCGG GTTCAAGCGA TTCTTCTGCT TCAGTCTCCC AAGTAGCTTG

3551 GATTACAGGT GAGCACTACC ACGCCCGGCT AATTTTTGTA TTTTTAATAG

3601 AGACGGGGTT TCACCATGTT GGCCAGGCTG GTCTCGAACT CTTGACCTCA

3651 GGTGATCTGC CCGCCTTGGC CTCCCAAAGT GCTGGGATTA CAGGTGTGAG

3701 CCGCTGCGCT CGGCCTTCTT TGATTTTATA TTATTAGGAG CAAAAGTAAA

3751 TGAAGCCCAG GAAAACACCT TTGGGAACAA ACTCTTCCTT TGATGGAAAA

3801 TGCAGAGGCC CTTCCTCTCT GTGCCGTGCT TGCTCCTCTT ACCTGCCCGG

3851 GTGGTTTGGG GGTGTTGGTG TTTCCTCCCT GGAGAAGATG GGGGAGGCTG

3901 TCCCACTCCC AGCTCTGGCA GAATCAAGCT GTTGCAGCAG TGCCTTCTTC

3951 ATCCTTCCTT ACGATCAATC ACAGTCTCCA GAAGATCAGC TCAATTGCTG

4001 TGCAGGTTAA AACTACAGAA CCACATCCCA AAGGTACCTG GTAAGAATGT

4051 TTGAAAGATC TTCCATTTCT AGGAACCCCA GTCCTGCTTC TCCGCAATGG

4101 CACATGCTTC CACTCCATCC ATACTGGCAT CCTCAAATAA ACAGATATGT

4151 ATACATATAA AAAAAAAAAA AAAAAAAAAA AAAAAAA

BLAST Results

No BLAST result

Medline entries

99288727:

Recent advances in neuropharmacology of cutaneous nociceptors.

99231880:

A non-pungent triprenyl phenol of fungal origin, scutigeral, stimulates rat dorsal root ganglion neurons via interaction at vamlloid receptors .

Peptide information for frame 2

ORF from 272 bp to 2788 bp; peptide length: 839 Category: strong similarity to known protein Classification: Cell signaling/communication

1 MKKWSSTDLG AAADPLQKDT CPDPLDGDPN SRPPPAKPQL STAKSRTRLF

51 GKGDSEEAFP VDCPHEEGEL DSCPTITVSP VITIQRPGDG PTGARLLSQD

101 SVAASTEKTL RLYDRRSIFE AVAQNNCQDL ESLLLFLQKS KKHLTDNEFK

151 DPETGKTCLL KAMLNLHDGQ NTTIPLLLEI ARQTDSLKEL VNASYTDSYY

201 KGQTALHIAI ERRNMALVTL LVENGADVQA AAHGDFFKKT KGRPGFYFGE

251 LPLSLAACTN QLGIVKFLLQ NSWQTADISA RDSVGNTVLH ALVEVADNTA

301 DNTKFVTSMY NEILILGAKL HPTLKLEELT NKKGMTPLAL AAGTGKIGVL

351 AYILQREIQE PECRHLSRKF TEWAYGPVHS SLYDLSCIDT CEKNΞVLEVI

401 AYSSSETPNR HDMLLVEPLN RLLQDKWDRF VKRIFYFNFL VYCLYMIIFT

451 MAAYYRPVDG LPPFKMEKIG DYFRVTGEIL SVLGGVYFFF RGIQYFLQRR

501 PΞMKTLFVDΞ YSEMLFFLQS LFMLATVVLY FSHLKEYVAS MVFSLALGWT

551 NMLYYTRGFQ QMGIYAVMIE KMILRDLCRF MFVYIVFLFG FSTAVVTLIE

601 DGKNDSLPSE STSHRWRGPA CRPPDSSYNS LYSTCLELFK FTIGMGDLEF

651 TENYDFKAVF IILLLAYVIL TYILLLNMLI ALMGETVNKI AQESKNIWKL

701 QRAITILDTE KSFLKCMRKA FRSGKLLQVG YTPDGKDDYR WCFRVDEVNW

751 TTWNTNVGII NEDPGNCEGV KRTLSFSLRΞ SRVSGRHWKN FALVPLLREA

801 SARDRQSAQP EEVYLRQFSG ΞLKPEDAEVF KSPAASGEK

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_20k2, frame 2

TREMBL:AF029310_1 product: "vamlloid receptor subtype 1"; Rattus norvegicus vamlloid receptor subtype 1 mRNA, complete eds., N = 1, Score = 3760, P = 0

TREMBLNEW:AB015231_1 product: "stretch-inhibitable nonselective channel (SIC)"; Rattus norvegicus mRNA for stretch-inhibitable nonselective channel (SIC), complete eds., N = 2, Score = 2090, P = 2e-219

>TREMBL:AF029310_1 product: "vamlloid receptor subtype 1"; Rattus norvegicus vamlloid receptor subtype 1 mRNA, complete eds. Length = 838

HSPs:

Score = 3760 (564.1 bits), Expect = O.Oe+00, P = O.Oe+00 Identities = 721/839 (85%), Positives = 773/839 (92%)

Query: 1 MKKWSSTDLGAAADPLQKDTCPDPLDGDPNSRPPPAKPQLSTAKSRTRLFGKGDSEEAFP 60

M++ +S D + P Q+++C DP D DPN +PPP KP + T +SRTRLFGKGDSEEA P Sbjct: 1 MEQRASLDSEESESPPQENSCLDPPDRDPNCKPPPVKPHIFTTRSRTRLFGKGDSEEASP 60

Query: 61 VDCPHEEGELDSCPTITVSPVITIQRPGDGPTGARLLSQDSVAASTEKTLRLYDRRSIFE 120

+DCP+EEG L SCP ITVS V+TIQRPGDGP R SQDSV+A EK RLYDRRSIF+ Sbjct: 61 LDCPYEEGGLASCPIITVSSVLTIQRPGDGPASVRPSSQDSVSAG-EKPPRLYDRRSIFD 119

Query: 121 AVAQNNCQDLESLLLFLQKΞKKHLTDNEFKDPETGKTCLLKAMLNLHDGQNTTIPLLLEI 180

AVAQ+NCQ+LESLL FLQ+SKK LTD+EFKDPETGKTCLLKAMLNLH+GQN TI LLL++ Sbjct: 120 AVAQSNCQELESLLPFLQRSKKRLTDSEFKDPETGKTCLLKAMLNLHNGQNDTIALLLDV 179

Query: 181 ARQTDSLKELVNASYTDSYYKGQTALHIAIERRNMALVTLLVENGADVQAAAHGDFFKKT 240

AR+TDSLK+ VNASYTDSYYKGQTALHIAIERRNM LVTLLVENGADVQAAA+GDFFKKT Sbjct: 180 ARKTDSLKQFVNASYTDSYYKGQTALHIAIERRNMTLVTLLVENGADVQAAANGDFFKKT 239

Query: 241 KGRPGFYFGELPLSLAACTNQLGIVKFLLQNSWQTADISARDSVGNTVLHALVEVADNTA 300

KGRPGFYFGELPLSLAACTNQL IVKFLLQNSWQ ADISARDSVGNTVLHALVEVADNT Sb_jct: 240 KGRPGFYFGELPLSLAACTNQLAIVKFLLQNSWQPADIΞARDSVGNTVLHALVEVADNTV 299

Query: 301 DNTKFVTSMYNEILILGAKLHPTLKLEELTNKKGMTPLALAAGTGKIGVLAYILQREIQE 360

DNTKFVTSMYNEILILGAKLHPTLKLEE+TN+KG+TPLALAA +GKIGVLAYILQREI E Sbjct: 300 DNTKFVTSMYNEILILGAKLHPTLKLEEITNRKGLTPLALAASSGKIGVLAYILQREIHE 359

Query: 361 PECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSSETPNRHDMLLVEPLN 420

PECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSSETPNRHDMLLVEPLN Sbjct: 360 PECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSSETPNRHDMLLVEPLN 419

Query: 421 RLLQDKWDRFVKRIFYFNFLVYCLYMIIFTMAAYYRPVDGLPPFKMEK-IGDYFRVTGEI 479

RLLQDKWDRFVKRIFYFNF VYCLYMIIFT AAYYRPV+GLPP+K++ +GDYFRVTGEI Sbjct: 420 RLLQDKWDRFVKRIFYFNFFVYCLYMIIFTAAAYYRPVEGLPPYKLKNTVGDYFRVTGEI 479

Query: 480 LSVLGGVYFFFRGIQYFLQRRPSMKTLFVDSYSEMLFFLQSLFMLATVVLYFSHLKEYVA 539

LSV GGVYFFFRGIQYFLQRRPS+K+LFVDSYSE+LFF+QSLFML +VVLYFS KEYVA Sbjct: 480 LSVSGGVYFFFRGIQYFLQRRPSLKSLFVDSYΞEILFFVQSLFMLVSVVLYFSQRKEYVA 539

Query: 540 SMVFSLALGWTNMLYYTRGFQQMGIYAVMIEKMILRDLCRFMFVYIVFLFGFSTAVVTLI 599

SMVFSLA+GWTNMLYYTRGFQQMGIYAVMIEKMILRDLCRFMFVY+VFLFGFSTAVVTLI Sbjct: 540 SMVFSLAMGWTNMLYYTRGFQQMGIYAVMIEKMILRDLCRFMFVYLVFLFGFSTAVVTLI 599

Query: 600 EDGKNDSLPSESTSHRWRGPACRPPDSSYNSLYSTCLELFKFTIGMGDLEFTENYDFKAV 659

EDGKN+SLP EST H+ RG AC+P +SYNSLYSTCLELFKFTIGMGDLEFTENYDFKAV Sb_jct: 600 EDGKNNSLPMESTPHKCRGSACKP-GNSYNSLYSTCLELFKFTIGMGDLEFTENYDFKAV 658

Query: 660 FIILLLAYVILTYILLLNMLIALMGETVNKIAQESKNIWKLQRAITILDTEKSFLKCMRK 719

FIILLLAYVILTYILLLNMLIALMGETVNKIAQESKNIWKLQRAITILDTEKSFLKCMRK Sbjct: 659 FIILLLAYVILTYILLLNMLIALMGETVNKIAQESKNIWKLQRAITILDTEKSFLKCMRK 718

Query: 720 AFRSGKLLQVGYTPDGKDDYRWCFRVDEVNWTTWNTNVGIINEDPGNCEGVKRTLSFSLR 779

AFRSGKLLQVG+TPDGKDDYRWCFRVDEVNWTTWNTNVGIINEDPGNCEGVKRTLSFSLR Sb_jct: 719 AFRSGKLLQVGFTPDGKDDYRWCFRVDEVNWTTWNTNVGIINEDPGNCEGVKRTLSFSLR 778

Query: 780 SSRVSGRHWKNFALVPLLREASARDRQSAQPEEVYLRQFSGSLKPEDAEVFKSPAASGEK 839

S RVSGR+WKNFALVPLLR+AS RDR + Q EEV L+ ++GSLKPEDAEVFK GEK Sbjct: 779 SGRVSGRNWKNFALVPLLRDASTRDRHATQQEEVQLKHYTGSLKPEDAEVFKDSMVPGEK 838

Pedant information for DKFZphtes3_20k2, frame 2 Report for DKFZphtes3_20k2 .2

[LENGTH] 839

[MW] 94950 . 75

[pi ] 6 . 90

[HOMOL] TREMBL:AF029310_1 product: "vamlloid receptor subtype 1"; Rattus norvegicus vamlloid receptor subtype 1 mRNA, complete eds. 0.0

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YIL112w] 4e-05

[PIRKW] alternative splicing 3e-06

[PIRKW] peripheral membrane protein 3e-06

[SUPFAM] ankyrin repeat homology 3e-06

[SUPFAM] unassigned ankyrin repeat proteins 3e-06

[PFAM] Ank repeat

[KW] TRANSMEMBRANE 4

SEQ MKKWSSTDLGAAADPLQKDTCPDPLDGDPNSRPPPAKPQLSTAKSRTRLFGKGDSEEAFP PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc MEM

SEQ VDCPHEEGELDΞCPTITVSPVITIQRPGDGPTGARLLΞQDSVAASTEKTLRLYDRRSIFE

PRD cccccccccccccccccceeeeeeecccccccceeeccccccccccchhhhhhhhhhhhh

MEM

SEQ AVAQNNCQDLESLLLFLQKSKKHLTDNEFKDPETGKTCLLKAMLNLHDGQNTTIPLLLEI

PRD hhhhcchhhhhhhhhhhhhhcccccccccccccccchhhhhhhhhhccccccchhhhhhh

MEM

SEQ ARQTDSLKELVNASYTDSYYKGQTALHIAIERRNMALVTLLVENGADVQAAAHGDFFKKT

PRD hhhcccccccccccccccccccchhhhhhhhhcchhhhhhhhhccceeeccccccccccc

MEM

SEQ KGRPGFYFGELPLSLAACTNQLGIVKFLLQNSWQTADISARDSVGNTVLHALVEVADNTA

PRD ccccceeeccccchhhhhhcchhhhhhhhhcccccccccccccccchhhhhhhhhhcccc

MEM

SEQ DNTKFVTSMYNEILILGAKLHPTLKLEELTNKKGMTPLALAAGTGKIGVLAYILQREIQE

PRD chhhhhhhhhhhhhhhccccccceeeeeecccccccchhhhhhhcchhhhhhhhhhhhhc

MEM

SEQ PECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSSETPNRHDMLLVEPLN

PRD ccccchhhhhheeeccceeeeeeeccccccccccccceeeeeccccccccceeeeehhhh

MEM

SEQ RLLQDKWDRFVKRIFYFNFLVYCLYMIIFTMAAYYRPVDGLPPFKMEKIGDYFRVTGEIL

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccccccchhhhhhhhc

MEM MMMMMMMMMMMMMMMMM

SEQ SVLGGVYFFFRGIQYFLQRRPSMKTLFVDSYSEMLFFLQSLFMLATVVLYFSHLKEYVAS

PRD cccceeeeeecchhhhhhhhheeeeeeccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

MEM MMMMMMMMMMMMMMMMM

SEQ MVFSLALGWTNMLYYTRGFQQMGIYAVMIEKMILRDLCRFMFVYIVFLFGFSTAVVTLIE

PRD hhhhhhhhhhhhheeecccccccchhhhhhhhhhhhhhhhhhhheeecccccceeeeeec

MEM MMMMMMMMMMMMMMMMM.

SEQ DGKNDSLPSESTSHRWRGPACRPPDSSYNSLYSTCLELFKFTIGMGDLEFTENYDFKAVF

PRD cccccccccccccccccccccccccccccchhhhhhhhhhhhhccccchhhhhhhhhhhh

MEM MM

SEQ IILLLAYVILTYILLLNMLIALMGETVNKIAQESKNIWKLQRAITILDTEKSFLKCMRKA

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

MEM MMMMMMMMMMMMMMM

SEQ FRSGKLLQVGYTPDGKDDYRWCFRVDEVNWTTWNTNVGIINEDPGNCEGVKRTLSFSLRS

PRD hhcceeeeeecccccccccceeeeeeecccccccccceeeecccccccceeeeeeeeeec

MEM

SEQ SRVSGRHWKNFALVPLLREASARDRQSAQPEEVYLRQFSGSLKPEDAEVFKSPAASGEK

PRD ccccccccccchhhhhhhhhhhhhhhcccccceeeeecccccccccceeeecccccccc

MEM

(No Prosite data available for DKFZphtes3_20k2.2)

Pfam for DKFZphtes3_20k2.2 HMM_NAME Ank repeat

HMM *GyTPLHIAARyNNvEMVrlLLQHGADIN*

G+T+LHIA +++N+ +V LL+++GAD+ Query 202 GQTALHIAIERRNMALVTLLVENGADVQ 229

DKFZphtes3_2013

group: transmembrane protein

DKFZphtes3_2013 encodes a novel 595 amino acid protein with partial similarity to the IL-17 receptor.

The novel protein contains one transmembrane region.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes and as a new marker for testicular cells. similarity to IL-17 receptor

Sequenced by MediGenomix

Locus : unknown

Insert length: 2406 bp

Poly A stretch at pos. 2345, no polyadenylation signal found

1 GCCTCAGGTG TTCCTGCGTT GTTTGTCAGT GGAGAGCAGG GAGTGGGGCC

51 AGCCAGCAGA AACAGTGGGC TGTACAACAT CACCTTCAAA TATGACAATT

101 GTACCACCTA CTTGAATCCA GTGGGGAAGC ATGTGATTGC TGACGCCCAG

151 AATATCACCA TCAGCCAGTA TGCTTGCCAT GACCAAGTGG CAGTCACCAT

201 TCTTTGGTCC CCAGGGGCCC TCGGCATCGA ATTCCTGAAA GGATTTCGGG

251 TAATACTGGA GGAGCTGAAG TCGGAGGGAA GACAGTGCCA ACAACTGATT

301 CTAAAGGATC CGAAGCAGCT CAACAGTAGC TTCAAAAGAA CTGGAATGGA

351 ATCTCAACCT TTCCTGAATA TGAAATTTGA AACGGATTAT TTCGTAAAGG

401 TTGTCCCTTT TCCTTCCATT AAAAACGAAA GCAATTACCA CCCTTTCTTC

451 TTTAGAACCC GAGCCTGTGA CCTGTTGTTA CAGCCGGACA ATCTAGCTTG

501 TAAACCCTTC TGGAAGCCTC GGAACCTGAA CATCAGCCAG CATGGCTCGG

551 ACATGCAGGT GTCCTTCGAC CACGCACCGC ACAACTTCGG CTTCCGTTTC

601 TTCTATCTTC ACTACAAGCT CAAGCACGAA GGACCTTTCA AGCGAAAGAC

651 CTGTAAGCAG GAGCAAACTA CAGAGATGAC CAGCTGCCTC CTTCAAAATG

701 TTTCTCCAGG GGATTATATA ATTGAGCTGG TGGATGACAC TAACACAACA

751 AGAAAAGTGA TGCATTATGC CTTAAAGCCA GTGCACTCCC CGTGGGCCGG

801 GCCCATCAGA GCCGTGGCCA TCACAGTGCC ACTGGTAGTC ATATCGGCAT

851 TCGCGACGCT CTTCACTGTG ATGTGCCGCA AGAAGCAACA AGAAAATATA

901 TATTCACATT TAGATGAAGA GAGCTCTGAG TCTTCCACAT ACACTGCAGC

951 ACTCCCAAGA GAGAGGCTCC GGCCGCGGCC GAAGGTCTTT CTCTGCTATT

1001 CCAGTAAAGA TGGCCAGAAT CACATGAATG TCGTCCAGTG TTTCGCCTAC

1051 TTCCTCCAGG ACTTCTGTGG CTGTGAGGTG GCTCTGGACC TGTGGGAAGA

1101 CTTCAGCCTC TGTAGAGAAG GGCAGAGAGA ATGGGTCATC CAGAAGATCC

1151 ACGAGTCCCA GTTCATCATT GTGGTTTGTT CCAAAGGTAT GAAGTACTTT

1201 GTGGACAAGA AGAACTACAA ACACAAAGGA GGTGGCCGAG GCTCGGGGAA

1251 AGGAGAGCTC TTCCTGGTGG CGGTGTCAGC CATTGCCGAA AAGCTCCGCC

1301 AGGCCAAGCA GAGTTCGTCC GCGGCGCTCA GCAAGTTTAT CGCCGTCTAC

1351 TTTGATTATT CCTGCGAGGG AGACGTCCCC GGTATCCTAG ACCTGAGTAC

1401 CAAGTACAGA CTCATGGACA ATCTTCCTCA GCTCTGTTCC CACCTGCACT

1451 CCCGAGACCA CGGCCTCCAG GAGCCGGGGC AGCACACGCG ACAGGGCAGC

1501 AGAAGGAACT ACTTCCGGAG CAAGTCAGGC CGGTCCCTAT ACGTCGCCAT

1551 TTGCAACATG CACCAGTTTA TTGACGAGGA GCCCGACTGG TTCGAAAAGC

1601 AGTTCGTTCC CTTCCATCCT CCTCCACTGC GCTACCGGGA GCCAGTCTTG

1651 GAGAAATTTG ATTCGGGCTT GGTTTTAAAT GATGTCATGT GCAAACCAGG

1701 GCCTGAGAGT GACTTCTGCC TAAAGGTAGA GGCGGCTGTT CTTGGGGCAA

1751 CCGGACCAGC CGACTCCCAG CACGAGAGTC AGCATGGGGG CCTGGACCAA

1801 GACGGGGAGG CCCGGCCTGC CCTTGACGGT AGCGCCGCCC TGCAACCCCT

1851 GCTGCACACG GTGAAAGCCG GCAGCCCCTC GGACATGCCG CGGGACTCAG

1901 GCATCTATGA CTCGTCTGTG CCCTCATCCG AGCTGTCTCT GCCACTGATG

1951 GAAGGACTCT CGACGGACCA GACAGAAACG TCTTCCCTGA CGGAGAGCGT

2001 GTCCTCCTCT TCAGGCCTGG GTGAGGAGGA ACCTCCTGCC CTTCCTTCCA

2051 AGCTCCTCTC TTCTGGGTCA TGCAAAGCAG ATCTTGGTTG CCGCAGCTAC

2101 ACTGATGAAC TCCACGCGGT CGCCCCTTTG TAACAAAACG AAAGAGTCTA

2151 AGCATTGCCA CTTTAGCTGC TGCCTCCCTC TGATTCCCCA GCTCATCTCC

2201 CTGGTTGCAT GGCCCACTTG GAGCTGAGGT CTCATACAAG GATATTTGGA

2251 GTGAAATGCT GGCCAGTACT TGTTCTCCCT TGCCCCAACC CTTTACCGGA

2301 TATCTTGACA AACTCTCCAA TTTTCTAAAA TGATATGGAG CTCTGAAAAA

2351 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

2401 AAAAAA

BLAST Results o BLAST result Medline entries

No Medline entry

Peptide information for frame 1

ORF from 346 bp to 2130 bp; peptide length: 595 Category: similarity to known protein Classification: unclassified

1 MESQPFLNMK FETDYFVKVV PFPSIKNESN YHPFFFRTRA CDLLLQPDNL

51 ACKPFWKPRN LNISQHGSDM QVSFDHAPHN FGFRFFYLHY KLKHEGPFKR

101 KTCKQEQTTE MTSCLLQNVS PGDYIIELVD DTNTTRKVMH YALKPVHSPW

151 AGPIRAVAIT VPLVVISAFA TLFTVMCRKK QQENIYSHLD EESSESSTYT

201 AALPRERLRP RPKVFLCYSS KDGQNHMNVV QCFAYFLQDF CGCEVALDLW

251 EDFSLCREGQ REWVIQKIHE SQFIIVVCSK GMKYFVDKKN YKHKGGGRGS

301 GKGELFLVAV SAIAEKLRQA KQSSSAALSK FIAVYFDYSC EGDVPGILDL

351 STKYRLMDNL PQLCSHLHSR DHGLQEPGQH TRQGSRRNYF RSKSGRSLYV

401 AICNMHQFID EEPDWFEKQF VPFHPPPLRY REPVLEKFDS GLVLNDVMCK

451 PGPESDFCLK VEAAVLGATG PADSQHESQH GGLDQDGEAR PALDGSAALQ

501 PLLHTVKAGS PSDMPRDSGI YDSSVPSSEL SLPLMEGLST DQTETSSLTE

551 SVSSSSGLGE EEPPALPΞKL LSSGSCKADL GCRSYTDELH AVAPL

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_2013, frame 1

TREMBL :U58917_1 product: "IL-17 receptor"; Homo sapiens IL-17 receptor mRNA, complete eds., N = 1, Score = 215, P = 4.7e-14

TREMBL :MM31993_1 product: "interleukin 17 receptor"; Mus musculus interleukin 17 receptor mRNA, complete eds., N = 2, Score = 152, P = 1. le-13

>TREMBL:U58917_1 product: "IL-17 receptor"; Homo sapiens IL-17 receptor mRNA, complete eds. Length = 866

HSPs:

Score = 215 (32.3 bits), Expect = 4.7e-14, P = 4.7e-14 Identities = 85/284 (29%), Positives = 131/284 (46%)

Query: 213 KVFLCYSSKDGQNHMNVVQCFAYFLQDFCGCEVALDLWEDFSLCREGQREWV-IQK I 268

KV++ YS+ D +++VV FA FL CG EVALDL E+ ++ G WV QK + Sbjct: 379 KVWIIYSA-DHPLYVDVVLKFAQFLLTACGTEVALDLLEEQAISEAGVMTWVGRQKQEMV 437

Query: 269 HESQFIIVVCSKGMKY FVDKKNYXXXXXXXXXXXXELFLVAVSAIAEXXXXXXXXX 324

+ IIV+CS+G + + + +LF A++ I

Sbjct: 438 ESNSKIIVLCSRGTRAKWQALLGRGAPVRLRCDHGKPVGDLFTAAMNMILPDFKRPACFG 497

Query: 325 XXXXXXFIAVYF-DYSCEGDVPGILDLSTKYRLMDNLPQLCSHLHSRDHGLQEPGQHTRQ 383

++ YF + SC+GDVP + + +Y LMD ++ + +D + +PG+ R Sbjct: 498 T YVVCYFSEVSCDGDVPDLFGAAPRYPLMDRFEEV--YFRIQDLEMFQPGRMHRV 550

Query: 384 G—SRRNYFRSKSGRSLYVAICNMHQFIDEEPDWFEKQFV PFHPPPLR YREPV 434

G S NY RS GR L A+ + PDWFE + + P L + EP+ Sbjct: 551 GELSGDNYLRSPGGRQLRAALDRFRDWQVRCPDWFECENLYSADDQDAPSLDEEVFEEPL 610

Query: 435 LEKFDSGLVLNDVMCKPGPESDFCLKVEAAVLGATGPADSQHESQHGGLDQDGEARP 491

L +G+V + + P S CL ++ V G G A H L G+ P Sb_jct: 611 LPP-GTGIVKRAPLVRE-PGSQACLAIDPLV-GEEGGAAVAKLEPH—LQPRGQPAP 662

Pedant information for DKFZphtes3_2013, frame 1

Report for DKFZphtes3_2013.1 [ LENGTH] 595

[MW] 66847 . 05

[pi ] 6 . 27

[ HOMOL] TREMBL : MM31993 1 product "interleukin 17 receptor " ; Mus musculus interleukin 17 receptor mRNA, complete eds . 2e- 14 [ BLOCKS ] BL00740A MAM domain proteins

[ BLOCKS ] BL01224 B N-acetyl-gamma-glutamyl-phosphate reductase proteins

[ KW] TRANSMEMBRANE 1

[ KW] LOW_COMPLEXITY 13 . 61 %

SEQ MESQPFLNMKFETDYFVKVVPFPSIKNESNYHPFFFRTRACDLLLQPDNLACKPFWKPRN

SEG

PRD ccccccccccccccceeeeeccccccccccceeeeeeceeeeeeeccccccccccccccc

MEM

SEQ LNISQHGSDMQVSFDHAPHNFGFRFFYLHYKLKHEGPFKRKTCKQEQTTEMTSCLLQNVS

SEG

PRD eeeecccccceeeecccccccceeeeeehhhhhhcccchhhhhhhhhhhhhhhhhhcccc

MEM

SEQ PGDYIIELVDDTNTTRKVMHYALKPVHSPWAGPIRAVAITVPLVVISAFATLFTVMCRKK

SEG

PRD ccceeeeeeccccccccccccccccccccccccceeeeccchhhhhhhhhhhhhhhhhhh

MEM MMMMMMMMMMMMMMMMM

SEQ QQENIYSHLDEESSESSTYTAALPRERLRPRPKVFLCYΞSKDGQNHMNVVQCFAYFLQDF

SEG xxxxxxx xxxxxxxxxx

PRD hhhhhhhhhcccccccceeeeccccccccccceeeeeeecccccchhhhhhhhhhhhhhc

MEM

SEQ CGCEVALDLWEDFSLCREGQREWVIQKIHESQFIIVVCSKGMKYFVDKKNYKHKGGGRGS

SEG xxxxxxxxx

PRD ccchhhhhhhhccccccccchhhhhhhhhhheeeeeeeeccceeeeeccccccccccccc

MEM

SEQ GKGELFLVAVSAIAEKLRQAKQSSSAALSKFIAVYFDYSCEGDVPGILDLSTKYRLMDNL

SEG XXX xxxxxxxxxxxxxx

PRD ccceeeeehhhhhhhhhhhhhhcchhhhhhhheeeeccccccccccccccchhhhhhccc

MEM

SEQ PQLCSHLHSRDHGLQEPGQHTRQGSRRNYFRSKSGRSLYVAICNMHQFIDEEPDWFEKQF

SEG

PRD cchhhhhhcccccccccccccccccceeeeccccccceeeeeeceeeecccccceeeeee

MEM

SEQ VPFHPPPLRYREPVLEKFDSGLVLNDVMCKPGPESDFCLKVEAAVLGATGPADSQHESQH

SEG

PRD eecccccccccceeeeeccccceeeeecccccccccchhhhhhhhhhccccccccccccc

MEM

SEQ GGLDQDGEARPALDGSAALQPLLHTVKAGSPSDMPRDΞGIYDSSVPSSELΞLPLMEGLST

SEG xxxxxxxxxxxxxxxxx .

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccchh

MEM

SEQ DQTETSSLTESVSSSSGLGEEEPPALPSKLLSSGSCKADLGCRSYTDELHAVAPL

SEG .. xxxxxxxxxxxxxxxxxxxx

PRD hhhhhhhhheeecccccccccccccccceeeccccceeeccccccccceeeeccc

MEM

(No Prosite data available for DKFZphtes3_2013.1) (No Pfam data available for DKFZphtes3_2013.1) DKFZphtes3_20ml8

group: nucleic acid management

DKFZphtes3_20ml8 encodes a novel 132 amino acid protein with similarity to the S. cerevisiae mitochondrial carrier protein RIM2.

The novel protein contains a leucine zipper and a Prosite mitochondrial energy transfer proteins signature. It is member of a family of substrate carrier proteins which are found in the inner mitochondrial membrane and are involved m energy transfer. The RIM2/MRS12 gene encodes a predicted protein of 377 ammo acids that is essential for mitochondrial DNA metabolism and proper cell growth. Inactivation of this gene causes the total loss of mitochondrial DNA and, compared to wild-type rhoo controls, a slow-growth phenotype on media containing glucose. The novel protein seems to be the human orthologue of this protein.

The new protein can find application in modulation of mitochondrial DNA replication and maintenance. similarity to carrier protein RIM2

Sequenced by MediGenomix

Locus : unknown

Insert length: 3572 bp

Poly A stretch at pos. 3530, polyadenylation signal at pos. 3510

1 GCCGCGGGGA GGGCTGTGCC GGTTGCTTTC TGCAGCCGCA TCTCGGCCAG

51 CTCTCCTCGC CGTCCCCGGG GCGCTGTGCG TCTCCAGTCC GGGACCGAAG

101 CCGCCTGCCG TAGCGGGCGG CCAGATCCGC GTCCCGCCTC AGCGGCCGGA

151 GGACATGCGG GAGAGAGAAT GAGCCAGAGG GACACGCTGG TGCATCTGTT

201 TGCCGGAGGA TGTGGTGGTA CAGTGGGAGC TATTCTGACA TGTCCACTGG

251 AAGTTGTAAA AACACGACTG CAGTCATCTT CTGTGACGCT TTATATTTCT

301 GAAGTTCAGC TGAACACCAT GGCTGGAGCC AGTGTCAACC GAGTAGTGTC

351 TCCCGGACCT CTTCATTGCC TAAAGGTGAT CTTGGAAAAA GAAGGGCCTC

401 GTTCCTTGTT TAGAGGACTA GGCCCCAATT TAGTGGGGGT AGCCCCTTCC

451 AGAGCAATAT ACTTTGCTGC TTATTCAAAC TGCAAGGAAA AGTTGAATGA

501 TGTATTTGAT CCTGATTCTA CCCAAGTACA TATGATTTCA GCTGCAATGG

551 CAGGTATGAA TGTATAATAT TAAAAAAAAA AAAAACTTTC TGAAACCTAG

601 AGGCTTAATA TTGAATTATA AGTTTGTAGT GAAAAGTTGA TGATTAATGT

651 GCTTTTCATT GATTAGATGA TTTTTACGTT TATCGATATA AACCAAATTA

701 GGTATATGTA AAATCTGTCA TCAGTTGACA TTTTTGTAGT CAGGAGTTTA

751 CATGCTAGGG TACAAGTAAT ATATTTATAT TGCCTTGTGT AGTCCACTGA

801 ATGTTTAGTG ATCATTGTTA ACAGTTTTAA GAATCCAACC ATAATTACAC

851 TATAAATAAG TTATGGAGCT GTAATTTACT CTTCTCTCCT CAATTTCTGT

901 TAGTGCCTTT TCCCTTTTTG CTGCATGTTT TGGCTTCTGT CTGAAATGTG

951 TCGGCAATTC TTGGTAAAGT ATTCATTTTG TCCTGTGCTC AAATGCTGAA

1001 ATTTTTGTGA GTGATGTATT ATTATTGACA ATTCAGTTAC TATGTGTATT

1051 TTTTAAAATT GTTTATTATT CTACATAATT CACACTAGAC AGCACCTGAA

1101 ATTTAGACAC TGGCTATGTG TACATGCTTA CTATAGAAAT GTTTCCAGGA

1151 ACTCTCTGTT TCTGTCATCA CTGATAAGTA TATATGATTC TGAATTAAAA

1201 TAACTAGTTT TAGGTCTTTA CCCTGCCATA AAGATAAACA GTTGGTTTGA

1251 CCAATCTGGT TCTGGAATCA TTTGCTGCTA TGCATGTTAG ACAAAGCCAC

1301 GAACTTTGAT TTTCCATTGA AAATTCTCCC TAATATCTGA GATTTATTGT

1351 ATATTTACTC ATATCTCACA TTTTCAAATT ATGCTGTAAC TTTATAAACT

1401 GTAGCTGCTT TCATCAGCTA TTGATCAATA AATTGAATGT CAATTATGTG

1451 CTTAATAATG AGTGCCTTAA ACTGTTAAAC ACTTTTGGTT TAGAAATAAA

1501 GTGAATCAAT TTGACCTATA TACTTCATGA AGTAAGTAAG TTTGAAATAC

1551 AAATTTCTGA AAGGTCAATA GCCCTTATCG TATTACAAAT TGTTTTTAAG

1601 GCTTTTTGTA TTTATTAATT GTCAGTTGAT TCACTGAAGC TTTAAAACTG

1651 GAAGGGACAA TCCAAAGGTC AAAAGAGTGA AATACAATCA TTTACCAATA

1701 AGGAAACCTT GGGCAAATTA TGTAATTTAT GTGAACCTCT CTTAGCTTAC

1751 CCATGGAATG AGTCAAGTGG TCTACATAGA TTTGGATTTT GAGAATTAGT

1801 TCTTTCATTT AGTGTTATAG AGATTATCTT GTTACAACTA GAATTATTTT

1851 TAATGTAATT TTTACAGATG TTGAATATTA GTAGATAGGA TTTTTCCCCT

1901 ACGAATTTGG ATGTAAGGTA AAGGTTGGTG GCCAGTGACA AACCTTATAA

1951 CCACTTTATC AGGTTCTTTA AAAATATATT TGTGAATTAC CAGTGATTAT

2001 GTTTTTGGCT TATAACCTCA GATAATTATA AAGAAATGTT AATCTTATTT

2051 GAAAGAATTG GAATCTAGAA AGTTAGATGA GCAGTCATTT TATATTGATA

2101 TTTGTTATAT CAGTATAGCA AATGCAGAGG TTCAGAATAT CTTTATTTCC

2151 ACTGGAACAT CTTATTTCAT TAGAGTATCT CATCAGAATT TATTACTGTA

2201 TTTGTATCAC ATTGCAAAGA ATTTCAGTAG AATTGTCAGT TTGCACTTTT

2251 TTCTCAAATG TGTACAAATG TTAACATATA GTTCATTTTT ATCTGTACAT

2301 TGATGCCATT TCCCAACTTG AATTCCTCAA GTTTTGGTAA ACTTACAATC

2351 TCATACTTGT TCAGAGGTTA TTGCACTGTA CACTTACTGT GTAGAAAATA

2401 CTGTTTGAAT TTGTTTGCAG TTACATTGTT CTGAGAACTG TGCTCTCAGA

2451 GCTTCTGTGC ACTATTCATG AGCATTAACA CTTAGCCTTG CAGTTTTATA 2501 CATAACTATA TGGTTAGTAA AACTGAATGG TCCAATGCAG ACTCATTAAA

2551 GTAGGCTTTT GCCCCCTTTG TTCTTGAAAT AATCTAGACC AGATTACTCG

2601 GGGTTTTTTT TAGGATTATT TTTATAGGTC TAAATATGAA TGATTTGGGG

2651 GTATGAAGTA CTTAAAGATA GTTCTGTGAA AAATCATTTT CAGCTGTCTA

2701 TTCAAGGGAA AAAATGCTAA CCTTGTCACT TTACTACACA AAACCACACT

2751 AAAATAAACC ATTAATGATA CTGCCTGCAA GATTTTAACA CACCAGATAG

2801 CACACACATT AAGGATTTAT AAGGCACTGT ACGTAATTTT TATTCCAAGT

2851 GACCTCTCAA TTCATTTTCA TTTTGCATTT TATCCATATG AACTCATGTT

2901 TAATTTAGAT AATAAAAATT TATTTTATTA AAAGGACAGT TTATTTAAAG

2951 TGGGTCTTTT TATTTGTTGT AGTGCATACT ATAAGAATTT GTAAGCCTCT

3001 AAAGTTGAGC TATAAATTTT CATGCATTAA AAATTTGTTT CAGTTGTGAG

3051 GATATTTAAT CAGATTAAAT AATGTTGACT CTTAATATTT TGCCTGCCTT

3101 TTTTTTCTCC TACACATGAC CTTTGACAGA CTAAGTATAT CTCAGCTATT

3151 GAGGGTATCT GTTTTGTTGC CTGTATATTT TGTTTAAATT AACTTGTATA

3201 TTCCTTTGTA TACACCTAGG CACAGATGTA TGCAAAAAAA ATTTGTTAAA

3251 TTACTTCTTT CTTTATACTA ATTCTCAATT TTTAAAAGAT TTTATCTGGC

3301 ATGTATATAC TTTTATATAG AACATTATAA ATGTAAAGGA AATGAATTCT

3351 AATTTTAATT GGATTATGTA TTCATACAGT TATTCTCAAT TTTTAAAATA

3401 CTAATAATGT AATCATTGAA TGTTTCCTAC ATACGTAGTG GGTTTTATTT

3451 GCTCACAGCA TACAGTTATT TTTCAATTTA TGTTTTTCTA TTAGACTTAA

3501 ATTTCATTAT AATAAAGGCT TTTACTCATT AAATACAAAA AAAAAAAAAA

3551 AAAAAAAAAA AAAAAAAAAA AA

BLAST Results No BLAST result

Medlme entries

No Medl e entry

95198680:

Overexpression of a novel member of the mitochondrial carrier family rescues defects m both

DNA and RNA metabolism m yeast mitochondria.

Peptide information for frame 1

ORF from 169 bp to 564 bp; peptide length: 132 Category: similarity to known protein Classification: Intacellular transport and traffic Prosite motifs: LEUCINE_ZIPPER (27-49) MITOCH CARRIER (26-36)

1 MSQRDTLVHL FAGGCGGTVG AILTCPLEVV KTRLQSSSVT LYISEVQLNT 51 MAGASVNRVV SPGPLHCLKV ILEKEGPRSL FRGLGPNLVG VAPSRAIYFA 101 AYSNCKEKLN DVFDPDSTQV HMISAAMAGM NV

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_20ml8, frame 1

PIR:Ξ44092 probable carrier protein c2 - Caenorhabditis elegans, N = 2, Score = 147, P = 1.5e-19

PIR:S36081 probable carrier protein RIM2, mitochondrial - yeast (Saccharomyces cerevisiae), N = 1, Score = 230, P = 6.2e-19

>PIR:S36081 probable carrier protein RIM2, mitochondrial - yeast (Saccharomyces cerevisiae) Length = 377

HSPs:

Score = 230 (34.5 bits), Expect = 6.2e-19, P = 6.2e-19 Identities = 55/133 (41%), Positives = 80/133 (60%)

Query: 8 VHLFAGGCGGTVGAILTCPLEVVKTRLQSSS-VTLYISEVQLNTMAGA SVNRVVSP 62

VH AGG GG GA++TCP ++VKTRLQS + Y S+ +N G+ S+N V+ Sbjct: 54 VHFVAGGIGGMAGAVVTCPFDLVKTRLQSDIFLKAYKSQA-VNISKGSTRPKSINYVIQA 112 Query: 63 GP LHCLKVILEKEGPRSLFRGLGPNLVGVAPSRAIYFAAYSNCKEKLNDVFD—P 115

G L + + ++EG RSLF+GLGPNLVGV P+R+I F Y K+ F+ Sbjct: 113 GTHFKETLGIIGNVYKQEGFRSLFKGLGPNLVGVIPARSINFFTYGTTKDMYAKAFNNGQ 172

Query: 116 DSTQVHMIΞAAMAG 129

++ +H+++AA AG Sbjct: 173 ETPMIHLMAAATAG 186

Score = 77 (11.6 bits), Expect = l.le+00, P = 6.8e-01

Identities = 25/88 (28%), Positives = 39/88 (44%)

Query: 3 QRDTLVHLFAGGCGGTVGAILTCPLEVVKTRLQSSΞVTLYISEVQLNTMAGASVNRVVSP 62 Q ++HL A G A T P+ ++KTR VQL+ SV + +

Sbjct: 172 QETPMIHLMAAATAGWATATATNPIWLIKTR VQLDKAGKTSVRQYKNS 2 IS

Query: 63 GPLHCLKVILEKEGPRSLFRGLGPNLVG 90

CLK ++ EG L++GL + +G

Sbjct: 220 WD--CLKSVIRNEGFTGLYKGLSASYLG 245

Score = 71 (10.7 bits), Expect = 6.6e+00, P = l.Oe+00

Identi .ties ^:= 28/91 (30%), Positives = 45/91 (49%)

Query: 12 AGGCGGTVGAILTCPLEVVKTRLQSSSVTLYISEVQLNTMAGASVNRVVSPGPLHCLKVI 71 + G V +1 T P EVV+TRL+ + + N G R + G + KVI

Sbjct: 294 SAGLAKFVASIATYPHEVVRTRLRQTP KEN G KRKYT-GLVQΞFKVI 338

Query: 72 LEKEGPRSLFRGLGPNLVGVAPSRAIYFAAY 102 +++EG S++ GL P+L+ P+ I F +

Sbjct: 339 IKEEGLFSMYSGLTPHLMRTVPNSIIMFGTW 369

Pedant information for DKFZphtes3_20ml8, frame 1

Report for DKFZphtes3_20ml8.1

[LENGTH) 132

[MW] 13993 .36

[pl] 8.42

[HOMOL] PIR:S 36081 probable carrier protein RIM2, mitochondrial - yeast (Saccharomyces cerevisiae) 7e-19

[ FUNCAT ] 07 . 16 purine and pyπmidme transporters [S. cerevisiae, YBR192w] 3e-20

[FUNCAT] 08 . 04 mitochondrial transport [S. cerevisiae, YBR192w] 3e-20

[ FUNCAT] 30 . 16 mitochondrial organization [S. cerevisiae, YBR192w] 3e-20

[FUNCAT] 02 . 13 respiration [S. cerevisiae, YBR192w] 3e-20

[FUNCAT] 01 . 05 .07 carbohydrate transport [S. cerevisiae, YPR021c] 3e-10

[FUNCAT] 07 . 07 sugar and carbohydrate transporters [S. cerevisiae, YPR021c] 3e-10

[FUNCAT] 07 . 99 other transport facilitators [S. cerevisiae, YEL006w] le-09

[FUNCAT] 01 . 07 .10 transport of vitamins, cofactors, and prosthetic groups [S. cerevisiae, YIL006w] 3e-09

[ FUNCAT] 07.04 .07 amon transporters (cl, so4, po4, etc.) [S. cerevisiae, YKL120w]

2e-08

[FUNCAT] 01.03.19 nucleotide transport [S. cerevisiae, YPROllc] 3e-08

[FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YKR052c] 4e-08

[FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YJR095w]

2e-07

[FUNCAT] 01.01.07 ammo-acid transport [S. cerevisiae, YOR130c] 5e-05

[FUNCAT] 07.10 amino-acid transporters [S. cerevisiae, YOR130c] 5e-05

[FUNCAT] 01.04.07 phosphate transport [S. cerevisiae, YJR077c] 7e-05

[FUNCAT] 13.04 homeostasis of other ions [S. cerevisiae, YJR077c] 7e-05

[BLOCKS] BL00215B Mitochondrial energy transfer proteins

[BLOCKS] BL00215A Mitochondrial energy transfer proteins

[PIRKW] duplication 6e-09

[PIRKW] transmembrane protein 6e-09

[PIRKW] mitochondrial inner membrane 4e-07

[PIRKW] transport protein 5e-06

[PIRKW] mitochondrion 7e-08

[PIRKW] chloroplast 3e-08

[SUPFAM] Btl protein 3e-08

[SUPFAM] ADP, ATP carrier protein repeat homology 4e-09

[SUPFAM] Caenorhabditis probable carrier protein c2 4e-09

[SUPFAM] probable carrier protein YPR021c 6e-09

[PROSITE] LEUCINE_ZIPPER 1

[PROSITE] MITOCH_CARRIER 1

[PFAM] Mitochondrial carrier proteins

[KW] Alpha_Beta

SEQ MSQRDTLVHLFAGGCGGTVGAILTCPLEVVKTRLQSSSVTLYISEVQLNTMAGASVNRVV PRD cccccceeeecccccccceeeeeecchhhhhhhhhhhccccccccccccccccccccccc

SEQ SPGPLHCLKVILEKEGPRSLFRGLGPNLVGVAPSRAIYFAAYSNCKEKLNDVFDPDSTQV PRD cccchhhhhhhhhhcccceeeeccccceeeecccceeeeeehhhhhhhhhcccccccccc

SEQ HMISAAMAGMNV PRD chhhhhhhcccc

Prosite for DKFZphtes3_20ml8.1

PS00029 27->49 LEUCINE_ZIPPER PDOC00029 PS00215 26->36 MITOCH CARRIER PDOC00189

Pfam for DKFZphtes3_20ml8.1

HMM_NAME Mitochondrial carrier proteins

HMM *pFwkdFLAGGIAGmMeHTvMFPIDtIKTRMQlQgEMpM.. ahpR

+++++++AGG +G + +++++P++++KTR+Q++ ++ + ++

Query 5 DTLVHLFAGGCGGTVGAILTCPLEVVKTRLQSS-SVTLYISEVQLNTMA 52

HMM YkGMIdCFRwIwkNEGWRGLWRGLgANvIRYIPqWalRFGFY

G+++C++ I+++EG+R+L+RGLG+N+++++P +AI+F+ Y

Query 53 GASVNRVVSPGPLHCLKVILEKEGPRSLFRGLGPNLVGVAPSRAIYFAAY 102

HMM EFMKeMFiDyfgeddnyWmWFwmnYMaGs* +KE ++D F++ D++++++ + +MAG+

Query 103 SNCKEKLNDVFDP-DSTQVHMISAAMAGM 130

DKFZphtes3_21d4

group: signal transduction

DKFZphtes3_21d4 encodes a novel 464 ammo acid putative GTP exchanging factor related to RCCl.

RCCl (regulator of chromosome condensation) is a eukaryotic protein which binds to chromatin and interacts with ran, a nuclear GTP-binding protein. RCCl promotes the exchange of bound GDP with GTP, acting as a guamne-nucleotide dissociation stimulator.

The new protein can find application in the regulation of gene expression by activition of nuclear GTP-binding proteins. The X-linked retinitis pigmentosa is a result of a defect GTPase regulator, which contains a RCCl-type repeat. similarity to RCCl-like G exchanging factor RLG complete cDNA, complete eds, EST hits

Sequenced by LMU

Locus: /map="20"

Insert length: 2321 bp

Poly A stretch at pos. 2293, polyadenylation signal at pos. 2262

1 GGGTCACGCA AGATGGCGGC GCCCAGAGGC TGCTGAGGCG CGGAACGGAG

51 GATGGCGCTG GTGGCGTTGG TGGCTGGGGC TCGGCTGGGG CGGCGGCTGA

101 GCGGGCCGGG GCTGGGGCGA GGGCACTGGA CGGCGGCCAG GCGCTCCCGG

151 AGCCGGCGCG AAGCGGCAGA AGCCGAGGCG GAGGTGCCCG TGGTCCAGTA

201 CGTGGGCGAG CGCGCTGCCC GCGCCGATCG CGTCTTCGTG TGGGGCTTCA

251 GCTTCTCGGG GGCGCTGGGC GTGCCTTCCT TTGTGGTGCC CAGCTCCGGG

301 CCCGGGCCCC GCGCCGGCGC CCGACCGCGC CGCAGGATCC AGCCCGTGCC

351 CTATCGCCTG GAGCTGGACC AAAAGATTTC ATCTGCTGCT TGCGGCTATG

401 GATTCACACT GCTGTCCTCT AAGACTGCGG ATGTTACGAA AGTCTGGGGG

451 ATGGGACTCA ACAAAGATTC TCAGCTTGGA TTTCACAGGA GCCGGAAAGA

501 TAAAACGAGG GGCTACGAGT ATGTGTTGGA GCCCTCACCC GTCTCCCTGC

551 CTCTGGACAG ACCTCAGGAG ACACGGGTGC TGCAGGTCTC CTGCGGCCGA

601 GCTCACTCTC TTGTGTTGAC TGACAGGGAA GGAGTCTTCA GCATGGGAAA

651 CAATTCTTAT GGGCAATGTG GAAGAAAGGT GGTCGAAAAT GAAATTTACA

701 GTGAAAGTCA CAGAGTCCAC AGGATGCAGG ACTTCGATGG CCAGGTGGTC

751 CAGGTCGCCT GTGGTCAGGA TCATAGTCTG TTCCTGACGG ATAAAGGAGA

801 AGTCTATTCT TGTGGATGGG GTGCTGATGG GCAAACAGGT CTGGGTCACT

851 ACAATATCAC CAGCTCGCCC ACCAAGCTGG GTGGAGACCT GGCGGGAGTG

901 AACGTTATCC AAGTTGCCAC CTACGGTGAT TGCTGCCTGG CCGTGTCCGC

951 CGACGGAGGA CTTTTTGGTT GGGGAAACTC GGAGTACCTG CAGCTGGCCT

1001 CTGTCACTGA CTCCACACAG GTGAATGTGC CCCGCTGCTT ACACTTCTCA

1051 GGAGTGGGGA AGGTGCGACA GGCTGCATGC GGTGGCACGG GCTGTGCAGT

1101 GTTAAACGGA GAAGGACATG TTTTTGTCTG GGGCTATGGA ATTCTTGGGA

1151 AAGGTCCAAA CCTAGTGGAA AGTGCCGTCC CTGAAATGAT TCCACCCACT

1201 CTCTTTGGCT TGACGGAGTT CAACCCAGAA ATCCAGGTTT CCCGCATCCG

1251 ATGTGGACTC AGCCACTTTG CTGCACTGAC CAACAAAGGA GAGCTGTTTG

1301 TATGGGGCAA GAACATCCGA GGGTGCCTGG GAATCGGTCG CCTGGAGGAC

1351 CAGTATTTCC CATGGAGGGT GACGATGCCT GGGGAGCCTG TGGACGTGGC

1401 ATGTGGCGTG GACCACATGG TGACCCTGGC CAAGTCATTC ATCTAAACCT

1451 CCCTCACCTG CTTGGGCGGC CCCGTCCCGG GAACCACTGG CACTCCTTGG

1501 CAGAGGCCAG CGCGTGGCCA GCCCCCCGGG GTTCTTGGAT GGTGGTGGCG

1551 GAGGACCCTG CGTGCAGTGT GACGCTCTGT CCTGAATCCC TTAGCGGGTA

1601 CCTACCAGGA GGATCAGGGC AAGGTCCCTC TCCAGCTGCA GGTGAGGCCT

1651 GCGGAACTCA GCTTGGATGG CAGCCTTTGG TGGGCCGCTG TGGCCCGCAC

1701 GTCTCTGTTC TCTCCAAGTA ACATGCGACG GTGTCTGGTG TCACGTCTCG

1751 CCTGAGAAGC CCGTCTTAGG AAAGCTTAGC TTGAACACAG TGCTCGGGAG

1801 GTTTCTGCTC TGTCTGTCAT GGCAGTCTCT TGGTTTGTGT CTGGCCAAGG

1851 CCATGCGTGT GCCTCGGACC GAGCCCCAGC TTAGGCGAGG GAGTCAGGCT

1901 GGCTTCGGCC CTCGGTTTTC ATTCAGGCCA CCCTGCTCAT GGCCCTTCCT

1951 GGCCGCCTGC CACACCGCAA GCTCGCTGGG GGGACACTAG AAGCACCGTG

2001 GCCTGGGATT CCATCTGGAG CTGTCCGCAG GCACCAGCCC CAGCCTCCCA

2051 CCACGCTCAC TGCCTGGCTT GGAAAAGTTA AGAAGCCCCT CAGGAAGAGA

2101 ATCGAGGCTA AGTTCCTCTG CGCCGAGGGC CCCGAGCATA TCCGCCAAGG

2151 CTCAGCTGCA GTGCCAGGCG GAGGAGGAAG ATCCAGAAAT TGTGAACAAT

2201 GTTTGATTTA GTAGCGTGAC TTGCCTTTCC CTTTAAAAAC ATCTTTTACA

2251 AATCTGTCTT GGAATAAAGT CTATTTTCTG CCTTTTGGTT TTTAAAAAAA

2301 AAAAAAAAAA AAAAAAAAAA A

BLAST Results Entry HS203358 from database EMBL: human STS SHGC-31781. Score = 1748, P = l.le-72, identities = 376/394

Medline entries

No Medline entry

Peptide information for frame 1

ORF from 52 bp to 1443 bp; peptide length: 464 Category: similarity to known protein

1 MALVALVAGA RLGRRLSGPG LGRGHWTAAR RSRSRREAAE AEAEVPVVQY 51 VGERAARADR VFVWGFSFSG ALGVPSF VP SSGPGPRAGA RPRRRIQPVP 101 YRLELDQKIS SAACGYGFTL LSSKTADVTK VWGMGLNKDS QLGFHRSRKD 151 KTRGYEYVLE PSPVSLPLDR PQETRVLQVS CGRAHSLVLT DREGVFSMGN 201 NSYGQCGRKV VENEIYSEΞH RVHRMQDFDG QVVQVACGQD HΞLFLTDKGE 251 VYΞCGWGADG QTGLGHYNIT SSPTKLGGDL AGVNVIQVAT YGDCCLAVSA 301 DGGLFGWGNS EYLQLASVTD STQVNVPRCL HFSGVGKVRQ AACGGTGCAV 351 LNGEGHVFVW GYGILGKGPN LVESAVPEMI PPTLFGLTEF NPEIQVSRIR 401 CGLSHFAALT NKGELFVWGK NIRGCLGIGR LEDQYFPWRV TMPGEPVDVA 451 CGVDHMVTLA KSFI

BLASTP hits

Entry CEW09G3_5 from database TREMBLNEW: gene: "W09G3.3"; Caenorhabditis elegans cosmid W09G3

Score = 395, P = 9.3e-37, identities = 111/330, positives = 165/330

Entry Y032_HUMAN from database SWISSPROT:

HYPOTHETICAL PROTEIN KIAA0032.

Score = 309, P = 1.0e-24, identities = 96/308, positives = 143/308

Entry B38919 from database PIR: hypothetical protein 2 - human (fragment)

Score = 309, P = 1.0e-24, identities = 96/308, positives = 143/308

Entry AF060219_1 from database TREMBLNEW: product: "RCCl-like G exchanging factor RLG"; Homo sapiens RCCl-like G exchanging factor RLG mRNA, complete eds.

Score = 273, P = 4.0e-21, identities = 84/262, positives = 124/262

Entry S71752 from database PIR: giant protein p619 - human

Score = 282, P = 1. le-19, identities = 86/287, positives = 144/287

Alert BLASTP hits for DKFZphtes3_21d4, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphtes3_21d4, frame 1

Report for DKFZphtes3_21d4.1

[LENGTH] 464

[MW] 49997.08

[pi] 8.74

[HOMOL] TREMBL :CEW09G3_5 gene: "W09G3.3"; Caenorhabditis elegans cosmid W09G3 5e-34

[FUNCAT] 04.07 rna transport [S. cerevisiae, YGL097w] 2e-09

[FUNCAT] 03.07 pheromone response, matmg-type determination, sex-specific proteins

[S. cerevisiae, YGL097w] 2e-09

[FUNCAT] 08.01 nuclear transport [S. cerevisiae, YGL097w] 2e-09

[FUNCAT] 04.05.05 mrna processing (5'-end, 3'-end processing and mrna degradation) [S. cerevisiae, YGL097w] 2e-09

[FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YGL097w] 2e-09

[FUNCAT] 04.03.03 trna processing [S. cerevisiae, YGL097w] 2e-09

[FUNCAT] 30.03 organization of cytoplasm [Ξ. cerevisiae, YGL097w] 2e-09 [FUNCAT] 30.04 organization of cytoskeleton [Ξ. cerevisiae, YAL020c] 4e-06

[BLOCKS] BL00870I

[BLOCKS] BL00625B Regulator of chromosome condensation (RCCl) proteins

[BLOCKS] BL00625A Regulator of chromosome condensation (RCCl) proteins

[PIRKW] blocked ammo end 3e-16

[PIRKW] nucleus 3e-16

[PIRKW] duplication 4e-08

[PIRKW] tandem repeat 3e-16

[PIRKW] DNA binding 3e-16

[PIRKW] mitosis 3e-16

[PIRKW] leucine zipper 3e-21

[SUPFAM] pheromone response pathway component SRM1 4e-08

[SUPFAM] WD repeat homology 3e-21

[PROSITE] MYRISTYL 7

[PROSITE] RCC1_2 2

[PROSITE] AMIDATION 2

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 5

[PROSITE] TYR_PHOSPHO_SITE 2

[PROSITE] GLYCOSAMINOGLYCAN 3

[PROSITE] PKC_PHOSPHO_SITE 7

[PROSITE] ASN_GLYCOSYLATION 2

[PFAM] Regulator of chromosome condensation (RCCl)

[KW] All_Beta

[KW] LOW COMPLEXITY 13.58 %

SEQ MALVALVAGARLGRRLSGPGLGRGHWTAARRSRSRREAAEAEAEVPWQYVGERAARADR SEG . xxxxxxxxxxxxxxxxxxxxxxx ...xxxxxxxxxxxxxxxxx PRD ccchhhhhhhhhheeeccccccccchhhhhhhhhhhhhhhhhhhceeeeeehhhhhhhhh

SEQ VFVWGFSFSGALGVPΞFVVPSSGPGPRAGARPRRRIQPVPYRLELDQKISSAACGYGFTL SEG xxxxxxxxxxxxxxxxxxxxxxx PRD eeeeccccccccccceeeeeccccccccccccccccccccchhhhhhhheeeccccceee

SEQ LΞSKTADVTKVWGMGLNKDSQLGFHRSRKDKTRGYEYVLEPSPVSLPLDRPQETRVLQVS SEG PRD eecccccceeeeccccccccccccccccccccccceeeeeccccccccccccccceeeee

SEQ CGRAHSLVLTDREGVFSMGNNSYGQCGRKVVENEIYSESHRVHRMQDFDGQVVQVACGQD SEG PRD cccceeeeeeccceeeeeccccccccccccccccccccccccccccccceeeeeeecccc

SEQ HSLFLTDKGEVYSCGWGADGQTGLGHYNITSSPTKLGGDLAGVNVIQVATYGDCCLAVΞA SEG PRD eeeeeecccceeeecccccccccccccccccccccccccccceeeeeeecccceeeeeec

SEQ DGGLFGWGNSEYLQLASVTDSTQVNVPRCLHFSGVGKVRQAACGGTGCAVLNGEGHVFVW SEG PRD ccceeeeccccccccccccccccccccccccccccceeeeeccccceeeeeecccceeee

SEQ GYGILGKGPNLVESAVPEMIPPTLFGLTEFNPEIQVSRIRCGLSHFAALTNKGELFVWGK SEG PRD cccccccccccccccccccccceeeeeeecccceeeeeeecccceeeeeecccceeeecc

SEQ NIRGCLGIGRLEDQYFPWRVTMPGEPVDVACGVDHMVTLAKSFI SEG PRD cccccccccccccccccceeecccceeeeecccccccccccccc

Prosite for DKFZphtes3_21d4.1

PΞ00001 200->204 ASN_GLYCOSYLATION PDOC00001 PS00001 268->272 ASN_GLYCOSYLATION PDOC00001 PS00002 17->21 GLYCOSAMINOGLYCAN PDOC00002 PS00002 82->86 GLYCOSAMINOGLYCAN PDOC00002 PS00002 333->337 GLYCOSAMINOGLYCAN PDOC00002 PS00004 14->18 CAMP_PHOSPHO_SITE PDOC00004 PS00005 34->37 PKC_PHOSPHO_SITE PDOC00005 PS00005 122->125 PKC_PHOSPHO_SITE PDOC00005 PS00005 147->150 PKC_PHOSPHO_SITE PDOC00005 PS00005 190->193 PKC_PHOSPHO_SITE PDOC00005 PS00005 219->222 PKC_PHOSPHO_SITE PDOC00005 PS00005 246->249 PKC_PHOSPHO_SITE PDOC00005 PS00005 410->413 PKC_PHOSPHO_SITE PDOC00005 PS00006 34->38 CK2_PHOSPHO_SITE PDOC00006 PS00006 147->151 CK2_PHOSPHO_SITE PDOC00006 PS00006 190->194 CK2_PHOSPHO_SITE PDOC00006 PS00006 290->294 CK2_PHOSPHO_SITE PDOC00006 PS00006 317->321 CK2 PHOSPHO SITE PDOC00006 PS00007 209->217 TYR_PHOSPHO_SITE PDOC00007 PS00007 208->217 TYR_PHOSPHO_SITE PDOC00007 PS00008 9->15 MYRISTYL PDOC00008 PS00008 20->26 MYRISTYL PDOC00008 PS00008 133->139 MYRISTYL PDOC00008 PS00008 238->244 MYRISTYL PDOC00008 PS00008 277->283 MYRISTYL PDOC00008 PS00008 302->308 MYRISTYL PDOC00008 PS00008 344->350 MYRISTYL PDOC00008 PS00009 12->16 AMIDATION PDOC00009 PS00009 206->210 AMIDATION PDOC00009 PΞ00626 179->190 RCC1_2 PDOC00544 PS00626 235->246 RCCl 2 PDOC00544

Pfam for DKFZphtes3_21d4.1

HMM_NAME Regulator of chromosome condensation (RCCl)

HMM *IAaGqHHTVCLTqDGRVYtWG* +A GQ+H++ LT++G VY++G

Query 235 VACGQDHSLFLTDKGEVYSCG 255

DKFZphtes3_21jl5

group: transcription factors

DKFZphtes3_21jl5 encodes a novel 898 ammo acid protein with similarity human NY-CO-33 protein.

NY-CO-33 is a protein recognised by autologous antibodies of human colon cancer patients. The novel protein contains 4 C2H2 Zinc fingers and is a new putativ transcription factor.

The new protein can find application in modulating/blocking the expression of genes controlled by this transcription factor. strong similarity to "NY-CO-33" complete cDNA, complete eds, potential start at bp 27, EST hits

Sequenced by LMU

Locus : unknown

Insert length: 4407 bp

Poly A stretch at pos. 4321, polyadenylation signal at pos. 4301

1 CGCTGCAGCA GGTGTCACAG AGCCGCATGC TCCCGGAGCC CAGCCTCTTC

51 AGCACCGTGC AGCTGTACCG GCAGAGCAGC AAGCTCTATG GCTCCATCTT

101 CACGGGGGCC AGCAAGTTCC GCTGTAAGGA CTGCAGCGCT GCCTACGACA

151 CCCTGGTGGA GTTGACAGTG CACATGAACG AGACGGGGCA TTACCGCGAC

201 GACAACCATG AGACCGATAA CAACAACCCC AAGCGCTGGT CCAAGCCTCG

251 CAAACGCTCC TTGCTGGAAA TGGAAGGGAA GGAAGACGCC CAGAAGGTGC

301 TGAAGTGCAT GTACTGTGGC CACTCCTTTG AGTCCCTGCA GGATTTGAGT

351 GTCCATATGA TCAAAACAAA ACACTACCAA AAAGTGCCTC TGAAGGAACC

401 CGTCACTCCT GTCGCCGCCA AAATCATCCC TGCCACTCGG AAGAAAGCTT

451 CCCTGGAGCT GGAGCTCCCC AGCTCCCCAG ATTCCACAGG TGGAACCCCC

501 AAAGCCACCA TCTCAGACAC CAACGATGCA CTTCAGAAGA ACTCCAACCC

551 TTACATCACG CCAAATAATC GGTACGGCCA CCAGAATGGG GCCAGCTATG

601 CATGGCACTT TGAGGCCCGG AAGTCGCAGA TCCTGAAGTG CATGGAGTGT

651 GGGAGCTCGC ATGACACCCT GCAGGAGCTC ACTGCCCACA TGATGGTCAC

701 TGGCCACTTC ATCAAGGTCA CCAACTCTGC TATGAAAAAG GGGAAGCCCA

751 TTGTGGAGAC GCCTGTCACA CCTACCATCA CAACCCTGCT GGATGAGAAG

801 GTCCAGTCCG TGCCCCTGGC AGCCACCACC TTCACGTCCC CCTCCAATAC

851 ACCTGCCAGC ATCTCCCCAA AACTGAATGT GGAGGTCAAG AAGGAAGTCG

901 ACAAGGAGAA AGCGGTCACT GACGAGAAAC CTAAGCAAAA AGACAAGCCT

951 GGCGAAGAAG AGGAGAAGTG TGACATCTCT TCCAAATACC ATTACTTGAC

1001 TGAAAATGAC TTAGAAGAGA GTCCCAAGGG GGGGCTTGAT ATCCTCAAAT

1051 CCTTGGAAAA CACAGTGACA TCCGCAATCA ACAAGGCCCA GAACGGCACT

1101 CCTAGCTGGG GGGGCTATCC CAGCATCCAT GCCGCCTACC AACTTCCCAA

1151 CATGATGAAG TTGTCCCTGG GCTCGTCGGG GAAGAGCACG CCCCTGAAAC

1201 CCATGTTTGG CAACAGTGAG ATTGTCTCCC CGACGAAAAA CCAGACCCTG

1251 GTCTCTCCAC CCAGCAGCCA GACGTCCCCC ATGCCCAAGA CAAACTTTCA

1301 TGCCATGGAG GAGCTGGTGA AAAAGGTCAC TGAGAAAGTT GCCAAAGTGG

1351 AGGAGAAGAT GAAGGAGCCG GATGGGAAGC TTTCCCCGCC CAAGCGGGCC

1401 ACTCCCTCCC CATGTAGCAG CGAAGTCGGG GAACCCATCA AGATGGAGGC

1451 ATCCAGCGAT GGGGGCTTCC GCAGCCAGGA GAACAGCCCC AGCCCCCCGC

1501 GGGATGGGTG CAAGGATGGG AGCCCCCTCG CTGAGCCGGT GGAGAATGGC

1551 AAGGAGCTGG TGAAGCCCCT AGCCAGCAGT TTGAGTGGCA GCACGGCCAT

1601 CATCACCGAC CACCCGCCTG AACAGCCTTT TGTTAACCCT TTGAGCGCCC

1651 TGCAGTCAGT CATGAACATT CACCTGGGCA AGGCCGCCAA GCCCTCCCTG

1701 CCTGCCCTGG ACCCCATGAG CATGCTTTTC AAGATGAGCA ACAGCCTGGC

1751 GGAGAAGGCT GCTGTGGCCA CCCCGCCGCC CCTGCAGTCC AAGAAGGCAG

1801 ACCACCTCGA CCGCTATTTC TACCACGTCA ACAACGACCA GCCCATAGAC

1851 TTGACAAAAG GGAAGAGTGA CAAAGGCTGC TCCTTGGGTT CAGTGCTTCT

1901 GTCACCCACG TCCACAGCCC CGGCAACCTC CTCATCCACG GTGACAACGG

1951 CAAAGACATC TGCCGTCGTA TCATTCATGT CAAACTCGCC GCTACGCGAG

2001 AATGCCTTGT CAGATATATC CGATATGCTG AAGAACTTGA CAGAGAGCCA

2051 CACGTCAAAA TCCTCCACTC CTTCCAGCAT CTCCGAGAAG TCTGACATTG

2101 ACGGGGCCAC TCTGGAGGAG GCTGAGGAGT CGACGCCCGC CCAGAAGAGG

2151 AAGGGCCGCC AGTCAAACTG GAACCCCCAG CACCTCCTGA TCCTCCAGGC

2201 CCAGTTTGCC GCCAGCCTCC GGCAGACCTC AGAAGGGAAG TACATCATGT

2251 CAGACCTGAG CCCCCAGGAG CGGATGCATA TCTCCAGGTT CACCGGGCTG

2301 TCCATGACCA CCATCAGCCA CTGGCTGGCC AACGTGAAAT ACCAGCTTCG

2351 AAGGACAGGT GGAACAAAGT TCCTCAAAAA CTTGGACACT GGCCACCCCG

2401 TCTTCTTTTG TAACGATTGT GCGTCCCAAA TCAGGACTCC TTCCACGTAC

2451 ATCAGTCACC TAGAGTCACA CTTAGGCTTC CGGCTACGGG ACTTATCCAA

2501 ACTGTCCACC GAACAGATTA ACAGTCAGAT AGCACAAACC AAGTCACCGT

2551 CAGAAAAAAT GGTGACGTCC TCCCCCGAGG AAGACCTGGG GACTTCCTAT

2601 CAGTGCAAAC TTTGCAATCG GACCTTTGCC AGCAAGCACG CTGTTAAACT 2651 TCACCTTAGC AAAACACACG GGAAATCTCC GGAAGACCAC CTTCTGTATG

2701 TCTCTGAGTT AGAGAAGCAG TAGCATTTGC TTTTGATAGA AAGGACTGCA

2751 GTTTGCTTTG AGGGAAACTG TGGAAGGCAC CTTCAGGCCC CCTCTGACTT

2801 GTTGTTCTTG GCACATGTTC TTATTTTAAC TGCAGAGAAT CACTCTGGGC

2851 TGGACTGTTT TGTATAACTG TACAGTGTTT AATAGAGGTG CATAATCAGC

2901 TGTTGTTACT GGTAAAATAT GAAGGTTAAA ATGCAGTGGT AAGTGTTTGG

2951 AACTTTGTGT AAACGGGATT TAGTTGTGAG CATCCTCCCG ATGCTTCAAG

3001 CTGCATGCAT TAACAGACAG TTTAATTAAG CATTTATAAC GGAATCAGGC

3051 ACACCTTTTC CACGAGACTC GAGTGTGCTG GCATTTCTCA CCCTTTCATC

3101 TTTAGCCCTC TGAGTACTTT GAAGCACTTT TGCATTAATT TGGTTAAAAA

3151 ATAAAATAAA ATAATAATAA TGTATGAAGC TCTGTTTTTT AAACTCCTTA

3201 CCAGCTTAGT TATAATGAAT AATATGAACC TCCATTTATG CAGGTCTGCA

3251 GGGGTATAAC ACGCCTTGAA ATTTAAAAGA ATATTATTTT CACATTGAAA

3301 CATAGATGTA TATATTGTAT AGATTTCAGA CTCTCTTATG AAAAAAAATG

3351 TGATTGTGGT TAAATGACCT TTTTCTTGCA TTTATAGCAA CAGTGTTTTA

3401 TGCACCTGCT ATGCTCTGGG CATAAGCTGT GCCTATGTAT AGTGTATATT

3451 TCTTTTTTTC TTTTTTTTAA GGTCTATGGG TTTTGTTTTT TACATGCAAA

3501 CATTGTAAAT TATACAGAAG ATACCACAGA TAGCATTTAT AAAGTATACA

3551 GAAACATTAT CTGAAAGCAA AGTATGATAG TTTGTTTTGC TATACAGTAC

3601 ATCTATATTG ATAGAGGTTC ATGTTTAAAT TATACATATT TATTAGCATC

3651 ATATTGTCAT TTGTTTTGAG CAGTCTGAAT AAACGAGACC GGGAAAGACA

3701 TCCCTGGCAG GCATCAGAAC TATTTTGCAC ATGATTTTTA AAGGTATTTA

3751 TTAGAAATCA AAGAACACTC AAAATAAACT CAGTGCTCAA AGGGTTAAGT

3801 CTATTTGAAA AGGTTAAAAA AAAGAACAAA AAAAAAAAAA GAACTTGTAC

3851 TGTATTTCCT AAACATTGAT AAAGCCTTTA AAATGTTTGT ACTGTAATAC

3901 TTTGCTTAAA AGTCATGAGG CATTCTGTGA TCCAACCTCT TTCACTTATT

3951 TATAAGCCCT CTTGGTTGCT ATTCCATATT GTAGGATGCC TTTCTATTTC

4001 AATTGGTAAC TTTCTGTTTT GTTCTTCCTA ATTATTCTCC CAAGATCCCA

4051 CACTGCAGCT TTATCTTTAG GCTTATGAAA GGTAACCCGT GGTTACCGGC

4101 TCTCCAAGTG ATTCTGTTCT TCTCCATTTT TGGCAGTTAA TTTGCAGAAG

4151 TAACTGACAG CTGACACCAT ATGAGAACCT TTGTATAAAA TATTGGCATG

4201 TAAACAGCAC AGACACCGTA ACACACTCTG TGCCCTGTTT GGTTGTTGAC

4251 AATGAAGCAC CATTATGTGA CTCTTCATAT AACCCTTTTT TCTACGGCAG

4301 CATTAAAATT GTCTTTTTGC TATAAAAAAA AAAAAAAAAA AAAAAAAAAA

4351 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

4401 AAAAAAA

BLAST Results

No BLAST result

Medline entries

No Medlme entry

Peptide information for frame 3

ORF from 27 bp to 2720 bp; peptide length: 898 Category: strong similarity to known protein

1 MLPEPSLFST VQLYRQSSKL YGSIFTGASK FRCKDCSAAY DTLVELTVHM 51 NETGHYRDDN HETDNNNPKR WSKPRKRSLL EMEGKEDAQK VLKCMYCGHS 101 FESLQDLSVH MIKTKHYQKV PLKEPVTPVA AKIIPATRKK ASLELELPSS 151 PDSTGGTPKA TISDTNDALQ KNSNPYITPN NRYGHQNGAS YAWHFEARKS 201 QILKCMECGS SHDTLQELTA HMMVTGHFIK VTNSAMKKGK PIVETPVTPT 251 ITTLLDEKVQ SVPLAATTFT SPSNTPASIS PKLNVEVKKE VDKEKAVTDE 301 KPKQKDKPGE EEEKCDISSK YHYLTENDLE ESPKGGLDIL KSLENTVTSA 351 INKAQNGTPS WGGYPSIHAA YQLPNMMKLS LGSSGKSTPL KPMFGNSEIV 401 SPTKNQTLVS PPSSQTSPMP KTNFHAMEEL VKKVTEKVAK VEEKMKEPDG 451 KLSPPKRATP SPCSSEVGEP IKMEASSDGG FRSQENSPSP PRDGCKDGSP 501 LAEPVENGKE LVKPLASSLS GSTAIITDHP PEQPFVNPLS ALQSVMNIHL 551 GKAAKPSLPA LDPMSMLFKM SNΞLAEKAAV ATPPPLQSKK ADHLDRYFYH 601 VNNDQPIDLT KGKSDKGCSL GSVLLSPTST APATSSSTVT TAKTSAVVSF 651 MSNSPLRENA LSDISDMLKN LTESHTSKSS TPΞΞISEKSD IDGATLEEAE 701 ESTPAQKRKG RQSNWNPQHL LILQAQFAAS LRQTΞEGK I MSDLSPQERM 751 HISRFTGLSM TTISHWLANV KYQLRRTGGT KFLKNLDTGH PVFFCNDCAS 801 QIRTPSTYIS HLESHLGFRL RDLSKLSTEQ INSQIAQTKS PSEKMVTSSP 851 EEDLGTSYQC KLCNRTFASK HAVKLHLSKT HGKSPEDHLL YVSELEKQ

BLASTP hits

No BLASTP hits available Alert BLASTP hits for DKFZphtes3_21j 15, frame 3

TREMBL:AF039698_1 gene: "NY-CO-33"; product: "antigen NY-CO-33"; Homo sapiens antigen NY-CO-33 (NY-CO-33) mRNA, complete eds., N = 1, Score = 1039, P = 5.5e-105

PIR:A38437 probable homeotic protein tsh - fruit fly (Drosophila melanogaster), N = 3, Score = 158, P = 7.2e-09

TREMBL:CE33058_1 gene: "unc-89"; product: "UNC-89"; Caenorhabditis elegans UNC-89 (unc-89) gene, complete eds., N = 2, Score = 175, P = 3.3e-07

>TREMBL:AF039698_1 gene: "NY-CO-33"; product: "antigen NY-CO-33"; Homo sapiens antigen NY-CO-33 (NY-CO-33) mRNA, complete eds. Length = 687

HSPs:

Score = 1039 (155.9 bits), Expect = 5.5e-105, P = 5.5e-105 Identities = 244/504 (48%), Positives = 319/504 (63%)

Query: 170 QKNSNPYITPNNRYGHQNGASYAWHFEARKΞQILKCMECGSSHDTLQELTAHMMVTGHFI 229

QK +NPY+TPNNRYG+QNGASY W FEARK+QILKCMECGSSHDTLQ+LTAHMMVTGHF+ Sbjct: 14 QKAANPYVTPNNRYGYQNGASYTWQFEARKAQILKCMECGSSHDTLQQLTAHMMVTGHFL 73

Query: 230 KVTNSAMKKGKPIVETPVTPTITTLLDEKVQSVPLAATTFTS-PSNT PASISPKLN 284

KVT SA KKGK +V PV ++EK+QS+PL TT T P+++ P S + Sbjct: 74 KVTTSASKKGKQLVLDPV VEEKIQSIPLPPTTHTRLPASSIKKQPDSPAGΞTT 126

Query: 285 VEVKKEVDKEKA-VTDEKPKQKDKPGEEEEKCDISSKYHYLTENDLEESPKGGLDILKSL 343

E KKE +KEK V + K K++ + EK + S+ Y YL E DL++SPKGGLDILKΞL Sbjct: 127 SEEKKEPEKEKPPVAGDAEKIKEEΞEDSLEKFEPSTLYPYLREEDLDDSPKGGLDILKSL 186

Query: 344 ENTVTSAINKAQNGTPSWGGYPSIHAAYQLPNMMKLSLGSSGKSTPLKPMF-GNSEIVSP 402

ENTV++AI+KAQNG PΞWGGYPSIHAAYQLP +K L ++ +S ++P + G + +Ξ Sbjct: 187 ENTVSTAISKAQNGAPSWGGYPSIHAAYQLPGTVK-PLPAAVQSVQVQPSYAGGVKSLΞS 245

Query: 403 TKNQTLVSPPSSQTSPMPKTNFHAMEELVKKVTEKV-AKVEEKMKEPDGKLSPPKRATPS 461

++ L+ P S T P K+N AMEELV+KVT KV K EE+ E + K S K A S Sbjct: 246 AEHNALLHSPGSLTPPPHKSNVSAMEELVEKVTGKVNIKKEERPPEKE-KSSLAKAA—S 302

Query: 462 PCSSEVGEPIKMEASSDGGFRSQENSPSPPRDGCKDGSPLAEPVENGKELVKPLASSLSG 521

P + E + K E S + Q+ P K PL NG E +K ++ Sbjct: 303 PIAKENKDFPKTEEVSG KPQKKGPEAETWEAKKEGPLDVHTPNGTEPLKAKVTNGCN 359

Query: 522 STAIITDHPPEQPFVNPLSALQSVMNIHLGKAAKPSLPALDPMSMLFKMSNSLAEKAAVA 581

+ II DH PE F+NPLSALQS+MN HLGK +KP P+LDP++ML+K+SNS+ +K Sbjct: 360 NLGIIMDHSPEPSFINPLSALQSIMNTHLGKVSKPVSPSLDPLAMLYKISNSMLDKPVYP 419

Query: 582 TPPPLQSKKADHLDRYFYHVNNDQPIDLTKGKSDK-GCSLGSVLLSPTSTAPATSSSTVT 640

P K+AD +DRY+Y N+DQPIDLTK K+ S+ + SP + S + Sbjct: 420 ATPV KQADAIDRYYYE-NSDQPIDLTKSKNKPLVSSVADSVASPLRESALMDISDMV 475

Query: 641 TAKTSAVVΞFMSN-SPLRENALSDISDMLKNLTE 673

T + S S + E + +D Ξ + L E Sbjct: 476 KNLTGRLTPKSSTPSTVSEKSDADGΞSFEEALDE 509

Score = 865 (129.8 bits), Expect = 7.4e-95, P = 7.4e-95 Identities = 211/434 (48%), Positives = 268/434 (61%)

Query: 447 EPDGKLSPPKRATPSPCSSEVG—EPIKMEASSDGGFRSQENSPSPPRDG-CKDGSPLAE 503

E + L P TP P S V E + + + + +E P + K SP+A+ Sbjct: 247 EHNALLHSPGSLTPPPHKSNVSAMEELVEKVTGKVNIKKEERPPEKEKSSLAKAASPIAK 306

Query: 504 P-VE—NGKELVK-PLASSLSGSTAIITD-HPPE—QPFVNPLSALQSVMNIHLG 551

P E +GK K P A + D H P +P ++ + + I +

Sbjct: 307 ENKDFPKTEEVSGKPQKKGPEAETWEAKKEGPLDVHTPNGTEPLKAKVTNGCNNLGIIMD 366

Query: 552 KAAKPSLPALDPMSMLFKMSNSLAEKAAVATPPPLQSKKADHLDRYFYHVNN DQPID 608

+ +PS ++P+S L + N+ K + P L D L Y ++N D+P+ Sbjct: 367 HSPEPSF—INPLSALQSIMNTHLGKVSKPVSPSL DPL-AMLYKISNSMLDKPV- 417

Query: 609 LTKGKSDKGCSLGSVLLSPTSTAPATSSSTVTTAKTSAVVSFMSNSPLRENALSDISDML 668

K S P + + S+V ++ SPLRE+AL DISDM+

Sbjct: 418 -YPATPVKQADAIDRYYYENSDQPIDLTKSKNKPLVSSVADSVA-SPLRESALMDISDMV 475

Query: 669 KNLTESHTΞKSSTPSSISEKΞDIDGATLEEA-EESTPAQKRKGRQSNWNPQHLLILQAQF 727 KNLT T KSSTPS++SEKSD DG++ EEA +E +P KRKGRQSNWNPQHLLILQAQF Sbjct: 476 KNLTGRLTPKSSTPSTVSEKSDADGSSFEEALDELSPVHKRKGRQSNWNPQHLLILQAQF 535

Query: 728 AASLRQTSEGKYIMSDLSPQERMHISRFTGLSMTTISHWLANVKYQLRRTGGTKFLKNLD 787

A+SLR+T+EGKYIMSDL PQER+HIS+FTGLSMTTISHWLANVKYQLRRTGGTKFLKNLD Sbjct: 536 ASSLRETTEGKYIMSDLGPQERVHISKFTGLSMTTISHWLANVKYQLRRTGGTKFLKNLD 595

Query: 788 TGHPVFFCNDCASQIRTPSTYISHLESHLGFRLRDLΞKLSTEQINSQIAQTKSPSEKMV- 846

TGHPVFFCNDCASQ RT STYISHLE+HLGF L+DLSKL QI Q +K + K + Sbjct: 596 TGHPVFFCNDCASQFRTASTYISHLETHLGFSLKDLSKLPLNQIQEQQNVSKVLTNKTLG 655

Query: 847 -TSSPEEDLGTSYQCKLCNRTFASK 870

+ EEDLG+++QCKLCNRTFA + Sbjct: 656 PLGATEEDLGSTFQCKLCNRTFAKQ 680

Score = 98 (14.7 bits), Expect = 7.4e-95, P = 7.4e-95 Identities = 32/95 (33%), Positives = 47/95 (49%)

Query: 90 KVLKCMYCGHSFESLQDLSVHMIKTKHYQKVPL KEPVT-PVAAKIIPATRKKAS 142

++LKCM CG S ++LQ L+ HM+ T H+ KV K+ V PV + I + + Sbjct: 45 QILKCMECGSSHDTLQQLTAHMMVTGHFLKVTTSASKKGKQLVLDPVVEEKIQSIPLPPT 104

Query: 143 LELELPSS PDSTGGTPKATISDTNDALQKNSNP 175

LP+S PDS G+ T S+ +K P Sbjct: 105 THTRLPASSIKKQPDSPAGS TTSEEKKEPEKEKPP 139

Score = 81 (12.2 bits), Expect = 4.6e-93, P = 4.6e-93 Identities = 13/29 (44%), Positives = 20/29 (68%)

Query: 28 ASKFRCKDCSAAYDTLVELTVHMNETGHY 56

A +C +C +++DTL +LT HM TGH+ Sbjct: 44 AQILKCMECGSSHDTLQQLTAHMMVTGHF 72

Pedant information for DKFZphtes3_21j 15, frame 3

Report for DKFZphtes3_21j 15.3

[LENGTH] 898

[MW] 98486.72

[pi] 8.61

[HOMOL] TREMBL:AF039698_1 gene: "NY-CO-33"; product: "antigen NY-CO-33"; Homo sapiens antigen NY-CO-33 (NY-CO-33) mRNA, complete eds. 0.0

[BLOCKS] BL00028 Zmc finger, C2H2 type, domain proteins

[PIRKW] zmc fmger le-06

[PIRKW] DNA binding le-06

[PIRKW] transcription regulation le-06

[PROSITE] MYRISTYL 9

[PROSITE] ZINC_FINGER_C2H2 4

[PROSITE] CAMP_PHOSPHO_SITE 5

[PROSITE] CK2_PHOSPHO_SITE 19

[PROSITE] TYR_PHOSPHO_SITE 2

[PROSITE] PKC_PHOSPHO_SITE 15

[PROSITE] ASN_GLYCOSYLATION 4

[PFAM] Zinc finger, C2H2 type

[KW] Alpha_Beta

[KW] LOW_COMPLEXITY 11.36 %

SEQ MLPEPSLFSTVQLYRQSSKLYGSIFTGASKFRCKDCSAAYDTLVELTVHMNETGHYRDDN SEG

PRD ccccceeeeeeeeccccceeeeeeeccccceeecccchhhhhhhhhhhcccccccccccc

SEQ HETDNNNPKRWSKPRKRSLLEMEGKEDAQKVLKCMYCGHSFESLQDLSVHMIKTKHYQKV SEG

PRD cccccccccccccccchhhhhhhccchhhhhhhhhcccccchhhhheeeeeeeecceeee

SEQ PLKEPVTPVAAKIIPATRKKASLELELPSSPDSTGGTPKATISDTNDALQKNSNPYITPN SEG xxxxxxxxxx

PRD eccccccceeeeeeehhhhhhhhhhcccccccccccccceeeeccchhhhhccccccccc

SEQ NRYGHQNGASYAWHFEARKSQILKCMECGSSHDTLQELTAHMMVTGHFIKVTNSAMKKGK SEG

PRD ccccccccchhhhhhhhhhhhhhhhccccccccchhhhhhhhhhhceeeeeccccccccc

SEQ PIVETPVTPTITTLLDEKVQSVPLAATTFTSPSNTPASISPKLNVEVKKEVDKEKAVTDE

SEG xxxxxxxxxxxxx xxxxxxxxxxxxxxxx

PRD ccccccccccchhhhhhhhccccccccccccccccccccccccccccccccchhhhhhcc

SEQ KPKQKDKPGEEEEKCDISSKYHYLTENDLEESPKGGLDILKSLENTVTSAINKAQNGTPS SEG x PRD ccccccccccccccchhhhhhhhhhhcccccccccchhhhhhhhhhhhhhhhhhcccccc

SEQ WGGYPSIHAAYQLPNMMKLSLGSSGKSTPLKPMFGNSEIVSPTKNQTLVSPPSSQTSPMP SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ KTNFHAMEELVKKVTEKVAKVEEKMKEPDGKLSPPKRATPSPCSSEVGEPIKMEASSDGG SEG xxxxxxxxxxxxxxxxxxxx PRD ccchhhhhhhhhhhhhhhhhhhhhhcccccccccccccccccccccccccceeeeecccc

SEQ FRSQENSPSPPRDGCKDGSPLAEPVENGKELVKPLASSLSGSTAIITDHPPEQPFVNPLS SEG PRD cccccccccccccccccccccccccccccccccccccccccceeeeeccccccccccccc

SEQ ALQSVMNIHLGKAAKPSLPALDPMSMLFKMSNSLAEKAAVATPPPLQSKKADHLDRYFYH SEG PRD chhhhhhcccccccccccccchhhhhhhhhhhhhhccccccccccccccccccccceeee

SEQ VNNDQPIDLTKGKSDKGCSLGSVLLSPTSTAPATSSSTVTTAKTSAVVSFMSNSPLRENA SEG xxxxxxxxxxxxxxxxxxxxxxx PRD ecccccceeecccccccccccceeecccccccccccceeeeceeeeeeeeccccccchhh

SEQ LSDISDMLKNLTESHTSKSSTPSSISEKSDIDGATLEEAEESTPAQKRKGRQSNWNPQHL SEG xxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhcccccccccccceeecccccchhhhhhhhccchhhhhhcccccccchh

SEQ LILQAQFAASLRQTSEGKYIMSDLSPQERMHISRFTGLSMTTISHWLANVKYQLRRTGGT SEG PRD hhhhhhhhhhhhhccccceeecccccchhhhhhhhccccchhhhhhhhhhhhhhhhcccc

SEQ KFLKNLDTGHPVFFCNDCASQIRTPSTYISHLESHLGFRLRDLSKLSTEQINSQIAQTKS SEG PRD ceeecccccccceeecccceeeecccchhhhhhhhhhhhhhhhhcchhhhhhhhhhhhcc

SEQ PSEKMVTSSPEEDLGTSYQCKLCNRTFASKHAVKLHLSKTHGKSPEDHLLYVSELEKQ SEG PRD ccceeeeccccccccceeehhhhhhhhhhhhhhhhhccccccccccceeeeeeecccc

Prosite for DKFZphtes3_21jl5.3

PS00001 51->55 ASN_GLYCOSYLATION PDOC00001 PS00001 405->409 ASN_GLYCOSYLATION PDOC00001 PS00001 670->674 ASN_GLYCOSYLATION PDOC00001 PS00001 864->868 ASN_GLYCOSYLATION PDOC00001 PS00004 69->73 CAMP_PHOSPHO_SITE PDOC00004 PS00004 75->79 CAMP_PHOSPHO_SITE PDOC00004 PS00004 139->143 CAMP_PHOSPHO_SITE PDOC00004 PS00004 432->436 CAMP_PHOSPHO_SITE PDOC00004 PS00004 456->460 CAMP_PHOSPHO_SITE PDOC00004 PS00005 17->20 PKC_PHOSPHO_SI E PDOC00005 PS00005 137->140 PKC_PHOSPHO_SITE PDOC00005 PS00005 157->160 PKC_PHOSPHO_SITE PDOC00005 PS00005 280->283 PKC_PHOSPHO_SITE PDOC00005 PS00005 318->321 PKC_PHOSPHO_SITE PDOC00005 PS00005 332->335 PKC_PHOSPHO_SITE PDOC00005 PS00005 384->387 PKC_PHOSPHO_SITE PDOC00005 PS00005 435->438 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 588->591 PKC_PHOSPHO_SITE PDOC00005 PS00005 614->617 PKC_PHOSPHO_SITE PDOC00005 PS00005 641->644 PKC_PHOSPHO_SITE PDOC00005 PS00005 676->679 PKC_PHOSPHO_SITE PDOC00005 PS00005 686->689 PKC_PHOSPHO_SITE PDOC00005 PS00005 730->733 PKC_PHOSPHO_SITE PDOC00005 PS00005 842->845 PKC_PHOSPHO_SITE PDOC00005 PS00006 42->46 CK2_PHOSPHO_SITE PDOC00006 PS00006 78->82 CK2_PHOSPHO_SITE PDOC00006 PS00006 103->107 CK2_PHOSPHO_SITE PDOC00006 PS00006 149->153 CK2_PHOSPHO_SITE PDOC00006 PS00006 161->165 CK2_PHOSPHO_SITE PDOC00006 PS00006 210->214 CK2_PHOSPHO_SITE PDOC00006 PS00006 214->218 CK2_PHOSPHO_SITE PDOC00006 PS00006 253->257 CK2_PHOSPHO_SITE PDOC00006 PS00006 325->329 CK2_PHOSPHO_SITE PDOC00006 PS00006 573->577 CK2_PHOSPHO_SITE PDOC00006 PS00006 684->688 CK2_PHOSPHO_SITE PDOC00006 PS00006 689->693 CK2_PHOSPHO_SITE PDOC00006 PS00006 695->699 CK2_PHOSPHO_SITE PDOC00006 PS00006 745->749 CK2 PHOSPHO SITE PDOC00006 PS00006 810->814 CK2 PHOSPHO SITE PDOC00006

PS00006 840->844 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 848->852 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 884->888 CK2 PHOSPHO^" SITE PDOC00006

PS00006 893->897 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00007 732->740 TYR PHOSPHO^{" "}SITE PDOC00007

PS00007 883->892 TYR PHOSPHO^{" "}SITE PDOC00007

PS00008 22->28 MYRISTYL PDOC00008

PS00008 156->162 MYRISTYL PDOC00008

PS00008 188->194 MYRISTYL PDOC00008

PS00008 362->368 MYRISTYL PDOC00008

PS00008 479->485 MYRISTYL PDOC00008

PS00008 494->500 MYRISTYL PDOC00008

PS00008 498->504 MYRISTYL PDOC00008

PS00008 617->623 MYRISTYL PDOC00008

PS00008 757->763 MYRISTYL PDOC00008

PS00028 795->816 ZINC FINGER C2H2 PDOC00028

PS00028 860->882 ZINC FINGER^{" "}C2H2 PDOC00028

PS00028 33->56 ZINC FINGER^{" "}C2H2 PDOC00028

PS00028 94->117 ZINC FINGER^{" "}C2H2 PDOC00028

Pfam for DKFZphtes3_21jl5.3

HMM_NAME Zinc finger, C2H2 type

HMM *CpwPDCgKtFrrwsNLrRHMR..T.H* C++ C ++ + +L+ HM+ H

Query 33 CKD—CSAAYDTLVELTVHMNET-GH 55

26.69 (bits) f: 94 t: 116 Target: dkfzphtes3_21jl5.3 strong similarity to "NY-CO-33"

Alignment to HMM consensus : Query *CpwPDCgKtFrrwsNLrRHMR..T.H*

C + CG +F + +L HM+ H dkfzphtes3 94 CMY—CGHSFESLQDLSVHMIKT-KH 116

Query f: 795 t: 815 Target: dkfzphtes3_21j 15.3 strong similarity to "NY-CO-33"

Alignment to HMM consensus: HMM *CpwPDCgKtFrrwsNLrRHMRTH*

C++ C R++S+++ H+ +H Query 795 CND—CASQIRTPSTYISHLESH 815

27.12 (bits) f: 860 t: 881 Target: dkfzphtes3_21j 15.3 strong similarity to "NY-CO-33"

Alignment to HMM consensus : Query *CpwPDCgKtFrrwsNLrRHMR.T .H*

C+ C++TF +++ + H+ H dkfzphtes3 860 CKL—CNRTFASKHAVKLHLSK-TH 881

DKFZphtes3_21116

group: intracellular transport and trafficking

DKFZphtes3_21116 encodes a novel 66 amino acid protein nearly identical to rat ribosome attached membrane protein 4 (ramp4) .

The novel protein seems to be the human orthologe of rat ramp 4. Ramp4 is involved in the regulation of translocation of proteins into endoplasmic reticulum, e.g. of the MHC class II associated invariant (gamma) chain.

The new protein can find application in modulation of protein translocation into the endoplasmic reticulum.

identical to rat ribosome attached membrane protein 4

ORF Bp 316-513 (66 aa) see BLASTX

Sequenced by LMU

Locus : unknown

Insert length: 2488 bp

Poly A stretch at pos. 2464, polyadenylation signal at pos. 2442

1 CTTCCTCTTT CACTCCGCGC TCACGGCGGC GGCCAAAGCG GCGGCGACGG

51 CGGCGCGAGA ACGACCCGGC GGCCAGTTCT CTTCCTCCTG CGCACCTGCC

101 CCGCTCGGTC AGTCAGTCGG CGGCCGGCGC CCGGCTTGTG CTCAGACCTC

151 GCGCTTGCGG CGCCCAGGCC CAGCGGCCGT AGCTAGCGTC TGGCCTGAGA

201 ACCTCGGCGC TCCGGCGGCG CGGGCACCAC GAGCCGAGCC TCGCAGCGGC

251 TCCAGAGGAG GCAGGCGAGT GAGCGAGTCC GAGGGGTGGC CGGGGCAGGT

301 GGTGGCGCCG CGAAGATGGT CGCCAAGCAA AGGATCCGTA TGGCCAACGA

351 GAAGCACAGC AAGAACATCA CCCAGCGCGG CAACGTCGCC AAGACCTCGA

401 GAAATGCCCC CGAAGAGAAG GCGTCTGTAG GACCCTGGTT ATTGGCTCTC

451 TTCATTTTTG TTGTCTGTGG TTCTGCAATT TTCCAGATTA TTCAAAGTAT

501 CAGGATGGGC ATGTGAAGTG ACTGACCTTA AGATGTTTCC ATTCTCCTGT

551 GAATTTTAAC TTGAACTCAT TCCTGATGTT TGATACCCTG GTTGAAAACA

601 ATTCAGTAAA GCATCCTGCC TCAGAATGAC TTTCCTATCA TGCTTCATGT

651 GTCATTCCAA GGTTTCTTCA TGAGTCATTC CAAGTTTTCT AGTCCATACC

701 ACAGTGCCTT GCAAAAAACA CCACATGAAT AAAGCAATAA AATTTGATTG

751 TTAAGATACA GTAGTGGACC CTACTTATTC AGTCAATTAA GAGTAAGTTT

801 TTTTATGTGG TTATTAAAAC AGTATGAACA ATTAGTCTAA CTCTGCATAG

851 ACAGGGTCTA GATTTTGTTA ACCCAAATGT ATAACTGCAG TTAGCTTAAA

901 TTACAATTTG AAGTCTTGTG GTTTTTATAT AGCTAGGCAC TTTATTACTC

951 TTTTGAACTG AAAGCACACT CCCTTATAGG TTCATGTAAC TGTCCTGTAA

1001 TAAGGTGCTT ATAAATGGAA CAACTACACA GCCTAGTTTT GCCACAACCT

1051 TTAGCATCTA AAAAGTTTTA AAAGCTTCTA AATGTCTAAT ATAAAGGGAG

1101 ATGCTTATAG CCACAACATC TATTTTACCA ATATTGTTTC CATTACACTA

1151 CCTTGGATTT TGCATGAGTG AGTATAGTAA CCCAAGATGC CATAAAAAAA

1201 AACTTGATCG TTTTCTGACT TAATTAGTTA CTGTGGTTTC ACTAAAAGCT

1251 ACCGTGGTGG AGTGAAGTCA GTCAGGGAAG GTTTGTTTAT GTTACATTTA

1301 TTTCACCAGA ACTATTTTAA TATATCAAAG GGGTTTACTA TGCCAAACAA

1351 AATTCTAGGG AAAAATACTG CTAAAAATGG ATGCCTCATC AGAACATGCT

1401 GTTGAGTCCA ATGTGCCATA AGACATTTTA GCATGTTAAA TAGCACTTTT

1451 AATAGCAAAA AAAGGCACAT CAACTGCGAA GTTATCCTTA GTTTGCAAAT

1501 GCTTTTTCTA GATTAATGAT TTTTCAATCA TTAGGGTACT AGACACATCA

1551 GCCTAAAGTG GCATCTGGAA TTGAATGGAT TTACTGATAA TGATCAGTCT

1601 TTAGTCTTCC CTTTGTTATA TGACTTTATA GGTTATGATT GATCAAATTT

1651 ACGTTTTACT AATGGTAAGG GTGAGGGTCA TAGGGCAGGT TTTGGGTTTT

1701 CTAGTACTGT TGAAAACTGC AAGTATTGGC TATTTGTATA CTTAGCCATA

1751 ACTTGGTGAA AAAAAACCTG AGCAGTGTCT ATGTATTAAT GCGTTGGAAA

1801 GAAAGCTGCT TGTGTTTGCT TTGTTAATTG CCTCAGGATA TTTCTTTTAA

1851 AATAAGCTGT TTTAAGAGGA ACAGAAGGGA AATCTGCTAC CTAGTCTATA

1901 CACAGCGTGA ACCTCACAGG GGGCTTCTGA TACCCTCAAA CATGGAGAAC

1951 AGTAAGGGAG CAGAGTGGTT AAGGACTTTC AGGAACTTAA CTATTCTGGA

2001 ATAAGGAATG AATCAACTGA CCTTGGGCCA GCAGGTTTTT AACTAAATTG

2051 TTACTTGCCT TTCTCACCCA GTTAATCAGT CTCTGTACTT GTTTCCCTTT

2101 TTGAAACAAG TGTCTTGGTT AACTAATTCT GTTTTATGGT TGTGCTAAAT

2151 TCATAGCAGG TGCCTTATTC TTTGCTTTTA GTCAAACCAT TCCATATCAG

2201 AATTTTCCTT GGTTTACTAT AGATATTTGG CTTTAAGTTG TTGTTTGTGT

2251 TTTTTAATGT ACAATGTTCT GATAAATTTG ACTGTTAAAT TGCTATAGCT

2301 AGCAATCATT TTACATATGT AAAAAATTGC ATTCCCTTTG TATTTCATGT

2351 GTAATTCACC AATTAAGTGC AGTTTATATT CAGGTTGGAT TATGCATGTT

2401 TAGGTAAACG AAAGCTGTGT CTTACTTGAT TTATTCTTTA AAAATAAAGT

2451 TCCCTGAATA TTTGAAAAAA AAAAAAAAAA AAAAAAAA BLAST Results

Entry HSCDN13 from database EMBL:

H. sapiens (TL5) mRNA from LNCaP cell line

Score = 1075, P = 5.8e-41, identities = 219/221

Entry AF100470_1 from database TREMBLNEW: gene: "RAMP4"; product: "ribosome attached membrane protein 4"; Rattus norvegicus ribosome attached membrane protein 4 (RAMP4) mRNA, complete eds .

Score = 331, P = 3.9e-28, identities = 66/66, positives = 66/66, frame

+1

Entry HSG19910 from database EMBL: human STS A002B48. Score = 530, P = 2.1e-17, identities = 108/109

Medline entries

No Medline entry

Peptide information for frame 1

ORF from 316 bp to 513 bp; peptide length: 66 Category: strong similarity to known protein Classification: Intacellular transport and traffic

1 MVAKQRIRMA NEKHSKNITQ RGNVAKTSRN APEEKASVGP WLLALFIFW 51 CGSAIFQIIQ SIRMGM

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_21116, frame 1

TREMBLNEW:RN0238236_1 gene: "ramp4"; product: "ribosome associated membrane protein RAMP4"; Rattus norvegicus mRNA for ribosome associated membrane protein RAMP4, N = 1, Score = 331, P = 6.2e-30

TREMBL:AF100470_1 gene: "RAMP4"; product: "ribosome attached membrane protein 4"; Rattus norvegicus ribosome attached membrane protein 4 (RAMP4) mRNA, complete eds., N = 1, Score = 331, P = 6.2e-30

>TREMBLNEW:RN0238236_1 gene: "ramp4"; product: "ribosome associated membrane protein RAMP4"; Rattus norvegicus mRNA for ribosome associated membrane protein RAMP4

Length = 75

HSPs:

Score = 331 (49.7 bits), Expect = 6.2e-30, P = 6.2e-30 Identities = 66/66 (100%), Positives = 66/66 (100%)

Query: 1 MVAKQRIRMANEKHSKNITQRGNVAKTΞRNAPEEKASVGPWLLALFIFVVCGSAIFQIIQ 60

MVAKQRIRMANEKHSKNITQRGNVAKTSRNAPEEKASVGPWLLALFIFVVCGSAIFQIIQ Sbjct: 10 MVAKQRIRMANEKHSKNITQRGNVAKTSRNAPEEKASVGPWLLALFIFVVCGSAIFQIIQ 69

Query: 61 SIRMGM 66

SIRMGM Sbjct: 70 SIRMGM 75

No Pedant data available DKFZphtes3_21n23 group: testes derived

DKFZphtes3_15j 18 encodes a novel 148 amino acid protein with strong similarity to rat 7acomp protein.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . strong similarity to rat 7acomp protein on genomic level encoded by AF107885

Sequenced by LMU

Locus : /map="14q24.3"

Insert length: 3122 bp

Poly A stretch at pos. 3070, polyadenylation signal at pos. 3045

1 GGAAAACCTC GTGGGCTCAG CCCGGGAGAA AGGGCCAGGG AAGTTGGGTG 51 GTTCTGTGCT TGGTCTGTCA ATGGAGGAGA TCAAAGTTTT ACGAAGGGTG

101 AAGGAGGAGA ATGATCGGCG AGGTGGATTT ATTCGCATAT TTCCTACATC

151 TGAGACATGG GAAATATATG GGTCCTACCT CGAGCATAAG ACCTCAATGA

201 ACTATATGCT GGCAACACGC CTCTTCCAGG ACAGGGGAAA CCCAAGAAGA

251 AGCTTATTGA CAGGAAGAAC ACGAATGACT GCTGATGGAG CGCCAGAATT

301 GAAGATAGAG AGTCTGAATT CAAAGGCCAA GCTGCATGCT GCACTTTACG

351 AGAGGAAGCT CCTGTCTCTG GAGGTGCGAA AACGTAGACG ACGGAGTAGC

401 AGATTGAGGG CAATGAGGCC AAAATACCCA GTGATTACCC AACCAGCTGA

451 AATGAATGTT AAAACTGAGA CAGAGAGTGA AGAGGAGGAA GAAGTCGCAT

501 TAGATAATGA AGATGAAGAA CAGGAGGCTT CCCAGGAGGA GTCTGCAGGA

551 TTTCTTAGAG AAAATCAAGC CAAATATACA CCCTCATTGA CAGCTTTGGT

601 AGAAAATACA CCCAAAGAAA ATTCCATGAA AGTTCGTGAA TGGAATAATA

651 AAGGTGGACA CTGCTGCAAA CTTGAGACTC AGGAGCTAGA GCCTAAATTT

701 AACCTGATGC AGATTCTTCA AGATAATGGC AATCTTAGCA AAATGCAGGC

751 CCGAATAGCA TTCTCTGCCT ATCTCCAGCA TGTTCAAATT CGCCTGATGA

801 AAGACAGTGG CGGTCAGACG TTCAGTGCCA GTTGGGCTGC CAAAGAGGAT

851 GAACAGATGG AGCTGGTTGT TCGTTTCCTC AAGCGAGCAT CAAATAACCT

901 CCAGCATTCA CTGAGGATGG TATTACCCAG TCGACGATTG GCACTTCTGG

951 AACGCAGAAG AATCCTGGCC CACCAGCTGG GTGACTTTAT CATTGTATAC 1001 AACAAGGAAA CAGAACAAAT GGCTGAAAAG AAATCAAAGA AGAAAGTTGA 1051 GGAAGAAGAG GAAGATGGGG TGAATATGGA AAACTTTCAG GAGTTCATCA 1101 GACAAGCAAG TGAGGCTGAA CTGGAGGAGG TGTTGACTTT TTATACCCAA 1151 AAGAACAAGT CTGCTAGTGT CTTCCTGGGG ACTCACTCTA AAATTTCTAA 1201 GAACAACAAC AATTATTCTG ATAGTGGGGC AAAAGGTGAT CACCCTGAGA 1251 CTATAATGGA AGAAGTGAAA ATAAAGCCAC CTAAACAGCA ACAGACGACA 1301 GAAATTCATT CTGATAAATT ATCTCGATTT ACCACTTCAG CAGAAAAAGA 1351 GGCAAAATTA GTTTATAGCA ATTCCTCCTC TGGTCCTACT GCTACTCTGC 1401 AGAAAATTCC CAACACCCAT TTGTCATCTG TTACAACCTC TGACCTCTCT 1451 CCAGGGCCTT GCCACCATTC TTCTTTATCT CAAATTCCTT CAGCTATCCC 1501 CAGCATGCCT CACCAGCCAA CAATTTTACT GAACACAGTC TCTGCCAGTG 1551 CTTCTCCCTG CCTACATCCC GGGGCACAGA ACATCCCAAG CCCTACTGGC 1601 CTGCCACGCT GTCGATCAGG AAGTCACACC ATTGGTCCCT TTTCTTCCTT 1651 CCAAAGTGCT GCACACATCT ATAGCCAGAA ACTGTCTCGT CCCTCTTCAG 1701 CAAAGGCAGG ATCGTGCTAT CTAAACAAGC ATCATTCAGG AATAGCCAAA 1751 ACACAAAAAG AGGGAGAAGA TGCTTCTTTA TATAGCAAAC GGTACAACCA 1801 AAGTATGGTT ACAGCTGAAC TTCAGCGGCT AGCTGAGAAG CAGGCAGCGA 1851 GACAGTATTC TCCATCCAGC CACATCAACC TCCTCACCCA ACAGGTAACA 1901 AACCTGAATT TGGCAACTGG CATCATAAAC AGAAGCAGTG CTTCAGCTCC 1951 CCCAACCCTC CGACCCATCA TCAGTCCTAG TGGCCCGACA TGGTCTACAC 2001 AGTCAGACCC CCAAGCTCCC GAGAATCACT CCAGCTCTCC TGGAAGCAGG 2051 AGCCTGCAGA CAGGGGGATT TGCCTGGGAA GGAGAAGTAG AAAACAACGT 2101 GTACAGCCAG GCTACAGGGG TGGTCCCCCA GCACAAGTAT CACCCCACAG 2151 CAGGCAGCTA TCAGCTTCAA TTTGCCCTGC AGCAACTTGA ACAACAAAAA 2201 CTTCAGTCCC GGCAGCTCCT GGACCAGAGT CGAGCCCGGC ACCAGGCAAT 2251 CTTTGGCAGC CAGACACTAC CTAACTCCAA TTTATGGACA ATGAATAATG 2301 GTGCAGGTTG TAGAATTTCC AGTGCCACAG CTAGTGGCCA GAAGCCAACC 2351 ACTCTGCCAC AAAAAGTGGT ACCACCTCCA AGTTCTTGCG CCTCCCTGGT 2401 TCCCAAACCC CCACCCAACC ACGAACAAGT GCTCAGAAGG GCAACATCCC 2451 AGAAAGCTTC CAATACCCGC TTCAGATCCT CCTTTCAAAA CTATTTGTGG 2501 TATTTCTTCC AAGCAGTCAG CTGAACTGAG GACGACAGCC TACAAACAAC 2551 TACATGCATC TGAACTGTCT CTTGTAAATG AGCTTTTTTC AGAGCCAGAA 2601 TCATACTCTC CAGGAAATAT GGAGAAAGAA ACCTGAGGAG ATTGAAGTTT 2651 GCCAGGCACA AGGGCAAAAC TCAGACTGAA TGAATTTGAA AGGGTGGGGC 2701 CAAAGATGTT GTAACCTGGG AGACTTCTCT GAAGAAAGAA AACTGTTTAA 2751 GAAACACAGA CTGAACTGCA GTACTTTTCC TTAAATAGCT GAGATGACCT 2801 TCTTTACCCT GGGCTTAGGT GATTCTCATC AGGGTGACCT GAGTGGAAGT 2851 TGGTGGTAAC GACTGTTCTG TGTCAGCACC CAGGACAGTG GTGTCTGTTA 2901 AGGCTGCCAG GGATTAGCAG GGAGGAAAGC CATCAGGACT GGGTAGCCTG 2951 GTAGCACCAA ATCCCAATTA ATGTTACCTG AACATGTGGT GAGGTCAGCC 3001 GTATGATGAA AGATGTTTAA GAGATTAATG TCAGAAGAAT ATGAAAATAA 3051 ACACCGGCTT AAAAAATGTT AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 3101 AAAAAAAAAA AAAAAAAAAA AA

BLAST Results

Entry AF107885 from database EMBL:

Homo sapiens chromosome 14q24.3 clone BAC270M14 transforming growth factor-beta 3 (TGF-beta 3) gene, complete eds; and unknown genes.

Score = 3042, P = 3.0e-219, identities = 610/612

5 exons matching 1893-3070

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 71 bp to 2521 bp; peptide length: 817 Category: strong similarity to known protein

1 MEEIKVLRRV KEENDRRGGF IRIFPTSETW EIYGSYLEHK TSMNYMLATR

51 LFQDRGNPRR SLLTGRTRMT ADGAPELKIE SLNSKAKLHA ALYERKLLSL

101 EVRKRRRRSS RLRAMRPKYP VITQPAEMNV KTETESEEEE EVALDNEDEE

151 QEASQEESAG FLRENQAKYT PSLTALVENT PKENSMKVRE WNNKGGHCCK

201 LETQELEPKF NLMQILQDNG NLSKMQARIA FSAYLQHVQI RLMKDSGGQT

251 FSASWAAKED EQMELVVRFL KRASNNLQHS LRMVLPΞRRL ALLERRRILA

301 HQLGDFIIVY NKETEQMAEK KSKKKVEEEE EDGVNMENFQ EFIRQASEAE

351 LEEVLTFYTQ KNKSASVFLG THSKISKNNN NYSDSGAKGD HPETIMEEVK

401 IKPPKQQQTT EIHSDKLSRF TTSAEKEAKL VYSNSSSGPT ATLQKIPNTH

451 LSSVTTSDLS PGPCHHSSLS QIPSAIPSMP HQPTILLNTV SASASPCLHP

501 GAQNIPSPTG LPRCRSGSHT IGPFSSFQSA AHIYSQKLΞR PSSAKAGSCY

551 LNKHHSGIAK TQKEGEDASL YSKRYNQΞMV TAELQRLAEK QAARQYSPSS

601 HINLLTQQVT NLNLATGIIN RSSASAPPTL RPIISPSGPT WSTQSDPQAP

651 ENHSSSPGSR SLQTGGFAWE GEVENNVYSQ ATGVVPQHKY HPTAGSYQLQ

701 FALQQLEQQK LQSRQLLDQS RARHQAIFGS QTLPNSNLWT MNNGAGCRIS

751 SATASGQKPT TLPQKVVPPP SSCASLVPKP PPNHEQVLRR ATSQKASNTR

801 FRSSFQNYLW YFFQAVS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_21n23, frame 2

TREMBL:AF064856_1 product: "7acomp protein"; Rattus sp. 7acomp protein mRNA, complete eds., N = 1, Score = 1845, P = 2.2e-190

TREMBL:AF107885_3 product: "unknown"; Homo sapiens chromosome 14q24.3 clone BAC270M14 transforming growth factor-beta 3 (TGF-beta 3) gene, complete eds; and unknown genes., N = 1, Score = 443, P = 5.3e-41

TREMBL:AF107885_4 product: "unknown"; Homo sapiens chromosome 14q24.3 clone BAC270M14 transforming growth factor-beta 3 (TGF-beta 3) gene, complete eds; and unknown genes., N = 1, Score = 265, P = 8.2e-22

>TREMBL : AF064856_1 product: "7acomp protein"; Rattus sp. 7acomp protein mRNA, complete eds. Length = 436

HSPs:

Score = 1845 (276.8 bits), Expect = 2.2e-190, P = 2.2e-190 Identities = 369/435 (84%), Positives = 395/435 (90%) Query: 115 MRPKYPVITQPAEMNVKTETESEEEEEVALDNEDEEQEASQEESAGFLRENQAKYTPSLT 174

MRPKYPVIT PAEMN+KTETESEEEEEV LDNEDEEQEASQEESAG L ENQAKYTPSLT Sb_jct: 1 MRPKYPVITLPAEMNIKTETESEEEEEVGLDNEDEEQEASQEESAGSLAENQAKYTPSLT 60

Query: 175 ALVENTPKENSMKVREWNNKGGHCCKLETQELEPKFNLMQILQDNGNLSKMQARIAFSAY 234

+VEN+P+EN+MKV EW NKG CCK+ETQE E KFNLMQILQDNGNLSK+QAR+AFSAY Sb_jct: 61 VIVENSPRENAMKVAEWTNKGESCCKIETQEPESKFNLMQILQDNGNLSKVQARLAFSAY 120

Query: 235 LQHVQIRLMKDSGGQTFSAΞWAAKEDEQMELVVRFLKRASNNLQHSLRMVLPSRRLALLE 294

LQHVQ+RL KDSGGQT S SWAAKEDEQMELVVRFLKRAS+NLQHSLRMVLPΞRRLALLE Sbjct: 121 LQHVQVRLTKDSGGQTLSPSWAAKEDEQMELVVRFLKRASSNLQHSLRMVLPSRRLALLE 180

Query: 295 RRRILAHQLGDFIIVYNKETEQMAEKKSKKKVEEEEEDGVNMENFQEFIRQASEAELEEV 354

RRRILAHQLGDFI+VYNKETEQMAEKKΞKKK+EEEEEDGVN E+FQEFIRQASEAELEEV Sb_jct: 181 RRRILAHQLGDFIVVYNKETEQMAEKKSKKKLEEEEEDGVNAESFQEFIRQASEAELEEV 240

Query: 355 LTFYTQKNKSASVFLGTHSKISKNNNNYSDSGAKGDHPETIMEEVKIKPPKQQQTTEIHS 414

LTFYTQKNKSASVFLGTHSK SKN+++YSDSGAKGDHPETI +EVKIK PKQQQ TEIHS Sbjct: 241 LTFYTQKNKΞASVFLGTHSKSSKNSSSYSDSGAKGDHPETI-QEVKIKQPKQQQATEIHS 299

Query: 415 DKLSRFTTSAEKEAKLVYSNSSS--GPTATL-QKIPNTHLSSV-TTSDLSPGPCHHSSLS 470

DKLSRFTTSA KEAKLVY+N SS GP A L Q++P+THLSS+ TTS LS GP HHΞSLS Sbjct: 300 DKLSRFTTSAGKEAKLVYTNCSSFSGPAAVLLQRLPSTHLSSIITTSTLSSGPGHHSSLS 359

Query: 471 QIPSAIPSMPHQPTILLNTVSASASPCLHPGAQNIPSPTGLPRCRSGSHTIGPFSSFQSA 530

QI AIPSMPHQ +LLN V SASP +HPG N+ SP GLPRCRSGS+TIGPFSSFQSA Sbjct: 360 QISPAIPSMPHQSALLLNPVPDSAΞPPVHPGTPNV-SPAGLPRCRSGSYTIGPFΞΞFQSA 418

Query: 531 AHIYSQKLSRPSSAKAG 547

AHIYSQKLSRPSSAKAG Sbjct: 419 AHIYSQKLSRPSSAKAG 435

Pedant information for DKFZphtes3_21n23, frame 2

Report for DKFZphtes3_21n23.2

[LENGTH] 817 [MW] 91522.09 [pi] 9.32 [HOMOL] TREMBL: AF064856_1 product: "7acomp protein"; Rattus sp. 7acomp protein mRNA, complete eds. le-166

[PROSITE] MYRISTYL 6

[PROSITE] CAMP_PHOSPHO_SITE 4

[PROSITE] CK2_PHOSPHO_SITE 12

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 15

[PROSITE] ASN_GLYCOSYLATION 7

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 13.83 %

SEQ MEEIKVLRRVKEENDRRGGFIRIFPTSETWEIYGSYLEHKTSMNYMLATRLFQDRGNPRR SEG PRD ccchhhhhhhhhhhccccceeeecccccceeeecceeeecccchhhhhhhhhhhcccccc

SEQ SLLTGRTRMTADGAPELKIESLNSKAKLHAALYERKLLSLEVRKRRRRSΞRLRAMRPKYP SEG xxxxxxxxxxxxxxxxxxxx PRD ccccccceeeccccceeeeehhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccc

SEQ VITQPAEMNVKTETESEEEEEVALDNEDEEQEASQEESAGFLRENQAKYTPSLTALVENT SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD ceeeccchhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhccccceeeeeccc

SEQ PKENΞMKVREWNNKGGHCCKLETQELEPKFNLMQILQDNGNLSKMQARIAFSAYLQHVQI SEG PRD cccccceeeeeccccccccchhhhhhhccchhhhhhhcccchhhhhhhhhhhhhhhhhhh

SEQ RLMKDSGGQTFSASWAAKEDEQMELVVRFLKRASNNLQHSLRMVLPSRRLALLERRRILA SEG xxxxxxxxxxxxxxx . PRD hhhhcccccceeehhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhhhh

SEQ HQLGDFIIVYNKETEQMAEKKSKKKVEEEEEDGVNMENFQEFIRQASEAELEEVLTFYTQ SEG xxxxxxxxxxxxx PRD hhccceeeeeehhhhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhhh

SEQ KNKSASVFLGTHSKISKNNNNYSDSGAKGDHPETIMEEVKIKPPKQQQTTEIHSDKLSRF SEG PRD ccccceeeecccccccccccccccccccccccchhhhhhhccccccceeeeecccccccc SEQ TTSAEKEAKLVYSNSSΞGPTATLQKIPNTHLSSVTTSDLSPGPCHHSSLSQIPSAIPSMP SEG PRD hhhhhhhheeeecccccccceeeecccccccccccccccccccccccccccccccccccc

SEQ HQPTILLNTVSASASPCLHPGAQNIPSPTGLPRCRSGSHTIGPFSSFQSAAHIYSQKLSR SEG PRD cccceeeeccccccccccccccccccccccccccccccccccccccchhhhhhhhhhccc

SEQ PSSAKAGΞCYLNKHHSGIAKTQKEGEDASLYSKRYNQSMVTAELQRLAEKQAARQYSPSS SEG PRD cccccccceeeecccccccccccccccceeeecchhhhhhhhhhhhhhhhhhhhhhcccc

SEQ HINLLTQQVTNLNLATGIINRSSASAPPTLRPIISPSGPTWSTQSDPQAPENHSSSPGSR SEG .. xxxxxxxxxxxx PRD cccccccccccccccccccccccccccccceeeecccccccccccccccccccccccccc

SEQ SLQTGGFAWEGEVENNVYSQATGVVPQHKYHPTAGSYQLQFALQQLEQQKLQSRQLLDQS SEG xxxxxxxxxxxxxxxxxxx ... PRD cccccccceeeeecccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhh

SEQ RARHQAIFGSQTLPNSNLWTMNNGAGCRISSATASGQKPTTLPQKVVPPPΞSCASLVPKP SEG PRD hhhhhhhhccccccccceeeeccccceeeeeeeccccccccccceeecccccceeecccc

SEQ PPNHEQVLRRATSQKASNTRFRSSFQNYLWYFFQAVS SEG PRD cccchhhhhhhhhhhcccccccccccceeeeeeeccc

Prosite for DKFZphtes3_21n23.2

PS00001 221->225 ASNJ3LYCOSYLATION PDOC00001 PS00001 362->366 ASN_GLYCOSYLATION PDOC00001 PS00001 381->385 ASN_GLYCOSYLATION PDOC00001 PS00001 434->438 ASN_GLYCOSYLATION PDOC00001 PS00001 576->580 ASN_GLYCOSYLATION PDOC00001 PS00001 620->624 ASN_GLYCOSYLATION PDOC00001 PS00001 652->656 ASN_GLYCOSYLATION PDOC00001 PS00004 106->110 CAMP_PHOSPHO_SITE PDOC00004 PS00004 107->111 CAMP_PHOSPHO_SITE PDOC00004 PS00004 271->275 CAMP_PHOSPHO_SITE PDOC00004 PS00004 789->793 CAMP_PHOSPHO_SITE PDOC00004 PS00005 64->67 PKC_PHOSPHO_SITE PDOC00005 PS00005 109-M12 PKC_PHOSPHO_SITE PDOC00005 PS00005 180->183 PKC_PHOSPHO_SITE PDOC00005 PS00005 185->188 PKC_PHOSPHO_SITE PDOC00005 PS00005 280->283 PKC_PHOSPHO_SITE PDOC00005 PS00005 287->290 PKC_PHOSPHO_SITE PDOC00005 PS00005 322->325 PKC_PHOSPHO_SITE PDOC00005 PS00005 359->362 PKC_PHOSPHO_SITE PDOC00005 PS00005 414->417 PKC_PHOSPHO_SITE PDOC00005 PS00005 535->538 PKC_PHOSPHO_SITE PDOC00005 PS00005 543->546 PKC_PHOSPHO_SITE PDOC00005 PS00005 561->564 PKC_PHOSPHO_SITE PDOC00005 PS00005 572->575 PKC_PHOSPHO_SITE PDOC00005 PS00005 629->632 PKC_PHOSPHO_SITE PDOC00005 PS00005 793->796 PKC_PHOSPHO_SITE PDOC00005 PS00006 35->39 CK2_PHOSPHO_SITE PDOC00006 PS00006 132->136 CK2_PHOSPHO_SITE PDOC00006 PS00006 134->138 CK2_PHOSPHO_SITE PDOC00006 PS00006 136->140 CK2_PHOSPHO_SITE PDOC00006 PS00006 154->158 CK2_PHOSPHO_SITE PDOC00006 PS00006 180->184 CK2_PHOSPHO_SITE PDOC00006 PS00006 347->351 CK2_PHOSPHO_SITE PDOC00006 PS00006 394->398 CK2_PHOSPHO_SITE PDOC00006 PS00006 422->426 CK2_PHOΞPHO_SITE PDOC00006 PS00006 455->459 CK2_PHOSPHO_SITE PDOC00006 PS00006 561->565 CK2_PHOSPHO_SITE PDOC00006 PS00006 643->647 CK2_PHOSPHO_SITE PDOC00006 PS00007 563->572 TYR_PHOSPHO_SITE PDOC00007 PS00008 195->201 MYRISTYL PDOC00008 PS00008 248->254 MYRISTYL PDOC00008 PS00008 510->516 MYRISTYL PDOC00008 PS00008 557->563 MYRISTYL PDOC00008 PS00008 746->752 MYRISTYL PDOC00008 PS00008 756->762 MYRISTYL PDOC00008

(No Pfam data available for DKFZphtes3_21n23.2) DKFZphtes3_22c23

group: testes derived

DKFZphtes3_22c23 encodes a novel 223 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, 3 EST hits (two from a testis library)

Sequenced by LMU

Locus: /map="9q34"

Insert length: 1113 bp

Poly A stretch at pos. 1073, polyadenylation signal at pos. 1055

1 GGTGGGCAAA GGCATCTTCC TCTGGGAAGG ACTGGCACAA GCACTTGGTC 51 CCTGGGTTGT GTGCCTGGGA GGCCGGGATC AGGGCTGGCC CTCTTTCTCC

101 CTGGCAAAGC AAAACCTCCC TTTTACTACT ATCAAGGGGA AGTAACTTGA

151 AGGTGCCTGT GGCAGGCAGC ACCTTGAGCC AACAGGAACC ATTGACATGC

201 GAGGCCCAGG GCAGGCAGAC TGTGCAGTGG CCATTGGGCG GCCCCTCGGG

251 GAGGTGGTGA CCCTCCGCGT CCTTGAGAGT TCTCTCAACT GCAGTGCGGG

301 GGACATGTTG CTGCTTTGGG GCCGGCTCAC CTGGAGGAAG ATGTGCAGGA

351 AGCTGTTGGA CATGACTTTC AGCTCCAAGA CCAACACGCT GGTGGTGAGG

401 CAGCGCTGCG GGCGGCCAGG AGGTGGGGTG CTGCTGCGGT ATGGGAGCCA

451 GCTTGCTCCT GAAACCTTCT ACAGAGAATG TGACATGCAG CTCTTTGGGC

501 CCTGGGGTGA AATCGTGAGC CCCTCGCTGA GTCCAGCCAC GAGTAATGCA

551 GGGGGCTGCC GGCTCTTCAT TAATGTGGCT CCGCACGCAC GGATTGCCAT

601 CCATGCCCTG GCCACCAACA TGGGCGCTGG GACCGAGGGA GCCAATGCCA

651 GCTACATCTT GATCCGGGAC ACCCACAGCT TGAGGACCAC AGCGTTCCAT

701 GGGCAGCAGG TGCTCTACTG GGAGTCAGAG AGCAGCCAGG CTGAGATGGA

751 GTTCAGCGAG GGCTTCCTGA AGGCTCAGGC CAGCCTGCGG GGCCAGTACT

801 GGACCCTCCA ATCATGGGTA CCGGAGATGC AGGACCCTCA GTCCTGGAAG

851 GGAAAGGAAG GAACCTGAGG GTCATTGAAC ATTTGTTCCG TGTCTGGCCA

901 GCCCTGGAGG GTTGACCCCT GGTCTCAGTG CTTTCCAATT CGAACTTTTT

951 CCAATCTTAG GTATCTACTT TAGAGTCTTC TCCAATGTCC AAAAGGCTAG 1001 GGGGTTGGAG GTGGGGACTC TGGAAAAGCA GCCCCCATTT CCTCGGGTAC 1051 CAATAAATAA AACATGCAGG CTGAAAAAAA AAAAAAAAAA AAAAAAAAAA 1101 AAAAAAAAAA AAA

BLAST Results

Entry HSAC1644 from database EMBL:

Genomic sequence from Human 9q34, complete sequence. Score = 2072, P = 8.8e-225, identities = 422/430 5 exons Bp 41969-38232

Medline entries

No Medlme entry

Peptide information for frame 2

ORF from 197 bp to 865 bp; peptide length: 223 Category: putative protein

1 MRGPGQADCA VAIGRPLGEV VTLRVLESSL NCSAGDMLLL WGRLTWRKMC

51 RKLLDMTFSS KTNTLVVRQR CGRPGGGVLL RYGSQLAPET FYRECDMQLF

101 GPWGEIVSPS LSPATSNAGG CRLFINVAPH ARIAIHALAT NMGAGTEGAN

151 ASYILIRDTH SLRTTAFHGQ QVLYWESESS QAEMEFSEGF LKAQASLRGQ

201 YWTLQSWVPE MQDPQSWKGK EGT BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_22c23, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphtes3_22c23, frame 2

Report for DKFZphtes3_22c23.2

[LENGTH] 223

[MW] 24546.19

[pi] 8.57

[PROSITE] MYRISTYL 4

[PROSITE] CK2_PHOΞPHO_SITE

[PROSITE] PKC_PHOSPHO_SI E

[PROSITE] ASN_GLYCOSYLATION

[KW] Alpha_Beta

SEQ MRGPGQADCAVAIGRPLGEVVTLRVLESSLNCSAGDMLLLWGRLTWRKMCRKLLDMTFSS PRD ccccccccceeeecccccceeeeehhhhhcccccchhhhhhchhhhhhhhhhhhhhhccc

SEQ KTNTLVVRQRCGRPGGGVLLRYGSQLAPETFYRECDMQLFGPWGEIVSPSLSPATSNAGG PRD ccceeeeeecccccccceeeeccccccchhhhhhhhhccccccceeeecccccccccccc

SEQ CRLFINVAPHARIAIHALATNMGAGTEGANASYILIRDTHSLRTTAFHGQQVLYWESESS PRD ceeeeeecccceeehhhhhhhhccccccccceeeeeecccccceeecccceeeeeccccc

SEQ QAEMEFSEGFLKAQASLRGQYWTLQSWVPEMQDPQSWKGKEGT PRD hhhhhhhcchhhhhhhhhhcccccccccccccccccccccccc

Prosite for DKFZphtes3_22c23.2

PS00001 31->35 ASN_GLYCOSYLATION PDOC00001 PS00001 150->154 ASN_GLYCOSYLATION PDOC00001 PS00005 22->25 PKC_PHOSPHO_SITE PDOC00005 PS00005 45->48 PKC_PHOSPHO_SITE PDOC00005 PS00005 59->62 PKC_PHOSPHO_SITE PDOC00005 PS00005 161->164 PKC_PHOSPHO_SITE PDOC00005 PS00005 19θ->199 PKC_PHOSPHO_SITE PDOC00005 PS00005 216->219 PKC_PHOSPHO_SITE PDOC00005 PS00006 33->37 CK2_PHOSPHO_SITE PDOC00006 PS00006 180->184 CK2_PHOSPHO_SITE PDOC00006 PS00008 5->ll MYRISTYL PDOC00008 PS00008 145->151 MYRISTYL PDOC00008 PS00008 148->154 MYRISTYL PDOC00008 PS00008 199->205 MYRISTYL PDOC00008

(No Pfam data available for DKFZphtes3_22c23.2)

DKFZphtes3_22g2

group: nucleic acid management

DKFZphtes3_22g2encodes a novel 1230 ammo acid protein with nearly identical to rat TIP120.

TATA-binding protein TBP is a central component for transcriptional regulation and is a target for various transcription regulators. TBP-interacting protein 120 (TIP120) is a protein interacting with the TATA-binding protein (TBP) . The novel protein is the human ortholog of rat TIP120. The novel TBP-binding protein is considered to participate in transcription regulation through the interaction with TBP.

The new protein can find application m modulation of gene transcription.

KIAA0829, complete eds, nearly identical to rat TIP120 complete cDNA, complete eds, EST hits,

Sequenced by LMU

Locus: /map="387.3 cR from top of Chrl2 linkage group"

Insert length: 5387 bp

Poly A stretch at pos. 5352, polyadenylation signal at pos. 5335

1 GGGAGCGAGT GCGGAGCGAG TGGGAGCGAG ACGGCCCTGA GTGGAAGTGT

51 CTGGCTCCCC GTAGAGGCCC TTCTGTACGC CCCGCCGCCC ATGAGCTCGT

101 TCTCACGCGA ACAGCGCCGT CGTTAGGCTG GCTCTGTAGC CTCGGCTTAC

151 CCCGGGACAG GCCCACGCCT CGCCAGGGAG GGGGCAGCCC GTCGAGGCGC

201 CTCCCTAGTC AGCGTCGGCG TCGCGCTGCG ACCCTGGAAG CGGGAGCCGC

251 CGCGAGCGAG AGGAGGAGCT CCAGTGGCGG CGGCGGCGGC GGCAGCGGCA

301 GCGGGCAGCA GCTCCAGCAG CGCCAGCAGG CGGGATCGAG GCCGTCAACA

351 TGGCGAGCGC CTCGTACCAC ATTTCCAATT TGCTGGAAAA AATGACATCC

401 AGCGACAAGG ACTTTAGGTT TATGGCTACA AATGATTTGA TGACGGAACT

451 GCAGAAAGAT TCCATCAAGT TGGATGATGA TAGTGAAAGG AAAGTAGTGA

501 AAATGATTTT GAAGTTATTG GAAGATAAAA ATGGAGAGGT ACAGAATTTA

551 GCTGTCAAAT GTCTTGGTCC TTTAGTGAGT AAAGTGAAAG AATACCAAGT

601 AGAGACAATT GTAGATACCC TCTGCACTAA CATGCTTTCT GATAAAGAAC

651 AACTTCGAGA CATTTCAAGT ATTGGTCTTA AAACAGTAAT TGGAGAACTT

701 CCTCCAGCTT CCAGTGGCTC TGCATTAGCT GCTAATGTAT GTAAAAAGAT

751 TACTGGACGT CTTACAAGTG CAATAGCAAA ACAGGAAGAT GTCTCTGTTC

801 AGCTAGAAGC CTTGGATATT ATGGCTGATA TGTTGAGCAG GCAAGGAGGA

851 CTTCTTGTTA ATTTCCATCC TTCAATTCTG ACCTGTCTAC TTCCCCAGTT

901 GACCAGCCCT AGACTTGCAG TGAGGAAAAG AACCATTATC GCTCTTGGCC

951 ATCTGGTTAT GAGCTGTGGA AATATAGTTT TTGTAGATCT TATTGAACAT

1001 CTGTTGTCAG AGTTGTCCAA AAATGATTCT ATGTCAACAA CAAGAACCTA

1051 CATACAATGT ATTGCTGCTA TTAGTAGGCA AGCTGGTCAT AGAATAGGTG

1101 AATACCTTGA GAAGATAATT CCTTTGGTGG TAAAATTTTG CAATGTAGAT

1151 GATGATGAAT TAAGAGAGTA CTGTATTCAA GCCTTTGAAT CATTTGTAAG

1201 AAGATGTCCT AAGGAAGTAT ATCCTCATGT TTCTACCATT ATAAATATTT

1251 GTCTTAAATA TCTTACCTAT GATCCAAATT ATAATTACGA TGATGAAGAT

1301 GAAGATGAAA ATGCAATGGA TGCTGATGGT GGTGATGATG ATGATCAAGG

1351 GAGTGATGAT GAATACAGTG ATGATGATGA CATGAGTTGG AAAGTGAGAC

1401 GTGCAGCTGC GAAGTGCTTG GATGCTGTAG TTAGCACAAG GCATGAAATG

1451 CTTCCAGAAT TCTACAAGAC CGTCTCTCCT GCACTAATAT CCAGATTTAA

1501 AGAGCGTGAA GAGAATGTAA AGGCAGATGT TTTTCACGCA TACCTTTCTC

1551 TTTTGAAGCA AACTCGTCCT GTACAAAGTT GGCTATGTGA CCCTGATGCA

1601 ATGGAGCAGG GAGAAACACC TTTAACAATG CTTCAGAGTC AGGTTCCCAA

1651 CATTGTTAAA GCTCTTCACA AACAGATGAA AGAAAAAAGT GTGAAGACCC

1701 GACAGTGTTG TTTTAACATG TTAACTGAGC TGGTAAATGT ATTACCTGGG

1751 GCCCTAACTC AACACATTCC TGTACTTGTA CCAGGAATCA TTTTCTCACT

1801 GAATGATAAA TCAAGCTCAT CGAATTTGAA GATCGATGCT TTGTCATGTC

1851 TATACGTAAT CCTCTGTAAC CATTCTCCTC AAGTCTTCCA TCCTCACGTT

1901 CAGGCTTTGG TTCCTCCAGT GGTGGCTTGT GTTGGAGACC CATTTTACAA

1951 AATTACATCT GAAGCACTTC TTGTTACTCA ACAGCTTGTC AAAGTAATTC

2001 GTCCTTTAGA TCAGCCTTCC TCGTTTGATG CAACTCCTTA TATCAAAGAT

2051 CTATTTACCT GTACCATTAA GAGATTAAAA GCAGCTGACA TTGATCAGGA

2101 AGTCAAGGAA AGGGCTATTT CCTGTATGGG ACAAATTATT TGCAACCTTG

2151 GAGACAATTT GGGTTCTGAC TTGCCTAATA CACTTCAGAT TTTCTTGGAG

2201 AGACTAAAGA ATGAAATTAC CAGGTTAACT ACAGTAAAGG CATTGACACT

2251 GATTGCTGGG TCACCTTTGA AGATAGATTT GAGGCCTGTT CTGGGAGAAG

2301 GGGTTCCTAT CCTTGCTTCA TTTCTTAGAA AAAACCAGAG AGCTTTGAAA

2351 CTGGGTACTC TTTCTGCCCT TGATATTCTA ATAAAAAACT ATAGTGACAG

2401 CTTGACAGCT GCCATGATTG ATGCAGTTCT AGATGAGCTC CCACCTCTTA

2451 TCAGCGAAAG TGATATGCAT GTTTCACAAA TGGCCATCAG TTTTCTTACC

2501 ACTTTGGCAA AAGTATATCC CTCCTCCCTT TCAAAGATAA GTGGATCCAT

2551 TCTCAATGAA CTTATTGGAC TTGTGAGATC ACCCTTATTG CAGGGGGGAG 2601 CTCTTAGTGC CATGCTAGAC TTTTTCCAAG CTCTGGTTGT CACTGGAACA

2651 AATAATTTAG GATACATGGA TTTGTTGCGC ATGCTGACTG GTCCAGTTTA 2701 CTCTCAGAGC ACAGCTCTTA CTCATAAGCA GTCTTATTAT TCCATTGCCA 2751 AATGTGTAGC TGCCCTTACT CGAGCATGCC CTAAAGAGGG ACCAGCTGTA 2801 GTAGGTCAGT TTATTCAAGA TGTCAAGAAC TCAAGGTCTA CAGATTCCAT 2851 TCGTCTCTTA GCTCTACTTT CTCTTGGAGA AGTTGGGCAT CATATTGACT 2901 TAAGTGGACA GTTGGAACTA AAATCTGTAA TACTAGAAGC TTTCTCATCT 2951 CCTAGTGAAG AAGTCAAATC AGCTGCATCC TATGCATTAG GCAGCATTAG 3001 TGTGGGCAAC CTTCCTGAAT ATCTGCCGTT TGTCCTGCAA GAAATAACTA 3051 GTCAACCCAA AAGGCAGTAT CTTTTACTTC ATTCCTTGAA GGAAATTATT 3101 AGCTCTGCAT CAGTGGTGGG CCTTAAACCA TATGTTGAAA ACATCTGGGC 3151 CTTATTACTA AAGCACTGTG AGTGTGCAGA GGAAGGAACC AGAAATGTTG 3201 TTGCTGAATG TCTAGGAAAA CTCACTCTAA TTGATCCAGA AACTCTCCTT 3251 CCACGGCTTA AGGGGTACTT GATATCAGGC TCATCATATG CCCGAAGCTC 3301 AGTGGTTACG GCTGTGAAAT TTACAATTTC TGACCATCCA CAACCTATTG 3351 ATCCACTGTT AAAGAACTGC ATAGGTGATT TCCTAAAAAC TTTGGAAGAC 3401 CCAGATTTGA ATGTGAGAAG AGTAGCCTTG GTCACATTTA ATTCAGCAGC 3451 ACATAACAAG CCATCATTAA TAAGGGATCT ATTGGATACT GTTCTTCCAC 3501 ATCTTTACAA TGAAACAAAA GTTAGAAAGG AGCTTATAAG AGAGGTAGAA 3551 ATGGGTCCAT TTAAACATAC GGTTGATGAT GGTCTGGATA TTAGAAAGGC

3601 AGCATTTGAG TGTATGTACA CACTTCTAGA CAGTTGTCTT GATAGACTTG 3651 ATATCTTTGA ATTTCTAAAT CATGTTGAAG ATGGTTTGAA GGACCATTAT 3701 GATATTAAGA TGCTGACATT TTTAATGTTG GTGAGACTGT CTACCCTTTG 3751 TCCAAGTGCA GTACTGCAGA GGTTGGACCG ACTTGTTGAG CCATTACGTG 3801 CAACATGTAC AACTAAGGTA AAGGCAAACT CAGTAAAGCA GGAGTTTGAA 3851 AAACAAGATG AATTAAAGCG ATCTGCCATG AGAGCAGTAG CAGCACTGCT 3901 AACCATTCCA GAAGCAGAGA AGAGTCCACT GATGAGTGAA TTCCAGTCAC 3951 AGATCAGTTC TAACCCTGAG CTGGCGGCTA TCTTTGAAAG TATCCAGAAA 4001 GATTCATCAT CTACTAACTT GGAATCAATG GACACTAGTT AGATGTTTGT 4051 TCACCATGGG GACCATTACA TATGACCATA CAATGCACTG AATTGACAGG 4101 TTAATCATAA GACATGGAAA GAGAAGTGTC TAAAAGCTTC AAAATGTTCC 4151 ACTTTTTTTT CCTTCATGGA GACTGTTTGT TTGGCTTTCT TCCATTGTTG 4201 TTTTTGTAGC ATTTATTTCA GAAATGTGTA TTTCCATAAT CCAGAGGTTG 4251 TAAAACCACT AGTGTTTTAG TGGTTACAGC AACATTTGAA ATGGAAACTA 4301 AAAGTTAGGA TTTTATGGAG TATGGAGATA GGGTCCAGTA TCTATTTACC 4351 CTGTAATGTT TAGGATTAAA ATGTTAAAAT TTTGTGACCA TGAATTTCTT

4401 TCTTTTATAA ATTTTCTCAT TTAAAAATCA AAAATCTTGC AAAACAAAAA 4451 CCATGTTTCT TTTTCTTGTA TAACTTTTTG TTTTCAGCAA CATAAATTGA 4501 TTTTTAGCTG GCAGACAAGA ATATCCATAT AAGATTTGTT AACCATTTCA 4551 GAGAGTTTGG CAATTTTTAA AAGATAATAA GGTATCATTT TTAAGTATGA 4601 AAATTAACAA TATCCCTGTT GCGCACACTA ATTTTGCATG AGTAAGTTTA 4651 CAAATATGTA TCGTCTGTAA AGCAGCATGT GCAGATTATT CATAATATAG 4701 AAGTTAAAAT AAGTATTAGT GCAATTTTCA GATATTTATT TTTGCACAGA 4751 AAACACATTA TCTGGAGAGA AAGAAAGGAG AATTTTTGAG ACTTGGGTTT 4801 TCTTAATGCC AGTGTGAATT TGCAGATGTT TTCAGAAAAT CAAGTCACAG 4851 TAACAATTTG CCACTTTTTT CTATTATAAA TCTTCTTACT TAAATTTTGA 4901 ATATTTAGTT TTTCTCAGTT ACCCATTTGT GTGTGTGTGA TTCCACTTAG 4951 AAATTCTTAA AACCAGATTT TTCTTTCATT CCGTTTGGAT GTCTACATTC 5001 CTTATCAAAG GATATAAATA CTGTGTATGC TTTTGAATTT TATTTTTAGG 5051 AAAATTCTGA AGCCAGCTAT CACAGGTTTG TTAGCTAATA ATAGTATTTT 5101 CTTTTAGTTG AGTTAGGTTT TTCCCCATCT CCTGTAGAGC GAATTTACAT 5151 ATTGTATTGG GTAAGTGTTC ACTACTTTTC CTGATTAAGG GATCTGTGCT 5201 GGGGAACAAA GCTTTTGCAG TACCTTATAT TGTAGTTAAA ATTTTATTTA 5251 ACATATCCTT CAGTGAGCTC ATTTCACACT GTAGCCTCTT CCTTAAAATT 5301 TGTGGTGCTC CTGTAACAGT AAGAACTAAT TCTGAAATAA AAGACATCTC 5351 CTAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAA

BLAST Results

Entry HS793345 from database EMBL: human STS WI-12457. Score = 1985, P = 1.3e-83, identities = 433/460

Medline entries

97127450:

Molecular cloning of a novel 120-kDa TBP-mteracting protein.

Peptide information for frame 2 ORF from 350 bp to 4039 bp; peptide length: 1230 Category: known protein Classification: Nucleic acid management

1 MASASYHISN LLEKMTSSDK DFRFMATNDL MTELQKDSIK LDDDΞERKVV

51 KMILKLLEDK NGEVQNLAVK CLGPLVSKVK EYQVETIVDT LCTNMLSDKE

101 QLRDISSIGL KTVIGELPPA SSGSALAANV CKKITGRLTS AIAKQEDVSV

151 QLEALDIMAD MLSRQGGLLV NFHPSILTCL LPQLTSPRLA VRKRTIIALG

201 HLVMSCGNIV FVDLIEHLLS ELSKNDSMST TRTYIQCIAA ISRQAGHRIG

251 EYLEKIIPLV VKFCNVDDDE LREYCIQAFE SFVRRCPKEV YPHVSTIINI

301 CLKYLTYDPN YNYDDEDEDE NAMDADGGDD DDQGΞDDEYS DDDDMSWKVR

351 RAAAKCLDAV VSTRHEMLPE FYKTVSPALI SRFKEREENV KADVFHAYLS

401 LLKQTRPVQS WLCDPDAMEQ GETPLTMLQS QVPNIVKALH KQMKEKSVKT

451 RQCCFNMLTE LVNVLPGALT QHIPVLVPGI IFSLNDKSSΞ SNLKIDALSC

501 LYVILCNHSP QVFHPHVQAL VPPVVACVGD PFYKITSEAL LVTQQLVKVI

551 RPLDQPSSFD ATPYIKDLFT CTIKRLKAAD IDQEVKERAI SCMGQIICNL

601 GDNLGSDLPN TLQIFLERLK NEITRLTTVK ALTLIAGSPL KIDLRPVLGE

651 GVPILASFLR KNQRALKLGT LSALDILIKN YSDSLTAAMI DAVLDELPPL

701 ISESDMHVSQ MAISFLTTLA KVYPΞSLSKI SGSILNELIG LVRSPLLQGG

751 ALSAMLDFFQ ALVVTGTNNL GYMDLLRMLT GPVYSQSTAL THKQSYYSIA

801 KCVAALTRAC PKEGPAVVGQ FIQDVKNSRS TDΞIRLLALL SLGEVGHHID

851 LSGQLELKSV ILEAFSSPSE EVKSAASYAL GSISVGNLPE YLPFVLQEIT

901 SQPKRQYLLL HSLKEIISSA SVVGLKPYVE NIWALLLKHC ECAEEGTRNV

951 VAECLGKLTL IDPETLLPRL KGYLISGSSY ARSSVVTAVK FTISDHPQPI

1001 DPLLKNCIGD FLKTLEDPDL NVRRVALVTF NSAAHNKPSL IRDLLDTVLP

1051 HLYNETKVRK ELIREVEMGP FKHTVDDGLD IRKAAFECMY TLLDSCLDRL

1101 DIFEFLNHVE DGLKDHYDIK MLTFLMLVRL STLCPSAVLQ RLDRLVEPLR

1151 ATCTTKVKAN SVKQEFEKQD ELKRΞAMRAV AALLTIPEAE KSPLMSEFQS

1201 QISSNPELAA IFESIQKDSΞ STNLESMDTS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_22g2, frame 2

TREMBL:AB020636_1 gene: "KIAA0829"; product: "KIAA0829 protein"; Homo sapiens mRNA for KIAA0829 protein, partial eds., N = 1, Score = 5986, P = 0

TREMBL :RND6711_1 gene: "tιpl20"; product: "TIP120"; Rattus norvegicus mRNA for TIP120, complete eds., N = 1, Score = 6203, P = 0

>TREMBL:RND6711_1 gene: "tιpl20"; product: "TIP120"; Rattus norvegicus mRNA for TIP120, complete eds. Length = 1,230

HSPs:

Score = 6203 (930.7 bits), Expect = O.Oe+00, P = O.Oe+00 Identities = 1227/1230 (99%), Positives = 1228/1230 (99%)

Query: 1 MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDK 60

MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDK Sbjct: 1 MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDK 60

Query: 61 NGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPA 120

NGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLΞDKEQLRDISSIGLKTVIGELPPA Sbjct: 61 NGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPA 120

Query: 121 SSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCL 180

SSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCL Sbjct: 121 SSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCL 180

Query: 181 LPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAA 240

LPQLTSPRLAVRKRTIIALGHLVMΞCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAA Sbjct: 181 LPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAA 240

Query: 241 ISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFEΞFVRRCPKEVYPHVSTIINI 300

ISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFEΞFVRRCPKEVYPHVSTIINI Sbjct: 241 ISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINI 300

Query: 301 CLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAV 360

CLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAV Sbjct: 301 CLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAV 360

Query: 361 VSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQ 420

VSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQ Sb_jct: 361 VSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQ 420 Query: 421 GETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGI 480

GETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGI Sbjct: 421 GETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGI 480

Query: 481 IFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEAL 540

IFSLNDKSSΞSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEAL Sbjct: 481 IFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEAL 540

Query: 541 LVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNL 600

LVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNL Sbjct: 541 LVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAIΞCMGQIICNL 600

Query: 601 GDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLR 660

GDNLG DL NTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLR Sbjct: 601 GDNLGPDLSNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLR 660

Query: 661 KNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLA 720

KNQRALKLGTLSALDILIKNYΞDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLA Sbjct: 661 KNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISEΞDMHVSQMAISFLTTLA 720

Query: 721 KVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLT 780

KVYPSΞLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLT Sbjct: 721 KVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLT 780

Query: 781 GPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALL 840

GPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALL Sbjct: 781 GPVYSQΞTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALL 840

Query: 841 SLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEIT 900

SLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEIT Sbjct: 841 SLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEIT 900

Query: 901 SQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTL 960

SQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTL Sbjct: 901 SQPKRQYLLLHSLKEI ISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTL 960

Query: 961 IDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDL 1020

IDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDL Sbjct: 961 IDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDL 1020

Query: 1021 NVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLD 1080

NVRRVALVTFNSAAHNKPSLIRDLLD+VLPHLYNETKVRKELIREVEMGPFKHTVDDGLD Sbjct: 1021 NVRRVALVTFNSAAHNKPSLIRDLLDSVLPHLYNETKVRKELIREVEMGPFKHTVDDGLD 1080

Query: 1081 IRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLΞTLCPSAVLQ 1140

IRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQ Sbjct: 1081 IRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQ 1140

Query: 1141 RLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQS 1200

RLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTI PEAEKSPLMSEFQS Sbjct: 1141 RLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQS 1200

Query: 1201 QISSNPELAAIFESIQKDSSSTNLESMDTS 1230

QISSNPELAAIFESIQKDSSSTNLESMDTS Sbjct: 1201 QISSNPELAAIFESIQKDSSSTNLESMDTS 1230

Pedant information for DKFZphtes3_22g2, frame 2

Report for DKFZphtes3_22g2.2

[LENGTH] 1230

[MW] 136376.58

[pi] 5.52

[HOMOL] TREMBL :RND6711_1 gene: "tιpl20"; product: "TIP120"; Rattus norvegicus mRNA for

TIP120, complete eds. 0.0

[KW] TRANSMEMBRANE 1

[KW] LOW_COMPLEXITY 5.28 %

SEQ MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDK

SEG

PRD cccccchhhhhhhhhcccccceeeeehhhhhhhhhcccccccccchhhhhhhhhhhhhcc

MEM

SEQ NGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPA

SEG xxxx

PRD ccccceeeeeeeeceeeeehhhhhhhhhhhhccchhhhhcccccccchhhhhhhhhcccc MEM

SEQ SSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCL

SEG xxxxxxxx

PRD cccccchhhhhhhccchhhhhhhccccchhhhhhhhhhhhhhhhhccceeeecchhhhhh

MEM

SEQ LPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAA

SEG

PRD hcccccchhhhhhhhhhhheeeeecccceeehhhhhhhhhhhhccccchhhhhhhhhhhh

MEM MMMMMMMMMMMMMMMMM

SEQ ISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINI

SEG

PRD hhhhcccccccchhhhhhhhheeeeccchhhhhhhhhhhhhhhhccccceeecchhhhhh

MEM

SEQ CLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAV

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD hhhhhccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhh

MEM

SEQ VSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQ

SEG

PRD hhhhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhhhccceeeecccccccc

MEM

SEQ GETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGI

SEG

PRD cccchhhhhhhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhccccccccceeeecce

MEM

SEQ IFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEAL

SEG xxxxxxxxxxxxxxxx

PRD eeeeccccccccchhhhhhhheeeeecccccccccceeeeecceeeeecccchhhhhhhh

MEM

SEQ LVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNL

SEG

PRD hhhhhhhhhhcccccccccccccchhhhhhhhhhhhhhccchhhhhhhhhhhhheeeecc

MEM

SEQ GDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLR

SEG

PRD cccccccccchhhhhhhhhcchhhhhhhhhhhheeeeccccccccceeehhhhhhhhhhh

MEM

SEQ KNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLA

SEG

PRD hhhhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhcccccccchhhhhhhhhhhhhhhh

MEM

SEQ KVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLT

SEG

PRD cccccceeecchhhhhhhhhhhccccccchhhhhhhhhhhheeeecccccchhhhhhhhc

MEM

SEQ GPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALL

SEG

PRD cccccccccchhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhcccccchhhhhhhh

MEM

SEQ SLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEIT

SEG

PRD hccccccccccccccccceeeeeeccccchhhhhhhhhhhccccccccccchhhhhhhhh

MEM

SEQ SQPKRQYLLLHSLKEIISSAΞVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTL

SEG

PRD cccchhhhhhhhhhhhhhcccceeehhhhhhhhhhhhhhhhcccccceeeeecccccccc

MEM

SEQ IDPETLLPRLKGYLISGSSYARSSVVTAVKFTIΞDHPQPIDPLLKNCIGDFLKTLEDPDL

SEG

PRD cccccccccccccccccccccchhhhhhhhhhhccccccccccchhhhhhhhhhhccccc

MEM

SEQ NVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLD

SEG

PRD ccceeeeeeecccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccch

MEM SEQ IRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQ

SEG

PRD hhhhhhhhhhhhhhhccccccceeeecccccccccchhhhhhhhhhhhhhhhcccchhhh

MEM

SEQ RLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQS

SEG

PRD hhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccchhhhh

MEM

SEQ QISSNPELAAIFESIQKDSSSTNLESMDTS

SEG

PRD hhhccchhhhhhhhhhhccccccccccccc

MEM

(No Prosite data available for DKFZphtes3_22g2.2) (No Pfam data available for DKFZphtes3_22g2.2)

DKFZphtes3_22nl3

group: testes derived

DKFZphtes3_22nl3 encodes a novel 677 ammo acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . dJ1042K10.3, complete

Sequenced by LMU

Locus: /map="22ql3.1-13.2"

Insert length: 3353 bp

Poly A stretch at pos. 3315, polyadenylation signal at pos. 3298

1 ATGGAACCAC TATCCCCACT GCCAAGTCCA CCCCCACACT CATTAAGCAA

51 AGCCAACCCA AGTCTGCCAG TGAGAAGTCA CAGCGCAGCA AGAAGGCCAA

101 GGAGCTGAAG CCAAAGGTGA AGAAGCTCAA GTACCACCAG TACATCCCCC

151 CGGACCAGAA GCAGGACAGG GGGGCACCCC CCATGGACTC ATCCTACGCC

201 AAGATCCTGC AGCAGCAGCA GCTCTTCCTC CAGCTGCAGA TCCTCAACCA

251 GCAGCAGCAG CAGCACCACA ACTACCAGGC CATCCTGCCT GCCCCGCCAA

301 AGTCAGCAGG CGAGGCCCTG GGAAGCAGCG GGACCCCCCC AGTACGCAGC

351 CTCTCCACTA CCAATAGCAG CTCCAGCTCG GGCGCCCCTG GGCCCTGTGG

401 GCTGGCACGT CAGAACAGCA CCTCACTGAC TGGCAAGCCG GGAGCCCTGC

451 CGGCCAACCT GGACGACATG AAGGTGGCAG AGCTGAAGCA GGAGCTGAAG

501 TTGCGATCAC TGCCTGTCTC GGGCACCAAA ACTGAGCTGA TTGAGCGCCT

551 TCGAGCCTAT CAAGACCAAA TCAGCCCTGT GCCAGGAGCC CCCAAGGCCC

601 CTGCCGCCAC CTCTATCCTG CACAAGGCTG GCGAGGTGGT GGTAGCCTTC

651 CCAGCGGCCC GGCTGAGCAC GGGGCCAGCC CTGGTGGCAG CAGGCCTGGC

701 TCCAGCTGAG GTGGTGGTGG CCACGGTGGC CAGCAGTGGG GTGGTGAAGT

751 TTGGCAGCAC GGGCTCCACG CCCCCCGTGT CTCCCACCCC CTCGGAGCGC

801 TCACTGCTCA GCACGGGCGA TGAAAACTCC ACCCCCGGGG ACACCTTTGG

851 TGAGATGGTG ACATCACCTC TGACGCAGCT GACCCTGCAG GCCTCGCCAC

901 TGCAGATCCT CGTGAAGGAG GAGGGCCCCC GGGCCGGGTC CTGTTGCCTG

951 AGCCCTGGGG GGCGGGCGGA GCTAGAGGGG CGCGACAAGG ACCAGATGCT

1001 GCAGGAGAAA GACAAGCAGA TCGAGGCGCT GACGCGCATG CTCCGGCAGA

1051 AGCAGCAGCT GGTGGAGCGG CTCAAGCTGC AGCTGGAGCA GGAGAAGCGA

1101 GCCCAGCAGC CCGCCCCCGC CCCCGCCCCC CTCGGCACCC CCGTGAAGCA

1151 GGAGAACAGC TTCTCCAGCT GCCAGCTGAG CCAGCAGCCC CTGGGCCCCG

1201 CTCACCCATT CAACCCCAGC CTGGCGGCCC CAGCCACCAA CCACATAGAC

1251 CCTTGTGCTG TGGCCCCAGG GCCCCCGTCC GTGGTGGTGA AGCAGGAAGC

1301 CTTGCAGCCT GAGCCCGAGC CGGTCCCCGC CCCCCAGTTG CTTCTGGGGC

1351 CTCAGGGCCC CGGCCTCATC AAGGGGGTTG CACCTCCCAC CCTCATCACC

1401 GACTCCACAG GGACCCACCT TGTCCTCACC GTGACCAATA AGAATGCAGA

1451 CAGCCCTGGC CTGTCCAGTG GGAGCCCCCA GCAGCCCTCG TCCCAGCCTG

1501 GCTCTCCAGC GCCTGCCCCC TCTGCCCAGA TGGACCTGGA GCACCCACTG

1551 CAGCCCCTCT TTGGGACCCC CACTTCTCTG CTGAAGAAGG AACCACCTGG

1601 CTATGAGGAA GCCATGAGCC AGCAGCCCAA ACAGCAGGAA AATGGTTCCT

1651 CAAGCCAGCA GATGGACGAC CTGTTTGACA TTCTCATTCA GAGCGGAGAA

1701 ATTTCAGCAG ATTTCAAGGA GCCGCCATCC CTGCCAGGGA AGGAGAAGCC

1751 ATCCCCGAAG ACAGTCTGTG GGTCCCCCCT GGCAGCACAG CCATCACCTT

1801 CTGCTGAGCT CCCCCAGGCT GCCCCACCTC CTCCAGGCTC ACCCTCCCTC

1851 CCTGGACGCC TGGAGGACTT CCTGGAGAGC AGCACGGGGC TGCCCCTGCT

1901 GACCAGTGGG CATGACGGGC CAGAGCCCCT TTCCCTCATT GACGACCTCC

1951 ATAGCCAGAT GCTGAGCAGC ACTGCCATCC TGGACCACCC CCCGTCACCC

2001 ATGGACACCT CGGAATTGCA CTTTGTTCCT GAGCCCAGCA GCACCATGGG

2051 CCTGGACCTG GCTGATGGCC ACCTGGACAG CATGGACTGG CTGGAGCTGT

2101 CGTCAGGTGG TCCCGTGCTG AGCCTAGCCC CCCTCAGCAC CACAGCCCCC

2151 AGCCTCTTCT CCACAGACTT CCTCGATGGC CATGATTTGC AGCTGCACTG

2201 GGATTCCTGC TTGTAGCTCT CTGGCTCAAG ACGGGGTGGG GAAGGGGCTG

2251 GGAGCCAGGG TACTCCAATG CGTGGCTCTC CTGCGTGATT CGGCCTCTCC

2301 ACATGGTTGT GAGTCTTGAC AATCACAGCC CCTGCTTTTT CCCTTCCCTG

2351 GGAGGCTAGA ACAGAGAAGC CCTTACTCCT GGTTCAGTGC CACGCAGGGC

2401 AGAGGAGAGC AGCTGTCAAG AAGCAGCCCT GGCTCTCACG CTGGGGTTTT

2451 GGACACACGG TCAGGGTCAG GGCCATTTCA GCTTGACCTC CTTTTTTGAG

2501 GTCAGGGGGC ACTGTCTGTC TGGCTACAAT TTGGCTAAGG TAGGTGAAGC

2551 CTGGCCAGGC GGGAGGCTTC TCTTCTGACC CAGGGCTGAG ACAGGTTAAG

2601 GGGTGAATCT CCTTCCTTTC TCTCCCTGCT TTGCTGTGAA GGGAGAAATT

2651 AGCCTGGGCC TCTACCCCCT ATTCCCTGTG TCTGCCAACC CCAGGATCCC

2701 AGGGCTCCCT GCCATTTTAG TGTCTTGGTG TAGTGTAACC ATTTAGTGGT

2751 TGGTGGCAAC AATTTTATGT ACAGGTGTAT ATACCTCTAT ATTATATATC

2801 GACATACATA TATATTTTTG GGGGGGGGCG GACAGGAGAT GGGTGCAACT 2851 CCCTCCCATC CTACTCTCAC AGAAGGGCCT GGATGCAAGG TTACCCTTGA

2901 GCTGTGTGCC ACAGTCTGGT GCCCAGTCTG GCATGCAGCT ACCCAGGCCC

2951 ACCCATCACG TGTGATTGAC ATGTAGGTAC CCTGCCACGG CCTATGCCCC

3001 ACCTGCCCTG CTTCCTGGCT CCTTATCAGT GCCATGAGGG CAGAGGTGCT

3051 ACCTGGCCTT CCTGCCAGGA GCTCTCCACC CACTCACATT CCGTCCCCGC

3101 CGCCTCACTG CAGCCAGCGT GGCCCTAGGA CAGGAGGAGC TTCGGGCCCA

3151 GCTTCACCCT GCGGTGGGGC TGAGGGGTGG CCATCTCCTG CCCTGGGGCC

3201 ACTGGCTTCA CATTCTGGGC TGACTCATAG GGGAGTAGGG GTGGAGTCAC

3251 CAAAACCAGT GCTGGGACAA AGATGGGGAA GGTGTGTGAA CTTTTTAAAA

3301 TAAACACAAA AACACAGGAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

3351 AAG

BLAST Results

Entry HS1042K10 from database EMBL:

Human DNA sequence from clone 1042K10 on chromosome 22ql3.1-13.2. Contains the ADSL gene for Adenylosuccmate lyase (EC 4.3.2.2, Adenylosuccinase, ASL) and 4 novel genes (one with probable rabGAP domains and Src homology domain 3) . Contains ESTs, STSs, GSSs and a putative CpG island.

Score = 7997, P = O.Oe+00, identities = 1617/1645 7 exons

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 183 bp to 2213 bp; peptide length: 677 Category: similarity to unknown protein Classification: unclassified

1 MDSSYAKILQ QQQLFLQLQI LNQQQQQHHN YQAILPAPPK SAGEALGSSG 51 TPPVRSLΞTT NSSSSSGAPG PCGLARQNST SLTGKPGALP ANLDDMKVAE 101 LKQELKLRSL PVSGTKTELI ERLRAYQDQI SPVPGAPKAP AATSILHKAG 151 EVVVAFPAAR LSTGPALVAA GLAPAEVVVA TVASSGVVKF GSTGSTPPVS 201 PTPSERSLLS TGDENSTPGD TFGEMVTSPL TQLTLQASPL QILVKEEGPR 251 AGSCCLSPGG RAELEGRDKD QMLQEKDKQI EALTRMLRQK QQLVERLKLQ 301 LEQEKRAQQP APAPAPLGTP VKQENSFSSC QLSQQPLGPA HPFNPSLAAP 351 ATNHIDPCAV APGPPSVVVK QEALQPEPEP VPAPQLLLGP QGPGLIKGVA 401 PPTLITDSTG THLVLTVTNK NADSPGLSSG SPQQPSSQPG SPAPAPSAQM 451 DLEHPLQPLF GTPTΞLLKKE PPGYEEAMSQ QPKQQENGSS SQQMDDLFDI 501 LIQSGEISAD FKEPPSLPGK EKPSPKTVCG SPLAAQPΞPS AELPQAAPPP 551 PGSPSLPGRL EDFLESSTGL PLLTSGHDGP EPLSLIDDLH SQMLSSTAIL 601 DHPPSPMDTS ELHFVPEPSS TMGLDLADGH LDSMDWLELS SGGPVLSLAP 651 LSTTAPSLFS TDFLDGHDLQ LHWDSCL

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_22nl3, frame 3

TREMBL:HS1042K10_6 gene: "dJ1042K10.3"; product: "dJ1042K10.3 (novel protein)"; Human DNA sequence from clone 1042K10 on chromosome 22ql3.1-13.2. Contains the ADSL gene for Adenylosuccmate lyase (EC 4.3.2.2, Adenylosuccinase, ASL) and 4 novel genes (one with probable rabGAP domains and Src homology domain 3) . Contains ESTs, STSs, GSSs and a putative CpG island., N = 1, Score = 1285, P = 4.9e-131

TREMBL :CEUK06A9_3 gene: "K06A9.1a"; Caenorhabditis elegans cosmid K06A9., N = 2, Score = 149, P = 1.3e-09

TREMBLNEW: SSI132828_1 product: "p210 protein"; Spermatozopsis similis mRNA for p210 protein, partial, N = 1, Score = 171, P = 2.8e-09

>TREMBL:HS1042K10_6 gene: "dJ1042κl0.3"; product: "dJ1042K10.3 (novel protein)"; Human DNA sequence from clone 1042K10 on chromosome 22ql3.1-13.2. Contains the ADSL gene for Adenylosuccmate lyase (EC 4.3.2.2, Adenylosuccinase, ASL) and 4 novel genes (one with probable rabGAP domains and Src homology domain 3) . Contains ESTs, STSs, GSSs and a putative CpG island. Length = 243

HSPs:

Score = 1285 (192.8 bits), Expect = 4.9e-131, P = 4.9e-131 Identities = 243/243 (100%), Positives = 243/243 (100%)

Query: 435 PSSQPGΞPAPAPSAQMDLEHPLQPLFGTPTSLLKKEPPGYEEAMSQQPKQQENGSSSQQM 494

PΞSQPGSPAPAPSAQMDLEHPLQPLFGTPTSLLKKEPPGYEEAMSQQPKQQENGSSSQQM Sbjct: 1 PSSQPGΞPAPAPSAQMDLEHPLQPLFGTPTSLLKKEPPGYEEAMΞQQPKQQENGΞSSQQM 60

Query: 495 DDLFDILIQΞGEISADFKEPPSLPGKEKPSPKTVCGSPLAAQPSPSAELPQAAPPPPGΞP 554

DDLFDILIQSGEISADFKEPPSLPGKEKPSPKTVCGSPLAAQPSPSAELPQAAPPPPGSP Sbjct: 61 DDLFDILIQSGEISADFKEPPSLPGKEKPSPKTVCGSPLAAQPSPSAELPQAAPPPPGSP 120

Query: 555 SLPGRLEDFLESSTGLPLLTSGHDGPEPLSLIDDLHSQMLSSTAILDHPPSPMDTSELHF 614

SLPGRLEDFLESSTGLPLLTSGHDGPEPLSLIDDLHΞQMLSSTAILDHPPSPMDTSELHF Sbjct: 121 SLPGRLEDFLESSTGLPLLTSGHDGPEPLSLIDDLHSQMLSSTAILDHPPSPMDTSELHF 180

Query: 615 VPEPSSTMGLDLADGHLDSMDWLELSSGGPVLSLAPLSTTAPSLFSTDFLDGHDLQLHWD 674

VPEPSSTMGLDLADGHLDSMDWLELSSGGPVLSLAPLSTTAPSLFSTDFLDGHDLQLHWD Sbjct: 181 VPEPSSTMGLDLADGHLDSMDWLELSSGGPVLSLAPLSTTAPSLFSTDFLDGHDLQLHWD 240

Query: 675 SCL 677

SCL Sbjct: 241 SCL 243

Pedant information for DKFZphtes3_22nl3, frame 3

Report for DKFZphtes3_22nl3.3

[LENGTH] 677 [MW] 70743.01 [pi] 4.93 [HOMOL] TREMBL :HS1042K10_6 gene: "dJ1042K10.3"; product: "dJ1042K10.3 (novel protein)'

Human DNA sequence from clone 1042K10 on chromosome 22ql3.1-13.2. Contains the ADSL gene for

Adenylosuccmate lyase (EC 4.3.2.2, Adenylosuccinase, ASL) and 4 novel genes (one with probable rabGAP domains and Src homology domain 3) . Contains ESTs, STSs, GSSs and a putative

CpG island, le-111

[KW] TRANSMEMBRANE 1

[KW] LOW_COMPLEXITY 21.57 %

[KW] COILED COIL 4.58 %

SEQ MDSSYAKILQQQQLFLQLQILNQQQQQHHNYQAILPAPPKSAGEALGSSGTPPVRSLSTT SEG xxxxxxxxxxxxxxxxxxx xxxxx PRD ccchhhhhhhhhhhhhhhhhhhhhhhhcceeeeeecccccceeeecccccccceeecccc COILS

MEM

SEQ NSSSSSGAPGPCGLARQNSTSLTGKPGALPANLDDMKVAELKQELKLRSLPVSGTKTELI

SEG xxxxx

PRD cccccccccccceeecccccccccccccccccccchhhhhhhhhhhhhhcccccchhhhh

COILS

MEM

SEQ ERLRAYQDQISPVPGAPKAPAATSILHKAGEVVVAFPAARLSTGPALVAAGLAPAEVVVA

SEG xxxxxxxxxxxxxxxxx

PRD hhhhhhhhhcccccccccccceeeeeeeccceeeeccccccccccccccccccceeeeee

COILS

MEM . MMMMMMMMMMMMMMMMMMMMMM

SEQ TVASSGVVKFGSTGSTPPVSPTPSERSLLSTGDENSTPGDTFGEMVTSPLTQLTLQASPL

SEG xxxxxxx .. xxxxxxxxxxxxxx

PRD eeecccccccccccccccccccccceeeeccccccccccccccceeecccceeeecccce

COILS

MEM M.

SEQ QILVKEEGPRAGSCCLSPGGRAELEGRDKDQMLQEKDKQIEALTRMLRQKQQLVERLKLQ

SEG

PRD eeeeeccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCCC

MEM

SEQ LEQEKRAQQPAPAPAPLGTPVKQENSFSSCQLSQQPLGPAHPFNPSLAAPATNHIDPCAV SEG xxxxxxxxxx PRD hhhhhhhhhcccccccccccccccccceeeeecccccccccccccceeeccccccccccc COILS ccccccc MEM

SEQ APGPPSVVVKQEALQPEPEPVPAPQLLLGPQGPGLIKGVAPPTLITDSTGTHLVLTVTNK

SEG xxxxxxxxxxxx

PRD cccccceeeeeccccccccccccceeeccccccceeeeecccccccccccceeeeeeecc

COILS

MEM

SEQ NADSPGLSSGSPQQPSSQPGSPAPAPSAQMDLEHPLQPLFGTPTSLLKKEPPGYEEAMSQ

SEG ... xxxxxxxxxxxxxxxxxxxxxxxxxx

PRD ccccccccccccccccccccccccccchhhhhhhhhcccccccccccccccccccccccc

COILS

MEM

SEQ QPKQQENGSSSQQMDDLFDILIQSGEISADFKEPPSLPGKEKPSPKTVCGSPLAAQPSPS

SEG xxxxxxxxxxx

PRD ccccccccccccchhhhhhhhhcccccccccccccccccccccccccccccccccccccc

COILS

MEM

SEQ AELPQAAPPPPGSPSLPGRLEDFLESSTGLPLLTSGHDGPEPLSLIDDLHSQMLSSTAIL

SEG xxxxxxxxxxxxxxxxxx

PRD ccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhcccceee

COILS

MEM

SEQ DHPPSPMDTSELHFVPEPSSTMGLDLADGHLDSMDWLELSSGGPVLSLAPLSTTAPSLFS

SEG

PRD ccccccccccccccccccccccccccccccccccceeeeccccceeeeeecccccccccc

COILS

MEM

SEQ TDFLDGHDLQLHWDSCL SEG PRD cccccccceeecccccc COILS

MEM

(No Prosite data available for DKFZphtes3_22nl3.3) (No Pfam data available for DKFZphtes3_22nl3.3)

DKFZphtes3 23111

group: intracellular transport and trafficking

DKFZphtes3_23111 encodes a novel 186 amino acid protein nearly identical to mouse ADP- ribosylation-like factor homolog 6 (Arl6) .

Protein secretion through the endoplasmic reticulum and the Golgi vesicular trafficking system is initiated by the binding of ADP-ribosylation factors

(ARFs) to donor membranes, leading to recruitment of cocatomer, bud formation, and eventual vesicle release. ARFs are approximately 20-kDa GTPases that are active with bound GTP and inactive with GDP bound. The novel protein contains an ATP/GTP-bmding site motif A (P-loop) and seems to be a novel ARF. It seems to have an important role in vesicular transport and vesicular trafficking.

The new protein can find application in modulating vesicle transport and trafficking in cells. nearly identical to mouse Arl6, ADP-ribosylation-like factor homolog start at Bp 15 matches kozak consensus ANNatgG

Sequenced by LMU

Locus : unknown

Insert length: 717 bp

Poly A stretch at pos. 689, no polyadenylation signal found

1 ATTTGAATCA CATTATGGGA TTGCTAGACA GACTTTCAGT CTTGCTTGGC 51 CTGAAGAAGA AGGAGGTTCA TGTTTTGTGC CTTGGGCTAG ATAATAGTGG 101 CAAAACGACG ATCATTAACA AACTTAAACC TTCAAATGCT CAATCTCAAA 151 ATATCCTTCC AACAATAGGA TTCAGCATAG AGAAATTCAA ATCATCCAGT 201 TTGTCATTTA CAGTGTTTGA CATGTCAGGT CAAGGAAGAT ACAGAAATCT 251 CTGGGAACAC TATTATAAAG AAGGCCAAGC TATTATTTTT GTCATTGATA 301 GTAGTGATAG ATTAAGAATG GTTGTGGCCA AAGAAGAACT CGATACTCTT 351 CTGAATCATC CAGATATTAA ACACCGTCGA ATTCCAATCT TATTCTTTGC 401 AAATAAAATG GATCTTAGAG ATGCAGTGAC ATCTGTAAAA GTGTCTCAGT 451 TGCTGTGTTT AGAGAACATC AAAGATAAAC CCTGGCATAT TTGTGCTAGT 501 GATGCCATAA AAGGAGAAGG CTTGCAAGAA GGTGTAGACT GGCTTCAAGA 551 TCAGATCCAG ACTGTGAAGA CATGAAAAGA TAATAGTTGG AAACCTCAGC 601 AATTTTCAAT TCAAGGAATC TATCTAAGAC AAATAGAATA CATTTTGTAA 651 AAGATGTTTA TGCATCAAAA AATATAATTT TCTGCTTGCA AAAAAAAAAA 701 AAAAAAAAAA AAAAAAG

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 15 bp to 572 bp; peptide length: 186 Category: strong similarity to known protein Classification: Intacellular transport and traffic Prosite motifs: ATP GTP A (24-32)

1 MGLLDRLSVL LGLKKKEVHV LCLGLDNSGK TTIINKLKPS NAQSQNILPT 51 IGFSIEKFKS SSLSFTVFDM SGQGRYRNLW EHYYKEGQAI IFVIDSSDRL 101 RMVVAKEELD TLLNHPDIKH RRIPILFFAN KMDLRDAVTS VKVSQLLCLE 151 NIKDKPWHIC ASDAIKGEGL QEGVDWLQDQ IQTVKT

BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_23111, frame 3

TREMBL:AF031903_1 gene: "Arl6"; product: "ADP-ribosylation-like factor homolog ARL6"; Mus musculus ADP-ribosylation-like factor homolog ARL6 (Arl6) mRNA, complete eds., N = 1, Score = 923, P = l.le-92

TREMBL :CEC38D4_5 gene: "C38D4.8"; Caenorhabditis elegans cosmid C38D4, N = 1, Score = 418, P = 3.6e-39

PIR:Ξ66337 ADP-ribosylation factor 1 - Chlamydomonas reinhardtn, N = 1, Score = 373, P = 2. le-34

SWISSPROT :ARF1_CHLRE ADP-RIBOSYLATION FACTOR 1., N = 1, Score = 372, P = 2.7e-34

>TREMBL:AF031903_1 gene: "Arl6"; product: "ADP-ribosylation-like factor homolog ARL6"; Mus musculus ADP-ribosylation-like factor homolog ARL6 (Arl6) mRNA, complete eds. Length = 186

HSPs:

Score = 923 (138.5 bits). Expect = l.le-92, P = l.le-92 Identities = 178/186 (95%), Positives = 184/186 (98%)

Query: 1 MGLLDRLSVLLGLKKKEVHVLCLGLDNSGKTTIINKLKPSNAQSQNILPTIGFSIEKFKS 60

MGLLDRLS LLGLKKKEVHVLCLGLDNSGKTTIINKLKPSNAQSQ+I+PTIGFSIEKFKS Sbjct: 1 MGLLDRLSGLLGLKKKEVHVLCLGLDNSGKTTIINKLKPSNAQSQDIVPTIGFSIEKFKS 60

Query: 61 SSLSFTVFDMSGQGRYRNLWEHYYKEGQAIIFVIDSSDRLRMVVAKEELDTLLNHPDIKH 120

SSLSFTVFDMSGQGRYRNLWEHYYK+GQAIIFVIDSSD+LRMVVAKEELDTLLNHPDIKH Sbjct: 61 SSLSFTVFDMSGQGRYRNLWEHYYKDGQAIIFVIDSSDKLRMVVAKEELDTLLNHPDIKH 120

Query: 121 RRIPILFFANKMDLRDAVTSVKVSQLLCLENIKDKPWHICAΞDAIKGEGLQEGVDWLQDQ 180

RRIPILFFANKMDLRD+VTSVKVΞQLLCLE+IKDKPWHICASDAIKGEGLQEGVDWLQDQ Sbjct: 121 RRIPILFFANKMDLRDSVTSVKVSQLLCLESIKDKPWHICASDAIKGEGLQEGVDWLQDQ 180

Query: 181 IQTVKT 186

IQ VKT Sbjct: 181 IQAVKT 186

Pedant information for DKFZphtes3_23111, frame 3

Report for DKFZphtes3_23111.3

[LENGTH] 186 [MW] 21097.69 [pi] 8.72 [HOMOL] TREMBL :AF031903_1 gene: "Arl6"; product: "ADP-ribosylation-like factor homolog

ARL6"; Mus musculus ADP-ribosylation-like factor homolog ARL6 (Arl6) mRNA, complete eds. 4e-94

[FUNCAT] 30.08 organization of golgi [S. cerevisiae, YDL192w] le-36

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YDL192w] le-36

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL192w] le-36

[FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae,

YDL137w] 2e-36

[FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) S. cerevisiae, YBR164c] 2e-32

[FUNCAT] 30.03 organization of cytoplasm S. cerevisiae, YBR164C] 2e-32

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YMR138w] 4e-19

[FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YMR138w] 4e-19

[FUNCAT] r general function prediction [M. jannaschn MJ1339] 2e-05

[FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YHR005c] 4e-05

[FUNCAT] 03.07 pheromone response, matmg-type determination, sex-specific proteins

[S. cerevisiae, YHR005c] 4e-05

[FUNCAT] 10.05.07 g-proteins [S. cerevisiae, YHR005C] 4e-05

[FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YKR014c] 2e-04

[FUNCAT] 08.19 cellular import [S. cerevisiae, YKR014c] 2e-04

[FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YKR014c]

2e-04

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YFL005w]

4e-04

[BLOCKS] BL01288C

[BLOCKS] BL01020C SARI family proteins

[BLOCKS] BL01019C ADP-ribosylation factors family proteins [BLOCKS] BL01019B ADP-ribosylation factors family proteins

[BLOCKS] BL01019A ADP-ribosylation factors family proteins

[SCOP] dlas3_2 3.29.1.4.12 Transduem (alpha subunit), insertion domai 2e-45

[SCOP] dlmhl 3.29.1.4.2 Racl [Human (Homo sapiens) 2e-46

[SCOP] d5p₂l 3.29.1.4.1 cH-p21 Ras protein [human (Homo sapiens) 5e-37

[SCOP] dlhura_ 3.29.1.4.8 ADP-ribosylation factor 1 (ARFl) [human (Hom 4e-61

[SCOP] dla2kc_ 3.29.1.4.5 Ran Nuclear transport factor-2 (NTF2) [Do 4e-33

[PIRKW] glycoprotein 2e-33

[PIRKW] monomer 3e-31

[PIRKW] P-loop 2e-35

[PIRKW] lipoprotein 2e-33

[PIRKW] GTP binding 2e-35

[SUPFAM] ADP-ribosylation factor 2e-35

[PROSITE] ATP_GTP_A 1

[PFAM] ADP-ribosylation factors (Arf family) (contains ATP/GTP binding P-loop)

[KW] Alpha_Beta

[KW] 3D

[KW] LOW COMPLEXITY 5.91 %

SEQ MGLLDRLSVLLGLKKKEVHVLCLGLDNSGKTTIINKLKPSNAQSQNILPTIGFSIEKFKS

SEG .. xxxxxxxxxxx lhurA CCCCEEEEEETTTTCHHHHHHHHCCCCEEEE—EEETTEEEEEEEE

SEQ SSLSFTVFDMSGQGRYRNLWEHYYKEGQAIIFVIDSSDRLRMVVAKEELDTLLNHPDIKH

SEG lhurA TTEEEEEEETTTTTTTCCCHHHHHHCEEEEEEEEETTTTTHHHHHHHHHHHHHHTTTT—

SEQ RRIPILFFANKMDLRDAVTSVKVSQLLCLENIKDKPWHICASDAIKGEGLQEGVDWLQDQ

SEG lhurA TTTEEEEEEETTTTTTTCCHHHHHHHHCGGGTTTTCEEEEECBTTTTBTHHHHHHHHHHH

SEQ IQTVKT

SEG lhurA HHHHC.

Prosite for DKFZphtes3_23111.3

PS00017 24->32 ATP GTP A PDOC00017

Pfam for DKFZphtes3_23111.3

HMM_NAME ADP-ribosylation factors (Arf family) (contains ATP/GTP binding P-loop)

HMM *GMgWfsIFrkMWGlWNKEMRILMLGLDNAGKTTILYMLKlgE.. IVTTI MG++ ++ ++GL +KE+++L LGLDN+GKTTI+++LK+ ++

Query 1 -MGLLDRLSVLLGLKKKEVHVLCLGLDNSGKTTIINKLKPSNAQSQNIL 48

HMM PTIGFNVETVeYKNIKFNVWDVGGQdsIRPYWRHYYpNTDGIIWVVDSaD PTIGF +E+ + ++F+V+D GQ + R +W HYY + ++II+V+DS+D

Query 49 PTIGFSIEKFKSSSLSFTVFDMSGQGRYRNLWEHYYKEGQAIIFVIDSSD 98

HMM RDRMeEaKqELHaMLNEEEL.. rDAPlLIFANKQDLPgAMSesEIREaLG R RM AK+EL+ +LN+ ++ R+ P+L FANK DL++A+++ +++ +L

Query 99 RLRMVVAKEELDTLLNHPDIKHRRIPILFFANKMDLRDAVTSVKVSQLLC 148

HMM LHelRCnRPWYIQMCCAVtGEGLYEGMDWLSNYInkRkK* L++I+ + PW+I +++A++GEGL+EG DWL ++I+ K

Query 149 LENIK-DKPWHICASDAIKGEGLQEGVDWLQDQIQTVKT 186 DKFZphtes3_23nl9

group: testes derived

DKFZphtes3_23nl9 encodes a novel 387 amino acid protein with similarity to rat protein kinase C-mteracting RBCC protein 1.

The novel protein contains not the RING-B box-coiled coil (RBCC) motif of RBCC protein 1, and thus is not a member of this subgroup of RING finger proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . similarity to rat protein kinase C-interacting RBCC protein 1 start at Bp 209 matches kozak consensus PyNNatgG similarity to of C-termmal part to N-termmus of RBCK1

Sequenced by LMU

Locus : unknown

Insert length: 1579 bp

Poly A stretch at pos. 1535, polyadenylation signal at pos. 1515

1 CGGAGACCCT CGGGCCGTGT CCATTTGTGG GCAAAGCCAG CGGGGCAGGC

51 TTGGCCAGAG TGCACCACTC GGCGCCGTCC CAGGCCCGAC GCTCTGGGCG

101 CGCCCGGAAC CCCAGGTTCG CGGCCCGTGT TTCCGACCGG CGGAGGGGGC

151 TCAGCGGCCC GATCCCACGG AAGCGCGCTC GGAGGGGTGG GACCCGGCCG

201 GACCGGAGAT GGCGCCGCCA GCGGGCGGGG CGGCGGCGGC GGCCTCGGAC

251 TTGGGCTCCG CCGCAGTGCT CTTGGCTGTG CACGCCGCGG TGAGGCCGCT

301 GGGCGCCGGG CCAGACGCCG AGGCACAGCT GCGGAGGCTG CAGCTGAGCG

351 CGGACCCTGA GAGGCCTGGG CGCTTCCGGC TGGAGCTGCT GGGCGCGGGA

401 CCTGGGGCGG TTAATTTGGA GTGGCCCCTG GAGTCAGTTT CCTACACCAT

451 CCGAGGCCCC ACCCAGCACG AGCTACAGCC TCCACCAGGA GGGCCTGGAA

501 CCCTCAGCCT GCACTTCCTC AACCCTCAGG AAGCTCAGCG GTGGGCAGTC

551 CTAGTCCGAG GTGCCACCGT GGAAGGACAG AATGGCAGCA AGAGCAACTC

601 ACCACCAGCC TTGGGCCCAG AAGCATGCCC TGTCTCCCTG CCCAGTCCCC

651 CGGAAGCCTC CACACTCAAG GGCCCTCCAC CTGAGGCAGA TCTTCCTAGG

701 AGCCCTGGAA ACTTGACGGA GAGAGAAGAG CTGGCAGGGA GCCTGGCCCG

751 GGCTATTGCA GGTGGAGACG AGAAGGGGGC AGCCCAAGTG GCAGCCGTCC

801 TGGCCCAGCA TCGTGTGGCC CTGAGTGTTC AGCTTCAGGA GGCCTGCTTC

851 CCACCTGGCC CCATCAGGCT GCAGGTCACA CTTGAAGACG CTGCCTCTGC

901 CGCATCCGCC GCGTCCTCTG CACACGTTGC CCTGCAGGTC CACCCCCACT

951 GCACTGTTGC AGCTCTCCAG GAGCAGGTGT TCTCAGAGCT CGGTTTCCCG

1001 CCAGCCGTGC AACGCTGGGT CATCGGACGG TGCCTGTGTG TGCCTGAGCG

1051 CAGCCTTGCC TCTTACGGGG TTCGGCAGGA TGGGGACCCT GCTTTCCTCT

1101 ACTTGCTGTC AGCTCCTCGA GAAGCCCCAG CCACAGGACC TAGCCCTCAG

1151 CACCCCCAGA AGATGGACGG GGAACTTGGA CGCTTGTTTC CCCCATCATT

1201 GGGGCTACCC CCAGGCCCCC AGCCAGCTGC CTCCAGCCTG CCCAGTCCAC

1251 TCCAGCCCAG CTGGTCCTGT CCTTCCTGCA CCTTCATCAA TGCCCCAGAC

1301 CGCCCTGGCT GTGAGATGTG TAGCACCCAG AGGCCCTGCA CTTGGGACCC

1351 CCTTGCTGCA GCTTCCACCT AGCAGCCACC AGAGGTTACA AGGGGAGAGT

1401 GGCCCTTCCC TCACAAGTCC GACATCTCCA GGCCCCCACT GAACTCCGGG

1451 GACCTCTACT GACTGCTTGC TGGGACAGTC ACCAGGGTTG GGGGGAAGGG

1501 CCACAAAATG AAACCATTAA AGACCCTTAA GAGCCAAAAA AAAAAAAAAA

1551 AAAAAAAAAA AAAAAAAAAA AAAAAAAAG

BLAST Results

No BLAST result

Medlme entries

No Medl e entry

Peptide information for frame 2 ORF from 209 bp to 1369 bp; peptide length: 387 Category: similarity to known protein Classification: Cell signaling/communication

1 MAPPAGGAAA AASDLGSAAV LLAVHAAVRP LGAGPDAEAQ LRRLQLSADP

51 ERPGRFRLEL LGAGPGAVNL EWPLESVSYT IRGPTQHELQ PPPGGPGTLS

101 LHFLNPQEAQ RWAVLVRGAT VEGQNGSKSN SPPALGPEAC PVΞLPSPPEA

151 STLKGPPPEA DLPRSPGNLT EREELAGSLA RAIAGGDEKG AAQVAAVLAQ

201 HRVALSVQLQ EACFPPGPIR LQVTLEDAAS AASAASSAHV ALQVHPHCTV

251 AALQEQVFSE LGFPPAVQRW VIGRCLCVPE RSLASYGVRQ DGDPAFLYLL

301 ΞAPREAPATG PSPQHPQKMD GELGRLFPPS LGLPPGPQPA ASSLPSPLQP

351 SWSCPSCTFI NAPDRPGCEM CSTQRPCTWD PLAAAST

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_23nl9, frame 2

PIR:JC5983 protein kinase C-interacting RBCC protein 1 - rat, N = 1, Score = 353, P = 2.8e-32

TREMBL :AB011369_1 product: "RBCK2"; Rattus norvegicus mRNA for RBCK2, complete eds., N = 1, Score = 353, P = 2.8e-32

TREMBL :U67322_1 gene: "XAP4"; product: "HBV associated factor"; Human HBV associated factor (XAP4) mRNA, complete eds., N = 1, Score = 286, P = 8.5e-25

TREMBLNEW:AF124663_1 product: "UbcM4 interacting protein 28"; Mus musculus UbcM4 interacting protein 28 mRNA, complete eds., N = 1, Score = 367, P = 9.3e-34

>TREMBLNEW: AF124663_1 product: "UbcM4 interacting protein 28"; Mus musculus UbcM4 interacting protein 28 mRNA, complete eds. Length = 498

HSPs:

Score = 367 (55.1 bits), Expect = 9.3e-34, P = 9.3e-34 Identities = 95/212 (44%), Positives = 129/212 (60%)

Query: 175 LAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASA 234

+A SLARA+AGGDE+ A + A LA+ RV L VQ++ P IRL V++EDA Sbjct: 1 MALSLARAVAGGDEQAAIKYATWLAEQRVPLRVQVKPEVSPTQDIRLCVSVEDAYM 56

Query: 235 ASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDP 294

+ + L V P TVA+L++ VF + GFPP++Q+WV+G+ L + +L S+G+R++GD Sbjct: 57 -HTVTIWLTVRPDMTVASLKDMVFLDYGFPPSLQQWVVGQRLARDQETLHSHGIRRNGDG 115

Query: 295 AFLYLLSAPREAPATGPSPQHPQK MDGELG—RLFPPSLG-LPPG-PQPAASSLP 345

A+LYLLSA T +PQ Q+ M +LG L S G L P P+P + P Sbjct: 116 AYLYLLSARN TSLNPQELQRQRQLRMLEDLGFKDLTLQSRGPLEPVLPKPRTNQEP 171

Query: 346 SPLQP—ΞWSCPSCTFINAPDRPGCEMCSTQRPCTW 379

+P P W CP CTFIN P RPGCEMC RP T+ Sbjct: 172 GQPDAAPESPPVGWQCPGCTFINKPTRPGCEMCCRARPETY 212

Pedant information for DKFZphtes3_23nl9, frame 2

Report for DKFZphtes3_23nl9.2

[LENGTH] 387

[MW] 39949.29

[pi] 5.53

[HOMOL] TREMBLNEW:AF124663_1 product: "UbcM4 interacting protein 28"; Mus musculus

UbcM4 interacting protein 28 mRNA, complete eds. le-22

[BLOCKS] BL00578B

[KW] Alpha_Beta

[KW] LOW_COMPLEXITY 17.57 %

SEQ MAPPAGGAAAAASDLGΞAAVLLAVHAAVRPLGAGPDAEAQLRRLQLSADPERPGRFRLEL

SEG . xxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD cccccchhhhhhhhhhhhhhhhhhhhhccccccccchhhhhhhhhhhccccccccceeee SEQ LGAGPGAVNLEWPLESVSYTIRGPTQHELQPPPGGPGTLSLHFLNPQEAQRWAVLVRGAT

SEG

PRD ccccccceeecccceeeeeeccccccccccccccccceeeeeecccchhhhhheeeecce

SEQ VEGQNGSKSNSPPALGPEACPVSLPSPPEASTLKGPPPEADLPRSPGNLTEREELAGSLA

SEG

PRD eecccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhh

SEQ RAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASAASSAHV

SEG xxxxxxxxxxx ..

PRD hhhhcccchhhhhhhhhhhhhhhhhhccccccccccccceeeccchhhhhhhhhhhhhee

SEQ ALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDPAFLYLL

SEG

PRD eeeccccchhhhhhhhhhhhccccccchhhhhhhhhhhhccccccccccccccceeeeec

SEQ SAPREAPATGPSPQHPQKMDGELGRLFPPSLGLPPGPQPAASSLPSPLQPSWSCPSCTFI

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxx...

PRD cccccccccchhhhhhhhhhhhccccccccccccccccccccccccccccccccccceee

SEQ NAPDRPGCEMCSTQRPCTWDPLAAAST

SEG

PRD ccccccccccccccccccccceeeccc

(No Prosite data available for DKFZphtes3_23nl9.2) (No Pfam data available for DKFZphtes3_23nl9.2)

similarity to rat protein kinase C-mteracting RBCC protein 1 start at Bp 209 matches kozak consensus PyNNatgG similarity to of C-termmal part to N-terminus of RBCK1

Sequenced by LMU

Locus: unknown

Insert length: 1579 bp

Poly A stretch at pos. 1535, polyadenylation signal at pos. 1515

1 CGGAGACCCT CGGGCCGTGT CCATTTGTGG GCAAAGCCAG CGGGGCAGGC

51 TTGGCCAGAG TGCACCACTC GGCGCCGTCC CAGGCCCGAC GCTCTGGGCG

101 CGCCCGGAAC CCCAGGTTCG CGGCCCGTGT TTCCGACCGG CGGAGGGGGC

151 TCAGCGGCCC GATCCCACGG AAGCGCGCTC GGAGGGGTGG GACCCGGCCG

201 GACCGGAGAT GGCGCCGCCA GCGGGCGGGG CGGCGGCGGC GGCCTCGGAC

251 TTGGGCTCCG CCGCAGTGCT CTTGGCTGTG CACGCCGCGG TGAGGCCGCT

301 GGGCGCCGGG CCAGACGCCG AGGCACAGCT GCGGAGGCTG CAGCTGAGCG

351 CGGACCCTGA GAGGCCTGGG CGCTTCCGGC TGGAGCTGCT GGGCGCGGGA

401 CCTGGGGCGG TTAATTTGGA GTGGCCCCTG GAGTCAGTTT CCTACACCAT

451 CCGAGGCCCC ACCCAGCACG AGCTACAGCC TCCACCAGGA GGGCCTGGAA

501 CCCTCAGCCT GCACTTCCTC AACCCTCAGG AAGCTCAGCG GTGGGCAGTC

551 CTAGTCCGAG GTGCCACCGT GGAAGGACAG AATGGCAGCA AGAGCAACTC

601 ACCACCAGCC TTGGGCCCAG AAGCATGCCC TGTCTCCCTG CCCAGTCCCC

651 CGGAAGCCTC CACACTCAAG GGCCCTCCAC CTGAGGCAGA TCTTCCTAGG

701 AGCCCTGGAA ACTTGACGGA GAGAGAAGAG CTGGCAGGGA GCCTGGCCCG

751 GGCTATTGCA GGTGGAGACG AGAAGGGGGC AGCCCAAGTG GCAGCCGTCC

801 TGGCCCAGCA TCGTGTGGCC CTGAGTGTTC AGCTTCAGGA GGCCTGCTTC

851 CCACCTGGCC CCATCAGGCT GCAGGTCACA CTTGAAGACG CTGCCTCTGC

901 CGCATCCGCC GCGTCCTCTG CACACGTTGC CCTGCAGGTC CACCCCCACT

951 GCACTGTTGC AGCTCTCCAG GAGCAGGTGT TCTCAGAGCT CGGTTTCCCG

1001 CCAGCCGTGC AACGCTGGGT CATCGGACGG TGCCTGTGTG TGCCTGAGCG

1051 CAGCCTTGCC TCTTACGGGG TTCGGCAGGA TGGGGACCCT GCTTTCCTCT

1101 ACTTGCTGTC AGCTCCTCGA GAAGCCCCAG CCACAGGACC TAGCCCTCAG

1151 CACCCCCAGA AGATGGACGG GGAACTTGGA CGCTTGTTTC CCCCATCATT

1201 GGGGCTACCC CCAGGCCCCC AGCCAGCTGC CTCCAGCCTG CCCAGTCCAC

1251 TCCAGCCCAG CTGGTCCTGT CCTTCCTGCA CCTTCATCAA TGCCCCAGAC

1301 CGCCCTGGCT GTGAGATGTG TAGCACCCAG AGGCCCTGCA CTTGGGACCC

1351 CCTTGCTGCA GCTTCCACCT AGCAGCCACC AGAGGTTACA AGGGGAGAGT

1401 GGCCCTTCCC TCACAAGTCC GACATCTCCA GGCCCCCACT GAACTCCGGG

1451 GACCTCTACT GACTGCTTGC TGGGACAGTC ACCAGGGTTG GGGGGAAGGG

1501 CCACAAAATG AAACCATTAA AGACCCTTAA GAGCCAAAAA AAAAAAAAAA

1551 AAAAAAAAAA AAAAAAAAAA AAAAAAAAG BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 209 bp to 1369 bp; peptide length: 387 Category: similarity to known protein Classification: Cell signaling/communication

1 MAPPAGGAAA AASDLGSAAV LLAVHAAVRP LGAGPDAEAQ LRRLQLSADP

51 ERPGRFRLEL LGAGPGAVNL EWPLESVSYT IRGPTQHELQ PPPGGPGTLS

101 LHFLNPQEAQ RWAVLVRGAT VEGQNGSKSN SPPALGPEAC PVSLPSPPEA

151 STLKGPPPEA DLPRSPGNLT EREELAGSLA RAIAGGDEKG AAQVAAVLAQ

201 HRVALSVQLQ EACFPPGPIR LQVTLEDAAS AASAASSAHV ALQVHPHCTV

251 AALQEQVFSE LGFPPAVQRW VIGRCLCVPE RSLASYGVRQ DGDPAFLYLL

301 SAPREAPATG PSPQHPQKMD GELGRLFPPS LGLPPGPQPA ASSLPSPLQP

351 SWSCPSCTFI NAPDRPGCEM CSTQRPCTWD PLAAAST

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_23nl9, frame 2

PIR:JC5983 protein kinase C-interactmg RBCC protein 1 - rat, N = 1, Score = 353, P = 2.8e-32

TREMBL :AB011369_1 product: "RBCK2"; Rattus norvegicus mRNA for RBCK2, complete eds., N = 1, Score = 353, P = 2.8e-32

TREMBL :U67322_1 gene: "XAP4"; product: "HBV associated factor"; Human HBV associated factor (XAP4) mRNA, complete eds., N = 1, Score = 286, P = 8.5e-25

TREMBLNEW:AF124663_1 product: "UbcM4 interacting protein 28"; Mus musculus UbcM4 interacting protein 28 mRNA, complete eds., N = 1, Score = 367, P = 9.3e-34

>TREMBLNEW:AF124663_1 product: "UbcM4 interacting protein 28"; Mus musculus UbcM4 interacting protein 28 mRNA, complete eds. Length = 498

HSPs:

Score = 367 (55.1 bits), Expect = 9.3e-34, P = 9.3e-34 Identities = 95/212 (44%), Positives = 129/212 (60%)

Query: 175 LAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASA 234

+A SLARA+AGGDE+ A + A LA+ RV L VQ++ P IRL V++EDA Sbjct: 1 MALSLARAVAGGDEQAAIKYATWLAEQRVPLRVQVKPEVSPTQDIRLCVSVEDAYM 56

Query: 235 ASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDP 294

+ + L V P TVA+L++ VF + GFPP++Q+WV+G+ L + +L S+G+R++GD Sbjct: 57 -HTVTIWLTVRPDMTVASLKDMVFLDYGFPPSLQQWVVGQRLARDQETLHSHGIRRNGDG 115

Query: 295 AFLYLLSAPREAPATGPSPQHPQK MDGELG—RLFPPSLG-LPPG-PQPAASSLP 345

A+LYLLSA T +PQ Q+ M +LG L S G L P P+P + P Sbjct: 116 AYLYLLSARN TSLNPQELQRQRQLRMLEDLGFKDLTLQSRGPLEPVLPKPRTNQEP 171

Query: 346 SPLQP—SWSCPSCTFINAPDRPGCEMCSTQRPCTW 379

+P P W CP CTFIN P RPGCEMC RP T+ Sbjct: 172 GQPDAAPESPPVGWQCPGCTFINKPTRPGCEMCCRARPETY 212

Pedant information for DKFZphtes3_23nl9, frame 2 Report for DKFZphtes3_23nl9.2

[LENGTH] 387

[MW] 39949.29

[pi] 5.53

[HOMOL] TREMBLNEW:AF124663_1 product: "UbcM4 interacting protein 28"; Mus musculus

UbcM4 interacting protein 28 mRNA, complete eds. le-22

[BLOCKS] BL00578B

[KW] Alpha_Beta

[KW] LOW_COMPLEXITY 17.57 %

SEQ MAPPAGGAAAAAΞDLGSAAVLLAVHAAVRPLGAGPDAEAQLRRLQLSADPERPGRFRLEL SEG .xxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD cccccchhhhhhhhhhhhhhhhhhhhhccccccccchhhhhhhhhhhccccccccceeee

SEQ LGAGPGAVNLEWPLESVSYTIRGPTQHELQPPPGGPGTLSLHFLNPQEAQRWAVLVRGAT SEG

PRD ccccccceeecccceeeeeeccccccccccccccccceeeeeecccchhhhhheeeecce

SEQ VEGQNGSKSNSPPALGPEACPVSLPSPPEASTLKGPPPEADLPRSPGNLTEREELAGSLA SEG

PRD eecccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhh

SEQ RAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASAASSAHV

SEG xxxxxxxxxxx ..

PRD hhhhcccchhhhhhhhhhhhhhhhhhccccccccccccceeeccchhhhhhhhhhhhhee

SEQ ALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDPAFLYLL SEG

PRD eeeccccchhhhhhhhhhhhccccccchhhhhhhhhhhhccccccccccccccceeeeec

SEQ SAPREAPATGPSPQHPQKMDGELGRLFPPSLGLPPGPQPAASSLPSPLQPSWSCPSCTFI

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxx...

PRD cccccccccchhhhhhhhhhhhccccccccccccccccccccccccccccccccccceee

SEQ NAPDRPGCEMCSTQRPCTWDPLAAAST SEG

PRD ccccccccccccccccccccceeeccc

(No Prosite data available for DKFZphtes3_23nl9.2) (No Pfam data available for DKFZphtes3_23nl9.2)

DKFZphtes3_26g22

group: intracellular transport/trafficking

DKFZphtes3 26g22 encodes a novel 898 amino acid protein with similarity to kmesms.

The novel protein contains a ATP/GTP-bindmg site motif A (P-loop) and a kinesin motor domain signature. Kinesm is a microtubule-associated force-producing protein that play a role in organelle transport. It is an oligomeric complex composed of two heavy chains and two light chains. The kinesm motor activity is directed toward the microtubule ' s plus end. The heavy chain contains a large globular N-terminal domain which is responsible for the motor activity of kinesin, which is known to hydrolyze ATP and to bind and move on microtubules. Several proteins involved in chromosome segregation and cell divsion contain this motor domain, such as drosophila claret segregational protein (ncd) , Drosophila kinesin-like protein (nod) , human CENP-E and human mitotic kines -like proteιn-1 (MKLP-1). The novel protein is a new kinesin like proptem.

The new protein can find application in modulating chromosome transport in mitosis and meiosis and modulation of cell division. strong similarity to kinesins

Sequenced by EMBL

Locus : unknown

Insert length: 3032 bp

No poly A stretch found, no polyadenylation signal found

1 CTGAAGCGCT GGGAGGCGGA CATTAAAGTG AAGTGGTTGC GGTAACCTGG

51 CCTGGGCCTG AAGTGAGTGA GAGGCACATG AAGAGAAGTA TTCAAGTATT

101 TATACAGATA GGAATCAAGA TAATCAACAA TGTCTGTCAC TGAGGAAGAC

151 CTGTGCCACC ATATGAAAGT AGTAGTTCGT GTACGTCCGG AAAACACTAA

201 AGAAAAAGCA GCTGGATTTC ATAAAGTGGT TCATGTTGTG GATAAACATA

251 TCCTAGTTTT TGATCCCAAA CAAGAAGAAG TCAGTTTTTT CCATGGAAAG

301 AAAACTACAA ATCAAAATGT TATAAAGAAA CAAAATAAGG ATCTTAAATT

351 TGTATTTGAT GCTGTTTTTG ATGAAACGTC AACTCAGTCA GAAGTTTTTG

401 AACACACTAC TAAGCCAATT CTTCGTAGTT TTTTGAATGG ATATAATTGC

451 ACAGTACTTG CCTATGGTGC CACTGGTGCT GGGAAGACCC ACACTATGCT

501 AGGATCAGCT GATGAACCTG GAGTGATGTA TCTAACAATG TTACACCTTT

551 ACAAATGCAT GGATGAGATT AAAGAAGAGA AAATATGTAG TACTGCAGTT

601 TCATATCTGG AGGTATATAA TGAACAGATT CGTGATCTCT TAGTAAATTC

651 AGGGCCACTT GCTGTCCGGG AAGATACCCA AAAAGGGGTG GTCGTTCATG

701 GACTTACTTT ACACCAGCCC AAATCCTCAG AAGAAATTTT ACATTTATTG

751 GATAATGGAA ACAAAAACAG GACACAACAT CCCACTGATA TGAATGCCAC

801 ATCTTCTCGT TCTCATGCTG TTTTCCAAAT TTACTTGCGA CAACAAGACA

851 AAACAGCAAG TATCAATCAA AATGTCCGTA TTGCCAAGAT GTCACTCATT

901 GACCTGGCAG GATCTGAGCG AGCAAGTACT TCCGGTGCTA AGGGGACCCG

951 ATTTGTAGAA GGCACAAATA TTAATAGATC ACTTTTAGCT CTTGGGAATG

1001 TCATCAATGC CTTAGCAGAT TCAAAGAGAA AGAATCAGCA TATCCCTTAC

1051 AGAAATAGTA AGCTTACTCG CTTGTTAAAG GATTCTCTTG GAGGAAACTG

1101 TCAAACTATA ATGATAGCTG CTGTTAGTCC TTCCTCTGTA TTCTACGATG

1151 ACACATATAA CACTCTTAAG TATGCTAACC GGGCAAAGGA CATTAAATCT

1201 TCTTTGAAGA GCAATGTTCT TAATGTCAAT AATCATATAA CTCAATATGT

1251 AAAGATCTGT AATGAGCAGA AGGCAGAGAT TTTATTGTTA AAAGAAAAAC

1301 TAAAAGCCTA TGAAGAACAG AAAGCCTTCA CTAATGAAAA TGACCAAGCA

1351 AAGTTAATGA TTTCAAACCC TCAGGAAAAA GAAATCGAAA GGTTTCAAGA

1401 AATCCTGAAC TGCTTGTTCC AGAATCGAGA AGAAATTAGA CAAGAATATC

1451 TGAAGTTGGA AATGTTACTT AAAGAAAATG AACTTAAATC ATTCTACCAA

1501 CAACAGTGCC ATAAACAAAT AGAAATGATG TGTTCTGAAG ACAAAGTAGA

1551 AAAGGCCACT GGAAAACGAG ATCATAGACT TGCAATGTTG AAAACTCGTC

1601 GCTCCTACCT GGAGAAAAGG AGGGAGGAGG AATTGAAGCA ATTTGATGAG

1651 AATACTAATT GGCTCCATCG TGTCGAAAAA GAAATGGGAC TCTTAAGTCA

1701 AAACGGTCAT ATTCCAAAGG AACTCAAGAA AGATCTTCAT TGTCACCATT

1751 TGCACCTCCA GAACAAAGAT TTGAAAGCAC AAATTAGACA TATGATGGAT

1801 CTAGCTTGTC TTCAGGAACA GCAACACAGG CAGACTGAAG CAGTATTGAA

1851 TGCTTTACTT CCAACCCTAA GAAAACAATA TTGCACATTA AAAGAAGCCG

1901 GCCTGTCAAA TGCTGCTTTT GAATCTGACT TCAAAGAGAT CGAACATTTG

1951 GTAGAGAGGA AAAAAGTGGT AGTTTGGGCT GACCAAACTG CCGAACAACC

2001 AAAGCAAAAC GATCTACCAG GGATTTCTGT TCTTATGACC TTTCCACAAC

2051 TTGGACCAGT TCAGCCTATT CCTTGTTGCT CATCTTCAGG TGGAACTAAT

2101 CTGGTTAAGA TTCCTACAGA AAAAAGAACT CGGAGAAAAC TAATGCCATC

2151 TCCCTTGAAA GGACAGCATA CTCTAAAGTC TCCACCATCT CAAAGTGTGC

2201 AGCTCAATGA TTCTCTTAGC AAAGAACTTC AGCCTATTGT ATATACACCA

2251 GAAGACTGTA GAAAAGCTTT TCAAAATCCG TCTACAGTAA CCTTAATGAA

2301 ACCATCATCA TTTACTACAA GTTTTCAGGC TATCAGCTCA AACATAAACA

2351 GTGATAATTG TCTGAAAATG TTGTGTGAAG TAGCTATCCC TCATAATAGA 2401 AGAAAAGAAT GTGGACAGGA GGACTTGGAC TCTACATTTA CTATATGTGA

2451 AGACATCAAG AGCTCGAAGT GTAAATTACC CGAACAAGAA TCACTACCAA

2501 ATGATAACAA AGACATTTTA CAACGGCTTG ATCCTTCTTC ATTCTCAACT

2551 AAGCATTCTA TGCCTGTACC AAGCATGGTG CCATCCTACA TGGCAATGAC

2601 TACTGCTGCC AAAAGGAAAC GGAAATTAAC AAGTTCTACA TCAAACAGTT

2651 CGTTAACTGC AGACGTAAAT TCTGGATTTG CCAAACGTGT TCGACAAGAT

2701 AATTCAAGTG AGAAGCACTT ACAAGAAAAC AAACCAACAA TGGAACATAA

2751 AAGAAACATC TGTAAAATAA ATCCAAGCAT GGTTAGAAAA TTTGGAAGAA

2801 ATATTTCAAA AGGAAATCTA AGATAAATCA CTTCAAAACC AAGCAAAATG

2851 AAGTTGATCA AATCTGCTTT TCAAAGTTTA TCAATACCCT TTCAAAAATA

2901 TATTTAAAAT CTTTGAAAGA AGACCCATCT TAAAGCTAAG TTTACCCAAG

2951 TACTTTCAGC AAGCAGAAAA ATGAAACTCT TTGTTTTCTT CTTTTGTGTT

3001 CTAAAAAAAT AAAATTTCAA AAGAAAAAAA AA

BLAST Results

No BLAST result

Medlme entries

No Medline entry

Peptide information for frame 1

ORF from 130 bp to 2823 bp; peptide length: 898 Category: strong similarity to known protein Classification: Cell structure/motility Prosite motifs: ATP_GTP_A (113-121) KINESIN MOTOR DOMAIN1 (252-264)

1 MSVTEEDLCH HMKVVVRVRP ENTKEKAAGF HKVVHVVDKH ILVFDPKQEE

51 VSFFHGKKTT NQNVIKKQNK DLKFVFDAVF DETSTQSEVF EHTTKPILRS

101 FLNGYNCTVL AYGATGAGKT HTMLGSADEP GVMYLTMLHL YKCMDEIKEE

151 KICSTAVSYL EVYNEQIRDL LVNSGPLAVR EDTQKGVVVH GLTLHQPKSS

201 EEILHLLDNG NKNRTQHPTD MNATSSRSHA VFQIYLRQQD KTASINQNVR

251 IAKMSLIDLA GSERASTSGA KGTRFVEGTN INRSLLALGN VINALADSKR

301 KNQHIPYRNS KLTRLLKDSL GGNCQTIMIA AVSPSSVFYD DTYNTLKYAN

351 RAKDIKSSLK SNVLNVNNHI TQYVKICNEQ KAEILLLKEK LKAYEEQKAF

401 TNENDQAKLM ISNPQEKEIE RFQEILNCLF QNREEIRQEY LKLEMLLKEN

451 ELKSFYQQQC HKQIEMMCSE DKVEKATGKR DHRLAMLKTR RSYLEKRREE

501 ELKQFDENTN WLHRVEKEMG LLΞQNGHIPK ELKKDLHCHH LHLQNKDLKA

551 QIRHMMDLAC LQEQQHRQTE AVLNALLPTL RKQYCTLKEA GLSNAAFESD

601 FKEIEHLVER KKVVVWADQT AEQPKQNDLP GISVLMTFPQ LGPVQPIPCC

651 SSSGGTNLVK IPTEKRTRRK LMPSPLKGQH TLKSPPSQSV QLNDSLSKEL

701 QPIVYTPEDC RKAFQNPSTV TLMKPSSFTT SFQAISSNIN SDNCLKMLCE

751 VAIPHNRRKE CGQEDLDSTF TICEDIKSSK CKLPEQESLP NDNKDILQRL

801 DPSSFSTKHS MPVPSMVPSY MAMTTAAKRK RKLTSSTSNΞ SLTADVNSGF

851 AKRVRQDNSS EKHLQENKPT MEHKRNICKI NPSMVRKFGR NISKGNLR

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_26g22, frame 1

SWISSPROT :YB3D_SCHPO PUTATIVE KINESIN-LIKE PROTEIN C2F12.13., N = 3, Score = 874, P = 9e-93

TREMBL :DMU89264_1 product: "kinesin like protein 67a"; Drosophila melanogaster kinesin like protein 67a mRNA, complete eds., N = 1, Score = 880, P = 4.2e-88

TREMBL: SPBC649_1 gene: "SPBC649.01c"; product: "putative kmesin-like protein"; S. pombe chromosome II cosmid c649., N = 3, Score = 814, P = 9.8e-86

PIR:S64238 kines n-related protein KIP3 - yeast (Saccharomyces cerevisiae), N = 2, Score = 802, P = 2.5e-83

>TREMBL:DMU89264_1 product: "kinesm like protein 67a"; Drosophila melanogaster kinesm like protein 67a mRNA, complete eds . Length = 814

HSPs :

Score = 880 (132.0 bits), Expect = 4.2e-88, P = 4.2e-88 Identities = 181/345 (52%), Positives = 238/345 (68%)

Query: 11 HMKVVVRVRPENTKEKAAGFHKVVHVVDKHILVFDPKQEEVSFF-HGKKTTNQNVIKKQN 69

++KV VRVRP N +E ++ V+D+ L+FDP +E+ FF G K +++ K+ N Sbjct: 8 NIKVAVRVRPYNVRELEQKQRSIIKVMDRSALLFDPDEEDDEFFFQGAKQPYRDITKRMN 67

Query: 70 KDLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGATGAGKTHTMLGSADE 129

K L FD VFD ++ ++FE T P++ + LNGYNC+V YGATGAGKT TMLGS Sbjct: 68 KKLTMEFDRVFDIDNSNQDLFEECTAPLVDAVLNGYNCSVFVYGATGAGKTFTMLGSEAH 127

Query: 130 PGVMYLTMLHLYKCMDEIKEEKICSTAVSYLEVYNEQIRDLLVNSGPLAVREDTQKGVVV 189

PG+ YLTM L+ + + + VSYLEVYNE + +LL SGPL +RED GVVV Sbjct: 128 PGLTYLTMQDLFDKIQAQSDVRKFDVGVSYLEVYNEHVMNLLTKSGPLKLREDNN-GVVV 186

Query: 190 HGLTLHQPKSSEEILHLLDNGNKNRTQHPTDMNATSSRSHAVFQIYLRQQDKTASINQNV 249

GL L S+EE+L +L GN +RTQHPTD NA SSRSHA+FQ+++R ++ + V Sbjct: 187 SGLCLTPIYSAEELLRMLMLGNSHRTQHPTDANAESΞRSHAIFQVHIRITERKTDTKRTV 246

Query: 250 RIAKMSLIDLAGSERASTSGAKGTRFVEGTNINRSLLALGNVINALADSKRKNQHIPYRN 309

K+S+IDLAGSERA+++ G RF EG +IN+SLLALGN IN LAD + HIPYR+ Sbjct: 247 KLSMIDLAGSERAASTKGIGVRFKEGASINKSLLALGNCINKLADGLK HIPYRD 300

Query: 310 SKLTRLLKDSLGGNCQTIMIAAVΞPSSVFYDDTYNTLKYANRAKDI 355

S LTR+LKDSLGGNC+T+M+A VS SS+ Y+DTYNTLKYA+RAK I Sbjct: 301 SNLTRILKDSLGGNCRTLMVANVSMSSLTYEDTYNTLKYASRAKKI 346

Pedant information for DKFZphtes3_26g22, frame 1

Report for DKFZphtes3_26g22.1

[LENGTH] 898

[MW] 102281.63

[pi] 9.09

[HOMOL] SWISSPROT :YB3D_SCHPO PUTATIVE KINESIN-LIKE PROTEIN C2F12.13. 3e-97

[FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YGL216w] 2e-88

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YGL216w] 2e-88

[FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YGL216w] 2e-88

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YGL216w] 2e-88

[FUNCAT] 09.10 nuclear biogenesis [S. cerevisiae, YPR141c] 5e-42

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YPR141c] 5e-42

[FUNCAT] 03.13 meiosis [S. cerevisiae, YPR141c] 5e-42

[FUNCAT] 11.01 stress response [S. cerevisiae, YPR141c] 5e-42

[ FUNCAT] 03.07 pheromone response, matmg-type determination, sex-specific proteins

[ [S. cerevisiae, YPR141C] 5e-42

[FUNCAT] 30.05 organization of centrosome [S. cerevisiae, YPR141c] 5e-42 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YKL079w] 4e-28 [BLOCKS] BL00411H [BLOCKS] BL00411G [BLOCKS] BL00411F [BLOCKS] BL00411E Kinesin motor domain proteins [BLOCKS] BL00411C Kinesin motor domain proteins [BLOCKS] BL00411B Kinesm motor domain proteins [BLOCKS] BL00411A Kinesin motor domain proteins [SCOP] d2km.l 3.29.1.5.3 Kinesm [Rat (Rattus norvegicus) le-117 [SCOP] d3kar 3.29.1.5.4 Kinesin [Baker's yeast (Ξaccharomyce le-112 [PIRKW] nucleus 6e-87 [PIRKW] heterodimer 4e-68 [PIRKW] DNA binding 9e-60 [PIRKW] heterotetramer 2e-54 [PIRKW] mitosis 9e-60 [PIRKW] microtubule binding 4e-68 [PIRKW] ATP 6e-87 [PIRKW] phosphoprotem 5e-59 [PIRKW] heterotπmer 4e-68 [PIRKW] purme nucleotide binding le-26 [PIRKW] P-loop 6e-87 [PIRKW] colled coil 4e-68 [PIRKW] heptad repeat 3e-62 [PIRKW] methylated ammo acid 2e-54 [PIRKW] hydrolase 2e-54 [PIRKW] GTP binding le-60 [PIRKW] cell division 5e-57

[SUPFAM] kmesin-related protein KIP1 3e-50

[SUPFAM] kmesin-related protein CIN8 7e-33

[SUPFAM] kinesin heavy chain 2e-54

[SUPFAM] suppressor protein SMY1 le-26

[SUPFAM] kmesin-related protein KIF3 4e-68

[SUPFAM] kinesin-related protein KIF2 le-46

[SUPFAM] kinesin-related protein unc-104 7e-60

[SUPFAM] unassigned kmesin-related proteins 6e-87

[SUPFAM] centromere protein E 3e-54

[SUPFAM] kinesin-related protein KLP61F 5e-57

[SUPFAM] kmesin-related protein MKLP-1 2e-28

[SUPFAM] pleckstrm repeat homology 7e-60

[SUPFAM] kinesin-related protein KIF1B 4e-61

[SUPFAM] kinesin motor domain homology 6e-87

[SUPFAM] kmesin-related protein KLPA le-43

[SUPFAM] kinesin-related protein nodA le-30

[SUPFAM] kinesin-related protein Eg5 5e-59

[PROSITE] ATP_GTP_A 1

[PROSITE] KINESIN_MOTOR_DOMAINl 1

[PFAM] Kinesm motor domain

[KW] Irregular

[KW] 3D

[KW] LOW COMPLEXITY 8.57 %

SEQ MSVTEEDLCHHMKVVVRVRPENTKEKAAGFHKVVHVVDKHILVFDPKQEEVSFFHGKKTT SEG 3kar- TBEEE

SEQ NQNVIKKQNKDLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGATGAGKT SEG 3kar- EEEETTTTTTEEEEEETEEETTTTCHHHHHHHHHH-HHHGGGGCCCEEEEEECTTTTCHH

SEQ HTMLGSADEPGVMYLTMLHLYKCMDEIKEEKICSTAVSYLEVYNEQIRDLLVNSGPLAVR SEG 3kar- HHHHTTTT—THHHHHHHHHHHHHHHHGGGCEEEEEEEEEEEETTEEEETT-TCCCCEEE

SEQ EDTQKGVVVHGLTLHQPKSSEEILHLLDNGNKNRTQHPTDMNATΞSRSHAVFQIYLRQQD SEG 3kar- EETTTEEEEETTCCEEECCGGGHHHHHHHHHHHHCCTTTTCHHHHHHCEEEEEEEEEEEE

SEQ KTASINQNVRIAKMSLIDLAGSERASTSGAKGTRFVEGTNINRSLLALGNVINALADSKR SEG 3kar- TTTTCEE EEEEEEEECCCCCCCCCC HHHHHHHHHHHHHHHHHHHHHHHHTTTT

SEQ KNQHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSVFYDDTYNTLKYANRAKDIKSSLK

SEG xxxxx

3kar- TTTCCTTTTTHHHHHHGGGCTTTTEEEEEEEECCCGGGHHHHHHHHHHHHH

SEQ SNVLNVNNHITQYVKICNEQKAEILLLKEKLKAYEEQKAFTNENDQAKLMISNPQEKEIE SEG xxxxxxxx xxxxxxxxxxxxxxxxxxxxx 3kar-

SEQ RFQEILNCLFQNREEIRQEYLKLEMLLKENELKSFYQQQCHKQIEMMCSEDKVEKATGKR SEG xxxxxxxxxxxxx 3kar-

SEQ DHRLAMLKTRRSYLEKRREEELKQFDENTNWLHRVEKEMGLLSQNGHIPKELKKDLHCHH SEG xxxxxxxxxxx 3kar-

SEQ LHLQNKDLKAQIRHMMDLACLQEQQHRQTEAVLNALLPTLRKQYCTLKEAGLSNAAFESD SEG XXX 3kar-

SEQ FKEIEHLVERKKVVVWADQTAEQPKQNDLPGISVLMTFPQLGPVQPIPCCSSSGGTNLVK SEG 3kar-

SEQ IPTEKRTRRKLMPSPLKGQHTLKSPPSQSVQLNDSLSKELQPIVYTPEDCRKAFQNPSTV SEG 3kar-

SEQ TLMKPSSFTTSFQAISSNINSDNCLKMLCEVAIPHNRRKECGQEDLDSTFTICEDIKSSK SEG 3kar-

SEQ CKLPEQESLPNDNKDILQRLDPSSFSTKHSMPVPSMVPSYMAMTTAAKRKRKLTSSTSNS SEG XXXXXXXXXXXXX 3kar- SEQ SLTADVNSGFAKRVRQDNSSEKHLQENKPTMEHKRNICKINPSMVRKFGRNISKGNLR SEG XXX 3kar-

Prosite for DKFZphtes3_26g22.1

PS00017 113->121 ATP_GTP_A PDOC00017 PΞ00411 252->264 KINESIN MOTOR DOMAIN1 PDOC00343

Pfam for DKFZphtes3_26g22.1

HMM_NAME Kinesin motor domain

HMM *RCRPlNeREιndgcscvVQWPpWtGyktvhnghegds

R+RP N +E+++G +VV + + + + +++E S

Query 17 RVRPENTKEKAAGFHKVVHVVD-KHILVFDPKQEEVSFFHGKKTTNQNV 64

HMM phksFtFDHVFWWncTQedVYdtvAHPIVDDcFhGYNCTIFAYGQ

+ F+FD VF+ ++TQ +V++ + PI+ ++++GYNCT++AYG

Query 65 IKKQNKDLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGA 114

HMM TGSGKTYTMMGpggehPDHmGIIPRcCHDIFdrldkfqekDhdFWhVkCS TG+GKT+TM G + D+ G+ + +++++ D + + + +S

Query 115 TGAGKTHTMLG SADEPGVMYLTMLHLYKCMDEIK-EEKIC-STAVS 158

HMM YMEIYNEelYDLLCPnPqhMkpLnlHEHPNMGpYVqGCTEfHVcSYeDac Y+E+YNE+I+DLL+ N ++PL+++E+ G+ V G+T+ +S E+++

Query 159 YLEVYNEQIRDLLV-N SGPLAVREDTQKGVVVHGLTLHQPKSSEEIL 204

HMM hWIWqGnknRHVAaTnMNdhSSRSHtlFTIHVeQrHk..qcdehvcHSKM H+++ GNKNR+ +T MN++SSRSH++F+I ++Q K + V++ KM

Query 205 HLLDNGNKNRTQHPTDMNATSSRSHAVFQIYLRQQDKTASINQNVRIAKM 254

HMM NLVDLAGSERvnrTGAEGQRIKEGcNINqSLttLGnVInaLaDgqTKYmY +L+DLAGSER++ +GA G+R+ EG+NIN+SL++LGNVINALAD +

Query 255 SLIDLAGSERASTSGAKGTRFVEGTNINRSLLALGNVINALADSK 299

HMM gghgHIPYRDSKLTWILQDSLGGNcKTcMIACIWPadWNYEETLΞTLRYA +++HIPYR SKLT+LL+DSLGGNC T MIA+++P+ + Y++T +TL+YA

Query 300 RKNQHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSVFYDDTYNTLKYA 349

HMM dRAKnlkNkPQINEDPcamalWRrYheQIqdMKhqL* +RAK+IK + N + + + +Y + + K++

Query 350 NRAKDIKSSLKSNVLNVN-NHITQYVKICNEQKAEI 384

DKFZphtes3_27dl

group: metabolism

DKFZphtes3_27dl encodes a novel 712 amino acid protein similar to ubiquitm-specific proteases (EC 3.1.2.15) .

The novel protein contains both, a ubiquitin carboxyl-terminal hydrolases family 2 signature 1 and signature 2. Pfam predicts a new member of the ubiquitin carboxyl-terminal hydrolases family 2. The ubiquitin system is responsible for the turn over of proteins. Ubiquitin carboxyl-terminal hydrolases (EC 3.1.2.15) (UCH) (deubiquitinatmg enzymes) are thiol proteases that recognize and hydrolyze the peptide bond at the C-terimnal glycine of ubiquitin. These enzymes are involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins.

The novel protein is a new member of the ubiquitin carboxyl-terminal hydrolases family 2, represented by proteins such as yeast UBP1-16, human tre-2, human isopeptidase T and others.

The novel protein can find application m modulation of ubiquitin- and protein metabolism in cells . similarity to ubiquitm-specific proteases complete cDNA, complete eds, 4 EST hits

Sequenced by GBF

Locus : unknown

Insert length: 2871 bp

Poly A stretch at pos. 2836, no polyadenylation signal found

1 CCAAACCTGA AAGAGGTTGA TTTGTAATGA TTTGCAGGGG GGCACTGGAG

51 GCAGCGGCCA GGACTTTTCA CTTAGGAGAT CAGCATTTGC CCTGATGGAA

101 ACTGGGCGAT CCTGCAGGGA CTGACCTCTG AGTTATCCAA AGGCCGACCT

151 GGGGAAAGAC TGATTTTGAG GTTTTAATAG TTTTCAGATG CTTCAAGTGT

201 TGTGAACAGA GACTTGTTTG GATTATGCAT TTCTCAGCTA GACTAAATAA

251 ATGCTAGCAA TGGATACGTG CAAACATGTT GGGCAGCTGC AGCTTGCTCA

301 AGACCATTCC AGCCTCAACC CTCAGAAATG GCACTGTGTG GACTGCAACA

351 CGACCGAGTC CATTTGGGCT TGCCTTAGCT GCTCCCATGT TGCCTGTGGA

401 AGATATATTG AAGAGCATGC ACTCAAGCAC TTTCAAGAAA GCAGTCATCC

451 TGTTGCATTG GAGGTGAATG AGATGTACGT TTTTTGTTAC CTTTGTGATG

501 ATTATGTTCT GAATGATAAC GCAACTGGAG ACCTGAAGTT ACTACGACGT

551 ACATTAAGTG CCATCAAAAG TCAAAATTAT CACTGCACAA CTCGTAGTGG

601 GAGGTTTTTA CGGTCCATGG GTACAGGTGA TGATTCTTAT TTCTTACATG

651 ACGGTGCCCA ATCTCTGCTT CAAAGTGAAG ATCAACTGTA TACTGCTCTT

701 TGGCACAGGA GAAGGATACT AATGGGTAAA ATCTTTCGAA CATGGTTTGA

751 ACAATCACCC ATTGGAAGAA AAAAGCAAGA AGAACCATTT CAGGAGAAAA

801 TAGTAGTAAA AAGAGAAGTA AAGAAAAGAC GGCAGGAATT GGAGTATCAA

851 GTTAAAGCAG AATTGGAAAG TATGCCTCCA AGAAAGAGTT TACGTTTACA

901 AGGGCTCGCT CAGTCGACCA TAATAGAAAT AGTTTCTGTT CAGGTGCCAG

951 CACAAACGCC AGCATCACCA GCAAAAGATA AAGTACTCTC TACCTCAGAA

1001 AATGAAATAT CTCAAAAAGT CAGTGACTCC TCAGTTAAAC GAAGGCCAAT

1051 AGTAACTCCT GGTGTAACAG GATTGAGAAA TTTGGGAAAT ACTTGCTATA

1101 TGAATTCTGT TCTTCAGGTG TTGAGTCATT TACTTATTTT TCGACAATGT

1151 TTTTTAAAGC TTGATCTGAA CCAATGGCTG GCTATGACTG CTAGCGAGAA

1201 GACAAGATCT TGTAAGCATC CACCAGTCAC AGATACAGTA GTATATCAAA

1251 TGAATGAATG TCAGGAAAAA GATACAGGTT TTGTTTGCTC CAGACAATCA

1301 AGTCTGTCAT CAGGACTAAG TGGTGGAGCA TCAAAAGGTA GAAAGATGGA

1351 ACTTATTCAG CCAAAGGAGC CAACTTCACA GTACATTTCT CTTTGTCATG

1401 AATTGCATAC TTTGTTCCAA GTCATGTGGT CTGGAAAGTG GGCGTTGGTC

1451 TCACCATTTG CTATGCTACA CTCAGTGTGG AGACTCATTC CTGCCTTTCG

1501 TGGTTACGCC CAACAAGACG CTCAGGAATT TCTTTGTGAA CTTTTAGATA

1551 AAATACAACG TGAATTAGAG ACAACTGGTA CCAGTTTACC AGCTCTTATC

1601 CCCACTTCTC AAAGGAAACT CATCAAACAA GTTCTGAATG TTGTAAATAA

1651 CATTTTTCAT GGACAACTTC TTAGTCAGGT TACATGTCTT GCATGTGACA

1701 ACAAATCAAA TACCATAGAA CCTTTCTGGG ACTTGTCATT GGAGTTTCCA

1751 GAAAGGTATC AATGCAGTGG AAAAGATATT GCTTCCCAGC CATGTCTGGT

1801 TACTGAAATG TTGGCCAAAT TTACAGAAAC TGAAGCTTTA GAAGGAAAAA

1851 TCTACGTATG TGACCAGTGT AACTCAAAGC GTAGAAGGTT TTCCTCCAAA

1901 CCAGTTGTAC TCACAGAAGC CCAGAAACAA CTTATGATAT GCCACCTACC

1951 TCAGGTTCTC AGACTGCACC TCAAACGATT CAGGTGGTCA GGACGTAATA

2001 ACCGAGAGAA GATTGGTGTT CATGTTGGCT TTGAGGAAAT CTTAAACATG

2051 GAGCCCTATT GCTGCAGGGA GACCCTGAAA TCCCTCAGAC CAGAATGCTT

2101 TATCTATGAC TTGTCCGCGG TGGTGATGCA CCATGGGAAA GGATTTGGCT

2151 CAGGGCACTA CACTGCCTAC TGCTATAATT CTGAAGGAGG GTTCTGGGTA

2201 CACTGCAATG ATTCCAAACT AAGCATGTGC ACTATGGATG AAGTATGCAA

2251 GGCTCAAGCT TATATCTTGT TTTATACCCA ACGAGTTACT GAGAATGGAC 2301 ATTCTAAACT TTTGCCTCCA GAGCTCCTGT TGGGGAGCCA ACATCCCAAT

2351 GAAGACGCTG ATACCTCGTC TAATGAAATC CTTAGCTGAT CCAAAGACAA

2401 TGGGGTTTTC TTCCTGTGAT TTATATATAT ACTTTTTAAA AGACTGATGT

2451 ACCATTTTAA ACTTCATTTT TTCTTGTGAA TCAGTGTATA CTACATTTAT

2501 ACATTTTATA TCTAACAATT TTTTTTTTTT ACAAAGTATA AATGTATATA

2551 TCAACTGAAG GTAACTACTT TTTTCATATT TGGAGTTTTA AACTTTTGGT

2601 GTTTACCTCA GACTGATGTT ACCTCTTTTA TATTTTTATG TCTTAATTGG

2651 CTCGGATGAT GAACTTGTGC AATCTTCTAC CAACAAAGTT CAAGTGGCAT

2701 CATTTTATAT ACATGTATCT TTTTCAGGTA TTTTCTATAC AAATTCTTAA

2751 TAGATGGAAA ATTAGACTCT AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

2801 AAAAAAAAAA AAAAAAAAAA AAGGGGCGGC CGCTCTAAAA AAAAAAAAAA

2851 AAAAAAAAAA AAAAAAAAAG G

BLAST Results

No BLAST result

Medline entries

98072201:

Regulation of ubiquitin-dependent processes by deubiquitmating enzymes .

98431658:

The ubiquitin system.

Peptide information for frame 2

ORF from 251 bp to 2386 bp; peptide length: 712 Category: similarity to known protein Prosite motifs: UCH_2_1 (274-290) UCH_2_2 (619-638) UCH 2 2 (619-638)

1 MLAMDTCKHV GQLQLAQDHS SLNPQKWHCV DCNTTESIWA CLSCSHVACG 51 RYIEEHALKH FQESSHPVAL EVNEMYVFCY LCDDYVLNDN ATGDLKLLRR 101 TLSAIKSQNY HCTTRSGRFL RSMGTGDDSY FLHDGAQSLL QSEDQLYTAL 151 WHRRRILMGK IFRTWFEQSP IGRKKQEEPF QEKIVVKREV KKRRQELEYQ 201 VKAELESMPP RKSLRLQGLA QSTIIEIVSV QVPAQTPASP AKDKVLSTSE 251 NEISQKVSDS SVKRRPIVTP GVTGLRNLGN TCYMNSVLQV LΞHLLIFRQC 301 FLKLDLNQWL AMTASEKTRS CKHPPVTDTV VYQMNECQEK DTGFVCSRQS 351 SLSSGLSGGA SKGRKMELIQ PKEPTSQYIS LCHELHTLFQ VMWSGKWALV 401 SPFAMLHSVW RLIPAFRGYA QQDAQEFLCE LLDKIQRELE TTGTSLPALI 451 PTSQRKLIKQ VLNVVNNIFH GQLLSQVTCL ACDNKSNTIE PFWDLSLEFP 501 ERYQCSGKDI ASQPCLVTEM LAKFTETEAL EGKIYVCDQC NSKRRRFSSK 551 PVVLTEAQKQ LMICHLPQVL RLHLKRFRWS GRNNREKIGV HVGFEEILNM 601 EPYCCRETLK SLRPECFIYD LSAVVMHHGK GFGSGHYTAY CYNSEGGFWV 651 HCNDSKLSMC TMDEVCKAQA YILFYTQRVT ENGHSKLLPP ELLLGSQHPN 701 EDADTSSNEI LS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_27dl, frame 2

PIR:S57591 hypothetical protein YMR223w - yeast (Saccharomyces cerevisiae), N = 4, Score = 218, P = 8.4e-38

SWISSPROT :UBPB_HUMAN UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 11 (EC 3.1.2.15) (UBIQUITIN THIOLESTERASE 11) (UBIQUITIN-SPECIFIC PROCESSING PROTEASE 13) (DEUBIQUITINATING ENZYME 11) (KIAA0055)., N = 2, Score = 300, P = 9.3e-31

TREMBL:AF079565_1 gene: "Ubp41"; product: "ubiquitm-specific protease UBP41"; Mus musculus ubiquitm-specific protease UBP41 (Ubp41) mRNA, complete eds., N = 3, Score = 187, P = 8.7e-30

PIR: 158376 hypothetical protein unp - mouse, N = 3, Score = 214, P = 1.2e-28 >SWISSPROT:UBPB_HUMAN UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 11 (EC 3.1.2.15) (UBIQUITIN THIOLESTERASE 11) (UBIQUITIN-SPECIFIC PROCESSING PROTEASE 13) (DEUBIQUITINATING ENZYME 11) (KIAA0055) . Length = 1,118

HSPs:

Score = 300 (45.0 bits), Expect = 9.3e-31, Sum P(2) = 9.3e-31 Identities = 95/301 (31%), Positives = 149/301 (49%)

Query: 381 LCHELHTLFQVMWΞGKWALVSPFAMLHSVWRLIPAFRGYAQQDAQEFLCELLDKIQREL- 439

+ E + + +W+G++ +SP ++ ++ F GY+QQD+QE L L+D + +L Sbjct: 826 VAEEFGIIMKALWTGQYRYISPKDFKITIGKINDQFAGYSQQDSQELLLFLMDGLHEDLN 885

Query: 440 ETTGTSLPALIPTSQRKLIKQVLN—VVNNIFHGQLLSQVTCLACDNKSNT 488

E L + LN ++ +F GQ S V CL C KS T

Sbjct: 886 KADNRKRYKEENNDHLDDFKAAEHAWQKHKQLNESIIVALFQGQFKΞTVQCLTCHKKSRT 945

Query: 489 IEPFWDLSLEFPERYQCSGKDIASQPCLVTEMLAKFTETEALEGKIYVCDQCNSKRRRFS 548

E F LSL +C+ +D CL + +K E + + + C C ++R Sbjct: 946 FEAFMYLSLPLASTSKCTLQD CL--RLFSK—EEKLTDNNRFYCSHCRARR 992

Query: 549 SKPVVLTEAQKQLMICHLPQVLRLHLKRFRWSGRNNREKIGVHVGFE-EILNMEPYCC— 605

++ K++ I LP VL +HLKRF + GR ++K+ V F E L++ Y Sbjct: 993 DSLKKIEIWKLPPVLLVHLKRFSYDGRW-KQKLQTSVDFPLENLDLSQYVIGP 1044

Query: 606 RETLKSLRPECFIYDLSAVVMHHGKGFGSGHYTAYCYNSEGGFWVHCNDSKLSMCTMDEV 665

+ LK Y+L +V H+G G GHYTAYC N+ W +D ++S ++ V Sbjct: 1045 KNNLKK YNLFSVSNHYG-GLDGGHYTAYCKNAARQRWFKFDDHEVSDISVSSV 1096

Query: 666 CKAQAYILFYTQ RVTE 681

+ AYILFYT RVT+ Sbjct: 1097 KSΞAAYILFYTSLGPRVTD 1115

Score = 126 (18.9 bits), Expect = 9.3e-31, Sum P(2) = 9.3e-31 Identities = 41/116 (35%), Positives = 63/116 (54%)

Query: 200 QVKAELESMPPR—KSLRLQGLAQSTIIEIVSVQVPAQTPASPAKDKVLSTSENEISQKV 257

Q+ AE + P + +S + Q+ 1+ + P TP ++K + EIS ++ Sbjct: 701 QIPAERDREPSKLKRSYSSPDITQA—IQEEEKRKPTVTPTVNRENKPTCYPKAEIS-RL 757

Query: 258 SDΞSVKR-RPIVT PGVTGLRNLGNTCYMNSVLQVLS HLLIF--RQCFLKLDLNQ 308

S S ++ P+ P +TGLRNLGNTCYMNS+LQ L HL + R C+ D+N+ Sbjct: 758 ΞASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQD-DINR 816

Score = 50 (7.5 bits), Expect = 8.3e-23, Sum P(2) = 8.3e-23 Identities = 29/106 (27%), Positives = 51/106 (48%)

Query: 173 RKKQEEPFQEKIVVKREVKKRRQELEYQVKAELESMPPRKSLRLQGLAQSTIIEIVSVQV 232

+ KQE+ +E+ +++ K R++E E + K + E+ + Q A+ + + S Q Sbjct: 475 KNKQEKELRERQQEEQKEKLRKEEQEQKAKKKQEA-EENEITEKQQKAKEEMEKKESEQA 533

Query: 233 PAQ TPASPAKD KVLSTSENEIS—QKVΞDSSVKRRPIVTPGV 272

+ T A K+ K S SE+E S +K + KR P TP + Sbjct: 534 KKEDKETSAKRGKEITGVKRQSKSEHETSDAKKSVEDRGKRCP—TPEI 580

Score = 42 (6.3 bits), Expect = 5.7e-22, Sum P(2) = 5.7e-22 Identities = 13/58 (22%), Positives = 27/58 (46%)

Query: 167 EQSPIGRKKQEEPFQEKIVVKREVKKRRQELEY-QVKAELESMPPRKSLRLQGLAQΞT 223

EQ +KKQE E +++ K+ ++ E Q K E + ++ + G+ + + Sbjct: 498 EQEQKAKKKQEAEENEITEKQQKAKEEMEKKESEQAKKEDKETΞAKRGKEITGVKRQS 555

Pedant information for DKFZphtes3_27dl, frame 2

Report for DKFZphtes3_27dl .2

[LENGTH] 712

[MW] 81155.71

[pi] 8.21

[HOMOL] SWISSPROT :UBPB_HUMAN UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 11 (EC 3.1.2.15)

(UBIQUITIN THIOLESTERASE 11) (UBIQUITIN-SPECIFIC PROCESSING PROTEASE 13) (DEUBIQUITINATING ENZYME 11) (KIAA0055) . 4e-32

[FUNCAT] 06.13.01 cytoplasmic degradation [S. cerevisiae, YMR223w] 5e-33

[FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YMR223w] 5e-33 [ FUNCAT ] 06.13 proteolysis [S. cerevisiae, YBL067c] 3e-19

[ FUNCAT ] 10.03.99 other osmosensing activities [S. cerevisiae, YDR069c] 2e-17

[FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YDR069c] 2e-17

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YDR069c] 2e-17

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDR069c] 2e-17

[FUNCAT] 09.25 vacuolar and lysosomal biogenesis [S. cerevisiae, YDR069c] 2e-17

[FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YNL186w] 4e-17

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YHLOlOc] 3e-12

[BLOCKS] BL00970A Nuclear transition protein 2 proteins

[BLOCKS] BL00972D

[BLOCKS] BL00972C

[BLOCKS] BL00972B

[BLOCKS] BL00972A

[EC] 3.1.2.15 Ubiquitin thiolesterase 5e-06

[PIRKW] alternative splicing 2e-ll

[PIRKW] thiolester hydrolase 5e-06

[PIRKW] hydrolase le-14

[SUPFAM] RING finger homology 7e-ll

[SUPFAM] deubiqumatmg enzyme SSV7 5e-16

[PROSITE] MYRISTYL 5

[PROSITE] AMIDATION 2

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 10

[PROSITE] TYR_PHOSPHO_SITE 2

[PROSITE] UCH_2_21

[PROSITE] PKC_PHOSPHO_SITE 17

[PROSITE] ASN_GLYCOSYLATION 4

[PROSITE] UCH_2_11

[PFAM] Ubiquitin carboxyl-terminal hydrolases family 2

[PFAM] Ubiquitin carboxyl-terminal hydrolases family 2

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 4.92 %

SEQ MLAMDTCKHVGQLQLAQDHSSLNPQKWHCVDCNTTESIWACLSCSHVACGRYIEEHALKH SEG PRD ccccccccchhhhhhhhcccccccccceeecccceeeeeeeccccccccchhhhhhhhhh

SEQ FQESSHPVALEVNEMYVFCYLCDDYVLNDNATGDLKLLRRTLSAIKSQNYHCTTRSGRFL SEG PRD hhhhccceeecccceeeeeeccccccccccccchhhhhhhhhhhhhcccceeeccccccc

SEQ RSMGTGDDSYFLHDGAQSLLQSEDQLYTALWHRRRILMGKIFRTWFEQSPIGRKKQEEPF SEG PRD cccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhchhhhhhhhhhh

SEQ QEKIVVKREVKKRRQELEYQVKAELESMPPRKΞLRLQGLAQSTIIEIVSVQVPAQTPASP SEG xxxxxxxxxxxxxxxx PRD hheeehhhhhhhhhhhhhhhhhhhhhhcccccccccccccccceeeeeeccccccccccc

SEQ AKDKVLSTΞENEISQKVSDSSVKRRPIVTPGVTGLRNLGNTCYMNSVLQVLSHLLIFRQC SEG PRD ccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhh

SEQ FLKLDLNQWLAMTASEKTRSCKHPPVTDTVVYQMNECQEKDTGFVCSRQSSLSSGLSGGA SEG xxxxxxxxxxxxxx PRD hhhhhhchhhhhhhhhhhhhhccccccceeehhhhhcccccccccccccccccccccccc

SEQ SKGRKMELIQPKEPTSQYISLCHELHTLFQVMWSGKWALVSPFAMLHSVWRLIPAFRGYA SEG xxxxx PRD ccccceeecccccccchhhhhhhhhhhhhhhhhccceeeeccchhhhhhhhhhhccccch

SEQ QQDAQEFLCELLDKIQRELETTGTSLPALIPTSQRKLIKQVLNVVNNIFHGQLLSQVTCL

SEG PRD hhhhhhhhhhhhhhhhhhhhhhccccccccccchhhhhhhhhhhhhccccchhhhhhhhc

SEQ ACDNKSNTIEPFWDLSLEFPERYQCSGKDIASQPCLVTEMLAKFTETEALEGKIYVCDQC SEG PRD cccccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhccceeecccc

SEQ NSKRRRFSSKPVVLTEAQKQLMICHLPQVLRLHLKRFRWSGRNNREKIGVHVGFEEILNM SEG PRD ccccccccccchhhhhhhhhhhhhhchhhhhhhhhhhhhcccccccccceeeeccccccc

SEQ EPYCCRETLKSLRPECFIYDLSAVVMHHGKGFGSGHYTAYCYNSEGGFWVHCNDSKLSMC SEG PRD ccccccccccccccceeeeeeeeeeeecccccccccceeeeccccccceeeecccccccc

SEQ TMDEVCKAQAYILFYTQRVTENGHSKLLPPELLLGSQHPNEDADTSSNEILS SEG PRD cchhhhhhhhhhhhhheeeecccccccccccccccccccccccccccccccc

DKFZphtes3_27k4

group: transmembrane protein

Summary DKFZphtes3_27k4 encodes a novel 490 ammo acid protein with similarity to two hypothetical C. elegans proteins.

The novel protein contains 10 transmembrane regions and a leucine zipper. It is a member of the new 10 trans-membrane domain containing protein family which is specific for multicellular eukariotes . No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes and as a new marker for testicular cells. strong similarity to C. elegans K07H8.2/ZK185.2 membrane regions: 10 complete cDNA, complete eds potential start at Bp 109, few EST hits

Sequenced by GBF

Locus : unknown

Insert length: 1901 bp

Poly A stretch at pos. 1866, no polyadenylation signal found

1 GTGATTTACC AGAAAAACCA AGAAGACAGG CACAAAAAAG CAAACGGCAT

51 TTGGCAAGAT GGATTATCAA CTGCAGTACA GACTTTTAGT AATAGATCTG

101 AGCAACACAT GGAGTATCAC AGTTTCTCAG AGCAGTCTTT TCATGCCAAT

151 AATGGGCACG CATCATCAAG CTGCAGCCAA AAGTATGATG ACTATGCCAA

201 TTATAATTAC TGTGATGGAA GGGAGACTTC AGAAACCACT GCCATGTTAC

251 AAGATGAAGA TATATCTAGT GATGGTGATG AAGATGCTAT TGTAGAAGTG

301 ACCCCAAAAT TACCAAAGGA ATCCAGTGGC ATCATGGCAT TGCAAATACT

351 TGTGCCCTTT TTGCTAGCTG GTTTTGGAAC AGTTTCAGCT GGCATGGTAC

401 TGGATATAGT ACAGCACTGG GAGGTGTTCA GAAAAGTTAC AGAAGTTTTC

451 ATTTTAGTCC CTGCACTTCT TGGTCTCAAA GGGAACTTGG AAATGACATT

501 GGCATCCAGA TTATCCACTG CAGTAAATAT TGGGAAGATG GATTCACCCA

551 TTGAAAAGTG GAACCTAATA ATTGGCAACT TGGCTTTAAA GCAGGTTCAG

601 GCAACAGTAG TGGGTTTTCT AGCAGCTGTG GCAGCAATTA TATTGGGCTG

651 GATTCCAGAA GGAAAATATT ACCTTGATCA TTCCATACTT CTGTGCTCTA

701 GCAGTGTGGC AACTGCCTTC ATTGCATCTC TTCTGCAGGG AATAATAATG

751 GTTGGGGTTA TCGTTGGTTC AAAGAAGACT GGTATAAATC CTGATAATGT

801 TGCTACACCC ATTGCTGCTA GTTTTGGCGA CCTTATAACT CTTGCCATAT

851 TGGCTTGGAT AAGTCAGGGC TTATACTCCT GTCTTGAGAC CTATTACTAC

901 ATTTCTCCAT TAGTTGGTGT ATTTTTCTTG GCTCTAACCC CTATTTGGAT

951 TATAATAGCT GCCAAACATC CAGCCACAAG AACAGTTCTC CACTCAGGCT

1001 GGGAGCCTGT CATAACAGCT ATGGTTATAA GTAGCATTGG GGGCCTTATT

1051 CTGGACACAA CTGTATCAGA CCCAAACTTG GTTGGGATTG TTGTTTACAC

1101 GCCAGTTATT AATGGTATTG GTGGTAATTT GGTGGCCATT CAGGCTAGCA

1151 GGATTTCTAC CTACCTCCAT TTACATAGCA TTCCAGGAGA ATTGCCTGAT

1201 GAACCCAAAG GTTGTTACTA CCCATTTAGA ACTTTCTTTG GTCCAGGAGT

1251 AAATAATAAG TCTGCTCAAG TTCTACTGCT TTTAGTGATT CCTGGACATT

1301 TAATTTTCCT CTACACTATT CATTTGATGA AAAGTGGTCA TACTTCTTTA

1351 ACTATAATCT TCATAGTAGT GTATTTATTT GGCGCTGTGT TACAGGTATT

1401 TACCTTGCTG TGGATTGCTG ACTGGATGGT CCATCACTTC TGGAGGAAAG

1451 GAAAGGACCC GGATAGTTTC TCCATCCCCT ACCTAACAGC ATTGGGTGAT

1501 CTGCTCGGGA CAGCTCTGTT AGCCTTAAGT TTTCATTTTC TTTGGCTTAT

1551 TGGAGATCGA GATGGAGATG TTGGAGACTA ATAAATTCTA CAAACTGCTC

1601 TCAAGTTACC AAGGAAGAAA ATACACGACA ACCACTTATG GCTCTTTTTC

1651 AAAACTCTTA AATCAGTAGT TTGACTTTTG CCAGGGTAAT CTTCAGTTGG

1701 CCCTGATTCA ATTAAATGGC CTTAATTTTT TTTTAAGGAA TTTGTGTCAA

1751 AACCAGAATG AAGAGTATTC GTGCTGCTTT TCATAGAATA AATGATAATT

1801 TGACATAGAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

1851 AAAAAAAAAA AAGGGGAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAGGG

1901 G

BLAST Results o BLAST result

Medline entries No Medlme entry

Peptide information for frame 1

ORF from 109 bp to 1578 bp; peptide length: 490 Category: similarity to unknown protein

1 MEYHΞFSEQS FHANNGHASS SCSQKYDDYA NYNYCDGRET SETTAMLQDE

51 DISSDGDEDA IVEVTPKLPK ESSGIMALQI LVPFLLAGFG TVSAGMVLDI

101 VQHWEVFRKV TEVFILVPAL LGLKGNLEMT LASRLSTAVN IGKMDSPIEK

151 WNLIIGNLAL KQVQATVVGF LAAVAAIILG WIPEGKYYLD HSILLCSSSV

201 ATAFIASLLQ GIIMVGVIVG SKKTGINPDN VATPIAASFG DLITLAILAW

251 ISQGLYSCLE TYYYISPLVG VFFLALTPIW IIIAAKHPAT RTVLHSGWEP

301 VITAMVISSI GGLILDTTVS DPNLVGIVVY TPVINGIGGN LVAIQASRIS

351 TYLHLHSIPG ELPDEPKGCY YPFRTFFGPG VNNKSAQVLL LLVIPGHLIF

401 LYTIHLMKSG HTSLTIIFIV VYLFGAVLQV FTLLWIADWM VHHFWRKGKD

451 PDSFSIPYLT ALGDLLGTAL LALSFHFLWL IGDRDGDVGD

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_27k4, frame 1

TREMBL :AF036704_2 gene: "ZK185.2"; Caenorhabditis elegans cosmid ZK185., N = 1, Score = 730, P = 3.1e-72

TREMBL:AF047659_9 gene: "K07H8.2"; Caenorhabditis elegans cosmid K07H8., N = 1, Score = 940, P = 1.7e-94

>TREMBL:AF047659_9 gene: "K07H8.2"; Caenorhabditis elegans cosmid K07H8. Length = 507

HSPs:

Score = 940 (141.0 bits), Expect = 1.7e-94, P = 1.7e-94 Identities = 204/412 (49%), Positives = 271/412 (65%)

Query: 68 LPKESSGIMALQILVPFLLAGFGTVSAGMVLDIVQHWEVFRKVTEVFILVPALLGLKGNL 127

+P ESS ++ Q+L PF +AG G V AG+VL IV W +F ++ E+ ILVPALLGLKGNL Sbjct: 82 IPAESSYVLFFQVLFPFAVAGLGMVFAGLVLSIVVTWPLFEEIPEILILVPALLGLKGNL 141

Query: 128 EMTLASRLSTAVNIGKMDSPIEKWNLIIGNLALKQVQATVVGFLAAVAAIILGWIPEGKY 187

EMTLASRLST N+G MDS ++ +++I NLAL QVQATVV FLA+ A L +IP G + Sbjct: 142 EMTLASRLSTLANLGHMDSSKQRKDVVIANLALVQVQATVVAFLASAFAAALAFIPSGDF 201

Query: 188 YLDHSILLCSSSVATAFIASLLQGIIMVGVIVGSKKTGINPDNVATPIAASFGDLITLAI 247

H L+C+SS+ATA ASL+ ++MV VIV S+K INPDNVATPIAAΞ GDL TL + Sbjct: 202 DWAHGALMCASSLATACSASLVLSLLMVVVIVTSRKYNINPDNVATPIAASLGDLTTLTV 261

Query: 248 LAWISQGLYSCLETYYYISPLVGVFFLALTPIWIIIAAKHPATRTVLHSGWEPVITAMVI 307

LA+ T +++ +V V FL L P WI IA ++ T+ L++GW PVI +M+I

Sbjct: 262 LAFFGSVFLKAHNTEΞWLNVIVIVLFLLLLPFWIKIANENEGTQETLYNGWTPVIMSMLI 321

Query: 308 SSIGGLILDTTVSDPNLVGIVVYTPVINGIGGNLVAIQASRISTYLHLHSIPGELPDEPK 367

SS GG IL+T V + + Y PV+NG+GGNL A+QASR+STY H G LP+E Sbjct: 322 SSAGGFILETAVRRYH--SLSTYGPVLNGVGGNLAAVQASRLSTYFHKAGTVGVLPNEWT 379

Query: 368 GCYYPF—RTFFGPGVNNKSAQVLLLLVIPGHLIFLYTIHLM KSGHTSLTIIFIVV 421

+ R FF +++SA+VLLLLV+PGH+ F + I L K+ T +F + Sbjct: 380 VSRFTSVQRAFFSKEWDSRSARVLLLLVVPGHICFNFLIQLFTLTSKNNVTPHGPLFTSL 439

Query: 422 YLFGAVLQVFTLLWIADWMVHHFWRKGKDPDSFSIPYLTALGDLLGTALLALSF 475

Y+ A++QV LL++ +V W+ DPD+ IPYLTALGDLLGT LL + F Sbjct: 440 YMIAAIIQVVILLFVCQLLVALLWKWKIDPDNSVIPYLTALGDLLGTGLLFIVF 493

Pedant information for DKFZphtes3_27k4, frame 1

Report for DKFZphtes3_27k4.1

[LENGTH] 490

[MW] 53266.39 [pi] 5.29 [HOMOL] TREMBL :AF047659_9 gene: "K07H8.2"; Caenorhabditis elegans cosmid K07H8. 4e-94

[PROSITE] LEUCINE_ZIPPER 1

[PROSITE] MYRISTYL 7

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 7

[PROSITE] PROKAR_LIPOPROTEIN 2

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 3

[PROSITE] ASN_GLYCOSYLATION 1

[KW] TRANSMEMBRANE 10

[KW] LOW COMPLEXITY 3.06

SEQ MEYHSFSEQSFHANNGHASSSCSQKYDDYANYNYCDGRETSETTAMLQDEDISSDGDEDA SEG PRD cccccccceeeccccccccccccccccccceeecccccccchhhhhhhhcccccccccee

MEM

SEQ IVEVTPKLPKESSGIMALQILVPFLLAGFGTVΞAGMVLDIVQHWEVFRKVTEVFILVPAL SEG PRD eeeeeccccccchhhhhhhhhhhhhhhcccchhhhhhhhhcchhhhhcccceeeeeeccc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMM . MMMMMMMMMMMMMMM

SEQ LGLKGNLEMTLASRLSTAVNIGKMDSPIEKWNLIIGNLALKQVQATVVGFLAAVAAIILG SEG PRD ccccchhhhhhhhhhhhhhccccccccccceeeehhhhhhhhhhhhhhhhhhhhhhhhhh

MEM MMMMMMM MMMMMMMMMMMMMMMMM

SEQ WIPEGKYYLDHSILLCSSSVATAFIASLLQGIIMVGVIVGSKKTGINPDNVATPIAASFG SEG PRD hcccceeecccceeehhhhhhhhhhhhhhhhhhhhheeeecccccccccccccccccccc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM MMMMMM

SEQ DLITLAILAWISQGLYSCLETYYYISPLVGVFFLALTPIWIIIAAKHPATRTVLHSGWEP SEG PRD cchhhhhhhhhhhhhhhhcceeeeehhhhhhhhhhchhhhhhhhccccccccchhhhhhh MEM MMMMMMMMMMMMMMM....MMMMMMMMMMMMMMMMMMMMM MMMMMM

SEQ VITAMVISSIGGLILDTTVSDPNLVGIVVYTPVINGIGGNLVAIQASRISTYLHLHSIPG SEG PRD hcchhhhhhcceeeeccccccccceeeeeeceeeecccccceeeeehhhhhhhhhhcccc

MEM MMMMMMMMMMMMMMMM

SEQ ELPDEPKGCYYPFRTFFGPGVNNKSAQVLLLLVIPGHLIFLYTIHLMKΞGHTSLTI IFIV SEG PRD cccccccccccceeeeeccccchhhhhhhhhhccccchhhhhhhhcccccccceeeehhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMM...MMMMMMM

SEQ VYLFGAVLQVFTLLWIADWMVHHFWRKGKDPDSFSIPYLTALGDLLGTALLALSFHFLWL SEG xxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccceeeeeecchhhhhhhhhhhhheeee MEM MMMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMM

SEQ IGDRDGDVGD SEG PRD eecccccccc

MEM MM

Prosite for DKFZphtes3_27k4.1

PS00001 383->387 ASN_GLYCOSYLATION PDOC00001 PΞ00004 108->112 CAMP_PHOSPHO_SITE PDOC00004 PS00005 23->26 PKC_PHOSPHO_SITE PDOC00005 PS00005 65->68 PKC_PHOSPHO_SITE PDOC00005 PS00005 221->224 PKC_PHOSPHO_SITE PDOC00005 PS00006 5->9 CK2_PHOSPHO_SITE PDOC00006 PS00006 54->58 CK2_PHOSPHO_SITE PDOC00006 PS00006 146->150 CK2_PHOSPHO_SITE PDOC00006 PS00006 238->242 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 257->261 CK2_PHOSPHO_SITE PDOC00006 PS00006 296->300 CK2_PHOSPHO_SITE PDOC00006 PS00006 318->322 CK2_PHOΞPHO_SITE PDOC00006 PS00007 25->33 TYR_PHOSPHO_SITE PDOC00007 PS00008 90->96 MYRISTYL PDOC00008 PS00008 122-M28 MYRISTYL PDOC00008 PS00008 216->222 MYRISTYL PDOC00008 PS00008 220->226 MYRISTYL PDOC00008 PS00008 254->260 MYRISTYL PDOC00008

PS00008 336->342 MYRISTYL PDOC00008

PS00008 339->345 MYRISTYL PDOC00008

PS00013 12->23 PROKAR_LIPOPROTEIN PDOC00013

PS00013 248->259 PROKAR_LIPOPROTEIN PDOC00013

PS00029 459->481 LEUCINE ZIPPER PDOC00029

(No Pfam data available for DKFZphtes3_27k4.1)

DKFZphtes3_27ol4

group: testes derived

DKFZphtes3_27ol4 encodes a novel 358 am o acid protein with similarity to C. elegans cosmid C55A6.

The new protein contains a C3HC4 zmc finger (RING finger) signature. The ring fmger structure binds two atoms of zinc, and is involved in mediating protem-protem interactions. No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application m studying the expression profile of testis-specific genes . similarity to C. elegans C55A6.1 complete cDNA, complete eds, EST hits

Sequenced by GBF

Locus: /map="6"

Insert length: 2158 bp

Poly A stretch at pos. 2137, polyadenylation signal at pos. 2120

1 CCGAGGCCAG AGAGAAAAGA CTGCGAGGTG GCCGCAGCTG TGGCCGGAGA

51 GCACAAAGAA TGAACCAGCA GTGGAAGAGA AAATACTGTA AGCTGGCTGA

101 CTGCTGGTGA AGAAAATGCT TTATTTTTGT GGCAGGCATC TGTGGGATCT

151 GTAATAGAAA TATATTGGAG TAATTCAAGA TTCTGTGGTT GGCCCTTTTG

201 ACTGCTCTCT CTACAGGTTT AATTTGGGCA TTTACTCATT TTCATGGCTC

251 CAAGGACCAT GTATGTGTTG GGGATCTTCA ATATTCATGT TATTTTCTCC

301 TTTGGTCTTA TATGATTGTT ACCTTTATGA AGCTTTAGTG ATTACAAAGC

351 ACTTTTTTTG TCCATTTTTA CCTGAGCTTT GTAAACTCTG ATTTGCAGGA

401 TGGCTGGCTG TGGTGAAATT GATCATTCAA TAAACATGCT TCCTACAAAC

451 AGGAAAGCGA ACGAGTCCTG TTCTAATACT GCACCTTCTT TAACCGTCCC

501 TGAATGTGCC ATTTGTCTGC AAACATGTGT TCATCCAGTC AGTCTGCCCT

551 GTAAGCACGT TTTCTGCTAT CTATGTGTAA AAGGAGCTTC ATGGCTTGGA

601 AAGCGGTGTG CTCTTTGTCG ACAAGAAATT CCCGAGGATT TCCTTGACAA

651 GCCAACCTTG TTGTCACCAG AAGAACTCAA GGCAGCAAGT AGAGGAAATG

701 GTGAATATGC ATGGTATTAT GAAGGAAGAA ATGGGTGGTG GCAGTACGAT

751 GAGCGCACTA GTAGAGAGCT GGAAGATGCT TTTTCCAAAG GTAAAAAGAA

801 CACTGAAATG TTAATTGCTG GCTTTCTGTA TGTCGCTGAT CTTGAAAACA

851 TGGTTCAATA TAGGAGAAAT GAACATGGAC GTCGCAGGAA GATTAAGCGA

901 GATATAATAG ATATACCAAA GAAGGGAGTA GCTGGACTTA GGCTAGACTG

951 TGATGCTAAT ACCGTAAACC TAGCAAGAGA GAGCTCTGCT GACGGAGCGG

1001 ACAGTGTATC AGCACAGAGT GGAGCTTCTG TTCAGCCCCT AGTGTCTTCT

1051 GTAAGGCCCC TAACATCAGT AGATGGTCAG TTAACAAGCC CTGCAACACC

1101 ATCCCCTGAT GCAAGCACTT CTCTGGAAGA CTCTTTTGCT CATTTACAAC

1151 TCAGTGGAGA CAACACAGCT GAAAGGAGTC ATAGGGGAGA AGGAGAAGAA

1201 GATCATGAAT CACCATCTTC AGGCAGGGTA CCAGCACCAG ACACCTCCAT

1251 TGAAGAAACT GAATCAGATG CCAGTAGTGA TAGTGAGGAT GTATCTGCAG

1301 TTGTTGCACA GCACTCCTTG ACCCAACAGA GACTTTTGGT TTCTAATGCA

1351 AACCAGACAG TACCCGATCG ATCAGATCGA TCGGGAACTG ATCGATCAGT

1401 AGCAGGGGGT GGAACAGTGA GTGTCAGTGT CAGATCTAGA AGGCCTGATG

1451 GACAGTGCAC AGTAACTGAA GTTTAAATAA AAATGTCTTC AGCTCCATGC

1501 TCAAGGTTGA AAGGGTTACC TGTAAATTTC TGCCCACATA ACATTATACT

1551 CATCCCTAGT AGTGCATTTT GGGAGTTGGG GTGGGAAGGG GTATGGGAAG

1601 GATAGACTCA TAATTAAAAT GTCTAACATG TCTCTGTTGA GAAATTTATT

1651 TAATGTAAGG AACTTGGGTG TTAATAGTTG AGAGCTGTTT AGTAATAACC

1701 CAGTTTTCTT GAGGTCTGTT TACTTTATAC TTTTTAAAAA CTTCTGTAGT

1751 TCTTTTGGCC AGTGTGTTTG TATTATCTGT GCATTAATGG TCCTCATCTG

1801 ACTCCTGCAT TGTGTCTTAT TTTTCTGCAT GGATTGGCAT AAGACCATTA

1851 CTAAAATTTG GCACCTGTGA GATGTTTGAT ATTATGAACA GGAAACATAA

1901 TTTAATGTAT GAATAGATGT GAATTTGGGA TTTCAAAATA GATGAATAAC

1951 AACTATTTTA TAGTAAAGTT ATTGAAATGG AAATGAAAAC AGCCAGTAAC

2001 TTATGTTTCA GAATGTTTGT AACACACTTC ATGGTGTTCC CATAGGCTTT

2051 GCTGTCTAGT CTTATAGTTT GAGGTTTTTT TGGTCTGCAT TTTTCTTTTT

2101 GATTACAAAA TTTATAATTT AATAAATACT AGAGTTTATC AAAAAAAAAA

2151 AAAAAAAG

BLAST Results

Entry HSG117 from database EMBL: human STS SHGC-36270. Score = 1148, P = 8.9e-45, identities = 240/250 Medl e entries

No Medline entry

Peptide information for frame 1

ORF from 400 bp to 1473 bp; peptide length: 358 Category: similarity to unknown protein Prosite motifs: ZINC FINGER C3HC4 (51-61)

1 MAGCGEIDHS INMLPTNRKA NESCSNTAPS LTVPECAICL QTCVHPVSLP

51 CKHVFCYLCV KGASWLGKRC ALCRQEIPED FLDKPTLLSP EELKAASRGN

101 GEYAWYYEGR NGWWQYDERT ΞRELEDAFSK GKKNTEMLIA GFLYVADLEN

151 MVQYRRNEHG RRRKIKRDII DIPKKGVAGL RLDCDANTVN LARESSADGA

201 DSVSAQSGAS VQPLVSSVRP LTSVDGQLTS PATPSPDAST SLEDSFAHLQ

251 LSGDNTAERS HRGEGEEDHE SPSSGRVPAP DTSIEETESD ASSDSEDVSA

301 VVAQHSLTQQ RLLVSNANQT VPDRSDRSGT DRSVAGGGTV SVSVRSRRPD 351 GQCTVTEV

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_27ol4, frame 1

TREMBL :CEC55A6_1 gene: "C55A6.1"; Caenorhabditis elegans cosmid C55A6, N = 2, Score = 165, P = 4.2e-15

SWISSPROT :YWZ6_CAEEL HYPOTHETICAL 39.3 KD PROTEIN C02B8.6 IN CHROMOSOME X., N = 2, Score = 136, P = 3. le-11

>TREMBL:CEC55A6_1 gene: "C55A6.1"; Caenorhabditis elegans cosmid C55A6 Length = 484

HSPs:

Score = 165 (24.8 bits), Expect = 4.2e-15, Sum P(2) = 4.2e-15 Identities = 42/106 (39%), Positives = 61/106 (57%)

Query: 75 QEIPEDFLDKPTLLSPEELKAASRGNGEYAWYYEGRN-GWWQYDERTSRELEDAFSKGKK 133

Q +P LD ++ PEE K Y W Y G+N GWW+++ R RE+E+A++ GK Sbjct: 93 QNVPALDLDA-SICDPEERK Y-WIYSGKNQGWWRFEPRNEREIEEAYNAGKC 142

Query: 134 NTEMLIAGFLYVADLENMVQYRRNEHGRRRKIKR DIID-IPKKGVAGL 180

+ E++I G YV D +QY R + R +KR D D I KG+AG+ Sbjct: 143 HCEVVICGRPYVIDFHQFLQYPRGVPNQARHVKRVSADDFDGIGVKGLAGI 193

Score = 96 (14.4 bits), Expect = 4.2e-15, Sum P(2) = 4.2e-15 Identities = 19/54 (35%), Positives = 30/54 (55%)

Query: 35 ECAICLQTCVHPVSLP-CKHVFCYLCVKGAS —LGKRCALCRQEIPEDFLDKPT 86

EC IC + P ++P C H FC++C+KG +G C +CR I + +P+ Sbjct: 11 ECPICQCKMIVPTTIPACGHKFCFICLKGVYMNDMGG-CPMCRGPIDSNIFAQPS 64

Pedant information for DKFZphtes3_27ol4, frame 1

Report for DKFZphtes3_27ol4.1

[LENGTH] 358

[MW] 38818.90

[pi] 5.17

[HOMOL] TREMBL :CEC55A6_1 gene: "C55A6.1"; Caenorhabditis elegans cosmid C55A6 2e-12

[FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YCR066w] 3e-04

[FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YCR066w] 3e-04

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YCR066w] 3e-04 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation. palmitylation, farnesylation and processing) [S. cerevisiae, YCR066w] 3e-04

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YDR265w] 4e-04

[FUNCAT] 30.19 peroxisomal organization [S. cerevisiae, YDR265w] 4e-04

[BLOCKS] BL00518 Zmc finger, C3HC4 type, proteins

[PROSITE] MYRISTYL 2

[PROSITE] AMIDATION 3

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 12

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] ZINC_FINGER_C3HC4 1

[PROSITE] PKC_PHOSPHO_SITE 9

[PROSITE] ASN_GLYCOSYLATION 2

[PFAM] Zinc finger, C3HC4 type (RING finger)

[KW] Irregular

[KW] 3D

[KW] LOW COMPLEXITY 19.83 %

SEQ MAGCGEIDHSINMLPTNRKANESCSNTAPSLTVPECAICLQTCVHPVSLPCKHVFCYLCV SEG lrmd- TTTTTEETTTEEEETTTEEEEHHHH

SEQ KGASWLGKRCALCRQEIPEDFLDKPTLLSPEELKAASRGNGEYAWYYEGRNGWWQYDERT SEG lrmd- HHHHHHCCBTTTTTCBCGGG-CBCC

SEQ SRELEDAFSKGKKNTEMLIAGFLYVADLENMVQYRRNEHGRRRKIKRDIIDIPKKGVAGL SEG xxxxxxxxxxxxxxx lrmd-

SEQ RLDCDANTVNLARESSADGADSVSAQSGASVQPLVSSVRPLTSVDGQLTSPATPSPDAST SEG xxxxxxxxxxxx lrmd-

SEQ SLEDSFAHLQLSGDNTAERSHRGEGEEDHESPΞSGRVPAPDTSIEETESDASSDSEDVSA SEG X xxxxxxxxxxxxxxxxxxxx lrmd-

SEQ VVAQHSLTQQRLLVSNANQTVPDRSDRSGTDRSVAGGGTVSVSVRSRRPDGQCTVTEV SEG XXX xxxxxxxxxxxxxxxxxxxx lrmd-

Prosite for DKFZphtes3_27ol4.1

PS00001 21->25 ASN_GLYCOΞYLATION PDOC00001 PS00001 318->322 ASN_GLYCOSYLATION PDOC00001 PS00004 132->136 CAMP_PHOSPHO_SITE PDOC00004 PS00005 16->19 PKC_PHOSPHO_SITE PDOC00005 PS00005 120->123 PKC_PHOSPHO_SITE PDOC00005 PS00005 217->220 PKC_PHOSPHO_SITE PDOC00005 PS00005 260->263 PKC_PHOSPHO_SITE PDOC00005 PS00005 274->277 PKC_PHOSPHO_SITE PDOC00005 PS00005 325->328 PKC_PHOSPHO_SITE PDOC00005 PS00005 330->333 PKC_PHOSPHO_SITE PDOC00005 PS00005 343->346 PKC_PHOSPHO_SITE PDOC00005 PS00005 346->349 PKC_PHOSPHO_SITE PDOC00005 PS00006 32->36 CK2_PHOSPHO_SITE PDOC00006 PS00006 89->93 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 120->124 CK2_PHOSPHO_SITE PDOC00006 PS00006 195->199 CK2_PHOSPHO_SITE PDOC00006 PS00006 222->226 CK2_PHOSPHO_SITE PDOC00006 PS00006 240->244 CK2_PHOSPHO_SITE PDOC00006 PS00006 282->286 CK2_PHOSPHO_SITE PDOC00006 PS00006 287->291 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 293->297 CK2_PHOSPHO_SITE PDOC00006 PS00006 320->324 CK2_PHOSPHO_SITE PDOC00006 PS00006 328->332 CK2_PHOSPHO_SITE PDOC00006 PS00006 354->358 CK2_PHOSPHO_SITE PDOC00006 PS00007 98->107 TYR_PHOSPHO_SITE PDOC00007 PS00008 329->335 MYRISTYL PDOC00008 PΞ00008 337->343 MYRISTYL PDOC00008 PS00009 66->70 AMIDATION PDOC00009 PS00009 130->134 AMIDATION PDOC00009 PS00009 159->163 AMIDATION PDOC00009 PS00518 51->61 ZINC FINGER C3HC4 PDOC00449 Pfam for DKFZphtes3_27ol4.1

HMM_NAME Zmc finger, C3HC4 type (RING f ger)

HMM *CPICFcTFQlDyPWPFdePmMlPCgHsFCypCIrrW CPmC*

C+IC L + P++LPC+H+FCY C++ C +C Query 36 CAIC LQT CVHPVSLPCKHVFCYLCVKGASWLGKRCALC 73

DKFZphtes3_28dl4

group: testes derived

DKFZphtes3_28dl4 encodes a novel 97 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, EST hits

Sequenced by GBF

Locus: unknown

Insert length: 1279 bp

Poly A stretch at pos. 1232, no polyadenylation signal found

1 GGAGCTCAGA AGTTGGGCAA AGGTCACAGC AGACTTCCTG AAAAGCAGAC

51 ACTGAGGAAC ACAGTGGAGA GCGGGAGTTC ACAGCGACGC AGCTGAGGAC

101 GACGCAGGAC CTCTCCCAAA GGTGCTGCAG CTCCAGCACC AGGGGCCAGG

151 GCTGCGGCGA CAGCAGCTCA GCAACCCTTG CTGTGCTCAA GTTCTTGGGG

201 ATTCAGAGCT AAGTTCAAAA TTTAGAAACA GTGCCTTAAA GACGGGCAAG

251 AAAACCCGGT GTGGGAGTCT GCTCATCTAT GGTTTGTTAC TGCTCTCGCT

301 TTGATATTCT TAAATTCCTA GGTACCAATG AAAAAGCCAA GTGAACGTGG

351 CAGAGTGAGG AGGAGACAGG AGCGTGTGCA CCTTCCATCT GTGAGAGGCA

401 CACTTCAGTC TGGGTTCAAG ATGCAGAATG GTGCCTACAG CAAAAAAAAA

451 AAAAACACCC TCCTCCCTTC TTTACCATTT GAATGGACAT TTTCCTTACC

501 TGTGATCCCA ACAGAAACAG ATCCAGACCT ATCATGTGAA GTCCACGTTC

551 CAGGATCAGA AGTAACCAGT TTATGGACTG AGCTTACACG GGAAAGTCTA

601 CCCCCGACTC CTTCTGGATA GTAACATACA CAGCTGCATA AAAACGTCTC

651 CAAGGGGACA TACGATGCAT TTGCTTGGTG TCCCAGCCAA GCTCCCCACC

701 GGCGACCTCA CTGTTCCTTA GAGCTCGAGA GCTCGTCTCC TATCAATCAG

751 AGAACCCCAT CAGCTGTGAC CAACAGAGCT GGAGCCCTCT GTGGAGGGAG

801 CTGACCCCAC ACACAGGACA GAGCAGAATC CTGATTATTT TACAAACTGC

851 AAACCTTCTG AGTAAGAAGA CAAAAATATA CATTCCAAGG TATCTGTAAA

901 GTGCTTGGAA GATGCAGACA GCTGCACCGA GGGGCTCTGA TCCATCCACA

951 CGCTGCGCTT TGCTGCGGTC ACACACACGG TCTCAGTCAC GTGATGGTTT

1001 TGCTTTTATT TCTTAAACGG CTGAGTGATA ATCCAGCTAG TGTGCAGTCA

1051 TTTCATACCT TTCAATGGGC GTCACCGCAG TGACGCTGCC CCAGCCCCAT

1101 GCTGAGGGCC GACACAATTC ACGGAACAGA TTCATCATAT TTGGTCTTTA

1151 TGTAAATAAT AAATGTTTTA AAATTGCCTA AATATAAAAA AAAAAAAAAA

1201 AAAAAAAAAA AAAAAAAAAA AAAGGGCGGC CGAAAAAAAA AAAAAAAAAA

1251 AAAAAAAAAA AAAAAAAAAA GGGCGGCCG

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 1

ORF from 328 bp to 618 bp; peptide length: 97 Category: putative protein

1 MKKPSERGRV RRRQERVHLP SVRGTLQSGF KMQNGAYSKK KKNTLLPSLP 51 FEWTFSLPVI PTETDPDLSC EVHVPGSEVT SLWTELTRES LPPTPSG

BLASTP hits

No BLASTP hits available Alert BLASTP hits for DKFZphtes3_28dl4, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphtes3_28dl4, frame 1

Report for DKFZphtes3_28dl4.1

[LENGTH] 97

[MW] 10945.56

[pi] 9.80

[PROSITE] MYRISTYL 2

[PROSITE] CAMP_PHOSPHO_SITE 2

[PROSITE] CK2_PHOSPHO_SITE 2

[PROSITE] PKC_PHOSPHO_SITE 3

[KW] All_Alpha

[KW] LOW COMPLEXITY 12.37

SEQ MKKPSERGRVRRRQERVHLPSVRGTLQSGFKMQNGAYSKKKKNTLLPSLPFEWTFSLPVI SEG xxxxxxxxxxxx PRD cccccchhhhhhhhhhhccccccccccccccccccccccccccccccccccccccccccc

SEQ PTETDPDLSCEVHVPGSEVTSLWTELTRESLPPTPSG SEG PRD ccccccccceeeecccccchhhhhhhhhhcccccccc

Prosite for DKFZphtes3_28dl4.1

PS00004 2->6 CAMP_PHOSPHO_SITE PDOC00004

PS00004 41->45 CAMP_PHOSPHO_SITE PDOC00004

PS00005 5->8 PKC_PHOSPHO_SITE PDOC00005

PS00005 21->24 PKC_PHOSPHO_SITE PDOC00005

PS00005 38->41 PKC_PHOSPHO_SITE PDOC00005

PS00006 62->66 CK2_PHOSPHO_SITE PDOC00006

PS00006 64->68 CK2_PHOSPHO_SITE PDOC00006

PS00008 24->30 MYRISTYL PDOC00008

PS00008 76->82 MYRISTYL PDOC00008

(No Pfam data available for DKFZphtes3_28dl4.1)

DKFZphtes3_2all

group: testes derived

DKFZphtes3_2all encodes a novel 1048 amino acid protein with very weak similarity to mucins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application m studying the expression profile of testis-specific genes . similarity to mucin complete cDNA, complete eds, EST hits

Sequenced by EMBL

Locus: unknown

Insert length: 4082 bp

Poly A stretch at pos. 4060, polyadenylation signal at pos. 4034

1 GAGGACTGCG AGCACAGCGG CGGCCGGGTG GCGGGGGTGA GTGGGGCCAG

51 CGGGGCTGGA CAGCAGCGGG CCCCGGGCGC CGCCGCCGCG ATCCCTCCCC

101 GCGCCCGCCG AGCACATCGC CGCCGCCGAG ATGGGCCCTC CGCGGCACCC

151 CCAGGCCGGC GAGATAGAAG CGGGCGGTGC GGGCGGCGGG CGGCGGCTAC

201 AGGTGGAAAT GAGTTCTCAA CAGTTTCCTC GGTTAGGAGC CCCTTCTACC

251 GGGCTGAGCC AGGCCCCTTC TCAGATTGCA AACAGTGGTT CTGCTGGATT

301 GATAAACCCA GCTGCTACAG TCAATGATGA ATCTGGTCGA GATTCTGAAG

351 TCAGTGCCAG GGAGCACATG AGTTCCAGCA GCTCCCTCCA GTCCCGGGAG

401 GAGAAGCAAG AGCCTGTTGT GGTAAGGCCC TATCCACAGG TGCAGATGTT

451 GTCGACACAC CATGCTGTCG CATCAGCCAC ACCTGTTGCA GTGACAGCCC

501 CGCCAGCACA CCTGACGCCA GCAGTGCCAC TTTCATTTTC GGAGGGACTT

551 ATGAAGCCGC CCCCGAAGCC CACCATGCCT AGCCGTCCCA TTGCTCCTGC

601 TCCACCTTCT ACCCTGTCAC TTCCCCCCAA GGTTCCAGGG CAGGTTACCG

651 TTACCATGGA GAGTAGCATC CCTCAAGCTT CAGCCATTCC TGTGGCAACA

701 ATCAGTGGAC AACAGGGCCA TCCCAGTAAC CTGCATCACA TCATGACTAC

751 AAATGTGCAA ATGTCTATCA TCCGCAGCAA TGCTCCTGGG CCCCCTCTTC

801 ACATTGGAGC TTCTCATTTA CCTCGAGGTG CAGCTGCTGC TGCTGTGATG

851 TCCAGTTCTA AAGTAACCAC AGTCCTGAGG CCGACCTCAC AGCTGCCAAA

901 TGCTGCTACT GCTCAGCCAG CAGTACAGCA CATCATTCAC CAACCAATCC

951 AGTCTCGGCC ACCTGTGACC ACCTCCAATG CCATCCCTCC TGCTGTGGTA

1001 GCAACTGTCT CAGCCACCAG AGCTCAGTCT CCAGTCATCA CTACGACAGC

1051 GGCGCATGCT ACTGATTCAG CACTTAGTAG GCCAACCTTG TCTATCCAGC

1101 ATCCTCCATC TGCAGCAATC AGTATTCAGC GTCCTGCCCA GTCACGAGAT

1151 GTCACAACAA GAATCACACT ACCATCTCAC CCTGCATTAG GGACGCCAAA

1201 ACAGCAGCTT CATACAATGG CTCAGAAAAC AATCTTCAGT ACTGGCACGC

1251 CAGTGGCTGC AGCCACAGTA GCACCTATTT TGGCAACCAA CACCATTCCT

1301 TCAGCGACCA CAGCTGGATC TGTGTCACAC ACGCAAGCTC CCACAAGTAC

1351 CATTGTTACC ATGACAGTAC CCTCCCATTC CTCCCATGCT ACTGCTGTGA

1401 CCACCTCAAA CATCCCAGTC GCCAAGGTGG TGCCCCAGCA GATCACGCAC

1451 ACTTCTCCTC GGATCCAGCC AGACTACCCT GCCGAGAGGA GTAGCCTGAT

1501 TCCCATCTCC GGACATCGGG CCTCTCCCAA TCCTGTGGCC ATGGAAACCC

1551 GAAGTGACAA CAGACCGTCT GTTCCCGTTC AGTTCCAATA TTTTTTGCCA

1601 ACTTACCCCC CTTCTGCATA CCCACTGGCG GCACATACCT ACACCCCAAT

1651 CACCAGTTCC GTGTCCACTA TCCGACAGTA TCCAGTTTCA GCTCAGGCTC

1701 CAAACTCTGC CATCACAGCT CAGACTGGTG TTGGGGTAGC GTCTACCGTC

1751 CACCTAAACC CCATGCAGTT GATGACAGTG GATGCATCGC ATGCTCGACA

1801 TATTCAAGGG ATCCAGCCAG CACCCATCAG TACCCAGGGT ATCCAGCCGG

1851 CCCCCATTGG GACCCCAGGG ATACAGCCTG CACCACTTGG CACACAGGGA

1901 ATTCACTCAG CAACCCCAAT CAACACACAA GGGCTTCAGC CTGCACCTAT

1951 GGGTACTCAG CAGCCTCAGC CTGAAGGAAA GACTTCAGCA GTGGTGTTGG

2001 CAGATGGAGC CACAATTGTG GCCAACCCTA TTAGCAATCC ATTCAGTGCT

2051 GCTCCAGCAG CAACAACCGT GGTGCAGACC CACAGCCAGA GTGCTAGCAC

2101 CAACGCTCCC GCCCAGGGCT CATCGCCACG GCCAAGCATA CTCCGGAAGA

2151 AACCTGCCAC AGATGGTGCC AAACCCAAGT CTGAAATCCA CGTGTCTATG

2201 GCCACTCCGG TCACTGTGTC CATGGAGACT GTATCCAATC AAAATAATGA

2251 TCAGCCTACC ATTGCCGTCC CTCCAACTGC CCAGCAGCCC CCACCGACCA

2301 TTCCAACTAT GATTGCAGCA GCCAGTCCCC CGTCACAACC AGCCGTTGCC

2351 CTTTCAACCA TTCCTGGAGC GGTCCCCATC ACTCCACCCA TCACCACCAT

2401 TGCAGCTGCA CCACCTCCAT CAGTCACTGT GGGTGGCAGT CTTTCCTCCG

2451 TCTTGGGCCC TCCCGTTCCT GAAATTAAAG TGAAAGAAGA AGTAGAACCA

2501 ATGGATATCA TGAGGCCAGT TTCTGCAGTT CCTCCACTGG CTACCAACAC

2551 TGTGTCTCCA TCTCTTGCAT TGCTGGCAAA CAACTTGTCC ATGCCTACAA

2601 GTGACCTACC ACCTGGTGCC TCCCCAAGGA AAAAGCCTCG AAAGCAACAG

2651 CATGTGATCT CAACAGAAGA AGGTGACATG ATGGAGACAA ACAGCACTGA

2701 TGATGAGAAG TCCACTGCCA AGAGTCTTCT GGTGAAGGCT GAGAAGCGCA 2751 AGTCTCCTCC CAAGGAGTAT ATTGATGAGG AAGGTGTGAG ATATGTCCCA

2801 GTGCGTCCAA GACCCCCCAT TACTTTGCTT CGTCACTATC GGAACCCCTG

2851 GAAAGCTGCT TACCACCACT TTCAGAGGTA CAGTGACGTC CGGGTCAAAG

2901 AGGAGAAGAA AGCTATGCTG CAGGAAATAG CTAATCAGAA AGGAGTATCC

2951 TGTCGTGCTC AAGGCTGGAA AGTCCACCTC TGTGCTGCCC AGTTACTACA

3001 GCTGACGAAT CTAGAACATG ATGTCTATGA AAGACTTACT AACCTGCAGG

3051 AAGGGATTAT CCCAAAGAAA AAAGCAGCAA CAGATGATGA TCTCCACCGA

3101 ATAAACGAAC TGATACAGGG AAATATGCAG AGGTGTAAAC TTGTGATGGA

3151 TCAAATCAGT GAAGCCAGAG ACTCCATGCT TAAGGTTTTA GATCATAAAG

3201 ACCGTGTCCT GAAGCTGCTT AACAAGAACG GGACTGTCAA AAAAGTGTCC

3251 AAATTGAAGC GAAAGGAAAA AGTCTAGACC CAGAACAATC AGGAGATTGG

3301 AAGCAAATTT ATGAAGAATG ATGGTGGGGG TGGGGGGAGG GTTTTGGTTT

3351 TTTCCAAAGT GGAACATTGA AATAAAGGAA GTGTTCCTTA GTTCCCGTGT

3401 GAAAGCAGAG GAACCCATGA CATCCAAGGG CGTGAAAGGA TCAGAGCTGA

3451 CTGGACATAG TGAGCTGCCT TCTTGCGTTC GGGTGCACCC CTGTTAAACC

3501 TGATCTGTGT CATAAGTGAC TCCGGATGCA TCAGTGTCCA CCAGTTGGAA

3551 GCAATGACAA GGATGGCTGG CTGGTGTTTT TCAGCCTTCC GGTTTATAGA

3601 CTGTATTTAT CTAGTGGATT CCTGCAGGCC CCATACTGAG CCTGGACTGA

3651 AAGTATCCAC TCGGACCATC TGTTATCTCT CTACACTGAA AATAAAACCT

3701 CTTCCACCCA CCCCATTCGG TTCTTCTGCC TGACCTTCAA ATGCCCATGT

3751 TGGCCTTTTA CAGCAGTGCC ACGGCACCAA GCGAGCTGCC ACATCTCACA

3801 CTCTAAAGGG TTTGAACTAT TAGTTCTTGT CATTTTTTAA AAAAAACCAT

3851 TCCCAAGTGA AATTGTTATA TCGTCTGTCT TGCGTGTGTC AGAACTGGGT

3901 TTTTGTGGAG GTTCAGAGCA GGCAACACCA TAAGTTGCTC TCAGATCCTT

3951 GTTCTGAAGT ACATTCTTGG TTATCTGTAC TTCTGTAGCT GGTGTGATGC

4001 TGTTAATTGT ATGTACCACA CATCTCCAGA CGTTAATAAA GGACTCAAAG

4051 AGGTTTTTGT AAAAAAAAAA AAAAAAAAAA AA

BLAST Results

No BLAST result

Medline entries

No Medl e entry

Peptide information for frame 2

ORF from 131 bp to 3274 bp; peptide length: 1048 Category: similarity to known protein

1 MGPPRHPQAG EIEAGGAGGG RRLQVEMSSQ QFPRLGAPST GLSQAPSQIA

51 NSGSAGLINP AATVNDESGR DSEVΞAREHM SSSSSLQSRE EKQEPVVVRP

101 YPQVQMLSTH HAVASATPVA VTAPPAHLTP AVPLSFSEGL MKPPPKPTMP

151 SRPIAPAPPS TLSLPPKVPG QVTVTMESSI PQASAIPVAT ISGQQGHPSN

201 LHHIMTTNVQ MSIIRSNAPG PPLHIGASHL PRGAAAAAVM SSΞKVTTVLR

251 PTSQLPNAAT AQPAVQHIIH QPIQSRPPVT TSNAIPPAVV ATVSATRAQS

301 PVITTTAAHA TDSALSRPTL SIQHPPSAAI SIQRPAQSRD VTTRITLPSH

351 PALGTPKQQL HTMAQKTIFS TGTPVAAATV APILATNTIP SATTAGSVSH

401 TQAPTSTIVT MTVPSHSΞHA TAVTTSNIPV AKVVPQQITH TSPRIQPDYP

451 AERSSLIPIΞ GHRASPNPVA METRSDNRPS VPVQFQYFLP TYPPSAYPLA

501 AHTYTPITSS VSTIRQYPVS AQAPNSAITA QTGVGVASTV HLNPMQLMTV

551 DASHARHIQG IQPAPISTQG IQPAPIGTPG IQPAPLGTQG IHSATPINTQ

601 GLQPAPMGTQ QPQPEGKTSA VVLADGATIV ANPISNPFSA APAATTVVQT

651 HSQSASTNAP AQGSSPRPSI LRKKPATDGA KPKSEIHVSM ATPVTVSMET

701 VSNQNNDQPT IAVPPTAQQP PPTIPTMIAA ASPPSQPAVA LSTIPGAVPI

751 TPPITTIAAA PPPSVTVGGS LSSVLGPPVP EIKVKEEVEP MDIMRPVSAV

801 PPLATNTVSP SLALLANNLS MPTSDLPPGA SPRKKPRKQQ HVISTEEGDM

851 METNSTDDEK STAKSLLVKA EKRKSPPKEY IDEEGVRYVP VRPRPPITLL

901 RHYRNPWKAA YHHFQRYSDV RVKEEKKAML QEIANQKGVS CRAQGWKVHL

951 CAAQLLQLTN LEHDVYERLT NLQEGIIPKK KAATDDDLHR INELIQGNMQ

1001 RCKLVMDQIS EARDSMLKVL DHKDRVLKLL NKNGTVKKVS KLKRKEKV

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_2all, frame 2

SWISSPROT :MUC2_HUMAN MUCIN 2 PRECURSOR (INTESTINAL MUCIN 2)., N = 1, Score = 334, P = 2.4e-25 PIR:A43932 mucin 2 precursor, intestinal - human (fragments), N = 1, Score = 321, P = 3.2e-24

TREMBL :D88440_1 product: "high molecular mass nuclear antigen"; Gallus gallus mRNA for high molecular mass nuclear antigen, partial eds., N = 1, Score = 312, P = 8.3e-24

PIR:S48478 glucan 1, 4-alpha-glucosιdase (EC 3.2.1.3) - yeast (Saccharomyces cerevisiae), N = 1, Score = 300, P = 2.1e-22

>SWISSPROT:MUC2_HUMAN MUCIN 2 PRECURSOR (INTESTINAL MUCIN 2) . Length = 5,179

HSPs:

Score = 334 (50.1 bits), Expect = 2.4e-25, P = 2.4e-25 Identities = 184/770 (23%), Positives = 263/770 (34%)

Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154

V P P T + + T V T P TP + + P P PT P Sbjct: 3471 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 3530

Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212

P +T P P G T T + P T +G Q P+ TT V + Sbjct: 3531 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 3589

Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268

+ P P+ + P +++ +TT T T P I

Sbjct: 3590 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 3649

Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328

+ P T P T + T +P T T T + T++ P Sbjct: 3650 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 3706

Query: 329 AISIQRPAQΞRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385

Q P + TT P+ GT + T + T TP T PI Sbjct: 3707 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3766

Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443

T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 3767 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 3825

Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502

+ P ++ + +P P +T + + P+ + PT P+ Sbjct: 3826 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 3874

Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560

T TPIT++ + T P Q P + IT T V T Q T Sbjct: 3875 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 3932

Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613

P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 3933 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 3991

Query: 614 -PEGKTΞAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSIL 671

P T+ V T P + P + T T +Q+ +T ++ P+ Sbjct: 3992 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 4051

Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728

T P + TP +T + T P PT Q P T P Sbjct: 4052 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 4111

Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPΞ VTVGGSLSSVLGP-PVPEI 782 p+ T P PIT ττ+ P P+ T + ++ + p p p

Sbjct: 4112 TTTVTPTPTPTGTQT-PTTTPITTT-TTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTG 4169

Query: 783 KVKEEVEPMDIMRPVSAVP-PLATNTVSPSLALLANNLSMPTSDLPPGASPRKKPRKQQH 841

P+ V+ P P T T P+ A + TS+ PP +S + R Sbjct: 4170 TQTPTTTPITTTTTVTPTPTPTGTQTGPPTHTΞTAPIAELTTSNPPPESSTPQTSRSTSΞ 4229

Query: 842 VISTEEGDMMET 853

+ TE ++ T Sbjct: 4230 PL-TESTTLLST 4240

Score = 328 (49.2 bits), Expect = 1.0e-24, P = 1.0e-24 Identities = 180/745 (24%), Positives = 254/745 (34%)

Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154

V P P T + + T V T P TP + + P P PT P Sbjct: 3540 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 3599 Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212

P +T P P G T T + P T +G Q P+ TT V + Sbjct: 3600 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 3658

Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268

+ P P+ + P +++ +TT T T P I

Sbjct: 3659 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 3718

Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328

+ P T P T + T +P T T T + T++ P Sbjct: 3719 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 3775

Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385

Q P + TT P+ GT + T + T TP T PI Sbjct: 3776 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3835

Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443

T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 3836 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 3894

Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502

+ P ++ + +P P +T + + P+ + PT P+ Sbjct: 3895 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 3943

Query: 503 TYTPITΞSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560

T TPIT++ + T P Q P + IT T V T Q T Sbjct: 3944 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 4001

Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613

P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 4002 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 4060

Query: 614 -PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSIL 671

P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 4061 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 4120

Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728

T P + TP +T + T P PT Q P T P Sbjct: 4121 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 4180

Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAA-PPPSVTVGGSLSSVLGPPVPEIKVKEE 787

P+ T P T PI + + PPP + + S P + Sbjct: 4181 TTTVTPTPTPTGTQTGPPTHTSTAPIAELTTSNPPPESSTPQTSRSTSSPLTESTTLLST 4240

Query: 788 VEPMDIMRPVSAVPPLATNTVΞPSLALLANNLSMP—TSDLPPGASPR 833

+ P M S PP +T T +P+ + LS P T+ PPG R Sbjct: 4241 LPPAIEM—TSTAPP-STPT-APTTTSGGHTLSPPPSTTTSPPGTPTR 4284

Score = 325 (48.8 bits), Expect = 2.2e-24, P = 2.2e-24 Identities = 186/782 (23%), Positives = 261/782 (33%)

Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPΞRPI 154

V P P T + + T V T P TP + + P P PT P Sbjct: 3494 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 3553

Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212

P +T P P G T T + P T +G Q P+ TT V + Sbjct: 3554 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 3612

Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268

+ P P+ + P +++ +TT T T P I

Sbjct: 3613 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 3672

Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVΞATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328

+ P T P T + T +P T T T + T++ P Sbjct: 3673 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 3729

Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385

Q P + TT P+ GT + T + T TP T PI Sbjct: 3730 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3789

Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443

T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 3790 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 3848

Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502

+ P ++ + +P P +T + + P+ + PT P+ Sbjct: 3849 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 3897

Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560 T TPIT++ + T P Q P + IT T V T Q T Sbjct: 3898 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 3955

Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613

P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 3956 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 4014

Query: 614 -PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSΞPRPSIL 671

P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 4015 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 4074

Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728

T P + TP +T + P PT Q P T P Sbjct: 4075 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 4134

Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788

P+ T P PIT TT P P+ T G+ + P I V Sbjct: 4135 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQT PTTTPITTTTTV 4184

Query: 789 EPMDIMRPVSAVPPLATNTVSPSLALLANNLSMPTSDLPPGASPRKKPRKQQHVISTEEG 848

P PP T+T +P L +N P S P + P + + +

Sbjct: 4185 TPTPTPTGTQTGPPTHTST-APIAELTTSN-PPPESSTPQTSRSTSSPLTESTTLLSTLP 4242

Query: 849 DMMETNSTDDEKSTAKSLLVKAEKRKSPP 877

+E ST + SPP

Sbjct: 4243 PAIEMTSTAPPSTPTAPTTTSGGHTLSPP 4271

Score = 324 (48.6 bits), Expect = 2.8e-24, P = 2.8e-24 Identities = 170/717 (23%), Positives = 248/717 (34%)

Query: 95 PVVVRPYPQVQMLSTHHAVASATP—VAVTAPPAHLTPAVPLSFSEGLMKPPPKPTMPSR 152

P P P +T + +P T PP TP+ P++ + + P P+ P Sbjct: 1401 PPTTTPSPPPTTTTTLPPTTTPSPPTTTTTTPPPTTTPSPPITTTTTPL-PTTTPSPPIS 1459

Query: 153 PIAPAPPSTLSLPPKVPGQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212

PP+T PP T S + P T + P I + Sbjct: 1460 TTTTPPPTTTPSPPTTTPSPPTTTPSPPTTTTTTPPPTTTPS PPMTTPITPPASTTT 1516

Query: 213 IIRSNAPGPPLHIGASHLPRGAAAAAVMSSSKVTTVLRPTSQ—LPNAATAQPAVQHIIH 270

+ + P PP + P S T + PTS LP T P

Sbjct: 1517 LPPTTTPSPPTTTTTTPPP TTTPSPPTTTPITPPTSTTTLPPTTTPSPPPTTTTT 1571

Query: 271 QPIQSRP-PVTTSNAIPPAVVATVSA-TRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328

P + P P TT+ PP + T T SP TTT + S PT + PP++ Sbjct: 1572 PPPTTTPSPPTTTTPSPPTITTTTPPPTTTPSPPTTTTTTPPPTTTPSPPTTTPITPPTS 1631

Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKTIFSTGTPVAAATVAPILATNT 388

++ T T P P TP T I +T TP T + + T

Sbjct: 1632 TTTLPPTTTPSPPPTTTTTP—PPTTTPSPPTTTTPSPPITTTTTPPPTTTPSSPITTTP 1689

Query: 389 IPSATTAGSVSHTQAPTSTIVTMTVPSHSSHATAV-TTSNIPVAKVVPQQITHTSPRIQP 447

P TT + S T P+S I T T PS ++ + TT P P T T + P Sbjct: 1690 SPPTTTMTTPSPTTTPSSPITTTTTPΞSTTTPSPPPTTMTTPSPTTTPSPPTTTMTTLPP 1749

Query: 448 DYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPV-QFQYFLPTYPPSAY-P LA 500

+ + P+ P T + P VP+ + +L + P+ + P L Sbjct: 1750 TTTSSPLTTTPLPPSITPPTFSPFSTTTPTTPCVPLCNWTGWLDSGKPNFHKPGGDTELI 1809

Query: 501 AHTYTPITSΞVSTIR—QYP-VSAQAPNSAITAQTGVG-VASTVHLNPMQLMTVDASHAR 556

P ++ + R YP V + VG + P ++ + A Sbjct: 1810 GDVCGPGWAANISCRATMYPDVPIGQLGQTVVCDVSVGLICKNEDQKPGGVIPM-AFCLN 1868

Query: 557 HIQGIQPAPISTQGIQPAPIGTPGIQ-PAPLGTQGIHSATPINTQGLQPAPMGTQQPQ— 613

+ +Q TQ P + T + P P T I + T + P P GTQ P Sbjct: 1869 YEINVQCCECVTQ PTTMTTTTTENPTPPTTTPITTTTTVTPT PTPTGTQTPTTT 1922

Query: 614 PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSILR 672

P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 1923 PITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP 1982

Query: 673 KKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMIA 729

T P + TP +T + T P PT Q P T P Sbjct: 1983 TGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTT 2042

Query: 730 AASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEVE 789

P+ T P PIT TT P P+ T G+ + P V Sbjct: 2043 TTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTPT 2096

Query: 790 PMDIMRPVSAVPPLATNTVSPS 811

P P + P T TV+P+ Sbjct: 2097 PTGTQTPTTT-PITTTTTVTPT 2117 Score = 318 (47.7 bits), Expect = 1.2e-23, P = 1.2e-23 Identities = 174/717 (24%), Positives = 243/717 (33%)

Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154

V P P T + + T V T P TP + + P P PT P Sb_jct: 2068 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 2127

Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212

P +T P P G T T + P T +G Q P+ TT V + Sbjct: 2128 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 2186

Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268

+ P P+ + P +++ +TT T T P I

Sbjct: 2187 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 2246

Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328

+ P T P T + T +P T T T + T++ P Sbjct: 2247 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 2303

Query: 329 AISIQRPAQΞRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385

Q P + TT P+ GT + T + T TP T PI Sbjct: 2304 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2363

Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTΞNIPVAKVVPQQITHTSP 443

T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 2364 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 2422

Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502

+ P ++ + +P P +T + + P+ + PT P+ Sbjct: 2423 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 2471

Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560

T TPIT++ + T P Q P + IT T V T Q T Sbjct: 2472 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 2529

Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613

P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 2530 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 2588

Query: 614 -PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSΞPRPSIL 671

P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 2589 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 2648

Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728

T P + TP +T + T P PT Q P T P Sbjct: 2649 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2708

Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788

P+ T P PIT TT P P+ T G+ + P V Sbjct: 2709 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTP 2762

Query: 789 EPMDIMRPVSAVPPLATNTVSPS 811

P P + P T TV+P+ Sbjct: 2763 TPTGTQTPTTT-PITTTTTVTPT 2784

Score = 318 (47.7 bits), Expect = 1.2e-23, P = 1.2e-23 Identities = 174/717 (24%), Positives = 243/717 (33%)

Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154

V P P T + + T V T P TP + + P P PT P Sbjct: 2206 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 2265

Query: 155 A-PAPPSTLΞLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212

P +T P P G T T + P T +G Q P+ TT V + Sbjct: 2266 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 2324

Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268

+ P P+ + P +++ +TT T T P I

Sbjct: 2325 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 2384

Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328

+ P T P T + T +P T T T + T++ P Sbjct: 2385 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 2441

Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385

Q P + TT P+ GT + T + T TP T PI Sb_jct: 2442 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2501

Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSΞHATAVTTSNIPVAKVVPQQITHTSP 443 T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 2502 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 2560 Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502

+ p ++ + +p p +τ + + p+ + PT p+

Sbjct: 2561 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 2609

Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560

T TPIT++ + T P Q P + IT T V T Q T Sbjct: 2610 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 2667

Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613

P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 2668 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 2726

Query: 614 -PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSΞPRPSIL 671

P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 2727 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 2786

Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728

T P + TP +T + T P PT Q P T P Sbjct: 2787 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2846

Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788

P+ T P PIT TT P P+ T G+ + P V Sbjct: 2847 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTP 2900

Query: 789 EPMDIMRPVSAVPPLATNTVSPS 811

P P + P T TV+P+ Sbjct: 2901 TPTGTQTPTTT-PITTTTTVTPT 2922

Score = 318 (47.7 bits). Expect = 1.2e-23, P = 1.2e-23 Identities = 174/717 (24%), Positives = 243/717 (33%)

Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154

V P P T + + T V T P TP + + P P PT P Sbjct: 2321 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 2380

Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212

P +T P P G T T + P T +G Q P+ TT V + Sbjct: 2381 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 2439

Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268

+ P P+ + P +++ +TT T T P I

Sbjct: 2440 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 2499

Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328

+ P T P T + T +P T T T + T++ P Sbjct: 2500 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 2556

Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385

Q P + TT P+ GT + T + T TP T PI Sbjct: 2557 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2616

Query: 386 TNTI-PSATTAGΞVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443

T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 2617 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 2675

Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502

+ P ++ + +P P +T + + P+ + PT P+ Sbjct: 2676 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 2724

Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560

T TPIT++ + T P Q P + IT T V T Q T Sbjct: 2725 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 2782

Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613

P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 2783 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 2841

Query: 614 -PEGKTSAVVLADGATIVANPISNPFΞAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSIL 671

P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 2842 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 2901

Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728

T P + TP +T + T P PT Q P T P Sbjct: 2902 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2961

Query: 729 AAASPPSQPAVALΞTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788

P+ T P PIT TT P P+ T G+ + P V Sbjct: 2962 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTP 3015 Query: 789 EPMDIMRPVSAVPPLATNTVSPS 811

P P + P T TV+P+ Sb_jct: 3016 TPTGTQTPTTT-PITTTTTVTPT 3037

Score = 318 (47.7 bits). Expect = 1.2e-23, P = 1.2e-23 Identities = 174/717 (24%), Positives = 243/717 (33%)

Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154

V P P T + + T V T P TP + + P P PT P Sbjct: 2390 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 2449

Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212

P +T P P G T T + P T +G Q P+ TT V + Sbjct: 2450 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 2508

Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268

+ P P+ + P +++ +TT T T P I

Sbjct: 2509 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 2568

Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALΞRPTLSIQHPPSA 328

+ P T P T + T +P T T T + T++ P Sbjct: 2569 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 2625

Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385

Q P + TT P+ GT + T + T TP T PI Sbjct: 2626 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2685

Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443

T τ+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 2686 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 2744

Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502

+ p ++ + +p p +τ + + p+ + PT p+

Sbjct: 2745 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 2793

Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560

T TPIT++ + T P Q P + IT T V T Q T Sbjct: 2794 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 2851

Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- "613

P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 2852 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 2910

Query: 614 -PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSIL 671

P T+ V T P + P + T T +Q+ +T ++ P+ Sbjct: 2911 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 2970

Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728

T P + TP +T + T P PT Q P T P Sbjct: 2971 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3030

Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788

P+ T P PIT TT P P+ T G+ + P V Sbjct: 3031 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTP 3084

Query: 789 EPMDIMRPVSAVPPLATNTVSPS 811

P P + P T TV+P+ Sbjct: 3085 TPTGTQTPTTT-PITTTTTVTPT 3106

Score = 318 (47.7 bits), Expect = 1.2e-23, P = 1.2e-23 Identities = 174/717 (24%), Positives = 243/717 (33%)

Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154

V P P T + + T V T P TP + + P P PT P Sbjct: 2459 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 2518

Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212

P +T P P G T T + P T +G Q P+ TT V + Sbjct: 2519 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 2577

Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268

+ P P+ + P +++ +TT T T P I

Sbjct: 2578 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 2637

Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328

+ P T P T + T +P T T T + T++ P Sbjct: 2638 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 2694

Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385

Q P + TT P+ GT + T + T TP T PI Sbjct: 2695 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2754 Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAK VPQQITHTSP 443

T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 2755 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 2813

Query: 444 RIQPDYPAERSΞLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502

+ p ++ + +p p +τ + + p+ + PT p+

Sbjct: 2814 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG--TQTP 2862

Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560

T TPIT++ + T P Q P + IT T V T Q T Sbjct: 2863 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 2920

Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613

P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 2921 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 2979

Query: 614 -PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSIL 671

P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 2980 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 3039

Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728

T P + TP +T + T P PT Q P T P Sbjct: 3040 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3099

Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788

P+ T P PIT TT P P+ T G+ + P V Sbjct: 3100 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTP 3153

Query: 789 EPMDIMRPVSAVPPLATNTVSPS 811

P P + P T TV+P+ Sbjct: 3154 TPTGTQTPTTT-PITTTTTVTPT 3175

Score = 318 (47.7 bits), Expect = 1.2e-23, P = 1.2e-23 Identities = 174/717 (24%), Positives = 243/717 (33%)

Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154

V P P T + + T V T P TP + + P P PT P Sbjct: 2528 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 2587

Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212

P +T P P G T T + P T +G Q P+ TT V + Sbjct: 2588 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 2646

Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268

+ P P+ + P +++ +TT T T P I

Sbjct: 2647 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 2706

Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328

+ P T P T + T +P T T T + T++ P Sbjct: 2707 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 2763

Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385

Q P + TT P+ GT + T + T TP T PI Sbjct: 2764 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2823

Query: 386 TNTI-PSATTAGSVSHTQAPTΞTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443

T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 2824 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 2882

Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502

+ P ++ + +P P +T + + P+ + PT P+ Sbjct: 2883 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 2931

Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560

T TPIT++ + T P Q P + IT T V T Q T Sbjct: 2932 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 2989

Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613

P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 2990 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 3048

Query: 614 -PEGKTSAVVLADGATIVANPIΞNPFSAAPAAT-TVVQTHSQΞASTNAPAQGSSPRPSIL 671

P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 3049 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 3108

Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728

T P + TP +T + T P PT Q P T P Sbjct: 3109 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3168

Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788 P+ T P PIT TT P P+ T G+ + P V Sbjct: 3169 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT--GTQTPTTTPITTTTTVTPTP 3222

Query: 789 EPMDIMRPVSAVPPLATNTVSPS 811

P P + P T TV+P+ Sbjct: 3223 TPTGTQTPTTT-PITTTTTVTPT 3244

Score = 318 (47.7 bits), Expect = 1.2e-23, P = 1.2e-23 Identities = 174/717 (24%), Positives = 243/717 (33%)

Query: 96 VVVRPYPQVQMLSTHHAVAΞATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPΞRPI 154

V P P T + + T V T P TP + + P P PT P Sbjct: 3080 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 3139

Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212

P +T P P G T T + P T +G Q P+ TT V + Sbjct: 3140 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 3198

Query: 213 IIRSNAPGP PLHIGAΞHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI ₂68

+ P P+ + P +++ +TT T T P I

Sbjct: 3199 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 3258

Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328

+ P T P T + T +P T T T + T++ P Sbjct: 3259 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 3315

Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385

Q P + TT P+ GT + T + T TP T PI Sbjct: 3316 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3375

Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443

T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 3376 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 3434

Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502

+ p ++ + +p p +τ + + p+ + PT p+

Sbjct: 3435 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 3483

Query: 503 TYTPITSSVS-TIRQYPVSAQAPNΞA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560

T TPIT++ + T P Q P + IT T V T Q T Sbjct: 3484 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 3541

Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613

P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 3542 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 3600

Query: 614 -PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQΞASTNAPAQGSSPRPSIL 671

P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 3601 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 3660

Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728

T P + TP +T + T P PT Q P T P Sbjct: 3661 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3720

Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788

P+ T P PIT TT P P+ T G+ + P V Sbjct: 3721 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTP 3774

Query: 789 EPMDIMRPVSAVPPLATNTVSPS 811

P P + P T TV+P+ Sbjct: 3775 TPTGTQTPTTT-PITTTTTVTPT 3796

Score = 313 (47.0 bits), Expect = 4.2e-23, P = 4.2e-23 Identities = 169/695 (24%), Positives = 245/695 (35%)

Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154

V P P T + + T V T P TP + + P P PT P Sbjct: 3655 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 3714

Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212

P +T P P G T T + P T +G Q P+ TT + Sbjct: 3715 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 3773

Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268

+ P P+ + P +++ +TT T P I

Sbjct: 3774 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 3833

Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328

+ P T P T + T +P T T T + T++ P Sbjct: 3834 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 3890 Query: 329 AISIQRPAQSRDVTTRITLPΞHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT--VAPILA 385

Q P + TT P+ GT + T + T TP T PI Sbjct: 3891 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3950

Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443

T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 3951 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 4009

Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502

+ P ++ + +P P +T + + P+ + PT P+ Sbjct: 4010 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 4058

Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560

T TPIT++ + T P Q P + IT T V T Q T Sbjct: 4059 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 4116

Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQP 614

P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 4117 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPT- 4174

Query: 615 EGKTSAVVLADGATIVANPISNPFSAAPAATTVVQTHSQSASTNAPAQGSSPRPSILRKK 674

T+ + T+ P P T ++ ++N P + S+P+ S Sbjct: 4175 TTPITTT—TTVTPTPTPTGTQTGPPTHTSTAPIAELTTSNPPPESSTPQTSRSTSS 4229

Query: 675 PATDGAKPKSEIH—VSMATPVTVSMETVSNQNNDQPTIAVPP-TAQQPP—PTIPTMIA 729

P T+ S + + M + S T + T++ PP T PP PT T Sbjct: 4230 PLTESTTLLSTLPPAIEMTSTAPPSTPTAPTTTSGGHTLSPPPSTTTSPPGTPTRGTTTG 4289

Query: 730 AASPPSQPAVALSTI PGAVPITPP—ITTIAAAP-PPSVTVGGSLSSVLGPPVPEI 782

++S P+ V +T P P++ P I T P P ΞV + L+ P E+ Sbjct: 4290 SSSAPTPSTVQTTTTSAWTPTPTPLSTPSIIRTTGLRPYPΞSVLICCVLNDTYYAPGEEV 4349

Score = 279 (41.9 bits), Expect = 1.8e-19, P = 1.8e-19 Identities = 138/540 (25%), Positives = 194/540 (35%)

Query: 278 PVTTSNAIPPAVVATVSATRAQSPVITTTAAH ATDSALSRP—TLSIQHPPSAA 329

P+TT+ + P T + T +P+ TTT T + + P T + P

Sbjct: 1946 PITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP 2005

Query: 330 ISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILAT 386

Q P + TT P+ GT + T + T TP T PI T Sbjct: 2006 TGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTT 2065

Query: 387 NTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSPR 444

T+ P+ T G+ + T P +T T+T P+ + T TT V P T T + Sbjct: 2066 TTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGTQ 2124

Query: 445 IQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAHT 503 p ++ + +p p +τ + + p+ + PT p+ T

Sbjct: 2125 TPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTPT 2173

Query: 504 YTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQGI 561

TPIT++ + T P Q P + IT T V T Q T Sbjct: 2174 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVTP 2231

Query: 562 QPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ— 613

P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 2232 TPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTTT 2290

Query: 614 PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSILR 672

P + V T P + P + T T T +Q+ +T ++ P+ Sbjct: 2291 PITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP 2350

Query: 673 KKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMIA 729

T P + TP +T + T P PT Q P T P Sbjct: 2351 TGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTT 2410

Query: 730 AAΞPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEVE 789

P+ T P PIT TT P P+ T G+ + P V Sbjct: 2411 TTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTPT 2464

Query: 790 PMDIMRPVSAVPPLATNTVSPS 811

P P + P T TV+P+ Sbjct: 2465 PTGTQTPTTT-PITTTTTVTPT 2485

Score = 265 (39.8 bits), Expect = 5.8e-18, P = 5.8e-18 Identities = 179/746 (23%), Positives = 257/746 (34%)

Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154

V P P T + + T V T P TP + + P P PT P Sbjct: 3678 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 3737 Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212

P +T P P G T T + P T +G Q P+ TT V + Sbjct: 3738 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 3796

Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268

+ P P+ + P +++ +TT T T P I

Sbjct: 3797 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 3856

Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328

+ P T P T + T +P T T T + T++ P Sbjct: 3857 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 3913

Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFΞTGTPVAAAT—VAPILA 385

Q P + TT P+ GT + T + T TP T PI Sbjct: 3914 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3973

Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSΞHATAVTTSNIPVAKVVPQQITHTSP 443

T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 3974 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 4032

Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502

+ P ++ + +P P +T + + P+ + PT P+ Sbjct: 4033 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 4081

Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560

T TPIT++ + T P Q P + IT T V T Q T Sbjct: 4082 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 4139

Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQP 614

P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 4140 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTGPP 4198

Query: 615 EGKTSAVVLADGATIVANPISNPFSAAPA ATTVVQTHSQSA-STNAPA—QGSSPRP 668

TS +A+ T +NP P S+ P +T+ T S + ST PA S+ P Sbjct: 4199 T-HTSTAPIAELTT—SNP—PPESSTPQTSRSTSΞPLTESTTLLSTLPPAIEMTSTAPP 4253

Query: 669 SILRKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVPPTAQQPPPTIPTMI 728

S T G S + +P + ++ PT + T T PT Sbjct: 4254 STPTAPTTTSGGHTLSPPPSTTTSPPGTPTRGTTTGSSSAPTPSTVQTTTTSAWT-PTPT 4312

Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSΞVLGPPVPEIKVKEEV 788

++P L P +V I + AP V G+ + E

Sbjct: 4313 PLSTPSIIRTTGLRPYPSSVLICCVLNDTYYAPGEEV-YNGTYGDTCYFVNCSLSCTLEF 4371

Query: 789 EPMDIMRPVSAVPPLATNTVSPSLALLANNLSMPTSDLPPGASPRKKPRKQQH 841

S P + +T +PS ++ S PT P P P +Q++ Sbjct: 4372 YNWSCPSTPSPTPTPSKSTPTPΞKP—SSTPSKPTPGTKPPECPDFDPPRQEN 4422

Score = 254 (38.1 bits), Expect = 8.7e-17, P = 8.7e-17 Identities = 167/697 (23%), Positives = 245/697 (35%)

Query: 115 ΞATPVAVTAPPAHLTPAVPLSFSEGLMKPPPK--PTMPSR-PIAPAPPSTLSLPPKV-PG 170

S + T PP TP+ P + + PPP P+ P+ PI P P ST +LPP P Sbjct: 1587 SPPTITTTTPPPTTTPSPPTTTTT TPPPTTTPSPPTTTPITP-PTSTTTLPPTTTPS 1642

Query: 171 QVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMSIIRSNAPGPPLHIGASHL 230

T + P + P T + + TT I + P PP + Sbjct: 1643 PPPTTTTTPPPTTTPSPPTTTTPSPPITTTTTPPPTTTPSSPI—TTTPSPPTTTMTTPS 1700

Query: 231 PRGAAAAAVMSSSKVTTVLRPTSQLPNAATAQPAVQHIIHQPIQS-RPPVTTSNAIPPAV 289

P SS +TT P+S + P P + PP TT +PP Sbjct: 1701 P TTTPSSPITTTTTPSS TTTPSPPPTTMTTPSPTTTPSPPTTTMTTLPPTT 1751

Query: 290 VATVSATRAQSPVITT-TAAHATDSALSRPTLΞIQH PPSAAISIQRPAQSRDVTTR 344

++ T P IT T + + + + P + + + S + +P ++ Sbjct: 1752 TSSPLTTTPLPPSITPPTFSPFSTTTPTTPCVPLCNWTGWLDSGKPNFHKPGGDTELIGD 1811

Query: 345 ITLPSHPALGTPKQQLHTMAQKTIFΞTGTPVAAATVAPILATN TIPSATTAGS 397

+ P A + + ++ I G V ++ N IP A Sbjct: 1812 VCGPGWAANISCRATMYP—DVPIGQLGQTVVCDVSVGLICKNEDQKPGGVIPMAFCLNY 1869

Query: 398 VSHTQAPTSTI—VTMTVPSHSSHATAVTTSNIPVAKVVPQQITHTΞPRIQPDYPAERSS 455

+ Q TMT + + + T TT+ I V T T + P ++

Sbjct: 1870 EINVQCCECVTQPTTMTTTT-TENPTPPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTT 1928

Query: 456 LIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAHTYTPITSSVS-T 513

+ +P P +T + + P+ + PT P+ T TPIT++ + T Sbjct: 1929 TVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG— QTPTTTPITTTTTVT 1977

Query: 514 IRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQGIQPAPISTQGIQ 572 P Q P + IT T V T Q T P P TQ

Sbjct: 1978 PTPTPTGTQTPTTTPITTTTTVTPTPTP— GTQTPTTTPITTTTTVTPTPTPTGTQTPT 2035

Query: 573 PAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ—PEGKTSAVVLA 624

PI T P P GTQ + TPI T P P GTQ P P T+ V Sbjct: 2036 TTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPT 2094

Query: 625 DGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSILRKKPATDGAKPK 683

T P + P + T T T +Q+ +T ++ P+ T P Sbjct: 2095 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 2154

Query: 684 SEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMIAAASPPSQPAVA 740

+ TP +T + T P PT Q P T P P+ Sbjct: 2155 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTG 2214

Query: 741 LSTIPGAVPITPPITTIAAAPPPSVTVGGΞLSSVLGPPVPEIKVKEEVEPMDIMRPVSAV 800

T P PIT TT P P+ T G+ + P V P P + Sbjct: 2215 TQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTPTPTGTQTPTTT- 2267

Query: 801 PPLATNTVSPS 811

P T TV+P+ Sbjct: 2268 PITTTTTVTPT 2278

Score = 243 (36.5 bits), Expect = 1.3e-15, P = 1.3e-15 Identities = 110/406 (27%), Positives = 154/406 (37%)

Query: 121 VTAP-PAHLTPAVPLSFSEGLMKPPPKPTMPSRPIAPAPPSTLSLPPKVPGQVTVTMESS 179

+T P P TP+ P + + L P P+ P+ PP+T PP T + ++ Sbjct: 1396 ITTPSPPTTTPSPPPTTTTTL-PPTTTPSPPTTTTTTPPPTTTPSPPITT—TTTPLPTT 1452

Query: 180 IPQASAIPVATISGQQGHPSNLHHIMTTNVQMSIIRSNAPGPPLHIGASHLPRGAAAAAV 239

P P++T + P+ TT + P PP + P Sbjct: 1453 TPSP PISTTTTPP—PTTTPSPPTTTPSPP TTTPSPPTTTTTTPPP TT 1498

Query: 240 MΞSSKVTTVLRP TSQLPNAATAQPAVQHIIHQPIQSRP-PVTTSNAIPPAVVATVSA 295

S +TT + P T+ LP T P P + P P TT+ PP T+ Sbjct: 1499 TPSPPMTTPITPPASTTTLPPTTTPSPPTTTTTTPPPTTTPSPPTTTPITPPTSTTTLPP 1558

Query: 296 TRAQSPVITTTAAHATDSALSRPTLSIQHPPSAAISIQRPAQSRDV-TTRITLPSHPALG 354

T SP TTT + S PT + PP+ + P + TT T P P Sbjct: 1559 TTTPSPPPTTTTTPPPTTTPSPPTTTTPSPPTITTTTPPPTTTPSPPTTTTTTP—PPTT 1616

Query: 355 TPKQQLHTMAQKTIFSTGTPVAAATVAPILATNTIPSATTAGSVSHTQAPTSTIVTMTVP 414

TP T +T P T +P T T P TT S T P+ I T T P Sbjct: 1617 TPSPPTTTPITPPTSTTTLP-PTTTPSPPPTTTTTPPPTTTPSPPTTTTPSPPITTTTTP 1675

Query: 415 ΞHSSHATA-VTTSNIPVAKVVPQQITHTSPRIQPDYPAERSSLIPISGHRASPNPVAMET 473

++ ++ +TT+ P + T SP P P ++ P S SP P M T Sbjct: 1676 PPTTTPSSPITTTPSPPTTTM TTPSPTTTPSSPITTTTT-PSSTTTPSPPPTTMTT 1730

Query: 474 RSDNR-PSVPVQFQYFLPTYPPSAYPLAAHTYTPITSSVSTIRQYPVSAQAPNS 526

S PS P LP S+ PL T TP+ S++ P S P + Sbjct: 1731 PSPTTTPSPPTTTMTTLPPTTTSS-PL TTTPLPPSITPPTFSPFSTTTPTT 1780

Score = 189 (28.4 bits). Expect = 8.0e-09, P = 8.0e-09 Identities = 92/374 (24%), Positives = 133/374 (35%)

Query: 439 THTSPRIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYF-LPTYPPSAY 497

T + P P P ++ +P + + P PS P+ LPT PS

Sbjct: 1398 TPSPPTTTPSPPPTTTTTLPPTTTPSPPTTTTTTPPPTTTPSPPITTTTTPLPTTTPSP- 1456

Query: 498 PLAAHTYTPITSSVSTIRQYPVSAQAPNSAITAQTGVGVASTVHLNPMQL-MTVDASHAR 556

P++ T P T++ S P S T T +T PM +T AS Sbjct: 1457 PISTTTTPPPTTTPΞPPTTTPSPPTTTPSPPTTTTTTPPPTTTPSPPMTTPITPPASTTT 1516

Query: 557 HIQGIQPAPISTQGIQPAPIGTPGIQPAPLGTQGIHΞATPINTQGLQPAPMGTQQPQPEG 616

P+P +T P P TP +P T I P +T L P T P P Sbjct: 1517 LPPTTTPSPPTTTTTTPPPTTTP SPPTTTPI—TPPTSTTTLPP TTTPSPPP 1566

Query: 617 KTSAVVLADGATIVANPISNPFSAAPAATTVVQTHΞQSAΞTNAP—AQGSSPRPSILRKK 674

T+ T +P P + P+ T+ T +T +P ++P P+ Sbjct: 1567 TTTTT PPPTTTPΞP PTTTTPSPPTITTTTPPPTTTPSPPTTTTTTPPPTTTPSP 1620

Query: 675 PATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAV-PPTAQQPPPTIPTMIAA—A 731

P T P + + P T + PT PPT P P I T Sbjct: 1621 PTTTPITPPTS— TTLPPTTTPSPPPTTTTTPPPTTTPSPPTTTTPSPPITTTTTPPPT 1678

Query: 732 SPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPV PEIKVK 785

+ PS P + P TP TT ++P + T S ++ PP P Sb_jct: 1679 TTPSSPITTTPSPPTTTMTTPSPTTTPSSPITTTTTPSSTTTPSPPPTTMTTPSPTTTPS 1738 Query: 786 EEVEPMDIMRPVSAVPPLATNTVSPSL 812

M + P + PL T + PS+ Sb_jct: 1739 PPTTTMTTLPPTTTSSPLTTTPLPPSI 1765

Score = 185 (27.8 bits), Expect = 1.6e-09, P = 1.6e-09 Identities = 71/270 (26%), Positives = 99/270 (36%)

Query: 563 PAPIΞTQGIQPAPIGTPGIQPAPLGTQGIHSATP INTQGLQPAPMGTQQPQ PEG 616

P+P +T P P TP P T + + TP I+T P P T P P Sbjct: 1422 PSPPTTTTTTPPPTTTPS-PPITTTTTPLPTTTPSPPISTT-TTPPPTTTPSPPTTTPSP 1479

Query: 617 KTSAVVLADGATIVANPISNPFSAAPAATTVVQTHSQSASTNAPAQGSSPRPSILRKKPA 676

T+ T P + P +P TT + T S +T P SP + P Sbjct: 1480 PTTTPSPPTTTTTTPPPTTTP SPPMTTPI-TPPASTTTLPPTTTPSPPTTTTTTPPP 1535

Query: 677 TDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVPPTAQQPPPTIPTMIAAASPPSQ 736

T P + TP+T T + P+ P T PPPT + PS Sbjct: 1536 TTTPSPPT TTPITPPTSTTTLPPTTTPS-PPPTTTTTPPPTTTPSPPTTTTPSP 1588

Query: 737 PAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSΞVLGPPVPEIKVKEEVEPMDIMRP 796

P + +T P +PP TT PPP+ T ++ + PP + P P Sbjct: 1589 PTITTTTPPPTTTPSPPTTT-TTTPPPTTTPSPPTTTPITPPTSTTTLPPTTTPSP—PP 1645

Query: 797 VSAVPPLATNTVSPSLALLANNLSMPTSDLPPGASP 832

+ P T T SP + T+ PP +P Sbjct: 1646 TTTTTPPPTTTPSPPTTTTPSPPITTTTTPPPTTTP 1681

Score = 183 (27.5 bits), Expect = 3.4e-09, P = 3.4e-09 Identities = 91/390 (23%), Positives = 139/390 (35%)

Query: 326 PSAAISIQRPAQSRDVTTR-ITLPSHPALGTPKQQLHTMAQKTIFSTGTPVAAATVAPIL 384

PS + P + T T PS P T T I +T TP+ T +P + Sbjct: 1399 PSPPTTTPSPPPTTTTTLPPTTTPSPPTTTTTTPPPTTTPSPPITTTTTPLPTTTPSPPI 1458

Query: 385 ATNTIPSATTAGSVSHTQAPTSTIVTMTVPSHSSHATAVTTSNIP—VAKVVPQQITHTS 442

+T T P TT S T P+ T + P+ ++ TT+ P + P T T Sbjct: 1459 STTTTPPPTTTPSPP-TTTPSPPTTTPSPPTTTTTTPPPTTTPSPPMTTPITPPASTTTL 1517

Query: 443 PRIQPDYPAERSSLIPISGHRASP NPVAMETRSDNRP--SVPVQFQYFLPTYPPSAY 497

P P ++ P SP P+ T + P + P T PP+ Sbjct: 1518 PPTTTPSPPTTTTTTPPPTTTPSPPTTTPITPPTΞTTTLPPTTTPSPPPTTTTTPPPTTT 1577

Query: 498 PLAAHTYTPITSSVSTIRQYPVSAQAPNSAITAQTGVGVASTVHLNPMQL-MTVDASHAR 556

P T TP +++T P + +P T T +T P +τ s Sbjct: 1578 PSPPTTTTPSPPTITTTTPPPTTTPΞPP TTTTTTPPPTTTPSPPTTTPITPPTSTTT 1634

Query: 557 HIQGIQPAPISTQGIQPAPIGTPGIQPAPLGTQGIHSATPINTQGLQPAPMGTQQPQPEG 616

P+P T P P TP P P T T T P P Sbjct: 1635 LPPTTTPSPPPTTTTTPPPTTTPS—P-PTTTTPSPPITTTTTPPPTTTPSSPITTTPSP 1691

Query: 617 KTSAVVLADGATIVANPISNPFSAAPAATTVVQTHSQSASTNAPAQGSSPRPSILRKKPA 676

T+ + T ++PI+ + P++TT + +T +P SP + + P Sbjct: 1692 PTTTMTTPSPTTTPSSPITT—TTTPSSTTTPSPPPTTMTTPΞPTTTPSPPTTTMTTLPP 1749

Query: 677 TDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVPP 715

T + P + + P +++ T S + PT P Sbjct: 1750 TTTSSPLT TTPLPPSITPPTFSPFSTTTPTTPCVP 1784

Score = 176 (26.4 bits), Expect = 1.8e-07, P = 1.8e-07 Identities = 101/402 (25%), Positives = 142/402 (35%)

Query: 345 ITLPSHPALGTPKQQLHTMAQKTIFSTGTPVAAATVAPILATNTIPSATTAGΞVSHTQAP 404

IT PS P TP T +T +P T P T P TT + T P Sbjct: 1396 ITTPSPPTT-TPSPPPTTTTTLPPTTTPSPPTTTTTTPPPTTTPSPPITTTTTPLPTTTP 1454

Query: 405 TSTIVTMTVPSHSSHATAVTTS-NIPVAKVVPQQITHTSPRIQPDYPAERΞSLIPISGHR 463

+ I T T P ++ + TT+ + P P T T+P P PI+

Sbjct: 1455 SPPISTTTTPPPTTTPSPPTTTPSPPTTTPSPPTTTTTTP—PPTTTPSPPMTTPITPP- 1511

Query: 464 ASPNPVAMETRSDNRPSVPVQFQYFLPTYPPSAYPLAAHTYTPITSSVSTIRQYPVSAQA 523

AS + T PS P T PP+ P + T TPIT ST P + + Sbjct: 1512 ASTTTLPPTTT PΞPPTTTT TTPPPTTTP-SPPTTTPITPPTΞTTTLPPTTTPS 1563

Query: 524 PNSAITAQ TGVGVASTVHLNPMQLMTVDASHARHIQGIQPAPISTQGIQPAPIGTP 579

P T T +T +P + T P+P +T P P TP

Sbjct: 1564 PPPTTTTTPPPTTTPSPPTTTTPSPPTITTTTPPPTT TPSPPTTTTTTPPPTTTP 1618

Query: 580 G IQPAPLGTQGIHSAT PINTQGLQPAPMGTQQPQPEGKTSAVVLADGATIV 630

I P P T + T P T P P T P S + Sbjct: 1619 SPPTTTPITP-PTSTTTLPPTTTPSPPPTTTTTPPPTTTPΞPPTTTTPΞPPITTTTTPPP 1677 Query: 631 ANPISNPFSAAPAA-TTVVQTHSQSASTNAP-AQGSSPRPSILRKKPATDGAKPKSEIHV 688

S+P + P+ TT + T S + + ++P ++P + P T P Sbjct: 1678 TTTPΞSPITTTPSPPTTTMTTPSPTTTPSSPITTTTTPSSTTTPSPPPTTMTTPSP T 1734

Query: 689 SMATPVTVSMETVSNQNNDQPTIAVPPTAQQPPPTIPTMIAAASPPSQPAVALSTIPG 746

+ +P T +M T+ P P PPT + + P+ P V L G Sbjct: 1735 TTPSPPTTTMTTLPPTTTSSPLTTTPLPPSITPPTFSPF--STTTPTTPCVPLCNWTG 1790

Score = 168 (25.2 bits), Expect = 9.3e-08, P = 9.3e-08 Identities = 89/387 (22%), Positives = 133/387 (34%)

Query: 448 DYPAERΞSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFLPTYPPSAYPLAAHTYTPI 507

DY + P+ +P+P T + + P P PT PS P T P Sbjct: 1381 DYKIRVNCCWPMDKCITTPSP PTTTPSPP—PTTTTTLPPTTTPSP-PTTTTTTPPP 1434

Query: 508 TSSVS TIRQYPVSAQAPNSAITAQTGVGVASTVHLNPMQLMTVDASHARHIQGIQPA 564

T++ S T P+ P+ 1+ T +T P T + P+

Sbjct: 1435 TTTPSPPITTTTTPLPTTTPSPPIΞTTTTPPPTTT PSPPTTTPSPPTT TPS 1485

Query: 565 PISTQGIQPAPIGTPGI-QPAPLGTQGIHSATPINTQGLQPAPMGTQQPQ PEGKTSA 620

P +T P P TP P+ + P T P T P P T+ Sbjct: 1486 PPTTTTTTPPPTTTPSPPMTTPITPPASTTTLPPTTTPSPPTTTTTTPPPTTTPSPPTTT 1545

Query: 621 VVLADGATIVANPISNPFΞAAPAATTVVQTHSQSA-ΞTNAPAQGS ΞPRPSILRKKP 675

+ +T P + P TT T + S +T P+ + +P P+ P Sbjct: 1546 PITPPTSTTTLPPTTTPSPPPTTTTTPPPTTTPSPPTTTTPSPPTITTTTPPPTTTPSPP 1605

Query: 676 ATDGAKPKSEIHVS—MATPVTVSMETVSNQNNDQPTIAVPPTAQQPPPTIPTMIAAASP 733

T P Ξ TP+T T + P+ P T PPPT + Sbjct: 1606 TTTTTTPPPTTTPSPPTTTPITPPTSTTTLPPTTTPS-PPPTTTTTPPPTTTPSPPTTTT 1664

Query: 734 PSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGP PVPEIKVKEEVE 789

PS P +T P + PITT + P ++T ++ P P Sbjct: 1665 PSPPITTTTTPPPTTTPSSPITTTPSPPTTTMTTPSPTTTPSSPITTTTTPSSTTTPSPP 1724

Query: 790 PMDIMRPVSAVPPLATNTVSPSLALLANNLSMPTSDLPPGASP 832

P + P P T +L + + T+ LPP +P Sbjct: 1725 PTTMTTPΞPTTTPSPPTTTMTTLPPTTTSSPLTTTPLPPSITP 1767

Score = 154 (23.1 bits), Expect = 2.7e-06, P = 2.7e-06 Identities = 70/277 (25%), Positives = 92/277 (33%)

Query: 565 PISTQGIQPAPIGTPGIQPAPLGTQGIHSATPINTQGLQPAPMGTQQPQPEGKTSAVVLA 624

PIST P P TP P P T + TP P T P P T +

Sbjct: 1457 PISTT-TTPPPTTTPS—P-PTTTPSPPTTTPSPPTTTTTTPPPTTTPSPPMTTP—ITP 1510

Query: 625 DGATIVANPISNPFSAAPAATTVVQTHSQSASTNAP AQGSSPRPSILRKKPATDGA 680

+T P + P TT T + S T P ++ P+ P T Sbjct: 1511 PASTTTLPPTTTPSPPTTTTTTPPPTTTPSPPTTTPITPPTSTTTLPPTTTPSPPPTTTT 1570

Query: 681 KPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVPPTAQQ—PPPTIPTMIAAASPPSQPA 738

P S T T S T++ T PPT PPPT T + P P Sbjct: 1571 TPPPTTTPSPPTTTTPSPPTITTTTPPPTTTPSPPTTTTTTPPPTT-TPSPPTTTPITPP 1629

Query: 739 VALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEVEPMDIMRPVS 798

+ +T+P +PP TT PPP+ T ++ PP+ +

Sbjct: 1630 TSTTTLPPTTTPSPPPTT-TTTPPPTTTPSPPTTTTPSPPITTTTTPPPTTTPSSPITTT 1688

Query: 799 AVPPLATNTV SPSLALLANNL--SMPTSDLPPGASPRKKP 836 pp T T +PS + S T PP P Sbjct: 1689 PSPPTTTMTTPSPTTTPSSPITTTTTPSSTTTPSPPPTTMTTPSP 1733

Score = 148 (22.2 bits), Expect = l.le-05, P = l.le-05 Identities = 62/254 (24%), Positives = 89/254 (35%)

Query: 583 PAPLGTQGIHSATPINTQGLQPAPMGTQQPQPEGKTSAV VLADGATIVANPISNP 637

P+P T S P T L P T P P T+ + T P+

Sbjct: 1399 PSPPTTTP—SPPPTTTTTLPP TTTPSPPTTTTTTPPPTTTPSPPITTTTTPLPTT 1452

Query: 638 FSAAPAATTVVQTHSQSASTNAPAQGSSPRPSILRKKPATDGAKPKΞEIHVS—MATPVT 695

+ P +TT T + + + P SP P+ P T P S M TP+T Sbjct: 1453 TPSPPISTTT—TPPPTTTPSPPTTTPSP-PTTTPSPPTTTTTTPPPTTTPSPPMTTPIT 1509

Query: 696 VSMETVSNQNNDQPTIAVPPTAQQPPPTIPTMIAAASPPSQPAVALSTIPGAVPITPPIT 755

T + P+ T PP T P+ + P P + +T+P +PP T Sbjct: 1510 PPASTTTLPPTTTPSPPTTTTTTPPPTTTPS—PPTTTPITPPTSTTTLPPTTTPSPPPT 1567

Query: 756 TIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEVEPMDIMRPVSAVPPLATNTVSPSLALL 815

T PPP+ T ++ PP + PP T P+ +

Sbjct: 1568 T-TTTPPPTTTPSPPTTTTPSPPTITTTTPPPTTTPSPPTTTTTTPPPTTTPSPPTTTPI 1626 Query: 816 ANNLSMPTSDLPPGASPRKKP 836

S T+ LPP +P P Sb_jct: 1627 TPPTS—TTTLPPTTTPSPPP 1645

Score = 131 (19.7 bits), Expect = 1.2e-03, P = 1.2e-03 Identities = 112/492 (22%), Positives = 174/492 (35%)

Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154

V P P T + + T V T P TP + + P P PT P Sbjct: 3977 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 4036

Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMΞ 212

P +T P P G T T + P T +G Q P+ TT V + Sbjct: 4037 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 4095

Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268

+ P P+ + P +++ +TT T T P I

Sbjct: 4096 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 4155

Query: 269 IHQPIQSRPPVTTSNAIPPA—VVATVΞATRAQSPVITTTA—AHATDSALΞRPTLSIQH 324

+ P T P + T + T +P T T H + + ++ T Ξ Sbjct: 4156 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTGPPTHTSTAPIAELTTSNPP 4215

Query: 325 PPSAAISIQRPAQS—RDVTTRI-TLPSHPALGTPKQQLHTMAQKTIFSTGTPVAAATVA 381

P S+ R S + TT + TLP PA+ + T T + T T++ Sbjct: 4216 PESSTPQTΞRSTSSPLTESTTLLSTLP—PAI EMTSTAPPSTPTAPTTTSGGHTLS 4269

Query: 382 PILATNTIPSAT-TAGSVS-HTQAPTSTIVTMTVPSHSΞHATAVTTSNIPVAKVVPQQIT 439

P +T T P T T G+ + + APT + V T S A T + P++ P I Sbjct: 4270 PPPSTTTSPPGTPTRGTTTGSSSAPTPSTVQTTTTS AWTPTPTPLS—TPSIIR 4321

Query: 440 HTSPRIQPDYPAERSSLIPISGHRASPNP-VAMETRSDN RPSVPVQFQYFLPTYP- 493

T ++P YP+ ++ +P V T D S+ +++ + P Sbjct: 4322 TTG—LRP-YPSSVLICCVLNDTYYAPGEEVYNGTYGDTCYFVNCSLSCTLEFYNWSCPS 4378

Query: 494 -PSAYPLAAHTYTPITSSVSTIRQYPVSAQAPNSAITAQTGVGVASTVHLNPMQLMTVDA 552

PS P + + TP S S+ P P T L + T

Sbjct: 4379 TPSPTPTPSKS-TPTPSKPSSTPSKPTPGTKPPECPDFDPPRQENETWWLCDCFMATCKY 4437

Query: 553 SHARHIQGIQ PAPISTQGIQPAPIGTP 579

++ I ++ P P + G+QP + P Sbjct: 4438 NNTVEIVKVECEPPPMPTCSNGLQPVRVEDP 4468

Score = 117 (17.6 bits), Expect = 1.8e-02, P = 1.8e-02 Identities = 41/156 (26%), Positives = 55/156 (35%)

Query: 710 TIAVPPTAQQPPPTIPTMIAAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGG 769

T + P T PPPT T + + PS P +T P +PPITT P P+ T Sbjct: 1398 TPSPPTTTPSPPPTTTTTLPPTTTPSPPTTTTTTPPPTTTPSPPITT-TTTPLPTTTPSP 1456

Query: 770 SLSSVLGPPVPEIKVKEEVEPMDIMRPVSAVPPLATNTVSPSLALLANNLSMPTSDLPPG 829

+S+ PP P P + P T T SP T+ PP

Sbjct: 1457 PISTTTTPP PTTTPSPPTTTPSPPTTTPSPPTTTTTTP-PPTTTPSPPM 1504

Query: 830 ASPRKKPRKQQHVISTEEGDMMETNΞTDDEKSTAKS 865

+P P + T T +T +T S Sbjct: 1505 TTPITPPASTTTLPPTTTPSPPTTTTTTPPPTTTPS 1540

Score = 61 (9.2 bits), Expect = 1.6e-09, P = 1.6e-09 Identities = 23/93 (24%), Positives = 41/93 (44%)

Query: 397 SVSHTQAPTSTIVTMTVPSHSSHATAVTTSNIPVAKVV PQQITHTSPRIQPDYPAE 452

S++ + +T T+T+P+ + T TT+ P + V P+ S I D+P+ Sbjct: 1257 SITTRPSTLTTFTTITLPTTPTSFTTTTTTTTPTSSTVLSTTPKLCCLWSDWINEDHPSS 1316

Query: 453 RSΞ LIPISGHRASPNPVAMETRSDNRPSVPVQ 484

S P G +P + E RS P + ++ Sbjct: 1317 GSDDGDREPFDGVCGAPEDI—ECRSVKDPHLSLE 1349

Score = 50 (7.5 bits), Expect = 8.0e-09, P = 8.0e-09 Identities = 16/41 (39%), Positives = 19/41 (46%)

Query: 334 RPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKTIFSTGTP 374

RP+ TT ITLP+ P T T T+ ST TP Sbjct: 1261 RPSTLTTFTT-ITLPTTPTSFTTTTTTTTPTSSTVLST-TP 1299

Score = 46 (6.9 bits), Expect = 5.4e-08, P = 5.4e-08 Identities = 24/106 (22%), Positives = 37/106 (34%)

Query: 324 HPPSAAISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKTIFSTGTPVAAATVAPI 383 +PP A++ + +S T + P G Q A G I Sbjct 1196 YPPGASVPTEETCKSCVCTNSSQVVCRPEEGKILNQTQDGAFCYWEICGPNGTVEKHFNI 1255 Query 384 LATNTIPSA-TTAGSVSHTQAPTSTIVTMTVPSHSSHATAVTTSNI 428

+ T PS TT +++ PTS T T + +S TT + Sbjct 1256 CSITTRPSTLTTFTTITLPTTPTSFTTTTTTTTPTSSTVLSTTPKL 1301

Score = 44 (6.6 bits), Expect = 8.7e-08, P = 8.7e-08 Identities = 14/34 (41%), Positives = 17/34 (50%)

Query: 478 RPSVPVQFQYF-LPTYPPΞAYPLAAHTYTPITSSV 511

RPS F LPT P S + T TP +S+V Sbjct: 1261 RPSTLTTFTTITLPTTPTS-FTTTTTTTTPTSSTV 1294

Pedant information for DKFZphtes3_2all, frame 2

Report for DKFZphtes3_2all .2

[LENGTH] 1048

[MW] 110324.04

[pi] 9.83

[HOMOL] PIR: 147141 gastric mucin (clone PGM-2A) - pig (fragment) 8e-15

[FUNCAT] 30.90 extracellular/secretion proteins [S. cerevisiae, YIR019c] le-09

[FUNCAT] 30.01 organization of cell wall [S. cerevisiae, YIR019c] le-09

[FUNCAT] 01.05.01 carbohydrate utilization [S. cerevisiae, YIR019c] le-09

[FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YDR420w] 4e-09

[FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YDR420w]

4e-09

[FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YJR151c] 4e-06

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YGR014w] le-05

[FUNCAT] 11.01 stress response [S. cerevisiae, YHL028w] le-04

[FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YHL028w] le-04

[EC] 3.2.1.3 Glucan 1, 4-alpha-glucosιdase 3e-08

[PIRKW] glycosidase 3e-08

[PIRKW] transmembrane protein 3e-08

[PIRKW] polysaccharide degradation 3e-08

[PIRKW] glycoprotein 9e-08

[PIRKW] calcium binding 9e-08

[PIRKW] hydrolase 3e-08

[PIRKW] cytoskeleton 7e-08

[SUPFAM] equine herpesvirus glycoprotein X 2e-07

[SUPFAM] yeast glucan 1, 4-alpha-glucosιdase homolog 3e-08

[SUPFAM] polymorphic epithelial mucin 7e-08

[SUPFAM] glucan 1, 4-alpha-glucosιdase homology 3e-08

[SUPFAM] equine herpesvirus 1 glycoprotein homology 2e-07

[PROSITE] MYRISTYL 9

[PROSITE] AMIDATION 1

[PROSITE] CAMP_PHOSPHO_SITE 2

[PROSITE] CK2_PHOSPHO_SITE 10

[PROSITE] PKC_PHOSPHO_SITE 12

[PROSITE] ASN_GLYCOSYLATION 3

[KW] Irregular

[KW] LOW COMPLEXITY 20.04 %

SEQ MGPPRHPQAGEIEAGGAGGGRRLQVEMSSQQFPRLGAPSTGLSQAPSQIANΞGSAGLINP

SEG xxxxxxxxxxxx

PRD ccccccccccccccccccccceeeeeeccccccccccccccccccccccccccccccccc

SEQ AATVNDESGRDSEVSAREHMSSSSSLQSREEKQEPVVVRPYPQVQMLSTHHAVASATPVA

SEG xxxxx xxxxxxxxxxxx

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ VTAPPAHLTPAVPLSFSEGLMKPPPKPTMPSRPIAPAPPSTLSLPPKVPGQVTVTMESSI

SEG xxxxxxxxxxxxx xxxxxxxxx..xxxxxxxxxxxxxx

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccceeeccccc

SEQ PQAΞAIPVATISGQQGHPΞNLHHIMTTNVQMSIIRΞNAPGPPLHIGASHLPRGAAAAAVM SEG xxxxx .. PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ SSSKVTTVLRPTSQLPNAATAQPAVQHIIHQPIQSRPPVTTΞNAIPPAVVATVSATRAQS SEG

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ PVITTTAAHATDSALSRPTLSIQHPPSAAISIQRPAQSRDVTTRITLPSHPALGTPKQQL SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ HTMAQKTIFSTGTPVAAATVAPILATNTIPSATTAGSVSHTQAPTSTIVTMTVPSHSSHA SEG xxxxxxxxxx xxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ TAVTTSNIPVAKVVPQQITHTSPRIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPS SEG xxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccceeeecccccccc

SEQ VPVQFQYFLPTYPPSAYPLAAHTYTPITSSVSTIRQYPVSAQAPNSAITAQTGVGVASTV SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ HLNPMQLMTVDASHARHIQGIQPAPISTQGIQPAPIGTPGIQPAPLGTQGIHSATPINTQ SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ GLQPAPMGTQQPQPEGKTSAVVLADGATIVANPISNPFSAAPAATTVVQTHSQSASTNAP SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ AQGSSPRPSILRKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVPPTAQQP SEG xxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ PPTIPTMIAAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVP SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccceeeccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ EIKVKEEVEPMDIMRPVSAVPPLATNTVSPSLALLANNLSMPTSDLPPGASPRKKPRKQQ SEG xxxxxxxxxx xxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ HVISTEEGDMMETNSTDDEKSTAKSLLVKAEKRKSPPKEYIDEEGVRYVPVRPRPPITLL SEG xxxxxxxxxx .... PRD ccccccccccccccccccccchhhhhhhhhccccccccccccccccccccccccccccee

SEQ RHYRNPWKAAYHHFQRYSDVRVKEEKKAMLQEIANQKGVSCRAQGWKVHLCAAQLLQLTN SEG PRD eeccccchhhhhhhccccchhhhhhhhhhhhhhhhhccceeecccceeehhhhhhhhhhc

SEQ LEHDVYERLTNLQEGIIPKKKAATDDDLHRINELIQGNMQRCKLVMDQISEARDSMLKVL SEG PRD cchhhhhhhhhhhceeeeccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ DHKDRVLKLLNKNGTVKKVSKLKRKEKV SEG xxxxxxxxxxxxx PRD hhhhhhhhhhccccceeeeeeeeccccc

Prosite for DKFZphtes3_2all .2

PS00001 818->822 ASN_GLYCOSYLATION PDOC00001 PS00001 854->858 ASN_GLYCOSYLATION PDOC00001 PS00001 1033->1037 ASN_GLYCOSYLATION PDOC00001 PS00004 872->876 CAMP_PHOSPHO_SITE PDOC00004 PS00004 1037->1041 CAMP_PHOSPHO_SITE PDOC00004 PS00005 68->71 PKC_PHOSPHO_SITE PDOC00005 PS00005 75->78 PKC_PHOΞPHO_SITE PDOC00005 PS00005 242->245 PKC_PHOSPHO_SITE PDOC00005 PS00005 342->345 PKC_PHOSPHO_SITE PDOC00005 PS00005 355->358 PKC_PHOSPHO_SITE PDOC00005 PS00005 442->445 PKC_PHOSPHO_SITE PDOC00005 PS00005 513->516 PKC_PHOSPHO_SITE PDOC00005 PS00005 665->668 PKC_PHOSPHO_SITE PDOC00005 PS00005 831->834 PKC_PHOSPHO_SITE PDOC00005 PS00005 862->865 PKC_PHOSPHO_SITE PDOC00005 PS00005 940->943 PKC_PHOSPHO_SITE PDOC00005 PS00005 1035->1038 PKC_PHOSPHO_SITE PDOC00005 PS00006 63->67 CK2_PHOSPHO_SITE PDOC00006 PS00006 68->72 CK2_PHOSPHO_SITE PDOC00006 PS00006 75->79 CK2_PHOSPHO_SITE PDOC00006 PS00006 88->92 CK2_PHOΞPHO_SITE PDOC00006 PΞ00006 135->139 CK2_PHOSPHO_SITE PDOC00006 PS00006 473->477 CK2_PHOSPHO_SITE PDOC00006 PS00006 844->848 CK2_PHOSPHO_SITE PDOC00006 PS00006 855->859 CK2_PHOSPHO_SITE PDOC00006 PS00006 959->963 CK2_PHOSPHO_SITE PDOC00006 PS00006 984->988 CK2_PHOSPHO_SITE PDOC00006 PS00008 15->21 MYRISTYL PDOC00008 PS00008 16->22 MYRISTYL PDOC00008 PS00008 36->42 MYRISTYL PDOC00008 PS00008 233->239 MYRISTYL PDOC00008 PS00008 372->378 MYRISTYL PDOC00008 PS00008 533->539 MYRISTYL PDOC00008 PS00008 535->541 MYRISTYL PDOC00008 PS00008 590->596 MYRISTYL PDOC00008 PS00008 768->774 MYRISTYL PDOC00008 PS00009 19->23 AMIDATION PDOC00009

(No Pfam data available for DKFZphtes3_2all .2)

DKFZphtes3_2al7

group: metabolism

DKFZphtes3_2al7 encodes a novel 574 amino acid protein without similarity to known proteins.

The novel protein contains a thiol protease cys pattern. Eukaryotic thiol proteases (EC 3.4.22.-) are a family of proteolytic enzymes containing an active site cysteine. Cathepsins belong to this protease family.

The new protein can find application m modulation of proteolytic processes and as a new enzyme for proteomic analysis and biotechnologic production processes. unknown complete cDNA, complete eds, EST hits

Sequenced by EMBL

Locus: unknown

Insert length: 2312 bp

Poly A stretch at pos. 2300, polyadenylation signal at pos. 2273

1 GTTTTCACCT GATCATTAGA AACTAATGAA ACACCTTTTA AGTCTTATGA 51 ATTCAGGTTA CACTGTTTTC CAGATGCCTT GGCAGCTGGT ACAGGGCCTC

101 TGAAAAATGG AACCAAATTC TCTGAGGACT AAAGTCCCAG CTTTCTTATC

151 TGATTTGGGG AAGGCCACAT TGAGGGGAAT CAGAAAGTGT CCCCGATGTG

201 GCACATACAA TGGAACCCGG GGACTGAGCT GTAAGAACAA GACATGTGGA

251 ACCATATTCC GCTACGGTGC ACGCAAGCAG CCTAGTGTTG AAGCTGTCAA

301 AATCATTACA GGCTCTGATC TTCAGGTCTA CTCAGTGCGG CAAAGAGACC

351 GGGGCCCTGA TTACCGATGC TTTGTGGAGC TCGGGGTTTC AGAGACAACA

401 ATCCAGACAG TGGATGGGAC GATCATCACT CAGCTGAGCT CTGGACGGTG

451 TTATGTCCCC TCATGCCTGA AAGCTGCCAC TCAAGGCGTT GTGGAAAACC

501 AGTGCCAGCA CATCAAGCTG GCGGTGAACT GCCAGGCAGA GGCCACCCCT

551 CTGACCCTGA AGAGCTCGGT CCTGAATGCA ATGCAGGCCT CCCCGGAAAC

601 CAAACAGACC ATCTGGCAGT TGGCCACGGA ACCCACAGGT CCTCTGGTGC

651 AGAGAATTAC TAAAAACATC TTGGTGGTGA AATGCAAGGC AAGCCAGAAG

701 CACAGTTTGG GGTATTTGCA TACATCTTTT GTGCAGAAAG TCAGTGGCAA

751 AAGCTTGCCT GAGCGCCGCT TCTTCTGCTC CTGTCAGACT CTGAAATCGC

801 ACAAGTCAAA TGCCTCCAAG GATGAGACAG CCCAGAGATG CATTCATTTC

851 TTTGCTTGCA TCTGTGCCTT TGCCAGTGAT GAGACACTGG CTCAGGAATT

901 CTCAGACTTC CTAAATTTTG ATTCCAGCGG TCTTAAAGAG ATTATTGTAC

951 CCCAGTTAGG TTGCCATTCA GAATCAACAG TATCTGCTTG TGAGTCTACT 1001 GCCTCTAAGT CAAAGAAGAG GAGAAAGGAT GAAGTATCTG GTGCACAGAT 1051 GAACAGTTCA CTACTGCCTC AAGATGCAGT GAGCAGTAAT CTAAGGAAAA 1101 GTGGCCTGAA AAAGCCTGTG GTTGCTTCCT CGTTAAAAAG GCAGGCCTGT 1151 GGTCAGCTGT TAGATGAGGC ACAAGTGACT TTATCCTTCC AAGACTGGCT 1201 GGCCAGTGTC ACAGAACGCA TCCATCAAAC CATGCACTAT CAGTTTGATG 1251 GCAAACCAGA ACCATTGGTG TTCCACATTC CTCAGTCATT TTTTGATGCC 1301 CTGCAACAAA GAATATCTAT AGGAAGTGCA AAAAAACGGC TCCCCAACTC 1351 CACCACAGCT TTTGTTCGGA AAGATGCCTT GCCACTGGGA ACCTTTTCCA 1401 AGTATACTTG GCATATCACT AATATCCTGC AAGTTAAACA AATCTTAGAT 1451 ACCCCAGAGA TGCCCTTGGA AATCACCCGT AGCTTTATCC AGAACCGAGA 1501 TGGGACTTAT GAGCTATTTA AATGCCCTAA AGTGGAAGTA GAAAGCATAG 1551 CAGAAACCTA CGGTCGTATA GAAAAACAAC CAGTGCTGCG ACCCTTGGAA 1601 CTAAAAACTT TTCTCAAAGT TGGCAACACT TCCCCAGATC AAAAGGAGCC 1651 AACACCTTTC ATCATCGAGT GGATCCCAGA TATCCTTCCC CAATCTAAGA 1701 TTGGCGAGCT GCGGATCAAG TTTGAGTATG GCCACCACCG GAATGGGCAT 1751 GTGGCGGAGT ACCAAGACCA GCGGCCCCCC TTGGACCAGC CCTTGGAACT 1801 GGCCCCTCTG ACCACTATTA CTTTCCCTTA AAGCAAAACA AGATAATAAT 1851 CTTTTGCTGC TTAATTTGCA CATCCCCACC CCTTGACAAC TTTAAATGCT 1901 AGTTAGGCAC TTAGATGGCC CTGTTCCTTG GTAAACTGCT CTTAGCTAAG 1951 ATGCAAATTC TCAGTGCTTT CAAGTGGATT CTGTTGAAGA AAATCTCTTG 2001 TAAATAGCCT TTTTGATGCT GCTGTGTACA GTCTTCATTA TGCATTGGGC 2051 AGTATTTCTG GCTAGAGTTT TAAAAGGAAC AGAAAGAAAA CCAGCTTATT 2101 TTCCTTCTTA CGGACTCATC TTTAGCGTTT ATTTCAACCT TTTGCTAATT 2151 CTCTGAGAAA TCTGCAGCAC TCAGCCATAC ACCAACAGTG TTGGAAAGTT 2201 AACACCCTGG TTAGGGCAGA ATGTTAAAGA CCATCTTGGC AGAGTTCCAG 2251 CCACGCTCTT TATTCTGTTC TCAAATAAAG CAGTGTCACT AGTTTTTCCT 2301 AAAAAAAAAA AA

BLAST Results o BLAST result Medlme entries

No Medlme entry

Peptide information for frame 2

ORF from 107 bp to 1828 bp; peptide length: 574 Category: putative protein

1 MEPNSLRTKV PAFLSDLGKA TLRGIRKCPR CGTYNGTRGL SCKNKTCGTI 51 FRYGARKQPS VEAVKIITGS DLQVYSVRQR DRGPDYRCFV ELGVSETTIQ 101 TVDGTIITQL SSGRCYVPSC LKAATQGVVE NQCQHIKLAV NCQAEATPLT 151 LKSSVLNAMQ ASPETKQTIW QLATEPTGPL VQRITKNILV VKCKASQKHS 201 LGYLHTSFVQ KVSGKSLPER RFFCSCQTLK SHKSNASKDE TAQRCIHFFA 251 CICAFASDET LAQEFSDFLN FDSSGLKEII VPQLGCHSEΞ TVSACESTAS 301 KSKKRRKDEV SGAQMNSSLL PQDAVSSNLR KSGLKKPVVA SSLKRQACGQ 351 LLDEAQVTLS FQDWLASVTE RIHQTMHYQF DGKPEPLVFH IPQSFFDALQ 401 QRISIGSAKK RLPNSTTAFV RKDALPLGTF SKYTWHITNI LQVKQILDTP 451 EMPLEITRSF IQNRDGTYEL FKCPKVEVES IAETYGRIEK QPVLRPLELK 501 TFLKVGNTSP DQKEPTPFII EWIPDILPQS KIGELRIKFE YGHHRNGHVA 551 EYQDQRPPLD QPLELAPLTT ITFP

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_2al7, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphtes3_2al7, frame 2

Report for DKFZphtes3_2al7.2

[LENGTH] 574

[MW] 64076.89

[pi] 9.15

[PROSITE] MYRISTYL 5

[PROSITE] CK2_PHOSPHO_SITE 9

[PROSITE] PKC_PHOSPHO_SITE 14

[PROSITE] ASN_GLYCOSYLATION 5

[PROSITE] THIOL_PROTEASE_CYS 1

[KW] Alpha_Beta

SEQ MEPNSLRTKVPAFLSDLGKATLRGIRKCPRCGTYNGTRGLSCKNKTCGTIFRYGARKQPS

PRD ccccccccccchhhhhcccchhhhhcccccccccccccccccccccccceeeeccccccc

SEQ VEAVKIITGSDLQVYSVRQRDRGPDYRCFVELGVSETTIQTVDGTIITQLSSGRCYVPSC

PRD ceeeeeeecccceeeeeccccccccceeeeeecccccceeeccceeeeeecccccccchh

SEQ LKAATQGVVENQCQHIKLAVNCQAEATPLTLKSSVLNAMQASPETKQTIWQLATEPTGPL

PRD hhhhhhhhcchhhhheeehhhhhhhcccccchhhhhhhhhcccchhhhhhhhhcccccch

SEQ VQRITKNILVVKCKASQKHSLGYLHTSFVQKVSGKSLPERRFFCSCQTLKSHKSNASKDE

PRD hhhhhhheeeeeecccccccccccceeeeeeecccccccceeeecccccccccccccccc

SEQ TAQRCIHFFACICAFASDETLAQEFSDFLNFDSSGLKEIIVPQLGCHSESTVSACESTAS

PRD hhhhhhhhhhhhhhhhhchhhhhhhhhhhccccccceeeeeecccccccceeeccccccc

SEQ KSKKRRKDEVSGAQMNSSLLPQDAVSSNLRKSGLKKPVVASSLKRQACGQLLDEAQVTLS

PRD ccchhhhhccccccccccccccccchhhhhhhccccceeehhhhhhhhhchhhhhhhhhh

SEQ FQDWLASVTERIHQTMHYQFDGKPEPLVFHIPQSFFDALQQRISIGSAKKRLPNSTTAFV

PRD hhhhhhhhhhhhhhhhhhhcccccccceeehhhhhhhhhhhhhhhhcccccccccceeee

SEQ RKDALPLGTFSKYTWHITNILQVKQILDTPEMPLEITRSFIQNRDGTYELFKCPKVEVES

PRD ecccccccccceeeeehhhhhhhhhhhccccccccceeeeeeccccceeeecccceeeeh

SEQ IAETYGRIEKQPVLRPLELKTFLKVGNTSPDQKEPTPFIIEWIPDILPQSKIGELRIKFE

PRD hhhhhhhhhccccccccccceeeeecccccccccccceeeeecccccccccccceeeeee 1313 '^, 1313

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DKFZphtes3_2dl5

group: testes derived

DKFZphtes3_2dl5 encodes a novel 274 amino acid protein with similarity to C. elegans cosmid F25H2.1.

The novel protein contains a Pfam predicted C2-domaιn.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . similarity to C. elegans F25H2.1 complete cDNA, complete eds, EST hits

Sequenced by EMBL

Locus : unknown

Insert length: 3615 bp

Poly A stretch at pos. 3603, polyadenylation signal at pos. 3578

1 GCGGCGGCCT CGAGGTGACA ACTGTCTCCG TCGCAGGCTC CGGCGGGGGC

51 GCAGGAGGTC GCCCGGCGCG TCACTGTCGG GTCGGCGAGC CACGGGGGCC

101 GCCGCAGCAC CATGGCGACC ACCGTCAGCA CTCAGCGCGG GCCGGTGTAC

151 ATCGGTGAGC TCCCGCAGGA CTTCCTCCGC ATCACGCCCA CACAGCAGCA

201 GCGGCAGGTC CAGCTGGACG CCCAGGCGGC CCAGCAGCTG CAGTACGGAG

251 GCGCAGTGGG CACCGTGGGC CGACTGAACA TCACGGTGGT ACAGGCAAAG

301 TTGGCCAAGA ATTACGGCAT GACCCGCATG GACCCCTACT GCCGACTGCG

351 CCTGGGCTAC GCGGTGTACG AGACGCCCAC GGCACACAAT GGCGCCAAGA

401 ATCCCCGCTG GAATAAGGTC ATCCACTGCA CGGTGCCCCC AGGCGTGGAC

451 TCTTTCTATC TCGAGATCTT CGATGAGAGA GCCTTCTCCA TGGACGACCG

501 CATTGCCTGG ACCCACATCA CCATCCCGGA GTCCCTGAGG CAGGGCAAGG

551 TGGAGGACAA GTGGTACAGC CTGAGCGGGA GGCAGGGGGA CGACAAGGAG

601 GGCATGATCA ACCTCGTCAT GTCCTACGCG CTGCTTCCAG CTGCCATGGT

651 GATGCCACCC CAGCCCGTGG TCCTGATGCC AACAGTGTAC CAGCAGGGCG

701 TTGGCTATGT GCCCATCACA GGGATGCCCG CTGTCTGTAG CCCCGGCATG

751 GTGCCCGTGG CCCTGCCCCC GGCCGCCGTG AACGCCCAGC CCCGCTGTAG

801 CGAGGAGGAC CTGAAAGCCA TCCAGGACAT GTTCCCCAAC ATGGACCAGG

851 AGGTGATCCG CTCCGTGCTG GAAGCCCAGC GAGGGAACAA GGATGCCGCC

901 ATCAACTCCC TGCTGCAGAT GGGGGAGGAG CCATAGAGCC TCTGCCTCGA

951 TGCCGTTTTG CCCCCGCTCT TTGGACACGC CGACCCGGCG CTCCCCAAGG

1001 AATGCTGTCC CAACAAGATT CCCGTGAAAG AGCACCCGTG TCGCCCCCTC

1051 CCGTGGACTT CTGTGCCGCC CCGTCCACAC CTGTTCTTGG GTGCATGTGG

1101 GTTTTCGGTT CCTGGCGGTC CAGGACGGGG CGGGGGCTCC CCTCCCATCT

1151 CGTGCTGGGA GGTCTCAGCG CGCTCTCCTG TCCCTGGGAC GTGCGTCTCT

1201 CCTTCTCATG CCGTTCTGGA AAATGCTCTT GCTGTAGAGA GCAGCTGCTT

1251 CTGCCAGGGT GTTGGAGGTG GTGGAGCGCC TTCCGATTCC ATTCATGGCA

1301 TTTTGTGATG TGATGTAATT GGAATAGAGC TGTTGATTTA AGGCACACAC

1351 AATCCCTCAC ACTGTGGGTT TTTTTTAGAA CTTCCCAGAC GAAAACTCAC

1401 GCCCTTGCCC TAACGCGCTT TGCTGTGAGC CTGGCCCCTG CCCAGGGCTT

1451 GGGTCTGGTG AGCTGAGCAG CTTCCTGTGG ATGGTGTGGG GCCGGCCTCT

1501 GGCCTGGCTC ACCTGGCCAC TGTCCAGCCA GCCTTGTGAC AGACTCCGGC

1551 CTGAAGGCAG AATGAACCCA CACCTGGAGT GAGGAAGGGG GCCTGGCACG

1601 GTTGGCCAGG CTCTGCCTGA TTGCCAGCCA GCGGGCATCT GAAGCCGGGT

1651 CCTTCGCCCG CCGGAGGCTG CCGTCCGTCT CTCCTGCTGC GCTCGTGCCA

1701 GCTCCGTGGG TGTCCTCCCA GGGAGCTTCT CTTCTCAACA GGCCTTGCGA

1751 GGCTGGGGTG AGAGGTGATA GAGGCAGCAC TGTGCATGAT TCCGAGAGGG

1801 TGTGGTGGCA CTGCCAGCCG ACTGCTGACA GCTTGGGAGC TGCTGTGCCC

1851 AGGACGTGGG TTCAGCGTGG GCGAGGAAAG CCTGGCGAGC GTGGCCCTGT

1901 AAAAGCTTTC TGAGGCGGGA GGCGCTCACT TACCTCTGAC TGCCTGGGCG

1951 CTGCGTGTAG CATCTTGGCC TACAGGACAG ATTTTAGGTG ACACCTGGTT

2001 ATGACAGTCA GAAATTTGAG AAGCTTCTCA CAAGTGATGC ACTTTAAATA

2051 ATCTGCATGC CATTGAGACA CCTGCATGTC TGGTGTTTGT GGTTCAAGTG

2101 TCTTGCCGCC GGCCTTCGGA TGTAAACCCA CTGATAACGG ACAGAAAGAG

2151 AATGCCCACA AGTGGGTCTT CTGTGGAAGA TGCAGAAGGA GGAAGTTAGT

2201 GCTTACATTT TAGTCTTTTT CTCCCTCAAA AAAATAGGTT AAGTTTCAGT

2251 GCCAGCTAGA AAATACTGCT TTCTGCCATC GATTGGGGGT GGTTTTTGTC

2301 AAATATACTG TTGATAAATA TTTATTTTTG TAAACTTGAA GTGTGTGGTG

2351 GCCGTGGGGG AGGGACATGC TGGCAGCAGG CGCCTTCTTC AGCTGTGGGT

2401 CCTAAAGGCC TTTGATCCTT TGAAGAAGAA AGACATGGTA TTTGTTCAGC

2451 AGACGCCGAC CACTCAGACG GAGGGGCCCC TGGGATTCCC TGTCTCAGAT

2501 GGCCTGGTCT TACGCCTGTG TAGATTTCTT CTCCATTGGG AATGAAGGTG

2551 TCAGGCGGGA CTGGAACGTT CTAGATGGTA TGTTCCGTGA TATTAACAAC

2601 TCTAACCCAG GACAGACCAC AAGCCACACT CAGAGGCCTC ACTGTGCTGG 2651 GGGCTTCGGT GTCCAGGCGC CCAGGTGTGG CCACCAGCAC CGGTTTCTGC 2701 CTTCGCGTTG CTGGGGTGCA GTGAGACTGC CACACGCGTG CACATGTGGC 2751 TCTGTGGGTG TCTCCTAGAG AGGACGTGGC CCCTGCTGCC AGCCCTTGAG 2801 CAGCCCGTGT GGGGGCCCGA GGGACCCACA CAGTGGGGGC CAGCCTCGCT 2851 GGAGGGAGAG CAACCCTTTG CCGATGACCA CGCTTGCCGC CATCTCTTAG 2901 TTTTCTTTTT CACAAGCGCT TTATTTTTTT AATAGACAAA TCACATTTTG 2951 CAAGGCCTTT AATTAAATAA GATTCTTCTT TCCTTCATTT TATGCTTTAT 3001 TTCCTGTTTG AAGGCTTACT GTAGAAGTGG CTTACTGTAG AAGCAGCTTG 3051 CTGAGCCCCT CCGAGCGGTC CCCAGAATTA GCTGGTTCAC AACCCCCACC 3101 CTCCCCCGCC CCCGCCTGTG TCAGGTGTGG ATGAGGTCGT CACACTCAGA 3151 AGGACAGGCT TGTCTGCCAG CTCACAAGGG GAGGCTGCAG TGGGTTTGGG 3201 AGCTGGGTTT AGGCCCCTGG TGTCTGAGGG CCCAGGCCTT GCCAGCCTCT 3251 GCTGCTCCTG CTCCTGGGTT TGAAGATGCA GGCCGATCGC CAGCTCCGTG 3301 GCAGCGGTCA CTAAGGACAG CCTGACTGTG CCATCTTGGA GCCTCAGGCG 3351 GGGCTCCGGA GATAGAAGAC AGGTCGCCGG AGGCTCCCCC TCCTCTCCTC 3401 TCCCCTCTGC AGATGCTCCC TGGGCGCTAC CCTGCAGGGT GCCAGGCAGG 3451 AGTGGTCTCA GAACGTGCGC TTCTGATTAT TTTACTGGGG TCCATTGTCC 3501 AGATTTTTCT TTGATTGTAA AATATATTTT TACTTTTTAG TCTTCTAATT 3551 TAATAAATGA TCCATATAAA AATAGAGAAA TAAAGTCCTT TAAGGGAAGG 3601 TTTAAAAAAA AAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 1

ORF from 112 bp to 933 bp; peptide length: 274 Category: similarity to unknown protein Classification: no clue

1 MATTVSTQRG PVYIGELPQD FLRITPTQQQ RQVQLDAQAA QQLQYGGAVG

51 TVGRLNITVV QAKLAKNYGM TRMDPYCRLR LGYAVYETPT AHNGAKNPRW

101 NKVIHCTVPP GVDSFYLEIF DERAFSMDDR IAWTHITIPE SLRQGKVEDK

151 WYSLSGRQGD DKEGMINLVM SYALLPAAMV MPPQPVVLMP TVYQQGVGYV

201 PITGMPAVCS PGMVPVALPP AAVNAQPRCS EEDLKAIQDM FPNMDQEVIR

251 SVLEAQRGNK DAAINSLLQM GEEP

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_2dl5, frame 1

TREMBL :CEF25H2_1 gene: "F25H2.1"; Caenorhabditis elegans cosmid F25H2, N = 1, Score = 385, P = 1. le-35

>TREMBL:CEF25H2_1 gene: "F25H2.1"; Caenorhabditis elegans cosmid F25H2 Length = 457

HSPs:

Score = 385 (57.8 bits), Expect = 1. le-35, P = 1. le-35 Identities = 77/182 (42%), Positives = 118/182 (64%)

Query: 4 TVSTQRGPVYIGELPQDFLRIT-PTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQA 62

TV+ +R V +GELP FLR+ P QQ + ++ Q + + + T GRL++T+++A Sbjct: 5 TVAERRRQVLVGELPPHFLRLAVPIQQTAEPEI-VQP-RMVSFVPP-NTRGRLSVTILEA 61

Query: 63 KLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDE 122

L KNYG+ RMDPYCR+R+G ++T A + P WN+ ++ +P V+S Y++IFDE Sbjct: 62 NLVKNYGLVRMDPYCRVRVGNVEFDTNVAANAGRAPTWNRTLNAYLPMNVESIYIQIFDE 121

Query: 123 RAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYAL—LPAAMV 180

+AF D+ IAW HI +P ++ G D+++ LSG+QG+ KEGMI+L S+A LP Sbjct: 122 KAFGPDEVIAWAHIMLPLAIFNGDNIDEYFQLSGQQGEGKEGMIHLHFSFAPIDLPLQQA 181 Query: 181 MPPQP 185

P +P Sbjct: 182 APAEP 186

Score = 92 (13.8 bits), Expect = 1.8e-01, P = 1.7e-01 Identities = 26/68 (38%), Positives = 38/68 (55%)

Query: 194 QQGVGYVPITGMPAVCSPGMVPV—ALP--PAAVNAQPRCSEEDLKAIQDMFPNMDQEVI 249

QQG G + + +P +P+ A P PA +EED K IQ+MFP +D+EVI Sbjct: 156 QQGEGKEGMIHLHFSFAPIDLPLQQAAPAEPAPAPLPVEITEEDTKEIQEMFPIVDKEVI 215

Query: 250 RSVLEAQR 257

+ +LE +R Sbjct: 216 KCILEERR 223

Pedant information for DKFZphtes3_2dl5, frame 1

Report for DKFZphtes3_2dl5.1

[LENGTH] 274 [MW] 30281.97 [pi] 5.68 [HOMOL] TREMBL :CEF25H2_1 gene: "F25H2.1"; Caenorhabditis elegans cosmid F25H2 4e-36

[PFAM] C2 domain

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 16.42 %

SEQ MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVV SEG xxxxxxxxxxxxxxxxx

PRD cccccccccceeeeeccccceeeecccchhhhhhhhhhhhhhhhhcccccceeeeceeeh

SEQ QAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIF SEG PRD hhhhhhhhcccccccchhhhheeeeeecccccccccccccceeeeeccccccceeeeeec

SEQ DERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMV SEG xxxxxxxx PRD cccccccccceeeeccccccccccccccceeeeeccccccccccceeeeehhhhhhhhhc

SEQ MPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDM SEG xxxxxxxxxx xxxxxxxxxx PRD ccccceeeeeeeeecccccccccccceeecccccccccccceeeeccccchhhhhhhhhc

SEQ FPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEP SEG PRD ccccchhhhhhhhhhhccccchhhhhhhhhhccc

(No Prosite data available for DKFZphtes3_2dl5.1)

Pfam for DKFZphtes3_2dl5.1

HMM_NAME C2 domain

HMM *LtVrIIeARNLWkMDMnGfSDPYVKVdMdPdpkDtkKWKTkTιWNNGLN L++++++A+ + + M+ DPY+++ + + + +T T +N N

Query 55 LNITVVQAKLAKNYGMT-RMDPYCRLRLGYAVY ETPTAHNGAKN 97

HMM PVWNEEeFvFedlPyPdlqrkMLRFaVWDWDRFSRBDFIGHCi* P+WN + +P + + ++++D+ FS +D 1+ +

Query 98 PRWN-KVIHCT-VPPGVDSF YLEIFDERAFSMDDRIAWTH 135 DKFZphtes3_2el2

group: Transcription Factors

DKFZphtes3_2el2 encodes a novel 849 ammo acid protein with similarity to Zmc finger proteins .

The new protein is a putative transcription factor with three C2H2 zmc fingers. Additionally, a cytochrome C family heme-binding site signature is present m the protein, which is only found in cytochrom C related proteins.

The new protein can find application m modulating/blocking the expression of genes controlled by this transcription factor. similarity to fmger proteins complete cDNA, complete eds, 5 EST hits

Sequenced by EMBL

Locus: unknown

Insert length: 3205 bp

Poly A stretch at pos. 3192, polyadenylation signal at pos. 3171

1 GGCACGGCCG GGTCCTGGCT GGCCAAACGA GGCTCGCGGA AGCAGCAGCC 51 GCCGCCTGAC CGCAGCTGGA TTTTGAAGAT TGATCCAAGG GACTGTATTA

101 ATTTCAGGAA TTGATTTGAA AGACACTGGC TCTGCCACTT AACAGCCATG

151 TAACCTTGGA TATGGAAGAA AGTAGCAGTG TTGCCATGTT GGTGCCAGAT

201 ATTGGGGAAC AGGAAGCTAT ACTGACTGCT GAAAGTATCA TCAGTCCTTC

251 ATTGGAAATT GATGAACAAA GAAAAACTAA ACCAGATCCA TTAATCCATG

301 TTATCCAGAA GTTAAGCAAG ATAGAAAAAT GAAAAGTCAC AAAAATGTCT

351 TTTAATTGGG AAGAAACGCC CACGTTCAAG TGCTGCAACA CACTCTCTTG

401 AAACCCAAGA ACTTTGTGAG ATTCCGGCTA AAGTAATCCA GTCACCTGCT

451 GCTGATACTA GAAGGGCTGA GATGTCACAA ACAAATTTTA CCCCTGACAC

501 TCTTGCCCAG AATGAAGGGA AGGCTATGTC TTATCAGTGT AGCCTTTGTA

551 AGTTTCTATC ATCATCCTTT TCCGTGTTAA AAGATCATAT TAAGCAACAT

601 GGTCAGCAAA ATGAAGTGAT ACTGATGTGC TCAGAGTGCC ATATTACATC

651 TAGAAGCCAG GAGGAACTTG AAGCCCACGT GGTGAATGAC CATGACAATG

701 ATGCCAATAT CCACACCCAA TCCAAAGCCC AACAGTGCGT AAGCCCCTCC

751 AGCTCTTTGT GTCGGAAAAC CACAGAAAGA AATGAAACCA TTCCAGATAT

801 CCCAGTAAGT GTGGACAATC TACAGACTCA TACTGTCCAA ACTGCATCTG

851 TGGCAGAAAT GGGTAGGAGG AAATGGTATG CATACGAACA GTACGGCATG

901 TATCGATGCT TGTTTTGTAG TTATACTTGT GGCCAGCAGA GAATGTTGAA

951 AACACACGCT TGGAAACATG CTGGGGAGGT TGATTGCTCC TATCCAATCT 1001 TTGAAAATGA AAATGAACCC CTAGGCCTGC TGGATTCTTC AGCAGCTGCT 1051 GCGCCTGGTG GGGTCGATGC AGTCGTCATT GCTATTGGAG AGAGTGAACT 1101 GAGTATCCAC AATGGGCCAT CAGTGCAAGT GCAGATTTGC AGCTCAGAAC 1151 AGTTATCATC TTCATCTCCT TTAGAACAGA GTGCAGAAAG AGGAGTACAC 1201 CTAAGTCAGT CAGTTACCCT GGACCCCAAT GAGGAAGAAA TGCTAGAAGT 1251 GATTTCTGAT GCAGAGGAGA ATCTGATTCC TGATAGCCTG CTTACATCAG 1301 CACAGAAAAT CATCAGCAGC AGCCCCAATA AAAAAGGGCA TGTTAACGTG 1351 ATAGTGGAGC GATTGCCAAG TGCTGAAGAA ACCCTTTCAC AGAAGCGCTT 1401 CCTCATGAAC ACTGAAATGG AAGAAGGGAA GGACCTGAGC CTGACAGAAG 1451 CTCAGATTGG GCGCGAAGGA ATGGATGATG TTTATCGTGC TGATAAATGT 1501 ACTGTTGATA TTGGGGGATT GATCATAGGC TGGAGCAGTT CAGAGAAAAA 1551 AGACGAGTTA ATGAATAAAG GCCTGGCTAC TGATGAGAAT GCCCCACCAG 1601 GCCGGAGAAG GACAAATTCT GAGTCTCTTC GATTACACTC ATTAGCTGCA 1651 GAAGCCCTTG TCACAATGCC TATAAGAGCT GCAGAGTTGA CAAGAGCCAA 1701 CCTGGGGCAC TATGGAGATA TAAACCTTTT AGATCCAGAT ACTAGTCAAA 1751 GGCAAGTAGA TAGTACATTG GCAGCGTACT CAAAAATGAT GTCGCCACTT 1801 AAAAACTCTT CAGATGGATT AACTAGTCTT AACCAAAGCA ACTCCACCTT 1851 GGTAGCACTC CCAGAGGGTA GGCAGGAATT GTCAGATGGG CAGGTTAAGA 1901 CAGGCATCAG CATGTCCTTA CTCACCGTCA TTGAAAAATT GAGAGAAAGG 1951 ACAGACCAAA ACGCTTCAGA CGATGACATT TTGAAAGAGT TGCAGGACAA 2001 CGCCCAGTGC CAACCCAACA GCGATACAAG TTTGTCCGGA AACAATGTGG 2051 TGGAATACAT CCCGAATGCT GAACGACCCT ACCGTTGCCG CCTGTGTCAC 2101 TACACAAGTG GCAACAAGGG CTACATCAAG CAGCACTTAC GAGTCCATCG 2151 ACAGAGACAG CCTTATCAGT GTCCTATCTG CGAGCACATA GCGGACAACA 2201 GCAAAGATTT GGAGAGTCAC ATGATCCACC ACTGTAAGAC AAGAATATAC 2251 CAGTGCAAGC AGTGTGAAGA ATCCTTCCAT TATAAGAGTC AATTGAGGAA 2301 CCATGAGAGA GAACAGCACA GTCTTCCAGA TACCTTGTCA ATAGCAACTT 2351 CTAATGAGCC AAGAATTTCC AGTGATACAG CTGATGGAAA ATGTGTCCAG 2401 GAAGGGAATA AGTCTTCAGT CCAGAAACAA TATAGATGTG ATGTGTGTGA 2451 TTATACAAGT ACAACATATG TTGGTGTCAG AAACCACAGG CGAATCCATA 2501 ACTCTGATAA GCCGTACAGA TGCTCTCTGT GTGGGTATGT GTGTAGCCAT 2551 CCTCCTTCTT TGAAGTCTCA TATGTGGAAA CATGCAAGTG ACCAAAATTA 2601 CAACTACGAA CAAGTAAACA AGGCTATTAA CGACGCGATT TCACAAAGTG

2651 GCAGAGTTCT GGGGAAATCC CCTGGAAAGA CTCAATTAAA GAGCAGTGAA

2701 GAGAGTGCAG ATCCCGTCAC TGGAAGTTCG GAAAATGCAG TGTCATCTTC

2751 AGAACTGATG TCCCAGACTC CCAGTGAAGT TCTGGGTACC AACGAGAATG

2801 AGAAACTGAG CCCTACAAGT AATACCTCAT ATAGTTTAGA AAAAATCTCC

2851 AGTCTGGCCC CTCCTAGCAT GGAGTACTGC GTTTTACTCT TCTGCTGTTG

2901 TATTTGTGGT TTTGAATCAA CCAGCAAAGA AAACCTCTTG GATCATATGA

2951 AAGAGCACGA GGGTGAAATT GTAAACATCA TCCTGAATAA GGACCACAAT

3001 ACAGCTCTAA ACACAAATTA GGTGGAATAA TGACTCGAGC AGGAAAGCAG

3051 TAGAAGAGGA TTCCTTCACC ACAGTTTCAC CTTTACGCTG TCAGACAACT

3101 TCCTGCCACA GAAGAAGTCG TTGATGTGAT TTTTGAGGAA ATGACAGATG

3151 TGACTTTGGA ACCAAACTTG TAATAAAAGG AATTCCAAAT GGAAAAAAAA

3201 AAAAA

BLAST Results

No BLAST result

Medlme entries

90301500:

Cloning and sequencing of a zmc finger cDNA expressed m mouse testis.

92310982:

Zfp-37, a new murine zinc finger encoding gene, is expressed in a developmentally regulated pattern in the male germ line.

Peptide information for frame 1

ORF from 472 bp to 3018 bp; peptide length: 849 Category: similarity to known protein

1 MSQTNFTPDT LAQNEGKAMS YQCSLCKFLS SSFSVLKDHI KQHGQQNEVI

51 LMCSECHITS RSQEELEAHV VNDHDNDANI HTQSKAQQCV SPSSSLCRKT

101 TERNETIPDI PVSVDNLQTH TVQTASVAEM GRRKWYAYEQ YGMYRCLFCS

151 YTCGQQRMLK THAWKHAGEV DCSYPIFENE NEPLGLLDSS AAAAPGGVDA

201 VVIAIGESEL SIHNGPSVQV QICSSEQLSS SSPLEQSAER GVHLSQSVTL

251 DPNEEEMLEV ISDAEENLIP DSLLTSAQKI ISSSPNKKGH VNVIVERLPS

301 AEETLSQKRF LMNTEMEEGK DLSLTEAQIG REGMDDVYRA DKCTVDIGGL

351 IIGWSSSEKK DELMNKGLAT DENAPPGRRR TNSESLRLHS LAAEALVTMP

401 IRAAELTRAN LGHYGDINLL DPDTSQRQVD STLAAYSKMM SPLKNSSDGL

451 TSLNQSNSTL VALPEGRQEL SDGQVKTGIS MSLLTVIEKL RERTDQNASD

501 DDILKELQDN AQCQPNSDTS LSGNNVVEYI PNAERPYRCR LCHYTSGNKG

551 YIKQHLRVHR QRQPYQCPIC EHIADNSKDL ESHMIHHCKT RIYQCKQCEE

601 SFHYKSQLRN HEREQHSLPD TLSIATΞNEP RISSDTADGK CVQEGNKSSV

651 QKQYRCDVCD YTSTTYVGVR NHRRIHNSDK PYRCSLCGYV CSHPPSLKSH

701 MWKHASDQNY NYEQVNKAIN DAISQSGRVL GKSPGKTQLK SSEESADPVT

751 GSSENAVSSS ELMSQTPSEV LGTNENEKLS PTSNTSYSLE KISSLAPPSM

801 EYCVLLFCCC ICGFESTSKE NLLDHMKEHE GEIVNIILNK DHNTALNTN

BLASTP hits

Entry S10245 from database PIR: finger protein, testis - mouse

Score = 265, P = 8.4e-23, identities = 61/205, positives = 91/205

Entry S22954 from database PIR: fmger protein zfp-37 - mouse

Score = 265, P = 9.1e-22, identities = 61/205, positives = 91/205

Entry AF031657_1 from database TREMBL: gene: "Zfp94"; product: "zmc-fmger protein 94"; Rattus norvegicus zinc-finger protein 94 (Zfp94) gene, partial eds.

Score = 243, P = 1.6e-21, identities = 57/190, positives = 85/190

Alert BLASTP hits for DKFZphtes3_2el2, frame 1 No Alert BLASTP hits found Pedant information for DKFZphtes3_2el2, frame 1

Report for DKFZphtes3_2el2.1

[LENGTH] 849

[MW] 94325. 42

[pi] 5.47

[HOMOL] PIR:A5 4661 zmc finger protein ZNF41 - human (fragment) 2e-22

[FUNCAT) 04.05. 01.04 transcriptional control [Ξ. cerevisiae, YJL056c] 3e-09

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YJL056c] 3e-09

[FUNCAT] 04.03. 01 trna synthesis [Ξ. cerevisiae, YPR186c PZF1 - TFIIIA] le-07

[FUNCAT] 04.01. 01 rrna synthesis [S. cerevisiae, YPR186c PZF1 - TFIIIA] le-07

[FUNCAT] 04.99 other transcription activities [S. cerevisiae, YOR113w] 4e-07

[FUNCAT] 01.05. 04 regulation of carbohydrate utilization [S. cerevisiae, YGL209w]

2e-04

[FUNCAT] 13.04 homeostasis of other ions [S. cerevisiae, YNL027w] 2e-04

[FUNCAT] 11.01 stress response [S. cerevisiae, YMR037c] 3e-04

[BLOCKS] BL00028 Zinc finger, C2H2 type, domain proteins

[SCOP] dlmeyg_ 9.6.1.1.1 a designed zinc fmger protein [syntheti 8e-06

[PIRKW] nucleus 8e-18

[PIRKW] RNA binding 5e-13

[PIRKW] duplication 7e-13

[PIRKW] tandem repeat le-21

[PIRKW] spermatogenesis 6e-16

[PIRKW] zinc 9e-21

[PIRKW] zinc finger le-21

[PIRKW] DNA binding le-21

[PIRKW] metal binding 3e-15

[PIRKW] phosphoprotein 5e-13

[PIRKW] leucine zipper le-13

[PIRKW] alternative splicing 6e-18

[PIRKW] eye lens 2e-16

[PIRKW] oocyte le-12

[PIRKW] transcription factor 6e-18

[PIRKW] segmentation 7e-13

[PIRKW] embryo le-12

[PIRKW] transcription regulation 2e-19

[PIRKW] homeobox 2e-08

[SUPFAM] POZ domain homology 7e-15

[SUPFAM] transcription factor Krueppel 7e-13

[SUPFAM] z c finger protein ZFP-36 le-21

[SUPFAM] homeobox homology 2e-08

[SUPFAM] unassigned homeobox proteins 2e-08

[PROSITE] CYTOCHROME_C 1

[PROSITE] MYRISTYL 10

[PROSITE] ZINC_FINGER_C2H2 3

[PROSITE] AMIDATION 2

[PROSITE] CAMP_PHOSPHO_SITE 2

[PROSITE] CK2_PHOSPHO_SITE 18

[PROSITE] TYR_PHOSPHO_SITE 3

[PROSITE] PKC_PHOSPHO_SITE 10

[PROSITE] ASN_GLYCOSYLATI0N 7

[PFAM] Zmc finger, C2H2 type

[KW] Irregular

[KW] 3D

[KW] LOW COMPLEXITY 5.65 %

SEQ MSQTNFTPDTLAQNEGKAMSYQCSLCKFLSSSFSVLKDHIKQHGQQNEVILMCSECHITS SEG xxxxxxxxxxxxxxx lmeyF

SEQ RSQEELEAHVVNDHDNDANIHTQSKAQQCVSPSSSLCRKTTERNETIPDIPVSVDNLQTH SEG lmeyF

SEQ TVQTASVAEMGRRKWYAYEQYGMYRCLFCSYTCGQQRMLKTHAWKHAGEVDCSYPIFENE SEG lmeyF

SEQ NEPLGLLDΞSAAAAPGGVDAVVIAIGESELSIHNGPSVQVQICSSEQLSSSSPLEQSAER SEG xxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxx... lmeyF

SEQ GVHLSQSVTLDPNEEEMLEVISDAEENLIPDSLLTSAQKIIΞSSPNKKGHVNVIVERLPS SEG lmeyF SEQ AEETLSQKRFLMNTEMEEGKDLSLTEAQIGREGMDDVYRADKCTVDIGGLIIGWSSSEKK SEG lmeyF

SEQ DELMNKGLATDENAPPGRRRTNSESLRLHSLAAEALVTMPIRAAELTRANLGHYGDINLL SEG lmeyF

SEQ DPDTSQRQVDSTLAAYSKMMSPLKNΞSDGLTSLNQSNSTLVALPEGRQELSDGQVKTGIS SEG lmeyF

SEQ MSLLTVIEKLRERTDQNASDDDILKELQDNAQCQPNSDTΞLSGNNVVEYIPNAERPYRCR SEG lmeyF TTTEETT

SEQ LCHYTSGNKGYIKQHLRVHRQRQPYQCPICEHIADNSKDLESHMIHHCKTRIYQCKQCEE SEG lmeyF TTTCEETTHHHHHHHHHHHHTTCCEEETTTTEEECCHHHHHHHHHHHHCCCCEEETTTTE

SEQ SFHYKΞQLRNHEREQHSLPDTLSIATSNEPRISSDTADGKCVQEGNKSSVQKQYRCDVCD SEG lmeyF EECCHHHHHHHHHHHC

SEQ YTSTTYVGVRNHRRIHNSDKPYRCSLCGYVCSHPPSLKSHMWKHASDQNYNYEQVNKAIN SEG lmeyF

SEQ DAISQSGRVLGKSPGKTQLKSSEESADPVTGSSENAVSΞSELMSQTPSEVLGTNENEKLS SEG lmeyF

SEQ PTSNTSYSLEKISSLAPPSMEYCVLLFCCCICGFESTSKENLLDHMKEHEGEIVNIILNK SEG lmeyF

SEQ DHNTALNTN SEG lmeyF

Prosite for DKFZphtes3_2el2.1

PS00001 104->108 ASN_GLYCOSYLATION PDOC00001 PS00001 445->449 ASN_GLYCOSYLATION PDOC00001 PS00001 454->458 ASN_GLYCOSYLATION PDOC00001 PS00001 457->461 ASN_GLYCOSYLATION PDOC00001 PS00001 497->501 ASN_GLYCOSYLATION PDOC00001 PS00001 646->650 ASN_GLYCOSYLATION PDOC00001 PS00001 784->788 ASN_GLYCOSYLATION PDOC00001 PS00004 98->102 CAMP_PHOSPHO_SITE PDOC00004 PS00004 378->382 CAMP_PHOSPHO_SITE PDOC00004 PS00005 59->62 PKC_PHOSPHO_SITE PDOC00005 PS00005 101->104 PKC_PHOSPHO_SITE PDOC00005 PS00005 306->309 PKC_PHOΞPHO_SITE PDOC00005 PS00005 357->360 PKC_PHOSPHO_SITE PDOC00005 PS00005 385->388 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 425->428 PKC_PHOSPHO_SITE PDOC00005 PS00005 678->681 PKC_PHOSPHO_SITE PDOC00005 PS00005 696->699 PKC_PHOSPHO_SITE PDOC00005 PS00005 726->729 PKC_PHOSPHO_SITE PDOC00005 PS00005 817->820 PKC_PHOSPHO_SITE PDOC00005 PS00006 62->66 CK2_PHOSPHO_SITE PDOC00006 PS00006 106->110 CK2_PHOSPHO_SITE PDOC00006 PS00006 126->130 CK2_PHOSPHO_SITE PDOC00006 PS00006 232->236 CK2_PHOSPHO_SITE PDOC00006 PS00006 262->266 CK2_PHOSPHO_SITE PDOC00006 PS00006 300->304 CK2_PHOSPHO_SITE PDOC00006 PS00006 314->318 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 323->327 CK2_PHOSPHO_SITE PDOC00006 PS00006 355->359 CK2_PHOSPHO_SITE PDOC00006 PS00006 381->385 CK2_PHOSPHO_SITE PDOC00006 PS00006 485->489 CK2_PHOSPHO_SITE PDOC00006 PS00006 499->503 CK2_PHOSPHO_SITE PDOC00006 PS00006 617->621 CK2_PHOSPHO_SITE PDOC00006 PS00006 626->630 CK2_PHOSPHO_SITE PDOC00006 PS00006 741->745 CK2_PHOSPHO_SITE PDOC00006 PS00006 758->762 CK2_PHOSPHO_SITE PDOC00006 PS00006 766->770 CK2_PHOSPHO_SITE PDOC00006 PS00006 817->821 CK2 PHOSPHO SITE PDOC00006 PS00007 331->339 TYR_PHOSPHO_SITE PDOC00007 PS00007 703->711 TYR_PHOSPHO_SITE PDOC00007 PS00007 596->605 TYR_PHOSPHO_SITE PDOC00007 PS00008 142->148 MYRISTYL PDOC00008 PS00008 185->191 MYRISTYL PDOC00008 PS00008 196->202 MYRISTYL PDOC00008 PS00008 241->247 MYRISTYL PDOC00008 PS00008 349->355 MYRISTYL PDOC00008 PΞ00008 473->479 MYRISTYL PDOC00008 PS00008 478->484 MYRISTYL PDOC00008 PS00008 645->651 MYRISTYL PDOC00008 PΞ00008 751->757 MYRISTYL PDOC00008 PS00008 772->778 MYRISTYL PDOC00008 PS00009 130->134 AMIDATION PDOC00009 PS00009 376->380 AMIDATION PDOC00009 PΞ00028 146->167 ZINC_FINGER_C2H2 PDOC00028 PS00028 684->705 ZINC_FINGER_C2H2 PDOC00028 PS00028 595->617 ZINC_FINGER_C2H2 PDOC00028 PS00190 53->59 CYTOCHROME C PDOC00169

Pfam for DKFZphtes3_2el2.1

HMM_NAME Z c finger, C2H2 type

HMM *CpwPDCgKtFrrwsNLrRHMR.T.H* C++ C+ T R++++L++H H

Query 53 CSE—CHITSRSQEELEAHVVN-DH 74

23.25 (bits) f: 539 t: 559 Target: dkfzphtes3_2el2.1 similarity to fmger proteins

Alignment to HMM consensus: Query *CpwPDCgKtFrrwsNLrRHMRTH*

C C++T ++ ++H+R+H dkfzphtes3 539 CRL—CHYTSGNKGYIKQHLRVH 559

Query f: 567 t: 587 Target: dkfzphtes3_2el2.1 similarity to finger proteins

Alignment to HMM consensus : HMM *CpwPDCgKtFrrwsNLrRHMRTH*

CP+ C+ ++ +L+ HM+ H Query 567 CPI—CEHIADNSKDLESHMIHH 587

33.47 (bits) f: 595 t: 616 Target: dkfzphtes3_2el2.1 similarity to fmger proteins

Alignment to HMM consensus: Query *CpwPDCgKtFrrwsNLrRHMR.T.H*

C+ C+++F ++Ξ+LR+H R H dkfzphtes3 595 CKQ—CEESFHYKSQLRNHERE-QH 616

Query f: 656 t: 676 Target: dkfzphtes3_2el2.1 similarity to fmger proteins

Alignment to HMM consensus: HMM *CpwPDCgKtFrrwsNLrRHMRTH*

C++ C++T ++ R+H+R+H Query 656 CDV—CDYTSTTYVGVRNHRRIH 676

24.53 (bits) f: 684 t: 704 Target: dkfzphtes3_2el2.1 similarity to finger proteins

Alignment to HMM consensus: Query *CpwPDCgKtFrrwsNLrRHMRTH*

C+ CG++ +++ +L+ HM H dkfzphtes3 684 CΞL--CGYVCSHPPSLKSHMWKH 704

Query f: 809 t: 829 Target: dkfzphtes3_2el2.1 similarity to fmger proteins

Alignment to HMM consensus: HMM *CpwPDCgKtFrrwsNLrRHMRTH*

C + CG ++++NL HM+ H Query 809 CCI--CGFESTSKENLLDHMKEH 829 DKFZphtes3_2fl4

group: testes derived

DKFZphtes3_2f14 encodes a novel 129 amino acid protein with very weak similarity to human omega protein.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . weak similarity to omega protein complete cDNA, complete eds, 1 EST hit

Sequenced by EMBL

Locus: unknown

Insert length: 2353 bp

Poly A stretch at pos. 2341, no polyadenylation signal found

1 GCAGATTCTC CAGGCCCAGC ATCTGCCTCA CCGTGGCCCC CCACAAGCCA 51 AGCGCCTGCC TTTCAGCAGC CTCTACACAC CCAGCTCCTG CCACCCAATG

101 GCTCTTTAGG CCAAGCTCAT ACCTCACGAT GATTTTTCCA GGCCCAACTT

151 TTGTCTCATG GCAACCTTCC CTGGCCAAGT TTCCACCTAT TTCCTGGCAG

201 CCTGGACAGG CCCAGGTCCT GCCACACACT GGCCTCTCTA CGCCCAGCTC

251 ATGCCTCACA GTGGCCTCTC CAGGCCCAGC TCCTGTCCCG GGACATCATC

301 TCCAGGCCCA AAACTTCCTC AAGTCGGCCT CTCCAGGCCC AGTTGCTGCC

351 TCCCGGCATT CTCTCCAGGC CTAGCTCTTC CTCCTGGCTG TATCTACAAG

401 ACCAACTCCT GCCTCACAAC AACCTTTTAT GGCTCAGCTC CTGCCCAACT

451 ACTGCCGGCC TTTGTAGGCC CAAAACTTCC TCAAGTCAAG CTCTTTAGGC

501 CCACCTTCTG CCTTGCAGTG GCCTGTACAG ACCCAGCTCT GGCTTGAGAA

551 CAGCCTCTGC AGGCCCTGCT CTTGCCTCTT AGCTCCCTCT CCAGGCCCAT

601 CTCTTGCCTC ACAGTGGCTT CCGTGGGCCA AGTTCCCGCC TGCCTCCCAG

651 CAGCCTCAAC AGGCCTAGCT CCTCCCTCAC AATGGCTTGT TTAGGTCCAG

701 TTGATGCCTC TGGCAACCTG TCCAGGCCCA GCTCCTGCCT CACACTGGCC

751 TCTCTAGGCC GAGGTCCTTT CTCATACTGG CCTGTTTAGG CCCAGCTCAT

801 TCCTCTTGTC ATCTCTCCAG GCCCAGCTTT TGCCTGTTGT TGGCCTCTAC

851 CTCACAGTGC ACCTTCCAGT CCCACCTCTT GCCTCACCAT GGCCTCCTCT

901 GACCAGGTTC CTGCCTTTCG GCAGCCTCTA CAGGCCTAGC TGCTGCCTCC

951 CAATGGCCTT TGTAGGCCAC GCTCATGCCT CACTGTGGCC TTTCCAGGCC 1001 TAGCTTTCGC TTTTTGGCCA CTCCAGGCCC AGAACTTCCC CCAGTCAGCC 1051 TCTCCAGGCC CAGCTCTTCC TCCCAGCAAC CTCTGCAGGC CCAAATCATC 1101 CTCAAATTGG CCTCTTCTTT CCCAGCTCCT GCCTCCTGGT GGCCTCTGAA 1151 GACCCAAATC GTCCTCCAGT TGGTTTTTCC AGGCCCAGCT CCTGCCTTTT 1201 GGTGGCCTCT CCAGGTGCAA AACTTCCTCC CATCAGCCTG TCCAGGCCCA 1251 GCTCATGCCT CTTGGTGGCC TTCTCAGGCC CTGCTTTTGA CTTGGTGGCC 1301 TCTTCAGGCC CAGAACTTGA ACTCAAGTCA GCCTCTCCAG GCCCAGCTCC 1351 TGCCTTCTTA AGGTCTGTAC AGGCCCAGCC TCTACCTCAC AGCGGACTCT 1401 CCACACCCAG CTCTTGCCTC ACTGTAGCCT CCCCAGTCCA AAACTCCTGC 1451 CTTTTGGCAG CTTCGACAAG CCCAGCTCCT GCCTTTCAAT GACCTCTTTA 1501 GGCCCCGCTC ATTCCTTACA ACGGCCTTTC CAGGCCCAGT TTTTCCCTTT 1551 TGGCGGCCTC TCCAGGCCCA GAACTTCCTC AAGTCGGCCT CTTTAGGCCC 1601 AGTTGCTGCC TCCTGGCATC CTCTGCAGGC CGAGCTCTTC CTCCCTGCTG 1651 TGTCTACAGG CCCAACTCCT GCCTCACAAC AACCTCCTTG GACTCAGCTT 1701 CTGCCCAGCT CCTGGTGGCC TTTGTAGGCT CAAAATTTTC TCAAATCAAG 1751 CTCTCCAGGC CTACTGTCAG CCTCGTGGCA GCCTAAACAG GCCCAGCTCC 1801 TGCCTGACAA TGGCCTCTCC AGGCTTTTCT CCTGCCTCGC AGCAGGCTTT 1851 CCAGGCCCAG CTCTTGCCTC ATGGTGGCCT TCCCCGGCCA TGTTCCTATC 1901 TGACTTCTGG CAGCCTCAAC CGGCCCAGCT TCTGCCTCAC ACTGGCCTCT 1951 CTAGGCCCAG CTCCTTTTTC ACAGTGGCCT CACTACGCCC ATCTCCTACC 2001 TCAGATCTGC CTCCCAAGAC CCAGCTCCTG TCTCATGGTG GTCTCTCTTA 2051 CACCAGCTCC TGCCTCACAA TGGCCTCGTC TGGCCCATCT TCTGCCTCAC 2101 AGTGGCCACT CAAGGCCCAT CTTTTGCCTC ATGGTAGCCT CTTCTGGTTT 2151 TGCTCTTGCC TCACAGTTGC CTCTTCCAGA TCCAGCTTTA AGCCTTTGAT 2201 GGTCAACAGC ATCAAGGAGC CTAAAGCTTC CCTGGACTCT CATTTGTTCA 2251 CTTTACAGCA GAGTGCCTTA GCAAAAACTG TCTCTTAACC TTGAGAGTGG 2301 ATTTCTGACA AATCGATAGT AAATTCTGCC TGTGTGGTTT CAAAAAAAAA 2351 AAA

BLAST Results

No BLAST result Medlme entries

No Medline entry

Peptide information for frame 2

ORF from 158 bp to 544 bp; peptide length: 129 Category: similarity to known protein

1 MATFPGQVST YFLAAWTGPG PATHWPLYAQ LMPHSGLSRP SSCPGTSSPG 51 PKLPQVGLSR PSCCLPAFSP GLALPPGCIY KTNSCLTTTF YGSAPAQLLP 101 AFVGPKLPQV KLFRPTFCLA VACTDPALA

BLASTP hits

Entry 170697 from database PIR: omega protein - human (fragment)

Score = 79, P = 2.8e-03, identities = 32/94, positives = 38/94

Alert BLASTP hits for DKFZphtes3_2f14, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphtes3_2f14, frame 2

Report for DKFZphtes3_2f1 .2

[LENGTH] 129

[MW] 13421.76

[pi] 9.14

[PROSITE] MYRISTYL 2

[KW] Irregular

[KW] LOW COMPLEXITY 10.85 %

SEQ MATFPGQVSTYFLAAWTGPGPATHWPLYAQLMPHSGLSRPSSCPGTSSPGPKLPQVGLSR

SEG xxxxxxxxxxxxxx

PRD cccccccceeehhhhhcccccccccccccccccccccccccccccccccccccccccccc

SEQ PSCCLPAFSPGLALPPGCIYKTNSCLTTTFYGSAPAQLLPAFVGPKLPQVKLFRPTFCLA

SEG

PRD cccccccccccccccccccccccccceeeccccccccccccccccccccccccccccccc

SEQ VACTDPALA

SEG

PRD ccccccccc

Prosite for DKFZphtes3_2f14.2

PS00008 6->12 MYRISTYL PDOC00008

PS00008 92->98 MYRISTYL PDOC00008

(No Pfam data available for DKFZphtes3_2f14.2 ) DKFZphtes3_2g7

group: testes derived

DKFZphtes3_2g7 encodes a novel 359 ammo acid protein with similarity to neurofiliament proteins .

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . similarity to neurofilament proteins complete cDNA, complete eds, 6 EST hits (5 hits are out of a testis library)

Sequenced by EMBL

Locus: unknown

Insert length: 1613 bp

Poly A stretch at pos. 1595, polyadenylation signal at pos. 1557

1 GCCACACAGG CTCCTTGGAG TAAGAGTGTG AGAAACTGGA TGAAGACAGC

51 TGTATTCTTT TGGAAGCGTT CGAGATTGGT CTGTCTCTAC CAACTAAAAA

101 CTTCTAGCTT AAGTGCAGAG ATTTAAGGAG ATCAACAAAA ACTCAGTCTA

151 GACATATTAT GAGGCTGGGA GGGTATCAAC AGACTTGAGT TCTTGTCAGC

201 AAGATCACCT GCTTTTAATA TTGTCCTCAG GGTCTGAGCA CATCTGGAAG

251 TGAGGTCAAT CAAGTTAGAC CCCAAAAACT TTTGTGACAA CAGTGAAGAG

301 GGGAAAATAA ACACACCACA AACATGAACC TCAACCCCCC GACATCTGCT

351 CTTCAGATCG AGGGCAAAGG CAGCCATATT ATGGCTAGAA ATGTAAGCTG

401 CTTTCTAGTC AGGCACACCC CTCATCCCAG AAGAGTCTGC CACATCAAAG

451 GCTTGAATAA CATTCCAATC TGTACTGTGA ATGATGATGA GAATGCATTT

501 GGAACATTGT GGGAAGTTGG CCAGTCTAAC TACTTAGAGA AGAACAGGAT

551 ACCATTTGCC AATTGCAGTT ACCCCCCGAG CACTGCAGTC CAGAAGAGCC

601 CTGTAAGAGG AATGTCGCCA GCCCCAAACG GTGCCAAAGT GCCTCCACGG

651 CCTCATTCTG AGCCCAGTAG AAAAATTAAA GAGTGCTTCA AAACTTCCAG

701 TGAGAATCCC TTAGTAATTA AAAAGGAAGA AATTAAGGCC AAAAGACCAC

751 CATCACCTCC AAAGGCATGC TCTACTCCTG GCTCCTGTTC TTCAGGGATG

801 ACAAGTACCA AGAATGATGT GAAAGCAAAC ACCATTTGCA TACCAAACTA

851 TCTGGATCAG GAAATAAAAA TCCTGGCAAA GCTCTGTAGC ATTTTGCATA

901 CTGATTCTCT GGCAGAAGTT TTACAGTGGC TGCTTCATGC AACTTCAAAA

951 GAAAAAGAGT GGGTCTCAGC TTTGATTCAT TCTGAGCTTG CCGAGATAAA

1001 CCTGTTAACT CATCACAGAA GAAACACCTC AATGGAACCA GCAGCAGAGA

1051 CTGGGAAGCC ACCCACAGTT AAATCACCAC CCACAGTTAA ATTGCCCCCA

1101 AATTTTACTG CAAAATCAAA AGTGCTGACC AGAGATACAG AAGGGGATCA

1151 ACCAACCAGA GTGTCAAGTC AAGGATCTGA AGAAAACAAG GAAGTACCAA

1201 AAGAGGCTGA GCACAAGCCT CCACTACTTA TAAGAAGAAA TAATATGAAA

1251 ATACCTGTTG CAGAATATTT CAGCAAACCA AATTCTCCTC CCAGGCCTAA

1301 CACTCAGGAG AGTGGATCAG CAAAACCAGT GTCAGCAAGG AGTATACAAG

1351 AATACAACCT CTGTCCCCAA AGAGCATGTT ATCCTTCAAC ACACCGGAGG

1401 TAGAAGTTCT AGACTGGGTG AATTCTTTCA TGAATATGAG CTTCACATTT

1451 ACATCATCAA ATTATTTTTC AAATGAATAT TTTTGGTATT GAGGAATCAA

1501 GTGGTCCTCT TTATGGTGGC ACATGTAAAT CTAAAAATAC CTGTATGTAA

1551 TGCTACAAAT AAATATTACT GGAAATGATA TTTCCATTTG TAGTTAAAAA

1601 AAAAAAAAAA AAA

BLAST Results

No BLAST result

Medline entries

No Medlme entry

Peptide information for frame 3

ORF from 324 bp to 1400 bp; peptide length: 359 Category: similarity to known protein 1 MNLNPPTSAL QIEGKGSHIM ARNVSCFLVR HTPHPRRVCH IKGLNNIPIC

51 TVNDDENAFG TLWEVGQSNY LEKNRIPFAN CSYPPSTAVQ KSPVRGMSPA

101 PNGAKVPPRP HSEPSRKIKE CFKTSSENPL VIKKEEIKAK RPPSPPKACS

151 TPGSCSSGMT STKNDVKANT ICIPNYLDQE IKILAKLCSI LHTDSLAEVL

201 QWLLHATSKE KEWVSALIHS ELAEINLLTH HRRNTSMEPA AETGKPPTVK

251 SPPTVKLPPN FTAKSKVLTR DTEGDQPTRV SSQGSEENKE VPKEAEHKPP

301 LLIRRNNMKI PVAEYFSKPN SPPRPNTQES GSAKPVSARS IQEYNLCPQR

351 ACYPSTHRR

BLASTP hits

Entry A43427 from database PIR: neurofilament triplet HI protein - rabbit (fragment)

Score = 118, P = 5.6e-04, identities = 79/290, positives = 110/290

Entry RNNFH_1 from database TREMBL:

Rat high molecular weight neurofilament (NF-H) protein mRNA, 3' end.

Score = 115, P = 9.5e-04, identities = 69/281, positives = 100/281

Entry B43427 from database PIR: neurofilament protein H form H2 (repetitive region) - rabbit (fragment)

Score = 111, P = 1.3e-03, identities = 64/269, positives = 102/269

Alert BLASTP hits for DKFZphtes3_2g7, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphtes3_2g7, frame 3

Report for DKFZphtes3_2g7.3

[LENGTH] 359

[MW] 39725.53

[pi] 9.45

[PROSITE] MYRISTYL 3

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 9

[PROSITE] PKC_PHOSPHO_SITE 10

[PROSITE] ASN_GLYCOSYLATION 4

[KW] Alpha_Beta

[KW] LOW_COMPLEXITY 4.18 %

SEQ MNLNPPTSALQIEGKGSHIMARNVSCFLVRHTPHPRRVCHIKGLNNIPICTVNDDENAFG SEG PRD ccccccccceeecccccceeeeccceeeeecccccccccccccccccccccccccccccc

SEQ TLWEVGQSNYLEKNRIPFANCSYPPSTAVQKSPVRGMSPAPNGAKVPPRPHSEPSRKIKE SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccchhhhhh

SEQ CFKTSSENPLVIKKEEIKAKRPPSPPKACSTPGSCSSGMTSTKNDVKANTICIPNYLDQE SEG PRD hcccccccceeeehhhhhhccccccccccccccccccccccccccccceeeeccccchhh

SEQ IKILAKLCSILHTDSLAEVLQWLLHATSKEKEWVSALIHSELAEINLLTHHRRNTSMEPA SEG PRD hhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccc

SEQ AETGKPPTVKSPPTVKLPPNFTAKSKVLTRDTEGDQPTRVSSQGSEENKEVPKEAEHKPP SEG .... xxxxxxxxxxxxxx

PRD cccccccccccccccccccccccceeeeecccccccceeeeccccccccccccccccccc

SEQ LLIRRNNMKIPVAEYFSKPNSPPRPNTQESGSAKPVSARSIQEYNLCPQRACYPΞTHRR

SEG PRD eeeeccccccceeeeecccccccccccccccccccchhhhhhccccccccccccccccc

Prosite for DKFZphtes3_2g7.3

PS00001 23->27 ASN_GLYCOSYLATION PDOC00001 PS00001 80->84 ASN_GLYCOSYLATION PDOC00001 PS00001 234->238 ASN GLYCOSYLATION PDOC00001

^1 σ D α σ o α σ α o σ o a a D σ σ σ

O o o o o o O o o o O o o o o o o o o o o o o o

DKFZphtes3 2hl

group: transmembrane protein

DKFZphtes3_2hl encodes a novel 116 amino acid protein with weak similarity to C. elegans cosmid C13F10.

The novel protein contains 1 transmembrane region.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes and as a new marker for testicular cells. similarity to C. elegans C13F10.5

TRANSMEMBRANE 1

Sequenced by EMBL

Locus: /map="2"

Insert length: 1156 bp

Poly A stretch at pos. 1143, polyadenylation signal at pos. 1121

1 GGCCATCAAA ATAACTAAAC CATGTCATTT GGAGCAACAA AGCCACTGCG 51 GCCTCCATTT GGGCCAAGCT CTGACTGCAA TGATGCCTCT GCCCCGACCC

101 GGGCCTCGCT GTGACTGACA ATGCCGCTGC ATCTTTTCAG CAGTCATTGA

151 TGAGGAAGTA TCTACATCCT CCTTCCCACT ACCAGATTTT GCTTGGAGAA

201 AAGCAGTTTC CTGAAATAAT TCTGTGACGA GCTTCTTCCA CATTAGGACA

251 AAAATGCTGG AAGCGGCTCA GCCCCAGGGC AGCACATCAG AGACACCATG

301 GAACACAGCC ATTCCTCTGC CGTCGTGCTG GGACCAGTCT TTCCTGACCA

351 ATATCACCTT CTTGAAGGTT CTTCTCTGGT TGGTCCTGCT GGGACTGTTT

401 GTGGAACTGG AATTTGGCCT GGCATATTTT GTCCTGTCCT TGTTCTATTG

451 GATGTACGTC GGGACACGAG GCCCTGAAGA GAAGAAAGAG GGAGAGAAGA

501 GCGCCTACTC TGTGTTCAAT CCAGGCTGTG AAGCCATCCA GGGCACCCTG

551 ACTGCAGAGC AGTTGGAGCG CGAGTTACAG TTGAGACCCC TGGCAGGGAG

601 ATAGGACCCA GCTGTGCTGT CATGCAGCTA ACCTCTGATG TGGTCTTCCT

651 CACCATTGGC TATGGATTTG ATTTCAGGTG TATAGGACTA AGGGCAGCTT

701 GCGGGTTAGC TCTGTGACTG CATAGTTTTT CTACCTTCTT TCCCTGATCT

751 TTTGCTGCCA TTTGATCTTT GATAGTTTTG GTGAAACTCT CTAAAATACA

801 TTCACTGTGG GTCCGACGCA ATTTATAAAA ATTATGTACT CAAGAAGGGA

851 GACCTGTTTG TTTCATTTCT CATCTGTTTG GGAGATGATT TTAGAGCACT

901 AGAAAGGCAC TGGGGAGATT CTCAGCTTAA AACATCCAGC AGTTTGAAGT

951 ATGATTAGGT ACATCAGGGC TGCATTGTCA ATGTTCTCTT TAAGTCTTTT 1001 AACATTTATA GCAATTTTTT TTTTCCCGGA GAGTTTAGGT TGCAAGTTTT 1051 GGGTTTCTTG TTTGTTTTTG TTTTGCTTCC TGCTTTAATT CTTTAATTTT 1101 CAGTCATTAC TGGTATTGAA AAATAAAATA TCTTTAAAAC ATCAAAAAAA 1151 AAAAAA

BLAST Results

Entry HS313307 from database EMBL: human STS SHGC-16715. Score = 1222, P = 1.4e-48, identities = 248/251

Medline entries

No Medlme entry

Peptide information for frame 2

ORF from 254 bp to 601 bp; peptide length: 116 Category: similarity to unknown protein

1 MLEAAQPQGS TSETPWNTAI PLPSCWDQSF LTNITFLKVL LWLVLLGLFV 51 ELEFGLAYFV LSLFYWMYVG TRGPEEKKEG EKSAYSVFNP GCEAIQGTLT 101 AEQLERELQL RPLAGR BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_2hl, frame 2

TREMBL :CEUC13F10_2 gene: "C13F10.5"; Caenorhabditis elegans cosmid C13F10., N = 1, Score = 141, P = 8.2e-10

>TREMBL:CEUC13F10_2 gene: "C13F10.5"; Caenorhabditis elegans cosmid C13F10.

Length = 171

HSPs:

Score = 141 (21.2 bits), Expect = 8.2e-10, P = 8.2e-10 Identities = 32/82 (39%), Positives = 52/82 (63%)

Query: 27 DQSFLTNITFLKVLLWLVLLGLFVELEFGLAYFVLSLFYWMYVGTRGPEEKKEGEKSAYS 86

+QS ++ T + V++++V L ++FG +F+LSL + Y T G ++ GE SAYS Sbjct: 90 EQSVVS—TRIAVVVYVVGQALAAWVQFGAVFFILSLILFTYWNT-G—RRRRGEMSAYS 144

Query: 87 VFNPGCEAIQGTLTAEQLEREL 108

VFN CE + G++TAE ER++ Sbjct: 145 VFNDNCERLAGSMTAEHFERDM 166

Pedant information for DKFZphtes3_2hl, frame 2 Report for DKFZphtes3_2hl .2

[LENGTH] 116

[MW] 13092.19

[pi] 4.64

[PROSITE] MYRISTYL 1

[PROSITE] CK2 PHOSPHO SITE 2

[PROSITE] TYR PHOSPHO SITE 2

[PROSITE] ASN GLYCOSYLATION 1

[KW] TRANSMEMBRANE 1

[KW] LOW COMPLEXITY 32 .76 %

SEQ MLEAAQPQGSTSETPWNTAIPLPSCWDQSFLTNITFLKVLLWLVLLGLFVELEFGLAYFV

SEG xxxxxxxxxxxxxxxxxxxxx ....

PRD ccccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhchhhhh

MEM MMMMMMMMMMMMMMMMM

SEQ LSLFYWMYVGTRGPEEKKEGEKSAYSVFNPGCEAIQGTLTAEQLERELQLRPLAGR

SEG xxxxxxxxxxxxxxxxx ..

PRD hhhhhhhhcccccchhhhhcccceeeecccccccccccchhhhhhhhhhccccccc

MEM

Prosite for DKFZphtes3_2hl .2

PS00001 33->37 ASN_GLYCOSYLATION PDOC00001

PS00006 10->14 CK2_PHOSPHO_SITE PDOC00006

PS00006 24->28 CK2_PHOSPHO_SITE PDOC00006

PS00007 78->86 TYR_PHOSPHO_SITE PDOC00007

PS00007 77->86 TYR_PHOSPHO_SITE PDOC00007

PS00008 97->103 MYRISTYL PDOC00008

(No Pfam data available for DKFZphtes3 2hl.2) DKFZphtes3_2hl5

group: testes derived

DKFZphtes3_2hl5 encodes a novel 855 amino acid protein with very weak similarity to S. pombe cdc23.

No informative BLAST results; no predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . similarity to cdc23 complete cDNA, complete eds, EST hits

Sequenced by EMBL

Locus : unknown

Insert length: 4619 bp

Poly A stretch at pos. 4598, polyadenylation signal at pos. 4589

1 GAAGGCGTCC CGGCATCGGC CAAGATTCTA CATTGCTCAT CTGGGCATCT 51 GAGCCTCCTT CGAAGTTTCC TGTCACAACT GTCCTCTTGA CAGCATGGAT

101 GAGGAGGAAG ACAATCTGTC TCTGCTGACC GCACTGCTGG AAGAAAATGA

151 GTCAGCCTTG GATTGTAATT CAGAAGAAAA TAACTTCTTG ACGCGGGAAA

201 ATGGCGAGCC CGACGCATTT GATGAGCTCT TTGATGCCGA CGGCGACGGT

251 GAATCTTATA CAGAAGAGGC TGATGATGGA GAAACAGGAG AGACAAGAGA

301 CGAAAAGGAA AATCTGGCCA CTCTCTTTGG AGATATGGAG GACTTAACAG

351 ATGAAGAAGA AGTTCCCGCA TCACAGTCAA CTGAAAATAG GGTCCTCCCT

401 GCTCCTGCCC CCAGGCGAGA GAAAACGAAT GAAGAGTTGC AAGAGGAATT

451 AAGGAATTTG CAAGAGCAAA TGAAGGCCTT ACAAGAGCAG CTAAAAGTAA

501 CAACAATTAA ACAGACAGCA AGCCCAGCCC GTCTGCAAAA ATCCCCTGAG

551 AAGTCTCCCC GGCCACCTCT TAAGGAGAGG AGAGTTCAGA GAATTCAGGA

601 GTCAACATGC TTTTCTGCGG AGCTTGATGT CCCTGCGCTA CCAAGAACCA

651 AGAGGGTGGC TCGAACACCA AAGCCTTCAC CTCCAGATCC CAAAAGCTCA

701 TCTTCAAGGA TGACAAGTGC ACCCTCCCAA CCCCTACAGA CGATTTCTCG

751 GAACAAACCT AGTGGGATAA CTAGAGGTCA AATTGTGGGG ACCCCAGGAA

801 GTTCTGGGGA AACGACTCAA CCCATCTGTG TGGAAGCCTT CTCTGGTCTG

851 CGGCTCAGGC GGCCTCGAGT ATCCTCCACA GAAATGAACA AGAAAATGAC

901 CGGCCGAAAA CTGATCAGAC TGTCTCAGAT CAAGGAAAAG ATGGCCAGAG

951 AGAAGCTGGA AGAAATAGAT TGGGTGACAT TTGGGGTTAT ATTGAAGAAG 1001 GTTACGCCAC AGAGTGTGAA TAGTGGAAAA ACCTTCAGCA TATGGAAACT 1051 GAATGATCTT CGTGACCTGA CACAATGTGT GTCCTTGTTC TTATTTGGAG 1101 AAGTTCACAA AGCGCTCTGG AAGACGGAGC AGGGGACTGT CGTAGGGATC 1151 CTCAATGCCA ACCCCATGAA GCCCAAGGAT GGTTCAGAGG AGGTGTGTTT 1201 ATCTATCGAT CATCCTCAGA AGGTCTTAAT TATGGGTGAA GCTCTTGACC 1251 TGGGAACCTG TAAAGCCAAG AAGAAGAATG GAGAGCCGTG CACGCAGACT 1301 GTGAATTTGC GTGACTGTGA GTACTGTCAG TACCATGTCC AGGCTCAGTA 1351 CAAGAAGCTC AGTGCAAAGC GTGCGGATCT GCAGTCCACC TTCTCTGGAG 1401 GACGAATTCC AAAGAAGTTT GCCCGCAGAG GCACCAGCCT CAAAGAACGG 1451 CTGTGCCAAG ATGGCTTTTA CTACGGAGGG GTTTCTTCTG CCTCGTATGC 1501 AGCTTCAATT GCAGCAGCTG TGGCTCCTAA GAAGAAGATT CAAACCACTC 1551 TGAGTAATCT GGTTGTTAAG GGCACAAACT TGATCATCCA GGAAACACGG 1601 CAAAAACTCG GAATACCCCA GAAGAGCCTG TCTTGCTCTG AGGAGTTCAA 1651 GGAACTGATG GACCTGCCGA CGTGTGGAGC CAGGAACTTA AAACAACATT 1701 TAGCCAAAGC CTCAGCTTCA GGGATTATGG GGAGCCCAAA ACCAGCCATC 1751 AAGTCCATCT CGGCCTCAGC ACTCTTGAAG CAACAGAAGC AGCGGATGTT 1801 GGAGATGAGG AGAAGGAAAT CAGAAGAAAT ACAGAAGCGA TTTCTGCAGA 1851 GCTCAAGTGA AGTTGAGAGC CCAGCTGTGC CATCTTCATC AAGACAGCCC 1901 CCTGCTCAGC CTCCACGGAC AGGATCCGAG TTCCCCAGGC TGGAGGGAGC 1951 CCCGGCCACA ATGACGCCCA AGCTGGGGCG AGGTGTCTTG GAAGGAGATG 2001 ATGTTCTCTT TTATGATGAG TCACCACCAC CAAGACCAAA ACTGAGTGCT 2051 TTAGCAGAAG CCAAAAAGTT AGCTGCTATC ACCAAATTAA GGGCAAAAGG 2101 CCAGGTTCTT ACAAAAACAA ACCCAAACAG CATTAAGAAG AAACAAAAGG 2151 ACCCTCAGGA CATCCTGGAG GTGAAGGAAC GTGTAGAAAA AAACACCATG 2201 TTTTCTTCTC AAGCTGAGGA TGAATTGGAG CCTGCCAGGA AAAAAAGGAG 2251 AGAACAACTT GCCTATCTGG AATCTGAGGA ATTTCAGAAA ATCCTAAAAG 2301 CAAAATCAAA ACACACAGGC ATCCTGAAAG AGGCCGAGGC TGAGATGCAG 2351 GAGCGCTACT TTGAGCCACT GGTGAAAAAA GAACAAATGG AAGAAAAGAT 2401 GAGAAACATC AGAGAAGTGA AGTGCCGTGT CGTGACATGC AAGACGTGCG 2451 CCTATACCCA CTTCAAGCTG CTGGAGACCT GCGTCAGTGA GCAGCATGAA 2501 TACCACTGGC ATGATGGTGT GAAGAGGTTT TTCAAATGTC CCTGTGGAAA 2551 CAGAAGCATC TCCTTGGACA GACTCCCGAA CAAGCACTGC AGTAACTGTG 2601 GCCTCTACAA ATGGGAACGG GACGGAATGC TAAAGGTATG CCATTTGCGT 2651 ACTAATTTTT GACTCCTTTT AGTGACCCAT GCTAATAATG TGGAACCATC 2701 TCCTATTAAA ATATTTTCAT TTTTCTAGGA AAAGACTGGT CCAAAGATAG 2751 GAGGAGAAAC TCTGTTACCA AGAGGAGAAG AACATGCTAA ATTTCTGAAC 2801 AGCCTTAAAT AACCCGAACT TCAGACATTT TCCCACAGAC TTCCTGGCCT 2851 CCTGTGACTC TGGAAAGCAA AGGATTGGCT GTGTATTGTC CATTGATTCC 2901 TGATTGACGC CGTCAAAAAC AAATGCTTGT TAAGCCCATA AGCTTTGCCT 2951 GCTTACTTTC TGCCATTGGG TTGGTTTGAT ACCACATTTA ACATTGACAT 3001 TTAAGTGGAA AACCAAGTTA TCATTGTCTT TTCTAAGCTC AGTGTGGATG 3051 ATTGCATTAC TTCATTCACT GAAGTTTTTG CCCAAAAATT GGAAGGTAAA 3101 CAGAGAGCTA TGTTTCTGTA TCTTTTGGTT ATAGAGTGTT CACTTCTTTA 3151 TCATAACAAA ATTCTAGTGT TTATACGAAC ACCCAGAGGC AAAAGAATTT 3201 GGCTTAATTC TCACTCCAGG TAAGTAGCTT AACTTCTGGG CTTCAGTTTT 3251 CTCATCTGTA AAATCAGGAA GATTGGACTA AGTGATCCTG AAATGTATTT 3301 TTTAGCACTG GATTTCTACA AATAATAAAA CTTTCCCATC TAGATAATGA 3351 TGATCACATA GTCTTGATGT ACGGACATTA AAAGCCAGAT TTCTTCATTC 3401 AATTCTGTTA TCTCTGTTTT ACTCTTTGAA ATTGATCAAG CCACTGAATC 3451 ACTTTGCATT TCAGTTTATA TATAGAGAGA GAAAGAAGGC TGTCTGCTCT 3501 TACATTATTG TGGAGCCCTG TGATAGAAAT ATGTAAAATC TCATATTATT 3551 TTTTTTTTAA TTTTTTTATT TTTTATGACA GGGTCTCACT ATGTCACCCT 3601 GGCTGGAGTG CAGTAGTGCG ATCGCGGCAC ACTGCAGCCT TGGCTTCCCT 3651 GGGCTCAAGC AGTCCTCCCA CCTCAGTCTC CCAAATAGCT AGGACTACAG 3701 GCGTGCGTGA CCAAGCCCAG CTAATTTTTG CATTTTTTGT AGAGATGGGG 3751 TTTTGCCATG TTGCTCAGGC TGGTCTCAAA CTCCTGAGCA CTAGCAATCC 3801 ACCCACCTCT GTTTCCAAAA AAAAAAAAAA AATGAAAGGT CAACCCCTAT 3851 GCAAATTACC ACAGCAAAGG TTTCATTCAG GAGATTCTTC CATCTGGGCA 3901 ACCTGGTTTT CCAAATATCA TTTGACCTAA GTGAATGTTG ATACTAGCTA 3951 AAGATTGGGT AAATTGGTTG AATTATTGTA TTGAAGCTTG AGCTGTAGCT 4001 AAAAGTAATT TAGGTTTCCC CTAAGATGTT ATTATGTTAG GGACATAACA 4051 CTTTTGGGAG GTTGTTGTGG GAGATGGTTG ATTTAGGTTT TCAAAAGCTA 4101 GAAATAAAAT TTACATGCCT TAGATTTCAT AAAATTCTGC TCTAATTGGG 4151 TGGAAGGTGC TGTATCTAAC TTGTGTTCCT CCTAAGGTTA TGTCCTAATA 4201 ACTATTCTTT TAGGAGTATA CTTCTACTTT ATAGAAGGTT GCTTTTCTTT 4251 TTAATTTTTT CTAACAAAGA AAAGAATAAA GTATTTATTA ATAAGAACCA 4301 GAAAGCACTT GAAACTGATG TTTTTAATGG CTCATTTAGG GTAGATTTAT 4351 TTATCTCATT AACTTAAAAC AGCTATGTGT ATGAAATAGG TCACAACAGA 4401 ACTTGAACAC CAGGTTGGTG TCTGAGCAAT CCCTTTCTTA TGGGAAAAAC 4451 AATGTTCTTG TTTGAACAGA GGGTATCATT GCAGTCAGTA TTCACGTGTA 4501 TATTGTTATA TAAGTTGTAT AATATGCTTG TAAAGGCTGA GGGTGAGCTG 4551 TATCTGGATG CCTTTTTACA ATTTGATTTT AACTTTTAAA ATAAATTTAA 4601 AACATAAAAA AAAAAAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 95 bp to 2659 bp; peptide length: 855 Category: similarity to known protein Classification: Cell division

1 MDEEEDNLSL LTALLEENES ALDCNSEENN FLTRENGEPD AFDELFDADG 51 DGESYTEEAD DGETGETRDE KENLATLFGD MEDLTDEEEV PASQSTENRV 101 LPAPAPRREK TNEELQEELR NLQEQMKALQ EQLKVTTIKQ TASPARLQKS 151 PEKSPRPPLK ERRVQRIQES TCFSAELDVP ALPRTKRVAR TPKPSPPDPK 201 SSSSRMTSAP SQPLQTISRN KPSGITRGQI VGTPGSSGET TQPICVEAFΞ 251 GLRLRRPRVS STEMNKKMTG RKLIRLSQIK EKMAREKLEE IDWVTFGVIL 301 KKVTPQSVNS GKTFSIWKLN DLRDLTQCVS LFLFGEVHKA LWKTEQGTVV 351 GILNANPMKP KDGSEEVCLS IDHPQKVLIM GEALDLGTCK AKKKNGEPCT 401 QTVNLRDCEY CQYHVQAQYK KLSAKRADLQ STFSGGRIPK KFARRGTSLK 451 ERLCQDGFYY GGVSSASYAA SIAAAVAPKK KIQTTLSNLV VKGTNLIIQE 501 TRQKLGIPQK SLSCSEEFKE LMDLPTCGAR NLKQHLAKAS ASGIMGSPKP 551 AIKSISASAL LKQQKQRMLE MRRRKSEEIQ KRFLQSSSEV ESPAVPSSSR 601 QPPAQPPRTG ΞEFPRLEGAP ATMTPKLGRG VLEGDDVLFY DESPPPRPKL 651 SALAEAKKLA AITKLRAKGQ VLTKTNPNSI KKKQKDPQDI LEVKERVEKN 701 TMFSΞQAEDE LEPARKKRRE QLAYLESEEF QKILKAKSKH TGILKEAEAE 751 MQERYFEPLV KKEQMEEKMR NIREVKCRVV TCKTCAYTHF KLLETCVSEQ 801 HEYHWHDGVK RFFKCPCGNR SISLDRLPNK HCSNCGLYKW ERDGMLKVCH 851 LRTNF BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_2hl5, frame 2

TREMBLNEW: SPBC1347_10 gene: "cdc23"; "SPBC1347.10"; product: "cell division cycle protein 23"; S. pombe chromosome II cosmid C1347., N = 2, Score = 284, P = 7e-21

PIR:S48384 DNA43 protein - yeast (Saccharomyces cerevisiae), N = 2, Score = 203, P = 7e-12

TREMBL :SCDNA52A_1 gene: "DNA52"; Saccharomyces cerevisiae DNA52 gene, complete eds., N = 2, Score = 201, P = 7.9e-12

TREMBLNEW:AC006234_6 gene: "F5H14.6"; Arabidopsis thaliana chromosome II BAC F5H14 genomic sequence, complete sequence., N = 2, Score = 211, P = 1.7e-15

PIR:S48384 DNA43 protein - yeast (Saccharomyces cerevisiae), N = 2, Score = 203, P = 7.2e-12

>TREMBLNEW:SPBC1347_10 gene: "cdc23"; "SPBC1347.10"; product: "cell division cycle protein 23"; S. pombe chromosome II cosmid cl347. Length = 593

HSPs:

Score = 284 (42.6 bits), Expect = 7.0e-21, Sum P(2) = 7.0e-21 Identities = 97/383 (25%), Positives = 186/383 (48%)

Query: 109 EKTNEELQEELRNLQEQMKALQEQLKVTTIKQTASPARLQKSPEKSPRPPLKERRVQRIQ 168

E+ + +L+E + LQ Q+ +QE+ ++ + ++ AS + + PR P ++ RV + Sbjct: 8 EENDLDLEE—KRLQRQLNEIQEKKRLRSAQKEASSENAEVI—QVPRSPPQQVRVLTVS 63

Query: 169 ESTCFSAE LDVPALPRTKRVARTPKPSPPDPKSSSSRMTSAPSQP LQTIS 218

+ + L + K V+ P P PK R+ A +Q L+T+ Sbjct: 64 SPSKLKSPKRLILGIDKGKTGKDVSLGKGPRGPLPKPFHERLAEARNQERKRSDKLKTMK 123

Query: 219 RNKPSGITRGQIVGTPGSSGETTQPI-C—VEAFSGLRLRRPRVSSTEMNKKMTGRKLIR 275

+N+ R + + G S E P+ C ++ +S + +Ξ + + G ++ Sbjct: 124 KNRKQSFQRKRNILEDGKΞEEEKFPMKCDEIDPYSRQAIVIRYISDEVAKENIGGNQVYL 183

Query: 276 LSQIKEKMAREKLE—EID-WVTFGVILKKV-TPQSVNSGKTFSIWKLNDLRDLTQCVSL 331

+ Q+ + + K E E+D +V G++ T ++VN K + + L DL+ +C Sbjct: 184 IHQLLKLVRAPKFEAPEVDNYVVMGIVASNSGTRETVNGNK-YCMLTLTDLKWQLEC 239

Query: 332 FLFGEVHKALWKTEQGTVVGILNANPMKPKDGS-EEVCLSIDHPQKVLI-MGEALDLGTC 389

FLFG+ + WK + GTV+ +LN +KPK+ L +D VL+ +G + LG C Sbjct: 240 FLFGKAFERYWKIQSGTVIALLNPEVLKPKNPDIGRFSLKLDSEYDVLLEIGRSKHLGYC 299

Query: 390 KAKKKNGEPCTQTVNLRDCEYCQYHVQAQYKKLSAKRADLQSTFSGGRIPKKFARRGTSL 449

+++K+GE C ++ R + C+YHV ++ + R + S+ + P+ ARR Sbjct: 300 SSRRKSGELCKHWLDKRAGDVCEYHVDLAVQRSMSTRTEFASSMATMHEPR—ARR 353

Query: 450 KERLCQDGF—YYGGVSSASYAASIAAAVAPKKKIQT 484

++R GF Y+ G ++ ++A + +QT Sbjct: 354 EKRFRGQGFQGYFAGEKYSAIPNAVAGLYDAEDAVQT 390

Score = 41 (6.2 bits), Expect = 7.0e-21, Sum P(2) = 7.0e-21 Identities = 12/43 (27%), Positives = 17/43 (39%)

Query: 453 LCQDGFYYGGVSSASYAASIAAAVAPKKKIQTTLSNLVVKGTN 495

L +D S AS A++ K + SN + GTN

Sb_jct: 465 LSKDSEIDSSTKKPSVLASFNASIMNPKSSLPSFSNSAILGTN 507

Score = 40 (6.0 bits), Expect = 8.9e-21, Sum P(2) = 8.9e-21 Identities = 13/26 (50%), Positives = 18/26 (69%)

Query: 536 LAKASASGIMGSPKPAIKSISASALL 561

LA +AS IM +PK ++ S S SA+L Sbjct: 481 LASFNAS-IM-NPKSSLPSFSNSAIL 504

Pedant information for DKFZphtes3_2hl5, frame 2

Report for DKFZphtes3_2hl5.2 [LENGTH] 855

[MW] 96135.01

[pi] 8.96

[HOMOL] TREMBLNEW: SPBC1347_10 gene: "cdc23"; "SPBC1347.10"; product: "cell division cycle protein 23"; S. pombe chromosome II cosmid C1347. 5e-16

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YIL150c] le-11

[FUNCAT] 03.16 dna synthesis and replication [S. cerevisiae, YIL150c] le-11

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YIL150c] le-11

[KW] Alpha_Beta

[KW] LOW_COMPLEXITY 12.05 %

[KW] COILED COIL 4.21 %

SEQ MDEEEDNLSLLTALLEENESALDCNSEENNFLTRENGEPDAFDELFDADGDGESYTEEAD SEG xxxxx PRD cccchhhhhhhhhhhhhhhhccccccccceeeeccccccccceeeecccccccceeeeec COILS

SEQ DGETGETRDEKENLATLFGDMEDLTDEEEVPASQSTENRVLPAPAPRREKTNEELQEELR SEG xxxxxxxxxxxx xxxxxxxxx PRD cccccccccccchhhhhhcccccccceeeccccccccccccccccccchhhhhhhhhhhh COILS CCCCCCCCCCCCCC

SEQ NLQEQMKALQEQLKVTTIKQTAΞPARLQKSPEKSPRPPLKERRVQRIQESTCFSAELDVP SEG xxxxx PRD hhhhhhhhhhhhhhhhhhhhhcccccccccccccccccccceeeeecccccccccccccc COILS CCCCCCCCCCCCCCCCCCCCCC

SEQ ALPRTKRVARTPKPSPPDPKSSSΞRMTSAPSQPLQTISRNKPSGITRGQIVGTPGSSGET SEG xxxxxxxxxxxxx PRD cccccceeeecccccccccccchhhhhhhccccchhhhhhccccccceeeeecccccccc COILS

SEQ TQPICVEAFSGLRLRRPRVSSTEMNKKMTGRKLIRLSQIKEKMAREKLEEIDWVTFGVIL SEG PRD cccccccccchhhhhhhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhccceeeeeeee COILS

SEQ KKVTPQSVNSGKTFSIWKLNDLRDLTQCVSLFLFGEVHKALWKTEQGTVVGILNANPMKP SEG PRD cccccccccccceeeeeeeccchhhhhhheeeeecchhhhhhhhccceeeeecccccccc COILS

SEQ KDGSEEVCLSIDHPQKVLIMGEALDLGTCKAKKKNGEPCTQTVNLRDCEYCQYHVQAQYK SEG PRD ccccceeeeecccccceeeccccccccccccccccccccceeecccccccchhhhhhhhh COILS

SEQ KLSAKRADLQSTFSGGRIPKKFARRGTSLKERLCQDGFYYGGVSSASYAASIAAAVAPKK SEG xxxxxxxxxxxxxxxxxxx ... PRD hhhhhhhhhhhhccccccccccccccchhhhhhhccccccccccchhhhhhhhhhhhcch COILS

SEQ KIQTTLSNLVVKGTNLIIQETRQKLGIPQKSLSCSEEFKELMDLPTCGARNLKQHLAKAS SEG PRD hhhhhhheeecccceeeehhhhhhhcccccccchhhhhhhhhhccccccchhhhhhhhhh COILS

SEQ AΞGIMGSPKPAIKSISAΞALLKQQKQRMLEMRRRKSEEIQKRFLQSSSEVESPAVPSSSR SEG xxxxxxxxxxxxxxx PRD hhcccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccccc COILS

SEQ QPPAQPPRTGSEFPRLEGAPATMTPKLGRGVLEGDDVLFYDESPPPRPKLSALAEAKKLA SEG xxxxxxxx xxxxxxxxxxxx PRD ccccccccccccccccccccccccccccccccccceeeeeccccccchhhhhhhhhhhhh COILS

SEQ AITKLRAKGQVLTKTNPNSIKKKQKDPQDILEVKERVEKNTMFSΞQAEDELEPARKKRRE SEG xxxxx PRD hhhhhhhhheeeeecccccccccccccchhhhhhhhhhhhccchhhhhhhhhhhhhhhhh COILS

SEQ QLAYLESEEFQKILKAKSKHTGILKEAEAEMQERYFEPLVKKEQMEEKMRNIREVKCRVV SEG PRD hhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhheee COILS

SEQ TCKTCAYTHFKLLETCVSEQHEYHWHDGVKRFFKCPCGNRSISLDRLPNKHCSNCGLYKW SEG

PRD eeecceeeeeeecccceeeccccccccceeeeeecccccccccccccccccccccceeec

COILS

SEQ ERDGMLKVCHLRTNF

SEG

PRD ccccccccccccccc

COILS

(No Prosite data available for DKFZphtes3_2hl5.2) (No Pfam data available for DKFZphtes3_2hl5.2)

DKFZphtes3_2ι5

group: testes derived

DKFZphtes3_2ι5 encodes a novel 151 amino acid protein with weak similarity to. C. elegans cosmid F20D12.3

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . similarity to C. elegans F20D12.3 many ATGs in front of the start of the ORF, unspliced intron in 5' region?

Sequenced by EMBL

Locus : unknown

Insert length: 2142 bp

Poly A stretch at pos. 2121, polyadenylation signal at pos. 2102

1 GCAGTAAATA TGATATGAAA GAATTCTCTA ACTTGGGGGT GGCTTGTAAC

51 CTGTAATAAA AATATTGCTA AAATACCTTC TCTCACTTTG AAAAAGCATC

101 TGAGCAATCC TCAGTTATTG GTGAATTCTT ACCAGTGTTT AATTCCTCTC

151 TTTCCGTTAT GGTCTTAGTG TGGTTGTCCT GGTGTAGTAT TTCAAGAGGA

201 ACCTGCAGCA AGATGAAAAG AGAGTGGGAC TTGGAGCTAA GAACGTTTTT

251 GGCTTTAAGT GCTACGTTAA CTCATTAAAT TCTTAGTGAT CTTGGGGAAG

301 TCCCCTCACC AGTGTGAGCC TCAGTTTTCT TATCTAATAA GTAAGGATAA

351 TCTTACCCAC CTTATTGCGG GGGCCCGAGG ATTACATGAT TGGTGTAACA

401 GTAGCACCTT GTACATTTGA AAGGACTAAT ACCAGTGGAC TTTAACCTTG

451 GCTGGGCTTT GGAATTCTTG GTGGGACTTT TTAATCATGT AGATTCTCAG

501 GCCCCTGCCT GGCCTGTGGA ACCACAGACT CTATAGGTGG GCCCTTCCAG

551 AAGGCCTCAT GGGTGGTTCT CATGTGGAAC CTGTGTTGCA AGCCACTGCA

601 TGGTGTTACT GCTATTAACA TTAAAACTTA TATTTTCCTT ATTGTGTGGA

651 TATATCTGTG GTGTTTGCCC ATGTATACTT CATTTTACAT TTCTTAAAGA

701 ATAGAATGGA ATGGTTTTAA GCACGCTACA TTGTCCAGGT TATACCCACA

751 GAAGAGCTGT TGTGTAACAG AATCAGCATC ATACCTGAAT CATTTGTACA

801 TTGCATATAA GACTATGTCT AAGTAGAAGA TGCTATGAAA TCATGTCTGC

851 TGTGGGGCCA GGCATAATTA TGAATGTTAC TTAAGAGCAT AGGTGAGGTG

901 AGAAAAGGGA ATGTGACTAG TGTTTTAGTA TTTTCTTGGT GTGGGATGAA

951 GTATAATTCT TTTTTTTTTT TCTCAACAAA GCAGTAAAAC TAGAAAGAAG

1001 GAGAACTCTT CCCTCAAGAA TGGCTGTACC TTCATATCTA GAGGCACATT

1051 AAAAAAAAGA ACGTCTGTAC CTTAAAAATG GAGGTCATTT CATTGTGTTC

1101 ATTTTCAAGG TTGTTGTATG GCTCGGTCAG AACTTTCTGT TACCAGAAGA

1151 CACTCACATT CAGAATGCTC CATTTCAAGT GTGTTTCACA TCTTTACGGA

1201 ATGGCGGCCA CCTGCATATA AAAATAAAAC TTAGTGGAGA GATCACTATA

1251 AATACTGATG ATATTGATTT GGCTGGTGAT ATCATCCAGT CAATGGCATC

1301 ATTTTTTGCT ATTGAAGACC TTCAAGTAGA AGCGGATTTT CCTGTCTATT

1351 TTGAGGAATT ACGAAAGGTG CTAGTTAAGG TGGATGAATA TCATTCAGTG

1401 CATCAGAAGC TCAGTGCTGA TATGGCTGAT CATTCTAATT TGATCCGAAG

1451 TTTGCTGGTC GGAGCTGAGG ATGCTCGTCT GATGAGGGAC ATGAAAACAA

1501 TGAAGAGTCG TTATATGGAA CTCTATGACC TTAATAGAGA CTTGCTAAAT

1551 GGATATAAAA TTCGCTGTAA CAATCACACA GAGCTGTTGG GAAACCTCAA

1601 AGCAGTAAAT CAAGCAATTC AAAGAGCAGG TCGTCTGCGG GTTGGAAAAC

1651 CAAAGAACCA GGTGATCACT GCTTGTCGGG ATGCAATTCG AAGCAATAAC

1701 ATCAACACAC TGTTCAAAAT CATGCGAGTG GGGACAGCTT CTTCCTAGGT

1751 GAGGAAAATA CAGGTCATGA AGTTCCTGGC AAAGATTTTC TGTTAAAAAC

1801 CTATGCTGGT TTGCTTTGGA TCACACCCTG GTGAACCCCG GGTGCTAAGA

1851 ATGAAAATAA CCTTGGTGAG TTGTACAAAT TAAAGACAAA GAACTACATG

1901 TGAAGATAGA CTTGCTTTCT ATTTTTAAAT CAGTAGTAGT ACTGTTGCTG

1951 AATAATACTA GGTTTTTATG GAATAGGATG AATGCTTTTG AAGTATTAGG

2001 GCTTCAGAGT CCAATTTTGC TTATTTATGG TATATAAATA CATATTTTTT

2051 TCTTGAAATT GCAATTGAGT TTGTACTTTT CAAATAGATT ATCTACTTTT

2101 TCATTAAAAT GTAAAGATGT TAAAAAAAAA AAAAAAAAAA AA

BLAST Results

No BLAST result

Medline entries No Medline entry

Peptide information for frame 3

ORF from 1293 bp to 1745 bp; peptide length: 151 Category: similarity to unknown protein Classification: no clue

1 MASFFAIEDL QVEADFPVYF EELRKVLVKV DEYHSVHQKL SADMADHSNL

51 IRSLLVGAED ARLMRDMKTM KSRYMELYDL NRDLLNGYKI RCNNHTELLG

101 NLKAVNQAIQ RAGRLRVGKP KNQVITACRD AIRSNNINTL FKIMRVGTAS 151 S

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_2ι5, frame 3

TREMBL :CEF20D12_1 gene: "F20D12.3"; Caenorhabditis elegans cosmid F20D12., N = 1, Score = 173, P = 4.5e-12

>TREMBL:CEF20D12_1 gene: "F20D12.3"; Caenorhabditis elegans cosmid F20D12. Length = 699

HSPs:

Score = 173 (26.0 bits), Expect = 4.5e-12, P = 4.5e-12 Identities = 33/130 (25%), Positives = 72/130 (55%)

Query: 20 FEELRKVLVKVDEYHSVHQKLSADMADHSNLIRSLLVGAEDARLMRDMKTMKSRYMELYD 79 F+E ++L ++D V +L+A++ + ++ +++ AED+ + ++ + Y+ L Sbjct: 569 FKEADEILEEIDPMTEVRDRLTAELQERQAAVKEIIIRAEDSIAIDNIPDARKFYIRLKA 628 Query: 80 LNRDLLNGYKIRCNNHTELLGNLKAVNQAIQRAGRLRVGKPKNQVITACRDAIRSNNINT 139 + ++R NN + +L+ +N+ 1+ RLRVG+P Q++ +CR Al +N Sbjct: 629 NDAAARQAAQLRWNNQERCVKΞLRRLNKIIENCSRLRVGEPGRQIVVSCRSAIADDNKQI 688 Query: 140 LFKIMRVGTA 149

+ KI++ G + Sbjct: 689 ITKILQYGAS 698

Pedant information for DKFZphtes3_2ι5, frame 3

Report for DKFZphtes3_2ι5.3

[LENGTH] 151 [MW] 17304.07 [pi] 9.33 [HOMOL] TREMBL :CEF20D12_1 gene: "F20D12.3"; Caenorhabditis elegans cosmid F20D12. 2e-12

[KW] Alpha_Beta

SEQ MASFFAIEDLQVEADFPVYFEELRKVLVKVDEYHSVHQKLSADMADHSNLIRSLLVGAED PRD ccceeeehhhhhhccccchhhhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ ARLMRDMKTMKΞRYMELYDLNRDLLNGYKIRCNNHTELLGNLKAVNQAIQRAGRLRVGKP PRD hhhhhccchhhhhheeeccchhhhhhheeeeeccchhhhhhhhhhhhhhhhhcccccccc

SEQ KNQVITACRDAIRSNNINTLFKIMRVGTASS PRD cceeeeehhhhhhcccceeeeccceeecccc

(No Prosite data available for DKFZphtes3_2ι5.3) (No Pfam data available for DKFZphtes3_2ι5.3) DKFZphtes3_2119

group: testes derived

DKFZphtes3_2119 encodes a novel 166 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, no EST hits

Sequenced by EMBL

Locus : unknown

Insert length: 1079 bp

Poly A stretch at pos. 1053, polyadenylation signal at pos. 1038

1 CCACAGGACA CACTGTTCCC AGGGCACAGA CACCCTGGGC TTTGGTTGGG 51 TCTTGGCCTC CAGGTAGGGC CCTGTTGGGC AGCGGGCAGC AACTCCTGAG

101 ACACTACTGT GATTCTTGGT GGTGGCTGTG GTAAAAAACC TGCAGGGCTA

151 GAGTTTGGGG TGAGATTCAG CAGTAACTGT GGCCTCTCCT AGTGACAGTA

201 TGTCACTCCC ACTCCCAGCA CGCATGCCCA CAGGCCACGG CCTCCACATC

251 ACAAACCCCC CACCAAGTTG CCCATCTATG GAGCAGCTCC CATACGGCAG

301 GGTCAGGCTC TTACCTCCAC CTCCAGGGCA CAGACAGGGG GAGCTCTGTC

351 TCACTGTAAG GCAATGAGGA GAGTTGAGGG CCCAGACCAG GCTAGGGGCC

401 ATCCCCTTTC CCGAGCAGGC CTCAGGGAAG GACCAGCCCC ATTCCCATCT

451 GACCTAGGTC TTAGCCCAGG AGCCTGCATA GGGAAGAAAG GACAGACAGG

501 GCCTCCTTAC TGGCTGACAC TCAGGAGGGG CTGGGGCAAG AGAGCAGAGG

551 GAGCGCAGGG CCAGGCAGGG GCTGCTGAGG ATCCATGGGA GCTCAGGGTG

601 CACAAGGGGG CTGCCCTTCC TGGGCTGCAG GCAGCATCCC TATGGGAGCT

651 GAGAAAGTCC AATCCTGAGA TGGGACAGTG CTGCCCAGGG GTGTGTGGCT

701 GGGCCCTGAC AACAGTCTCC CCAAAAGTGA CCACATCACC AGGCTCAGTT

751 CCAGGAAGGC TGAGAAGTGC CCAGTACACT GAGGATGCAC CTCAGTTACA

801 TAAAATAAAT GAAACTGGAG TACTAACGTA CAGTTTAAAG GTTATAGTTA

851 CTATTTTTAT ATGATATACT AGTAATTTTT GAATAGGGTA AACTTTAGGT

901 GTTTTGACAC CAAAAGAAAA CTACATGAGT TCATGCATGT GTTAAATTGC

951 TTTACTGTAG TAATCATTTA CATGTATATG TATATATGAA TATAATTATG 1001 GGCTCATTAA ATTTAAATAT TATAAATAGG TGACAAAGAA TAAAGTTAAC 1051 TGGAAAAAAA AAAAAAAAAA AAAAAAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 1

ORF from 364 bp to 861 bp; peptide length: 166 Category: putative protein Classification: no clue

1 MRRVEGPDQA RGHPLSRAGL REGPAPFPSD LGLSPGACIG KKGQTGPPYW

51 LTLRRGWGKR AEGAQGQAGA AEDPWELRVH KGAALPGLQA ASLWELRKSN

101 PEMGQCCPGV CGWALTTVSP KVTTSPGSVP GRLRSAQYTE DAPQLHKINE 151 TGVLTYSLKV IVTIFI

BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_2119, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphtes3_2119, frame 1

Report for DKFZphtes3_2119.1

[LENGTH] 166

[MW] 17691.35

[pi] 9.54

[KW] All Beta

[KW] LOW COMPLEXITY 7.23 %

SEQ MRRVEGPDQARGHPLSRAGLREGPAPFPSDLGLSPGACIGKKGQTGPPYWLTLRRGWGKR

SEG

PRD ccccccccccccccccccccccccccccccccccccceeeccccccccceeeeecccccc

SEQ AEGAQGQAGAAEDPWELRVHKGAALPGLQAASLWELRKSNPEMGQCCPGVCGWALTTVSP

SEG xxxxxxxxxxxx

PRD ccccccccccccccceeeeccccccccchhhhhhhhhhcccccccccccccceeeeeccc

SEQ KVTTSPGSVPGRLRSAQYTEDAPQLHKINETGVLTYSLKVIVTIFI

SEG

PRD ccccccccccccccccccccccccceeeccccceeeehhhhhhccc

(No Prosite data available for DKFZphtes3_2119.1) (No Pfam data available for DKFZphtes3_2119.1)

DKFZphtes3_2ml8

group: nucleic acid management

DKFZphtes3_2ml8 encodes a novel amino acid protein, with similarity to mouse Dhml .

The protein seems to play a role in nucleotide metabolism, RNA metabolism, but also in DNA repair and cell cycle. The yeast homologue is a DNA strand exchange protein required for sporulation and homologous recombination.

The novel protein can find application as multifunctional nuclease / exoribonuclease. nearly identical to mouse Dhml complete cDNA, complete eds, start at Bp 42, EST hits

Sequenced by EMBL

Locus: unknown

Insert length: 3022 bp

Poly A stretch at pos. 3004, polyadenylation signal at pos. 2981

1 CTCGTCAGCC GGTCGGCCGC CGCCTCCAGC CGTGTGCCGC TATGGGAGTC

51 CCGGCGTTCT TCCGCTGGCT CAGCCGCAAG TACCCGTCCA TCATAGTCAA

101 CTGCGTGGAA GAGAAGCCAA AAGAATGCAA TGGTGTAAAG ATTCCAGTTG

151 ATGCCAGTAA ACCTAATCCA AATGATGTGG AGTTTGATAA TCTGTATTTG

201 GATATGAATG GAATCATCCA TCCCTGTACT CATCCTGAAG ACAAACCAGC

251 ACCAAAAAAT GAAGATGAAA TGATGGTTGC AATTTTTGAG TACATTGACA

301 GACTTTTCAG TATTGTAAGA CCAAGAAGAC TTCTCTACAT GGCAATAGAT

351 GGAGTGGCAC CACGTGCTAA AATGAACCAG CAGCGTTCAA GGAGGTTCAG

401 GGCATCAAAA GAAGGAATGG AAGCAGCAGT CGAGAAGCAG CGAGTCAGGG

451 AAGAAATATT GGCAAAAGGT GGCTTTCTTC CTCCAGAAGA AATAAAAGAA

501 AGATTTGACA GCAACTGTAT TACACCAGGA ACTGAATTCA TGGACAATCT

551 TGCTAAATGC CTTCGCTATT ACATAGCTGA TCGTTTAAAT AATGACCCTG

601 GGTGGAAAAA TTTGACAGTT ATTTTATCTG ATGCTAGTGC TCCTGGTGAA

651 GGAGAACATA AAATCATGGA TTACATTAGA AGGCAAAGAG CCCAGCCTAA

701 CCATGACCCA AATACTCATC ATTGTTTATG TGGAGCAGAT GCTGATCTCA

751 TTATGCTTGG CCTTGCCACA CATGAACCGA ACTTTACCAT TATTAGAGAA

801 GAATTCAAAC CAAACAAGCC CAAACCATGT GGTCTTTGTA ATCAGTTTGG

851 ACATGAGGTC AAAGATTGTG AAGGTTTGCC AAGAGAAAAG AAGGGAAAGC

901 ATGATGAACT TGCCGATAGT CTTCCTTGTG CAGAAGGAGA GTTTATCTTC

951 CTTCGGCTTA ATGTTCTTCG TGAGTATTTG GAAAGAGAAC TCACAATGGC

1001 CAGCCTACCA TTCACATTTG ATGTTGAGAG GAGCATTGAT GACTGGGTTT

1051 TCATGTGCTT CTTTGTGGGA AATGACTTCC TCCCTCATTT GCCATCGTTA

1101 GAGATTAGGG AAAATGCAAT TGACCGTTTG GTTAACATAT ACAAAAATGT

1151 GGTACACAAA ACTGGGGGTT ACCTTACAGA AAGTGGTTAT GTCAATCTGC

1201 AAAGAGTACA GATGATCATG TTAGCAGTTG GTGAAGTTGA GGATAGCATT

1251 TTTAAAAAGA GAAAGGATGA TGAGGACAGT TTTAGAAGAC GACAGAAAGA

1301 AAAAAGAAAG AGAATGAAGA GAGATCAACC AGCTTTCACT CCTAGTGGAA

1351 TATTAACTCC TCATGCCTTG GGTTCAAGAA ATTCACCAGG TTCTCAAGTA

1401 GCCAGTAATC CGAGACAAGC AGCCTATGAA ATGAGGATGC AGAATAACTC

1451 TAGTCCTTCG ATATCTCCTA ATACGAGTTT CACATCTGAT GGCTCCCCGT

1501 CTCCATTAGG AGGAATTAAG CGAAAAGCAG AAGACAGTGA CAGTGAACCT

1551 GAGCCAGAGG ATAATGTCAG GTTATGGGAA GCTGGCTGGA AGCAGCGGTA

1601 CTACAAGAAC AAATTTGATG TGGATGCAGC TGATGAGAAA TTCCGTCGGA

1651 AAGTTGTGCA GTCGTACGTT GAAGGACTTT GCTGGGTTCT TAGATATTAT

1701 TACCAGGGCT GTGCTTCCTG GAAGTGGTAT TATCCATTTC ATTATGCACC

1751 ATTTGCTTCA GACTTTGAAG GCATTGCAGA CATGCCATCT GATTTTGAGA

1801 AGGGTACGAA ACCGTTTAAA CCACTAGAAC AACTTATGGG GGTATTTCCA

1851 GCTGCAAGTG GTAATTTTCT ACCTCCATCA TGGCGGAAGC TCATGAGTGA

1901 TCCTGATTCT AGTATAATTG ACTTCTATCC TGAAGATTTT GCTATTGATT

1951 TGAATGGGAA GAAATATGCA TGGCAAGGTG TTGCTCTCTT GCCATTCGTG

2001 GATGAGCGAA GGCTACGAGC TGCCCTAGAA GAGGTATACC CAGACCTCAC

2051 TCCAGAAGAG ACCAGAAGAA ACAGCCTTGG AGGTGATGTC TTATTTGTGG

2101 GGAAACATCA CCCACTCCAT GACTTCATTT TAGAGCTGTA CCAGACAGGT

2151 TCCACAGAGC CAGTGGAGGT ACCCCCTGAA CTATGTCATG GGATTCAAGG

2201 AAAGTTTTCT TTGGATGAAG AAGCCATTCT TCCAGATCAA ATAGTATGTT

2251 CTCCTGTTCC TATGTTAAGG GATCTGACAC AGAACACTGT AGTCAGTATT

2301 AATTTTAAAG ACCCACAGTT TGCTGAAGAT TACATTTTTA AAGCTGTAAT

2351 GCTTCCAGGA GCAAGAAAGC CAGCAGCAGT ACTGAAACCT AGTGACTGGG

2401 AAAAATCCAG CAATGGACGG CAGTGGAAGC CTCAGCTTGG CTTTAACCGT

2451 GACCGGAGGC CTGTGCACCT GGATCAGGCA GCCTTCAGGA CTTTGGGCCA

2501 TGTGATGCCA AGAGGCTCAG GAACTGGCAT TTACAGCAAT GCTGCACCAC

2551 CACCTGTGAC TTACCAGGGA AACTTATACA GGCCGCTTTT GAGAGGACAA

2601 GCCCAGATTC CAAAACTTAT GTCAAATATG AGGCCCCAGG ATTCCTGGCG

2651 AGGTCCTCCT CCCCTTTTCC AGCAGCAAAG GTTTGACAGA GGCGTTGGGG 2701 CTGAACCTCT GCTCCCATGG AACCGGATGC TGCAAACCCA GAATGCAGCC

2751 TTCCAGCCAA ACCAGTACCA GATGCTAGCT GGGCCTGGTG GGTATCCACC

2801 CAGACGAGAT GATCGTGGAG GGAGACAGGG ATATCCCAGA GAAGGAAGGA

2851 AATACCCTTT GCCACCACCC TCAGGAAGAT ACAATTGGAA TTAAGCTTTT

2901 GTAAAGCTTT CCCAAATCCT TTCATCATTC TACAGTTTTA TGCTATTTGT

2951 GGAAAGATTT CCTTCTCAAG TAGTAGTTTT TAATAAAACT ACAGTACTTT

3001 GTGTAAAAAA AAAAAAAAAA AA

BLAST Results

No BLAST result

Medline entries

95192042:

Characterization of cDNA encoding mouse homolog of fission yeast dhpl÷ gene: structural and functional conservation.

97361754:

Cloning and characterization of mouse Dhm2 cDNA, a functional homolog of budding yeast

SEP1.

Peptide information for frame 3

ORF from 42 bp to 2891 bp; peptide length: 950 Category: strong similarity to known protein

1 MGVPAFFRWL SRKYPSIIVN CVEEKPKECN GVKIPVDASK PNPNDVEFDN 51 LYLDMNGIIH PCTHPEDKPA PKNEDEMMVA IFEYIDRLFS IVRPRRLLYM 101 AIDGVAPRAK MNQQRSRRFR ASKEGMEAAV EKQRVREEIL AKGGFLPPEE 151 IKERFDSNCI TPGTEFMDNL AKCLRYYIAD RLNNDPGWKN LTVILSDASA 201 PGEGEHKIMD YIRRQRAQPN HDPNTHHCLC GADADLIMLG LATHEPNFTI 251 IREEFKPNKP KPCGLCNQFG HEVKDCEGLP REKKGKHDEL ADSLPCAEGE 301 FIFLRLNVLR EYLERELTMA SLPFTFDVER ΞIDDWVFMCF FVGNDFLPHL 351 PSLEIRENAI DRLVNIYKNV VHKTGGYLTE SGYVNLQRVQ MIMLAVGEVE 401 DSIFKKRKDD EDSFRRRQKE KRKRMKRDQP AFTPSGILTP HALGSRNSPG 451 SQVASNPRQA AYEMRMQNNS SPSISPNTSF TSDGSPSPLG GIKRKAEDSD 501 SEPEPEDNVR LWEAGWKQRY YKNKFDVDAA DEKFRRKVVQ SYVEGLCWVL 551 RYYYQGCASW KWYYPFHYAP FASDFEGIAD MPSDFEKGTK PFKPLEQLMG 601 VFPAASGNFL PPΞWRKLMSD PDSΞIIDFYP EDFAIDLNGK KYAWQGVALL 651 PFVDERRLRA ALEEVYPDLT PEETRRNΞLG GDVLFVGKHH PLHDFILELY 701 QTGSTEPVEV PPELCHGIQG KFSLDEEAIL PDQIVCSPVP MLRDLTQNTV 751 VSINFKDPQF AEDYIFKAVM LPGARKPAAV LKPSDWEKΞS NGRQWKPQLG 801 FNRDRRPVHL DQAAFRTLGH VMPRGSGTGI YSNAAPPPVT YQGNLYRPLL 851 RGQAQIPKLM SNMRPQDSWR GPPPLFQQQR FDRGVGAEPL LPWNRMLQTQ 901 NAAFQPNQYQ MLAGPGGYPP RRDDRGGRQG YPREGRKYPL PPPSGRYNWN

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_2ml8, frame 3

PIR: 149635 mouse Dhml protein - mouse, N = 1, Score = 4765, P = 0

PIR:S43891 dhpl protein - fission yeast (Schizosaccharomyces pombe), N = 3, Score = 1172, P = 2e-197

PIR:S20126 exoribonuclease RAT1 (EC 3.1.11.-) - yeast (Saccharomyces cerevisiae), N = 2, Score = 1146, P = 3.8e-175

PIR:S72531 exonuclease II - fission yeast (Schizosaccharomyces pombe), N = 4, Score = 622, P = 4.2e-125

>PIR: 149635 mouse Dhml protein Length = 947

HSPs: Score = 4765 (714.9 bits), Expect = O.Oe+00, P = O.Oe+00 Identities = 884/930 (95%), Positives = 895/930 (96%)

Query: 1 MGVPAFFRWLSRKYPSIIVNCVEEKPKECNGVKIPVDASKPNPNDVEFDNLYLDMNGIIH 60

MGVPAFFRWLSRKYPSIIVNCVEEKPKECNGVKIPVDASKPNPNDVEFDNLYLDMNGIIH Sb_jct: 1 MGVPAFFRWLSRKYPSIIVNCVEEKPKECNGVKIPVDASKPNPNDVEFDNLYLDMNGIIH 60

Query: 61 PCTHPEDKPAPKNEDEMMVAIFEYIDRLFSIVRPRRLLYMAIDGVAPRAKMNQQRSRRFR 120

PCTHPEDKPAPKNEDEMMVAIFEYIDRLF+IVRPRRLLYMAIDGVAPRAKMNQQRSRRFR Sbjct: 61 PCTHPEDKPAPKNEDEMMVAIFEYIDRLFNIVRPRRLLYMAIDGVAPRAKMNQQRSRRFR 120

Query: 121 ASKEGMEAAVEKQRVREEILAKGGFLPPEEIKERFDSNCITPGTEFMDNLAKCLRYYIAD 180

A K GMEAAVEKQRVREEILAKGGFLPPEEIKERFDSNCITPGTEFMDNLAKCLRYYIAD Sbjct: 121 AIKGGMEAAVEKQRVREEILAKGGFLPPEEIKERFDSNCITPGTEFMDNLAKCLRYYIAD 180

Query: 181 RLNNDPGWKNLTVILSDASAPGEGEHKIMDYIRRQRAQPNHDPNTHHCLCGADADLIMLG 240

RLNNDPGWKNLTVILSDASAPGEGEHKIMDYIRRQRAQPN DPNTHHCLCGADADLIMLG Sbjct: 181 RLNNDPGWKNLTVILSDASAPGEGEHKIMDYIRRQRAQPNQDPNTHHCLCGADADLIMLG 240

Query: 241 LATHEPNFTIIREEFKPNKPKPCGLCNQFGHEVKDCEGLPREKKGKHDELADSLPCAEGE 300

LATHEPNFTIIREEFKPNKPKPC LCNQFGHEVKDCEGLPREKKGKHDELADSLPCAEGE Sbjct: 241 LATHEPNFTIIREEFKPNKPKPCALCNQFGHEVKDCEGLPREKKGKHDELADΞLPCAEGE 300

Query: 301 FIFLRLNVLREYLERELTMASLPFTFDVERSIDDWVFMCFFVGNDFLPHLPSLEIRENAI 360

FIFLRLNVLREYLERELTMASLPF FDVERS DDW FMCFFVGNDFLPHLPSLEIRE Al Sbjct: 301 FIFLRLNVLREYLERELTMASLPFPFDVERSNDDWEFMCFFVGNDFLPHLPSLEIREGAI 360

Query: 361 DRLVNIYKNVVHKTGGYLTESGYVNLQRVQMIMLAVGEVEDSIFKKRKDDEDSFRRRQKE 420

DRLVNIYKNVVHKTGGYLTESGYVNLQRVQMIMLAVGEVEDSIFKKRKDDEDSFRRRQKE Sbjct: 361 DRLVNIYKNVVHKTGGYLTESGYVNLQRVQMIMLAVGEVEDΞIFKKRKDDEDSFRRRQKE 420

Query: 421 KRKRMKRDQPAFTPSGILTPHALGSRNSPGSQVAΞNPRQAAYEMRMQNNSSPSISPNTSF 480

KRKRMKRDQPAFTPSGILTPHALGSRNSPG QVASNPRQAAYEMRMQ NSSPSISPNTSF Sbjct: 421 KRKRMKRDQPAFTPSGILTPHALGSRNSPGCQVASNPRQAAYEMRMQRNSSPSISPNTSF 480

Query: 481 TSDGSPSPLGGIKRKAEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDAADEKFRRKVVQ 540

SDGSPSPLGGI+RKAEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDAADEKFRRKVVQ Sbjct: 481 ASDGSPSPLGGIRRKAEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDAADEKFRRKVVQ 540

Query: 541 SYVEGLCWVLRYYYQGCASWKWYYPFHYAPFASDFEGIADMPSDFEKGTKPFKPLEQLMG 600

SYVEGLCWVLRYYYQGCASWKW YPFHYAPFASDFEGIADM S+FEKGTKPFKPLEQLMG Sbjct: 541 SYVEGLCWVLRYYYQGCASWKWLYPFHYAPFASDFEGIADMSSEFEKGTKPFKPLEQLMG 600

Query: 601 VFPAASGNFLPPSWRKLMSDPDSSIIDFYPEDFAIDLNGKKYAWQGVALLPFVDERRLRA 660

VFPAASGNFLPP+WRKLMSDPDSSIIDFYPEDFAIDLNGKKYAWQGVALLPFVDERRLRA Sbjct: 601 VFPAASGNFLPPTWRKLMSDPDSSIIDFYPEDFAIDLNGKKYAWQGVALLPFVDERRLRA 660

Query: 661 ALEEVYPDLTPEETRRNSLGGDVLFVGKHHPLHDFILELYQTGSTEPVEVPPELCHGIQG 720

ALEEVYPDLTPEE RRNSLGGDVLFVGK HPL DFILELYQTGSTEPV+VPPELCHGIQG Sbjct: 661 ALEEVYPDLTPEENRRNSLGGDVLFVGKLHPLRDFILELYQTGSTEPVDVPPELCHGIQG 720

Query: 721 KFSLDEEAILPDQIVCSPVPMLRDLTQNTVVSINFKDPQFAEDYIFKAVMLPGARKPAAV 780

FSLDEEAILPDQ VCSPVPMLRDLTQNT VSINFKDPQFAEDY+FKA MLPGARKPA V Sbjct: 721 TFSLDEEAILPDQTVCSPVPMLRDLTQNTAVSINFKDPQFAEDYVFKAAMLPGARKPATV 780

Query: 781 LKPSDWEKSSNGRQWKPQLGFNRDRRPVHLDQAAFRTLGHVMPRGSGTGIYSNAAPPPVT 840

LKP DWEKSSNGRQWKPQLGFNRDRRPVHLDQAAFRTLGHV PRGSGT +Y+N A P Sbjct: 781 LKPGDWEKSSNGRQWKPQLGFNRDRRPVHLDQAAFRTLGHVTPRGSGTSVYTNTALLPAN 840

Query: 841 YQGNLYRPLLRGQAQIPKLMSNMRPQDSWRGPPPLFQQQRFDRGVGAEPLLPWNRMLQTQ 900

YQGN YRPLLRGQAQIPKLMSNMRP+DSWRGPPPLFQQ RF+R VGAEPLLPWNRM+Q Q Sbjct: 841 YQGNNYRPLLRGQAQIPKLMSNMRPKDΞWRGPPPLFQQHRFERSVGAEPLLPWNRMIQNQ 900

Query: 901 NAAFQPNQYQMLAGPGGYPPRRDD-RGGRQ 929

NAAFQPNQYQML GPGGYPPRRDD RGGRQ Sbjct: 901 NAAFQPNQYQMLGGPGGYPPRRDDHRGGRQ 930

Pedant information for DKFZphtes3_2ml8, frame 3

Report for DKFZphtes3_2ml8.3

[LENGTH] 950

[MW] 108582.68

[pl] 7.26

[HOMOL] PIR: 149635 mouse Dhml protein - mouse 0.0

[ FUNCAT] 08.01 nuclear transport [S. cerevisiae, YOR048C] le-123

[FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YOR048c] le-123 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YOR048c] le-123

[FUNCAT] 01.03.16 polynucleotide degradation [S. cerevisiae, YGL173c] 3e- 79

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YGL173c] 3e- 79

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YGL173c] 3e- 79

[PIRKW] nucleus le-126

[PIRKW] hydrolase le-122

[PIRKW] exoribonuclease le-122

[PROSITE] MYRISTYL 7

[PROSITE] AMIDATION 2

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 12

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE 8

[PROSITE] ASN_GLYCOSYLATION 4

[KW] TRANSMEMBRANE 1

[KW] LOW COMPLEXITY 6.21 %

SEQ MGVPAFFRWLSRKYPSIIVNCVEEKPKECNGVKIPVDASKPNPNDVEFDNLYLDMNGIIH SEG PRD cccchhhhhhhhhcceeeeeeecccccccccccccccccccccccccccceeeeccceee MEM

SEQ PCTHPEDKPAPKNEDEMMVAIFEYIDRLFSIVRPRRLLYMAIDGVAPRAKMNQQRSRRFR SEG PRD ccccccccccccchhhhhhhhhhhhhhhhhhhhcceeeeeeeccccchhhhhhhhhhhhh MEM

SEQ ASKEGMEAAVEKQRVREEILAKGGFLPPEEIKERFDSNCITPGTEFMDNLAKCLRYYIAD SEG PRD hhhhhhhhhhhhhhhhhhhhhhcccccccccccccccccccccchhhhhhhhhhhhhhhh

MEM

SEQ RLNNDPGWKNLTVILSDASAPGEGEHKIMDYIRRQRAQPNHDPNTHHCLCGADADLIMLG SEG PRD hcccccccceeeeeeeccccccccchhhhhhhhhhhhccccccccccccccccccceeec MEM

SEQ LATHEPNFTIIREEFKPNKPKPCGLCNQFGHEVKDCEGLPREKKGKHDELADSLPCAEGE SEG PRD ccccccccccccccccccccccceeeccccccccccccccchhhhhhhhhcccccccccc

MEM

SEQ FIFLRLNVLREYLERELTMASLPFTFDVERSIDDWVFMCFFVGNDFLPHLPSLEIRENAI SEG PRD ccchhhhhhhhhhhhhhhhhhhchhhhhhhhhhhheeeeeeccccccccccccccchhhh MEM MMMMMMMMMMMMMMMMMMM

SEQ DRLVNIYKNVVHKTGGYLTESGYVNLQRVQMIMLAVGEVEDSIFKKRKDDEDSFRRRQKE SEG xxxxxx PRD hhhhhhhhhhhcccccccccccchhhhhhhhhhhhhhcchhhhhhhhhhhhhhhhhhhhh MEM

SEQ KRKRMKRDQPAFTPΞGILTPHALGSRNSPGSQVASNPRQAAYEMRMQNNSSPΞISPNTSF SEG xxxxxxx xxxxxxxxxxxxx PRD hhhhhhhhcccccccccccccccccccccccchhhhhhhhhhhhhhhccccccccccccc

MEM

SEQ TSDGSPSPLGGIKRKAEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDAADEKFRRKVVQ SEG XX xxxxxxxxxxx PRD ccccccccchhhhhhhhcccccccchhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhh MEM

SEQ SYVEGLCWVLRYYYQGCASWKWYYPFHYAPFASDFEGIADMPSDFEKGTKPFKPLEQLMG SEG PRD hhhhhhheeeeeeccccccccccccccccccccccccccccccccccccccccchhhhhh

MEM

SEQ VFPAASGNFLPPSWRKLMSDPDSSIIDFYPEDFAIDLNGKKYAWQGVALLPFVDERRLRA SEG PRD hccccccccccccccccccccccceeeccccceeeccccccceeeeeeeeeccchhhhhh

MEM

SEQ ALEEVYPDLTPEETRRNSLGGDVLFVGKHHPLHDFILELYQTGΞTEPVEVPPELCHGIQG SEG PRD hhhhhccccchhhhhhcccccceeeeeecccchhhhhhhhhcccccceeecccccccccc MEM

SEQ KFSLDEEAILPDQIVCSPVPMLRDLTQNTVVSINFKDPQFAEDYIFKAVMLPGARKPAAV SEG PRD cccccceeecccceeeccccccccccccceeeeecccccchhhhheeeccccccccccee MEM

SEQ LKPSDWEKSSNGRQWKPQLGFNRDRRPVHLDQAAFRTLGHVMPRGSGTGIYSNAAPPPVT SEG PRD eccccccccccccccccccccccccccccchhhhhhhhhhcccccccccccccccccccc MEM

SEQ YQGNLYRPLLRGQAQIPKLMSNMRPQDSWRGPPPLFQQQRFDRGVGAEPLLPWNRMLQTQ SEG PRD cccccchhhhhcccchhhhhcccccccccccccccchhhhhccccccccccccchhhhhh

MEM

SEQ NAAFQPNQYQMLAGPGGYPPRRDDRGGRQGYPREGRKYPLPPPSGRYNWN SEG xxxxxxxxxxxxxxxxxxxx PRD hcccccccceeecccccccccccccccccccccccccccccccccccccc

MEM

Prosite for DKFZphtes3_2ml8.3

PS00001 190->194 ASN_GLYCOSYLATION PDOC00001 PS00001 247->251 ASN_GLYCOSYLATION PDOC00001 PΞ00001 468->472 ASN_GLYCOSYLATION PDOC00001 PS00001 477->481 ASN_GLYCOSYLATION PDOC00001 PS00002 826->830 GLYCOSAMINOGLYCAN PDOC00002 PS00004 675->679 CAMP_PHOSPHO_SITE PDOC00004 PS00005 11->14 PKC_PHOSPHO_SITE PDOC00005 PS00005 116->119 PKC_PHOSPHO_SITE PDOC00005 PS00005 413->416 PKC_PHOSPHO_SITE PDOC00005 PS00005 559->562 PKC_PHOSPHO_SITE PDOC00005 PS00005 613->616 PKC_PHOSPHO_SITE PDOC00005 PS00005 674->677 PKC_PHOSPHO_SITE PDOC00005 PS00005 868->871 PKC_PHOSPHO_SITE PDOC00005 PS00005 944->947 PKC_PHOSPHO_SITE PDOC00005 PS00006 63->67 CK2_PHOSPHO_SITE PDOC00006 PS00006 331->335 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 499->503 CK2_PHOSPHO_SITE PDOC00006 PS00006 501->505 CK2_PHOSPHO_SITE PDOC00006 PS00006 541->545 CK2_PHOSPHO_SITE PDOC00006 PS00006 573->577 CK2_PHOSPHO_SITE PDOC00006 PS00006 583->587 CK2_PHOSPHO_SITE PDOC00006 PS00006 619->623 CK2_PHOSPHO_SITE PDOC00006 PS00006 624->628 CK2_PHOSPHO_SITE PDOC00006 PS00006 670->674 CK2_PHOSPHO_SITE PDOC00006 PS00006 723->727 CK2_PHOSPHO_SITE PDOC00006 PS00006 784->788 CK2_PHOSPHO_SITE PDOC00006 PS00007 659->667 TYR_PHOSPHO_SITE PDOC00007 PS00008 125->131 MYRISTYL PDOC00008 PS00008 375->381 MYRISTYL PDOC00008 PS00008 450->456 MYRISTYL PDOC00008 PS00008 600->606 MYRISTYL PDOC00008 PS00008 825->831 MYRISTYL PDOC00008 PS00008 829->835 MYRISTYL PDOC00008 PS00008 926->932 MYRISTYL PDOC00008 PS00009 638->642 AMIDATION PDOC00009 PS00009 934->938 AMIDATION PDOC00009

(No Pfam data available for DKFZphtes3 2ml8.3)

DKFZphtes3_2m20

group: testes derived

DKFZphtes3_2m20 encodes a novel 183 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . group: unknown

DKFZphtes3_2m20 encodes a novel ammo acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . unknown

EST hits are only from testis or uterus librarys remaining intron m3 ' UTR see EST-BLAST

Sequenced by EMBL

Locus: unknown

Insert length: 1341 bp

Poly A stretch at pos. 1320, polyadenylation signal at pos. 1300

1 GCAATCCAGG AGCTGAATGG TAACTCTTCC ACAAGCGAAA ACTGTTCGTG

51 AATACAAGCA AAAGGCCCCC CAAGAGGACC CCTGATATGA TCCAGCAGCC

101 TCGGGCCCCG CTGGTGTTGG AGAAGGCTTC TGGTGAAGGA TTTGGCAAAA

151 CCGCCGCTAT TATACAGCTC GCTCCTAAAG CTCCTGTTGA CCTGTGTGAG

201 ACAGAGAAAC TGAGGGCAGC CTTCTTTGCA GTCCCGTTGG AAATGAGAGG

251 GTCCTTCCTG GTGCTGCTCC TGAGGGAATG CTTCCGAGAC CTGAGCTGGC

301 TGGCACTCAT CCATAGCGTC CGTGGGGAGG CGGGGCTGCT GGTGACGAGT

351 ATCGTCCCGA AGACCCCGTT TTTCTGGGCC ATGCACATCA CTGAGGCTCT

401 GCACCAGAAC ATGCAGGCTC TGTTTAGCAC CCTGGCTCAG GCGGAGGAGC

451 AGCAGCCCTA CCTGGAGGCT CCACCGTTAT GCGCGGGACT CGCTGTCTGG

501 CAGAGTACCA CCTGGGGGAT TATGGACACG CCTGGAACAG GTGTTGGGTG

551 CTGGACAGGG TGGACACCTG GGCTGTGGTC ATGTTCATTG ATTTTGGACA

601 GTTGGCCACC ATCCCTGTGC AGTCTCTGCG CCAGCTAGAC AGCGACGACT

651 TCTGGACCAT CCCACCCCTG ACTCAGCCAT TCATGCTGGA GAAAGACATT

701 TTGAGTTCGT ATGAGGTTGT CCATCGAATC CTCAAAGGGA AAATCACTGG

751 TGCTTTGAAC TCGGCGGTAA CTGCTCCTGC ATCTAACTTG GCTGTTGTCC

801 CTCCACTCCT GCCCTTGGGG TGTCTGCAGC AGGCTGCTGC CTAGGCCTGG

851 ACACATTGCA CATCCTAAAG TTTGAAGAGT CTAAATAACG GGGCTTCCCT

901 CAGCATGTTC CCTCTCCTGT TTGCCACGGA TCCAGAGCCA CCTGCCCTGT

951 CTTCTCGTAC CCCTTTCACT CTTGAGGCCT GGGAGGTGAA AAAGGCCAGA

1001 CTGTGCCCAG GATTGATTCA ATTTTGCTTT TACTCCCAGC TTCCCTCTCA

1051 AAAGAGAGTG AAGTCTCATT TGTCATGTGT CTTCAGTTCC CCAACTTGGC

1101 ATGAACATTT GAACCAAACA TAGGAAACTA CCATTAGGTT GAAAGCCTGA

1151 GGCAGCTGGG ATGGTCTTTC TTGTGTCTCT TCTTTGCACC CCAGAGCATG

1201 ATATAAGTGG TCCTAACAGA TTCTGGATAA TGGAGAAGCC CTCTGCTGGT

1251 TTTCCTGGCA TTCCATGTAG AATAGGTAGA GAATATTTAA CCAATGAGCA

1301 AATAAATGTT GGCATGTTTC ATGAAAAAAA AAAAAAAAAA A

BLAST Results

o BLAST result

Medlme entries o Medlme entry Peptide information for frame 2

ORF from 479 bp to 841 bp; peptide length: 121 Category: questionable ORF Classification: no clue

1 MRGTRCLAEY HLGDYGHAWN RCWVLDRVDT WAVVMFIDFG QLATIPVQSL 51 RQLDSDDFWT IPPLTQPFML EKDILSSYEV VHRILKGKIT GALNSAVTAP 101 ASNLAVVPPL LPLGCLQQAA A

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_2m20, frame 2 No Alert BLASTP hits found

Peptide information for frame 3

ORF from 87 bp to 635 bp; peptide length: 183 Category: putative protein Classification: no clue

1 MIQQPRAPLV LEKASGEGFG KTAAIIQLAP KAPVDLCETE KLRAAFFAVP

51 LEMRGSFLVL LLRECFRDLS WLALIHSVRG EAGLLVTΞIV PKTPFFWAMH

101 ITEALHQNMQ ALFSTLAQAE EQQPYLEAPP LCAGLAVWQS TTWGIMDTPG

151 TGVGCWTGWT PGLWSCSLIL DSWPPSLCSL CAS

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_2m20, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphtes3_2m20, frame 2

Report for DKFZphtes3_2m20.2

[LENGTH] 121

[MW] 13436.69

[pi] 5.81

[KW] Alpha Beta

SEQ MRGTRCLAEYHLGDYGHAWNRCWVLDRVDTWAVVMFIDFGQLATIPVQSLRQLDSDDFWT

PRD ccchhhhhcccccccccccceeeecccccccceeeeeecccccccccccccccccccccc

SEQ IPPLTQPFMLEKDILΞΞYEVVHRILKGKITGALNSAVTAPASNLAVVPPLLPLGCLQQAA

PRD cccccchhhhhhhcchhhhhhhhhhcccccchhhhhhcccccceeeeccccccccccccc

SEQ A

PRD c

(No Prosite data available for DKFZphtes3_2m20.2) (No Pfam data available for DKFZphtes3_2m20.2)

Pedant information for DKFZphtes3_2m20, frame 3

Report for DKFZphtes3_2m20.3

[LENGTH] 183

[MW] 19971.49

[pi] 5.31

[KW] Alpha Beta SEQ MIQQPRAPLVLEKAΞGEGFGKTAAIIQLAPKAPVDLCETEKLRAAFFAVPLEMRGSFLVL

PRD ccccccccceeeecccccccccccccccccccccchhhhhhhhhhhhhhhhhcchhhhhh

SEQ LLRECFRDLSWLALIHSVRGEAGLLVTSIVPKTPFFWAMHITEALHQNMQALFSTLAQAE

PRD hhhhhhcchhhhhhhhhhcccceeeeeeeccccchhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ EQQPYLEAPPLCAGLAVWQSTTWGIMDTPGTGVGCWTGWTPGLWSCSLILDSWPPSLCSL

PRD hhhcccccccccccceeeecccceeecccccccccccccccccccceeeeccccccceee

SEQ CAS

PRD CCC

(No Prosite data available for DKFZphtes3_2m20.3) (No Pfam data available for DKFZphtes3_2m20.3)

DKFZphtes3_2n9

group: testes derived

DKFZphtes3_2n9 encodes a novel 184 ammo acid protein with very weak similarity to Homo sapiens PAC clone DJ0771P04 from 7qll .21-qll .23.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . unknown on genomic level encoded by HS1186N24, no splice pattern but EST matches

Sequenced by EMBL

Locus : unknown

Insert length: 1000 bp

Poly A stretch at pos. 988, polyadenylation signal at pos. 970

1 CAACTTTTTA AAGATGTGAA TTGGACAGCC AGACTTGCTT ATTTGTCTGA

51 TATCTTCAGT ATTTTTTAAT GATCTTAATG CTTCTATGCA AGGGAAGAAT

101 GCAACTTATT TTTCAATGGC AGATAAAGTT GAAGGACAAA AACAGAAGTT

151 AGAAGCTTGG AAAAACAGAA TTTCTACAGA TTGTTATGAC ATGTTTCATA

201 ATTTAACAAC AATTATCAAT GAAGTAGGTA ATGATCTTGA TATTGCACAT

251 CTGCGAAAAG TTATCAGTGA ACATCTTACA AATTTGTTAG AATGTTTTGA

301 ATTTTATTTT CCATCAAAAG AAGATCCACG CATAGGAAAT TTGTGGATCC

351 AAAATCCATT TCTTTCATCA AAAGATAACT TAAATTTAAC TGTAACTCTA

401 CAGGATAAGT TGTTGAAGCT GGCTACCGAC GAAGGATTGA AAATCAGTTT

451 TGAAAATACA GCATCACTTC CTTCATTTTG GATAAAAGCT AAAAATGACT

501 ATCCTGAGCT TGCTGAGATT GCTTTAAAAT TGCTGCTTCT TTTCCCCTCA

551 ACATACCTCT GTGAGACCGG ATTCTCTACT TTAAGTGTTA TTAAAACAAA

601 ACATAGAAAC AGTTTAAATA TACATTATCC CCTGAGGTAG CATTGTCATC

651 AATCCAACCT AGATTAGACA AATTAACAAG CAAGAAGCAA GCTCACTTAT

701 CACATTAAAA GCTTTAAATA TTGATATGTA AGGTATTGGT TCAAAGTATG

751 CATATAAGCA TTGAGTGTGA GGAATTTGCT ATTTCACTTT AAACTTTCTG

801 TCTAGTTACA GTTATGGAAG TATGAGAAGT TATGAGTGAA ACAGCAATTT

851 TCTATATAAA TTGCCTATAT GTATATTTTC AATTAAGAAT GTGTACAGTT

901 TTTATAATTC TATTTTTCCT CATATTTGTC GTATTTATTA AAATATAATT

951 TTAAATCTGT TGATTCTAAT ATTAAAACAT TTGATCTTAA AAAAAAAAAA

BLAST Results

Entry HS1186N24 from database EMBLNEW:

Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone 1186N24

Score = 4921, P = 5.8e-215, identities = 989/992

Medline entries

No Medlme entry

Peptide information for frame 2

ORF from 86 bp to 637 bp; peptide length: 184 Category: similarity to unknown protein Classification: no clue

1 MQGKNATYFS MADKVEGQKQ KLEAWKNRIS TDCYDMFHNL TTIINEVGND

51 LDIAHLRKVI SEHLTNLLEC FEFYFPSKED PRIGNLWIQN PFLSSKDNLN

101 LTVTLQDKLL KLATDEGLKI SFENTASLPS FWIKAKNDYP ELAEIALKLL

151 LLFPSTYLCE TGFSTLSVIK TKHRNSLNIH YPLR

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_2n9, frame 2

TREMBLNEW:AC004883_3 gene: "WUGSC :H_DJ0771P04.2"; Homo sapiens PAC clone DJ0771P04 from 7qll .21-qll .23, complete sequence., N = 1, Score = 94, P = 0.042

>TREMBLNEW:AC004883_3 gene: "WUGSC :H_DJ0771P04.2"; Homo sapiens PAC clone DJ0771P04 from 7qll .21-qll .23, complete sequence. Length = 533

HSPs:

Score = 94 (14.1 bits), Expect = 4.3e-02, P = 4.2e-02 Identities = 39/177 (22%), Positives = 75/177 (42%)

Query: 1 MQGKNATYFSMADKVEGQKQKLEAWKNRISTDCYDMFHNLTTIINEVGNDLD-IAHLRKV 59

+QG + M D + KL W+ ++ + F L + L+ I + ++ Sbjct: 354 LQGHSQIVTQMYDLIRAFLAKLCLWETHLTRNNLAHFPTLKLASRNESDGLNYIPKIAEL 413

Query: 60 ISEHLTNLLECFEFYFPSKEDPRIGNLWIQNPFLSSKDNLNLTVTLQDKLLKLATDEGLK 119

+E L + F+ Y + + + +PF + D+++ LQ +++ L + LK Sbjct: 414 KTEFQKRLSD-FKLY ESELTL FSSPFSTKIDSVH—EELQMEVIDLQCNTVLK 4_63

Query: 120 ISFENTASLPSFWIKAKNDYPXXXXXXXXXXXXFPSTYLCETGFSTLSVIKTKHRNSL 177

++ +P F+ YP F STY+CE FS + + KTK+ + L

Sbjct: 464 TKYDKVG-IPEFYKYLWGSYPKYKHHCAKILSMFGSTYICEQLFSIMKLSKTKYCSQL 520

Pedant information for DKFZphtes3_2n9, frame 2

Report for DKFZphtes3_2n9.2

[LENGTH] 184

[MW] 21203.53

[pi] 6.52

[KW] Alpha_Beta

[KW] LOW_COMPLEXITY 6.52 %

SEQ MQGKNATYFSMADKVEGQKQKLEAWKNRISTDCYDMFHNLTTIINEVGNDLDIAHLRKVI SEG

PRD ccccccchhhhhhhhhhhhhhhhhhhhhhcchhhhhcccceeecccccccchhhhhhhhh

SEQ SEHLTNLLECFEFYFPSKEDPRIGNLWIQNPFLSSKDNLNLTVTLQDKLLKLATDEGLKI SEG

PRD hhhhhhhhhhhhcccccccccccceeeeccccccccccceeeeehhhhhhhhhhhcccee

SEQ SFENTASLPSFWIKAKNDYPELAEIALKLLLLFPSTYLCETGFSTLSVIKTKHRNSLNIH SEG xxxxxxxxxxxx

PRD eecccccccceeeeecccchhhhhhhhhhhhhcccccccccccceeeeeecccccceeec

SEQ YPLR SEG .... PRD cccc

(No Prosite data available for DKFZphtes3_2n9.2) (No Pfam data available for DKFZphtes3_2n9.2) DKFZphtes3_30f4

group: testes derived

DKFZphtes3_30f4 encodes a novel 192 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . unknown

Sequenced by LMU

Locus: /map="717.2-8 cR from top of Chr8 linkage group"

Insert length: 1388 bp

Poly A stretch at pos. 1330, polyadenylation signal at pos. 1310

1 CACTGAGCCC TCCTCAGATG GTTAGTGGCT TCCAACAGCC ATCAGGAGTG

51 TTTCTTGAAT GCCCCAGGTG TGGAGGACTT GGTCTGTGAC CACCTAGAAC

101 CCCAGAGCTG AACAGGAAGC CGTCCCTGCA GCAACAAGAG GGCTGGAAGG

151 GGGAGCTGCA GGCCACCCTC GGCTCTCCCA CTGCTGGGGC GGTGATGTTC

201 GGGTGACATG TTTGAAAAAT ACTCTTAAAG ATACCAACTG TTCCCTTATA

251 TGGCTAATGG TTTGTGCAGC CACCAGCGAT GGCGGCCCCT ATTAGAGACC

301 AGGTTTGTTA AAACACCAAA TATTGCTGTC CACACTAGAC ATTAACCGGC

351 TTCAGAAAAG ATGGACACCT TTTCCCACGC TGTTTCGCTT CTTAACTTTG

401 GTCCAGCTTT AGCCACCACA CAGCGTGTGA GGGACTGCTG CTGCGGAGTC

451 AGCCTCGTTT GTCCCTCCGC CTCCCACCAG CATGCGCCGC TTCTGAGAGA

501 CACCAGCTCC CTGCCTCCAA GCCTGGTGCC ACAGGCCTGT CGTGAGGGAC

551 CCCTGCTTCC GAGAGCTCCT GGGGGGGTTC TGCCCTTCAC CACCTGGGAG

601 AGGTGTCAGT TCAGTTCCGA GTTGAACAAG GCCCGTGCAC ACAGCATGTT

651 GGGGGCCCAG CCCAAAGTTC TTGTCACCTC CTCATGCAAA GCCAGCCATC

701 ACCCTCCGGC CAGAGCTCAA GGTGGCCCCT TGGCCAGCCC CTCCTTGGGT

751 CCTCCAGGAG GACTGAGCAC CCCTCCTAGC GGCATCCCTT GCCCTCCACA

801 GTGCTGCCAG GGGCACGTCG CTCTGTGCCG TGGACTGAGA CCATCCCCTG

851 GTGACAGAAT GACCCGTTTG TTGGAAATGC CTCGTTGCCA GAGAAACTCC

901 CCAGGCATCT CGGAACGAAA CTATTTAGTT CCATTGTGAA CTGGCCACGG

951 GACAGCTTTT TATCAACTTA TTAAGTTGGA GCACTGTAAT CGCGCTTGCT

1001 GAGTTAGCAG TGGTGGTAAG CGTGTGTTAA ACACATAATG TTACGTTTTA

1051 GGAGAGAGAG GTCGTAAGGA AGTGTCGTGT CGCTCATGAC TCTCTTCTAT

1101 TAGTTGGGTA ACAGTGGCCT CATGTTTGTG TCTGTGTGTA CACAGAGCCC

1151- TTAGGTTCTG CTCTGTTTCT TTGCCAGGTG AATGTTTGTG GCATGCGCTG

1201 CTGTCCGCGC CCCTCTGTCC TGCGCAGGGT TCAGCTGTGC GGCGCCCTGA

1251 TTTCCTCCAT GCACACAGAA CCTCCTTGTG TCTGTTTCTC TGTTCCTCTG

1301 TGGCTGACTC AATAAACTTT TCCCTCTGAC ATGAAAAAAA AAAAAAAAAA

1351 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAG

BLAST Results

Entry HS548358 from database EMBL: human STS EST67250. Score = 2126, P = 1.5e-89, identities = 444/472

Entry HS670351 from database EMBL: human STS WI-18501. Score = 2089, P = 7.1e-88, identities = 445/476

Medlme entries

No Medline entry

Peptide information for frame 1

ORF from 361 bp to 936 bp; peptide length: 192 Category: putative protein Classification: no clue 1 MDTFSHAVSL LNFGPALATT QRVRDCCCGV SLVCPSASHQ HAPLLRDTSS 51 LPPSLVPQAC REGPLLPRAP GGVLPFTTWE RCQFΞSELNK ARAHSMLGAQ 101 PKVLVTSSCK ASHHPPARAQ GGPLASPSLG PPGGLSTPPS GIPCPPQCCQ 151 GHVALCRGLR PSPGDRMTRL LEMPRCQRNS PGISERNYLV PL

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_30f4, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphtes3_30f4, frame 1

Report for DKFZphtes3_30f4.1

[LENGTH] 192

[MW] 20281.56

[pi] 9.21

[BLOCKS] BL01013C Oxysterol-binding protein family proteins

[KW] All_Alpha

[KW] LOW COMPLEXITY 10.94 %

SEQ MDTFSHAVSLLNFGPALATTQRVRDCCCGVSLVCPSASHQHAPLLRDTSSLPPSLVPQAC

SEG

PRD ccchhhhheeecccccchhhhhhhhcccceeeeccccccccccccccccccccccccccc

SEQ REGPLLPRAPGGVLPFTTWERCQFSSELNKARAHSMLGAQPKVLVTSSCKASHHPPARAQ

SEG

PRD cccccccccccccccccccccchhhhhhhhhhhhhhccccceeeeecccccccccccccc

SEQ GGPLASPSLGPPGGLSTPPSGIPCPPQCCQGHVALCRGLRPSPGDRMTRLLEMPRCQRNS

SEG xxxxxxxxxxxxxxxxxxxxx

PRD cccccccccccccccccccccccccccccchhhhhhhhcccccccchhhhhccccccccc

SEQ PGISERNYLVPL

SEG

PRD cccccccccccc

(No Prosite data available for DKFZphtes3_30f4.1) (No Pfam data available for DKFZphtes3_30f4.1)

DKFZphtes3_35b4

group: cell cycle

DKFZphtes3_35b4 encodes a novel 1780 amino acid protein which is C-terminal identical to human M-phase phosphoprotem-1 (MPPl).

The novel protein contains a N-termmal Pfam kinesm motor domain and a ATP/GTP-bindmg site motif A (P-loop). MPPl is expressed and phosphorylated in the metaphase. Therefore the novel protein seems to be involved in the mitotic spindle during cell division.

The new protein can find application in modulation of the mitotic spindle.

"M-phase phosphoproteιn-1" extension motor protein

Sequenced by DKFZ

Locus: /map="750_H_l; 758_H_7; 759_C_9; 8 7_D_4; 906_D_1; 931_D_3; 944_C_1; 750_G_12; 800_A_11; 512.1 cR from top of ChrlO linkage group"

Insert length: 6284 bp

No poly A stretch found, no polyadenylation signal found

1 ATCGCAGTGC TGCTCGCGGG TCTGGCTAGT CAGGCGAAGT TTGCAGAATG 51 GAATCTAATT TTAATCAAGA GGGAGTACCT CGACCATCTT ATGTTTTTAG

101 TGCTGACCCA ATTGCAAGGC CTTCAGAAAT AAATTTCGAT GGCATTAAGC

151 TTGATCTGTC TCATGAATTT TCCTTAGTTG CTCCAAATAC TGAGGCAAAC

201 AGTTTCGAAT CTAAAGATTA TCTCCAGGTT TGTCTTCGAA TAAGACCATT

251 TACACAGTCA GAAAAAGAAC TTGAGTCTGA GGGCTGTGTG CATATTCTGG

301 ATTCACAGAC TGTTGTGCTG AAAGAGCCTC AATGCATCCT TGGTCGGTTA

351 AGTGAAAAAA GCTCAGGGCA GATGGCACAG AAATTCAGTT TTTCCAAGGT

401 TTTTGGCCCA GCAACTACAC AGAAGGAATT CTTTCAGGGT TGCATTATGC

451 AACCAGTAAA AGACCTCTTG AAAGGACAGA GTCGTCTGAT TTTTACTTAC

501 GGGCTAACCA ATTCAGGAAA AACATATACA TTTCAAGGGA CAGAAGAAAA

551 TATTGGCATT CTGCCTCGAA CTTTGAATGT ATTATTTGAT AGTCTTCAAG

601 AAAGACTGTA TACAAAGATG AACCTTAAAC CACATAGATC CAGAGAATAC

651 TTAAGGTTAT CATCAGAACA AGAGAAAGAA GAAATTGCTA GCAAAAGTGC

701 ATTGCTTCGG CAAATTAAAG AGGTTACTGT GCATAATGAT AGTGATGATA

751 CTCTTTATGG AAGTTTAACT AACTCTTTGA ATATCTCAGA GTTTGAAGAA

801 TCCATAAAAG ATTATGAACA AGCCAACTTG AATATGGCTA ATAGTATAAA

851 ATTTTCTGTG TGGGTTTCTT TCTTTGAAAT TTACAATGAA TATATTTATG

901 ACTTATTTGT TCCTGTATCA TCTAAATTCC AAAAGAGAAA GATGCTGCGC

951 CTTTCCCAAG ACGTAAAGGG CTATTCTTTT ATAAAAGATC TACAATGGAT 1001 TCAAGTATCT GATTCCAAAG AAGCCTATAG ACTTTTAAAA CTAGGAATAA 1051 AGCACCAGAG TGTTGCCTTC ACAAAATTGA ATAATGCTTC CAGTAGAAGT 1101 CACAGCATAT TCACTGTTAA AATATTACAG ATTGAAGATT CTGAAATGTC 1151 TCGTGTAATT CGAGTCAGTG AATTATCTTT ATGTGATCTT GCTGGTTCAG 1201 AACGAACTAT GAAGACACAG AATGAAGGTG AAAGGTTAAG AGAGACTGGG 1251 AATATCAACA CTTCTTTATT GACTCTGGGA AAGTGTATTA ACGTCTTGAA 1301 GAATAGTGAA AAGTCAAAGT TTCAACAGCA TGTGCCTTTC CGGGAAAGTA 1351 AACTGACTCA CTATTTTCAA AGTTTTTTTA ATGGTAAAGG GAAAATTTGT 1401 ATGATTGTCA ATATCAGCCA ATGTTATTTA GCCTATGATG AAACACTCAA 1451 TGTATTGAAG TTCTCCGCCA TTGCACAAAA AGTTTGTGTC CCAGACACTT 1501 TAAATTCCTC TCAAGATAAA TTATTTGGAC CTGTCAAATC TTCTCAAGAT 1551 GTATCACTAG ACAGTAATTC AAACAGTAAA ATATTAAATG TAAAAAGAGC 1601 CACCATTTCA TGGGAAAATA GTCTAGAAGA TTTGATGGAA GACGAGGATT 1651 TGGTTGAGGA GCTAGAAAAC GCTGAAGAAA CTCAAAATGT GGAAACTAAA 1701 CTTCTTGATG AAGATCTAGA TAAAACATTA GAGGAAAATA AGGCTTTCAT 1751 TAGCCACGAG GAGAAAAGAA AACTGTTGGA CTTAATAGAA GACTTGAAAA 1801 AAAAACTGAT AAATGAAAAA AAGGAAAAAT TAACCTTGGA ATTTAAAATT 1851 CGAGAAGAAG TTACACAGGA GTTTACTCAG TATTGGGCTC AACGGGAAGC 1901 TGACTTTAAG GAGACTCTGC TTCAAGAACG AGAGATATTA GAAGAAAATG 1951 CTGAACGTCG TTTGGCTATC TTCAAGGATT TGGTTGGTAA ATGTGACACT 2001 CGAGAAGAAG CAGCGAAAGA CATTTGTGCC ACAAAAGTTG AAACTGAAGA 2051 AGCTACTGCT TGTTTAGAAC TAAAGTTTAA TCAAATTAAA GCTGAATTAG 2101 CTAAAACCAA AGGAGAATTA ATCAAAACCA AAGAAGAGTT AAAAAAGAGA 2151 GAAAATGAAT CAGATTCATT GATTCAAGAG CTTGAGACAT CTAATAAGAA 2201 AATAATTACA CAGAATCAAA GAATTAAAGA ATTGATAAAT ATAATTGATC 2251 AAAAAGAAGA TACTATCAAC GAATTTCAGA ACCTAAAGTC TCATATGGAA 2301 AACACATTTA AATGCAATGA CAAGGCTGAT ACATCTTCTT TAATAATAAA 2351 CAATAAATTG ATTTGTAATG AAACAGTTGA AGTACCTAAG GACAGCAAAT 2401 CTAAAATCTG TTCAGAAAGA AAAAGAGTAA ATGAAAATGA ACTTCAGCAA 2451 GATGAACCAC CAGCAAAGAA AGGGTCTATC CATGTTAGTT CAGCTATCAC 2501 TGAAGACCAA AAGAAAAGTG AAGAAGTGCG ACCGAACATT GCAGAAATTG 2551 AAGACATCAG AGTTTTACAA GAAAATAATG AAGGACTGAG AGCATTTTTA 2601 CTCACTATTG AGAATGAACT TAAAAATGAA AAGGAAGAAA AAGCAGAATT

2651 AAATAAACAG ATTGTTCATT TTCAGCAGGA ACTTTCTCTT TCTGAAAAAA 2701 AGAATTTAAC TTTAAGTAAA GAGGTCCAAC AAATTCAGTC AAATTATGAT 2751 ATTGCAATTG CTGAATTACA TGTGCAGAAA AGTAAAAATC AAGAACAGGA 2801 GGAAAAGATC ATGAAATTGT CAAATGAGAT AGAAACTGCT ACAAGAAGCA 2851 TTACAAATAA TGTTTCACAA ATAAAATTAA TGCACACGAA AATAGACGAA 2901 CTACGTACTC TTGATTCAGT TTCTCAGATT TCAAACATAG ATTTGCTCAA 2951 TCTCAGGGAT CTGTCAAATG GTTCTGAGGA GGATAATTTG CCAAATACAC 3001 AGTTAGACCT TTTAGGTAAT GATTATTTGG TAAGTAAGCA AGTTAAAGAA 3051 TATCGAATTC AAGAACCCAA TAGGGAAAAT TCTTTCCACT CTAGTATTGA 3101 AGCTATTTGG GAAGAATGTA AAGAGATTGT GAAGGCCTCT TCCAAAAAAA 3151 GTCATCAGAT TGAGGAACTG GAACAACAAA TTGAAAAATT GCAGGCAGAA 3201 GTAAAAGGCT ATAAGGATGA AAACAATAGA CTAAAGGAGA AGGAGCATAA 3251 AAACCAAGAT GACCTACTAA AAGAAAAAGA AACTCTTATA CAGCAGCTGA 3301 AAGAAGAATT GCAAGAAAAA AATGTTACTC TTGATGTTCA AATACAGCAT 3351 GTAGTTGAAG GAAAGAGAGC GCTTTCAGAA CTTACACAAG GTGTTACTTG 3401 CTATAAGGCA AAAATAAAGG AACTTGAAAC AATTTTAGAG ACTCAGAAAG 3451 TTGAACGTAG TCATTCAGCC AAGTTAGAAC AAGACATTTT GGAAAAGGAA 3501 TCTATCATCT TAAAGCTAGA AAGAAATTTG AAGGAATTTC AAGAACATCT 3551 TCAGGATTCT GTCAAAAACA CCAAAGATTT AAATGTAAAG GAACTCAAGC

3601 TGAAAGAAGA AATCACACAG TTAACAAATA ATTTGCAAGA TATGAAACAT

3651 TTACTTCAAT TAAAAGAAGA AGAAGAAGAA ACCAACAGGC AAGAAACAGA 3701 AAAATTGAAA GAGGAACTCT CTGCAAGCTC TGCTCGTACC CAGAATCTGA 3751 AAGCAGATCT TCAGAGGAAG GAAGAAGATT ATGCTGACCT GAAAGAGAAA 3801 CTGACTGATG CCAAAAAGCA GATTAAGCAA GTACAGAAAG AGGTATCTGT 3851 AATGCGTGAT GAGGATAAAT TACTGAGGAT TAAAATTAAT GAACTGGAGA 3901 AAAAGAAAAA CCAGTGTTCT CAGGAATTAG ATATGAAGCA GCGAACCATT 3951 CAGCAACTCA AGGAGCAGTT AAATAATCAG AAAGTGGAAG AAGCTATACA 4001 ACAGTATGAG AGAGCATGCA AAGATCTAAA TGTTAAAGAG AAAATAATTG 4051 AAGACATGCG AATGACACTA GAAGAACAGG AACAAACTCA GGTAGAACAG 4101 GATCAAGTGC TTGAGGCTAA ATTAGAGGAA GTTGAAAGGC TGGCCACAGA 4151 ATTGGAAAAA TGGAAGGAAA AATGCAATGA TTTGGAAACC AAAAACAATC 4201 AAAGGTCAAA TAAAGAACAT GAGAACAACA CAGATGTGCT TGGAAAGCTC 4251 ACTAATCTTC AAGATGAGTT ACAGGAGTCT GAACAGAAAT ATAATGCTGA 4301 TAGAAAGAAA TGGTTAGAAG AAAAAATGAT GCTTATCACT CAAGCGAAAG 4351 AAGCAGAGAA TATACGAAAT AAAGAGATGA AAAAATATGC TGAGGACAGG

4401 GAGCGTTTTT TTAAGCAACA GAATGAAATG GAAATACTGA CAGCCCAGCT 4451 GACAGAGAAA GATAGTGACC TTCAAAAGTG GCGAGAAGAA CGAGATCAAC 4501 TGGTTGCAGC TTTAGAAATA CAGCTAAAAG CACTGATATC CAGTAATGTA 4551 CAGAAAGATA ATGAAATTGA ACAACTAAAA AGGATCATAT CAGAGACTTC 4601 TAAAATAGAA ACACAAATCA TGGATATCAA GCCCAAACGT ATTAGTTCAG 4651 CAGATCCTGA CAAACTTCAA ACTGAACCTC TATCGACAAG TTTTGAAATT 4701 TCCAGAAATA AAATAGAGGA TGGATCTGTA GTCCTTGACT CTTGTGAAGT 4751 GTCAACAGAA AATGATCAAA GCACTCGATT TCCAAAACCT GAGTTAGAGA 4801 TTCAATTTAC ACCTTTACAG CCAAACAAAA TGGCAGTGAA ACACCCTGGT 4851 TGTACCACAC CAGTGACAGT TGAGATTCCC AAGGCTCGGA AGAGGAAGAG 4901 TAATGAAATG GAGGAGGACT TGGTGAAATG TGAAAATAAG AAGAATGCTA 4951 CACCCAGAAC TAATTTGAAA TTTCCTATTT CAGATGATAG AAATTCTTCT 5001 GTCAAAAAGG AACAAAAGGT TGCCATACGT CCATCATCTA AGAAAACATA 5051 TTCTTTACGG AGTCAGGCAT CCATAATTGG TGTAAACCTG GCCACTAAGA 5101 AAAAAGAAGG AACACTACAG AAATTTGGAG ACTTCTTACA ACATTCTCCC 5151 TCAATTCTTC AATCAAAAGC AAAGAAGATA ATTGAAACAA TGAGCTCTTC 5201 AAAGCTCTCA AATGTAGAAG CAAGTAAAGA AAATGTGTCT CAACCAAAAC 5251 GAGCCAAACG GAAATTATAC ACAAGTGAAA TTTCATCTCC TATTGATATA 5301 TCAGGCCAAG TGATTTTAAT GGACCAGAAA ATGAAGGAGA GTGATCACCA 5351 GATTATCAAA CGACGACTTC GAACAAAAAC AGCCAAATAA ATCACTTATG 5401 GAAATGTTTA ATATAAATTT TATAGTCATA GTCATTGGAA CTTGCATCCT 5451 GTATTGTAAA TATAAATGTA TATATTATGC ATTAAATCAC TCTGCATATA 5501 GATTGCTGTT TTATACATAG TATAATTTTA ATTCAATAAA TGAGTCAAAA 5551 TTTGTATATT TTTATAAGGC TTTTTTATAA TAGCTTCTTT CAAACTGTAT 5601 TTCCCTATTA TCTCAGACAT TGGATCAGTG AAGATCCTAG GAAAGAGGCT 5651 GTTATTCTCA TTTATTTTGC TATACAGGAT GTAATAGGTC AGGTATTTGG 5701 TTTACTTATA TTTAACAATG TCTTATGAAT TTTTTTTACT TTATCTGTTA 5751 TACAACTGAT TTTACATATC TGTTTGGATT ATAGCTAGGA TTTGGAGAAT 5801 AAGTGTGTAC AGATCACAAA ACATGTATAT ACATTATTTA GAAAAGATCT 5851 CAAGTCTTTA ATTAGAATGT CTCACTTATT TTGTAAACAT TTTGTGGGTA 5901 CATAGTACAT GTATATATTT ACGGGGTATG TGAGATGTTT TGACACAGGC 5951 ATGCAATGTG AAATACGTGT ATCATGGAGA ATGAGGTATC CATCCCCTCA 6001 AGCATTTTTC CTTTGAATTA CAGATAATCC AATTACATTC TTTAGATCAT 6051 TTAAAAATAT ACAAGTAAGT TATTATTGAT TATAGTCACT CTATTGTGCT 6101 ATCAGATAGT AGATCATTCT TTTTATCTTA TTTGTTTTTG TACCCATTAA 6151 CCATCCCCAC CTCCCCCTGC AACCGTCAGT ACCCTTACCA GCCACTGGTA 6201 ACCATTCTTC TACTCTGTAT GCCCATGAGG TCAATTGATT TTATTTTTAG 6251 ATCCCATAAA TAAATGAGAA CATGCAAAAA AAAA

BLAST Results

Entry HS898149 from database EMBL: human STS WI-9217. Score = 4247, P = 1.5e-187, identities = 855/862

Medline entries

94119956:

Cloning of cDNAs for M-phase phosphoproteins recognized by the MPM2 monoclonal antibody and determination of the phosphorylated epitope.

98101856:

Interaction of a Golgi-associated kmesin-like protein with

Rab6.

95122643:

Identification and partial characterization of mitotic centromere-associated kmesin, a kinesin-related protein that associates with centromeres during mitosis .

Peptide information for frame 3

ORF from 48 bp to 5387 bp; peptide length: 1780 Category: known protein

Classification: Cell structure/motility Prosite motifs: ATP GTP A (152-160)

1 MESNFNQEGV PRPSYVFSAD PIARPSEINF DGIKLDLSHE FSLVAPNTEA

51 NSFESKDYLQ VCLRIRPFTQ SEKELESEGC VHILDSQTVV LKEPQCILGR

101 LSEKSSGQMA QKFSFSKVFG PATTQKEFFQ GCIMQPVKDL LKGQSRLIFT

151 YGLTNSGKTY TFQGTEENIG ILPRTLNVLF DΞLQERLYTK MNLKPHRSRE

201 YLRLSSEQEK EEIASKSALL RQIKEVTVHN DSDDTLYGSL TNSLNISEFE

251 ESIKDYEQAN LNMANSIKFS VWVSFFEIYN EYIYDLFVPV SSKFQKRKML

301 RLSQDVKGYS FIKDLQWIQV SDSKEAYRLL KLGIKHQΞVA FTKLNNASSR

351 SHSIFTVKIL QIEDSEMSRV IRVSELSLCD LAGSERTMKT QNEGERLRET

401 GNINTΞLLTL GKCINVLKNS EKSKFQQHVP FRESKLTHYF QSFFNGKGKI

451 CMIVNISQCY LAYDETLNVL KFSAIAQKVC VPDTLNSSQD KLFGPVKSSQ

501 DVSLDSNSNS KILNVKRATI SWENSLEDLM EDEDLVEELE NAEETQNVET

551 KLLDEDLDKT LEENKAFISH EEKRKLLDLI EDLKKKLINE KKEKLTLEFK

601 IREEVTQEFT QYWAQREADF KETLLQEREI LEENAERRLA IFKDLVGKCD

651 TREEAAKDIC ATKVETEEAT ACLELKFNQI KAELAKTKGE LIKTKEELKK

701 RENESDSLIQ ELETSNKKII TQNQRIKELI NIIDQKEDTI NEFQNLKSHM

751 ENTFKCNDKA DTSSLIINNK LICNETVEVP KDSKSKICSE RKRVNENELQ

801 QDEPPAKKGS IHVSSAITED QKKSEEVRPN IAEIEDIRVL QENNEGLRAF

851 LLTIENELKN EKEEKAELNK QIVHFQQELS LSEKKNLTLS KEVQQIQSNY

901 DIAIAELHVQ KΞKNQEQEEK IMKLSNEIET ATRSITNNVS QIKLMHTKID

951 ELRTLDSVSQ ISNIDLLNLR DLSNGSEEDN LPNTQLDLLG NDYLVSKQVK

1001 EYRIQEPNRE NSFHSSIEAI WEECKEIVKA SΞKKSHQIEE LEQQIEKLQA

1051 EVKGYKDENN RLKEKEHKNQ DDLLKEKETL IQQLKEELQE KNVTLDVQIQ

1101 HVVEGKRALS ELTQGVTCYK AKIKELETIL ETQKVERSHS AKLEQDILEK

1151 ESIILKLERN LKEFQEHLQD SVKNTKDLNV KELKLKEEIT QLTNNLQDMK

1201 HLLQLKEEEE ETNRQETEKL KEELSAΞSAR TQNLKADLQR KEEDYADLKE

1251 KLTDAKKQIK QVQKEVSVMR DEDKLLRIKI NELEKKKNQC SQELDMKQRT

1301 IQQLKEQLNN QKVEEAIQQY ERACKDLNVK EKIIEDMRMT LEEQEQTQVE

1351 QDQVLEAKLE EVERLATELE KWKEKCNDLE TKNNQRSNKE HENNTDVLGK

1401 LTNLQDELQE SEQKYNADRK KWLEEKMMLI TQAKEAENIR NKEMKKYAED

1451 RERFFKQQNE MEILTAQLTE KDSDLQKWRE ERDQLVAALE IQLKALISSN

1501 VQKDNEIEQL KRIIΞETSKI ETQIMDIKPK RISSADPDKL QTEPLSTSFE

1551 ISRNKIEDGS VVLDSCEVST ENDQSTRFPK PELEIQFTPL QPNKMAVKHP

1601 GCTTPVTVEI PKARKRKSNE MEEDLVKCEN KKNATPRTNL KFPISDDRNS

1651 ΞVKKEQKVAI RPSSKKTYSL RSQASIIGVN LATKKKEGTL QKFGDFLQHS

1701 PSILQSKAKK IIETMSSSKL SNVEASKENV SQPKRAKRKL YTSEISSPID

1751 IΞGQVILMDQ KMKESDHQII KRRLRTKTAK

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_35b4, frame 3

TREMBL :U93121_1 product: "M-phase phosphoproteιn-1"; Human M-phase phosphoproteιn-1 mRNA, partial eds., N = 1, Score = 3743, P = 0 PIR:A36881 MPM2-reactιve phosphoprotein 1 - human (fragment), N = 2, Score = 2808, P = 2.5e-294

TREMBL:AF070672_1 product: "rabkinesinδ" ; Homo sapiens rabkmesinδ mRNA, complete eds., N = 2, Score = 680, P = 2.6e-99

>TREMBL:U93121_1 product: "M-phase phosphoproteιn-1"; Human M-phase phosphoproteιn-1 mRNA, partial eds. Length = 753

HSPs:

Score = 3743 (561.6 bits), Expect = O.Oe+00, P = O.Oe+00 Identities = 752/753 (99%), Positives = 753/753 (100%)

Query: 1028 VKASSKKSHQIEELEQQIEKLQAEVKGYKDENNRLKEKEHKNQDDLLKEKETLIQQLKEE 1087

VKASΞKKΞHQIEELEQQIEKLQAEVKGYKDENNRLKEKEHKNQDDLLKEKETLIQQLKEE Sbjct: 1 VKASSKKSHQIEELEQQIEKLQAEVKGYKDENNRLKEKEHKNQDDLLKEKETLIQQLKEE 60

Query: 1088 LQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKVERSHSAKLEQDI 1147

LQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKVERSHSAKLEQDI Sbjct: 61 LQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKVERΞHSAKLEQDI 120

Query: 1148 LEKESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKLKEEITQLTNNLQDMKHLLQLKE 1207

LEKESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKLKEEITQLTNNLQDMKHLLQLKE Sbjct: 121 LEKESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKLKEEITQLTNNLQDMKHLLQLKE 180

Query: 1208 EEEETNRQETEKLKEELSASSARTQNLKADLQRKEEDYADLKEKLTDAKKQIKQVQKEVS 1267

EEEETNRQETEKLKEELSASSARTQNLKADLQRKEEDYADLKEKLTDAKKQIKQVQKEVS Sbjct: 181 EEEETNRQETEKLKEELSASSARTQNLKADLQRKEEDYADLKEKLTDAKKQIKQVQKEVS 240

Query: 1268 VMRDEDKLLRIKINELEKKKNQCSQELDMKQRTIQQLKEQLNNQKVEEAIQQYERACKDL 1327

VMRDEDKLLRIKINELEKKKNQCSQELDMKQRTIQQLKEQLNNQKVEEAIQQYERACKDL Sbjct: 241 VMRDEDKLLRIKINELEKKKNQCSQELDMKQRTIQQLKEQLNNQKVEEAIQQYERACKDL 300

Query: 1328 NVKEKIIEDMRMTLEEQEQTQVEQDQVLEAKLEEVERLATELEKWKEKCNDLETKNNQRS 1387

NVKEKIIEDMRMTLEEQEQTQVEQDQVLEAKLEEVERLATELEKWKEKCNDLETKNNQRS Sbjct: 301 NVKEKIIEDMRMTLEEQEQTQVEQDQVLEAKLEEVERLATELEKWKEKCNDLETKNNQRS 360

Query: 1388 NKEHENNTDVLGKLTNLQDELQESEQKYNADRKKWLEEKMMLITQAKEAENIRNKEMKKY 1447

NKEHENNTDVLGKLTNLQDELQESEQKYNADRKKWLEEKMMLITQAKEAENIRNKEMKKY Sbjct: 361 NKEHENNTDVLGKLTNLQDELQESEQKYNADRKKWLEEKMMLITQAKEAENIRNKEMKKY 420

Query: 1448 AEDRERFFKQQNEMEILTAQLTEKDSDLQKWREERDQLVAALEIQLKALISSNVQKDNEI 1507

AEDRERFFKQQNEMEILTAQLTEKDSDLQKWREERDQLVAALEIQLKALISSNVQKDNEI Sbjct: 421 AEDRERFFKQQNEMEILTAQLTEKDSDLQKWREERDQLVAALEIQLKALISSNVQKDNEI 480

Query: 1508 EQLKRIISETΞKIETQIMDIKPKRISSADPDKLQTEPLSTSFEISRNKIEDGSVVLDSCE 1567

EQLKRIISETSKIETQIMDIKPKRISSADPDKLQTEPLSTSFEISRNKIEDGSVVLDSCE Sbjct: 481 EQLKRIISETSKIETQIMDIKPKRISSADPDKLQTEPLSTSFEISRNKIEDGSVVLDSCE 540

Query: 1568 VSTENDQSTRFPKPELEIQFTPLQPNKMAVKHPGCTTPVTVEIPKARKRKSNEMEEDLVK 1627

VSTENDQSTRFPKPELEIQFTPLQPNKMAVKHPGCTTPVTV+IPKARKRKSNEMEEDLVK Sbjct: 541 VSTENDQSTRFPKPELEIQFTPLQPNKMAVKHPGCTTPVTVKIPKARKRKSNEMEEDLVK 600

Query: 1628 CENKKNATPRTNLKFPISDDRNSΞVKKEQKVAIRPSSKKTYSLRSQASIIGVNLATKKKE 1687

CENKKNATPRTNLKFPISDDRNSSVKKEQKVAIRPSSKKTYSLRΞQASIIGVNLATKKKE Sbjct: 601 CENKKNATPRTNLKFPISDDRNSSVKKEQKVAIRPSSKKTYSLRSQASIIGVNLATKKKE 660

Query: 1688 GTLQKFGDFLQHSPSILQSKAKKIIETMSSSKLSNVEAΞKENVSQPKRAKRKLYTSEISS 1747

GTLQKFGDFLQHSPSILQSKAKKIIETMSSSKLSNVEASKENVSQPKRAKRKLYTSEISS Sbjct: 661 GTLQKFGDFLQHSPSILQSKAKKIIETMSSSKLSNVEASKENVSQPKRAKRKLYTΞEISS 720

Query: 1748 PIDISGQVILMDQKMKESDHQIIKRRLRTKTAK 1780

PIDIΞGQVILMDQKMKESDHQIIKRRLRTKTAK Sbjct: 721 PIDISGQVILMDQKMKESDHQIIKRRLRTKTAK 753

Score = 197 (29.6 bits), Expect = 2. le-11, P = 2. le-11 Identities = 114/542 (21%), Positives = 253/542 (46%)

Query: 692 IKTKEELKKRENESDSLIQELETSNKKIITQNQRIKELINIIDQKEDTINEFQNLKSHM- 750

+K + + E + I++L+ K +N R+KE + ++D + E + L + Sbjct: 1 VKASSKKSHQIEELEQQIEKLQAEVKGYKDENNRLKEKEH—KNQDDLLKEKETLIQQLK 58

Query: 751 ENTFKCNDKADTS-SLIINNKLICNETVEVPKDSKSKICSERKRVNENELQQDEPPAK— 807

E + N D ++ K +E + K+KI E + + E + + AK Sb_jct: 59 EELQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKI-KELETILETQKVERSHSAKLE 117

Query: 808 KGSIHVSSAITEDQKKSEEVRPNIAE-IEDIRVLQENNEGLRAFLLTIENELKNEK 862 + + S I + ++ +E + ++ + +++ + L L+ + + N L++ K Sbjct: 118 QDILEKESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKLKEEITQLTNNLQDMKHLLQ 177

Query: 863 —EEKAELNKQIVH-FQQELSLSEKKNLTLSKEVQQIQSNYDIAIAELHVQKSKNQEQEE 919

EE+ E N+Q ++ELΞ S + L ++Q+ + +Y A+L K K + ++ Sb_jct: 178 LKEEEEETNRQETEKLKEELSASSARTQNLKADLQRKEEDY ADL KEKLTDAKK 230

Query: 920 KIMKLSNEIETATRSITNNVSQIKLMHTKIDEL-RTLDSVSQISNIDLLNLRDLSNGSEE 978

+1 ++ E+ S+ + + KL+ KI+EL + + SQ +D+ R + E+ Sbjct: 231 QIKQVQKEV ΞVMRD--EDKLLRIKINELEKKKNQCSQ—ELDMKQ-RTIQQLKEQ 280

Query: 979 DNLPNTQLDLLGNDYLVSKQVKEYRIQEPNRENSFHSSIEAIWEECKEIVKASSKKSHQI 1038

N N +++ Y + K+ ++E E+ ++E + E + K ++ Sbjct: 281 LN—NQKVEEAIQQY—ERACKDLNVKEKIIED-MRMTLEEQEQTQVEQDQVLEAKLEEV 335

Query: 1039 EELEQQIEKLQAEVKGYKDENNRLKEKEHKNQDDLLKEKETLIQQLKEELQEKNVT 1094

E L ++EK + + + +NN+ KEH+N D+L + L +L+E Q+ N Sbjct: 336 ERLATELEKWKEKCNDLETKNNQRΞNKEHENNTDVLGKLTNLQDELQESEQKYNADRKKW 395

Query: 1095 LDVQIQHVVEGKRA LSELTQGVTCYKAKIKELETILETQKVERSHSAKLEQDI 1147

L+ ++ + + K A + + + + + E+E IL Q E+ + ++ Sbjct: 396 LEEKMMLITQAKEAENIRNKEMKKYAEDRERFFKQQNEME-ILTAQLTEKDSDLQKWRE- 453

Query: 1148 LEKESIILKLERNLKEFQEHLQDSVKNTKDLNVKELK-LKEEITQLTNNLQDMKHLLQLK 1206

E++ ++ LE LK + +V+ KD +++LK + E +++ + D+K + Sbjct: 454 -ERDQLVAALEIQLKAL ISSNVQ—KDNEIEQLKRIIΞETSKIETQIMDIK PKR 504

Query: 1207 EEEEETNRQETEKLKEELSASSARTQN 1233

+ ++ +TE L S + ++ Sbjct: 505 ISSADPDKLQTEPLSTSFEISRNKIED 531

Score = 186 (27.9 bits), Expect = 3.2e-10, P = 3.2e-10 Identities = 131/674 (19%), Positives = 294/674 (43%)

Query: 673 LELKFNQIKAELAKTKGELIKT-KEELKKRENESDSLIQELETSNKKIITQNQRIKELIN 731

L+ K ++ + +L K K LI+ KEEL+++ D IQ + + + Q + Sbjct: 35 LKEKEHKNQDDLLKEKETLIQQLKEELQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKA 94

Query: 732 IIDQKEDTINEFQNL-KSHMENTFKCNDKADTSSLIINNKLICNETVEVPKDSKSKICSE 790

I + E TI E Q + +SH + D + S+I+ + E E +DS Sbjct: 95 KIKELE-TILETQKVERSHSAKLEQ—DILEKESIILKLERNLKEFQEHLQDS VKN 147

Query: 791 RKRVNENELQ-QDEPPAKKGSIHVSSAITEDQKKSEEV-RPNIAEI-EDIRVLQENNEGL 847

K +N EL+ ++E ++ + + +++ EE R ++ E++ + L Sbjct: 148 TKDLNVKELKLKEEITQLTNNLQDMKHLLQLKEEEEETNRQETEKLKEELSASSARTQNL 207

Query: 848 RAFLLTIENELKNEKEEKAELNKQIVHFQQELSLSEKKNLTLSKEVQQI QSNYDI 902

+A L E + + KE+ + KQI Q+E+S+ ++ L ++ ++ Q + ++ Sbjct: 208 KADLQRKEEDYADLKEKLTDAKKQIKQVQKEVSVMRDEDKLLRIKINELEKKKNQCSQEL 267

Query: 903 AIAELHVQKSKNQEQEEKIMKLSNEIETATRΞITNNVSQIKLMHTKIDEL-RTLDSVSQI 961

+ + +Q+ K Q +K+ + + E A + + I+ M ++E +T Q+ Sbjct: 268 DMKQRTIQQLKEQLNNQKVEEAIQQYERACKDLNVKEKIIEDMRMTLEEQEQTQVEQDQV 327

Query: 962 SNIDLLNLRDLSNGSEEDNLPNTQLDLLGNDYLVSKQVKEYRI—QEPNRENSFHSSIEA 1019

L + L+ E+ L+ N + + + N ++ S + Sbjct: 328 LEAKLEEVERLATELEKWKEKCNDLETKNNQRSNKEHENNTDVLGKLTNLQDELQESEQK 387

Query: 1020 IWEECKEIVKASSKKSHQIEELEQQIEKLQAEVKGYKDENNRLKEKEHKNQ—DDLLKEK 1077

+ K+ ++ Q +E E K E+K Y ++ R +++++ + L EK Sbjct: 388 YNADRKKWLEEKMMLITQAKEAENIRNK EMKKYAEDRERFFKQQNEMEILTAQLTEK 444

Query: 1078 ETLIQQLKEELQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKVER 1137

++ +Q+ +EE + L++Q++ ++ + + ++ ++ET + K +R

Sbjct: 445 DSDLQKWREERDQLVAALEIQLKALISSNVQKDNEIEQLKRIIΞETSKIETQIMDIKPKR 504

Query: 1138 SHSAKLEQDILEKESIILKLERNLKEFQEHLQDS VKNTKDLNVKELKLKEEITQLT 1193

SA ++ E S ++ RN E + DS +N + + +L+ + T L Sbjct: 505 ISSADPDKLQTEPLSTSFEISRNKIEDGSVVLDSCEVSTENDQSTRFPKPELEIQFTPLQ 564

Query: 1194 NNLQDMKH LLQLKEEEEETNRQETEKLKEEL-SASSARTQNLKADLQRKEEDYADLK 1249

N +KH + + + ++++ +++E+L + + + +L+ D + Sbjct: 565 PNKMAVKHPGCTTPVTVKIPKARKRKSNEMEEDLVKCENKKNATPRTNLKFPISDDRNSS 624

Query: 1250 EKLTDAKKQIKQVQKEVSVMRDEDKLLRIKINELEKKKNQCSQEL-DMKQRTIQQLKEQL 1308

K + K 1+ K+ +R + + I +N KKK Q+ D Q + L+ + Sbjct: 625 VK-KEQKVAIRPSSKKTYSLRSQASI—IGVNLATKKKEGTLQKFGDFLQHSPSILQSKA 681

Query: 1309 NNQKVEEAIQQYERACKDLNVKEKIIEDMR 1338

+K+ E + + + + + KE + + R Sbjct: 682 —KKIIETMSSSKLSNVEAS-KENVSQPKR 708 Score = 165 (24.8 bits), Expect = 5.8e-08, P = 5.8e-08 Identities = 140/626 (22%), Positives = 271/626 (43%)

Query: 536 VEELENAEETQNVETKLLDEDLDKTLEENKAFISHEEKRKLLDLIEDLKKKLINEKKEK- 594

+EELE E E K +D + L+E + H+ + LL E L ++L E +EK Sbjct: 11 IEELEQQIEKLQAEVKGY-KDENNRLKEKE HKNQDDLLKEKETLIQQLKEELQEKN 65

Query: 595 LTLEFKIREEVT QEFTQYWAQREADFKE—TLLQEREILEENAERRLAIFKDLVG 647

+TL+ +1+ V E TQ +A KE T+L+ +++ E + +L +D++ Sbjct: 66 VTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKV-ERSHSAKLE—QDILE 122

Query: 648 KCDT REEAAKDICATKVETEEATACLELKFNQIKAELAKTKGELIKTKEELKKRENE 704

K E K+ ++ + T L +K ++K E+ + L K L+ +E E Sbjct: 123 KESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKLKEEITQLTNNLQDMKHLLQLKEEE 182

Query: 705 SDSLIQELETSNKKIITQNQRIKELINIIDQKEDTINEFQNLKSHMENTFKCNDKADTSS 764

++ QE E +++ + R + L + +KE+ + + + + K K + S Sbjct: 183 EETNRQETEKLKEELSAΞSARTQNLKADLQRKEEDYADLKEKLTDAKKQIKQVQK-EVSV 241

Query: 765 LIINNKLICNETVEVPKDSKSKICSERKRVNENELQQDEPPAKKGSIHVSSAITEDQKKS 824

+ +KL+ + E+ K K CS+ + + +QQ + V Al + ++

Sbjct: 242 MRDEDKLLRIKINELEK—KKNQCSQELDMKQRTIQQLKEQLNNQK—VEEAIQQYERAC 297

Query: 825 EEVRPNIAEIEDIRVLQENNEGLRAFLLTIENELKNEKEEKAELNKQIVHFQQELSLSEK 884

+++ IED+R+ E E + + + L+ + EE L ++ ++++ + E Sbjct: 298 KDLNVKEKIIEDMRMTLEEQEQTQ VEQDQVLEAKLEEVERLATELEKWKEKCNDLET 354

Query: 885 KNLTLSKEVQQIQSNYDIAIAELHVQKSKNQEQEEKIMKLSNE-IETATRSITN N 938

KN S + + ++N D+ + +L + + QE E+K + +E IT N Sbjct: 355 KNNQRSNK—EHENNTDV-LGKLTNLQDELQESEQKYNADRKKWLEEKMMLITQAKEAEN 411

Query: 939 VSQIKLMHTKIDELRTLDSVSQISNIDL-LNLRD—LSNGSEEDNLPNTQLDLLGNDYLV 995

+ ++ D R +++ + L +D L EE + L++ + Sbjct: 412 IRNKEMKKYAEDRERFFKQQNEMEILTAQLTEKDSDLQKWREERDQLVAALEIQLKALIS 471

Query: 996 SKQVKEYRIQEPNRENSFHSSIEA-IWE-ECKEIVKASSKKSHQIEELEQQIEKLQAEVK 1053

S K+ I++ R Ξ Ξ IE I + + K I A K Q E L E + +++ Sbjct: 472 SNVQKDNEIEQLKRIISETSKIETQIMDIKPKRISSADPDKL-QTEPLΞTSFEISRNKIE 530

Query: 1054 GYKDENNRLKEKEHKNQDDLLKEKE TLIQQLKEELQEKNVTLDVQIQHVVEGKRA 1108

+ + +Q + E T +Q K ++ T V ++ KR Sbjct: 531 DGSVVLDSCEVSTENDQSTRFPKPELEIQFTPLQPNKMAVKHPGCTTPVTVKIPKARKRK 590

Query: 1109 LSELTQG-VTCYKAKIKELETILETQ-KVERSHSAKLEQDILEKES 1152

+E+ + V C K T L+ +R+ S K EQ + + S Sbjct: 591 ΞNEMEEDLVKCENKKNATPRTNLKFPISDDRNΞSVKKEQKVAIRPS 636

Score = 143 (21.5 bits), Expect = 1.3e-05, P = 1.3e-05 Identities = 164/684 (23%), Positives = 304/684 (44%)

Query: 295 QKRKMLR-LSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGIKHQSVAFTKLNNASΞ 349

+K +++ L ++++ + D+Q V + K A L G+ +L Sbjct: 49 EKETLIQQLKEELQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKV 108

Query: 350 -RSHSI-FTVKILQIEDSEMSRVIRVSELSLCDLAGSERTMKTQNEGE-RLRETGNINTS 406

RSHS IL+ E + + E L S + K N E +L+E T+ Sbjct: 109 ERSHSAKLEQDILEKESIILKLERNLKEFQE-HLQDSVKNTKDLNVKELKLKEEITQLTN 167

Query: 407 LLTLGKCINVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCYLAYDET 466

L K + LK E+ +Q + +L+ N K + + Y E Sbjct: 168 NLQDMKHLLQLKEEEEETNRQETEKLKEELSASSARTQNLKADL QRKEEDYADLKEK 224

Query: 467 LNVLKFSAIAQKVCVPDTLNSSQDKLFGPVKSSQDVΞLDSNSNSKILNVKRATISWENSL 526

L K I Q V ++ +DKL +K ++ + N S+ L++K+ TI Sbjct: 225 LTDAK-KQIKQ-VQKEVSVMRDEDKLLR-IKINE-LEKKKNQCSQELDMKQRTIQQLKEQ 280

Query: 527 EDLMEDEDLVEELENAEETQNVETKLLDEDLDKTLEENKAFISHEEKRKLLDL-IEDLKK 585

+ + E+ +++ E A + NV+ K++ ED+ TLEE + + E+ ++L+ +E++++ Sbjct: 281 LNNQKVEEAIQQYERACKDLNVKEKII-EDMRMTLEEQEQ—TQVEQDQVLEAKLEEVER 337

Query: 586 KLIN-EK-KEKLT-LEFKIREEVTQEFTQYWAQREADFKETLLQEREILEE NAERR 638

EK KEK LE K + +E + K T LQ+ E+ E NA+R+ Sbjct: 338 LATELEKWKEKCNDLETKNNQRSNKEHEN NTDVLGKLTNLQD-ELQEΞEQKYNADRK 393

Query: 639 LAIFKDLVGKCDTREEAAKDICATKVETEEATACLELKFNQIKAELAKTKGELIKTKEEL 698

+ + ++ T+ + A++I K E ++ E F Q + E+ +L + +L Sbjct: 394 KWLEEKMM—LITQAKEAENI-RNK-EMKKYAEDRERFFKQ-QNEMEILTAQLTEKDSDL 448

Query: 699 KKRENESDSLIQELETSNKKIITQN-QR IKELINIIDQKEDTINEFQNLKSHMENTF 754

+K E D L+ LE K +1+ N Q+ I++L II + + ++K ++ Sbjct: 449 QKWREERDQLVAALEIQLKALISSNVQKDNEIEQLKRIISETSKIETQIMDIKPKRISSA 508

Query: 755 KCNDKADTSSLIINNKLICN—ETVEVPKDSKSKICSERK RVNENELQ-QDEP—PA 806

DK T L + ++ N E V DS ++ +E R + EL+ Q P P

Sbjct: 509 D-PDKLQTEPLSTSFEISRNKIEDGSVVLDS-CEVSTENDQSTRFPKPELEIQFTPLQPN 566

Query: 807 KKGSIH—VSSAITEDQKKSEEVRPNIAEIEDIRVLQENNEGLRA FLLTIENELKNE 861

K H ++ +T K+ + + N E + ++ + N R F ++ + +

Sbjct: 567 KMAVKHPGCTTPVTVKIPKARKRKSNEMEEDLVKCENKKNATPRTNLKFPISDDRNSSVK 626

Query: 862 KEEKAEL NKQIVHFQQELSLSEKKNLTLSKEVQQIQSNYDIAIAELHVQKSKNQEQE 918

KE+K + +K+ + + S+ NL K+ +Q D + +SK ++

Sbjct: 627 KEQKVAIRPSSKKTYSLRSQASIIGV-NLATKKKEGTLQKFGDFLQHSPSILQSKAKKII 685

Query: 919 EKIM—KLSNEIETATRSITNNVSQIKLMHTKI—DELRT-LDSVSQISNID 965 E + KLSN +E + NVSQ K K+ E+ + +D Q+ +D

Sbjct: 686 ETMSSSKLSN-VEASKE NVSQPKRAKRKLYTSEISΞPIDISGQVILMD 732

Score = 133 (20.0 bits), Expect = 1.6e-04, P = 1.6e-04

Identi .ties == 94/426 (22%), Positives = 188/426 (44%)

Query: 527 EDLM-EDEDLVEELENAEETQNVETKLLDEDLDKTLEENKAFISHEEKRKLLDL-IEDLK 584 +DL+ E E L+++L+ + +NV LD + +E +A + I++L+

Sbjct: 44 DDLLKEKETLIQQLKEELQEKNVT LDVQIQHVVEGKRALSELTQGVTCYKAKIKELE 100

Query: 585 KKLINEKKEKLTLEFKIREEVTQ-EFTQYWAQREA-DFKETLLQEREILEENAERRLAIF 642

L +K E+ + K+ +++ + E +R +F+E L + ++ + L +

Sbjct: 101 TILETQKVER-SHSAKLEQDILEKESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKL- 158

Query: 643 KDLVGKCDTREEAAKDICATKVETEEATACLELKFNQIKAELAKTKGELIKTKEELKKRE 702 K+ + + + K + K E EE + ++K EL+ + K +L+++E

Sbjct: 159 KEEITQLTNNLQDMKHLLQLKEEEEETN RQETEKLKEELSASSARTQNLKADLQRKE 215

Query: 703 NESDSLIQELETSNKKIITQNQRIKELINIIDQK-EDTINEFQNLKSHMENTFKCNDKA- 760

+ L ++L T KK I Q Q+ ++ D+ INE + K+ +

Sbjct: 216 EDYADLKEKL-TDAKKQIKQVQKEVSVMRDEDKLLRIKINELEKKKNQCSQELDMKQRTI 274

Query: 761 DTSSLIINNKLICNETVE VPKDΞ—KSKICSE-RKRVNENE LQQDEPPAKKGS 810

+NN+ + E ++ KD K KI + R + E E ++QD+ K

Sbjct: 275 QQLKEQLNNQKV-EEAIQQYERACKDLNVKEKIIEDMRMTLEEQEQTQVEQDQVLEAKLE 333

Query: 811 IHVSSAITEDQKKSEEVRP-NIAEIEDIRVLQENNEGLRAFLLTIENELKNEKEEKAELN 869

V TE +K E+ + ENN + L +++EL+ E E+K +

Sbjct: 334 -EVERLATELEKWKEKCNDLETKNNQRΞNKEHENNTDVLGKLTNLQDELQ-ESEQKYNAD 391

Query: 870 KQIVHFQQELSLSEKKNLTLSKEVQQIQSNYDIAIAELHVQKSKNQEQEEKIMKLSNEIE 929 ++ ++++ L +T +KE + I++ + K E E+ K NE+E

Sbjct: 392 RK-KWLEEKMML ITQAKEAENIRNK EMKKYAEDRERFFKQQNEME 435

Query: 930 TATRSITNNVSQIKLMHTKIDEL 952

T +T S ++ + D+L

Sbjct: 436 ILTAQLTEKDSDLQKWREERDQL 458

Pedant information for DKFZphtes3_35b4, frame 3

Report for DKFZphtes3_35b4.3

[LENGTH] 1780

[MW] 206176.77

[pi] 5.60

[HOMOL] TREMBL :U93121_1 product: "M-phase phosphoproteιn-1"; Human M-phase phosphoproteιn-1 mRNA, partial eds. 0.0

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YEL061c] 2e-37

[FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YEL061C] 2e-37

[FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YELOδlc] 2e-37

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YELOδlc] 2e-37

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL058w]

7e-30

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDL058w] 7e-30

[FUNCAT] 30.05 organization of centrosome [S. cerevisiae, YPR141c] 3e-23

[FUNCAT] 11.01 stress response [S. cerevisiae, YPR141c] 3e-23

[FUNCAT] 03.07 pheromone response, matmg-type determination, sex-specific proteins

[S. cerevisiae, YPR141c] 3e-23

[FUNCAT] 03.13 meiosis [S. cerevisiae, YPR141c] 3e-23

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YPR141c] 3e-23

[FUNCAT] 09.10 nuclear biogenesis [S. cerevisiae, YPR141C] 3e-23

[FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YKR095w] le-21 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YLR309c] 6e-20

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YHR023w

MYOl - myosιn-1 isoform] 4e-19

[FUNCAT] 03.25 cytokinesis [S. cerevisiae, YHR023w MYOl - myosιn-1 isoform] 4e-19

[FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YNL250w] le-15

[FUNCAT] 1 genome replication, transcription, recombination and repair [M. jannaschii MJ1322] 2e-14

[FUNCAT] 30.13 organization of chromosome structure [Ξ. cerevisiae, YDR285w] 2e-09

[FUNCAT] 09.04 biogenesis of cytoskeleton [S. cerevisiae, YKL179c] 3e-09

[FUNCAT] 09.13 biogenesis of chromosome structure [S. cerevisiae, YLR086w] 2e-07

[FUNCAT] 03.01 cell growth [S. cerevisiae, YNL079c] 2e-07

[FUNCAT] 08.99 other intracellular-transport activities [S. cerevisiae, YNL079c]

2e-07

[FUNCAT] 03.22.01 cell cycle check point proteins [S. cerevisiae, YGL086w] le-06

[FUNCAT] 10.05.99 other pheromone response activities [S. cerevisiae, YHR158c]

3e-06

[FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YDR217c] 4e-06

[FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YJR134c] 2e-05

[FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae,

YAL035W] 2e-04

[FUNCAT] r general function prediction [M. jannaschii, MJ1254] 0.001

[BLOCKS] BL00387A

[BLOCKS] BL00411H

[BLOCKS] BL00411G

[BLOCKS] BL00411F

[BLOCKS] BL00411E Kinesin motor domain proteins

[BLOCKS] BL00411D Kinesin motor domain proteins

[BLOCKS] BL00411C Kinesin motor domain proteins

[BLOCKS] BL00411B Kinesm motor domain proteins

[BLOCKS] BL00411A Kmesin motor domain proteins

[SCOP] d2kιn.l 3.29.1.5.3 Kmesin [Rat (Rattus norvegicus) 2e-68

[SCOP] d2tmab_ 1.105.4.1.1 Tropomyosin [rabbit (Oryetolagus cumeulus) 4e-05

[SCOP] d3kar 3.29.1.5.4 Kinesin [Baker's yeast (Saccharomyce 2e-09

[EC] 3.6.1.32 Myosin ATPase 5e-25

[PIRKW] nucleus 4e-27

[PIRKW] phosphotransferase 3e-16

[PIRKW] duplication 6e-20

[PIRKW] c trulline 6e-18

[PIRKW] tandem repeat 4e-24

[PIRKW] heterodimer 3e-28

[PIRKW] endocytosis le-23

[PIRKW] heart le-17

[PIRKW] transmembrane protein 2e-28

[PIRKW] serme/threo ne-specific protein kinase 3e-16

[PIRKW] zmc fmger le-23

[PIRKW] surface antigen 2e-16

[PIRKW] DNA binding le-25

[PIRKW] metal binding le-23

[PIRKW] muscle contraction 4e-24

[PIRKW] heterotetramer 4e-24

[PIRKW] acetylated amino end 2e-19

[PIRKW] actin binding 5e-25

[PIRKW] mitosis 3e-58

[PIRKW] microtubule binding 3e-58

[PIRKW] ATP 3e-58

[PIRKW] thick filament 4e-24

[PIRKW] phosphoprotein 9e-29

[PIRKW] leucine zipper le-12

[PIRKW] skeletal muscle 8e-24

[PIRKW] disulfide bond le-12

[PIRKW] heterotrimer le-29

[PIRKW] calcium binding 6e-18

[PIRKW] alternative splicing 4e-21

[PIRKW] P-loop 2e-63

[PIRKW] coiled coil 3e-58

[PIRKW] heptad repeat le-25

[PIRKW] methylated ammo acid 4e-24

[PIRKW] peripheral membrane protein le-23

[PIRKW] dimer le-12

[PIRKW] cardiac muscle le-17

[PIRKW] hydrolase 5e-25

[PIRKW] microtubule 6e-15

[PIRKW] muscle 7e-23

[PIRKW] membrane protein 6e-20

[PIRKW] GTP binding 8e-22

[PIRKW] EF hand 6e-18

[PIRKW] cell division le-25

[PIRKW] cytoskeleton 4e-24

[PIRKW] hair 6e-18

[PIRKW] Golgi apparatus 8e-24

[PIRKW] calmodulin binding le-₂3 [SUPFAM] unassigned Ser/Thr or Tyr-specific protein kinases 3e-16

[SUPFAM] myosin motor domain homology 5e-25

[SUPFAM] alpha-actinin actin-bmding domain homology le-13

[SUPFAM] kinesin-related protein KIP1 9e-27

[SUPFAM] kmesin-related protein CIN8 4e-36

[SUPFAM] kinesin heavy chain 4e-24

[SUPFAM] plectm le-13

[SUPFAM] trichohyalm 6e-18

[SUPFAM] kinesm-related protein KIF3 le-29

[SUPFAM] kinesm-related protein KIF2 3e-20

[SUPFAM] ribosomal protein S10 homology le-13

[SUPFAM] giantin 8e-24

[SUPFAM] protein kinase homology 3e-16

[SUPFAM] protein kinase C zinc-bmding repeat homology 2e-13

[SUPFAM] kinesin-related protein unc-104 8e-26

[SUPFAM] human early endosome antigen 1 le-23

[SUPFAM] unassigned kmesin-related proteins le-28

[SUPFAM] Mycoplasma ge talium hypothetical protein MG218 4e-17

[SUPFAM] myosin heavy chain 5e-25

[SUPFAM] conserved hypothetical P115 protein 4e-20

[SUPFAM] centromere protein E 5e-24

[SUPFAM] calmodulin repeat homology 6e-18

[SUPFAM] kinesin-related protein KLP61F le-25

[SUPFAM] hypothetical protein MJ0914 3e-12

[SUPFAM] kinesin-related protein MKLP-1 2e-63

[SUPFAM] pleckstrm repeat homology 8e-26

[SUPFAM] hypothetical protein MJ1322 4e-13

[SUPFAM] kinesm-related protein KIF1B 3e-28

[SUPFAM] kinesin motor domain homology 2e-63

[SUPFAM] k esin-related protein KLPA 7e-25

[SUPFAM] kinesin-related protein nodA le-12

[SUPFAM] kinesin-related protein Eg5 5e-30

[PROSITE] ATP_GTP_A 1

[PFAM] Kinesin motor domain

[KW] Irregular

[KW] 3D

[KW] LOW_COMPLEXITY 7.53 %

[KW] COILED_COIL 19.78 %

SEQ MESNFNQEGVPRPSYVFSADPIARPSEINFDGIKLDLSHEFSLVAPNTEANSFESKDYLQ SEG COILS 3kar-

SEQ VCLRIRPFTQSEKELESEGCVHILDSQTVVLKEPQCILGRLSEKSSGQMAQKFSFSKVFG SEG COILS 3kar-

SEQ PATTQKEFFQGCIMQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENIGILPRTLNVLF SEG COILS 3kar-

SEQ DSLQERLYTKMNLKPHRSREYLRLSSEQEKEEIASKSALLRQIKEVTVHNDSDDTLYGSL SEG COILS 3kar-

SEQ TNSLNISEFEESIKDYEQANLNMANSIKFSVWVSFFEIYNEYIYDLFVPVSSKFQKRKML SEG COILS 3kar- EEEEEEEEEEETTEEEETTTCC CCEE

SEQ RLSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGIKHQSVAFTKLNNASSRSHSIFTVKIL SEG COILS 3kar- EEETTTTE-EEEETTCCEEECCGGGHHHHHHHHHHHHCCTTTTCHHHHHHCEEEEEEEEE

SEQ QIEDΞEMSRVIRVSELSLCDLAGSERTMKTQNEGERLRETGNINTSLLTLGKCINVLKNS SEG COILS 3kar- E—EETTTTCEEEEEEEEEECCCCCCC CCCHHHHHHHHHHHHHHHHHHHHHHHHTT

SEQ EKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCYLAYDETLNVLKFSAIAQKVC SEG COILS 3kar- TTTT—TCCTTTTTHHHHHHGGGCTTTTEEEEEEEECCCGGGHHHHHHHHHHHH

SEQ VPDTLNSSQDKLFGPVKΞSQDVSLDSNSNSKILNVKRATISWENSLEDLMEDEDLVEELE SEG xxxxxxxxxxxxxxxxxx

COILS

3kar-

SEQ NAEETQNVETKLLDEDLDKTLEENKAFISHEEKRKLLDLIEDLKKKLINEKKEKLTLEFK

SEG xxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxx ..

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

3kar-

SEQ IREEVTQEFTQYWAQREADFKETLLQEREILEENAERRLAIFKDLVGKCDTREEAAKDIC

SEG

COILS CCCCCCC

3kar-

SEQ ATKVETEEATACLELKFNQIKAELAKTKGELIKTKEELKKRENESDSLIQELETSNKKII

SEG

COILS ccccccccccccccc

3kar-

SEQ TQNQRIKELINIIDQKEDTINEFQNLKSHMENTFKCNDKADTSSLIINNKLICNETVEVP

SEG

COILS CCCCCCCCCCCCCCC

3kar-

SEQ KDSKSKICSERKRVNENELQQDEPPAKKGSIHVSSAITEDQKKSEEVRPNIAEIEDIRVL

SEG

COILS CCCC

3kar-

SEQ QENNEGLRAFLLTIENELKNEKEEKAELNKQIVHFQQELSLSEKKNLTLSKEVQQIQSNY

SEG xxxxxxxxxxxxxxxx

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

3kar-

SEQ DIAIAELHVQKSKNQEQEEKIMKLSNEIETATRSITNNVSQIKLMHTKIDELRTLDSVΞQ

SEG

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

3kar-

SEQ ISNIDLLNLRDLSNGSEEDNLPNTQLDLLGNDYLVSKQVKEYRIQEPNRENSFHSSIEAI

SEG

COILS

3kar-

SEQ WEECKEIVKASSKKSHQIEELEQQIEKLQAEVKGYKDENNRLKEKEHKNQDDLLKEKETL

SEG xxxxxxxxxxxxx

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

3kar-

SEQ IQQLKEELQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKVERSHS

SEG

COILS ccccccccccccccccccccccccccccccccc . . . .

3kar-

SEQ AKLEQDILEKESIILKLERNLKEFQEHLQDΞVKNTKDLNVKELKLKEEITQLTNNLQDMK

SEG

COILS cccccccccccccccccccccccccccccc ccccccccccccccccccccccccc

3kar-

SEQ HLLQLKEEEEETNRQETEKLKEELSASΞARTQNLKADLQRKEEDYADLKEKLTDAKKQIK

SEG . xxxxxxxxxxxxxxxxxxx

COILS ccccc cccccccccccccccccccccccccccccccc

3kar-

SEQ QVQKEVSVMRDEDKLLRIKINELEKKKNQCSQELDMKQRTIQQLKEQLNNQKVEEAIQQY

SEG

COILS cccccccccccc

3kar-

SEQ ERACKDLNVKEKIIEDMRMTLEEQEQTQVEQDQVLEAKLEEVERLATELEKWKEKCNDLE

SEG xxxxxxxxxxxxxxxxx

COILS CCCCCCCCCCCCCCCCCCCCCCCCCC

3kar-

SEQ TKNNQRSNKEHENNTDVLGKLTNLQDELQESEQKYNADRKKWLEEKMMLITQAKEAENIR

SEG

COILS CC

3kar-

SEQ NKEMKKYAEDRERFFKQQNEMEILTAQLTEKDΞDLQKWREERDQLVAALEIQLKALISSN

SEG COILS

3kar-

SEQ VQKDNEIEQLKRIISETSKIETQIMDIKPKRISSADPDKLQTEPLSTSFEISRNKIEDGS

SEG

COILS

3kar-

SEQ VVLDSCEVSTENDQSTRFPKPELEIQFTPLQPNKMAVKHPGCTTPVTVEIPKARKRKSNE

SEG

COILS

3kar-

SEQ MEEDLVKCENKKNATPRTNLKFPISDDRNSSVKKEQKVAIRPSSKKTYSLRSQAΞIIGVN

SEG

COILS

3kar-

SEQ LATKKKEGTLQKFGDFLQHSPSILQSKAKKIIETMSΞSKLSNVEASKENVSQPKRAKRKL

SEG

COILS

3kar-

SEQ YTSEISSPIDISGQVILMDQKMKESDHQIIKRRLRTKTAK

SEG

COILS

3kar-

Prosite for DKFZphtes3_35b4.3

PS00017 152->160 ATP GTP A PDOC00017

Pfam for DKFZphtes3_35b4.3

HMM_NAME Kinesin motor domain

HMM *RCRPlNeREιndgcscvVQWPpWtGyktvhnghegds phks

R+RP+ + E++ + +V + ++++ ++ + ++

Query 64 RIRPFTQSEKELESEGCVHILDSQTVVLKEPQCILGRLSEKSSGQMAQK 112

HMM FtFDHVFWWncTQedVYdtvAHPIVDDcFhGYNCTIFAYGQTGSGKTYTM F+F +VF++++TQ++ +++ + V+D+++G IF+YG T SGKTYT

Query 113 FSFSKVFGPATTQKEFFQGCIMQPVKDLLKGQSRLIFTYGLTNSGKTYTF 162

HMM MGpggehPDHmGIIPRcCHDIFdrldkfqekDhdFW

G +++GI+PR+++ +FD++ + +++

Query 163 QG TEENIGILPRTLNVLFDSLQERL-YTKMNLKPHRSREYLRLSSE 207

HMM

Query 208 QEKEEIASKSALLRQIKEVTVHNDSDDTLYGSLTNSLNISEFEESIKDYE 257

HMM hVkCSYMEIYNEelYDLLCPnP... qhMkpLnlHEHPN

+V +S++EIYNE+IYDL +P++ Q++K L++ + +

Query 258 QANLNMANSIKFSVWVSFFEIYNEYIYDLFVPVSSKFQKRKMLRLSQDVK 307

HMM MGpYVqGCTEfHVcSYeDachWIWqGnknRHVAaTnMNdhSSRSHtlFTI ++++++ V +A +++ +G K+ VA T++N SSRSH+IFT+

Query 308 GYSFIKDLQWIQVSDSKEAYRLLKLGIKHQSVAFTKLNNASSRSHSIFTV 357

HMM HVeQrHk . qcdehvcHSKMNLVDLAGSERvnrTGAEGQRIKEGcNINqSL ++ Q + + +++S ++L DLAGΞER+ +T+ EG RL+E +NIN SL

Query 358 KILQIEDSEMSRVIRVSELSLCDLAGSERTMKTQNEGERLRETGNINTSL 407

HMM ttLGnVInaLaDgqTKYmYgghgHIPYRDSKLTWlLQDSLGGNcKTcMIA +TLG++IN+L + + + +H+P+R+SKLT+ +Q + G +K CMI+

Query 408 LTLGKCINVLKNSE KSKFQQHVPFRESKLTHYFQSFFNGKGKICMIV 454

HMM CIWPadWNYEETLSTLRYAdRAKnlkNkPQINEDPca* +1+ + Y+ETL++L++ + A+++ + ++N+++++

Query 455 NISQCYLAYDETLNVLKFSAIAQKVCVPDTLNSSQDK 491 DKFZphtes3_35b5

group: metabolism

DKFZphtes3_35b5 encodes a novel 466 ammo acid protein, with similarity to bovme accessory subunit for vacuolar ATPase and rat C7-1 protein.

The vacuolar proton-ATPase (V-ATPase) translocates protons into intracellular organelles or across the plasma membrane of specialized cells. The catalytic domain consists of a hexamer of 3 A subunits and 3 B subunits, plus accessory subunits C, D, and E. The rat homolog C7-1 seems to be enriched in aged adult rats in the frontal cortex.

The novel protein can find application in modulating the v-ATPase activity in endocytic and secretory organelles. strong similarity to bovine vacuolar ATPase (EC 3.6.1.-) chain A complete cDNA, complete eds potential start at Bp 8 , EST hits matches perfect to 154197 hypothetical protein, but posess 186 aa additional at N-termmus

Sequenced by DKFZ

Locus : unknown

Insert length: 2043 bp

Poly A stretch at pos. 2033, polyadenylation signal at pos. 2012

1 GGCGGCCATG GCGACGGCTC GAGTGCGGAT GGGGCCGCGG TGCGCCCAGG 51 CGCTCTGGCG CATGCCGTGG CTGCCGGTGT TTTTGTCGTT GGCGGCGGCG

101 GCGGCGGCGG CAGCGGCGGA GCAGCAGGTC CCGCTGGTGC TGTGGTCGAG

151 TGACCGGGAC TTGTGGGCTC CTGCGGCCGA CACTCATGAA GGCCACATCA

201 CCAGCGACTT GCAGCTCTCT ACCTACTTAG ATCCCGCCCT GGAGCTGGGT

251 CCCAGGAATG TGCTGCTGTT CCTGCAGGAC AAGCTGAGCA TTGAGGATTT

301 CACAGCATAT GGCGGTGTGT TTGGAAACAA GCAGGACAGC GCCTTTTCTA

351 ACCTAGAGAA TGCCCTGGAC CTGGCCCCCT CCTCACTGGT GCTTCCTGCC

401 GTCGACTGGT ATGCAGTCAG CACTCTGACC ACTTACCTGC AGGAGAAGCT

451 CGGGGCCAGC CCCTTGCATG TGGACCTGGC CACCCTGCGG GAGCTGAAGC

501 TCAATGCCAG CCTCCCTGCT CTGCTGCTCA TTCGCCTGCC CTACACAGCC

551 AGCTCTGGTC TGATGGCACC CAGGGAAGTC CTCACAGGCA ACGATGAGGT

601 CATCGGGCAG GTCCTGAGCA CACTCAAGTC CGAAGATGTC CCATACACAG

651 CGGCCCTCAC AGCGGTCCGC CCTTCCAGGG TGGCCCGTGA TGTAGCCGTG

701 GTGGCCGGAG GGCTAGGTCG CCAGCTGCTA CAAAAACAGC CAGTATCACC

751 TGTGATCCAT CCTCCTGTGA GTTACAATGA CACCGCTCCC CGGATCCTGT

801 TCTGGGCCCA AAACTTCTCT GTGGCGTACA AGGACCAGTG GGAGGACCTG

851 ACTCCCCTCA CCTTTGGGGT GCAGGAACTC AACCTGACTG GCTCCTTCTG

901 GAATGACTCC TTTGCCAGGC TCTCACTGAC CTATGAACGA CTCTTTGGTA

951 CCACAGTGAC ATTCAAGTTC ATTCTGGCCA ACCGCCTCTA CCCAGTGTCT 1001 GCCCGGCACT GGTTTACCAT GGAGCGCCTC GAAGTCCACA GCAATGGCTC 1051 CGTCGCCTAC TTCAATGCTT CCCAGGTCAC AGGGCCCAGC ATCTACTCCT 1101 TCCACTGCGA GTATGTCAGC AGCCTGAGCA AGAAGGGTAG TCTCCTCGTG 1151 GCCCGCACGC AGCCCTCTCC CTGGCAGATG ATGCTTCAGG ACTTCCAGAT 1201 CCAGGCTTTC AACGTAATGG GGGAGCAGTT CTCCTACGCC AGCGACTGTG 1251 CCAGCTTCTT CTCCCCCGGC ATCTGGATGG GGCTGCTCAC CTCCCTGTTC 1301 ATGCTCTTCA TCTTCACCTA TGGCCTGCAC ATGATCCTCA GCCTCAAGAC 1351 CATGGATCGC TTTGATGACC ACAAGGGCCC CACTATTTCT TTGACCCAGA 1401 TTGTGTGACC CTGTGCCAGT GGGGGGGTTG AGGGTGGGAC GGTGTCCGTG 1451 TTGTTGCTTT CCCACCCTGC AGCGCACTGG ACTGAAGAGC TTCCCTCTTC 1501 CTACTGCAGC ATGAACTGCA AGCTCCCCTC AGCCCATCTT GCTCCCTCTT 1551 CAGCCCGCTG AGGAGCTTTC TTGGGCTGCC CCCATCTCTC CCAACAAGGT 1601 GTACATATTC TGCGTAGATG CTAGACCAAC CAGCTTCCCA GGGTTCGTCG 1651 CTGTGAGGCG TAAGGGACAT GAATTCTAGG GTCTCCTTTC TCCTTATTTA 1701 TTCTTGTGGC TACATCATCC CTGGCTGTGG ATAGTGCTTT TGTGTAGCAA 1751 ATGCTCCCTC CTTAAGGTTA TAGGGCTCCC TGAGTTTGGG AGTGTGGAAG 1801 TACTACTTAA CTGTCTGTCC TGCTTGGCTG CCGTTATCGT TTTCTGGTGA 1851 TGTTGTGCTA ACAATAAGAA GTACACGGGT TTATTTCTGT GGCCTGAGAA 1901 GGAAGGGACC TCCACGACAG GTGGGCTGGG TGCGATCGCC GGCTGTTTGG 1951 CATGTTCCCA CCGGGAGTGC CGGGCAGGAG CATGGGGTGC TTGGTTGTTT 2001 CCTTCCTAAT AAAATAAACG CGGGTCGCCA TGCAAAAAAA AAA

BLAST Results

No BLAST result Medline entries

95014142:

A novel accessory subunit for vacuolar H(+) -ATPase from chromaffm granules .

97215246:

Identification of a rat brain gene associated with aging by

PCR differential display method.

Peptide information for frame 2

ORF from 8 bp to 1405 bp; peptide length: 466 Category: strong similarity to known protein

1 MATARVRMGP RCAQALWRMP WLPVFLSLAA AAAAAAAEQQ VPLVLWSSDR

51 DLWAPAADTH EGHITSDLQL STYLDPALEL GPRNVLLFLQ DKLSIEDFTA

101 YGGVFGNKQD SAFSNLENAL DLAPSSLVLP AVDWYAVSTL TTYLQEKLGA

151 SPLHVDLATL RELKLNASLP ALLLIRLPYT ASSGLMAPRE VLTGNDEVIG

201 QVLSTLKSED VPYTAALTAV RPSRVARDVA VVAGGLGRQL LQKQPVSPVI

251 HPPVSYNDTA PRILFWAQNF SVAYKDQWED LTPLTFGVQE LNLTGSFWND

301 SFARLSLTYE RLFGTTVTFK FILANRLYPV SARHWFTMER LEVHSNGSVA

351 YFNASQVTGP SIYSFHCEYV SSLSKKGSLL VARTQPSPWQ MMLQDFQIQA

401 FNVMGEQFSY ASDCASFFSP GIWMGLLTSL FMLFIFTYGL HMILSLKTMD

451 RFDDHKGPTI SLTQIV

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_35b5, frame 2

TREMBL:AF035387_1 gene: "C7-1"; product: "C7-1 protein"; Rattus norvegicus C7-1 protein (C7-1) mRNA, complete eds., N = 1, Score = 2088, P = 3.8e-216

PIR:A55116 vacuolar ATPase (EC 3.6.1.-) chain Ac45 - bovme, N = 1, Score = 2011, P = 5.5e-208

PIR:I54197 hypothetical protein - human, N = 1, Score = 1464, P = 5.1e-150

>TREMBL:AF035387_1 gene: "C7-1"; product: "C7-1 protein"; Rattus norvegicus C7-1 protein (C7-1) mRNA, complete eds. Length = 463

HSPs:

Score = 2088 (313.3 bits), Expect = 3.8e-216, P = 3.8e-216 Identities = 408/463 (88%), Positives = 426/463 (92%)

Query: 4 ARVRMGPRCAQALWRMPWLPVFLSLAAAAAAAAAEQQVPLVLWSSDRDLWAPAADTHEGH 63

+R+R G R A LW + LSL A AAA AAEQQVPLVLWSSDRDLWAP ADTHEGH Sbjct: 8 SRIRTGTRWAPVLW LLLSLVAVAAAVAAEQQVPLVLWSSDRDLWAPVADTHEGH 61

Query: 64 ITSDLQLSTYLDPALELGPRNVLLFLQDKLSIEDFTAYGGVFGNKQDSAFSNLENALDLA 123

ITSD+QLSTYLDPALELGPRNVLLFLQDKLSIEDFTAYGGVFGNKQDSAFSNLENALDLA Sbjct: 62 ITSDMQLSTYLDPALELGPRNVLLFLQDKLΞIEDFTAYGGVFGNKQDSAFSNLENALDLA 121

Query: 124 PSSLVLPAVDWYAVSTLTTYLQEKLGASPLHVDLATLRELKLNASLPALLLIRLPYTASS 183

PSSLVLPAVDWYA+STLTTYLQEKLGASPLHVDLATL+ELKLNASLPALLLIRLPYTASS Sbjct: 122 PSSLVLPAVDWYAISTLTTYLQEKLGASPLHVDLATLKELKLNASLPALLLIRLPYTASS 181

Query: 184 GLMAPREVLTGNDEVIGQVLSTLKSEDVPYTAALTAVRPΞRVARDVAVVAGGLGRQLLQK 243

GLMAPREVLTGNDEVIGQVLSTL+SEDVPYTAALTAVRPSRVARDVA+VAGGLGRQLLQ Sbjct: 182 GLMAPREVLTGNDEVIGQVLSTLESEDVPYTAALTAVRPSRVARDVAMVAGGLGRQLLQT 241

Query: 244 QPVSPVIHPPVSYNDTAPRILFWAQNFSVAYKDQWEDLTPLTFGVQELNLTGSFWNDSFA 303

Q SP IHPPVΞYNDTAPRILFWAQNFSVAYKD+W+DLT LTFGV+ LNLTGSFWNDSFA Sbjct: 242 QVASPAIHPPVSYNDTAPRILFWAQNFSVAYKDEWKDLTSLTFGVENLNLTGSFWNDSFA 301

Query: 304 RLSLTYERLFGTTVTFKFILANRLYPVSARHWFTMERLEVHSNGSVAYFNASQVTGPSIY 363 LSLTYE LFG TVTFKFILA+R YPVSAR+WFTMERLE+HSNGSVA+FN ΞQVTGPSIY Sbjct: 302 MLSLTYEPLFGATVTFKFILASRFYPVSARYWFTMERLEIHSNGSVAHFNVSQVTGPSIY 361

Query: 364 SFHCEYVSSLSKKGSLLVARTQPSPWQMMLQDFQIQAFNVMGEQFSYASDCASFFSPGIW 423

SFHCEYVSSLSKKGSLLV PS WQM L +FQIQAFNV GEQFSYASDCA FFSPGIW Sbjct: 362 SFHCEYVSSLSKKGSLLVTNV-PSLWQMTLHNFQIQAFNVTGEQFΞYASDCAGFFSPGIW 420

Query: 424 MGLLTSLFMLFIFTYGLHMILSLKTMDRFDDHKGPTISLTQIV 466

MGLLT+LFMLFIFTYGLHMILSLKTMDRFDD KGPTI+LTQIV Sbjct: 421 MGLLTTLFMLFIFTYGLHMILSLKTMDRFDDRKGPTITLTQIV 463

Pedant information for DKFZphtes3_35b5, frame 2

Report for DKFZphtes3_35b5.2

[LENGTH] 466

[MW] 51621.44 [pi] 5.73

[HOMOL] TREMBL:AF035387 1 gene: "C7-1 product: "C7-1 protein"; Rattus norvegicus C7-1 protein (C7-1) mRNA, complete eds ;. 0.0

[PIRKW] hydrolase 0.0

[PROSITE] MYRISTYL 7

[PROSITE] CAMP PHOSPHO SITE 1

[PROSITE] CK2 PHOSPHO SITE 7

[PROSITE] TYR PHOSPHO SITE 1

[PROSITE] PKC PHOSPHO SITE 8

[PROSITE] ASN GLYCOSYLATION 7

[KW] SIGNAL PEPTIDE 38

[KW] TRANSMEMBRANE 1

[KW] LOW COMPLEXITY 11.59 %

SEQ MATARVRMGPRCAQALWRMPWLPVFLSLAAAAAAAAAEQQVPLVLWSSDRDLWAPAADTH

SEG xxxxxxxxx

PRD ccceeeecccchhhhhhhcccchhhhhhhhhhhhhhhhhccceeeecccccccccccccc

MEM

SEQ EGHITSDLQLSTYLDPALELGPRNVLLFLQDKLSIEDFTAYGGVFGNKQDSAFSNLENAL

SEG

PRD ccccccchhhhhccccccccccccceeecccccccccccccccccccccchhhhhhhhcc

MEM

SEQ DLAPSSLVLPAVDWYAVSTLTTYLQEKLGASPLHVDLATLRELKLNASLPALLLIRLPYT

SEG xxxxxxxxxxxxxxx ...

PRD ccccccccccccceeeeehhhhhhhhhhccccchhhhhhhhhhhhhhcchhhhhhhcccc

MEM

SEQ ASSGLMAPREVLTGNDEVIGQVLSTLKSEDVPYTAALTAVRPSRVARDVAVVAGGLGRQL

SEG xxxxxxxxxxxxxxxxxxxx ..

PRD cccccceeeeeecccccchhhhhhhccccccchhhhhhhccccceeehhhhhccccchhh

MEM

SEQ LQKQPVSPVIHPPVSYNDTAPRILFWAQNFSVAYKDQWEDLTPLTFGVQELNLTGSFWND

SEG

PRD hhhhccccccccccccccccceeeeeccccceeeeccccccccceeeeeecccccccccc

MEM

SEQ SFARLSLTYERLFGTTVTFKFILANRLYPVSARHWFTMERLEVHSNGSVAYFNASQVTGP

SEG

PRD hhhhhhhhhhhhccceeeeeeecccccccccchhhhhhhhhhcccccceeeeeecccccc

MEM

SEQ SIYSFHCEYVSSLSKKGSLLVARTQPSPWQMMLQDFQIQAFNVMGEQFSYASDCASFFSP

SEG xxxxxxxxxx

PRD ceeeeeeeeeeecccccceeeeeccccchhhhhhhhheeeeccccccccccccccccccc

MEM MMMMMM

SEQ GIWMGLLTSLFMLFIFTYGLHMILΞLKTMDRFDDHKGPTISLTQIV

SEG

PRD ccchhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccceeeeccc

MEM MMMMMMMMMMMMMMMMMMMMMMM

Prosite for DKFZphtes3_35b5.2

PS00001 166->170 ASN_GLYCOSYLATION PDOC00001 PS00001 257->261 AΞN_GLYCOSYLATION PDOC00001 PS00001 269->273 ASN GLYCOSYLATION PDOC00001 SO

o o CO

H U α.

SO

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DKFZphtes3_35e21

group: differentiation/development

DKFZphtes3_35e21.2 encodes a novel 104 ammo acid putative interleukin precursor, related to mterleukm-7.

Due to the close relationship to human ιnterleukιn-7, the novel interleukin is expected to act as a new growth factor for human B lineage cells. Additionally, the protein should induce the gene rearrangement of the T-cell receptor repertoire, leading to thymocyte commitment, and subsequently induce both cytotoxic T-cell- and lymphocyte-activated killer cells.

This new interleukin could find clinical application in a variety of conditions of hematoly phopoietic failure and different tumours, because of its recruitment of B cell lineage cells, cytotoxic T-cell- and lymphocyte-activated killer cells. similarity to mterleukιn-7 precursor complete cDNA, complete eds, EST hits

Sequenced by DKFZ

Locus: unknown

Insert length: 2095 bp

Poly A stretch at pos. 2085, polyadenylation signal at pos. 2067

1 GGATGAAAGT GATTTAATTC ATTTTTAGAA TTTTTTTTTT GTTTTGTTTT

51 AGCAACATGC TGAACAACTA ATTTACTTTA AAAATAAGCC AGTTAAAACA

101 AAGGACGCTA AGCCCAAGTG GGGGGCAATA TTAGTCAGGA TCTTTGGGGT

151 CTAATTCCAG ACCAACTTTC AGAAGCACTT CTTTGTCTCT GTTCTCACCT

201 CTGCTGTCCC TCTCTTCCCT CATCCCCTAA GAGAGACAAA GATAAAAGCC

251 CACCTGCATC CCTAAGTCTT ACTGAGATCA GCCACCCCAG GGGAGAGAAA

301 CTGGATCTAC TTACAGCCAC CCCCTGTTTC CATCCATATA CTTACTTCCC

351 CCAATTTGCA TGTGATTATG GAAACAAGTC ATGCTCATGA AAGCAACTGT

401 AAAATAAAAG GTTATGGAGT AGTTCAGCAA CTTCTTCACA GCCAGCTTTG

451 TGGAGCTGGG GAGGACTTAG GGCCCATTGG AGTCTCTTAT GTGTACAGCT

501 TCAGGGCTGT CCCTTTCAGT TTGATTTTAA GCAATGCCTC ACTTCATAGC

551 TTAGGGGGTA AGGATTCCAT TCAGGTAGGT TGTCTAAAGG AACTAATGGG

601 ACCTCTCAGT GAATTAGCTG ACCAGATTTT AGGAAATCTT TTTAATTTCT

651 ATGATTTTCC TTCTCACATT TTGAAATGGT AAAATTGACT GGAAATAATT

701 TTTCTTGGTG CCTTATTGGT TTTCCTTGCA AACCTTTCTC ATATTTTCTC

751 ATGACCATTG CCAGTGACCA AGGCCCATGT GTGTGTTGTG TGTAATTGTG

801 GGCATGTACA AGCTTAAATA ACGTGCCGAC AGCACTGTTT CAAAGTTGGT

851 ATTCATTAGG CTGTTGCCTC CTGGGCTGGA GCTGCGCTAA TCCTGACACC

901 GGCTGCCAGG AGAAAACCTC ATGGATCACA CACCAAACCT TAATAACAGC

951 ATCCGTGACC TGCACTCTCC AGTACAGAAT GGGAACCCCA GAGCTAGGAA

1001 ATGTAGTTGT ATATTTTAAT GAACTGCTAC CCCAGCCAAA GAAGCTTCTT

1051 TCACTTTTGT GCTCTACAGA AAGCCCAAGG GGGGTAGGAG GGACAGAGCT

1101 TTGAATAACT GCTTTCTAAC ACTAAATGTG GCCAACAGGA CAGAGCACAT

1151 CACACGTATA GGCAGGTGTG AGGGACAGTG GCTAAGAATT GCCTGCTCCC

1201 TCTGCATGCT CTTTCTTGTT TCCAAAGTCC AATCAAGTGA TCCTGGGAAA

1251 CAAATCTGTC TGGATTGCGG AGGGTGGTTC TGAAAGAACT GCCAAGACGT

1301 TAAAGAAGGG TGAAGAGTAG GCAGAATATA AGTAGCTAAC CTGAGTCAAG

1351 ACTCTCAAAA GCTAGCAGCC TGATGACAAT AGGATTTATT TCAGCCAGGA

1401 TAGTGTCTGT CTGTGAGTGC ATCATTTTAA GACAGTATGA CTTCATGTTG

1451 TTACAAACTA TGTATAGTAT GTATGTTTTG TGGGTTGTAT ATATACATAA

1501 TATATATTAT ATATATATAT GAGAGATTTG GTGACTTTTG ATACGGGTTT

1551 GGTGCAGGTG AATTTATTAC TGAGCCAAAT GAGGCACATA CCGAGTCAGT

1601 AGTTGAAGTC CAGGGCATTC GATACTGTTT ATGATTTCCA TATATGTATA

1651 GTGCCTATCC CATGCTGTAG TCACTGTTAT GTTAAATCCA GAAGTTACAC

1701 TAGAGCCAGC GATACTTTAT TTGTAGACAA TCAATTTGAA TCCATATGTT

1751 ATTACTGGCA GATGATACAT GATTACAGTT CTGAATCTGT AACACTTACA

1801 AAAGGAAACC CAGAGCAGCT TGATGAGTTT TTGTTTCTGC TTCGTTCCTG

1851 GGAGTCAGTA GAAACAGCAG TTGTATGTGG TTATGTTAGT CTCAAGATAC

1901 TTAATTTGTT GACCTTACTT CAGAAAAATT TTGTATGTAT TATATTTGTG

1951 GGAAGGTAAA ATAATCATTT GAGATTTTTA TCAAATATGA AGATTAGTTA

2001 TTTATGAAAA ACAAAGAAAT GTCTATTTTT CTTTGTTCCC AATTAATGTA

2051 GATAAATTTT AAAATGCATT AAAGTAATGG TCCGGAAAAA AAAAA

BLAST Results

No BLAST result Medlme entries

89098903:

Human interleukin 7: molecular cloning and growth factor activity on human and murine B-lmeage cells.

Peptide information for frame 2

ORF from 368 bp to 679 bp; peptide length: 104 Category: similarity to known protein

1 METSHAHESN CKIKGYGVVQ QLLHSQLCGA GEDLGPIGVS YVYSFRAVPF 51 SLILSNAΞLH ΞLGGKDSIQV GCLKELMGPL SELADQILGN LFNFYDFPSH 101 ILKW

BLASTP hits

Entry B32223 from database PIR: ιnterleukm-7 precursor (clone 1) - human

Score = 66, P = 7.0e-01, identities = 21/70, positives = 33/70

Alert BLASTP hits for DKFZphtes3_35e21, frame 2

PIR:B32223 mterleukm-7 precursor (clone 1) - human, N = 1, Score = 66, P = 0.72

TREMBL :PADAL1_1 gene: "dall"; P.abies dall mRNA, N = 2, Score = 59, P = 0.77

PIR:C32223 ιnterleukιn-7 precursor (clone 4) - human, N = 1, Score = 66, P = 0.79

TREMBL :PRU76726_1 gene: "PrMADS3"; product: "MADS-box protein"; Pinus radiata MADS-box protein (PrMADS3) mRNA, complete eds., N = 2, Score = 59, P = 0.94

>PIR:B32223 ιnterleukιn-7 precursor (clone 1) - human Length = 133

HSPs:

Score = 66 (9.9 bits), Expect = 1.3e+00, P = 7.2e-01 Identities = 21/68 (30%), Positives = 33/68 (48%)

Query: 39 VSYVYSFRAVPFSLIL SNASLHSLGGK—DSIQVGCLKELMGPLSELADQILGNL 91

VS+ Y F P L+L S+ + GK +S+ + + +L+ + E+ L N Sbjct: 4 VSFRYIFGLPPLILVLLPVASSDCDIEGKDGKQYESVLMVSIDQLLDSMKEIGΞNCLNNE 63

Query: 92 FNFYDFPSHI 101

FNF F HI Sbjct: 64 FNF--FKRHI 71

Pedant information for DKFZphtes3_35e21, frame 2

Report for DKFZphtes3_35e21.2

[LENGTH] 104

[MW] 11339.12

[pi] 5.87

[PROSITE] MYRISTYL 2

[PROSITE] PKC PHOSPHO SITE 1

[PROSITE] ASN_GLYCOSYLATION 1

[KW] Alpha_Beta

SEQ METSHAHESNCKIKGYGVVQQLLHSQLCGAGEDLGPIGVSYVYSFRAVPFSLILSNASLH PRD ccchhhhhcccccccchhhhhhhhhhhcccccccccceeeeeeeccccceeeeecccccc SEQ SLGGKDSIQVGCLKELMGPLSELADQILGNLFNFYDFPSHILKW PRD cccccceeeccccccccccchhhhhhhhcccccccccccccccc

Prosite for DKFZphtes3_35e21.2

PS00001 56- ->60 ASN GLYCOSYLATION PDOC00001

PS00005 44- ->47 PKC PHOSPHO_SITE PDOC00005

PS00008 63- ->69 MYRISTYL PDOC00008

PS00008 89- ->95 MYRISTYL PDOC00008

(No Pfam data available for DKFZphtes3_35e21.2)

DKFZphtes3_35g6 group: testes derived

DKFZphtes3_35g6 encodes a novel 482 amino acid protein with high partial similarity to H. sapiens chromosome 19, cosmid R27216.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes .

strong similarity to R27216_l complete cDNA, complete eds, EST hits

Sequenced by DKFZ

Locus: /map="15"

Insert length: 3177 bp

Poly A stretch at pos. 3167, polyadenylation signal at pos. 3148

1 GGAGGCAGCG CCGGCCTCCG GAGGCGGCCT GGGCGATGGC GGCGGAGTTT

51 TGTCCATAAC CTGGGCAACC GCGCAGCTGG AGGATGGCCT CACTCGGGCC

101 TGCCGCAGCT GGGGAGCAGG CGTCGGGGGC TGAGGCGGAG CCGGGCCCCG

151 CGGGGCCGCC GCCGCCGCCC TCACCGTCCT CTCTGGGGCC CCTGCTCCCC

201 CTGCAGCGGG AACCTCTCTA CAACTGGCAG GCGACCAAGG CGTCGCTGAA

251 GGAGCGCTTC GCCTTCCTCT TCAACTCGGA GCTGCTGAGC GATGTGCGCT

301 TCGTACTGGG CAAGGGTCGC GGCGCCGCCG CCGCTGGGGG CCCGCAGCGC

351 ATCCCCGCCC ACCGCTTCGT GCTGGCGGCC GGCAGCGCCG TCTTTGACGC

401 CATGTTCAAC GGCGGCATGG CCACCACGTC GGCCGAGATC GAGCTGCCGG

451 ACGTGGAGCC CGCAGCCTTC CTGGCGCTGC TGAGATTTCT ATATTCAGAT

501 GAAGTTCAAA TTGGTCCAGA AACAGTTATG ACCACTCTTT ATACTGCCAA

551 GAAATACGCA GTCCCAGCCT TGGAAGCACA CTGTGTAGAA TTTCTCACCA

601 AACATCTTAG GGCAGATAAT GCCTTTATGT TACTTACTCA GGCTCGATTA

651 TTTGATGAAC CTCAGCTTGC TAGTCTTTGT CTAGATACAA TAGACAAAAG

701 CACAATGGAT GCAATAAGTG CAGAAGGGTT TACTGATATT GATATAGATA

751 CACTCTGTGC AGTTTTAGAG AGAGACACAC TCAGTATTCG AGAAAGTCGA

801 CTTTTTGGAG CTGTTGTACG CTGGGCAGAA GCAGAATGTC AGAGACAACA

851 ATTACCTGTG ACTTTTGGGA ATAAACAAAA AGTTCTAGGA AAAGCACTTT

901 CCTTAATCCG GTTCCCACTG ATGACAATTG AGGAATTTGC AGCAGGTCCT

951 GCTCAATCTG GAATTTTGTC AGATCGTGAA GTGGTAAACC TCTTTCTTCA

1001 TTTTACTGTC AACCCTAAAC CCCGAGTTGA ATACATTGAC CGACCAAGAT

1051 GCTGTCTCAG GGGAAAGGAA TGCTGCATCA ATAGATTCCA GCAAGTAGAA

1101 AGCCGCTGGG GTTACAGTGG GACGAGTGAT CGAATCAGAT TCACAGTTAA

1151 TAGAAGGATC TCTATAGTTG GATTTGGCTT GTATGGATCT ATTCATGGCC

1201 CTACAGATTA TCAAGTGAAT ATACAGATCA TTGAATATGA GAAAAAGCAA

1251 ACCCTGGGAC AGAATGATAC CGGCTTTAGT TGTGATGGGA CAGCTAACAC

1301 ATTCAGGGTC ATGTTCAAGG AACCCATAGA GATCCTGCCC AATGTGTGCT

1351 ACACAGCATG TGCAACACTC AAAGGTCCAG ATTCCCACTA TGGCACAAAA

1401 GGATTGAAGA AAGTAGTGCA TGAGACACCT GCTGCAAGCA AGACTGTTTT

1451 TTTCTTTTTT AGTTCCCCTG GCAATAATAA TGGCACTTCA ATAGAAGATG

1501 GACAAATTCC AGAAATCATA TTTTATACAT AATTTAGCAT TATAATACAT

1551 CTTGGCTAAA TAATACCATA CAATCTAGTG TCAAAAACAT AAATGGCCAC

1601 AAAAAAGTAG TTTGAGTGTT ATGAATATTT AAAATTGTAA GATAAGAAAC

1651 AGTTTCTTAG AGCAGATAGA AAAATGCTTA TTTAAATCTT TGCATGATTT

1701 AAAAACAGAT TTTCCATTTT CTTACAACTT TAAGAGAAAA GAACTGGGTT

1751 TAATGGTTTA AAAAAAAGCA CAGCTTTTTC ACCTTCATCT TGTATAATTT

1801 CATAGATTGG CTGACTTAGG GTCTTTCAAT AGTTTGGGAA TTGAAAGATT

1851 CTTGTTATAT ATAGCTAGTT TGGGTTTGTT TTTGTTTTAA CTATTTTGAA

1901 GGTTAGGTGA GATGGGCAAA TAGGCTTAAC TATTTTGAAG GTTGGATGAA

1951 AAGAGATGGG TCAGTATTCC TACAGAATTC TTATTAACTC AAATAACTAA

2001 ATTTCAGAAA ATTAAGAAGC TGACTTTATA TTTGGTGGTT TGAAGTATCT

2051 TGTTGTTAGC ATTTGTAATA ATGCTAAAAA AGGCCTAATA AAATGCCCAA

2101 GAAAATATTC AGTGCATTTA TAGAGAAGGA TATTTTGTAG TAGTATAGTA

2151 ATGTGTTATG TAGTACAGTT TTAAAGCTAT AAATGGAATT TTGTGTAAAT

2201 TCACAAAAAT GTGATATAAA CAGGATCTAA GACTGGATTC CCTGTCACTA

2251 AACTGCACCA CTATACCTGT CTCTCTGTGT GGGGGACACT GCTGATGATT

2301 CCCAAGATTG AGATGATGAC GGTGATGACG ACTGGGTGAA CAGCCATCAC

2351 TTCAACATTG TGATAATCCT TCACAGCAAG AAACCGAATA AAATACTAAC

2401 ATTTCTAACA ACTGCTCTGA CATTGTAAAG AGATCCAACA GAATCACTCC

2451 TGCTGAAAAA TACGCTTTCT GCCACCTACA CATTTCTATT TAGGAAGTAA

2501 AATTTGCTTC ATGGTCATGA CCCCATTAGT CAGTGTTACA GCTGTGTTGG

2551 GGATAGGAAG TATATCTGGC AGATTGACAT TTATACACTT TTTTATAAAG

2601 CAGATTTTAA AATATAGTAA CATCCATTTT TTTCCCTTGA AAGTGATTCT

2651 CTTATAAAAA ATGAAAGTGG AGTTTAAGGT ATATCAAATC GTTGTGGAAG

2701 GTGATTAAAA ATCAAAATTC TTTTAAATAT CAACTTAATT TTTTCTAAGT 2751 AAGATACAAA AAATTTTCAT CTAAAGTAAT ATTTCACTTT ATATTGTAAA 2801 GAAGGTAGGT ATATTGGTGG CTGAGGTCTC TTGAAATTGC TAAAGGGAAA 2851 TTTTTCTATG GTAATGCTCT TACGGATATA AGCCTCAGTT AAATGGAATT 2901 ATCTATGGGA TGTGTGGTTC TGGTTAACTA AAAATTAACC AGTAAACACT 2951 CTGTAGTAAC CATTACAGAA AATACTTCTG CCTTAAAAAA TATGATATGC 3001 CAGAGATGAG TTAGTGTTTC TTGACGTTGG AGACCTATAA ATGCCTCATC 3051 TGTTGTACTG AACAATTGAA ACTGCATGCA GCCATAAAAG GGACAAGAAA 3101 CAGAACTGTT TACTAACTTT GGGACATCCC CTGGAGTTTT TAAAAATAAA 3151 TAAATATATA TATATATAAA AAAAAAA

BLAST Results

Entry G37753 from database EMBL: SHGC-63477 Human Homo sapiens STS genomic. Score = 1627, P = 3.0e-66, identities = 327/329

Entry G37752 from database EMBL: SHGC-63476 Human Homo sapiens STS genomic. Score = 1578, P = 6.2e-64, identities = 320/324

Medlme entries

No Medline entry

Peptide information for frame 3

ORF from 84 bp to 1529 bp; peptide length: 482 Category: similarity to unknown protein

1 MASLGPAAAG EQASGAEAEP GPAGPPPPPΞ PSSLGPLLPL QREPLYNWQA

51 TKASLKERFA FLFNSELLSD VRFVLGKGRG AAAAGGPQRI PAHRFVLAAG

101 SAVFDAMFNG GMATTSAEIE LPDVEPAAFL ALLRFLYSDE VQIGPETVMT

151 TLYTAKKYAV PALEAHCVEF LTKHLRADNA FMLLTQARLF DEPQLASLCL

201 DTIDKSTMDA ISAEGFTDID IDTLCAVLER DTLSIRESRL FGAVVRWAEA

251 ECQRQQLPVT FGNKQKVLGK ALSLIRFPLM TIEEFAAGPA QSGILΞDREV

301 VNLFLHFTVN PKPRVEYIDR PRCCLRGKEC CINRFQQVES RWGYSGTSDR

351 IRFTVNRRIS IVGFGLYGSI HGPTDYQVNI QIIEYEKKQT LGQNDTGFSC

401 DGTANTFRVM FKEPIEILPN VCYTACATLK GPDSHYGTKG LKKVVHETPA

451 ASKTVFFFFS SPGNNNGTSI EDGQIPEIIF YT

BLASTP hits

Entry AC005306_2 from database TREMBL: product: "R27216_l"; Homo sapiens chromosome 19, cosmid R27₂16, complete sequence.

Score = 1₂98, P = 1.9e-13₂, identities = 245/297, positives = 268/297

Entry CEF38H4_9 from database TREMBLNEW: gene: "F38H4.7"; Caenorhabditis elegans cosmid F38H4

Score = 1237, P = 5.6e-126, identities = 248/446, positives = 322/446

Entry AC004678_1 from database TREMBL: product: "R34094_l"; Homo sapiens chromosome 19, cosmid R34094, complete sequence.

Score = 555, P = 1.0e-53, identities = 112/137, positives = 123/137

Alert BLASTP hits for DKFZphtes3_35g6, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphtes3_35g6, frame 3

Report for DKFZphtes3_35g6.3

[LENGTH] 482

[MW] 52771.47

[pi] 5.79 [HOMOL] TREMBL:AC005306_2 product: "R27216_l"; Homo sapiens chromosome 19, cosmid R27216, complete sequence, le-142

[BLOCKS] BL01075D Acetate and butyrate kinases family proteins

[SUPFAM] POZ domain homology 3e-08

[SUPFAM] A55R protein middle region homology 5e-06

[SUPFAM] A55R protein 5e-06

[SUPFAM] A55R protein carboxyl-terminal homology 5e-06

[PROSITE] MYRISTYL

[PROSITE] CAMP_PHOSPHO_SITE

[PROSITE] CK2_PHOSPHO_SITE

[PROSITE] TYR_PHOSPHO_SITE

[PROSITE] PKC_PHOSPHO_SITE

[PROSITE] ASN_GLYCOSYLATION

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 11.20 %

SEQ MASLGPAAAGEQASGAEAEPGPAGPPPPPSPΞSLGPLLPLQREPLYNWQATKASLKERFA SEG ....xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccccchhhhhhhhcccccccccccccccccccccccccccccchhhhhhhhhhhhhh

SEQ FLFNSELLSDVRFVLGKGRGAAAAGGPQRIPAHRFVLAAGSAVFDAMFNGGMATTSAEIE SEG xxxxxxxxxxx PRD hhhccccccceeeeecccccccccccccchhhhheeecccchhhhhhhhcchhhhhhhee

SEQ LPDVEPAAFLALLRFLYSDEVQIGPETVMTTLYTAKKYAVPALEAHCVEFLTKHLRADNA SEG PRD ecccchhhhhhhhhhhhccceeechhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccch

SEQ FMLLTQARLFDEPQLASLCLDTIDKSTMDAIΞAEGFTDIDIDTLCAVLERDTLSIRESRL SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhccccchhhhhh

SEQ FGAVVRWAEAECQRQQLPVTFGNKQKVLGKALSLIRFPLMTIEEFAAGPAQSGILSDREV SEG

PRD hhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhcceeecccccccccccccchhhhh

SEQ VNLFLHFTVNPKPRVEYIDRPRCCLRGKECCINRFQQVESRWGYSGTSDRIRFTVNRRIS SEG PRD hhhhheeeccccceeeeecccceeeccceeehhhhhhhhhccccccccccchhhhhceee

SEQ IVGFGLYGSIHGPTDYQVNIQIIEYEKKQTLGQNDTGFSCDGTANTFRVMFKEPIEILPN SEG PRD eeeccccccccccchhhhhhhcchhhhhhhhccccccccccccccceeeeeccceeeccc

SEQ VCYTACATLKGPDSHYGTKGLKKVVHETPAASKTVFFFFSSPGNNNGTSIEDGQIPEIIF SEG xxxxxx

PRD ccceeeeecccccccccccceeeeeeeccccceeeeeeeecccccccccccccccceeec

SEQ YT SEG PRD

Prosite for DKFZphtes3_35g6.3

PS00001 394->398 ASN_GLYCOSYLATION PDOC00001 PS00001 466->470 ASN_GLYCOSYLATION PDOC00001 PS00004 357->361 CAMP_PHOSPHO_SITE PDOC00004 PS00004 387->391 CAMP_PHOSPHO_SITE PDOC00004 PS00005 54->57 PKC_PHOSPHO_SITE PDOC00005 PS00005 154-M57 PKC_PHOΞPHO_SITE PDOC00005 PS00005 234->237 PKC_PHOSPHO_SITE PDOC00005 PS00005 296->299 PKC_PHOSPHO_SITE PDOC00005 PS00005 348->351 PKC_PHOSPHO_SITE PDOC00005 PS00005 406->409 PKC_PHOSPHO_SITE PDOC00005 PS00005 428->431 PKC_PHOSPHO_SITE PDOC00005 PS00006 14->18 CK2_PHOSPHO_SITE PDOC00006 PS00006 54->58 CK2_PHOSPHO_SITE PDOC00006 PS00006 115->119 CK2_PHOSPHO_SITE PDOC00006 PS00006 206->210 CK2_PHOSPHO_SITE PDOC00006 PS00006 217->221 CK2_PHOSPHO_SITE PDOC00006 PS00006 234->238 CK2_PHOSPHO_SITE PDOC00006 PS00006 281->285 CK2_PHOSPHO_SITE PDOC00006 PS00006 296->300 CK2_PHOSPHO_SITE PDOC00006 PS00006 468->472 CK2_PHOSPHO_SITE PDOC00006 PS00007 430->437 TYR_PHOSPHO_SITE PDOC00007 PS00008 80->86 MYRISTYL PDOC00008 PS00008 110->116 MYRISTYL PDOC00008 PS00008 365->371 MYRISTYL PDOC00008 PS00008 392->398 MYRISTYL PDOC00008

PS00008 402->408 MYRISTYL PDOC00008

PS00008 463->469 MYRISTYL PDOC00008

(No Pfam data available for DKFZphtes3_35g6.3)

DKFZphtes3_35kl6

group: metabolism

DKFZphtes3_35kl6 encodes a novel 666 ammo acid protein with weak similarity to fatty acid-CoA synthetaseses/ligases .

The novel protein contains a putative AMP-binding domain signature, which is present in enzymes, which act via an ATP-dependent covalent binding of AMP to their substrate. This domain is found in several CoA synthetases, such as acetate-CoA ligase (EC 6.2.1.1), long- chain-fatty-acid-CoA ligase (EC 6.2.1.3), bile acid-CoA ligase. Therefore it is a new fatty acid-CoA synthetasese/ligase with unknown substrate.

The new protein can find application in modulation of fatty acid metabolism and as a new enzyme for biotechnologic production processes. similarity to acyl-CoA synthetase complete cDNA, complete eds, potential start codon at Bp 50, few EST hits, seems to be a testis specific cDNA, 5 of 6 EST hits are from testis deπeved librarys

Sequenced by DKFZ

Locus : unknown

Insert length: 2520 bp

Poly A stretch at pos. 2510, polyadenylation signal at pos. 2490

1 CAGATGTCCC AGCTCCAGTG CTGTGGAGCA TGGTTTCTGC ACACCTGGAA 51 TGACTGGAAC CCCAAAGACT CAAGAAGGAG CTAAAGATCT TGAAGTAGAC

101 ATGAATAAAA CAGAAGTTAC TCCCAGGCTG TGGACCACCT GTCGAGATGG

151 AGAAGTCCTT CTGAGGCTAT CCAAACACGG ACCAGGCCAT GAGACCCCGA

201 TGACCATCCC TGAATTTTTT CGAGAGTCAG TCAACCGATT TGGAACTTAT

251 CCAGCCCTCG CATCCAAGAA TGGCAAAAAG TGGGAAATTC TGAATTTCAA

301 CCAGTACTAT GAGGCTTGTC GGAAGGCTGC AAAATCCTTG ATCAAGCTGG

351 GTTTGGAGCG TTTCCACGGA GTTGGTATCC TGGGGTTTAA CTCTGCAGAG

401 TGGTTTATCA CTGCTGTTGG TGCCATCCTA GCCGGGGGTC TTTGTGTTGG

451 TATTTATGCC ACCAACTCTG CCGAGGCTTG TCAATATGTC ATCACTCATG

501 CCAAAGTGAA CATCTTGCTG GTTGAGAATG ATCAACAGTT ACAGAAAATC

551 CTTTCGATTC CACAGAGCAG CCTAGAGCCC CTAAAAGCGA TCATCCAGTA

601 CAGACTGCCA ATGAAGAAGA ACAACAACTT GTACTCTTGG GATGATTTCA

651 TGGAACTTGG CAGAAGTATC CCTGACACCC AACTGGAGCA GGTCATCGAG

701 AGCCAGAAGG CGAATCAATG CGCAGTGCTC ATCTACACTT CAGGGACCAC

751 AGGCATACCC AAGGGAGTGA TGCTCAGTCA TGACAACATC ACGTGGATTG

801 CAGGAGCAGT GACAAAGGAC TTTAAACTGA CAGACAAGCA TGAGACGGTG

851 GTTAGCTACC TCCCACTCAG CCATATTGCA GCACAGATGA TGGACATCTG

901 GGTACCCATA AAGATTGGGG CGCTCACATA CTTTGCTCAA GCAGATGCTC

951 TCAAGGGCAC CTTGGTAAGT ACTCTAAAGG AGGTAAAACC TACTGTCTTC 1001 ATTGGAGTGC CTCAAATTTG GGAGAAGATA CATGAGATGG TGAAGAAAAA 1051 TAGTGCCAAG TCCATGGGCT TGAAGAAGAA GGCATTCGTG TGGGCAAGAA 1101 ACATTGGCTT CAAGGTCAAC TCAAAAAAGA TGTTGGGGAA ATATAATACT 1151 CCCGTGAGCT ACCGCATGGC TAAGACTCTC GTGTTCAGCA AAGTCAAGAC 1201 ATCCCTTGGC TTGGATCACT GTCACTCTTT TATCAGTGGG ACTGCGCCCC 1251 TCAACCAAGA GACTGCCGAG TTCTTTCTAA GCTTGGACAT ACCTATAGGC 1301 GAGTTGTATG GGTTGAGTGA GAGCTCGGGA CCCCACACGA TATCCAACCA 1351 GAATAACTAC AGGCTTCTAA GCTGTGGCAA GATCTTGACT GGGTGTAAGA 1401 ATATGCTGTT CCAGCAGAAC AAGGATGGCA TTGGGGAGAT CTGCCTCTGG 1451 GGTAGGCACA TCTTCATGGG CTATCTGGAA AGTGAGACTG AAACTACAGA 1501 GGCCATCGAT GATGAAGGCT GGCTACACTC TGGGGATCTG GGCCAGCTGG 1551 ACGGTCTGGG TTTCCTCTAT GTCACCGGCC ACATCAAAGA AATCCTTATC 1601 ACTGCTGGTG GTGAAAATGT GCCCCCCATT CCTGTTGAGA CCTTGGTTAA 1651 GAAGAAGATC CCCATCATCA GTAACGCCAT GTTAGTAGGA GATAAACTGA 1701 AGTTTCTGAG CATGTTGCTG ACGCTGAAGT GTGAGATGAA TCAGATGAGC 1751 GGAGAACCTC TGGACAAGCT GAACTTCGAG GCCATCAACT TCTGTCGGGG 1801 TCTGGGCAGC CAGGCATCCA CCGTGACTGA GATGGTGAAG CAGCAAGACC 1851 CCCTGGTCTA CAAGGCCATC CAGCAAGGCA TCAATGCTGT GAACCAGGAA 1901 GCCATGAACA ATGCACAGAG GATTGAAAAG TGGGTCATCT TGGAGAAGGA 1951 CTTTTCCATC TATGGTGGAG AGCTAGGTCC AATGATGAAA CTTAAGAGAC 2001 ATTTTGTAGC CCAGAAATAC AAAAAACAAA TTGATCACAT GTACCACTGA 2051 CTGCTTTGAT GGAGCTGCTC TCAGCTGTTC TGATGCCTTC AGCAGGAAGA 2101 CCTCATTGCA ATAAGTGAAA TGCTGCTCTA GGTAGAAGCT CTCCCTGCTG 2151 TTTTTAAGAA GCCACATTCC TCATTGGTCA GTTTCTTGAT TGTTCGTCTG 2201 TTGGAGAGGT GCTCCCTAGA AGAACCTGCC ATACGTTTCA AAGCAATAAA 2251 ATCACTGTAT ATCTTTCTAA GGACCTTCAA GTCATGACTC CAGGGAAGCC 2301 TATTGGGAAG TCTACTAAAA ACTGCCTGAT TTACAAGAAA GACCTGAACT 2351 TGTGGGCTCC CATTTGATTT TTTTCTCCTC AGGGGACTCA GACATTAGAA

2401 AGAAAAAGCC TCACAGATTT GAAGAACTGG ACCCCCAAAT CAACTCACCT

2451 GCCTGGAAGC AACTGGGAAA CCCTTCCAAT AAGTCCTGAT AATAAAGCAC

2501 TTCAGGGTCC AAAAAAAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 50 bp to 2047 bp; peptide length: 666 Category: similarity to known protein

1 MTGTPKTQEG AKDLEVDMNK TEVTPRLWTT CRDGEVLLRL ΞKHGPGHETP

51 MTIPEFFRES VNRFGTYPAL ASKNGKKWEI LNFNQYYEAC RKAAKSLIKL

101 GLERFHGVGI LGFNSAEWFI TAVGAILAGG LCVGIYATNS AEACQYVITH

151 AKVNILLVEN DQQLQKILSI PQSSLEPLKA IIQYRLPMKK NNNLYSWDDF

201 MELGRSIPDT QLEQVIESQK ANQCAVLIYT SGTTGIPKGV MLSHDNITWI

251 AGAVTKDFKL TDKHETVVSY LPLSHIAAQM MDIWVPIKIG ALTYFAQADA

301 LKGTLVSTLK EVKPTVFIGV PQIWEKIHEM VKKNSAKSMG LKKKAFVWAR

351 NIGFKVNSKK MLGKYNTPVS YRMAKTLVFS KVKTSLGLDH CHSFISGTAP

401 LNQETAEFFL SLDIPIGELY GLSESSGPHT ISNQNNYRLL SCGKILTGCK

451 NMLFQQNKDG IGEICLWGRH IFMGYLESET ETTEAIDDEG WLHSGDLGQL

501 DGLGFLYVTG HIKEILITAG GENVPPIPVE TLVKKKIPII SNAMLVGDKL

551 KFLSMLLTLK CEMNQMSGEP LDKLNFEAIN FCRGLGSQAS TVTEMVKQQD

601 PLVYKAIQQG INAVNQEAMN NAQRIEKWVI LEKDFSIYGG ELGPMMKLKR

651 HFVAQKYKKQ IDHMYH

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_35kl6, frame 2

TREMBL :AB014531_1 gene: "KIAA0631"; product: "KIAA0631 protein"; Homo sapiens mRNA for KIAA0631 protein, partial eds., N = 1, Score = 1641, P = 8.9e-169

PIR:E70937 probable fadD15 - Mycobacterium tuberculosis (strain H37RV) , N = 2, Score = 532, P = 3.6e-62

PIR:H64041 long-chain-fatty-acid—CoA ligase homolog - Haemophilus influenzae (strain Rd KW20) , N = 2, Score = 486, P = 6.5e-59

>TREMBL:AB014531_1 gene: "KIAA0631"; product: "KIAA0631 protein"; Homo sapiens mRNA for KIAA0631 protein, partial eds. Length = 634

HSPs:

Score = 1641 (246.2 bits), Expect = 8.9e-169, P = 8.9e-169 Identities = 319/628 (50%), Positives = 440/628 (70%)

Query: 38 LRLSKHGPGHETPMTIPEFFRESVNRFGTYPALASKNGKKWEILNFNQYYEACRKAAKSL 97

LR+ P + P T+ F E+++++G AL K KWE ++++QYY R+AAK Sbjct: 2 LRIDPSCP—QLPYTVHRMFYEALDKYGDLIALGFKRQDKWEHISYSQYYLLARRAAKGF 59

Query: 98 IKLGLERFHGVGILGFNSAEWFITAVGAILAGGLCVGIYATNSAEACQYVITHAKVNILL 157

+KLGL++ H V ILGFNS EWF +AVG + AGG+ GIY T+S EACQY+ N+++ Sbjct: 60 LKLGLKQAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYIAYDCCANVIM 119

Query: 158 VENDQQLQKILSIPQSSLEPLKAIIQYRLPM-KKNNNLYSWDDFMELGRSIPDTQLEQVI 216

V+ +QL+KIL I L LKA++ Y+ P K N+Y+ ++FMELG +P+ L+ +1 Sbjct: 120 VDTQKQLEKILKI-WKQLPHLKAVVIYKEPPPNKMANVYTMEEFMELGNEVPEEALDAII 178

Query: 217 ESQKANQCAVLIYTSGTTGIPKGVMLSHDNITWIA—GAVTKDFKLTD-KHETVVSYLPL 273 ++Q+ NQC VL+YTSGTTG PKGVMLS DNITW A G+ D + + + E VVΞYLPL Sb_jct: 179 DTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIRPAEVQQEVVVSYLPL 238

Query: 274 SHIAAQMMDIWVPIKIGALTYFAQADALKGTLVSTLKEVKPTVFIGVPQIWEKIHEMVKK 333

SHIAAQ+ D+W 1+ GA FA+ DALKG+LV+TL+EV+PT +GVP++WEKI E +++ Sbjct: 239 SHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMERIQE 298

Query: 334 NSAKSMGLKKKAFVWARNIGFKVNSKKMLGKYNTPVSYRMAKTLVFSKVKTSLGLDHCHS 393

+A+S +++K +WA ++ + N G P + R+A LV +KV+ +LG C Sbjct: 299 VAAQSGFIRRKMLLWAMSVTLEQNLT-CPGSDLKPFTTRLADYLVLAKVRQALGFAKCQK 357

Query: 394 FISGTAPLNQETAEFFLSLDIPIGELYGLSESSGPHTISNQNNYRLLSCGKILTGCKNML 453

G AP+ ET FFL L+I + YGLSE+SGPH +S+ NYRL S GK++ GC+ L Sbjct: 358 NFYGAAPMMAETQHFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKLVPGCRVKL 417

Query: 454 FQQNKDGIGEICLWGRHIFMGYLESETETTEAIDDEGWLHSGDLGQLDGLGFLYVTGHIK 513

Q+ +GIGEICLWGR IFMGYL E +T EAID+EGWLH+GD G+LD GFLY+TG +K Sbjct: 418 VNQDAEGIGEICLWGRTIFMGYLNMEDKTCEAIDEEGWLHTGDAGRLDADGFLYITGRLK 477

Query: 514 EILITAGGENVPPIPVETLVKKKIPIISNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDK 573

E++ITAGGENVPP+P+E VK ++PI ISNAML+GD+ KFLSMLLTLKC ++ + + D Sbjct: 478 ELIITAGGENVPPVPIEEAVKMELPIISNAMLIGDQRKFLSMLLTLKCTLDPDTSDQTDN 537

Query: 574 LNFEAINFCRGLGSQASTVTEMVKQQDPLVYKAIQQGINAVNQEAMNNAQRIEKWVILEK 633

L +A+ FC+ +GS+A+TV+E+++++D VY+AI++GI VN A I+KW ILE+ Sbjct: 538 LTEQAVEFCQRVGSRATTVSEIIEKKDEAVYQAIEEGIRRVNMNAAARPYHIQKWAILER 597

Query: 634 DFSIYGGELGPMMKLKRHFVAQKYKKQIDHMY 665

DFSI GGELGP MKLKR V +KYK ID Y Sbjct: 598 DFSISGGELGPTMKLKRLTVLEKYKGIIDSFY 629

Pedant information for DKFZphtes3_35kl6, frame 2

Report for DKFZphtes3_35kl6.2

[LENGTH] 666

[MW] 74344.97

[pi] 8.67

[HOMOL] TREMBL:AB014531_1 gene: "KIAA0631" product: "KIAA0631 protein"; Homo sapiens mRNA for KIAA0631 protein, partial eds. le-176

[FUNCAT] l lipid metabolism [H. influenzae, HI0002] 2e-55

[FUNCAT] 08.10 peroxisomal transport [S. cerevisiae, YER015w] 2e-29

[FUNCAT] 30.19 peroxisomal organization [S. cerevisiae, YER015w] 2e-29

[FUNCAT] 01.06.13 lipid and fatty-acid transport [S. cerevisiae, YER015w] 2e-29

[FUNCAT] 01.06.07 lipid, fatty-acid and sterol utilization [S. cerevisiae, YER015w] 2e-29

[FUNCAT] 01.06.01 lipid, fatty-acid and sterol biosynthesis [S. cerevisiae, YMR246w] 2e-23

[FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YMR246w] 2e-23

[BLOCKS] BL00455

[SCOP] dllci 5.19.1.1.1 Luciferase [Firefly (Phontinus pyralis) le-49

[EC] 1.13.12.7 Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) 9e-17

[EC] 6.2.1.3 Long-chain-fatty-acid—CoA ligase 4e-34

[EC] 5.1.1.11 Phenylalanine racemase (ATP-hydrolysmg) 6e-08

[EC] 6.2.1.12 4-Coumarate—CoA ligase 8e-18

[PIRKW] duplication 6e-07

[PIRKW] phosphopantetheine 3e-12

[PIRKW] multifunctional enzyme 3e-06

[PIRKW] ligase 6e-08

[PIRKW] acid-thiol ligase 4e-34

[PIRKW] transmembrane protein 5e-22

[PIRKW] monooxygenase 9e-17

[PIRKW] hydrolase 4e-34

[PIRKW] peroxisome 9e-15

[PIRKW] antibiotic biosynthesis 3e-12

[PIRKW] isomerase 6e-08

[PIRKW] flavonoid biosynthesis le-17

[PIRKW] magnesium 9e-15

[PIRKW] ATP 5e-22

[PIRKW] oxidoreductase 9e-17

[PIRKW] liver 2e-31

[SUPFAM] alpha-ammoadipyl-cysteinyl-valine synthetase 3e-07

[SUPFAM] human long-chain-fatty-acid—CoA ligase 4e-34

[SUPFAM] gramicidin S synthetase I 6e-08

[SUPFAM] peptide synthetase ppsE 7e-06

[SUPFAM] gramicidin S synthetase I repeat homology 3e-12

[SUPFAM] peptide synthetase ppsD 2e-07 [SUPFAM] probable acyl-CoA ligase medium chain 2e-09

[SUPFAM] acetate—CoA ligase 8e-10

[SUPFAM] acetate—CoA ligase homology 4e-54

[SUPFAM] surfactin synthetase 3e-12

[SUPFAM] 4-coumarate—CoA ligase 8e-18

[SUPFAM] short-chain alcohol dehydrogenase homology 8e-07

[SUPFAM] acyl carrier protein homology 2e-29

[PROSITE] MYRISTYL 12

[PROSITE] AMP_BINDING 1

[PROSITE] AMIDATION 1

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE) CK2_PHOSPHO_SITE 9

[PROSITE] TYR_PHOSPHO_SITE 3

[PROSITE] PKC_PHOSPHO_SITE 10

[PROSITE] ASN_GLYCOSYLATION 2

[PFAM] AMP-binding enzymes

[KW] Irregular

[KW] 3D

[KW] LOW COMPLEXITY 1.80 %

SEQ MTGTPKTQEGAKDLEVDMNKTEVTPRLWTTCRDGEVLLRLSKHGPGHETPMTIPEFFREΞ SEG llci-

SEQ VNRFGTYPALASKNGKKWEILNFNQYYEACRKAAKΞLIKLGLERFHGVGILGFNSAEWFI SEG

SEQ TAVGAILAGGLCVGIYATNSAEACQYVITHAKVNILLVENDQQLQKILSIPQSSLEPLKA SEG

SEQ IIQYRLPMKKNNNLYSWDDFMELGRSIPDTQLEQVIESQKANQCAVLIYTSGTTGIPKGV SEG

SEQ MLSHDNITWIAGAVTKDFKLTDKHETVVSYLPLSHIAAQMMDIWVPIKIGALTYFAQADA SEG

SEQ LKGTLVSTLKEVKPTVFIGVPQIWEKIHEMVKKNSAKSMGLKKKAFVWARNIGFKVNSKK SEG llci-

SEQ MLGKYNTPVSYRMAKTLVFSKVKTSLGLDHCHSFISGTAPLNQETAEFFLSLDIPIGELY SEG llci- TTTTCEEETTTTCCCHHHHHHHHHHCCCCBCEE

SEQ GLSESSGPHTISNQNNYRLLΞCGKILTGCKNMLFQQNKDGIGEICLWGRHIFMGYLESET SEG llci- ECGGGTTEEEECCCCCCEEEEETTTTEEEEETTTTTCEETTEEEEEETTTTCCEETTTHH

SEQ ETTEAIDDEGWLHSGDLGQLDGLGFLYVTGHIKEILITAGGENVPPIPVETLVKKKIPII SEG xxxxxxxxxxxx llci- HHHHHBTTTTCEEEEEEEEETTTTCEEE ECEEETTEEECHHHHHHHHHHT-TTE

ΞEQ SNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDKLNFEAINFCRGLGSQASTVTEMVKQQD SEG llci- EEEEEEE

SEQ PLVYKAIQQGINAVNQEAMNNAQRIEKWVILEKDFSIYGGELGPMMKLKRHFVAQKYKKQ SEG

SEQ IDHMYH SEG

Prosite for DKFZphtes3_35kl6.2

PS00001 19->23 ASN_GLYCOSYLATION PDOC00001 PS00001 246->250 ASN_GLYCOSYLATION PDOC00001 PS00004 332->336 CAMP_PHOSPHO_SITE PDOC00004 PS00005 4->7 PKC_PHOSPHO_SITE PDOC00005 PS00005 24->27 PKC_PHOSPHO_SITE PDOC00005 PS00005 30->33 PKC_PHOSPHO_SITE PDOC00005 PS00005 218->221 PKC_PHOΞPHO_SITE PDOC00005 PS00005 261->264 PKC PHOSPHO SITE PDOC00005 PS00005 308->311 PKC PHOSPHO SITE PDOC00005

PS00005 335->338 PKC PHOSPHO- ^"SITE PDOC00005

PS00005 358->361 PKC PHOSPHO^{" "}SITE PDOC00005

PS00005 370->373 PKC PHOSPHO^{" "}SITE PDOC00005

PS00005 558->561 PKC PHOSPHO^{" "}SITE PDOC00005

PS00006 30->34 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 52->56 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 173->177 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 196->200 CK2 PHOSPHO^{" "}SITE PDOC00006

PΞ00006 206->210 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 210->214 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 308->312 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 478->482 CK2 PHOSPHO ^"SITE PDOC00006

PΞ00006 591->595 CK2 PHOSPHO ^"SITE PDOC00006

PS00007 659->666 TYR PHOSPHO ^"SITE PDOC00007

PS00007 658->666 TYR PHOSPHO ^"SITE PDOC00007

PS00007 597->605 TYR PHOSPHO ^"SITE PDOC00007

PS00008 3->9 MYRISTYL PDOC00008

PS00008 65->71 MYRISTYL PDOC00008

PΞ00008 124->130 MYRISTYL PDOC00008

PS00008 130->136 MYRISTYL PDOC00008

PS00008 134->140 MYRISTYL PDOC00008

PS00008 235->241 MYRISTYL PDOC00008

PS00008 239->245 MYRISTYL PDOC00008

PS00008 303->309 MYRISTYL PDOC00008

PS00008 387->393 MYRISTYL PDOC00008

PS00008 421->427 MYRISTYL PDOC00008

PS00008 498->504 MYRISTYL PDOC00008

PS00008 586->592 MYRISTYL PDOC00008

PS00009 74->78 AMIDATION PDOC00009

PS00455 227->239 AMP BINDING PDOC00427

Pfam for DKFZphtes3_35kl6.2

HMM_NAME AMP-bindmg enzymes

HMM *TYRELNERANRLARHLRsekGIrPGDιVgIMMDRSMWMIVaMLGIWKAG + + +E +A L+ +G VGI+ +S + ++ G + AG

Query 82 NFNQYYEACRKAAKSLI-KLGLERFHGVGILGFNSAEWFITAVGAILAG 129

HMM GAYVPIDPeYPdERIqYMLEDSGArLLITQrh.... HmqRIPdemwwvdH G +V I +E QY++ ++ + +L+++ + + IP++++ +

Query 130 GLCVGIYATNSAEACQYVITHAKVNILLVENDQQLQKILSIPQSSLEPLK 179

HMM IiviDWe WddlWWHedeeNpqpWvdPeDLAYIIY

+I++ + + ++++ + E ++ ++++ A +IY

Query 180 AIIQYRLPMKKNNNLYSWDDFMELGRSIPDTQLEQVIESQKANQCAVLIY 229

HMM TSGTTGKPKGVMIEHrNIvNycqWMnWRYgMteeDDRILWFtSDpYWFDa TSGTTG PKGVM++H NI+ + +++ +T+ +++ + + ++ A

Query 230 TSGTTGIPKGVMLSHDNITWIAGAVTKDFKLTDKHETVVSYLP-LSHIAA 278

HMM SVWDMFWpLLnGaTLYIpPeEtRrDPerWWqYIqRHglTWWylTPSMFRM +++D++ P+ GA Y + ++ + ++++ ++T+ ++P +++

Query 279 QMMDIWVPIKIGALTYFAQADAL—KGTLVSTLKEVKPTVFIGVPQIWEK 326

HMM LMpd

+ +

Query 327 IHEMVKKNSAKSMGLKKKAFVWARNIGFKVNSKKMLGKYNTPVSYRMAKT 376

HMM psLRhVMFgGEpLsPehWdWWRkrfgfkgRIINMYWPT

++ + +++G PL++E+++ ++ + ++I Y+ +

Query 377 LVFSKVKTSLGLDHCHSFISGTAPLNQETAEFFL-ΞLD—IPIGELYGLS 423

HMM ETTVWtTwMrliPdepeqWrwiPIGRPIpNTqWYIMDdnMQIQPiGViGE E++ T+ + + R +++G+ + + + + +N G IGE

Query 424 ESSGPHTISNQNN—Y RLLSCGKILTGCKNMLFQQN KDG-IGE 463

HMM LYIgGWPGVARGYWNRPELTEERFipNPFWPGEYRrGWNrRMYRTGDLAR +++ G ++ GY+ + +T E+ + ++ ++GDL++

Query 464 ICLWG-RHIFMGYLESETETTEAIDDEGW LHSGDLGQ 499

HMM WlPDGnlEYLGRID.DQVKIRGYRIELGEIEhqLr.qHPglqEAVV* + G+++ G I + G+++ + +E+ + ++P 1+ A

Query 500 LDGLGFLYVTGHIKEILITAGGENVPPIPVETLVKKKIPIISNAML 545 DKFZphtes3_35k24

group: transmembrane protein

DKFZphtes3_35k24 encodes a novel 514 amino ac d protein without similarity to known proteins.

The novel protein contains 5 transmembrane regions.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes and as a new marker for testicular cells. unknown ; membrane regions : 5

Summary DKFZphtes3_35k24 encodes a novel 514 amino acid protein.

No homolouges found in bacteria yeast and C. elegans, specific for mammalians? unknown complete cDNA, complete eds, few EST hits

Sequenced by DKFZ

Locus : unknown

Insert length: 2706 bp

Poly A stretch at pos. 2696, polyadenylation signal at pos. 2675

1 CCGTGTGCAG TCGCCCCGCG CCCCGCGCGA CCCTTCGGGT AAACTACGAA 51 CTGGGAGTTC TGAAGAATGG GTAAAGACTT TCGTTACTAT TTCCAGCATC

101 CCTGGTCTCG CATGATTGTG GCTTACTTGG TGATCTTCTT TAACTTCTTA

151 ATATTTGCGG AGGACCCAGT TTCTCATAGC CAAACAGAAG CCAATGTTAT

201 TGTTGTTGGA AACTGTTTTT CATTTGTTAC AAATAAATAC CCTAGAGGAG

251 TTGGCTGGAG GATTTTGAAG GTGCTTCTAT GGCTACTTGC CATTCTCACA

301 GGACTAATAG CTGGCAAATT TCTGTTCCAT CAGCGTTTGT TTGGTCAGTT

351 GCTCCGATTA AAAATGTTTC GAGAAGATCA TGGGTCGTGG ATGACAATGT

401 TCTTCAGCAC AATTCTCTTT CTCTTCATAT TTTCTCACAT ATACAACACG

451 ATTCTTCTAA TGGATGGGAA CATGGGAGCA TATATCATTA CAGACTATAT

501 GGGCATCCGA AATGAAAGTT TCATGAAATT AGCTGCAGTA GGGACCTGGA

551 TGGGGGACTT TGTCACAGCT TGGATGGTCA CTGATATGAT GCTTCAGGAC

601 AAACCCTATC CTGACTGGGG AAAATCAGCA AGAGCTTTCT GGAAGAAAGG

651 AAATGTTAGG ATCACTTTAT TCTGGACAGT TCTTTTTACT CTGACGTCTG

701 TGGTTGTACT TGTGATTACA ACGGACTGGA TCAGCTGGGA CAAGCTGAAT

751 CGGGGATTTT TGCCCAGTGA TGAAGTTTCC AGAGCATTCC TTGCTTCTTT

801 TATCTTGGTC TTTGACCTTC TTATTGTGAT GCAGGACTGG GAATTCCCAC

851 ATTTCATGGG AGATGTTGAT GTAAATCTCC CTGGTTTGCA CACCCCTCAC

901 ATGCAGTTCA AGATTCCTTT CTTCCAGAAA ATCTTCAAGG AGGAATATCG

951 TATTCACATA ACAGGCAAAT GGTTTAACTA TGGAATTATC TTCCTCGTCT 1001 TGATTTTGGA TCTTAATATG TGGAAGAACC AAATATTTTA TAAACCTCAT 1051 GAATATGGGC AATATATCGG CCCGGGGCAG AAGATATATA CAGTGAAAGA 1101 CTCAGAAAGT TTAAAAGATT TGAACAGAAC CAAGCTATCC TGGGAATGGA 1151 GGTCCAATCA CACTAACCCT CGGACTAATA AAACATATGT TGAGGGAGAC 1201 ATGTTCTTAC ACAGCAGGTT CATAGGAGCC AGTCTTGATG TCAAGTGTCT 1251 GGCCTTTGTT CCAAGCCTGA TAGCCTTTGT GTGGTTTGGA TTCTTTATTT 1301 GGTTCTTTGG ACGATTTTTG AAAAATGAGC CACGCATGGA GAATCAAGAC 1351 AAAACTTACA CTCGCATGAA AAGAAAATCT CCATCAGAAC ATAGCAAAGA 1401 CATGGGAATC ACTCGAGAAA ACACCCAGGC TTCAGTAGAA GACCCCTTGA 1451 ATGACCCTTC TTTGGTTTGC ATCAGGTCTG ACTTCAATGA GATCGTCTAC 1501 AAGTCTTCCC ACCTAACCTC GGAAAACTTG AGCTCACAGT TGAACGAATC 1551 TACTAGTGCA ACAGAAGCTG ATCAAGACCC AACGACTTCT AAAAGTACAC 1601 CTACGAACTA GACTCGGAGA TAGACTTGGA GATAACACAA AAAGCAACCT 1651 TGAGTGTAAC TTTAAAAATT TAGTCTTTCC TTTTGTATAT GTAAGGTTTA 1701 CGTAGTGTTA GGTAAAAATA TGAACAATGC CACAACGGTG CTCAACATGC 1751 TTTTTCTAGG ATTCATTGTT TTCTATTTGT ATTATAATAC ACGTGCCTAC 1801 TGTATACTCA ACAGTCCTCT AGAGATTGCT TTTCACAATT GCACAAGCTA 1851 TTACTGACTT TACAGCATAG TGGAAGATTA GCTGATGACC CATGTATCTG 1901 ATGTTCAACC ATAGTGGTGC CTTGAGACAT TAAACTGTTT TTAACTGTAC 1951 CAGAAATGAA GTGTGGAACA GTTACCTAAC CTATTTCACA TGGGCGTTTT 2001 GTATACAACT ATTTTGATCT ACACTTGATG TCTGAGCAGA AAACAGAAAT 2051 AGCTAAATGT GACTCAGGAA GTATCTCTTG GTTTCTTATT CAGCAGCAGA 2101 GTTGGTGACT TTGACAACTG GACTGCAGAG AAACATGGTG ATCACCTTTT 2151 AATTTTTATT GGCTGTCTGC CAAATATAAA TACAGATGCA AAATTCAGTA 2201 ATAGGAGATC CATAACCCAA CATGGGTCAC TACTCGTGAA ATGTGACTTT 2251 CTCCCACCAG TAATTGAAAT GAGGTGATGA TACCTAATTA TGTTTTCCTA 2301 ATTAAAGATA AATTGCTACT TGATTAAAAA TCCTGCCCTT CACCTTTGGG 2351 AACAAAGGTT AAGAGACACA GTTGGGCGAA CTCTCAAATT TATTGGCATT

2401 TACACAAAGT CCCAGACAAC CAAGGAACTG AAGTTTTCAT CATATGAGAG

2451 CAGCACATCC CACCATTTAC AATATTCGTA TATCTTTCTG CAAATATGGC

2501 TCTGGATAGT GAAAATTGAA AAACATATGC CAACCCTGAG CAAGGGAACT

2551 CCTCAAAAAA TCATGCAGCG GAACCTTGTC AGGTAGAGAA GCCGTGCATG

2601 AAAGAATTTG TTTAATGTCT TGTTTTGCGT ATGTGTTTTT TGTTTTTGTT

2651 TTTTAAGAAC TAAATATTGC ACATTAATAA ATAAGAATTA TACAGCAAAA

2701 AAAAAA

BLAST Results

No BLAST result

Medlme entries

No Medline entry

Peptide information for frame 1

ORF from 67 bp to 1608 bp; peptide length: 514 Category: putative protein

1 MGKDFRYYFQ HPWSRMIVAY LVIFFNFLIF AEDPVSHSQT EANVIWGNC

51 FSFVTNKYPR GVGWRILKVL LWLLAILTGL IAGKFLFHQR LFGQLLRLKM

101 FREDHGSWMT MFFSTILFLF IFSHIYNTIL LMDGNMGAYI ITDYMGIRNE

151 SFMKLAAVGT WMGDFVTAWM VTDMMLQDKP YPDWGKSARA FWKKGNVRIT

201 LFWTVLFTLT ΞVVVLVITTD WISWDKLNRG FLPSDEVSRA FLASFILVFD

251 LLIVMQDWEF PHFMGDVDVN LPGLHTPHMQ FKIPFFQKIF KEEYRIHITG

301 KWFNYGIIFL VLILDLNMWK NQIFYKPHEY GQYIGPGQKI YTVKDSESLK

351 DLNRTKLSWE WRSNHTNPRT NKTYVEGDMF LHSRFIGASL DVKCLAFVPS

401 LIAFVWFGFF IWFFGRFLKN EPRMENQDKT YTRMKRKSPS EHSKDMGITR

451 ENTQASVEDP LNDPSLVCIR SDFNEIVYKS SHLTSENLSS QLNESTSATE

501 ADQDPTTSKS TPTN

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_35k24, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphtes3_35k24, frame 1

Report for DKFZphtes3_35k24.1

[LENGTH] 514

[MW] 60185.03

[pi] 8.67

[PROSITE] MYRISTYL 5

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 8

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 7

[PROSITE] ASN_GLYCOSYLATION 6

[KW] ΞIGNAL_PEPTIDE 32

[KW] TRANSMEMBRANE 5

[KW] LOW COMPLEXITY 15.37 %

SEQ MGKDFRYYFQHPWSRMIVAYLVIFFNFLIFAEDPVSHΞQTEANVIVVGNCFΞFVTNKYPR

SEG

PRD cccceeeeeecccchhhhhhhhhhhhhhhhccccccccccceeeeeecccceeeeccccc

MEM

SEQ GVGWRILKVLLWLLAILTGLIAGKFLFHQRLFGQLLRLKMFREDHGSWMTMFFSTILFLF

SEG xxxxxxxxxxxxxxxxx xxxxxxxxxxxx

PRD cchhhhhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhccccceeeehhhhhhhh

MEM MMMMMMMMMMMMMMMMM MMMMM SEQ IFSHIYNTILLMDGNMGAYIITDYMGIRNESFMKLAAVGTWMGDFVTAWMVTDMMLQDKP

SEG xxx

PRD hhhhhhhhhhccccccceeeeecccccchhhhhhhhhhccccccccchhhhhhhhhhccc

MEM MMMMMMMMMMMM

SEQ YPDWGKSARAFWKKGNVRITLFWTVLFTLTSVVVLVITTDWISWDKLNRGFLPSDEVSRA

SEG xxxxxxxxxxxxxxxxxxxxx

PRD cccccchhhhhhhcccceeehhhhhhhhhhhheeeeecccccccccccccccccchhhhh

MEM MMMMMMMMMMMMMMMMM M

SEQ FLASFILVFDLLIVMQDWEFPHFMGDVDVNLPGLHTPHMQFKIPFFQKIFKEEYRIHITG

SEG xxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhcccccccccccccccccccccccccchhhhhhhhhhhhhhcccc

MEM MMMMMMMMMMMMMMMM

SEQ KWFNYGIIFLVLILDLNMWKNQIFYKPHEYGQYIGPGQKIYTVKDSESLKDLNRTKLSWE

SEG

PRD ccceeeeeehhhhhhhcccccceeeccccccccccccceeeeecccccccccccchhhhh

MEM

SEQ WRSNHTNPRTNKTYVEGDMFLHSRFIGASLDVKCLAFVPSLIAFVWFGFFIWFFGRFLKN

SEG xxxxxxxxxxxxxx ...

PRD hhcccccccccccccccchhhhhhccccccceeeeeehhhhheeeeccceeeeeeeeccc

MEM MMMMMMMMMMMMMMMMM

SEQ EPRMENQDKT TRMKRKSPSEHSKDMGITRENTQASVEDPLNDPSLVCIRSDFNEIVYKS

SEG

PRD cccccccccchhhhhhccccccccccceeeccccccccccccccceeeeccccceeeeec

MEM

SEQ SHLTSENLSSQLNESTSATEADQDPTTSKSTPTN

SEG

PRD cccccccccccccccccccccccccccccccccc

MEM

Prosite for DKFZphtes3_35k24.1

PS00001 149->153 ASN_GLYCOSYLATION PDOC00001

PS00001 353->357 ASN_GLYCOSYLATION PDOC00001

PS00001 364->368 ASN_GLYCOSYLATION PDOC00001

PΞ00001 371->375 ASN_GLYCOSYLATION PDOC00001

PS00001 487->491 ASN_GLYCOSYLATION PDOC00001

PS00001 493->497 ASN_GLYCOSYLATION PDOC00001

PS00004 435->439 CAMP_PHOSPHO_SITE PDOC00004

PS00005 55->58 PKC_PHOSPHO_SITE PDOC00005

PS00005 187->190 PKC_PHOSPHO_SITE PDOC00005

PS00005 299->302 PKC_PHOSPHO_SITE PDOC00005

PS00005 342->345 PKC_PHOSPHO_SITE PDOC00005

PS00005 348->351 PKC_PHOΞPHO_SITE PDOC00005

PS00005 370->373 PKC_PHOSPHO_SITE PDOC00005

PS00005 507->510 PKC_PHOSPHO_SITE PDOC00005

PS00006 38->42 CK2_PHOSPHO_SITE PDOC00006

PS00006 342->346 CK2_PHOSPHO_SITE PDOC00006

PS00006 348->352 CK2_PHOSPHO_SITE PDOC00006

PS00006 373->377 CK2_PHOSPHO_SITE PDOC00006

PS00006 438->442 CK2_PHOSPHO_SITE PDOC00006

PS00006 456->460 CK2_PHOSPHO_SITE PDOC00006

PS00006 497->501 CK2_PHOSPHO_SITE PDOC00006

PS00006 499->503 CK2_PHOSPHO_SITE PDOC00006

PS00007 326->334 TYR_PHOSPHO_SITE PDOC00007

PS00008 48->54 MYRISTYL PDOC00008

PS00008 79->85 MYRISTYL PDOC00008

PS00008 106->112 MYRISTYL PDOC00008

PS00008 134->140 MYRISTYL PDOC00008

PS00008 159->165 MYRISTYL PDOC00008

(No Pfam data available for DKFZphtes3_35k24.1) DKFZphtes3_35nl2 group: metabolism

DKFZphtes3_35nl2 encodes a novel 315 amino acid protein with strong similarity to ADP, ATP carrier T (ANT) proteins.

The novel protein contains three mitochondrial energy transfer signatures and is closely related to the ADP/ATP translocator, or ademne nucleotide translocator (ANT) , a protein most abundant in mitochondria. In its functional state, it is a homodimer of 30-kD subunits embedded asymmetrically m the inner mitochondrial membrane. The dimer forms a gated pore through which ADP is moved from the matrix into the cytoplasm.

The new protein can find application in modulation of ADP-transport and energy metabolism in cells/mitochondria . strong similarity to ADP/ATP carrier proteins

EST hits to mouse and drosophila

Sequenced by DKFZ

Locus : unknown

Insert length: 1803 bp

Poly A stretch at pos. 1793, polyadenylation signal at pos. 1772

1 AGCGTCCCAA GAGCCACTTT CTCGCCAGTA CGATGCTGCA GCGGTTTTCC

51 GGTTTTCCGC TTCCCTTCAT CGTAGCTCCC GTACTCATTT TTAGCCACTG

101 CTGCCGGTTT TTATATCCTT CTCCATCATG CATCGTGAGC CTGCGAAAAA

151 GAAGGCAGAA AAGCGGCTGT TTGACGCCTC ATCCTTCGGG AAGGACCTTC

201 TGGCCGGCGG AGTCGCGGCA GCTGTGTCCA AGACAGCGGT GGCGCCCATC

251 GAGCGGGTGA AGCTGCTGCT GCAGGTGCAG GCGTCGTCGA AGCAGATCAG

301 CCCCGAGGCG CGGTACAAAG GCATGGTGGA CTGCCTGGTG CGGATTCCTC

351 GCGAGCAGGG TTTCTTCAGT TTTTGGCGTG GCAATTTGGC AAATGTTATT

401 CGGTATTTTC CAACACAAGC TCTAAACTTT GCTTTTAAGG ACAAATACAA

451 GCAGCTATTC ATGTCTGGAG TTAATAAAGA AAAACAGTTC TGGAGGTGGT

501 TTTTGGCAAA CCTGGCTTCT GGTGGAGCTG CTGGGGCAAC ATCCTTATGT

551 GTAGTATATC CTCTAGATTT TGCCCGAACC CGATTAGGTG TCGATATTGG

601 AAAAGGTCCT GAGGAGCGAC AATTCAAGGG TTTAGGTGAC TGTATTATGA

651 AAATAGCAAA ATCAGATGGA ATTGCTGGTT TATACCAAGG GTTTGGTGTT

701 TCAGTACAGG GCATCATTGT GTACCGAGCC TCTTATTTTG GAGCTTATGA

751 CACAGTTAAG GGTTTATTAC CAAAGCCAAA GAAAACTCCA TTTCTTGTCT

801 CCTTTTTCAT TGCTCAAGTT GTGACTACAT GCTCTGGAAT ACTTTCTTAT

851 CCCTTTGACA CAGTTAGAAG ACGTATGATG ATGCAGAGTG GTGAGGCTAA

901 ACGGCAATAT AAAGGAACCT TAGACTGCTT TGTGAAGATA TACCAACATG

951 AAGGAATCAG TTCCTTTTTT CGTGGCGCCT TCTCCAATGT TCTTCGCGGT

1001 ACAGGGGGTG CTTTGGTGTT GGTATTATAT GATAAAATTA AAGAATTCTT

1051 TCATATTGAT ATTGGTGGTA GGTAATCGGG AGAGTAAATT AAGAAATAAC

1101 ATGGATTTAA CTTGTTAAAC ATACAAATTA CATAGCTGCC ATTTGCATAC

1151 ATTTTGATAG TGTTATTGTC TGTATTTTGT TAAAGTGCTA GTTCTGCAAT

1201 AAAGCATACA TTTTTTCAAG AATTTAAATA CTAAAAATCA GATAAATGTG

1251 GATTTTCCTC CCACTTAGAC TCAAACACAT TTTAGTGTGA TATTTCATTT

1301 ATTATAGGTA GTATATTTTA ATTTGTTAGT TTAAAATTCT TTTTATGATT

1351 AAAAATTAAT CATATAATCC TAGATTAATG CTGAAATCTA GGAAATGAAA

1401 GTAGCGTCTT TTAAATTGCT ATTCATTTAA TATACCTGTT TTCCCATCTT

1451 TTGAAGTCAT ATGGTATGAC ATATTTCTTA AAAGCTTATC AATAGATGTC

1501 ATCATATGTG TAGGCAGAAA TAAGCTTTGT TCTATATCTC TTCTAAGACA

1551 GTTGTTATTA CTGTGTATAA TATTTACAGT ATCAGCCTTT GATTATAGAT

1601 GTGATCATTT AAAATTTGAT AATGACTTTA GTGACATTAT AAAACTGAAA

1651 CTGGAAAATA AAATGGCTTA TCTGCTGATG TTTATCTTTA AAATAAATAA

1701 AATCTTGCTA GTGTGAATAT ATCTTAGAAC AAAAGGTATC CTCTTGAAAA

1751 TTAGTTTGTA TATTTTGTTG ACAATAAAGG AAGCTTAACT GTTAAAAAAA

1801 AAA

BLAST Results

No BLAST result

Medline entries

96289608:

Molecular biological and quantitative abnormalities of

ADP/ATP carrier protein in cardiomyopathic hamsters. Peptide information for frame 2

ORF from 128 bp to 1072 bp; peptide length: 315

Category: strong similarity to known protein

Classification: Metabolism

Prosite motifs: MITOCH_CARRIER (40-50)

MITOCH_CARRIER (145-155)

MITOCH CARRIER (242-252)

1 MHREPAKKKA EKRLFDASSF GKDLLAGGVA AAVSKTAVAP IERVKLLLQV 51 QASSKQISPE ARYKGMVDCL VRIPREQGFF SFWRGNLANV IRYFPTQALN 101 FAFKDKYKQL FMSGVNKEKQ FWRWFLANLA SGGAAGATSL CVVYPLDFAR 151 TRLGVDIGKG PEERQFKGLG DCIMKIAKSD GIAGLYQGFG VSVQGIIVYR 201 ASYFGAYDTV KGLLPKPKKT PFLVSFFIAQ VVTTCSGILS YPFDTVRRRM 251 MMQSGEAKRQ YKGTLDCFVK IYQHEGISSF FRGAFSNVLR GTGGALVLVL 301 YDKIKEFFHI DIGGR

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_35nl2, frame 2

PIR:S37210 ADP, ATP carrier protein TI - mouse, N = 1, Score = 1127, P = 2.7e-114

PIR:A44778 ADP, ATP carrier protein TI - human, N = 1, Score = 1125, P = 4.4e-114

TREMBL :DMADPATPT_2 product: "ADP/ATP translocase"; Drosophila melanogaster gene encoding ADP/ATP translocase, N = 1, Score = 1124, P = 5.6e-114

PIR:XWBO ADP, ATP carrier protein TI - bovine, N = 1, Score = 1121, P = 1.2e-113

>PIR:S37210 ADP, ATP carrier protein TI - mouse Length = 298

HSPs:

Score = 1127 (169.1 bits), Expect = 2.7e-114, P = 2.7e-114 Identities = 214/293 (73%), Positives = 248/293 (84%)

Query: 17 ASSFGKDLLAGGVAAAVSKTAVAPIERVKLLLQVQASSKQISPEARYKGMVDCLVRIPRE 76

A SF KD LAGG+AAAVSKTAVAPIERVKLLLQVQ +SKQIS E +YKG++DC+VRIP+E Sbjct: 5 ALSFLKDFLAGGIAAAVSKTAVAPIERVKLLLQVQHASKQISAEKQYKGIIDC VRIPKE 64

Query: 77 QGFFΞFWRGNLANVIRYFPTQALNFAFKDKYKQLFMSGVNKEKQFWRWFLANLASGGAAG 136

QGF SFWRGNLANVIRYFPTQALNFAFKDKYKQ+F+ GV++ KQFWR+F NLASGGAAG Sbjct: 65 QGFLSFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDRHKQFWRYFAGNLASGGAAG 124

Query: 137 ATSLCVVYPLDFARTRLGVDIGKGPEERQFKGLGDCIMKIAKSDGIAGLYQGFGVSVQGI 196

ATSLC VYPLDFARTRL D+GKG +R+F GLGDC+ KI KSDG+ GLYQGF VSVQGI Sbjct: 125 ATSLCFVYPLDFARTRLAADVGKGSSQREFNGLGDCLTKIFKSDGLKGLYQGFSVSVQGI 184

Query: 197 IVYRASYFGAYDTVKGLLPKPKKTPFLVSFFIAQVVTTCSGILSYPFDTVRRRMMMQSGE 256

I+YRA+YFG YDT KG+LP PK +VS+ IAQ VT +G++SYPFDTVRRRMMMQSG Sbjct: 185 IIYRAAYFGVYDTAKGMLPDPKNVHIIVSWMIAQSVTAVAGLVSYPFDTVRRRMMMQSGR 244

Query: 257 —AKRQYKGTLDCFVKIYQHEGISSFFRGAFSNVLRGTGGALVLVLYDKIKEF 307

A Y GTLDC+ KI + EG ++FF+GA+SNVLRG GGA VLVLYD+IK++ Sbjct: 245 KGADIMYTGTLDCWRKIAKDEGANAFFKGAWΞNVLRGMGGAFVLVLYDEIKKY 297

Pedant information for DKFZphtes3_35nl2, frame 2

Report for DKFZphtes3_35nl2.2

[LENGTH] 315 [MW] 35022.03

[pi] 9.91

[HOMOL] PIR:S37210 ADP, ATP carrier protein TI - mouse le-115

[FUNCAT] 07.16 purine and pyrimidme transporters [S. cerevisiae, YBL030c] 2e-72

[FUNCAT] 08.04 mitochondrial transport [S. cerevisiae, YBL030c] 2e-72

[ FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YBL030c] 2e-72

[FUNCAT] 01.03.19 nucleotide transport [S. cerevisiae, YBL030c] 2e-72

[FUNCAT] 01.07.10 transport of vitamins, cofactors, and prosthetic groups [S. cerevisiae, YIL006w] 2e-14

[FUNCAT] 07.99 other transport facilitators [S. cerevisiae, YIL006w] 2e-14

[FUNCAT] 01.05.07 carbohydrate transport [S. cerevisiae, YPR021c] 5e-14

[FUNCAT] 07.07 sugar and carbohydrate transporters [Ξ. cerevisiae, YPR021C] 5e-14

[FUNCAT] 07.04.07 amon transporters (cl, so4, po4, etc.) [S. cerevisiae, YKL120w] le-13

[FUNCAT] 02.13 respiration [Ξ. cerevisiae, YBR192w] 4e-13

[FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YJR095w]

6e-12

[FUNCAT] 13.04 homeostasis of other ions [S. cerevisiae, YLR348c] 4e-10

[FUNCAT] 01.04.07 phosphate transport [S. cerevisiae, YLR348c] 4e-10

[FUNCAT] 01.01.07 ammo-acid transport [S. cerevisiae, YOR130C] le-06

[FUNCAT] 07.10 amino-acid transporters [S. cerevisiae, YOR130c] le-06

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YPR128c] 2e-06

[FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YKR052c] 2e-06

[BLOCKS] BL00215B Mitochondrial energy transfer proteins

[BLOCKS] BL00215A Mitochondrial energy transfer proteins

[PIRKW] duplication le-115

[PIRKW] phosphate transport 2e-09

[PIRKW] heart 3e-24

[PIRKW] transmembrane protein le-115

[PIRKW] mitochondrial inner membrane 7e-72

[PIRKW] transport protein 4e-08

[PIRKW] acetylated amino end le-115

[PIRKW] adipose tissue 5e-13

[PIRKW] mitochondrion le-115

[PIRKW] alternative splicing 2e-09

[PIRKW] methylated ammo acid le-115

[PIRKW] chloroplast 2e-14

[PIRKW] homodimer le-115

[SUPFAM] hypothetical protein YFR045w 3e-07

[SUPFAM] ADP, ATP carrier protein le-115

[SUPFAM] Btl protein 2e-14

[SUPFAM] ADP, ATP carrier protein repeat homology le-115

[SUPFAM] probable carrier protein YPR021c le-12

[PROSITE] MITOCH_CARRIER 3

[PFAM] Mitochondrial carrier proteins

[KW] TRANSMEMBRANE 2

[KW] LOW COMPLEXITY 4.76 %

SEQ MHREPAKKKAEKRLFDASSFGKDLLAGGVAAAVSKTAVAPIERVKLLLQVQASSKQISPE SEG PRD ccchhhhhhhhhhhhhchhhhhhhhhchhhhhhhhhhhcchhhhhhhhhhhhhhhhhhhh

MEM

SEQ ARYKGMVDCLVRIPREQGFFSFWRGNLANVIRYFPTQALNFAFKDKYKQLFMSGVNKEKQ SEG PRD hhhhhhhheeeeccccceeeeecccccceeeeecccchhhhhhhhhhhhhhccccccccc

MEM

SEQ FWRWFLANLASGGAAGATSLCVVYPLDFARTRLGVDIGKGPEERQFKGLGDCIMKIAKSD SEG xxxxxxxxxxxxxx PRD eeeecccccccccccceeeeeeeccchhhhhhhhhhccccchhhhhhcccceeeeeeccc MEM

SEQ GIAGLYQGFGVSVQGIIVYRASYFGAYDTVKGLLPKPKKTPFLVSFFIAQVVTTCSGILS SEG PRD cccccccccceeeccceeehhhhhccccccccccccccccccchhhhhhhhhhheeeeec

MEM .... MMMMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMM

SEQ YPFDTVRRRMMMQSGEAKRQYKGTLDCFVKIYQHEGISSFFRGAFSNVLRGTGGALVLVL SEG PRD cccchhhhhhhhhcccceeeecccchhhhhhhhhcccccccccchhhhhccccceeeeee MEM MMMMMMMMMMM

SEQ YDKIKEFFHIDIGGR SEG PRD hhhhhhheeeecccc MEM Prosite for DKFZphtes3_35nl2.2

PS00215 40->50 MITOCH_CARRIER PDOC00189 PS00215 145->155 MITOCH_CARRIER PDOC00189 PS00215 242->252 MITOCH CARRIER PDOC00189

Pfam for DKFZphtes3_35nl2.2

HMM_NAME Mitochondrial carrier proteins

HMM *pFwkdFLAGGIAGmMeHTvMFPIDtIKTRMQlQgEMpM.. ahpRYkGMI +F+KD+LAGG+A+++++T+++PI+++K+++Q+Q +++ RYKGM+

Query 19 SFGKDLLAGGVAAAVSKTAVAPIERVKLLLQVQASSKQISPEARYKGMV 67

HMM dCFRwIwkNEGWRGLWRGLgANvIRYIPqWalRFGFYEFMKeMFiDyfge DC+ +I++++G++++WRG++ANVIRY+P++A++F+F++ +K +F + +++

Query 68 DCLVRIPREQGFFSFWRGNLANVIRYFPTQALNFAFKDKYKQLFMSGVNK 117

HMM ddnyWmWFwmnYMaGsmAGEwisvIitYPMWvVKTRLQaDqkHphsQp . R ++W+WF+ N+++G++AG ++S+ ++YP+++++TRL D +++++ R

Query 118 EKQFWRWFLANLASGGAAG-ATSLCVVYPLDFARTRLGVD--IGKGPEER 164

HMM hYNGvWNcWrklYReEGgFkGLYRGWtPTWMRMIPYqmiYFfvYEtLKeW +++G+ +C KI +++G ++GLY+G++ +++++I+Y++ YF++Y+T K +

Query 165 QFKGLGDCIMKIAKSDG-IAGLYQGFGVΞVQGIIVYRAΞYFGAYDTVKGL 213

HMM lynYtgYnPgprelCMddsPwWhWiIgWmlAGMiaWivSYPfDVVRTRMM L +++ + ++++++I++ ++ ++++I+SYPFD+VR+RMM

Query 214 LP KPK—KTPFLVSFFIAQVVT-TCSGILSYPFDTVRRRMM 251

HMM Mdsm.edhkYqSmlDCWMqIYKnEGFkGFWKGFWPRIMRiMPWtAIMFml

M+S+ ++++Y+++LDC+++IY++EG+ +F++G+ +++R+ ++A+++++

Query 252 MQSGEAKRQYKGTLDCFVKIYQHEGISSFFRGAFSNVLRGT-GGALVLVL 300

HMM YEqMKwFL* Y+ +K+F+

Query 301 YDKIKEFF 308

DKFZphtes3 35n24

group: testes derived

DKFZphtes3_35n24 encodes a novel 365 amino acid protein without similarity to known proteins.

The novel protein contains a Prosite Ig (Immunoglubulm) -MHC pattern. This pattern represents a domain, approximately one hundred amino acids long and including a conserved mtra-domain disulfide bond (llg domaini) . Thus, the novel protein is a new member of the Ig-superfamily. No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, EST hits

Sequenced by DKFZ

Locus: unknown

Insert length: 1589 bp

Poly A stretch at pos. 1579, polyadenylation signal at pos. 1560

1 CGATCGTCAC GTGACGCCGG GGTTCAGCGT ATCCTTGCTG GGCAACCGTC 51 TTAGAGACCA GCACTGCTGG CTGCACCATG AATGTGATCT ACCCACTGGC

101 AGTCCCCAAG GGGCGCAGAC TCTGCTGTGA GGTGTGCGAA GCCCCAGCCG

151 AGCGGGTGTG CGCGGCCTGC ACAGTCACTT ATTACTGTGG GGTGGTACAT

201 CAGAAGGCTG ACTGGGACAG CATCCATGAG AAAATATGTC AGCTCTTGAT

251 TCCACTGCGC ACTTCCATGC CCTTCTACAA TTCAGAGGAA GAACGGCAGC

301 ATGGCCTGCA GCAGCTGCAG CAGCGGCAGA AGTATTTGAT TGAATTCTGC

351 TACACCATAG CCCAGAAATA CCTCTTTGAA GGGAAACACG AAGATGCTGT

401 ACCAGCAGCT TTGCAGTCCC TTCGCTTCCG TGTGAAGCTG TATGGCCTGA

451 GCTCCGTAGA GCTTGTGCCT GCTTACCCGC TGTTGGCCGA GGCCAGCCTT

501 GGTCTGGGCC GAATCGTTCA GGCTGAAGAA TATCTATTCC AAGCCCAGTG

551 GACAGTCCTC AAATCAACTG ACTGTAGTAA TGCCACCCAC TCTTTACTGC

601 ATCGGAATCT GGGACTTCTC TATATAGCTA AGAAAAACTA TGAAGAGGCC

651 CGTTATCATC TGGCCAATGA TATTTATTTT GCCAGTTGTG CATTTGGAAC

701 AGAGGACATT AGGACTTCAG GAGGCTACTT CCACCTGGCT AATATATTCT

751 ATGACCTTAA AAAGTTGGAC CTGGCAGACA CATTGTACAC CAAGGTCTCT

801 GAGATCTGGC ATGCATATTT GAACAATCAC TATCAAGTCC TCTCACAGGC

851 TCACATCCAA CAAATGGATT TACTGGGCAA ACTATTTGAG AATGACACTG

901 GCTTGGATGA AGCCCAAGAA GCAGAAGCCA TTCGCATCCT GACTTCAATC

951 TTGAACATTC GAGAATCTAC ATCTGACAAA GCCCCCCAAA AAACCATCTT 1001 TGTTCTGAAG ATCCTGGTCA TGCTTTACTA CCTGATGATG AATTCTTCAA 1051 AGGCACAGGA ATATGGCATG AGGGCCCTCA GTCTAGCCAA AGAACAACAG 1101 CTTGATGTCC ATGAGCAAAG CACCATTCAA GAGTTATTAA GTCTCATTTC 1151 AACTGAAGAC CATCCCATTA CTTAGTGACC CATGAGCTCT GCATCAAGGG 1201 TTATTCCAGG GGCTACTGAA GATCTAATAT ATTCCAGCCT TGCACAACTG 1251 CTTTGAGGTA CTGTAGACTG CTGAAGTTTC CACCCTCTTC CCCTGGGATT 1301 GCACACATAG CTGTTATTTT TTTCTTACAC AGCATATTAA GGGAATATAA 1351 AGCTTTAGGC ATAGAAATCA CTAAAAACTG TGTTTGTCAT GACCTTTGTA 1401 CTTGATTTAT CATGACTTTG TATGACTGAG TAATATGTAG TCAGATCACT 1451 AATATGGTAT TTGTAATTAA ACTACAAATA GTTTGTCATT TCCCAGAAGT 1501 CTTCCAACGA TGCATGTTTC ATACACTTTT GCTAAAGGAG GGGTAAAGGA 1551 GGGGGTAGGG AATAAAGCTA TATTGGAACA AAAAAAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 78 bp to 1172 bp; peptide length: 365 Category: putative protein Prosite motifs: IG MHC (35-42)

1 MNVIYPLAVP KGRRLCCEVC EAPAERVCAA CTVTYYCGVV HQKADWDSIH 51 EKICQLLIPL RTSMPFYNΞE EERQHGLQQL QQRQKYLIEF CYTIAQKYLF 101 EGKHEDAVPA ALQSLRFRVK LYGLSSVELV PAYPLLAEAS LGLGRIVQAE 151 EYLFQAQWTV LKSTDCSNAT HSLLHRNLGL LYIAKKNYEE ARYHLANDIY 201 FASCAFGTED IRTSGGYFHL ANIFYDLKKL DLADTLYTKV SEIWHAYLNN 251 HYQVLSQAHI QQMDLLGKLF ENDTGLDEAQ EAEAIRILTS ILNIRESTSD 301 KAPQKTIFVL KILVMLYYLM MNSSKAQEYG MRALSLAKEQ QLDVHEQSTI 351 QELLSLISTE DHPIT

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_35n24, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphtes3_35n24, frame 3

Report for DKFZphtes3_35n24.3

[LENGTH] 365

[MW] 41768.24

[pi] 5.82

[BLOCKS] BL00273 Heat-stable enterotoxins proteins

[PROSITE] MYRISTYL 1

[PROSITE] IG_MHC 1

[PROSITE] AMIDATION 1

[PROSITE] CK2_PHOSPHO_SITE 7

[PROSITE] TYR_PHOSPHO_SITE 4

[PROSITE] PKC_PHOSPHO_SITE 3

[PROSITE] ASN_GLYCOSYLATION 3

[KW] Alpha_Beta

[KW] LOW_COMPLEXITY 4.11 %

SEQ MNVIYPLAVPKGRRLCCEVCEAPAERVCAACTVTYYCGVVHQKADWDSIHEKICQLLIPL SEG PRD ccceeeeeccccceeeeeeeehhhhhhhheeeeeeeeeecccccccchhhhhhhhheeec

SEQ RTSMPFYNSEEERQHGLQQLQQRQKYLIEFCYTIAQKYLFEGKHEDAVPAALQSLRFRVK SEG xxxxxxxxxxxxxxx PRD cccccccchhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhcccccccchhhhhhhhhhhh

SEQ LYGLSSVELVPAYPLLAEASLGLGRIVQAEEYLFQAQWTVLKSTDCSNATHSLLHRNLGL SEG PRD hhccceeeeccccchhhhhccccchhhhhhhhhhhhhhhccccccccccccccccccccc

SEQ LYIAKKNYEEARYHLANDIYFASCAFGTEDIRTSGGYFHLANIFYDLKKLDLADTLYTKV SEG PRD eeeehhhhhhhhhhhhhheeeeeccccccccccccceeehhhhhhhhhhhhccceeeeeh

SEQ SEIWHAYLNNHYQVLSQAHIQQMDLLGKLFENDTGLDEAQEAEAIRILTSILNIRESTSD SEG PRD hhhhhhhhcccchhhhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhhhccccc

SEQ KAPQKTIFVLKILVMLYYLMMNSSKAQEYGMRALSLAKEQQLDVHEQSTIQELLSLISTE SEG PRD ccccceeeehhhhhhhhhhhhcccchhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhhcc

SEQ DHPIT SEG PRD ccccc

Prosite for DKFZphtes3_35n24.3

PS00001 168->172 ASN_GLYCOSYLATION PDOC00001

PS00001 272->276 ASN_GLYCOSYLATION PDOC00001

PS00001 322->326 ASN_GLYCOSYLATION PDOC00001

PS00005 114->117 PKC_PHOSPHO_SITE PDOC00005

PS00005 299->302 PKC_PHOSPHO_SITE PDOC00005

PS00005 323->326 PKC PHOSPHO SITE PDOC00005 PS00006 48->52 CK2 PHOSPHO SITE PDOC00006

PS00006 69->73 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 125->129 CK2 PHOSPHO^" SITE PDOC00006

PS00006 274->278 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 297->301 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 349->353 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 358->362 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00007 85->93 TYR PHOSPHO ^"SITE PDOC00007

PS00007 186->194 TYR PHOSPHO^{" "}SITE PDOC00007

PS00007 186->194 TYR PHOSPHO ^"SITE PDOC00007

PS00007 185->194 TYR PHOSPHO^{" "}SITE PDOC00007

PS00008 275->281 MYRISTYL PDOC00008

PS00009 11->15 AMIDATION PDOC00009

PΞ00290 35->42 IG MHC PDOC00262

(No Pfam data available for DKFZphtes3_35n24.3)

DKFZphtes3_35n9 group: metabolism

DKFZphftes3_35n9 encodes a novel 607 amino acid protein which is a splice variant of human carboxylesterase (EC 3.1.1.1).

The novel protein contains both, one carboxylesterase Bl and one B2 pattern. In comparison to EC 3.1.1.1, DKFZphtes3_35n9 shows a N-termmal extension and aa 458-474 are missing.

The new protein can find application m modulation of carboxylester metabolism and as a new enzyme for biotechnologic production processes. carboxylesterase, splice variant

5' extension of mRNA and N-terminal elongation of protein (64 aa) , missing exon¹ aa 458-474 of JC5408 are missing

Sequenced by DKFZ

Locus : unknown

Insert length: 2888 bp

Poly A stretch at pos. 2878, no polyadenylation signal found

1 CTCGGCCTGA GGTGCGAGAG AAGCGGTGAC CGCGGCCCTG GCTGCTCGGA

51 CCCGGGAACA TGATGGTCGC TGGAGCAGAA GGCGCTGAGA AGGGACCACG

101 GCGGCGCTGG GTCGTGCGAG CCAGTAGCGG GCTGAAACGT AGAGGCCAGA

151 ACCAGGTCTC AGGGGGCACT AAAGGCGGTC GGAGGTAATC CCCACACCGC

201 TTCCTCCTGG AAGTCAGGCT GGCCGGGAGC TCCCGTATCC AGGACGGTTG

251 GTCGCCTCTG GCCTGGCAGG GATCCTAGTG TCTCGGGACC TCCCGGTGAC

301 GCGCCTGCCT CCCCTGCTGC ACCATAGGCC CGGGAGTACG GCGTCCCCAC

351 AGCTTGGACC GGCAGGGGCT CGTGAAATGT TTGTCAAGTG GATAAATGAC

401 CATGGCCGTG GTCTCCGCGG GAGGTGAGGA AACTGAAAGC CACCGAGGAA

451 AAGGGGGGCG CTCCTTAAGA AGTGCCGCGG TCACGTGTAC GTTTCAAAAG

501 AATGGCGTGA CTGAGTAGGG AGGGGACCGC GGAGACCCTC AGACCCTGGA

551 CTGTAAGGAG ATGAGGGGCC GTGAAGGGGA ACCCAGGAAA CTGAGTCCTG

601 AAAGCAAGGA GGAACTTCCA GAATGAAGGG CGCCGACACT CCTTCCTGCC

651 TTTGCTCAAG CGGTTCCTTC ACCCCGATCA AGTTCCTTCC CATTTCTCCA

701 TCTGGGGGAT CCTGAACGTG CACATCCTCA GAGAAGCCCT CCTGGGGTCT

751 CCAATTCTAG TTTATTGCCC CCTCCTATCG ATCCCCCAGC GCGCTCATCG

801 GGCCTGTGGA CAAGGACAGG TTTGAAGAGA GGATTCCCTG GATCGCGGAA

851 GGGCTGCAGG AATGGCACAG CCCCTTCCGA GGATGCCAAA GGAGCCCGGG

901 CAAAGGAAAG TGGCCGTGCC CGGGCCTGCC TACCACTAGA TCCCCACCCA

951 CCTATGACTG CTCAGTCCCG CTCTCCTACC ACACCCACCT TTCCCGGCCC

1001 AAGCCAGCGC ACCCCGCTGA CTCCCTGCCC AGTCCAAACT CCAAGGCTGG

1051 GCAAGGCACT GATCCACTGC TGGACAGACC CGGGGCAGCC TCTGGGTGAA

1101 CAGCAGCGTG TCCGCCGGCA GCGAACCGAG ACCAGCGAGC CGACCATGCG

1151 GCTGCACAGA CTTCGTGCGC GGCTGAGCGC GGTGGCCTGT GGGCTTCTGC

1201 TGCTTCTTGT CCGGGGCCAG GGCCAGGACT CAGCCAGTCC CATCCGGACC

1251 ACACACACGG GGCAGGTGCT GGGGAGTCTT GTCCATGTGA AGGGCGCCAA

1301 TGCCGGGGTC CAAACCTTCC TGGGAATTCC ATTTGCCAAG CCACCTCTAG

1351 GTCCGCTGCG ATTTGCACCC CCTGAGCCCC CTGAATCTTG GAGTGGTGTG

1401 AGGGATGGAA CCACCCATCC GGCCATGTGT CTACAGGACC TCACCGCAGT

1451 GGAGTCAGAG TTTCTTAGCC AGTTCAACAT GACCTTCCCT TCCGACTCCA

1501 TGTCTGAGGA CTGCCTGTAC CTCAGCATCT ACACGCCGGC CCATAGCCAT

1551 GAAGGCTCTA ACCTGCCGGT GATGGTGTGG ATCCACGGTG GTGCGCTTGT

1601 TTTTGGCATG GCTTCCTTGT ATGATGGTTC CATGCTGGCT GCCTTGGAGA

1651 ACGTGGTGGT GGTCATCATC CAGTACCGCC TGGGTGTCCT GGGCTTCTTC

1701 AGCACTGGAG ACAAGCACGC AACCGGCAAC TGGGGCTACC TGGACCAAGT

1751 GGCTGCACTA CGCTGGGTCC AGCAGAATAT CGCCCACTTT GGAGGCAACC

1801 CTGACCGTGT CACCATTTTT GGCGAGTCTG CGGGTGGCAC GAGTGTGTCT

1851 TCGCTTGTTG TGTCCCCCAT ATCCCAAGGA CTCTTCCACG GAGCCATCAT

1901 GGAGAGTGGC GTGGCCCTCC TGCCCGGCCT CATTGCCAGC TCAGCTGATG

1951 TCATCTCCAC GGTGGTGGCC AACCTGTCTG CCTGTGACCA AGTTGACTCT

2001 GAGGCCCTGG TGGGCTGCCT GCGGGGCAAG AGTAAAGAGG AGATTCTTGC

2051 AATTAACAAG CCTTTCAAGA TGATCCCCGG AGTGGTGGAT GGGGTCTTCC

2101 TGCCCAGGCA CCCCCAGGAG CTGCTGGCCT CTGCCGACTT TCAGCCTGTC

2151 CCTAGCATTG TTGGTGTCAA CAACAATGAA TTCGGCTGGC TCATCCCCAA

2201 GGTCATGAGG ATCTATGATA CCCAGAAGGA AATGGACAGA GAGGCCTCCC

2251 AGGCTGCTCT GCAGAAAATG TTAACGCTGC TGATGTTGCC TCCTACATTT

2301 GGTGACCTGC TGAGGGAGGA GTACATTGGG GACAATGGGG ATCCCCAGAC

2351 CCTCCAAGCG CAGTTCCAGG AGATGATGGC GGACTCCATG TTTGTGATCC

2401 CTGCACTCCA AGTAGCACAT TTTCAGTGTT CCCGGGCCCC TGTGTACTTC

2451 TACGAGTTCC AGCATCAGCC CAGCTGGCTC AAGAACATCA GGCCACCGCA

2501 CATGAAGGCA GACCATGTTA AATTCACTGA GGAAGAGGAG CAGCTAAGCA

2551 GGAAGATGAT GAAGTACTGG GCCAACTTTG CGAGAAATGG GAACCCCAAT

2601 GGCGAGGGTC TGCCACACTG GCCGCTGTTC GACCAGGAGG AGCAATACCT 2651 GCAGCTGAAC CTACAGCCTG CGGTGGGCCG GGCTCTGAAG GCCCACAGGC 2701 TCCAGTTCTG GAAGAAGGCG CTGCCCCAAA AGATCCAGGA GCTCGAGGAG 2751 CCTGAAGAGA GACACACAGA GCTGTAGCTC CCTGTGCCGG GGAGGAGGGG 2801 GTGGGTTCGC TGACAGGCGA GGGTCAGCCT GCTGTGCCCA CACACACCCA 2851 CTAAGGAGAA AGAAGTTGAT TCCTTCATAA AAAAAAAA

BLAST Results

Entry D50579 from database EMBL:

Homo sapiens mRNA for carboxylesterase, complete eds.

Score = 7197, P = O.Oe+00, identities = 1441/1443

Entry JC5408 from database PIR: carboxylesterase (EC 3.1.1.1) - human

Score = 2808, P = 1.2e-291, identities = 542/559, positives = 543/559, frame +3

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 954 bp to 2774 bp; peptide length: 607

Category: known protein

Classification: Metabolism

Prosite motifs: CARBOXYLESTERASE_B_l (279-295)

CARBOXYLESTERASE B 2 (185-196)

1 MTAQSRΞPTT PTFPGPSQRT PLTPCPVQTP RLGKALIHCW TDPGQPLGEQ 51 QRVRRQRTET SEPTMRLHRL RARLSAVACG LLLLLVRGQG QDSASPIRTT 101 HTGQVLGSLV HVKGANAGVQ TFLGIPFAKP PLGPLRFAPP EPPESWSGVR 151 DGTTHPAMCL QDLTAVESEF LSQFNMTFPS DSMSEDCLYL SIYTPAHSHE 201 GSNLPVMVWI HGGALVFGMA SLYDGSMLAA LENVVVVIIQ YRLGVLGFFS 251 TGDKHATGNW GYLDQVAALR WVQQNIAHFG GNPDRVTIFG ESAGGTSVSS 301 LVVSPISQGL FHGAIMESGV ALLPGLIASS ADVISTVVAN LSACDQVDSE 351 ALVGCLRGKS KEEILAINKP FKMIPGVVDG VFLPRHPQEL LAΞADFQPVP 401 SIVGVNNNEF GWLIPKVMRI YDTQKEMDRE ASQAALQKML TLLMLPPTFG 451 DLLREEYIGD NGDPQTLQAQ FQEMMADSMF VIPALQVAHF QCSRAPVYFY 501 EFQHQPSWLK NIRPPHMKAD HVKFTEEEEQ LSRKMMKYWA NFARNGNPNG 551 EGLPHWPLFD QEEQYLQLNL QPAVGRALKA HRLQFWKKAL PQKIQELEEP 601 EERHTEL

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_35n9, frame 3

PIR:JC5408 carboxylesterase (EC 3.1.1.1) - human, N = 1, Score = 2808, P = 1.9e-292

TREMBL :HSU60553_1 gene: "hCE-2"; product: "carboxylesterase"; Human carboxylesterase (hCE-2) mRNA, complete eds., N = 1, Score = 2761, P = 1.8e-287

PIR:A34329 60K esterase (EC 3.1.1.-) isoform 2 - rabbit, N = 1, Score = 1985, P = 3.1e-205

TREMBL :D50580_1 product: "carboxylesterase precursor"; Rattus norvegicus mRNA for carboxylesterase, partial eds., N = 1, Score = 1984, P = 4e-205

>PIR:JC5408 carboxylesterase (EC 3.1.1.1) - human Length = 559

HSPs:

Score = 2808 (421.3 bits), Expect = 1.9e-292, P = 1.9e-292 Identities ^:= 542/559 (96%), Positives = 543/559 (97%)

Query: 65 MRLHRLRARLSAVACGLLLLLVRGQGQDSASPIRTTHTGQVLGSLVHVKGANAGVQTFLG 124 MRLHRLRARLSAVACGLLLLLVRGQGQDSASPIRTTHTGQVLGSLVHVKGANAGVQTFLG

Sbjct: 1 MRLHRLRARLSAVACGLLLLLVRGQGQDSASPIRTTHTGQVLGΞLVHVKGANAGVQTFLG 60

Query: 125 IPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPSDSMS 184 IPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPΞDSMS

Sbjct: 61 IPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPSDSMS 120

Query: 185 EDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLG 244 EDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLG

Sbjct: 121 EDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLG 180

Query: 245 VLGFFΞTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGEΞAGGTSVSSLVVS 304 VLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVVS

Sbjct: 181 VLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVVS 240

Query: 305 PIΞQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEI 364 PISQGLFHGAIMESGVALLPGLIAΞSADVISTVVANLΞACDQVDSEALVGCLRGKSKEEI

Sbjct: 241 PISQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEI 300

Query: 365 LAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQ 424 LAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQ

Sbjct: 301 LAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQ 360

Query: 425 KEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPA 484 KEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPA

Sbjct: 361 KEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPA 420

Query: 485 LQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADH VKFTEEE 528

LQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADH +KFTEEE

Sbjct : 421 LQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEE 480

Query: 529 EQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKK 588 EQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKK

Sbjct: 481 EQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKK 540

Query: 589 ALPQKIQELEEPEERHTEL 607 ALPQKIQELEEPEERHTEL

Sbjct: 541 ALPQKIQELEEPEERHTEL 559

Pedant information for DKFZphtes3_35n9, frame 3

Report for DKFZphtes3_35n9.3

(LENGTH] 607

[MW] 67051.20

[pi] 6.11

[HOMOL] PIR:JC5408 carboxylesterase (EC 3.1.1.1) - human 0.0

[BLOCKS] BL01173A Lipolytic enzymes G-D-X-G" family, histidine

[BLOCKS] BL00122G

[BLOCKS] BL00122F

[BLOCKS] BL00122E

[BLOCKS] BL00122D Carboxylesterases type-B serine proteins

[BLOCKS] BL00122C Carboxylesterases type-B serine proteins

[BLOCKS] BL00122B Carboxylesterases type-B serine proteins

[BLOCKS] BL00122A Carboxylesterases type-B serine proteins

[SCOP] dlakn 3.56.1.1.4 Bile-salt activated lipase [Bovine (Bos taurus le-156

[SCOP] d2ack 3.56.1.1.1 Acetylcholinesterase [Electric ray (Torped le-170

[SCOP] dlthg 3.56.1.9.7 type-B carboxylesterase/lipase [fungu le-149

[EC] 3.1.1.13 Sterol esterase le-52

[EC] 3.1.1.7 Acetylcholinesterase 5e-74

[EC] 3.1.1.1 Carboxylesterase 0.0

[EC] 3.1.1.8 Cholinesterase 5e-68

[EC] 3.1.1.59 Juvenile-hormone esterase le-34

[EC] 3.1.1.3 Triacylglycerol lipase 3e-52

[PIRKW] duplication 2e-47

[PIRKW] homotetramer 3e-67

[PIRKW] transmembrane protein 9e-44

[PIRKW] microsome le-130

[PIRKW] pancreas 3e-52

[PIRKW] endoplasmic reticulum le-134

[PIRKW] homotπmer le-134

[PIRKW] phosphatidylinositol linkage 5e-74

[PIRKW] synapse 3e-73

[PIRKW] liver le-131

[PIRKW] heparm binding 3e-52 [PIRKW] phosphoprotein 7e-25

[PIRKW] glycoprotein le-134

[PIRKW] thyroid hormone biosynthesis 2e-47

[PIRKW] carboxylic ester hydrolase 0.0

[PIRKW] monomer 2e-42

[PIRKW] disulfide bond 2e-31

[PIRKW] mammary gland 3e-52

[PIRKW] alternative splicing 5e-74

[PIRKW] iodine 2e-47

[PIRKW] pyroglutamic ac d 6e-39

[PIRKW] hydrolase le-135

[PIRKW] muscle 3e-73

[PIRKW] thyroid gland 2e-47

[PIRKW] membrane protein 3e-73

[PIRKW] neurotransmitter degradation 3e-73

[PIRKW] cholesterol 3e-52

[PIRKW] homodimer 2e-47

[PIRKW] nerve 3e-73

[SUPFAM] cholinesterase 0.0

[SUPFAM] triacylglycerol lipase le-32

[SUPFAM] cholinesterase homology 0.0

[SUPFAM] thyroglobulin 2e-47

[SUPFAM] thyroglobulin type I repeat homology 2e-47

[SUPFAM] juvenile-hormone esterase 2e-35

[SUPFAM] probable lipolytic protein ybaC le-07

[PROSITE] CARBOXYLESTERASE_B_2 1

[PROSITE] CARBOXYLESTERASE_B_l 1

[PFAM] Carboxylesterases

[KW] Alpha_Beta

[KW] 3D

[KW] LOW COMPLEXITY 3.95 %

SEQ MTAQSRSPTTPTFPGPSQRTPLTPCPVQTPRLGKALIHCWTDPGQPLGEQQRVRRQRTET SEG xxxxxxx ... lacj-

SEQ SEPTMRLHRLRARLSAVACGLLLLLVRGQGQDSASPIRTTHTGQVLGSLVHVKGANAGVQ SEG xxxxx lacj- ETTEEEECEEEEETTEE—EE

SEQ TFLGIPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPS SEG lacj- EEEEEECEETTTGGGTTTCCEECCCCCCEEECCCCCCBCCCCCCTTTTTT-HHHHHCCCC

SEQ DSMSEDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQ SEG lacj- CCBTTTTCEEEEEET—TTTTTTEEEEEEECTTTTTTCTTTTGCHHHHHHHHCCEEEECC

ΞEQ YRLGVLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSS SEG lacj- CCCCGGGCCCTTTTTTTCCHHHHHHHHHHHHHHHCGGGGCEEEEEEEEEEECHHHHHHHH

SEQ LVVSPISQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKS SEG lacj- HHHCGGGTTTTCEEEEETTTTTTTTTTBCHHHHHHHHHHHHC-CCCCCHHHHHHHHHHCC

SEQ KEEILAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRI SEG lacj- HHHHHHHHTCCCTTTCBTTTTTTTTTHHHHHHHTTTCCCCEEEEEETBTHHHHHHTTTTT

SEQ YDTQKEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMF SEG lacj- TTTCCCCCHHHHHHHHHHHTTTTCHHHHHHHHHHCTTTTTTTHHHH-HHHHHHHHHHHHH

SEQ VIPALQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHVKFTEEEEQLSRKMMKYWA SEG lacj- HHHHHHHHHHHHCCCCEEEEEECCCCGGGTTBTTTHHHCGGGCCCHHHHHHHHHHHHHHH

SEQ NFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEP SEG xxxxx lacj- HHHHHCCCCCCC—CCCCBTTTTBEEEECCCCCEEETTTHHHHHHHHHHHHH

SEQ EERHTEL SEG xxxxxx .

Prosite for DKFZphtes3 35n9.3 PS00122 279->295 CARBOXYLESTERAΞE_B_l PDOC00112 PS00941 185->196 CARBOXYLESTERASE_B_2 PDOC00112

Pfam for DKFZphtes3_35n9.3

HMM_NAME Carboxylesterases

HMM *MfMnwlιmFLLwmItWIι .WheqaprpPdPyiVdtnnCGklRGmNedtD

+ +L+++ ++++++++ ++Q++++P I T+ G + G ++ + Query 69 RLRARLSAVACGLLLLLVRGQGQDΞASP IRTTHT-GQVLGSLVHVK 113

HMM NG..pYYvFlGIPYAEPPVGNLRFKePQPYhePWtNVWNATnYPPMCMQW

+ + +FLGIP+A+PP+G LRF +P+P +E W++V++ T+ P MC+Q+ Query 114 GANAGVQTFLGIPFAKPPLGPLRFAPPEP-PESWSGVRDGTTHPAMCLQD 162

HMM ndFGFWlFdmieMWNemP.. eMSEDCLYLNVWTPWnrkPNskLPVMVWI

+++ +++N++ P +MSEDCLYL+++TP+ + ++S+LPVMVWI Query 163 LTAV—ESEFLSQFNMTFPSDSMSEDCLYLSIYTPAHSHEGSNLPVMVWI 210

HMM HGGGFMFGSGhsYPliqYDgeylMMeeNVIVVtlNYRLGPFGFLSTgDid HGG+++FG + ++YDG+ L++ ENV+VV I+YRLG++GF+STGD + Query 211 HGGALVFGMA SLYDGSMLAALENVVVVIIQYRLGVLGFFSTGDKH 255

HMM lPPHGNWGLWDQRMALQWVQDNIAnFGGDPNNITIFGESAGGMSVHIHML

+ GNWG++DQ++AL+WVQ+NIA+FGG+P+++TIFGESAGG+SV+ ++ Query 256 AT—GNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVV 303

HMM SYGGDNPPmfKqLFHRAIMQSGsAmcPWvIQsnyNaRqRAfRFAπmGCN S P + +LFH AIM+SG A+ P++I S++ + +A++ C+ Query 304 Ξ PISQGLFHGAIMESGVALLPGLIASSA—DVISTVVANLSACD 345

HMM rmDssEMIqCLRsKPwEELWdAtWnFWmWfYfPFlPWFFgPVIDGDDaPE

+ DS++++ CLR K+ EE++++++ +F + + +DG+ Query 346 QVDSEALVGCLRGKSKEEILAINK PFKMIPGV VDGV 381

HMM aFIPDHPeeMIkEGkFnDVPWIIGYNnDEGiWFapMmMnfnWfdEDeWId

F+P+HP+E++++ F VP I+G+NN E++W++P M + + +E++ Query 382 -FLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDT-QKEMDR 429

HMM ltNedWyeWMPYHFYrddmsNikDMDDYiDkvyEeYPgWWDrFPqESYW

++ + ++ M +L + + + D ++EEY+G+ + PQ

Query 430 EASQAALQKMLTLLMLPPT-F GDLLREEYIGDNGD-PQTLQA 469

HMM nLqDMFTDYLFWCPtRihadnHRkHwgsPVYMYeFDHPpSFGYgQFFmWR

++Q+M+ D F++P + ++H++ +PVY+YEF+H PS + Query 470 QFQEMMADΞMFVIP—ALQVAHFQCSRAPVYFYEFQHQPSW LKN 511

HMM WWPpWMgvdH*

+PP+M++DH Query 512 IRPPHMKADH 521

HMM *tEEEnssMRmMMNYWINFAKhGNPNnthnglCWWPqYTsnEQYdMIMe

TEEE+ +S R MM+YW+NFA++GNPN++ GL++WP ++++EQY++ + Query 525 TEEEEQLS-RKMMKYWANFARNGNPNGE—GLPHWPLFDQEEQYLQLNL 570

HMM tllmiQmCrmrDPYCNFW*

+ +++++ + FW Query 571 QPAVGRALKAHR—LQFW 586

DKFZphtes3_35pl7

group: testes derived

DKFZphtes3_35pl7 encodes a novel 505 ammo acid protein with weak similarity to Proteins of the armadillo family.

Proteins of the armadillo family are involved in diverse cellular processes in higher eukaryotes. Some of them, like armadillo, beta-catemn and plakoglobins have dual functions in intercellular junctions and signalling cascades. Others, belonging to the lmportin-alpha- subfamily are involved in NLS recognition and nuclear transport, while some members of the armadillo family have as yet unknown functions. The novel protein shows similarity to S. cerevisiae protein Yel013p (VAC8) and Danio rerio b-catenm, but contains no armadillo (arm) repeats . No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes. similarity to S. cerevisiae VAC8 complete cDNA, complete eds, few EST hits

Sequenced by DKFZ

Locus : unknown

Insert length: 1966 bp

Poly A stretch at pos. 1956, polyadenylation signal at pos. 1935

1 AAGTCAAATG TAAGATTGGT TCATTAAAAA TACTGAAGGA AATCAGTCAT

51 AATCCTCAAA TCAGACAGAA TATTGTTGAC CTTGGGGGCT TACCAATTAT

101 GGTGAATATA CTTGATTCTC CACACAAGAG TCTAAAATGT TTGGCAGCCG

151 AGACTATCGC GAATGTTGCC AAGTTTAAAA GAGCACGGCG GGTGGTGAGG

201 CAGCACGGGG GTATCACCAA ACTGGTTGCT CTACTAGACT GTGCACATGA

251 TTCCACAAAA CCTGCCCAAT CGAGTCTGTA TGAGGCCAGA GACGTGGAAG

301 TGGCTCGCTG TGGGGCACTG GCCCTGTGGA GCTGCAGTAA GAGTCATACG

351 AATAAAGAAG CCATCCGCAA AGCTGGGGGC ATTCCTCTGT TGGCTCGGCT

401 GCTGAAGACT TCTCATGAAA ACATGCTAAT TCCAGTGGTG GGGACATTGC

451 AAGAGTGTGC ATCAGAGGAA AACTACCGGG CTGCAATCAA AGCAGAAAGG

501 ATCATTGAAA ACCTTGTCAA GAACCTAAAT AGTGAGAATG AGCAGCTGCA

551 GGAGCACTGC GCCATGGCCA TTTACCAGTG TGCTGAAGAT AAGGAAACCC

601 GGGACCTCGT TAGGCTGCAC GGAGGACTTA AGCCCTTGGC CAGTCTACTC

651 AATAACACTG ACAATAAAGA GCGGTTAGCT GCTGTCACAG GGGCTATATG

701 GAAATGTTCC ATCAGCAAAG AGAATGTTAC CAAGTTTCGG GAATACAAAG

751 CCATTGAAAC CTTGGTGGGA CTTCTAACAG ATCAGCCTGA AGAAGTACTT

801 GTGAATGTGG TTGGGGCCTT GGGAGAATGC TGCCAAGAAC GTGAAAACCG

851 AGTCATTGTC CGGAAATGTG GTGGCATTCA ACCACTTGTG AACCTCCTTG

901 TTGGAATAAA CCAAGCTCTT CTTGTGAATG TTACAAAAGC AGTTGGTGCT

951 TGTGCAGTAG AACCTGAAAG TATGATGATA ATTGATCGCT TAGATGGAGT

1001 TCGTTTGTTG TGGTCCCTGC TGAAAAATCC TCACCCAGAC GTGAAGGCCA

1051 GCGCAGCATG GGCACTCTGT CCATGCATCA AAAATGCAAA GGATGCTGGG

1101 GAAATGGTTC GTTCCTTTGT TGGTGGTTTG GAACTTATTG TCAATTTACT

1151 GAAATCAGAT AACAAAGAAG TTCTGGCAAG TGTATGTGCT GCCATTACCA

1201 ACATAGCAAA AGATCAAGAA AATTTAGCTG TTATCACAGA TCATGGAGTT

1251 GTTCCTTTAT TGTCCAAACT GGCAAATACA AATAACAATA AATTGAGACA

1301 TCATCTAGCA GAAGCTATTT CACGTTGCTG TATGTGGGGC AGGAATAGAG

1351 TGGCCTTCGG TGAGCACAAA GCAGTGGCTC CACTAGTGCG TTATCTGAAA

1401 TCAAATGACA CCAACGTGCA TCGGGCGACA GCTCAGGCCT TGTACCAACT

1451 CTCAGAAGAC GCCGATAACT GCATCACCAT GCATGAGAAT GGTGCAGTAA

1501 AGCTTCTACT GGATATGGTT GGGTCCCCTG ACCAGGATCT CCAGGAAGCT

1551 GCAGCTGGTT GTATATCCAA TATCCGCAGG CTGGCTCTTG CTACAGAGAA

1601 GGCAAGATAC ACTTGAAATT TAAATGGACA TTACAAGCTA TCAAATTCTA

1651 CATGACACAG GACATGTCAC TCCCATGGCC AGAAAGCCTA AATTGGGAAA

1701 CAGTTGTTAG CAAACCCTTT CAACCATCTA AATGAAAACA CACAAATTGA

1751 AAATGCACAG AATGTTTTTC ATCTGAAAAT TGCATGGAGA CTTTTGTTTC

1801 TATTTAATGT TTTCGAGATA TGACATGTGA TAAGATGGAA AGCCAATAAA

1851 CCTGTGATAA GTTTCTAAGA ATATGAGAAT ATACGTATAT GATGTATTTT

1901 TAGTTCAGTG ATGCTTTTGT ATTTGTGGCG ATTTTAATAA AGGATATGGC

1951 CTTCCCAAAA AAAAAA

BLAST Results

o BLAST result Medlme entries

98413148:

Yel013p (Vac8p) , an armadillo repeat protein related to plakoglobm and importin alpha is associated with the yeast vacuole membrane.

98330438:

YEB3/VAC8 encodes a myristylated armadillo protein of the Saccharomyces cerevisiae vacuolar membrane that functions in vacuole fusion and inheritance.

98158703:

Vac8p, a vacuolar protein with armadillo repeats, functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole .

Peptide information for frame 3

ORF from 99 bp to 1613 bp; peptide length: 505 Category: similarity to known protein Classification: unset

1 MVNILDSPHK SLKCLAAETI ANVAKFKRAR RVVRQHGGIT KLVALLDCAH

51 DSTKPAQSSL YEARDVEVAR CGALALWSCS KSHTNKEAIR KAGGIPLLAR

101 LLKTSHENML IPVVGTLQEC ASEENYRAAI KAERIIENLV KNLNSENEQL

151 QEHCAMAIYQ CAEDKETRDL VRLHGGLKPL ASLLNNTDNK ERLAAVTGAI

201 WKCSISKENV TKFREYKAIE TLVGLLTDQP EEVLVNVVGA LGECCQEREN

251 RVIVRKCGGI QPLVNLLVGI NQALLVNVTK AVGACAVEPE SMMIIDRLDG

301 VRLLWSLLKN PHPDVKASAA WALCPCIKNA KDAGEMVRSF VGGLELIVNL

351 LKSDNKEVLA SVCAAITNIA KDQENLAVIT DHGVVPLLSK LANTNNNKLR

401 HHLAEAISRC CMWGRNRVAF GEHKAVAPLV RYLKSNDTNV HRATAQALYQ

451 LSEDADNCIT MHENGAVKLL LDMVGSPDQD LQEAAAGCIS NIRRLALATE 501 KARYT

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_35pl7 , frame 3

PIR:S50446 VAC8 protein - yeast (Saccharomyces cerevisiae), N = 1, Score = 237, P = 7.8e-17

PIR:T00403 T13E15.9 protein - Arabidopsis thaliana, N = 1, Score = 215, P = 4.9e-14

TREMBL: DR41081_1 product: "b-catenm"; Danio rerio b-catenin mRNA, complete eds., N = 1, Score = 195, P = 5.8e-12

>PIR:S50446 VAC8 protein - yeast (Saccharomyces cerevisiae) Length = 578

HSPs:

Score = 237 (35.6 bits), Expect = 7.8e-17, P = 7.8e-17 Identities = 106/401 (26%), Positives = 177/401 (44%)

Query: 92 AGGIPLLARLLKTSHENMLIPVVGTLQECASEENYRAAIKAERIIENLVKNLNSENEQLQ 151

+GG PL A +N+ + L E Y + E ++E ++ L S++ Q+Q Sbjct: 45 SGG-PLKALTTLVYSDNLNLQRSAALAFAEITEKYVRQVSRE-VLEPILILLQSQDPQIQ 102

Query: 152 EHCAMAIYQCAEDKETRDLVRLHGGLKPLASLLNNTDNKERLAAVTGAIWKCSISKENVT 211

A+ A + E + L+ GGL+PL + + DN E G I + +N Sbjct: 103 VAACAALGNLAVNNENKLLIVEMGGLEPLINQMMG-DNVEVQCNAVGCITNLATRDDNKH 161

Query: 212 KFREYKAIETLVGLLTDQPEEVLVNVVGALGECCQERENRVIVRKCGGIQPLVNLLVGIN 271

K A+ L L + V N GAL ENR + G + LV+LL + Sbjct: 162 KIATSGALIPLTKLAKSKHIRVQRNATGALLNMTHSEENRKELVNAGAVPVLVSLLSSTD 221

Query: 272 QALLVNVTKAVGACAVEPESMMIIDRLDG—VRLLWSLLKNPHPDVKASAAWALCPCIKN 329 + T A+ AV+ + + + + V L SL+ +P VK A AL + Sbjct: 222 PDVQYYCTTALSNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRVKCQATLALRNLASD 281

Query: 330 AKDAGEMVRSFVGGLELIVNLLKSDNKE-VLASVCAAITNIAKDQENLAVITDHG V-PL 387

E+VR+ GGL +V L++SD+ VLAΞV A I NI+ N +1 D G + PL Sbjct: 282 TSYQLEIVRA—GGLPHLVKLIQSDSIPLVLASV-ACIRNISIHPLNEGLIVDAGFLKPL 338

Query: 388 LSKLANTNNNKLRHHLAEAISRCCMWG-RNRVAFGEHKAVAPLVRYLKSNDTNVHRATAQ 446

+ L ++ +++ H + +NR F E AV + +V ++

Sbjct: 339 VRLLDYKDSEEIQCHAVSTLRNLAASSEKNRKEFFEΞGAVEKCKELALDSPVSV-QΞEIS 397

Query: 447 ALYQLSEDAD-NCITMHENGAVKLLLDMVGSPDQDLQEAAAGCISNI 492

A + + AD + + + E + L+ M S +Q++ AA ++N+ Sbjct: 398 ACFAILALADVSKLDLLEANILDALIPMTFSQNQEVSGNAAAALANL 444

Score = 213 (32.0 bits), Expect = 3.6e-14, P = 3.6e-14 Identities = 81/341 (23%), Positives = 163/341 (47%)

Query: 163 EDKETRDLVRLHGGLKPLASLLNNTD-NKERLAAVTGAIWKCSISKENVTKFREYKAIET 221

EDK+ D G LK L +L+ + + N +R AA+ A I+++ V + + +E Sbjct: 36 EDKDQLDFYS-GGPLKALTTLVYSDNLNLQRSAALAFA EITEKYVRQVSR-EVLEP 89

Query: 222 LVGLLTDQPEEVLVNVVGALGECCQERENRVIVRKCGGIQPLVNLLVGINQALLVNVTKA 281

++ LL Q ++ V ALG EN++++ + GG++PL+N ++G N + N Sbjct: 90 ILILLQSQDPQIQVAACAALGNLAVNNENKLLIVEMGGLEPLINQMMGDNVEVQCNAVGC 149

Query: 282 VGACAVEPESMMIIDRLDGVRLLWSLLKNPHPDVKASAAWALCPCIKNAKDAGEMVRSFV 341

+ A ++ I + L L K+ H V+ +A AL + ++ E+V + Sbjct: 150 ITNLATRDDNKHKIATSGALIPLTKLAKΞKHIRVQRNATGALLNMTHSEENRKELVNA— 207

Query: 342 GGLELIVNLLKSDNKEVLASVCAAITNIAKDQENLAVI—TDHGVVPLLSKLANTNNNKL 399

G + ++V+LL S + +V A++NIA D+ N + T+ +V L L ++ ++++ Sbjct: 208 GAVPVLVSLLSSTDPDVQYYCTTALSNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSΞRV 267

Query: 400 RHHLAEAISRCCMWGRNRVAFGEHKAVAPLVRYLKSNDTNVHRATAQALYQLSEDADNCI 459

+ A+ ++ + LV+ ++S+ + A+ + +S N

Sbjct: 268 KCQATLALRNLASDTSYQLEIVRAGGLPHLVKLIQSDSIPLVLASVACIRNISIHPLNEG 327

Query: 460 TMHENGAVKLLLDMVGSPDQDLQEAAAGCISNIRRLALATEKAR 503

+ + G +K L+ ++ D + E +S +R LA ++EK R Sbjct: 328 LIVDAGFLKPLVRLLDYKDSE—EIQCHAVSTLRNLAAΞSEKNR 369

Score = 180 (27.0 bits), Expect = 1.6e-10, P = 1.6e-10 Identities = 80/346 (23%), Positives = 142/346 (41%)

Query: 145 SENEQLQEHCAMAIYQCAEDKETRDLVRLHGGLKPLASLLNNTDNKERLAAVTGAIWKCΞ 204

S+N LQ A+A + E K R + R L+P+ LL + D + ++AA A+ + Sbjct: 58 SDNLNLQRSAALAFAEITE-KYVRQVSR—EVLEPILILLQSQDPQIQVAACA-ALGNLA 113

Query: 205 ISKENVTKFREYKAIETLVGLLTDQPEEVLVNVVGALGECCQERENRVIVRKCGGIQPLV 264

++ EN E +E L+ + EV N VG + +N+ + G + PL Sbjct: 114 VNNENKLLIVEMGGLEPLINQMMGDNVEVQCNAVGCITNLATRDDNKHKIATSGALIPLT 173

Query: 265 NLLVGINQALLVNVTKAVGACAVEPESMMIIDRLDGVRLLWSLLKNPHPDVKASAAWALC 324

L + + N T A+ E+ + V +L SLL + PDV+ AL Sbjct: 174 KLAKSKHIRVQRNATGALLNMTHΞEENRKELVNAGAVPVLVSLLSSTDPDVQYYCTTALS 233

Query: 325 PCIKNAKDAGEMVRSFVGGLELIVNLLKSDNKEVLASVCAAITNIAKDQENLAVITDHGV 384

+ + ++ ++ + +V+L+ S + V A+ N+A D I G Sbjct: 234 NIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRVKCQATLALRNLASDTΞYQLEIVRAGG 293

Query: 385 VPLLSKLANTNNNKLRHHLAEAISRCCMWGRNRVAFGEHKAVAPLVRYLKΞNDTNVHRAT 444

+P L KL +++ L I + N + + PLVR L D+ + Sbjct: 294 LPHLVKLIQSDSIPLVLASVACIRNISIHPLNEGLIVDAGFLKPLVRLLDYKDSEEIQCH 353

Query: 445 A-QALYQLSEDAD-NCITMHENGAVKLLLDMVGSPDQDLQEAAAGCIS 490

A L L+ ++ N E+GAV+ ++ +Q + C + Sbjct: 354 AVSTLRNLAASSEKNRKEFFESGAVEKCKELALDSPVSVQSEISACFA 401

Score = 155 (23.3 bits), Expect = 8.8e-08, P = 8.8e-08 Identities = 88/401 (21%), Positives = 175/401 (43%)

Query: 60 LYEARD—VEVARCGALALWSCSKSHTNKEAIRKAGGI-PLLARLLKTSHENMLIPVVGT 116

L +++D ++VA C AL + + ++ NK I + GG+ PL+ +++ + E + VG Sbjct: 93 LLQSQDPQIQVAACAALG—NLAVNNENKLLIVEMGGLEPLINQMMGDNVE-VQCNAVGC 149

Query: 117 LQECASEENYRAAIKAERIIENLVKNLNSENEQLQEHCAMAIYQCAEDKETR-DLVRLHG 175

+ A+ ++ + I + L K S++ ++Q + A+ +E R +LV G Sbjct: 150 ITNLATRDDNKHKIATΞGALIPLTKLAKSKHIRVQRNATGALLNMTHSEENRKELVNA-G 208

Query: 176 GLKPLASLLNNTDNKERLAAVTGAIWKCSISKENVTKFR—EYKAIETLVGLLTDQPEEV 233 + L SLL++TD + T A+ ++ + N K E + + LV L+ V Sbjct: 209 AVPVLVSLLΞSTDPDVQYYCTT-ALSNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRV 267

Query: 234 LVNVVGALGECCQERENRVIVRKCGGIQPLVNLLVGINQALLVNVTKAVGACAVEPESMM 293

AL + ++ + + GG+ LV L+ + L++ + ++ P + Sbjct: 268 KCQATLALRNLASDTSYQLEIVRAGGLPHLVKLIQSDSIPLVLASVACIRNISIHPLNEG 327

Query: 294 IIDRLDGVRLLWSLLK-NPHPDVKASAAWALCPCIKNA-KDAGEMVRSFVGGLELIVNLL 351

+1 ++ L LL +++ A L ++ K+ E S G +E L Sbjct: 328 LIVDAGFLKPLVRLLDYKDΞEEIQCHAVΞTLRNLAASSEKNRKEFFES—GAVEKCKELA 385

Query: 352 KSDNKEVLA—SVCAAITNIAKDQENLAVITDHGVVPLLSKLANTNNNKLRHHLAEAISR 409

V + S C AI +A D L ++ + ++ L + + N ++ + A A++ Sbjct: 386 LDΞPVSVQSEISACFAILALA-DVSKLDLL-EANILDALIPMTFSQNQEVSGNAAAALAN 443

Query: 410 CCMWGRNRVAFGE HKAVAP-LVRYLKSNDTNVHRATAQALYQLSE 453

C N E ++ + L+R+LKS+ + QL E

Sbjct: 444 LCSRVNNYTKIIEAWDRPNEGIRGFLIRFLKSDYATFEHIALWTILQLLE 493

Score = 139 (20.9 bits), Expect = 5.0e-06, P = 5.0e-06 Identities = 80/329 (24%), Positives = 142/329 (43%)

Query: 37 GGITKLVALLDCAHD-STKPAQ SSLYEARDVEVARCGALALWSCSKSHTNKEAIRKA 92

G IT L D H +T A + L +++ + V R AL + + S N++ + A Sbjct: 148 GCITNLATRDDNKHKIATSGALIPLTKLAKΞKHIRVQRNATGALLNMTHSEENRKELVNA 207

Query: 93 GGIPLLARLLKTSHENMLIPVVGTLQECASEE-NYRAAIKAE-RIIENLVKNLNSENEQL 150

G +P+L LL ++ ++ L A +E N + + E R++ LV ++S + ++ Sbjct: 208 GAVPVLVSLLSSTDPDVQYYCTTALSNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRV 267

Query: 151 QEHCAMAIYQCAEDKETR-DLVRLHGGLKPLASLLNNTDNKERLAAVTGAIWKCSISKEN 209

+ +A+ A D + ++VR GGL L L+ + D+ + A I SI N Sbjct: 268 KCQATLALRNLASDTSYQLEIVRA-GGLPHLVKLIQS-DSIPLVLASVACIRNISIHPLN 325

Query: 210 VTKFREYKAIETLVGLLT-DQPEEVLVNVVGALGECCQERE-NRVIVRKCGGIQPLVNLL 267

+ ++ LV LL EE+ + V L E NR + G ++ L Sbjct: 326 EGLIVDAGFLKPLVRLLDYKDSEEIQCHAVSTLRNLAASSEKNRKEFFESGAVEKCKELA 385

Query: 268 VG—INQALLVNVTKAVGACA-VEPESMMIIDRLDGVRLLWSLLKNPHPDVKASAAWA-L 323

+ ++ ++ A+ A A V ++ + LD + + + +N A+AA A L Sbjct: 386 LDSPVSVQSEISACFAILALADVSKLDLLEANILDAL-IPMTFSQNQEVSGNAAAALANL 444

Query: 324 CPCIKN-AKDAGEMVRSFVGGLELIVNLLKSD 354

C + N K R G ++ LKSD Sbjct: 445 CSRVNNYTKIIEAWDRPNEGIRGFLIRFLKSD 476

Score = 136 (20.4 bits), Expect = l.le-05, P = l.le-05 Identities = 72/304 (23%), Positives = 133/304 (43%)

Query: 58 SSLYEARDVEVARCGALALWSCΞKSHTNKEAIRKAGGIPLLARLLKTSHENMLIPVVGTL 117

+ L +++ + V R AL + + S N++ + AG +P+L LL ++ ++ L Sbjct: 173 TKLAKSKHIRVQRNATGALLNMTHSEENRKELVNAGAVPVLVSLLSSTDPDVQYYCTTAL 232

Query: 118 QECASEE-NYRAAIKAE-RIIENLVKNLNSENEQLQEHCAMAIYQCAEDKETR-DLVRLH 174

A +E N + + E R++ LV ++S + +++ +A+ A D + ++VR Sbjct: 233 SNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRVKCQATLALRNLASDTSYQLEIVRA- 291

Query: 175 GGLKPLASLLNNTDNKERLAAVTGAIWKCSISKENVTKFREYKAIETLVGLLT-DQPEEV 233

GGL L L+ + D+ + A I SI N + ++ LV LL EE+ Sbjct: 292 GGLPHLVKLIQS-DSIPLVLASVACIRNISIHPLNEGLIVDAGFLKPLVRLLDYKDSEEI 350

Query: 234 LVNVVGALGECCQERE-NRVIVRKCGGIQPLVNLLVG—INQALLVNVTKAVGACA-VEP 289

+ V L E NR + G ++ L + ++ ++ A+ A A V Sbjct: 351 QCHAVSTLRNLAASSEKNRKEFFESGAVEKCKELALDSPVSVQSEISACFAILALADVSK 410

Query: 290 ESMMIIDRLDGVRLLWSLLKNPHPDVKAΞAAWA-LCPCIKN-AKDAGEMVRSFVGGLELI 347

++ + LD + + + +N A+AA A LC + N K R G + Sbjct: 411 LDLLEANILDAL-IPMTFSQNQEVSGNAAAALANLCSRVNNYTKIIEAWDRPNEGIRGFL 469

Query: 348 VNLLKSD 354

+ LKSD Sbjct: 470 IRFLKSD 476

Score = 114 (17.1 bits), Expect = 2.7e-03, P = 2.7e-03 Identities = 71/335 (21%), Positives = 132/335 (39%)

Query: 1 MVNILDSPHKSLKCLAAETIANVAKFKRARRVVRQHGGITKLVALLDCAHDSTKPAQSSL 60

+ + S H ++ A + N+ + R+ + G + LV+LL ST P Sbjct: 172 LTKLAKSKHIRVQRNATGALLNMTHSEENRKELVNAGAVPVLVSLLS STDP 222

Query: 61 YEARDVEVARCGALALWSCSKSHTNKEAIRKAGGIPLLARLLKTSHENMLIPVVGTLQEC 120 DV+ AL+ + +++ K A + + L L+ + + L+ Sbjct: 223 DVQYYCTTALSNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRVKCQATLALRNL 278

Query: 121 ASEENYRAAIKAERIIENLVKNLNSENEQLQEHCAMAIYQCAEDKETRDLVRLHGGLKPL 180 AS+ +Y+ I + +LVK + S++ L I + L+ G LKPL

Sbjct: 279 ASDTSYQLEIVRAGGLPHLVKLIQSDSIPLVLASVACIRNISIHPLNEGLIVDAGFLKPL 338

Query: 181 ASLLNNTDNKERLAAVTGAIWKCSISKE-NVTKFREYKAIETLVGLLTDQPEEVLVNVVG 239

LL+ D++E + + S E N +F E A+E L D P V +

Sbjct: 339 VRLLDYKDSEEIQCHAVSTLRNLAASSEKNRKEFFESGAVEKCKELALDSPVSVQSEISA 398

Query: 240 ALGECCQERENRVIVRKCGGIQPLVNLLVGINQALLVNVTKAVG-ACAVEPESMMIIDRL 298

+++ + + + L+ + NQ + N A+ C+ 11+

Sbjct: 399 CFAILALADVSKLDLLEANILDALIPMTFSQNQEVSGNAAAALANLCSRVNNYTKIIEAW 458

Query: 299 D GVR-LLWSLLKNPHPDVKASAAWALCPCIKNAKDAGE 335

D G+R L LK+ + + A W + +++ D E

Sbjct: 459 DRPNEGIRGFLIRFLKSDYATFEHIALWTILQLLESHNDKVE 500

Score = 106 (15.9 bits), Expect = 2.0e-02, P = 2.0e-02

Identitles ^:= 49/204 (24%), Positives = 89/204 (43%)

Query: 65 DVEVARCGALA-LWSCSKSHTNKEAIRKAGGIPLLARLLKTSHENMLIPVVGTLQECA-S 122 +VEV +C A+ + + + NK I +G + L +L K+ H + G L S

Sbjct: 139 NVEV-QCNAVGCITNLATRDDNKHKIATSGALIPLTKLAKSKHIRVQRNATGALLNMTHS 197

Query: 123 EENYRAAIKAERIIENLVKNLNΞENEQLQEHCAMAIYQCAEDKETRD-LVRLHGGL-KPL 180 EEN + + A + LV L+S + +Q +C A+ A D+ R L + L L

Sbjct: 198 EENRKELVNAGAV-PVLVSLLSSTDPDVQYYCTTALSNIAVDEANRKKLAQTEPRLVSKL 256

Query: 181 ASLLNNTDNKERLAAVTGAIWKCSISKENVTKFREYKAIETLVGLLTDQPEEVLVNVVGA 240

SL+++ ++ + A T A+ + + + LV L+ +++ V

Sbjct: 257 VSLMDSPSSRVKCQA-TLALRNLASDTSYQLEIVRAGGLPHLVKLIQSDSIPLVLASVAC 315

Query: 241 LGECCQERENRVIVRKCGGIQPLVNLL 267 + N ++ G ++PLV LL

Sbjct: 316 IRNISIHPLNEGLIVDAGFLKPLVRLL 342

Pedant information for DKFZphtes3_35pl7, frame 3

Report for DKFZphtes3_35pl7.3

[LENGTH] 505

[MW] 55224.34

[pi] 8.43

[HOMOL] PIR:S50446 VAC8 protein - yeast (Saccharomyces cerevisiae) 2e-16

[FUNCAT] 30.25 vacuolar and lysosomal organization [S. cerevisiae, YEL013w] 8e-18

[FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YEL013w]

8e-18

[FUNCAT] 09.25 vacuolar and lysosomal biogenesis [S. cerevisiae, YEL013w] 8e-18

[FUNCAT] 08.01 nuclear transport [S. cerevisiae, YNL189w] 3e-06

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YNL189w] 3e-06

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YNL189w] 3e-06

[BLOCKS] BL01265C

[BLOCKS] BL00242A Integrins alpha chain proteins

[SCOP] d3bct 1.91.1.1.1 beta-Catemn [Mouse (Mus musculus) 7e-18

[PIRKW] cytosol 3e-ll

[PIRKW] apoptosis 3e-ll

[PIRKW] carcinogenesis 3e-ll

[PIRKW] cell adhesion 3e-ll

[PIRKW] cytoskeleton 3e-12

[SUPFAM] pendulm le-07

[KW] All_Alpha

[KW] 3D

[KW] LOW COMPLEXITY 2.38 %

SEQ MVNILDSPHKSLKCLAAETIANVAKFKRARRVVRQHGGITKLVALLDCAHDSTKPAQSSL

SEG xxxxxxxxxxxx

2bct- HH

SEQ YEARDVEVARCGALALWSCSKSHTNKEAIRKAGGIPLLARLLKTSHENMLIPVVGTLQEC SEG 2bct- HHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHGGGCCCHHHHHHHHHHHHHH

SEQ ASEENYRAAIKAERIIENLVKNLNSENEQLQEHCAMAIYQCAEDKETRDLVRLHGGLKPL SEG 2bct- HHTTTHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHTTHHHHHHHHHHCHHHHH SEQ ASLLNNTDNKERLAAVTGAIWKCSISKENVTKFREYKAIETLVGLLTDQPEEVLVNVVGA

SEG

2bct- HHHHH-HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHTTTTTCCHHHHHHHHHH

SEQ LGECCQERENRVIVRKCGGIQPLVNLLVGINQALLVNVTKAVGACAVEPESMMIIDRLDG

SEG

2bct- H HHHHHCCCCTTTHHHHHHHHHHHHCTTTHHHHHHHHHTTTHHHHHHHH-HHCH

SEQ VRLLWSLLKNPHPDVKASAAWALCPCIKNAKDAGEMVRSFVGGLELIVNLLKSDNKEVLA

SEG

2bct- HHHHHHHHHTTTHHHHHHHHHHHHHHHCCCCHH-HHHHHHHHHHHHHHHHCTTTTTHHHH

SEQ SVCAAITNIAKDQENLAVITDHGVVPLLSKLANTNNNKLRHHLAEAISRCCMWGRNRVAF

SEG

2bct- HHHHHHHHHHHCGGGHHHHHHHCHHHHHHHHHHHHHHTTTCCHHHHHHHHHHHHCHHHHH

SEQ GEHKAVAPLVRYLKSNDTNVHRATAQALYQLSEDADNCITMHENGAVKLLLDMVGSPDQD

SEG

2bct- HTTTHHHHHHHHHCCCCHHHHHHHHHHHHHHHTTHHHHHHHHHCCHHHHHHHTTTTTTHH

SEQ LQEAAAGCISNIRRLALATEKARYT

SEG

2bct- HHHHHHHHH

(No Prosite data available for DKFZphtes3_35pl7.3) (No Pfam data available for DKFZphtes3_35pl7.3)

DKFZphtes3_35p22

group: cell cycle

DKFZphtes3_35p22 encodes a novel 549 amino acid protein, with similarity to oncogene 1 (tre-2 locus) .

The novel protein is closely raleted to human tre-2 and other enzymes involved in the degradation of ubiquitinated proteins. The human tre-2 oncogene encodes a deubiquitmating enzyme, indicating a role for the ubiquitm system in mammalian growth control.

The novel protein can find application in cancer diagnostics and treatment, and in regulating protein stability and growth control via regulation of ubiquitination. strong similarity to oncogene 1 (tre-2 locus) membrane regions: 1 complete cDNA, complete eds, EST hits

Sequenced by DKFZ

Locus: map="17"

Insert length: 2072 bp

Poly A stretch at pos. 2062, polyadenylation signal at pos. 2039

1 GTTACACACA GGCAGTGGTA TCTGTGAGCA GCTCTGTGGA CTCAAAGGTT 51 TTCTCCCTGA GAGGCATGAC CCAGGCCAGC TGATTCATCA GAATCAGGAT

101 GGACGTGGTA GAGGTCGCGG GCAGTTGGTG GGCACAAGAG CGAGAGGACA

151 TCATTATGAA ATACGAAAAG GGACACCGAG CTGGGCTGCC AGAGGACAAG

201 GGGCCTAAGC CTTTTCGAAG CTACAACAAC AACGTCGATC ATTTGGGGAT

251 TGTACATGAG ACGGAGCTGC CTCCTCTGAC TGCGCGGGAG GCGAAGCAAA

301 TTCGGCGGGA GATCAGCCGA AAGAGCAAGT GGGTGGATAT GCTGGGAGAC

351 TGGGAGAAAT ACAAAAGCAG CAGAAAGCTC ATAGATCGAG CGTACAAGGG

401 AATGCCCATG AACATCCGGG GCCCGATGTG GTCAGTCCTC CTGAACACTG

451 AGGAAATGAA GTTGAAAAAC CCCGGAAGAT ACCAGATCAT GAAGGAGAAG

501 GGCAAGAAGT CATCTGAGCA CATCCAGCGC ATCGACCGGG ACGTAAGCGG

551 GACATTAAGG AAGCATATAT TCTTCAGGGA TCGATACGGA ACCAAGCAGC

601 GGGAACTACT CCACATCCTC CTGGCATATG AGGAGTACAA CCCGGAGGTG

651 GGCTACTGCA GGGACCTGAG CCACATCGCC GCCTTGTTCC TCCTCTATCT

701 TCCTGAGGAG GATGCATTCT GGGCACTGGT GCAGCTGCTG GCCAGTGAGA

751 GGCACTCCCT GCAGGGATTT CACAGCCCAA ATGGCGGGAC CGTCCAGGGG

801 CTCCAAGACC AACAGGAGCA TGTGGTAGCC ACGTCACAAC CCAAGACCAT

851 GGGGCATCAG GACAAGAAAG ATCTATGTGG GCAGTGTTCC CCGTTAGGCT

901 GCCTCATCCG GATATTGATT GACGGGATCT CTCTCGGGCT CACCCTGCGC

951 CTGTGGGACG TGTATCTGGT AGAAGGCGAA CAGGCGCTGA TGCCGATAAC 1001 AAGAATCGCC TTTAAGGTTC AGCAGAAGCG CCTCACGAAG ACGTCCAGGT 1051 GTGGCCCGTG GGCACGTTTT TGCAACCGGT TCGTTGATAC CTGGGCCAGG 1101 GATGAGGACA CTGTGCTCAA GCATCTTAGG GCCTCTATGA AGAAACTAAC 1151 AAGAAAGAAG GGGGACCTGC CACCCCCAGC CAAACCCGAG CAAGGGTCGT 1201 CGGCATCCAG GCCTGTGCCG GCTTCACGTG GCGGGAAGAC CCTCTGCAAG 1251 GGGGACAGGC AGGCCCCTCC AGGCCCACCA GCCCGGTTCC CGCGGCCCAT 1301 TTGGTCAGCT TCCCCGCCAC GGGCACCTCG TTCTTCCACA CCCTGTCCTG 1351 GTGGGGCTGT CCGGGAAGAC ACCTACCCTG TGGGCACTCA GGGTGTGCCC 1401 AGCCCGGCCC TGGCTCAGGG AGGACCTCAG GGTTCCTGGA GATTCCTGCA 1451 GTGGAACTCC ATGCCCCGCC TCCCAACGGA CCTGGACGTA GAGGGCCCTT 1501 GGTTCCGCCA TTATGATTTC AGACAGAGCT GCTGGGTCCG TGCCATATCC 1551 CAGGAGGACC AGCTGGCCCC CTGCTGGCAG GCTGAACACC CTGCGGAGCG 1601 GGTGAGATCG GCTTTCGCTG CACCCAGCAC TGATTCCGAC CAGGGCACCC 1651 CCTTCAGAGC TAGGGACGAA CAGCAGTGTG CTCCCACCTC AGGGCCTTGC 1701 CTCTGCGGCC TCCACTTGGA AAGTTCTCAG TTCCCTCCAG GCTTCTAGAA 1751 GCATCTGGGC CAGGGCTCAT GGCTGGATAA TTTCCCTAGG CTTAACAACC 1801 CAAGCAAGCT TCGCATCCTC GTTTTATTTT TGGTTAAACT TATGAAAATG 1851 TATTAAGAAA GAGTGCAGCT CGAGAGAGAT TCAGAGATGG AACACACCAG 1901 ACCCCAGATC ACAAAGCCAA CCATGCCCAG CCCCTCCCAG CACCCCCAGC 1951 CCCACGACCA TCGTTCTGAA TTCTGACGAC ACCGTGAGCC TGCCTTTGTA 2001 CTTCAAACTC ATGGAAGGAT AACCACCTTC ATGTTTTGAA ATAAATGTTT 2051 CCTGTTGAAA TGAAAAAAAA AA

BLAST Results

Entry AC003976 from database EMBL:

Homo sapiens chromosome 17, clone hCIT.91_J_4, complete sequence.

Score = 4385, P = O.Oe+00, identities = 881/886 14 exons

Entry HSG19723 from database EMBL: human STS A001W35. Score = 850, P = 1.9e-32, identities = 170/170

Medline entries

92228503:

A novel transcriptional unit of the tre oncogene widely expressed in human cancer cells.

94067315:

The yeast DOA4 gene encodes a deubiquitmating enzyme related to a product of the human tre-2 oncogene.

95176708:

UBP5 encodes a putative yeast ubiquitin-specific protease that is related to the human Tre-2 oncogene product.

Peptide information for frame 3

ORF from 99 bp to 1745 bp; peptide length: 549 Category: strong similarity to known protein

1 MDWEVAGSW WAQEREDIIM KYEKGHRAGL PEDKGPKPFR SYNNNVDHLG 51 IVHETELPPL TAREAKQIRR EISRKSKWVD MLGDWEKYKS SRKLIDRAYK 101 GMPMNIRGPM WSVLLNTEEM KLKNPGRYQI MKEKGKKSSE HIQRIDRDVS 151 GTLRKHIFFR DRYGTKQREL LHILLAYEEY NPEVGYCRDL SHIAALFLLY 201 LPEEDAFWAL VQLLASERHS LQGFHSPNGG TVQGLQDQQE HVVATSQPKT 251 MGHQDKKDLC GQCSPLGCLI RILIDGISLG LTLRLWDVYL VEGEQALMPI 301 TRIAFKVQQK RLTKTSRCGP WARFCNRFVD TWARDEDTVL KHLRASMKKL 351 TRKKGDLPPP AKPEQGSSAS RPVPASRGGK TLCKGDRQAP PGPPARFPRP 401 IWSASPPRAP RSSTPCPGGA VREDTYPVGT QGVPSPALAQ GGPQGSWRFL 451 QWNSMPRLPT DLDVEGPWFR HYDFRQSCWV RAISQEDQLA PCWQAEHPAE 501 RVRSAFAAPS TDSDQGTPFR ARDEQQCAPT SGPCLCGLHL ESSQFPPGF

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_35p22, frame 3

PIR:Ξ22155 oncogene 1 (tre-2 locus) (clone 210) - human, N = 1, Score = 2181, P = 5.5e-226

PIR:S57867 oncogene 1 - human, N = 1, Score = 1536, P = 1.2e-157

>PIR:S22155 oncogene 1 (tre-2 locus) (clone 210) - human Length = 786

HSPs:

Score = 2181 (327.2 bits), Expect = 5.5e-226, P = 5.5e-226 Identities = 405/500 (81%), Positives = 440/500 (88%)

Query: 1 MDVVEVAGSWWAQEREDIIMKYEKGHRAGLPEDKGPKPFRSYNNNVDHLGIVHETELPPL 60

MD+VE A S AQER+DI+MKY+KGHRAGLPEDKGP+P N+++D GI+HETELPP+ Sb_jct: 1 MDMVENADSLQAQERKDILMKYDKGHRAGLPEDKGPEPV-GINΞSIDRFGILHETELPPV 59

Query: 61 TAREAKQIRREISRKSKWVDMLGDWEKYKSSRKLIDRAYKGMPMNIRGPMWSVLLNTEEM 120

TAREAK+IRRE++R SKW++MLG+WE YK S KLIDR YKG+PMNIRGP+WSVLLN +E+ Sb_jct: 60 TAREAKKIRREMTRTSKWMEMLGEWETYKHSSKLIDRVYKGIPMNIRGPVWSVLLNIQEI 119

Query: 121 KLKNPGRYQIMKEKGKKSSEHIQRIDRDVSGTLRKHIFFRDRYGTKQRELLHILLAYEEY 180

KLKNPGRYQIMKE+GK+SΞEHI ID DV TLR H+FFRDRYG KQREL +ILLAY EY Sb_jct: 120 KLKNPGRYQIMKERGKRSSEHIHHIDLDVRTTLRNHVFFRDRYGAKQRELFYILLAYSEY 179

Query: 181 NPEVGYCRDLSHIAALFLLYLPEEDAFWALVQLLASERHSLQGFHSPNGGTVQGLQDQQE 240

NPEVGYCRDLSHI ALFLLYLPEEDAFWALVQLLASERHSL GFHSPNGGTVQGLQDQQE Sb_jct: 180 NPEVGYCRDLSHITALFLLYLPEEDAFWALVQLLASERHSLPGFHSPNGGTVQGLQDQQE 239 Query: 241 HVVATSQPKTMGHQDKKDLCGQCSPLGCLIRILIDGISLGLTLRLWDVYLVEGEQALMPI 300

HVV SQPKTM HQDK+ LCGQC+ LGCL+R LIDGISLGLTLRLWDVYLVEGEQ LMPI Sb_jct: 240 HVVPKSQPKTMWHQDKEGLCGQCASLGCLLRNLIDGISLGLTLRLWDVYLVEGEQVLMPI 299

Query: 301 TRIAFKVQQKRLTKTΞRCGPWARFCNRFVDTWARDEDTVLKHLRASMKKLTRKKGDLPPP 360

T IA KVQQKRL KTSRCG WAR N+F DTWA ++DTVLKHLRAS KKLTRK+GDLPPP Sb_jct: 300 TSIALKVQQKRLMKTSRCGLWARLRNQFFDTWAMNDDTVLKHLRASTKKLTRKQGDLPPP 359

Query: 361 AKPEQGSSASRPVPASRGGKTLCKGDRQAPPGPPARFPRPIWSASPPRAPRSSTPCPGGA 420

AK EQGS A RPVPASRGGKTLCKG RQAPPGPPA+F RPI SASPP A R STPCPGGA Sbjct: 360 AKREQGSLAPRPVPASRGGKTLCKGYRQAPPGPPAQFQRPICSASPPWASRFSTPCPGGA 419

Query: 421 VREDTYPVGTQGVPSPALAQGGPQGSWRFLQWNSMPRLPTDLDVEGPWFRHYDFRQSCWV 480

VREDTYPVGTQGVPS ALAQGGPQGSWRFL+W SMPRLPTDLD+ GPWF HYDF +SCWV Sbjct: 420 VREDTYPVGTQGVPSLALAQGGPQGSWRFLEWKΞMPRLPTDLDIGGPWFPHYDFERSCWV 479

Query: 481 RAISQEDQLAPCWQAEHPAE 500

RAISQEDQLA CWQAEH E Sbjct: 480 RAISQEDQLATCWQAEHCGE 499

Pedant information for DKFZphtes3_35p22, frame 3

Report for DKFZphtes3_35p22.3

[LENGTH] 549

[MW] 62159.16

[pi] 9.23

[HOMOL] PIR:S22155 oncogene 1 (tre-2 locus) (clone 210) - human 0.0

[FUNCAT] 11.01 stress response [S. cerevisiae, YGRlOOw] 2e-16

[FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YGRlOOw] 2e-16

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YNL293w] 3e-15

[PIRKW] transmembrane protein 6e-14

[PROSITE] MYRISTYL 6

[PROSITE] AMIDATION 1

[PROSITE] CAMP_PHOSPHO_SITE 3

[PROSITE] CK2_PHOSPHO_SITE 4

[PROSITE] TYR_PHOSPHO_SITE 2

[PROSITE] PKC_PHOSPHO_SITE 10

[KW] TRANSMEMBRANE 1

[KW] LOW COMPLEXITY 5.28 %

SEQ MDVVEVAGSWWAQEREDIIMKYEKGHRAGLPEDKGPKPFRSYNNNVDHLGIVHETELPPL SEG PRD ccceeeccchhhhhhhhhhhhhhccccccccccccccceeeeeccccccccccccccccc MEM

SEQ TAREAKQIRREISRKSKWVDMLGDWEKYKSSRKLIDRAYKGMPMNIRGPMWSVLLNTEEM SEG PRD chhhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhcccccccccceeeccccccc MEM

SEQ KLKNPGRYQIMKEKGKKSSEHIQRIDRDVSGTLRKHIFFRDRYGTKQRELLHILLAYEEY SEG PRD ccccccchhhhhhhccccchhhhhhhhhhhhccccccccccccccchhhhhhhhhhhhhc MEM

SEQ NPEVGYCRDLSHIAALFLLYLPEEDAFWALVQLLASERHSLQGFHSPNGGTVQGLQDQQE SEG PRD ccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccchhhhhhhhhh MEM

SEQ HVVATSQPKTMGHQDKKDLCGQCSPLGCLIRILIDGISLGLTLRLWDVYLVEGEQALMPI SEG PRD hhhhhhhchhhhhhhhccccccccchhhhhhhhhhccccchhhhhhhhhccccceeeehh MEM MMMMMMMMMMMMMMMMMM

SEQ TRIAFKVQQKRLTKTSRCGPWARFCNRFVDTWARDEDTVLKHLRASMKKLTRKKGDLPPP SEG PRD hhhhhhhhhhhhhhhcccchhhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhhhcccc MEM

SEQ AKPEQGSSASRPVPASRGGKTLCKGDRQAPPGPPARFPRPIWSASPPRAPRSSTPCPGGA SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxx ... PRD ccccccccccccccccccceeeeccccccccccccccccccccccccccccccccccccc MEM SEQ VREDTYPVGTQGVPSPALAQGGPQGSWRFLQWNSMPRLPTDLDVEGPWFRHYDFRQSCWV SEG PRD cccccccccccccccccccccccccceeeeeccccccccccccccccccccccccccccc MEM

SEQ RAISQEDQLAPCWQAEHPAERVRSAFAAPSTDSDQGTPFRARDEQQCAPTSGPCLCGLHL SEG PRD cchhhhhhhhhhhhhhcchhhhhhhhccccccccccccccchhhhhcccccccccceeee MEM

SEQ ESSQFPPGF SEG PRD ccccccccc MEM

Prosite for DKFZphtes3_35p22.3

PS00004 136->140 CAMP_PHOSPHO_SITE PDOC00004 PS00004 310->314 CAMP_PHOSPHO_SITE PDOC00004 PS00004 348->352 CAMP_PHOSPHO_SITE PDOC00004 PS00005 61->64 PKC_PHOSPHO_SITE PDOC00005 PS00005 73->76 PKC_PHOSPHO_SITE PDOC00005 PS00005 90->93 PKC_PHOSPHO_SITE PDOC00005 PS00005 152->155 PKC_PHOSPHO_SITE PDOC00005 PS00005 216->219 PKC_PHOSPHO_SITE PDOC00005 PS00005 282->285 PKC_PHOSPHO_SITE PDOC00005 PS00005 315->318 PKC_PHOSPHO_SITE PDOC00005 PS00005 346->349 PKC_PHOSPHO_SITE PDOC00005 PS00005 351->354 PKC_PHOSPHO_SITE PDOC00005 PS00005 446->449 PKC_PHOSPHO_SITE PDOC00005 PS00006 61->65 CK2_PHOSPHO_SITE PDOC00006 PS00006 460->464 CK2_PHOSPHO_SITE PDOC00006 PS00006 484->488 CK2_PHOSPHO_SITE PDOC00006 PS00006 511->515 CK2_PHOSPHO_SITE PDOC00006 PS00007 93->100 TYR_PHOSPHO_SITE PDOC00007 PS00007 92->100 TYR_PHOΞPHO_SITE PDOC00007 PS00008 8->14 MYRISTYL PDOC00008 PS00008 101->107 MYRISTYL PDOC00008 PS00008 230->236 MYRISTYL PDOC00008 PS00008 276->282 MYRISTYL PDOC00008 PS00008 366->372 MYRISTYL PDOC00008 PS00008 441->447 MYRISTYL PDOC00008 PS00009 134->138 AMIDATION PDOC00009

(No Pfam data available for DKFZphtes3_35p22.3)

DKFZphtes3_4b4

group: testes derived

DKFZphtes3_4b4 encodes a novel 497 ammo acid protein similar to SCP proteins and a human trypsin inhibitor.

The novel protein contains an extracellular proteins SCP/Tpx-l/Ag5/PR-l/Sc7 signature 2, predicted by Prosite and Pfam. This domain is found in a variety of extracellular proteins from eukaryotes that have been found to be evolutionary related. The exact function of these proteins is not yet known. In addition, the protein is similar to a human trypsin inhibitor.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application m studying the expression profile of testis-specific genes or as a new protease inhibitor. strong similarity to trypsin inhibitor might be a new protease inhibitor⁷

Sequenced by AGOWA

Locus: /map="333.4 cR from top of Chrl6 linkage group"

Insert length: 4574 bp

Poly A stretch at pos. 4551, polyadenylation signal at pos. 4539

1 GGCGGCTGCT CCCATTGAGC TGTCTGCTCG CTGTGCCCGC TGTGCCTGCT

51 GTGCCCGCGC TGTCGCCGCT GCTACCGCGT CTGCTGGACG CGGGAGACGC

101 CAGCGAGCTG GTGATTGGAG CCCTGCGGAG AGCTCAAGCG CCCAGCTCTG

151 CCCGAGGAGC CCAGGCTGCC CCGTGAGTCC CATAGTTGCT GCAGGAGTGG

201 AGCCATGAGC TGCGTCCTGG GTGGTGTCAT CCCCTTGGGG CTGCTGTTCC

251 TGGTCTGCGG ATCCCAAGGC TACCTCCTGC CCAACGTCAC TCTCTTAGAG

301 GAGCTGCTCA GCAAATACCA GCACAACGAG TCTCACTCCC GGGTCCGCAG

351 AGCCATCCCC AGGGAGGACA AGGAGGAGAT CCTCATGCTG CACAACAAGC

401 TTCGGGGCCA GGTGCAGCCT CAGGCCTCCA ACATGGAGTA CATGACCTGG

451 GATGACGAAC TGGAGAAGTC TGCTGCAGCG TGGGCCAGTC AGTGCATCTG

501 GGAGCACGGG CCCACCAGTC TGCTGGTGTC CATCGGGCAG AACCTGGGCG

551 CTCACTGGGG CAGGTATCGC TCTCCGGGGT TCCATGTGCA GTCCTGGTAT

601 GACGAGGTGA AGGACTACAC CTACCCCTAC CCGAGCGAGT GCAACCCCTG

651 GTGTCCAGAG AGGTGCTCGG GGCCTATGTG CACGCACTAC ACACAGATAG

701 TTTGGGCCAC CACCAACAAG ATCGGTTGTG CTGTGAACAC CTGCCGGAAG

751 ATGACTGTCT GGGGAGAAGT TTGGGAGAAC GCGGTCTACT TTGTCTGCAA

801 TTATTCTCCA AAGGGGAACT GGATTGGAGA AGCCCCCTAC AAGAATGGCC

851 GGCCCTGCTC TGAGTGCCCA CCCAGCTATG GAGGCAGCTG CAGGAACAAC

901 TTGTGTTACC GAGAAGAAAC CTACACTCCA AAACCTGAAA CGGACGAGAT

951 GAATGAGGTG GAAACGGCTC CCATTCCTGA AGAAAACCAT GTTTGGCTCC

1001 AACCGAGGGT GATGAGACCC ACCAAGCCCA AGAAAACCTC TGCGGTCAAC

1051 TACATGACCC AAGTCGTCAG ATGTGACACC AAGATGAAGG ACAGGTGCAA

1101 AGGGTCCACG TGTAACAGGT ACCAGTGCCC AGCAGGCTGC CTGAACCACA

1151 AGGCGAAGAT CTTTGGAACT CTGTTCTATG AAAGCTCGTC TAGCATATGC

1201 CGCGCCGCCA TCCACTACGG GATCCTGGAT GACAAGGGAG GCCTGGTGGA

1251 TATCACCAGG AACGGGAAGG TCCCCTTCTT CGTGAAGTCT GAGAGACACG

1301 GCGTGCAGTC CCTCAGCAAA TACAAACCTT CCAGCTCATT CATGGTGTCA

1351 AAAGTGAAAG TGCAGGATTT GGACTGCTAC ACGACCGTTG CTCAGCTGTG

1401 CCCGTTTGAA AAGCCAGCAA CTCACTGCCC AAGAATCCAT TGTCCGGCAC

1451 ACTGCAAAGA CGAACCTTCC TACTGGGCTC CGGTGTTTGG AACCAACATC

1501 TATGCAGATA CCTCAAGCAT CTGCAAGACA GCCGTGCACG CGGGAGTCAT

1551 CAGCAACGAG AGTGGGGGTG ACGTGGACGT GATGCCCGTG GATAAAAAGA

1601 AGACCTACGT GGGCTCGCTC AGGAATGGAG TTCAGTCTGA AAGCCTGGGG

1651 ACTCCTCGGG ATGGAAAGGC CTTCCGGATC TTTGCTGTCA GGCAGTGAAT

1701 TTCCAGCACC AGGGGAGAAG GGGCGTCTTC AGGAGGGCTT CGGGGTTTTG

1751 CTTTTATTTT TATTTTGTCA TTGCGGGGTA TATGGAGAGT CAGGAAACTT

1801 CCTTTGACTG ATGTTCAGTG TCCATCACTT TGTGGCCTGT GGGTGAGGTG

1851 ACATCTCATC CCCTCACTGA AGCAACAGCA TCCCAAGGTG CTCAGCCGGA

1901 CTCCCTGGTG CCTGATCCTG CTGGGGCCCG GGGGTCTCCA TCTGGACGTC

1951 CTCTCTCCTT TAGAGATCTG AGCTGTCTCT TAAAGGGGAC AGTTGCCCAA

2001 AATGTTCCTT GCTATGTGTT CTTCTGTTGG TGGAGGAAGT TGATTTCAAC

2051 CTCCCTGCCA AAAGAACAAA CCATTTGAAG CTCACAATTG TGAAGCATTC

2101 ACGGCGTCGG AAGAGGCCTT TTGAGCAAGC GCCAATGAGT TTCAGGAATG

2151 AAGTAGAAGG TAGTTATTTA AAAATAAAAA ACACAGTCCG TCCCTACCAA

2201 TAGAGGAAAA TGGTTTTAAT GTTTGCTGGT CAGACAGACA AATGGGCTAG

2251 AGTAAGAGGG CTGCGGGTAT GAGAGACCCC GGCTCCGCCC TGGCACGTGT

2301 CCTTGCTGGC GGCCCGCCAC AGGCCCCCTT CAATGGCCGC ATTCAGGATG

2351 GCTCTATACA CAGCAGTGCT GGTTTATGTA GAGTTCAGCA GTCACTTCAG

2401 AGATGTATCT TGTCTTTGTC AGGCCCTTCA TCTTCATGGC CCACCTGTTT

2451 TCTGCCGTGA CCTTTGGTCC CATTGAGGAC TAAGGATCGG GACCCTTTCT 2501 TTACCCCCTA CCCATTGTGG CTCCCACCCT GCCTCGGACT GGTTTACGTG 2551 TCCTGGTTCA CACCCAGGAC TTTTCTTTGC AAGCGAACCT GTTTGAAGCC 2601 CAAGTCTTAA CTCCTGGTCT CGTAAGGTTC CACTGAGACG AGATGTCTGA 2651 GAACAACCAA AGAAGGCCTG CTCTTTGCTG CTTTTAAAAA ATGACAATTA 2701 AATGTGCAGA TTCCCCACGC ACCCGATGAC CTATTTTTTC AGCCGTGGGA 2751 GGAATGGAGT CTTTGGTACA TTCCTCACCG AGGTTAGCAG CTCAGTTTGT 2801 GGTTATGAAA CCGTCTGTGG CCTCATGACA GCGAGAGATG GGAATACACT 2851 AGAAGGATCT CTTTTCCTGT TTTCGTGAAA CGACTCTTGC CAAACGTTCC 2901 CGAGGCGCCA AGGAGTGTAG TACACCCTGG CTGCCATCAC TCTATAAAAG 2951 TGCTTCATGA GCCCAGACCA AAAGCCCACA GTGAAATGAA GTACCCTTTT 3001 GTAAATAGCA TTTTTTTGCA GAAGGTGAAA ATTCCACTCT CTACCACCGG 3051 GCCAGCCAAT AGATCACTTT GGTGAATGCT AGTTTCAAAT TTGATTCAAA 3101 ATATTTCTTA GGTGAAAGAA CTAGCAGAAA GTCAAAAACT AAGATACTGT 3151 AGACTGGACA AGAAATTCTA CCTGGGCACC TAGGTGATGC CTTCTTTCTT 3201 TGATTGCCTT TCTAATAAAT GCAGAATCTG AAGGTAAATA GGTTTAAAAC 3251 AAAACAAAAA CCCACCCCTT TAAGGAGTTG GTAAAAAGCA GTTCAACTCT 3301 TAGCTTGACT GAGCTAAAAT TCACAGGACT ACGTGCTTTG TGCATTGTAG 3351 TCTAGTCGTA ATTCATAGGT ACTGACTCCT CAGCCCCAAA TGTCGGAGAG 3401 GAAGAATTCG GTCAGCCTGT CAGGTCGTGA GTCCAGTTAC CACCAAACAT 3451 CTGGGAAACT TCTGGGTGCT GGGTGCTCTG CTGCTGGACT TTTGTGGCTG 3501 TGTCTGTGTC TGCAAGATAA ATTAGATCGC CCTGTGGGGT TTGCAGAATT 3551 AGTGAAGGGT CCAGGACGAT CCCAGTGGGC TCGCTTCCAA AGCATCCCAC 3601 TCAAGGGAGA CTTGAAACTT CCAGTGTGAG TTGACCCCAT CATTTAAAAA 3651 TAAAGTCCCC GGGTTCCTTA ATGCCTCCTT CACTGGGCCT TCCTAGCAGG 3701 ATAGAAAGTC CTTGCCCAGA GCAGGACCTG GCTGTCTTTT TTTTTTTTTT 3751 TTTCCCGAGA CCAAGTTTCA CTCTGTTGCC CAAGGTAGAG TGCAGTGGCG 3801 TGATCTCTGC TCATTGCAAC TGCCGCCTCC CGGGTTCAAG CAATTCTCAT 3851 GCATCAGCCT CCCAAGTACC TGGGACTACA GGCGTGAGCT ACCATGCCCG 3901 GCTAATTTTT GTATTTTTAG TAGAGATGGG GTTTCATTAT GTTGGCCAGG 3951 CTGGTCTCGA ACTCCTTACC TCAGGTGATC CACCCACCTT GGCCTCCCGA 4001 AGTGCTGGGA TTACAGGCAT GAGCCACTGC GCCCGGCCAT GGACCTGGCT 4051 GTCTTTATCA TCCCCACAAA CATTTTGAAA CTGGAATATT TGTCTTCAGA 4101 AAATGGAAAC AAGACTATAA ATGATAAGCC CTGTCCCTAG CACCACCTCT 4151 CCTGTGTGTG GAATAGAGGC CCCTCGTGCT ACCAACACTT ACCCTGTGTT 4201 TAAAAAGATC TTGTACCAAG CCAACGGCGT TCCTGGCTCT CCTGCCCACA 4251 GGATGAACAT TTTCGGCTTC CTTAGGAGTT TTGCCCTACC GTATTCCAAA 4301 GCGTGTGCTG GTTTCTCATA TTGTCTGTAG GCTCACTCAG CCCGCAGTTT 4351 ATGTGTGTGC TTTTTTCTAT GAAAAATGAT GTATTTTGCT ACTTCCTGTG 4401 TACAAAGTTT TATTGTAAAT GTTTTTTGTG CTTTGCATGA ACAGGGGCCA 4451 CGTTGTTGCA ATTGTTTCAG TAGAACTGGT TTGATTTCTA AAATGTTCCT 4501 GTAACATATC TTTTATGAAC AAATCTGAAC AATTTGTGAA ATAAAACATT 4551 GAAAACCAAA AAAAAAAAAA AAAA

BLAST Results

Entry HS834352 from database EMBL : human STS WI -15502 . Score = 1331 , P = 5 . 4e- 54 , identities = 287 /301

Medline entries

98146272: cDNA cloning of a novel trypsin inhibitor with similarity to pathogenesis-related proteins, and its frequent expression in human brain cancer cells.

Peptide information for frame 1

ORF from 205 bp to 1695 bp; peptide length: 497 Category: strong similarity to known protein

1 MSCVLGGVIP LGLLFLVCGS QGYLLPNVTL LEELLSKYQH NESHSRVRRA

51 IPREDKEEIL MLHNKLRGQV QPQASNMEYM TWDDELEKSA AAWASQCIWE

101 HGPTSLLVSI GQNLGAHWGR YRSPGFHVQS WYDEVKDYTY PYPSECNPWC

151 PERCSGPMCT HYTQIVWATT NKIGCAVNTC RKMTVWGEVW ENAVYFVCNY

201 SPKGNWIGEA PYKNGRPCSE CPPSYGGSCR NNLCYREETY TPKPETDEMN

251 EVETAPIPEE NHVWLQPRVM RPTKPKKTSA VNYMTQVVRC DTKMKDRCKG

301 STCNRYQCPA GCLNHKAKIF GTLFYESSSS ICRAAIHYGI LDDKGGLVDI

351 TRNGKVPFFV KSERHGVQSL SKYKPSSSFM VSKVKVQDLD CYTTVAQLCP

401 FEKPATHCPR IHCPAHCKDE PSYWAPVFGT NIYADTSSIC KTAVHAGVIS 451 NESGGDVDVM PVDKKKTYVG SLRNGVQSES LGTPRDGKAF RIFAVRQ

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_4b4, frame 1

TREMBLNEW:AF109674_1 gene: "Lgll"; product: "late gestation lung protein 1"; Rattus norvegicus late gestation lung protein 1 (Lgll) mRNA, complete eds., N = 1, Score = 968, P = 1.9e-97

TREMBL :D45027_1 product: "25 kDa trypsin inhibitor"; Homo sapiens mRNA for 25 kDa trypsin inhibitor, complete eds., N = 1, Score = 738, P = 4.5e-73

TREMBL :AB009609_1 gene: "HrTT-1"; Halocynthia roretzi HrTT-1 mRNA, complete eds., N = 1, Score = 345, P = 2e-31

PIR:JC5308 testis-specific, vespid, and pathogenesis-related protein 1 precursor - human, N = 1, Score = 337, P = 1.7e-30

>TREMBLNEW:AF109674_1 gene: "Lgll"; product: "late gestation lung protein

1"; Rattus norvegicus late gestation lung protein 1 (Lgll) mRNA, complete eds .

Length = 188

HSPs:

Score = 968 (145.2 bits), Expect = 1.9e-97, P = 1.9e-97 Identities = 160/185 (86%), Positives = 170/185 (91%)

Query: 61 MLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGR 120

MLHNKLRGQV P ASNMEYMTWD+ELE+SAAAWA +C+WEHGP SLLVSIGQNL HWGR Sbjct: 1 MLHNKLRGQVYPPASNMEYMTWDEELERSAAAWAQRCLWEHGPASLLVSIGQNLAVHWGR 60

Query: 121 YRSPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTC 180

YRSPGFHVQSWYDEVKDYTYPYP ECNPWCPERCSG MCTHYTQ+VWATTNKIGCAV+TC Sbjct: 61 YRSPGFHVQSWYDEVKDYTYPYPHECNPWCPERCSGAMCTHYTQMVWATTNKIGCAVHTC 120

Query: 181 RKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETY 240

R M+VWG++WENAVY VCNYSPKGNWIGEAPYK+GRPCSECP SYGG CRNNLCYREE Y Sbjct: 121 RSMSVWGDIWENAVYLVCNYSPKGNWIGEAPYKHGRPCSECPSSYGGGCRNNLCYREEHY 180

Query: 241 TPKPE 245

KPE Sbjct: 181 HQKPE 185

Pedant information for DKFZphtes3_4b4, frame 1

Report for DKFZphtes3_4b4.1

[LENGTH] 497

[MW] 55920.00

[pi] 8.36

[HOMOL] TREMBL :D45027_1 product: "25 kDa trypsin inhibitor"; Homo sapiens mRNA for 25 kDa trypsin inhibitor, complete eds. 6e-78

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YJL078c] 8e-12

[BLOCKS] BL01009E Extracellular proteins ΞCP/Tpx-l/Ag5/PR-l/Sc7 proteins

[BLOCKS] BL01009D Extracellular proteins SCP/Tpx-l/Ag5/PR-l/Sc7 proteins

[BLOCKS] BL01009C Extracellular proteins SCP/Tpx-l/Ag5/PR-l/Sc7 proteins

[BLOCKS] BL01009A Extracellular proteins SCP/Tpx-l/Ag5/PR-l/Sc7 proteins

[PIRKW] glycoprotein 5e-22

[PIRKW] blocked ammo end 5e-13

[PIRKW] brain 9e-30

[PIRKW] hydrolase 4e-09

[PIRKW] hemolymph coagulation 4e-09

[PIRKW] zymogen 4e-09

[PIRKW] alternative splicing 4e-09

[PIRKW] sperm 5e-₂2

[PIRKW] viroid-induced protein 2e-ll

[PIRKW] venom 6e-18

[PIRKW] pyroglutamic acid 2e-ll

[PIRKW] transmembrane protein 2e-10

[PIRKW] serine proteinase 4e-09

[SUPFAM] C-type lectin homology 4e-09

[SUPFAM] trypsin homology 4e-09 [SUPFAM] complement factor H repeat homology 4 e-09

[SUPFAM] cyste e-rich secretory protein 1 6e-24

[SUPFAM] pathogenesis -related leaf pr otein 7e-15

[PROSITE] MYRISTYL 8

[PROSITE] CAMP_PHOSPHO_SITE 3

[PROSITE] CK2_PHOSPHO_SITE 6

[PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 8

[PROSITE] ASN_GLYCOSYLATION 3

[PROSITE] SCP_AG5_PR1_SC7_2 1

[PFAM] SCP-li ke extracellular Proteins

[KW] All_Beta

[KW] SIGNAL_PEPTIDE 23

[KW] LOW COMPLEXITY 1.21 %

SEQ MΞCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEIL SEG xxxxxx PRD ccceeeeeceeeeeeeecccccccccchhhhhhhhhhhhhcccchhhhhhhccchhhhhh

SEQ MLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGR SEG PRD hhhhhhhcccccccccchhhhhhhhhhhhhhhhhhhhcccccccccccccccceeeeecc

SEQ YRSPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTC SEG PRD ccccchhhhhhhhhhhccccccccccccccccccccccccceeeeeeeccccccceeeec

SEQ RKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETY SEG PRD cccccccccccceeeeeeeccccccccccccccccccccccccccccccccccccccccc

SEQ TPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKG SEG PRD cccccccccccccccccccceeeeecccccccccccceeeeeeeeeeeeecccccccccc

SEQ STCNRYQCPAGCLNHKAKIFGTLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFV SEG PRD ccccccccccccccccceeeeeeeeecccceeeeeccccccccccceeeeeccccceeee

SEQ KSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDE SEG PRD eccceeeeeeeeccccceeeeeeeeeecccceeeeeeeeccccccccccccccccccccc

SEQ PSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSES SEG PRD ccceeeeeceeeccccceeeeeeeeccccccccccccceeecccceeeeeecccceeeee

SEQ LGTPRDGKAFRIFAVRQ SEG PRD ccccccccceeeeeccc

Prosite for DKFZphtes3_4b4.1

PS00001 27->31 ASN_GLYCOSYLATION PDOC00001 PS00001 41->45 ASN_GLYCOSYLATION PDOC00001 PS00001 451->455 ASN_GLYCOSYLATION PDOC00001 PS00004 181->185 CAMP_PHOSPHO_SITE PDOC00004 PS00004 276->280 CAMP_PHOSPHO_SITE PDOC00004 PS00004 464->468 CAMP_PHOSPHO_SITE PDOC00004 PS00005 170->173 PKC_PHOSPHO_SITE PDOC00005 PS00005 179->182 PKC_PHOSPHO_SITE PDOC00005 PS00005 201->204 PKC_PHOSPHO_SITE PDOC00005 PS00005 228->231 PKC_PHOSPHO_SITE PDOC00005 PS00005 241->244 PKC_PHOSPHO_SITE PDOC00005 PS00005 362->365 PKC_PHOSPHO_SITE PDOC00005 PS00005 471->474 PKC_PHOSPHO_SITE PDOC00005 PS00005 483->486 PKC_PHOSPHO_SITE PDOC00005 PS00006 29->33 CK2_PHOSPHO_SITE PDOC00006 PS00006 75->79 CK2_PHOSPHO_SITE PDOC00006 PS00006 81->85 CK2_PHOSPHO_SITE PDOC00006 PS00006 130->134 CK2_PHOSPHO_SITE PDOC00006 PS00006 453->457 CK2_PHOSPHO_SITE PDOC00006 PS00006 483->487 CK2_PHOSPHO_SITE PDOC00006 PS00007 385->393 TYR_PHOSPHO_SITE PDOC00007 PS00008 111->117 MYRISTYL PDOC00008 PS00008 115->121 MYRISTYL PDOC00008 PS00008 174->180 MYRISTYL PDOC00008 PS00008 204->210 MYRISTYL PDOC00008 PS00008 227->233 MYRISTYL PDOC00008 PS00008 300->306 MYRISTYL PDOC00008 PS00008 447->453 MYRISTYL PDOC00008 PS00008 470->476 MYRISTYL PDOC00008 PS01010 195->207 SCP AG5 PR1 SC7 2 PDOC00772

Pfam for DKFZphtes3_4b4.1

HMM_NAME SCP-like extracellular Proteins

HMM *PQDEQDEWLNkHNDFRQQVGRGLETRGNPGPQPPAsNMnPMVWNDELAt P + ++E+L HN +R QV P ASNM M+W+DEL +

Query 52 PREDKEEILMLHNKLRGQVQ PQASNMEYMTWDDELEK 88

HMM IAQnWANQCiFDHHDCCWNHsnYPYGQNIAWWSsTANnPWnWssMIQMWY A WA+QCI +H ++ + S GQN+ + + ++++ +Q+WY

Query 89 SAAAWASQCIWEHGPTSLLVSI GQNLGAHWG RYRSPGFHVQΞWY 132

HMM NEvkDYNYNWNTCkGG NNFmVCGHYTQMVWRnTfrIGCGRYICYC

+EVKDY Y + + +C HYTQ+VW+ T +IGC+ C+

Query 133 DEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTCRK 182

HMM NNNWrKPDPWKhkWYYVCNYCPpGNYmN* + W + W+ +Y VCNY P+GN+++

Query 183 MTV —GEVWENAVYFVCNYSPKGNWIG 208

DKFZphtes3_4fl7

group: testes derived

DKFZphtes3_4f17 encodes a novel 656 amino acid protein with weak similarity to methyl-CpG- bindmg proteins.

Methylation at the DNA sequence 5 ' -CpG is required for mammalian development. Methyl-CpG- binding proteins bind specifically to methylated DNA via a related ammo acid motif and can repress transcription. The novel protein does not contain such a motife. No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . similarity to methyl-CpG-bindmg protein extension of HS557771/HSZ78337, there are some differences to these sequences

Sequenced by AGOWA

Locus: /map="18"

Insert length : 2320 bp

Poly A stretch at pos . 2266 , polyadenylation signal at pos . 2251

1 GGCAGGTTCG CGGGTCGCTG GCGGGGGTCG TGAGGGAGTG CGCCGGGAGC 51 GGAGATATGG AGGGAGATGG TTCAGACCCA GAGCCTCCAG ATGCCGGGGA

101 GGACAGCAAG TCCGAGAATG GGGAGAATGC GCCCATCTAC TGCATCTGCC

151 GCAAACCGGA CATCAACTGC TTCATGATCG GGTGTGACAA CTGCAATGAG

201 TGGTTCCATG GGGACTGCAT CCGGATCACT GAGAAGATGG CCAAGGCCAT

251 CCGGGAGTGG TACTGTCGGG AGTGCAGAGA GAAAGACCCC AAGCTAGAGA

301 TTCGCTATCG GCACAAGAAG TCACGGGAGC GGGATGGCAA TGAGCGGGAC

351 AGCAGTGAGC CCCGGGATGA GGGTGGAGGG CGCAAGAGGC CTGTCCCTGA

401 TCCAGACCTG CAGCGCCGGG CAGGGTCAGG GACAGGGGTT GGGGCCATGC

451 TTGCTCGGGG CTCTGCTTCG CCCCACAAAT CCTCTCCGCA GCCCTTGGTG

501 GCCACACCCA GCCAGCATCA CCAGCAGCAG CAGCAGCAGA TCAAACGGTC

551 AGCCCGCATG TGTGGTGAGT GTGAGGCATG TCGGCGCACT GAGGACTGTG

601 GTCACTGTGA TTTCTGTCGG GACATGAAGA AGTTCGGGGG CCCCAACAAG

651 ATCCGGCAGA AGTGCCGGCT GCGCCAGTGC CAGCTGCGGG CCCGGGAATC

701 GTACAAGTAC TTCCCTTCCT CGCTCTCACC AGTGACGCCC TCAGAGTCCC

751 TGCCAAGGCC CCGCCGGCCA CTGCCCACCC AACAGCAGCC ACAGCCATCA

801 CAGAAGTTAG GGCGCATCCG TGAAGATGAG GGGGCAGTGG CGTCATCAAC

851 AGTCAAGGAG CCTCCTGAGG CTACAGCCAC ACCTGAGCCA CTCTCAGATG

901 AGGACCTACC TCTGGATCCT GACCTGTATC AGGACTTCTG TGCAGGGGCC

951 TTTGATGACC ATGGCCTGCC CTGGATGAGC GACACAGAAG AGTCCCCATT 1001 CCTGGACCCC GCGCTGCGGA AGAGGGCAGT GAAAGTGAAG CATGTGAAGC 1051 GTCGGGAGAA GAAGTCTGAG AAGAAGAAGG AGGAGCGATA CAAGCGGCAT 1101 CGGCAGAAGC AGAAGCACAA GGATAAATGG AAACACCCAG AGAGGGCTGA 1151 TGCCAAGGAC CCTGCGTCAC TGCCCCAGTG CCTGGGGCCC GGCTGTGTGC 1201 GCCCCGCCCA GCCCAGCTCC AAGTATTGCT CAGATGACTG TGGCATGAAG 1251 CTGGCAGCCA ACCGCATCTA CGAGATCCTC CCCCAGCGCA TCCAGCAGTG 1301 GCAGCAGAGC CCTTGCATTG CTGAAGAGCA CGGCAAGAAG CTGCTCGAAC 1351 GCATTCGCCG AGAGCAGCAG AGTGCCCGCA CCCGCCTTCA GGAAATGGAA 1401 CGCCGATTCC ATGAGCTTGA GGCCATCATT CTACGTGCCA AGCAGCAGGC 1451 TGTGCGCGAG GATGAGGAGA GCAACGAGGG TGACAGTGAT GACACAGACC 1501 TGCAGATCTT CTGTGTTTCC TGTGGGCACC CCATCAACCC ACGTGTTGCC 1551 TTGCGCCACA TGGAGCGCTG CTACGCCAAG TATGAGAGCC AGACGTCCTT 1601 TGGGTCCATG TACCCCACAC GCATTGAAGG GGCCACACGA CTCTTCTGTG 1651 ATGTGTATAA TCCTCAGAGC AAAACATACT GTAAGCGGCT CCAGGTGCTG 1701 TGCCCCGAGC ACTCACGGGA CCCCAAAGTG CCAGCTGACG AGGTATGCGG 1751 GTGCCCCCTT GTACGTGATG TCTTTGAGCT CACGGGTGAC TTCTGCCGCC 1801 TGCCCAAGCG CCAGTGCAAT CGCCATTACT GCTGGGAGAA GCTGCGGCGT 1851 GCGGAAGTGG ACTTGGAGCG CGTGCGTGTG TGGTACAAGC TGGACGAGCT 1901 GTTTGAGCAG GAGCGCAATG TGCGCACAGC CATGACAAAC CGCGCGGGAT 1951 TGCTGGCCCT GATGCTGCAC CAGACGATCC AGCACGATCC CCTCACTACC 2001 GACCTGCGCT CCAGTGCCGA CCGCTGAGCC TCCTGGCCCG GACCCCTTAC 2051 ACCCTGCATT CCAGATGGGG GAGCCGCCCG GTGCCCGTGT GTCCGTTCCT 2101 CCACTCATCT GTTTCTCCGG TTCTCCCTGT GCCCATCCAC CGGTTGACCG 2151 CCCATCTGCC TTTATCAGAG GGACTGTCCC CGTCGACATG TTCAGTGCCT 2201 GGTGGGGCTG CGGAGTCCAC TCATCCTTGC CTCCTCTCCC TGGGTTTTGT 2251 TAATAAAATT TTGAAGAAAC CAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 2301 AAAAAAAAAA AAAAAAAAAA

BLAST Results Entry HS557771 from database EMBLEST :

Human chromosome 18 clone 2 mRNA sequence.

Score = 7582, P = 0.0e+00, identities = 1560/1598

Entry HSZ78337 from database EMBLEST:

H. sapiens mRNA, expressed sequence tag ICRFp507H02194 (5')

Score = 6339, P = 9.0e-281, identities = 1307/1347

Entry HS095149 from database EMBL: human STS WI-6941. Score = 1210, P = 2.2e-49, identities = 246/251

Medline entries

98449942:

Identification and characterization of a family of mammalian methyl-CpG binding proteins .

9824997:

Gene silencing by methyl-CpG-bmding proteins.

Peptide information for frame 3

ORF from 57 bp to 2024 bp; peptide length: 656 Category: similarity to known protein

1 MEGDGSDPEP PDAGEDSKSE NGENAPIYCI CRKPDINCFM IGCDNCNEWF 51 HGDCIRITEK MAKAIREWYC RECREKDPKL EIRYRHKKSR ERDGNERDSS 101 EPRDEGGGRK RPVPDPDLQR RAGSGTGVGA MLARGSASPH KSSPQPLVAT 151 PSQHHQQQQQ QIKRSARMCG ECEACRRTED CGHCDFCRDM KKFGGPNKIR 201 QKCRLRQCQL RARESYKYFP SSLSPVTPSE SLPRPRRPLP TQQQPQPSQK 251 LGRIREDEGA VASSTVKEPP EATATPEPLS DEDLPLDPDL YQDFCAGAFD 301 DHGLPWMSDT EESPFLDPAL RKRAVKVKHV KRREKKSEKK KEERYKRHRQ 351 KQKHKDKWKH PERADAKDPA SLPQCLGPGC VRPAQPSSKY CSDDCGMKLA 401 ANRIYEILPQ RIQQWQQSPC IAEEHGKKLL ERIRREQQSA RTRLQEMERR 451 FHELEAIILR AKQQAVREDE ESNEGDSDDT DLQIFCVSCG HPINPRVALR 501 HMERCYAKYE ΞQTSFGSMYP TRIEGATRLF CDVYNPQSKT YCKRLQVLCP 551 EHSRDPKVPA DEVCGCPLVR DVFELTGDFC RLPKRQCNRH YCWEKLRRAE 601 VDLERVRVWY KLDELFEQER NVRTAMTNRA GLLALMLHQT IQHDPLTTDL 651 RSSADR

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_4f17, frame 3

TREMBL :CEF52B11_4 gene: "F52B11.1"; Caenorhabditis elegans cosmid F52B11, N = 2, Score = 316, P = 8.8e-27

TREMBL:HSAB2331_1 gene: "KIAA0333"; Human mRNA for KIAA0333 gene, partial eds., N = 2, Score = 163, P = 2.8e-13

TREMBL :SPCC594_5 gene: "SPCC594.05c"; product: "putative transcriptional regulatory protein, phd finger containing"; S. pombe chromosome III cosmid c594. , N = 3, Score = 168, P = 3.6e-12

TREMBL:AF072240_1 gene: "Mbdl"; product: "methyl-CpG binding protein MBDl"; Mus musculus methyl-CpG binding protein MBD1 (Mbdl) mRNA, complete eds., N = 2, Score = 189, P = 7.6e-ll

>TREMBL : CEF52B11_4 gene : ^•F52B11 . 1 ' Caenorhabditis elegans cosmid F52B11 Length = 523

HSPs :

Score = 316 (47.4 bits), Expect = 8.8e-27, Sum P(2) = 3e-27 Identities = 100/336 (29%), Positives = 167/336 (49%) Query: 333 REKKSEKKKEERYKRHRQ-KQKHKDKWKHPERADAKDPASLP-QCLGPGCVRPAQPSSKY 390

+++K+ E Y +R +Q+ D + + +A +P P QCL P C+ ++ SKY Sbjct: 118 QQRKANIINERDYVPNRPTRQQSADLRRKRTQLNA-EPDKHPRQCLNPNCIYESRIDSKY 176

Query: 391 CSDDCGMKLAANRIYEILPQRIQQW QQSPCIAEEHGKKLLERIRREQQSARTRLQ 445

CSD+CG +LA R+ EILP R +Q+ P E+ K +1 RE Q + Sbjct: 177 CSDECGKELARMRLTEILPNRCKQYFFEGPSGGPRSLEDEIKPKRAKINREVQKLTESEK 236

Query: 446 EMERRFHEL-EAIILRAKQQAVREDEESNEGDSDDTDLQIFCVSCGHPINPRVAL-RHME 503

M ++L E I + K Q + +E D +L C+ CG P P + +H+E Sbjct: 237 NMMAFLNKLVEFIKTQLKLQPLGTEERY DDNLYEGCIVCGLPDIPLLKYTKHIE 290

Query: 504 RCYAKYESQTSFGSMYPTRIEGATRLFCDVYNPQSKTYCKRLQVLCPEHSRDPKVPADEV 563

C+A+ E SFG+ P + +C+ Y+ ++ ++CKRL+ LCPEH + +V Sbjct: 291 LCWARSEKAISFGA—PEK--NNDMFYCEKYDSRTNSFCKRLKSLCPEHRKLGDEQHLKV 346

Query: 564 CGCP LVRDVFELTGDF CRLPKRQCNRHYCWEKLRRAEVDLERVR 607

CG P V ++ E+ F CR K C++H+ W R ++LE+

Sbjct: 347 CGYPKKWEDGMIETAKTVSELIEMEDPFGEEGCRTKKDACHKHHKWIPSLRGTIELEQAC 406

Query: 608 VWYKLDELFEQ--ERNVRTAMTNRAGLLALMLHQTIQHDPLTTDLRSΞA 654

++ K+ EL + + N T A L++M+H+ + + LR+ A Sbjct: 407 LFQKMYELCHEMHKLNAHAEWTTNA—LΞIMMHKQPSTEKCSFFLRNFA 453

Score = 53 (8.0 bits), Expect = 8.8e-27, Sum P(2) = 3e-27 Identities = 24/100 (24%), Positives = 41/100 (41%)

Query: 169 CGECEACRRTEDCGHCDFCR DMKK-FGGPNKIRQKCRLRQCQLRARESYKYFPSS 222

C C C ++CG C CR DM+K F +K + RQ + + + Sbjct: 17 CMNCIRCNDEKNCGTCWPCRNGKTCDMRKCFSAKRLYNEKVK-RQTDENLK-AIMAKTAQ 74

Query: 223 LSPVTPSESLPRPRRPLPTQQQPQPSQKLGRIR-EDEGAVASS 264

+ + P P+ +QQ + +K GR + G A++ Sbjct: 75 REAAHQAATTTAPSAPVVIEQQVE-KKKRGRKKGSGNGGAAAA 116

Score = 48 (7.2 bits), Expect = 2.9e-26, Sum P(2) = 2.9e-26 Identities = 13/39 (33%), Positives = 19/39 (48%)

Query: 179 EDCGHCDFCRDMKKFGG—PNKIRQKCRLRQCQLRARESY 216

E C +C C D K G P + + C +R+C A+ Y Sbjct: 15 ERCMNCIRCNDEKNCGTCWPCRNGKTCDMRKC-FSAKRLY 53

Pedant information for DKFZphtes3_4f17, frame 3

Report for DKFZphtes3_4f17.3

[LENGTH] 656 [MW] 75711.71 [pi] 8.61 [HOMOL] TREMBL :CEF52B11_4 gene: "F52B11.1"; Caenorhabditis elegans cosmid F52B11 3e-25

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YPL138c] 3e-10

[FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YNL097c] 2e-04

[PROSITE] MYRISTYL 6

[PROSITE] AMIDATION 2

[PROSITE] CK2 PHOSPHO SITE 8

[PROSITE] TYR PHOSPHO SITE 3

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC PHOSPHO SITE 9

[KW] All Alpha

[KW] LOW COMPLEXITY 18.75 %

[KW] COILED COIL 4.57 %

SEQ MEGDGSDPEPPDAGEDSKSENGENAPIYCICRKPDINCFMIGCDNCNEWFHGDCIRITEK SEG PRD cccccccccccccccccccccccccceeeeeeccccceeeeecccccccccccchhhhhh COILS

SEQ MAKAIREWYCRECREKDPKLEIRYRHKKSRERDGNERDSSEPRDEGGGRKRPVPDPDLQR SEG PRD hhhhhhhhhhhccccccccchhhhhhhhhccccccccccccccccccccccccccccccc COILS

SEQ RAGSGTGVGAMLARGSASPHKSSPQPLVATPSQHHQQQQQQIKRSARMCGECEACRRTED SEG xxxxxxxxx PRD cccccccceeeecccccccccccccccccchhhhhhhhhhhhhhhhhhcccccccccccc COILS SEQ CGHCDFCRDMKKFGGPNKIRQKCRLRQCQLRARESYKYFPSSLSPVTPSESLPRPRRPLP SEG xxxxxxxxxxxxxx xxxxxxxxxxxxxx PRD cccccccccccccccccchhhhhhhhhhhhhhhhhhcccccccccccccccccccccccc COILS

SEQ TQQQPQPSQKLGRIREDEGAVAΞSTVKEPPEATATPEPLSDEDLPLDPDLYQDFCAGAFD SEG xxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc COILS

SEQ DHGLPWMSDTEESPFLDPALRKRAVKVKHVKRREKKSEKKKEERYKRHRQKQKHKDKWKH SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchh COILS

SEQ PERADAKDPASLPQCLGPGCVRPAQPSSKYCSDDCGMKLAANRIYEILPQRIQQWQQSPC SEG PRD hhhhhccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhccch COILS

SEQ IAEEHGKKLLERIRREQQSARTRLQEMERRFHELEAIILRAKQQAVREDEESNEGDSDDT SEG xxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccc COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ DLQIFCVΞCGHPINPRVALRHMERCYAKYESQTSFGSMYPTRIEGATRLFCDVYNPQSKT SEG x PRD ceeeeeeeccccccccchhhhhhhhhhhhhhcccccccccccccccceeeeeeccccccc COILS

SEQ YCKRLQVLCPEHSRDPKVPADEVCGCPLVRDVFELTGDFCRLPKRQCNRHYCWEKLRRAE SEG PRD cchhhhhhhccccccccccceeeeccccchhhhhccccccccccccccchhhhhhhhhhh COILS

SEQ VDLERVRVWYKLDELFEQERNVRTAMTNRAGLLALMLHQTIQHDPLTTDLRSSADR SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccccc COILS

Prosite for DKFZphtes3_4f17.3

PS00002 124-M28 GLYCOSAMINOGLYCAN PDOC00002 PS00005 58->61 PKC_PHOSPHO_SITE PDOC00005 PS00005 165->168 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 215->218 PKC_PHOΞPHO_SITE PDOC00005 PS00005 248->251 PKC_PHOSPHO_SITE PDOC00005 PS00005 265->268 PKC_PHOSPHO_SITE PDOC00005 PS00005 337->340 PKC_PHOSPHO_SITE PDOC00005 PS00005 387->390 PKC_PHOSPHO_SITE PDOC00005 PS00005 439->442 PKC_PHOSPHO_SITE PDOC00005 PS00005 627->630 PKC_PHOSPHO_SITE PDOC00005 PS00006 6->10 CK2_PHOSPHO_SITE PDOC00006 PS00006 17->21 CK2_PHOSPHO_SITE PDOC00006 PS00006 227->231 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 265->269 CK2_PHOSPHO_SITE PDOC00006 PS00006 280->284 CK2_PHOSPHO_SITE PDOC00006 PS00006 308->312 CK2_PHOSPHO_SITE PDOC00006 PS00006 521->525 CK2_PHOSPHO_SITE PDOC00006 PS00006 652->656 CK2_PHOSPHO_SITE PDOC00006 PS00007 339->346 TYR_PHOSPHO_SITE PDOC00007 PS00007 500->507 TYR_PHOSPHO_SITE PDOC00007 PS00007 211->219 TYR_PHOSPHO_SITE PDOC00007 PS00008 42->48 MYRISTYL PDOC00008 PS00008 123->129 MYRISTYL PDOC00008 PS00008 125->131 MYRISTYL PDOC00008 PS00008 129->135 MYRISTYL PDOC00008 PS00008 259->265 MYRISTYL PDOC00008 PS00008 396->402 MYRISTYL PDOC00008 PS00009 107->111 AMIDATION PDOC00009 PS00009 425->429 AMIDATION PDOC00009

(No Pfam data available for DKFZphtes3 4fl7.3) DKFZphtes3_4f5

group: signal transduction

DKFZphtes3 4f5.3encodes a novel 790 amino acid protein similar to beta-transducins .

The protein contains 3 WD-40 repeats, which are typical for the beta-transducm subunit of G- proteins. The beta subunits seem to be required for the replacement of GDP by GTP as well as for membrane anchoring and receptor recognition. In addition, a Cytochrome C family heme- binding site signature is present. The protein is larger (790 ammo acids (than the usual eukaryotic G-beta transduc s (about 340 amino acids) .

The new protein can find application in modulating/blocking G-protem-dependent pathways. similarity to S. pombe "beta-transducm" complete cDNA, EST hits complete eds, on genomic level encoded by HS313D11, at least 7 exons these exons match only partialy with the predicted transcripts in HS313D11

Sequenced by AGOWA

Locus: /map="16pl3.3"

Insert length: 3166 bp

No poly A stretch found, no polyadenylation signal found

1 GGCGGCTTCC GGCGCGGCGG TTCCGGACAA CCGTGCGCTT TTAGTAAAAG 51 ATTGGGGTTC GCGCGGGGGA GAAGGGCTGC CCCGGGCCCT CTGGTTCTCG

101 TCCCGCAGCG TCCGCTCCCC CGCGCCACTG CGCCGCTCCC AGGAACCCTG

151 TACTCCGGGG TCGCCGGCTT CTCTCCTGCC TCCGGTCCCG CCAGACACCT

201 CGAGCTCCTT AAGTAGCTCG GTCCTTGACG TCCCTCTGGG CCCTTCCCGC

251 GTCTATCGCC TGAGTCCCCG GGCCCCTCTA GCCCTCTGTT CCCTCCCCTC

301 TTTTGTTCCT CCCTAGAGCC CCGCCGCCCT CAGGGCTGAC AGTGTGGACG

351 GCGGGAGTCT CCTCGCTCCC CTGCTGGGAT TGACTGACCG AGCGTTTAGT

401 GACTGCCCAG ATCTGGCTGA TGGGGGTACC GAGAGGTGGC CTGGGCCGGG

451 AATGTCCAGC TAGAGTCTTC CGTGGAAGTC AGACATGAAA CTGACAGGCC

501 TAAGGGAAGC TAGGAAGTCC CCTCACCGCT CAGCCAGGGT GATGGGCTGG

551 ACTGACAGAC TCCAGTGAAT TTGAGCTTGC CTGTCAGGCT GATTGGCTGA

601 TAGACAGCCC TGGATTGGCT CACTAAGACT GACCAGCCCG GGACCAAGCA

651 GTTCTGGGGT CCCAACCTGG GTGGAAGGTC TGAACTGATG ACCCACCCAG

701 GCTGACCAGG CCAGCCCACC TCACTGACCT CCTGACCCCT GACCTCATCA

751 CCTGTGCAGC CATGGAGAAG ATGTCCCGTG TGACCACAGC CCTGGGTGGC

801 AGCGTGCTGA CAGGCCGCAC CATGCACTGC CACCTGGATG CTCCCGCCAA

851 TGCCATCAGT GTGTGCCGCG ACGCAGCCCA GGTGGTCGTG GCAGGCCGTA

901 GCATCTTCAA GATCTATGCC ATCGAGGAGG AACAGTTCGT GGAAAAGCTG

951 AACCTGCGTG TGGGGCGCAA GCCTTCGCTT AACCTGAGCT GTGCTGACGT 1001 GGTCTGGCAC CAGATGGATG AGAACCTGCT GGCCACAGCA GCCACCAATG 1051 GCGTGGTGGT CACGTGGAAC CTGGGCCGGC CATCCCGCAA CAAGCAGGAC 1101 CAGCTGTTCA CAGAACACAA GCGCACGGTA AACAAAGTCT GCTTCCACCC 1151 CACCGAAGCC CACGTGCTGC TCAGTGGCTC CCAGGATGGC TTCATGAAGT 1201 GCTTTGACCT CCGCAGAAAG GACTCTGTCA GCACCTTCTC GGGCCAGTCG 1251 GAGAGCGTGC GGGACGTGCA GTTCAGTATC CGGGACTACT TCACCTTCGC 1301 CTCCACCTTT GAGAACGGCA ATGTGCAGCT CTGGGACATC CGGCGTCCCG 1351 ACCGGTGCGA GAGGATGTTC ACAGCCCACA ACGGACCCGT CTTCTGCTGC 1401 GACTGGCACC CCGAGGACAG GGGCTGGTTG GCCACTGGAG GGCGCGACAA 1451 GATGGTGAAG GTCTGGGACA TGACCACGCA CCGTGCCAAG GAGATGCACT 1501 GTGTGCAGAC CATCGCCTCG GTGGCCCGTG TGAAGTGGCG GCCAGAGTGC 1551 CGCCACCACC TGGCCACGTG CTCCATGATG GTGGACCACA ACATCTATGT 1601 TTGGGACGTG CGCCGGCCCT TCGTGCCAGC TGCCATGTTT GAGGAACACC 1651 GAGACGTCAC CACGGGAATT GCCTGGCGCC ACCCCCACGA CCCCTCCTTC 1701 CTGCTGTCTG GCTCCAAGGA CAGCTCGCTG TGCCAGCACC TGTTCCGCGA 1751 CGCCAGCCAG CCCGTCGAGC GCGCCAACCC TGAGGGCCTC TGCTACGGCC 1801 TCTTCGGGGA CCTGGCCTTC GCCGCCAAGG AGAGCCTCGT GGCTGCCGAG 1851 TCGGGGCGCA AGCCCTACAC TGGCGACCGG CGCCACCCCA TCTTCTTTAA 1901 GCGCAAGCTG GACCCTGCCG AGCCCTTCGC AGGCCTCGCC TCCAGTGCCC 1951 TCAGTGTCTT TGAGACGGAG CCAGGTGGCG GCGGCATGCG CTGGTTTGTG 2001 GACACAGCTG AGCGTTATGC GCTGGCTGGC CGGCCACTGG CCGAGCTCTG 2051 TGACCACAAC GCAAAGGTGG CTCGAGAGCT TGGCCGCAAC CAGGTGGCGC 2101 AAACGTGGAC CATGCTGCGG ATCATCTACT GCAGCCCTGG CCTAGTGCCC 2151 ACTGCAAACC TCAACCACAG TGTGGGCAAG GGTGGCTCCT GTGGCCTCCC 2201 GCTCATGAAC AGTTTCAACC TGAAGGATAT GGCCCCAGGG TTGGGCAGTG 2251 AGACGCGGCT GGACCGCAGC AAAGGAGATG CACGGAGCGA CACAGTTCTG 2301 CTCGACTCCT CGGCCACACT CATCACCAAT GAGGATAACG AGGAAACCGA 2351 GGGCAGCGAC GTACCTGCCG ACTACCTGCT GGGTGACGTG GAAGGTGAGG 2401 AGGACGAGCT GTACCTGCTG GATCCGGAAC ACGCGCACCC CGAGGACCCT

2451 GAGTGCGTGC TGCCGCAGGA GGCCTTTCCG CTGCGCCACG AGATCGTGGA

2501 CACGCCTCCC GGACCCGAGC ACCTGCAGGA CAAGGCCGAC TCCCCGCACG

2551 TGAGCGGCAG CGAGGCGGAT GTGGCCTCCC TGGCCCCCGT GGACTCCTCC

2601 TTCTCGCTCC TGTCTGTCTC ACACGCGCTC TACGACAGCC GCCTGCCGCC

2651 CGACTTCTTC GGCGTGCTGG TGCGCGACAT GCTGCACTTC TACGCTGAGC

2701 AGGGCGACGT GCAGATGGCT GTGTCTGTGC TCATCGTCCT GGGTGAACGG

2751 GTGCGCAAGG ACATCGACGA GCAGACCCAG GAGCACTGGT ACACTTCCTA

2801 CATCGACCTG CTGCAGCGCT TCCGCCTCTG GAACGTGTCC AACGAGGTGG

2851 TCAAGCTGAG CACCAGCCGC GCCGTCAGCT GCCTCAACCA GGCCTCCACC

2901 ACCCTGCACG TCAACTGCAG CCACTGCAAG CGGCCCATGA GCAGCCGGGG

2951 CTGGGTCTGC GACAGGTGCC ACCGCTGCGC CAGCATGTGT GCCGTCTGCC

3001 ACCACGTAGT CAAGGGTCTC TTCGTGTGGT GCCAGGGCTG CAGCCACGGC

3051 GGCCACCTGC AGCACATCAT GAAGTGGCTG GAAGGCAGCT CCCACTGTCC

3101 CGCAGGCTGC GGCCACCTCT GCGAGTACTC CTGACGGGGC ATCTGCTGGG

3151 CTTGCCCGGG CGGCCG

BLAST Results

Entry HS313D11 from database EMBL:

Human DNA sequence from cosmid 313D11 from a contig on the short arm of chromosome 16. Contains ESTs, STS and CpG islands.

Score = 6238, P = O.Oe+00, identities = 1318/1391

Medline entries

No Medlme entry

Peptide information for frame 3

ORF from 762 bp to 3131 bp; peptide length: 790 Category: similarity to known protein

1 MEKMSRVTTA LGGSVLTGRT MHCHLDAPAN AISVCRDAAQ VVVAGRSIFK 51 IYAIEEEQFV EKLNLRVGRK PSLNLSCADV VWHQMDENLL ATAATNGVVV 101 TWNLGRPSRN KQDQLFTEHK RTVNKVCFHP TEAHVLLSGS QDGFMKCFDL 151 RRKDSVSTFS GQSESVRDVQ FSIRDYFTFA STFENGNVQL WDIRRPDRCE 201 RMFTAHNGPV FCCDWHPEDR GWLATGGRDK MVKVWDMTTH RAKEMHCVQT 251 IAΞVARVKWR PECRHHLATC SMMVDHNIYV WDVRRPFVPA AMFEEHRDVT 301 TGIAWRHPHD PSFLLSGSKD ΞSLCQHLFRD ASQPVERANP EGLCYGLFGD 351 LAFAAKESLV AAESGRKPYT GDRRHPIFFK RKLDPAEPFA GLASSALSVF 401 ETEPGGGGMR WFVDTAERYA LAGRPLAELC DHNAKVAREL GRNQVAQTWT 451 MLRIIYCSPG LVPTANLNHS VGKGGSCGLP LMNSFNLKDM APGLGSETRL 501 DRSKGDARSD TVLLDSSATL ITNEDNEETE GSDVPADYLL GDVEGEEDEL 551 YLLDPEHAHP EDPECVLPQE AFPLRHEIVD TPPGPEHLQD KADSPHVSGS 601 EADVASLAPV DSSFSLLSVS HALYDSRLPP DFFGVLVRDM LHFYAEQGDV 651 QMAVSVLIVL GERVRKDIDE QTQEHWYTSY IDLLQRFRLW NVSNEVVKLS 701 TSRAVSCLNQ ASTTLHVNCS HCKRPMSSRG WVCDRCHRCA SMCAVCHHVV 751 KGLFVWCQGC ΞHGGHLQHIM KWLEGSSHCP AGCGHLCEYS

BLASTP hits

Entry YDSB_SCHPO from database SWISSPROT:

HYPOTHETICAL 93.2 KD TRP-ASP REPEATS CONTAINING PROTEIN C4F8.11 IN

CHROMOSOME I. >TREMBL : SPAC4F8_11 gene: "SPAC4F8.il"; product:

"beta-transducm"; S. pombe chromosome I cosmid c4F8.

Score = 404, P = 3.0e-42, identities = 169/639, positives = 278/639

Entry PEX7_HUMAN from database SWISSPROT:

PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR (PTS2 RECEPTOR) (PEROXIN-7) .

>TREMBL:HSU76560_1 gene: "Pex7"; product: "peroxisome targeting signal

2 receptor"; Human peroxisome targeting signal 2 receptor (Pex7) mRNA, complete eds. >TREMBL:HSU88871_1 gene: "HsPEX7"; product: "HsPex7p";

Human HsPex7p (HsPEX7) mRNA, complete eds.

Score = 220, P = l.le-15, identities = 62/244, positives = 107/244

Entry PEX7_MOUSE from database SWISSPROT:

PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR (PTS2 RECEPTOR) (PEROXIN-7) .

>TREMBL:MMU69171_1 product: "peroxisomal PTΞ2 receptor"; Mus musculus peroxisomal PTS2 receptor mRNA, complete eds.

Score = 214, P = 5.3e-15, identities = 60/240, positives = 106/240 Entry ATAC2294_7 from database TREMBL: gene: "F11P17.7"; Arabidopsis thaliana chromosome I BAC F11P17 genomic sequence, complete sequence.

Score = 232, P = 3.4e-14, identities = 68/260, positives = 120/260

Entry S66835 from database PIR: probable membrane protein YOL138c - yeast (Saccharomyces cerevisiae)

>TREMBL:SCYOL138C_l S. cerevisiae chromosome XV reading frame ORF

YOL138C

Score = 136, P = 2.5e-13, identities = 24/77, positives = 44/77

Alert BLASTP hits for DKFZphtes3_4f5, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphtes3_4f5, frame 3

Report for DKFZphtes3_4f5.3

[LENGTH] 790 [MW] 88207.10 [pi] 6.05 [HOMOL] SWISSPROT :YDSB_SCHPO HYPOTHETICAL 93.2 KD TRP-ASP REPEATS CONTAINING PROTEIN C4F8.11 IN CHROMOSOME I. 9e-44 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YOL138c] 5e-16 [FUNCAT] 10.04.09 regulation of g-protein activity [S. cerevisiae, YBR195c] 3e-ll [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YBR195c] 3e-ll [FUNCAT] 03.16 dna synthesis and replication [S. cerevisiae, YBR195C] 3e-ll [FUNCAT] 09.13 biogenesis of chromosome structure [S. cerevisiae, YBR195c] 3e-ll [FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YBR195c] 3e-ll [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YCR072c beta-transducin family] 3e-10 [FUNCAT] 04.05.01.01 general transcription activities [S. cerevisiae, YBR198c TAF90 - TFIID subunit] 9e-09 [FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YLLOllw] le-07 [FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YDL195W] 2e 07 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL195w] 2e-07 [FUNCAT] 30.19 peroxisomal organization [S. cerevisiae, YDR142C] 4e-07 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YDR142c] 4e-07 [FUNCAT] 08.10 peroxisomal transport [S. cerevisiae, YDR142c] 4e-07 [FUNCAT] 08.01 nuclear transport [S. cerevisiae, YER107c] 4e-07 [FUNCAT] 04.07 rna transport [S. cerevisiae, YER107c] 4e-07 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YER107c] 4e-07 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YGL003c] 5e-07 [FUNCAT] 06.13 proteolysis [S. cerevisiae, YGL003c] 5e-07 [ FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YCR084C] 8e-07 [FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YPR178w] le-06 [FUNCAT] 03.13 meiosis [S. cerevisiae, YLR129w] 3e-06 [FUNCAT] 03.25 cytokinesis [S. cerevisiae, YCR057c] le-05 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YCR057c] le-05 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YEL056w] 2e-04 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YOR272w] 6e-04 [SCOP] dlgotb_ 2.46.3.1.1 betal-subunit of the signal-transducmg 5e-06 [PIRKW] duplication 7e-10 [PIRKW] signal transduction 7e-08 [PIRKW] peroxisome 9e-06 [PIRKW] heterotrimer 7e-08 [PIRKW] GTP binding 7e-08 [PIRKW] peroxisome biogenesis 9e-06 [PIRKW] transmembrane protein le-14 [SUPFAM] MSI1 protein 7e-10 [SUPFAM] WD repeat homology le-14 [SUPFAM] GTP-binding regulatory protein beta chain 7e-08 [SUPFAM] PRL1 protein 3e-08 [SUPFAM] coatomer complex beta' chain le-06 [PROSITE] CYTOCHROME_C 1 [PROSITE] WD_REPEATS 3 [PROSITE] MYRISTYL 10 [PROSITE] AMIDATION 2 [PROSITE] CAMP_PHOSPHO_SI E 2 [PROSITE] CK2 PHOSPHO SITE 11 [PROSITE] TYR_PHOSPHO_SITE 1

[PROSITE] PKC_PHOSPHO_SITE 7

[PROSITE] ASN_GLYCOSYLATION 4

[PFAM] WD domain, G-beta repeats

[KW] All_Beta

[KW] 3D

[KW] LOW COMPLEXITY 2.28 %

SEQ MEKMSRVTTALGGSVLTGRTMHCHLDAPANAISVCRDAAQVVVAGRSIFKIYAIEEEQFV SEG IgotB

SEQ EKLNLRVGRKPSLNLSCADVVWHQMDENLLATAATNGVVVTWNLGRPSRNKQDQLFTEHK SEG IgotB TTCEEEEEETTTEEEEEET-TTTCEEE—EEECCC

SEQ RTVNKVCFHPTEAHVLLSGSQDGFMKCFDLRRKDSVSTFSGQSESVRDVQFSIRDYFTFA SEG IgotB CCEEEEEEETT-TCEEEEEETTTEEEEEETTTTEEEEEECBTTCCEEEEEETTTTTEEEE

SEQ STFENGNVQLWDIRRPDRCERMFTAHNGPVFCCDWHPEDRGWLATGGRDKMVKVWDMTTH SEG IgotB E-ETTTEEEEEETTTTEEEE-EEECCCCCEEEEEE-TTTTCCEEEEETTTEEEEEC....

SEQ RAKEMHCVQTIASVARVKWRPECRHHLATCSMMVDHNIYVWDVRRPFVPAAMFEEHRDVT SEG IgotB

SEQ TGIAWRHPHDPSFLLSGSKDSSLCQHLFRDASQPVERANPEGLCYGLFGDLAFAAKESLV SEG IgotB

SEQ AAESGRKPYTGDRRHPIFFKRKLDPAEPFAGLASSALSVFETEPGGGGMRWFVDTAERYA SEG IgotB

SEQ LAGRPLAELCDHNAKVARELGRNQVAQTWTMLRIIYCSPGLVPTANLNHSVGKGGSCGLP SEG IgotB

SEQ LMNSFNLKDMAPGLGSETRLDRSKGDARSDTVLLDSSATLITNEDNEETEGSDVPADYLL SEG xxxx IgotB

SEQ GDVEGEEDELYLLDPEHAHPEDPECVLPQEAFPLRHEIVDTPPGPEHLQDKADSPHVSGS SEG xxxxxxxxxxxxx IgotB

SEQ EADVASLAPVDSSFSLLSVSHALYDSRLPPDFFGVLVRDMLHFYAEQGDVQMAVSVLIVL SEG IgotB

SEQ GERVRKDIDEQTQEHWYTSYIDLLQRFRLWNVSNEVVKLSTSRAVSCLNQASTTLHVNCS SEG IgotB

SEQ HCKRPMSSRGWVCDRCHRCASMCAVCHHVVKGLFVWCQGCSHGGHLQHIMKWLEGSSHCP SEG IgotB

SEQ AGCGHLCEYS SEG IgotB

Prosite for DKFZphtes3_4f5.3

PS00001 74->78 ASN_GLYCOSYLATION PDOC00001 PS00001 468->472 ASN_GLYCOSYLATION PDOC00001 PS00001 691->695 ASN_GLYCOΞYLATION PDOC00001 PS00001 718->722 ASN_GLYCOSYLATION PDOC00001 PS00004 69->73 CAMP_PHOSPHO_SITE PDOC00004 PS00004 152->156 CAMP_PHOΞPHO_SITE PDOC00004 PΞ00005 17->20 PKC_PHOSPHO_SITE PDOC00005 PS00005 165->168 PKC_PHOSPHO_SITE PDOC00005 PS00005 172->175 PKC_PHOSPHO_SITE PDOC00005 PS00005 239->242 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 364->367 PKC_PHOSPHO_SITE PDOC00005 PS00005 701->704 PKC PHOSPHO SITE PDOC00005

DKFZphtes3_4h6 group: intracellular transport/trafficking

DKFZphtes3_4h6 encodes a novel 622 amino acid protein with strong similarity to the kinesm light chain.

Kmesin is a microtubule-based motor protein that pulls vesicles or organelles towards the plus end of microtubules. Structural changes in the protein that drive motility are coupled to ATP binding and hydrolysis. The novel protein is similar to kmesin light chain, which is part of the functional kmesin holoenzyme tetrameric protein. The light chain has been proposed to function in coupling of cargo to the heavy chain or in the modulation of the ATPase activity of the heavy chain. The novel protein contains two kinesm light chain repeats and one RGD cell-attachment site.

The novel kinesin protein can find application in modulating the function of kinesm and modulating intracellular transport via/on microtubules. strong similarity to Kinesm light chain complete cDNA, complete eds, start at 150, EST hits (few)

Sequenced by AGOWA

Locus : unknown

Insert length: 2992 bp

Poly A stretch at pos. 2914, polyadenylation signal at pos. 2893

1 GGCGGGATGG AGGCGGCGGG ACCGGCTCGC GGGTGCGGGT CCGGGTGAAG 51 CGGGAGGCAG CCAGAGTCGG AGCCGGGCCC GAGCACCAGG CGCAGGCCCG

101 GCGCCCGCCT GCCCGCACCC TCGTCCTCAC AGACGCCACA GCCATGGCCA

151 TGATGGTGTT TCCGCGGGAG GAGAAGCTGA GCCAGGATGA GATCGTGCTG

201 GGCACCAAGG CTGTCATCCA GGGACTGGAG ACTCTGCGTG GGGAGCATCG

251 TGCCCTGCTG GCTCCTCTGG TTGCACCTGA GGCCGGCGAA GCCGAGCCTG

301 GCTCGCAGGA GCGCTGCATC CTCCTGCGTC GCTCCCTGGA AGCCATTGAG

351 CTTGGGCTGG GGGAGGCCCA GGTGATCTTG GCATTGTCGA GCCACCTGGG

401 GGCTGTAGAA TCAGAGAAGC AGAAGCTGCG GGCGCAGGTG CGGCGTCTGG

451 TGCAGGAGAA CCAGTGGCTG CGTGAGGAGC TGGCGGGGAC ACAGCAGAAG

501 CTGCAGCGCA GTGAGCAGGC CGTGGCCCAG CTCGAGGAGG AGAAGCAGCA

551 CTTGCTGTTC ATGAGCCAGA TCCGCAAGTT GGATGAAGAC GCCTCCCCTA

601 ACGAGGAGAA GGGGGACGTC CCCAAAGACA CACTGGATGA CCTGTTCCCC

651 AATGAGGATG AGCAGAGCCC AGCCCCTAGC CCAGGAGGAG GGGATGTGTC

701 TGGTCAGCAT GGGGGCTACG AGATCCCGGC CCGGCTCCGC ACCCTGCACA

751 ACCTGGTGAT CCAATACGCC TCACAGGGCC GCTACGAGGT AGCTGTGCCA

801 CTCTGCAAGC AGGCACTCGA AGACCTGGAG AAGACGTCAG GCCACGACCA

851 CCCTGACGTT GCCACCATGC TGAACATCCT GGCACTGGTC TATCGGGATC

901 AGAACAAGTA CAAGGAGGCT GCCCACCTGC TCAATGATGC TCTGGCCATC

951 CGGGAGAAAA CACTGGGCAA GGACCACCCA GCCGTGGCTG CGACACTAAA 1001 CAACCTGGCA GTCCTGTATG GCAAGAGGGG CAAGTACAAG GAGGCTGAGC 1051 CATTGTGCAA GCGGGCACTG GAGATCCGGG AGAAGGTCCT GGGCAAGTTT 1101 CACCCAGATG TGGCCAAGCA GCTCAGCAAC CTGGCCCTGC TGTGCCAGAA 1151 CCAGGGCAAA GCTGAGGAGG TGGAATATTA CTATCGGCGG GCACTGGAGA 1201 TCTATGCTAC ACGCCTCGGG CCCGATGACC CCAATGTGGC CAAGACCAAG 1251 AACAACCTGG CTTCCTGCTA CCTGAAGCAG GGCAAGTACC AGGATGCGGA 1301 GACCTTGTAC AAGGAGATCC TCACCCGCGC TCATGAGAAA GAGTTTGGCT 1351 CTGTCAATGG GGACAACAAG CCCATCTGGA TGCACGCAGA GGAGCGGGAG 1401 GAAAGCAAGG ATAAGCGCCG GGACAGCGCC CCCTATGGGG AATACGGCAG 1451 CTGGTACAAG GCCTGTAAAG TAGACAGCCC CACAGTCAAC ACCACCCTGC 1501 GCAGCTTGGG GGCCCTATAC CGGCGCCAGG GCAAGCTGGA AGCCGCGCAC 1551 ACACTAGAGG ACTGTGCCAG CCGTAACCGC AAGCAGGGTT TGGACCCCGC 1601 AAGCCAGACC AAGGTGGTAG AACTGCTGAA AGATGGCAGT GGCAGGCGGG 1651 GAGACCGCCG CAGCAGCCGA GACATGGCTG GGGGTGCCGG GCCTCGGTCT 1701 GAGTCTGACC TCGAGGACGT GGGACCTACA GCTGAGTGGA ATGGGGATGG 1751 CAGTGGCTCC TTGAGGCGCA GCGGTTCCTT TGGGAAACTC CGGGATGCCC 1801 TGAGGCGCAG CAGTGAGATG CTGGTAAAGA AGCTGCAGGG GGGCACCCCC 1851 CAGGAGCCCC CTAACCCCAG GATGAAGCGG GCCAGTTCCC TCAACTTCCT 1901 CAACAAGAGC GTGGAAGAGC CGACCCAGCC TGGAGGCACA GGTCTCTCTG 1951 ACAGCCGCAC TCTCAGCTCC AGCTCCATGG ACCTCTCCCG ACGAAGCTCC 2001 CTGGTGGGCT AATGCTGAAG GGGCAGCCAG TCACCAGAGC GCCCACCTGG 2051 CACACCCCCC TCACCCCAGC CCTGCGCATG GGCCTGCTGC TTGTCCCGCC 2101 TGTCTCTCCC ACAGCCCCTG TCTTTTCTGT TCAATCTCAG GGTAACCTTC 2151 TCCCTTGTCA TCTCAGCCTG AGCCCTGGAG GCTGGGCCTG CCCACTCCAG 2201 CTCCATCCCT TATTTATTCC TTCCAGCAGG GCCCTCTTCC CTAGGTTCGG 2251 GCCAGCAGGA GGTGCCGGCT GGAGTCTCCA CCATAGACTC AGTGGCCTGG 2301 CCTCCCCAGA CCCCAGAGCC AAGAACACTA AGCACTCGCC GGCCCTTCGG 2351 CACCCTCGCC CTCCCTCCCG ACTCAACCCG GCCGTTGCTT CTGTATATAG 2401 AGAAATAAGT TATTGGCCGC GCGCCTCCCT TCAGTCCACG GTACTACCCG 2451 GGCCTCCCCT CGTCCCTCTT CTAGTGGTAC CGCCCAGGCC TTAATCACCC

2501 CCATTCCGTG CGGTGGTATC TCCCAGGCTC TACATTCTCG GGAGCGGCGC

2551 CTCCCAAGGG GGTCCTGGGA CCTTCTCGCG CTCCTCCTGG CCTCTGAGGG

2601 ATGCGTCCTA CCCGCGCCAT CGCCCCGTGG CCCAGGACGG GGACCTCCCC

2651 TTAGTCCGTC CTCCCACCGC CGGGCCCTGC CCCGCATCCC GGCCTTATGC

2701 ACTGCCCCTC CCACCCGGCC CCGCCCAGGC ACGGCCGACC CCGCCCCGGG

2751 CACCGCCCAC CGAGCCATCC TGCCTCGCCT CCCCCCACGC CTGCAGCTTC

2801 TCGCGAGGGG CGGCGACGGT CCCCTGGTGG CAGGAGGGGC TCCCCCTGTT

2851 GCGGGTGAGG CGGCTGCTCT CTATTTTCAG ATGTTGCTGT AGAAATAAAG

2901 ACGGTTTAAA TCTGAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

2951 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AA

BLAST Results

No BLAST result

Medline entries

98288268:

Two kinesin light chain genes in mice. Identification and characterization of the encoded proteins.

Peptide information for frame 3

ORF from 144 bp to 2009 bp; peptide length: 622 Category: strong similarity to known protein Prosite motifs: RGD (502-505) KINESIN_LIGHT (223-265) KINESIN LIGHT (265-307)

1 MAMMVFPREE KLSQDEIVLG TKAVIQGLET LRGEHRALLA PLVAPEAGEA 51 EPGSQERCIL LRRSLEAIEL GLGEAQVILA LSSHLGAVES EKQKLRAQVR 101 RLVQENQWLR EELAGTQQKL QRSEQAVAQL EEEKQHLLFM SQIRKLDEDA 151 SPNEEKGDVP KDTLDDLFPN EDEQSPAPSP GGGDVSGQHG GYEIPARLRT 201 LHNLVIQYAS QGRYEVAVPL CKQALEDLEK TSGHDHPDVA TMLNILALVY 251 RDQNKYKEAA HLLNDALAIR EKTLGKDHPA VAATLNNLAV LYGKRGKYKE 301 AEPLCKRALE IREKVLGKFH PDVAKQLΞNL ALLCQNQGKA EEVEYYYRRA 351 LEIYATRLGP DDPNVAKTKN NLASCYLKQG KYQDAETLYK EILTRAHEKE 401 FGSVNGDNKP IWMHAEEREE SKDKRRDSAP YGEYGSWYKA CKVDSPTVNT 451 TLRSLGALYR RQGKLEAAHT LEDCAΞRNRK QGLDPASQTK VVELLKDGSG 501 RRGDRRSSRD MAGGAGPRSE SDLEDVGPTA EWNGDGSGSL RRSGSFGKLR 551 DALRRSSEML VKKLQGGTPQ EPPNPRMKRA SSLNFLNKSV EEPTQPGGTG 601 LSDSRTLΞSS SMDLSRRSSL VG

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_4h6, frame 3

TREMBL:AF055666_1 gene: "Klc2"; product: "kinesin light chain 2"; Mus musculus kmesin light chain 2 (Klc2) mRNA, complete eds., N = 1, Score = 2824, P = 4e-294

PIR: 153013 kinesin light chain - human, N = 1, Score = 1927, P = 4.5e-199

PIR:C41539 kinesin light chain C - rat, N = 1, Score = 1919, P = 3.2e-198

SWISSPROT :KNLC_RAT KINESIN LIGHT CHAIN (KLC) . , N = 1, Score = 1919, P = 3.2e-198

>TREMBL:AF055666_1 gene: "Klc2"; product: "kinesin light chain 2"; Mus musculus k esin light chain 2 (Klc2) mRNA, complete eds. Length = 599

HSPs: Score = 2824 (423.7 bits), Expect = 4.0e-294, P = 4.0e-294 Identities = 558/598 (93%), Positives = 572/598 (95%)

Query: 1 MAMMVFPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLVAPEAGEAEPGSQERCIL 60

MA MV PREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPL + EAGEAEPGSQERC+L Sbjct: 1 MATMVLPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLASHEAGEAEPGSQERCLL 60

Query: 61 LRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKL 120

LRRSLEAIELGLGEAQVILALSSHLGAVEΞEKQKLRAQVRRLVQENQWLREELAGTQQKL Sbjct: 61 LRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKL 120

Query: 121 QRSEQAVAQLEEEKQHLLFMSQIRKLDEDASPNEEKGDVPKDTLDDLFPNEDEQSPAPSP 180

QRSEQAVAQLEEEKQHLLFMSQIRKLDE P EEKGDVPKD+LDDLFPNEDEQSPAPSP Sbjct: 121 QRSEQAVAQLEEEKQHLLFMSQIRKLDE-MLPQEEKGDVPKDSLDDLFPNEDEQSPAPSP 179

Query: 181 GGGDVSGQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVA 240

GGGDV+ QHGGYEI PARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVA Sbjct: 180 GGGDVAAQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVA 239

Query: 241 TMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKE 300

TMLNILALVYRDQNKYK+AAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKE Sbjct: 240 TMLNILALVYRDQNKYKDAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKE 299

Query: 301 AEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGP 360

AEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGP Sbjct: 300 AEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGP 359

Query: 361 DDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGDNKPIWMHAEEREE 420

DDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNG+NKPIWMHAEEREE Sbjct: 360 DDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGENKPIWMHAEEREE 419

Query: 421 SKDKRRDSAPYGEYGSWYKACKVDSPTVNTTLRSLGALYRRQGKLEAAHTLEDCASRNRK 480

SKDKRRD P EYGSWYKACKVDSPTVNTTLR+LGALYR +GKLEAAHTLEDCASR+RK Sbjct: 420 SKDKRRDRRPM-EYGSWYKACKVDSPTVNTTLRTLGALYRPEGKLEAAHTLEDCASRSRK 478

Query: 481 QGLDPASQTKVVELLKDGSGRRGDRRSSRDMAGGAGPRSESDLEDVGPTAEWNGDGSGSL 540

QGLDPASQTKVVELLKDGSGR G RR SRD+AG P+SESDLE+ GP AEW+GDGSGSL Sbjct: 479 QGLDPASQTKVVELLKDGSGR-GHRRGSRDVAG PQSESDLEESGPAAEWSGDGSGSL 534

Query: 541 RRSGSFGKLRDALRRSSEMLVKKLQGGTPQEPPNPRMKRASSLNFLNKSVEEPTQPGG 598

RRSGSFGKLRDALRRSSEMLV+KLQGG PQEP N RMKRASSLNFLNKSVEEP QPGG Sbjct: 535 RRSGSFGKLRDALRRSSEMLVRKLQGGGPQEP-NSRMKRASSLNFLNKSVEEPVQPGG 591

Pedant information for DKFZphtes3_4h6, frame 3

Report for DKFZphtes3_4h6.3

[LENGTH] 622

[MW] 68934.82

[pi] 6.72

[HOMOL] TREMBL :AF055666_1 gene: "Klc2"; product: "kmesin light chain 2" Mus musculus kmesin light chain 2 (Klc2) mRNA, complete eds. 0.0

[BLOCKS] BL00927C Trehalase proteins

[BLOCKS] BL01160I Kinesm light chain repeat proteins

[BLOCKS] BL01160H Kinesm light chain repeat proteins

[BLOCKS] BL01160G Kinesm light chain repeat proteins

[BLOCKS] BL01160F Kinesin light chain repeat proteins

[BLOCKS] BL01160E Kinesin light chain repeat proteins

[BLOCKS] BL01160D Kinesin light chain repeat proteins

[BLOCKS] BL01160C Kinesin light chain repeat proteins

[BLOCKS] BL01160B Kinesin light chain repeat proteins

[BLOCKS] BL01160A Kinesin light chain repeat proteins

[SUPFAM] tetratπcopeptide repeat homology le-07

[PROSITE] RGD 1

[PROSITE] MYRISTYL 8

[PROSITE] KINESIN_LIGHT 2

[PROSITE] AMIDATION 2

[PROSITE] CAMP_PHOSPHO_SITE 5

[PROSITE] CK2_PHOSPHO_ΞI E 11

[PROSITE] TYR_PHOSPHO_SITE 3

[PROSITE] PKC_PHOSPHO_SITE 7

[PROSITE] ASN ΞLYCOSYLATION 2

[PFAM] Kmesin light chain repeat

[KW] All_Alpha

[KW] LOW_COMPLEXITY 12.54 %

[KW] COILED COIL 4.98 % SEQ MAMMVFPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLVAPEAGEAEPGSQERCIL SEG PRD ccccchhhhhhhhhhhhhchhhhhhhhhhhhhhchhhhhhhhhhhhhcccccccchhhhh COILS

SEQ LRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKL SEG PRD hhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcchhhhhhhhhhhhhh COILS CCCCCCCCCCCC

SEQ QRSEQAVAQLEEEKQHLLFMSQIRKLDEDASPNEEKGDVPKDTLDDLFPNEDEQSPAPSP SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccccccccccccccccccccccccc COILS CCCCCCCCCCCCCCCCCCC

SEQ GGGDVSGQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVA SEG PRD cccccccccccccchhhhhhhhhhhhhhhccceeeeeehhhhhhhhhhhhhccccccchh COILS

SEQ TMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKE SEG xxxxxxxxxxxx PRD hhhhhhhhhhhhcchhhhhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhcccccchh COILS

SEQ AEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGP SEG PRD hhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhhccchhhhhhhhhhhhhhhhhhccc COILS

SEQ DDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGDNKPIWMHAEEREE SEG xxxxx PRD ccccccchhhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhccccccccchhhhhhhhhh COILS

SEQ ΞKDKRRDSAPYGEYGSWYKACKVDSPTVNTTLRSLGALYRRQGKLEAAHTLEDCASRNRK SEG xxxxxxxx PRD hhhhhccccccccccccceeeeccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS

SEQ QGLDPASQTKVVELLKDGSGRRGDRRSSRDMAGGAGPRSESDLEDVGPTAEWNGDGSGSL SEG xxxxxxxxxxxxxx xxxxx PRD hhccchhhhhhhhhhccccccccccccccccccccccccccccccceeeecccccccccc COILS

SEQ RRSGSFGKLRDALRRSSEMLVKKLQGGTPQEPPNPRMKRASSLNFLNKSVEEPTQPGGTG SEG xxxxxxxxxx xxxx PRD ccccccchhhhhhhhhhhhhhhhhhcccccccccchhhhhhhcccccccccccccccccc COILS

SEQ LSDSRTLSSSSMDLSRRSSLVG SEG xxxxxxxxxxxxxxxxxxxx.. PRD cccccccccccchhhhhhcccc COILS

Prosite for DKFZphtes3_4h6.3

PS00001 449->453 ASN_GLYCOSYLATION PDOC00001 PS00001 587->591 ASN_GLYCOSYLATION PDOC00001 PS00004 425->429 CAMP_PHOSPHO_SITE PDOC00004 PΞ00004 505->509 CAMP_PHOSPHO_ΞITE PDOC00004 PS00004 554->558 CAMP_PHOSPHO_SITE PDOC00004 PS00004 578->582 CAMP_PHOSPHO_SITE PDOC00004 PS00004 616->620 CAMP_PHOSPHO_SITE PDOC00004 PS00005 30->33 PKC_PHOSPHO_SITE PDOC00005 PS00005 90->93 PKC_PHOSPHO_SITE PDOC00005 PS00005 451->454 PKC_PHOSPHO_SITE PDOC00005 PS00005 499->502 PKC_PHOSPHO_SITE PDOC00005 PS00005 507->510 PKC_PHOSPHO_SITE PDOC00005 PS00005 539->542 PKC_PHOSPHO_SITE PDOC00005 PS00005 615->618 PKC_PHOSPHO_SITE PDOC00005 PS00006 13->17 CK2_PHOSPHO_SITE PDOC00006 PS00006 151->155 CK2_PHOSPHO_SITE PDOC00006 PS00006 163->167 CK2_PHOSPHO_SITE PDOC00006 PS00006 232->236 CK2_PHOSPHO_SITE PDOC00006 PS00006 470->474 CK2_PHOSPHO_SITE PDOC00006 PS00006 507->511 CK2_PHOSPHO_SITE PDOC00006 PS00006 519->523 CK2_PHOSPHO_SITE PDOC00006 PS00006 521->525 CK2 PHOSPHO SITE PDOC00006 PS00006 568->572 CK2_PHOSPHO_SITE PDOC00006 PS00006 589->593 CK2_PHOSPHO_SITE PDOC00006 PS00006 610->614 CK2_PHOSPHO_SITE PDOC00006 PS00007 339->346 TYR_PHOSPHO_SITE PDOC00007 PS00007 339->347 TYR_PHOSPHO_SITE PDOC00007 PS00007 424->432 TYR_PHOSPHO_SITE PDOC00007 PS00008 71->77 MYRISTYL PDOC00008 PS00008 86->92 MYRISTYL PDOC00008 PS00008 182->188 MYRISTYL PDOC00008 PS00008 187->193 MYRISTYL PDOC00008 PS00008 402->408 MYRISTYL PDOC00008 PS00008 482->488 MYRISTYL PDOC00008 PS00008 598->604 MYRISTYL PDOC00008 PS00008 600->606 MYRISTYL PDOC00008 PS00009 292->296 AMIDATION PDOC00009 PS00009 499->503 AMIDATION PDOC00009 PS00016 502->505 RGD PDOC00016 PS01160 223->265 KINESIN_LIGHT PDOC00893 PS01160 265->307 KINESIN LIGHT PDOC00893

Pfam for DKFZphtes3_4h6.3

HMM_NAME Kmesin light Cham repeat

HMM *RALEDREKtlGHDHPDVAtMLNNLALvCRNQNKYeEveNYYN* +ALED+EKT+GHDHPDVATMLN+LALV+R+QNKY+E++ ++N

Query 223 QALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKEAAHLLN 264

50.46 265 306 1 42 dkfzphtes3_4h6.3 strong similarity to Kinesm light chain

Alignment to HMM consensus : Query *RALEDREKtlGHDHPDVAtMLNNLALvCRNQNKYeEveNYYN* AL +REKTLG DHP VA LNNLA+++ ++KY+E+E + + dkfzphtes3 265 DALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCK 306

Query 348 1 42 dkfzphtes3_4h6.3 strong similarity to Kmesin light chain

Alignment to HMM consensus: HMM *RALEDREKtlGHDHPDVAtMLNNLALvCRNQNKYeEveNYYN* RALE+REK+LG HPDVA++L+NLAL+C+NQ+K EEVE YY+

Query 307 RALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYR 348

39.10 349 390 1 42 dkfzphtes3_4h6.3 strong similarity to Kinesin light chain

Alignment to HMM consensus : Query *RALEDREKtlGHDHPDVAtMLNNLALvCRNQNKYeEveNYYN* RALE+ LG D P+VA+ NNLA + Q+KY+++E +Y+ dkfzphtes3 349 RALEIYATRLGPDDPNVAKTKNNLASCYLKQGKYQDAETLYK 390

DKFZphtes3_4ol9

group: testes derived

DKFZphtes3_4ol9 encodes a novel 1180 amino acid protein with weak similarity to human megakaryocyte stimulating factor and human mucin.

The novel protein contains a cytochrome c family heme-bindmg site signature. No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application m studying the expression profile of testis-specific genes . similarity to megakaryocyte stimulating factor and mucin complete cDNA, complete eds, EST hits (few)

Sequenced by AGOWA

Locus: unknown

Insert length: 3767 bp

Poly A stretch at pos. 3757, polyadenylation signal at pos. 3737

1 GGCTAGGTTT AGCTTCAGGG GCAGCCCAGG GCAGTGTTGC TGCATATTGC

51 ATGGATGAAA GGCTGAAGGC TGCCTCCTCT TGCAGGCTGG CTTCTGAGAT

101 TGCACCTTCT TCTCCTGCTA CTCCTCCAAA TCTATGACCC TTCAAGGCAG

151 AGCTGACCTG TCCGGTAATC AAGGCAATGC AGCCGGCCGC CTAGCTACAG

201 TTCACGAGCC AGTTGTCACC CAGTGGGCGG TGCATCCTCC AGCCCCCGCT

251 CACCCCAGTC TCCTGGACAA AATGGAGAAA GCGCCTCCAC AGCCCCAGCA

301 CGAGGGCCTC AAGTCCAAGG AGCATCTTCC GCAACAGCCT GCCGAAGGCA

351 AGACGGCGTC CCGCCGCGTC CCACGCCTCC GGGCTGTGGT CGAGAGCCAG

401 GCCTTCAAGA ACATCCTGGT AGACGAGATG GACATGATGC ACGCCCGTGC

451 AGCCACGCTC ATCCAAGCCA ACTGGAGGGG CTATTGGCTC CGGCAGAAGC

501 TGATTTCCCA GATGATGGCG GCCAAGGCCA TCCAGGAGGC CTGGCGGCGC

551 TTCAACAAGA GACACATCCT TCACTCCAGC AAGTCGTTGG TAAAGAAAAC

601 GAGGGCGGAG GAGGGGGACA TACCTTATCA CGCCCCACAG CAGGTGCGCT

651 TCCAGCATCC GGAAGAGAAC CGCCTTCTGT CCCCGCCCAT CATGGTGAAC

701 AAGGAGACCC AGTTCCCTTC CTGTGACAAT CTGGTCCTCT GCAGACCCCA

751 GTCGTCCCCC CTCCTGCAGC CCCCAGCAGC TCAGGGTACC CCAGAGCCCT

801 GTGTGCAGGG TCCTCATGCT GCCAGAGTCC GGGGGCTGGC CTTCCTGCCA

851 CACCAGACGG TCACCATCAG ATTTCCCTGC CCAGTGAGTT TGGACGCAAA

901 ATGCCAGCCA TGCCTGCTGA CCAGAACCAT CAGAAGCACC TGCCTCGTCC

951 ACATAGAGGG TGACTCAGTG AAGACCAAAC GTGTAAGTGC CCGGACCAAC

1001 AAAGCCAGGG CTCCGGAGAC ACCATTGTCC AGAAGGTATG ACCAGGCAGT

1051 TACGAGACCA TCCAGAGCCC AAACCCAGGG CCCTGTGAAA GCAGAGACCC

1101 CCAAAGCCCC CTTCCAGATA TGTCCAGGGC CCATGATCAC CAAGACTCTA

1151 CTCCAGACAT ATCCAGTGGT CTCCGTGACC CTGCCACAGA CATATCCAGC

1201 GTCCACGATG ACCACCACCC CACCCAAGAC TAGCCCAGTT CCCAAAGTAA

1251 CAATAATCAA GACCCCAGCC CAGATGTATC CGGGGCCCAC AGTGACCAAA

1301 ACTGCACCTC ACACATGCCC CATGCCCACA ATGACCAAGA TCCAGGTACA

1351 CCCCACAGCC TCCAGAACTG GCACCCCACG GCAGACATGC CCTGCGACCA

1401 TCACGGCAAA GAACCGACCT CAGGTTTCCC TTCTGGCTTC CATCATGAAG

1451 AGCCTGCCCC AGGTATGCCC GGGGCCTGCG ATGGCAAAGA CCCCACCCCA

1501 GATGCACCCG GTCACCACCC CAGCCAAAAA CCCATTGCAA ACATGTCTGT

1551 CAGCCACAAT GTCCAAGACT TCATCCCAGA GGAGCCCAGT TGGGGTGACC

1601 AAGCCCTCAC CCCAGACCCG CCTGCCAGCC ATGATAACCA AGACCCCAGC

1651 CCAGTTACGC TCGGTGGCCA CCATCCTCAA GACTCTGTGT CTGGCCTCTC

1701 CAACAGTGGC AAATGTCAAG GCTCCACCCC AAGTGGCGGT AGCAGCCGGA

1751 ACTCCCAACA CCTCAGGCTC CATCCATGAG AACCCACCCA AGGCCAAGGC

1801 CACCGTGAAT GTGAAGCAGG CTGCAAAGGT GGTGAAAGCC TCATCCCCCT

1851 CCTATTTGGC TGAGGGGAAG ATCAGGTGCC TGGCTCAACC ACATCCGGGA

1901 ACTGGGGTCC CCAGGGCTGC AGCTGAGCTT CCTTTGGAAG CCGAGAAAAT

1951 CAAGACTGGC ACCCAGAAAC AGGCGAAAAC AGACATGGCA TTTAAGACCA

2001 GTGTGGCAGT GGAAATGGCT GGGGCTCCAT CCTGGACAAA AGTTGCTGAG

2051 GAAGGGGACA AGCCACCTCA CGTGTATGTG CCTGTAGACA TGGCTGTCAC

2101 CCTGCCCCGG GGACAGCTGG CTGCCCCACT GACCAATGCC TCATCCCAGA

2151 GACATCCACC CTGCCTGTCC CAGAGACCAC TGGCCGCCCC GCTGACCAAG

2201 GCCTCATCTC AGGGACATCT GCCCACTGAG CTGACCAAGA CCCCATCCCT

2251 GGCCCATCTG GACACCTGTC TGAGCAAGAT GCATTCCCAG ACACATCTGG

2301 CCACAGGTGC CGTGAAGGTC CAGTCCCAAG CGCCTCTAGC CACCTGTCTG

2351 ACCAAGACGC AGTCCCGGGG GCAGCCGATC ACAGACATAA CCACGTGCCT

2401 CATCCCAGCG CACCAGGCTG CTGATCTCAG CAGCAACACC CACTCCCAGG

2451 TGCTCCTAAC AGGGTCCAAG GTGTCCAACC ACGCCTGCCA GCGCCTCGGT

2501 GGCCTCAGCG CCCCACCCTG GGCCAAGCCA GAGGACAGAC AGACCCAGCC

2551 ACAGCCCCAC GGACACGTGC CGGGGAAGAC CACTCAGGGG GGACCATGCC

2601 CGGCAGCCTG TGAGGTCCAG GGTATGCTGG TGCCGCCGAT GGCACCCACC 2651 GGCCATTCCA CATGCAACGT TGAGTCCTGG GGAGACAACG GAGCCACACG 2701 TGCCCAGCCA TCAATGCCCG GCCAGGCGGT GCCCTGCCAG GAGGACACGG 2751 GCCCCGCGGA CGCTGGTGTG GTTGGTGGCC AATCGTGGAA CCGCGCATGG 2801 GAGCCAGCCA GGGGTGCTGC GTCCTGGGAC ACCTGGCGCA ACAAGGCGGT 2851 GGTGCCTCCC AGGCGGTCCG GGGAGCCAAT GGTGTCCATG CAGGCTGCAG 2901 AGGAGATCCG CATCCTCGCA GTGATCACTA TCCAGGCGGG CGTCCGTGGC 2951 TACCTGGCGC GTCGCAGGAT CCGGCTGTGG CACCGGGGGG CCATGGTCAT 3001 CCAAGCTACT TGGCGCGGCT ACCGTGTGCG GCGGAACCTG GCACACCTCT 3051 GCAGAGCCAC CACGACCATC CAGTCTGCCT GGCGCGGCTA CAGCACCCGC 3101 CGGGACCAAG CCCGGCACTG GCAGATGCTC CACCCCGTCA CGTGGGTGGA 3151 GCTGGGCAGC CGGGCCGGGG TCATGTCTGA CCGAAGCTGG TTCCAGGATG 3201 GCAGAGCCAG GACAGTATCT GACCATCGCT GCTTCCAGTC CTGCCAGGCA 3251 CACGCTTGCA GCGTCTGCCA CTCCCTGAGC TCCAGGATCG GGAGCCCGCC 3301 CAGCGTGGTG ATGCTAGTGG GCTCCAGCCC TCGCACCTGT CATACCTGTG 3351 GACGCACACA GCCCACCCGT GTGGTGCAGG GCATGGGCCA GGGCACTGAG 3401 GGCCCCGGGG CAGTGTCTTG GGCCTCCGCC TACCAGCTGG CTGCCCTGAG 3451 TCCCAGGCAG CCGCATCGCC AGGACAAAGC GGCCACAGCC ATCCAGTCCG 3501 CCTGGAGGGG CTTTAAGATC CGCCAGCAGA TGAGGCAGCA GCAAATGGCA 3551 GCGAAGATAG TTCAAGCCAC CTGGCGAGGC CACCATACCC GGAGCTGTCT 3601 GAAGAACACA GAGGCGCTCT TGGGACCAGC AGACCCCTCG GCCAGCTCAC 3651 GGCACATGCA TTGGCCTGGC ATCTAGGACC CTGGCTCCCT GCAGTGGGGA 3701 CTTCGTGGGA GGCACTCATG GCTCTCTGGG TCTAATGAAT AAAGTCCTCC 3751 ACAGCCTAAA AAAAAAA

BLAST Results

No BLAST result

Medline entries

No Medl e entry

Peptide information for frame 2

ORF from 134 bp to 3673 bp; peptide length: 1180 Category: similarity to known protein

1 MTLQGRADLS GNQGNAAGRL ATVHEPVVTQ WAVHPPAPAH PSLLDKMEKA

51 PPQPQHEGLK SKEHLPQQPA EGKTASRRVP RLRAVVESQA FKNILVDEMD

101 MMHARAATLI QANWRGYWLR QKLISQMMAA KAIQEAWRRF NKRHILHSΞK

151 SLVKKTRAEE GDIPYHAPQQ VRFQHPEENR LLSPPIMVNK ETQFPSCDNL

201 VLCRPQSSPL LQPPAAQGTP EPCVQGPHAA RVRGLAFLPH QTVTIRFPCP

251 VSLDAKCQPC LLTRTIRSTC LVHIEGDSVK TKRVSARTNK ARAPETPLSR

301 RYDQAVTRPS RAQTQGPVKA ETPKAPFQIC PGPMITKTLL QTYPVVSVTL

351 PQTYPASTMT TTPPKTSPVP KVTIIKTPAQ MYPGPTVTKT APHTCPMPTM

401 TKIQVHPTAS RTGTPRQTCP ATITAKNRPQ VSLLASIMKS LPQVCPGPAM

451 AKTPPQMHPV TTPAKNPLQT CLSATMSKTS SQRSPVGVTK PSPQTRLPAM

501 ITKTPAQLRS VATILKTLCL ASPTVANVKA PPQVAVAAGT PNTSGSIHEN

551 PPKAKATVNV KQAAKVVKAS SPSYLAEGKI RCLAQPHPGT GVPRAAAELP

601 LEAEKIKTGT QKQAKTDMAF KTSVAVEMAG APSWTKVAEE GDKPPHVYVP

651 VDMAVTLPRG QLAAPLTNAS SQRHPPCLSQ RPLAAPLTKA SSQGHLPTEL

701 TKTPSLAHLD TCLSKMHSQT HLATGAVKVQ SQAPLATCLT KTQSRGQPIT

751 DITTCLIPAH QAADLSSNTH SQVLLTGSKV SNHACQRLGG LSAPPWAKPE

801 DRQTQPQPHG HVPGKTTQGG PCPAACEVQG MLVPPMAPTG HSTCNVESWG

851 DNGATRAQPS MPGQAVPCQE DTGPADAGVV GGQSWNRAWE PARGAASWDT

901 WRNKAVVPPR RSGEPMVSMQ AAEEIRILAV ITIQAGVRGY LARRRIRLWH

951 RGAMVIQATW RGYRVRRNLA HLCRATTTIQ SAWRGYSTRR DQARHWQMLH

1001 PVTWVELGSR AGVMSDRSWF QDGRARTVΞD HRCFQSCQAH ACSVCHSLΞS

1051 RIGSPPΞVVM LVGSSPRTCH TCGRTQPTRV VQGMGQGTEG PGAVSWASAY

1101 QLAALSPRQP HRQDKAATAI QSAWRGFKIR QQMRQQQMAA KIVQATWRGH

1151 HTRSCLKNTE ALLGPADPSA SSRHMHWPGI

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_4ol9, frame 2

TREMBL:HSU70136_1 product: "megakaryocyte stimulating factor"; Human megakaryocyte stimulating factor mRNA, complete eds., N = 2, Score = 242, P = 9.6e-16 TREMBL : HSMUC2A_1 gene: "MUC2"; product: "mucin"; Human mucιn-2 gene, partial eds., N = 1, Score = 204, P = 1.4e-12

PIR:S48478 glucan 1, 4-alpha-glucosιdase (EC 3.2.1.3) - yeast (Saccharomyces cerevisiae), N = 1, Score = 192, P = 9.6e-ll

>TREMBL:HSU70136_1 product: "megakaryocyte stimulating factor"; Human megakaryocyte stimulating factor mRNA, complete eds. Length = 1, 404

HSPs:

Score = 242 (36.3 bits), Expect = 9.6e-16, Sum P(2) = 9.6e-16 Identities = 145/546 (26%), Positives = 198/546 (36%)

Query: 282 KRVSARTNKARAPETPLSRRYDQAVTRPSRAQTQGPVKAETPKAPFQIC-PGPMITKTLL 340

K+ + T K AP TP PS + P T AP P P TK+

Sbjct: 488 KKPAPTTPKEPAPTTP-KEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAP 546

Query: 341 QTYPVVSVTLPQ TYPASTMTTTPPKTSPV-PKVTIIKTPAQMYPGPTVTKTAPHTC 395

T S T + T P TTP K +P PK TP + P PT TK Sbjct: 547 TTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKE—PAPTTTKK 599

Query: 396 PMPTMTKIQVHPTASRTGTPRQTCPATITAKNRPQVSLLASIMKSLPQVCPGPAMAKTPP 455

P PT K + PT TP++T P T LA P +A T P

Sbjct: 600 PAPTAPK-EPAPT TPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTP 653

Query: 456 QMHPVTTPAKNPLQTCLSATMSKTSSQRSPVGVTKPSPQT-RLPAMIT-KTPAQLRSVAT 513

+ TTP + P T A T + +P +P+P T + PA T K A T Sbjct: 654 EEPTPTTP-EEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGT 712

Query: 514 ILKTLCLASPTVANVKAPPQVAVAAG TPNTSGSIHENPPKAKATVNVKQAAKVV-KA 569

TL +PT AP ++A T TS PK A K+ A K Sbjct: 713 APTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTΞDKPAPTTPKGTAPTTPKEPAPTTPKE 772

Query: 570 SSPSYLAEGKIRCLAQPHPGTGVPRAAAELPLEAEKIKTGT—QKQAKTDMAFKTSVAVE 627

+P+ L +P P T A EL K T T K A T +T+

Sbjct: 773 PAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPK-ETAPTTP 831

Query: 628 MAGAPSWTKVAEEGDKPPHVYVPVDMAVTLPRGQLAAPLTNASSQRHPPCLSQRPLAAPL 687

AP+ K + P P V+ P + S P LS P L

Sbjct: 832 KEPAPTTPK—KPAPTTPETPPPTTΞEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKAL 889

Query: 688 TKASSQGHLPTELTKTPSLA--HLDTCLSKMHSQTHLATGAVKVQSQAPLAT—CLTKTQ 743

+ + +PT TKTP+ + T ++ L T + + AP T T T+ Sbjct: 890 ENSPKEPGVPT—TKTPAATKPEMTTTAKDKTTERDLRT-TPETTTAAPKMTKETATTTE 946

Query: 744 SRGQPITDITTCLIPAHQAADLS—SNTHSQVLLTGSKVSN—HACQRLGGLSAPP-WAK 798

+ TT + + D + T + KV+ ++ P AK

Sbjct: 947 KTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAK 1006

Query: 799 PEDRQTQPQPHGHVPGKTTQGGPCPAA 825

P+DR T + P K T+ P + Sbjct: 1007 PKDRATNSKATTPKPQKPTKAPKKPTS 1033

Score = 205 (30.8 bits), Expect = 3. le-12. Sum P(2) = 3. le-12 Identities = 146/565 (25%), Positives = 209/565 (36%)

Query: 281 TKRVSARTNKARAPETPLSRRYDQAVTRPSRAQTQGPVKAE—TPKAPFQICPGPMITKT 338

TK+ + K AP TP + A T P + P K TP+ P P + T Sbjct: 597 TKKPAPTAPKEPAPTTPK ETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTT 652

Query: 339 LLQTYPVVSVTLPQTYPASTMTTTPPKTSPV-PKVTIIKTPAQMYPGPTVTK-TAPHTCP 396

+ P T P + TP + +P PK TP + P PT K TAP T P Sbjct: 653 PEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKE—PAPTTPKETAP-TTP 709

Query: 397 M PTMTKIQVHPTASRTGTPRQTCPATITAKNRPQVSLLASIMKSLPQVCPGPAMAKT 453

PT K + PT + P++ P T + S + K P G A T Sbjct: 710 KGTAPTTLK-EPAPTTPKKPAPKELAPTT TKEPTSTTSD--KPAPTTPKGTAPT-T 761

Query: 454 PPQMHPVTTPAKNPLQTCLSATMSKTSSQRSPVGVTKPSPQTRLPAMITKTPAQLRSVAT 513

P + P TTP K P T T T + +P KP+P+ P TK P S Sbjct: 762 PKEPAP-TTP-KEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTT-TKGPTSTTSDKP 818

Query: 514 ILKTLCLASPTVANVKAPPQVAVAAGTPNTSGSIHENPPKAKATVNV KQAAKVVKA 569

T +PT AP A P T E PP + V+ K+ + K+ Sbjct: 819 APTTPKETAPTTPKEPAPTTPKKPA—PTTP ETPPPTTSEVSTPTTTKEPTTIHKS 872

Query: 570 SSPSYLAEGKIRCLAQPHPGTGVPRAAAELPLEAEKIKTGTQKQAKTDMAFKTSVAV 626

S+P AE + L GVP + P + T T K T+ +T+ Sbjct: 873 PDESTPELSAEPTPKALENSPKEPGVP—TTKTPAATKPEMTTTAKDKTTERDLRTTPET 930

Query: 627 EMAGAPSWTK-VAEEGDKPPHVYVPVDMAVTLPRGQLAAPLTNASSQRHPPCLSQRPLAA 685

A AP TK A +K + +T Q+ + T ++ L LA Sbjct: 931 TTA-APKMTKETATTTEKT TESKITATTTQVTSTTTQDTTPFKITTLKTTTLAP 983

Query: 686 PLTKASSQGHLPTELTKTPSLAHLDTCLSKMHSQTHLATGAVKVQS QAPLATCLT 740

+T + + TE+ P +T K + AT K Q + P +T Sbjct: 984 KVT-TTKKTITTTEIMNKPE ETAKPKDRATNSKAT-TPKPQKPTKAPKKPTSTKKP 1037

Query: 741 KTQSR-GQPITDIT TCLIPAHQAADLSΞNTHSQVLLTGSKVSNHACQRLGGLSAPP 795

KT R +P T T T +P + Q ++ N + S Sbjct: 1038 KTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDA 1097

Query: 796 W-AKPEDRQTQPQPHGHVPGKTTQGGPCPAACEVQGMLVPPMAPTGHSTCN 845

A+ E +PH +P T P QG+++ PM + CN Sbjct: 1098 GGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPN-QGIIINPMLΞDETNICN 1147

Score = 198 (29.7 bits), Expect = 2.3e-ll, Sum P(2) = 2.3e-ll Identities = 142/513 (27%), Positives = 200/513 (38%)

Query: 204 RPQSSPLLQPPAAQGTPEPCVQGPHAARVRGLAFLPHQTVTIRFPCPVSLDAKCQPCLLT 263

R + P +PP G + H V+ + +P L

Sbjct: 207 RTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPΞLPP 266

Query: 264 R—TIRSTCLVHIEGDΞVKTKRVSARTNKARAP ETPLSRRYDQAVTRPSR AQTQ 315

T + T L + +V+TK + TNK + E S + Q++ + S A T Sbjct: 267 NSDTSKETSLTVNKETTVETKETTT-TNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTS 325

Query: 316 GPVKAETPKAPFQICPGPMITKTLLQTYPVVSVTLPQTYPASTMTTTPPKTSPVPKVTII 375

+ TPKA GP +T T + P T P+ PAST TP + +P + Sbjct: 326 KVLAKPTPKAE-TTTKGPALT-TPKEPTP TTPKE-PAST TPKEPTPTTIKSAP 375

Query: 376 KTPAQMYPGPTVTKTAPHTC—PMPTMTKIQVHPTASRTGTPRQTC-PATITAKNRPQVS 432

TP + P PT TK+AP T P PT TK + PT + P T PA T K+ P Sbjct: 376 TTPKE—PAPTTTKSAPTTPKEPAPTTTK-EPAPTTPKEPAPTTTKEPAPTTTKSAPTTP 432

Query: 433 LLASIMKSLPQVCPGPAMAKTPPQMHPVTTPAKNPLQTCLSATMSKTSSQRSPVGVT 489

+ K P PA TP + P TTP K P T + T + +P Sbjct: 433 KEPAPTTPKKPAPTTPKEPAPT-TPKEPTP-TTP-KEPAPTTKEPAPT-TPKEPAPTAPK 488

Query: 490 KPSPQT-RLPAMIT-KTPAQLRSVA TILK TLCLASPTVANVKAPPQVAVAAGT 540

KP+P T + PA T K PA + T K T ++PT AP A T Sbjct: 489 KPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTT 548

Query: 541 PNT-SGSIHENP PKAKATVNVKQAAKVV-KASSPSYLAEGKIRCLAQPHPGTGVPR 594

P S + + P PK A K+ A K +P+ E +P P P+ Sbjct: 549 PKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTA--PK 606

Query: 595 AAAELPLEAEKIKTGTQKQAKTDMAFKTSVAVEMAGAPSWTK-VAEEGDKPPHVYVPVDM 653

A^' P ++ T K+ K + AP+ + +A + P P + Sbjct: 607 EPA—PTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEP 664

Query: 654 AVTLPRGQLAAPLTNASSQRHP-PCLSQRPLAAPLTKASSQGHLPTELTKTPSLAHLDTC 712

A T P+ AAP T + P P + P AP T P E T T Sbjct: 665 APTTPKA—AAPNT PKEPAPTTPKEP—APTTPKEPAPTTPKETAPTTPKGTAPTT 716

Query: 713 LSK 715

L + Sbjct: 717 LKE 719

Score = 108 (16.2 bits). Expect = 4.3e-02, Sum P(2) = 4.3e-02 Identities = 60/214 (28%), Positives = 85/214 (39%)

Query: 265 TIRSTCLVHIEGDSVKTKRVSAR-TNKA—RAPETP-LSRRYDQAVTRPSRAQTQGPVKA 320

T + +H D T +SA T KA +P+ P + A T+P T Sbjct: 862 TTKEPTTIHKSPDE-STPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTT 920

Query: 321 ETP—KAPFQICPGPMITK-TLLQTYPVVSVTLPQTYPASTMTTTPPKTSPVPKVTIIKT 377

E P P +TK T T + T T TTT T+P K+T +KT Sbjct: 921 ERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQD-TTPF-KITTLKT 978

Query: 378 PAQMYPGPTVTK TAPHTCPMPTMT-KIQVHPTASRTGTPRQTCPATITAKNRPQVSL 433

+ P T TK T P T K + T S+ TP+ P A +P + Sbjct: 979 TT-LAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTK—APKKPTSTK 1035

Query: 434 LASIMKSL—PQVCPGPA-MAKTPPQMHPVTTPAKNPLQT 470

M + P+ P P M T P+++P + A+ LQT Sbjct: 1036 KPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQT 1075

Score = 56 (8.4 bits), Expect = 3. le-12, Sum P(2) = 3. le-12 Identities = 17/60 (28%), Positives = 22/60 (36%)

Query: 22 TVHEPVVTQWAVHPPAPAHPSLLDKMEKAPPQPQHEGLKS-KEHLPQQPAEGKTASRRVP 80

T EP T P P PS E AP P+ + K+ P P E + + P Sb_jct: 533 TTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEP 592

Score = 52 (7.8 bits), Expect = 9.6e-16, Sum P(2) = 9.6e-16 Identities = 17/59 (28%), Positives = 22/59 (37%)

Query: 22 TVHEPV-VTQWAVHPPAPAHPSLLDKMEKAPPQPQHEGLKSKEHLPQQPAE-GKTAΞRR 78

T EP T P P P+ E P P+ +KE P P E TA ++ Sbjct: 431 TPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKK 489

Score = 51 (7.7 bits), Expect = 1.2e-15, Sum P(2) = 1.2e-15 Identities = 15/51 (29%), Positives = 19/51 (37%)

Query: 22 TVHEPVVTQWAVHPPAPAHPSLLDKMEKAPPQPQHEGLKS-KEHLPQQPAE 71

T EP T P P P+ + AP P+ + KE P P E Sbjct: 416 TTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKE 466

Score = 47 (7.1 bits), Expect = 3.2e-15, Sum P(2) = 3.2e-15 Identities = 12/41 (29%), Positives = 17/41 (41%)

Query: 36 PAPAHPSLLDKMEKAPPQPQHEGLKSKEHLPQQPAEGKTAS 76

P P P + P +P +KS P++PA T S Sbjct: 350 PTPTTPK—EPASTTPKEPTPTTIKSAPTTPKEPAPTTTKS 388

Score = 47 (7.1 bits), Expect = 3.2e-15, Sum P(2) = 3.2e-15 Identities = 15/57 (26%), Positives = 19/57 (33%)

Query: 22 TVHEPVVTQWAVHPPAPAHPSLLDKMEKAPPQPQHEG-LKSKEHLPQQPAEGKTASR 77

T EP T P P P+ E AP P+ +KE P T + Sbjct: 377 TPKEPAPTTTKΞAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPK 433

Score = 46 (6.9 bits), Expect = 4.0e-15, Sum P(2) = 4.0e-15 Identities = 16/58 (27%), Positives = 22/58 (37%)

Query: 20 LATVHEPVVT QWAVHPPAPAHPSLLDKMEKAPPQPQHEGLKSKEHLPQQPAEGKT 74

L T EP T + A P P+ + P +P KS P++PA T Sbjct: 344 LTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTT 401

Score = 42 (6.3 bits), Expect = 1.0e-14, Sum P(2) = 1.0e-14 Identities = 15/60 (25%), Positives = 21/60 (35%)

Query: 22 TVHEPVVTQWAVHPPAPAHPSLLDKMEKAPPQPQHEGLKS-KEHLPQQPAEGKTAΞRRVP 80

T EP T P P P+ + AP P+ + KE P E + + P Sbjct: 463 TPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEP 522

Score = 39 (5.9 bits), Expect = 2. le-14, Sum P(2) = 2. le-14 Identities = 15/55 (27%), Positives = 20/55 (36%)

Query: 22 TVHEPVVTQWAVHPPAPAHPΞLLDKMEKAPPQPQHEGLKSKEHLPQQPAEGKTAΞ 76

T EP T P PA + + P +P KS ++PA T S Sbjct: 494 TPKEPAPTT PKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKS 544

Pedant information for DKFZphtes3_4ol9, frame 2

Report for DKFZphtes3_4ol9.2

[LENGTH] 1180

[MW] 127693.40

[pi] 10.25

[HOMOL] SWISSPROT :MUC2_HUMAN MUCIN 2 PRECURSOR (INTESTINAL MUCIN 2). le-08

[FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YJR151C] 6e-06

[FUNCAT] 30.01 organization of cell wall [S. cerevisiae, YIR019c] 6e-06

[FUNCAT] 30.90 extracellular/secretion proteins [S. cerevisiae, YIR019c] 6e-06

[FUNCAT] 01.05.01 carbohydrate utilization [S. cerevisiae, YIR019c] 6e-06

[BLOCKS] BL00412B Neuromodulin (GAP-43) proteins

[PROSITE] CYTOCHROME_C 1

[PROSITE] MYRISTYL 12

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 8

[PROSITE] PKC_PHOSPHO_SITE 25

[PROSITE] ASNJΞLYCOSYLATION 2

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 5.00 % SEQ MTLQGRADLSGNQGNAAGRLATVHEPWTQWAVHPPAPAHPSLLDKMEKAPPQPQHEGLK

SEG

PRD cccccceeeccccccceeeeeeeeceeeeeeeecccccccceeeeccccccccccccccc

SEQ ΞKEHLPQQPAEGKTASRRVPRLRAVVESQAFKNILVDEMDMMHARAATLIQANWRGYWLR

SEG

PRD cccccccccccccccccchhhhhhhhhhhhhhheeehhhhhhhhhhhhhhhhhccchhhh

SEQ QKLISQMMAAKAIQEAWRRFNKRHILHSSKSLVKKTRAEEGDIPYHAPQQVRFQHPEENR

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhheeeecccchhhhhhhhcccccccccceeeecccccce

SEQ LLSPPIMVNKETQFPSCDNLVLCRPQSSPLLQPPAAQGTPEPCVQGPHAARVRGLAFLPH

SEG

PRD eeccceeeecccccccccceeeecccccccccccccccccccccccccceeeeeeeeccc

SEQ QTVTIRFPCPVSLDAKCQPCLLTRTIRSTCLVHIEGDSVKTKRVSARTNKARAPETPLSR

SEG

PRD eeeeeecccccccccccccccccccccceeeeecccccccceeeeecccccccccccccc

SEQ RYDQAVTRPSRAQTQGPVKAETPKAPFQICPGPMITKTLLQTYPVVSVTLPQTYPASTMT

SEG xxxx

PRD ccceeeeeccccccccceeecccccccccccccccccccccccccccccccccccccccc

SEQ TTPPKTSPVPKVTIIKTPAQMYPGPTVTKTAPHTCPMPTMTKIQVHPTAΞRTGTPRQTCP

SEG xxxxxxxxxxxxx

PRD cccccccccccceeeccccccccccccccccccccccccccceeeccccccccccccccc

SEQ ATITAKNRPQVSLLASIMKSLPQVCPGPAMAKTPPQMHPVTTPAKNPLQTCLSATMSKTS

SEG

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ SQRSPVGVTKPSPQTRLPAMITKTPAQLRSVATILKTLCLASPTVANVKAPPQVAVAAGT

SEG

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ PNTSGSIHENPPKAKATVNVKQAAKVVKASSPSYLAEGKIRCLAQPHPGTGVPRAAAELP

SEG xxxxxxxxxxxxxxxxx xxxxxx

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ LEAEKIKTGTQKQAKTDMAFKTSVAVEMAGAPSWTKVAEEGDKPPHVYVPVDMAVTLPRG

SEG xxxx

PRD ccccccccccccccccccccccccccccccccccceeeeccccccceeeccccccccccc

SEQ QLAAPLTNASSQRHPPCLSQRPLAAPLTKASSQGHLPTELTKTPSLAHLDTCLSKMHSQT

SEG

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ HLATGAVKVQSQAPLATCLTKTQSRGQPITDITTCLIPAHQAADLSSNTHSQVLLTGSKV

SEG

PRD ccccceeeeeccccceeeeccccccccccccccccccccccccccccccceeeeeccccc

SEQ SNHACQRLGGLSAPPWAKPEDRQTQPQPHGHVPGKTTQGGPCPAACEVQGMLVPPMAPTG

SEG

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ HSTCNVESWGDNGATRAQPSMPGQAVPCQEDTGPADAGVVGGQSWNRAWEPARGAASWDT

SEG

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ WRNKAVVPPRRSGEPMVSMQAAEEIRILAVITIQAGVRGYLARRRIRLWHRGAMVIQATW

SEG

PRD ccceeecccccccccchhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhh

SEQ RGYRVRRNLAHLCRATTTIQSAWRGYSTRRDQARHWQMLHPVTWVELGSRAGVMSDRSWF

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhheeeeeccchhhhhhhhhhh

SEQ QDGRARTVSDHRCFQSCQAHACSVCHSLSSRIGSPPSVVMLVGSSPRTCHTCGRTQPTRV

SEG

PRD hccceeeeccceeeecccceeeeeeeecccccccccceeeeeecccccccccccccccee

SEQ VQGMGQGTEGPGAVSWASAYQLAALSPRQPHRQDKAATAIQΞAWRGFKIRQQMRQQQMAA

SEG xxxxxxxxxxxxx

PRD eeeccccccccccchhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ KIVQATWRGHHTRSCLKNTEALLGPADPSASSRHMHWPGI

SEG XX

PRD hhhhhhhccccccchhhhhhhhcccccccccccccccccc cocn w en en w ω en en ω co co co ω ω c cn ω w en en w w w co en w cn cn w co en ω en w

0000000000000000000000000000000000000000000000000

0000000000000000000000000000000000000000000000000 t-'OO OOOO OOOO OOOOOOOOOOO OOOOOOOOOOOOOOO OOOOO OOO O O OO VDO O OOO O OOO O OOO O OOOOOO O OOOO O OOO OO OOO O O OOOO O OOO O OOO

DKFZphtes3_50j4

group: testes derived

DKFZphtes3_50j4 encodes a novel 187 ammo acid protein proline rich protein.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application m studying the expression profile of testis-specific genes . unknown, prolin ritch protein complete cDNA, complete eds, EST hits

Sequenced by DKFZ

Locus : unknown

Insert length: 1186 bp

Poly A stretch at pos. 1176, polyadenylation signal at pos. 1126

1 CACTGGGCGT CTGAAGCTCA GAGCTCACCC CTGAGATGGG CTCTCCTAGG

51 CCTCCTGGGA TGAGGGAGCC ACCAGGACCC AGTGCTGTGA TGCCTGCTCT

101 TCCCTCTACC AGCACCTGCC CGCCCAGAGA CCAGGGCACC CCTGAAGTCC

151 AGCCCACCCC TGCAAAGGAC ACATGGAAGG GCAAGCGGCC TCGATCCCAG

201 CAGGAGAACC CAGAGAGCCA GCCTCAGAAG AGGCCACGCC CCTCAGCCAA

251 GCCCTCCGTC GTAGCTGAGG TCAAGGGCAG CGTCTCGGCC AGCGAACAGG

301 GCACCTTGAA TCCCACGGCT CAAGACCCCT TCCAGCTCTC CGCTCCTGGC

351 GTCTCCTTGA AGGAGGCTGC AAATGTTGTG GTCAAGTGCC TCACCCCTTT

401 CTACAAGGAG GGCAAGTTTG CTTCCAAGGA GTTGTTTAAA GGCTTTGCCC

451 GCCACCTCTC ACACTTGCTG ACTCAGAAGA CCTCTCCTGG AAGGAGCGTG

501 AAAGAAGAGG CCCAGAACCT CATCAGGCAC TTCTTCCATG GCCGGGCCCG

551 GTGCGAGAGC GAAGCTGACT GGCATGGCCT GTGTGGCCCC CAGAGATGAC

601 CAACTGCTGG CTGGGCAGGG CCCGCGTCCT CCCCCAGATT CTAGCATGGG

651 TCATCCTGGG CCTCACCTGC TGATGCCAGG GCCATCGTCT TTTCTCAGTC

701 CTTCTCCTTT CCAACCATAC TTGGCTTTGG GGATGACCCC AGACACCCCC

751 TGAATCCAGG TCAGAGGTCA GCCCACCTTT CTTTCTGCTT GCAAAGCCTA

801 TAGACCCTTC TCAGAGCGGT CCTCATGGCT GGGTTTTCTG GGACACATGT

851 CGAGGACAGA AGGTGGAGGG TGGTGGAGCT GCTGCTGGAA GAAGGGGAAG

901 GAAGAGTGGC CCCTCCCCGA GTTCTAAGTC AGGATGAGGC CCACCTGTCC

951 AAGGTATCGG AACCTACCCA GGGGACCCTC AGATCCTCCA CCCACTCCCC

1001 CATCCATTAC GATGCCAGCT TCCAGCCTTG CCCAGGTCAG AGCTGTGGCA

1051 GAGGAGAGGC AGCCAGGCCC TGTTCCTGCT CAGCTCCTGC TCAGGAAGGC

1101 CAGGCCTGAC AGATGTTTGG GAGAGGAATA AAGTTGTGTT GTTGTGGGGC

1151 ATGCAGGCGT GCACACAGCC CTTTTCAAAA AAAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 36 bp to 596 bp; peptide length: 187 Category: putative protein

1 MGSPRPPGMR EPPGPSAVMP ALPSTSTCPP RDQGTPEVQP TPAKDTWKGK

51 RPRSQQENPE SQPQKRPRPS AKPSVVAEVK GSVSASEQGT LNPTAQDPFQ

101 LSAPGVSLKE AANVVVKCLT PFYKEGKFAS KELFKGFARH LSHLLTQKTS

151 PGRSVKEEAQ NLIRHFFHGR ARCESEADWH GLCGPQR

BLASTP hits

Entry MMU92455_1 from database TREMBL: product: "WW domain binding protein 7"; Mus musculus WW domain binding protein 7 mRNA, partial eds.

Score = 134, P = 6.9e-08, identities = 45/125, positives = 56/125

Alert BLASTP hits for DKFZphtes3_50j 4, frame 3 No Alert BLASTP hits found

Pedant information for DKFZphtes3_50j4, frame 3

Report for DKFZphtes3_50j4.3

[LENGTH] 187

[MW] 20353.06

[pi] 9.76

[PROSITE] MYRISTYL 1

[PROSITE] AMIDATION 1

[PROSITE] CK2_PHOSPHO_SITE

[PROSITE] PKC_PHOSPHO_SITE

[KW] All_Alpha

[KW] LOW COMPLEXITY 1.56 %

SEQ MGSPRPPGMREPPGPSAVMPALPSTSTCPPRDQGTPEVQPTPAKDTWKGKRPRSQQENPE SEG xxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ SQPQKRPRPSAKPΞVVAEVKGSVSASEQGTLNPTAQDPFQLSAPGVSLKEAANVVVKCLT SEG PRD cccccccccccccchhhhhccccccccccccccccccccccccccccchhhhhhheeecc

SEQ PFYKEGKFASKELFKGFARHLSHLLTQKTSPGRSVKEEAQNLIRHFFHGRARCESEADWH SEG PRD cccccccchhhhhhhhhhhhhhhhheeecccccchhhhhhhhhhhhhhccchhhhhhhhh

SEQ GLCGPQR SEG PRD ccccccc

Prosite for DKFZphtes3_50j4.3

PS00005 3->6 PKC PHOSPHO SITE PDOC00005

PS00005 46->49 PKC PHOSPHO^{" "}SITE PDOC00005

PS00005 70->73 PKC PHOSPHO^{" "}SITE PDOC00005

PS00005 107-M10 PKC PHOSPHO^{" "}SITE PDOC00005

PS00005 146->149 PKC PHOSPHO^{" "}SITE PDOC00005

PS00005 154->157 PKC PHOSPHO^{" "}SITE PDOC00005

PS00006 54->58 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 84->88 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 94->98 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 107->111 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 154->158 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 175->179 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00008 81->87 MYRISTYL PDOC00008

PS00009 48->52 AMIDATION PDOC00009

(No Pfam data available for DKFZphtes3_50j 4.3) DKFZphtes3_50n06

group: testes derived

DKFZphtes3_50n06 encodes a novel 186 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application n studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, EST hits

Sequenced by DKFZ

Locus : unknown

Insert length: 1095 bp

Poly A stretch at pos. 1085, polyadenylation signal at pos. 1061

1 CAAGACCCTC GGAGCCAAGA AACAACACTG AGTTCCAGAT TTCGGAAGGT 51 TCACGAGTGT TGCCGACACG CCCTCCCAAC TGCAGACATC CTCCCTGGAG

101 GACCTGCTGT GCTCACATGC CCCCCTGTCC AGCGAGGACG ACACCTCCCC

151 GGGCTGTGCA GCCCCCTCCC AGGCACCCTT CAAGGCCTTC CTCAGTCCCC

201 CAGAGCCACA TAGCCACCGA GGCACCGACA GGAAGCTGTC CCCGCTCCTG

251 AGCCCCTTGC AAGACTCACT GGTGGACAAG ACCCTGCTGG AGCCCAGGGA

301 GATGGTCCGG CCTAAGAAGG TGTGTTTCTC GGAGAGCAGC CTGCCCACCG

351 GGGACAGGAC CAGGAGGAGC TACTACCTCA ATGAGATCCA GAGCTTCGCG

401 GGCGCCGAGA AGGACGCGCG CGTGGTGGGC GAGATCGCCT TCCAGCTGGA

451 CCGCCGCATC CTGGCCTACG TGTTCCCGGG CGTGACGCGG CTCTACGGCT

501 TCACGGTGGC CAACATCCCC GAGAAGATCG AGCAGACCTC CACCAAGTCT

551 CTGGACGGCT CCGTGGACGA GAGGAAGCTG CGCGAGCTGA CGCAGCGCTA

601 CCTGGCCCTG AGCGCGCGCC TGGAGAAGCT GGGCTACAGC CGCGACGTGC

651 ACCCGGCGTT CAGCGAGTTC CTCATCAACA CCTACGGAAT CCTGAAGCAG

701 CGGCCCGACC TGCGCGCCAA CCCCCTGCAC AGCAGCCCGG CCGCGCTGCG

751 CAAGCTGGTC ATCGACGTGG TGCCCCCCAA GTTCCTGGGC GACTCGCTGC

801 TGCTGCTCAA CTGCCTGTGC GAGCTCTCCA AGGAGGACGG CAAGCCCCTC

851 TTCGCCTGGT GAGCCGCCCC GCGCCCGCCG CCTTGCCTGC AGTAAACGCG

901 TTTGTTCCAA CCCGGGGCCG CGGTGCCTCC TGCGCGTCCC CCCGGAGGGG

951 AAAGGGCCGC GTCCCCCGCG CGCGAGGCCA GAGAAGGCCC CGCTCCCACC 1001 GGTGCTGGGC CCCGACCGCA GCCCGCCGCT GCCCGCACCT GCGGAGTGCT 1051 TCTCACCCCT CATTAAAATC ATCCGTTTGC TTGTCAAAAA AAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 302 bp to 859 bp; peptide length: 186 Category: putative protein Classification: no clue

1 MVRPKKVCFS ESΞLPTGDRT RRSYYLNEIQ SFAGAEKDAR VVGEIAFQLD

51 RRILAYVFPG VTRLYGFTVA NIPEKIEQTS TKSLDGSVDE RKLRELTQRY

101 LALSARLEKL GYSRDVHPAF SEFLINTYGI LKQRPDLRAN PLHSSPAALR

151 KLVIDVVPPK FLGDSLLLLN CLCELSKEDG KPLFAW

BLASTP hits

No BLASTP hits available Alert BLASTP hits for DKFZphtes3_50n06, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphtes3_50n06, frame 2

Report for DKFZphtes3_50n06.2

[LENGTH] 186

[MW] 21049.39

[pi] 9.28

[KW] All Alpha

[KW] LOW COMPLEXITY 5.38 %

SEQ MVRPKKVCFSESSLPTGDRTRRSYYLNEIQSFAGAEKDARVVGEIAFQLDRRILAYVFPG

SEG

PRD ccccceeeccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccc

SEQ VTRLYGFTVANIPEKIEQTSTKSLDGSVDERKLRELTQRYLALSARLEKLGYSRDVHPAF

SEG

PRD ceeeeeeeeeeccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhccccccccch

SEQ SEFLINTYGILKQRPDLRANPLHSSPAALRKLVIDVVPPKFLGDSLLLLNCLCELSKEDG

SEG xxxxxxxxxx

PRD hhhhhhcceeecccccccccccccchhhhhhhhhhccccccccchhhhhhhhhhhhcccc

SEQ KPLFAW

SEG

PRD cccccc

(No Prosite data available for DKFZphtes3_50n06.2) (No Pfam data available for DKFZphtes3 50n06.2)

DKFZphtes3_50n23

group: testes derived

DKFZphtes3_50n23 encodes a novel 499 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes .

unknown

(from other testis librarys) testis specific cDNA'

Sequenced by DKFZ

Locus: unknown

Insert length: 1907 bp

Poly A stretch at pos. 1897, polyadenylation signal at pos. 1872

1 GGGCACCAGC CACTTTCCAC CATGACTGTG CGCTCGAGGG TCGCAGATGT

51 GTTCGGCAGC AAGGACACTG AGAGCCTTGA GCCTGTGCTT TTACCCTTAG

101 TAGATCGCAG GTTTCCTAAG AAATGGGAAA GACCGGTGGC AGAAAGCTTA

151 GGCCACAAAG ACAAAGACCA GGAGGACTAC TTCCAGAAGG GAGGACTCCA

201 AATTAAGTTC CACTGTAGCA AGCAGCTGTC TCTAGAGAGC TCCAGGCAGG

251 TGACCTCTGA GAGCCAAGAG GAGCCCTGGG AGGAGGAATT CGGCCGGGAG

301 ATGCGGAGGC AGCTGTGGCT GGAGGAGGAG GAGATGTGGC AGCAGCGGCA

351 GAAGAAGTGG GCCCTGCTGG AGCAGGAGCA TCAGGAGAAG CTGCGGCAGT

401 GGAATCTGGA AGACCTGGCC AGGGAGCAAC AGCGGAGATG GGTCCAGCTA

451 GAAAAGGAGC AGGAGAGCCC ACGGAGAGAG CCAGAGCAGC TAGGGGAGGA

501 TGTGGAGAGG AGGATCTTCA CACCCACCAG TCGATGGAGG GACTTGGAGA

551 AGGCAGAGCT ATCATTAGTG CCTGCCCCAA GCCGGACCCA ATCTGCTCAC

601 CAAAGCAGGA GGCCACACTT GCCCATGTCT CCTAGTACCC AGCAGCCTGC

651 CCTGGGAAAG CAGAGACCTA TGAGTTCAGT GGAGTTTACC TACAGACCAC

701 GGACCCGCCG AGTTCCCACA AAGCCCAAGA AATCTGCCTC CTTTCCTGTC

751 ACTGGGACAT CCATCCGAAG GCTGACCTGG CCCTCTTTGC AGATATCCCC

801 TGCAAATATT AAGAAGAAGG TGTACCACAT GGACATGGAG GCCCAGAGGA

851 AGAACCTGCA GCTCCTGAGT GAGGAGTCTG AGTTGAGGCT GCCCCACTAC

901 CTGCGCAGCA AAGCACTGGA GCTCACCACC ACCACCATGG AGCTGGGCGC

951 GCTCAGGCTG CAGTACCTGT GCCATAAGTA CATCTTCTAT AGACGCCTCC

1001 AGAGCCTCCG GCAAGAAGCG ATCAACCATG TACAAATCAT GAAAGAAACG

1051 GAGGCTTCCT ACAAGGCCCA GAACCTCTAC ATCTTCCTGG AAAACATTGA

1101 CCGCCTGCAG AGTCTCAGGC TGCAGGCCTG GACGGACAAG CAGAAGGGGC

1151 TGGAGGAGAA GCACCGAGAG TGCCTGAGCA GCATGGTGAC CATGTTCCCC

1201 AAGCTCCAGC TGGAGTGGAA CGTTCACCTG AACATCCCTG AGGTCACCTC

1251 GCCAAAGCCA AAGAAATGCA AGTTGCCTGC AGCCTCACCC CGGCACATCC

1301 GCCCCAGTGG CCCCACCTAC AAGCAGCCCT TTCTGTCTAG GCACCGGGCA

1351 TGTGTGCCCC TGCAGATGGC CCGCCAACAG GGGAAGCAGA TGGAGGCTGT

1401 CTGGAAGACC GAGGTGGCCT CCTCCAGTTA CGCAATAGAA AAAAAGACCC

1451 CTGCCAGCCT TCCCCGGGAC CAGCTGAGGG GACACCCAGA TATTCCCCGG

1501 CTGTTGACAC TGGACGTGTA GTCCTCCTGC CACAAAAGCC TGAACTTCCT

1551 GAAGGCCCAG TAAGCGCCTC AGCGAACCAA AGGAAGGAAT GCCAGGAACC

1601 TACAAATGAA TCCGCTTAGC TTGTTCAAAA AAAGTCAAGC GAGTCACTCC

1651 CTGGAACCCA AATAAGCCAG AAGGATCAAG ACAGCCCCAG TCTCCACTGC

1701 ATCCCTCAGC CAGTGATTCT CAACCTTCTG AGGGACGGAA ACCCACAGAG

1751 AACTTGGTCA AAATGCAGGT TCCCAGCTGG TGCTTTTAAA GAAACCCTCT

1801 GGGGGTTGCT GAGTACTCCT AGAACTTTGA GAAACACTGC TTCCCTCCTG

1851 CAGTCCCCAA ACTCTACATT TTAATAAAAT AGAGGTTGGT TTATTTTAAA

1901 AAAAAAA

BLAST Results o BLAST result

Medlme entries o Medline entry Peptide information for frame 1

ORF from 22 bp to 1518 bp; peptide length: 499 Category: similarity to known protein Classification: no clue

1 MTVRSRVADV FGSKDTESLE PVLLPLVDRR FPKKWERPVA EΞLGHKDKDQ

51 EDYFQKGGLQ IKFHCSKQLS LESSRQVTSE SQEEPWEEEF GREMRRQLWL

101 EEEEMWQQRQ KKWALLEQEH QEKLRQWNLE DLAREQQRRW VQLEKEQESP

151 RREPEQLGED VERRIFTPTS RWRDLEKAEL SLVPAPSRTQ SAHQSRRPHL

201 PMSPSTQQPA LGKQRPMSSV EFTYRPRTRR VPTKPKKSAS FPVTGTSIRR

251 LTWPSLQISP ANIKKKVYHM DMEAQRKNLQ LLSEESELRL PHYLRSKALE

301 LTTTTMELGA LRLQYLCHKY IFYRRLQSLR QEAINHVQIM KETEASYKAQ

351 NLYIFLENID RLQSLRLQAW TDKQKGLEEK HRECLSSMVT MFPKLQLEWN

401 VHLNIPEVTS PKPKKCKLPA ASPRHIRPSG PTYKQPFLSR HRACVPLQMA

451 RQQGKQMEAV WKTEVASSSY AIEKKTPASL PRDQLRGHPD IPRLLTLDV

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_50n23, frame 1

PIR:S28589 trichohyalm - rabbit, N = 1, Score = 134, P = 5.3e-05

TREMBLNEW:AF132479_1 product: "Ese2L protein"; Mus musculus Ese2L protein mRNA, complete eds., N = 1, Score = 130, P = 0.00017

>PIR:S28589 trichohyalm - rabbit Length = 1,407

HSPs:

Score = 134 (20.1 bits), Expect = 5.3e-05, P = 5.3e-05 Identities = 88/354 (24%), Positives = 154/354 (43%)

Query: 29 RRFPKKWERPVAESLGHKDKDQEDYFQKGGLQIK-FHCSKQLSLESSRQVTSESQEEPWE 87

R++ K +R + L + ++E ++ G + F +QL +++ E +EE + Sbjct: 165 RQYRDKEQRLQRQELEERRAEEEQLRRRKGRDAEEFIEEEQLRRREQQELKRELREEEQQ 224

Query: 88 EEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNLEDLAREQQRRWVQLEKEQ 147

RE + L+EEE RQ++W E Q++LR+ LE++ RE+++R Q E+ + Sbjct: 225 RRERREQHERA-LQEEEEQLLRQRRWRE-EPREQQQLRR-ELEEI-REREQRLEQEERRE 280

Query: 148 ESPRREPEQLGEDVERRIFTPTSRWRDLEKAELSLVPAPSRTQSAHQSRRPHLPMSPSTQ 207

+ RRE ++L E ERR ++ + E L R Q Q R + +

Sbjct: 281 QQLRRE-QRL-EQEERREQQLRRELEEIREREQRLEQEERREQRLEQEERREQQLKRELE 338

Query: 208 QPALGKQRPMSSVEFTYRPRTRRVPTKPKKSASFPVTGTSIRRLTWPSLQISPANIKK-K 266

+ +QR +E R R + + + ++ A G S+ R W S A ++ K Sbjct: 339 EIREREQR LEQEER-REQLLAEEVREQAR—ERGESLTR-RWQRQLESEAGARQΞK 390

Query: 267 VYHMDMEAQRKNLQLLSEESELRLPHYLRSKALELTTTTM ELGALRLQYLCHKY 320

VY +R+ Q L ++ E R R + LE E R Q L +

Sbjct: 391 VYS RPRRQEEQSLRQDQERR-QRQERERELEEQARRQQQWQAEEESERRRQRLSARP 446

Query: 321 IFYRRLQSLRQEAINHVQIMKETEASYKAQNLYI-FLENIDRLQΞL-RLQAWTDKQKGLE 378

R Q +E Q +E E + + + FLE ++LQ R Q ++ E Sbjct: 447 SLRER-QLRAEERQEQEQRFREEEEQRRERRQELQFLEEEEQLQRRERAQQLQEEDSFQE 505

Query: 379 EKHR 382

++ R Sbjct: 506 DRER 509

Score = 119 (17.9 bits), Expect = 2.2e-03, P = 2.2e-03 Identities = 79/357 (22%), Positives = 150/357 (42%)

Query: 33 KKWERPVAESLGHKDKDQEDYFQKGGLQIKFHCSKQLSLESSRQVTSESQEEPWEEEFGR 92

++ E+ + + K +++E Q+ + + +Q R+ + + + EE+F + Sbjct: 990 RREEQELRQERDRKFREEEQLLQE REEERLRRQERDRKFREEERQLRRQELEEQFRQ 1046

Query: 93 EMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNLEDLAREQQRRWVQLEKEQESPRR 152

E R+ LEE+ + Q++++K L QE K R+ E+ R +Q R QL +E++ R Sbjct: 1047 ERDRKFRLEEQ-IRQEKEEK-QLRRQERDRKFRE EEQQRRRQEREQQLRRERDRKFR 1101

Query: 153 EPEQLGEDVERRIFTPTSRWRDLEKAELSLVPAPSRTQSAHQSR--RPHLPMSPSTQQPA 210 E EQL ++ E R R L + E L+ + + R R + +++ Sb_jct: 1102 EEEQLLQEREEERLRRQERARKLREEE-QLLRREEQLLRQERDRKFREEEQLLQESEEER 1160

Query: 211 LGKQ RPMSSVEFTYRPRTRRVPTKPKKSASFPVTGTSIRRLTWPSLQISPANIKKKV 267

L +Q R + E + R + +++ +R+ Q ++++

Sbjct: 1161 LRRQERERKLREEEQLLQEREEERLRRQERARKLREEEQLLRQEEQELRQERARKLREEE 1220

Query: 268 YHMDMEAQ RKNLQLLS-EESELRLPHYLRSKALELTTTTMELGALRLQYL 316

+ E Q R+ QLL EE ELR + + E E LR Q

Sbjct: 1221 QLLRQEEQELRQERDRKFREEEQLLRREEQELRRERDRKFREEEQLLQEREEERLRRQER 1280

Query: 317 CHKYIFYRRLQSLRQEAINHVQIMKETEASYKAQNLYIFLENIDRLQ-SLRLQAWTDKQK 375

K + L E ++ +E + Y+A+ + E RL+ LR + +++ Sbjct: 1281 ARK—LREEEEQLLFEEQEEQRLRQERDRRYRAEEQFAREEKSRRLERELRQEEEQRRRR 1338

Query: 376 GLEEKHRE 383

E K RE Sbjct: 1339 ERERKFRE 1346

Score = 109 (16.4 bits), Expect = 1.9e-01, P = 1.7e-01 Identities = 37/113 (32%), Positives = 60/113 (53%)

Query: 67 KQLSLESSRQVTSESQ—EEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKL 124

+QL E R+ E Q +E EE R+ R + EEE++ Q+R+++ L QE + KL Sbjct: 764 QQLRRERDRKFREEEQLLQEREEERLRRQERERKLREEEQLLQEREEE-RLRRQERERKL 822

Query: 125 RQWNLEDLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAE 179

R+ E L +E++ ++ +E+E RE EQL E+ + R R L + E Sbjct: 823 REE—EQLLQEREEERLR-RQERERKLREEEQLLRQEEQEL—RQERARKLREEE 872

Score = 107 (16.1 bits), Expect = 3.0e-01, P = 2.6e-01 Identities = 35/109 (32%), Positives = 61/109 (55%)

Query: 71 LESSRQVTSESQEEPWE-EEFGREMRRQL WLEEEEMWQQRQKKWALLEQEHQEKLRQ 126

L Q+ ES+EE +E +++RR+ + EEE++ Q+R+++ L QE + KLR+ Sbjct: 742 LREEEQLLQESEEERLRRQEREQQLRRERDRKFREEEQLLQEREEE-RLRRQERERKLRE 800

Query: 127 WNLEDLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAE 179

E L +E++ ++ +E+E RE EQL ++ E R R L + E Sbjct: 801 E—EQLLQEREEERLR-RQERERKLREEEQLLQEREEERLRRQERERKLREEE 850

Score = 104 (15.6 bits), Expect = 9.4e-02, P = 9.0e-02 Identities = 84/339 (24%), Positives = 149/339 (43%)

Query: 67 KQLSLESSRQVTSESQEEPWEEEFGREMRRQL-WLEEEEMWQQRQKKWALLEQE—HQEK 123

+QL E ++ +EE EE RE R++L +LEEEE Q+R++ L E++ +++ Sbjct: 451 RQLRAEERQEQEQRFREE EEQRRERRQELQFLEEEEQLQRRERAQQLQEEDSFQEDR 507

Query: 124 LRQWNLEDLAREQQRRWVQLEKEQESPRR EP EQLGEDVE-RRIFTPTSRWRDL 175

R+ ++ Q RW QL++E + R +P EQL E+ E +R R R+ Sbjct: 508 ERRRRQQEQRPGQTWRW-QLQEEAQRRRHTLYAKPGQQEQLREEEELQREKRRQEREREY 566

Query: 176 EKAELSLVPAPSRTQSAHQSRRPHLPMSPSTQQPALGKQRPMSSVEFTYRPRT RRV 231

+ E L + + R + + Q+ L + R + E + R RR Sbjct: 567 REEE-KLQREEDEKRRRQERERQYRELEELRQEEQL-RDRKLREEEQLLQEREEERLRRQ 624

Query: 232 PTKPK KSASFPVTGTSIRRLTWPSLQISPANIKKKVYHMDMEAQRK NLQLLSEE 285

+ K + +R+ L+ ++++ + E +RK QLL E

Sbjct: 625 ERERKLREEEQLLRQEEQELRQERERKLREEEQLLRREEQELRQERERKLREEEQLLQER 684

Query: 286 SELRLPHYLRSKALE LTTTTMELGALRLQYLCHKYIFYRRL-QSLRQEAINHV— 337

E RL R++ L L EL R + L + RR Q LRQE + Sbjct: 685 EEERLRRQERARKLREEEQLLRQEEQELRQERERKLREEEQLLRREEQLLRQERDRKLRE 744

Query: 338 —QIMKETEASYKAQNLYIFLENIDRLQSLRLQAWTDKQKGLEEKHRECL 385

Q+++E+E + E +L+ R + + ++++ L+E+ E L Sbjct: 745 EEQLLQESEEERLRRQ EREQQLRRERDRKFREEEQLLQEREEERL 789

Score = 103 (15.5 bits), Expect = 1.2e-01, P = 1. le-01 Identities = 42/152 (27%), Positives = 74/152 (48%)

Query: 36 ERPVAESLGHKDKDQEDYFQKGGLQIKFHCSKQLSLESSRQVTSESQEEPWEEEFG-REM 94

ER + K +++E ++ +++ +++L E + + E QE E + RE Sbjct: 835 ERLRRQERERKLREEEQLLRQEEQELRQERARKLR-EEEQLLRQEEQELRQERDRKLREE 893

Query: 95 RRQLWLEEEEMWQQRQKKWA LLEQEHQEKLRQWNLEDLAREQQ RRWVQ-LEKE 146

+ L EE+E+ Q+R +K LL++ +E+LR+ E RE++ RR Q L +E Sbjct: 894 EQLLRQEEQELRQERDRKLREEEQLLQESEEERLRRQERERKLREEEQLLRREEQELRRE 953

Query: 147 QESPRREPEQLGEDVERRIFTPTSRWRDLEKAE 179 + RE EQL ++ E R R L + E Sbjct: 954 RARKLREEEQLLQEREEERLRRQERARKLREEE 986

Score = 103 (15.5 bits), Expect = 7.8e-01, P = 5.4e-01 Identities = 31/91 (34%), Positives = 52/91 (57%)

Query: 67 KQLSLESSRQVTSESQEEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQ 126

++L E R++ E Q EE+ R+ R + EEE++ Q+R+++ L QE KLR+ Sbjct: 642 QELRQERERKLREEEQLLRREEQELRQERERKLREEEQLLQEREEE-RLRRQERARKLRE 700

Query: 127 WNLEDLAREQQRRWVQLEKEQESPRREPEQL 157

E L R++++ +L +E+E RE EQL Sbjct: 701 E--EQLLRQEEQ ELRQERERKLREEEQL 726

Score = 101 (15.2 bits), Expect = 2.0e-01, P = 1.8e-01 Identities = 38/111 (34%), Positives = 57/111 (51%)

Query: 72 ESSRQVTSESQEEPWEE-EFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNLE 130

E R++ E Q EE E RE R+L EEE++ Q+R+++ L QE KLR+ + Sbjct: 931 ERERKLREEEQLLRREEQELRRERARKL-REEEQLLQEREEE-RLRRQERARKLREEE-Q 987

Query: 131 DLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAELSL 182

L RE+Q +L +E++ RE EQL ++ E R R + E L Sbjct: 988 LLRREEQ ELRQERDRKFREEEQLLQEREEERLRRQERDRKFREEERQL 1035

Score = 101 (15.2 bits), Expect = 1.3e+00, P = 7.2e-01 Identities = 33/108 (30%), Positives = 56/108 (51%)

Query: 72 ESSRQVTSEΞQEEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNLED 131

E R++ E Q EE+ R+ R + EEE++ +Q +++ L QE KLR+ E Sbjct: 841 ERERKLREEEQLLRQEEQELRQERARKLREEEQLLRQEEQE LRQERDRKLREE—EQ 895

Query: 132 LAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAE 179

L R++++ +L +E++ RE EQL ++ E R R L + E Sbjct: 896 LLRQEEQ ELRQERDRKLREEEQLLQESEEERLRRQERERKLREEE 940

Score = 99 (14.9 bits), Expect = 2.0e+00, P = 8.7e-01 Identities = 32/97 (32%), Positives = 50/97 (51%)

Query: 72 ESSRQVTSESQEEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNLED 131

E R+ E Q EE E R L EEE Q +++ L QE + KLR+ E Sbjct i 578 EKRRRQERERQYRELEELRQEEQLRDRKLREEEQLLQEREEERLRRQERERKLREE—EQ 635

Query: 132 LAREQ QRRWVQLEKEQESPRREPEQLGEDVERRI 165

L R++ Q R +L +E++ RRE ++L ++ ER++ Sbjct: 636 LLRQEEQELRQERERKLREEEQLLRREEQELRQERERKL 674

Score = 99 (14.9 bits), Expect = 2.0e+00, P = 8.7e-01 Identities = 34/111 (30%), Positives = 58/111 (52%)

Query: 67 KQLSLESSRQVTSESQ—EEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKL 124

++L E R++ E Q +E EE R+ R + EEE++ +Q +++ L QE + KL Sbjct: 664 QELRQERERKLREEEQLLQEREEERLRRQERARKLREEEQLLRQEEQE LRQERERKL 720

Query: 125 RQWNLEDLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEK 177

R+ + L RE+Q L +E++ RE EQL ++ E R + L + Sbjct: 721 REEE-QLLRREEQL LRQERDRKLREEEQLLQESEEERLRRQEREQQLRR 768

Score = 98 (14.7 bits), Expect = 2.6e+00, P = 9.2e-01 Identities = 37/146 (25%), Positives = 77/146 (52%)

Query: 20 EPVLLPLVDRRFPKKWERPVAESLGHKDKDQEDYFQKGGLQIKFHCSKQLSLESΞRQVTS 79

E LL ++ ++ ER + E + +E+ ++ K +QL + +++ Sbjct: 655 EEQLLRREEQELRQERERKLREEEQLLQEREEERLRRQERARKLREEEQLLRQEEQELRQ 714

Query: 80 ESQEEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNLED-LAREQQR 138

E + + EEE + +RR+ L +E ++ +++ LL++ +E+LR+ E L RE+ R Sbjct: 715 ERERKLREEE--QLLRREEQLLRQERDRKLREEEQLLQESEEERLRRQEREQQLRRERDR 772

Query: 139 RWVQLEKEQESPRREPEQLG-EDVERRI 165

++ E+EQ RE E+L ++ ER++ Sbjct: 773 KF—REEEQLLQEREEERLRRQERERKL 798

Score = 97 (14.6 bits), Expect = 3.3e+00, P = 9.6e-01 Identities = 38/129 (29%), Positives = 63/129 (48%)

Query: 72 ESSRQVTSESQ—EEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNL 129

E R++ E Q +E EE R+ R + EEE++ +Q +++ L QE KLR+ Sbjct: 817 ERERKLREEEQLLQEREEERLRRQERERKLREEEQLLRQEEQE LRQERARKLREE— 871

Query: 130 EDLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAELSLVPAPSRT 189 E L R++++ +L +E++ RE EQL E+ + R R L + E L+ Sbjct: 872 EQLLRQEEQ ELRQERDRKLREEEQLLRQEEQE —RQERDRKLREEE-QLLQESEEE 925

Query: 190 QSAHQSRRPHL 200

+ Q R L Sbjct: 926 RLRRQERERKL 936

Score = 96 (14.4 bits), Expect = 4.1e+00, P = 9.8e-01 Identities = 41/132 (31%), Positives = 69/132 (52%)

Query: 46 KDKDQEDYFQKGGLQI-KFHCSKQLSLEΞSRQVTSESQEEPWEEEFGREMRRQLWLEEEE 104

+++ QE F + Q+ + ++QL E S Q E + E+ G+ R QL +EE Sbjct: 473 RERRQELQFLEEEEQLQRRERAQQLQEEDSFQEDRERRRRQQEQRPGQTWRWQL QEE 529

Query: 105 MWQQRQKKWALLEQEHQEKLRQWNLEDLAREQQRRWVQLEKEQESPRREPEQLGEDVERR 164

++R +A Q QE+LR+ E+L RE++R+ E+E+E E Q ED +RR Sbjct: 530 AQRRRHTLYAKPGQ—QEQLREE—EELQREKRRQ EREREYREEEKLQREEDEKRR 581

Query: 165 IFTPTSRWRDLEK 177

++R+LE+ Sbjct: 582 RQERERQYRELEE 594

Score = 96 (14.4 bits), Expect = 4.1e+00, P = 9.8e-01 Identities = 35/138 (25%), Positives = 76/138 (55%)

Query: 28 DRRFPKKWERPVAESL-GHKDKDQEDYFQKGGLQIKFHCSKQLSLESSRQVTSESQEEPW 86

+R++ + E E L K +++E Q+ + ++ L Q+ + ++E Sbjct: 586 ERQYRELEELRQEEQLRDRKLREEEQLLQEREEERLRRQERERKLREEEQLLRQEEQE-L 644

Query: 87 EEEFGREMRRQLWL EEEEMWQQRQKKWALLEQEHQEKLRQWNLEDLAREQQRRWVQL 143

+E R++R + L EE+E+ Q+R++K L +E Q L++ E L R+++ R +L Sbjct: 645 RQERERKLREEEQLLRREEQELRQERERK LREEEQ-LLQEREEERLRRQERAR--KL 698

Query: 144 EKEQESPRREPEQLGEDVERRI 165

+E++ R+E ++L ++ ER++ Sbjct: 699 REEEQLLRQEEQELRQERERKL 720

Score = 95 (14.3 bits), Expect = 5.2e+00, P = 9.9e-01 Identities = 59/282 (20%), Positives = 121/282 (42%)

Query: 20 EPVLLPLVDRRFPKKWERPVAESLGHKDKDQEDYFQKGGLQIKFHCSKQLSLESSRQVTS 79

E LL ++ ++ ER + E + +E+ ++ K +QL + +++ Sbjct: 655 EEQLLRREEQELRQERERKLREEEQLLQEREEERLRRQERARKLREEEQLLRQEEQELRQ 714

Query: 80 ESQEEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNLED-LAREQQR 138

E + + EEE + +RR+ L +E ++ +++ LL++ +E+LR+ E L RE+ R Sbjct: 715 ERERKLREEE—QLLRREEQLLRQERDRKLREEEQLLQESEEERLRRQEREQQLRRERDR 772

Query: 139 RWVQLEKEQESPRREPEQLG-EDVERRIFTPTSRWRDLEKAELSLVPAPSRTQSAHQ—Ξ 195

++ E+EQ RE E+L ++ ER++ ++ E+ L + + Q Sbjct: 773 KF—REEEQLLQEREEERLRRQERERKLREEEQLLQEREEERLRRQERERKLREEEQLLQ 830

Query: 196 RRPHLPMSPSTQQPALGKQRPMSSVEFTYRPRTRRVPTKPKKSASFPVTGTSIRRLTWPS 255

R + ++ L ++ + E R R ++ +R+

Sbjct: 831 EREEERLRRQERERKLREEEQLLRQE-EQELRQERARKLREEEQLLRQEEQELRQERDRK 889

Query: 256 LQISPANIKKKVYHMDMEAQRK NLQLLΞEESELRLPHYLRSKAL 299

L+ ++++ + E RK QLL E E RL R + L Sbjct: 890 LREEEQLLRQEEQELRQERDRKLREEEQLLQESEEERLRRQERERKL 936

Score = 94 (14.1 bits), Expect = l.le+00, P = 6.8e-01 Identities = 35/116 (30%), Positives = 59/116 (50%)

Query: 72 ESSRQVTSESQEEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEK L 124

E +R++ E Q EE+ R+ R + + EEE++ Q+R+++ L QE K L Sbjct: 977 ERARKLREEEQLLRREEQELRQERDRKFREEEQLLQEREEE-RLRRQERDRKFREEERQL 1035

Query: 125 RQWNLEDLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAELSL 182

R+ LE+ R+++ R +LE EQ +E +QL R F + R ++ E L Sbjct: 1036 RRQELEEQFRQERDRKFRLE-EQIRQEKEEKQLRRQERDRKFREEEQQRRRQEREQQL 1092

Score = 94 (14.1 bits), Expect = l.le+00, P = 6.8e-01 Identities = 51/166 (30%), Positives = 76/166 (45%)

Query: 67 KQLSLESSRQVTSESQ—EEPWEEEFGREMR-RQLWLEEEEMWQQRQKKWALLEQEHQEK 123

++L E R+ E Q +E EE R+ R R+L EEE++ + Q++ L QE+ Sbjct: 1250 QELRRERDRKFREEEQLLQEREEERLRRQERARKLREEEEQLLFEEQEEQRL RQER 1305

Query: 124 LRQWNLED-LAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAELSL 182

R++ E+ ARE++ R +LE+E R+E EQ R F R E+ E Sbjct: 1306 DRRYRAEEQFAREEKSR—RLEREL RQEEEQRRRRERERKFREEQLRRQQEE-EQRR 1359 Query: 183 VPAPSRTQSAHQSRRPHLPMSPSTQQPALGKQRPMSSVEFTYRPRTRRVP 232

R QSRR L P T+Q A R E+ R++ P Sb_jct: 1360 RQLRERQFREDQSRRQVL--EPGTRQFARVPVRSSPLYEYIQEQRSQYRP 1407

Score = 93 (14.0 bits), Expect = 8.3e+00, P = 1. Oe+00 Identities = 41/145 (28%), Positives = 72/145 (49%)

Query: 28 DRRFPKKWERPVAESLGHKDKDQEDYFQKGGLQIKFHCSKQLSLESSRQVTSESQEEPW- 86

+RR ++ ER + E + + Q + + Q + L R + QE+ + Sbjct: 408 ERRQRQERERELEEQARRQQQWQAEEESERRRQ-RLSARPSLRERQLRAEERQEQEQRFR 466

Query: 87 -EEEFGREMRRQL-WLEEEEMWQQRQKKWALLEQE—HQEKLRQWNLEDLAREQQRRWVQ 142

EEE RE R++L +LEEEE Q+R++ L E++ +++ R+ ++ Q RW Q Sbjct: 467 EEEEQRRERRQELQFLEEEEQLQRRERAQQLQEEDSFQEDRERRRRQQEQRPGQTWRW-Q 525

Query: 143 LEKEQESPRR EP EQLGEDVE 162

L++E + R +P EQL E+ E Sbjct: 526 LQEEAQRRRHTLYAKPGQQEQLREEEE 552

Score = 91 (13.7 bits), Expect = 2.4e+00, P = 9. le-01 Identities = 38/110 (34%), Positives = 57/110 (51%)

Query: 72 ESSRQVTSESQEEPWEE-EFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNL- 129

E R++ E Q EE E RE R+L EEE++ Q+R+++ L QE KLR+ Sbjct: 931 ERERKLREEEQLLRREEQELRRERARKL-REEEQLLQEREEE-RLRRQERARKLREEEQL 988

Query: 130 EDLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAEL 180

++L +E+ R++ E+EQ RE E+L R F R L + EL Sbjct: 989 LRREEQELRQERDRKF--REEEQLLQEREEERLRRQERDRKFREEER--QLRRQEL 1040

Score = 89 (13.4 bits), Expect = 2.2e+00, P = 8.9e-01 Identities = 35/138 (25%), Positives = 65/138 (47%)

Query: 82 QEEPWEEEFGREMRRQLWLEEEEM—WQQRQKKWALLEQEHQEKLRQWNLEDLAREQQRR 139

Q E++ E+R + + +E E WQ+++++ L E+E Q K R+ + +R+ + + Sbjct: 111 QNRRQEDQRRFELRDRQFEDEPERRRWQKQEQERELAEEEEQRKKRERFEQHYSRQYRDK 170

Query: 140 WVQLEKEQ-ESPRREPEQL GEDVERRIFTPTSRWRDLEKAELSLVPAPSRTQSAHQ 194

+L++++ E R E EQL G D E F + R E+ EL Q +

Sbjct: 171 EQRLQRQELEERRAEEEQLRRRKGRDAEE--FIEEEQLRRREQQELKR-ELREEEQQRRE 227

Query: 195 SRRPHLPMSPΞTQQPALGKQR 215

R H ++ L ++R Sbjct: 228 RREQHERALQEEEEQLLRQRR 248

Score = 50 (7.5 bits), Expect = 2.2e+00, P = 8.9e-01 Identities = 34/160 (21%), Positives = 67/160 (41%)

Query: 325 RLQSLRQEAINHVQIMKETEASYKAQNLYIFLENIDRL-QSLRLQAWTDKQKGLEEKHRE 383

R + R+E Q+ +E E + + LE +R Q LR + ++++ E++ R Sbjct: 245 RQRRWREEPREQQQLRRELEEIREREQR LEQEERREQQLRREQRLEQEERREQQLRR 301

Query: 384 CLSSMVTMFPKLQLEWNVHLNIP-EVTSPKPKKCKLPAASPRHIRPΞGPTYKQPFLSRHR 442

L + +L+ E + E + K +L R R ++ L+ Sbjct: 302 ELEEIREREQRLEQEERREQRLEQEERREQQLKRELEEIREREQRLEQEERREQLLAEEV 361

Query: 443 ACVPLQMARQQGKQMEAVWKTEVASSSYAIEKKTPASLPRDQ 484

+ AR++G+ + W+ ++ S + A + K S PR Q Sbjct: 362 R EQARERGESLTRRWQRQLESEAGARQSKV-YΞRPRRQ 398

Score = 40 (6.0 bits), Expect = 1.9e-01, P = 1.7e-01 Identities = 32/115 (27%), Positives = 47/115 (40%)

Query: 276 RKNLQLLSEESELRLPHYLRSKAL—ELTTTTMELGALRLQYLCHKYIFYRRL-QSLRQE 332

R+ QLL E E RL R++ L E E LR Q K+ +L Q +E Sbjct: 959 REEEQLLQEREEERLRRQERARKLREEEQLLRREEQELR-QERDRKFREEEQLLQEREEE 1017

Query: 333 AINHVQI MKETEASYKAQNLYI-FLENIDRLQSLRLQAWTDKQ-KGLEEKHRE 383

+ + +E E + Q L F + DR L Q +K+ K L + R+ Sbjct: 1018 RLRRQERDRKFREEERQLRRQELEEQFRQERDRKFRLEEQIRQEKEEKQLRRQERD 1073

Score = 37 (5.6 bits), Expect = 1.6e+00, P = 7.9e-01 Identities = 27/108 (25%), Positives = 43/108 (39%)

Query: 276 RKNLQLLSEESELRLPHYLRSKAL ELTTTTMELGALRLQYLCHKYIFYRRLQSLRQE 332

R+ QLL E E RL R + L E E LR Q K R + L QE Sbjct: 775 REEEQLLQEREEERLRRQERERKLREEEQLLQEREEERLRRQERERKL REEEQLLQE 831

Query: 333 AINHVQIMKETEASYKAQNLYIFLENIDRLQSLRLQAWTDKQKGLEEKHRE 383

+E E + + + E L+ R + ++++ L ++ +E Sbjct: 832 REEERLRRQERERKLREEEQLLRQEE-QELRQERARKLREEEQLLRQEEQE 881 Pedant information for DKFZphtes3_50n23 , frame 1

Report for DKFZphtes3_50n23. 1

[LENGTH] 499

[MW] 58885 . 69

[pi ] 9 . 67

[KW] All_Alpha

[KW] LOW_COMPLEXITY 10.42 %

SEQ MTVRSRVADVFGΞKDTESLEPVLLPLVDRRFPKKWERPVAESLGHKDKDQEDYFQKGGLQ SEG

PRD ccccccceeecccccccccceeeccccccccccccchhhhhhhcccccccccccccccce

SEQ IKFHCSKQLSLESSRQVTSESQEEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEH

SEG xxxxxxxxxx..xxxxxxxxxxxxxxxxxxx

PRD eeeecchhhhhhccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ QEKLRQWNLEDLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAEL SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccccccceeeccccccchhhhhhhh

SEQ SLVPAPSRTQSAHQSRRPHLPMSPSTQQPALGKQRPMSSVEFTYRPRTRRVPTKPKKΞAS

SEG xxxxxxxxxxxxxxx ...

PRD hccccccchhhhhccccccccccccccccccccccccceeeeeeccccccccccccceee

SEQ FPVTGTSIRRLTWPSLQISPANIKKKVYHMDMEAQRKNLQLLSEESELRLPHYLRSKALE SEG xxxxxxxx

PRD ecccccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ LTTTTMELGALRLQYLCHKYIFYRRLQSLRQEAINHVQIMKETEASYKAQNLYIFLENID SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ RLQSLRLQAWTDKQKGLEEKHRECLSSMVTMFPKLQLEWNVHLNIPEVTSPKPKKCKLPA SEG

PRD hhhhhhhhhhhhcchhhhhhhhhhhhhhhhccccchhhhhcccccccccccccccccccc

SEQ ASPRHIRPΞGPTYKQPFLSRHRACVPLQMARQQGKQMEAVWKTEVASSSYAIEKKTPASL SEG

PRD ccccccccccccccchhhhhhccchhhhhhhhhcchhhhhhhhhhhhhhhhhhhcccccc

SEQ PRDQLRGHPDIPRLLTLDV SEG

PRD ccccccccccccccccccc

(No Prosite data available for DKFZphtes3_50n23.1) (No Pfam data available for DKFZphtes3_50n23.1)

DKFZphtes3_6b21

group: testes derived

DKFZphtes3_6b21 encodes a novel 781 amino acid protein without similarity to human KIAA0256 gene product.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . similarity to KIAA0256 complete cDNA, complete eds, EST hits

Sequenced by BMFZ

Locus: /map="356.3 cR from top of Chr9 linkage group"

Insert length: 3360 bp

Poly A stretch at pos. 3314, polyadenylation signal at pos. 3300

1 GGCAAGCCGA CGGCCCGCTG CTGGCCTCCG TGACGCGGCC TCCTCCGCGC 51 CTCGCGGCAT GGCGTCGGAG GGGCCGCGGG AGCCCGAAAG CGAGGGCATC

101 AAGTTATCAG CAGATGTCAA ACCATTTGTC CCCAGATTTG CCGGGCTCAA

151 TGTGGCATGG TTAGAGTCCT CAGAAGCATG TGTCTTCCCC AGCTCTGCAG

201 CCACATACTA TCCGTTTGTT CAGGAACCAC CAGTGACAGA AATGTTTACT

251 CAGTGCCTGG CTCCCAGTAT CTTTATAACC AACCCAGTTG TTACCGAGGT

301 TTTCAAACAG TGAAGCATCG AAATGAGAAC ACATGCCCTC TCCCACAAGA

351 AATGAAAGCT CTGTTTAAGA AGAAAACCTA TGATGAGAAA AAAACGTATG

401 ATCAGCAAAA GTTTGACAGT GAAAGGGCTG ATGGAACTAT ATCATCTGAG

451 ATAAAATCAG CTAGAGGTTC ACATCATTTG TCCATTTACG CTGAGAATAG

501 TTTGAAATCA GATGGTTACC ATAAGCGAAC AGACAGGAAA TCCAGAATCA

551 TTGCAAAAAA TGTATCTACC TCCAAACCTG AGTTTGAATT TACCACACTG

601 GACTTTCCTG AACTGCAAGG TGCAGAGAAC AATATGTCAG AGATACAGAA

651 GCAACCCAAG TGGGGACCTG TCCACTCTGT CTCTACCGAC ATTTCTCTTC

701 TAAGAGAAGT AGTAAAACCA GCTGCAGTGT TATCAAAGGG TGAAATAGTG

751 GTGAAAAATA ACCCAAATGA ATCTGTAACT GCTAATGCCG CTACCAATTC

801 TCCTTCATGT ACAAGAGAGT TATCTTGGAC ACCAATGGGT TATGTTGTTC

851 GACAGACATT ATCTACAGAA CTGTCAGCAG CCCCTAAAAA TGTTACTTCT

901 ATGATAAACT TAAAGACCAT TGCTTCATCA GCAGATCCTA AAAATGTTAG

951 TATACCATCT TCTGAAGCTT TATCTTCGGA TCCTTCCTAC AACAAAGAAA 1001 AACACATTAT TCATCCTACC CAAAAGTCTA AAGCATCACA AGGTAGTGAC 1051 CTTGAACAAA ATGAAGCCTC AAGAAAGAAT AAGAAAAAGA AAGAAAAATC 1101 TACATCAAAA TATGAAGTCC TGACAGTTCA AGAGCCTCCA AGGATTGAAG 1151 ATGCCGAGGA ATTTCCCAAC CTGGCAGTTG CATCTGAAAG AAGAGACAGA 1201 ATAGAGACAC CGAAATTTCA ATCTAAGCAG CAGCCACAGG ATAATTTTAA 1251 AAATAATGTA AAGAAGAGCC AGCTTCCAGT GCAGTTGGAC TTGGGGGGCA 1301 TGCTGACAGC CCTGGAGAAG AAGCAGCACT CTCAGCATGC AAAGCAGTCC 1351 TCCAAACCAG TGGTAGTCTC AGTTGGAGCA GTGCCAGTCC TTTCCAAAGA 1401 ATGTGCATCA GGGGAGAGAG GCCGCCGCAT GAGTCAAATG AAGACCCCGC 1451 ACAATCCCTT GGACTCCAGC GCCCCACTGA TGAAGAAAGG GAAGCAGAGG 1501 GAGATCCCCA AGGCCAAGAA GCCAACCTCA CTGAAGAAGA TTATTTTGAA 1551 AGAACGGCAA GAGAGAAAGC AGCGTCTCCA AGAAAATGCT GTGAGTCCAG 1601 CTTTTACCAG TGATGACACA CAAGATGGAG AGAGTGGTGG TGATGACCAG 1651 TTTCCCGAGC AGGCAGAGCT GTCAGGGCCA GAGGGGATGG ACGAACTGAT 1701 CTCCACTCCT TCGGTTGAGG ACAAGTCTGA AGAGCCACCA GGCACAGAGC 1751 TCCAGAGGGA CACAGAGGCC TCCCACCTTG CTCCCAATCA CACCACCTTC 1801 CCTAAGATCC ACAGCCGCAG ATTCAGGGAT TACTGCAGCC AGATGCTTAG 1851 TAAAGAAGTG GATGCTTGTG TTACCGACCT ACTCAAAGAA CTGGTCCGTT 1901 TCCAAGACCG TATGTACCAG AAAGATCCAG TCAAGGCCAA GACTAAACGT 1951 CGACTTGTGT TGGGGTTGAG GGAGGTTCTC AAACACCTGA AGCTCAAAAA 2001 ACTGAAATGT GTCATTATTT CTCCCAACTG TGAGAAGATA CAGTCAAAAG 2051 GTGGGCTGGA TGACACTTTG CACACAATTA TTGATTATGC CTGTGAGCAG 2101 AACATTCCCT TTGTGTTTGC TCTCAACCGC AAAGCTCTGG GGCGCAGTTT 2151 GAATAAGGCA GTTCCTGTCA GTGTGGTGGG GATCTTCAGC TATGATGGGG 2201 CCCAGGATCA GTTCCACAAG ATGGTTGAGC TGACAGTGGC GGCCCGACAG 2251 GCGTACAAGA CCATGCTGGA GAATGTGCAG CAGGAGCTGG TGGGAGAGCC 2301 CAGGCCTCAG GCACCTCCCA GCCTACCCAC ACAGGGCCCC AGCTGCCCTG 2351 CAGAAGATGG CCCCCCAGCC CTGAAAGAAA AAGAAGAGCC ACACTACATT 2401 GAAATCTGGA AAAAACATCT GGAAGCATAC AGTGGATGTA CCCTGGAGCT 2451 AGAAGAATCC TTGGAGGCTT CAACCTCTCA AATGATGAAT TTGAATTTAT 2501 GAGAGTTCTT GCCTGTGTGT CTGTATTTTG GGTAAGGAGG GGAGGTCTGA 2551 AAAAGACTTT GGGGCTTTTT CTTCTGTTTT TCATGACAAT GTAATTTGTG 2601 TAACTGTTGA ATCTGGAAAT TGATCAGCAT TAAAGGGCAC ATGAAGCAGT 2651 GTCTGCAGGC GTTCAGTGCT GCGGAGCCTG TTAAAGGTCA CTCAGATGTG 2701 CAGGTGTTAA TCTTCTCTAA AAGCCTGGTT ATACAGCTCT GGCTTTCTGA

2751 GCACACTACG GATCTGGAAA ATACTGGAAA ATGTGATACT TAGAATACTT

2801 TGGCTGCTAA GGAAACTTCC TCTCCATTGC AGAATAGCTG AGCCAAGTGA

2851 GTGAGTTTGC AGAAAGCAGG TGGTGAGCTC CTGCCTGCTG GAGGTTGCCA

2901 TGGAGGGCCA TTCCTGCCCG GCAACAGCAC CGTCCTGCAG GGAGCCACTT

2951 GGCAGAAGGG TGCAGGGCTG CTGGTGTCAG AGCAAGAGGG CTACAGGGAA

3001 AGGGCCCTTT CTCAGGGGAT GTAGCTTTTT TAAAAGATTT GGGAACACTT

3051 GGAGGATTTG CTAAAATGAG CCTCAGAAGG AAAATTGGTT TTCTAACCTG

3101 TGACTTTTTG AAATGAATTA TTCCTTTCAG TCTTTATTTT TCAAAGAAAC

3151 AATGTGTATT GAAGTACCTA GATTTGTTTG ATAATCAACA AATCTTTCCT

3201 TTTTCAATGA ACATATTCTG AATGTGGTTT CTGTCTTAGA CCAGGAGGAC

3251 AGAGTTTGCT TTCATATTTT CCCTGTAAGT AAGAGGGCTT ATTTATTTTA

3301 AATAAAGAGT AATTATTAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

3351 AAAAAAAAAA

BLAST Results

Entry HS773347 from database EMBL: human STS WI-18160. Score = 813, P = 2.9e-30, identities = 167/171

Medlme entries

No Medline entry

Peptide information for frame 1

ORF from 157 bp to 2499 bp; peptide length: 781 Category: similarity to known protein

1 MVRVLRSMCL PQLCSHILSV CSGTTSDRNV YΞVPGSQYLY NQPSCYRGFQ

51 TVKHRNENTC PLPQEMKALF KKKTYDEKKT YDQQKFDSER ADGTISSEIK

101 SARGSHHLΞI YAENSLKSDG YHKRTDRKSR IIAKNVSTSK PEFEFTTLDF

151 PELQGAENNM SEIQKQPKWG PVHSVSTDIS LLRE VKPAA VLSKGEIVVK

201 NNPNESVTAN AATNSPSCTR ELSWTPMGYV VRQTLΞTELS AAPKNVTSMI

251 NLKTIASSAD PKNVSIPSSE ALSΞDPSYNK EKHIIHPTQK SKASQGSDLE

301 QNEASRKNKK KKEKSTSKYE VLTVQEPPRI EDAEEFPNLA VASERRDRIE

351 TPKFQSKQQP QDNFKNNVKK SQLPVQLDLG GMLTALEKKQ HSQHAKQSSK

401 PVVVSVGAVP VLSKECASGE RGRRMSQMKT PHNPLDSSAP LMKKGKQREI

451 PKAKKPTSLK KIILKERQER KQRLQENAVS PAFTSDDTQD GESGGDDQFP

501 EQAELSGPEG MDELISTPSV EDKSEEPPGT ELQRDTEASH LAPNHTTFPK

551 IHSRRFRDYC SQMLSKEVDA CVTDLLKELV RFQDRMYQKD PVKAKTKRRL

601 VLGLREVLKH LKLKKLKCVI ISPNCEKIQS KGGLDDTLHT IIDYACEQNI

651 PFVFALNRKA LGRSLNKAVP VSVVGIFSYD GAQDQFHKMV ELTVAARQAY

701 KTMLENVQQE LVGEPRPQAP PSLPTQGPSC PAEDGPPALK EKEEPHYIEI

751 WKKHLEAYSG CTLELEESLE ASTSQMMNLN L

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_6b21, frame 1

SWISSPROT :Y256_HUMAN HYPOTHETICAL PROTEIN KIAA0256., N = 1, Score = 786, P = 3.6e-78

TREMBL :PFMAL3P3_15 gene: "MAL3P3.15"; Plasmodium falciparum MAL3P3, N = 2, Score = 161, P = 5. le-10

TREMBL :RNNFLH_1 Rat heavy neurofilament subunit (NF-H) mRNA, 3' end., N = 1, Score = 150, P = 9. le-07

>SWISSPROT:Y256_HUMAN HYPOTHETICAL PROTEIN KIAA0256. Length = 635

HSPs:

Score = 786 (117.9 bits), Expect = 3.6e-78, P = 3.6e-78 Identities = 190/424 (44%), Positives = 263/424 (62%) Query: 369 KKSQLPVQLDLGGMLTALEKKQHΞQHAKQ—SSKPVVVSVGAVPVLSKECASGERGRRMS 426

KK++ PVQLDLG ML ALEK+Q + A+Q +++P+ +V + ++ + S Sbjct: 16 KKNKTPVQLDLGDMLAALEKQQQAMKARQITNTRPLSYTVVTAASFHTKDSTNRKPLTKS 75

Query: 427 Q-MKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVSPAFTS 485

Q T N +D ++ KKGK++EI K K+PT+LKK+ILKER+E+K RL + S Sbjct: 76 QPCLTSFNSVDIASSKAKKGKEKEIAKLKRPTALKKVILKEREEKKGRLTVD—HNLLGS 133

Query: 486 DDTQDGEΞGGDDQFPEQAELSGPEGMDELISTPSVEDKSEEPPG—TELQRDTEASHL— 541

++ + D P++ G+ + S S+ S+ P T + + + AS Sbjct: 134 EEPTEMHLDFIDDLPQEIVSQEDTGLS-MPSDTSLSPASQNSPYCMTPVSQGSPASSGIG 192

Query: 542 APN-HTTFPKIHSRRFRDYCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRL 600

+P +T KIHS+RFR+YC+Q+L KE+D CVT LL+ELV FQ+R+YQKDPV+AK +RRL Sbjct: 193 SPMASSTITKIHSKRFREYCNQVLCKEIDECVTLLLQELVSFQERIYQKDPVRAKARRRL 252

Query: 601 VLGLREVLKHLKLKKLKCVIISPNCEKIQSKGGLDDTLHTIIDYACEQNIPFVFALNRKA 660

V+GLREV KH+KL K+KCVIISPNCEKIQSKGGLD+ L+ +1 A EQ IPFVFAL RKA Sbjct: 253 VMGLREVTKHMKLNKIKCVIISPNCEKIQSKGGLDEALYNVIAMAREQEIPFVFALGRKA 312

Query: 661 LGRSLNKAVPVSVVGIFSYDGAQDQFHKMVELTVAARQAYKTMLENVQQELVGEPRP 717

LGR +NK VPVSVVGIF+Y GA+ F+K+VELT AR+AYK M+ ++QE E Sbjct: 313 LGRCVNKLVPVSVVGIFNYFGAESLFNKLVELTEEARKAYKDMVAAMEQEQAEEALKNVK 372

Query: 718 QAPPSLP-TQGPS CPAEDGPPALKEKEEPHYIEIWKKHLEAYSGCTL ELE 766

+ P + ++ PS C P + E E Y W+ +E G E E Sbjct: 373 KVPHHMGHSRNPSAASAISFCSVISEP—IΞEVNEKEYETNWRNMVETSDGLEAΞENEKE 430

Query: 767 ESLEASTSQ 775

S + STS+ Sbjct: 431 VSCKHSTSE 439

Pedant information for DKFZphtes3_6b21, frame 1

Report for DKFZphtes3_6b21.1

[LENGTH] 781

[MW] 87393.44

[pi] 8.94

[HOMOL] SWISSPROT :Y256_HUMAN HYPOTHETICAL PROTEIN KIAA0256. 4e-75

[PROSITE] MYRISTYL 4

[PROSITE] AMIDATION 1

[PROSITE] CAMP_PHOSPHO_SITE 3

[PROSITE] CK2_PHOSPHO_SITE 16

[PROSITE] TYR_PHOSPHO_SITE 4

[PROSITE] PKC_PHOSPHO_SITE 16

[PROSITE] ASN_GLYCOSYLATION 6

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 8.45 %

SEQ MVRVLRSMCLPQLCSHILSVCΞGTTSDRNVYSVPGSQYLYNQPSCYRGFQTVKHRNENTC SEG PRD ccceeeeeccceeeeeeeeeeccccccccccccccccccccccceeeceeeeeecccccc

SEQ PLPQEMKALFKKKTYDEKKTYDQQKFDSERADGTISSEIKSARGSHHLSIYAENSLKSDG SEG xxxxxxxxxxxx PRD cccchhhhhhhhhhccchhhhhhhhhhhccccccchhhhhhhcccceeeeeeeecccccc

SEQ YHKRTDRKSRIIAKNVSTSKPEFEFTTLDFPELQGAENNMSEIQKQPKWGPVHSVSTDIS SEG PRD cccccchhhhheeeccccccccceeecccccccccccchhhhhhccccccccceeecchh

SEQ LLREVVKPAAVLSKGEIVVKNNPNESVTANAATNSPSCTRELSWTPMGYVVRQTLΞTELS SEG PRD hhhhhhheeeeecccceeeeccccceeeeeecccccccceeeeeccceeeeeeccccccc

SEQ AAPKNVTSMINLKTIASSADPKNVSIPSSEALSSDPSYNKEKHIIHPTQKSKASQGSDLE SEG PRD ccccceeeeehhhhhhcccccceeeecccccccccccccccceeechhhhhhhcccccch

SEQ QNEAΞRKNKKKKEKSTSKYEVLTVQEPPRIEDAEEFPNLAVASERRDRIETPKFQSKQQP

SEG .... xxxxxxxxxxxxxx

PRD hhhhccccccccccccceeeeeecccccchhhhhhccchhhhhhhhhhhhcccccccccc

SEQ QDNFKNNVKKSQLPVQLDLGGMLTALEKKQHSQHAKQSSKPVVVSVGAVPVLSKECASGE

SEG xxxxxxxxxxxxxxxxx

PRD cccccccccccccccccccchhhhhhhhhhhhhhhhhhhccceeeeeeeeeeeecccccc

PS00008 633->639 MYRISTYL PDOC00008

PS00009 421->425 AMIDATION PDOC00009

(No Pfam data available for DKFZphtes3_6b21.1)

DKFZphtes3_6cll

group: signal transduction

DKFZphtes3_6cll encodes a novel 1025 amino acid protein with similarity to A. ambisexualis antheridiol steroid receptor.

The novel protein is a putative steroid receptor. It shares similarity with yeast YNL132w and contains the ATP/GTP-bindmg site motif A (P-loop) and RGD site, similar to the A. ambisexualis antheridiol steroid receptor.

The new protein can find application in modulating/blockmg the expression of genes controlled by this receptor. strong similarity to YNL132w strong similarity to S. pombe/YDK9_SCHPO, S . cerevιsιae/YNL132w, C.elegans/F55A12.8

Sequenced by BMFZ

Locus : unknown

Insert length: 3966 bp

Poly A stretch at pos. 3890, polyadenylation signal at pos. 3873

1 GCTGTGCCTT CTCTTTCGGA GTTGTTCCGT GCTCCCACGT GCTTCCCCTT 51 CTCCACTGGC TGGGATCCCC CGGGCTCGGG GCGCAGTAAT AATTTTTCAC

101 CATGCATCGG AAAAAGGTGG ATAACCGAAT CCGGATTCTC ATTGAGAATG

151 GAGTAGCTGA GCGGCAAAGA TCTCTCTTTG TTGTAGTTGG GGATCGAGGA

201 AAAGATCAGG TGGTAATACT TCATCACATG TTATCCAAAG CAACTGTGAA

251 GGCTCGGCCT TCAGTGCTGT GGTGTTATAA GAAAGAGCTG GGGTTTAGCA

301 GTCACCGGAA GAAAAGAATG CGACAGCTGC AGAAGAAAAT AAAGAATGGA

351 ACACTGAACA TAAAGCAGGA CGACCCCTTT GAACTCTTCA TAGCAGCCAC

401 AAACATTCGC TACTGCTACT ACAACGAGAC CCACAAGATC CTGGGCAATA

451 CCTTCGGCAT GTGTGTGCTG CAGGATTTTG AAGCCTTAAC TCCAAACTTG

501 CTGGCCAGGA CTGTAGAAAC AGTGGAAGGT GGTGGGCTAG TGGTCATCCT

551 CCTACGGACC ATGAACTCAC TCAAGCAATT GTACACAGTG ACTATGGATG

601 TGCATTCCAG GTACAGAACT GAGGCCCATC AGGATGTGGT GGGAAGATTT

651 AATGAAAGGT TTATTCTGTC TCTGGCCTCT TGTAAGAAGT GTCTCGTCAT

701 TGATGACCAG CTCAACATCC TGCCCATCTC CTCCCACGTT GCCACCATGG

751 AGGCCCTGCC TCCCCAGACT CCGGATGAGA GTCTTGGTCC TTCTGATCTG

801 GAGCTGAGGG AGTTGAAGGA GAGCTTGCAG GACACCCAGC CTGTGGGTGT

851 GTTGGTGGAC TGCTGTAAGA CTCTAGACCA GGCCAAAGCT GTCTTGAAAT

901 TTATCGAGGG CATCTCTGAA AAGACCCTGA GGAGTACTGT TGCACTCACA

951 GCTGCTCGAG GACGGGGAAA ATCTGCAGCC CTGGGATTGG CGATTGCTGG 1001 GGCGGTGGCA TTTGGGTACT CCAATATCTT TGTTACCTCC CCAAGCCCTG 1051 ATAACCTCCA TACTCTGTTT GAATTTGTAT TTAAAGGATT TGATGCTCTG 1101 CAATATCAGG AACATCTGGA TTATGAGATT ATCCAGTCTC TAAATCCTGA 1151 ATTTAACAAA GCAGTGATCA GAGTGAATGT ATTTCGAGAA CACAGGCAGA 1201 CTATTCAGTA TATACATCCT GCAGATGCTG TGAAGCTGGG CCAGGCTGAA 1251 CTAGTTGTGA TTGATGAAGC TGCCGCCATC CCCCTCCCCT TGGTGAAGAG 1301 CCTACTTGGC CCCTACCTTG TTTTCATGGC ATCCACCATC AATGGCTATG 1351 AGGGCACTGG CCGGTCACTG TCCCTCAAGC TAATTCAGCA GCTCCGTCAA 1401 CAGAGCGCCC AGAGCCAGGT CAGCACCACT GCTGAGAATA AGACCACGAC 1451 GACAGCCAGA TTGGCATCAG CGCGGACACT GCATGAGGTT TCCCTCCAGG 1501 AGTCAATCCG ATACGCCCCT GGGGATGCAG TGGAGAAGTG GCTGAATGAC 1551 TTGCTGTGCC TGGATTGCCT CAACATCACT CGGATAGTCT CAGGCTGCCC 1601 CTTGCCTGAA GCTTGTGAAC TGTACTATGT TAATAGAGAT ACCCTCTTTT 1651 GCTACCACAA GGCCTCTGAA GTTTTCCTCC AACGGCTTAT GGCCCTCTAC 1701 GTGGCTTCTC ACTACAAGAA CTCTCCCAAT GATCTCCAGA TGCTCTCCGA 1751 TGCACCTGCT CACCATCTCT TCTGCCTTCT GCCTCCTGTG CCCCCCACCC 1801 AGAATGCCCT TCCAGAAGTG CTTGCTGTTA TCCAGGTGTG CCTTGAAGGG 1851 GAGATTTCTC GCCAGTCCAT CTTGAACAGT CTGTCTCGAG GCAAGAAGGC 1901 TTCAGGGGAC CTGATTCCAT GGACAGTGTC AGAACAGTTC CAAGATCCAG 1951 ACTTTGGTGG TCTGTCTGGT GGAAGGGTCG TTCGCATTGC TGTTCACCCA 2001 GATTATCAAG GGATGGGCTA TGGCAGCCGT GCTCTGCAGC TGCTGCAGAT 2051 GTACTATGAA GGCAGGTTTC CTTGTCTGGA GGAAAAGGTC CTTGAGACAC 2101 CACAGGAAAT TCACACCGTA AGCAGCGAGG CTGTCAGCTT GTTGGAAGAG 2151 GTCATCACTC CCCGGAAGGA CCTGCCTCCT TTACTCCTCA AATTGAATGA 2201 GAGGCCTGCC GAACGCCTGG ATTACCTGGG TGTTTCCTAT GGCTTGACCC 2251 CCAGGCTCCT CAAGTTCTGG AAACGAGCTG GATTTGTTCC TGTTTATCTG 2301 AGACAGACCC CGAATGACCT GACCGGAGAG CACTCGTGCA TCATGCTGAA 2351 GACGCTCACT GATGAGGATG AGGCTGACCA GGGAGGCTGG CTTGCAGCCT 2401 TCTGGAAAGA TTTCCGACGG CGGTTCCTAG CCTTGCTCTC CTACCAGTTC 2451 AGTACCTTCT CTCCTTCCCT GGCTCTGAAC ATCATTCAGA ACAGGAACAT 2501 GGGGAAGCCA GCCCAGCCTG CCCTGAGCCG GGAGGAGCTG GAAGCACTCT 2551 TCCTCCCCTA TGACCTGAAG CGGCTGGAGA TGTATTCACG GAATATGGTG

2601 GACTATCACC TCATCATGGA CATGATCCCG GCCATCTCTC GCATCTATTT

2651 CCTGAACCAG CTGGGGGACC TGGCCCTGTC TGCGGCTCAG TCGGCTCTTC 2701 TCTTGGGGAT TGGCCTGCAG CATAAGTCTG TGGACCAGCT GGAAAAGGAG 2751 ATTGAGCTGC CCTCGGGCCA GTTGATGGGA CTTTTCAACC GGATCATCCG 2801 CAAAGTTGTG AAGCTATTTA ATGAAGTTCA GGAAAAGGCC ATTGAGGAGC 2851 AGATGGTGGC AGCGAAGGAT GTGGTCATGG AGCCCACGAT GAAGACCCTC 2901 AGTGACGACC TAGATGAAGC AGCAAAGGAA TTTCAGGAGA AACACAAGAA 2951 GGAAGTAGGG AAGCTGAAGA GCATGGACCT CTCTGAATAC ATAATCCGTG 3001 GGGACGATGA AGAGTGGAAT GAAGTTTTGA ACAAAGCTGG GCCGAACGCC 3051 TCGATCATCA GCCTGAAAAG TGACAAGAAA AGGAAGTTAG AGGCCAAACA 3101 AGAACCCAAA CAGAGCAAGA AGTTGAAGAA CAGAGAGACA AAGAACAAAA 3151 AAGATATGAA ACTGAAGCGG AAGAAATAGT GAAGAGAAAC TCGGGCATCT 3201 GTGTTTGATC ATGGGAAGAT ACTCTCACTA ACTGAACCCT CTCTGGCTGG 3251 ACTGTTAAAA GCAACGAGAG GCCCCGGCAC ACCTGGAAGC TGGCCGCGAA 3301 TTCGGCCTCT GGGCCTGTGT GTCTGTGAGC TCAACCTGGC TAAAGGCAGA 3351 GTCACTCCCA AATGGGTCTC TTTAGAACTT GATGGCTGGG CACTGCCATC 3401 TCTAGAATTG CCACGAGTCT CTCTCTTCCT GCCCAGTCCA GGGCCCTCCT 3451 TTCCTATAAG TTCATATTTT GCTTTGAGCC AGCTTTTTAG TCTCATTCCC 3501 ACACATGTGG AAGCCACGTT GCCTCTCGAC CGCCTGAGGC CCTTAAGTAC 3551 ATCGCTTTCT GGTGGTGCCC AGGAGGCTGC TGCTGGGCCG CTGGGTCTCT 3601 CTTTGTGGAC TTGTACCTGG AGCAGGAGGA ACTCCAGTCC GTCCCGGCAT

3651 CCATGGCAGC CCGCGGTTAG GTGCGCCAGG GTTTGCTGAT GTTGTCTTGT 3701 GCTGTTCCAC TCTTGGCTCC AGCAGACCCA CTGTCCCAGA AAAGCCTGAT 3751 CCTGTAGTTT ATGTAGAATG CCACATCTGC GTCCTCAAGA CCTGTTTCAT 3801 CCATTTGGGA AAAGATGTTG GGAAAGGCCA CTTTGCTCGC AGGGGTGAGG 3851 GGAAGGATAG AGAATCTATT TTTAATAAAT AACATTCTAG AATGAAAAAA 3901 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 3951 AAAAAAAAAA AAAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 102 bp to 3176 bp; peptide length: 1025 Category: similarity to unknown protein Classification: unclassified Prosite motifs: RGD (966-969) ATP GTP A (284-292)

1 MHRKKVDNRI RILIENGVAE RQRSLFVVVG DRGKDQVVIL HHMLSKATVK 51 ARPSVLWCYK KELGFSSHRK KRMRQLQKKI KNGTLNIKQD DPFELFIAAT 101 NIRYCYYNET HKILGNTFGM CVLQDFEALT PNLLARTVET VEGGGLVVIL 151 LRTMNSLKQL YTVTMDVHSR YRTEAHQDVV GRFNERFILS LASCKKCLVI 201 DDQLNILPIS SHVATMEALP PQTPDESLGP SDLELRELKE SLQDTQPVGV 251 LVDCCKTLDQ AKAVLKFIEG ISEKTLRSTV ALTAARGRGK SAALGLAIAG 301 AVAFGYSNIF VTSPSPDNLH TLFEFVFKGF DALQYQEHLD YEIIQSLNPE 351 FNKAVIRVNV FREHRQTIQY IHPADAVKLG QAELVVIDEA AAIPLPLVKS 401 LLGPYLVFMA STINGYEGTG RSLSLKLIQQ LRQQSAQSQV STTAENKTTT 451 TARLASARTL HEVSLQESIR YAPGDAVEKW LNDLLCLDCL NITRIVSGCP 501 LPEACELYYV NRDTLFCYHK ASEVFLQRLM ALYVASHYKN SPNDLQMLSD 551 APAHHLFCLL PPVPPTQNAL PEVLAVIQVC LEGEISRQSI LNSLSRGKKA 601 SGDLIPWTVS EQFQDPDFGG LSGGRVVRIA VHPDYQGMGY GSRALQLLQM 651 YYEGRFPCLE EKVLETPQEI HTVSSEAVSL LEEVITPRKD LPPLLLKLNE 701 RPAERLDYLG VSYGLTPRLL KFWKRAGFVP VYLRQTPNDL TGEHSCIMLK 751 TLTDEDEADQ GGWLAAFWKD FRRRFLALLS YQFSTFSPSL ALNIIQNRNM 801 GKPAQPALSR EELEALFLPY DLKRLEMYSR NMVDYHLIMD MIPAISRIYF 851 LNQLGDLALS AAQSALLLGI GLQHKSVDQL EKEIELPSGQ LMGLFNRIIR 901 KVVKLFNEVQ EKAIEEQMVA AKDVVMEPTM KTLSDDLDEA AKEFQEKHKK 951 EVGKLKSMDL SEYIIRGDDE EWNEVLNKAG PNASIISLKS DKKRKLEAKQ 1001 EPKQSKKLKN RETKNKKDMK LKRKK

BLASTP hits

No BLASTP hits available Alert BLASTP hits for DKFZphtes3_6cll, frame 3

TREMBL:CEAF3130_4 gene: "F55A12.8"; Caenorhabditis elegans cosmid F55A12., N = 1, Score = 2782, P = l.le-289

PIR:S55151 probable membrane protein YNL132w - yeast (Saccharomyces cerevisiae), N = 2, Score = 2549, P = 3.5e-273

SWISSPROT :YXX1_ACHAM HYPOTHETICAL PROTEIN (FRAGMENT)., N = 1, Score = 1013, P = 3.2e-102

SWISSPRO :YDK9_SCHPO HYPOTHETICAL 116.5 KD PROTEIN C20G8.09C IN CHROMOSOME I., N = 1, Score = 2843, P = 3.8e-296

>SWISΞPROT:YDK9_SCHPO HYPOTHETICAL 116.5 KD PROTEIN C20G8.09C IN CHROMOSOME I.

Length = 1,033

HSPs:

Score = 2843 (426.6 bits), Expect = 3.8e-296, P = 3.8e-296 Identities = 576/1033 (55%), Positives = 750/1033 (72%)

Query: 1 MHRKKVDNRIRILIENGVAERQRSLFVVVGDRGKDQVVILHHMLSKATVKARPSVLWCYK 60

M +K +D+RI LI+NG -E+QRS FVVVGDR +DQVV LH +LS++ V ARP+VLW YK Sbjct: 1 MPKKALDSRIPTLIKNGCQEKQRSFFVVVGDRARDQVVNLHWLLSQSKVAARPNVLWMYK 60

Query: 61 KEL-GFSSHRKKRMRQLQKKIKNGTLNIKQDDPFELFIAATNIRYCYYNETHKILGNTFG 119

K+L GF+SHRKKR +++K+IK G + +DPFELF + TNIRYCYY E+ KILG T+G Sbjct: 61 KDLLGFTSHRKKRENKIKKEIKRGIRDPNSEDPFELFCSITNIRYCYYKESEKILGQTYG 120

Query: 120 MCVLQDFEALTPNLLARTVETVEGGGLVVILLRTMNSLKQLYTVTMDVHSRYRTEAHQDV 179

M VLQDFEALTPNLLART+ETVEGGG+VV+LL +NSLKQLYT++MD+HSRYRTEAH DV Sbjct: 121 MLVLQDFEALTPNLLARTIETVEGGGIVVLLLHKLNSLKQLYTMSMDIHSRYRTEAHSDV 180

Query: 180 VGRFNERFILSLASCKKCLVIDDQLNILPISSHVATMEALPPQTPDESLGPSDLELRELK 239

RFNERFILSL +C+ CLVIDD+LN+LPIS ++ALPP +++ + ++EL+ Sbjct: 181 TARFNERFILSLGNCENCLVIDDELNVLPISGG-KNVKALPPTLEEDN—STQNSIKELQ 237

Query: 240 ESLQDTQPVGVLVDCCKTLDQAKAVLKFIEGISEKTLRSTVALTAARGRGKSAALGLAIA 299

ESL + P G LV KTLDQA+AVL F+E I EK+L+ TV+LTA RGRGKSAALGLAIA Sbjct: 238 ESLGEDHPAGALVGVTKTLDQARAVLTFVESIVEKSLKGTVSLTAGRGRGKSAALGLAIA 297

Query: 300 GAVAFGYSNIFVTSPSPDNLHTLFEFVFKGFDALQYQEHLDYEIIQSLNPEFNKAVIRVN 359

A+A GYSNIF+TSPSP+NL TLFEF+FKGFDAL Y+EH+DY+IIQS NP ++ A++RVN Sbjct: 298 AAIAHGYSNIFITSPSPENLKTLFEFIFKGFDALNYEEHVDYDIIQSTNPAYHNAIVRVN 357

Query: 360 VFREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKSLLGPYLVFMASTINGYEGT 419

+FR+HRQTIQYI P D+ LGQAELVVIDEAAAIPLPLV+ L+GPYLVFMASTINGYEGT Sbjct: 358 IFRDHRQTIQYISPEDSNVLGQAELVVIDEAAAIPLPLVRKLIGPYLVFMASTINGYEGT 417

Query: 420 GRSLSLKLIQQLRQQSAQSQVSTTAENKTTTTARLASARTLHEVSLQESIRYAPGDAVEK 479

GRSLSLKL+QQLR+QS S + NK+ + + + S RTL E+SL E IRYA GD +E Sbjct: 418 GRSLSLKLLQQLREQSRI—YSGSGNNKSDSQSHI-SGRTLKEISLDEPIRYAMGDRIEL 474

Query: 480 WLNDLLCLDCLN-ITRIVS-GCPLPEACELYYVNRDTLFCYHKASEVFLQRLMALYVASH 537

WLN LLCLD + ++R+ + G P P C LY V+RDTLF YH SE FLQR+M+LYVASH Sbjct: 475 WLNKLLCLDAASYVSRMATQGFPHPSECSLYRVSRDTLFSYHPISEAFLQRMMSLYVASH 534

Query: 538 YKNSPNDLQMLSDAPAHHLFCLLPPVPPTQNALPEVLAVIQVCLEGEISRQSILNSLSRG 597

YKNSPNDLQ++SDAPAH LF LLPPV LP+ + VIQ+ LEG ISR+SI+NSLSRG Sbjct: 535 YKNSPNDLQLMSDAPAHQLFVLLPPVDLKNPKLPDPICVIQLALEGSISRESIMNSLSRG 594

Query: 598 KKASGDLIPWTVSEQFQDPDFGGLSGGRVVRIAVHPDYQGMGYGSRALQLLQMYYEGRFP 657

++A GDLIPW +S+QFQD +F L G R+VRIAV P++ MGYG+RA+QLL Y+EG+F Sbjct: 595 QRAGGDLIPWLISQQFQDENFAALGGARIVRIAVSPEHVKMGYGTRAMQLLHEYFEGKFI 654

Query: 658 CLEEKVLETPQEIHTVSSEAV SLLEEVITPR—KDLPPLLLKLNERPAERLDYLGVS 712

E+ + + E + +L E I R K +PPLLLKL+E E L Y+GVS Sbjct: 655 SASEEFKAVKHSLKRIGDEEIENTALQTEKIHVRDAKTMPPLLLKLSELQPEPLHYVGVS 714

Query: 713 YGLTPRLLKFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKDFR 772

YGLTP L KFWKR G+ P+YLRQT NDLTGEH+C+ML+ L D WL AF ++F Sbjct: 715 YGLTPSLQKFWKREGYCPLYLRQTANDLTGEHTCVMLRVLEGRDSE WLGAFAQNFY 770

Query: 773 RRFLALLSYQFSTFSPSLALNIIQNRNMGKP AQPALSREELEALFLPYDLKRLEMY 828

RRFL+LL YQF F+ AL+++ N G + L+ EE+ +F YDLKRLE Y Sbjct: 771 RRFLSLLGYQFREFAAITALSVLDACNNGTKYVVNSTΞKLTNEEINNVFESYDLKRLESY 830 Query: 829 SRNMVDYHLIMDMIPAISRIYFLNQLGD-LALSAAQSALLLGIGLQHKSVDQLEKEIELP 887

S N++DYH+I+D++P ++ +YF + D + LS Q ++LL +GLQ+K++D LEKE LP Sbjct: 831 SNNLLDYHVIVDLLPKLAHLYFSGKFPDSVKLSPVQQSVLLALGLQYKTIDTLEKEFNLP 890

Query: 888 SGQLMGLFNRIIRKVVKLFNEVQEKAIEEQMVAAKDVVME PTMKTLSDDLDE 939

S QL+ + ++ +K++K +E++ K IEE++ + K P ++L ++L E

Sbjct: 891 SNQLLAMLVKLSKKIMKCIDEIETKDIEEELGSNKKTESSNSKLPEFTPLQQSLEEELQE 950

Query: 940 AAKEFQ-EKHKKEVGKLKSMDLSEYIIRGDDEEWNEVLNKAGPNASIISLKSDKKRKLEA 998

A E +K+ + ++DL +Y IRG++E+W KA N I R + Sbjct: 951 GADEAMLALREKQRELINAIDLEKYAIRGNEEDW KAAEN-QIQKTNGKGARVVSI 1004

Query: 999 KQEPKQSKKL--KNRETKNKKDMKLKRKK 1025

K E +++ L +++TK K K K +K Sbjct: 1005 KGEKRKNNSLDASDKKTKEKPSSKKKFRK 1033

Pedant information for DKFZphtes3_6cll, frame 3

Report for DKFZphtes3_6cll .3

[LENGTH] 1025

[MW] 115704.57

[pi] 8.50

[HOMOL] PIR:S55151 probable membrane protein YNL132w - yeast (Saccharomyces cerevisiae)

0.0

[FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YNL132w] 0.0

[FUNCAT] r general function prediction [H. influenzae, HI1254] 2e-05

[PROSITE] ATP_GTP_A 1

[PROSITE] RGD 1

[KW] Alpha_Beta

[KW] LOW_COMPLEXITY 11.80 %

SEQ MHRKKVDNRIRILIENGVAERQRSLFVVVGDRGKDQVVILHHMLSKATVKARPSVLWCYK SEG

PRD cccccccchhhhhhcccccccceeeeeeeeccccceeeeehhhhhhhhhhccceeehhhh

SEQ KELGFSSHRKKRMRQLQKKIKNGTLNIKQDDPFELFIAATNIRYCYYNETHKILGNTFGM SEG

PRD hhhcccchhhhhhhhhhhhhhhhcccccccccceeeecccceeeeeccccceeeccccee

SEQ CVLQDFEALTPNLLARTVETVEGGGLVVILLRTMNSLKQLYTVTMDVHSRYRTEAHQDVV SEG xxxxxxxxxxxxxxx

PRD eehhhhhccccchhhhhhhhhcccceeeeeeccchhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ GRFNERFILSLASCKKCLVIDDQLNILPISSHVATMEALPPQTPDESLGPSDLELRELKE SEG

PRD hhhhhhhhhhhcccceeeeeecceeeecccccccccccccccccccccccchhhhhhhhh

SEQ SLQDTQPVGVLVDCCKTLDQAKAVLKFIEGISEKTLRSTVALTAARGRGKSAALGLAIAG

SEG xxxxxxxxx

PRD hhcccccceeeeehhhhhhhhhhhhhhhhhhhhhhhhhhheeeccccccchhhhhhhhhh

SEQ AVAFGYSNIFVTSPSPDNLHTLFEFVFKGFDALQYQEHLDYEIIQSLNPEFNKAVIRVNV SEG xxx

PRD hhhhcccceeecccccccchhhhhhhhhhhhhhhhhhhhhheeeeeccccccceeeeeeh

SEQ FREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKSLLGPYLVFMASTINGYEGTG SEG

PRD hhhhhhheeeeccccccccccceeeehhhhhccchhhhhhhccceeeeeeeccccccccc

SEQ RSLSLKLIQQLRQQSAQSQVSTTAENKTTTTARLASARTLHEVSLQESIRYAPGDAVEKW SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD cchhhhhhhhhhhhhhhhhhhhccccccccccchhhhhhhhhhhhhhceeeccccchhhh

SEQ LNDLLCLDCLNITRIVSGCPLPEACELYYVNRDTLFCYHKASEVFLQRLMALYVASHYKN SEG xxxxxxxxxxx

PRD hhhhhhcccccceeeccccccccceeeeeeeccccccccchhhhhhhhhhhhhhhhhccc

SEQ SPNDLQMLSDAPAHHLFCLLPPVPPTQNALPEVLAVIQVCLEGEISRQSILNSLSRGKKA SEG

PRD cccccccccccccceeeeeeccccccccccchhhhhhhhhhccccchhhhhhhhcccccc

SEQ SGDLIPWTVSEQFQDPDFGGLSGGRVVRIAVHPDYQGMGYGSRALQLLQMYYEGRFPCLE SEG

PRD cccchhhhhhhhhhhccccccccceeeeeeccccccccccchhhhhhhhhhhhcccchhh

SEQ EKVLETPQEIHTVSSEAVSLLEEVITPRKDLPPLLLKLNERPAERLDYLGVΞYGLTPRLL SEG xxxxxxxxxx

PRD hhhhhccccccchhhhhhhhhhhhhhccccccccccccccccccceeeeccccccchhhh

SEQ KFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKDFRRRFLALLS

SEG

PRD hhhhhcccceeeeeccccccccceeeeeeecccccccccchhhhhhhhhhhhhhhhhhhh

SEQ YQFSTFSPSLALNIIQNRNMGKPAQPALSREELEALFLPYDLKRLEMYSRNMVDYHLIMD

SEG

PRD hhhhcchhhhhhhhhhhcccccccchhhhhhhhhhhhccchhhhhhhhhccchhhhhhhh

SEQ MIPAISRIYFLNQLGDLALSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIR

SEG xxxxxxxxxxxxxxxxxxxxx

PRD hhhhhhhhhhhhcccchhhhhhhhhhhhhhcchhhhhhhhhhhhhccccchhhhhhhhhh

SEQ KVVKLFNEVQEKAIEEQMVAAKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKSMDL

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhcc

SEQ SEYIIRGDDEEWNEVLNKAGPNASIISLKSDKKRKLEAKQEPKQSKKLKNRETKNKKDMK

SEG xxxxxxxxxxxxxxx

PRD cceeecccchhhhhhhhhccccceeeeeeccchhhhhhhhcccccccccccccccchhhh

SEQ LKRKK

SEG xxxxx

PRD hhccc

Prosite for DKFZphtes3_6cll .3

PS00016 966->969 RGD PDOC00016

PS00017 284->292 ATP GTP A PDOC00017

(No Pfam data available for DKFZphtes3_6cll .3)

DKFZphtes3_6dl6

group: testes derived

DKFZphtes3_6dl6 encodes a novel 695 ammo acid protein nearly identical to a sequence from human PAC clone WUGSC:H_DJ1185I07.2.

The cDNA is different to the proposed gene model: it contains additional exons. No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes .

WUGSC:H_DJ1185I07.2, differences to genmodel differences to genmodel of WUGSC:H_DJ1185I07.2 two exons skippt,

Sequenced by BMFZ

Locus: /map="7qll .23-q21"

Insert length: 4572 bp

Poly A stretch at pos. 4540, polyadenylation signal at pos. 4520

1 GGCGGCGCTA GCTTCGGAGT CTCCCGCGCG CACCTCAGCC GCCTCCTAGC 51 GGCGCGGCGC TCGCTCCTAC GCCTAAAATG ACCAATGTGT GATTTCAGTG

101 GAATAAATGG CGTCCAAAGT CACAGATGCT ATAGTCTGGT ATCAAAAGAA

151 GATTGGAGCA TATGATCAAC AAATATGGGA AAAATCTGTT GAACAGAGAG

201 AAATCAAGGG GCTAAGGAAT AAACCAAAGA AAACAGCACA TGTGAAACCA

251 GACCTCATAG ATGTTGATCT TGTAAGAGGG TCTGCATTTG CAAAGGCAAA

301 GCCTGAAAGT CCTTGGACTT CTCTGACCAG AAAGGGAATT GTTCGAGTTG

351 TATTTTTCCC CTTTTTCTTC CGGTGGTGGT TACAAGTAAC ATCAAAGGTC

401 ATCTTTTTCT GGCTTCTTGT CCTTTATCTT CTTCAAGTTG CTGCAATAGT

451 ATTATTCTGC TCCACTTCTA GCCCACACAG CATACCTCTG ACAGAGGTGA

501 TTGGGCCGAT ATGGCTGATG CTGCTCCTGG GAACTGTGCA TTGCCAGATT

551 GTTTCCACAA GAACACCCAA ACCTCCTCTA AGTACAGGGG GTAAAAGAAG

601 AAGGAAATTA AGAAAAGCAG CCCATTTGGA AGTACATAGG GAAGGAGATG

651 GTTCTAGTAC CACAGATAAC ACACAAGAGG GAGCAGTTCA GAACCACGGT

701 ACAAGCACCT CTCACAGCGT TGGCACTGTC TTCAGAGATC TCTGGCATGC

751 TGCTTTCTTT TTATCAGGAT CAAAGAAAGC AAAGAATTCA ATTGATAAAT

801 CAACTGAAAC TGACAATGGC TATGTATCCC TTGATGGGAA GAAGACTGTT

851 AAAAGCGGTG AAGATGGAAT ACAAAACCAT GAACCTCAGT GTGAAACTAT

901 TCGACCAGAA GAGACAGCCT GGAACACAGG AACACTGAGG AATGGTCCTA

951 GCAAAGATAC CCAAAGGACA ATAACAAATG TCTCTGATGA AGTCTCCAGT 1001 GAGGAAGGTC CTGAAACAGG ATACTCATTA CGTCGTCATG TGGACAGGAC 1051 TTCTGAAGGT GTTCTTCGGA ATAGAAAGTC ACACCATTAT AAGAAACATT 1101 ACCCTAATGA GGACGCCCCT AAATCGGGTA CTAGTTGCAG CTCTCGCTGT 1151 TCAAGTTCCA GACAGGATTC TGAGAGTGCA AGGCCAGAAT CTGAAACAGA 1201 AGATGTGTTA TGGGAAGACT TGTTACATTG TGCAGAATGC CATTCATCTT 1251 GTACCAGTGA GACAGATGTG GAAAATCATC AGATTAATCC ATGTGTGAAA 1301 AAAGAATATA GAGATGACCC TTTTCATCAG AGTCATTTGC CCTGGCTCCA 1351 TAGTTCCCAC CCAGGATTAG AAAAAATAAG TGCTATAGTA TGGGAAGGTA 1401 ATGATTGTAA GAAAGCAGAC ATGTCTGTAC TTGAAATCAG TGGAATGATA 1451 ATGAACAGAG TGAACAGCCA TATACCAGGA ATAGGATACC AGATTTTTGG 1501 AAATGCAGTC TCTCTCATAC TGGGTTTAAC TCCATTTGTT TTCCGACTTT 1551 CTCAAGCTAC AGACTTGGAA CAACTCACAG CACATTCTGC TTCAGAACTT 1601 TATGTGATTG CATTTGGTTC TAATGAAGAT GTCATAGTTC TTTCTATGGT 1651 TATAATAAGT TTTGTGGTTC GCGTGTCTCT TGTGTGGATT TTCTTTTTTT 1701 TGCTCTGTGT AGCAGAAAGA ACTTATAAAC AGCGATTACT TTTTGCAAAA 1751 CTCTTTGGAC ATTTAACATC TGCAAGGAGG GCTCGAAAAT CTGAGGTTCC 1801 TCATTTCCGG TTGAAGAAAG TACAGAATAT AAAAATGTGG CTATCTCTCC 1851 GTTCCTATCT TAAGCGTCGA GGTCCTCAGC GATCAGTTGA TGTAATAGTT 1901 TCATCTGCTT TCTTATTGAC TATCTCAGTT GTATTTATCT GTTGTGCCCA 1951 GATAAACCTC TACTTGAAAA TGGAGAAAAA ACCTAACAAA AAGGAGGAAC 2001 TGACACTAGT GAATAATGTT TTAAAACTGG CTACTAAACT GCTAAAGGAG 2051 TTGGACAGTC CTTTTAGATT ATATGGGCTT ACAATGAATC CGCTGCTTTA 2101 TAACATCACC CAGGTTGTTA TCCTGTCAGC TGTTTCTGGT GTTATCAGTG 2151 ACTTGCTTGG ATTTAATTTA AAGCTATGGA AGATTAAGTC ATGACAATTC 2201 AAAGAAAAGA AGATGTAGCC TCTTTTCCAG AATAAGAGTA CTGACTAAGC 2251 TGCCTGAAAG CTTGTCACTG ATTCTTTGCT TCAGGAGTCT CAGCTAGGGA 2301 GTTGAAGTGT TTACATCAGA CTGTCTTGTG CAATTCTTAT ATTTATTTTA 2351 CTGGTTCACT TTTTTTTACA TTTATTTTAG TCTTTATATT TTTATTTTTA 2401 AGCATTGATG TACTTAGTTG TTGAAAGGGT GATGAAACTG ATATCCAGAT 2451 ACTTGAGATC CTGGTAATTG GTCATAAATA ATTGGCAAAA TAACAAATTG 2501 TGAAAATAGA AGCCATTGCT CAGCACCGTT TCTCCATCAA TGCCGTGAAC 2551 TTGCCTTACT TGAGGAAAAA TTCTTTAACT TTGGAATATT GCATTGAACT 2601 CAGCTATACA CATAAAACAT TTTCTTTGGT AAATCAAGAT CCAGTCAGGG 2651 TTTCTCTTGA ATTATTTTGG AACAATGCCA GGATCCAAAC TGATTAAGTT 2701 ACAGTTTAAG CACCCTTCAG TATTAATATA TACGGTATTA TATAACAGGT 2751 CAACAAGTGC TCTTTGATGA TAAAACTTGT AATAGAGCAA TAATTGTAAA

2801 TGGTTACCAT ACTGTAAGAT ATTTTGATAA AAATTAACTA GTAATACTTG

2851 TATTTATTTG AAACACTGGG CTGTTTGCAC AGCTCCAACT GTGCATGCTC

2901 AAAATGTGCA CTTTTTAAAA TTGTTACTTT TAATGCGTAT CTTTATATGG

2951 GATCTGTTAT AGTATACTAG GGCATGATAT GGTATCCTTT TGAGTGAGGT

3001 ATATACTCAT CTCACAAGTG AAGTGCCTAC TGATATTACT AAAGTACATT

3051 ATGTTTACTC AAGTAAATAA TTTTCTCCCC ATGGTACACT CTAGTGTAGG

3101 CTATTCATAC CACACTGAAA TGAACAACTG AAGAATAAGG CTAAGAACCA

3151 ATAAAATATT TCTCTAATTG CTAGTTGTAA AACTGTATCC AAATTTTCAG

3201 AAAAGACAGC TTCAGCTTGC AAATTCTATC CTCTAAACTT ATCTGGTGCA

3251 TTCTCCCCAC CCCACCCCCA TTATATAAGG GCTATTTTAG ATGCTTTTAA

3301 CCTCCCCAAC AAATAATTTG CCAAGTGTCC AATGAGAACT TATCATGTTG 3351 GTGTGTTAGG TAAATCGGGC AAATATGATA GTGTCTTACA TTGGGCCTTG

3401 ATTTTAAGTT GTTATATTTG TACAATCGAG TATTTTAGAA ATTACATGAA

3451 ACATGAAACA GTTTTTGCAA TTTTTTTTAA ACTGGGCATC TGGTTTCTAA

3501 AAATTTATTT GAAACAATCT AGAATTTTCT TGGTGCAAAG TGTATCATGT 3551 GGAATATCCT CATATTTTTA CCATATTTTA AGAACTTTAA GACGATTAAT

3601 TGTAAATAAT TTATTTGATT GGTGCAGTTC TAATCCCTAA ATCATAATCT

3651 TAAAATCAGG AATGTGTGGA GAACAGAGCC ATGTCATATC ACTTTGCTCT

3701 TACCATTCCT TTTGATCAGC CTCAATTCAG CCTCATTGTG TAGTATGTTT

3751 TTTCTTTCTA TGAAAAACAA CAGAAAGCAT TTCATTTTAT TTGCCTATGT

3801 TCAAATATGT TTAATAATGA CCAAAGTGCA TTCTGAGTTT TTTCAAGGAA

3851 TGTAATACTG GAGCTTTAAG AACATACTTA GTTTCTCATG TGAAAACTTA

3901 GGCTTTGTCT GATGTTTTTC CTTCCTCTAT TGTCTAATGT TGAGGTTGTT

3951 TTTAGGAATT ATGTTTTATA AACTTTTTCA ATATAAGGTA CATGCCTATA

4001 CAGAACTTAA CATTTTGCAC AGAATATATC AAATATATTT TGAGAAAAAA

4051 AGTACGGCAT GAGTTCTGTT AGGAATAAAA GATGAAACTA TTGTATCTCA

4101 CAAAAAATCT TATTTCAGAA TGGAAATATT TTTGAGAAAA GTAGCTGAGT

4151 ATACTGGTTT AAGAAAATGC TTGTTTTAGA TTGAGGTTAA CTTAGAGTTG

4201 GGAGTTGATT TATTAAGTAC AGTATACCTC TCAACAGTTT ATAAATAATA

4251 TGTTGAATTA TGTCAGTGTG GGCAGCAGTA GAATACTAAA AGGAAAATGT

4301 CATGTTAAGC AATTTCAGAA CATTAACTGA ACTATTTTCA AAGCAGAAAA

4351 ATTGACATTG CTGCCTTTAA GAATACCATG AATGTAAGAA ATTGAAAGAA

4401 ATTGTAAAAT ATCACATAAT ATAGAAATGG CAGTTCAAAG AGAATTGTGG

4451 CAGATGTTGT GTGTGAACTG TTGTTTCTTT GCCACATGTG TTGTATTTGA

4501 AAGTTTTACA GTAAGTTTAA AATAAAACAT TCTGTGACTG AAAAAAAAAA 4551 AAAAAAAAAA AAAAAAAAAA AA

BLAST Results

No BLAST result

Medline entries

No Medlme entry

Peptide information for frame 2

ORF from 107 bp to 2191 bp; peptide length: 695

Category: known protein

Classification: unclassified

Prosite motifs: CYTOCHROME C (375-381)

1 MASKVTDAIV WYQKKIGAYD QQIWEKSVEQ REIKGLRNKP KKTAHVKPDL

51 IDVDLVRGSA FAKAKPESPW TSLTRKGIVR VVFFPFFFRW WLQVTSKVIF

101 FWLLVLYLLQ VAAIVLFCST SSPHSIPLTE VIGPIWLMLL LGTVHCQIVS

151 TRTPKPPLST GGKRRRKLRK AAHLEVHREG DGSSTTDNTQ EGAVQNHGTS

201 TSHSVGTVFR DLWHAAFFLS GSKKAKNSID KSTETDNGYV SLDGKKTVKS

251 GEDGIQNHEP QCETIRPEET AWNTGTLRNG PSKDTQRTIT NVSDEVSSEE

301 GPETGYSLRR HVDRTSEGVL RNRKSHHYKK HYPNEDAPKS GTSCSSRCSS

351 SRQDSESARP ESETEDVLWE DLLHCAECHS SCTSETDVEN HQINPCVKKE

401 YRDDPFHQSH LPWLHSSHPG LEKISAIVWE GNDCKKADMS VLEISGMIMN

451 RVNSHIPGIG YQIFGNAVSL ILGLTPFVFR LSQATDLEQL TAHSASELYV

501 IAFGSNEDVI VLSMVIISFV VRVSLVWIFF FLLCVAERTY KQRLLFAKLF

551 GHLTSARRAR KSEVPHFRLK KVQNIKMWLS LRΞYLKRRGP QRSVDVIVSS

601 AFLLTISVVF ICCAQINLYL KMEKKPNKKE ELTLVNNVLK LATKLLKELD

651 SPFRLYGLTM NPLLYNITQV VILSAVSGVI SDLLGFNLKL WKIKS

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_6dl6, frame 2

PIR:S38170 SRP40 protein - yeast (Saccharomyces cerevisiae), N = 1, Score = 100, P = 0.08

TREMBL :AC004990_1 gene: "WUGSC:H_DJ1185I07.2"; Homo sapiens PAC clone DJ1185I07 from 7qll.23-q21, complete sequence., N = 2, Score = 2693, P = 0

>TREMBL:AC004990_1 gene: "WUGSC:H_DJ1185I07.2"; Homo sapiens PAC clone DJ1185I07 from 7qll.23-q21, complete sequence. Length = 588

HSPs:

Score = 2693 (404.1 bits), Expect = O.Oe+00, Sum P(2) = O.Oe+00 Identities = 510/515 (99%), Positives = 512/515 (99%)

Query: 35 GLRNKPKKTAHVKPDLIDVDLVRGSAFAKAKPESPWTSLTRKGIVRVVFFPFFFRWWLQV 94

GLRNKPKKTAHVKPDLIDVDLVRGSAFAKAKPESPWTSLTRKGIVRVVFFPFFFRWWLQV Sbjct: 1 GLRNKPKKTAHVKPDLIDVDLVRGSAFAKAKPESPWTSLTRKGIVRVVFFPFFFRWWLQV 60

Query: 95 TSKVIFFWLLVLYLLQVAAIVLFCSTSSPHSIPLTEVIGPIWLMLLLGTVHCQIVSTRTP 154

TSKVIFFWLLVLYLLQVAAIVLFCSTSSPHSIPLTEVIGPIWLMLLLGTVHCQIVΞTRTP Sbjct: 61 TSKVIFFWLLVLYLLQVAAIVLFCSTSSPHSIPLTEVIGPIWLMLLLGTVHCQIVSTRTP 120

Query: 155 KPPLSTGGKRRRKLRKAAHLEVHREGDGSSTTDNTQEGAVQNHGTSTSHSVGTVFRDLWH 214

KPPLSTGGKRRRKLRKAAHLEVHREGDGSSTTDNTQEGAVQNHGTSTSHSVGTVFRDLWH Sbjct: 121 KPPLSTGGKRRRKLRKAAHLEVHREGDGSΞTTDNTQEGAVQNHGTSTSHSVGTVFRDLWH 180

Query: 215 AAFFLSGSKKAKNSIDKSTETDNGYVSLDGKKTVKSGEDGIQNHEPQCETIRPEETAWNT 274

AAFFLSGSKKAKNSIDKSTETDNGYVSLDGKKTVKSGEDGIQNHEPQCETIRPEETAWNT Sbjct: 181 AAFFLSGSKKAKNSIDKSTETDNGYVSLDGKKTVKSGEDGIQNHEPQCETIRPEETAWNT 240

Query: 275 GTLRNGPSKDTQRTITNVSDEVSSEEGPETGYSLRRHVDRTSEGVLRNRKSHHYKKHYPN 334

GTLRNGPSKDTQRTITNVSDEVSSEEGPETGYSLRRHVDRTSEGVLRNRKSHHYKKHYPN Sbjct: 241 GTLRNGPSKDTQRTITNVSDEVSSEEGPETGYSLRRHVDRTSEGVLRNRKSHHYKKHYPN 300

Query: 335 EDAPKSGTSCSSRCSSSRQDSESARPESETEDVLWEDLLHCAECHSSCTSETDVENHQIN 394

EDAPKSGTSCSSRCSSSRQDSESARPESETEDVLWEDLLHCAECHSSCTSETDVENHQIN Sbjct: 301 EDAPKSGTΞCSSRCSSSRQDSESARPESETEDVLWEDLLHCAECHSSCTΞETDVENHQIN 360

Query: 395 PCVKKEYRDDPFHQSHLPWLHSSHPGLEKISAIVWEGNDCKKADMSVLEISGMIMNRVNS 454

PCVKKEYRDDPFHQSHLPWLHSSHPGLEKISAIVWEGNDCKKADMSVLEISGMIMNRVNS Sbjct: 361 PCVKKEYRDDPFHQSHLPWLHSSHPGLEKISAIVWEGNDCKKADMSVLEISGMIMNRVNS 420

Query: 455 HIPGIGYQIFGNAVSLILGLTPFVFRLSQATDLEQLTAHSASELYVIAFGSNEDVIVLSM 514

HIPGIGYQIFGNAVSLILGLTPFVFRLSQATDLEQLTAHSASELYVIAFGSNEDVIVLSM Sbjct: 421 HIPGIGYQIFGNAVSLILGLTPFVFRLSQATDLEQLTAHSASELYVIAFGSNEDVIVLSM 480

Query: 515 VIISFVVRVSLVWIFFFLLCVAERTYKQRLLFAKL 549

VIISFVVRVSLVWIFFFLLCVAERTYKQ L+ K+ Sbjct: 481 VIISFVVRVΞLVWIFFFLLCVAERTYKQINLYLKM 515

Score = 409 (61.4 bits), Expect = O.Oe+00, Sum P(2) = O.Oe+00 Identities = 92/115 (80%), Positives = 98/115 (85%)

Query: 595 DVIVSS AFLLTISVVFI CCA QINLYLKMEKKPNKKEELTLVNNVLK 640

DVIV S +F++ +S+V+I C A QINLYLKMEKKPNKKEELTLVNNVLK Sbjct: 474 DVIVLSMVIISFVVRVSLVWIFFFLLCVAERTYKQINLYLKMEKKPNKKEELTLVNNVLK 533

Query: 641 LATKLLKELDSPFRLYGLTMNPLLYNITQVVILSAVSGVISDLLGFNLKLWKIKS 695

LATKLLKELDSPFRLYGLTMNPLLYNITQVVILSAVΞGVISDLLGFNLKLWKIKS Sbjct: 534 LATKLLKELDSPFRLYGLTMNPLLYNITQVVILSAVSGVISDLLGFNLKLWKIKS 588

Pedant information for DKFZphtes3_6dl6, frame 2

Report for DKFZphtes3_6dl6.2

[LENGTH] 695

[MW] 78466.68

[pi] 9.30

[HOMOL] TREMBL :AC004990_1 gene: "WUGSC :H_DJ1185I07.2"; Homo sapiens PAC clone DJ1185I07 from 7qll.23-q21, complete sequence. 0.0 [PROSITE] CYTOCHROME_C 1 [KW] TRANSMEMBRANE 6

[KW] LOW COMPLEXITY 5.32

SEQ MASKVTDAIVWYQKKIGAYDQQIWEKSVEQREIKGLRNKPKKTAHVKPDLIDVDLVRGSA

SEG

PRD ccceeeeehhhhhhhcccchhhhhhhhhhhhhhhcccccccccccccccceeeeeeccch

MEM

SEQ FAKAKPEΞPWTSLTRKGIVRVVFFPFFFRWWLQVTSKVIFFWLLVLYLLQVAAIVLFCST

SEG xxxxxxxxxxx

PRD hhhhcccccccccccccceeeeecchhhhhhhhhhhhhhhhhhhhhhhhhhhhhheeecc

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ SSPHSIPLTEVIGPIWLMLLLGTVHCQIVSTRTPKPPLSTGGKRRRKLRKAAHLEVHREG

SEG xxxxxxxx

PRD ccccccceeeeehhhhhhhhhhhhheeeeeeccccccccccchhhhhhhhhhhhheeecc

MEM MMMMMMMMMMMMMMMMMMMMMMM

SEQ DGSSTTDNTQEGAVQNHGTSTSHSVGTVFRDLWHAAFFLSGSKKAKNSIDKSTETDNGYV

SEG

PRD cccccccccceeeeeeccccccccchhhhhhhhhhhhhhcccchhhhhcccccccccccc

MEM

SEQ SLDGKKTVKSGEDGIQNHEPQCETIRPEETAWNTGTLRNGPSKDTQRTITNVSDEVΞSEE

SEG

PRD cccccceeecccccccccccccccccccceeeeccccccccccccceeeecccccccccc

MEM

SEQ GPETGYΞLRRHVDRTSEGVLRNRKSHHYKKHYPNEDAPKSGTSCSSRCSSSRQDSESARP

SEG xxxxxxxxxxxxxxxxxx ...

PRD ccccceeeeeeccccccchhhhhhcccccccccccccccccccccccccccccccccccc

MEM

SEQ ESETEDVLWEDLLHCAECHSSCTSETDVENHQINPCVKKEYRDDPFHQSHLPWLHSSHPG

SEG

PRD cccchhhhhhhhhhhhcccccccccccccccccccceeeeeccccccccccccccccccc

MEM

SEQ LEKISAIVWEGNDCKKADMSVLEISGMIMNRVNSHIPGIGYQIFGNAVSLILGLTPFVFR

SEG

PRD cccceeeeeecccccccceeeeehhhhhhhhhccccccccccccccccceeecccccchh

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ LSQATDLEQLTAHSASELYVIAFGSNEDVIVLSMVIISFVVRVSLVWIFFFLLCVAERTY

SEG

PRD hhhhhhhhhhhhcccceeeeeeeccccceeeehhhhhhhhcchhhhhhhhhhhhhhhhhh

MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM

SEQ KQRLLFAKLFGHLTSARRARKSEVPHFRLKKVQNIKMWLSLRSYLKRRGPQRSVDVIVSS

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhccccccceeeeeehhhhhhhhhhhhccccceeeeeeee

MEM MMMMMMM

SEQ AFLLTISVVFICCAQINLYLKMEKKPNKKEELTLVNNVLKLATKLLKELDSPFRLYGLTM

SEG

PRD eeeeeeeeeeeeeehhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhccccceeeeccc

MEM MMMMMMMMMMMMMMMMMMM

SEQ NPLLYNITQVVILSAVSGVISDLLGFNLKLWKIKS

SEG

PRD cchhhhheeeeeeeeecchhhhhccceeeeeeccc

MEM MMMMMMMMMMMMMMMMMMMMMMMMM

Prosite for DKFZphtes3_6dl6.2 PS00190 375->381 CYTOCHROME C PDOC00169

(No Pfam data available for DKFZphtes3_6dl6.2) DKFZphtes3_72kll

group: testes derived

DKFZphtes3_72kll encodes a novel 233 ammo acid protein with similarity to S. pombe hypothetical repeat-containing protein.

The novel protein contains 5 leucine zippers and a microbodies C-terrainal targeting signal (S- K-L) signature. This sequence is responsible for transport of proteins from free polysomes into the microbodies. No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . similarity to S. pombe hypothetical repeat-containing protein complete cDNA, complete eds, 6 EST hits (3 from testis derived librarys)

Sequenced by DKFZ

Locus : unknown

Insert length: 1134 bp

Poly A stretch at pos. 1124, polyadenylation signal at pos. 1088

1 AACCTTTCAA GTGCCCCCTC CTTTCCTTAA AGTCTTTTAT AGGGGTCCCC

51 TTCTTGGCCA TCTCCATCCT GTGAGTCAGG ACTGAAAGGG CACAGACAGG

101 TCACTGCCAG CATTGTTGGG GCAAGCCTGC AAGCACGCAT CACTGGGGAT

151 CTGACATGAC AATGGCCGCC TGCCCCCTCT GAGGGCTACA GGACTTACCC

201 CAGTGGGAAG CAGCTAAGCA GGTCTGACCA GCCGACCTGG ACCTGGCCAA

251 GGGTCCTGTC ATCCCTCATG GCCACCCCGC CATTCCGGCT GATAAGGAAG

301 ATGTTTTCCT TCAAGGTGAG CAGATGGATG GGGCTTGCCT GCTTCCGGTC

351 CCTGGCGGCA TCCTCTCCCA GTATTCGCCA GAAGAAACTA ATGCACAAGC

401 TGCAGGAGGA AAAGGCTTTT CGCGAAGAGA TGAAAATTTT TCGTGAAAAA

451 ATAGAGGACT TCAGGGAAGA GATGTGGACT TTCCGAGGCA AGATCCATGC

501 TTTCCGGGGC CAGATCCTGG GTTTTTGGGA AGAGGAGAGA CCTTTCTGGG

551 AAGAGGAGAA AACCTTCTGG AAAGAGGAAA AATCCTTCTG GGAAATGGAA

601 AAGTCTTTCA GGGAGGAAGA GAAAACTTTC TGGAAAAAGT ACCGCACTTT

651 CTGGAAGGAG GATAAGGCCT TCTGGAAAGA GGACAATGCC TTATGGGAAA

701 GAGACCGGAA CCTTCTTCAG GAGGACAAGG CCCTGTGGGA GGAAGAAAAG

751 GCCCTGTGGG TAGAGGAAAG AGCCCTCCTT GAGGGGGAGA AAGCCCTGTG

801 GGAAGATAAA ACGTCCCTCT GGGAGGAAGA GAATGCCCTC TGGGAGGAAG

851 AGAGGGCCTT CTGGATGGAG AACAATGGCC ACGTTGCCGG AGAGCAGATG

901 CTCGAAGATG GGCCCCACAA CGCCAACAGA GGGCAGCGCT TGCTGGCCTT

951 CTCCCGAGGC AGGGCGTAGC CAGCATGCAG GTGCAGGGCC CTGTGGTCCA

1001 GACTCCCCTG GGTTGGGATT CAAGTCCAGG GTGAGCCCAT GTGCTGGAGA

1051 AAATACACAC TCATTGGTCT CCTTGCTTTG AAAGATCCAA TAAAGTCCTG

1101 AGGCAAGGTT TGGAAAACCA ACTTAAAAAA AAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 1

ORF from 268 bp to 966 bp; peptide length: 233 Category: similarity to known protein Prosite motifs: MICROBODIES_CTER (231-234) LEUCINE_ZIPPER (142-164) LEUCINE_ZIPPER (149-171) LEUCINE_ZIPPER (156-178) LEUCINE_ZIPPER (163-185) LEUCINE_ZIPPER (170-192) LEUCINE ZIPPER (170-192) 1 MATPPFRLIR KMFSFKVSRW MGLACFRSLA ASSPSIRQKK LMHKLQEEKA

51 FREEMKIFRE KIEDFREEMW TFRGKIHAFR GQILGFWEEE RPFWEEEKTF

101 WKEEKSFWEM EKSFREEEKT FWKKYRTFWK EDKAFWKEDN ALWERDRNLL

151 QEDKALWEEE KALWVEERAL LEGEKALWED KTSLWEEENA LWEEERAFWM

201 ENNGHVAGEQ MLEDGPHNAN RGQRLLAFSR GRA

BLASTP hits

Entry SPCC330_4 from database TREMBLNEW: gene: "SPCC330.04c"; product: "hypothetical repeat-containing protein"

S. pombe chromosome III cosmid c330.

Score = 149, P = 1.6e-08, identities = 55/187, positives = 88/187

Entry A45973 from database PIR: trichohyalm - human

Score = 147, P = 3.0e-07, identities = 57/194, positives = 94/194

Alert BLASTP hits for DKFZphtes3_72kll, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphtes3_72kll, frame 1

Report for DKFZphtes3_72kll .1

[LENGTH] 233

[MW] 28752.65

[pi] 5.70

[PROSITE] LEUCINE_ZIPPER 5

[PROSITE] MICROBODIES_CTER

[PROSITE] MYRISTYL 1

[PROSITE] CK2_PHOSPHO_SITE

[PROSITE] PKC_PHOSPHO_SITE

[KW] All_Alpha

[KW] LOW COMPLEXITY 15.45

SEQ MATPPFRLIRKMFSFKVSRWMGLACFRSLAASSPSIRQKKLMHKLQEEKAFREEMKIFRE SEG PRD cccccchhhhhhhhhhhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhhhhhhh

SEQ KIEDFREEMWTFRGKIHAFRGQILGFWEEERPFWEEEKTFWKEEKSFWEMEKSFREEEKT SEG xxxxxxxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhcccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhh

SEQ FWKKYRTFWKEDKAFWKEDNALWERDRNLLQEDKALWEEEKALWVEERALLEGEKALWED SEG PRD hhhhcccccccccchhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ KTSLWEEENALWEEERAFWMENNGHVAGEQMLEDGPHNANRGQRLLAFSRGRA SEG ... xxxxxxxxxxxx

PRD ccchhhhhhhhhhhhhhhhhhccccchhhhhhcccccccccchhhhhhhhccc

Prosite for DKFZphtes3_72kll .1

PS00005 14->17 PKC_PHOSPHO_SITE PDOC00005 PS00005 35->38 PKC_PHOS PHO_S ITE PDOC00005 PS00005 71->74 PKC_PHOS PHO_S ITE PDOC00005 PS00005 113-M16 PKC_PHOSPHO_SITE PDOC00005 PS00006 106->110 CK2_PHOSPHO_SITE PDOC00006 PS00006 113->117 CK2_PHOSPHO_SITE PDOC00006 PS00006 183->187 CK2_PHOSPHO_SITE PDOC00006 PS00008 81->87 MYRISTYL PDOC00008 PS00342 231->234 MICROBODIES_CTER PDOC00299 PS00029 142->164 LEUCINE_ZIPPER PDOC00029 PS00029 149-M71 LEUCINE_ZIPPER PDOC00029 PS00029 156->178 LEUCINE_ZIPPER PDOC00029 PS00029 163->185 LEUCINE_ZIPPER PDOC00029 PS00029 170->192 LEUCINE ZIPPER PDOC00029

(No Pfam data available for DKFZphtes3 72kll.l) DKFZphtes3_72kl5

group: cell structure and motility

DKFZphtes3_72kl5 encodes a novel 188 amino acid protein with strong similarity to Rattus norvegicus actin-fllament binding protein Frabin.

FGDl-related F-actin-binding protein (Farbm/FGDl) is a novel F-actm-bmding protein. The gene locus fgdl seems to be responsible for faciogemtal dysplasia or Aarskog-Scott syndrome. Frabin binds F-actin and shows F-actm-cross-linking activity. Overexpression of frabin in Swiss 3T3 cells and COS7 cells induces cell shape change and c-Jun N-terminal kinase activation, as described for FGDl. Because FGDl has been shown to serve as a GDP/GTP exchange protein for Cdc42 small G protein, it is likely that frabin is a direct linker between Cdc42 and the actin cytoskeleton. Cdc42p is an esin yeast, Cdc42p transduces signals to the actin cytoskeleton to initiate and maintain polarized growth and to mitogen-activated protein morphogenesis. In mammalian cells, Cdc42p regulates a variety of actm-dependent events and induces the JNK/SAPK protein kinase cascade, which leads to the activation of transcription factors within the nucleus. The novel protein seems to be the human orthologue of rat frabin.

The new protein can find application in modulating of cell structure and motility as well as modulation of the JNK/SAPK pathway. strong similarity to act -filament binding protein Frabin

Sequenced by DKFZ

Locus : unknown

Insert length: 1845 bp

Poly A stretch at pos. 1835, polyadenylation signal at pos. 1816

1 GTGATGGAGA GTGCTGTTAT GATAGATGAA TCTAGGAAAG CCTCTTTGGA

51 GATGTGATAC CTGAACAGAA CCCCGAATGA TAAGAAGAAA TACCAGTGTT

101 TTAGGAGAGA TTGTCCTAAG CAGAGAACAG CAGCTGCAAA GACCCCAAGA

151 CACATACACT TGGTTATTAA GAATGGGAGC AGCAAGGAGT ATGGCAAGAA

201 CACAGTGAGT TTTCCCTTGA GTGTGTGAGG AAGCCCTCAG AGTTTGTGAC

251 TGACTTGTAG AGGTTCTAGT GGAGGGGATC AGAGTGGAAA CAAAGAGACC

301 AGTTAAAAAG GTATGGCAGC ATGAATAAAA AAGTTTTGAG AGTATTCATT

351 ATGCCTTCCA AATAAAAAAC TCTTTGGTTC ATAATTTGTT CATAAATTAA

401 GGACTGGCTA CACTGTACTA TTTAAAAATG TTAAGAAACA TCAATAAGTA

451 AAAATGTTAG GAAGAGATGA TAAATACGTA AGTATTATAT CTAACTAAGT

501 CTTTACTAAC TAGTCACATT ATTAAACAGT GCAAGGATCA AGAAAAGTTA

551 AGCGTTGAAA AATAAATAAA TAAGTTATAA ATAAAATAAA CAGCCCAAGG

601 AAATGTTCCA GTCCCCATAG GTAGACTCGG GGTCATCTTC TTTATTTAAA

651 TCTTTATTTA AATGTGGATA GCATCCCAAG AGACTTGGGT CTACACTAAG

701 AATATTCAAA TCCATGTTTC TGAAACCATC AGAGATAGAA AAAAAAAGTA

751 GCGAATATCC CTTTTCAACT GGAATAAACT TGTCTTAATT CTAGAACTTT

801 TCCATACCAA TGTTTTCATG CTTCCTTTGT ATTTTATCTT TTAGCTCATT

851 ATCAAATTAT AGTGATTTGA AGAAAGAGTC TGCTGTGAAC CTAAATGCTC

901 CTAGAACCCC AGGAAGGCAT GGATTGACAA CCACACCTCA ACAAAAACTC

951 CTCTCCCAGC ACTTGCCACA GAGGCAGGGA AATGATACAG ATAAGACTCA

1001 GGGTGCACAG ACTTGTGTGG CCAACGGTGT AATGGCAGCA CAAAACCAGA

1051 TGGAATGTGA GGAGGAGAAA GCTGCCACTC TTAGCTCAGA TACTTCTATT

1101 CAAGCTTCTG AACCCTTGCT TGATACGCAC ATAGTGAATG GAGAAAGAGA

1151 TGAAACTGCC ACAGCTCCTG CATCACCCAC AACAGATAGC TGTGATGGAA

1201 ATGCTTCTGA CAGTAGCTAC AGGACTCCAG GCATAGGCCC AGTGCTCCCC

1251 CTAGAAGAAA GAGGGGCAGA AACAGAAACC AAGGTACAAG AGAGGGAAAA

1301 TGGGGAAAGC CCTCTGGAAC TGGAGCAGCT GGACCAGCAC CATGAGATGA

1351 AGGTAGAGCA TGAGACTAGC TCATGAGCAG GGAAAACCCT GCCTATTCGA

1401 TTGTTGTCTT AAAACTCTTT ATTTATTGCA CCCCTGAAAT GTATGAATCA

1451 GATCACCCAC ACTGGCAGTT AAACGATTTT CAAGCTCTGG CTGCTGATTA

1501 GCATTTCCCC TATGCTCTAA GCAGATATTT CACTTTTTCT TTTCATGTAG

1551 TTTCTGTTAA TATCTCTGTT GTAATTTCAG GAGTCAGAAC AGTGTGGAAA

1601 CTTTAATATA GGAAATCCAC AAATGTATTG TTTTTACATA GAAAGAAAAT

1651 GTTCCTTGTT GCTCTAGATG TTGGTGCTGT ATCCCTAATA CTTACGGGCC

1701 AAGCAAGAAG AAATTGTATA ATCTTTGTTG TTCAGAAGTT TCTAATAGAA

1751 TAAATAGGCC TGTAAGATGA ACTTGCCACT AGTAAATGTT ACTTTTAAGG

1801 ACATGAATAT GGAAGTATTA AATTATTCAA CAGATAAAAA AAAAA

BLAST Results

No BLAST result Medlme entries

98334590:

Frabin, a novel FGDl-related actin filament-binding protein capable of changing cell shape and activating c-Jun N-termmal kinase.

Peptide information for frame 3

ORF from 810 bp to 1373 bp; peptide length: 188 Category: similarity to known protein Classification: Cell structure/motility

1 MFSCFLCILS FSSLSNYSDL KKESAVNLNA PRTPGRHGLT TTPQQKLLSQ 51 HLPQRQGNDT DKTQGAQTCV ANGVMAAQNQ MECEEEKAAT LSSDTSIQAS 101 EPLLDTHIVN GERDETATAP AΞPTTDSCDG NAΞDSSYRTP GIGPVLPLEE 151 RGAETETKVQ ERENGESPLE LEQLDQHHEM KVEHETSS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_72kl5, frame 3

TREMBL:AF038388_1 product: "actm-filament binding protein Frabin"; Rattus norvegicus actin-filament binding protein Frabin mRNA, complete eds., N = 1, Score = 428, P = 1.8e-39

>TREMBL:AF038388_1 product: "actm-filament binding protein Frabin"; Rattus norvegicus actm-filament binding protein Frabin mRNA, complete eds. Length = 766

HSPs:

Score = 428 (64.2 bits), Expect = 1.8e-39, P = 1.8e-39 Identities = 90/174 (51%), Positives = 115/174 (66%)

Query: 12 SSLSNYSDLKKESAVNLNAPRTPGRHGLTTTPQQKLLSQHLPQRQGNDTDKTQGAQTCVA 71

S LS+Y+D++K+S +NLN P+TP +HGLT+T QKL S PQ+Q D+D+ QG C+A Sbjct: 31 SVLSSYTDVQKDSTMNLNIPQTPRQHGLTSTTPQKLPSHKSPQKQEKDSDQNQGQHGCLA 90

Query: 72 NGVMAAQNQMECEEEKAATLSSDTSIQASEPLLDTHIVNGERDETATAPASPTTDSCDGN 131

NGV AAQ+QMECE EK A LS +T Q + D H++NG R+ET T AS T+S D N Sbjct: 91 NGVAAAQSQMECETEKEAALSPETDTQTAAASPDAHVLNGVRNETTTDSASSVTNSHDEN 150

Query: 132 ASDSSYRTPGIGPVLPLEERGAETETKVQERENGESPLELEQLDQHHEMKVEHE 185

A DSS RT G LP +E E ++QERENG S L LDQHHE+K +E Sbjct: 151 ACDSSCRTQGTDLGLPSKEGEPVIEAELQERENGLSTEGLNPLDQHHEVKETNE 204

Pedant information for DKFZphtes3_72kl5, frame 3

Report for DKFZphtes3_72kl5.3

[LENGTH] 188

[MW] 20388.32

[pi] 4.62

[HOMOL] TREMBL:AF038388_1 product: "actm-filament binding protein Frabin"; Rattus norvegicus actm-filament binding protein Frabin mRNA, complete eds. 2e-38

[KW] All_Alpha

[KW] SIGNAL_PEPTIDE 16

[KW] LOW_COMPLEXITY 12.77 %

SEQ MFΞCFLCILSFSSLSNYSDLKKESAVNLNAPRTPGRHGLTTTPQQKLLSQHLPQRQGNDT

SEG . xxxxxxxxxxxxx

PRD ccchhhhhcccccccccccccccccccccccccccccccccccchhhhhhhccccccccc

SEQ DKTQGAQTCVANGVMAAQNQMECEEEKAATLSSDTSIQASEPLLDTHIVNGERDETATAP SEG xxxxx

PRD ccccccceeecchhhhhhhhhhhhhhhhhhhccccceeecccccceeeeecccccccccc

SEQ ASPTTDSCDGNASDSSYRTPGIGPVLPLEERGAETETKVQERENGESPLELEQLDQHHEM

SEG xxxxx

PRD ccccccccccccccccccccccccccccccccchhhhhhhhhcccccchhhhhhhhhhhh

SEQ KVEHETSS

SEG

PRD hhhhhccc

(No Prosite data available for DKFZphtes3_72kl5.3) (No Pfam data available for DKFZphtes3 72kl5.3)

DKFZphtes3_72pl6

group: intracellular transport and trafficing

DKFZphtes3_72pl6 encodes a novel 796 ammo acid protein with very strong similarity to Mus musculus maternal-embryonic 3 (Mem3) gene.

Mem3 was isolated from a partial subtraction library of mouse unfertilized eggs and preimplantation embryos. Its transcript is abundant in the unfertilized egg and also actively transcribed from the newly formed zygotic genome. As Mem3, the novel protein is similar to yeast VPS (vacuolar protein sorting) 35. The null allele of VPS35 results in yeast in a differential defect in the sorting of vacuolar carboxypeptidase Y (CPY) , proteinase A (PrA) , proteinase B (PrB), and alkaline phosphatase (ALP).

The new protein can find application in modulation the sorting of proteins into different compartments . strong similarity to mouse MEM3 and yeast VPS35

Sequenced by DKFZ

Locus: /map="16pl3.3"

Insert length: 2707 bp

Poly A stretch at pos. 2697, no polyadenylation signal found

1 CTACGCGCGG GGCGGGTGCT GCTTGCTGCA GGCTCTGGGG AGTCGCCATG 51 CCTACAACAC AGCAGTCCCC TCAGGATGAG CAGGAAAAGC TCTTGGATGA

101 AGCCATACAG GCTGTGAAGG TCCAGTCATT CCAAATGAAG AGATGCCTGG

151 ACAAAAACAA GCTTATGGAT TCTCTAAAAC ATGCTTCTAA TATGCTTGGT

201 GAACTCCGGA CTTCTATGTT ATCACCAAAG AGTTACTATG AACTTTATAT

251 GGCCATTTCT GATGAACTGC ACTACTTGGA GGTCTACCTG ACAGATGAGT

301 TTGCTAAAGG AAGGAAAGTG GCAGATCTCT ACGAACTTGT ACAGTATGCT

351 GGAAACATTA TCCCAAGGCT TTACCTTTTG ATCACAGTTG GAGTTGTATA

401 TGTCAAGTCA TTTCCTCAGT CCAGGAAGGA TATTTTGAAA GATTTGGTAG

451 AAATGTGCCG TGGTGTGCAA CATCCCTTGA GGGGTCTGTT TCTTCGAAAT

501 TACCTTCTTC AGTGTACCAG AAATATCTTA CCTGATGAAG GAGAGCCAAC

551 AGATGAAGAA ACAACTGGTG ACATCAGTGA TTCCATGGAT TTTGTACTGC

601 TCAACTTTGC AGAAATGAAC AAGCTCTGGG TGCGAATGCA GCATCAGGGA

651 CATAGCCGAG ATAGAGAAAA AAGAGAACGA GAAAGACAAG AACTGAGAAT

701 TTTAGTGGGA ACAAATTTGG TGCGCCTCAG TCAGTTGGAA GGTGTAAATG

751 TGGAACGTTA CAAACAGATT GTTTTGACTG GCATATTGGA GCAAGTTGTA

801 AACTGTAGGG ATGCTTTGGC TCAAGAATAT CTCATGGAGT GTATTATTCA

851 GGTTTTCCCT GATGAATTTC ACCTCCAGAC TTTGAATCCT TTTCTTCGGG

901 CCTGTGCTGA GTTACACCAG AATGTAAATG TGAAGAACAT AATCATTGCT

951 TTAATTGATA GATTAGCTTT ATTTGCTCAC CGTGAAGATG GACCTGGAAT 1001 CCCAGCGGAT ATTAAACTTT TTGATATATT TTCACAGCAG GTGGCTACAG 1051 TGATACAGTC TAGACAAGAC ATGCCTTCAG AGGATGTTGT ATCTTTACAA 1101 GTCTCTCTGA TTAATCTTGC CATGAAATGT TACCCTGATC GTGTGGACTA 1151 TGTTGATAAA GTTCTAGAAA CAACAGTGGA GATATTCAAT AAGCTCAACC 1201 TTGAACATAT TGCTACCAGT AGTGCAGTTT CAAAGGAACT CACCAGACTT 1251 TTGAAAATAC CAGTTGACAC TTACAACAAT ATTTTAACAG TCTTGAAATT 1301 AAAACATTTT CACCCACTCT TTGAGTACTT TGACTACGAG TCCAGAAAGA 1351 GCATGAGTTG TTATGTGCTT AGTAATGTTC TGGATTATAA CACAGAAATT 1401 GTCTCTCAAG ACCAGGTGGA TTCCATAATG AATTTGGTAT CCACGTTGAT 1451 TCAAGATCAG CCAGATCAAC CTGTAGAAGA CCCTGATCCA GAAGATTTTG 1501 CTGATGAGCA GAGCCTTGTG GGCCGCTTCA TTCATCTGCT GCGCTCTGAG 1551 GACCCTGACC AGCAGTACTT GATTTTGAAC ACAGCACGAA AACATTTTGG 1601 AGCTGGTGGA AATCAGCGGA TTCGCTTCAC ACTGCCACCT TTGGTATTTG 1651 CAGCTTACCA GCTGGCTTTT CGATATAAAG AGAATTCTAA AGTGGATGAC 1701 AAATGGGAAA AGAAATGCCA GAAGATTTTT TCATTTGCCC ACCAGACTAT 1751 CAGTGCTTTG ATCAAAGCAG AGCTGGCAGA ATTGCCCTTA AGACTTTTTC 1801 TTCAAGGAGC ACTAGCTGCT GGGGAAATTG GTTTTGAAAA TCATGAGACA 1851 GTCGCATATG AATTCATGTC CCAGGCATTT TCTCTGTATG AAGATGAAAT 1901 CAGCGATTCC AAAGCACAGC TAGCTGCCAT CACCTTGATC ATTGGCACTT 1951 TTGAAAGGAT GAAGTGCTTC AGTGAAGAGA ATCATGAACC TCTGAGGACT 2001 CAGTGTGCCC TTGCTGCATC CAAACTTCTA AAGAAACCTG ATCAGGGCCG 2051 AGCTGTGAGC ACCTGTGCAC ATCTCTTCTG GTCTGGCAGA AACACGGACA 2101 AAAATGGGGA GGAGCTTCAC GGAGGCAAGA GGGTAATGGA GTGCCTAAAA 2151 AAAGCTCTAA AAATAGCAAA TCAGTGCATG GACCCCTCTC TACAAGTGCA 2201 GCTTTTTATA GAAATTCTGA ACAGATATAT CTATTTTTAT GAAAAGGAAA 2251 ATGATGCGGT AACAATTCAG GTTTTAAACC AGCTTATCCA AAAGATTCGA 2301 GAAGACCTCC CGAATCTTGA ATCCAGTGAA GAAACAGAGC AGATTAACAA 2351 ACATTTTCAT AACACACTGG AGCATTTGCG CTTGCGGCGG GAATCACCAG 2401 AATCCGAGGG GCCAATTTAT GAAGGTCTCA TCCTTTAAAA AGGAAATAGC 2451 TCACCATACT CCTTTCCATG TACATCCAGT GAGGGTTTTA TTACGCTAGG 2501 TTTCCCTTCC ATAGATTGTG CCTTTCAGAA ATGCTGAGGT AGGTTTCCCA 2551 TTTCTTACCT GTGATGTGTT TTACCCAGCA CCTCCGGACA CTCACCTTCA

2601 GGACCTTAAT AAAATTATTC ACTTGGTAAG TGTTCAAGTC TTTCTGATCA

2651 CCCCAAGTAG CATGACTGAT CTGCAATTTA AAATTCCTGT GATCTGTAAA 2701 AAAAAAA

BLAST Results

Entry AC007225 from database EMBLNEW:

Homo sapiens chromosome 16 clone 480G7, WORKING DRAFT SEQUENCE, 3ε unordered pieces .

Score = 1081, P = 2.8e-217, identities = 219/221 13 exons

Entry HS015146 from database EMBL: human STS WI-8848. Score = 2033, P = 2.9e-87, identities = 425/436

Medline entries

96327632:

Genetic mapping and embryonic expression of a novel, maternally transcribed gene Mem3.

97258867:

Endosome to Golgi retrieval of the vacuolar protein sorting receptor,

VpslOp, requires the function of the

VPS29, VPS30, and VPS35 gene products.

92360909:

Alternative pathways for the sorting of soluble vacuolar proteins in yeast: a vps35 null mutant missorts and secretes only a subset of vacuolar hydrolases.

10198044:

Distinct Domains within Vps35p Mediate the Retrieval of Two Different

Cargo Proteins from the Yeast

Prevacuolar/Endosomal Compartment

Peptide information for frame 3

ORF from 48 bp to 2435 bp; peptide length: 796 Category: strong similarity to known protein Classification: unset

1 MPTTQQSPQD EQEKLLDEAI QAVKVQSFQM KRCLDKNKLM DSLKHASNML 51 GELRTSMLSP KSYYELYMAI SDELHYLEVY LTDEFAKGRK VADLYELVQY 101 AGNIIPRLYL LITVGVVYVK SFPQSRKDIL KDLVEMCRGV QHPLRGLFLR 151 NYLLQCTRNI LPDEGEPTDE ETTGDISDSM DFVLLNFAEM NKLWVRMQHQ 201 GHSRDREKRE RERQELRILV GTNLVRLSQL EGVNVERYKQ IVLTGILEQV 251 VNCRDALAQE YLMECIIQVF PDEFHLQTLN PFLRACAELH QNVNVKNIII 301 ALIDRLALFA HREDGPGIPA DIKLFDIFSQ QVATVIQSRQ DMPSEDVVSL 351 QVSLINLAMK CYPDRVDYVD KVLETTVEIF NKLNLEHIAT SSAVSKELTR 401 LLKIPVDTYN NILTVLKLKH FHPLFEYFDY ESRKSMSCYV LSNVLDYNTE 451 IVSQDQVDSI MNLVSTLIQD QPDQPVEDPD PEDFADEQSL VGRFIHLLRS 501 EDPDQQYLIL NTARKHFGAG GNQRIRFTLP PLVFAAYQLA FRYKENSKVD 551 DKWEKKCQKI FSFAHQTISA LIKAELAELP LRLFLQGALA AGEIGFENHE 601 TVAYEFMSQA FSLYEDEISD SKAQLAAITL IIGTFERMKC FSEENHEPLR 651 TQCALAASKL LKKPDQGRAV STCAHLFWSG RNTDKNGEEL HGGKRVMECL 701 KKALKIANQC MDPSLQVQLF IEILNRYIYF YEKENDAVTI QVLNQLIQKI 751 REDLPNLESS EETEQINKHF HNTLEHLRLR RESPESEGPI YEGLIL

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_72pl6, frame 3

TREMBL :AF024504_3 gene: "A_TM017A05.7"; Arabidopsis thaliana BAC TM017A05., N = 2, Score = 927, P = 1.9e-162 PIR:S56936 vacuolar protem-sortmg protein VPS35 - yeast (Saccharomyces cerevisiae), N = 3, Score = 826, P = 1.5e-116

TREMBL:MM47024_1 gene: "Mem3"; product: "MEM3"; Mus musculus maternal-embryonic 3 (Mem3) mRNA, complete eds., N = 1, Score = 3376, P = 0

TREMBL:S42186_1 gene: "VPS35"; product: "Vps35p"; VPS35=vacuolar protein sorting [Saccharomyces cerevιsιae=yeast, Genomic, 3790 nt] , N = 3, Score = 813, P = 4.4e-115

>TREMBL:MM47024_1 gene: "Mem3"; product: "MEM3"; Mus musculus maternal-embryonic 3 (Mem3) mRNA, complete eds. Length = 754

HSPs:

Score = 3376 (506.5 bits). Expect = O.Oe+00, P = O.Oe+00 Identities = 666/721 (92%), Positives = 682/721 (94%)

Query: 78 EVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMC 137

+VYLTDEFAKG ++ADLYELVQY+GNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMC Sbjct: 34 KVYLTDEFAKGERLADLYELVQYSGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMC 93

Query: 138 RGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRM 197

RGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRM Sbjct: 94 RGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRM 153

Query: 198 QHQGHSRDREKRERERQELRILVGTNLVRLSQLEG-VNVERYKQIVLTGILEQVVNCRDA 256

QHQGHSRDREKRERERQELRILVGTNLV L+ + +QIVLTGILEQVVNCRDA

Sbjct: 154 QHQGHSRDREKRERERQELRILVGTNLVALTLVSWRCKCGTLQQIVLTGILEQVVNCRDA 213

Query: 257 LAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGP 316

LAQE MECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHRE P Sbjct: 214 LAQEISMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREMEP 273

Query: 317 GIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETT 376

GIPA++KLFDIFSQQVATVIQSR+DMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETT Sbjct: 274 GIPAELKLFDIFSQQVATVIQSRRDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETT 333

Query: 377 VEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESR—K 434

VEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYES K Sbjct: 334 VEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESSPGK 393

Query: 435 SMΞCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRF 494

SMΞCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRF Sbjct: 394 SMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRF 453

Query: 495 IHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWE 554

IHLLRΞ+DPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSK + Sbjct: 454 IHLLRSDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKWMTSGK 513

Query: 555 KKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLY 614

+ ++ F HQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLY Sbjct: 514 RNARRYFHLPHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLY 573

Query: 615 EDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAAΞKLLKKPDQGRAVSTCA 674

EDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRT+CALAASKLLKKPDQ C Sbjct: 574 EDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTECALAASKLLKKPDQAEREHMCT 633

Query: 675 HLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKE 734

L WSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKE Sbjct: 634 SL-WSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKE 692

Query: 735 NDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGL 794

NDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLR RRESPESEGPIYEGL Sbjct: 693 NDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRTRRESPESEGPIYEGL 752

Query: 795 IL 796

IL Sbjct: 753 IL 754

Pedant information for DKFZphtes3_72pl6, frame 3

Report for DKFZphtes3_72pl6.3

[LENGTH] 796 [MW] 91723.67

[pi] 5.32

[HOMOL] TREMBL:MM47024_1 gene: "Mem3"; product: "MEM3"; Mus musculus maternal-embryonic

3 (Mem3) mRNA, complete eds. 0.0

[FUNCAT] 30.25 vacuolar and lysosomal organization [S. cerevisiae, YJL154c] le-110

[FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YJL154c] le-110

[FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YJL154c] le-110

[FUNCAT] 30.22 endosomal organization [S. cerevisiae, YJL154c] le-110

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YJL154c] le-110

[FUNCAT] 30.08 organization of golgi [S. cerevisiae, YJL154c] le-110

[FUNCAT] 09.07 biogenesis of endoplasmatic reticulum [S. cerevisiae, YJL154c] le-110

[BLOCKS] BL01092Q

[PIRKW] yeast vacuole le-108

[PIRKW] membrane protein le-108

[KW] TRANSMEMBRANE 1

[KW] LOW COMPLEXITY 5.40 %

SEQ MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDSLKHASNMLGELRTSMLSP

SEG

PRD cccccccccchhhhhhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhcccc

MEM

SEQ KSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVK

SEG

PRD cceeeeehhhhhhhhhhhhhhhhhhhchhhhhhhhhhhhhhcccccccceeeeeceeeee

MEM MMMMMMMMMMMMMM

SEQ SFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSM

SEG xxxxxxxxxxxxxx

PRD ecccchhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhcccccccccccccccccccch

MEM MMMMMMMMMM

SEQ DFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQ

SEG xxxxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhccchhhhhhhhccchhhhhh

MEM

SEQ IVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIII

SEG

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccchhhhhhhhhhhhccccchhhhhh

MEM

SEQ ALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMK

SEG

PRD hhhhhhhhhhhhcccccccccchhhhhhhhhhhhhhhhccccccccchhhhhhhhhhhhh

MEM

SEQ CYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKH

SEG

PRD cccccccchhhhhhhhhhhhhccchhhhhhccchhhhhhhhhccccccchhhhhhhhhhh

MEM

SEQ FHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPD

SEG xxxxxxxxxxxx

PRD hhhheeecccchhhhhhhhhhhhccccceeehhhhhhhhhhhhhhhhhhccccccccccc

MEM

SEQ PEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLA

SEG xxx

PRD ccccchhhhhhhhhhhhhhccccchhhhhhhhhhhhhcccccceeeeeccchhhhhhhhh

MEM

SEQ FRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHE

SEG

PRD hhhhhcccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccc

MEM

SEQ TVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKL

SEG

PRD eeeeehhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhcccccccchhhhhhhhhhhh

MEM

SEQ LKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLF

SEG

PRD hhcccceeeeecccccccccccccccccccccchhhhhhhhhhhhhhhhhhchhhhhhhh

MEM

SEQ IEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLR SEG

PRD hhhhhhhhhhhccccceeeeehhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhh

MEM

SEQ RESPESEGPIYEGLIL

SEG

PRD hhcccccccceeeccc

MEM

(No Prosite data available for DKFZphtes3_72pl6 . 3 ) (No Pfam data available for DKFZphtes3_72pl 6. 3 )

DKFZphtes3_7b22

group: cell structure and motility

DKFZphtes3_7b22 encodes a novel 443 amino acid protein with weak similarity to paramyosins.

The novel protein is related to paramyosin, a major structural component of thick filaments and invertebrate muscle. Paramyosins are promising antigens for immunization against several parasites, such as Schistosoma mansom .

The new protein can find application in modulating cell adhesion/motility and membrane/cyto skeleton structure and dynamic. similarity to paramyosins complete cDNA, complete eds, few EST hits

Sequenced by BMFZ

Locus: /map="3"

Insert length: 2291 bp

Poly A stretch at pos. 2241, polyadenylation signal at pos. 2213

1 GGAAGAAAGG CTAGCGGGCG TTGGCCGTAT GTGGGTGTCT TGAGGCAGTT

51 TTTCAGTTCT TTCATTTACC AAAGTGACAT GCACCTACTA GGTGCCAGGT

101 GTTTAGACGT ACATACAACC CTCTGCAAAA TCTTTCAGTG TAGTCCTCTG

151 TATGAAAAGT TTCCAGCCAA GAATTGCCAC TGCACCTGAG ATAAGGGGGA

201 TCCTGGCCAT TAAGGAAACC TTGCCTTCGA AACTGAGCCG TGAGGAACTA

251 TACAAAATGG GAAATTGGGA CAAATCCCAG TGGCTCATGA CACTAAGAAG

301 TAAAATTACG AACTCACTGA GCTGGAAGTC ATTCAACGGG AATTGAATAG

351 GTAACTGCAC TTTTGTGAGA TTATAAATAT ACCACGGAGG GTAACGAAGC

401 TACAGAAGAA TGGAAGAAGA CAGCCTGGAA GACTCAAACC TTCCTCCAAA

451 AGTTTGGCAT TCTGAGATGA CGGTGTCAGT GACAGGCGAA CCACCTAGTA

501 CCGTAGAAGA AGAAGGAATA CCTAAAGAAA CAGACATAGA AATCATCCCA

551 GAAATCCCGG AAACTCTAGA GCCACTGTCC CTTCCAGATG TGCTGAGGAT

601 CTCGGCAGTT CTGGAGGACA CCACAGACCA GCTCTCTATT CTGAACTACA

651 TCATGCCCGT TCAGTACGAA GGGAGACAGA GCATCTGCGT GAAAAGCAGA

701 GAAATGAATC TAGAAGGAAC GAATCTAGAC AAACTTCCAA TGGCCTCAAC

751 AATCACAAAA ATACCCAGTC CGTTAATAAC TGAGGAAGGA CCCAACTTGC

801 CAGAAATCAG ACACAGAGGC CGGTTCGCTG TGGAGTTTAA CAAAATGCAG

851 GATCTTGTCT TCAAAAAACC TACAAGGCAG ACCATCATGA CTACGGAGAC

901 ACTGAAGAAA ATTCAGATTG ATAGGCAGTT TTTCAGCGAT GTGATTGCAG

951 ATACCATTAA GGAGTTGCAA GATTCGGCCA CTTACAACAG TCTCCTGCAA

1001 GCTTTGAGCA AAGAGAGGGA AAACAAAATG CATTTCTATG ACATCATTGC

1051 CAGGGAGGAA AAAGGAAGAA AACAGATAAT ATCACTTCAA AAACAGCTAA

1101 TTAATGTCAA AAAGGAATGG CAATTTGAAG TCCAGAGTCA GAATGAGTAT

1151 ATTGCTAACC TCAAGGACCA ACTGCAAGAG ATGAAGGCAA AATCCAACTT

1201 GGAGAATCGC TACATGAAAA CCAATACCGA GCTGCAGATT GCCCAGACCC

1251 AGAAAAAGTG TAACAGAACA GAGGAACTCT TGGTGGAAGA GATTGAGAAA

1301 CTCAGGATGA AAACCGAAGA AGAGGCCCGG ACTCATACAG AGATTGAAAT

1351 GTTCCTTAGA AAGGAGCAGC AGAAACTTGA GGAGAGGCTG GAGTTCTGGA

1401 TGGAGAAATA CGATAAGGAC ACAGAAATGA AACAGAATGA ACTAAATGCT

1451 CTCAAAGCCA CAAAGGCCAG TGACTTAGCA CACCTTCAAG ACCTGGCAAA

1501 GATGATAAGA GAGTATGAAC AGGTCATCAT TGAAGATCGT ATAGAAAAGG

1551 AGAGGAGCAA GAAGAAGGTA AAACAGGATC TCTTGGAATT AAAGAGCGTT

1601 ATAAAGCTCC AGGCCTGGTG GCGAGGCACT ATGATACGGA GAGAAATTGG

1651 TGGTTTCAAG ATGCCTAAAG ACAAAGTTGA TAGCAAGGAT TCAAAAGGCA

1701 AAGGTAAAGG CAAGGATAAG AGGAGAGGCA AGAAGAAGTG ACCAAGTTCT

1751 CTTTTGTGTT TTCTGCTGGT ATTCTGGAGG TGGGAAGGAC TTGGAGAGTT

1801 AAGAAACACC TGGTACCTCA AAGATGACTC ATCTACAGGT TGTTTCCTAT

1851 TGAGACTTTC CCAGGGAAGC CTGATTTCAC TTTGCCTGTT AATTTCACTC

1901 TGCCTGTTAG GTGGGTTTTC AAACCCTGAT TTAGGATTAC ACCATTGACT

1951 TAGGGCTTCC TCATACCTTG CTGGGAAGAA GTTTCTAGTA GTCCTGTGAA

2001 GATTCATTCT TCTTGCTCTT TCTCAGCAGA ACAAAGGAGT TCACTGGCTT

2051 AGCTACAGTG ACGCATTGAA ACTTGAGTAA TTCCTGTAAT GTCAGATTTT

2101 GATTTTACCC AATTTGTCTG TAGTGAAAAA ACTCTTATGA GCAAAAGTAT

2151 TCAGTAGGAA TTACAATATG ATGTTATTAG CTGTCCAGCA TAATATATAC

2201 ACAGCAAAGT TTTAATAAAT GTTGGTTCCT GCCTGCCTTT TAAAAAAAAA

2251 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA A

BLAST Results

Entry G36731 from database EMBL: SHGC-52923 Human Homo sapiens STS cDNA. Score = 2262, P = 1.3e-97, identities = 462/468

Medline entries

No Medlme entry

Peptide information for frame 2

ORF from 410 bp to 1738 bp; peptide length: 443 Category: similarity to known protein

1 MEEDSLEDSN LPPKVWHSEM TVSVTGEPPS TVEEEGIPKE TDIEIIPEIP 51 ETLEPLSLPD VLRISAVLED TTDQLSILNY IMPVQYEGRQ SICVKSREMN 101 LEGTNLDKLP MASTITKIPS PLITEEGPNL PEIRHRGRFA VEFNKMQDLV 151 FKKPTRQTIM TTETLKKIQI DRQFFSDVIA DTIKELQDSA TYNSLLQALS 201 KERENKMHFY DIIAREEKGR KQIISLQKQL INVKKEWQFE VQSQNEYIAN 251 LKDQLQEMKA KSNLENRYMK TNTELQIAQT QKKCNRTEEL LVEEIEKLRM 301 KTEEEARTHT EIEMFLRKEQ QKLEERLEFW MEKYDKDTEM KQNELNALKA 351 TKASDLAHLQ DLAKMIREYE QVIIEDRIEK ERSKKKVKQD LLELKΞVIKL 401 QAWWRGTMIR REIGGFKMPK DKVDSKDSKG KGKGKDKRRG KKK

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_7b22, frame 2

SWISSPROT :MYSP_BRUMA PARAMYOSIN., N = 1, Score = 158, P = 5.8e-08

PIR:A44972 paramyosin - nematode (Dirofilaria lmmitis) (fragment), N = 1, Score = 157, P = 7. le-08

SWISSPROT:MYSP_ONCVO PARAMYOSIN., N = 1, Score = 157, P = 7.4e-08

PIR:S52537 emm L 15 protein - Streptococcus pyogenes, N = 1, Score = 151, P = 8.6e-08

>SWISSPROT:MYSP_BRUMA PARAMYOSIN. Length = 880

HSPs:

Score = 158 (23.7 bits), Expect = 5.8e-08, P = 5.8e-08 Identities = 66/259 (25%), Positives = 125/259 (48%)

Query: 142 EFNKMQDLVFKKPTRQTIMTTETLKKIQIDRQFFSDVIADTIKELQDSATYNSLLQALSK 201

+ K + L K R T E K++ + +D +A + LQ A N LL+ + Sbjct: 169 QLKKDKHLAEKAAERFEAQTVELSNKVEDLNRHVND-LAQQRQRLQ—AENNDLLKEIHD 225

Query: 202 ER ENKMHF-YDIIAREEKGRKQIISLQKQLINVKKEWQFEVQSQNEYIANLKDQLQE 257

++ +N H Y + + E+ R+++ +++ ++ + +VQ + + + D+ E Sbjct: 226 QKVQLDNLQHVKYQLAQQLEEARRRLEDAERERSQLQAQLH-QVQLELDSVRTALDE—E 282

Query: 258 MKAKSNLENRYMKTNTELQIAQTQKKCNRTEELLVEEIEKLRMKT-EEEARTHTEIEMFL 316

A++ E++ NTE I Q + K + L EE+E LR K +++A +IE+ L Sbjct: 283 SAARAEAEHKLALANTE—ITQWKSKFDAEVALHHEEVEDLRKKMLQKQAEYEEQIEIML 340

Query: 317 RKEQQ—KLEERLEFWMEKYDKDTEMKQNELNALKATKASDLAHLQDLAKMIREYEQVII 374

+K Q K + RL+ +E D E QN + L+ K + L K + E + I Sbjct: 341 QKISQLEKAKSRLQSEVEVLIVDLEKAQNTIAILERAK EQLEKTVNELKVRID 393

Query: 375 EDRIEKERSKKKVKQDLLELKSVIKL 400

E +E E ++++ + L EL+ + L Sbjct: 394 ELTVELEAAQREARAALAELQKLKNL 419

Score = 118 (17.7 bits), Expect = 1.3e-03, P = 1.3e-03 Identities = 54/231 (23%), Positives = 108/231 (46%)

Query: 181 DTIKELQDSATYNSLLQ ALSKERENKMHFYDIIAREEKG-RKQIISLQKQLINVKK 235

D +KE+ D LQ L+++ E + RE + Q+ +Q +L +V+ Sbjct: 218 DLLKEIHDQKVQLDNLQHVKYQLAQQLEEARRRLEDAERERSQLQAQLHQVQLELDSVRT 277 Query: 236 EWQFE—VQSQNEY-IANLKDQLQEMKAKSNLENRYMKTNTE-LQIAQTQKKCNRTEELL 291

E +++ E+ +A ++ + K+K + E E L+ QK+ E++

Sbjct: 278 ALDEESAARAEAEHKLALANTEITQWKSKFDAEVALHHEEVEDLRKKMLQKQAEYEEQIE 337

Query: 292 VEEIEKLRMKTEEEARTHTEIEMF LRKEQQKLE—ERLEFWMEKYDKDTEMKQNELN 346

+ ++K+ + ++R +E+E+ L K Q + ER + +EK + +++ +EL

Sbjct: 338 IM-LQKISQLEKAKΞRLQSEVEVLIVDLEKAQNTIAILERAKEQLEKTVNELKVRIDELT 396

Query: 347 A-LKATKASDLAHLQDLAKMIREYEQVIIEDRIEKERSKKKVKQDLLELKSVI 398

L+A + A L +L K+ YE+ + E + R KK++ DL E K +

Sbjct: 397 VELEAAQREARAALAELQKLKNLYEKAV-EQKEALARENKKLQDDLHEAKEAL 448

Score . = 107 (16.1 bits), Expect = 2.1e-02, P = 2.1e-02

Identities ^:= 49/279 (17%), Positives = 124/279 (44%)

Query: 123 ITEEGPNLPEIRHRGRFAV-EFNKMQDLVFKKPTRQTIMTTETLKKIQIDRQFFSDVIAD 181 I E L + R A+ E K+++L K ++ + E KK+Q D + +AD

Sbjct: 392 IDELTVELEAAQREARAALAELQKLKNLYEKAVEQKEALAREN-KKLQDDLHEAKEALAD 450

Query: 182 TIKELQDSATYNSLLQALSKERENKMHFYDIIAREEKGRKQ—IISLQKQLINVKKEWQF 239

++L + N+ L +E + + + R+ + R Q + LQ+ I +++ Q

Sbjct: 451 ANRKLHELDLENARLAGEIRELQTALKESEAARRDAENRAQRALAELQQLRIEMERRLQE 510

Query: 240 EVQSQNEYIANLKDQLQEMKAKSNLENRYMKTNTELQIAQTQKKCNRTE-ELLVEEIEKL 298 + + N++ ++ + A L + + E+ + + + E E+ V+ + +

Sbjct: 511 KEEEMEALRKNMQFEIDRLTAA—LADAEARMKAEISRLKKKYQAEIAELEMTVDNLNRA 568

Query: 299 RMKTEEEARTHTEIEMFLRKEQQKLEERLEFWMEKYDKDTEMKQNELNALKATKASDLAH 358

++ ++ + +E L+ + + +L+ +++Y + Q +++AL A + +

Sbjct: 569 NIEAQKTIKKQSEQLKILQASLEDTQRQLQQTLDQY ALAQRKVSALSA-ELEECKV 623

Query: 359 LQDLAKMIREYEQVIIEDRIEKERSKKKVKQDLLELKSVIKLQ 401

D A R+ ++ +E+ + V +L +K+ ++ +

Sbjct: 624 ALDNAIRARKQAEIDLEEANGRITDLVSVNNNLTAIKNKLETE 666

Pedant information for DKFZphtes3_7b22, frame 2

Report for DKFZphtes3_7b22.2

[LENGTH] 443

[MW] 51917.95

[pl] 6.18

[HOMOL] PIR:S28589 trichohyalm - rabbit 2e-08

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDL058w] 7e-07

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL058w]

7e-07

[FUNCAT] 1 genome replication, transcription, recombination and repair [M. jannaschii, MJ1322] 5e-06

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YPR141c] le-05

[FUNCAT] 03.13 meiosis [S. cerevisiae, YPR141c] le-05

[FUNCAT] 11.01 stress response [S. cerevisiae, YPR141c] le-05

[FUNCAT] 03.07 pheromone response, matmg-type determination, sex-specific proteins

[S. cerevisiae, YPR141c] le-05 [FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YPR141c] le-05 [FUNCAT] 09.10 nuclear biogenesis [S. cerevisiae, YPR141c] le-05 [FUNCAT] 30.05 organization of centrosome [S. cerevisiae, YPR141c) le-05 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YPR141c] le-05 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YOR216c] 3e-05 [FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YKR095w] 6e-05 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKR095w] 6e-05 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YER008c] le-04 [FUNCAT] 08.16 extracellular transport [S. cerevisiae, YER008c] le-04 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YER008c] le-04 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YDR356w] 2e-04 [FUNCAT] 08.01 nuclear transport [S. cerevisiae, YDL207w] 4e-04 [FUNCAT] 04.07 rna transport [S. cerevisiae, YDL207w] 4e-04 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylati an, farnesylation and processing) [S. cerevisiae, YKL201c] 5e-04 [EC] 3.6.1.32 Myosin ATPase 3e-08 [PIRKW] phosphotransferase 6e-06 [PIRKW] citrulline 8e-06 [PIRKW] tandem repeat le-07 [PIRKW] heart 6e-06 [PIRKW] polymorphism 4e-06 [PIRKW] ser ne/threomne-specific protein kinase 6e-06 [PIRKW] DNA binding 8e-08 [PIRKW] muscle contraction le-07

[PIRKW] actm binding 3e-08

[PIRKW] ATP 3e-08

[PIRKW] thick filament le-07

[PIRKW] phosphoprotein 3e-08

[PIRKW] glycoprotein 4e-06

[PIRKW] skeletal muscle le-07

[PIRKW] calcium binding 8e-06

[PIRKW] alternative splicing 3e-08

[PIRKW] coiled coil 3e-08

[PIRKW] P-loop 3e-08

[PIRKW] heptad repeat 4e-06

[PIRKW] methylated amino acid 3e-08

[PIRKW] basement membrane 4e-06

[PIRKW] cardiac muscle 6e-06

[PIRKW] extracellular matrix 4e-06

[PIRKW] hydrolase 3e-08

[PIRKW] membrane protein 4e-06

[PIRKW] EF hand 8e-06

[PIRKW] cytoskeleton 8e-06

[PIRKW] hair 8e-06

[SUPFAM] myosin heavy chain 3e-08

[SUPFAM] unassigned Ser/Thr or Tyr-specific protein kinases 6e-06

[SUPFAM] calmodulin repeat homology 8e-06

[SUPFAM] myosin motor domain homology 3e-08

[SUPFAM] trichohyalm 8e-06

[SUPFAM] protein kinase homology 6e-06

[PROSITE] AMIDATION 2

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE) CK2_PHOSPHO_SITE 12

[PROSITE] TYR_PHOSPHO_SITE 2

[PROSITE] PKC_PHOSPHO_SITE 4

[PROSITE] ASN_GLYCOSYLATION

[KW] All_Alpha

[KW] LOW COMPLEXITY 10.61 %

SEQ MEEDSLEDSNLPPKVWHSEMTVSVTGEPPSTVEEEGIPKETDIEIIPEIPETLEPLSLPD SEG xxxxxxxxxxxxxxxxxxxxxxx .

PRD cccccccccccccccccceeeeeccccccceeeeecccccceeeeeeccccccccccccc

SEQ VLRISAVLEDTTDQLSILNYIMPVQYEGRQSICVKSREMNLEGTNLDKLPMASTITKIPS SEG PRD chhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ PLITEEGPNLPEIRHRGRFAVEFNKMQDLVFKKPTRQTIMTTETLKKIQIDRQFFSDVIA SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ DTIKELQDSATYNSLLQALSKERENKMHFYDIIAREEKGRKQIISLQKQLINVKKEWQFE SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ VQSQNEYIANLKDQLQEMKAKΞNLENRYMKTNTELQIAQTQKKCNRTEELLVEEIEKLRM SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ KTEEEARTHTEIEMFLRKEQQKLEERLEFWMEKYDKDTEMKQNELNALKATKASDLAHLQ SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ DLAKMIREYEQVIIEDRIEKERSKKKVKQDLLELKSVIKLQAWWRGTMIRREIGGFKMPK SEG x PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccc

SEQ DKVDSKDSKGKGKGKDKRRGKKK SEG xxxxxxxxxxxxxxxxxxxxxxx

PRD CCCCCCCCCCCCCCCCCCCCCCC

Prosite for DKFZphtes3_7b22.2

PS00001 285- ->289 ASN GLYCOSYLATION PDOC00001

PS00004 152- ->156 CAMP PHOSPHO SITE PDOC00004

PS00005 164- ->167 PKC PHOSPHO SITE PDOC00005

PS00005 182- ->185 PKC PHOSPHO SITE PDOC00005

PS00005 280- ->283 PKC PHOSPHO SITE PDOC00005

PS00005 383- ->386 PKC PHOSPHO SITE PDOC00005

PS00006 5->9 CK2 PHOSPHO SITE PDOC00006

PS00006 30->34 CK2 PHOSPHO SITE PDOC00006 PS00006 41->45 CK2 PHOSPHO SITE PDOC00006

PS00006 57->61 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 104->108 CK2 PHOSPHO ^"SITE PDOC00006

PS00006 182->186 CK2 PHOSPHO SITE PDOC00006

PS00006 243->247 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 262->266 CK2 PHOSPHO^{" "}SITE PDOC00006

PΞ00006 271->275 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 302->306 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 308->312 CK2 PHOSPHO^{" "}SITE PDOC00006

PS00006 310->314 CK2 PHOSPHO SITE PDOC00006

PS00007 261->269 TYR PHOSPHO^{" "}SITE PDOC00007

PS00007 184->193 TYR PHOSPHO^{" "}SITE PDOC00007

PS00009 218->222 AMIDATION PDOC00009

PS00009 439->443 AMIDATION PDOC00009

(No Pfam data available for DKFZphtes3_7b22.2 )

DKFZphtes3_7dl7

group: testes derived

DKFZphtes3_7dl7 encodes a novel 633 amino acid protein with weak similarity to human KIAA0454.

Pfam predicts a TNFR/NGFR cysteme-rich region.

No informative BLAST results; No predictive prosite or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . similarity to KIAA0454 complete cDNA, complete eds, EST hits

Sequenced by BMFZ

Locus : unknown

Insert length: 3608 bp

Poly A stretch at pos. 3587, polyadenylation signal at pos. 3570

1 GGGAAGTTAC GGCGAAGTCC ACCCAGCGTT TCTCAGGCAA TCTGAAGGCA 51 AATCCTGTTT AGACCCAGGC GAAGGTTCCT GGTGACCCAG GCTCTCACCA

101 GCCAATTGTC CCTTGCCGTC CTCCTGAGGG TATCTGGAGC TTCAGTGCTG

151 TGTGCTCTTG GCCTCCACAC TGGGGATGCC ACTGACTCCC ACTGTCCAGG

201 GCTTCCAGTG GACTCTCCGA GGCCCTGATG TAGAAACTTC CCCATTCGGT

251 GCACCAAGAG CAGCCTCACA TGGTGTGGGC CGACATCAAG AGCTGCGAGA

301 TCCAACAGTC CCTGGCCCCA CCTCTTCTGC CACAAACGTC AGCATGGTGG

351 TATCTGCCGG CCCTTGGTCC GGTGAGAAGG CAGAGATGAA CATTCTAGAA

401 ATCAACAAGA AATCGCGCCC CCAGCTGGCA GAGAACAAAC AGCAGTTCAG

451 AAACCTCAAA CAGAAATGTC TTGTAACTCA AGTGGCCTAC TTCCTGGCCA

501 ACCGGCAAAA TAATTACGAC TATGAAGACT GCAAAGACCT CATAAAATCT

551 ATGCTGAGGG ATGAGCGGCT GCTCACAGAA GAGAAGCTTG CAGAGGAGCT

601 CGGGCAAGCT GAGGAGCTCA GGCAATATAA AGTCCTGGTT CACTCTCAGG

651 AACGAGAGCT GACCCAGTTA AGGGAGAAGT TACAGGAAGG GAGAGATGCC

701 TCCCGCTCAT TGAATCAGCA TCTCCAGGCC CTCCTCACTC CGGATGAGCC

751 GGACAACTCC CAGGGACGGG ACCTCCGAGA ACAGCTGGCT GAGGGATGTA

801 GGCTGGCACA GCACCTCGTC CAAAAGCTCA GCCCAGAAAA TGATGACGAT

851 GAGGATGAAG ATGTTAAAGT TGAGGAGGCT GAGAAAGTAC AGGAATTATA

901 TGCCCCCAGG GAGGTGCAGA AGGCTGAAGA AAAGGAAGTC CCTGAGGACT

951 CACTGGAGGA GTGTGCCATC ACTTGTTCAA ATAGCCACCA CCCTTGTGAG 1001 TCCAACCAGC CTTACGGGAA CACCAGAATC ACATTTGAGG AAGACCAAGT 1051 CGACTCAACT CTCATTGACT CATCCTCTCA TGATGAATGG TTGGATGCTG 1101 TATGCATTAT CCCAGAAAAT GAAAGTGATC ATGAGCAAGA GGAAGAAAAA 1151 GGGCCAGTGT CTCCCAGGAA TCTGCAGGAG TCTGAAGAGG AGGAAGCCCC 1201 CCAGGAGTCC TGGGATGAAG GTGATTGGAC TCTCTCAATT CCTCCTGACA 1251 TGTCTGCCTC ATACCAGTCT GACAGGAGCA CCTTTCACTC AGTAGAGGAA 1301 CAGCAAGTCG GCTTGGCTCT TGACATAGGC AGACATTGGT GTGATCAAGT 1351 GAAAAAGGAG GACCAAGAGG CCACAAGTCC CAGGCTCAGC AGGGAGCTGC 1401 TGGATGAGAA AGAGCCTGAA GTCTTGCAGG ACTCACTGGA TAGATTTTAT 1451 TCAACTCCTT TTGAGTACCT GGAACTGCCT GACTTATGCC AGCCCTACAG 1501 AAGTGACTTT TACTCATTGC AGGAACAACA CCTTGGCTTG GCTCTTGACT 1551 TGGACAGAAT GAAAAAGGAC CAAGAAGAGG AAGAAGACCA AGGCCCACCA 1601 TGCCCCAGGC TCAGCAGAGA GCTGCCGGAG GTAGTAGAGC CTGAGGACTT 1651 GCAGGACTCA CTGGATAGAT GGTATTCGAC TCCTTTCAGT TATCCAGAAC 1701 TGCCTGATTC ATGCCAGCCC TACGGAAGTT GCTTTTACTC ATTGGAGGAA 1751 GAACACGTTG GCTTTTCTCT TGACGTGGAT GAAATTGAAA AGTACCAAGA 1801 AGGGGAAGAA GATCAAAAGC CACCATGCCC CAGGCTCAAC GAGGTGCTGA 1851 TGGAAGCAGA AGAGCCTGAA GTCTTGCAGG ACTCACTGGA TAGATGTTAT 1901 TCGACTACTT CAACTTACTT TCAACTACAT GCCTCATTCC AGCAGTACAG 1951 AAGTGCCTTT TACTCATTTG AGGAACAGGA CGTCAGCTTG GCCCTTGACG 2001 TGGACAATAG GTTTTTTACT TTGACAGTGA TAAGGCACCA CCTGGCCTTC 2051 CAGATGGGAG TCATATTCCC ACACTAAGCA GCCCTTACTA AGCTGAGAGA 2101 TGTCATTGCT GCAGGCAGGA CCTATAGGCA CATGTAGGTT TGAATGAAAC 2151 TGTAGTTCCC TTTGGAAGCC CAGTCATAGG ATGGGAAAGT GGGCATGGCT 2201 CTATTCCTAT TCTCAGACCA TGCCAGTGGC CACCTGTGCT CAGTCTGAAG 2251 ACGTTGGACC CAAGTTAGGT GTGACACGTT CACACGACTA TGTAGCACAT 2301 GCCGGGAGTG ATCTGCCAGA CATTCTAATT TGAACCAGAT ATCTCTGGGT 2351 AGCTACAAAG TTCCTCAGGG GTTTCATTTT GCAGGCATGT CTCTGAGCTT 2401 CTATACCTGC TCAAGGTCAG TGTCATCTTT GTGTTTAGCT CATCCAAAGG 2451 TGTTACCCTG GTTTCATTGA ACCTAACCCC ATTCTTTGTA TCTTCAGTGT 2501 TGGTTTGTTT TAGCTGATCC ATCTGTAACA CAGGAGGGAT CCTTGGCTGA 2551 GGATTGTATT TCAGAACCAC TGACTGCTCT TGACAGTTGT TAACCCACTA 2601 GGCTCCTTTG AGTAGAGAAG CCATAGTCCT TCAGCCTCCA ATTGATATCA 2651 ATACTTAGGA AGACCACAGC TAGACGGACA AACAGCATTG GGAGGCCTTA 2701 GTCCTGCTCC TTTCAATTCC ATCCTGTAAA GAACAGGAGT CAGGAGCCGC

2751 TGGCAAGAGA CAGCATGTCA CCTGGGACTC TGCCAGTGCA GAATATGAAC

2801 AATGCCATGT TCTTGCAGAA AATGCTTAGC CTGAGTTTCA TAGGAGGTAA

2851 TCACCAGACA ACTGCAGAAT GTAGAACACT GAGCAGGACA ACTGACCTGT

2901 CTCCTTCACA CAGTCCACGT CACCACGAAT CACACAACAA AAAGGAGGAG

2951 AGATATTTTG GGTTCAGAAG AAGTAAATGA TAATGTAGCT ACATTTCTTT

3001 AGTTATTTTG AACCCCAAAT ATTTCCTCAT CTTTTTGTTG TTGTCATTGA

3051 TTTTGGTGAC ATGGACTTGT TTGTAGAGGA CAGGTCAGCT GTCTGGCTCA

3101 ATGGTCTACA TTCTGAAGTT GTCTGAAAAT GTCTTCATGA TTAAATTCAG

3151 CCTAAACGTT TCATCAAGAA CACTACAGAG TCGATACTGT GAGTTTCCAA

3201 CCTCAGCCCA TCTGTGGGCA GAGAAGGTCT AGTTTGTCCA TCAGCATTAT

3251 CATGATATCA GGACTGGTTA CTTGGTTAAG GAGGGGTCTA GGAGATCTGT

3301 CCCTTTTAGA GACACCTTAC TTATGATGAA GTATTTGGGA GAGTGGTTTT

3351 TCAAAGTAGA AATGTCCTGT ATTCCAGTGA TCATCCTCTA AACGTTTTAT

3401 CATTTATTAA TCATCCCTGC CTGTGTCTAT TATTATATTC ATATCTCTAC

3451 GCTGGAAATT TGCTGCCTCA ATGTTTACTG TGCCTTTGTT TTTGCTAGTG

3501 TGTGTTGTTG AAAAAAAAAC ATTCTCTGCC TGAGTTTTAA TTTTTGTCCA

3551 AAGTTATTTT AATCTATACA ATTAAAAACT TTTGCCTATC AAAAAAAAAA

3601 AAAAAAAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 176 bp to 2074 bp; peptide length: 633 Category: similarity to known protein

1 MPLTPTVQGF QWTLRGPDVE TSPFGAPRAA SHGVGRHQEL RDPTVPGPTS 51 SATNVSMVVS AGPWSGEKAE MNILEINKKS RPQLAENKQQ FRNLKQKCLV 101 TQVAYFLANR QNNYDYEDCK DLIKSMLRDE RLLTEEKLAE ELGQAEELRQ 151 YKVLVHSQER ELTQLREKLQ EGRDASRSLN QHLQALLTPD EPDNSQGRDL 201 REQLAEGCRL AQHLVQKLSP ENDDDEDEDV KVEEAEKVQE LYAPREVQKA 251 EEKEVPEDSL EECAITCSNS HHPCESNQPY GNTRITFEED QVDSTLIDSS 301 SHDEWLDAVC IIPENESDHE QEEEKGPVSP RNLQESEEEE APQESWDEGD 351 WTLSIPPDMS ASYQSDRSTF HSVEEQQVGL ALDIGRHWCD QVKKEDQEAT 401 SPRLSRELLD EKEPEVLQDS LDRFYSTPFE YLELPDLCQP YRSDFYSLQE 451 QHLGLALDLD RMKKDQEEEE DQGPPCPRLS RELPEVVEPE DLQDSLDRWY 501 STPFSYPELP DSCQPYGSCF YSLEEEHVGF SLDVDEIEKY QEGEEDQKPP 551 CPRLNEVLME AEEPEVLQDS LDRCYSTTST YFQLHASFQQ YRSAFYSFEE 601 QDVSLALDVD NRFFTLTVIR HHLAFQMGVI FPH

BLASTP hits

No BLASTP h ts available

Alert BLASTP hits for DKFZphtes3_7dl7, frame 2

PIR:T00069 hypothetical protein KIAA0454 - human (fragment), N = 1, Score = 199, P = le-11

PIR:A45592 liver stage antigen LSA-1 - Plasmodium falciparum, N = 1, Score = 158, P = 2 . le-01

>PIR:T00069 hypothetical protein KIAA0454 - human (fragment) Length = 1,882

HSPs:

Score = 199 (29.9 bits), Expect = l.Oe-11, P = l.Oe-11 Identities = 74/261 (28%), Positives = 122/261 (46%)

Query: 117 EDCKDLIKSMLRDERLLT EEKLAEELGQAEELRQYKVLVHSQERELTQLREKLQEG 172

+D + LI+ + + E L EEKLAEEL A +Y L+ Q REL+ LR+K++EG Sbjct: 964 KDLESLIQRVSQLEAQLPKNGLEEKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREG 1023 Query. 173 RDASRSLNQH LQALLTPDEPDNSQGRDLREQLAEGCRLAQHLVQKLSPENDDD 225

R + +H + LL ++ D G+ REQLA+G +L + L KLS ++ Sb_jct: 1024 RGICYLITRHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKS 1083

Query: 226 EDEDVKVEEAEKVQELYAPREVQKAEEK-EVPEDSLEECAITCSNSHHPCESNQPYGNTR 284

E + +E L RE+Q+ E+ EV + L+ ++T S+SH +S++ +T Sbjct: 1084 EKDQAGLEPLA LRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSTS 1139

Query: 285 ITFEEDQV—DSTLIDSSΞHDEWLDAVCIIPENESDHEQEEEKGPVSPRNLQESEEEEAP 342

+E + D ++ +H E A P + +S + S + A

Sbjct: 1140 FLSDELEACSDMDIVSEYTHYEEKKAS PSHΞDSIHHSSHSAVLSSKPSSTSASQGAK 1196

Query: 343 QESWDEGDWTLSIPPDMSASYQSDRSTFH 371

ES + +L P + S FH

Sbjct: 1197 AES-NSNPISLPTPQNTPKEANQAHSGFH 1224

Score = 89 (13.4 bits), Expect = 1. le-01, P = l.Oe-01 Identities = 35/89 (39%), Positives = 44/89 (49%)

Query: 464 KDQEEEEDQG PPCPRLSRELPEVVEP-EDLQDSLDRWYSTPFSYPELPDSCQ-PYGS 518

KD + E+DQ P RLSREL E + E LQ LD TP S L DS + P + Sbjct: 1079 KDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSST 1138

Query: 519 CFYSLEEEHVGFSLDVDEIEKYQEGEEDQKPP 550

F S E E D+D + +Y EE + P Sbjct: 1139 SFLSDELEACS DMDIVΞEYTHYEEKKASP 1167

Score = 73 (11.0 bits), Expect = 4.8e+00, P = 9.9e-01 Identities = 31/88 (35%), Positives = 40/88 (45%)

Query: 390 DQVKKEDQEATSP RLSRELLD-EKEPEVLQDSLDRFYSTPFEYLELPDLCQ-PYRSD 444

D ++DQ P RLSREL + EK EVLQ LD TP L D + P + Sbjct: 1080 DHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRΞPSSTS 1139

Query: 445 FYSLQEQHLGLALDLDRMKKDQEEEEDQGPP 475

F S L D+D + + EE + P Sbjct: 1140 FLS DELEACSDMDIVSEYTHYEEKKASP 1167

Score = 68 (10.2 bits), Expect = 1. le-01, P = 1.0e-01 Identities = 36/156 (23%), Positives = 68/156 (43%)

Query: 31 SHGVGRHQELRDPTV PGPTSSATNVSMVVΞAGPWS GEKAEMNILEINKK 79

S G +HQE + TV P P S + V A G ++ ++ +

Sbjct: 684 SPGKHQHQEEGNVTVRPFPRPQSLDLGATFTVDAHQLDNQSQPRDPGPQSAFSLPGSTQH 743

Query: 80 SRPQLAENKQQFRNLKQKCLVTQVAYFL-ANRQNNYDYE-DCKDLIKSMLRDERLLTEEK 137

R QL++ KQ++++L++K L+++ F AN Y + L+K + ++ ++ Sbjct: 744 LRSQLSQCKQRYQDLQEKLLLSEATVFAQANELEKYRVMLTGESLVKQDSKQIQVDLQDL 803

Query: 138 LAEELGQAEELRQYKVLVHSQERELTQLREK-LQEG 172

E G++E + + + E L+E L EG Sbjct: 804 GYETCGRSENEAEREETTSPECEEHNSLKEMVLMEG 839

Score = 65 (9.8 bits), Expect = 2.2e-01, P = ₂.0e-01 Identities = 23/96 (23%), Positives = 52/96 (54%)

Query: 123 IKSMLRDERLLTEEKLAEELGQAEE LRQYKVLVHSQERELTQLREKLQEGRDASRS 178

++ + D+ + E + E+ EE LRQ ++ V ++ +L +LR+ L ++ + Sbjct: 5 LRQRIHDKAVALERAIDEKFSALEEKEKELRQLRLAVRERDHDLERLRDVLS ΞNEA 60

Query: 179 LNQHLQALLTPDEPDNSQGRDLREQLAEGCRLAQHLVQKL 218

Q +++LL ++G ++ EQL+ C+ Q L +++ Sbjct: 61 TMQSMESLL RAKGLEV-EQLSTTCQNLQWLKEEM 93

Score = 61 (9.2 bits), Expect = 5.5e-01, P = 4.2e-01 Identities = 27/95 (28%), Positives = 47/95 (49%)

Query: 134 TEEK-LAEELGQAEELRQY KVLVHSQERELTQLREKLQEGRDASRSLNQHLQALLT 188

+E K L +LG+ EE R Y +LV +++ L+ +LQ ++L +++L Sbjct: 855 SERKPLENQLGKQEEFRVYGKΞENILV—LRKDIKDLKAQLQNANKVIQNLKSRVRSLSV 912

Query: 189 PDEPDNSQGRDLREQLAEGCRLAQHLVQKLSPENDDDEDE 228

+ +S R R+ A G ++ SP + DEDE Sbjct: 913 TSDYSSSLERP-RKLRAVGT LEGSSPHSVPDEDE 945

Score = 57 (8.6 bits), Expect = 1.4e+00, P = 7.5e-01 Identities = 26/92 (28%), Positives = 47/92 (51%)

Query: 127 LRDERLLTEEKLAEELGQAEEL RQYKVLVHSQERELTQLREKLQEGRDASRSLNQHL 183

L E LL EK+A Q +E+ R+ ++L+ + L R +L E A R L L Sbjct: 358 LTQEVLLLREKVASVESQGQEISGNRRQQLLLMLEG—LVDERSRLNEALQAERQLYSSL 415 Query: 184 QALLTPDEPDNSQ-GRDLREQLAEGCRLAQHLVQKL 218

P++S+ R L+ +L EG ++ + ++++ Sb_jct: 416 VKFHA—HPESSERDRTLQVEL-EGAQVLRSRLEEV 448

Score = 54 (8.1 bits), Expect = 2.7e+00, P = 9.3e-01 Identities = 61/264 (23%), Positives = 121/264 (45%)

Query: 3 LTPTVQGFQWTLRGPDVETSPFGAPRAASHGVGRHQE—LRDPTVPGPTSSATNVSMVVS 60

L+ T Q QW L+ ++ET F + + + + L D SAT ++ Sb_jct: 79 LSTTCQNLQW-LK-EEMETK-FSRWQKEQESIIQQLQTSLHDRNKEVEDLSAT LLCK 132

Query: 61 AGPWSGEKAEMNILEINKKSR PQLAENKQQFRNLKQKCLVTQVAYFLANRQNNYDYE 117

GP E AE + +K R L++ +Q L+ + + + ++ R+ Sbjct: 133 LGPGQSEIAEELCQRLQRKERMLQDLLSDRNKQV—LEHEMEIQGLLQSVSTREQE-SQA 189

Query: 118 DCKDLIKSMLRDERLLTEEKLAEELGQAEELRQYKVLVHSQERELT QLREKLQEG-- 172

+ L+++++ ER + L + LG + L + + +Q+ E+T +L ++ +G Sbjct: 190 AAEKLVQALM--ERNSELQALRQYLGGRDSLMS-QAPISNQQAEVTPTGRLGKQTDQGSM 246

Query: 173 RDASRSLNQHLQALLTPDEPDNSQGRDLREQLAEGCRLAQHLVQKLSPENDDDEDEDVKV 232

+ SR + L A P ++ G DL + +A G L ++LS N +E E + Sbjct: 247 QIPSRDDSTSLTAKEDVSIPRSTLG-DL-DTVA-G LEKELS—NAKEELELMAK 295

Query: 233 EEAEKVQELYAPREVQKAEEKEVPEDSLEECAIT 266

+E E EL A + + +E+E+ + + ++T Sbjct: 296 KERESQMELSALQSMMAVQEEELQVQAADMESLT 329

Score = 49 (7.4 bits). Expect = 6.3e+00, P = l.Oe+00 Identities = 21/87 (24%), Positives = 39/87 (44%)

Query: 192 PDNSQGRDLREQLAEGCRLAQHLVQKLSPENDDDEDEDVKVEEAEKVQELYAPREVQKAE 251

P ++Q LR QL++ + Q L +KL + + E EK + + + K + Sbjct: 738 PGSTQ—HLRSQLSQCKQRYQDLQEKLLLS EATVFAQANELEKYRVMLTGESLVKQD 792

Query: 252 EKEVPEDSLEECAI-TCSNSHHPCESNQ 278

K++ D L++ TC S + E + Sbjct: 793 SKQIQVD-LQDLGYETCGRSENEAEREE 819

Score = 46 (6.9 bits), Expect = 6.3e+00, P = 1.0e+00 Identities = 19/77 (24%), Positives = 39/77 (50%)

Query: 112 NNYDYEDCKDLIKSMLRDERLLTEEKLAEELGQAEELRQYKVLVHSQERELTQLREKLQ- 170

+ ++ E+ K+ K + E ++T+E L+E QAE R+ + + + + L+E+L Sbjct: 597 DGWEIEEDKE—KGEVMVETVVTKEGLSESSLQAE-FRKLQGKLKNAHNIINLLKEQLVL 653

Query: 171 EGRDASRSLNQHLQALLT 188

++ + L L LT Sbjct: 654 SSKEGNSKLTPELLVHLT 671

Pedant information for DKFZphtes3_7dl7, frame 2

Report for DKFZphtes3_7dl7.2

[LENGTH] 633

[MW] 72951.15

[pi] 4.40

[HOMOL] PIR:T00069 hypothetical protein KIAA0454 - human (fragment) 2e-ll

[BLOCKS] BL00201E

[PROSITE] MYRISTYL 2

[PROSITE] CK2_PHOSPHO_SITE 14

[PROSITE] PKC_PHOSPHO_SITE 4

[PROSITE] ASN_GLYCOSYLATION 2

[PFAM] TNFR/NGFR cysteine-rich region

[KW] All_Alpha

[KW] LOW_COMPLEXITY 4.90 %

[KW] COILED_COIL 6.95 %

SEQ MPLTPTVQGFQWTLRGPDVETSPFGAPRAASHGVGRHQELRDPTVPGPTSSATNVSMVVS

SEG

PRD ccccceeeeeeeecccccccccccccccccccccccccccccccccccccceeeeeeeee

COILS

SEQ AGPWSGEKAEMNILEINKKSRPQLAENKQQFRNLKQKCLVTQVAYFLANRQNNYDYEDCK

SEG

PRD ccccccchhhhhhhheeecccchhhhhhhhhhhcccccchhhhhhhhhhcccccccccch

COILS SEQ DLIKSMLRDERLLTEEKLAEELGQAEELRQYKVLVHSQERELTQLREKLQEGRDASRSLN SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccchhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ QHLQALLTPDEPDNSQGRDLREQLAEGCRLAQHLVQKLSPENDDDEDEDVKVEEAEKVQE SEG xxxxxxxxxxxxxxxx .. PRD hhhhhhhhccccccccchhhhhhhhhhhhhhhhhhhhhcccccccccchhhhhhhhhhhh COILS CCCCCCC

SEQ LYAPREVQKAEEKEVPEDSLEECAITCSNSHHPCESNQPYGNTRITFEEDQVDSTLIDSS SEG PRD hhhcchhhhhhhhhhcchhhhhhhccccccccccccccccccceeeeecccccccccccc COILS

SEQ SHDEWLDAVCIIPENEΞDHEQEEEKGPVSPRNLQESEEEEAPQESWDEGDWTLSIPPDMS SEG xxxxxxxxxxxxxxx PRD ccchhhhheeeccccccchhhhhhcccccccccchhhhhhhccccccccccccccccccc COILS

SEQ ASYQSDRSTFHSVEEQQVGLALDIGRHWCDQVKKEDQEATSPRLSRELLDEKEPEVLQDS SEG PRD ccccccccchhhhhhhhhhhhhhccccccchhhhhccccccchhhhhhhhhhhheeeecc COILS

SEQ LDRFYSTPFEYLELPDLCQPYRSDFYSLQEQHLGLALDLDRMKKDQEEEEDQGPPCPRLS SEG PRD hhhhhccceeeeecccccccccccchhhhhhhhhhhhhcchhhhhhhhhhcccccccccc COILS

SEQ RELPEVVEPEDLQDSLDRWYSTPFSYPELPDΞCQPYGSCFYSLEEEHVGFSLDVDEIEKY SEG PRD ccceeeeeccchhhhhhhhhccccccccccccccccccceeeeccceeeccccchhhhhh COILS

SEQ QEGEEDQKPPCPRLNEVLMEAEEPEVLQDSLDRCYSTTSTYFQLHASFQQYRSAFYΞFEE SEG PRD hcccccccccccchhhhhhhhhchhhhhccccceeecceeeehhhhhhhhhhhhhhhhhc COILS

SEQ QDVSLALDVDNRFFTLTVIRHHLAFQMGVIFPH SEG PRD cchhhhhhcccchhhhhhhhhhhhhhhhhcccc COILS

Prosite for DKFZphtes3_7dl7.2

PS00001 54->58 ASN_GLYCOSYLATION PDOC00001

PS00001 315->319 ASN_GLYCOSYLATION PDOC00001

PS00005 13->16 PKC_PHOSPHO_SITE PDOC00005

PS00005 329->332 PKC_PHOSPHO_SITE PDOC00005

PS00005 365->368 PKC_PHOSPHO_SITE PDOC00005

PS00005 401->4O4 PKC_PHOSPHO_SITE PDOC00005

PS00006 188->192 CK2_PHOSPHO_SITE PDOC00006

PS00006 259->263 CK2_PHOSPHO_SITE PDOC00006

PS00006 286->290 CK2_PHOSPHO_SITE PDOC00006

PS00006 295->299 CK2_PHOSPHO_SITE PDOC00006

PS00006 300->304 CK2_PHOSPHO_SITE PDOC00006

PS00006 317->321 CK2_PHOSPHO_SITE PDOC00006

PS00006 336->340 CK2_PHOSPHO_SITE PDOC00006

PS00006 345->349 CK2_PHOSPHO_SITE PDOC00006

PS00006 372->376 CK2_PHOSPHO_SITE PDOC00006

PS00006 427->431 CK2_PHOSPHO_SITE PDOC00006

PS00006 447->451 CK2_PHOΞPHO_SITE PDOC00006

PS00006 505->509 CK2_PHOSPHO_SITE PDOC00006

PS00006 522->526 CK2_PHOSPHO_SITE PDOC00006

PS00006 597->601 CK2_PHOSPHO_SITE PDOC00006

PS00008 25->31 MYRISTYL PDOC00008

PS00008 207->213 MYRISTYL PDOC00008

Pfam for DKFZphtes3_7dl7.2

HMM_NAME TNFR/NGFR cysteine-rich region

HMM *CpeGtYtDWNHvpqClpCtrCePEMGQYMvqPCTwTQNTVC*

C+ ++ + N+ ++ + ++ + +++ +++ ++VC Query 274 CESNQPYG-NT-RITFEEDQVDS— LIDSSSHDEWLDAVC 310 DKFZphtes3_7j3

group: cell cycle

DKFZphtes3_7j3.2 encodes a novel 628 ammo acid putative protein kinase, which is related to the C-TAKl Cdc25C associated protein kinase.

Cdc25C is a protein kinase that controls entry into mitosis by dephosphorylation of Cdc2. Cdc25C function is regulated by phosphorylation, too. Serine 216 phosphorylation of Cdc25C mediates the binding of 14-3-3 protein to Cdc₂5C. C-TAKl (Cdc twenty-five C associated protein kinase) phosphorylates Cdc25C on serine 216 in vitro. The new protein is closely related to C- Takl and therefore should be involved in cell-cycle regulation, too.

The new protein can find application in modulating/blocking the cell cycle. strong similarity to serine/threonme-specific protein kinases complete cDNA, complete eds, potential start at Bp 128, few EST hits

Sequenced by BMFZ

Locus : unknown

Insert length: 3443 bp

Poly A stretch at pos. 3399, polyadenylation signal at pos. 3376

1 GTGCTTTACT GCGCGCTCTG GTACTGCTGT GGCTCCCCGT CCTGGTGCGG 51 GACCTGTGCC CCGCGCTTCA GCCCTCCCCG CACAGCCTAC TGATTCCCCT

101 GCCGCCCTTG CTCACCTCCT GCTCGCCATG GAGTCGCTGG TTTTCGCGCG

151 GCGCTCCGGC CCCACTCCCT CGGCCGCAGA GCTAGCCCGG CCGCTGGCGG

201 AAGGGCTGAT CAAGTCGCCC AAGCCCCTAA TGAAGAAGCA GGCGGTGAAG

251 CGGCACCACC ACAAGCACAA CCTGCGGCAC CGCTACGAGT TCCTGGAGAC

301 CCTGGGCAAA GGCACCTACG GGAAGGTGAA GAAGGCGCGG GAGAGCTCGG

351 GGCGCCTGGT GGCCATCAAG TCAATCCGGA AGGACAAAAT CAAAGATGAG

401 CAAGATCTGA TGCACATACG GAGGGAGATT GAGATCATGT CATCACTCAA

451 CCACCCTCAC ATCATTGCCA TCCATGAAGT GTTTGAGAAC AGCAGCAAGA

501 TCGTGATCGT CATGGAGTAT GCCAGCCGGG GCGACCTTTA TGACTACATC

551 AGCGAGCGGC AGCAGCTCAG TGAGCGCGAA GCTAGGCATT TCTTCCGGCA

601 GATCGTCTCT GCCGTGCACT ATTGCCATCA GAACAGAGTT GTCCACCGAG

651 ATCTCAAGCT GGAGAACATC CTCTTGGATG CCAATGGGAA TATCAAGATT

701 GCTGACTTCG GCCTCTCCAA CCTCTACCAT CAAGGCAAGT TCCTGCAGAC

751 ATTCTGTGGG AGCCCCCTCT ATGCCTCGCC AGAGATTGTC AATGGGAAGC

801 CCTACACAGG CCCAGAGGTG GACAGCTGGT CCCTGGGTGT TCTCCTCTAC

851 ATCCTGGTGC ATGGCACCAT GCCCTTTGAT GGGCATGACC ATAAGATCCT

901 AGTGAAACAG ATCAGCAACG GGGCCTACCG GGAGCCACCT AAACCCTCTG

951 ATGCCTGTGG CCTGATCCGG TGGCTGTTGA TGGTGAACCC CACCCGCCGG 1001 GCCACCCTGG AGGATGTGGC CAGTCACTGG TGGGTCAACT GGGGCTACGC 1051 CACCCGAGTG GGAGAGCAGG AGGCTCCGCA TGAGGGTGGG CACCCTGGCA 1101 GTGACTCTGC CCGCGCCTCC ATGGCTGACT GGCTCCGGCG TTCCTCCCGC 1151 CCCCTCCTGG AGAATGGGGC CAAGGTGTGC AGCTTCTTCA AGCAGCATGC 1201 ACCTGGTGGG GGAAGCACCA CCCCTGGCCT GGAGCGCCAG CATTCGCTCA 1251 AGAAGTCCCG CAAGGAGAAT GACATGGCCC AGTCTCTCCA CAGTGACACG 1301 GCTGATGACA CTGCCCATCG CCCTGGCAAG AGCAACCTCA AGCTGCCAAA 1351 GGGCATTCTC AAGAAGAAGG TGTCAGCCTC TGCAGAAGGG GTACAGGAGG 1401 ACCCTCCGGA GCTCAGCCCA ATCCCTGCGA GCCCAGGGCA GGCTGCCCCG 1451 CTGCTCCCCA AGAAGGGCAT TCTCAAGAAG CCCCGACAGC GCGAGTCTGG 1501 CTACTACTCC TCTCCCGAGC CCAGTGAATC TGGGGAGCTC TTGGACGCAG 1551 GCGACGTGTT TGTGAGTGGG GATCCCAAGG AGCAGAAGCC TCCGCAAGCT 1601 TCAGGGCTGC TCCTCCATCG CAAAGGCATC CTCAAACTCA ATGGCAAGTT 1651 CTCCCAGACA GCCTTGGAGC TCGCGGCCCC CACCACCTTC GGCTCCCTGG 1701 ATGAACTCGC CCCACCTCGC CCCCTGGCCC GGGCCAGCCG ACCCTCAGGG 1751 GCTGTGAGCG AGGACAGCAT CCTGTCCTCT GAGTCCTTTG ACCAGCTGGA 1801 CTTGCCTGAA CGGCTCCCAG AGCCCCCACT GCGGGGCTGT GTGTCTGTGG 1851 ACAACCTCAC GGGGCTTGAG GAGCCCCCCT CAGAGGGCCC TGGAAGCTGC 1901 CTGAGGCGCT GGCGGCAGGA TCCTTTGGGG GACAGCTGCT TTTCCCTGAC 1951 AGACTGCCAG GAGGTGACAG CGACCTACCG ACAGGCACTG AGGGTCTGCT 2001 CAAAGCTCAC CTGAGTGGAG TAGGCATTGC CCCAGCCCGG TCAGGCTCTC 2051 AGATGCAGCT GGTTGCACCC CGAGGGGAGA TGCCTTCTCC CCCACCTCCC 2101 AGGACCTGCA TCCCAGCTCA GAAGGCTGAG AGGGTTTGCA GTGGAGCCCT 2151 GAGCAGGGCT GGATATGGGA AGTAGGCAAA TGAAATGCGC CAAGGGTTCA 2201 GTGTCTGTCT TCAGCCCTGC TGAACGAAGA GGATACTAAA GAGAGGGGAA 2251 CGGGAATGCC CGCGACAGAG TCCACATTGC CTGTTTCTTG TGTACATGGG 2301 GGGGCCACAG AGACCTGGAA AGAGAACTCT CCCAGGGCCC ATCTCCTGCA 2351 TCCCATGAAT ACTCTGTACA CATGGTGCCT TCTAAGGACA GCTCCTTCCC 2401 TACTCATTCC CTGCCCAAGT GGGGCCAGAC CTCTTTACAC ACACATTCCC 2451 GTTCCTACCA ACCACCAGAA CTGGATGGTG GCACCCCTAA TGTGCATGAG 2501 GCATCCTGGG AATGGTCTGG AGTAACGCTT CGTTATTTTT ATTTTTATTT 2551 TTATTTATTT ATTTATTTTT TTGAGACGGA GTTTCGCTCT TGGTGCCCAG

2601 GCTAGAGTGC AATGGCGCGA TCTCAGCTCA CCTCAACCTC CGCCTCCCGG

2651 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC TAGTAGCTGG GATTACAGGC

2701 GCCCGCCACC ATGCCCGGCT AATTTTGTAT TTTTAGTAGA GACAGGGTTT

2751 CTCCATGTTG GTCAGGCTGG TCTCAAACTC CCGACCTCAG GTGATCCACC

2801 CACCTCGGCC TCCCAAAGTG CTGGGATTAC AGGCGTGAGC CACCGCGCCC

2851 CACCTAACCC TTCCTTATTT AGCCTAGGAG TAAGAGAACA CAATCTCTGT

2901 TTCTTCAATG GTTCTCTTCC CTTTTCCATC CTCCAAACCT GGCCTGAGCC

2951 TCCTGAAGTT GCTGCTGTGA ATCTGAAAGA CTTGAAAAGC CTCCGCCTGC

3001 TGTGTGGACT TCATCTCAAG GGGCCCAGCC TCCTCTGGAC TCCACCTTGG

3051 ACCTCAGTGA CTCAGAACTT CTGCCTCTAA GCTGCTCTAA AGTCCAGACT

3101 ATGGATGTGT TCTCTAGGCC TTCAGGACTC TAGAATGTCC ATATTTATTT

3151 TTATGTTCTT GGCTTTGTGT TTTAGGAAAA GTGAATCTTG CTGTTTTCAA

3201 TAATGTGAAT GCTATGTTCT GGGAAAATCC ACTATGACAT CTAAGTTTTG

3251 TGTACAGAGA GATATTTTTG CAACTATTTC CACCTCCTCC CACAACCCCC

3301 CACACTCCAC TCCACACTCT TGAGTCTCTT TACCTAATGG TCTCTACCTA

3351 ATGGACCTCC GTGGCCAAAA AGTACCATTA AAACCAGAAA GGTGATTGGA

3401 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAA

BLAST Results

No BLAST result

Medline entries

98202387:

C-TAKl protein kinase phosphorylates human Cdc25C on serine 216 and promotes 14-3-3 protein binding.

Peptide information for frame 2

ORF from 128 bp to 2011 bp; peptide length: 628 Category: strong similarity to known protein

1 MESLVFARRS GPTPSAAELA RPLAEGLIKS PKPLMKKQAV KRHHHKHNLR

51 HRYEFLETLG KGTYGKVKKA RESSGRLVAI KSIRKDKIKD EQDLMHIRRE

101 IEIMSSLNHP HIIAIHEVFE NSSKIVIVME YASRGDLYDY ISERQQLSER

151 EARHFFRQIV SAVHYCHQNR VVHRDLKLEN ILLDANGNIK IADFGLSNLY

201 HQGKFLQTFC GSPLYASPEI VNGKPYTGPE VDSWSLGVLL YILVHGTMPF

251 DGHDHKILVK QISNGAYREP PKPSDACGLI RWLLMVNPTR RATLEDVASH

301 WWVNWGYATR VGEQEAPHEG GHPGSDSARA SMADWLRRSS RPLLENGAKV

351 CSFFKQHAPG GGSTTPGLER QHSLKKSRKE NDMAQSLHSD TADDTAHRPG

401 KSNLKLPKGI LKKKVSASAE GVQEDPPELS PIPASPGQAA PLLPKKGILK

451 KPRQRESGYY SSPEPSESGE LLDAGDVFVS GDPKEQKPPQ ASGLLLHRKG

501 ILKLNGKFSQ TALELAAPTT FGSLDELAPP RPLARASRPS GAVSEDSILS

551 SEΞFDQLDLP ERLPEPPLRG CVSVDNLTGL EEPPSEGPGS CLRRWRQDPL

601 GDSCFSLTDC QEVTATYRQA LRVCSKLT

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_7j3, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphtes3_7j3, frame 2

Report for DKFZphtes3_7j3.2

[LENGTH] 628

[MW] 69612.39

[pi] 9.01

[HOMOL] TREMBL:AB011109_1 gene: "KIAA0537"; product: "KIAA0537 protein"; Homo sapiens mRNA for KIAA0537 protein, complete eds. le-152

[FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YDR477w] 5e-66

[FUNCAT] 11.01 stress response [S. cerevisiae, YDR477w] 5e-66 FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDR477w] 5e-66

FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YLR096w] 6e-54

FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YLR096w] 6e-54

FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YDR507c] e-52

FUNCAT] 03.25 cytokinesis [S. cerevisiae, YDR507c] 8e-52

FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YKLlOlw] 9e-51

FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKLlOlw] 9e-51

FUNCAT] 99 unclassified proteins [S. cerevisiae, YPL141c] le-45

FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YPL153c] 6e-44

FUNCAT] 03.22.01 cell cycle check point proteins [S. cerevisiae, YPL153c] 6e-44

FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YPL153c] 6e-44

FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YPL153c] 6e-44

FUNCAT] 03.16 dna synthesis and replication [Ξ. cerevisiae, YMROOlc] 2e-42

FUNCAT] 10.02.11 key kinases [S. cerevisiae, YBL105c] 3e-34

FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YKL139w CTK1 - carboxy- ermmal domain] 2e-28

FUNCAT] 03 01 cell growth [S. cerevisiae, YFR014c] 4e-28 FUNCAT] 03 10 sporulation and germination [S. cerevisiae, YGL180w] 2e-26 FUNCAT] 06 13.04 lysosomal and vacuolar degradation [S. cerevisiae, YGL180w] 2e-26 FUNCAT] 08 13 vacuolar transport [S. cerevisiae, YGL180w] 2e-26 FUNCAT] 04 99 other transcription activities [S. cerevisiae, YER129w] 4e-26 FUNCAT] 02 19 metabolism of energy reserves (glycogen, trehalose) [S. cerevisiae, YPL031C] 5e-24 FUNCAT] 01 04.04 regulation of phosphate utilization [S. cerevisiae, YPL031c] e-24 FUNCAT ] 03.07 pheromone response mating-type determination, sex-specific proteins

[S. cerevisiae, YHL007c] 6e-24

FUNCAT] 10.05.11 key kinases [S. cerevisiae, YHL007c] 6e-24

FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YNR031C] le-22

FUNCAT] 10.03.11 key kinases [S. cerevisiae, YNR031c] le-22

FUNCAT] 03.13 meiosis [S. cerevisiae, YDR523c] 8e-22

FUNCAT] 04.05.01.01 general transcription activities [S. cerevisiae, YDL108w] e-21

FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation farnesylation and processing) [S. cerevisiae, YFL033c] 6e-21

FUNCAT] 10.05.09 regulation of g-protein activity [S. cerevisiae, YBL016w] 7e-19

FUNCAT] 10.04.11 key kinases [Ξ. cerevisiae, YDL159w] 3e-18

FUNCAT] 01.02.04 regulation of nitrogen and sulphur utilization [S. cerevisiae, YNL183C] le-17

FUNCAT] 08.99 other mtracellular-transport activities [S. cerevisiae, YNL183c] e-17

FUNCAT] 05, 07 translational control [S. cerevisiae, YDR283c] 2e-17

FUNCAT] 09, 04 biogenesis of cytoskeleton [S. cerevisiae, YNL020c] 4e-16

FUNCAT] 04.03.99 other trna-transcription activities [S. cerevisiae, YOR061w] e-15

FUNCAT] 10.04.99 other nutritional-response activities [S. cerevisiae, YJR059w] 5e-15

FUNCAT] c energy conversion [M. gemtalium, MG109] 3e-12

FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YBR097W] 2e-08

FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YBR097w] e-08

FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YBR097w] e-08

FUNCAT] 30. organization of golgi [S. cerevisiae, YBR097w] 2e-08

FUNCAT] 30. organization of endoplasmatic reticulum [S. cerevisiae, YHR079c] e-05

FUNCAT] 01.06.10 regulation of lipid, fatty-acid and sterol biosynthesis [S. cerevisiae, YHR079C] 8e-05

BLOCKS] BL00479C Phorbol esters / diacylglycerol binding domain proteins

BLOCKS] BL00239B Receptor tyrosine kinase class II proteins

BLOCKS] BL00107A Protein kinases ATP-binding region proteins

SCOP] dlgol 5.1.1.1.9 MAP kinase Erk2 [rat Rattus norvegicus le-77

SCOP] dlwfc 5.1.1.1.8 MAP kinase p38 [human (Homo sapiens) 4e-68

SCOP] dlkoa_2 5.1.1.1.7 (1-350) Twitchm, kinase domain [Caenorhabditi 2e-85 SCOP] dlkoba_ 5.1.1.1.6 Twitchm, kinase domain [California sea har le-80

SCOP] dlphk 5.1.1.1.5 gamma-subunit of glycogen phosphorylase kinas 2e-76

SCOP] dlirk 5.1.1.2.4 insulin receptor [Human (Homo sapiens) le-69

SCOP] dlapme_ 5.1.1.1.4 cAMP-dependent PK, catalytic subunit [mouse (Mu le-84 SCOP] dlfgka_ 5.1.1.2.3 Fibroblast growth factor receptor 1 [human (Hom le-68 SCOP] dlydre_ 5.1.1.1.3 cAMP-dependent PK, catalytic subunit [bovine (Bo 9e-85 SCOP] dlfmk_3 5.1.1.2.2 (168-437) c-src tyrosine kinase [human (Hom le-69 SCOP] dlcdka_ 5.1.1.1.2 cAMP-dependent PK, catalytic subunit [pig (Su le-85 SCOP] d2hcka3 5.1.1.2.1 (167-437) Haemopoetic cell kinase Hck [huma 5e-66

SCOP] dlcsn 5.1.1.1.11 Casein kιnase-1, CK1 [Schizosaccharomyces pombe 9e-47

SCOP] dljsua_ 5.1.1.1.1 Cyclin-dependent PK [Human (Homo sapiens) le-75 SCOP] dlckja_ 5.1.1.1.10 Casein kιnase-1, CK1 [rat (Rattus norvegicus) 5e-54

EC] 2.7.1.38 Phosphorylase kinase le-36

EC] 2.7.1.123 Ca2+/calmodulιn-dependent protein kinase 4e-40 [EC] 2.7.1.128 [Acetyl-CoA carboxylase] kinase le-61

[EC] 2.7.1.117 Myosin-light-chain kinase 2e-40

[EC] 2.7.1.109 [Hydroxymethylglutaryl-CoA reductase (NADPH) ] kinase le-61

[EC] 2.7.1.37 Protein kinase 7e-42

[PIRKW] phosphotransferase 6e-66

[PIRKW] nucleus le-64

[PIRKW] calcium 7e-35

[PIRKW] duplication le-38

[PIRKW] tandem repeat 4e-39

[PIRKW] phorbol ester binding le-38

[PIRKW] zinc le-38

[PIRKW] cell cycle control le-42

[PIRKW] serme/threonme-specific protein kinase 8e-68

[PIRKW] oncogene le-40

[PIRKW] phospholipid binding le-38

[PIRKW] autophosphorylation le-64

[PIRKW] brain le-40

[PIRKW] heterotetramer 2e-36

[PIRKW] mitosis 7e-42

[PIRKW] polymer le-35

[PIRKW] magnesium 6e-66

[PIRKW] ATP 8e-68

[PIRKW] polyprotein le-40

[PIRKW] phosphoprotem le-64

[PIRKW] apoptosis 4e-39

[PIRKW] glycoprotein 7e-42

[PIRKW] leucine zipper 3e-35

[PIRKW] skeletal muscle 7e-35

[PIRKW] protein kinase 5e-41

[PIRKW] cAMP binding 3e-38

[PIRKW] testis 9e-36

[PIRKW] purme nucleotide binding 2e-49

[PIRKW] calcium binding 8e-39

[PIRKW] alternative splicing 3e-37

[PIRKW] P-loop 2e-49

[PIRKW] lipoprotein 2e-33

[PIRKW] segmentation le-33

[PIRKW] core protein le-40

[PIRKW] muscle 7e-35

[PIRKW] myristylation 2e-33

[PIRKW] EF hand 8e-39

[PIRKW] cell division 2e-40

[PIRKW] calmodulin binding 4e-40

[SUPFAM] ribosomal protein S6 kinase II 5e-36

[SUPFAM] fibronectin type III repeat homology 3e-33

[SUPFAM] lmmunoglobulin homology 3e-33

[SUPFAM] calcium-dependent protein kinase 8e-39

[SUPFAM] AMP-activated protein kinase 6e-66

[SUPFAM] protein kinase akt 3e-42

[SUPFAM] protein kinase SPK1 le-42

[SUPFAM] unassigned Ser/Thr or Tyr-specific protein kinases 8e-68

[SUPFAM] Ca2+/calmodulιn-dependent protein kinase 3e-37

[SUPFAM] calmodulin repeat homology 8e-39

[SUPFAM] cAMP receptor protein cyclic nucleotide-binding domain homology 6e-33

[SUPFAM] protein kinase C zeta le-36

[SUPFAM] Dictyostelium cAMP-dependent protein kinase catalytic chain le-34

[SUPFAM] death-associated protein kinase 4e-39

[SUPFAM] pleckstrm repeat homology 3e-42

[SUPFAM] ankyrin repeat homology 4e-39

[SUPFAM] protein kinase homology 8e-68

[SUPFAM] Ca2+/calmodulm-dependent protein kinase II 8e-41

[SUPFAM] protein kinase C zmc-bmding repeat homology le-38

[SUPFAM] twitchm 3e-33

[SUPFAM] protein kinase C delta le-38

[SUPFAM] cGMP-dependent protein kinase 6e-33

[SUPFAM] protein kinase cdrl 7e-42

[SUPFAM] protein kinase C C2 region homology 3e-37

[SUPFAM] protein kinase C alpha 3e-37

[SUPFAM] yeast protein kinase C 5e-36

[SUPFAM] kinase-related transforming protein le-41

[SUPFAM] kinase interaction domain homology le-42

[SUPFAM] gag-akt polyprotein le-40

[SUPFAM] Ca2+/calmodulιn-dependent protein kinase I 4e-40

[SUPFAM] protein kinase C mu 4e-33

[PROSITE] PROTEIN_KINASE_ATP 2

[PROSITE] RGD 1

[PROSITE] MYRISTYL 4

[PROSITE] CAMP_PHOSPHO_SITE 3

[PROSITE] CK2_PHOSPHO_SITE 13

[PROSITE] TYR_PHOSPHO_SITE 2

[PROSITE] PKC PHOSPHO_SITE 12 [PROSITE] ASN_GLYCOSYLATION 2

[PROSITE] PROTEIN_KINASE_ST 1

[PFAM] Eukaryotic protein kinase domain

[KW] All_Alpha

[KW] 3D

[KW] LOW COMPLEXITY 10.51 %

SEQ MESLVFARRSGPTPSAAELARPLAEGLIKSPKPLMKKQAVKRHHHKHNLRHRYEFLETLG SEG xxxxxxxxxxxx IctpE HHHHHHHHHHHHHHHCCCCCCCC—GGGEEEEEEEE

SEQ KGTYGKVKKARESSGRLVAIKSIRKDKIKDEQDLMHIRREIEIMSSLNHPHIIAIHEVFE SEG IctpE CTTTEEEEEEEETTTEEEEEEEEEHHHHHHHCCHHHHHHHHHHHHCCCTTTBCCEEEEEE

SEQ NSSKIVIVMEYASRGDLYDYISERQQLΞEREARHFFRQIVSAVHYCHQNRVVHRDLKLEN SEG IctpE ETTEEEEEEECTTTTBHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEECCCCCGGG

SEQ ILLDANGNIKIADFGLSNLYHQGKFLQTFCGSPLYAΞPEIVNGKPYTGPEVDSWSLGVLL SEG IctpE EEETTTTCEEECCTTTTEET-TTT-BCCCCCCGGGCCHHHHHCCCBC-HHHHHHHHHHHH

SEQ YILVHGTMPFDGHDHKILVKQISNGAYREPPKPSDACGLIRWLLMVNPTRRATLEDVASH SEG IctpE HHHHHCCTTTTTTTHHHHHHHHHHCCCCCTTTCHHHHHHHHHTTTTTGGGTTTHHHHHHC

SEQ WWVNWGYATRVGEQEAPHEGGHPGSDSARASMADWLRRSSRPLLENGAKVCSFFKQHAPG SEG

IctpE GG

SEQ GGSTTPGLERQHSLKKSRKENDMAQSLHSDTADDTAHRPGKSNLKLPKGILKKKVSASAE SEG IctpE

SEQ GVQEDPPELSPIPASPGQAAPLLPKKGILKKPRQRESGYYSSPEPSESGELLDAGDVFVS SEG xxxxxxxxxxxx ... xxxxxxxxxxxxxxx IctpE

SEQ GDPKEQKPPQASGLLLHRKGILKLNGKFSQTALELAAPTTFGSLDELAPPRPLARASRPS SEG xxxxxxxxxxxxxx IctpE

SEQ GAVSEDSILSSESFDQLDLPERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPL SEG xxxxxxxxxxxxx IctpE

SEQ GDSCFSLTDCQEVTATYRQALRVCSKLT SEG IctpE

Prosite for DKFZphtes3_7j3.2

PS00001 121->125 ASN_GLYCOSYLATION PDOC00001 PS00001 576->580 ASN_GLYCOSYLATION PDOC00001 PS00004 290->294 CAMP_PHOSPHO_SITE PDOC00004 PS00004 337->341 CAMP_PHOSPHO_SITE PDOC00004 PS00004 413->417 CAMP_PHOSPHO_SITE PDOC00004 PS00005 30->33 PKC_PHOSPHO_SITE PDOC00005 PS00005 74->77 PKC_PHOSPHO_SITE PDOC00005 PS00005 82->85 PKC_PHOSPHO_SITE PDOC00005 PS00005 122->125 PKC_PHOSPHO_SITE PDOC00005 PS00005 142->145 PKC_PHOSPHO_SITE PDOC00005 PS00005 148->151 PKC_PHOSPHO_SITE PDOC00005 PS00005 289->292 PKC_PHOSPHO_SITE PDOC00005 PS00005 327->330 PKC_PHOSPHO_SITE PDOC00005 PS00005 339->342 PKC_PHOSPHO_SITE PDOC00005 PS00005 373->376 PKC_PHOSPHO_SITE PDOC00005 PS00005 377->380 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 616->619 PKC_PHOSPHO_SITE PDOC00005 PS00006 15->19 CK2_PHOSPHO_SITE PDOC00006 PS00006 133->137 CK2_PHOSPHO_SITE PDOC00006 PS00006 148->152 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 227->231 CK2_PHOSPHO_SITE PDOC00006 PS00006 293->297 CK2_PHOSPHO_SITE PDOC00006 PS00006 331->335 CK2_PHOSPHO_SITE PDOC00006 PS00006 377->381 CK2_PHOSPHO_SITE PDOC00006 PS00006 391->395 CK2 PHOSPHO SITE PDOC00006 PS00006 461->465 CK2_PHOSPHO_SITE PDOC00006

PS00006 511->515 CK2_PHOSPHO_SITE PDOC00006

PS00006 523->527 CK2_PHOSPHO_SITE PDOC00006

PS00006 578->582 CK2_PHOSPHO_SITE PDOC00006

PS00006 606->610 CK2_PHOSPHO_SITE PDOC00006

PS00007 453->460 TYR_PHOSPHO_SITE PDOC00007

PS00007 453->461 TYR_PHOSPHO_SITE PDOC00007

PS00008 320->326 MYRISTYL PDOC00008

PS00008 324->330 MYRISTYL PDOC00008

PS00008 347->353 MYRISTYL PDOC00008

PS00008 360->366 MYRISTYL PDOC00008

PS00016 134->137 RGD PDOC00016

PS00107 59->82 PROTEIN_KINASE_ATP PDOC00100

PS00107 59->86 PROTEIN_KINASE_ATP PDOC00100

PS00108 171->184 PROTEIN KINASE ST PDOC00100

Pfam for DKFZphtes3_7j3.2

HMM_NAME Eukaryotic protein kinase domain

HMM *YeιgRιIGeGsFGtVYkCιWrTGeIVAIKIIkkrsms F1REI

YE+++++G+G++G+V+K+++ +G++VAIK I+K++++ ++REI

Query 53 YEFLETLGKGTYGKVKKARESSGRLVAIKSIRKDKIKDEQDLMHIRREI 101

HMM qlMRrLnHPNIIRFYDwFedddDHIYMIMEYMeGGDLFDYIrrngpMsEw

+IM +LNHP+II + ++FE ++ I ++MEY+ GDL+DYI+++ ++SE+

Query 102 EIMSSLNHPHIIAIHEVFE-NSSKIVIVMEYAΞRGDLYDYISERQQLSER 150

HMM elrflMyQILrGMeYLHSMgllHRDLKPENILIDeNgqlKIcDFGLARqM

E+R++++QI++++ Y+H ++++HRDLK ENIL+D NG+IKI+DFGL+ ++

Query 151 EARHFFRQIVSAVHYCHQNRVVHRDLKLENILLDANGNIKIADFGLSNLY 200

HMM nnYerMttfCGTPWYMMAPEVIImg.nyYttkVDMWSFGCILWEMMTGep + + ++ TFCG+P Y +PE+ ++G +Y +++VD WS+G++L++++ G+

Query 201 HQGKFLQTFCGSPLYA-SPEI-VNGKPYTGPEVDSWSLGVLLYILVHGTM 248

HMM PFyddnMemlmrliqrfrrpfWpnCSeElyDFMrwCWnyDPekRPTFrQI

PF+++ ++ I + +++ +P S+ + ++RW++ ++P++R T +++

Query 249 PFDGHDHKILVKQISNGAYREPPKPSD-ACGLIRWLLMVNPTRRATLEDV 297

HMM LnHPWF* H W+

Query 298 ASHWWV 303

DKFZphtes3_7 8

group: testes derived

DKFZphtes3_7j8 encodes a novel 410 amino acid protein nearly identical to human WUGSC :H_DJ1159O04.1.

The novel protein contains an additional C-terminal domain, which is not present m

WUGSC :H_DJ1159O04.1.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes .

WUGSC :H_DJ1159O04.1 similarity to YBL104p verifies and extends the genmodel WUGSC : H_DJ1159O04.1 similarity to S. cerevisiae YBL104p

Sequenced by BMFZ

Locus: /map="7p21-p22"

Insert length: 3353 bp

Poly A stretch at pos. 3231, no polyadenylation signal found

1 GCAAAATATG TTGTATTTGT GGCATAGTTC ATATTTACAC TATCATAAAA

51 TTATGGCCGA GAAGTTAAAT ATTCTAAATG TGTCAACATA GTTCTCTGTA

101 AAACTGACTT ATTTTCCAAA TATATTTTGA AATAAAACAA TATAAAAATG

151 TTTTCTGTTT TTAGGAATGG TGGAAAGCAG CAGACATAAT TGGAGTGGGT

201 TGGATAAGCA AAGTGATATT CAAAATTTAA ATGAAGAGAG AATCTTAGCT

251 TTACAGCTTT GTGGGTGGAT AAAGAAAGGA ACGGATGTAG ACGTGGGGCC

301 ATTTTTGAAC TCCCTTGTAC AAGAAGGGGA ATGGGAAAGA GCTGCTGCTG

351 TGGCATTGTT CAACTTGGAT ATTCGCCGAG CAATCCAAAT CCTGAATGAA

401 GGGGCATCTT CTGAAAAAGG AGATCTGAAT CTCAATGTGG TAGCAATGGC

451 TTTATCGGGT TATACGGATG AGAAGAACTC CCTTTGGAGA GAAATGTGTA

501 GCACACTGCG ATTACAGCTA AATAACCCGT ATTTGTGTGT CATGTTTGCA

551 TTTCTGACAA GTGAAACAGG ATCTTACGAT GGAGTTTTGT ATGAAAACAA

601 AGTTGCAGTA CGTGACAGAG TGGCATTTGC TTGTAAATTC CTTAGTGATA

651 CTCAGTTAAA TAGATACATC GAAAAGTTGA CCAATGAAAT GAAAGAGGCT

701 GGAAATTTGG AAGGAATTTT GCTTACAGGC CTTACTAAAG ATGGAGTGGA

751 CTTAATGGAG AGTTATGTTG ATAGAACTGG AGATGTTCAA ACAGCAAGTT

801 ACTGTATGTT ACAGGGTTCA CCTTTAGATG TTCTTAAAGA TGAAAGGGTT

851 CAGTACTGGA TTGAGAATTA TAGAAATTTA TTAGATGCCT GGAGGTTTTG

901 GCATAAACGA GCTGAATTTG ATATTCACAG GAGTAAGTTG GATCCCAGTT

951 CCAAGCCTTT AGCACAAGTT TTTGTGAGTT GCAATTTCTG TGGCAAGTCA

1001 ATCTCCTACA GCTGTTCAGC TGTGCCTCAT CAGGGCAGAG GTTTTAGTCA

1051 GTATGGTGTG AGTGGCTCAC CAACGAAATC TAAAGTCACA AGTTGTCCTG

1101 GCTGTCGAAA ACCACTTCCT CGATGTGCGC TTTGTCTCAT TAATATGGGA

1151 ACACCAGTTT CTAGCTGTCC TGGAGGAACC AAATCAGATG AAAAAGTGGA

1201 CTTGAGCAAG GACAAAAAAT TAGCCCAATT TAACAACTGG TTTACATGGT

1251 GTCATAATTG CAGGCACGGT GGACATGCTG GACATATGCT TAGTTGGTTC

1301 AGGGACCATG CAGAGTGCCC TGTGTCTGCA TGCACGTGTA AATGTATGCA

1351 GTTGGATACA ACGGGGAATC TGGTACCTGC AGAGACTGTC CAGCCATAAA

1401 ATGTTACCAC CTTAAGAGAA CCCTTCAAGT GTGGAGCTTT CTAGTAGGTG

1451 TCCTTCATAG CTCAGAAACA TACCTCAGAA CAAGCCATTC ATGACTTACC

1501 TGTAATGGGA AAATAAATCA TTCTATCAGA TCAGCAGTTT TGATGTTTGA

1551 GTGATTTTGA TATGCTTCAC AGAGACAAAT GCTGCCAAAA TAAACATCGA

1601 AGTATAGACA TGAGTTCTGT TCAGCAGGTT GAAAAGTCTG ATTTAGAAAA

1651 ACTTTCTAAG TTTTGGTTGA AATTATGAAC ACTCTAGAAG CAGAATTTCT

1701 GGAAGAGCCA AGAACAGACT TTGAGCCTAT ATCTTCAAAG CTGAAACTGG

1751 ATATCTTTCA ATAAAATATG TGCACTTTTA AAATAAAATG ACTAATTCTG

1801 TGATTCAGAC AATAGTTTTA AGTTCAGCTG TGCTTAGATT TCTTTCAGAT

1851 TAATTTAAAA TTATAGATTT TTACTTTTAG AATTGCAGAG CCCCTATCCC

1901 ACACTGGAGA ATATTTTTTA TTACTGTCTG TTATATATGT GTCTATGTGT

1951 GTGTGTATAT TTATGTGTGT ATGTATAAAT ATGTACTTTT TAAAGGAGCC

2001 TTTTCCCTCC TTTGATTTTA AGATAAGCAA TCTTTTGGCA TAACATTATC

2051 GTCTTCCTAG AAAAGCCAAG ATGAAGAATC TATCTTACAA CTTTTTCTCT

2101 TCAGTAGAGA AAAACATGTA CCATTTCAGG TGAACATACA AAATTTTCAC

2151 TTTCTACCTT TTGCCTTCCA ATGTCCTGAT TTGTCTTCAA AGGTTTTTCT

2201 CCATATTAAT TTGTCATCTT ATCCTCATCA CCTGAGAACA TTTTACTGCA

2251 TACAAAGTCT ATGCAAGATT ATATGTAACT AGCCATTTAG TATAATCTAT

2301 GTCAGTGTTT CTGTGCTGTC AAATTCCGTC CTGATTTGGA ATACCATACC

2351 TTGTTCTTTC CAAGGTAGAC TAGGAAGTGT TGGGGAAATA GGGTCACTTC

2401 AGAGACCATT TTAGATGTAA GTTTTTAAAT GTAAGTGTTA CTGGGGCTAA

2451 GTCAGGGACT TTATTTAAAA CATTTTTTTT TTCTCATTTC ATAGCTAGAT

2501 AGTTGTAAGA GAAATACAAA GAATTTACAA GATGCTTCTC TGTCATCTGC 2551 CGTATGCAGA GGGACTGAAC TAGGAATTTT GTAGTTGAAG CTGTGTTCAT

2601 AAAGAGTAAA TCTTATTTTA TAGATTTTGG AGAAATAAAA CAAGAATTTT

2651 AAGAGCTTTC GTATTAGCAG TTTTGCCTTA TAAAAACTAA GATTTGTCAG

2701 ATTAGTTTGA GGTGTAACCT AAATATTAAA AGTAGATTAA ATTTATTTTT

2751 TACCTTGAGT GTCTGATACA TAAAACCCTT TTCTAGGAAA ACATTGGAAG

2801 TAGTACATAT TTACTCTAAA TGTCTCACCT GCATGACAGT CTTTTCAAAT

2851 GAAAGACATG GTAATTGCAA TTTTTTTTTA AAGATTGCTA TTAAGGGTAC

2901 TTTTTCCAGC CTTCATTTGA GTAAATCTTA ATTGATTTCA TTTTATTAAC

2951 ATATACCCTT TACCTTTAAT ATTTCATTTG AAGTGTTCCT TTCAAACTTA

3001 CTGTCTTAAA TATGAAAGTC AGCTTTAAGT AATGTCAGAC TCATATGCAT

3051 TTTCATTCTC ATTAGCTAAA GTAAAATGTA AAATTATCTC AAATAGTTAC

3101 AAGTTTTGGA AATACAGTAT AAAACATGAA TGTAAAGTCT ATTATGTAAT

3151 ATGCTTATTT GTAATCCTAA TATATGAGGG TGACATTTTT AAGATTGTAT

3201 GTATGTGTCA ACCTCTTAAA TGTTTTCTGT GAAAAAAAAA AAAAAAAAAA

3251 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

3301 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 3351 AAA

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 167 bp to 1396 bp; peptide length: 410 Category: known protein Classification: unclassified

1 MVESSRHNWS GLDKQSDIQN LNEERILALQ LCGWIKKGTD VDVGPFLNSL 51 VQEGEWERAA AVALFNLDIR RAIQILNEGA SSEKGDLNLN VVAMALSGYT 101 DEKNSLWREM CSTLRLQLNN PYLCVMFAFL TSETGSYDGV LYENKVAVRD 151 RVAFACKFLS DTQLNRYIEK LTNEMKEAGN LEGILLTGLT KDGVDLMESY 201 VDRTGDVQTA SYCMLQGSPL DVLKDERVQY WIENYRNLLD AWRFWHKRAE 251 FDIHRSKLDP SSKPLAQVFV SCNFCGKSIS YSCSAVPHQG RGFSQYGVSG 301 SPTKSKVTSC PGCRKPLPRC ALCLINMGTP VSSCPGGTKS DEKVDLSKDK 351 KLAQFNNWFT WCHNCRHGGH AGHMLSWFRD HAECPVSACT CKCMQLDTTG 401 NLVPAETVQP

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_7j8, frame 2

PIR:S45391 probable membrane protein YBL104c - yeast (Saccharomyces cerevisiae), N = 2, Score = 446, P = 4.5e-47

TREMBL:AC004982_1 gene: "WUGSC :H_DJ1159O04.1"; Homo sapiens PAC clone DJ1159O04 from 7p21-p22, complete sequence., N = 1, Score = 2038, P = 7.6e-211

>TREMBL:AC004982_1 gene: "WUGSC:H_DJ1159O04.1"; Homo sapiens PAC clone DJ1159O04 from 7p21-p22, complete sequence. Length = 379

HSPs:

Score = 2038 (305.8 bits), Expect = 7.6e-211, P = 7.6e-211 Identities = 379/379 (100%), Positives = 379/379 (100%)

Query: 1 MVESSRHNWSGLDKQSDIQNLNEERILALQLCGWIKKGTDVDVGPFLNSLVQEGEWERAA 60

MVESSRHNWSGLDKQSDIQNLNEERILALQLCGWIKKGTDVDVGPFLNSLVQEGEWERAA Sb_jct: 1 MVESSRHNWSGLDKQSDIQNLNEERILALQLCGWIKKGTDVDVGPFLNSLVQEGEWERAA 60

Query: 61 AVALFNLDIRRAIQILNEGASSEKGDLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNN 120

AVALFNLDIRRAIQILNEGASSEKGDLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNN Sb_jct: 61 AVALFNLDIRRAIQILNEGASSEKGDLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNN 120 Query: 121 PYLCVMFAFLTSETGSYDGVLYENKVAVRDRVAFACKFLSDTQLNRYIEKLTNEMKEAGN 180

PYLCVMFAFLTSETGSYDGVLYENKVAVRDRVAFACKFLSDTQLNRYIEKLTNEMKEAGN Sbjct: 121 PYLCVMFAFLTSETGSYDGVLYENKVAVRDRVAFACKFLSDTQLNRYIEKLTNEMKEAGN 180

Query: 181 LEGILLTGLTKDGVDLMESYVDRTGDVQTASYCMLQGSPLDVLKDERVQYWIENYRNLLD 240

LEGILLTGLTKDGVDLMESYVDRTGDVQTASYCMLQGSPLDVLKDERVQYWIENYRNLLD Sbjct: 181 LEGILLTGLTKDGVDLMESYVDRTGDVQTASYCMLQGSPLDVLKDERVQYWIENYRNLLD 240

Query: 241 AWRFWHKRAEFDIHRSKLDPSΞKPLAQVFVSCNFCGKSISYSCΞAVPHQGRGFSQYGVSG 300

AWRFWHKRAEFDIHRSKLDPSSKPLAQVFVSCNFCGKSISYSCSAVPHQGRGFSQYGVSG Sbjct: 241 AWRFWHKRAEFDIHRSKLDPSSKPLAQVFVSCNFCGKSISYSCSAVPHQGRGFSQYGVSG 300

Query: 301 SPTKSKVTSCPGCRKPLPRCALCLINMGTPVSSCPGGTKSDEKVDLSKDKKLAQFNNWFT 360

SPTKSKVTSCPGCRKPLPRCALCLINMGTPVSSCPGGTKSDEKVDLSKDKKLAQFNNWFT Sbjct: 301 SPTKSKVTSCPGCRKPLPRCALCLINMGTPVSSCPGGTKSDEKVDLSKDKKLAQFNNWFT 360

Query: 361 WCHNCRHGGHAGHMLSWFR 379

WCHNCRHGGHAGHMLSWFR Sbjct: 361 WCHNCRHGGHAGHMLSWFR 379

Pedant information for DKFZphtes3_7j8, frame 2

Report for DKFZphtes3_7j8.2

[LENGTH] 410

[MW] 45862.45

[pi] 6.51

[HOMOL] TREMBL:AC004982_1 gene: "WUGSC :H_DJ1159O04.1"; Homo sapiens PAC clone DJ1159O04 from 7p21-p22, complete sequence. 0.0

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YBL104c] 7e-48

[BLOCKS] BL00028 Zinc finger, C2H2 type, domain proteins

[BLOCKS] BL00534A Ferrochelatase proteins

[PIRKW] transmembrane protein 2e-46

[KW] All Alpha

SEQ MVESSRHNWSGLDKQSDIQNLNEERILALQLCGWIKKGTDVDVGPFLNSLVQEGEWERAA PRD cccccccccccccccccchhhhhhhhhhhhhhccccccccccccccccccccccchhhhh

SEQ AVALFNLDIRRAIQILNEGAΞSEKGDLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNN PRD hhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhccc

SEQ PYLCVMFAFLTSETGSYDGVLYENKVAVRDRVAFACKFLSDTQLNRYIEKLTNEMKEAGN

PRD ccccceeeccccccccccceeeccchhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhcc

SEQ LEGILLTGLTKDGVDLMESYVDRTGDVQTASYCMLQGSPLDVLKDERVQYWIENYRNLLD

PRD cceeeeeeccccchhhhhhhhcccccceeeeeccccccccccchhhhhhhhhhhhhhhhh

SEQ AWRFWHKRAEFDIHRSKLDPSSKPLAQVFVSCNFCGKSIΞYSCSAVPHQGRGFSQYGVSG

PRD hhhhhhhhhhhhhhcccccccccceeeeeeeccccccccccccccccccccccccccccc

SEQ SPTKSKVTSCPGCRKPLPRCALCLINMGTPVSSCPGGTKSDEKVDLSKDKKLAQFNNWFT

PRD ccccccccccccccccccceeeeecccccccccccccccccceeeehhhhhhhhhcceee

SEQ WCHNCRHGGHAGHMLSWFRDHAECPVSACTCKCMQLDTTGNLVPAETVQP

PRD eecccccccccchhhhhhhhhccccccccccccccccccccccccccccc

(No Prosite data available for DKFZphtes3_7j8.2) (No Pfam data available for DKFZphtes3_7j8.2) DKFZphtes3_7plO

group: Cell Cycle

DKFZphtes3_7plO.1 encodes a novel 422 ammo ac d putative protein, which is closely related to the Xenopus laevis XPMC2 protein.

In fission yeast the kinases Weel and Mikl control that initiation of mitosis starts after completion of DNA synthesis. Yeast in which both Weel and Mikl kinases are defective exhibit a mitotic catastrophe phenotype. XPMC2 of xenopus rescues several different yeast mitotic catastrophe mutants defective in Weel/Mikl kinase function. The XPMC2 protein is localised in the nucleus in Xenopus oocytes. The new protein is the human orthologue of this gene.

The new protein can find application in modulating/blocking the cell cycle. strong similarity to XPMC2 protein complete cDMA, complete eds, EST hits

Sequenced by BMFZ

Locus: /map="9q34"

Insert length: 2380 bp

Poly A stretch at pos. 2341, polyadenylation signal at pos. 2318

1 AGCGTGCGTG CTGAGGTATG CGCAACGCGT GCGGGGTCTC TTCCGGAGTC 51 TTTTCCTGGA CGGGGTCCCT GCGGTGGGTG TGTTTCGGCC TGGCCTGGGC

101 AGGCGCTTGT GCTGCCAGGG CGCCGGGCCC GGGGAGGCCG GGGTCTCGGG

151 TGGCCGCCGG CCCAGGCGCT GGACGGCAGC AGGATGGGGA AGGCGAAGGT

201 CCCCGCCTCC AAGCGCGCCC CGAGCAGCCC CGTGGCTAAG CCGGGTCCTG

251 TCAAGACGCT CACTCGGAAG AAAAACAAGA AGAAAAAAAG GTTTTGGAAA

301 AGCAAGGCGC GGGAAGTAAG CAAGAAGCCA GCAAGCGGCC CCGGTGCTGT

351 GGTGCGACCT CCAAAGGCAC CAGAAGACTT TTCTCAAAAC TGGAAGGCGC

401 TGCAAGAGTG GCTGCTGAAA CAAAAATCTC AGGCCCCAGA AAAGCCTCTT

451 GTCATCTCTC AGATGGGTTC CAAAAAGAAG CCCAAAATTA TCCAGCAAAA

501 CAAAAAAGAG ACCTCGCCTC AAGTGAAGGG AGAGGAGATG CCGGCAGGAA

551 AAGACCAGGA GGCCAGCAGG GGCTCTGTTC CTTCAGGTTC CAAGATGGAC

601 AGGAGGGCGC CAGTACCTCG CACCAAGGCC AGTGGAACAG AGCACAATAA

651 GAAAGGAACC AAGGAAAGGA CAAATGGTGA TATTGTTCCA GAACGAGGGG

701 ACATCGAGCA TAAGAAGCGG AAAGCTAAGG AGGCAGCCCC AGCCCCACCC

751 ACCGAGGAAG ACATCTGGTT TGACGACGTG GACCCAGCGG ATATCGAAGC

801 TGCCATAGGT CCAGAGGCGG CCAAGATAGC GAGGAAACAG TTGGGTCAGA

851 GCGAGGGCAG CGTCAGCCTC AGCCTCGTGA AAGAGCAGGC CTTCGGCGGC

901 CTGACAAGAG CCTTAGCCTT GGACTGTGAG ATGGTGGGCG TGGGCCCTAA

951 GGGGGAGGAG AGCATGGCCG CCCGTGTGTC CATCGTGAAC CAGTATGGGA 1001 AGTGCGTTTA TGACAAGTAC GTCAAACCAA CTGAGCCCGT GACGGACTAT 1051 AGGACAGCGG TCAGTGGGAT TCGGCCTGAG AACCTCAAGC AGGGAGAAGA 1101 GCTTGAAGTT GTTCAGAAGG AAGTGGCAGA GATGCTGAAG GGCAGAATTC 1151 TAGTGGGGCA CGCTCTGCAT AATGACCTAA AGGTACTATT TCTTGATCAT 1201 CCAAAAAAGA AGATTCGGGA CACACAGAAA TATAAACCTT TCAAGAGTCA 1251 AGTAAAGAGT GGAAGGCCGT CTCTGAGACT ACTTTCAGAG AAGATCCTTG 1301 GGCTCCAGGT CCAGCAGGCG GAGCACTGTT CAATTCAGGA TGCCCAGGCA 1351 GCAATGAGGC TGTACGTCAT GGTGAAGAAG GAGTGGGAGA GCATGGCCCG 1401 AGACAGGCGC CCCCTGCTGA CTGCTCCAGA CCACTGCAGT GACGACGCCT 1451 AGCAGTCCTG CCCTGCTGCT GCTGCCGCCC CGCTACAGAG GCAATGTGAC 1501 CAGTCACAGG GACAGATCAC ATCTCCCCAG AGTGGCAACT CTGGTGAAAC 1551 CTTTTCAGAA TCATGGCAGA GGGGCGTGGC GTGGTGCTAC TGAGAAGGTC 1601 CTCCTTCCTC TTGACTTTGT GGTCTGAAAC CTGGTCTTAC TGTCCATGTG 1651 TGTTTGGGCC CGGATGGTCA GGGTGGGGAG CAGGGACGGC CATGGGCACG 1701 CCTGGCCACG CTTTACCGAC TGCTGACCCC CTGGGCCAGG TGAGGTTGGG 1751 GCCTGTGGGC CGCCAGTCCA TACGGTGCTG TCACTGCCCA TCTTCGGTGA 1801 CACCCTGGGG TGAGGTGCTC AGCACCTTCC TCTCGAGGAG CCACATTTTC 1851 CTCCTTTGTG TTAGGGGACA TAACAAGCTC TGCTGGGCTT GAGGGACCCA 1901 GACCAGGTGT CTGCAGTCAG CTCCTGAGAC ACAGCTGGCC GGCACAACAG 1951 GTGTTACATC AGGGGTTTCC TGTGGCCGTT TGAACTTTGA GCATTTATCT 2001 AAATTAAATT GGCCCAGGGT TGGCTGGTGG GTCACCCAGC AGAGGCTTCT 2051 CCCCATAGCA CGAGGATGTG TTGCCTGGGC ACGGTGACTG CGGTTATTCC 2101 TGGAGGTCGG CAGACATGCC AACCTTGGGC TATTTGAGCT GGAGAAGCTA 2151 TGTGATGCTA GCCGGTGGCT TTCTGGGCTA GGCCCCAGTT TGAGGCTCCC 2201 CTGGGAACTA GAGCCAGGAA CAGCCAGTGG CACTGACAAG GGGACGGAGT 2251 CCAAGGCGTT ATTGGGCCAC CTGACAGCTG GACAGAAAAG GGGCAGACAC 2301 ACCGAGGATG CGATTTAAAA TAAATGCAGA TGTTTACTTG GAAAAAAAAA 2351 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

BLAST Results Entry HSAC2099 from database EMBL:

*** SEQUENCING IN PROGRESS *** Genomic sequence from Human 9q34; HTGS phase 1, 2 unordered pieces.

Score = 5055, P = O.Oe+00, identities = 1011/1011

8 exons Bp 104219-116190

Medline entries

95157530:

Cloning and expression of a Xenopus gene that prevents mitotic catastrophe in fission yeast.

Peptide information for frame 1

ORF from 184 bp to 1449 bp; peptide length: 422 Category: strong similarity to known protein

1 MGKAKVPASK RAPSSPVAKP GPVKTLTRKK NKKKKRFWKS KAREVSKKPA

51 SGPGAVVRPP KAPEDFSQNW KALQEWLLKQ KSQAPEKPLV ISQMGSKKKP

101 KIIQQNKKET SPQVKGEEMP AGKDQEASRG SVPSGSKMDR RAPVPRTKAS

151 GTEHNKKGTK ERTNGDIVPE RGDIEHKKRK AKEAAPAPPT EEDIWFDDVD

201 PADIEAAIGP EAAKIARKQL GQSEGSVSLS LVKEQAFGGL TRALALDCEM

251 VGVGPKGEES MAARVSIVNQ YGKCVYDKYV KPTEPVTDYR TAVSGIRPEN

301 LKQGEELEVV QKEVAEMLKG RILVGHALHN DLKVLFLDHP KKKIRDTQKY

351 KPFKSQVKSG RPSLRLLSEK ILGLQVQQAE HCSIQDAQAA MRLYVMVKKE

401 WESMARDRRP LLTAPDHCSD DA

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_7pl0, frame 1 No Alert BLASTP hits found

Pedant information for DKFZphtes3_7plO, frame 1

Report for DKFZphtes3_7plO .1

[LENGTH] 422

[MW] 46671.91

[pi] 9.79

[HOMOL] PIR:S53818 XPMC2 protein - African clawed frog 7e-96

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YOL080c] 2e-42

[FUNCAT] 01.03.16 polynucleotide degradation [S. cerevisiae, YGR276c] 2e-19

[FUNCAT] 05.04 translation (initiation, elongation and termination) [Ξ . cerevisiae,

YGL094C ] 7e-13

[FUNCAT] 04.05.05 mrna processing (5 '-end, 3 end processing and mrna degradation) [S. cerevisiae YGL094c] 7e-13

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YLR107w] 6e-10

[PROSITE] RGD 1

[PROSITE] MYRISTYL 4

[PROSITE] CAMP_PHOΞPHO_SITE 2

[PROSITE] CK2_PHOSPHO_SITE 6

[PROSITE] TYR_PHOSPHO_SITE 2

[PROSITE] GLYCOSAMINOGLYCAN 1

[PROSITE] PKC_PHOSPHO_SITE 8

[KW] All_Alpha

[KW] LOW COMPLEXITY 11.37 %

SEQ MGKAKVPASKRAPSSPVAKPGPVKTLTRKKNKKKKRFWKSKAREVSKKPASGPGAVVRPP

SEG xxxxxxxxxxxxxxxxxx

PRD cccccccccccccccccccccccccchhhhhhhhhhhhhhhhhccccccccccccccccc

SEQ KAPEDFSQNWKALQEWLLKQKSQAPEKPLVISQMGΞKKKPKIIQQNKKETSPQVKGEEMP

SEG xxxxxxxxxxxx

PRD cccccccchhhhhhhhhhhhhhhcccccccccccccccccceeeecccccccccccccee SEQ AGKDQEASRGSVPSGΞKMDRRAPVPRTKASGTEHNKKGTKERTNGDIVPERGDIEHKKRK SEG xxxxxx PRD ecccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhh

SEQ AKEAAPAPPTEEDIWFDDVDPADIEAAIGPEAAKIARKQLGQSEGSVSLSLVKEQAFGGL SEG xxxxxxxxxxxx PRD hhhhcccccccceeeecccccchhhhhhccchhhhhhhhhhcccccchhhhhhhhhhhhh

SEQ TRALALDCEMVGVGPKGEESMAARVSIVNQYGKCVYDKYVKPTEPVTDYRTAVSGIRPEN SEG PRD hhhcccccccccccccchhhhhhhhhccccccceeeeeeecccccccccccccccccccc

SEQ LKQGEELEVVQKEVAEMLKGRILVGHALHNDLKVLFLDHPKKKIRDTQKYKPFKSQVKSG SEG PRD ccccchhhhhhhhhhhhhhcceeeeccchhhhhhhhhcccccccccceeecccccccccc

SEQ RPSLRLLSEKILGLQVQQAEHCSIQDAQAAMRLYVMVKKEWESMARDRRPLLTAPDHCSD SEG PRD chhhhhhhhhhhhhhhccccccchhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccc

SEQ DA SEG PRD

Prosite for DKFZphtes3_7plO .1

PS00002 51->55 GLYCOSAMINOGLYCAN PDOC00002 PS00004 107->111 CAMP_PHOSPHO_SITE PDOC00004 PS00004 156->160 CAMP_PHOΞPHO_SITE PDOC00004 PS00005 9->12 PKC_PHOSPHO_SITE PDOC00005 PS00005 27->30 PKC_PHOSPHO_SITE PDOC00005 PS00005 46->49 PKC_PHOSPHO_SITE PDOC00005 PS00005 96->99 PKC_PHOSPHO_SITE PDOC00005 PS00005 347->350 PKC_PHOSPHO_SITE PDOC00005 PS00005 359->362 PKC_PHOSPHO_SITE PDOC00005 PS00005 363->366 PKC_PHOSPHO_SITE PDOC00005 PS00005 368->371 PKC_PHOSPHO_SITE PDOC00005 PS00006 136->140 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 150->154 CK2_PHOSPHO_SITE PDOC00006 PS00006 163->167 CK2_PHOSPHO_SITE PDOC00006 PS00006 190->194 CK2_PHOSPHO_SITE PDOC00006 PS00006 383->387 CK2_PHOSPHO_SITE PDOC00006 PS00006 413->417 CK2_PHOSPHO_SITE PDOC00006 PS00007 343->351 TYR_PHOSPHO_SITE PDOC00007 PS00007 342->351 TYR_PHOSPHO_SITE PDOC00007 PS00008 130->136 MYRISTYL PDOC00008 PS00008 151->157 MYRISTYL PDOC00008 PS00008 221->227 MYRISTYL PDOC00008 PS00008 239->245 MYRISTYL PDOC00008 PS00016 171->174 RGD PDOC00016

(No Pfam data available for DKFZphtes3_7plO .1)

DKFZphtes3_7p9

group: nucleic acid management

DKFZphtes3_7p9 encodes a novel 691 amino acid protein with similarity to human nuclear domain 10 protein NDP52.

The nuclear domain (ND)10 also described as POD or Kr bodies is involved in the development of acute promyelocytic leukemia and virus-host interactions. The NDP52 protein is part of this complex structure. In vivo, NDP52 is transcribed in all human tissues, but is redistributed upon viral infection and interferon treatment. ND10 plays an important role in the viral life cycle.

The novel protein is similar to NDP52. It contains three leucine zippers and a RGD cell attachment site. This protein seems to be a novel part of the ND819) complex.

The new protein can find application in modulation of viral infections and tumour events. similarity to nuclear domain 10 protein NDP52 complete cDNA, complete eds, EST hits

Sequenced by BMFZ

Locus: /map="329.1 cR from top of Chrl2 linkage group"

Insert length: 3003 bp

Poly A stretch at pos. 2957, no polyadenylation signal found

1 AAGGTGAGGG GAACAGCTGA TCCGTCTGTT GGGAGGACAG ATATCTCAAG

51 GCCAGGATGG AAGAATCACC ACTAAGCCGG GCACCATCCC GTGGTGGAGT

101 CAACTTTCTC AATGTAGCCC GGACCTACAT CCCCAACACC AAGGTGGAAT

151 GTCACTACAC CCTTCCCCCA GGCACCATGC CCAGTGCCAG TGACTGGATT

201 GGCATCTTCA AGGTGGAGGC TGCCTGTGTT CGGGATTACC ACACATTTGT

251 GTGGTCTTCC GTGCCTGAAA GTACAACTGA TGGTTCCCCC ATTCACACCA

301 GTGTCCAGTT CCAAGCCAGC TACCTGCCCA AACCAGGAGC TCAGCTCTAC

351 CAGTTCCGAT ATGTGAACCG CCAGGGCCAG GTGTGTGGGC AGAGCCCCCC

401 TTTCCAGTTC CGAGAGCCAA GGCCCATGGA TGAACTGGTG ACCCTGGAGG

451 AGGCTGATGG GGGCTCTGAC ATCCTGCTGG TTGTCCCCAA GGCAACTGTG

501 TTACAGAACC AGCTCGATGA GAGCCAGCAA GAACGGAATG ACCTGATGCA

551 GCTGAAGCTA CAGCTGGAGG GACAGGTGAC AGAGCTGAGG AGCCGAGTGC

601 AGGAGCTCGA GAGGGCTCTG GCAACTGCCA GGCAGGAGCA CACGGAGCTG

651 ATGGAACAGT ACAAGGGGAT TTCCCGGTCC CATGGGGAGA TCACAGAAGA

701 GAGGGACATC CTGAGCCGGC AACAGGGAGA CCATGTGGCA CGCATCCTGG

751 AGCTAGAGGA TGACATCCAG ACCATCAGTG AGAAAGTGCT GACGAAGGAA

801 GTGGAGCTGG ACAGGCTTAG AGACACAGTG AAGGCCCTGA CTCGGGAACA

851 AGAGAAGCTC CTTGGGCAAC TGAAAGAAGT ACAAGCAGAC AAGGAGCAAA

901 GTGAGGCTGA GCTCCAAGTG GCACAACAGG AGAACCATCA CTTAAATTTG

951 GACCTGAAGG AGGCGAAGAG CTGGCAAGAG GAGCAGAGTG CTCAGGCTCA

1001 GCGACTGAAA GACAAGGTGG CCCAGATGAA GGACACCCTA GGCCAGGCCC

1051 AGCAGCGGGT GGCCGAGCTG GAGCCCTTGA AGGAGCAGCT TCGAGGGGCC

1101 CAGGAGCTTG CAGCCTCAAG CCAGCAGAAA GCCACCCTTC TTGGGGAGGA

1151 GTTGGCCAGC GCAGCAGCAG CCAGGGACCG CACCATAGCC GAACTACACC

1201 GCAGCCGCCT GGAAGTGGCT GAAGTTAACG GCAGGCTGGC TGAGCTCGGT

1251 TTGCACTTGA AGGAAGAAAA ATGCCAATGG AGCAAGGAGC GGGCAGGGCT

1301 GCTGCAGAGT GTGGAGGCAG AGAAGGACAA GATCCTGAAG CTGAGTGCAG

1351 AGATACTTCG ATTGGAGAAG GCAGTTCAGG AGGAGAGGAC CCAAAACCAA

1401 GTGTTCAAGA CTGAGCTGGC CCGGGAGAAG GATTCTAGCC TGGTACAGTT

1451 GTCAGAAAGT AAGCGGGAGC TGACAGAGCT GCGGTCAGCC CTGCGTGTGC

1501 TCCAGAAGGA AAAGGAGCAG TTACAGGAGG AGAAACAGGA ATTGCTAGAG

1551 TACATGAGAA AGCTAGAGGC CCGCCTGGAG AAGGTGGCAG ATGAGAAGTG

1601 GAATGAGGAT GCCACCACAG AGGATGAGGA GGCCGCTGTG GGGCTGAGCT

1651 GCCCGGCAGC TCTGACAGAC TCAGAGGACG AGTCCCCAGA AGACATGAGG

1701 CTCCCACCCT ATGGCCTTTG TGAGCGTGGA GACCCAGGCT CCTCTCCTGC

1751 TGGGCCTCGA GAGGCTTCTC CCCTTGTTGT CATCAGCCAG CCGGCTCCCA

1801 TTTCTCCTCA CCTCTCTGGG CCAGCTGAGG ACAGTAGCTC TGACTCGGAG

1851 GCTGAAGATG AGAAGTCAGT CCTGATGGCA GCTGTGCAGA GTGGGGGTGA

1901 GGAGGCCAAC TTACTGCTTC CTGAACTGGG CAGTGCCTTC TATGACATGG

1951 CCAGTGGCTT TACAGTGGGT ACCCTGTCAG AAACCAGCAC TGGGGGCCCT

2001 GCCACCCCCA CATGGAAGGA GTGTCCTATC TGTAAGGAGC GCTTTCCTGC

2051 TGAGAGTGAC AAGGATGCCC TGGAGGACCA CATGGATGGA CACTTCTTTT

2101 TCAGCACCCA GGACCCCTTC ACCTTTGAGT GATCTTACTC CCTCGTACAT

2151 GCACAAATAC ACACTCATGC ACACACACAC TCACACACAT GCATACACTT

2201 AGGTTTCATG CCCATTTTCT ATCACACTGG GCTCCATGAT ATTCTGTTCC

2251 CTAAGAACTG CTTCTGTGTG CCCTGTTTTC ATCCCAAGAT TTCTCACTTC

2301 ATCCTCTCCT ACCTGGCTCT TTTGTCCCAG GGAGGGGTCC TGTTCGGAAG

2351 CAGTGGCTGA ATTTATCCCC TGAAAGTGGT TTTGGAGGAA CCGGGATGGA

2401 GGAGGCCTTC CCCTGTGGGA ATAGAATCGT CCACTCCTAG CCCTGGTTGC 2451 TTCTGATACA CAGCCACTGC ACACACACAC TCACACTCAC ACTCCCTTGT

2501 CTGATGCCCC AAAGCCAATT CCTGGGGCAC CCTACCCTCT CTTATTTGGA

2551 GTTTCCGTTG GTTTACCTGA GTTTTCTCTG GGGTCTGCAC AGAGGCAGCA

2601 GCATGGACAT CATGGCCTCT CAGGTCCCTT TTGGTTCTCA GTTTCATTGG

2651 TTCCTCTTTC TGTTCCCCCA TTGACTTCTG TGCCCCACCC TAGCCTTTTC

2701 CATAACCTTA GGTATTCAGT TTGGAGGGGT TTTTTGTATT TTTGAGGATT

2751 CCTGTATTCT GTATCCTCTC CTCGCATCTC CTCACATGGA AAGAAATAAT

2801 GTATTTGTGC CTTCTGTGAG GAATGGGGGG AACAAGTGGT CCCAGGTATC

2851 CCCATTTCCA AGGCCCCCCT CCCTCTCCAG GTCCCCCCAC AGCAATAAAA

2901 GCTTCCCCCT GATATCCATC CCTTTGTAGT TTGAACAAAT ATATTTATAT

2951 GATATGTAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA

3001 AAA

BLAST Results

Entry HS189353 from database EMBL: human STS WI-11261. Score = 2191, P = 1.4e-92, identities = 463/485

Medline entries

95310349:

Molecular characterization of NDP52, a novel protein of the nuclear domain 10, which is redistributed upon virus infection and interferon treatment.

97375672:

Cellular localization, expression, and structure of the nuclear dot protein 52.

Peptide information for frame 3

ORF from 57 bp to 2129 bp; peptide length: 691 Category: similarity to known protein Prosite motifs: RGD (557-560) LEUCINE_ZIPPER (163-185) LEUCINE_ZIPPER (475-497) LEUCINE ZIPPER (482-504)

1 MEESPLSRAP SRGGVNFLNV ARTYIPNTKV ECHYTLPPGT MPSASDWIGI

51 FKVEAACVRD YHTFVWSSVP ESTTDGSPIH TSVQFQASYL PKPGAQLYQF

101 RYVNRQGQVC GQSPPFQFRE PRPMDELVTL EEADGGSDIL LVVPKATVLQ

151 NQLDESQQER NDLMQLKLQL EGQVTELRSR VQELERALAT ARQEHTELME

201 QYKGISRSHG EITEERDILS RQQGDHVARI LELEDDIQTI SEKVLTKEVE

251 LDRLRDTVKA LTREQEKLLG QLKEVQADKE QSEAELQVAQ QENHHLNLDL

301 KEAKSWQEEQ SAQAQRLKDK VAQMKDTLGQ AQQRVAELEP LKEQLRGAQE

351 LAASSQQKAT LLGEELASAA AARDRTIAEL HRSRLEVAEV NGRLAELGLH

401 LKEEKCQWSK ERAGLLQSVE AEKDKILKLS AEILRLEKAV QEERTQNQVF

451 KTELAREKDS SLVQLSESKR ELTELRSALR VLQKEKEQLQ EEKQELLEYM

501 RKLEARLEKV ADEKWNEDAT TEDEEAAVGL SCPAALTDSE DESPEDMRLP

551 PYGLCERGDP GSSPAGPREA SPLVVISQPA PISPHLSGPA EDSSSDSEAE

601 DEKSVLMAAV QSGGEEANLL LPELGSAFYD MASGFTVGTL SETSTGGPAT

651 PTWKECPICK ERFPAESDKD ALEDHMDGHF FFSTQDPFTF E

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_7p9, frame 3

PIR:A56733 nuclear domain 10 protein NDP52 - human, N = 2, Score = 307, P = 7.7e-28

TREMBL :AB008852_1 gene: "NDP"; product: "NDP52"; Bos taurus mRNA for NDP52, complete eds., N = 2, Score = 302, P = 4e-27

TREMBL :AC004549_1 gene: "WUGSC :H_RG459N13.1"; product: "TXBP151"; Homo sapiens BAC clone RG459N13 from 7pl5, complete sequence., N = 2, Score = 275, P = 2.3e-25 PIR:G02043 TXBP151 - human, N = 2, Score = 270, P = 8.5e-25

TREMBL: DM35816_4 gene: "zip"; product: "nonmuscle myosin-II heavy chain"; Drosophila melanogaster nonmuscle myosin-II heavy chain (zip) gene, complete eds., N = 1, Score = 254, P = 1.4e-17

>PIR:A56733 nuclear domain 10 protein NDP52 - human Length = 446

HSPs:

Score = 307 (46.1 bits), Expect = 7.7e-28, Sum P(2) = 7.7e-28 Identities = 104/323 (32%), Positives = 158/323 (48%)

Query: 15 VNFLNVARTYIPNTKVECHYTLPPGTMPSASDWIGIFKVEAACVRDYHTFVWSSVPESTT 74

V F +V + YIP V CHYT +P DWIGIF+V R+Y+TF+W ++P Sbjct: 23 VIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYYTFMWVTLPIDLN 82

Query: 75 DGSPIHTSVQFQASYLPKPGAQLYQFRYVNRQGQVCGQSPPFQFREPRPMDELVTLEEAD 134

+ S VQF+A YLPK + YQF YV+ G V G S PFQFR D LV + Sbjct: 83 NKSAKQQEVQFKAYYLPKDD-EYYQFCYVDEDGVVRGASIPFQFRPENEEDILVVTTQ— 139

Query: 135 GGSDILLVVPKATVLQNQ-LDES QQERNDLMQLKLQLEGQVTE-LRSRVQELERALA 189

G + + K +NQ L +S Q++N MQ +LQ + + E L+S ++LE + Sbjct: 140 GEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETLQSINKKLELKVK 199

Query: 190 TARQE-HTELMEQYKGISRSHGEITEERDI-LSRQQGDHVARILELEDDIQTISEKVLTK 247

+ TEL+ Q K ++ E+ I + + Q + E+E +Q +K T+ Sbjct: 200 EQKDYWETELL-QLKEQNQKMSΞENEKMGIRVDQLQAQLSTQEKEMEKLVQGDQDK—TE 256

Query: 248 EVE-LDRLRDTVKALTREQEKLLGQLKEVQADKEQSEAELQVAQQENHHLNLDLKEAKSW 306

++E L + D + EQ K +L++ +Q+E QQE N DL + S Sbjct: 257 QLEQLKKENDHLFLSLTEQRKDQKKLEQTVEQMKQNETTAMKKQQELMDENFDLSKRLSE 316

Query: 307 QEEQSAQAQRLKDKVAQMKDTLGQAQQRV 335

E QR K+++ D L + R+ Sbjct: 317 NEIICNALQRQKERLEGENDLLKRENSRL 345

Score = 304 (45.6 bits), Expect = 2. le-27, Sum P(2) = 2. le-27 Identities = 98/337 (29%), Positives = 163/337 (48%)

Query: 15 VNFLNVARTYIPNTKVECHYTLPPGTMPSASDWIGIFKVEAACVRDYHTFVWSSVPESTT 74

V F +V + YIP V CHYT +P DWIGIF+V R+Y+TF+W ++P Sbjct: 23 VIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYYTFMWVTLPIDLN 82

Query: 75 DGSPIHTSVQFQASYLPKPGAQLYQFRYVNRQGQVCGQSPPFQFREPRPMDELVTLEEAD 134

+ S VQF+A YLPK + YQF YV+ G V G S PFQFR P +E Sbjct: 83 NKSAKQQEVQFKAYYLPKDD-EYYQFCYVDEDGVVRGASIPFQFR PENE 130

Query: 135 GGSDILLVVPKATVLQNQLDESQQERNDLMQLKLQLEGQVTELRSRVQELERALATARQE 194

DIL+V Q +++E +Q +L + +L+ L+ + +++ L +QE Sbjct: 131 --EDILVVTT QGEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQK-KQE 182

Query: 195 HTELMEQYKGISRSHGEITEERDILSRQQGDH-VARILELEDDIQTISEKVLTKEVELDR 253

E ++ I ++ ++ ++Q D+ +L+L++ Q +S + + +D+

Sbjct: 183 ELETLQS INKKLELKVKEQKDYWETELLQLKEQNQKMSSENEKMGIRVDQ 232

Query: 254 LRDTVKALTREQEKLL—GQLKEVQAD KEQSEAELQVAQQENHHLNLDLKEAKSWQE 308

L+ + +E EKL+ Q K Q + KE L + +Q L+ + Q Sbjct: 233 LQAQLSTQEKEMEKLVQGDQDKTEQLEQLKKENDHLFLSLTEQRKDQKKLEQTVEQMKQN 292

Query: 309 EQSA—QAQRLKDKVAQMKDTLGQAQQRVAELEPLKEQLRGAQEL 351

E +A + Q L D+ + L + + L+ KE+L G +L Sbjct: 293 ETTAMKKQQELMDENFDLSKRLSENEIICNALQRQKERLEGENDL 337

Score = 124 (18.6 bits), Expect = 2.3e-06, Sum P(2) = 2.3e-06 Identities = 53/227 (23%), Positives = 113/227 (49%)

Query: 138 DILLVVPKATVLQNQLDESQQERNDLMQLKLQLEGQVTELRSRVQELERALATARQEHTE 197

DIL+V Q +++E +Q +L + +L+ L+ + +++ L +QE E Sbjct: 132 DILVVTT QGEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQK-KQEELE 185

Query: 198 LMEQYKGISRSHGEITEERDILSRQQGDH-VARILELEDDIQTISEKVLTKEVELDRLRD 256

++ I ++ ++ ++Q D+ +L+L++ Q +S + + +D+L+

Sbjct: 186 TLQS INKKLELKVKEQKDYWETELLQLKEQNQKMSΞENEKMGIRVDQLQA 235

Query: 257 TVKALTREQEKLLGQLKEVQADKEQSEAELQVAQQENHHLNLDLKEAKSWQEEQSAQAQR 316

+ +E EKL VQ D++++E +L+ ++EN HL L L E + Q++ ++ Sbjct: 236 QLSTQEKEMEKL VQGDQDKTE-QLEQLKKENDHLFLSLTEQRKDQKKLEQTVEQ 288 Query: 317 LK-DKVAQMKDTLGQAQQRVAELEPLKEQLRGAQELA-ASSQQKATLLGE 364

+K ++ MK + Q+ + E L ++L + + A +QK L GE Sb_jct: 289 MKQNETTAMK KQQELMDENFDLSKRLSENEIICNALQRQKERLEGE 334

Score = 103 (15.5 bits), Expect = 4.4e-04, Sum P(2) = 4.4e-04 Identities = 63/278 (22%), Positives = 123/278 (44%)

Query: 299 DLKEAKSWQEEQSAQAQRLKDKVAQMK DTLGQAQQRVAELEPLKEQLRGAQELAAS 354

+++E + +E + Q LKD ++ D + Q++ ELE L + + EL Sbjct: 141 EVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETL-QSINKKLELKVK 199

Query: 355 SQQKATLLGEELASAAAARDRTIAELHRSRLEVAEVNGRLAELGLHLKEEKCQWSKERAG 414

Q+ EL + +E + + V ++ +L+ + E+ Q +++ Sbjct: 200 EQKD—YWETELLQLKEQNQKMΞSENEKMGIRVDQLQAQLSTQEKEM-EKLVQGDQDKTE 256

Query: 415 LLQSVEAEKDKI-LKLSAEIL RLEKAVQEERTQNQVFKTELAREKDSSLVQLSESKR 470

L+ ++ E D + L L+ + +LE+ V E+ QN+ T + ++++ SKR Sbjct: 257 QLEQLKKENDHLFLSLTEQRKDQKKLEQTV-EQMKQNET--TAMKKQQELMDENFDLSKR 313

Query: 471 ELTELRSALRVLQKEKEQLQEEKQELLEYMRKLEARLEKVADEKWNE DATTEDEEAA 527

L+E LQ++KE+L+ E +LL ++ +RL +N T DE A Sbjct: 314 -LSENEIICNALQRQKERLEGEN-DLL KRENSRLLSYMGLDFNSLPYQVPTSDEGGA 368

Query: 528 VGLSCPAALTD-SEDESPEDMRLPPYGLCERGDPGSSPAGPREASPL 573

GL+ + E SP + + +C+ D ++ PL Sbjct: 369 RQNPGLAYGNPYSGIQESSSPSPLSIKKCPICKADDICDHTLEQQQMQPL 418

Score = 64 (9.6 bits), Expect = 7.7e-28, Sum P(2) = 7.7e-28 Identities = 13/29 (44%), Positives = 17/29 (58%)

Query: 651 PTWKECPICKERFPAESDKDALEDHMDGH 679

P CPIC + FPA ++K EDH+ H Sbjct: 417 PLCFNCPICDKIFPA-TEKQIFEDHVFCH 444

Score = 64 (9.6 bits), Expect = 5.8e+00, Sum P(2) l.Oe+00 Identities = 26/90 (28%), Positives = 45/90 (50%)

Query: 470 RELTELRSALRVLQKEKEQLQEE KQELLEYMRKLEARLE-KVADEK—W 515

+E EL+ + LQK+ +Q E KQE LE ++ + +LE KV ++K W Sbjct: 154 KENQELKDSCISLQKQNSDMQAELQKKQEELETLQSINKKLELKVKEQKDYWETELLQLK 213 Query: 516 —NEDATTEDEEAAVGLS-CPAALTDSEDE 542 N+ ++E+E+ + + A L+ E E Sbjct: 214 EQNQKMSSENEKMGIRVDQLQAQLSTQEKE 243

Score = 47 (7.1 bits), Expect = 4.6e-26, Sum P(2) 4.6e-26 Identities = 11/30 (36%), Positives = 17/30 (56%)

Query: 631 MASGFTVGTLSETSTGGPATPTWKECPICK 660

+A G + E+S+ P + K+CPICK Sbjct: 374 LAYGNPYSGIQESSSPSPLSI—KKCPICK 401

Pedant information for DKFZphtes3_7p9, frame 3

Report for DKFZphtes3_7p9.3

[LENGTH] 691

[MW] 77336.52

[pi] 4.77

[HOMOL] PIR:A56733 nuclear domain 10 protein NDP52 - human 2e-29

[FUNCAT] 09.10 nuclear biogenesis [S. cerevisiae, YDR356w] 2e-ll

[FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YDR356w] 2e-ll

[FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL058w] 2e-ll

[FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YDR356w] 2e-ll

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDL058w] 2e-ll

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YLR309c] 2e-08

[FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YHR023w MYOl - myosm-1 isoform] 3e-07

[FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YHR023w MYOl - myosm-1 isoform] 3e-07

[FUNCAT] 03.25 cytokinesis [S. cerevisiae, YHR023w MYOl - myosιn-1 isoform] 3e-07

[FUNCAT] 09.13 biogenesis of chromosome structure [S. cerevisiae, YJL074c] 4e-07

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YNL250w] 4e-06

[FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [S. cerevisiae, YBR289w] 4e-06 [FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YBR289w]

4e-06

[FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YBR289w] 4e-06

[FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YNL250w] 4e-06

[FUNCAT] 03.13 meiosis [S. cerevisiae, YNL250w] 4e-06

[FUNCAT] 1 genome replication, transcription, recombination and repair [M. jannaschii, MJ1643] le-05

[FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YJR134c] 4e-05

[FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YKR095w] 4e-05

[ FUNCA ] 08.19 cellular import [S. cerevisiae, YNL243w] 7e-05

[ FUNCAT ] 01.03.16 polynucleotide degradation [S. cerevisiae, YNL243w] 7e-05

[FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YNL243w] 7e-05

[FUNCAT] 08.99 other intracellular-transport activities [Ξ. cerevisiae, YNL079c]

2e-04

[FUNCAT] 03.01 cell growth [S. cerevisiae, YNL079c] 2e-04

[BLOCKS] BL00682B ZP domain proteins

[EC] 3.6.1.32 Myosin ATPase le-13

[PIRKW] nucleus 6e-10

[PIRKW] phosphotransferase 2e-07

[PIRKW] duplication 9e-07

[PIRKW] citrulline le-09

[PIRKW] tandem repeat le-13

[PIRKW] heart 5e-ll

[PIRKW] endocytosis 5e-09

[PIRKW] polymorphism 3e-06

[PIRKW] cormfied cell envelope le-06

[PIRKW] transmembrane protein 6e-12

[PIRKW] serine/threomne-specific protein kinase 2e-07

[PIRKW] cell wall le-06

[PIRKW] zinc finger 5e-09

[PIRKW] metal binding 5e-09

[PIRKW] DNA binding 8e-08

[PIRKW] muscle contraction le-11

[PIRKW] IgG constant region-binding le-06

[PIRKW] acetylated amino end 4e-09

[PIRKW] actin binding le-13

[PIRKW] mitosis 9e-09

[PIRKW] microtubule binding 9e-09

[PIRKW] ATP le-13

[PIRKW] thick filament le-10

[PIRKW] phosphoprotem le-13

[PIRKW] epidermis le-06

[PIRKW] leucine zipper le-07

[PIRKW] glycoprotein 4e-07

[PIRKW] skeletal muscle 4e-10

[PIRKW] disulfide bond le-07

[PIRKW] calcium binding le-09

[PIRKW] alternative splicing le-10

[PIRKW] coiled coil le-13

[PIRKW] P-loop le-13

[PIRKW] heptad repeat 6e-10

[PIRKW] methylated amino acid le-13

[PIRKW] basement membrane 3e-06

[PIRKW] immunoglobulin receptor 2e-07

[PIRKW] peripheral membrane protein 5e-09

[PIRKW] dimer le-07

[PIRKW] cardiac muscle le-10

[PIRKW] extracellular matrix 3e-06

[PIRKW] hydrolase le-13

[PIRKW] microtubule 6e-10

[PIRKW] muscle 2e-09

[PIRKW] membrane protein 3e-06

[PIRKW] EF hand le-09

[PIRKW] cytoskeleton 6e-12

[PIRKW] hair le-09

[PIRKW] calmodulin binding 5e-09

[PIRKW] Golgi apparatus 3e-08

[SUPFAM] myosin heavy chain le-13

[SUPFAM] conserved hypothetical P115 protein le-08

[SUPFAM] hypothetical protein YJL074c 5e-07

[SUPFAM] centromere protein E 9e-09

[SUPFAM] unassigned Ser/Thr or Tyr-specific protein kinases 2e-07

[SUPFAM] calmodulin repeat homology le-09

[SUPFAM] myosin motor domain homology le-13

[SUPFAM] alpha-actinin actm-binding domain homology 3e-13

[SUPFAM] tropomyosin 3e-07

[SUPFAM] plectin 3e-13

[SUPFAM] trichohyalm le-09

[SUPFAM] pleckstrm repeat homology 4e-06

[SUPFAM] ribosomal protein S10 homology 3e-13 [SUPFAM] giantin 3e-08

[SUPFAM] protein kinase homology 2e-07

[SUPFAM] protein kinase C z c-binding repeat homology 4e-06

[SUPFAM] involucrin le-06

[SUPFAM] kinesin motor domain -homology 9e-09

[SUPFAM] human early endosome antigen 1 5e-09

[SUPFAM] unassigned kinesin-related proteins 8e-08

[SUPFAM] M5 protein 3e-08

[SUPFAM] cytoskeletal keratin 3e-08

[PROSITE] LEUCINE_ZIPPER 3

[PROSITE] RGD 1

[PROSITE] MYRISTYL 6

[PROSITE] CK2_PHOSPHO_SITE 25

[PROSITE] PKC_PHOSPHO_SITE 6

[KW] All_Alpha

[KW] LOW_COMPLEXITY 9.12 %

[KW] COILED COIL 39.36 %

SEQ MEESPLSRAPSRGGVNFLNVARTYIPNTKVECHYTLPPGTMPSASDWIGIFKVEAACVRD SEG PRD cccccccccccccceeeecceeeeeccccceeeeeccccccccccceeeeeeeeeecccc COILS

SEQ YHTFVWSSVPESTTDGSPIHTSVQFQASYLPKPGAQLYQFRYVNRQGQVCGQSPPFQFRE SEG PRD eeeeeeeecccccccccchhhhhhhhhhhhccccccceeeeecccccccccccccccccc COILS

SEQ PRPMDELVTLEEADGGSDILLVVPKATVLQNQLDESQQERNDLMQLKLQLEGQVTELRSR SEG PRD cccccceeehhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ VQELERALATARQEHTELMEQYKGISRSHGEITEERDILSRQQGDHVARILELEDDIQTI SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ ΞEKVLTKEVELDRLRDTVKALTREQEKLLGQLKEVQADKEQSEAELQVAQQENHHLNLDL SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ KEAKSWQEEQSAQAQRLKDKVAQMKDTLGQAQQRVAELEPLKEQLRGAQELAASSQQKAT

SEG XX

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCC .. CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC . CCCCCCCCCCCCCCCCCCCC

SEQ LLGEELASAAAARDRTIAELHRSRLEVAEVNGRLAELGLHLKEEKCQWSKERAGLLQSVE

SEG xxxxxxxxxxxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCC CCCCCCCCCCC

SEQ AEKDKILKLΞAEILRLEKAVQEERTQNQVFKTELAREKDSSLVQLSESKRELTELRSALR SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCC CCCCCCCCCCCCCCCCCCC

SEQ VLQKEKEQLQEEKQELLEYMRKLEARLEKVADEKWNEDATTEDEEAAVGLSCPAALTDSE

SEG . xxxxxxxxxxxxxxxxx xxxxxxxxxxx

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ DESPEDMRLPPYGLCERGDPGSSPAGPREASPLVVISQPAPISPHLSGPAEDSSSDSEAE SEG xxxxxxxxxxx PRD hhhhccccccccccccccccccccccccccceeeeeeccccccccccccccccccccchh COILS

SEQ DEKSVLMAAVQSGGEEANLLLPELGSAFYDMASGFTVGTLSETSTGGPATPTWKECPICK SEG xx PRD hhhhhhhhhhhhcccccccccccccccccccccccccccccccccccccccccccccccc COILS

SEQ ERFPAESDKDALEDHMDGHFFFSTQDPFTFE SEG PRD cccccccchhhhhhhccccceeecccccccc COILS

DKFZphtes3_8e24

group: signal transduction

DKFZphtes3_8e24.3 encodes a novel 658 amino acid putative GTP-binding protein, related to yeast YGL099w and mouse MMR1 putative GTP-binding proteins.

GTP-bmding proteins are involved m various signal transduction pathways, transferring the signal of a cellular receptor to an intracellular signal cascade.

The new protein can find clinical application in modulating/blocking the response to a cellular receptor. strong similarity to guanine nucleotide binding proteins complete cDNA, complete eds, potential start at Bp 31, EST hits

Sequenced by MediGenomix

Locus: unknown

Insert length: 3290 bp

Poly A stretch at pos. 3269, polyadenylation signal at pos. 3251

1 CGTCCAGCGG TCGTGTTGCC ATGGGCCGGA GGAGAGCCCC GGCCGGTGGG 51 TCGCTGGGAC GGGCCCTTAT GCGCCATCAG ACTCAGCGGA GCCGAAGCCA

101 TCGTCACACT GACTCCTGGT TGCACACAAG TGAACTCAAT GATGGCTATG

151 ATTGGGGTCG TCTTAATCTT CAGTCAGTGA CTGAACAGAG CTCCCTTGAT

201 GACTTCCTTG CTACTGCAGA ACTTGCAGGA ACAGAGTTTG TAGCTGAAAA

251 ACTTAATATT AAGTTTGTGC CTGCTGAGGC TAGAACTGGA CTACTGTCTT

301 TCGAGGAGAG CCAGAGAATT AAGAAGCTCC ATGAAGAAAA CAAACAGTTC

351 TTGTGTATAC CGAGGAGACC AAACTGGAAC CAAAATACTA CCCCAGAAGA

401 ACTCAAACAA GCAGAGAAAG ATAACTTTCT AGAATGGAGA CGTCAGCTTG

451 TCCGGCTAGA AGAGGAACAG AAGCTGATAT TGACTCCATT TGAACGAAAT

501 TTGGACTTTT GGCGCCAGCT CTGGAGAGTC ATTGAGAGAA GTGATATTGT

551 GGTCCAGATA GTAGATGCTC GAAACCCACT CCTGTTTAGA TGTGAGGATT

601 TGGAATGTTA TGTGAAAGAA ATGGATGCCA ATAAGGAGAA CGTCATTCTG

651 ATCAACAAGG CAGACTTGCT GACTGCTGAG CAGCGGAGTG CCTGGGCCAT

701 GTACTTCGAA AAAGAAGATG TGAAGGTTAT TTTCTGGTCA GCTTTGGCCG

751 GAGCCATTCC CCTGAATGGT GACTCTGAGG AAGAGGCAAA CAGAGATGAT

801 AGACAAAGCA ACACAACTGA GTTTGGACAT TCCAGTTTCG ACCAGGCTGA

851 AATTTCCCAC AGTGAATCCG AACATCTCCC AGCTAGGGAT TCTCCTTCAC

901 TTAGTGAAAA TCCCACAACG GATGAAGATG ACAGTGAGTA TGAGGACTGT

951 CCAGAGGAGG AGGAAGACGA CTGGCAGACG TGCTCAGAAG AAGACGGTCC 1001 CAAGGAAGAG GACTGCAGCC AGGACTGGAA GGAAAGCTCT ACTGCAGATT 1051 CTGAGGCTCG GAGCAGGAAA ACCCCACAGA AGAGGCAGAT ACACAATTTT 1101 AGCCATCTGG TATCCAAGCA GGAGTTACTG GAGCTCTTTA AGGAGCTACA 1151 CACTGGGAGA AAGGTGAAAG ATGGGCAACT TACGGTCGGA CTGGTGGGCT 1201 ACCCTAATGT TGGTAAGAGT TCAACAATCA ACACCATCAT GGGCAACAAG 1251 AAAGTATCTG TGTCTGCCAC ACCTGGTCAC ACAAAGCACT TTCAGACTCT 1301 CTATGTGGAG CCTGGCCTCT GCCTGTGTGA CTGTCCTGGC TTGGTGATGC 1351 CATCTTTTGT GTCTACCAAG GCAGAAATGA CTTGCAGCGG AATCCTCCCA 1401 ATTGATCAGA TGAGAGATCA TGTTCCTCCT GTATCACTAG TTTGCCAGAA 1451 TATTCCAAGA CATGTTTTAG AAGCTACCTA TGGCATTAAC ATCATAACGC 1501 CTAGAGAGGA TGAAGATCCC CACCGACCTC CAACATCGGA AGAACTGTTG 1551 ACAGCTTATG GATACATGCG AGGATTCATG ACAGCGCATG GACAGCCAGA 1601 CCAGCCTCGA TCTGCGCGCT ACATCCTGAA GGACTATGTC AGTGGTAAGC 1651 TGCTGTACTG CCATCCTCCT CCTGGAAGAG ATCCTGTAAC TTTTCAGCAT 1701 CAACACCAGC GACTCCTAGA GAACAAAATG AACAGTGATG AAATAAAAAT 1751 GCAGCTAGGC AGAAATAAAA AAGCAAAGCA GATTGAAAAT ATCGTTGACA 1801 AAACTTTTTT CCATCAAGAG AATGTGAGGG CTTTGACCAA AGGAGTCCAG 1851 GCTGTGATGG GTTACAAGCC CGGGAGTGGT GTAGTGACTG CATCCACTGC 1901 GAGCTCTGAG AACGGGGCGG GGAAGCCCTG GAAAAAACAT GGCAACAGAA 1951 ATAAAAAAGA AAAAAGTCGT AGACTCTACA AGCACCTGGA TATGTGAGGT 2001 TGGGCTGCAA CAGAAATGTC ATCTGCATTG TGCAGATGGA AAAGAGCAGA 2051 AGCTGCCTGT TGCCTGTGGA ACTGTCCCAA GACACTAGCA CTGTAGAACG 2101 GGCCCTGCTC TTGCAGAGCA CGGCTGCACC CAACAGTCTC CATGTCAAGA 2151 CCAAGGGCCT CCTGGAAACA CCAGCTCTGA CAAAAAGGAG TCATCTGGGA 2201 GCCCGAGAAT CCTACTCCTG GCCGGGCACA GTGGCTCACG CACCAACATG 2251 GAGAAACCCC GTCTCTACTA AAAATACAAA AAAATTAGCC AGGCGTGGTG 2301 GCGCGCACCT GTAATCCCAG CTACTCGGGA GGCTGAGGCA GGAGAATCAC 2351 TTGAACCAGG GAGGCAGAGT TTGCAGTGAA TGGAGATTGC GCCGCTGCAC 2401 TCCAGCCTGG GCGACAGAGT GAGACTGCAT CACAAGAAAA AAAATTTGCA 2451 AGGGATGGTT CACGAGACAC ATTTGGGACG AAGGTGAAAG AGAAATTCCC 2501 CATTCTGAGT GTCCTAGTTG GGTTCCTCCG ACTCTAAACA AGGGACTTGG 2551 GTTCAGTTAG TGTACAGCGG GGGCTCACGT CCACTAAGGA ACATGTAGAA 2601 TGTAACCACC GGGTGACAGG GAAGCTGCGG TATTTACTAC CTAGCCCCCA 2651 TCTTCACTGG TTATTCCACT TATTTAAAAT GTCCAGAATA AGCAAATCTC

2701 CATATAGAGG AAGTAGATTA GTGGTTGCTT CGGGATGGGA GGAATGGGAA

2751 GATTGAGGTC TTTCTTTTGC AGTGATAAAA ATGTCCTAAA ATTGACTGTA

2801 GCGATGGTCA CACAACTCTG AATATGCTTA AGACCATTGA ATTACACACT

2851 TTACGTTGGT GAATTGTATG GTATGTAAAT TATAGTTCAA TAACATAGTT

2901 ACAAAAGATA ATCAAAAGCA TGAAAGCACT ATTGATGTGG TTTGGATCTG

2951 TGTCCTCACC GAGTCTCATG TTGAAATGTA AGCCCCCTGG TGGGAGGCGA

3001 TGGGATTATG GGGCAGAGTC CTCACAAACG GTTTAGCACC ACCCGCTCAG

3051 TGCTGTTCTC CTGATATTGA GTCCTCATCA CATCTGGTTG CTTCAAAGTG

3101 TGTGGTGCCT CCCCTCTGTC TCCCTCCTGC TCTGGCCATA TAAGATGTGC

3151 CTGCTTCTCC TTCGCCTTCT AACATGATTG TAAGTTTCCT GAGGCCTCCC

3201 TAGAAGCAAA AGCTGCTGTG CTTCCTGTAC CATCTACTGG ACCGTGAGCC

3251 AATTAAACCT CTTTTCTTTA TAAAAAAAAA AAAAAAAAGG

BLAST Results

No BLAST result

Medline entries

No Medlme entry

Peptide information for frame 3

ORF from 21 bp to 1994 bp; peptide length: 658 Category: strong similarity to known protein

1 MGRRRAPAGG SLGRALMRHQ TQRSRSHRHT DΞWLHTSELN DGYDWGRLNL

51 QSVTEQSSLD DFLATAELAG TEFVAEKLNI KFVPAEARTG LLSFEEΞQRI

101 KKLHEENKQF LCIPRRPNWN QNTTPEELKQ AEKDNFLEWR RQLVRLEEEQ

151 KLILTPFERN LDFWRQLWRV IERSDIVVQI VDARNPLLFR CEDLECYVKE

201 MDANKENVIL INKADLLTAE QRSAWAMYFE KEDVKVIFWS ALAGAIPLNG

251 DSEEEANRDD RQSNTTEFGH SSFDQAEISH SESEHLPARD SPSLSENPTT

301 DEDDSEYEDC PEEEEDDWQT CSEEDGPKEE DCSQDWKESS TADSEARSRK

351 TPQKRQIHNF SHLVSKQELL ELFKELHTGR KVKDGQLTVG LVGYPNVGKS

401 STINTIMGNK KVSVSATPGH TKHFQTLYVE PGLCLCDCPG LVMPSFVSTK

451 AEMTCSGILP IDQMRDHVPP VSLVCQNIPR HVLEATYGIN IITPREDEDP

501 HRPPTSEELL TAYGYMRGFM TAHGQPDQPR SARYILKDYV SGKLLYCHPP

551 PGRDPVTFQH QHQRLLENKM NSDEIKMQLG RNKKAKQIEN IVDKTFFHQE

601 NVRALTKGVQ AVMGYKPGSG VVTASTASSE NGAGKPWKKH GNRNKKEKSR 651 RLYKHLDM

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_8e24, frame 3

SWISSPROT :YAWG_SCHPO HYPOTHETICAL GTP-BINDING PROTEIN C3F10.16C IN CHROMOSOME I., N = 3, Score = 560, P = 1.6e-lll

PIR:S64106 hypothetical protein YGL099w - yeast (Saccharomyces cerevisiae), N = 2, Score = 544, P = 2.6e-105

TREMBL :CEAF3143_1 gene: "C53H9.2"; Caenorhabditis elegans cosmid C53H9., N = 1, Score = 551, P = 2.9e-53

SWISSPROT :MMRl_MOUSE POSSIBLE GTP-BINDING PROTEIN MMR1. , N = 2, Score = 311, P = 7.5e-31

>SWISSPROT:YAWG_SCHPO HYPOTHETICAL GTP-BINDING PROTEIN C3F10.16C IN CHROMOSOME I.

Length = 616

HSPs:

Score = 560 (84.0 bits), Expect = 1.6e-lll, Sum P(3) = 1.6e-lll Identities = 119/253 (47%), Positives = 163/253 (64%)

Query: 12 LGRALMRHQTQRSRSHRHTDSWLHTSELNDGYDWGRLNLQSVTEQSSLDDFLATAELAGT 71 LGRA+ T+ R+ + H + + R L+SVT ++ LD+FL TAEL Sbjct: 12 LGRAIQSDFTKNRRNRK—GGLKHIVDSDPKAH—RAALRSVTHETDLDEFLNTAELGEV 67

Query: 72 EFVAEKLNIKFVP-AEARTGLLSFEESQRIKKLHEENKQFLCIPRRPNWNQNTTPEELKQ 130

EF+AEK N+ + E LLS EE+ R K+ E+NK L IPRRP+W+Q TT EL + Sb_jct: 68 EFIAEKQNVTVIQNPEQNPFLLSKEEAARSKQKQEKNKDRLTIPRRPHWDQTTTAVELDR 127

Query: 131 AEKDNFLEWRRQLVRLEEEQKLILTPFERNLDFWRQLWRVIERSDIVVQIVDARNPLLFR 190

E+++FL WRR L +L++ + I+TPFERNL+ WRQLWRVIERSD+VVQIVDARNPL FR Sb_jct: 128 MEREΞFLNWRRNLAQLQDVEGFIVTPFERNLEIWRQLWRVIERSDVVVQIVDARNPLFFR 187

Query: 191 CEDLECYVKEMDANKENVILINKADLLTAEQRSAWAMYFEKEDVKVIFWSALAGAIPLNG 250

LE YVKE+ +K+N +L+NKAD+LT EQR+ W+ YF + ++ +F+SA A N Sbjct: 188 SAHLEQYVKEVGPSKKNFLLVNKADMLTEEQRNYWSSYFNENNIPFLFFSARMAA-EANE 246

Query: 251 DSEEEANRDDRQSN 264

E+ + SN Sbjct: 247 RGEDLETYESTSSN 260

Score = 532 (79.8 bits), Expect = 1.6e-lll, Sum P(3) = 1.6e-lll Identities = 131/323 (40%), Positives = 192/323 (59%)

Query: 340 STADSEARSRKTPQKRQIHNFSHLVΞKQELLELFKELHTGRKVKDGQ—LTVGLVGYPNV 397

ST+ +E + +H+ S + + + L +F++ + + DG+ +T GLVGYPNV Sbjct: 256 STSSNEIPESLQADENDVHS-SRIATLKVLEGIFEKFAS—TLPDGKTKMTFGLVGYPNV 312

Query: 398 GKSSTINTIMGNKKVSVSATPGHTKHFQTLYVEPGLCLCDCPGLVMPSFVΞTKAEMTCSG 457

GKSSTIN ++G+KKVSVS+TPG TKHFQT+ + + L DCPGLV PSF +T+A++ G Sbjct: 313 GKSSTINALVGSKKVSVSΞTPGKTKHFQTINLSEKVSLLDCPGLVFPSFATTQADLVLDG 372

Query: 458 ILPIDQMRDHVPPVSLVCQNIPRHVLEATYGINI-ITPREDEDPHRPPTSEELLTAYGYM 516

+LPIDQ+R++ P +L+ + IP+ VLE Y I I I P E E P+++E+L + Sbjct: 373 VLPIDQLREYTGPSALMAERIPKEVLETLYTIRIRIKPIE-EGGTGVPSAQEVLFPFARS 431

Query: 517 RGFMTAH-GQPDQPRSARYILKDYVSGKLLYCHPPPG—RDPVTFQHQHQRLLENKMNSD 573

RGFM AH G PD R+AR +LKDYV+GKLLY HPPP F +H + + + SD Sbjct: 432 RGFMRAHHGTPDDSRAARILLKDYVNGKLLYVHPPPNYPNSGSEFNKEHHQKIVSA-TSD 490

Query: 574 EIKMQLGR NKKAKQIEN-IVDKTFFHQEN—VRALTKGVQAVM-G—YKPGSGVVTA 624

I +L R + E+ +VD +F QEN VR + KG M G YK + + Sbjct: 491 SITEKLQRTAISDNTLSAESQLVDDEYF-QENPHVRPMVKGTAVAMQGPVYKGRNTMQPF 549

Query: 625 STASSENGAGK-PWKKHGNRNKKEKSRRL 652

+++ + K P G + K+R+L Sbjct: 550 QRRLNDDASPKYPMNAQGKPLSRRKARQL 578

Score = 47 (7.1 bits), Expect = 1.3e-60, Sum P(3) = 1.3e-60 Identities = 21/84 (25%), Positives = 35/84 (41%)

Query: 552 GRDPVTFQHQHQRLLENKMNSDEIKMQLGRNKKAKQIENIVDKTFFHQENVRALTKGVQA 611

G D T++ + + +DE + R K +E I +K F TK Sbjct: 248 GEDLETYESTSSNEIPESLQADENDVHSSRIATLKVLEGIFEK—FASTLPDGKTKMTFG 305

Query: 612 VMGYKPGSGVVTASTAΞSENGAGK 635

++GY P G +ST ++ G+ K Sbjct: 306 LVGY-PNVG—KSSTINALVGSKK 326

Score = 43 (6.5 bits). Expect = 1.6e-lll, Sum P(3) = 1.6e-lll Identities = 7/13 (53%), Positives = 9/13 (69%)

Query: 638 KKHGNRNKKEKSR 650

KKH +NK+ K R Sbjct: 596 KKHNKKNKRSKQR 608

Pedant information for DKFZphtes3_8e24, frame 3

Report for DKFZphtes3_8e24.3

[LENGTH] 658

[MW] 75226.58

[pi] 5.86

[HOMOL] SWISSPROT :YAWG_SCHPO HYPOTHETICAL GTP-BINDING PROTEIN C3F10.16C IN CHROMOSOME

I. 5e-56

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YGL099w] 3e-55

[FUNCAT] r general function prediction [M. jannaschii, MJ1464] le-16

[FUNCAT] 08.16 extracellular transport [S. cerevisiae, YER006w] 3e-09

[PIRKW] P-loop le-27

[PIRKW] GTP binding le-27

[SUPFAM] conserved hypothetical protein MG442 7e-08 [PROSITE] ATP_GTP_A 1

[PROSITE] MYRISTYL 3

[PROSITE] AMIDATION 2

[PROSITE] CAMP_PHOSPHO_SITE 1

[PROSITE] CK2_PHOSPHO_SITE 19

[PROSITE] TYR_PHOSPHO_SITE 2

[PROSITE] PKC_PHOSPHO_SITE 10

[PROSITE] ASN_GLYCOSYLATION 2

[KW] Alpha_Beta

[KW] LOW COMPLEXITY 4.56 %

SEQ MGRRRAPAGGSLGRALMRHQTQRSRSHRHTDSWLHTSELNDGYDWGRLNLQSVTEQSSLD SEG xxxxxxxxxxxxx PRD cccccccccccchhhhhhhhhhhccccccccccccccccccccccchhhhhhhhccccch

SEQ DFLATAELAGTEFVAEKLNIKFVPAEARTGLLSFEESQRIKKLHEENKQFLCIPRRPNWN SEG PRD hhhhhhhhhhheeeecccceeeeeeccccccchhhhhhhhhhhhhhhhhhhccccccccc

SEQ QNTTPEELKQAEKDNFLEWRRQLVRLEEEQKLILTPFERNLDFWRQLWRVIERSDIVVQI SEG PRD cccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhhcceeeee

SEQ VDARNPLLFRCEDLECYVKEMDANKENVILINKADLLTAEQRSAWAMYFEKEDVKVIFWS SEG PRD eccccccccchhhhhhhhhhhccccceeeeecccchhhhhhhhhhhhhhhhccceeeeec

SEQ ALAGAIPLNGDSEEEANRDDRQSNTTEFGHSSFDQAEISHSESEHLPARDSPSLSENPTT SEG PRD cccccccccccchhhhhhhhhhcccccccccccccccccccccccccccccccccccccc

SEQ DEDDSEYEDCPEEEEDDWQTCSEEDGPKEEDCSQDWKESSTADSEARΞRKTPQKRQIHNF SEG xxxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccchhhhhhhhhccccccccccc

SEQ SHLVSKQELLELFKELHTGRKVKDGQLTVGLVGYPNVGKSSTINTIMGNKKVSVSATPGH SEG PRD ccccchhhhhhhhhhhhhhhccccceeeeeecccccccccceeeeccccceeeeeccccc

SEQ TKHFQTLYVEPGLCLCDCPGLVMPSFVSTKAEMTCSGILPIDQMRDHVPPVSLVCQNIPR SEG PRD cceeeeeeeccceeecccccccccccchhhhhhhhccccccccccccccceeeeecccch

SEQ HVLEATYGINIITPREDEDPHRPPTSEELLTAYGYMRGFMTAHGQPDQPRSARYILKDYV SEG PRD hhhhhhhhccccccccccccccccchhhhhhhhhhhhhhcccccccccchhhhhhhhhcc

SEQ SGKLLYCHPPPGRDPVTFQHQHQRLLENKMNSDEIKMQLGRNKKAKQIENIVDKTFFHQE SEG PRD ccceeeeccccccccccchhhhhhhhhhcccchhhhhhhhcchhhhhhhhhhhhccccch

SEQ NVRALTKGVQAVMGYKPGSGVVTASTASSENGAGKPWKKHGNRNKKEKSRRLYKHLDM SEG PRD hhhhhhhceeeeeecccccceeecccccccccccccccccccccchhhhhhhhhhccc

Prosite for DKFZphtes3_8e24.3

PS00001 264->268 ASN_GLYCOSYLATION PDOC00001 PS00001 359->363 ASN_GLYCOSYLATION PDOC00001 PS00004 410->414 CAMP_PHOSPHO_SITE PDOC00004 PS00005 21->24 PKC_PHOSPHO_SITE PDOC00005 PS00005 26->29 PKC_PHOSPHO_SITE PDOC00005 PS00005 97->100 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 348->351 PKC_PHOSPHO_SITE PDOC00005 PS00005 378->381 PKC_PHOΞPHO_SITE PDOC00005 PS00005 448->451 PKC_PHOSPHO_SITE PDOC00005 PS00005 493->496 PKC_PHOSPHO_SITE PDOC00005 PS00005 531->534 PKC_PHOSPHO_SITE PDOC00005 PS00005 541->544 PKC_PHOSPHO_SITE PDOC00005 PS00005 649->652 PKC_PHOSPHO_SITE PDOC00005 PS00006 52->56 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 57->61 CK2_PHOSPHO_SITE PDOC00006 PS00006 93->97 CK2_PHOSPHO_SITE PDOC00006 PS00006 123->127 CK2_PHOSPHO_SITE PDOC00006 PS00006 155->159 CK2_PHOSPHO_SITE PDOC00006 PS00006 252->256 CK2_PHOSPHO_SITE PDOC00006 PS00006 271->275 CK2_PHOSPHO_SITE PDOC00006 PS00006 279->283 CK2 PHOSPHO SITE PDOC00006

so Os 0 0 σ α σ o σ σ σ σ σ o σ σ o σ o o

0 O O O O 0000000000000

0 0000000000000

DKFZphtes3_8gll

group: testes derived

DKFZphtes3_8gll encodes a novel proline-rich 939 amino acid protein without similarity to known proteins.

The novel protein contains an ATP/GTP-bindmg site motif A (P-loop) .

No informative BLAST results; No predictive prosite, pfam or - SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . unknown, prolin ritch protein

1 EST hit (from testis library)

Sequenced by MediGenomix

Locus : unknown

Insert length: 3100 bp

Poly A stretch at pos. 3056, polyadenylation signal at pos. 3041

1 AGAGTCTTCC CTCAGCATAT TTTACGATAG AGAAGATCTT GTTCCAATGG

51 AAGAAAGTGA GGACTCACAG AGTGATTCCC AGACAAGGAT TTCTGAGTCC

101 CAACACTCCC TCAAGCCAAA TTATCTTTCC CAGGCCAAGA CTGACTTCTC

151 AGAACAGTTC CAGTTGCTAG AAGATCTGCA GCTAAAAATA GCAGCAAAAC

201 TCTTAAGGAG TCAAATACCC CCCGATGTGC CTCCACCTCT AGCTTCAGGT

251 CTAGTCCTAA AATACCCTAT CTGCCTACAG TGTGGCCGAT GTTCAGGACT

301 TAATTGCCAT CATAAATTAC AGACCACTTC GGGGCCTTAT CTTCTTATCT

351 ATCCACAGCT CCACCTTGTA CGCACTCCTG AAGGCCATGG TGAGGTTCGG

401 TTGCATCTTG GCTTTAGGCT GAGAATTGGG AAAAGATCCC AAATCTCAAA

451 GTATCGTGAA AGAGATAGAC CCGTCATACG GAGAAGCCCT ATATCACCAT

501 CACAAAGGAA AGCTAAAATC TATACTCAAG CTTCCAAGAG TCCTACTTCC

551 ACAATAGATT TGCAGTCTGG GCCTTCCCAG TCCCCTGCTC CTGTACAAGT

601 CTACATCAGG CGAGGACAAC GCAGCAGGCC TGACTTAGTA GAAAAGACAA

651 AAACTAGAGC ACCTGGGCAC TATGAATTCA CTCAAGTTCA CAACCTACCA

701 GAGAGTGACT CTGAAAGCAC TCAGAATGAA AAACGGGCTA AAGTGAGAAC

751 CAAAAAGACC TCTGATTCAA AATATCCAAT GAAGAGAATC ACCAAGCGAC

801 TTAGAAAACA CAGAAAGTTC TACACAAACA GTAGAACCAC AATAGAGAGT

851 CCTTCTAGGG AATTAGCAGC CCATTTAAGA AGGAAGAGGA TTGGAGCAAC

901 TCAGACAAGT ACTGCCTCTT TAAAAAGACA ACCTAAGAAA CCTTCCCAAC

951 CCAAGTTCAT GCAACTGCTT TTTCAGAGCC TAAAGCGGGC ATTCCAAACA

1001 GCACACAGAG TTATAGCTTC TGTTGGGCGG AAGCCTGTGG ACGGGACAAG

1051 GCCAGACAAT TTGTGGGCAA GCAAAAACTA TTATCCAAAA CAAAATGCCA

1101 GGGACTATTG CTTACCAAGC AGTATCAAAA GAGACAAGAG GTCAGCTGAC

1151 AAGCTAACGC CAGCAGGCTC AACCATTAAG CAGGAGGACA TATTGTGGGG

1201 AGGAACGGTC CAGTGCAGAT CAGCTCAACA GCCAAGAAGA GCTTACTCTT

1251 TCCAACCCAG ACCTCTTCGA CTGCCCAAGC CCACAGATTC CCAAAGTGGT

1301 ATTGCTTTCC AAACTGCCTC AGTGGGGCAG CCTCTGAGAA CTGTTCAAAA

1351 GGACAGTAGT AGCAGATCAA AGAAAAACTT CTATAGAAAT GAAACCTCCA

1401 GCCAGGAGTC TAAGAACTTG TCCACACCAG GAACCAGAGT TCAGGCCCGA

1451 GGAAGAATCC TACCTGGTTC CCCTGTGAAG AGAACCTGGC ACCGACATCT

1501 TAAAGACAAA CTCACACACA AGGAGCATAA CCACCCCAGC TTCTATAGGG

1551 AGAGAACCCC ACGCGGTCCT TCTGAGAGAA CCCGTCATAA CCCCTCTTGG

1601 AGAAACCATC GCAGTCCCTC TGAGAGAAGC CAACGCAGTT CCTTGGAGAG

1651 AAGACATCAC AGTCCCTCTC AGAGGAGCCA CTGCAGTCCC TCTAGGAAAA

1701 ACCATTCCAG TCCTTCTGAG AGAAGCTGGC GCAGTCCGTC TCAGAGAAAT

1751 CACTGCAGTC CCCCCGAGAG GAGCTGTCAC AGTCTCTCTG AAAGGGGCCT

1801 TCACAGTCCC TCTCAGAGGA GCCATCGCGG TCCCTCTCAG AGAAGACATC

1851 ACAGTCCCTC AGAGAGAAGC CATCGCAGTC CCTCAGAGAG AAGCCATCGC

1901 AGTCCCTCTG AGAGAAGACA TCGCAGTCCC TCCCAGAGGA GCCATCGCGG

1951 TCCCTCAGAG AGAAGCCATT GCAGTCCCTC TGAGAGAAGA CATCGCAGTC

2001 CCTCTCAGAG GAGCCATCGT GGTCCCTCTG AGAGAAGACA TCACAGTCCC

2051 TCTAAGAGAA GCCATCGCAG TCCCGCTCGG AGGAGCCATC GCAGTCCCTC

2101 AGAGAGAAGC CATCACAGTC CCTCTGAGAG AAGCCATCAC AGTCCCTCTG

2151 AGAGAAGACA TCACAGTCCC TCTGAGAGAA GCCATTGCAG TCCCTCTGAG

2201 AGAAGCCATT GCAGTCCCTC TGAGAGAAGA CATCGCAGTC CCTCTGAGAG

2251 AAGACATCAC AGTCCCTCAG AGAAAAGCCA TCACAGTCCC TCTGAGAGAA

2301 GCCATCACAG TCCCTCTGAG AGAAGACGTC ACAGTCCCTT GGAGAGGAGC

2351 CGTCACAGTC TCTTGGAGAG GAGCCATCGC AGTCCCTCTG AGAGGAGATC

2401 TCACAGGTCC TTTGAGAGGA GCCATCGTAG GATTTCTGAG AGAAGTCACA

2451 GTCCCTCAGA GAAGAGCCAC CTCAGTCCCT TGGAAAGAAG CCGTTGCAGT

2501 CCCTCTGAGA GGAGAGGACA CAGTTCCTCT GGGAAAACCT GTCACAGTCC

2551 CTCTGAGAGA AGCCATCGCA GTCCCTCCGG GATGAGGCAA GGGAGGACCT

2601 CTGAGAGGAG CCATCGCAGT TCCTGTGAGA GAACCCGTCA CAGTCCCTCT 2651 GAGATGAGGC CAGGGAGGCC CTCTGGGAGG AACCATTGCA GTCCCTCTGA 2701 GAGGAGCCGA CGCAGTCCCC TTAAGGAGGG ACTCAAGTAC AGTTTCCCTG 2751 GAGAGAGGCC CAGCCATAGT TTGTCTAGAG ATTTCAAGAA TCAAACAACT 2801 CTCCTCGGGA CCACACATAA AAATCCCAAA GCAGGGCAAG TGTGGAGGCC 2851 TGAAGCTACT CGATGAGGCG AGGTCCGCCC CTATTATTCA TTGTCCTAAG 2901 TCTTCATCGT GCTGCCCTTT CCAGGCTTCT TTCCTGCTCA GCCACTGCCT 2951 CCAATTCCTG CGCCCCCAGC GTGGAAAGGC TTCCATTTCT CTCTACCGGG 3001 GGGGAGGCGG GTGAGAATGG GTCTGTAATT TCTCTAAGAT GAATAAAGGG 3051 GCAGTTAATT AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAGG

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 2

ORF from 47 bp to 2863 bp; peptide length: 939 Category: similarity to unknown protein Classification: unclassified Prosite motifs: ATP GTP A (824-832)

1 MEESEDSQSD SQTRISESQH SLKPNYLSQA KTDFSEQFQL LEDLQLKIAA 51 KLLRSQIPPD VPPPLASGLV LKYPICLQCG RCSGLNCHHK LQTTSGPYLL 101 IYPQLHLVRT PEGHGEVRLH LGFRLRIGKR SQISKYRERD RPVIRRSPIS 151 PSQRKAKIYT QASKSPTSTI DLQSGPSQSP APVQVYIRRG QRSRPDLVEK 201 TKTRAPGHYE FTQVHNLPES DSESTQNEKR AKVRTKKTSD SKYPMKRITK 251 RLRKHRKFYT NSRTTIESPS RELAAHLRRK RIGATQTSTA SLKRQPKKPS 301 QPKFMQLLFQ SLKRAFQTAH RVIASVGRKP VDGTRPDNLW ASKNYYPKQN 351 ARDYCLPSSI KRDKRSADKL TPAGSTIKQE DILWGGTVQC RSAQQPRRAY 401 SFQPRPLRLP KPTDSQSGIA FQTASVGQPL RTVQKDSSSR SKKNFYRNET 451 SSQESKNLST PGTRVQARGR ILPGSPVKRT WHRHLKDKLT HKEHNHPSFY 501 RERTPRGPSE RTRHNPSWRN HRSPSERSQR SSLERRHHSP SQRSHCSPSR 551 KNHSSPSERS WRSPSQRNHC SPPERSCHSL SERGLHSPSQ RSHRGPSQRR 601 HHSPSERSHR SPSERSHRSP SERRHRSPSQ RSHRGPSERS HCSPSERRHR 651 SPSQRSHRGP SERRHHSPSK RSHRSPARRS HRSPSERSHH SPSERSHHSP 701 SERRHHSPSE RSHCSPSERS HCSPSERRHR SPSERRHHSP SEKSHHSPSE 751 RSHHSPSERR RHSPLERSRH SLLERSHRSP SERRSHRSFE RSHRRISERS 801 HΞPSEKSHLS PLERSRCSPS ERRGHSSSGK TCHSPSERSH RSPSGMRQGR 851 TSERSHRSSC ERTRHSPSEM RPGRPSGRNH CSPSERSRRS PLKEGLKYSF 901 PGERPSHSLS RDFKNQTTLL GTTHKNPKAG QVWRPEATR

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_8gll, frame 2

TREMBL:AF061185_1 gene: "car90"; product: "cyst germination specific acidic repeat protein precursor"; Phytophthora infestans cyst germination specific acidic repeat protein precursor (car90) gene, complete eds., N = 1, Score = 457, P = 2.3e-39

TREMBL:AC004561_38 gene: "F16P2.41"; product: "putative proline-rich protein"; Arabidopsis thaliana chromosome II BAC F16P2 genomic sequence, complete sequence., N = 1, Score = 340, P = 4.2e-27

TREMBL:AF062655_1 product: "plenty-of-prolιnes-101"; Mus musculus plenty-of-prolιnes-101 mRNA, complete eds., N = 1, Score = 313, P = 3.6e-24

PIR:PN0099 son3 protein - human (fragment), N = 1, Score = 292, P = 1.2e-22

>TREMBL:AF061185_1 gene: "car90"; product: "cyst germination specific acidic repeat protein precursor"; Phytophthora infestans cyst germination specific acidic repeat protein precursor (car90) gene, complete eds. Length = 1,489

HSPs:

Score = 457 (68.6 bits), Expect = 2.3e-39, P = 2.3e-39 Identities = 91/444 (20%), Positives = 239/444 (53%)

Query: 475 SPVKRTWHRHLKDKLTHKEHNHPSFY-RERTPRGPSERTRHNPSWRNHRSPSERSQRSSL 533

+P + T + +++ T+ ++ E TP P+E T + P+ +P+E + +S Sbjct: 584 APTEETMYAPIEET-TYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAST 642

Query: 534 ERRHHSPSQRSHCSPSRKNHSSPSERSWRSPSQRNHCSPPERSCHSLSERGLHSPSQRSH 593

E ++P++ + +P+ + P+E + +P++ +P E + ++ +E ++P++ + Sbjct: 643 EETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETT 702

Query: 594 RGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRSHRGPSERSHCSPSERRHRSPS 653

P++ + P+E + +P+E + +P+E +P + + GP+E + +P+E +P+ Sbjct: 703 YAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPT 762

Query: 654 QRSHRGPSERRHHSPSKRSHRSPARRSHRSPSERSHHSPSERSHHSPSERRHHSPSERSH 713

+ + P+E + P+ + +P + +P+E + ++P+E + ++P+E + P+E + Sbjct: 763 EETPYAPTEETTYEPTGETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETT 822

Query: 714 CSPSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPSERRRHSPLERSRHSLL 773

+P+E + P+E +P+E ++P+E++ ++P+E++ ++P+E ++P E + + Sbjct: 823 YAPTEETPYEPTEETTYTPTEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPT 882

Query: 774 ERSHRSPSERRSHRSFERS-HRRISERSHSPSEKSHLSPLERSRCSPSERRGHSSSGKTC 832

E + +P++ ++ E + + E +++P+E++ +P E + P+E ++ + +T Sbjct: 883 EETTYAPTKETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETT 942

Query: 833 HSPSERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPSGRNHCSPSERΞRRSPL 892

++P+E + +P+ +E + + E T + P+E P+ +P+E + +P+ Sbjct: 943 YAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPI 1002

Query: 893 KEGLKYSFPGERPSHSLSRDFKNQTT 918

+E Y+ P E +++ + + + T Sbjct: 1003 EE-TTYA-PTEETTYAPAEETPYEPT 1026

Score = 445 (66.8 bits), Expect = 4.5e-38, P = 4.5e-38 Identities = 83/394 (21%), Positives = 212/394 (53%)

Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSW 561

E TP P+E T + P+ +P+E + + E ++P++ + +P+ + P+E + Sbjct: 763 EETPYAPTEETTYEPTGETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETT 822

Query: 562 RSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSPS 621

+P++ P E + ++ +E ++P++ + P+++ ++P+E + +P+E + P+ Sbjct: 823 YAPTEETPYEPTEETTYTPTEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPT 882

Query: 622 ERRHRΞPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681

E +P++ + P+E + + +E +P++ + P+E + P++ + +P + Sbjct: 883 EETTYAPTKETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETT 942

Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPS 741

+P+E + ++P+E + ++P+E ++P+E + P+E + +P+E +P+E ++P Sbjct: 943 YAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPI 1002

Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERS-HRRISERS 800

E++ ++P+E + ++P+E + P E + ++ E + +P+E ++ S E + + E + Sbjct: 1003 EETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYASTEETTYAPTEETT 1062

Query: 801 HSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSC 860

++P+E++ P E + +P+E ++ + +T ++P+E + +P+ +E + Sbjct: 1063 YAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPT 1122

Query: 861 ERTRHSPSEMRPGRPSGRNHCSPSERSRRSPLKE 894

E T ++P+E P+ +P E + P +E Sbjct: 1123 EETTYAPTEETTYAPTEETMYAPIEETTYGPTEE 1156

Score = 439 (65.9 bits), Expect = 2.0e-37, P = 2.0e-37 Identities = 86/421 (20%), Positives = 223/421 (52%)

Query: 475 SPVKRTWHRHLKDKLTHKEHNHPSFY-RERTPRGPΞERTRHNPSWRNHRSPSERSQRSSL 533

+P + T + +K T+ ++ E TP P+E T + P+ +P+E + +S Sbjct: 848 APTEETTYAPT-EKTTYAPTEETTYAPTEETPYEPTEETTYAPTKETTYAPTEETTYAST 906

Query: 534 ERRHHSPSQRSHCSPSRKNHSSPSERSWRSPSQRNHCSPPERSCHΞLSERGLHSPSQRSH 593

E ++P++ + +P+ + P+E + +P++ +P E + ++ +E ++P++ + Sbjct: 907 EETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETT 966 Query: 594 RGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRSHRGPSERSHCSPSERRHRSPS 653

P++ + P+E + +P+E + +P+E +P + + P+E + +P+E P+ Sbjct: 967 YAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYAPTEETTYAPAEETPYEPT 1026

Query: 654 QRSHRGPSERRHHSPSKRSHRSPARRSHRSPSERSHHSPSERSHHSPSERRHHSPSERSH 713

+ + P+E ++P++ + + + +P+E + ++P+E + + P+E ++P+E + Sbjct: 1027 EETTYAPTEETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETT 1086

Query: 714 CSPSERSHCSPSERRHRΞPSERRHHSPSEKSHHSPSERΞHHSPSERRRHSPLERSRHSLL 773

+P+E + +P+E +P+E ++P+E++ + P+E + ++P+E ++P E + ++ + Sbjct: 1087 YAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPI 1146

Query: 774 ERSHRSPSERRSHRSFERS-HRRISERSHSPSEKSHLSPLERSRCSPSERRGHSSSGKTC 832

E + P+E ++ E + + E ++P+E++ P + +P+E ++ + +T Sbjct: 1147 EETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPTGETTYAPTEETTYAPTEETT 1206

Query: 833 HSPSERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPSGRNHCSPSERSRRSPL 892

++P+E + +P+ +E + + E T + P+E P+ +P+E + +P Sbjct: 1207 YAPTEETTYAPTEETPYEPTEETTYAPTEETTYEPTEETTYAPTEETTYAPTEETTYAPT 1266

Query: 893 KE 894

+E Sbjct: 1267 EE 1268

Score = 439 (65.9 bits), Expect = 2.0e-37, P = 2.0e-37 Identities = 91/434 (20%), Positives = 232/434 (53%)

Query: 475 SPVKRTWHRHLKDKLTHKEHNHPSFY-RERTPRGPSERTRHNPSWRNHRSPSERSQRSSL 533

+P + T + +K T+ ++ E TP P+E T + P+ +P+E + +S Sbjct: 440 APTEETTYAPT-EKTTYAPTEETTYAPTEETPYEPTEETTYAPTKETTYAPTEETTYAST 498

Query: 534 ERRHHSPSQRSHCΞPSRKNHSSPSERSWRSPSQRNHCSPPERSCHSLΞERGLHSPSQRSH 593

E ++P++ + +P+ + P+E + +P++ +P E + ++ +E ++P++ + Sbjct: 499 EETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETT 558

Query: 594 RGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRSHRGPSERSHCSPSERRHRSPS 653

P++ + P+E + +P+E + +P+E +P + + P+E + +P+E P+ Sbjct: 559 YAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYAPTEETTYAPAEETPYEPT 618

Query: 654 QRSHRGPSERRHHSPSKRSHRSPARRSHRSPSERSHHSPSERSHHSPSERRHHSPSERSH 713

+ + P+E ++P++ + + + +P+E + ++P+E + + P+E ++P+E + Sbjct: 619 EETTYAPTEETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETT 678

Query: 714 CSPSERSHCΞPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPSERRRHΞPLERSRHSLL 773

+P+E + +P+E +P+E ++P+E++ + P+E + ++P+E ++P E + ++ + Sbjct: 679 YAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPI 738

Query: 774 ERSHRSPSERRSHRΞFERS-HRRISERSHSPSEKSHLSPLERSRCSPSERRGHSSSGKTC 832

E + P+E ++ E + + E ++P+E++ P + +P+E ++ + +T Sbjct: 739 EETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPTGETTYAPTEETTYAPTEETT 798

Query: 833 HSPSERSHRSPSGMRQGRTSERΞHRSSCERTRHSPSEMRPGRPSGRNHCSPSERSRRSPL 892

++P+E + +P T E + + E T ++P+E P P+ +P+E + +P Sbjct: 799 YAPTEETTYAP TEETPYEPT-EETTYAPTEETPYEPTEETTYTPTEETTYAPT 850

Query: 893 KEGLKYSFPGERPSHS 908

+E Y+ P E+ +++ Sbjct: 851 EE-TTYA-PTEKTTYA 864

Score = 437 (65.6 bits), Expect = 3.3e-37, P = 3.3e-37 Identities = 85/417 (20%), Positives = 223/417 (53%)

Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSW 561

E TP P+E T + P+ +P+E + + E+ ++P++ + +P+ + P+E + Sbjct: 419 EETPYEPTEETTYTPTEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPTEETT 478

Query: 562 RSPSQRNHCSPPERSCHSLSERGLHSPSQRΞHRGPSQRRHHSPSERSHRSPSERSHRSPS 621

+P++ +P E + ++ +E ++P++ + P++ + P+E + +P+E + +P+ Sbjct: 479 YAPTKETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPT 538

Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681

E +P++ + P+E + +P+E P++ + P+E ++P++ + +P + Sbjct: 539 EETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETT 598

Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPS 741

+P+E + ++P+E + + P+E ++P+E + +P+E + + +E +P+E ++P+ Sbjct: 599 YAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYASTEETTYAPTEETTYAPA 658

Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERS-HRRISERS 800

E++ + P+E + ++P+E ++P E + ++ E + +P+E ++ E + + E + Sb_jct: 659 EETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETT 718 Query: 801 HSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSC 860

++P+E++ +P E + +P E + + +T ++P+E + +P+ +E + Sbjct: 719 YAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPT 778

Query: 861 ERTRHSPΞEMRPGRPSGRNHCSPSERSRRSPLKEGLKYSFPGERPSHSLSRDFKNQTT 918

T ++P+E P+ +P+E + +P +E Y P E +++ + + + T Sbjct: 779 GETTYAPTEETTYAPTEETTYAPTEETTYAPTEE-TPYE-PTEETTYAPTEETPYEPT 834

Score = 428 (64.2 bits), Expect = 3. le-36, P = 3. le-36 Identities = 89/440 (20%), Positives = 228/440 (51%)

Query: 473 PGSPVKRTWHRHLKDKLTHKEHNHPSFYR-ERTPRGPSERTRHNPSWRNHRSPSERSQRS 531

P P + T + K+ T+ ++ E T P+E T + P+ P+E + + Sbjct: 470 PYEPTEETTYAPTKET-TYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYA 528

Query: 532 SLERRHHSPSQRSHCSPSRKNHSSPSERSWRSPSQRNHCSPPERSCHSLSERGLHSPSQR 591

E ++P++ + +P+ + +P+E + +P++ P E + ++ +E ++P++ Sbjct: 529 PTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEE 588

Query: 592 SHRGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRSHRGPSERΞHCSPSERRHRS 651

+ P + ++P+E + +P+E + P+E +P++ + P+E + + +E + Sbjct: 589 TMYAPIEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYASTEETTYA 648

Query: 652 PSQRSHRGPSERRHHΞPSKRSHRSPARRSHRSPSERSHHSPSERSHHSPSERRHHSPSER 711

P++ + P+E + P++ + +P + +P+E + ++P+E + ++P+E ++P+E Sbjct: 649 PTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEE 708

Query: 712 SHCSPSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPΞERRRHSPLERSRHS 771

+ P+E + +P+E +P+E ++P E++ + P+E + ++P+E ++P E + ++ Sbjct: 709 TPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPTEETPYA 768

Query: 772 LLERSHRSPSERRΞHRSFERS-HRRISERSHSPSEKSHLSPLERSRCSPSERRGHSSSGK 830

E + P+ ++ E + + E +++P+E++ +P E + P+E ++ + + Sbjct: 769 PTEETTYEPTGETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYAPTEE 828

Query: 831 TCHSPSERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPSGRNHCSPSERSRRS 890

T + P+E + +P+ +E + + E+T ++P+E P+ P+E + + Sbjct: 829 TPYEPTEETTYTPTEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPTEETTYA 888

Query: 891 PLKEGLKYSFPGERPSHSLSRD 912

P KE Y+ P E +++ + + Sbjct: 889 PTKE-TTYA-PTEETTYASTEE 908

Score = 427 (64.1 bits), Expect = 4.0e-36, P = 4.0e-36 Identities = 81/394 (20%), Positives = 213/394 (54%)

Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRΞHCSPSRKNHSSPSERSW 561

E T GP+E T + P+ +P+E + + E + P+ + +P+ + +P+E + Sbjct: 739 EETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPTGETTYAPTEETTYAPTEETT 798

Query: 562 RSPSQRNHCSPPERSCHSLSERGLHΞPSQRSHRGPSQRRHHΞPSERSHRSPSERSHRSPS 621

+P++ +P E + + +E ++P++ + P++ ++P+E + +P+E + +P+ Sbjct: 799 YAPTEETTYAPTEETPYEPTEETTYAPTEETPYEPTEETTYTPTEETTYAPTEETTYAPT 858

Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681

E+ +P++ + P+E + P+E +P++ + P+E ++ ++ + +P + Sbjct: 859 EKTTYAPTEETTYAPTEETPYEPTEETTYAPTKETTYAPTEETTYASTEETTYAPTEETT 918

Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERΞHCSPSERRHRSPSERRHHSPS 741

+P+E + + P+E + ++P+E ++P+E + +P+E + +P+E +P+E + P+ Sbjct: 919 YAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPT 978

Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRΞPSERRSHRSFERS-HRRISERS 800

E++ ++P+E + ++P+E ++P+E + ++ E + +P+E + E + + E + Sbjct: 979 EETTYAPTEETTYAPTEETMYAPIEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETT 1038

Query: 801 HSPSEKSHLSPLERΞRCSPSERRGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSC 860

++P+E++ + E + +P+E ++ + +T + P+E + +P+ +E + + Sbjct: 1039 YAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPT 1098

Query: 861 ERTRHSPSEMRPGRPSGRNHCSPSERSRRSPLKE 894

E T ++P+E P+ P+E + +P +E Sbjct: 1099 EETTYAPTEETTYAPAEETPYEPTEETTYAPTEE 1132

Score = 424 (63.6 bits), Expect = 8.5e-36, P = 8.5e-36 Identities = 81/394 (20%), Positives = 210/394 (53%)

Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSW 561

E T P+E T + P+ +P+E + + E + P++ + +P+ + +P+E + Sb_jct: 939 EETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETM 998 Query: 562 RSPSQRNHCSPPERΞCHSLSERGLHSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSPS 621

+P + +P E + ++ +E + P++ + P++ ++P+E + + +E + +P+ Sbjct: 999 YAPIEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYASTEETTYAPT 1058

Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681

E +P++ + P+E + +P+E +P++ + P+E ++P++ + +PA + Sbjct: 1059 EETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETP 1118

Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPS 741

P+E + ++P+E + ++P+E ++P E + P+E + +P+E +P+E ++P+ Sbjct: 1119 YEPTEETTYAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPTEETPYAPT 1178

Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERS-HRRISERS 800

E++ + P+ + ++P+E ++P E + ++ E + +P+E + E + + E + Sbjct: 1179 EETTYEPTGETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYAPTEETT 1238

Query: 801 HSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSC 860

+ P+E++ +P E + +P+E ++ + +T ++P + + P+ +E + + Sbjct: 1239 YEPTEETTYAPTEETTYAPTEETTYAPTEETMYAPIDETTYGPTEETTYAPTEATTYAPT 1298

Query: 861 ERTRHSPSEMRPGRPSGRNHCSPSERSRRSPLKE 894

E T ++P+E P+G +P+E + +P +E Sbjct: 1299 EETPYAPTEETTYEPTGETTYAPTEETTYAPTEE 1332

Score = 422 (63.3 bits). Expect = 1.4e-35, P = 1.4e-35 Identities = 84/407 (20%), Positives = 216/407 (53%)

Query: 502 ERTPRGPSERTRHNPSWRNHRΞPSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSW 561

E T P+E T + P+ P+E + + E + P++ + +P+ + +P+E + Sbjct: 795 EETTYAPTEETTYAPTEETPYEPTEETTYAPTEETPYEPTEETTYTPTEETTYAPTEETT 854

Query: 562 RSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPΞERSHRSPSERSHRSPS 621

+P+++ +P E + ++ +E + P++ + P++ ++P+E + + +E + +P+ Sbjct: 855 YAPTEKTTYAPTEETTYAPTEETPYEPTEETTYAPTKETTYAPTEETTYASTEETTYAPT 914

Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681

E +P++ + P+E + +P+E +P++ + P+E ++P++ + +PA + Sbjct: 915 EETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETP 974

Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPS 741

P+E + ++P+E + ++P+E ++P E + +P+E + +P+E P+E ++P+ Sbjct: 975 YEPTEETTYAPTEETTYAPTEETMYAPIEETTYAPTEETTYAPAEETPYEPTEETTYAPT 1034

Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRΞHRSFERS-HRRISERS 800

E++ ++P+E + ++ +E ++P E + ++ E + P+E ++ E + + E + Sbjct: 1035 EETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETT 1094

Query: 801 HSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSC 860

++P+E++ +P E + +P+E + + +T ++P+E + +P+ E + Sbjct: 1095 YAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYGPT 1154

Query: 861 ERTRHSPSEMRPGRPSGRNHCSPSERSRRSPLKEGLKYSFPGERPΞHS 908

E T ++P+E P+ +P+E + P E Y+ P E +++ Sbjct: 1155 EETTYAPTEATTYAPTEETPYAPTEETTYEPTGE-TTYA-PTEETTYA 1200

Score = 421 (63.2 bits), Expect = 1.8e-35, P = 1.8e-35 Identities = 86/418 (20%), Positives = 219/418 (52%)

Query: 491 HKEHNHPSFYRERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSR 550

H H E T P+E T + P+ +P+E + + E + P++ + +P+ Sbjct: 376 HYAHIEKPCDTEVTMYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYTPTE 435

Query: 551 KNHSSPSERSWRSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPSERSHR 610

+ +P+E + +P+++ +P E + ++ +E + P++ + P++ ++P+E + Sbjct: 436 ETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPTEETTYAPTKETTYAPTEETTY 495

Query: 611 SPSERSHRSPSERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSK 670

+ +E + +P+E +P++ + P+E + +P+E +P++ + P+E ++P++ Sbjct: 496 ASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTE 555

Query: 671 RSHRSPARRSHRSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHR 730

+ +PA + P+E + ++P+E + ++P+E ++P E + +P+E + +P+E Sbjct: 556 ETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYAPTEETTYAPAEETPY 615

Query: 731 SPSERRHHSPSEKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFE 790

P+E ++P+E++ ++P+E + ++ +E ++P E + ++ E + P+E ++ E Sbjct: 616 EPTEETTYAPTEETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTE 675

Query: 791 RS-HRRISERSHSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPSGMRQG 849 + + E +++P+E++ +P E + +P+E + + +T ++P+E + +P+ Sbjct: 676 ETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMY 735

Query: 850 RTSERSHRSSCERTRHSPSEMRPGRPSGRNHCSPSERSRRSPLKEGLKYSFPGERPΞHS 908

E + E T ++P+E P+ +P+E + P E Y+ P E +++ Sbjct: 736 APIEETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPTGE-TTYA-PTEETTYA 792

Score = 420 (63.0 bits), Expect = 2.3e-35, P = 2.3e-35 Identities = 82/393 (20%), Positives = 206/393 (52%)

Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSW 561

E TP P+E T + P+ +P+E + + +E ++P++ + +P+ + P+E + Sbjct: 971 EETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYAPTEETTYAPAEETPYEPTEETT 1030

Query: 562 RSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSPS 621

+P++ +P E + ++ +E ++P++ + P++ + P+E + +P+E + +P+ Sbjct: 1031 YAPTEETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPT 1090

Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681

E +P++ + P+E + +P+E P++ + P+E ++P++ + +P + Sbjct: 1091 EETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETT 1150

Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPS 741

P+E + ++P+E + ++P+E ++P+E + P+ + +P+E +P+E ++P+ Sbjct: 1151 YGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPTGETTYAPTEETTYAPTEETTYAPT 1210

Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERS-HRRISERS 800

E++ ++P+E + + P+E ++P E + + E + +P+E ++ E + + E Sbjct: 1211 EETTYAPTEETPYEPTEETTYAPTEETTYEPTEETTYAPTEETTYAPTEETTYAPTEETM 1270

Query: 801 HSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPΞGMRQGRTSERΞHRSSC 860

++P +++ P E + +P+E ++ + +T ++P+E + P+G +E + + Sbjct: 1271 YAPIDETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPTGETTYAPTEETTYAPT 1330

Query: 861 ERTRHSPSEMRPGRP SGRNHCSPSE 885

E T ++P E P P S C+ E Sbjct: 1331 EETTYAPMEETPYEPAEESTSTVSTEKPCNTEE 1363

Score = 419 (62.9 bits), Expect = 3.0e-35, P = 3.0e-35 Identities = 83/411 (20%), Positives = 215/411 (52%)

Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSW 561

E T P+E T + P+ +P+E + E ++P++ + +P+ + +P E + Sbjct: 947 EETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETT 1006

Query: 562 RSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPΞERSHRSPSERSHRSPS 621

+P++ +P E + + +E ++P++ + P++ ++ +E + +P+E + +P+ Sbjct: 1007 YAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYASTEETTYAPTEETTYAPA 1066

Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681

E P++ + P+E + +P+E +P++ + P+E ++P++ + P + Sbjct: 1067 EETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETT 1126

Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERΞHCSPSERRHRSPSERRHHSPΞ 741

+P+E + ++P+E + ++P E + P+E + +P+E + +P+E +P+E + P+ Sbjct: 1127 YAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPT 1186

Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERS-HRRISERS 800

++ ++P+E + ++P+E ++P E + ++ E + P+E ++ E + + E + Sbjct: 1187 GETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYAPTEETTYEPTEETT 1246

Query: 801 HSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSC 860

++P+E++ +P E + +P+E ++ +T + P+E + +P+ +E + + Sbjct: 1247 YAPTEETTYAPTEETTYAPTEETMYAPIDETTYGPTEETTYAPTEATTYAPTEETPYAPT 1306

Query: 861 ERTRHSPSEMRPGRPSGRNHCSPSERSRRSPLKEGLKYSFPGERPSHSLSRD 912

E T + P+ P+ +P+E + +P++E Y P E + ++S + Sbjct: 1307 EETTYEPTGETTYAPTEETTYAPTEETTYAPMEE-TPYE-PAEESTSTVΞTE 1356

Score = 415 (62.3 bits), Expect = 8.0e-35, P = 8.0e-35 Identities = 84/423 (19%), Positives = 218/423 (51%)

Query: 473 PGSPVKRTWHRHLKDKLTHKEHNHPSFYR-ERTPRGPSERTRHNPSWRNHRSPSERSQRS 531

P P + T + K+ T+ ++ E T P+E T + P+ P+E + + Sbjct: 878 PYEPTEETTYAPTKET-TYAPTEETTYAΞTEETTYAPTEETTYAPAEETPYEPTEETTYA 936

Query: 532 SLERRHHSPSQRSHCSPSRKNHSSPSERSWRSPSQRNHCSPPERSCHSLSERGLHSPSQR 591

E ++P++ + +P+ + +P+E + +P++ P E + ++ +E ++P++ Sbjct: 937 PTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEE 996

Query: 592 SHRGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRSHRGPSERSHCSPSERRHRS 651 + P + ++P+E + +P+E + P+E +P++ + P+E + + +E + Sbjct: 997 TMYAPIEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYASTEETTYA 1056

Query: 652 PSQRSHRGPSERRHHSPSKRSHRSPARRSHRSPSERSHHSPSERSHHSPSERRHHSPSER 711

P++ + P+E + P++ + +P + +P+E + ++P+E + ++P+E ++P+E Sbjct: 1057 PTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEE 1116

Query: 712 SHCSPSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPSERRRHSPLERSRHS 771

+ P+E + +P+E +P+E ++P E++ + P+E + ++P+E ++P E + ++ Sbjct: 1117 TPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPTEETPYA 1176

Query: 772 LLERSHRSPSERRSHRSFERS-HRRIΞERSHSPSEKSHLSPLERSRCSPSERRGHSSSGK 830

E + P+ ++ E + + E +++P+E++ +P E + P+E ++ + + Sbjct: 1177 PTEETTYEPTGETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYAPTEE 1236

Query: 831 TCHSPSERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPΞGRNHCSPSERSRRS 890

T + P+E + +P+ +E + + E T ++P + P+ +P+E + + Sbjct: 1237 TTYEPTEETTYAPTEETTYAPTEETTYAPTEETMYAPIDETTYGPTEETTYAPTEATTYA 1296

Query: 891 PLKE 894

P +E Sbjct: 1297 PTEE 1300

Score = 403 (60.5 bits), Expect = 1.6e-33, P = 1.6e-33 Identities = 84/394 (21%), Positives = 213/394 (54%)

Query: 501 RERTPRGPSERTRHNPSWRNHRSPSERΞQRSSLERRHHSPSQRSHCSPSRKNHSSPSERS 560

RE T PSE T + P +P+E+ +E + + ++ +P++ ++P+ER Sbjct: 319 REETTAAPSEDTTYAPREVTPYAPTEKPY—DVEETTYVTEESTY-APTKSETNAPTERM 375

Query: 561 WRSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSP 620

+ ++ C E + ++ +E ++P++ + P++ ++P+E + P+E + +P Sbjct: 376 HYAHIEKP-CDT-EVTMYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYTP 433

Query: 621 SERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRS 680

+E +P++ + P+E++ +P+E +P++ + P+E ++P+K + +P + Sbjct: 434 TEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPTEETTYAPTKETTYAPTEET 493

Query: 681 HRSPSERSHHSPSERSHHSPΞERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSP 740

+ +E + ++P+E + ++P+E + P+E + +P+E + +P+E +P+E ++P Sbjct: 494 TYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAP 553

Query: 741 SEKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERS-HRRISER 799

+E++ ++P+E + + P+E ++P E + ++ E + +P E ++ E + + E Sbjct: 554 TEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYAPTEETTYAPAEET 613

Query: 800 SHSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPΞGMRQGRTSERSHRSS 859

+ P+E++ +P E + +P+E ++S+ +T ++P+E + +P+ +E + + Sbjct: 614 PYEPTEETTYAPTEETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAP 673

Query: 860 CERTRHSPSEMRPGRPSGRNHCSPSERSRRSPLKE 894

E T ++P+E P+ +P+E + +P +E Sbjct: 674 TEETTYAPTEETTYAPTEETTYAPTEETTYAPAEE 708

Score = 398 (59.7 bits), Expect = 5.5e-33, P = 5.5e-33 Identities = 84/402 (20%), Positives = 209/402 (51%)

Query: 475 SPVKRTWHRHLKDKLTHKEHNHPSFY-RERTPRGPSERTRHNPSWRNHRSPSERSQRSSL 533

+P + T + +++ T+ ++ E TP P+E T + P+ +P+E + +S Sbjct: 992 APTEETMYAPIEET-TYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAST 1050

Query: 534 ERRHHSPSQRSHCSPSRKNHSSPSERSWRSPSQRNHCSPPERSCHSLSERGLHSPSQRSH 593

E ++P++ + +P+ + P+E + +P++ +P E + ++ +E ++P++ + Sbjct: 1051 EETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETT 1110

Query: 594 RGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRSHRGPΞERSHCSPSERRHRSPS 653

P++ + P+E + +P+E + +P+E +P + + GP+E + +P+E +P+ Sbjct: llll YAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPT 1170

Query: 654 QRSHRGPSERRHHSPSKRSHRSPARRSHRSPΞERSHHSPSERSHHSPSERRHHSPSERSH 713

+ + P+E + P+ + +P + +P+E + ++P+E + ++P+E + P+E + Sbjct: 1171 EETPYAPTEETTYEPTGETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETT 1230

Query: 714 CSPSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPSERRRHSPLERSRHSLL 773

+P+E + P+E +P+E ++P+E++ ++P+E + ++P + + P E + ++ Sbjct: 1231 YAPTEETTYEPTEETTYAPTEETTYAPTEETTYAPTEETMYAPIDETTYGPTEETTYAPT 1290

Query: 774 ERSHRSPSERRSHRSFERSHRRISERSHSPSEKΞHLSPLERSRCSPSERRGHSSSGKTCH 833

E + +P+E + E E ++ P+ ++ +P E + +P+E ++ +T + Sbjct: 1291 EATTYAPTEETPYAPTE ETTYEPTGETTYAPTEETTYAPTEETTYAPMEETPY 1343

Query: 834 SPΞERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPS 876 P+E S + S + T E + + E T PS+ P+ Sb_jct: 1344 EPAEESTSTVSTEKPCNTEEFTDEPTDEPT-DEPSDEPTDEPT 1385

Score = 368 (55.2 bits), Expect = 9.5e-30, P = 9.5e-30 Identities = 79/386 (20%), Positives = 211/386 (54%)

Query: 524 PSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSWRSPSQRNHCSPPERSCHSLSER 583

PS+ ++ + E + P + + +PS +P E + +P+++ + E + + ++E Sb_jct: 303 PSDETEAPT-EGTTYVPREETTAAPSEDTTYAPREVTPYAPTEKPY—DVEETTY-VTEE 358

Query: 584 GLHSPSQRSHRGPSQRRHHSPΞER SHRSPSERSHRSPSERRHRSPΞQRSHRGPS 637

++P++ P++R H++ E+ + +P+E + +P+E +P++ + P+ Sbjct: 359 STYAPTKSETNAPTERMHYAHIEKPCDTEVTMYAPTEETTYAPTEETTYAPTEETTYAPT 418

Query: 638 ERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSHRSPSERSHHSPSERSH 697

E + P+E +P++ + P+E ++P++++ +P + +P+E + + P+E + Sbjct: 419 EETPYEPTEETTYTPTEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPTEETT 478

Query: 698 HSPSERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPS 757

++P++ ++P+E + + +E + +P+E +P+E + P+E++ ++P+E + ++P+ Sbjct: 479 YAPTKETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPT 538

Query: 758 ERRRHSPLERSRHSLLERSHRSPSERRSHRSFERS-HRRISERSHSPSEKSHLSPLERSR 816

E ++P E + ++ E + +P+E + E + + E +++P+E++ +P+E + Sbjct: 539 EETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETT 598

Query: 817 CSPSERRGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRΞSCERTRHSPSEMRPGRPS 876

+P+E ++ + +T + P+E + +P+ +E + +S E T ++P+E P+ Sbjct: 599 YAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYASTEETTYAPTEETTYAPA 658

Query: 877 GRNHCSPSERSRRSPLKEGLKYSFPGERPSHS 908

P+E + +P +E Y+ P E +++ Sbjct: 659 EETPYEPTEETTYAPTEE-TTYA-PTEETTYA 688

Score = 337 (50.6 bits), Expect = 2. le-26, P = 2. le-26 Identities = 66/328 (20%), Positives = 170/328 (51%)

Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSW 561

E T P+E T + P+ +P+E + + E ++P++ + +P+ + +P+E + Sbjct: 1059 EETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETP 1118

Query: 562 RSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSPS 621

P++ +P E + ++ +E +++P + + GP++ ++P+E + +P+E + +P+ Sbjct: 1119 YEPTEETTYAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPTEETPYAPT 1178

Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681

E P+ + P+E + +P+E +P++ + P+E + P++ + +P + Sbjct: 1179 EETTYEPTGETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYAPTEETT 1238

Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHRΞPSERRHHSPS 741

P+E + ++P+E + ++P+E ++P+E + +P + + P+E +P+E ++P+ Sbjct: 1239 YEPTEETTYAPTEETTYAPTEETTYAPTEETMYAPIDETTYGPTEETTYAPTEATTYAPT 1298

Query: 742 EKSHHSPΞERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERSHRRIS 797

E++ ++P+E + + P+ ++P E + ++ E + +P E + E S +S Sbjct: 1299 EETPYAPTEETTYEPTGETTYAPTEETTYAPTEETTYAPMEETPYEPAEESTSTVSTEKP 1358

Query: 798 ERSHSPSEKSHLSPLERSRCSPSE 821

E + P+++ P + P++ Sbjct: 1359 CNTEEFTDEPTDEPTDEPSDEPTDEPTD 1386

Score = 333 (50.0 bits), Expect = 5.7e-26, P = 5.7e-26 Identities = 63/320 (19%), Positives = 166/320 (51%)

Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSRKNHΞSPSERSW 561

E T P+E T + P+ +P+E + + E ++P++ + P+ + +P+E + Sbjct: 1075 EETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETT 1134

Query: 562 RSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSPS 621

+P++ +P E + + +E ++P++ + P++ ++P+E + P+ + +P+ Sbjct: 1135 YAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPTGETTYAPT 1194

Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPΞKRSHRSPARRSH 681

E +P++ + P+E + +P+E P++ + P+E + P++ + +P + Sbjct: 1195 EETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYAPTEETTYEPTEETTYAPTEETT 1254

Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPΞ 741

+P+E + ++P+E + ++P + + P+E + +P+E + +P+E +P+E + P+ Sbjct: 1255 YAPTEETTYAPTEETMYAPIDETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPT 1314

Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERSHRRISERSH 801 ++ ++P+E + ++P+E ++P+E + + E S + S + + E + E + Sbjct: 1315 GETTYAPTEETTYAPTEETTYAPMEETPYEPAEESTSTVSTEKPCNTEEFTDEPTDEPTD 1374

Query: 802 SPSEKSHLSPLERSRCSPSE 821

PS++ P + P++ Sbjct: 1375 EPSDEPTDEPTDEPTDLPTD 1394

Score = 303 (45.5 bits), Expect = 9.6e-23, P = 9.6e-23 Identities = 70/322 (21%), Positives = 170/322 (52%)

Query: 584 GLHSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRΞHRGPSERSHCS 643

G + PS + P++ + P E + +PSE + +P E +P+++ + E ++ + Sbjct: 299 GGYEPΞDETE-APTEGTTYVPREETTAAPSEDTTYAPREVTPYAPTEKPY-DVEETTYVT 356

Query: 644 PSERRHRSPSQRSHRGPSERRHHSPSKRSHRΞPARRSHRSPSERSHHSPSERSHHSPSER 703

E +P++ P+ER H++ ++ + + +P+E + ++P+E + ++P+E Sbjct: 357 --EESTYAPTKSETNAPTERMHYAHIEKPCDTEV— MYAPTEETTYAPTEETTYAPTEE 412

Query: 704 RHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPSERRRHS 763

++P+E + P+E + +P+E +P+E ++P+EK+ ++P+E + ++P+E + Sbjct: 413 TTYAPTEETPYEPTEETTYTPTEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYE 472

Query: 764 PLERSRHSLLERSHRSPSERRSHRSFERS-HRRISERSHSPSEKSHLSPLERSRCSPSER 822

P E + ++ + + +P+E ++ S E + + E +++P+E++ P E + +P+E Sbjct: 473 PTEETTYAPTKETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEE 532

Query: 823 RGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPSGRNHCΞ 882

++ + +T ++P+E + +P+ +E + E T ++P+E P+ + Sbjct: 533 TTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYA 592

Query: 883 PSERSRRSPLKEGLKYSFPGERP 905

P E + +P +E Y+ E P Sbjct: 593 PIEETTYAPTEE-TTYAPAEETP 614

Score = 151 (22.7 bits), Expect = 2.0e-06, P = 2.0e-06 Identities = 45/198 (22%), Positives = 103/198 (52%)

Query: 716 PSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPSERRRHSPLERSRHSLLER 775

PS+ + +P+E P E +PSE + ++P E + ++P+E+ +E + + + E Sbjct: 303 PSDETE-APTEGTTYVPREETTAAPSEDTTYAPREVTPYAPTEKPYD--VEETTY-VTEE 358

Query: 776 SHRSPSERRSHRSFERSHRRISERS HSPSEKSHLSPLERSRCSPSERRGHSSS 828

S +P++ ++ ER H E+ ++P+E++ +P E + +P+E ++ + Sbjct: 359 STYAPTKSETNAPTERMHYAHIEKPCDTEVTMYAPTEETTYAPTEETTYAPTEETTYAPT 418

Query: 829 GKTCHSPSERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPSGRNHCSPSERSR 888

+T + P+E + +P+ +E + + E+T ++P+E P+ P+E + Sbjct: 419 EETPYEPTEETTYTPTEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPTEETT 478

Query: 889 RSPLKEGLKYSFPGERPSHSLSRD 912

+P KE Y+ P E +++ + + Sbjct: 479 YAPTKE-TTYA-PTEETTYASTEE 500

Pedant information for DKFZphtes3_8gll, frame 2

Report for DKFZphtes3_8gll .2

[LENGTH] 954

[MW] 110063.05

[pi] 11.40

[PROSITE] ATP_GTP_A 1

[KW] Irregular

[KW] LOW_COMPLEXITY 27.67 %

SEQ ESSLSIFYDREDLVPMEESEDSQSDSQTRISESQHSLKPNYLSQAKTDFSEQFQLLEDLQ SEG xxxxxxxxxxx

PRD ccceeeccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhh

SEQ LKIAAKLLRSQIPPDVPPPLASGLVLKYPICLQCGRCSGLNCHHKLQTTSGPYLLIYPQL SEG

PRD hhhhhhhhhhcccccccccccceeeeecceeecccccccccccccccccccceeeehhhh

SEQ HLVRTPEGHGEVRLHLGFRLRIGKRSQISKYRERDRPVIRRSPISPSQRKAKIYTQASKS SEG

PRD hcccccccccceeecccceeeccccccccccccccceeeeeccccccchhhhhhhccccc

SEQ PTSTIDLQSGPSQSPAPVQVYIRRGQRSRPDLVEKTKTRAPGHYEFTQVHNLPESDSEST SEG

PRD ccccccccccccccccceeeeeeeccccccchhhhhhcccccceeeeeecccccccccch

SEQ QNEKRAKVRTKKTSDSKYPMKRITKRLRKHRKFYTNSRTTIESPSRELAAHLRRKRIGAT

SEG

PRD hhhhhhhhhhccccccccccchhhhhhhhhhhccccccccccccchhhhhhhhhhhhhcc

SEQ QTSTASLKRQPKKPSQPKFMQLLFQSLKRAFQTAHRVIASVGRKPVDGTRPDNLWASKNY

SEG

PRD ccchhhhhccccccccchhhhhhhhhhhhhhhhhhhhhhccccccccccccccccccccc

SEQ YPKQNARDYCLPSSIKRDKRSADKLTPAGSTIKQEDILWGGTVQCRSAQQPRRAYSFQPR

SEG

PRD cccccccccccccccccccccccccccccccccccceeeccccccccccccccccccccc

SEQ PLRLPKPTDSQSGIAFQTASVGQPLRTVQKDSSSRSKKNFYRNETSSQESKNLΞTPGTRV

SEG

PRD ccccccccccccceeeecccccccceeeeeccccccccccccccccccccccccccccee

SEQ QARGRILPGSPVKRTWHRHLKDKLTHKEHNHPΞFYRERTPRGPSERTRHNPSWRNHRSPS

SEG xxxxx

PRD eeecccccccccccccccccccccccccccccceeeeccccccccccccccccccccccc

SEQ ERSQRSSLERRHHSPSQRSHCSPSRKNHSΞPSERSWRSPSQRNHCSPPERΞCHSLSERGL

SEG xxxxxxxxxxxxxx xxxxxxxxxxxx

PRD chhhhhhhhhhccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ HSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRSHRGPSERSHCSPS

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ ERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSHRSPSERSHHSPSERSHHSPSERRH

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ HSPΞERSHCSPSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPSERRRHSPL

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ ERSRHSLLERSHRSPSERRSHRSFERSHRRISERSHSPSEKSHLSPLERSRCSPSERRGH

SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

PRD hhhhhhhhhhhccccccccchhhhhhhhhhhhhccccccccccccccccccccccccccc

SEQ SSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPSGRNHCSPSE

SEG xxxxxxxxxxxx

PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc

SEQ RSRRSPLKEGLKYSFPGERPSHSLSRDFKNQTTLLGTTHKNPKAGQVWRPEATR

SEG

PRD ccccccccccceeecccccccccccccccccccccccccccccccccccccccc

Prosite for DKFZphtes3_8gll .2 PS00017 839->847 ATP_GTP_A PDOC00017

(No Pfam data available for DKFZphtes3_8gll .2)

DKFZphtes3_8g5

group: testes derived

DKFZphtes3_8g5 encodes a novel 544 ammo acid protein nearly identical to human KIAA087 protein.

The novel protein is a new splice variant of KIAA087.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes .

KIAA087, alternative spliced complete cDNA, complete eds, EST hits

Sequenced by MediGenomix

Locus : unknown

Insert length: 2762 bp

No poly A stretch found, no polyadenylation signal found

1 CCGACATCGG CCGTGTCTCC AGCACCTGCC GGCGGCTGCG CGAGCTGTGC

51 CAGAGCAGCG GGAAGGTGTG GAAGGAGCAG TTCCGGGTGA GGTGACCTTC

101 CCTTATGAAA CACTACAGCC CCACCGACTA CGTCAATTGG TTGGAAGAGT

151 ATAAAGTTCG GCAAAAAGCT GGGTTAGAAG CGCGGAAGAT TGTAGCCTCG

201 TTCTCAAAGA GGTTCTTTTC AGAGCACGTT CCTTGTAATG GCTTCAGTGA

251 CATTGAGAAC CTTGAAGGAC CAGAGATTTT TTTTGAGGAT GAACTGGTGT

301 GTATCCTAAA TATGGAAGGA AGAAAAGCTT TGACCTGGAA ATACTACGCA

351 AAAAAAATTC TTTACTACCT GCGGCAACAG AAGATCTTAA ATAATCTTAA

401 GGCCTTTCTT CAGCAGCCAG ATGACTATGA GTCGTATCTT GAAGGTGCTG

451 TATATATTGA CCAGTACTGC AATCCTCTCT CCGACATCAG CCTCAAAGAC

501 ATCCAGGCCC AAATTGACAG CATCGTGGAG CTTGTTTGCA AAACCCTTCG

551 GGGCATAAAC AGTCGCCACC CCAGCTTGGC CTTCAAGGCA GGTGAATCAT

601 CCATGATAAT GGAAATAGAA CTCCAGAGCC AGGTGCTGGA TGCCATGAAC

651 TATGTCCTTT ACGACCAACT GAAGTTCAAG GGGAATCGAA TGGATTACTA

701 TAATGCCCTC AACTTATATA TGCATCAGGT TTTGATTCGC AGAACAGGAA

751 TCCCAATCAG CATGTCTCTG CTCTATTTGA CAATTGCTCG GCAGTTGGGA

801 GTCCCACTGG AGCCTGTCAA CTTCCCAAGT CACTTCTTAT TAAGGTGGTG

851 CCAAGGCGCA GAAGGGGCGA CCCTGGACAT CTTTGACTAC ATCTACATAG

901 ATGCTTTTGG GAAAGGCAAG CAGCTGACAG TGAAAGAATG CGAGTACTTG

951 ATCGGCCAGC ACGTGACTGC AGCACTGTAT GGGGTGGTCA ATGTCAAGAA

1001 GGTGTTACAG AGAATGGTGG GAAACCTGTT AAGCCTGGGG AAGCGGGAAG

1051 GCATCGACCA GTCATACCAG CTCCTGAGAG ACTCGCTGGA TCTCTATCTG

1101 GCAATGTACC CGGACCAGGT GCAGCTTCTC CTCCTCCAAG CCAGGCTTTA

1151 CTTCCACCTG GGAATCTGGC CAGAGAAGTC TTTCTGTCTT GTTTTGAAGG

1201 TGCTTGACAT CCTCCAGCAC ATCCAAACCC TAGACCCGGG GCAGCACGGG

1251 GCGGTGGGCT ACCTGGTGCA GCACACTCTA GAGCACATTG AGCGCAAAAA

1301 GGAGGAGGTG GGCGTAGAGG TGAAGCTGCG CTCCGATGAG AAGCACAGAG

1351 ATGTCTGCTA CTCCATCGGG CTCATTATGA AGCATAAGAG GTATGGCTAT

1401 AACTGTGTGA TCTACGGCTG GGACCCCACC TGCATGATGG GACACGAGTG

1451 GATCCGGAAC ATGAACGTCC ACAGCCTGCC GCACGGCCAC CACCAGCCTT

1501 TCTATAACGT GCTGGTGGAG GACGGCTCCT GTCGATACGC AGCCCAAGAA

1551 AACTTGGAAT ATAACGTGGA GCCTCAAGAA ATCTCACACC CTGACGTGGG

1601 ACGCTATTTC TCAGAGTTTA CTGGCACTCA CTACATCCCA AACGCAGAGC

1651 TGGAGATCCG GTATCCAGAA GATCTGGAGT TTGTCTATGA AACGGTGCAG

1701 AATATTTACA GTGCAAAGAA AGAGAACATA GATGAGTAAA GTCTAGAGAG

1751 GACATTGCAC CTTTGCTGCT GCTGCTATCT TCCAAGAGAA CGGGACTCCG

1801 GAAGAAGACG TCTCCACGGA GCCCTCGGGA CCTGCTGCAC CAGGAAAGCC

1851 ACTCCACCAG TAGTGCTGGT TGCCTCCTAC TAAGTTTAAA TACCGTGTGC

1901 TCTTCCCCAG CTGCAAAGAC AATGTTGCTC TCCGCCTACA CTAGTGAATT

1951 AATCTGAAAG GCACTGTGTC AGTGGCATGG CTTGTATGCT TGTCCTGTGG

2001 TGACAGTTTG TGACATTCTG TCTTCATGAG GTCTCACAGT CGACGCTCCT

2051 GTAATCATTC TTTGTATTCA CTCCATTCCC CTGTCTGTCT GCATTTGTCT

2101 CAGAACATTT CCTTGGCTGG ACAGATGGGG TTATGCATTT GCAATAATTT

2151 CCTTCTGATT TCTCTGTGGA ACGTGTTCGG TCCCGAGTGA GGACTGTGTG

2201 TCTTTTTACC CTGAAGTTAG TTGCATATTC AGAGGTAAAG TTGTGTGCTA

2251 TCTTGGCAGC ATCTTAGAGA TGGAGACATT AACAAGCTAA TGGTAATTAG

2301 AATCATTTGA ATTTATTTTT TTCTAATATG TGAAACACAG ATTTCAAGTG

2351 TTTTATCTTT TTTTTTTTTA AATTTAAATG GGAATATAAC ACAGTTTTCC

2401 CTTCCATATT CCTCTCTTGA GTTTATGCAC ATCTCTATAA ATCATTAGTT

2451 TTCTATTTTA TTACATAAAA TTCTTTTAGA AAATGCAAAT AGTGAACTTT

2501 GTGAATGGAT TTTTCCATAC TCATCTACAA TTCCTCCATT TTAAATGACT

2551 ACTTTTATTT TTTAATTTAA AAAATCTACT TCAGTATCAT GAGTAGGTCT

2601 TACATCAGTG ATGGGTTCTT TTTGTAGTGA GACATACAAA TCTGATGTTA 2651 ATGTTTGCTC TTAGAAGTCA TACTCCATGG TCTTCAAAGA CCAAAAAATG 2701 AGGTTTTGCT TTTGTAATCA GGAAAAAAAA AATTAATGAA CCTTAAAAAA 2751 AAAAAAAAAA GG

BLAST Results

No BLAST result

Medline entries

No Medline entry

Peptide information for frame 3

ORF from 105 bp to 1736 bp; peptide length: 544 Category: known protein Classification: unclassified

1 MKHYSPTDYV NWLEEYKVRQ KAGLEARKIV ASFSKRFFSE HVPCNGFSDI 51 ENLEGPEIFF EDELVCILNM EGRKALTWKY YAKKILYYLR QQKILNNLKA 101 FLQQPDDYES YLEGAVYIDQ YCNPLSDISL KDIQAQIDSI VELVCKTLRG 151 INSRHPSLAF KAGESSMIME IELQSQVLDA MNYVLYDQLK FKGNRMDYYN 201 ALNLYMHQVL IRRTGIPISM SLLYLTIARQ LGVPLEPVNF PSHFLLRWCQ 251 GAEGATLDIF DYIYIDAFGK GKQLTVKECE YLIGQHVTAA LYGVVNVKKV 301 LQRMVGNLLS LGKREGIDQS YQLLRDSLDL YLAMYPDQVQ LLLLQARLYF 351 HLGIWPEKSF CLVLKVLDIL QHIQTLDPGQ HGAVGYLVQH TLEHIERKKE 401 EVGVEVKLRS DEKHRDVCYS IGLIMKHKRY GYNCVIYGWD PTCMMGHEWI 451 RNMNVHSLPH GHHQPFYNVL VEDGSCRYAA QENLEYNVEP QEISHPDVGR 501 YFSEFTGTHY IPNAELEIRY PEDLEFVYET VQNIYSAKKE NIDE

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_8g5, frame 3

TREMBLNEW:AB020682_1 gene: "KIAA0875"; product: "KIAA0875 protein"; Homo sapiens mRNA for KIAA0875 protein, partial eds., N = 1, Score = 2832, P = 5.5e-295

>TREMBLNEW:AB020682_1 gene: "KIAA0875"; product: "KIAA0875 protein"; Homo sapiens mRNA for KIAA0875 protein, partial eds. Length = 621

HSPs:

Score = 2832 (424.9 bits). Expect = 5.5e-295, P = 5.5e-295 Identities = 537/544 (98%), Positives = 537/544 (98%)

Query: 1 MKHYSPTDYVNWLEEYKVRQKAGLEARKIVASFSKRFFSEHVPCNGFSDIENLEGPEIFF 60

MKHYSPTDYVNWLEEYKVRQKAGLEARKIVASFSKRFFSEHVPCNGFSDIENLEGPEIFF Sbjct: 85 MKHYSPTDYVNWLEEYKVRQKAGLEARKIVASFSKRFFΞEHVPCNGFSDIENLEGPEIFF 144

Query: 61 EDELVCILNMEGRKALTWKYYAKKILYYLRQQKILNNLKAFLQQPDDYESYLEGAVYIDQ 120

EDELVCILNMEGRKALTWKYYAKKILYYLRQQKILNNLKAFLQQPDDYESYLEGAVYIDQ Sbjct: 145 EDELVCILNMEGRKALTWKYYAKKILYYLRQQKILNNLKAFLQQPDDYESYLEGAVYIDQ 204

Query: 121 YCNPLSDISLKDIQAQIDSIVELVCKTLRGINSRHPSLAFKAGESSMIMEIELQSQVLDA 180

YCNPLSDISLKDIQAQIDSIVELVCKTLRGINSRHPSLAFKAGESSMIMEIELQSQVLDA Sbjct: 205 YCNPLSDISLKDIQAQIDSIVELVCKTLRGINSRHPΞLAFKAGESSMIMEIELQSQVLDA 264

Query: 181 MNYVLYDQLKFKGNRMDYYNALNLYMHQVLIRRTGIPISMSLLYLTIARQLGVPLEPVNF 240

MNYVLYDQLKFKGNRMDYYNALNLYMHQVLIRRTGIPISMSLLYLTIARQLGVPLEPVNF Sbjct: 265 MNYVLYDQLKFKGNRMDYYNALNLYMHQVLIRRTGIPISMSLLYLTIARQLGVPLEPVNF 324

Query: 241 PSHFLLRWCQGAEGATLDIFDYIYIDAFGKGKQLTVKECEYLIGQHVTAALYGVVNVKKV 300

PSHFLLRWCQGAEGATLDIFDYIYIDAFGKGKQLTVKECEYLIGQHVTAALYGVVNVKKV Sb_jct: 325 PSHFLLRWCQGAEGATLDIFDYIYIDAFGKGKQLTVKECEYLIGQHVTAALYGVVNVKKV 384

Query: 301 LQRMVGNLLSLGKREGIDQSYQLLRDSLDLYLAMYPDQVQLLLLQARLYFHLGIWPEKSF 360 LQRMVGNLLSLGKREGIDQSYQLLRDSLDLYLAMYPDQVQLLLLQARLYFHLGIWPEK Sbjct: 385 LQRMVGNLLSLGKREGIDQSYQLLRDSLDLYLAMYPDQVQLLLLQARLYFHLGIWPEK— 442

Query: 361 CLVLKVLDILQHIQTLDPGQHGAVGYLVQHTLEHIERKKEEVGVEVKLRSDEKHRDVCYS 420

VLDILQHIQTLDPGQHGAVGYLVQHTLEHIERKKEEVGVEVKLRSDEKHRDVCYS Sbjct: 443 VLDILQHIQTLDPGQHGAVGYLVQHTLEHIERKKEEVGVEVKLRSDEKHRDVCYS 497

Query: 421 IGLIMKHKRYGYNCVIYGWDPTCMMGHEWIRNMNVHSLPHGHHQPFYNVLVEDGSCRYAA 480

IGLIMKHKRYGYNCVIYGWDPTCMMGHEWIRNMNVHSLPHGHHQPFYNVLVEDGSCRYAA Sbjct: 498 IGLIMKHKRYGYNCVIYGWDPTCMMGHEWIRNMNVHSLPHGHHQPFYNVLVEDGSCRYAA 557

Query: 481 QENLEYNVEPQEISHPDVGRYFSEFTGTHYIPNAELEIRYPEDLEFVYETVQNIYSAKKE 540

QENLEYNVEPQEISHPDVGRYFSEFTGTHYIPNAELEIRYPEDLEFVYETVQNIYSAKKE Sbjct: 558 QENLEYNVEPQEISHPDVGRYFΞEFTGTHYIPNAELEIRYPEDLEFVYETVQNIYSAKKE 617

Query: 541 NIDE 544

NIDE Sbjct: 618 NIDE 621

Pedant information for DKFZphtes3_8g5, frame 3

Report for DKFZphtes3_8g5.3

[LENGTH] 544

[MW] 63307.22

[pi] 5.82

[HOMOL] TREMBL:AB020682_1 gene: "KIAA0875"; product: "KIAA0875 protein"; Homo sapiens mRNA for KIAA0875 protein, partial eds. 0.0

[KW] Alpha_Beta

[KW] LOW_COMPLEXITY 1.84 %

SEQ MKHYSPTDYVNWLEEYKVRQKAGLEARKIVASFSKRFFSEHVPCNGFSDIENLEGPEIFF SEG

PRD cccccccccchhhhhhhhhhhhhchhhhhhhhhhhhhhhcccccccccccccccccceee

SEQ EDELVCILNMEGRKALTWKYYAKKILYYLRQQKILNNLKAFLQQPDDYESYLEGAVYIDQ SEG

PRD eeeeeeeeeeccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccceeecceeeeeee

SEQ YCNPLSDISLKDIQAQIDSIVELVCKTLRGINSRHPSLAFKAGESSMIMEIELQSQVLDA SEG

PRD ccccccccchhhhhhhhhhhhhhhhhhcccccccccceeeecccchhhhhhhhhhhhhhh

SEQ MNYVLYDQLKFKGNRMDYYNALNLYMHQVLIRRTGIPISMSLLYLTIARQLGVPLEPVNF SEG

PRD hhhhhccccccccccchhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhcccccccccc

SEQ PSHFLLRWCQGAEGATLDIFDYIYIDAFGKGKQLTVKECEYLIGQHVTAALYGVVNVKKV SEG

PRD cceeeeeeccccccceeeeeeeeeeeccccceeeeeehhhhhhhhhhhhhhhhhhhhhhh

SEQ LQRMVGNLLSLGKREGIDQSYQLLRDSLDLYLAMYPDQVQLLLLQARLYFHLGIWPEKSF SEG

PRD hhhhhccchhhhhhhhccccccchhhhhhhhhhhccchhhhhhhhhhhhhhcccccceee

SEQ CLVLKVLDILQHIQTLDPGQHGAVGYLVQHTLEHIERKKEEVGVEVKLRSDEKHRDVCYS SEG xxxxxxxxxx

PRD ehhhhhhhhhhhhhccccccccchhhhhhhhhhhhhhhhhhhheeeeecccccceeeecc

SEQ IGLIMKHKRYGYNCVIYGWDPTCMMGHEWIRNMNVHSLPHGHHQPFYNVLVEDGSCRYAA SEG

PRD cccchhhhhhhceeeeecccccccchhhhhhhhhhhccccccccccceeeeecccceeee

SEQ QENLEYNVEPQEISHPDVGRYFSEFTGTHYIPNAELEIRYPEDLEFVYETVQNIYSAKKE SEG

PRD hhhhhhhhcccccccccceeeeccccccccccchhhhhhccchhhhhhhhhhhhhccccc

SEQ NIDE SEG .... PRD CCCC

(No Prosite data available for DKFZphtes3_8g5.3) (No Pfam data available for DKFZphtes3_8g5.3) DKFZphtes3_8mlO

group: nucleic acid management

DKFZphtes3_8mlO encodes a novel 221 amino acid protein with strong similarity to polyadenylate-binding proteins.

The poly (A) -binding protein (PABP) binds to the messenger (mRNA) 3 '-poly (A) tail found on most eukaryotic raRNAs and together with the poly (A) tail has been implicated in governing the stability and the translation of mRNA.

The new protein can find application in modulation of mRNA translation and processing/stability. strong similarity to polyadenylate-binding protein frame shift at Bp 707-710

Sequenced by MediGenomix

Locus : unknown

Insert length: 2107 bp

Poly A stretch at pos. 2052, polyadenylation signal at pos. 2033

1 CGGAAAGGTC GCGGCTTGTG TGCCTGCGGG CAGCCGTGCC GAGAATGAAC 51 CCCAGCACCC CCAGCTACCC AACGGCCTCG CTCTACGTGG GGGACCTCCA

101 CCCCGACGTG ACTGAGGCGA TGCTCTACGA GAAGTTCAGC CCGGCAGGGC

151 CCATCCTCTC CATCCGGATC TGCAGGGACT TGATCACCAG CGGCTCCTCC

201 AACTACGCGT ATGTGAACTT CCAGCATACG AAGGACGCGG AGCATGCTCT

251 GGACACCATG AATTTTGATG TTATAAAGGG CAAGCCAGTA CGCATCATGT

301 GGTCTCAGCG TGATCCATCA CTTCGAAAAA GTGGAGTGGG CAACATATTC

351 GTTAAAAATC TGGATAAGTC CATTAATAAT AAAGCACTGT ATGATACAGT

401 TTCTGCTTTT GGTAACATCC TTTCGTGTAA CGTGGTTTGT GATGAAAATG

451 GTTCCAAGGG TTATGGATTT GTACACTTTG AGACACACGA AGCAGCTGAA

501 AGAGCTATTA AAAAAATGAA CGGAATGCTC CTAAATGGTC GCAAAGTATT

551 TGTTGGACAA TTTAAGTCTC GTAAAGAACG AGAAGCTGAA CTTGGAGCTA

601 GGGCAAAAGA GTTCCCCAAT GTTTACATCA AGAATTTTGG AGAAGACATG

651 GATGATGAGC GCCTTAAGGA TCTCTTTGGC AAGTTCGGGC CCGCCTTAAG

701 TGTGAATTAA TGACCGATGA AAGTGGAAAA TCCAAAGGAT TTGGATTTGT

751 AAGCTTTGAA AGGCATGAAG ATGCACAGAA AGCTGTAGAT GAGATGAATG

801 GAAAGGAGCT CAATGGAAAA CAAATTTACG TTGGTCGAGC TCAGAAAAAA

851 GTGGAACGGC AGACGGAACT TAAGCGCACA TTTGAACAGA TGAAGCAAGA

901 TAGGATCACC AGATACCAGG TTGTTAATCT TTATGTGAAA AATCTTGATG

951 ATGGTATTGA TGATGAACGT CTCCGGAAAG CGTTTTCTCC ATTTGGTACA 1001 ATCACTAGTG CAAAGGTTAT GATGGAAGGT GGTCGCAGCA AAGGGTTTGG 1051 TTTTGTATGT TTCTCCTCCC CAGAAGAAGC CACTAAAGCA GTTACAGAAA 1101 TGAACGGTAG AATTGTGGCC ACAAAGCCAT TGTATGTAGC TTTAGCTCAG 1151 CGCAAAGAAG AGCGCCAGGC TTACCTCACT AACGAGTATA TGCAGAGAAT 1201 GGCAAGTGTA CGAGCTGTGC CCAACCAGCG AGCACCTCCT TCAGGTTACT 1251 TCATGACAGC TGTCCCACAG ACTCAGAACC ATGCTGCATA CTATCCTCCT 1301 AGCCAAATTG CTCGACTAAG ACCAAGTCCT CGCTGGACTG CTCAGGGTGC 1351 CAGACCTCAT CCATTCCAAA ATAAGCCCAG TGCTATCCGC CCAGGTGCTC 1401 CTAGAGTACC ATTTAGTACT ATGAGACCAG CTTCTTCACA GGTTCCACGA 1451 GTCATGTCAA CGCAGCGTGT TGCTAACACA TCAACACAGA CAGTGGGTCC 1501 ACGTCCTGCA GCTGCTGCTG CTGCTGCAGC TACCCCTGCT GTGCGCACGG 1551 TTCCACGGTA TAAATATGCT GCGGGAGTTC GCAATCCTCA GCAACATCGT 1601 AATGCACAGC CACAAGTTAC AATGCAACAG CTTGCTGTTC ATGTACAAGG 1651 TCAGGAAACT TTGACTGCCT CCAGGTTGGC ATCTGCCCCT CCTCAAAAGC 1701 AAAAGCAAAT GTTAGGTGAA CGGCTCTTTC CTCTTATTCA AGCCATGCAC 1751 CCTACTCTTG CTGGGAAAAT CACTGGCATG TTGTTGGAGA TTGATAATTC 1801 AGAACTTCTT TATATGCTCG AGTCTCCAGA GTCACTCCGT TCTAAGGTTG 1851 ATGAAGCTGT AGCTGTACTA CAAGCCCACC AAGCTAAAGA GGCTACCCAG 1901 AAAGCAGTTA ACAGTGCTAC CGGTGTTCCA ACTGTTTAAA ATTGATCAGA 1951 GACCACGAAA AGAAATTTGT GCTTCACCGA AGAAAAATAT CTAAACATCG 2001 AGAAACTATG GGAAAAAAAA TTGCAAAATC TAAAATAAAA AATGCAAAAT 2051 CTAAAATAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 2101 AAAAAGG

BLAST Results

Entry HSPOLYAB from database EMBL:

Human mRNA for polyA binding protein

Score = 5420, P = O.Oe+00, identities = 1162/1243 Medlme entries

No Medline entry

Peptide information for frame 2

ORF from 707 bp to 1936 bp; peptide length: 410 Category: strong similarity to known protein Classification: unset Prosite motifs: RNP_1 (10-18) RNP 1 (112-120)

1 LMTDESGKSK GFGFVSFERH EDAQKAVDEM NGKELNGKQI YVGRAQKKVE

51 RQTELKRTFE QMKQDRITRY QVVNLYVKNL DDGIDDERLR KAFSPFGTIT

101 SAKVMMEGGR SKGFGFVCFS SPEEATKAVT EMNGRIVATK PLYVALAQRK

151 EERQAYLTNE YMQRMASVRA VPNQRAPPSG YFMTAVPQTQ NHAAYYPPSQ

201 IARLRPSPRW TAQGARPHPF QNKPSAIRPG APRVPFSTMR PASSQVPRVM

251 STQRVANTST QTVGPRPAAA AAAAATPAVR TVPRYKYAAG VRNPQQHRNA

301 QPQVTMQQLA VHVQGQETLT ASRLASAPPQ KQKQMLGERL FPLIQAMHPT

351 LAGKITGMLL EIDNSELLYM LESPESLRSK VDEAVAVLQA HQAKEATQKA

401 VNSATGVPTV

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_8mlO, frame 2

PIR:DNHUPA polyadenylate-bindmg protein - human, N = 1, Score = 1931, P = 1.7e-199

PIR:I48718 poly(A) binding protein - mouse, N = 1, Score = 1928, P = 3.6e-199

>PIR:DNHUPA polyadenylate-binding protein - human Length = 633

HSPs:

Score = 1931 (289.7 bits), Expect = 1.7e-199, P = 1.7e-199 Identities = 384/415 (92%), Positives = 394/415 (94%)

Query: 1 LMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRTFE 60

+MTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKR FE Sbjct: 219 VMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFE 278

Query: 61 QMKQDRITRYQVVNLYVKNLDDGIDDERLRKAFSPFGTITSAKVMMEGGRSKGFGFVCFS 120

QMKQDRITRYQ VNLYVKNLDDGIDDERLRK FSPFGTITSAKVMMEGGRSKGFGFVCFS Sbjct: 279 QMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFS 338

Query: 121 SPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAYLTNEYMQRMASVRAVPN Q 174

SPEEATKAVTEMNGRIVATKPLYVALAQRKEERQA+LTN+YMQRMASVRAVPN Q Sbjct: 339 SPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQ 398

Query: 175 RAPPSGYFMTAVPQTQNHAAYYPPSQIARLRPSPRWTAQGARPHPFQNKPSAIRPGAPRV 234

APPSGYFM A+PQTQN AAYYPPSQ+A+LRPSPRWTAQGARPHPFQN P AIRP APR Sbjct: 399 PAPPSGYFMAAIPQTQNRAAYYPPSQVAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRP 458

Query: 235 PFSTMRPASSQVPRVMSTQRVANTSTQTVGPRPAAAAAAAATPAVRTVPRYKYAAGVRNP 294

PFSTMRPASSQVPRVMSTQRVANTSTQT+GPRPAAAAAAA TPAVRTVP+YKYAAGVRNP Sbjct: 459 PFSTMRPASSQVPRVMSTQRVANTΞTQTMGPRPAAAAAAA-TPAVRTVPQYKYAAGVRNP 517

Query: 295 QQHRNAQPQVTMQQLAVHVQGQETLTAΞRLASAPPQKQKQMLGERLFPLIQAMHPTLAGK 354

QQH NAQPQVTMQQ AVHVQGQE LTAS LASAPPQ+QKQMLGERLFPLIQAMHPTLAGK Sbjct: 518 QQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGK 577

Query: 355 ITGMLLEIDNSELLYMLESPESLRSKVDEAVAVLQAHQAKEATQKAVNSATGVPTV 410

ITGMLLEIDNSELL+MLESPESLRSKVDEAVAVLQAHQAKEA QKAVNSATGVPTV Sb_jct: 578 ITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV 633

Score = 315 (47.3 bits), Expect = 1.9e-27, P = 1.9e-27 Identities = 71/163 (43%), Positives = 102/163 (62%)

Query: 1 LMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRTFE 60

++ DE+G SKG+GFV FE E A++A+++MNG LN ++++VGR + + ER+ EL + Sbjct: 130 VVCDENG-SKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAK 188

Query: 61 QMKQDRITRYQVVNLYVKNLDDGIDDERLRKAFSPFGTITSAKVMM-EGGRSKGFGFVCF 119

+ N+Y+KN + +DDERL+ F P S KVM E G+ΞKGFGFV F Sbjct: 189 EF TNVYIKNFGEDMDDERLKDLFGP ALΞVKVMTDESGKSKGFGFVSF 235

Query: 120 SSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAYLTNEYMQ 163

E+A KAV EMNG+ + K +YV AQ+K ERQ L ++ Q Sbjct: 236 ERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQ 279

Score = 214 (32.1 bits), Expect = 1.9e-14, P = 1.9e-14 Identities = 50/150 (33%), Positives = 87/150 (58%)

Query: 8 KSKGFGFVΞFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRTFEQMKQDRI 67

+S G+ +V+F++ DA++A+D MN + GK + + +Q R L+++ Sbjct: 50 RSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQ RDPSLRKS 96

Query: 68 TRYQVVNLYVKNLDDGIDDERLRKAFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATK 127

V N+++KNLD ID++ L FS FG I S KV+ + SKG+GFV F + E A + Sbjct: 97 GVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAER 153

Query: 128 AVTEMNGRIVATKPLYVALAQRKEERQAYL 157

A+ +MNG ++ + ++V + ++ER+A L Sbjct: 154 AIEKMNGMLLNDRKVFVGRFKSRKEREAEL 183

Score = 120 (18.0 bits), Expect = 4.8e-04, P = 4.8e-04 Identities = 30/99 (30%), Positives = 54/99 (54%)

Query: 70 YQVVNLYVKNLDDGIDDERLRKAFSPFGTITSAKVM—MEGGRSKGFGFVCFSSPEEATK 127

Y + +LYV +L + + L + FSP G I S +V M RS G+ +V F P +A + Sbjct: 8 YPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAER 67

Query: 128 AVTEMNGRIVATKPLYVALAQRKEE-RQAYLTNEYMQRM 165

A+ MN ++ KP+ + +QR R++ + N +++ + Sbjct: 68 ALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNL 106

Peptide information for frame 3

ORF from 45 bp to 707 bp; peptide length: 221 Category: strong similarity to known protein Classification: unset Prosite motifs: RNP 1 (138-146)

1 MNPSTPSYPT ASLYVGDLHP DVTEAMLYEK FSPAGPILSI RICRDLITSG

51 SSNYAYVNFQ HTKDAEHALD TMNFDVIKGK PVRIMWSQRD PSLRKSGVGN

101 IFVKNLDKSI NNKALYDTVS AFGNILSCNV VCDENGSKGY GFVHFETHEA

151 AERAIKKMNG MLLNGRKVFV GQFKSRKERE AELGARAKEF PNVYIKNFGE

201 DMDDERLKDL FGKFGPALSV N

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_8mlO, frame 3

SWISSPROT :PAB1_HUMAN POLYADENYLATE-BINDING PROTEIN 1 (POLY (A) BINDING PROTEIN 1) (PABP 1)., N = 1, Score = 1039, P = 5.7e-105

PIR:I48718 poly(A) binding protein - mouse, N = 1, Score = 1031, P = 4e-104

PIR:DNHUPA polyadenylate-binding protein - human, N = 1, Score = 1009, P = 8.7e-102

>SWISSPROT:PABl_HUMAN POLYADENYLATE-BINDING PROTEIN 1 (POLY (A) BINDING PROTEIN 1) (PABP 1) . Length = 636

HSPs: Score = 1039 (155.9 bits), Expect = 5.7e-105, P = 5.7e-105 Identities = 199/220 (90%), Positives = 205/220 (93%)

Query: 1 MNPSTPSYPTASLYVGDLHPDVTEAMLYEKFSPAGPILSIRICRDLITSGSSNYAYVNFQ 60

MNPS PSYP ASLYVGDLHPDVTEAMLYEKFSPAGPILSIR+CRD+IT S YAYVNFQ Sb_jct: 1 MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQ 60

Query: 61 HTKDAEHALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFVKNLDKSINNKALYDTVS 120

DAE ALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIF+KNLDKSI+NKALYDT S Sbjct: 61 QPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFS 120

Query: 121 AFGNILSCNVVCDENGSKGYGFVHFETHEAAERAIKKMNGMLLNGRKVFVGQFKSRKERE 180

AFGNILSC VVCDENGSKGYGFVHFET EAAERAI+KMNGMLLN RKVFVG+FKSRKERE Sbjct: 121 AFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKERE 180

Query: 181 AELGARAKEFPNVYIKNFGEDMDDERLKDLFGKFGPALSV 220

AELGARAKEF NVYIKNFGEDMDDERLKDLFGKFGPALSV Sbjct: 181 AELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSV 220

Score = 275 (41.3 bits), Expect = 4. le-23, P = 4. le-23 Identities = 71/233 (30%), Positives = 120/233 (51%)

Query: 2 NPSTPSYPTASLYVGDLHPDVTEAMLYEKFSPAGPILSIRICRDLITSGSSNYAYVNFQH 61

+PS ++++ +L + LY+ FS G ILS ++ D S + + Q Sbjct: 90 DPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILΞCKVVCDENGSKGYGFVHFETQE 149

Query: 62 TKD-AEHALDTMNFDVIKGKPVRIMW-SQRDPSL--RKSGVGNIFVKNLDKSINNKALYD 117

+ A ++ M + K R +R+ L R N+++KN + ++++ L D Sbjct: 150 AAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKD 209

Query: 118 TVSAFGNILSCNVVCDENG-SKGYGFVHFETHEAAERAIKKMNGMLLNGRKVFVGQFKSR 176

FG LS V+ DE+G SKG+GFV FE HE A++A+ +MNG LNG++++VG+ + + Sbjct: 210 LFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKK 269

Query: 177 KEREAELGARAKEFP NVYIKNFGEDMDDERLKDLFGKFGPALS 219

ER+ EL + ++ N+Y+KN + +DDERL+ F FG S

Sbjct: 270 VERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITS 322

Score = 227 (34.1 bits), Expect = 6.3e-18, P = 6.3e-18 Identities = 57/187 (30%), Positives = 101/187 (54%)

Query: 12 SLYVGDLHPDVTEAMLYEKFSPAGPILSIRICRDLITSGSSNYAYVNFQHTKDAEHALDT 71

++Y+ + D+ + L + F GP LS+++ D + S + +V+F+ +DA+ A+D Sbjct: 192 NVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDE-SGKSKGFGFVSFERHEDAQKAVDE 250

Query: 72 MNFDVIKGKPVRIMWSQR DPSLRKSGVGNIFVKNLDKSINNKA 114

MN + GK + + +Q+ D R GV N++VKNLD I+++

Sbjct: 251 MNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGV-NLYVKNLDDGIDDER 309

Query: 115 LYDTVSAFGNILSCNVVCDENGSKGYGFVHFETHEAAERAIKKMNGMLLNGRKVFVGQFK 174

L S FG I S V+ + SKG+GFV F + E A +A+ +MNG ++ + ++V + Sbjct: 310 LRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQ 369

Query: 175 SRKEREAEL 183

++ER+A L Sbjct: 370 RKEERQAHL 378

Score = 100 (15.0 bits), Expect = 2.3e-02, P = 2.3e-02 Identities = 26/99 (26%), Positives = 53/99 (53%)

Query: 8 YPTASLYVGDLHPDVTEAMLYEKFSPAGPILSIRICRDLITSG-SSNYAYVNFQHTKDAE 66

Y +LYV +L + + L ++FSP G I S ++ ++ G S + +V F ++A Sbjct: 291 YQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKV MMEGGRSKGFGFVCFSSPEEAT 347

Query: 67 HALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFVKNL 106

A+ MN ++ KP+ + +QR R++ + N +++ + Sbjct: 348 KAVTEMNGRIVATKPLYVALAQRKEE-RQAHLTNQYMQRM 386

Pedant information for DKFZphtes3_8mlO, frame 2

Report for DKFZphtes3_8mlO .2

[LENGTH] 409

[MW] 45235.68

[pi] 10.08

[HOMOL] SWISSPROT :PAB1_HUMAN POLYADENYLATE-BINDING PROTEIN 1 (POLY (A) BINDING PROTEIN

1) (PABP 1) . 0.0 [FUNCAT] 04.05.05 mrna processing (5 '-end, 3 '-end processing and mrna degradation) [S. cerevisiae, YER165w] le-54

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YER165w] le-54

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YER165w] le-54

[FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae,

YER165w] le -54

[FUNCAT] 04.05.99 other mrna-transcπption activities [S. cerevisiae, YNL016w] le-15

[FUNCAT] 11.01 stress response [S. cerevisiae, YGR159c] le-12

[ FUNCAT ] 04.01.04 rrna processing [S. cerevisiae, YGR159c) le-12

[ FUNCAT ] 04.99 other transcription activities [S. cerevisiae, YNL175C] 4e-09

[ FUNCAT ] 98 classification not yet clear-cut [S. cerevisiae, YPR112c] 5e-08

[FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YHR086w] 3e-07

[FUNCAT] 03.13 meiosis [S. cerevisiae, YHR086w] 3e-07

[FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YHR086w] 3e-07

[FUNCAT] 04.07 rna transport [S. cerevisiae, YOL12₃w HRPl - CF Ib] 9e-07

[FUNCAT] 30.13 organization of chromosome structure [S. cerevisiae, YCLOllc] 3e-06

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YGR250c] 8e-06

[FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YDR432w]

2e-05

[FUNCAT] 08.01 nuclear transport [S. cerevisiae, YDR432w] 2e-05

[FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [Ξ. cerevisiae, YFR023w] 3e-05

[FUNCAT] 03.01 cell growth [S. cerevisiae, YBR212w] 3e-04

[BLOCKS] BL00030B Eukaryotic RNA-binding region RNP-1 proteins

[SCOP] dlsxl 4.34.7.1.3 Sex-lethal protein [(Drosophila melanogaster) le-17

[PIRKW] nucleus 0.0

[PIRKW] duplication 0.0

[PIRKW] RNA binding 0.0

[PIRKW] nucleolus 2e-09

[PIRKW] tandem repeat 2e-09

[PIRKW] single-stranded DNA binding 3e-06

[PIRKW] DNA binding 5e-13

[PIRKW] phosphoprotem 6e-10

[PIRKW] ribosome 3e-08

[PIRKW] mitochondrion 3e-08

[PIRKW] alternative splicing 9e-ll

[PIRKW] chloroplast 2e-19

[PIRKW] transcription regulation 2e-07

[PIRKW] protein biosynthesis 3e-08

[SUPFAM] nucleolm 6e-10

[SUPFAM] glycine-rich RNA-binding protein 2e-07

[SUPFAM] unassigned ribonucleoprotein repeat-containing proteins 2e-19

[SUPFAM] polyadenylate-binding protein 0.0

[SUPFAM] ribonucleoprotein repeat homology 0.0

[PROSITE] RNP_1 2

[PFAM] RNA recognition motif, (aka RRM, RBD, or RNP domain)

[KW] Irregular

[KW] 3D

[KW] LOW COMPLEXITY 5.62 %

SEQ MTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRTFEQ SEG lsxl-

SEQ MKQDRITRYQVVNLYVKNLDDGIDDERLRKAFSPFGTITSAKVMMEGGRSKGFGFVCFSS SEG lsxl- CEEEECCCTTTTHHHHHHHHTTTTCCCCCEEECTTTCTTTEEEECTTT

SEQ PEEATKAVTEMNGRIVATKPLYVALAQRKEERQAYLTNEYMQRMASVRAVPNQRAPPSGY SEG lsxl- HHHHHHHHHHHTTTCCCCCCCBCCBCC

SEQ FMTAVPQTQNHAAYYPPSQIARLRPSPRWTAQGARPHPFQNKPSAIRPGAPRVPFSTMRP SEG lsxl-

SEQ ASSQVPRVMSTQRVANTSTQTVGPRPAAAAAAAATPAVRTVPRYKYAAGVRNPQQHRNAQ SEG xxxxxxxxxxxxxxxxxxxxxxx lsxl-

SEQ PQVTMQQLAVHVQGQETLTASRLASAPPQKQKQMLGERLFPLIQAMHPTLAGKITGMLLE SEG lsxl-

SEQ IDNSELLYMLESPESLRSKVDEAVAVLQAHQAKEATQKAVNSATGVPTV SEG lsxl- Prosite for DKFZphtes3_8mlO .2

PS00030 9->17 RNP_ PDOC00030 PS00030 111->119 RNP PDOC00030

Pfam for DKFZphtes3_8ml0.2

HMM_NAME RNA recognition motif, (aka RRM, RBD, or RNP domain)

HMM *IYVGNLPWDtTEEDLrDlFsQFGpIvsIrMMrDReTGRSRGFAFVEFED

+YV+NL+ +++E LR +FS+FG I+S+++M+ E GRS+GF+FV F + Query 74 LYVKNLDDGIDDERLRKAFSPFGTITSAKVM —EGGRSKGFGFVCFSS 120

HMM EEDAekAIdeMNGmeFmGRrlRV*

+E+A+KA+ EMNG+++ ++++V Query 121 PEEATKAVTEMNGRIVATKPLYV 143

Pedant information for DKFZphtes3_8ml0, frame 3

Report for DKFZphtes3_8mlO .3

[LENGTH] 235

[MW] 26308.08

[pi] 8.95

[HOMOL] SWISSPROT :PAB1_HUMAN POLYADENYLATE-BINDING PROTEIN 1 (POLY (A) BINDING PROTEIN

1) (PABP 1) , le-113

[FUNCAT] 04.05.05 mrna processing (5 '-end, 3 ' -end processing and mrna degradation) [S. cerevisiae, YER165w] le-64

[FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YER165w] le-64

[FUNCAT) 05.04 translation (initiation, elongation and termination) [S. cerevisiae.

YER165w] le -64

[FUNCAT] 30.10 nuclear organization [S. cerevisiae, YER165w] le-64

[FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YFR023w] le-24

[FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YFR023w] le-24

[FUNCAT] 04.05.99 other mrna-transcπption activities [S. cerevisiae, YNL016w]

2e-19

[ FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YOR319w] 2e-14

[FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YGR159c] le-11

[FUNCAT] 11.01 stress response [S. cerevisiae, YGR159c] le-11

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YGR250c] le-09

[FUNCAT] 04.07 rna transport [S. cerevisiae, YOL123w HRP1 - CF Ib] le-09

[FUNCAT] 30.13 organization of chromosome structure [S. cerevisiae, YCLOllc] 8e-09

[FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YPR112c] 2e-08

[FUNCAT] 03.13 meiosis [S. cerevisiae, YHR086w] 2e-08

[FUNCAT] 04.99 other transcription activities [S. cerevisiae, YBR212w] 3e-08

[FUNCAT] 03.01 cell growth [S. cerevisiae, YBR212w] 3e-08

[FUNCAT] 06.04 protein targeting, sorting and translocation [Ξ. cerevisiae, YDR432w]

3e-04

[FUNCAT] 08.01 nuclear transport [Ξ. cerevisiae, YDR432w] 3e-04

[BLOCKS] BL00030B Eukaryotic RNA-binding region RNP-1 proteins

[BLOCKS] BL00900D Bacteriophage-type RNA polymerase family proteins signatur

[SCOP] dlsxl 4.34.7.1.3 Sex-lethal protein [ (Drosophila melanogaster) 9e-23

[SCOP] d2ula 4.34.7.1.2 UlA protein [human (Homo sapiens) 6e-24

[SCOP] dlupl_2 4.34.7.1.1 Nuclear ribonucleoprotein Al, RNP Al, UP le-13

[PIRKW] nucleus le-110

[PIRKW] duplication le-110

[PIRKW] RNA binding le-110

[PIRKW] nucleolus 4e-10

[PIRKW] tandem repeat 4e-10

[PIRKW] single-stranded DNA binding le-06

[PIRKW] DNA binding 9e-12

[PIRKW] phosphoprotein 4e-10

[PIRKW] mitochondrion 6e-07

[PIRKW] heterotrimer 4e-06

[PIRKW] alternative splicing le-15

[PIRKW] chloroplast 5e-ll

[PIRKW] transcription regulation 3e-09

[PIRKW] GTP binding 2e-06

[SUPFAM] helix-destabilizing protein le-07

[SUPFAM] nucleolin 4e-10

[SUPFAM] glycine-rich RNA-bind g protein 2e-07

[SUPFAM] yeast HRP1 protein 2e-08 [SUPFAM] unassigned ribonucleoprotein repeat-containmg proteins 3e-25

[SUPFAM] polyadenylate-b ndmg protein le-112

[SUPFAM] ribonucleoprotein repeat homology le-112

[PROSITE] RNP_1 1

[PFAM] RNA recognition motif, (aka RRM, RBD, or RNP domain)

[KW] All_Beta

[KW] 3D

SEQ ERSRLVCLRAAVPRMNPSTPSYPTASLYVGDLHPDVTEAMLYEKFSPAGPILSIRICRDL lhal- EEEETTTTTTCHHHHHHHHGGGCCEEEEEEEETT

SEQ ITSGSSNYAYVNFQHTKDAEHALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFVKNL lhal- TTTCEEEEEEEEECCHHHHHHHHHHTTEEE-TT EEEEEEECTTTTCCCCCEEEEECC

SEQ DKSINNKALYDTVSAFGNILSCNVVCDENGSKGYGFVHFETHEAAERAIKKMNGMLLNGR lhal- TTTTCHHHHHHHHGGGCCEEEEEEEETTTTTCEEEEEEECCHHHHHHHH

SEQ KVFVGQFKSRKEREAELGARAKEFPNVYIKNFGEDMDDERLKDLFGKFGPALSVN lhal-

Prosite for DKFZphtes3_8mlO .3 PS00030 152->160 RNP_1 PDOC00030

Pfam for DKFZphtes3_8mlO .3

HMM_NAME RNA recognition motif, (aka RRM, RBD, or RNP domain)

HMM *IYVGNLPWDtTEEDLrDlFsQFGpIvsIrMMrDReTGRSRGFAFVEFED

+YVG+L +D+TE +L + FS+ GPI+SIR+ RD T S +A+V+F+ Query 27 LYVGDLHPDVTEAMLYEKFSPAGPILSIRICRDLITSGSSNYAYVNFQH 75

HMM EEDAekAIdeMNGmeFmGRrlRV*

DAE A+D+MN ++ G+++R+ Query 76 TKDAEHALDTMNFDVIKGKPVRI 98

HMM *IYVGNLPWDtTEEDLrDlFsQFGpIvsIrMMrDReTGRSRGFAFVEFED

I+V+NL+ +++ L D S FG I+S++++ D + S+G++FV FE+ Query 115 IFVKNLDKSINNKALYDTVSAFGNILSCNVVCD—ENGSKGYGFVHFET 161

HMM EEDAekAIdeMNGmeFmGRrlRV*

+E+AE+AI +MNGM+++GR++ V Query 162 HEAAERAIKKMNGMLLNGRKVFV 184

DKFZphtes3_8p7

group: testes derived

DKFZphtes3_8p7 encodes a novel 412 amino acid protein without similarity to known proteins.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . unknown

2 EST hits (both from testis librarys)

Sequenced by MediGenomix

Locus: unknown

Insert length: 2899 bp

Poly A stretch at pos. 2870, polyadenylation signal at pos. 2852

1 CCGACCCGCC CTGGGGTGCT GCGTGCGCTG CCTGCTCCCG CCTGAGGAAA

51 ACACTGCCCA TGGCGCAAGG CCGGGAGCGC GACGAAGGCC CCCACTCCGC

101 CGGCGGCGCG TCCTTGTCCG TGAGATGGGT GCAAGGATTC CCTAAGCAGA

151 ATGTTCATTT GTCAACGACA ACACCATTTG CTACCCTTGT GGGAATTATG

201 TAATATTTAT TAATATTGAA ACCAAGAAAA AGACTGTACT GCAGTGTAGT

251 AATGGAATTG TGGGCGTCAT GGCAACTAAC ATCCCCTGTG AAGTTGTGGC

301 TTTTTCTGAC CGGAAGCTAA AACCTCTCAT CTACGTATAC AGCTTTCCAG

351 GATTGACCAG AAGGACCAAA TTGAAAGGCA ACATTCTCCT GGACTACACT

401 TTACTTTCAT TCAGTTACTG TGGCACCTAC CTGGCTAGTT ACTCCTCTCT

451 CCCAGAATTT GAACTGGCCC TTTGGAACTG GGAATCGAGT ATCATTTTGT

501 GTAAGAAATC ACAGCCTGGA ATGGATGTGA ACCAAATGTC TTTTAACCCC

551 ATGAACTGGC GCCAGCTGTG CTTATCAAGT CCAAGTACAG TGAGCGTGTG

601 GACCATTGAA AGAAGTAACC AGGAGCATTG TTTCAGAGCA AGGTCGGTGA

651 AATTACCTCT AGAAGATGGG TCATTTTTTA ATGAAACGGA TGTCGTTTTC

701 CCCCAGTCGT TGCCGAAAGA TCTCATCTAT GGTCCCGTGC TGCCACTGTC

751 AGCCATTGCC GGGCTGGTAG GCAAAGAGGC AGAGACTTTC CGGCCGAAAG

801 ATGATCTATA TCCTTTGCTT CACCCGACTA TGCATTGCTG GACTCCAACA

851 AGTGACTTGT ACATTGGCTG TGAAGAGGGT CATCTTTTAA TGATTAATGG

901 AGACACCTTG CAAGTGACTG TACTTAATAA GATAGAAGAG GAATCGCCAT

951 TGGAAGACAG AAGAAATTTT ATCAGTCCAG TAACCTTGGT ATATCAGAAG

1001 GAGGGCGTGC TGGCTTCTGG AATTGATGGC TTTGTGTATT CTTTTATTAT

1051 TAAAGATAGA AGTTACATGA TCGAGGATTT TCTTGAGATT GAAAGACCTG

1101 TAGAACATAT GACATTTTCT CCCAATTATA CAGTGTTGCT GATTCAAACA

1151 GACAAGGGAT CTGTTTATAT CTACACTTTT GGTAAGGAGC CAACCTTAAA

1201 TAAAGTCCTA GATGCTTGTG ATGGGAAATT TCAGGCAATT GACTTTATCA

1251 CACCTGGAAC CCAATACTTC ATGACACTTA CATATTCAGG GGAAATTTGT

1301 GTTTGGTGGC TGGAGGATTG TGCTTGTGTA AGCAAGATTT ATCTGAATAC

1351 CCTAGCAACG GTTCTGGCTT GCTGTCCATC CTCCCTCTCT GCAGCCGTGG

1401 GCACGGAGGA TGGCTCGGTC TACTTCATCA GCGTATATGA TAAGGAATCC

1451 CCTCAGGTCG TGCACAAGGC CTTTCTCTCG GAATCGTCCG TGCAGCACGT

1501 CGTGTAAGTC CTTTCTGCCT CCAGGAGCGG CTCCGTGTCA CACCCGTCTG

1551 TTGAAAATTC TAGTGAAGCC ATCCTTTCTT TTAATTTTAA GTTTTACGTG

1601 TTTCATTTGT TTTGAATGTT AATATATTCA CACAGTTCAA CACTCAAAAG

1651 GTACAGAGGG CTGTGTAGTA AAGTACCCCC CATACCCAGG TCTGTCCTTG

1701 CAGGCAGCCT GGTACCAATT TCTCATGTCT CTCCTGAGAT GTTTTATCCA

1751 TGAACAAGCA AAACATAATA AGCACTTCTT TTTACTTGTA TCAATGGCCA

1801 TCATGTGTGT ATAGTGTGCC AGGCACTTCT GCTGTATTAA CTCCATGAGG

1851 TAAACACTCT TGTTGTCTCT ATTTGACAGG TGAGGAAGAT AAGGCACAAG

1901 GATTTTAAAT AACTTGCTCA ATAGTACACA GATAGTGAAT GGCAAATGTT

1951 GGGATTTGAA CCCAGGTAGT TGGGCTGCAG AGTCACTGCC TTTGCTCTTA

2001 AAAGGAGAAA ACTATGTACA ATGCCTCATT TCTTTTTTCA CTTAATCGTA

2051 TATCTTGGAG AATGTTTTAT ATCCACACAT AAAGACCAGC CTGATTATTT

2101 GTATAGCCAC ATAGTATTCC ATTATATGAA TATACTATCA TTTTTTAAAA

2151 ACGGTATATT AATGAACATT TAGAGTATTT CAAAACTTTT GAAGCAATAC

2201 TTTTAAGATG ATAATATAGA GACATTAGAT TTGGACTTGT AGGTGCTATC

2251 ATTATTACTG TTTCTTTTTA ATTTATTATA TTATTAGGTA TTAATAAGAA

2301 CAGACATTTG TATTCTGCTT TACAGCTTGA GATCACTGTA GCTTGTGGCA

2351 TGTGATCCTC AAAACACCAG TCAGAAAGGT GTTATTCTTA TCCCTATTAG

2401 ACAAATTAGG GAATTCAGGG TTAGAGAGGT GAGGAAAAGC ATTGTCCAAG

2451 ATTACACATT ACACAGCTAG CACACTGAGG AGCTGGCCCT GCCACTGTGG

2501 ACTGCCCAGC TCCACCACCC TAGCTCAGTG GGGAAGGATG GATAACCTCC

2551 TTCCATTTAC CCCCTGCCTT TCTGCACTGT CATTTTTTTG TGCCTTTCCT

2601 TTCTCAGATC CTCTTATTCT AATTTACATC TTCCCACTTT TTCTAATTTG

2651 ATAAAGTTGT AGACATGTTT CACTACATTC TTCCTCCCAC TGCCAGGTAC

2701 CAGACACAGG GTAATGAAAT GTCACACCCA CCACTAATTT GAGAATTGCT 2751 TATTTGCGCT TGAAACATCA AGAAAGCTCT ACCGACAGAC ATGTTTCATT 2801 CACTTATGAT GAACCAACTG CCCATCTTTA CTGAATCTTC TTGACTGTAT 2851 TTATTAAAGT TGCAATTTGG AAATAAAAAA AAAAAAAAAA AAAAAAAGG

BLAST Results

No BLAST result

Medline entries

No Medlme entry

Peptide information for frame 2

ORF from 269 bp to 1504 bp; peptide length: 412 Category: putative protein Classification: no clue

1 MATNIPCEVV AFSDRKLKPL IYVYSFPGLT RRTKLKGNIL LDYTLLSFSY 51 CGTYLASYSS LPEFELALWN WESSIILCKK SQPGMDVNQM SFNPMNWRQL 101 CLSSPSTVSV WTIERSNQEH CFRARSVKLP LEDGSFFNET DVVFPQSLPK 151 DLIYGPVLPL SAIAGLVGKE AETFRPKDDL YPLLHPTMHC WTPTSDLYIG 201 CEEGHLLMIN GDTLQVTVLN KIEEESPLED RRNFISPVTL VYQKEGVLAS 251 GIDGFVYSFI IKDRSYMIED FLEIERPVEH MTFSPNYTVL LIQTDKGSVY 301 IYTFGKEPTL NKVLDACDGK FQAIDFITPG TQYFMTLTYΞ GEICVWWLED 351 CACVSKIYLN TLATVLACCP SSLSAAVGTE DGSVYFISVY DKESPQVVHK 401 AFLSESSVQH VV

BLASTP hits No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_8p7, frame 2 No Alert BLASTP hits found

Pedant information for DKFZphtes3_8p7, frame 2

Report for DKFZphtes3_8p7.2

[LENGTH] 412 [MW] 46476.62 [pi] 4.91 [KW] Alpha_Beta

SEQ MATNIPCEVVAFSDRKLKPLIYVYSFPGLTRRTKLKGNILLDYTLLSFSYCGTYLASYSS PRD cccccceeeeeecccccceeeeeecccccccccccchhhhhhhheeeecccccccccccc

SEQ LPEFELALWNWESΞIILCKKSQPGMDVNQMSFNPMNWRQLCLSSPSTVSVWTIERSNQEH PRD cchhhhhhhhccccceeeccccccccceeeccccccceeeeeccccceeeeeeeecchhh

SEQ CFRARSVKLPLEDGSFFNETDVVFPQSLPKDLIYGPVLPLSAIAGLVGKEAETFRPKDDL PRD hhhhhhhcccccccccccccccccccccccccccccccceeeeeeccccccccccccccc

SEQ YPLLHPTMHCWTPTSDLYIGCEEGHLLMINGDTLQVTVLNKIEEESPLEDRRNFISPVTL PRD cccccccccccccccceeeecccceeeecccceeeeeehhhhhcccccccccccccccee

SEQ VYQKEGVLASGIDGFVYSFIIKDRSYMIEDFLEIERPVEHMTFSPNYTVLLIQTDKGSVY PRD eeeceeeeecccceeeeeeeeeccchhhhhhhhhhcccceeeccccceeeeeecccccee

SEQ IYTFGKEPTLNKVLDACDGKFQAIDFITPGTQYFMTLTYSGEICVWWLEDCACVSKIYLN PRD eeeccccccchhhhhcccccceeeeeccccceeeeeeeccceeeeeeecceeeeeeeehh

SEQ TLATVLACCPSΞLSAAVGTEDGSVYFIΞVYDKESPQVVHKAFLSESSVQHVV PRD hhhhhhhccccccceeeeccccceeeeeeeccccccchhhhhhhcccccccc

(No Prosite data available for DKFZphtes3_8p7.2) (No Pfam data available for DKFZphtes3_8p7.2) DKFZphtes3_9e22

group: testes derived

DKFZphtes3_9e22 encodes a novel 227 amino acid protein with weak partial similarity to Ring- finger proteins.

For the novel protein, Pfam, but not Prosite predicts a C3HC4 type RING fmger motife. No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application m studying the expression profile of testis-specific genes . similarity to zmc finger proteins

Sequenced by DKFZ

Locus : unknown

Insert length: 1318 bp

Poly A stretch at pos. 1308, no polyadenylation signal found

1 GCTCCCCCGG CTTTCGGAGC CCGGGGGCGG CCTGTGGCGC GCGGAGCCCG

51 CGCCGGACTG CGCCTCTTTG GACCTTGAGG GGAAACATGC GTTTGCCTTG

101 GATCGTTTGA AATTCTAAGT TTGGGATCCC CGCCCGCCCG CCTGCCTCTT

151 CCGCCCCGCG GGTTTTTTCC TTTTTTCCTT TTGCTTTTTT TCCTTTTCTC

201 CCTCCGGGTC TCCTTTTTGA CTCCCTCCCC CTTTATGCTC GCCCAGCCCT

251 CCCCCTGCTG CTGAGAAGTG GGGGAGGGTC TCGGCCTCCA GGTTCCCGCC

301 CCACCGGGGC CCGGGCGAGC ATGGGGGGCA AGCAGAGCAC GGCGGCCCGC

351 TCCCGGGGCC CCTTCCCGGG GGTCTCCACC GATGACAGCG CCGTGCCGCC

401 GCCGGGAGGG GCGCCCCATT TCGGGCACTA CCGGACGGGC GGCGGGGCCA

451 TGGGGCTGCG CAGCCGCTCG GTCAGCTCGG TGGCAGGCAT GGGCATGGAC

501 CCCAGCACGG CCGGGGGGGT GCCCTTTGGC CTCTACACCC CCGCCTCCCG

551 GGGCACCGGC GACTCCGAGA GGGCGCCCGG CGGCGGAGGG TCTGCGTCCG

601 ACTCCACCTA TGCCCATGGC AATGGTTACC AGGAGACGGG CGGCGGTCAC

651 CATAGAGACG GGATGCTGTA CCTGGGCTCC CGAGCCTCGC TGGCGGATGC

701 TCTACCTCTG CACATCGCAC CCAGGTGGTT CAGCTCGCAT AGTGGTTTCA

751 AGTGCCCCAT TTGCTCCAAG TCTGTGGCTT CTGACGAGAT GGAAATGCAC

801 TTTATAATGT GTTTGAGCAA ACCTCGCCTC TCCTACAACG ATGATGTGCT

851 GACTAAAGAC GCGGGTGAGT GTGTGATCTG CCTGGAGGAG CTGCTGCAGG

901 GGGACACGAT AGCCAGGCTG CCCTGCCTGT GCATCTATCA CAAAAGCTGC

951 ATAGACTCGT GGTTTGAAGT GAACAGATCT TGTCCGGAAC ACCCTGCGGA

1001 CTGACCTGCG GGCTTGCTTG CTGACTCCTC TCAAAGGGAC AGAGCGCCCC

1051 TGCTCCAGGG AGGAGGCTCA CCGGACCCTG GGGCAGAGCT GAGCTTGGGA

1101 CACCAGCGGG AACAGGGCAC CCCTTCTGCA CTGACTTCCA GATCATGGTT

1151 CTCCCTTCCT CCCTGAGGAC ACCAAATTGG ATGAGAGCAA GTTTGAGAGA

1201 AGAATGAATC AACTGCTATC CTTCCCCTCA CCCCTCAGCC CAGGAGGGAA

1251 AGGGCATTTT CTTTTTCATC TTTGAAAGGC ATTGTGGGTC TGTCTTTAAA

1301 GTGTTTACAA AAAAAAAA

BLAST Results

No BLAST result

Medlme entries

No Medlme entry

Peptide information for frame 3

ORF from 321 bp to 1001 bp; peptide length: 227 Category: similarity to known protein Classification: unclassified

1 MGGKQSTAAR SRGPFPGVST DDSAVPPPGG APHFGHYRTG GGAMGLRSRS

51 VSSVAGMGMD PSTAGGVPFG LYTPASRGTG DSERAPGGGG SASDSTYAHG

101 NGYQETGGGH HRDGMLYLGS RASLADALPL HIAPRWFSSH SGFKCPICSK

151 SVASDEMEMH FIMCLSKPRL SYNDDVLTKD AGECVICLEE LLQGDTIARL 201 PCLCIYHKSC IDSWFEVNRS CPEHPAD

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_9e22, frame 3

TREMBL:AF078823_1 product: "RING-H2 finger protein RHA2b"; Arabidopsis thaliana RING-H2 fmger protein RHA2b mRNA, complete eds., N = 1, Score = 111, P = 2.8e-06

TREMBL:AF078822_1 product: "RING-H2 finger protein RHA2a"; Arabidopsis thaliana RING-H2 fmger protein RHA2a mRNA, complete eds., N = 1, Score = 112, P = 6.6e-06

TREMBL:AC004138_14 gene: "T17M13.17"; Arabidopsis thaliana chromosome II BAC T17M13 genomic sequence, complete sequence., N = 2, Score = 123, P = 1.4e-05

PIR:T02286 hypothetical protein T13D8.23 - Arabidopsis thaliana, N = 1, Score = 142, P = 8.8e-08

>PIR:T02286 hypothetical protein T13D8.23 - Arabidopsis thaliana Length = 327

HSPs:

Score = 142 (21.3 bits), Expect = 8.8e-08, P = 8.8e-08 Identities = 24/57 (42%), Positives = 30/57 (52%)

Query: 166 SKPRLSYNDDVLTKDAGECVICLEELLQGDTIARLPCLCIYHKSCIDSWFEVNRSCP 222

S P + LT D +C +C+EE + G LPC IYHK CI W +N SCP Sbjct: 206 SLPSVKITPQHLTNDMSQCTVCMEEFIVGGDATELPCKHIYHKDCIVPWLRLNNSCP 262

Pedant information for DKFZphtes3_9e22, frame 3

Report for DKFZphtes3_9e22.3

[LENGTH] 227

[MW] 23782.62

[pi] 6.18

[HOMOL] PIR:T02286 hypothetical protein T13D8.23 - Arabidopsis thaliana 2e-08

[FUNCAT] 99 unclassified proteins [S. cerevisiae, YDR313c] 4e-06

[FUNCAT] 30.07 organization of endoplasmatic reticulum [S. cerevisiae, YOL013c]

0.001

[FUNCAT] 06.13 proteolysis [S. cerevisiae, YOL013c] 0.001

[PFAM] Zmc finger, C3HC4 type (RING finger)

[KW] Irregular

SEQ MGGKQSTAARSRGPFPGVSTDDSAVPPPGGAPHFGHYRTGGGAMGLRSRSVSSVAGMGMD PRD cccccccccccccccccccccccccccccccccccccccccccccccccceeeccccccc

SEQ PSTAGGVPFGLYTPASRGTGDSERAPGGGGSASDSTYAHGNGYQETGGGHHRDGMLYLGS PRD ccccccccccccccccccccccccccccccccccccccccccccccccccccccceeech

SEQ RASLADALPLHIAPRWFΞSHSGFKCPICSKSVASDEMEMHFIMCLSKPRLSYNDDVLTKD PRD hhhhhhhhceeecccccccccccccccccccchhhhhhhhhhhhcccccccccccccccc

SEQ AGECVICLEELLQGDTIARLPCLCIYHKSCIDSWFEVNRSCPEHPAD PRD cceeeeeecccccccccccccceeeeeeccchhhhhhhhcccccccc

(No Prosite data available for DKFZphtes3_9e22.3)

Pfam for DKFZphtes3_9e22.3

HMM_NAME Zmc fmger, C3HC4 type (RING finger)

HMM *CPICFcTFQlDyPWPFdePmMlPCgHsFCypCIrrW CPmC*

C IC L+++ D++ LPC+ ++ ++CI +W CP+ Query 184 CVIC LEELLQGDTIARLPCLCIYHKSCIDSWFEVNRSCPEH 224 DKFZphtes3_9ι20

group: testes derived

DKFZphtes3_9ι20 encodes a novel 205 ammo acid protein with similarity to human KIAA0336 gene.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application in studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, EST hits

Sequenced by DKFZ

Locus: /map="44.1 cR from top of Chrl7 linkage group"

Insert length: 2509 bp

Poly A stretch at pos. 2499, polyadenylation signal at pos. 2481

1 CTCGCCGAGA TGACCTGGGC ACCTCTGCGT TGAATCGGCA AATACTGATC

51 AAGCCGCATT TATTCTGCTC TCAGGAACTC TAAGTCTAGC AGAGAAGATG

101 AGGCGGTAGA AGTTCATCAA TGGCTTGGCT GGAGGACAAG CAAATTGAGG

151 ACATTGGCAA CGGAGTGATC AAAATGATAG ATCATGAGGC CTAAAATGAA

201 TAAGGAAAGA AGAGAAGTGG CAGAGGCTGA GAACAGAAAG AGAGGGTGGA

251 GGGGCTGTAA ATCTTGAAGA TTAGGGTATA ATATGAGTAT ATGGGTAAGA

301 ATTGGAAGAA TTGTGTAGGA GGCAGTAGTC AAAAAGTAGA AGCAGTTTGG

351 AAGAGTAGTT ACAAATATCA AGAGCCAGGT GGCTAAAAGG TGGAGCTATA

401 GGTCATTGAA GCTCAAGAAA CTGAGTCTCT AGGGCATTGG TTAAGTCATC

451 TGTCTAGACT TCAAAGTTGT CTAGGATGAT AATTCAGAAG ACTGATCTGT

501 GCCAAAGTCA CAGGTTTTTC ACGACTGAAA ACAACATAGC AAAATAAGCC

551 AAGATGTCTG TGGATCCAAT GACCTACGAG GCCCAGTTCT TTGGCTTCAC

601 GCCACAAACG TGCATGCTTC GGATCTACAT TGCATTTCAA GACTACCTAT

651 TTGAAGTGAT GCAGGCCGTT GAACAGGTTA TTCTGAAGAA GCTGGATGGC

701 ATCCCAGACT GTGACATTAG CCCAGTGCAG ATTCGCAAAT GCACAGAGAA

751 GTTTCTTTGC TTCATGAAAG GACATTTTGA TAACCTTTTT AGCAAAATGG

801 AGCAACTGTT TTTGCAGCTG ATTTTACGTA TTCCCTCAAA CATCTTGCTT

851 CCTGAAGATA AATGTAAGGA GACACCTTAT AGTGAGGAAG ATTTTCAGCA

901 TCTCCAGAAA GAAATTGAAC AGTTACAGGA GAAGTACAAG ACTGAATTAT

951 GTACTAAGCA GGCCCTTCTT GCAGAATTAG AAGAGCAAAA AATTGTTCAG

1001 GCCAAACTCA AACAGACGTT GACTTTCTTT GATGAGCTTC ATAATGTTGG

1051 CAGAGATCAT GGGACTAGTG ATTTTAGGGA GAGTTTAGTA TCCCTGGTTC

1101 AGAACTCCAG AAAACTACAG AACATTAGAG ACAATGTGGA AAAGGAATCG

1151 AAACGACTGA AAATATCTTA ATTGCTCAGT AGTCAAAAGG AGGAGCCTGT

1201 CAAAAAGTAG AATCATAAGG ACTGTTCAAA CCATAAGGAC TGTTCAAATC

1251 ATACCAGTGA CTGTTCAAAC CAACCATACT TTTTATTAGA TTTGCTTTGT

1301 CAACTCTTTC TTGTATTCTG TGTTTTCCTC TTTTTTGGTC CACTTTGCTG

1351 AGGTATGAAG TGTACTACTT TGAACTAGGC TGAAGCATCT GAGTCTTCTA

1401 ATAAGTGGGA AGGGATCCAA CAAAGAAGCC ATGACCAGTT AAAGATATTT

1451 GCAGAGTTAC ACCTTGGTCA TAAGTCCTTT GTGACCTTGA TTATTTTGGC

1501 TTACTCTTTG GATGAGACCA GACAAGAAAA GGATTAAACG GGTGGCTCCT

1551 TTAATATTAT TATTATTGTT TTTGAGACAA GGTCCCTTTC TGTCACCCAG

1601 GTTAGAGTAG ATTTCAGTGG CACAATCTTG GCTCACTGCA ACCTCTGTGT

1651 CCTGGGCTCA AGTGATCCTC CTGCCTCAGC CTCCCAAGTA GCTAGGACCA

1701 CAGGTGCGTG TCACCATGCT TGGCTAATTT TTTTGCAGAA ACGAGGCCTC

1751 ACTATATTGT CCAGGCTGAG TGGCTCTTTT ATTAACCAGT CATTACACTG

1801 CGGAACAGCC AACATAGAGT ACTTGCTCTC GTCCTGTGAA TTTTCTTTCA

1851 TGAGGGAGTC AATATGTAGT GGAAAGAAGC ATGTAGCAAA AAAGACAACC

1901 TTGATCTTTA ATAAAAAAGA AGTTGGTTTA TTTCCAAAAT AAATCCCCTG

1951 ACAAAAAACC TGGTGATGTT AAGCAATTGA CTGTCTTAGA GTCCAGCAGA

2001 AGACCTTAGA CAAAAAAAGC AGAACCCACT GGAGTAGAAA AGGAAGCATG

2051 TAGCATATAC TCAGTAGTGA AATTTAATTT TACTGACTGT TAGGTATCTA

2101 TGCCAATTTG TTTTCATACT TCAGTTGGTT TTGGAATCTG CCTTATACCT

2151 AATATTTATT TATTCACACT CATAAGCATC AAATATTTAA TGCCCTCAGT

2201 GGGAAATTTG TGTTTAAACT CAATGGAATC TAATATTTCT TTATGTCGTT

2251 AGTCCCTGTA AAATGTTAGG TCACCCAAGG AAAGGGGAGA AATAGCAATG

2301 GTTGTTCCTA AGGTATTGCT TGCCCTCCAT GTCTTCCTAA AGAGCAGAAC

2351 TTGGAGTTTC TCCTTTATGT AGAGAAGAAG TAACTTAGGG TGTATTTGCA

2401 ATGAAATATT CATAGATATT GAAAGCTTGT GTTTACATGA AATATGTTTA

2451 TTATCAAGAA GTCCTTTTTC CAATTCTGTA CATTAAATAT ATGTGTTTTA

2501 AAAAAAAAA

BLAST Results Entry AC004148 from database EMBL:

Homo sapiens chromosome 17, clone HCIT524C5, complete sequence.

Score = 5245, P = O.Oe+00, identities = 1049/1049

3 exons

Entry HS556361 from database EMBL: human STS TIGR-A003N29.

Score = 1005, P = 1.3e-39, identities = 201/201

Entry HSG043 from database EMBL: human STS SHGC-36031. Score = 955, P = 2.8e-37, identities = 205/215

Medlme entries

No Medline entry

Peptide information for frame 2

ORF from 554 bp to 1168 bp; peptide length: 205 Category: putative protein Classification: no clue

1 MSVDPMTYEA QFFGFTPQTC MLRIYIAFQD YLFEVMQAVE QVILKKLDGI

51 PDCDISPVQI RKCTEKFLCF MKGHFDNLFS KMEQLFLQLI LRIPSNILLP

101 EDKCKETPYS EEDFQHLQKE IEQLQEKYKT ELCTKQALLA ELEEQKIVQA

151 KLKQTLTFFD ELHNVGRDHG TSDFRESLVS LVQNSRKLQN IRDNVEKESK

201 RLKIS

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_9ι20, frame 2

TREMBLNE :HSAB2334_1 gene: "KIAA0336"; Human mRNA for KIAA0336 gene, complete eds., N = 1, Score = 107, P = 0.0081

>TREMBLNEW:HSAB2334_1 gene: "KIAA0336"; Human mRNA for KIAA0336 gene, complete eds.

Length = 1,583

HSPs:

Score = 107 (16.1 bits), Expect = 8.2e-03, P = 8.1e-03 Identities = 42/140 (30%), Positives = 76/140 (54%)

Query: 65 EKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEED FQHLQKE 120

EK CF+K H +NL +EQ +L R ILL +D ++P + D + L+++ Sbjct: 796 EKEKCFIKEH-ENLKPLLEQK—ELRDRRAELILL-KDSLAKSPSVKNDPLSSVKELEEK 851

Query: 121 IEQLQE—KYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESL 178

IE L++ K K E K L+A ++ +K + + K+T T +EL ++ + S+ Sbjct: 852 IENLEKECKEKEEKINKIKLVA-VKAKKELDSSRKETQTVKEELESLRSEK—DQLSASM 908

Query: 179 VSLVQNSRKLQNIRDNVEKESKRLKI 204

L+Q + +N+ EK+S++L + Sbjct: 909 RDLIQGAESYKNLLLEYEKQSEQLDV 934

Pedant information for DKFZphtes3_9ι20, frame 2

Report for DKFZphtes3_9ι20.2

[LENGTH] 205

[MW] 24140.13

[pi] 5.51

[KW] All_Alpha

[KW] COILED_COIL 18.05 % SEQ MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVILKKLDGIPDCDISPVQI

PRD cccccchhhhhhcccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccc

COILS

SEQ RKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEEDFQHLQKE

PRD cccchhhhhhhcccccchhhhhhhhhhhhhhhcccceeeccccccccccchhhhhhhhhh

COILS CCCCCCCCCC

SEQ IEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESLVS

PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccchhhhhhhh

COILS CCCCCCCCCCCCCCCCCCCCCCCCCCC

SEQ LVQNSRKLQNIRDNVEKESKRLKIS

PRD hhcccchhhhhhhhhhhhhhhcccc

COILS

(No Prosite data available for DKFZphtes3_9 20.2) (No Pfam data available for DKFZphtes3 9ι20.2)

DKFZphtes3_9k22

group: testes derived

DKFZphtes3_9k22 encodes a novel 304 ammo acid protein with partial similarity to X. leavis katanin p80.

No informative BLAST results; No predictive prosite, pfam or SCOP motife.

The new protein can find application m studying the expression profile of testis-specific genes . similarity to C-terminus of katanin p80

Sequenced by DKFZ

Locus : unknown

Insert length: 2676 bp

Poly A stretch at pos. 2665, no polyadenylation signal found

1 CTCTCTAGGC TGCCGGGCGC TGGTCGTCAG CGCCGAGGCT GGGCTGAGGC

51 GCCGCGGTAC CATGAGGCGC CGGTACTTAA GAGATTATGG CATCAGAAAC

101 CCACAATGTT AAAAAACGGA ACTTTTGTAA TAAGATTGAG GATCATTTCA

151 TTGATCTTCC TAGAAAAAAG ATCTCTAATT TCACTAATAA GAACATGAAG

201 GAGGTTAAGA AATCTCCAAA ACAGTTGGCT GCTTACATAA ATAGAACAGT

251 TGGACAAACT GTGAAAAGCC CAGATAAACT TCGTAAAGTG ATCTATCGCA

301 GAAAGAAAGT TCATCATCCC TTTCCAAATC CTTGTTACAG AAAAAAACAG

351 TCCCCTGGAA GTGGGGGCTG TGACATGGCA AATAAAGAAA ATGAACTGGC

401 TTGTGCAGGC CACCTGCCTG AAAAATTACA CCATGATAGT CGAACATATT

451 TGGTTAACTC CAGTGATTCT GGTTCTTCAC AGACAGAAAG CCCATCATCA

501 AAATATAGTG GGTTTTTTTC TGAGGTTTCT CAGGACCATG AAACAATGGC

551 CCAAGTTTTG TTCAGCAGGA ATATGAGATT GAATGTAGCT TTAACTTTCT

601 GGAGAAAGAG AAGTATAAGT GAACTTGTAG CTTATTTGTT GAGGATAGAA

651 GATCTTGGCG TTGTGGTAGA TTGCCTTCCT GTGCTCACCA ATTGTTTACA

701 GGAAGAAAAA CAATATATCT CACTTGGCTG CTGTGTTGAC TTGTTGCCTC

751 TAGTAAAGTC ACTACTTAAA AGCAAATTTG AAGAATATGT TATAGTTGGT

801 TTAAACTGGC TTCAAGCAGT CATTAAAAGG TGGTGGTCAG AACTATCATC

851 CAAAACAGAA ATTATAAATG ATGGAAATAT TCAAATTTTA AAACAACAAT

901 TAAGTGGATT ATGGGAACAG GAAAACCATC TTACTTTGGT TCCAGGATAT

951 ACTGGTAATA TAGCTAAGGA TGTAGATGCT TATTTATTAC AGTTACATTG

1001 AGAGATTTCA TCTACTAAAG AGCATTTGGT TTTTCAAAAC ATCCCTGAAC

1051 TGTATAATTT ACAAAAAAAA AAGTCTCGTC TGAGAACTGT GAACTGTGGA

1101 AGAAATCAAA ACTATTTTTT CTTTTAAAAA GCCACGTAAT GAAACCACTA

1151 ATGAAATCCC AGCAATCTGC TTCACATTGA AGTGGAAAAA TATCCAAAAG

1201 GAGCAGCTTC AATTTCATTG AGGTGAAAGT GCACTATGAA GATTGTTCAC

1251 CTTTGCTGCA TTTGGGAGTT ATATGGTTAT TTGGTAACAT TAAGAACTAC

1301 TGGATTTTAA TGCAATCCTG CATAAAAATA TAATTTATAC TATGTGAAAA

1351 AATAAGACAG GACTTACCAC TAGGAACCAC CAAGACCAAT CATCATTAAC

1401 TTTTTTAAGA TTGTGTTTTA TTAAAAAAAA AAAACACTTA AATGTGTGCA

1451 GCTATTTTCT TATGTTGAAA AGACTGAAAG TTTAAAACAT GAAAAAAATC

1501 AATATTAAAC ATTTTTTGTT CACACTGAGA TACTGTGTAT GTAAAATGCC

1551 TTAATTATTA ATAAGCCAAT GTGTTATGAT ACCAATATCT GTTTTAAAAA

1601 ACTAAAACCA ACCATGCTTC TGGCATGATA AAATCATGGA ATTAAATCAG

1651 GGGTTTACAT TCTTGTAGAG TGTTCTTGAA ACACTCTCTG CACCATTTTT

1701 AAAACTTGAG AATAGTTTTA GTATCTCTGA TATTTTTTGC CAGAATCATC

1751 ATGTCATGTA TGAATGTGTT ATCCCTATCT AAGGAAAAAG GTGAATATGT

1801 TTTTGTATGA ATGTTTAACT GGAAATGTCC ATGGACTTGG CTAATTTATA

1851 TTTACTTTTT ATTGTACATA GATTTCTAAT ATTTTTCATT CCTGTATCAT

1901 TTAAACTTCC TTCATTTGAG TAAATTCACT AAATATTTCT ATTTTTTTGC

1951 TTTTTTAAAT TCTGATTTTA TATGAATTCT AATTCTTTTT CACTACATAT

2001 GTTTTAAAGA GTTACATACA GTGATTTAGA ATGGTTTACA GTTAATGCTG

2051 ATCTTGTATT TTAAATTCCA ACACTTTGTG TCACTACCTC CTCTAATGGT

2101 TAGTATGATA TGCTAGCAGA CTGTATGAGG TCTTTTTTTA AAATACCACT

2151 TTTAGTGTCA GTGAACCAAA TTCTGGAATG TCTTAACAGC TCTAAATCTT

2201 ACTTGTCTTG AAAATGATTG GGGTTTAATA CCACTGCTGG TGGTTCACAC

2251 ATCATCCCAT CCTTAATATG CCTGACAGGC ATCTGAGCAA AGGTTTTTAG

2301 TAATTGAATT TCTCTGCAGT AGTCCTTCAA GCACTTGAAT GTAAACCTTT

2351 AGCATTTATT CGTTTAATGA CTACTGATAC GAATCTCAAG CAGATTTCTT

2401 GCTCTTAAAA GTTATGTTTC ACTGAGTTCT GGTTTTGTGT AGCTATATTT

2451 TATATAGCTA GATATTCCTC ACAGTGAACA TGAATTGTAA TAATTGGTTA

2501 TTTCCTTAAG TCTTTAGATT ATAATAATTT CAGATTATTG CACGTCTGTG

2551 ATTTGAGAGG TGAGTTATTT AAGAGGCCAG TTTTCAGGAC ATGGGAATTT

2601 GAATTGTAAA CCTGTTATCT CTGTGAAACT TTTAACATGA TAAAATATAA

2651 CCTTTCTTTG TGCTTAAAAA AAAAAA BLAST Results

Entry HS541354 from database EMBL: human STS WI-11840. Score = 1267, P = 7. le-50, identities = 271/281

Medline entries

98227670:

Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-contaιnιng subunit.

Peptide information for frame 3

ORF from 87 bp to 998 bp; peptide length: 304 Category: similarity to known protein Classification: unclassified

1 MASETHNVKK RNFCNKIEDH FIDLPRKKIS NFTNKNMKEV KKSPKQLAAY 51 INRTVGQTVK SPDKLRKVIY RRKKVHHPFP NPCYRKKQSP GSGGCDMANK 101 ENELACAGHL PEKLHHDSRT YLVNSSDSGS ΞQTESPSSKY SGFFSEVSQD 151 HETMAQVLFS RNMRLNVALT FWRKRSISEL VAYLLRIEDL GVVVDCLPVL 201 TNCLQEEKQY ISLGCCVDLL PLVKSLLKSK FEEYVIVGLN WLQAVIKRWW 251 SELSSKTEII NDGNIQILKQ QLSGLWEQEN HLTLVPGYTG NIAKDVDAYL 301 LQLH

BLASTP hits

No BLASTP hits available

Alert BLASTP hits for DKFZphtes3_9k22, frame 3

TREMBL: AF056021_1 product: "p80 katanin"; Xenopus laevis p80 katanin mRNA, partial eds., N = 1, Score = 146, P = 1.2e-07

TREMBL:AF052432_1 product: "katanin p80 subunit"; Homo sapiens katanin p80 subunit mRNA, complete eds., N = 1, Score = 150, P = 1.2e-07

TREMBL: AF052433_1 product: "katanin p80 subunit"; Strongylocentrotus purpuratus katanin p80 subunit mRNA, complete eds., N = 2, Score = 146, P = 4.2e-07

>TREMBL: AF052432_1 product: "katanin p80 subunit"; Homo sapiens katanin p80 subunit mRNA, complete eds. Length = 655

HSPs:

Score = 150 (22.5 bits), Expect = 1.2e-07, P = 1.2e-07 Identities = 35/105 (33%), Positives = 55/105 (52%)

Query: 145 SEVSQDHETMAQVLFSRNMRLNVALTFWRKRSISELVAYLLRIEDLGVVVDCLPVLTNCL 204

S++ + H+TM VL SR+ L+ W I V + I DL VVVD L N + Sb_jct: 489 SQIRKGHDTMCVVLTΞRHKNLDTVRAVWTMGDIKTSVDSAVAINDLSVVVDLL NIV 544

Query: 205 QEEKQYISLGCCVDLLPLVKSLLKSKFEEYVIVGLNWLQAVIKRW 249

++ L C +LP ++ LL+SK+E YV G L+ +++R+ Sbjct: 545 NQKASLWKLDLCTTVLPQIEKLLQSKYESYVQTGCTSLKLILQRF 589

Pedant information for DKFZphtes3_9k22, frame 3

Report for DKFZphtes3_9k22.3

[LENGTH] 304

[MW] 34767.24

[pi] 9.18

[KW] All Alpha [KW] LOW_COMPLEXITY 3.95 %

SEQ MAΞETHNVKKRNFCNKIEDHFIDLPRKKISNFTNKNMKEVKKSPKQLAAYINRTVGQTVK

SEG

PRD ccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhccccc

SEQ SPDKLRKVIYRRKKVHHPFPNPCYRKKQSPGSGGCDMANKENELACAGHLPEKLHHDSRT

SEG

PRD ccchhhhhhhhhhhcccccccccccccccccccccccccchhhhhhccccccccccccce

SEQ YLVNSSDSGSSQTESPSSKYSGFFSEVSQDHETMAQVLFSRNMRLNVALTFWRKRSISEL

SEG

PRD eeecccccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

SEQ VAYLLRIEDLGVVVDCLPVLTNCLQEEKQYISLGCCVDLLPLVKSLLKSKFEEYVIVGLN

SEG xxxxxxxxxxxx

PRD hhhhhhhhhcceeeeeeccchhhhhhhhceeeccceeeehhhhhhhhhhhheeeeeeehh

SEQ WLQAVIKRWWSELSSKTEIINDGNIQILKQQLSGLWEQENHLTLVPGYTGNIAKDVDAYL

SEG

PRD hhhhhhhhhhhhcccceeeeccccccccccccchhhhhhhhhhccccccccchhhhhhhh

SEQ LQLH

SEG ....

PRD hccc

(No Prosite data available for DKFZphtes3_9k22.3) (No Pfam data available for DKFZphtes3_9k22.3)

Localization of expressed proteins

KΩ KΩ

KΩ KΩ 00

KΩ KΩ KΩ

CD O O

o o

o o l\3

o o to

o

CD

CD O cn

Table of cDNA clones and related data

Group: cell cycle

hfbr2_16gl8 Similarity to KIAA0797 and yeast Novel protein with similarities to S. pombe SPAC17A5.07C and the S. cerevisiae Cell cycle Smt4p Smt4p suppressor of MIF2 gene.; involved in centromer organisation hfbr2 2kl4 Strong similarity to human N₃₃ New tumour suppressor gene Cell cycle tumour suppressor gene htes_{3 3}5b4 Human M-phase phosphoprotein-1 The novel protein is C-terminal identical to human M-phase phosphoprotein-1, which Cell cycle is expressed and phosphorylated in the metaphase . Therefore the novel protein seems to be involved in the mitotic spindle during cell division. htes3_35p22 Strong similarity to oncogene 1 Oncogene Cell cycle (tre-2 locus) htes3_7j3 Related to the C-TAKl Cdc25C Cdc25C is a protein kinase that controls entry into mitosis by dephosphorylation Cell cycle associated protein kinase of Cdc2. Cdc25C function is regulated by phosphorylation, too. Serine 216 phosphorylation of Cdc25C mediates the binding of 14-3-3 protein to Cdc25C. C-TAKl (Cdc twenty- five htes3_7pl0 Strong similarity to XPMC2 protein XPMC2 of xenopus rescues several different yeast mitotic catastrophe mutants Cell cycle defective in Weel/Mikl kinase function. hutel 20mll Similarity to suppressor protein Suppressor regulator of protein phosphatase-1 Cell cycle sds22

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Group cell structure and motility

Group Differentiation/Development

hfbr2 2dl5 Mus musculus testis-specific Y- TSPY is believed to function in early spermatogenesis and is a candidate for GBY, Differentiat encoded-like protein (Tspyll) the putative gonadoblastoma-inducing gene on the Y-chromosome ion/Developm ent htes3 35e21 Similarity to interleukin-7 New interleukin Differentiat precursor ion/Developm ent hutel 2h3 Strong similarity to mouse E25 and Homolog is marker for chondro-osteogenic dif erentiation Differentiat gallus E3-16 ion/Developm ent

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Group kidney derived

hfkd2_l 9 Strong similarity to XLCL2 No informative BLAST results, No predictive prosite, pfam or SCOP motive Kidney protein, African clawed frog derived hfkd2 24e23 Unknown No informative BLAST results, No predictive prosite, pfam or SCOP motive Kidney derived hfkd2 46a6 Unknown No informative BLAST results, No predictive prosite, pfam or SCOP motive Kidney derived hfkd2 46bl0 Similarity to C elegans F25B5 3 No informative BLAST results, No predictive prosite, pfam or SCOP motive Kidney derived hfkd2 46dl3 Weak similarity to KE03 protein Contains a RGD site, Kidney No informative BLAST results, No predictive prosite, pfam or SCOP motive derived hfkd2 4b6 Similarity to Homo sapiens clone No informative BLAST results. No predictive prosite, pfam or SCOP motive Kidney 25003 partial CDS. derived hfkd2 4c8 Similarity to KIAA0549 and HAP1 No informative BLAST results, No predictive prosite, pfam or SCOP motive Kidney (Huntingtin-associated protein-1) derived

Group mammary carcinoma derived

hmcfl 1C23 Unknown ;No informative BLAST results; No predictive prosite, pfam or SCOP motive Mammary

Carcinoma derived hmcfl_lgl3 Similarity to KIAA0766; very weak No informative BLAST results; No predictive prosite, pfam or SCOP motive Mammary similarioty to transposases Carcinoma

derived

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Group Nucleic acid management

hfbr2 23bl0 Similarity to rat RNA helicase RNA helicase Nucleic Acid HEL117 Management hfbr2 3cl8 Strong similarity to RNA helicase DEAD-box Nucleic Acid and RNA-dependent ATPase from the Management DEAD box family hfbr2 64al5 Similarity to inorganic Inorganic pyrophosphatase Nucleic Acid pyrophosphatases (unspliced) Management hfbr2 6ol7 Strong similar to RNA helicases RNA helicases Nucleic Acid Management hfbr2 72bl8 Similarity to DNA damage induced Similar to dinP of E. coli, yqjH of B. subtilis, dinP of M. tuberculosis and Nucleic Acid genes T19K24.15 of A. thaliana. The dinB/P pathway is a second SOS-pathway in E. coli Management hfbr2 72112 Similarity to YDR126W DNA binding protein Nucleic acid managment hfbr2 82i24 Strong similarity to DEAD-box Dead-box helicase Nucleic Acid subfamily ATP-dependent helicase Management htes3 14h21 Strong similarity to RNA helicases RNA helicase Nucleic Acid Management htes3_15j3 Similarity to YGR276C, a Rnase H Nucleic Acid ribonuclease H of S . cerevisiae . Management htes₃ 20ml8 Similarity to the S. cerevisiae The novel protein contains a leucine zipper and a Prosite mitochondrial energy Nucleic Acid mitochondrial carrier protein transfer proteins signature. It is member of a family of substrate carrier Management RIM2. proteins which are found in the inner mitochondrial membrane and are involved in energy transfer. htes₃_22g2 KIAA0829 is shorter, nearly Involved in TATA box binding complex Nucleic Acid identical to rat TIP120 Management htes₃ 2ml8 Nearly identical to mouse Dhml Multifunctional nuclease/exoribonuclease Nucleic acid management htes3_7p9 Similarity to nuclear domain 10 Transcription control Nucleic Acid protein NDP52 Management htes3 8ml0 Strong similarity to The poly (A) -binding protein (PABP) binds to the messenger (mRNA) 3 '-poly (A) tail Nucleic Acid polyadenylate-binding proteins. found on most eukaryotic mRNAs and together with the poly (A) tail has been Management implicated in governing the stability and the translation of mRNA. hutel 1811 Strong similarity to S. cerevisiae Mitochondrianl Ribosomal S40 protein Nucleic Acid YHR148w Management

Group testis associated

htes3 2m20 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motife. Testes associated htes3 2n9 Very weak similarity to Homo No informative BLAST results; No predictive prosite, pfam or SCOP motife. Testes sapiens PAC clone DJ0771P04 from associated 7qll.21-qll.23. htes3 30f4 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motife. Testes associated htes₃_35g6 Strong similarity to R27216_l No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes3 35n24 Unknown Contains an IG_MHC pattern Testes

No informative BLAST results; No predictive prosite, pfam or SCOP motive associated htes3_35pl7 Similarity to S. cerevisiae VAC8 No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes and beta-Catenin, but contains no associated a adillo motifes htes3 4b4 Rattus norvegicus late gestation No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes lung protein 1 associated htes3 4fl7 Similarity to KIAA03₃₃ No informative BLAST results,- No predictive prosite, pfam or SCOP motive Testes Methyl-CpG binding protein,- does associated not contain such a motife. htes3 4ol9 Similarity to mucin No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes3_50j4 Unknown, prolin rich protein No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated o htes₃ 50n2₃ Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes cn associated htes3 50n6 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes₃ 6b21 Similarity to KIAA0256 No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes3 6dl6 WUGSC:H_DJ1185I07.2, differences No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes to genmodel associated htes3 72kll Similarity to S.pombe hypothetical No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes repeat-containing protein associated htes3 7dl7 Similarity to KIAA0454 No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes3_7j8 WUGSC :H_DJ1159O04.1 similarity to No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes YBL104p associated htes3_8gll Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes3_8g5 KIAA087, alternative spliced No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes3_8p7 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes3 9e22 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes

associated htes3 9i20 Unknown No informative BLAST results,- No predictive prosite, pfam or SCOP motive Testes associated htes3 9k22 Similarity to C-terminus of No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes katanin p80 associated

Group transmembrane proteins

hfbr2 16il2 Similarity to Fugu rubripes PUT2 1 transmembrane domain Transmembran

No informative BLAST results; No predictive prosite, pfam or SCOP motife. e protein hfbr2 16112 Similarity to gallus putative 1 transmembrane domain Transmembran trans embranee protein E3-16 No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfbr2 22hl3 Similarity to Drosophila 1 transmembrane domain Transmembran melanogaster EG:39E1.3. No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfbr2 2bl7 Similarity to Drosophila 3 transmembrane domains Transmembran hypothetical 3OK protein No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfbr2 2dl7 Unknown 1 transmembrane domain Transmembran

No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfbr2 64k24 Similarity to several proteins 5 transmembrane regions. Transmembran

No informative BLAST results; No predictive prosite, pfam or SCOP motife. e protein hfbr2 82C20 Similarity to C. elegans D1007.5 7 transmembrane domains Transmembran

No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfbr2 82el7 Similarity to C. elegans "R01B10.5" 6 transmembrane domains Transmembran

No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfbr2_82gl4 Unknown proline rich protein 1 transmembrane domain Transmembran

No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfkd2 24al5 Similarity to C. elegans R07G3.8 1 transmembrane domain Transmembran

No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfkd2 3il3 Similarity to A. thaliana YUP8H12.2 3 transmembrane domains Transmembran

No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfkd2 4mll Weak similarity to YMR034C 4 transmembrane domains Transmembran

No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hmcfl lall Similarity to YDR255C and 1 transmembrane domain Transmembran SPBC29A3.03C No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hmcfl lel5 Similarity to D-XYLOSE TRANSPORTER Transporter; Transmembran

9 transmembrane domains e protein

No informative BLAST results; No predictive prosite, pfam or SCOP motive htes3 15c6 Unknown 1 transmembrane domain Transmembran

No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein htes3 2ol3 Partial similarity to the IL-17 1 transmembrane domain Transmembran receptor . No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein htes3 27k4 Strong similarity to C. elegans Contains a leucine zipper Transmembran K07H8.2/ZK185.2 10 transmembrane domains e protein

No informative BLAST results; No predictive prosite, pfam or SCOP motive; htes3 2hl Similarity to C. elegans C13F10.5 1 transmembrane domain Transmembran

No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein htes₃ 35k24 Unknown 5 transmembrane domains Transmembran

No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hutel 19fl9 Similarity to mouse P24 protein 2 transmembrane domains Transmembran

No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hutel 24cl9 Unknown 1 transmembrane domain Transmembran

No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein

Group Brain derived

hfbr2 64cl6 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 64C4 Similarity to A. thaliana T08I13.5 No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 64h6 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 64i20 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 64ol6 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 6al7 Weak similarity to finger protein No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain zfOCl derived hfbr2 6i20 Similarity to ribosomal protein No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain L15 precursor, mitochondrial derived hfbr2 71O20 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 72dl3 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 72ml6 Similarity to C. elegans H14A12.3 No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 72nl2 Strong similarity to rat No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain Ganglioside expression factor derived

GEF-2) but even higher identity with C. elegans putative protein Identities = 91/116 (78%) hfbr2 78dl3 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 78n23 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 7a24 DKFZphfbr2_7a24.1 similarity to C- Only c-terminus homolog; contains no kinase domain; Brain terminus of TGF-beta-activated No informative BLAST results; No predictive prosite, pfam or SCOP motive derived kinase hfbr2 7e22 Similarity to cytochrome b561 No heme domain but a c may helix loop helix signature Brain

No informative BLAST results; No predictive prosite, pfam or SCOP motive derived hfbr2_7j4 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 82ml6 Very weak similarity to A. thaliana No informative BLAST results; No predictive prosite, pfam or" SCOP motive Brain F28A23.140 derived

Group Intracellular Transport and Trafficking

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KΩ

Group signal transduction

hutel 22el2 Strong similarity to S. cerevisiae The Drosophila cni and mammalian proteins cornicon are part of a signal Signal YGL054C and cornichon transduction pathway involving hte EGF-receptor transduction

Group Metabolism

Group transcription factors

hfkd2 46kl9 Strong similarity to pterin-4- Dcoh is a bifunctional protein, complexed with biopterin. It serves as Transcriptio alpha-carbinolamine dehydratase dimerization cofactor of hepatocyte nuclear factor-1 and catalyzes the dehydration n factor of the biopterin cofactor of phenylalanine hydroxylase hfkd2 47a4 Similarity to zinc fingers New putative transcription factor with one C2H2 zinc fingers. Transcriptio n factor htes3 2el2 Similarity to finger proteins Transcription factor with three C2H2 zinc fingers. Additionally, a cytochrome C Transcripton family heme-binding site signature is present in the protein factros htes₃_21j l5 3 strong similarity to "NY-CO-33" Transcription factor Transcriptio n factors htes3 17nl2 Nearly identical to mouse SOX-LZ SOX-LZ, related to SRY and HMG-box-Proteins Transcriptio n factors hutel 18il9 Similarity to transcription factor The SREBP-2 protein is cleaved to release soluble NH2-terminal that enter the Transcriptio SF3 nucleus and activate genes encoding the low density lipoprotein receptor and n factor enzymes of cholesterol synthesis; a lim domain; shows similarity to the common sunflower transcripti hutel li2 Similarity to Dictostelium myosin Zn- finger protein Transcriptio heavy chain kinase n factor

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Group uterus associated ftthiJΛ hutel 17k7 Similarity to HPBRII-4 MRNA No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus associated hutel 18cl2 Similarity to candidate tumor No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus suppressor p₃₃INGl associated hutel 18i4 Weak similarity to C. elegans No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus D2085.2 associated hutel_19gl9 Partial similarity to bovine No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus elastin fragment associated hutel_19jll Strong similarity to KIAA02₃l, No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus similarity to ras binding protein associated Sur8 hutel 22n2 Similar to F46F6.1 No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus associated hutel 21dl5 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus associated hutel 22o2 Similarity to S. ombe SPBC3E7.03c No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus associated hutel_23gll Similarity to SPAC31G5.12C and No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus Maflp associated

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Prosite Key

NAME' N-glycosylation site. CONSENSUS. N-{P}-[ST]-{P}

NAME Glycosaminoglycan attachment site. CONSENSUS: S-G-x-G.

NAME Tyrosine sulfation site

NAME: cAMP- and cGMP-dependent protein kinase phosphorylation site CONSENSUS: [RK](2)-x-[ST].

NAME: Protein kinase C phosphorylation site CONSENSUS: [ST]-x-[RK].

NAME: Casein kinase II phosphorylation site CONSENSUS- [ST]-x(2)-[DE].

NAME: Tyrosine kinase phosphorylation site CONSENSUS. [RK]-x(2,3)-[DE]-x(2,3)-Y

NAME: N-myπstoylation site.

CONSENSUS. G-{EDRKHPFYW}-x(2)-[STAGCN]-{P}

NAME: Amidation site CONSENSUS: x-G-[RK]-[RK].

NAME: Aspartic acid and asparagine hydroxylation site CONSENSUS C-x-[DN]-x(4)-[FY]-x-C-x-C

NAME: Vitamin K-dependent carboxylation domain

CONSENSUS' x( 12)-E-x(3)-E-x-C-x(6)-[DEN]-x-[LIVMFY]-x(9)-[FYW]

NAME. Phosphopantetheine attachment site.

CONSENSUS [DEQGSTALMKRH]-[LrVMFYSTAC]-[GNQ]-[ IVMFYAG]-[DNEKHS]-S-[LIVMST]-

CONSENSUS. {PCFY}-[STAGCPQLIVMF]-[LIVMATN]-[DENQGTAKRHLM]-[ IVMWSTA]-[LIVGSTACR]-

CONSENSUS x(2)-[LIVMFA).

NAME- Acyl earner protein phosphopantetheine domain profile

NAME: Prokaryotic membrane lipoprotein lipid attachment site

CONSENSUS {DERK}(6)-[LIVMFWSTAG](2)-[LIVMFYSTAGCQ]-[AGS]-C .

NAME: Prokaryotic N-terminal methylation site

CONSENSUS: [KRHEQSTAG]-G-[FY IVM]-[ST1-[LT1-[LIVP]-E-[LIVMF STAG](14).

NAME: Prenyl group binding site (CAAX box) CONSENSUS: C-{DENQ}-[LIVM]-x> .

NAME: Protein splicing signature.

CONSENSUS. [DNEG]-x-[LIVFA]-[LrVMY]-[LVAST]-H-N-[STC]

NAME: Endoplasmic reticulum targeting sequence CONSENSUS: [KRHQSA]-[DENQ]-E-L > .

NAME Microbodies C-terminal targeting signal. CONSENSUS: [STAGCN]-[RKH]-[LIVMAFY] > .

NAME: Gram-positive cocci surface proteins 'anchoring' hexapeptide CONSENSUS: L-P-x-T-G-[STGAVDE].

NAME: Bipartite nuclear targeting sequence.

NAME: Cell attachment sequence. CONSENSUS: R-G-D.

NAME ATP/GTP-binding site motif A (P-loop). CONSENSUS. [AG]-x(4)-G-K-[ST].

NAME Cyclic nucleotide-binding domain signature 1.

CONSENSUS [LIVM]-[VIC]-x(2)-G-[DENQTA]-x-[GAC]-x(2)-[LIVMFY]( )-x(2)-G. NAME Cyclic nucleotide-binding domain signature 2

CONSENSUS [LIVMF]-G-E-x-[GAS]-[LIVM]-x(5, 1 l)-R-[STAQ]-A-x-[LIVMA]-x-[STACV]

NAME cAMP/cGMP binding motif

NAME EF-hand calcium-binding domain

CONSENSUS D-x-[DNS]-{ILVFYW}-[DENSTG]-[DNQGHRK]-{GP}-[LIVMC]-[DENQSTAGC]-x(2)-

CONSENSUS [DEHLIVMFYW]

NAME Actinin-type actin-binding domain signature 1 CONSENSUS [EQ]-x(2)-[ATV]-[FY]-x(2)-W-x-N

NAME Actimn-type actin-binding domain signature 2

CONSENSUS [LIVM]-x-[SGN]-[LIVM]-[DAGHE]-tSAG]-x-[DNEAG]-[LIVM]-x-[DEAG]-x(4)-

CONSENSUS [LIVM]-x-[LM]-[SAG]-[LIVM]-[LIVMT]-W-x-[LIVM](2)

NAME Anaphylatoxin domain signature

CONSENSUS [CSH]-C-x(2)-[GAP]-x(7,8)-[GASTDEQR]-C-[GASTDEQL]-x(3,9)-[GASTDEQN]-x(2>-

CONSENSUS [CE]-x(6,7)-C-C

NAME Anaphylatoxin domain profile

NAME Apple domam

CONSENSUS C-x(3)-[ IVMFY]-x(5)-[LIVMFY]-x(3)-[DENQ]-[LIVMFY]-x(10)-C-x(3)-C-T-

CONSENSUS x(4)-C-x-[LrVMFY>F-x-[FY]-x(13,14)-C-x-[LIVMFYHRK]-x-[ST]-x(14,15)-

CONSENSUS S-G-X-[STJ-[ ΓVMFY] X(2)-C

NAME Band 4 1 family domain signature 1

CONSENSUS W-[LIV]-x(3)-[KRQ]-x-[LIVM]-x(2)-[QH]-x(0,2)-[ IVMF]-x(6,8)-[LIVMF]-

CONSENSUS x(3,5)-F-[FY]-x(2)-[DENS]

NAME Band 4 1 family domain signature 2

CONSENSUS [HYW]-x(9)-[DENQSTV]-[SA]-x(3)-[FY]-[LIVM]-x(2)-[ACV]-x(2)-[LM]-x(2)-

CONSENSUS [FY]-G-x-[DENQSTHLIVMFYS]

NAME Band 4 1 family domain profile

NAME Clq domam signature

CONSENSUS F-x(5) [ND]-x(4)-[FYWL]-x(6)-F-x(5)-G-x-Y-x-F-x-[FY]

NAME C-terminal cystine knot signature

CONSENSUS C-C-x(13)-C-x(2)-[GN]-x(12)-C-x-C-x(2,4)-C

NAME C-terminal cystine knot profile

NAME CUB domain profile

NAME Death domain profile

NAME EGF- ke domam signature 1 CONSENSUS C-x-C-x(5)-G-x(2)-C

NAME EGF-hke domain signature 2 CONSENSUS C-x-C-x(2)-[GP]-[FYW]-x(4,8)-C

NAME Calcium-binding EGF-hke domam pattern signature

CONSENSUS [DEQN]-x-[DEQN](2)-C-x(3,14)-C-x(3,7)-C-x-[DN]-x(4)-[FY]-x-C

NAME Laminin-type EGF-hke (LE) domain signature

CONSENSUS C-x(l ,2)-C-x(₅)-G-x(2)-C-x(2)-C-x(3,4)-[FYW]-x(3, 15)-C

NAME Coagulanon factors 5/8 type C domain (FA58C) signature 1

CONSENSUS [GAS]-W-x(7,l₅)-[Fj W]-[LrV]-x-[LIVFA]-[GSTDEN]-x(6)-[LIVF]-x(2)-[IV]-x-

CONSENSUS [LIVT]-[QKM]-G

NAME Coagulation factors 5/8 type C domain (FA58C) signature 2 CONSENSUS P-x(8, 10)-[LM]-R-x-[GE]-[LIVP]-x-G-C

NAME Forkhead-associated (FHA) domain profile

NAME Fibrinogen beta and gamma chains C-terminal domain signature CONSENSUS W-W-[LIVMFY ]-x(2)-C-x(2)-[GSA]-x(2)-N-G

NAME Type I fϊbronectin domam CONSENSUS C-x(6,8)-[LFY]-x(5)-[FYW]-x-[RK]-x(8,10)-C-x-C-x(6,9)-C

NAME Type II fibronectin collagen-binding domain

CONSENSUS C-x(2)-P-F-x-[FYWI]-x(7)-C-x(8,10)-W-C-x(4)-[DNSR]-[FYW -x(3,5)-[FYW]-x-

CONSENSUS [FYWI]-C

NAME Hemopexin domain signature

CONSENSUS [LIFAT]-x(3)-W-x(2,3)-[PE]-x(2)-[LIVMFY]-[DENQS]-[STA]-[AV]-[LIVMFY]

NAME Kπngle domain signature CONSENSUS [FY]-C-R-N-P-[DNR]

NAME Kπngle domain profile

NAME LDL-receptor class A (LDLRA) domain signature

CONSENSUS C-[VILMAJ-x(5)-C-[DNH]-x(3)-[DENQHT]-C-x(3,4)-[STADE]-[DEH]-[DEJ-x( 1 ,5)-

CONSENSUS C

NAME LDL-receptor class A (LDLRA) domain profile

NAME C-type lectin domain signature

CONSENSUS C-tLIVMFYATG]-x(5,12)-[WL]-x-[DNSR]-x(2)-C-x(5,6)-[FYWLIVSTA]-[LIVMSTA]-

CONSENSUS C

NAME C-type lectin domain profile

NAME Link domain signature

CONSENSUS C-x(15)-A-x(3,4)-G-x(3)-C-x(2)-G-x(8,9)-P-x(7)-C

NAME Osteonectin domain signature 1

CONSENSUS C-x-[DN]-x(2)-C-x(2)-G-[KRH]-x-C-x(6,7)-P-x C-x-C-x(3,5)-C-P

NAME Osteonectin domam signature 2 CONSENSUS F-P-x-R-[IM]-x-D-W-L-x-[NQ]

NAME Somatomedin B domam signature

CONSENSUS C-x-C-x(3)-C-x(₅)-C-C-x-[DN]-[FY]-x(3)-C

NAME Thyroglobulin type-1 repeat signature

CONSENSUS [FYWHP]-x-P-x-C-x(3,4)-G-x-[FY ]-x(3)-Q-C-x(4,10)-C-[FYW]-C-V-x(3,4)-

CONSENSUS [SG]

NAME P-type 'Trefoil' domain signature

CONSENSUS R-x(2)-C-x-[FYPST]-x(3,4)-[ST]-x(3)-C-x(4)-C-C-[FYWH]

NAME Cellulose-binding domain, bactenal type

CONSENSUS W-N-[STAGR]-[STDN]-[LIVM]-x(2)-[GST]-x-[GST]-x(2)-[LrVMFT]-[GA]

NAME Cellulose-binding domain, fungal type

CONSENSUS C-G-G-x(4,7)-G-x(3)-C-x(5)-C-x(3,5)-[NHG]-x-[FYWM]-x(2)-Q-C

NAME Cnitin recognition or binding domain signature CONSENSUS C-x(4,5)-C-C-S-x(2)-G-x-C-G-x(4)-[FYW]-C

NAME Barwin domain signature 1 CONSENSUS C-G-[KR]-C-L-x-V-x-N

NAME Barwin domain signature 2 CONSENSUS V-[DN]-Y-[EQ]-F-V-[DN]-C

NAME BIR repeat

CONSENSUS [HKEPILVY]-x(2)-R-x(3,7)-[FYW]-x(l 1 , 14)-[STAN]-G-[LMF]-X-[FYHDA]-X(4)-

CONSENSUS [DESL]-X(2,3)-C-X(2)-C-X(6)-[WA]-X(9)-H-X(4)-[PRSD]-X-C-X(2)-[LIVMA]

NAME WAP-type 'four-disulfide core' domam signature CONSENSUS C-x-{C}-[DN]-x(2)-C-x(5)-C-C

NAME Phorbol esters / diacylglycerol binding domain

CONSENSUS H-x-[LIVMFYW]-x(8, 1 l)-C-x(2)-C-x(3)-[LIVMFC]-x(5, 10)-C-x(2)-C-x(4)-[HD]-

CONSENSUS x(2)-C-x(5,9)-C

NAME C2 domain signature

CONSENSUS [ACG]-x(2)-L-x(2,3)-D-x(l,2)-[NGSTLIF]-[GTMR]-x-[STAP]-D-[PA]-[FY] NAME C2 -domain profile

NAME CAP-Gly domain signature

CONSENSUS G-x(8,10)-[FYW]-x-G-[LIVM]-x-[LIVMFY]-x(4)-G-K-[NH]-x-G-[STAR]-x(2)-G-

CONSENSUS x(2)-[LY]-F

NAME Ly-6 / u-PAR domain signature

CONSENSUS [EQR]-C-[LIVMFYAH]-x-C-x(5,8)-C-x(3,8)-[EDNQSTV]-C-{C}-x(5)-C-

CONSENSUS x(12,24)-C

NAME MAM domain signature

CONSENSUS G-x-[LIVMFY](2)-x(3)-[STA]-x(10, 1 l)-[LV]-x(4)-[LIVMF]-x(6,7)-C-[LIVM]-x-

CONSENSUS F-x-[LIVMFY]-x(3)-[GSC]

NAME MAM domain profile

NAME PH domain profile

NAME Phosphotyrosine interaction domain (PID) profile

NAME Src homology 2 (SH2) domain profile

NAME Src homology 3 (SH3) domain profile

NAME VWFC domain signature

CONSENSUS C-x(2,3)-C-x-C-x(6,14)-C-x(3,4)-C-x(2,10)-C-x(9,16)-C-C-x(2,4)-C

NAME W /rsp5/WWP domain signature

CONSENSUS W-x(9, 1 l)-[VFY]-[FYW]-x(6,7)-[GSTNE]-[GSTQCR]-[FYW]-x(2) P

NAME W /rsp5/WWP domain profile

NAME ZP domain signature

CONSENSUS [LIVMFYW]-x(7)-[STAPDNL]-x(3)-[LIVMFVVV]-x-[LIVMFYW]-x-[LrVMFYW]-x(2)-C- CONSENSUS [LIVMFYW]-x-[ST]-[PSL]-x(2,4)-[DENS]-x-[STADNQLF]-x(6)-[LIVM](2)-x(3,4)- CONSENSUS C

NAME S-layer homology domain signature

CONSENSUS [LVFYT]-x-[DA]-x(2,5)-[DNGSATPHY]-[WYFPDA]-x(4)-[LIV]-x(2)-[GTALV]-

CONSENSUS x(4,6)-[LIVFYC]-x(2)-G-x-[PGSTA]-x(2,3)-[MFYA]-x-[PGAV]-x(3,10)-[LIVMA]-

CONSENSUS [STKR]-[RY]-x-[EQ]-x-[STALIVM]

NAME 'Homeobox' domain signature

CONSENSUS [LIVMFYG]-[ASLVR]-x(2)-[LIVMSTACN]-x-[LIVM]-x(4)-[LIV]-[RKNQESTAIY]-

CONSENSUS [LIVFSTNKH]-W-[FYVC]-x-[NDQTAH]-x(5)-[R NAIMW]

NAME 'Homeobox' domain profile

NAME 'Homeobox' antennapedia-type protein signature CONSENSUS [LIVMFE]-[FY]-P-W-M-[KRQTA]

NAME 'Homeobox' engrailed-type protein signature CONSENSUS L-M-A-Q-G-L-Y-N

NAME 'Paired box' domain signature CONSENSUS R-P-C-x( 11 )-C-V-S

NAME 'POU' domain signature 1

CONSENSUS [RKQ]-R-[LIM]-x-[LF]-G-[LIVMFY]-x-Q-x-[DNQ]-V-G

NAME 'POU' domain signature 2

CONSENSUS S-Q-[ST]-[TA]-I-[SC]-R-F-E-x-[LSQ]-x-[LI]-[ST]

NAME Zinc finger, C2H2 type, domam

CONSENSUS C-x(2,4)-C-x(3)-[LIVMFYWC]-x(8)-H-x(3,5)-H

NAME Zinc finger, C3HC4 type (RING finger), signature CONSENSUS C-x-H-x-[LIVMFY]-C-x(2)-C-[LIVMYA]

NAME Nuclear hormones receptors DNA-bindmg region signature CONSENSUS C-x(2)-C-x-[DE]-x(5)-[HN]-[FY]-x(4)-C-x(2)-C-x(2)-F-F-x-R

NAME GATA-type zinc finger domain

CONSENSUS C-x-[DN]-C-x(4,5)-[ST]-x(2)-W-[HR]-[RK]-x(3)-[GN]-x(3,4)-C-N-[AS]-C NAME Poly(ADP-nbose) polymerase zinc finger domain signature CONSENSUS C-[KR]-x-C-x(3)-I-x-K-x(3)-[RG]-x(16,18)-W-[FYH]-H-x(2)-C

NAME Poly(ADP-πbose) polymerase zmc finger domain profile

NAME Fungal Zn(2)-Cys(6) binuclear cluster domain signature

CONSENSUS [GASTPV]-C-x(2)-C-[RKHSTACW]-x(2)-[RKHQ]-x(2)-C-x(5,12)-C-x(2)-C-x(6,8)-

CONSENSUS C

NAME Fungal Zn(2)-Cys(6) bmuclear cluster domain profile

NAME Prokaryotic dksA/traR C4-type zinc finger CONSENSUS C-[DES]-x-C-x(3)-I-x(3)-R-x(4)-P-x(4)-C-x(2)-C

NAME Copper-fist domain signature

CONSENSUS M-[LIVMF](3)-x(3)-K-[MY]-A-C-x(2)-C-I-[KR]-x-H-[KR]-x(3)-C-x-H-x(8)-

CONSENSUS [KR]-x-[KR]-G R-P

NAME Copper fist DNA binding domain profile

NAME Leucine zipper pattern CONSENSUS L-x(6)-L-x(6)-L-x(6)-L

NAME bZIP transcription factors basic domain signature

CONSENSUS [KR]-x( 1 ,3)-[RKSAQ]-N-x(2)-[SAQ](2)-x-[RKTAENQ}-x-R-x-[RK]

NAME Myb DNA-binding domain repeat signature 1 CONSENSUS W-[ST]-x(2)-E-[DE]-x(2)-[LIV]

NAME Myb DNA-binding domam repeat signature 2

CONSENSUS W-x(2)-[LI]-[SAG]-x(4,5)-R-x(8)-[YWl-x(3)-[LrVM]

NAME Myc-type, 'helix-loop-helix' dimerization domain signature

CONSENSUS [DENSTAP]-K [LIVMWAGSN]-{FYWCPHKR}-[LIVT]-[LIV]-x(2)-[STAV]-[LIVMSTAC]-x-

CONSENSUS [VMFYH]-[LIVMTA]-{P}-{P}-[LIVMSR]

NAME p53 tumor antigen signature CONSENSUS M-C-N-S-S-C-M-G-G-M-N-R-R

NAME CBF-A/NF-YB subunit signature

CONSENSUS C-V-S-E-x-I-S-F-[LIVM]-T-[SG]-E-A-[SC]-[DE]-[KRQ]-C

NAME CBF-B/NF-YA subunit signature

CONSENSUS Y-V-N-A-K-Q-Y-x-R-I-L-K-R-R-x-A-R-A-K-L-E

NAME 'Cold-shock' DNA-bindmg domain signature

CONSENSUS [FY]-G-F-I-x(6,7)-[DER]-[LIVM]-F-x-H-x-[STKR]-x-[LIVMFY]

NAME CTF/NF-I signature

CONSENSUS R-K-R-K-Y-F-K-K-H-E-K-R

NAME Ets-domain signature 1

CONSENSUS L-[FYW]-[QEDH]-F-[LI]-[LVQK]-x-[Lη-L

NAME Ets-domain signature 2

CONSENSUS [RKH]-x(2)-M-x-Y-[DENQ]-x-[LIVM]-[STAG]-R-[STAG]-[LI]-R-x-Y

NAME Ets-domain profile

NAME Fork head domam signature 1

CONSENSUS [KR]-P-[PTQ]-[FYLVQH]-S-[FY]-x(2)-[LIVM]-x(3,4)-[AC]-[LIM]

NAME Fork head domain signature 2 CONSENSUS W-[QKR]-[NS]-S-ΓLIV]-R-H

NAME Fork head domain profile

NAME HSF-type DNA-binding domain signature

CONSENSUS L-x(3)-[FY]-K-H-x-N-x-[STAN]-S-F-[LrVM]-R-Q-L-[NH]-x-Y-x-[FYW -[RKH]-K-

CONSENSUS [LIVM]

NAME Tryptophan pentad repeat (IRF family) signature

CONSENSUS W-x-[DNH]-x(5)-[LIVF]-x-[IV]-P-W-x-H-x(9,10)-[DE]-x(2)-[LIVF]-F-[KRQ]-x- CONSENSUS [WR]-A

NAME LIM domain signature

CONSENSUS C-x(2)-C-x(15,21)-[FYWH]-H-x(2)-[CH]-x(2)-C-x(2)-C-x(3)-[LIVMF]

NAME LIM domain profile

NAME NF-kappa-B/Rel/dorsal domain signature CONSENSUS F-R-Y-x-C-E-G

NAME MADS-box domain signature

CONSENSUS R-x-[RK]-x(5)-I-x-[DN]-x(3)-[KR]-x(2)-T-[FY]-x-[RK](3)-x(2)-[LIVM]-x-

CONSENSUS K(2)-A-x-E-[LIVM]-[ST]-x-L-x(4)-[LIVM]-x-[LIVM](3)-x(6)-[LIVMF]-x(2)-

CONSENSUS [FY]

NAME MADS-box domain profile

NAME T-box domain signature 1

CONSENSUS L-W-x(2)-[FC]-x(3,4)-[NT]-E-M-[LIV](2)-T-x(2)-G-[RG]-[KRQ]

NAME T-box domain signature 2

CONSENSUS [LIVMYW]-H-[PADH]-[DEN]-[GS]-x(3)-G-x(2)-W-M-x(3)-[IVA]-x-F

NAME TEA domain signature

CONSENSUS G-R-N-E-L-I-x(2)-Y-I-x(3)-[TC]-x(3)-R-T-[RK](2)-Q-[LIVM]-S-S-H-[LIVM]-

CONSENSUS Q-V

NAME Transcnption factor TFIIB repeat signature

CONSENSUS G-[KR]-x(3)-[STAGN]-x-[LIVMYA]-[GSTA](2)-[CSAV]-[LIVM]-[LIVMFY]-[LIVMA]-

CONSENSUS [GSAHSTAC]

NAME Transcription factor TFIID repeat signature

CONSENSUS Y-x-P-x(2)-[IF]-x(2)-[LIVM](2)-x-[KRH]-x(3)-P-[RKQ]-x(3)-L-[LIVM]-F-x-

CONSENSUS [STN]-G-[KR]-[LIVM]-x(3)-G-[TAGL]-[KR]-x(7)-[AGC]-x(7)-[LIVM]

NAME TFIIS zinc ribbon domain signature

CONSENSUS C-x(2)-C-x(9)-[LIVMQSAR]-[QH]-[STQL]-[RA]-[SACR]-x-[DE]-[DET]-[PGSEA]-

CONSENSUS x(6)-C-x(2,5)-C-x(3)-[FW]

NAME TSC-22 / dip / bun family signature CONSENSUS M-D-L-V-K-x-H-L-x(2)-A-V-R-E-E-V-E

NAME Prokaryotic transcription elongation factors signature 1

CONSENSUS [ST]-x(2)-[GS]-x(3)-[LI]-x(2)-E-L-x(2)-L-x(3,4)-R-x(2)-[IV]-x(3)-[LIV]-

CONSENSUS x(6)-G-D-x(2)-E-N-[GSA]-x-Y

NAME Prokaryotic transcnption elongation factors signature 2

CONSENSUS S-x(2)-S-P-[LIVM]-[AG]-x-[SAG]-[LIVM]-[LIVMY]-x(4)-[DG]-[DE]

NAME DEAD-box subfamily ATP-dependent helicases signature CONSENSUS tLrVMF](2)-D-E-A-D-[RKEN]-x-[LIVMFYGSTN]

NAME DEAH-box subfamily ATP-dependent helicases signature CONSENSUS [GSAH]-x-[LIVMF](3)-D-E-[ALIV]-H-[NECR]

NAME Eukaryotic putative RNA-binding region RNP-1 signature CONSENSUS [RK]-G-{EDRKHPCG}-[AGSCI]-[FY]-[LIVA]-x-[FYLM]

NAME Fibπllaπn signature

CONSENSUS [GST]-[LIVMAP]-V-Y-A-[TV]-E-[FY]-[SA]-x-R-x(2)-R-[DE]

NAME MCM family signature

CONSENSUS G-[IVT]-[LVAC](2)-[IVT]-D-[DE]-[FL]-[DNST]

NAME MCM family domain

NAME XPA protein signature 1

CONSENSUS C-x-[DE]-C-x(3)-[LIVMF]-x( 1 ,2)-D-x(2)-L-x(3)-F-x(4)-C-x(2)-C

NAME XPA protein signature 2

CONSENSUS [LIVM](2)-T-[KR]-T-E-x-K-x-[DE]-Y-[LIVMF](2)-x-D-x-[DE]

NAME XPG protein signature 1

CONSENSUS [VI]-[KRE]-P-x-[FYIL]-V-F-D-G-x(2)-[PIL]-x-[LVC]-K NAME. XPG protein signature 2

CONSENSUS: [GS]-[LIVM]-[PER]-[FYS]-[LIVM]-x-A-P-x-E-A-[DE]-[PAS]-[QS]-[CLM]

NAME Bacterial regulatory proteins, araC family signature.

CONSENSUS: [KRQ]-[LIVMA]-x(2)-[GSTALIV]-{FYWPGDN}-x(2)-[LIVMSA]-x(4,9)-[LIVMF]-

CONSENSUS: x(2)-[LIVMSTA]-[GSTACIL]-x(3)-[GANQRF]-[LIVMFY]-x(4,5)-[LFY]-x(3)-

CONSENSUS- [FYIVA]-{FYWHCM}-x(3)-[GSADENQKR]-x-[NSTAPKL]-[PARL].

NAME: Bacteπal regulatory proteins, araC family DNA-bindmg domain profile.

NAME- Bacterial regulatory proteins, arsR family signature CONSENSUS: C-x(2)-D-[LIVM]-x(6)-[ST]-x(4)-S-[HYR]-[HQ]

NAME: Bacterial regulatory proteins, asnC family signature.

CONSENSUS- [GSTAP]-x(2)-[DNEA]-[LIVM]-[GSA]-x(2)-[LIVMFY]-[GN]-[LIVMST]-[ST]-x(6)-R-

CONSENSUS: [LVT]-x(2)-[LIVM]-x(3)-G

NAME: Bacterial regulatory proteins, crp family signature.

CONSENSUS: [LIVM]-[STAG]-[RHNW]-x(2)-[LIM]-[GA]-x-[LIVMFYA]-[LIVSC]-[GA]-x-[STACN]-

CONSENSUS: x(2)-[MST]-x-[GSTN]-R-x-[LIVMF]-x(2)-[LIVMF] .

NAME: Bacterial regulatory proteins, deoR family signature

CONSENSUS- R-x(3)-[LIVM]-x(3)-[LIVM]-x(16,17)-[STA]-x(2)-T-[LIVMA]-[RH]-[KRNA]-D-

CONSENSUS: [LIVMF].

NAME: Bacterial regulatory proteins, gntR family signature

CONSENSUS- [LIVAPKR]-[PILV]-x-[EQTIVMR]-x(2)-[LIVM]-x(3)-[LIVMFYK]-x-[LIVFT]-

CONSENSUS. [DNGSTK]-[RGTLV]-x-[STAIVP]-rLIVA]-x(2)-[STAGV]-[LIVMFYH]-x(2)-[LMA].

NAME Bacterial regulatory proteins, ICIR family signature

CONSENSUS: [GA]-x(3)-[DS]-x(2)-E-x(6)-[CSA]-[LIVM]-[GSA]-x(2)-[LIVM]-[FYH]-[DN].

NAME: Bacterial regulatory proteins, lad family signature

CONSENSUS [LIVM]-x-[DE]-[LIVM]-A-x(2)-[STAGV]-x-V-[GSTP]-x(2)-[STAG]-[LIVMA}-x(2)-

CONSENSUS [LIVMFYANHLIVMC]

NAME Bactenal regulatory proteins, luxR family signature.

CONSENSUS: [GDC]-x(2)-[NSTAVY]-x(2)-[IV]-[GSTA]-x(2)-[LIVMFYWCT]-x-[LIVMFYWCR]-x(3)-

CONSENSUS: [NST]-[LIVM]-x(5)-[NRHSA]-[LIVMSTA]-x(2)-[KR]

NAME Bacteπal regulatory proteins, lysR family signature.

CONSENSUS [NQKRHSTAG]-[LIVMFYTA]-x(2)-[STAGLV]-[STAG]-x(4)-[LIVMYCTQR]-[PSTANLVER]-

CONSENSUS x-[PSTAGQV]-[PSTAGNVMF]-[LIVMFA]-[STAGH]-x(2)-[LIVMF]-x(2)-[LIVMFW]-

CONSENSUS: [RKEAV]-x(2)-[LIVMFYNTAE]-x(3)-[LIMVT] .

NAME. Bactenal regulatory proteins, marR family signature.

CONSENSUS: [STNA]-[LIA]-x-[RNGS]-x(4)-[LM]-[EIV]-x(2)-[GES]-[LFYW]-[LIVC]-x(7)-

CONSENSUS: [DN]-[RKQG]-[RK]-x(6)-T-x(2)-[GA]

NAME: Bactenal regulatory proteins, merR family signature.

CONSENSUS. [GSA]-x-[LIVMFA]-[ASM]-x(2)-[STACLIV]-[GSDENQR]-[LIVC]-[STANHK]-x(3)-

CONSENSUS [LIVM]-[RHF]-x-[YW]-[DEQ]-x(2,3)-[GHDNQ]-[LIVMF](2).

NAME: Bactenal regulatory proteins, tetR family signature.

CONSENSUS G-[LIVMFYS]-x(2,3)-[TS]-[LIVMT]-x(2)-[LIVM]-x(5)-[LIVQS]-[STAGENQH]-x-

CONSENSUS- [GPAR]-x-[LIVMF]-[FYST]-x-[HFY]-[FV]-x-[DNST]-K-x(2)-[LIVM].

NAME' Transcnptional antiterminators bglG family signature. CONSENSUS' [ST]-x-H-x(2)-[FA](2)-[LIVM]-[EQK]-R-x(2)-[QNK].

NAME- Sιgma-54 factors family signature 1.

CONSENSUS: P-[LIVM]-x-tLIVM]-x(2)-[LIVM]-A-x(2)-[LIVMF]-x(2)-[HS]-x-S-T-[LIVM]-S-R.

NAME. Sιgma-54 factors family signature 2. CONSENSUS: R-R-T-[IV]-[AT]-K-Y-R.

NAME: Sιgma-54 factors family profile.

NAME: Sιgma-70 factors family signature 1.

CONSENSUS: [DE]-[LIVMF](2)-[HEQS]-x-G-x-[LIVMFA]-G-L-[LIVMFYE]-x-[GSAM]-[LIVMAP].

NAME: Sιgma-70 factors family signature 2

CONSENSUS: [STN]-x(2)-[DEQ]-[LIVM]-[GAS]-x(4)-[LIVMF]-[PSTG]-x(3)-rLIVMA]-x-[NQR]- CONSENSUS [LIVMA]-[EQH]-x(3)-[LIVMFW]-x(2)-[LIVM]

NAME Sιgma-70 factors ECF subfamily signature

CONSENSUS [STAIV]-[PQDEL]-[DE]-[LIV]-[LIVTA]-Q-x-[STAV]-[LIVMFYC]-[LIVMAK]-x-

CONSENSUS [GSTAIV]-[LIMFYWQ]-x(12,14)-[STAP]-[FYW]-[LIF]-x(2)-[IV]

NAME Sιgma-54 interaction domain ATP-binding region A signature CONSENSUS [LIVMFY](3)-x-G-[DEQ]-[STE]-G-[STAV]-G-K-x(2)-[LrVMFY]

NAME Sιgma-54 interaction domain ATP-bmding region B signature

CONSENSUS [GS]-x-[LIVMF]-x(2)-A-[DNEQASH]-[GNEK]-G-[STIM]-[LIVMFY](3)-[DE]-[E ]-

CONSENSUS [LIVM]

NAME Sιgma-54 interaction domain C-terminal part signature CONSENSUS [FY ]-P-[GS]-N-[LIVM]-R-[EQ]-L-x-[NHAT]

NAME Sιgma-54 interaction domain profile

NAME Single-strand binding protein family signature 1

CONSENSUS [LIVMF]-[NST]-[KRT]-[LIVM]-x-[LIVMF](2)-G-[NHRK]-[LrVM]-[GST]-x-[DET]

NAME Single-strand binding protein family signature 2

CONSENSUS T-x-W-[HY]-[RNS]-[LIVM]-x-[LIVMF]-[FY]-[NGKR]

NAME Bacterial histone- ke DNA-binding proteins signature

CONSENSUS [GSK]-F-x(2) [LIVMF]-x(4)-[RKEQA]-x(2)-[RST]-x-[GA]-x-[KN]-P-x-T

NAME Dps protein family signature 1

CONSENSUS H-[FW]-x-[LIVM]-x-G-x(5)-[LV]-H-x(3)-[DE]

NAME Dps protein family signature 2

CONSENSUS [LIVMFY]-[DH]-x-[LIVM]-[GA]-E-R-x(3)-[LIF)-[GDN]-x(2)-[PA]

NAME DNA repair protein radC family signature CONSENSUS H-N-H-P-S-G

NAME recA signature

CONSENSUS A-L-[KR]-[IF]-[FY]-[STA]-[STAD]-[LIVMQ]-R

NAME RecF protein signature 1

CONSENSUS P-[ED]-x(3)-[LIVM](2)-x-G-[GSAD]-P-x(2)-R-R-x-[FY]-[LIVM]-D

NAME RecF protein signature 2

CONSENSUS [LIVMFY](2)-x-D-x(2,3)-[SA]-[EH]-L-D-x(2)-[KRH]-x(3)-L

NAME RecR protein signature

CONSENSUS C-x(2)-C-x(3)-[ST]-x(4)-C-x-I-C-x(4)-R

NAME Histone H2A signature CONSENSUS [AC]-G-L-x-F-P-V

NAME Histone H2B signature

CONSENSUS [KR]-E-[LIVM]-[EQ]-T-x(2)-[KR]-x-[LIVM](2)-x-[PAG]-[DE]-L-x-[KR]-H-A-

CONSENSUS [LIVM]-[STA]-E-G

NAME Histone H3 signature 1 CONSENSUS K-A-P-R-K-Q-L

NAME Histone H3 signature 2

CONSENSUS P-F-x-[RA]-L-[VA]-[KRQJ-[DEG]-[IV]

NAME Histone H4 signature CONSENSUS G-A-K-R-H

NAME HMG1/2 signature

CONSENSUS [FI]-S-[KR]-K-C-S-[EK]-R-W-K-T-M

NAME HMG-I and HMG-Y DNA-binding domain (A + T-hook) CONSENSUS [AT]-x(l,2)-[RK](2)-[GP]-R-G-R-P-[RK]-x

NAME HMG14 and HMG17 signature CONSENSUS R-R-S-A-R-L-S-A-[RK]-P

NAME Bromodomam signature CONSENSUS [STANVF]-x(2)-F-x(4)-[DNS]-x(5,7)-[DENQTF]-Y-[HFY]-x(2)-[LIVMFY]-x(3)-

CONSENSUS [LIVM]-x(4)-[LIVM]-x(6,8)-Y-x(12,13)-[LIVM]-x(2)-N-[SACF]-x(2)-[FY]

NAME Bromodomain profile

NAME Chromo domain signature

CONSENSUS tFYL]-x-[LIVMC]-[KR]- -x-[GDNR]-[FYWLE]-x(5,6)-[ST]-W-[ES]-[PSTDN]-x(3)-

CONSENSUS [LIVMC]

NAME Chromo and chromo shadow domain profile

NAME Regulator of chromosome condensation (RCCl) signature 1 CONSENSUS G-x-N-D-x(2)-[AV]-L-G-R-x-T

NAME Regulator of chromosome condensation (RCCl) signature 2

CONSENSUS [LIVMFA]-[STAGC}(2)-G-x(2)-H-[STAGLI]-[LIVMFA]-x-[LIVM]

NAME Protamine PI signature

CONSENSUS [AV]-R-[NFY]-R-x(2,3)-[ST]-x-S-x-S

NAME Nuclear transition protein 1 signature CONSENSUS S-K-R-K-Y-R-K

NAME Nuclear transition protein 2 signature 1 CONSENSUS H-x(3)-H-S-[NS]-S-x-P-Q-S

NAME Nuclear transition protein 2 signature 2 CONSENSUS K-x-R-K-x(2)-E-G-K-x(2)-K-[KR]-K

NAME Ribosomal protein LI signature

CONSENSUS [IM]-x(2)-[LIVA]-x(2,3)-[LIVM}-G-x(2)-[LMS]-[GSNH]-tPTKR]-[KRAV]-G-x-

CONSENSUS [LMF]-P-[DENSTK]

NAME Ribosomal protein L2 signature

CONSENSUS P-x(2)-R-G-[STAIV](2)-x-N-[APK]-x [DE]

NAME Ribosomal protein L3 signature

CONSENSUS [FL]-x(6)-[DN]-x(2)-[AGS]-x-[ST]-x-G-[KRH]-G-x(2)-G-x(3)-R

NAME Ribosomal protein L5 signature

CONSENSUS [LrVM]-x(2)-[LIVM]-[STAC]-[GE]-[QV]-x(2)-[LIVMA]-x-[STC]-x-[STAG]-[KR]-

CONSENSUS x-[STA]

NAME Ribosomal protein L6 signature 1

CONSENSUS [PS]-[DENSl-x-Y-K-[GA]-K-G-[LIVM]

NAME Ribosomal protein L6 signature 2

CONSENSUS Q-x(3)-[LIVM]-x(2)-[KR]-x(2)-R-x-F-x-D-G-[LrVM]-Y-[LIVM]-x(2)-[KR]

NAME Ribosomal protein L9 signature

CONSENSUS G-x(2)-[GN]-x(4)-V-x(2)-G-[FY]-x(2)-N-[FY]-L-x(5)-[GA]-x(3)-[STN]

NAME Ribosomal protein L10 signature

CONSENSUS [DEH]-x(2)-[GS]-[LIVMF]-[STN]-[VA]-x-[DEQK]-[LIVMA]-x(2)-[LIM]-R

NAME Ribosomal protein LI 1 signature

CONSENSUS [RKN]-x-[LIVM]-x-G-[ST]-x(2)-[SNQ]-[LIVM]-G-x(2)-[LIVM]-x(0,l)-[DENG]

NAME Ribosomal protein L13 signature

CONSENSUS [LIVM]-[KRV]-[GK]-M-[LIV]-[PS]-x(4,5)-[GS]-[NQEKRA]-x(5)-[LIVM]-x-[AIV]-

CONSENSUS [LFY]-x-[GDN]

NAME Ribosomal protein L14 signature

CONSENSUS [GA]-[LIV](3)-x(9,10)-[DNS]-G-x(4)-[FY]-x(2)-[NT]-x(2)-V-[LIV]

NAME Ribosomal protein LI 5 signature

CONSENSUS K-[LIVM](2)-[GAL]-x-[GT]-x-[LIVMA]-x(2,5)-[LIVM]-x-[LIVMF]-x(3,4)-

CONSENSUS [LIVMFC]-[ST]-x(2)-A-x(3)-[LIVM]-x(3)-G

NAME Ribosomal protein L16 signature 1

CONSENSUS [KR]-R-x-[GSAC]-[KQVA]-[LIVM]-W-[LIVM]-[KR]-[LrVM]-[LFY]-[AP]

NAME Ribosomal protein L16 signature 2 CONSENSUS R-M-G-x-[GR]-K-G-x(4)-[FWKR] NAME Ribosomal protein L17 signature

CONSENSUS I-x-[ST]-[GT]-x(2)-[KR]-x-K-x(6)-[DE]-x-[LIMV]-[LIVMT]-T-x-[STAG]-[KR]

NAME Ribosomal protein L19 signature

CONSENSUS [RT]-[ RSVY]-[GSA]-x-V-[RS]-[KR]-[SA]-K-L-Y-Y-L-R

NAME Ribosomal protein L20 signature

CONSENSUS K-x(3)-[KRC]-x-[LIVM]-W-[IV]-[STNALV]-R-[LIVM]-N-x(3)-[RKH]

NAME Ribosomal protein L21 signature

CONSENSUS [rVT]-x(3)-[KR]-x(3)-[KRQ]-K-x(6)-G-[HF]-R-[RQ]-x(2)-T

NAME Ribosomal protein L22 signature

CONSENSUS [RKQN]-x(4)-[RH]-[GAS]-x-G-[KRQS]-x(9)-[HDN]-[LIVM]-x-[LIVMS]-x-[LIVM]

NAME Ribosomal protein L23 signature

CONSENSUS [RK](2)-[AM]-[IVFYT]-[IV]-[RKT]-L-[STANQK]-x(7)-[LIVMFT]

NAME Ribosomal protein L24 signature

CONSENSUS [GDEN]-D-X-V-X-[IV]-[LIVMA]-X-G-X(2) [KA]-[GN]-X(2,3)-[GA]-X-[IV]

NAME Ribosomal protein L27 signature CONSENSUS G-x-[LIVM](2)-x-R-Q-R-G-x(5)-G Ribosomal protein L29 signature

CONSENSUS [KNQS]-[PSTL]-X(2)-[LIMFA]-[KRGSAN]-X-[LIVYSTA]-[KR]-[KRH]-[DESTANRL]- CONSENSUS [LΓV]-A-[KRCQVT]-[LIVMA]

NAME Ribosomal protein L30 signature

CONSENSUS [rVT]-[LIVM]-x(2)-[LF]-x-[LI]-x-[KRHQEG]-x(2)-[STNQH]-x [IVT]-

CONSENSUS x(10)-[LMS]-[LIV]-x(2)-[LrVA}-x(2)-[LMFY]-[IVT]

NAME Ribosomal protein L31 signature

CONSENSUS H-P-F-[FY]-[TI]-x(9)-G-R-[AV]-x-[KR]

NAME Ribosomal protein L33 signature

CONSENSUS Y-x-[ST]-x-[KR]-[NS]-x(4) [PAT]-x(l ,2)-[LIVM]-[EA]-x(2)-K-[FY]-[CSD]

NAME Ribosomal protein L34 signature

CONSENSUS K-[RG]-T [FYWL]-[EQS]-x(5)-[KRHS]-x(4,5)-G-F-x(2)-R

NAME Ribosomal protein L35 signature

CONSENSUS [LIVM]-K [TV]-x(2)-[GSA]-[SAIL]-x-K-R-[LIVMFY]-[KRL]

NAME Ribosomal protein L36 signature

CONSENSUS C-x(2)-C-x(2)-[LIVM]-x-R-x(3)-[LrVMN]-x-[LIVM]-x-C-x(3,4)-[KR]-H-x-Q-x-Q

NAME Ribosomal protein Lie signature

CONSENSUS N-x(3)-[KR]-x(2)-A-[LIVT]-x-S-A-[LIV]-x A-[ST]-[SGA]-x(7)-[RKl-G-H

NAME Ribosomal protein L6e signature

CONSENSUS N-x(2)-P-L-R-R-x(4)-[FY]-V-I-A-T-S-x-K

NAME Ribosomal protein L7Ae signature

CONSENSUS [CA]-x(4)-[IV]-P-[FY]-x(2)-[LIVM]-x-[GSQ]-[KRQ]-x(2)-L-G

NAME Ribosomal protein LlOe signature

CONSENSUS R-x-A-[FYW]-G-K-[PA]-x-G-x(2)-A-R-V

NAME Ribosomal protein L13e signature

CONSENSUS [KR]-Y-x(2)-K-[LIVM]-R-[STA]-G-[KR]-G-F-[ST]-L-x-E

NAME Ribosomal protein L15e signature

CONSENSUS tDE]-[KR]-A-R-x-L-G-[FY]-x-[SAP]-x(2)-G-[LIVMFY](4)-R-x-R-V-x-R-G

NAME Ribosomal protein L18e signature

CONSENSUS [KRE]-x-L-x(2)-[PS]-[KR]-x(2)-[RH]-[PSA]-x-[LIVM]-[NS]-[LrVM]-x-[RK]-

CONSENSUS [LIVM]

NAME Ribosomal protein L19e signature

CONSENSUS R-x-[KR]-x(5)-[KR]-x(3)-[KRH]-x(2)-G-x-G-x-R-x-G-x(3)-A-R-x(3)-[KQ]-

CONSENSUS x(2)-W-x(7)-R-x(2)-L-x(3)-R NAME Ribosomal protein L21e signature

CONSENSUS G-[DE]-x-V-x(10)-[GV]-x(2)-[FYH]-x(2)-[FY]-x-G-x-T-G

NAME Ribosomal protein L24e signature

CONSENSUS [FY]-x-[GS]-x(2)-[IV]-x-P-G-x-G-x(2)-[FYV]-x-[KRHE]-x-D

NAME Ribosomal protein L27e signature CONSENSUS G-K-N-x-W-F-F-x-K-L-R-F >

NAME Ribosomal protein L30e signature 1

CONSENSUS [STA]-x(5)-G-x-[QKR]-x(2)-[LIVM]-[KQT]-x(2)-[KR]-x-G-x(2)-K-x-[LIVM](3)

NAME Ribosomal protein L30e signature 2

CONSENSUS [DE]-L-G-[STA]-x(2)-G-[KR]-x(6)-[LIVM]-x-[LIVM]-x-[DEN]-x-G

NAME Ribosomal protein L31e signature

CONSENSUS V-[KR]-[LIVM]-x(3)-[LIVM]-N-x-[AK]-x-W-x-[KR]-G

NAME Ribosomal protein L32e signature

CONSENSUS F-x-R-x(4) [KR]-x(2)-[KR]-[LIVM]-x(3)-W-R-[KR]-x(2)-G

NAME Ribosomal protein L34e signature CONSENSUS Y-x-[ST]-x-S-[NY]-x(5)-[KR]-T-P-G

NAME Ribosomal protein L35Ae signature

CONSENSUS G-K-[LIVM]-x-R-x-H-G-x(2)-G-x-V-x-A-x-F-x(3)-[LI]-P Ribosomal protein L36e signature

CONSENSUS P-Y-E-[KR]-R-x-[LIVM]-[DE]-[LIVM](2)-[KR]

NAME Ribosomal protein L37e signature

CONSENSUS G-T-x-[SA]-x-G-x-[KR]-x(3)-[ST]-x(0,l)-H-x(2)-C-x-R-C-G

NAME Ribosomal protein L39e signature

CONSENSUS [KRA]-T-x(3)-[LIVM]-[KRQF]-x-[NHS]-x(3)-R-[NHY]-W-R-R

NAME Ribosomal protein L44e signature CONSENSUS K-x-[TV]-K-K-x(2)-L-[KR]-x(2)-C

NAME Ribosomal protein S2 signature 1

CONSENSUS [LIVMFA]-x(2)-[LIVMFYC](2)-x-[STAC]-[GSTANQEKR]-[STALV]-[HY]-[LrVMF]-G

NAME Ribosomal protein S2 signature 2

CONSENSUS P-X(2)-[LIVMF](2)-[LΓVMS]-X-[GDN]-X(3)-[DENL]-X(3)-[LIVM]-X-E-X(4)-

CONSENSUS [GNQKRH]-[LIVM]-[AP]

NAME Ribosomal protein S3 signature

CONSENSUS [GSTA]-[KR]-x(6)-G-x-[LIVMT]-x(2)-[NQSCH]-x(l,3)-[LIVFCA]-x(3)-[LIVl-

CONSENSUS [DENQ]-x(7)-[LMT]-x(2)-G-x(2)-G

NAME Ribosomal protein S4 signawre

CONSENSUS [LIVM]-[DE]-x-R-L-x(3)-[LIVMC]-[VMFYHQ]-[KRT]-x(3)-[STAGCF]-x-[ST]-x(3)-

CONSENSUS [SAI]-[KR]-x-[LIVMF](2)

NAME Ribosomal protein S5 signature

CONSENSUS G-[KRQ]-x(3)-[FY]-x-[ACV]-x(2)-[LIVMA]-[LIVM]-[AG]-[DN]-x(2)-G-x-

CONSENSUS [LrVM]-G-x-[SAG]-x(5,6)-[DEQ]-[LIVM]-x(2)-A-[LIVMF]

NAME Ribosomal protein S6 signature

CONSENSUS G-x-[KRC]-[DENQRH]-L-[SA]-Y-x-I-[KRNSA]

NAME Ribosomal protein S7 signature

CONSENSUS [DENSK]-x-[LIVMET]-x(3)-[LrVMFT](2)-x(6)-G-K-tKR]-x(5)-[LIVMF]-[LIVMFC]-

CONSENSUS x(2)-[STA]

NAME Ribosomal protein S8 signature

CONSENSUS [GE]-x(2)-[LIV](2)-[STY]-T-x(2)-G-[LIVM](2)-x(4)-[AG]-[KRHAYI]

NAME Ribosomal protein S9 signature

CONSENSUS G-G-G-x(2)-[GSA]-Q-x(2)-[SA]-x(3)-[GSA]-x-[GSTAV]-[KR]-[GSAL]-[LIF]

NAME Ribosomal protein S10 signature

CONSENSUS [AV]-x(3)-[GDNSR]-[LIVMSTA]-x(3)-G-P-[LIVM]-x-[LIVM]-P-T NAME Ribosomal protein Sl l signature

CONSENSUS [LP MF]-x-[GSTAC]-[LIVMF]-x(2)-[GSTAL]-x(0, l)-[GSN]-[LIVMF]-x-[LIVM]-

CONSENSUS x(4)-[DEN]-x-T-P-x-[PA]-[STCH]-[DN]

NAME Ribosomal protein S12 signature CONSENSUS [RK]-x-P-N-S-[AR]-x-R

NAME Ribosomal protein S13 signature

CONSENSUS [KRQS]-G-x-R-H-x(2)-[GSNH}-x(2)-[LIVMC]-R-G-Q

NAME Ribosomal protein S14 signature

CONSENSUS [RP]-x(0,l)-C-x(l l,12)-[LIVMF]-x-[LIVMF]-[SC]-[RG]-x(3)-[RN]

NAME Ribosomal protein S15 signature

CONSENSUS [LrVM]-x(2)-H-[LIVMFY]-x(5)-D-x(2)-[SAGN]-x(3)-[LF]-x(9)-[LIVM]-x(2)-

CONSENSUS [FY]

NAME Ribosomal protein S16 signature

CONSENSUS [LIVMT]-x-[LIVM]-[KR]-L-[STAK]-R-x-G-[AKR]

NAME Ribosomal protein S17 signature

CONSENSUS G-D-x-[LIV]-x-[LIVA]-x-[QEK]-x-[RK]-P-[LIV]-S

NAME Ribosomal protein S18 signature

CONSENSUS [IV]-[DY]-Y-x(2) [LIVMT]-x(2)-[LIVM]-x(2) [FYT]-[LIVM]-[ST]-[DERP]-x-

CONSENSUS [GY]-K-[LIVM]-x(3)-R-[LIVMAS]

NAME Ribosomal protein S19 signature

CONSENSUS [STDNQ]-G-[KRQM]-x(6)-[LIVM]-x(4)-[LIVM]-[GSD]-x(2)-[LF]-[GAS]-[DE]-F-

CONSENSUS x(2)-[ST]

NAME Ribosomal protein S21 signature

CONSENSUS [DE]-x-A-[LY]-[KR]-R-F-K-[KR]-x(3)-[KR]

NAME Ribosomal protein S3Ae signature

CONSENSUS [LIV]-x-[GH]-R-[rV]-x-E-x-[SC]-L-x-D-L

NAME Ribosomal protein S4e signature

CONSENSUS H-x-K-R-[LIVM]-[SAN]-x-P-x(2)-W-x-[LIVM]-x-[KR]

NAME Ribosomal protein S6e signature

CONSENSUS [LIVM] [STAMR]-G-G-x-D-x(2)-G-x-P-M

NAME Ribosomal protein S7e signature

CONSENSUS [KR]-L-x-R-E-L-E-K-K-F-[SAP]-x-[KR]-H

NAME Ribosomal protein S8e signature

CONSENSUS R-x(2)-T-G-[GA]-x(5)-[HR]-K [KR]-x-K-x-E-[LM]-G

NAME Ribosomal protein S12e signature

CONSENSUS A-L-[KRQP]-x-V-L-x(2)-[SA]-x(3)-[DN]-G-L

NAME Ribosomal protein S17e signature

CONSENSUS A-x-I-x-[ST]-K-x-L-R-N-[KR]-I-A-G-[FY]-x-T-H

NAME Ribosomal protein S19e signature

CONSENSUS P-x(6)-[SAN]-x(2)-[LIVMA]-x-R-x-[ALIV]-[LV]-Q-x-L-[EQ]

NAME Ribosomal protein S21e signature CONSENSUS L-Y-V-P-R-K-C-S-[SA]

NAME Ribosomal protein S24e signature

CONSENSUS [FA]-G-x(2)-[KR]-[STA]-x-G-[FY]-[GA]-x-[LIVM]-Y-[DN]-[SN]

NAME Ribosomal protein S26e signature CONSENSUS [YH]-C-V-S-C-A-I-H

NAME Ribosomal protein S27e signature

CONSENSUS [QK]-C-x(2)-C-x(6)-F-[GS]-x-[PSA]-x(5)-C-x(2)-C-[GS]-x(2)-L-x(2)-P-x-G

NAME Ribosomal protein S28e signature CONSENSUS E-[ST]-E-R-E-A-R-x-L

NAME DNA mismatch repair proteins mutL / hexB / PMS1 signature CONSENSUS G-F-R-G-E-A-L

NAME DNA mismatch repair proteins mutS family signature

CONSENSUS [ST]-[LIVM]-x-[LIVM]-x-D-E-[LIVMY]-[GC]-[RKH]-G-[GST]-x(4)-G

NAME muiT domain signature

CONSENSUS G-x(5)-E-x(4)-[STAGCJ-[LIVMAC]-x-R-E-[LIVMFT]-x-E-E

NAME DnaA protein signature

CONSENSUS I-[GA]-x(2)-[LIVMF]-[SGDNK]-x(0,l)-[KR]-x-H-[STP]-[STV]-[LIVM](2)-x-

CONSENSUS [SA]-x(2)-[KRE]-[LIVM]

NAME Small, acid-soluble spore proteins, alpha/beta type, signature 1 CONSENSUS K-x-E-[LIV]-A-x-[DE]-[LIVMF]-G-[LIVMFl

NAME Small, acid-soluble spore proteins, alpha/beta type, signature 2 CONSENSUS [KR]-[SAQ]-x-G-x-V-G-G-x-[LIVM]-x-[KR](2)-[LIVM](2)

NAME Zinc-containing alcohol dehydrogenases signature CONSENSUS G-H-E-x(2)-G-x(5)-[GA]-x(2)-[IVSAC]

NAME Qumone oxidoreductase / zeta-crystal n signature

CONSENSUS [GSD]-[DEQH]-x(2)-L-x(3)-[SA](2)-G-G-x-G-x(4)-Q-x(2)-[KR]

NAME Iron-containing alcohol dehydrogenases signature 1

CONSENSUS [STALIV]-[LIVF]-x-[DE]-x(6,7)-P-x(4)-[ALrV]-x-[GST]-x(2)-D-[TAIVM]-

CONSENSUS [LIVMF]-x(4)-E

NAME Iron-containing alcohol dehydrogenases signature 2

CONSENSUS [GSW]-x-[LIVTSACD]-[GH]-x(2)-[GSAE]-[GSHYQ]-x-[LrVTPHGAST]-[GAS]-x(3)-

CONSENSUS [LIVMT]-x-[HNS]-[GA]-x-[GTAC]

NAME Short-chain dehydrogenases/reductases family signature

CONSENSUS [LIVSPADNK]-x(12)-Y-[PSTAGNCV]-[STAGNQCIVM]-[STAGC] K-{PC}-[SAGFR]-

CONSENSUS [LIVMSTAGD]-x(2)-[LIVMFYW]-x(3)-[LIVMFYWGAPTHQ]-[GSACQRHM]

NAME Aldo/keto reductase family signature 1

CONSENSUS G-[FY]-R-[HSAL]-[LIVMF]-D-[STAGC]-[AS]-x(5)-E-x(2)-[LIVM]-G

NAME Aldo/keto reductase family signature 2

CONSENSUS [LIVMFY]-x(9)-[KREQ]-x-[LIVM]-G-[LIVM]-[SC]-N-[FY]

NAME Aldo/keto reductase family putative active site signature

CONSENSUS [LIVM]-[PAIV]-[KR]-[ST]-x(4)-R-x(2)-[GSTAEQK]-[NSL]-x(2)-[LIVMFA]

NAME Homoseπne dehydrogenase signature

CONSENSUS A-x(3)-G-[LrVMFY]-[STAG]-x(2,3)-[DNS] P-x(2)-D-[LIVM]-x-G-x-D-x(3)-K

NAME NAD-dependent glycerol-3-phosphate dehydrogenase signature

CONSENSUS G-[AT]-[LIVM]-K-[DN]-[LIVM](2)-A-x-[GA]-x-G-[LIVMF]-x-[DE]-G-[LIVM]-x-

CONSENSUS [LIVMFYW]-G-x-N

NAME FAD-dependent glycerol-3-phosphate dehydrogenase signature 1 CONSENSUS [IV]-G-G-G-x(2)-G-[STACV]-G-x-A-x-D-x(3)-R-G

NAME FAD-dependent glycerol-3-phosphate dehydrogenase signature 2 CONSENSUS G-G-K-x(2)-[GSTE]-Y-R-x(2)-A

NAME Mannitol dehydrogenases signature

CONSENSUS [LIVMY]-x-[FS]-x(2)-[STAGCV]-x-V-D-R-[IV]-x-[PS]

NAME Histidinol dehydrogenase signature

CONSENSUS I-D-x(2)-A-G-P-[ST]-E-[LIVS]-[LIVMA](3)-[AC]-x(3)-A-x(4)-[LIVM]-[AV]-

CONSENSUS [SACL]-[DE]-[LIVMFC]-[LIVM]-[SA]-x(2)-E-H

NAME L-lactate dehydrogenase active site CONSENSUS [LIVMA]-G-[EQ]-H-G-[DN]-[ST]

NAME D-isomer specific 2-hydroxyacid dehydrogenases NAD-binding signature

CONSENSUS [LIVMA]-[AG]-[IVT]-[LIVMFY]-[AG]-x-G-[NHKRQGSAC]-[LrV]-G-x(13,14)-

CONSENSUS [LIVfMT]-x(2)-[FYwCTH]-[DNSTK]

NAME D-isomer specific 2-hydroxyacid dehydrogenases signature 2

CONSENSUS [LIVMFYWA]-[LIVFYWC]-x(2)-[SAC]-[DNQHR]-[IVFA]-[LIVF]-x-[LIVF]-[HNri-x- CONSENSUS P-x(4)-[STN]-x(2)-[LIVMF]-x-[GSDN]

NAME D-isomer specific 2-hydroxyacid dehydrogenases signature 3

CONSENSUS [LMFATC]-[KPQ]-x-[GSTDN]-x-[LIVMFYWR]-[LIVMFY ](2)-N-x-[STAGC]-R-[GP]-x-

CONSENSUS [LIVH]-[LIVMC]-[DNV]

NAME 3-hydroxyιsobutyrate dehydrogenase signature

CONSENSUS [LIVMFY](2)-G-L-G-x-[MQ]-G-x-[PGS]-[MA]-[SA]

NAME Hydroxymethylglutaryl-coenzyme A reductases signature 1 CONSENSUS [RKH]-x(6)-D-x-M-G-x-N-x-[LIVMA]

NAME Hydroxymethylglutaryl-coenzyme A reductases signature 2 CONSENSUS [LIVM]-G-x-[LIVM]-G-G-[AG]-T

NAME Hydroxymethylglutaryl-coenzyme A reductases signature 3

CONSENSUS A-[LIVM]-x-[STAN]-x(2)-[LI]-x-[KRNQ]-[GSA]-H-[LM]-x-[FYLH]

NAME Hydroxymethylglutaryl-coenzyme A reductases profile

NAME 3-hydroxyacyl-CoA dehydrogenase signature

CONSENSUS [DNE]-x(2)-[GA]-F-[LIVMFY]-x-[NTJ-R-x(3)-[PA]-[LIVMFY](2)-x(5)-

CONSENSUS [LIVMFYCTHLIVMFY]-x(2MGV]

NAME Malate dehydrogenase active site signature

CONSENSUS [LIVM]-T-[TRKMN]-L-D-x(2)-R-[STA]-x(3)-[LIVMFY]

NAME Malic enzymes signature

CONSENSUS F-x-[DV]-D-x(2)-G-T-[GSA]-x-[IV]-x-[LIVMA]-[GAST](2)-[LIVMF](2)

NAME Isocitrate and isopropylmalate dehydrogenases signature

CONSENSUS [NS]-[LIMYT]-[FYDN]-G-[DNT]-[IMVY]-x-[STGDN]-[DN]-x(2)-[SGAP]-x(3,4)-G-

CONSENSUS [STG]-[LIVMPA]-G-[LIVMF]

NAME 6-phosphogluconate dehydrogenase signature CONSENSUS [LIVM]-x-D-x(2)-[GA]-[NQS]-K-G-T-G-x-W

NAME Glucose-6-phosphate dehydrogenase active site CONSENSUS D-H-Y-L-G-K-[EQK]

NAME IMP dehydrogenase / GMP reductase signature

CONSENSUS [LIVM]-[RK] [LIVM]-G-[LIVM]-G-x G S-[LIVM]-C-x-T

NAME Bactenal quinoprotein dehydrogenases signature 1

CONSENSUS [DEN]-W-x(3)-G-[RK]-x(6)-[FYW]-S-x(4)-[LIVM]-N-x(2)-N-V-x(2)-L-[RK]

NAME Bacterial quinoprotein dehydrogenases signature 2

CONSENSUS W-x(4)-Y-D-x(3)-[DN]-[LrVMFY](4)-x(2)-G-x(2)-[STA]-P

NAME FMN-dependent alpha-hydroxy acid dehydrogenases active site CONSENSUS S-N-H-G-[AG]-R-Q

NAME GMC oxidoreductases signature 1

CONSENSUS [GA]-[RKN]-x-[LIV]-G(2)-[GST](2)-x-[LIVM}-N-x(3)-[FYWA]-x(2)-[PAG]-x(5)-

CONSENSUS [DNESH]

NAME GMC oxidoreductases signature 2

CONSENSUS [GS]-[PSTA]-x(2)-[ST]-P-x-[LIVM](2)-x(2)-S-G-[LIVM]-G

NAME Eukaryotic molybdopteπn oxidoreductases signature

CONSENSUS [GA]-x(3)-[KRNQHT]-x(l l,14)-[LIVMFYWS]-x(8)-[LIVMF]-x-C-x(2)-[DEN]-R-

CONSENSUS x(2)-[DE]

NAME Prokaryotic molybdoptenn oxidoreductases signature 1

CONSENSUS [STAN]-x-[CH]-x(2,3)-C-[STAG]-[GSTVMF]-x-C-x-[LIVMFY ]-x-[LIVMA]-x(3,4)-

CONSENSUS [DENQKHT]

NAME Prokaryotic molybdoptenn oxidoreductases signature 2

CONSENSUS [STA]-x-[STAC](2)-x(2)-[STA]-D-[LIVMY](2)-L-P-x-[STAC](2)-x(2)-E

NAME Prokaryotic molybdoptenn oxidoreductases signature 3

CONSENSUS A-x(3)-[GDT]-I-x-[DNQTK]-x-[DEA]-x-[LIVM]-x-[LIVMC]-x-[NS]-x(2)-[GS]-

CONSENSUS x(5)-A-x-[LIVM]-[ST] NAME Aldehyde dehydrogenases glutamic acid active site

CONSENSUS [LIVMFGA]-E-[LIMSTAC]-[GS]-G-[KNLM]-[SADN]-[TAPFV]

NAME Aldehyde dehydrogenases cysteine active site

CONSENSUS [FYLVA]-x(3)-G-[QE]-x-C-[LIVMGSTANC]-[AGCN]-x-[GSTADNEKR]

NAME Aspartate-semialdehyde dehydrogenase signature

CONSENSUS [LIVM]-[SADN]-x(2)-C-x-R-[LIVM]-x(4)-[GSC]-H-[STA]

NAME Glyceraldehyde 3-phosphate dehydrogenase active site CONSENSUS [ASV]-S-C-[NT]-T-x(2)-[LIM]

NAME N-acetyl-gamma-glutamyl-phosphate reductase active site

CONSENSUS [LIVM]-[GSA]-x-P-G-C-[FY]-[AVP]-T-[GA]-x(3)-[GTAC]-[LIVM]-x-P

NAME Gamma-glutamyl phosphate reductase signamre

CONSENSUS V-x(5)-A-[LIV]-x-H-I-x(2)-[HY]-tGS]-[ST]-x-H-tST]-tDE]-x-I

NAME Dihydrodipico nate reductase signature CONSENSUS E-[IV]-x-E-x-H-x(3)-K-x-D-x-P-S-G-T-A

NAME Dihydroorotate dehydrogenase signature 1

CONSENSUS [GS]-x(4)-[GK]-[STA]-[IVSTA]-[GT]-x(3)-[NQR]-x-G-[NH]-x(2)-P-[RT]

NAME Dihydroorotate dehydrogenase signamre 2

CONSENSUS [LIV](2)-[GSA]-x-G-G-[IV]-x-[STGN]-x(3)-[ACV]-x(6)-G-A

NAME Coproporphyπnogen III oxidase signature

CONSENSUS K-x-W-C-x(2)-[FYH](3)-[LIVM]-x-H-R-x-E-x-R-G-[LIVM]-G-G-[LIVM]-F-F-D

NAME Fumarate reductase / succmate dehydrogenase FAD-binding site CONSENSUS R-[ST]-H-[ST]-x(2)-A-x-G-G

NAME Acyl-CoA dehydrogenases signature 1

CONSENSUS [GAC]-[LIVM]-[ST]-E-x(2)-[GSAN]-G-[ST]-D-x(2)-[GSA]

NAME Acyl-CoA dehydrogenases signature 2

CONSENSUS [QDE]-x(2)-G-[GS]-x-G-[LIVMFY]-x(2)-[DEN]-x(4)-[KR]-x(3)-[DEN]

NAME Alamne dehydrogenase & pyridine nucleotide transhydrogenase signamre 1 CONSENSUS G-[LIVM]-P-x-E-x(3)-N-E-x(l,3)-R-V-A-x-[ST]-P-x-[GST]-V-x(2)-L-x-[KRH]-

CONSENSUS x-G

NAME Alamne dehydrogenase & pyndine nucleotide transhydrogenase signature 2 CONSENSUS [LIVM](2)-G-[GA]-G-x-A-G-x(2)-[SA]-x(3)-[GA]-x-[SG]-[LIVM]-G-A-x-V-

CONSENSUS x(3)-D

NAME Glu / Leu / Phe / Val dehydrogenases active site CONSENSUS [LIV]-X(2)-G-G-[SAG]-K-X-[GV]-X(3)-[DNST]-[PL]

NAME D-amino acid oxidases signature

CONSENSUS [LIVM](2)-H-[NHA]-Y-G-x-[GSA](2)-x-G-x(5)-G-x-A

NAME Pyπdoxaimne 5 '-phosphate oxidase signamre CONSENSUS [LIVF]-E-F-W-[QHG]-x(4)-R-[LIVM]-H-[DNE]-R

NAME Copper amine oxidase topaquinone signature

CONSENSUS [LIVM]-[LIVMA]-[LIVM]-x(4)-T-x(2)-N-Y-[DE]-[YN]

NAME Copper amine oxidase copper-binding site signature CONSENSUS T-x-G-x(2)-H-[LIVMF]-x(3)-E-[DE]-x-P

NAME Lysyl oxidase putative copper-binding region signature CONSENSUS W-E-W-H-S-C-H-Q-H-Y-H

NAME Delta l-pyrrohne-5-carboxylate reductase signamre

CONSENSUS [PALF]-x(2,3)-[LIV]-x(3)-[LIVM]-[STAC]-[STV]-x-[GAN]-G-x-T-x(2)-[AG]-

CONSENSUS [LIV]-x(2)-[LMF]-[DENQK]

NAME Dihydrofolate reductase signamre

CONSENSUS [LVAGC]-[LIF]-G-x(4)-[LIVMF]-P-W-x(4,5)-[DE]-x(3)-[FYIV]-x(3)-[SΗQ]

NAME Tetrahydrofolate dehydrogenase/cyclohydrolase signature 1

CONSENSUS [EQ]-X-[EQK]-[LIVM](2)-X(2)-[LΓVM]-X(2)-[LΓVMY]-N-X-[DN]-X(5)-[LΓVMF](3)- CONSENSUS: Q-L-P-[LV].

NAME: Tetrahydrofolate dehydrogenase/cyclohydrolase signature 2 CONSENSUS P-G-G-V-G-P-[MF]-T-[IV],

NAME- Oxygen oxidoreductases covalent FAD-bmding site.

CONSENSUS' P-X(10)-[DE]-[LIVM]-X(3)-[LIVM]-X(9)-[LIVM]-X(3)-[GSA]-[GST]-G-H.

NAME Pyridine nucleotide-disulphide oxidoreductases class-I active site. CONSENSUS- G-G-X-C-[LIVA]-X(2)-G-C-[LIVM]-P.

NAME Pyridine nucleotide-disulphide oxidoreductases class-II active site CONSENSUS: C-X(2)-C-D-[GA]-X(2,4)-[FY]-X(4)-[LIVM]-X-[LIVM](2)-G(3)-[DN]

NAME Respiratory-chain NADH dehydrogenase subunit 1 signature 1

CONSENSUS. G-[LIVMFY RS]-[LIVMAGP]-Q-X-[LIVMFY]-X-D-[AGIM]-[LIVMFTA]-K-[LVMYST]-

CONSENSUS [LIVMFYG]-x-[KR]-[EQG]

NAME Respiratory-chain NADH dehydrogenase subumt 1 signature 2

CONSENSUS: P-F-D-[LIVMFYQ]-[STAGPVM]-E-[GAC]-E-x-[EQ]-[LIVMS]-x(2)-G

NAME Respiratory-cham NADH dehydrogenase 20 Kd subumt signature.

CONSENSUS- [GN]-x-D-[KRST]-[LIVMF](2)-P-[IV]-D-[LIVMFYW](2)-x-P-x-C-P-[PT].

NAME Respiratory-cham NADH dehydrogenase 24 Kd subumt signature. CONSENSUS ^■ D-x(2)-F-[ST]-x(5)-C-L-G-x-C-x(2)-[GA]-P

NAME Respiratory chain NADH dehydrogenase 30 Kd subunit signature.

CONSENSUS. E-R-E-X(2)-[DE]-[LΓVMF](2)-X(6)-[HK]-X(3)-[KRP]-X-[LIVM]-[LIVMS].

NAME Respiratory chain NADH dehydrogenase 49 Kd subunit signamre. CONSENSUS [LIVMH]-H-[RT]-[GA]-x-E-K-[LIVMT]-x-E-x-[KRQ]

NAME. Respiratory-cham NADH dehydrogenase 51 Kd subumt signamre 1 CONSENSUS G-[AM]-G-[AR]-Y-[LIVM]-C-G-[DE](2)-[STA](2)-[LIM](2)-[EN]-S.

NAME Respiratory-chain NADH dehydrogenase 51 Kd subunit signamre 2. CONSENSUS. E-S-C-G-x-C-x-P-C-R-x-G.

NAME' Respiratory-chain NADH dehydrogenase 75 Kd subunit signamre 1. CONSENSUS- P-x(2)-C-[Y S]-x(7)-G-x-C-R-x-C.

NAME Respiratory-cham NADH dehydrogenase 75 Kd subunit signature 2. CONSENSUS C-P-x-C-[DE]-x-[GS](2)-x-C-x-L-Q.

NAME. Respiratory-cham NADH dehydrogenase 75 Kd subumt signature 3. CONSENSUS: R-C-[LIVM]-x-C-x-R-C-[LIVM]-x-[FY].

NAME Nitrite and sulfite reductases lron-sulfur/siroheme-binding site CONSENSUS: [STV]-G-C-x(3)-C-x(6)-[DE]-[LIVMF]-[GAT]-[LIVMF].

NAME: Uncase signature

CONSENSUS: L-x-[LV]-L-K-[ST]-T-x-S-x-F-x(2)-[FY]-x(4)-[FY]

NAME Heme-copper oxidase catalytic subunit, copper B binding region signamre. CONSENSUS: [YWG]-[LIVFYWTA](2)-[VGS]-H-[LNP]-x-V-x(44,47)-H-H.

NAME CO II and mtrous oxide reductase dinuclear copper centers signamre. CONSENSUS: V-x-H-x(33,40)-C-x(3)-C-x(3)-H-x(2)-M.

NAME: Cytochrome c oxidase subumt Vb, zinc bindmg region signamre. CONSENSUS: [LIVM](2)-[FY ]-x(10)-C-x(2)-C-G-x(2)-[FYl-K-L.

NAME Multicopper oxidases signamre 1.

CONSENSUS: G-x-[FY ]-x-[LrVMFYW]-x-[CST]-x(8)-G-[LM]-x(3)-[LIVMFYW].

NAME: Multicopper oxidases signamre 2. CONSENSUS: H-C-H-x(3)-H-x(3)-[AG]-[LM]

NAME: Peroxidases proximal heme-hgand signature.

CONSENSUS. [DET]-[LIVMTA]-x(2)-[LIVM]-[LIVMSTAG]-[SAG]-[LIVMSTAG]-H-[STA]-[LIVMFY].

NAME: Peroxidases active site signamre.

CONSENSUS: [SGATV]-x(3)-[LIVMA]-R-[LIVMA]-x-[FW]-H-x-[SAC] . NAME Catalase proximal heme-hgand signamre.

CONSENSUS. R-[LIVMFSTAN]-F-[GASTNP]-Y-x-D-[AST]-[QEH]

NAME: Catalase proximal active site signamre

CONSENSUS [IF]-x-[RH]-x(4)-[EQ]-R-x(2)-H-x(2)-[GAS]-[GASTF]-[GAST].

NAME Glutathione peroxidases selenocysteine active site

CONSENSUS: [GN]-[RKHNFYC]-x-[LIVMFC]-[LIVMF](2)-x-N-[VT]-x-[STC]-x-C-[GA]-x-T.

NAME: Glutathione peroxidases signature 2 CONSENSUS [LIV]-[AGD]-F-P-[CS]-[NG]-Q-F.

NAME: Lipoxygenases iron-binding region signature 1.

CONSENSUS. H-[EQ]-x(3)-H-x-[LM]-[NQRC]-[GST]-H-[LIVMSTAC](3)-E

NAME Lipoxygenases iron-binding region signature 2.

CONSENSUS: [LIVMA]-H-P-[LIVM]-x-[KRQ]-[LIVMF](2)-x-[AP]-H.

NAME: Extradiol ring-cleavage dioxygenases signature.

CONSENSUS: [GNTIV]-x-H-x(5,7)-[LIVMF]-Y-x(2)-[DENTA]-P-x-[GP]-x(2,3)-E

NAME: Intradiol nng -cleavage dioxygenases signamre.

CONSENSUS- [LIVM]-x-G-x-[LIVM]-x(4)-[GS]-x(2)-[LIVM]-x(4)-[LIVM]-[DE]-[LIVMFY]-

CONSENSUS: x(6)-G-x-[FY].

NAME. Indoleamme 2,3-dιoxygenase signamre 1. CONSENSUS: G-G-S-[AN]-[GA]-Q-S-S-x(2)-Q. Indoleamme 2,3-dιoxygenase signamre 2

CONSENSUS' [FY]-L-[DQ]-[DE]-[LIVM]-x(2)-Y-M-x(3)-H-[KR]

NAME Bacterial ring hydroxylatmg dioxygenases alpha-subumt signamre CONSENSUS C-x-H-R-[GA]-x(8)-G-N-x(5)-C-x-[FY]-H.

NAME Bacterial luciferase subunits signamre

CONSENSUS [GA]-[LIVM]-P-[LIVM]-x-[LIVMFY]-x-W-x(6)-[RK]-x(6)-Y-x(3)-[AR]

NAME ubiH/COQ6 monooxygenase family signature CONSENSUS H-P-[LIV]-[AG]-G-Q-G-x-N-x-G-x(2)-D

NAME Biopteπn-dependent aromatic amino acid hydroxylases signamre. CONSENSUS P-D-x(2)-H-[DE]-[LI]-[LIVMF]-G-H-[LIVMC]-P

NAME Copper type II, ascorbate-dependent monooxygenases signamre 1. CONSENSUS- H-H-M-x(2)-F-x-C.

NAME' Copper type II, ascorbate-dependent monooxygenases signamre 2. CONSENSUS : H-x-F-x(4)-H-T-H-x(2)-G

NAME: Tyrosinase CuA-binding region signamre

CONSENSUS. H-x(4,5)-F-[LIVMFTP]-x-[FW]-H-R-x(2)-[LM]-x(3)-E.

NAME: Tyrosinase and hemocyanins CuB-binding region signature. CONSENSUS: D-P-x-F-[LIVMFY ]-x(2)-H-x(3)-D.

NAME Fatty acid desaturases family 1 signamre. CONSENSUS G-E-x-[FY] -H-N-[FY]-H-H-x-F-P-x-D-Y

NAME: Fatty acid desaturases family 2 signature.

CONSENSUS [ST]-[SA]-x(3)-[QR]-[LI]-x(5,6)-D-Y-x(2)-[LIVMFY ]-[LIVM]-[DE].

NAME: Cytochrome P450 cysteine he e-iron ligand signature. CONSENSUS. [FW]-[SGNH]-x-[GD]-x-[RHPT]-x-C-[LIVMFAP]-[GAD]

NAME- Heme oxygenase signamre. CONSENSUS: L-L-V-A-H-A-Y-T-R.

NAME: Copper/Zinc superoxide dismutase signamre 1.

CONSENSUS: [GA]-[IFAT]-H-[LIVF]-H-x(2)-[GP]-[SDG]-x-[STAGD].

NAME: Copper/Zmc superoxide dismutase signamre 2. CONSENSUS: G-[GN]-[SGA]-G-x-R-x-[SGA]-C-x(2)-[IV] . NAME Manganese and iron superoxide dismutases signamre CONSENSUS D-x-W-E-H-[STA]-[FY](2)

NAME Ribonucleotide reductase large subumt signamre

CONSENSUS W-x(2)-[LF]-x(6,7)-G-[LIVM]-[FYRA]-[NH]-x(3)-[STAQLIVM]-[ASC]-x(2)-

CONSENSUS [PA]

NAME Ribonucleotide reductase small subunit signamre

CONSENSUS [IVMSEQ]-E-x( 1 ,2)-[LIVTA]-[HY]-[GSA]-x-[STAVM]-Y-x(2)-[LIVMQ]-x(3)-

CONSENSUS [LIFYHIVFYCSA]

NAME Nitrogenases component 1 alpha and beta subunits signamre 1 CONSENSUS [LIVMFYH]-[LIVMFST]-H-[AG]-[AGSP]-[LIVMNQA]-[AG]-C

NAME Nitrogenases component 1 alpha and beta subunits signamre 2

CONSENSUS [STANQ]-[ET]-C-x(5)-G-D-[DN]-[LIVMT]-x-[STAGR]-[LIVMFYST]

NAME NifH/frxC family signature 1

CONSENSUS E-x-G-G-P-x(2)-[GA]-x-G-C-[AG]-G

NAME NifH/frxC family signamre 2

CONSENSUS D-x-L-G-D-V-V-C-G-G-F-[AG]-x-P

NAME Nickel-dependent hydrogenases large subumt signamre 1 CONSENSUS R-G-[LIVMF]-E-x(15)-[QESM]-R-x-C-G-[LIVM]-C

NAME Nickel-dependent hydrogenases large subumt signamre 2 CONSENSUS [FY]-D-P-C-[LIM]-[ASG]-C-x(2,3)-H

NAME Glutamyl-tRNA reductase signamre

CONSENSUS H-[LIVM]-x(2)-[LIVM]-[GSTAC](3)-[LIVM]-[DEQ]-S-[LrVMA]-[LIVM](2)-[GF]-E-

CONSENSUS x-[QR]-[IV]-[LIT]-[STAG]-Q-[LIVM]-[KR]

NAME Bactenal-type phytoene dehydrogenase signamre

CONSENSUS [NG]-x-[FYWV]-[LIVMF]-x-G-[AGC]-[GS]-[TA]-[HQT]-P-G-[STAV]-G-[LIVM]-

CONSENSUS x(5)-[GS]

NAME Glycme radical signamre

CONSENSUS [STIV]-x-R-[IVT]-[CSA]-G-Y-x-[GACV]

NAME Ergosterol biosynthesis ERG4/ERG24 family signamre 1 CONSENSUS G-x(2)-[LIVM]-Y-D-x-[FY]-x-G-x(2)-L-N-P-R

NAME Ergosterol biosynthesis ERG4/ERG24 family signamre 2 CONSENSUS [LIVM] (2)-H-R-x(2)-R-D-x(3)-C-x(2)-K-Y-G

NAME NNMT/PNMT/TEMT family of methyltraπsferases signamre CONSENSUS L-I-D-I-G-S-G-P-T-[IV]-Y-Q-L-L-S-A-C

NAME RNA methyltransferase trmA family signamre 1

CONSENSUS [DN]-P-[PA]-R-x-G-x(14, 16)-[LIVM](2)-Y-x-S-C-N-x(2)-T

NAME RNA methyltransferase trmA family signamre 2 CONSENSUS [LrVMF]-D-x-F-P-[QHY]-[ST]-x-H-[LIVMFY]-E

NAME Thymidylate synthase active site

CONSENSUS R-x(2)-[LIVM]-x(3)-[FW]-[QN]-x(8,9)-[LV]-x-P-C-[HAVM]-x(3)-[QMT]-[FYW]-

CONSENSUS x-[LV]

NAME Ribosomal RNA ademne dunethylases signamre

CONSENSUS [LIVM]-[LrVMFY]-[DE]-x-G-[STAPV]-G-x-[GA]-x-[LrVMF]-[ST]-x(2)-[LIVM]-

CONSENSUS x(6)-[LIVMY]-x-[STAGV]-[LIVMFYHC]-E-x-D

NAME Methylated-DNA--proteιn-cysteιne methyltransferase active site CONSENSUS [LrVMF]-P-C-H-R-[LIVMF](2)

NAME N-6 Adenine-specific DNA methylases signamre CONSENSUS [LIVMAC]-[LIVFYWA]-x-[DN]-P-P-[FYW]

NAME N-4 cytosine-specific DNA methylases signamre CONSENSUS [LIVMF]-T-S-P-P-[FY]

NAME C-5 cytosine-specific DNA methylases active site

CONSENSUS [DENKS]-x-[FLIV]-x(2)-[GSTC]-x-P-C-x(2)-[FY LIM]-S NAME C-5 cytosine-specific DNA methylases C-terminal signamre

CONSENSUS [RKQGTF]-x(2)-G-N-[STAG]-[LIVMF]-x(3)-[LIVMT]-x(3)-[LIVM]-x(3)-[LIVM]

NAME Proteιn-L-ιsoaspartate(D-aspartate) O-methyltransferase signamre CONSENSUS [GSA]-D-G-x(2)-G-[FYWV]-x(3)-[AS]-P-[FY]-[DN]-x-I

NAME Uropo hyπn-III C-methyltransferase signamre 1

CONSENSUS [LIVM]-[GS]-[STAL]-G-P-G-x(3)-[LIVMFY]-[LIVM]-T-[LIVM]-[KRHQG]-[AG]

NAME Uroporphyπn-III C-methyltransferase signamre 2

CONSENSUS V-x(2)-[LI]-x(2)-G-D-x(3)-[FYW]-[GS]-x(8)-[LIVF]-x(5,6)-[LIVMFY PAC]-

CONSENSUS x-[LIVMY]-x-P-G

NAME ubιE/C0Q5 methyltransferase family signamre 1 CONSENSUS Y-D-x-M-N-x(2)-[LIVM]-S-x(3)-H-x(2)-W

NAME ubiE/COQ5 methyltransferase family signamre 2

CONSENSUS R-V-[LIVM]-K-[PV]-G-G-x-[LIVMF]-x(2)-[LIVM]-E-x-S

NAME Senne hydroxymethyltransferase pyπdoxal-phosphate attachment site

CONSENSUS [DEH]-[LIVMFY]-x-[STMV]-[GST]-[ST](2)-H-K-[ST]-[LF]-x-G-[PAC]-[RQ]-

CONSENSUS [GSA]-[GA]

NAME Phosphonbosylglycinamide formyltransferase active site

CONSENSUS G-x-[STM]-[IVT]-x-[FY VQ]-[VMAT]-x-[DEVM]-x-[LIVMY]-D-x-G-x(2)-[LIVTJ-

CONSENSUS x(6)-[LIVM]

NAME Aspartate and ornithine carbamoyltransferases signamre CONSENSUS F-x-[EK]-x-S-[GT]-R-T

NAME Transketolase signamre 1

CONSENSUS R-x(3)-[LIVMTA]-[DENQSTHKF]-x(5,6)-[GSN]-G-H-[PLIVMF]-[GSTA]-x(2)-

CONSENSUS [LIMC]-[GS]

NAME Transketolase signamre 2

CONSENSUS G-[DEQGSA]-[DN]-G-[PAEQ]-[ST]-[HQ]-x-[PAGM]-[LιVMYAC]-[DEFY ]-x(2)-

CONSENSUS [STAP]-x(2)-[RGA]

NAME Transaldolase signamre 1

CONSENSUS [DG]-[rVSA]-T-[ST]-N-P-[STA]-[LIVMF](2)

NAME Transaldolase active site

CONSENSUS [LIVM]-x-[LrVM]-K-[LIVM]-[PAS]-x-[ST]-x-[DENQPAS]-G-[LIVM]-x-[AGV]-x-

CONSENSUS [QEKRST]-x-[LIVM]

NAME Acyltransferases ChoActase / COT / CPT family signamre 1

CONSENSUS [LI]-P-x-[LVP]-P-[IVTA]-P-x-[LrVM]-x-[DENQAS]-[ST]-[LIVM]-x(2)-[LY]

NAME Acyltransferases ChoActase / COT / CPT family signamre 2

CONSENSUS R-[FYW]-x-[DA]-[KA]-x(0,l)-[LrVMFY]-x-[LIVMFY](2)-x(3)-[DNS]-[GSA]-x(6)-

CONSENSUS [DE]-[HS]-x(3)-[DE]-[GA]

NAME Thiolases acyl-enzyme intermediate signamre

CONSENSUS [LIVM]-[NST]-x(2)-C-[SAGLI]-[ST]-[SAG]-[LIVMFYNS]-x-[STAG]-[LIVM]-x(6)-

CONSENSUS [LIVM]

NAME Thiolases signamre 2

CONSENSUS N-x(2)-G-G-x-[LIVM]-[SA]-x-G-H-P-x-G-x-[ST]-G

NAME Thiolases acnve site

CONSENSUS [AG]-[LIVMA]-[STAGLIVM]-[STAG]-[LIVMA]-C-x-[AG]-x-[AG]-x-[AG]-x-[SAG]

NAME Chloramphenicol acetyltransferase acnve site CONSENSUS Q-[LIV]-H-H-[SA]-x(2)-D-G-[FY]-H

NAME Hexapeptide-repeat contaimng-transferases signamre

CONSENSUS [LιV]-[GAED]-x(2)-[STAV]-x-[LIV]-x(3)-[LIVAC]-x-[LIV]-[GAED]-x(2)-

CONSENSUS [STAVR]-x-[LIV]-[GAED]-x(2)-[STAV]-x-[LIV]-x(3)-[LIV]

NAME Beta-ketoacyl synthases active site

CONSENSUS G-x(4)-[LIVMFAP]-x(2)-[AGC]-C-[STA](2)-[STAG]-x(3)-[LIVMF]

NAME Chalcone and stilbene synthases active site CONSENSUS R-[LIVMFYS]-x-[LIVM]-x-[QHG]-x-G-C-[FYNA]-[GA]-G-[GA]-[STAV]-x-[LIVMF]-

CONSENSUS [RA]

NAME Myπstoyl-CoA protein N-myπstoyltransferase signamre 1 CONSENSUS E-I-N-F-L-C-x-H-K

NAME Myπstoyl-CoA protein N-myπstoyltransferase signamre 2 CONSENSUS K-F-G-x-G-D-G

NAME Gamma-glutamyltranspeptidase signamre

CONSENSUS T-[STA]-H-x-[ST]-[LIVMA]-x(4)-G-[SN]-x-V-[STA]-x-T-x-T-[LIVM]-[NE]-

CONSENSUS x(l,2)-[FY]-G

NAME Transglutaminases active site

CONSENSUS [GT]-Q-[CA]-W-V-x-[SA]-[GA]-[IVT]-x(2)-T-x-[LMSC]-R-[CSA]-[LV]-G

NAME Phosphorylase pyπdoxal-phosphate attachment site CONSENSUS E-A-[SC]-G-x-[GS]-x-M-K-x(2)-[LM]-N

NAME UDP-glycosyltransferases signamre

CONSENSUS [F ]-x(2)-Q-x(2)-[LIVMYA]-[LIMV]-x(4,6)-[LVGAC]-[LVFYA]-[LIVMF]-[STAGCM]- CONSENSUS [HNQ]-[STAGC]-G-x(2)-[STAG]-x(3)-[STAGL]-[LIVMFA]-x(4)-[PQR]-[LIVMT]- CONSENSUS x(3)-[PA]-x(3)-[DES]-[QEHN]

NAME Punne/pyπmidine phosphoπbosyl transferases signamre

CONSENSUS [LIVMFY CTA]-[LIVM]-[LIVMA]-[LrVMFC]-[DE]-D-[LIVMS]-[LIVM]-[STAVD]-

CONSENSUS [STAR]-[GAC]-x-[STAR]

NAME Glutamine amidotransferases class-I active site

CONSENSUS [PAS]-[LIVMFYT]-[LIVMFY]-G-[LIVMFY]-C-[LIVMFYN]-G-x-[QEH]-x-[LIVMFA]

NAME Glutamine amidotransferases class-II active site CONSENSUS < x(0, 11 )-C-[GS]-[IV]-[LIVMFY ]-[AG]

NAME Purme and other phosphorylases family 1 signamre CONSENSUS [GST]-x-G-[LIVM]-G-x-[PA]-S-x-[GSTA]-I-x(3)-E-L

NAME Purme and other phosphorylases family 2 signamre

CONSENSUS [LIV]-x(3)-G-x(2)-H-x-[LIVMFY]-x(4) [LIVMF]-x(3)-[ATV]-x(l ,2)-[LIVM]-x-

CONSENSUS [ATV]-x(4)-[GN]-x(3,4)-[LIVMF](2)-x(2)-[STN]-[SA]-x-G-[GS]-[LIVM]

NAME Thymidine and pyπmidine-nucleoside phosphorylases signamre CONSENSUS S-[GS]-R-[GA]-[LIV]-x(2)-[TA]-[GA]-G-T-x-D-x-[LIV]-E

NAME ATP phosphoribosyltransferase signature

CONSENSUS E-x(5)-G-x-[SAG]-x(2)-[rV]-x-D-[LIV]-x(2)-[ST]-G-x-T-[LM]

NAME NAD arginine ADP-nbosyltransferases signamre CONSENSUS [FY]-x-[FY]-K-x(2)-H-[FY]-x-L-[ST]-x-A

NAME Prohpoprotein diacylglyceryl transferase signamre CONSENSUS G-R-x-[GA]-N-F-[LIVMF]-N-x-E-x(2)-G

NAME S-adenosylmethiomne synthetase signamre 1 CONSENSUS G-A-G-D-Q-G-x(3)-G-Y

NAME S-adenosylmethiomne synthetase signamre 2 CONSENSUS G-[GA]-G-[ASC]-F-S-x-K-[DE]

NAME Polyprenyl synthetases signamre 1

CONSENSUS [LIVM](2)-x-D-D-x(2,4)-D-x(4)-R-R-[GH]

NAME Polyprenyl synthetases signamre 2

CONSENSUS [LIVMFY]-G-x(2)-[FYL]-Q-[LIVM]-x-D-D-[LIVMFY]-x-[DNG]

NAME Squalene and phytoene synthases signamre 1

CONSENSUS Y-[CSAιM]-x(2)-[VSG]-A-[GSA]-[LIVAT]-[IV]-G-x(2)-[LMSC]-x(2)-[LIV]

NAME Squalene and phytoene synthases signamre 2

CONSENSUS [LIVM]-G-x(3)-Q-x(2,3)-N-[IF]-x-R-D-[LIVMFY]-x(2)-[DE]-x(4,7)-R-x-[FY]-

CONSENSUS x-P

NAME Protein prenyltransferases alpha subumt repeat signamre

CONSENSUS [PSIAV]-X-[NDFV]-[NEQIY]-X-[LΓVMAGP]-W-[NQSTHF]-[FYHQ]-[LIVMR] NAME Riboflavin synthase alpha chain family signamre

CONSENSUS [LΓVMF]-X(5)-G-[STADNQ]-[KREQIYW]-V-N-[LIVM]-E

NAME Dihydropteroate synthase signamre 1

CONSENSUS [LIVM]-x-[AG]-[LIVMF](2)-N-x-T-x-D-S-F-x-D-x-[SG]

NAME Dihydropteroate synthase signamre 2

CONSENSUS [GE]-[SA]-x-[LIVM](2)-D-[LIVM]-G-[GP]-x(2)-[STA]-x-P

NAME EPSP synthase signamre 1

CONSENSUS [LIVM]-x(2)-[GN]-N-[SA]-G-T-[STA]-x-R-x-[LIVMY]-x-[GSTA]

NAME EPSP synthase signamre 2

CONSENSUS [KR]-x-[KH]-E-[CST]-[DNE]-R-[LIVM]-x-[STA]-[LIVMC]-x(2)-[EN]-[LIVMF]-x-

CONSENSUS [KRA]-[LIVMF]-G

NAME FLAP/GST2/LTC4S family signamre CONSENSUS G-x(3)-F-E-R-V-[FY]-x-A-[NQ]-x-N-C

NAME Aminotransferases class-I pyndoxal-phosphate attachment site

CONSENSUS [GS]-[LIVMFYTAC]-[GSTA]-K-x(2)-[GSALVN]-[LIVMFA]-x-[GNAR]-x-R-[LIVMA]-

CONSENSUS [GA]

NAME Aminotransferases class-II pyndoxal-phosphate attachment site

CONSENSUS T-[LIVMFY ]-[STAG]-K-[SAG]-[LIVMFYWR]-[SAG]-x(2)-[SAG]

NAME Aminotransferases class-Ill pyndoxal-phosphate attachment site

CONSENSUS [LIVMFYWC](2)-x-D-E-[LIVMA] x(2)-[GP]-x(0,l)-[LIVMFYWAG]-x(0,l)-[SACR]-x-

CONSENSUS [GSAD]-x(12,16)-D-[LIVMFYWC]-x(2,3)-[GSA]-K-x(3)-[GSTADN]-[GSA]

NAME Aminotransferases class-IV signamre

CONSENSUS E-x-[STAGCI]-x(2)-N-[LIVMFAC]-[FY]-x(6,12)-[LIVMF]-x-T-x(6,8)-[LIVM]-x-

CONSENSUS [GS]-[LIVM]-x-[KR]

NAME Aminotransferases class-V pyndoxal-phosphate attachment site

CONSENSUS [LIVFYCHT]-[DGH]-[LIVMFYAC]-[LIVMFYA]-x(2)-[GSTAC]-[GSTA]-rHQR]-K-

CONSENSUS x(4,6)-G-x-[GSAT]-x-[LIVMFYSAC]

NAME Hexokinases signamre

CONSENSUS [LIVMJ-G-F [TN]-F-S-[FY]-P-x(5)-[LIVM]-[DNST]-x(3)-[LIVM]-x(2)-W-T-K-x-

CONSENSUS [LF]

NAME Galactokinase signamre

CONSENSUS G-R-x-N-[LIV]-I-G-E-H-x-D-Y

NAME GHMP kinases putative ATP-bindmg domain

CONSENSUS [LIVM]-[PK]-X-[GSTA]-X(0, 1)-G-L-[GS]-S-S-[GSA]-[GSTAC]

NAME Phosphofructokinase signature

CONSENSUS [RK]-x(4)-G-H-x-Q-[QR]-G-G-x(5)-D-R

NAME pfkB family of carbohydrate kinases signamre 1 CONSENSUS [AG]-G-x(0, l)-[GAP]-x-N-x-[STA]-x(6)-[GS]-x(9)-G

NAME pfkB family of carbohydrate kinases signamre 2

CONSENSUS [DNSK]-[PSTV]-x-[SAG](2)-[GD]-D-x(3)-[SAGV]-[AG]-[LIVMFY]-[LIVMSTAP]

NAME ROK family signamre

CONSENSUS [LIVM]-x(2)-G-[LIVMFCT]-G-x-[GA]-[LIVMFA]-x(8)-G-x(3,5)-[GATP]-x(2)-

CONSENSUS G-IRKH]

NAME Phosphoπbulokinase signamre

CONSENSUS K-[LIVM]-x-R-D-x(3)-R-G-x-[ST]-x-E

NAME Thymidine kmase cellular-type signamre

CONSENSUS [GA]-x(l,2)-[DE]-x-Y-x-[STAP]-x-C-[NKR]-x-[CH]-[LIVMFYWH]

NAME FGGY family of carbohydrate kinases signamre 1

CONSENSUS rMFYGS]-x-[PST]-x(2)-K-[LIVMFYW]-x- -[LIVMF]-x-[DENQTKR]-[ENQH]

NAME FGGY family of carbohydrate kinases signamre 2

CONSENSUS [GSA]-x-[LIVMFYW]-x-G-[LIVM]-x(7,8)-[HDENQ]-[LIVMF]-x(2)-[AS]-[STAIVM]-

CONSENSUS [LIVMFY]-[DEQ] NAME- Protein kinases ATP-binding region signamre

CONSENSUS. [LΓV]-G-{P}-G-{P}-[FYWMGSTNH]-[SGA]-{PW}-[LIVCAT]-{PD}-X-[GSTACLΓVMFY]-

CONSENSUS- x(5, 18)-[LIVMFYWCSTAR]-[AΓVP]-[LIVMFAGCKR]-K.

NAME: Seπne/Threonine protein kinases active-site signamre

CONSENSUS: [LrVMFYC]-x-[HY]-x-D-[LIVMFY]-K-x(2)-N-[LIVMFYCT](3)

NAME: Tyrosine protein kinases specific active-site signamre

CONSENSUS [LIVMFYC]-x-[HY]-x-D-[LIVMFY]-[RSTAC]-x(2)-N-[LIVMFYC](3).

NAME Protein kinase domain profile

NAME' Casein kinase II regulatory subunit signamre.

CONSENSUS: C-P-x-[LIVMY]-x-C-x(5)-L-P-[LIVMC]-G-x(9)-V-[KR]-x(2)-C-P-x-C.

NAME: Pyruvate kinase active site signamre

CONSENSUS: [LIVAC]-x-[LIVM](2)-[SAPCV]-K-[LIV]-E-[NKRST]-x-[DEQH]-[GSTA]-[LIVM]

NAME Shikimate kinase signamre

CONSENSUS: [KR]-x(2)-E-x(3)-[LIVMF]-x(8,12)-[LIVMF](2)-[SA]-x-G(3)-x-[LIVMF]

NAME Prokaryotic diacylglycerol kinase signamre CONSENSUS E-x-[LIVM]-N-[ST]-[SA]-[LIV]-E-x(2)-V-D.

NAME Phosphatidylinositol 3- and 4-kmases signamre 1

CONSENSUS [LIVMFAC]-K-x(l ,3)-[DEA]-[DE]-[LIVMC]-R-Q-[DE]-x(4)-Q.

NAME- Phosphatidylinositol 3- and 4-kmases signamre 2.

CONSENSUS [GS]-x-[AV]-x(3)-[LIVM]-x(2)-[FYH]-[LIVM](2)-x-[LIVMF]-x-D-R-H-x(2)-N

NAME: Acetate and butyrate kinases family signamre 1 CONSENSUS [LIVM](2)-x-[LIVM]-N-x-G-S-[ST]-S-x-[KE].

NAME: Acetate and butyrate kinases family signamre 2

CONSENSUS: [LIVMA](2)-x(2)-H-x-G-x-G-x-[ST]-[LIVM]-x-[AV]-x(3)-G Phosphoglycerate kinase signamre

CONSENSUS: [KRHGTCV]-[VT]-[LIVMF]-[LIVMC]-R-x-D-x-N-[SACV]-P.

NAME: Aspartokinase signamre

CONSENSUS- [LIVM]-x-K-[FY]-G-G-[ST]-[SC]-[LIVM]

NAME Glutamate 5-kιnase signamre

CONSENSUS: [GSTN]-x(2)-G-x-G-[GC]-[IM]-x-[STA]-K-[LIVM]-x-[SA]-[TCA]-x(2)-[GALV]-

CONSENSUS: x(3)-G

NAME ATP.guanido phosphotransferases active site CONSENSUS: C-P-x(0, 1)-[ST]-N-[IL]-G-T.

NAME PTS HPR component histidine phosphorylation site signamre CONSENSUS: G-[LIVM]-H-[STA]-R-[PA]-[GSTA]-[STAM] .

NAME PTS HPR component serine phosphorylation site signamre

CONSENSUS [GSADE]-[KREQTV]-x(4)-[KRN]-S-[LIVMF](2)-x-[LIVM]-x(2)-[LIVM]-[GAD]

NAME PTS EIIA domains phosphorylation site signamre 1 CONSENSUS: G-x(2)-[LIVMF](3)-H-[LIVMF]-G-[LrVMF]-x-T-[ALV] .

NAME PTS EIIA domains phosphorylation site signamre 2

CONSENSUS: [DENQ]-x(6)-[LIVMF]-[GA]-x(2)-[LIVM]-A-[LIVM]-P-H-[GAC].

NAME: PTS EIIB domains cysteine phosphorylation site signamre

CONSENSUS: N-[LIVMFY]-x(5)-C-x-T-R-[LIVMF]-x-[LIVMF]-x-[LIVM]-x-[DQ]

NAME: Adenylate kinase signamre.

CONSENSUS: [LrVMFYW](3)-D-G-[FYI]-P-R-x(3)-[NOJ .

NAME: Nucleoside diphosphate kinases active site CONSENSUS. N-x(2)-H-[GA]-S-D-[SA]-[LIVMPKNE] .

NAME: Guanylate kinase signamre.

CONSENSUS: T-[ST]-R-x(2)-[KR]-x(2)-[DE]-x(2)-G-x(2)-Y-x-[FY]-[LIVMK]. NAME Guanylate kinase domam profile

NAME Phosphoπbosyl pyrophosphate synthetase signamre

CONSENSUS D-[LI]-H-[SA]-x-Q-[IMST]-[QM]-G-[FY]-F-x(2)-P-[LIVMFC]-D

NAME 7,8-dιhydro-6-hydroxymethylpteπn-pyrophosphokιnase signamre CONSENSUS G-[PE]-R-x(2)-D-L-D-[LIVM](2)

NAME Bacteπophage-type RNA polymerase family active site signamre 1 CONSENSUS P-[LIVM]-x(2)-D-[GA]-[ST]-[AC]-[SN]-[GA]-[LIVMFY]-Q

NAME Bacteπophage-type RNA polymerase family active site signamre 2 CONSENSUS [LIVMF]-x-R-x(3)-K-x(2)-[LIVMF]-M-[PT]-x(2)-Y

NAME Eukaryotic RNA polymerase II heptapeptide repeat CONSENSUS Y-[ST]-P-[ST]-S-P-[STANK]

NAME RNA polymerases beta chain signamre

CONSENSUS G-x-K-[LιVMFA]-[STAC]-[GSTN]-x-[HSTA]-[GS]-[QNH]-K-G-[IVT]

NAME RNA polymerases M / 15 Kd subunits signamre

CONSENSUS F-C-x-[DEKST]-C-[GNK]-[DNSA}-[LIVMH]-[LIVM]-x(8,14)-C-x(2)-C

NAME RNA polymerases D / 30 to 40 Kd subunits signamre

CONSENSUS N-[SGA]-[LIVMF]-R-R-x(9)-[SA]-x(3)-V-x(4)-N-x-[STA]-x(3)-[DN]-E-x-[Ln-

CONSENSUS [GA]-x-R-[LI]-[GA]-[LIVM](2)-P

NAME RNA polymerases H / 23 Kd subunits signamre CONSENSUS H-[NEI]-[LIVM]-V-P-x-H-x(2)-[LIVM]-x(2)-[DE]

NAME RNA polymerases K / 14 to 18 Kd subunits signamre CONSENSUS [ST]-x-[FY]-E-x-[AT]-R-x-[LIVM]-[GSA]-x-R-[SA]-x-Q

NAME RNA polymerases L / 13 to 16 Kd subunits signamre

CONSENSUS [DE](2)-H-[ST]-[LIVM]-[GAP]-N-x(l 1) V-x-[FM]-x(2)-Y-x(3)-H-P

NAME RNA polymerases N / 8 Kd subumts signamre CONSENSUS [LIVMF](2)-P-[LIVM]-x-C-F-[ST]-C-G

NAME DNA polymerase family A signamre

CONSENSUS R-x(2)-[GSAV]-K-x(3)-[LIVMFY]-[AGQ] x(2)-Y-x(2)-[GS]-x(3)-[LIVMA]

NAME DNA polymerase family B signamre

CONSENSUS [YA]-[GLIVMSTAC]-D-T-D-[SG]-[LIVMFTC]-x-[LIVMSTAC]

NAME DNA polymerase family X signamre

CONSENSUS G-[SG]-[LFY]-x-R-[GE]-x(3)-[SGCL]-x-D-[LIVM]-D-[LIVMFY](3)-x(2)-[SAP]

NAME Galactose- 1-phosphate uridyl transferase family 1 active site signamre CONSENSUS F-E-N-[RK]-G-x(3)-G-x(4)-H-P-H-x-Q

NAME Galactose- 1-phosphate uridyl transferase family 2 signature CONSENSUS D-L-P-I-V-G-G-[ST]-[LIVM](2)-[SA]-H-[DEN]-H-[FY]-Q-G-G

NAME ADP-glucose pyrophosphorylase signamre 1

CONSENSUS [AG]-G-G-x-G-[STK]-x-L-x(2)-L-[TA]-x(3)-A-x-P-A-[LV]

NAME ADP-glucose pyrophosphorylase signamre 2 CONSENSUS W-[FY]-x-G-[ST]-A-[DNSH]-[AS]-[LIVMFYW]

NAME ADP-glucose pyrophosphorylase signamre 3

CONSENSUS [APV]-[GS]-M-G-[LIVMN]-Y-[rVC]-[LIVMFY]-x(2)-[DENPHK]

NAME Phosphatidate cytidylyltransferase signamre

CONSENSUS S-x-[LIVMF]-K-R-x(4)-K-D-x-[GSA]-x(2)-[LI]-[PG]-x-H-G-G-[LIVM]-x-D-R-

CONSENSUS [LIVMFT]-D

NAME Ribonuclease PH signature

CONSENSUS C-[DE]-[LIVM](2)-Q-[GTA]-D-G-[SG]-x(2)-[TA]-A

NAME 2'-5'-olιgoadenylate synthetases signamre 1

CONSENSUS G-G-S-x-[AG]-[KR]-x-T-x-L-[KR]-[GST]-x-S-D-[AG]

NAME 2'-5'-olιgoadenylate synthetases signamre 2 CONSENSUS R-P-V-I-L-D-P-x-[DE]-P-T

NAME CDP-alcohol phosphatidyltransferases signamre CONSENSUS D-G-x(2)-A-R-x(8)-G-x(3)-D-x(3)-D

NAME PEP-utilizmg enzymes phosphorylation site signamre

CONSENSUS G-[GA]-x-[TN]-x-H-[STA]-[STAV]-[LIVM](2)-[STAV]-[RG]

NAME PEP-uti zing enzymes signamre 2

CONSENSUS [DEQS]-x-[LιVMF]-S-[LIVMF]-G-[ST]-N-D-[LIVM]-x-Q-[LrVMFYGT]-[STALIV]-

CONSENSUS [LIVMF]-[GAS]-x(2)-R

NAME Rhodanese signamre 1

CONSENSUS [FY]-x(3)-H-[LIV]-P-G-A-x(2)-[LIVF]

NAME Rhodanese C-terminal signamre

CONSENSUS [AV]-x(2)-[FY]-[DEAP]-G-[GSA]-[WF]-x-E-[FYW]

NAME CoA transferases signature 1

CONSENSUS [DN]-[GN]-x(2)-[LIVMFA](3)-G-G-F-x(3)-G-x-P

NAME CoA transferases signature 2

CONSENSUS [LF]-[HQ]-S-E-N-G-[LIVF](2)-[GA]

NAME Phospholipase A2 histidine active site CONSENSUS C-C-x(2)-H-x(2)-C

NAME Phospholipase A2 aspartic acid active site CONSENSUS [LIVMA]-C-{LrVMFYWPCST}-C-D-x(5)-C

NAME Lipases, serine active site

CONSENSUS [LIV]-x-[LIVFY]-[LIVMST]-G-[HYWV]-S-x-G-[GSTAC]

NAME Co pase signamre CONSENSUS Y-x(2)-Y-Y-x-C-x-C

NAME Lipolytic enzymes "G-D-S-L" family, serme active site CONSENSUS [LIVMFYAG](4)-G-D-S-[LIVM]-x(l ,2)-[TAG]-G

NAME Lipolytic enzymes "G-D-X-G" family, putative histidine active site CONSENSUS [LIVMF](2)-x-[LIVMF]-H-G-G-[SAG]-[FY]-x(3)-[STDN]-x(2)-[ST]-H

NAME Lipolytic enzymes "G-D-X-G" family, putative serine active site CONSENSUS [LrVM]-x-[LIVMF]-[SA]-G-D-S-[CA]-G-[GA]-x-L-[CA]

NAME Carboxylesterases type-B serine active site

CONSENSUS F-[GR]-G-x(4)-[LIVM]-x-[LIV]-x-G-x-S-[STAG]-G

NAME Carboxylesterases type-B signamre 2

CONSENSUS [ED]-D-C-L-[YT]-[LIV]-[DNS]-[LIV]-[LIVFYW]-x-[PQR]

NAME Pectinesterase signamre 1

CONSENSUS [GSTN]-x(5)-[LIVM]-x-[LIVM]-x(2)-G-x-Y-[DNK]-E-x-[LrVM]-x-[LIVM]

NAME Pectinesterase signamre 2 CONSENSUS G-[STAD]-[LIVMT]-D-F-I-F-G

NAME Peptidyl-tRNA hydrolase signamre 1

CONSENSUS [FY]-x(2)-T-R-H-N-x-G-x(2)-[LIVMFA](2)-[DE]

NAME Peptidyl-tRNA hydrolase signamre 2

CONSENSUS [GS]-x(3)-H-N-G-[LIVM]-[KR]-[DNS}-[LIVMT]

NAME Alkaline phosphatase active site

CONSENSUS [IV]-x-D-S-[GAS]-[GASC]-[GAST]-[GA]-T

NAME Histidine acid phosphatases phosphohistidme signamre

CONSENSUS [LIVM]-x(2)-[LIVMA]-x(2)-[LIVM]-x-R-H-[GN]-x-R-x-[PAS]

NAME Histidine acid phosphatases active site signamre

CONSENSUS [LIVMF]-x-[LIVMFAG]-x(2)-[STAGI]-H-D-[STANQ]-x-[LIVM]-x(2)-[LIVMFY]-x(2)-

CONSENSUS [STA]

NAME Class A bacterial acid phosphatases signamre CONSENSUS G-S-Y-P-S-G-H-T

NAME 5'-nucleotιdase signamre 1

CONSENSUS [LIVM]-x-[LIVM](2)-[HEA]-[TI]-x-D-x-H-[GSA]-x-[LrVMF]

NAME 5'-nucleotιdase signamre 2

CONSENSUS [FYP]-x(4)-[LIVM]-G-N-H-E-F-[DN]

NAME Fructose- 1-6-bιsphosphaιase active site

CONSENSUS [AG]-[RK]-L-x(l,2)-[LIV]-[FY]-E-x(2)-P-[LIVM]-[GSA]

NAME Serine/threomne specific protein phosphatases signamre CONSENSUS [LIVM]-R-G-N-H-E

NAME Protein phosphatase 2A regulatory subumt PR55 signamre 1 CONSENSUS E-F-D-Y-L-K-S-L-E-I-E-E-K-I-N

NAME Protein phosphatase 2A regulatory subumt PR55 signamre 2 CONSENSUS N-[AG]-H-[TA]-Y-H-I-N-S-I-S-[LIVM]-N-S-D

NAME Protem phosphatase 2C signamre

CONSENSUS [LIVMFY]-[LIVMFYA]-[GSAC]-[LIVM]-[FYC]-D-G-H-[GAV]

NAME Tyrosine specific protein phosphatases active site

CONSENSUS [LIVMF]-H-C-x(2)-G-x(3)-[STC]-[STAGP]-x-[LIVMFY]

NAME Tyrosine specific protein phosphatases profile

NAME Dual specificity protein phosphatase profile

NAME PTP type protein phosphatase profile

NAME Inositol monophosphatase family signamre 1

CONSENSUS [FWV]-x(0,l)-[LIVM]-D-P-[LIVM]-D-[SG]-[ST]-x(2)-[FY]-x-[HKRNSTY]

NAME Inositol monophosphatase family signamre 2

CONSENSUS [WV]-D-x-[AC]-[GSA]-[GSAPV]-x-[LIVACP]-[LIV]-[LIVAC]-x(3)-[GH]-[GA]

NAME Prokaryotic zinc-dependent phospholipase C signamre CONSENSUS H-Y-x-[GT]-D-[LIVM]-[DNS]-x-P-x-H-[PA]-x-N

NAME Phosphatidylinositol-specific phospholipase X-box domain profile

NAME Phosphatidylinositol-specific phospholipase Y-box domain profile

NAME 3'5'-cychc nucleotide phosphodiesterases signamre CONSENSUS H-D-[LIVMFY]-x-H-x-[AG]-x(2)-[NQ]-x-[LIVMFY]

NAME cAMP phosphodiesterases class-II signamre

CONSENSUS H-x-H-L-D-H-[LIVM]-x-[GS]-[LIVMA]-[LIVM](2)-x-S-[AP]

NAME Sulfatases signature 1

CONSENSUS [SAP]-[LIVMST]-[CS]-[STAC]-P-[STA]-R-x(2)-[LIVMFW](2)-[TR]-G

NAME Sulfatases signature 2

CONSENSUS G-[YV]-x-[ST]-x(2)-[IVA]-G-K-x(0, 1)-[FYWK]-[HL]

NAME AP endonucleases family 1 signamre 1 CONSENSUS [APF]-D-[LIVMF](2)-x-[LIVM]-Q-E-x-K

NAME AP endonucleases family 1 signamre 2

CONSENSUS D-[ST]-[FY]-R-[KH]-x(7,8)-[FYW]-[ST]-[FYW](2)

NAME AP endonucleases family 1 signamre 3

CONSENSUS N-x-G-x-R-[LrVM]-D-[LIVMFYH]-x-[LV]-x-S

NAME AP endonucleases family 2 signature 1

CONSENSUS H-x(2)-Y-[LIVMF]-[IM]-N-[LIVMCA]-[AG]

NAME AP endonucleases family 2 signamre 2 CONSENSUS [GR]-[LIVMF]-C-[LIVM]-D-T-C-H

NAME AP endonucleases family 2 signamre 3

CONSENSUS [LIVMW]-H-x-N-[DE]-[SA]-K-x(3)-G-[SA]-x(2)-D NAME Deoxyπbonuclease I signamre 1

CONSENSUS [LIVM](2)-[AP]-L-H-[STA](2)-P-x(5)-E-[LIVM]-[DN]-x-L-x-[DE]-V

NAME Deoxynbonuclease I signamre 2 CONSENSUS G-D-F-N-A-x-C-[SA]

NAME Endonuclease III iron-sulfur binding region signamre CONSENSUS C-x(3)-[KRS]-P-[KRAGL]-C-x(2)-C-x(5)-C

NAME Endonuclease III family signamre

CONSENSUS [GST]-x-[LιVMF]-P-x(5)-[LIVMW]-x(2,3)-[Lη-[PAS]-G-V-[GA]-x(3)-[GAC]-

CONSENSUS x(3)-[LIVM]-x(2)-[SALV]-[LIVMFYW]-[GANK]

NAME Ribonuclease II family signamre

CONSENSUS [HI]-[FYE]-[GSTAM]-[LIVM]-x(4,5)-Y-[STAL]-x-[FWVAC]-[TV]-[SA]-P-[LIVMA]-

CONSENSUS [RQ]-[KR]-[FY]-x-D-x(3)-[HQ]

NAME Ribonuclease III family signamre

CONSENSUS [DEQ]-[RQ]-[LM]-E-[FYW]-[LV]-G-D-[SAR]

NAME Bacterial Ribonuclease P protein component signamre

CONSENSUS [LIVMFYS]-x(2)-A-x(2)-R-[NH]-[KRQL]-[LIVM]-[KRA]-R-x-[LIVMTA]-[KR]

NAME Ribonuclease T2 family histidine active site 1 CONSENSUS [FYWL]-X-[LΓVM]-H-G-L-W-P

NAME Ribonuclease T2 family histidine active site 2

CONSENSUS [LIVMF]-x(2)-[HDGTY]-[EQ]-[FYW]-x-[KR]-H-G-x-C

NAME Pancreatic πbonuclease family signamre CONSENSUS C-K-x(2)-N-T-F

NAME DNA/RNA non-specific endonucleases active site CONSENSUS D-R-G-H-[QIL]-x(3)-A

NAME Thermonuclease family signamre 1

CONSENSUS D-G-D-T-[LIVM]-x-[LIVMC]-x(9,10)-R-[LIVM]-x(2)-[LIVM]-D-x-P-E

NAME Thermonuclease family signamre 2

CONSENSUS D [KR]-Y-[GQ]-R-x-[LV]-[GA]-x-[IV]-[FYW]

NAME Beta-amylase active site 1 CONSENSUS H-x-C-G-G-N-V-G-D

NAME Beta-amylase active site 2

CONSENSUS G-x-[SA]-G-E-[LIVM]-R-Y-P-S-Y

NAME Glucoa ylase active site region signature CONSENSUS [STN]-[GP]-x(l ,2)-[DE]-x-W-E-E-x(2)-[GS]

NAME Polygalacturonase active site

CONSENSUS [GSDENKRH]-x(2)-[VMFC]-x(2)-[GS]-H-G-[LIVMAG]-x(l,2)-[LIVM]-G-S

NAME Clostridium cellulosome enzymes repeated domain signamre

CONSENSUS D-[LIVMFY]-[DNV]-x-[DNS]-x(2)-[LIVM]-[DN]-[SALM]-x-D-x(3)-[LIVMF]-x-

CONSENSUS [RKS]-x-[LIVMF]

NAME Chitinases family 18 active site

CONSENSUS [LIVMFY]-[DN]-G [LIVMF]-[DN]-[LIVMF]-[DN]-x-E

NAME Chinnases family 19 signamre 1

CONSENSUS C-x(4,5)-F-Y-[ST]-x(3)-[FY]-[LIVMF]-x-A-x(3)-[YF]-x(2)-F-[GSA]

NAME Chitinases family 19 signamre 2

CONSENSUS [LΓVM]-[GSA]-F-X-[STAG](2)-[LIVMFY]-W-[FY]-W-[LIVM]

NAME Alpha-lactalbumm / lysozyme C signamre CONSENSUS C-x(3)-C-x(2)-[LMF]-x(3)-[DEN]-[LI]-x(5)-C

NAME Alpha-galactosidase signamre

CONSENSUS G-[LrVMFY]-x(2)-[LIVMFY]-x-[LIVM]-D-D-x-W-x(3,4)-R-[DNSF]

NAME Trehalase signamre 1 CONSENSUS: P-G-G-R-F-x-E-x-Y-x-W-D-x-Y

NAME Trehalase signamre 2.

CONSENSUS Q-W-D-x-P-x-[GA]-W-[PA]-P.

NAME Alpha-L-fucosidase putative active site. CONSENSUS : P-x(2)-L-x(3)-K-W-E-x-C .

NAME' Glycosyl hydrolases family 1 active site

CONSENSUS [LIVMFSTC]-[LIVFYS]-[LIV]-[LIVMST]-E-N-G-[LIVMFAR]-[CSAGN].

NAME: Glycosyl hydrolases family 1 N-terminal signamre

CONSENSUS: F-x-[FYWM]-[GSTA]-x-[GSTA]-x-[GSTA](2)-[FYNH]-[NQ]-x-E-x-[GSTA].

NAME Glycosyl hydrolases family 2 signamre 1

CONSENSUS. N-x-[LIVMFYWD]-R-[STACN](2)-H-Y-P-x(4)-[LIVMFYW](2)-x(3)-[DN]-x(2)-

CONSENSUS- G-[LIVMFYW](4).

NAME. Glycosyl hydrolases family 2 acid/base catalyst.

CONSENSUS [DENQF]-[KRVW]-N-H-[AP]-[SAC]-[LIVMF](3)-W-[GS]-x(2,3)-N-E

NAME- Glycosyl hydrolases family 3 active site.

CONSENSUS [LIVM](2)-[KR]-x-[EQK]-x(4)-G-[LIVMFT]-[LιVT]-[LIVMF]-[ST]-D-x(2)-

CONSENSUS [SGADNI].

NAME: Glycosyl hydrolases family 5 signamre

CONSENSUS [LIV]-[LIVMFYWGA](2)-[DNEQG]-[LIVMGST]-x-N-E-[PV]-[RHDNSTLIVFY].

NAME: Glycosyl hydrolases family 6 signamre 1

CONSENSUS. V-x-Y-x(2)-P-x-R-D-C-[GSAF]-x(2)-[GSA](2)-x-G.

NAME: Glycosyl hydrolases family 6 signamre 2

CONSENSUS. [LIVMYA]-[LIVA]-[LIVT]-[LIV]-E-P-D-[SAL]-[LI]-[PSAG]

NAME- Glycosyl hydrolases family 8 signamre.

CONSENSUS A-[ST]-D-[AG]-D-x(2)-[IM]-A-x-[SA]-[LIVM]-[LIVMG]-x-A-x(3)-[FW]

NAME Glycosyl hydrolases family 9 active sites signamre 1

CONSENSUS [STV]-x-[LIVMFY]-[STV]-x(2)-G-x-[NKR]-x(4)-[PLIVM]-H-x-R.

NAME Glycosyl hydrolases family 9 active sites signamre 2. CONSENSUS [FYW]-x-D-x(4)-[FYW]-x(3)-E-x-[STA]-x(3)-N-[STA]

NAME Glycosyl hydrolases family 10 active site.

CONSENSUS: [GTA]-x(2)-[LIVN]-x-[IVMF]-[ST]-E-[LIY]-[DN]-[LIVMF].

NAME: Glycosyl hydrolases family 11 active site signamre 1. CONSENSUS: [PSA]-[LQ]-x-E-Y-Y-[LIVM](2)-[DE]-x-[FYWHN]

NAME: Glycosyl hydrolases family 11 active site signamre 2

CONSENSUS- [LrVMF]-x(2)-E-[AG]-[YWG]-[QRFGS]-[SG]-[STAN]-G-x-[SAF].

NAME: Glycosyl hydrolases family 16 active sites

CONSENSUS E-[LIV]-D-[LIV]-x(0,l)-E-x(2)-[GQ]-[KRNF]-x-[PSTA].

NAME: Glycosyl hydrolases family 17 signamre

CONSENSUS [LIVM]-x-[LIVMFYWA](3)-[STAG]-E-[STA]-G-W-P-[STN]-x-[SAGQ].

NAME- Glycosyl hydrolases family 25 active sites signamre.

CONSENSUS D-[LIVM]-x(3)-[NQ]-[PG]-x(9,10)-G-x(4)-[LIVMFY](2)-K-x-[ST]-E-[GS]-x(2)-

CONSENSUS: Y-x-[DN].

NAME Glycosyl hydrolases family 31 active site. CONSENSUS- [GF]-[LIVMF]-W-x-D-M-[NSA]-E.

NAME: Glycosyl hydrolases family 31 signamre 2.

CONSENSUS: G-[AV]-D-[LIVMT]-C-G-[FY]-x(3)-[ST]-x(3)-L-C-x-R-W-x(2)-[LV]-[GS]-[SA]-

CONSENSUS. F-x-P-F-x-R-[DN].

NAME: Glycosyl hydrolases family 32 active site. CONSENSUS: H-x(2)-P-x(4)-[LIVM]-N-D-P-N-G

NAME: Glycosyl hydrolases family 35 putative active site. CONSENSUS: G-G-P-[LIVM](2)-x(2)-Q-x-E-N-E-[FY]. NAME Glycosyl hydrolases family 39 active site CONSENSUS W-x-F-E-x-W-N-E-P-[DN]

NAME Glycosyl hydrolases family 45 active site CONSENSUS [STA]-T-R-Y-[FYW]-D-x(5)-[CA]

NAME Prokaryotic transglycosylases signamre

CONSENSUS [LIVM]-x(3)-E-S-x(3)-[AP]-x(3)-S-x(5)-G-[LIVM]-[LIVMFYW]-x-[LIVMFY ]-

CONSENSUS x(4)-[SAG]

NAME Inosine-uπdine preferring nucleoside hydrolase family signamre CONSENSUS D-x-D-[PT]-[GA]-x-D-D-[TAV]-[VI]-A

NAME Alkylbase DNA glycosidases alkA family signamre

CONSENSUS G-I-G-x-W-[ST]-[AV]-x-[LIVMFY](2)-x-[LIVM]-x(8)-[MF]-x(2)-[ED]-D

NAME Foπnamidopyπmidine-DNA glycosylase signamre

CONSENSUS C-x(2,4)-C-x-[GTAQ]-x-[IV]-x(7)-R-[GSTAN]-[STA]-x-[FYI]-C-x(2)-C-Q

NAME Uracil-DNA glycosylase signamre

CONSENSUS [KR]-[LIV]-[LIVC]-[LIVM]-x-G-[QI]-D-P-Y

NAME S-adenosyl-L-homocysteine hydrolase signamre 1

CONSENSUS [CS]-N-x-[FYL]-S-[ST]-[QA]-[DEN]-x-[AV](2)-A-A-[LIV]-[SAV]

NAME S-adenosyl-L-homocysteine hydrolase signamre 2 CONSENSUS G-K-x(3)-[LIV]-x-G-Y-G-x-V-G-[KR]-G-x-A

NAME Cytosol aminopeptidase signamre CONSENSUS N-T-D-A-E-G-R-L

NAME Aminopeptidase P and proline dipeptidase signamre

CONSENSUS [HA]-[GSYR]-[LIVMT]-[SG]-H-x-[LIV]-G-[LIVM]-x-[IV]-H-[DE]

NAME Methiomne aminopepndase subfamily 1 signature

CONSENSUS [MFY]-x-G-H-G-[LIVMC]-[GSH]-x(3)-H-x(4)-[LIVM]-x-[HN]-[YWV]

NAME Methiomne aminopeptidase subfamily 2 signature

CONSENSUS [DA]-[LrVMY]-x-K-rLIVM]-D-x-G-x-[HQ]-[LIVM]-[DNS]-G-x(3)-[DN]

NAME Renal dipeptidase active site

CONSENSUS [LIVM]-E-G-[GA]-x(2)-[LIVMF]-x(6)-L-x(3)-Y-x(2)-G-[LIVM]-R

NAME Senne carboxypeptidases, serme active site CONSENSUS [LIVM]-x-[GTA]-E-S-Y-[AG]-[GS]

NAME Serine carboxypeptidases, histidine active site

CONSENSUS [LIVF]-x(2)-[LIVSTA]-x-[IVPST]-x-[GSDNQL]-[SAGV]-[SG]-H-x-[IVAQ]-P-x(3)-

CONSENSUS [PSA]

NAME Zinc carboxypeptidases, zinc-binding region 1 signamre

CONSENSUS [PK]-X-[LΓVMFY]-X-[LIVMFY]-X(4)-H-[STAG]-X-E-X-[LΓVM]-[STAG]-X(6)-

CONSENSUS [LIVMFYTA]

NAME Zinc carboxypeptidases, zinc-binding region 2 signamre CONSENSUS H-[STAG]-x(3)-[LIVME]-x(2)-[LIVMFYW]-P-[FYW]

NAME Senne proteases, trypsin family, histidine active site CONSENSUS [LIVM]-[ST]-A-[STAG]-H-C

NAME Serine proteases, trypsin family, senne active site

CONSENSUS [DNSTAGC]-[GSTAPIMVQH]-x(2)-G-[DE]-S-G-[GS]-[SAPHV]-[LIVMFYWH]-

CONSENSUS [LIVMFYSTANQH]

NAME Serme proteases, subnlase family, aspartic acid active site

CONSENSUS [STAIV]-x-[LIVMF]-[LrVM]-D-[DSTA]-G-[LIVMFC]-x(2,3)-[DNH]

NAME Serine proteases, subtilase family, histidine acnve site

CONSENSUS H-G-[STM]-x-rVIC]-[STAGC]-[GS]-x-[LIVMA]-[STAGCLV]-[SAGM]

NAME Serine proteases, subnlase family, serme active site CONSENSUS G-T-S-x-[SA]-x-P-x(2)-[STAVC]-[AG] NAME: Serine proteases, V8 family, histidine active site.

CONSENSUS [ST]-G-[LIVMFYW](3)-[GN]-x(2)-T-[LIVM]-x-T-x(2)-H.

NAME Serine proteases, V8 family, serine active site CONSENSUS. T-X(2)-[GC]-[NQ]-S-G-S-X-[LΓVM]-[FY]

NAME: Serine proteases, omptin family signamre 1. CONSENSUS W-T-D-x-S-x-H-P-x-T

NAME: Serine proteases, omptin family signamre 2.

CONSENSUS- A-G-Y-Q-E-[ST]-R-[FYW]-S-[FYW]-[TN]-A-x-G-G-[ST]-Y

NAME: Prolyl endopepndase family serine active site.

CONSENSUS. D-x(3)-A-x(3)-[LIVMFYW]-x(14)-G-x-S-x-G-G-[LIVMFYW](2).

NAME: Endopeptidase Clp senne active site.

CONSENSUS T-x(2)-[LIVMF]-G-x-A-[SAC]-S-[MSA]-[PAG]-[STA]

NAME: Endopeptidase Clp histidine acdve site.

CONSENSUS R-x(3)-[EAP]-x(3)-[LIVMFYT]-M-[LIVM]-H-Q-P.

NAME ATP-dependent serine proteases, Ion family, serine active site. CONSENSUS. D-G-[PD]-S-A-[GS]-[LIVMCA]-[TA]-[LIVM]

NAME: Eukaryotic thiol (cysteine) proteases cysteine active site CONSENSUS Q-x(3)-[GE]-x-C-[YW]-x(2)-[STAGC]-[STAGCV] .

NAME: Eukaryotic thiol (cysteine) proteases histidine active site.

CONSENSUS: [LIVMGSTAN]-x-H-[GSACE]-[LIVM]-x-[LIVMAT](2)-G-x-[GSADNH].

NAME. Eukaryotic thiol (cysteine) proteases asparagine active site

CONSENSUS- [FYCH]-ι^q-[LIVT]-x-[KRQAG]-N-[ST]-W-x(3)-[FY ]-G-x(2)-G-[LFYW]-

CONSENSUS: [LIVMFYG]-x-[LIVMF].

NAME. Ubiquitin carboxyl-terminal hydrolase family 1 cysteine active-site CONSENSUS: Q-x(3)-N-[SA]-C-G-x(3)-[LIVM](2)-H-[SA]-[LIVM]-[SA] .

NAME Ubiquitin carboxyl-terminal hydrolases family 2 signamre 1

CONSENSUS G-[LΓVMFY]-X(1,3)-[AGC]-[NASM]-X-C-[FYW]-[LIVMC]-[NST]-[SACV]-X-[LIVMS]-

CONSENSUS Q

NAME Ubiquitin carboxyl-terminal hydrolases family 2 signamre 2. CONSENSUS Y-X-L-X-[SAG]-[LIVMFT]-X(2)-H-X-G-X(4,5)-G-H-Y.

NAME Caspase family histidine active site

CONSENSUS H-x(2,4)-[SC]-x(4)-[LIVMF](2)-[ST]-H-G.

NAME: Caspase family cysteine active site. CONSENSUS. K-P-K-[LIVMF](4)-Q-A-C-[RQG]-G

NAME. Eukaryotic and viral aspartyl proteases active site

CONSENSUS [LIVMFGAC]-[LIVMTADN]-[LIVFSA]-D-[ST]-G-[STAV]-[STAPDENQ]-x-[LIVMFSTNC]-

CONSENSUS. x-[LIVMFGTA].

NAME: Neutral zinc metallopeptidases, zinc-bmding region signamre

CONSENSUS: [GSTALIVN]-x(2)-H-E-[LIVMFYW]-{DEHRKP}-H-x-[LIVMFYWGSPQ].

NAME: Matπxins cysteine switch.

CONSENSUS: P-R-C-[GN]-x-P-[DR]-[LIVSAPKQ]

NAME: Insuhnase family, zinc-binding region signamre

CONSENSUS G-x(8,9)-G-x-[STA]-H-[LIVMFY]-[LrVMC]-[DERN]-[HRKL]-[LMFAT]-x-[LFSTH]-x-

CONSENSUS [GSTAN]-[GST].

//

AC PS01016;

DE Glycoprotease family signamre

CONSENSUS: [KR]-[GSAT]-x(4)-[FYWHL]-[DQNGK]-x-P-x-[LIVMFY]-x(3)-H-x(2)-[AG]-H-

CONSENSUS: [LIVM].

NAME: Proteasome A-type subunits signamre

CONSENSUS: [FY]-x(4)-[STNV]-x-[FYW]-S-P-x-G-[RKH]-x(2)-Q-[LIVM]-[DE]-Y-[SAD]-x(2)-

CONSENSUS: [SAG]. NAME Proteasome B-type subunits signamre

CONSENSUS [LIVMA]-[GSA]-[LIVMF]-x-[FYLVGAC]-x(2)-[GSACFY]-[LIVMSTAC](3)-[GAC]-

CONSENSUS [GSTACV]-[DES]-x(15)-[RK]-x(12,13)-G-x(2)-[GSTA]-D

NAME Signal peptidases I serine active site CONSENSUS [GS]-x-S-M-x-[PS]-[AT]-[LF]

NAME Signal peptidases I lysine active site

CONSENSUS K-R-[LIVMSTA](2)-G-x-[PG]-G-[DE]-x-[LIVM]-x-[LIVMFY]

NAME Signal peptidases I signamre 3

CONSENSUS [LIVMFYW](2)-x(2)-G-D-[NH]-x(3)-[SND]-x(2)-[SG]

NAME Signal peptidases II signamre

CONSENSUS [GAF]-[GA]-[GAS]-[LIVM]-[GAS]-N-[LVMFG]-[LIVMFY]-D-R-[LIMFA]

NAME Peptidase family U32 signature

CONSENSUS E-x-F-x(2)-G-[SA]-[LIVM]-C-x(4)-G-x-C-x-[LIVM]-S

NAME Amidases signamre

CONSENSUS G-[GA]-S-S-[GS]-G-x-[GSA]-[GSAVY]-x-[LIVM]-[GSA]-x(6)-[GSA]-x-[GA]-x-D-

CONSENSUS x-[GA]-x-S-[LIVM]-R-x-P-[GSAC]

NAME Asparaginase / glutaminase active site signamre 1 CONSENSUS [LIVM]-x(2)-T-G-G-T-[IV]-[AGS]

NAME Asparaginase / glutaminase active site signamre 2 CONSENSUS G-x-[LIVM]-x(2)-H-G-T-D-T-[LIVM]

NAME Urease mckel ligands signamre

CONSENSUS T-[AY]-[GA]-[GAT]-[LIVM]-D-x-H-[LIVM]-H-x(3)-P

NAME Urease active site

CONSENSUS [LIVM](2)-[CT]-H-[HN]-L-x(3)-[LrVM]-x(2)-D-[LIVM]-x-F-A

NAME ArgE / dapE / ACY1 / CPG2 / yscS family signamre 1 CONSENSUS [LIV]-[GALMY]-[LIVMF]-x-[GSA]-H-x-D-[TV]-[STAV]

NAME ArgE / dapE / ACY1 / CPG2 / yscS family signamre 2

CONSENSUS [GSTAI]-[SANQ]-D-x-K-[GSACN]-x(2)-[LIVMA]-x(2)-[LIVMFY]-x(14,17)-[LIVM]-

CONSENSUS x-[LrVMF]-[LIVMSTAG]-[LIVMFA]-x(2)-[DNG]-E-E-x-[GSTN]

NAME Dihydroorotase signamre 1

CONSENSUS D-[LIVMFYWSAP]-H-[LIVA]-H-[LIVF]-[RN]-x-[PGN]

NAME Dihydroorotase signamre 2 CONSENSUS [GA]-[ST]-D-x-A-P-H-x(4)-K

NAME Beta-lactamase class-A active site

CONSENSUS [FY]-x-[LIVMFY]-x-S-[TV]-x-K-x(4)-[AGLM]-x(2)-[LC]

NAME Beta-lactamase class-C active site CONSENSUS F-E-[LIVM]-G-S-[LIVMG]-[SA]-K

NAME Beta-lactamase class-D active site

CONSENSUS [PA]-x-S-[ST]-F-K-[LIV]-[PAL]-x-[STA]-[LI]

NAME Beta-lactamases class B signamre 1

CONSENSUS [LI]-x-[STN]-[HN]-x-H-[GSTA]-D-x(2)-G-[GP]-x(7,8)-[GS]

NAME Beta-lactamases class B signature 2

CONSENSUS P-x(3)-[LIVM](2)-x-G-x-C-[LIVMF](2)-K

NAME Argmase family signamre 1

CONSENSUS [LIVMF]-G-G-x-H-x-[LIVMT]-[STAV]-x-[PAG]-x(3)-[GSTA]

NAME Arginase family signamre 2

CONSENSUS [LIVM](2)-x-[LIVMFY]-D-[AS]-H-x-D

NAME Arginase family signamre 3

CONSENSUS [ST]-[LrVMFY]-D-[LIVM]-D-x(3)-[PAQ]-x(3)-P-[GSA]-x(7)-G

NAME Adenosine and AMP deaπunase signamre CONSENSUS [SA]-[LIVM] [NGS]-[STA]-D-D-P

NAME Cytidine and deoxycytidylate deaminases zinc-binding region signature

CONSENSUS [CH]-[AGV]-E-x(2)-[LIVMFGAT]-[LIVM]-x(17,33)-P-C-x(2,8)-C-x(3)-[LIVM]

NAME GTP cyclohydrolase I signamre 1

CONSENSUS [EN]-[LIVM](2)-x(2)-[KRQN]-[DN]-[LIVM]-x(3)-[ST]-x-C-E-H-H

NAME GTP cyclohydrolase I signamre 2

CONSENSUS [SA]-x-[RK]-x-Q-[LIVM]-Q-E-[RN]-[LI]-[TSN]

NAME Nitnlases / cyanide hydratase signamre 1

CONSENSUS G-x(2)-[LIVMFY](2)-x-[IF]-x-E-x(2)-[LIVM]-x-G-Y-P

NAME Nitnlases / cya de hydratase active site signamre

CONSENSUS G-[GAQ]-x(2)-C-[WA]-E-[NH]-x(2)-[PST]-[LIVMFYS]-x-[KR]

NAME Inorganic pyrophosphatase signamre

CONSENSUS D-[SGDN]-D-[PE]-[LIVMF]-D-[LIVMGAC]

NAME Acylphosphatase signamre 1 CONSENSUS [LIV]-x-G-x-V-Q-G-V-x-[FM]-R

NAME Acylphosphatase signature 2

CONSENSUS G-[FYW]-[AVC]-[KRQAM]-N-x(3)-G-x-V-x(5)-G

NAME ATP synthase alpha and beta subunits signamre CONSENSUS P-[SAP]-[LIV]-[DNH]-x(3)-S-x-S

NAME ATP synthase gamma subunit signature CONSENSUS [IV]-T-x-E-x(2)-[DE]-x(3)-G-A-x-[SAKR]

NAME ATP synthase delta (OSCP) subunit signamre

CONSENSUS [LIVM]-x-[LiVMFYT]-x(3)-[LIVMT] [DENQK]-x(2)-[LIVM]-x-[GSA]-G-[LIVMFYGA]-

CONSENSUS x-[LIVM]-[KRHENQ]-x-[GSEN]

NAME ATP synthase a subumt signamre

CONSENSUS [STAGN]-x-[STAG]-[LIVMF]-R-L-x-[SAGV]-N-[LIVMT]

NAME ATP synthase c subunit signamre

CONSENSUS [GSTA]-R-[NQ]-P-x(10)-[LIVMFYW](2)-x(3)-[LrVMFYW]-x-[DE]

NAME E1-E2 ATPases phosphorylation site CONSENSUS D-K-T-G-T-[LI]-[TI]

NAME Sodium and potassium ATPases beta subunits signamre 1

CONSENSUS [FY ]-x(2)-[FYW]-x-[FYW]-[DN]-x(6)-[LIVM]-G-R-T-x(3)-W

NAME Sodium and potassium ATPases beta subunits signamre 2 CONSENSUS [RK]-x(2)-C-[RKQWI]-x(5)-L-x(2)-C-[SA]-G

NAME GDA1/CD39 family of nucleoside phosphatases signamre

CONSENSUS [LrVM]-x-G-x(2)-E-G-x-[FY]-x-[FW]-[LIVA]-[TAG]-x-N-[HY]

NAME Iodothyronine deiodinases active site CONSENSUS R-P-L-V-x-N-F-G-S-[CA]-T-C-P-x-F

NAME Cutinase, senne active site

CONSENSUS P-x-[STA]-x-[LIV]-[IVT]-x-[GS]-G-Y-S-[QL]-G

NAME Cunnase, aspartate and histidine active sites

CONSENSUS C-x(3)-D-x-[IV]-C-x-G-[GST]-x(2)-[LIVM]-x(2,3)-H

NAME DDC / GAD / HDC / TyrDC pyndoxal-phosphate attachment site

CONSENSUS S-[LIVMFYW]-x(5)-K-[LrVMFYWG](2)-x(3)-[LIVMFYW]-x-[CA]-x(2)-[LIVMFYWQ]-

CONSENSUS x(2)-[RK]

NAME Orn Lys/Arg decarboxylases family 1 pyndoxal-P attachment site CONSENSUS [STAV]-x-S-x-H-K-x(2)-[GSTAN](2)-x-[STA]-Q-[STA](2)

NAME Orn/DAP/Arg decarboxylases family 2 pyndoxal-P attachment site

CONSENSUS [FY]-[PA]-X-K-[SACV]-[NHCLFW]-X(4)-[LIVMF]-[LIVMTA]-X(2)-[LΓVMA]-X(3)-

CONSENSUS [GTE] NAME Orn DAP/Arg decarboxylases family 2 signamre 2

CONSENSUS [GS]-x(2,6)-[LIVMSCP]-x(2)-[LIVMF]-[DNS]-[LIVMCA]-G-G-G-[LIVMFY]-

CONSENSUS [GSTPCEQ]

NAME Orotidine 5 '-phosphate decarboxylase active site

CONSENSUS [LIVMFTA]-[LIVMF]-x-D-x-K-x(2)-D-I-[GP]-x-T-[LIVMTA]

NAME Phosphoenolpyruvate carboxylase active site 1 CONSENSUS [VT]-x-T-A-H-P-T-[EQ]-x(2)-R-[KRH]

NAME Phosphoenolpyruvate carboxylase active site 2 CONSENSUS [IV]-M-[LIVM]-G-Y-S-D-S-x-K-D-[STAG]-G

NAME Phosphoenolpyruvate carboxykinase (GTP) signamre CONSENSUS F-P-S-A-C-G-K-T-N

NAME Phosphoenolpyruvate carboxykinase (ATP) signamre CONSENSUS L-I-G-D-D-E-H-x-W-x-[DE]-x-G-[IV]-x-N

NAME Uroporphyπnogen decarboxylase signamre 1 CONSENSUS P-x-W-x-M-R-Q-A-G-R

NAME Uroporphyπnogen decarboxylase signamre 2

CONSENSUS G-F-[STAGCV]-[STAGC]-x-P-[FYW]-T-[LV]-x(2)-Y-x(2)-[AE]-[GK]

NAME Indole-3-glycerol phosphate synthase signamre

CONSENSUS [LIVMFY]-[LΓVMC]-X-E-[LIVMFYC]-K-[KRSP]-[STAK]-S-P-[ST]-X(3)-[LΓVMFYST]

NAME Ribulose bisphosphate carboxylase large chain active site CONSENSUS G-x-[DN]-F-x-K-x-D-E

NAME Fructose-bisphosphate aldolase class-I active site CONSENSUS [LIVM]-x-[LIVMFYW]-E-G-x-[LS]-L-K-P-[SN]

NAME Fructose-bisphosphate aldolase class-II signamre 1

CONSENSUS [FYVM]-x(l,3)-[LIVMH]-[APN]-[LIVM]-x(l,2)-[LIVM]-H-x-D-H [GACH]

NAME Fructose-bisphosphate aldolase class-II signamre 2 CONSENSUS [LIVM]-E-x-E-[LIVM]-G-x(2)-[GM]-[GSTA]-x-E

NAME Malate synthase signamre

CONSENSUS [KR]-[DENQ]-H-x(2)-G-L-N-x-G-x-W-D-Y-[LIVM]-F

NAME Hydroxymethylglutaryl-coenzyme A lyase active site CONSENSUS S-V-A-G-L-G-G-C-P-Y

NAME Hydroxymethylglutaryl-coenzyme A synthase active site CONSENSUS N-x-[DN]-[rV]-E-G-[IV]-D-x(2)-N-A-C-[FY]-x-G

NAME Citrate synthase signature

CONSENSUS G-[FYA]-[GA]-H-x-[IV]-x(l,2)-[RKT]-x(2)-D-[PS]-R

NAME Alpha-isopropylmalate and homocitrate synthases signamre 1 CONSENSUS L-R-[DE]-G-x-Q-x(10)-K

NAME Alpha-isopropylmalate and homocitrate synthases signamre 2 CONSENSUS [LIVMFW]-x(2)-H-x-H-[DN]-D-x-G-x-[GAS]-x-[GASLI]

NAME KDPG and KHG aldolases active site CONSENSUS G-[LIVM]-x(3)-E-[LIV]-T-[LF]-R

NAME KDPG and KHG aldolases Schiff-base forming residue CONSENSUS G-x(3)-[LIVMF]-K-[LF]-F-P-[SA]-x(3)-G

NAME Isocitrate lyase signamre CONSENSUS K-[KR]-C-G-H-[LMQ]

NAME Beta-eliminating lyases pyndoxal-phosphate attachment site CONSENSUS Y-x-D-x(3)-M-S-[GA]-K-K-D-x-[LIVM](2)-x-[LIVM]-G-G

NAME DNA photolyases class 1 signamre 1

CONSENSUS T-G-x-P-[LIVM](2)-D-A-x-M-[RA]-x-[LIVM]

NAME DNA photolyases class 1 signamre 2 CONSENSUS [DN]-R-x-R-[LIVM](2)-x-[STA](2)-F-[LIVMFA]-x-K-x-L-x(2,3)-W-[KRQ]

NAME DNA photolyases class 2 signamre 1 CONSENSUS F-x-E-E-x-[LIVM](2)-R-R-E-L-x(2)-N-F

NAME DNA photolyases class 2 signamre 2

CONSENSUS G-x-H-D-x(2)-W-x-E-R-x-[LIVM]-F-G-K-[LIVM]-R-[FY]-M-N

NAME Eukaryotic-type carbonic anhydrases signamre

CONSENSUS S-E-H-x-[LIVM]-x(4)-[FYH]-x(2)-E-[LIVM]-H-[LIVMFA](2)

NAME Prokaryotic-type carbonic anhydrases signamre 1 CONSENSUS C-[SA]-D-S-R-[LIVM]-x-[AP]

NAME Prokaryotic-type carbomc anhydrases signature 2

CONSENSUS [EQ]-Y-A-[LIVM]-x(2)-[LIVM]-x(4)-[LIVMF](3)-x-G-H-x(2)-C-G Fumarate lyases signamre CONSENSUS G-S-x(2)-M-x(2)-K-x-N

NAME Acomtase family signamre 1

CONSENSUS [LIVM]-x(2)-[GSACIVM]-x-[LIV]-[GTIV]-[STP]-C-x(0,l)-T-N-[GSTANI]-x(4)-

CONSENSUS [LIVMA]

NAME Acomtase family signamre 2

CONSENSUS G-x(2)-[LIVWPQ]-x(3)-[GAC]-C-[GSTAM]-[LIMPTA]-C-[LIMV]-[GA]

NAME Dihydroxy-acid and 6-phosphogluconate dehydratases signamre 1 CONSENSUS C-D-K-x(2)-P-[GA]-x(3)-[GA]

NAME Dihydroxy-acid and 6-phosphogluconate dehydratases signamre 2 CONSENSUS [SA]-L-[LIVM]-T-D-[GA]-R-[LIVMF]-S-[GA]-[GAV]-[ST]

NAME Dehydroquinase class I active site

CONSENSUS D-[LIVM]-[DE]-[LIVN]-x(18,20)-[LIVM](2)-x-[SC]-[NHY]-H-[DN]

NAME Dehydroquinase class II signature

CONSENSUS [LIVM]-[NQ]-G-P-N-[LV]-x(2)-L-G-x-R-[QED]-P-x(2)-[FY]-G

NAME Enolase signamre

CONSENSUS [LIV](3)-K-x-N-Q-I-G-[ST]-[LIV]-[ST]-[DE]-[STA]

NAME Senne/threonine dehydratases pyndoxal-phosphate attachment site

CONSENSUS [DESH]-x(4,5)-[STVG]-x-[AS]-[FYI]-K-[DLIFSA]-[RVMF]-[GA]-[LIVMGA]

NAME Enoyl-CoA hydratase/isomerase signamre

CONSENSUS [LIVM]-[STA]-X-[LIVM]-[DENQRHSTA]-G-X(3)-[AG](3)-X(4)-[LIVMST]-X-[CSTA]- CONSENSUS [DQHPHLΓVMFY]

NAME Imidazoleglycerol-phosphate dehydratase signamre 1

CONSENSUS [LΓVMY]-[DE]-X-H-H-X(2)-E-X(2)-[GCA]-[LIVM]-[STAC]-[LIVM]

NAME Imidazoleglycerol-phosphate dehydratase signamre 2 CONSENSUS G-x-[DN]-x-H-H-x(2)-E-[STAGC]-x-[FY]-K

NAME Tryptophan synthase alpha chain signamre

CONSENSUS [LIVM]-E-[LIVM]-G-x(2)-[FYC]-[ST]-[DE}-[PA]-[LIVMY]-[AGLη-[DE]-G

NAME Tryptophan synthase beta chain pyndoxal-phosphate attachment site CONSENSUS [LIVM]-x-H-x-G-[STA]-H-K-x-N

NAME Delta-aminolevulinic acid dehydratase active site CONSENSUS G-x-D-x-[LIVM](2)-[IV]-K-P-[GSA]-x(2)-Y

NAME Urocanase active site CONSENSUS F-Q-G-L-P-x-R-I-C-W

NAME Prephenate dehydratase signamre 1

CONSENSUS [Fι^-x-[LIV^-x(2)-[LIVM]-x(5)-PN]-x(5)-T-R-F-[LIVM ]-x-[LIVM]

NAME Prephenate dehydratase signamre 2 CONSENSUS [LIVM]-[ST]-[KR]-[LIVM]-E-[ST]-R-P

NAME Dihydrodipicolinate synthetase signamre 1 CONSENSUS [GSA]-[LIVM]-[LIVMFY]-x(2)-G-[ST]-[TG]-G-E-[GASNF]-x(6)-[EQ]

NAME Dihydrodipicolinate synthetase signamre 2

CONSENSUS Y-[DNS]-[LIVMF]-P-x(2)-[ST]-x(3)-[LIVM]-x(13, 14)-[LIVM]-x-[SGA]-[LIVMF]-

CONSENSUS K-[DEQAF]-[STAC]

NAME RsuA family of pseudouπdine synthase signamre CONSENSUS G-R-L-D-x(2)-[ST]-x-G-[LIVMF](4)-[ST]-[DNT]

NAME Cysteine synthase/cystathiomne beta-synthase P-phosphate attachment site CONSENSUS K-x-E-x(3)-[PA]-[STAGC]-x-S-[IVAP]-K-x-R-x-[STAG]-x(2)-[LIVM]

NAME Phenylalanine and histidine ammonia-lyases signamre

CONSENSUS G-[STG]-[LIVM]-[STG]-[AC]-S-G-[DH]-L-x-P-L-[SA]-x(2)-[SA]

NAME Porphobihnogen deaminase cofactor-binding site

CONSENSUS E-R-x-[LIVMFA]-x(3)-[LIVMF]-x-G-[GSA]-C-x-[IVT]-P-[LIVMF]-[GSA]

NAME Cys/Met metabolism enzymes pyndoxal-phosphate attachment site

CONSENSUS [DQ]-[LIVMF]-x(3)-[STAGC]-[STAGCI]-T-K-[FYWQ]-[LIVMF]-x-G-[HQ]-[SGNH]

NAME Glyoxalase I signamre 1

CONSENSUS [HQ]-[IVT]-x-[LIVFY]-x-[IV]-x(5)-[STA]-x(2)-F-[YM]-x(2,3)-[LMF]-G-[LMF]

NAME Glyoxalase I signamre 2

CONSENSUS G-[NTKQ]-x(0,5)-[GA]-[LVFY]-[GH]-H-[IVF]-[CGA]-x-[STAGL]-x(2)-[DNC]

NAME Cytochrome c and cl heme lyases signamre 1 CONSENSUS H-N-x(2)-N-E-x(2)-W-[NQKR]-x(4)-W-E Cytochrome c and c 1 heme lyases signamre 2 CONSENSUS P-F-D-R-H-D-W

NAME Adenylate cyclases class-I signature 1 CONSENSUS E-Y-F-G-[SA](2)-L-W-x-L-Y-K

NAME Adenylate cyclases class-I signamre 2

CONSENSUS Y-R-N-x-W-[NS]-E-[LIVM]-R-T-L-H-F-x-G

NAME Guanylate cyclases signamre

CONSENSUS G-V-[LIVM]-x(0,l)-G-x(5)-[FY]-x-[LIVM]-[FYW]-[GS]-[DNTHKW]-[DNT]-[IV]-

CONSENSUS [DNTA]-x(5)-[DE]

NAME Chons ate synthase signamre 1

CONSENSUS G-E-S H [GC]-x(2)-[LIVM]-[GTV]-x-[LIVM](2)-[DE]-G-x-[PV]

NAME Chonsmate synthase signature 2

CONSENSUS [GE]-R-[SA](2)-[SAG]-R-[EV]-[ST]-x(2)-[RH]-V-x(2)-G

NAME Chonsmate synthase signamre 3

CONSENSUS R-[SH]-D-[PSV]-[CSAV]-x(4)-[GAI]-x-[rVGSP]-[LIVM]-x-E-[STAH]-[LIVM]

NAME 6-pyruvoyl tetrahydropteπn synthase signamre 1 CONSENSUS C-N-N-x(2)-G-H-G-H-N-Y

NAME 6-pyruvoyl tetrahydropteπn synthase signamre 2 CONSENSUS D-H-K-N-L-D-x-D

NAME Ferrochelatase signamre

CONSENSUS [LIVMF](2)-x-S-x-H-[GS]-[LIVM]-P-x(4,5)-[DENQKR]-x-G-D-x-Y

NAME Alamne racemase pyndoxal-phosphate attachment site CONSENSUS V-x-K-A-[DN]-[GA]-Y-G-H-G

NAME Aspartate and glutamate racemases signature 1

CONSENSUS [T A]-[LiVM]-x-C-x(0, 1)-N-[ST]-[MSA]-[STH]-[LIVFYSTANK]

NAME Aspartate and glutamate racemases signamre 2

CONSENSUS [LIVM](2)-x-[AG]-C-T-[DEH]-[LIVMFY]-[PNGRS]-x-[LIVM]

NAME Mandelate racemase / muconate lactomzing enzyme family signamre 1 CONSENSUS A-x-[SAG](2)-[LIVM]-[DE]-x-A-x(2)-D-x(2)-[GA]-[KR]

NAME Mandelate racemase / muconate lactomzing enzyme family signamre 2 CONSENSUS G-x(7)-D-x(9)-A-x(14)-[LIVM]-E-[DENQ]-P-x(4)-[DENQ]

NAME Ribulose-phosphate 3-epιmerase family signamre 1

CONSENSUS [LIVMF]-H-[LIVMFY]-D-[LIVM]-x-D-x( 1 ,2)-[FY]-[LIVM]-x-N-x-[STAV]

NAME Ribulose-phosphate 3-epιmerase family signature 2

CONSENSUS [LIVMA]-x-[LIVM]-M-[ST]-[VS]-x-P-x(3)-G-Q-x-F-x(6)-[NK]-[LIVMC]

NAME Aldose 1-epιmerase putative active site CONSENSUS [NS]-x-T-N-H-x-Y-[FW]-N-[LI]

NAME Cyclophihn-type peptidyl-prolyl cis-trans isomerase signature

CONSENSUS [FY]-x(2)-[STCNLV]-x-F-H-[RH]-[LIVMN]-[LIVM]-x(2)-F-[LIVM]-x-Q-[AG]-G

NAME Cyclophihn-type peptidyl-prolyl cis-trans isomerase profile

NAME FKBP-type peptidyl-prolyl cis-trans isomerase signamre 1

CONSENSUS [LIVMC]-x-[YF]-x-[GVL]-x( 1 ,2)-[LFT]-x(2)-G-x(3)-[DE]-[STAEQK]-[STAN]

NAME FKBP-type peptidyl-prolyl cis-trans isomerase signamre 2

CONSENSUS [LIVMFY]-x(2)-[GA]-x(3 ,4)-[LIVMF]-x(2) [LIVMFHK]-x(2)-G-x(4)-[LIVMF]-

CONSENSUS x(3)-[PSGAQ]-x(2)-[AG]-[FY]-G

NAME FKBP-type peptidyl-prolyl cis-trans isomerase domain profile

NAME PpiC-type peptidyl-prolyl cis-trans isomerase signamre

CONSENSUS F-[GSADEI]-x-[LVAQ]-A-x(3)-[ST]-x(3,4)-[STQ]-x(3,5)-[GER]-G-x-[LrVM]-

CONSENSUS [GS]

NAME Tπosephosphate isomerase active site CONSENSUS [AV]-Y-E-P-[LIVM]-W-[SA]-I-G-T-[GK]

NAME Xylose isomerase signamre 1 CONSENSUS [LI]-E-P-K-P-x(2)-P

NAME Xylose isomerase signamre 2

CONSENSUS [FL]-H-D-x-D-[LIV]-x-[PD]-x-[GDE]

NAME Phosphomannose isomerase type I signamre 1 CONSENSUS Y-x-D-x-N-H-K-P-E

NAME Phosphomannose isomerase type I signamre 2

CONSENSUS H-A-Y-[LIVM]-x-G-x(2)-[LIVM]-E-x-M-A-x-S-D N-x-[LIVM]-R-A-G-x-T-P-K

NAME Phosphoglucose isomerase signamre 1

CONSENSUS [DENS]-x-[LIVM]-G-G-R-[FY]-S-[LIVMT]-x-[STA]-[PSAC]-[LIVMA]-G

NAME Phosphoglucose isomerase signamre 2

CONSENSUS [GS]-x-[LIVM]-[LIVMFYW]-x(4)-[FY]-[DN]-Q-x-G-V-E-x(2)-K

NAME Glucosamιne/galactosamιne-6-phosphate isomerases signamre

CONSENSUS [LIVM]-x(3)-G-x-[LIT]-x-[LIV]-x-[LIVM]-x-G-[LIVM]-G-x-[DEN]-G-H

NAME Phosphoglycerate mutase family phosphohistidme signamre CONSENSUS [LIVM]-x-R-H-G-[EQ]-x(3)-N

NAME Phosphoglucomutase and phosphomannomutase phosphoseπne signamre CONSENSUS [GSA]-[LIVM]-x-[LIVM]-[ST]-[PGA]-S-H-x-P-x(4)-[GNHE]

NAME Methylmalonyl-CoA mutase signamre

CONSENSUS R-I-A-R-N-[TQ]-x(2)-[LIVMFY](2)-x-[EQ]-E-x(4)-[KRN]-x(2)-D-P-x-[GSA]-

CONSENSUS G-S

NAME Terpene synthases signamre

CONSENSUS [DE]-G-S-W-x-G-x-W-[GA]-[LIVM]-x-[FY]-x-Y-[GA]

NAME Eukaryonc DNA topoisomerase I active site

CONSENSUS [DEN]-x(6)-[GS]-[IT]-S-K-x(2)-Y-[LIVM]-x(3)-[LIVM]

NAME Prokaryonc DNA topoisomerase I active site

CONSENSUS [EQ]-x-L-Y-[DEQT]-x(3, 12)-[LI]-[ST]-Y-x-R-[ST]-[DEQS]

NAME DNA topoisomerase II signamre CONSENSUS [LIVMA]-x-E-G-[DN]-S-A-x-[STAG] NAME: Aminoacyl-transfer RNA synthetases class-I signamre.

CONSENSUS: P-x(0,2)-[GSTAN]-[DENQGAPK]-x-[LIVMFP]-[HT]-[LIVMYAC]-G-[HNTG]-

CONSENSUS: [LIVMFYSTAGPC].

NAME: Aminoacyl-transfer RNA synthetases class-II signamre 1. CONSENSUS: [FYH]-R-x-[DE]-x(4,12)-[RH]-x(3)-F-x(3)-[DE].

NAME: Aminoacyl-transfer RNA synthetases class-II signamre 2

CONSENSUS: [GSTALVF]-{DENQHRKP}-[GSTA]-[LIVMF]-[DE]-R-[LIVMF]-x-[LIVMSTAG]-[LIVMFY].

NAME: WHEP-TRS domain signamre.

CONSENSUS: [QY]-G-[DNEA]-x-[LIV]-[KR]-x(2)-K-x(2)-[KRNG]-[AS]-x(4)-[LIV]-[DENK]-

CONSENSUS: x(2)-[IV]-x(2)-L-x(3)-K.

NAME: ATP-citrate lyase / succinyl-CoA hgases family signamre 1.

CONSENSUS: S-[KR]-S-G-[GT]-[LIVM]-[GST]-x-[EQ]-x(8,10)-G-x(4)-[LIVM]-[GA]-[LIVM]-G-

CONSENSUS: G D.

NAME: ATP-citrate lyase / succmyl-CoA hgases family active site. CONSENSUS: G-x(2)-A-x(4,7)-[RQT]-[LIVMF]-G-H-[AS]-[GH] .

NAME: ATP-citrate lyase / succinyl-CoA hgases family signamre 3.

CONSENSUS: G-x-[IV]-x(2)-[LIVMF]-x-[NA]-G-[GA]-G-[LA]-[STAV]-x(4)-D-x-[LIVM]-x(3)-

CONSENSUS: G-[GRE].

NAME: Glutamine synthetase signamre 1.

CONSENSUS: [FYWL]-D-G-S-S-x(6,8)-[DENQSTAK]-[SA]-[DE]-x(2)-[LIVMFY].

NAME: Glutamine synthetase putanve ATP-bmding region signamre. CONSENSUS: K-P-[LIVMFYA]-x(3,5)-[NPAT]-G-[GSTAN]-G-x-H-x(3)-S.

NAME: Glutamine synthetase class-I adenylation site. CONSENSUS: K-[LIVM]-x(5)-[LIVMA]-D-[RK]-[DN]-[LI]-Y.

NAME: D-alamne-D-alamne ligase signamre 1.

CONSENSUS: H-G-x(2)-G-E-D-G-x-[LIVMA]-[QSA]-[GSA].

NAME: D-alamne-D-alamne ligase signature 2.

CONSENSUS: [LIV]-x(3)-[GA]-x-[GSAIV]-R-[LIVCA]-D-[LIVMF](2)-x(7,9)-[LI]-x-E-

CONSENSUS: [LIVA]-N-[STP]-x-P-[GA] .

NAME: SAICAR synthetase signamre 1.

CONSENSUS: [LIVMF](2)-P-[LIVM]-E-x-[LIVM]-[LIVMCA]-R-x(3)-[TA]-G-S.

N.AME: SAICAR synthetase signamre 2.

CONSENSUS: [LIVM]-[LrVMA]-D-x-K-[LIVMFY]-E-F-G.

NAME: Folylpolyglutamate synthase signamre 1.

CONSENSUS: [LIVMFY]-x-[LιVM]-[STAG]-G-T-[NK]-G-K-x-[ST]-x(7)-[LIVM](2)-x(3)-[GSK].

NAME: Folylpolyglutamate synthase signamre 2.

CONSENSUS: [LIVMFY](2)-E-x-G-[LIVM]-[GA]-G-x(2)-D-x-[GST]-x-[LIVM](2).

NAME: Ubiquitin-activating enzyme signamre 1. CONSENSUS: K-A-C-S-G-K-F-x-P.

NAME: Ubiquitin-acnvating enzyme active site. CONSENSUS: P-[LrVM]-C-T-[LIVM]-[KRH]-x-[FT]-P.

NAME: Ubiquitin-conjugating enzymes active site.

CONSENSUS: [FYWLSP]-H-[PC]-[NH]-[LIV]-x(3,4)-G-x-[LIV]-C-[LIV]-x-[LIV].

NAME: Formate-tetrahydrofolate ligase signamre 1. CONSENSUS: G-[LιVM]-K-G-G-A-A-G-G-G-Y.

NAME: Formate-tetrahydrofolate ligase signamre 2. CONSENSUS: V-A-T-[IV]-R-A-L-K-x-[HN]-G-G.

NAME: Adenylosucc ate synthetase GTP-binding site. CONSENSUS: Q-W-G-D-E-G-K-G.

NAME: Adenylosuccmate synthetase active site. CONSENSUS: G-I-[GR]-P-x-Y-x(2)-K-x(2)-R. NAME Argininosuccinate synthase signature 1 CONSENSUS A-[FY]-S-G-G-L-D-T-S

NAME Argininosuccinate synthase signamre 2 CONSENSUS G-x-T-x-K-G-N-D-x(2)-R-F

NAME Phosphoπbosylglycinamide synthetase signamre CONSENSUS R-F-G-D-P-E-x-[QM]

NAME Carbamoyl-phosphate synthase subdomain signamre 1

CONSENSUS [FYV]-[PS]-[LIVMC]-[LrVMA]-[LIVM]-[KR]-[PSA]-[STA]-x(3)-[SG]-G-x-[AG]

NAME Carbamoyl-phosphate synthase subdomain signamre 2

CONSENSUS [LIVMF]-[LIMN]-E-[LIVMCA]-N-[PATLIVM]-[KR]-[LIVMSTAC]

NAME ATP-dependent DNA ligase AMP-bmding site CONSENSUS [EDQH]-x-K-x-[DN]-G-x-R-[GACIVM]

NAME ATP-dependent DNA ligase signamre 2

CONSENSUS E-G-[LIVMA]-[LIVM](2)-[KR]-x(5,8)-[YW]-[QNEK]-x(2,6)-[KRH]-x(3,5)-K-

CONSENSUS [LIVMFY]-K

NAME NAD-dependent DNA ligase signamre 1

CONSENSUS K-[LIVM]-D-G-[LIVM]-[SA]-x(4)-Y-x(2)-G-x-L-x(4)-[ST]-R-G-[DN]-G-x(2)-G-

CONSENSUS [DEHDENL]

NAME NAD-dependent DNA ligase signamre 2

CONSENSUS [IV]-G-[KR]-[ST]-G-x-[LIVM] [STNK]-x-[VT]-x(2)-L-x-[PS]-V

NAME RNA 3' -terminal phosphate cyclase signamre CONSENSUS [RH]-G-x(2)-P-x G(3)-x-[LIV]

NAME Lipoate-protein ligase B signamre

CONSENSUS R-G-G-X(2)-T-[FYW]-H-X(2)-[GH]-Q-X-[LΓV]-X-Y

NAME Isopenicillin N synthetase signamre 1 CONSENSUS [RK]-x-[STA]-x(2)-S-x-C-Y-[SL]

NAME Isopenicillin N synthetase signamre 2

CONSENSUS [LIVM](2)-x-C-G-[STA]-x(2)-[STAG]-x(2)-T-x-[DNG]

NAME Site-specific recombinases active site CONSENSUS Y-[LIVAC]-R-[VA]-S-[ST]-x(2)-Q

NAME Site-specific recombinases signamre 2

CONSENSUS G-[DE]-x(2)-[LIVM]-x(3)-[LIVM]-[DT]-R-[LIVM]-[GSA]

NAME Transposases, Mutator family, signamre

CONSENSUS D-x(3)-G-rLrVMF]-x(6)-[STAV]-[LIVMFYW]-[PT]-x-[STAV]-x(2)-[QR]-x-C-x(2)-

CONSENSUS H

NAME Transposases, IS30 family, signamre

CONSENSUS R-G-x(2)-E-N-x-N-G-[LIVM](2)-R-[QE]-[LIVMFY](2)-P-K

NAME Autoinducers synthetases family signamre

CONSENSUS [LMFY]-R-x(3)-F-x(2)-[KR]-x(2)-W-x-[LIVM]-x(6,9)-E-x-D-x-[FY]-D

NAME Thiamine pyrophosphate enzymes signamre

CONSENSUS [LrVMF]-[GSA]-x(5)-P-x(4)-[LIVMFYW]-x-[LIVMF]-x-G-D-[GSA]-[GSAC]

NAME Biotin-requiπng enzymes attachment site

CONSENSUS [GN]-[DEQTR]-x-[LIVMFY]-x(2)-[LIVM]-x-[AIV]-M-K-[LMAT]-x(3)-[LIVM]-x-

CONSENSUS [SAV]

NAME 2-oxo acid dehydrogenases acyltransferase component lipoyl bmding site

CONSENSUS [GN]-x(2)-[LIVF]-x(5)-[LIVFC]-x(2)-[LIVFA]-x(3)-K-[STAIV]-[STAVQDN]-

CONSENSUS x(2)-[LIVMFS]-x(5)-[GCN]-x-[LIVMFY]

NAME Putative AMP-binding domain signamre

CONSENSUS [LIVMFY]-x(2)-[STG]-[STAG]-G-[ST]-[STEI]-[SG]-x-[PASLIVM]-[KR]

NAME Molybdenum cofactor biosynthesis proteins signamre 1 CONSENSUS [LIVM](3)-[LIT](2)-G-G-T-G-x(4)-D NAME: Molybdenum cofactor biosynthesis proteins signamre 2.

CONSENSUS S-x-[GS]-x(2)-D-x(5)-[LIVW]-x( 10, 12)-[LIV]-X(2)-[KR]-P-G-[KRL]-P-X(2)-

CONSENSUS- [LΓVMF]-[GA].

NAME: moaA / nifB / pqqE family signamre.

CONSENSUS: [LIV]-x(3)-C-[NP]-[LIVMF]-[QRS]-C-x-[FYM]-C.

NAME. Radical activating enzymes signamre.

CONSENSUS [GV]-x-G-x-[KR]-x(3)-F-x(2)-G-x(0,l)-C-x(3)-C-x(2)-C-x-[NL]

NAME Tpx family signamre.

CONSENSUS: S-x-D-L-P-F-A-x(2)-[KR]-[FW]-C.

NAME- Cytochrome c family heme-binding site signamre. CONSENSUS: C-{CPWHF}-{CPWR}-C-H-{CFYW}.

NAME: Cytochrome b5 family, heme-binding domain signamre. CONSENSUS [FY]-[LIVMK]-x(2)-H-P-[GA]-G

NAME: Cytochrome b/b6 heme- gand signature

CONSENSUS. [DENQ]-x(3)-G-[FYWMQ]-x-[LIVMF]-R-x(2)-H.

NAME: Cytochrome b/b6 Qo site signamre. CONSENSUS: P-[DE]-W-[FY]-[LFY](2)

NAME: Cytochrome b559 subunits heme-binding site signamre.

CONSENSUS: [LIV]-x-[ST]-[LIVF]-R-[FYW]-x(2)-[IV]-H-[STGA]-[LIV]-[STGA]-[IV]-P.

NAME. Nickel-dependent hydrogenases b-type cytochrome subumt signamre 1

CONSENSUS R-[LIVMFYW]-X-H-W-[LΓVM]-X(2)-[LIVMF]-[STAC]-[LIVM]-X(2)-L-X-[LIVM]-T-G

NAME- Nickel-dependent hydrogenases b-type cytochrome subunit signamre 2

CONSENSUS- [RH]-[STA]-[LIVMFYW]-H-[RH]-[LΓVM]-X(2)-W-X-[LIVMF]-X(2)-F-X(3)-H.

NAME Succinate dehydrogenase cytochrome b subumt signamre 1

CONSENSUS. R-P-[LΓVMT]-X(3)-[LIVM]-X(6)-[LIVMWPK]-X(4)-S-X(2)-H-R-X-[ST]

NAME- Succinate dehydrogenase cytochrome b subumt signamre 2

CONSENSUS H-X(3)-[GA]-[LIVMT]-R-[HF]-[LIVMF]-X-[FYWM]-D-X-[GVA].

NAME: Thioredoxin family acnve site

CONSENSUS- [LIVMF]-[LIVMSTA]-X-[LIVMFYC]-[FYWSTHE]-X(2)-[FYWGTN]-C-[GATPLVE]-

CONSENSUS [PHYWSTA]-C-x(6)-[LIVMFYWT] .

NAME- Glutaredoxin active site.

CONSENSUS: [LIVD]-[FYSA]-x(4)-C-[PV]-[FYW]-C-x(2)-[TAV]-x(2,3)-[LIV].

NAME: Type-1 copper (blue) proteins signamre

CONSENSUS. [GA]-x(0,2)-[YSA]-x(0,l)-[VFY]-x-C-x(l,2)-[PG]-x(0,l)-H-x(2,4)-[MQ].

NAME: 2Fe-2S ferredoxins, iron-sulfur bmding region signamre. CONSENSUS C-{C}-{C}-[GA]-{C}-C-[GAST]-{CPDEKRHFYW}-C.

NAME: Adrenodoxin family, iron-sulfur binding region signamre. CONSENSUS: C-x(2)-[STAQ]-x-[STAMV]-C-[STA]-T-C-[HR].

NAME: 4Fe-4S ferredoxins, iron-sulfur binding region signamre. CONSENSUS: C-x(2)-C-x(2)-C-x(3)-C-[PEG].

NAME: High potential iron-sulfur proteins signamre CONSENSUS: C-x(6,9)-[LIVM]-x(3)-G-[YW]-C-x(2)-[FYW].

NAME: Rieske iron-sulfur protein signamre 1. CONSENSUS. C-[TK]-H-L-G-C-[LIVT].

NAME: Rieske iron-sulfur protein signamre 2. CONSENSUS: C-P-C-H-x-[GSA]

NAME: Flavodoxin signamre.

CONSENSUS: [LIV]-[LIVFY]-[FY]-x-[ST]-x(2)-[AGC]-x-T-x(3)-A-x(2)-[LIV].

NAME: Rubredoxm signature

CONSENSUS: [LIVM]-x(3)-W-x-C-P-x-C-[AGD]. NAME Electron transfer flavoprotein alpha-subumt signamre

CONSENSUS [LI]-Y-[LIVM]-[AT]-x-G-[IV]-[SD]-G-x-[IV]-Q-H-x(2)-G-x(6)-[IV]-x-A-

CONSENSUS [IV]-N

NAME Electron transfer flavoprotein beta-subumt signamre

CONSENSUS [IVA]-x-[KR]-x(2)-[DE]-[GD]-[GDE]-x( 1 ,2)-[EQ]-x-[LIV]-x(4)-P-x-[LIVM](2)-

CONSENSUS [TAC]

NAME Vertebrate metallothioneins signamre

CONSENSUS C-x-C-[GSTAP]-x(2)-C-x-C-x(2)-C-x-C-x(2)-C-x-K

NAME Ferπtin iron-binding regions signamre 1

CONSENSUS E-x-[KR]-E-x(2)-E-[KR]-[LF]-[LIVMA]-x(2)-Q-N-x-R-x-G-R

NAME Ferπtin iron-binding regions signamre 2

CONSENSUS D-x(2)-[LIVMF]-[STAC]-[DH]-F-[LI]-[EN]-x(2)-[FY]-L-x(6)-[LIVM]-[KN]

NAME Bacteπoferπtm signature

CONSENSUS < M-x-G-x(3)-V-[LIV]-x(2)-[LM]-x(3)-L-x(3)-L

NAME Transfernns signature 1

CONSENSUS Y-x(0, 1)-[VAS]-V-[IVAC]-[IVA]-[IVA]-[RKH]-[RKS]-[GDENSA]

NAME Transfernns signamre 2

CONSENSUS Y-x-G-A-[FL]-[KRHNQ]-C-L-x(3,4)-G-[DENQ]-V-[GA]-[FYW]

NAME Transfernns signamre 3

CONSENSUS [DENQ]-[YF]-x-[LY]-L-C-x-[DN]-x(5,8)-[LIV]-x(4,5)-C-x(2)-A-x(4)-[HQR]-x-

CONSENSUS [LIVMFYWHLIVM]

NAME Globins profile

NAME Protozoan cyanobactenal globins signamre

CONSENSUS F-[LF]-x(5)-G-[PA]-x(4)-G-[KRA]-x-[LIVM]-x(3)-H

NAME Plant hemoglobins signamre CONSENSUS [SN]-P-x-L-x(2)-H-A-x(3)-F

NAME Hemerythnns signamre CONSENSUS W-L-x-[NQ]-H-I-x(3)-D-F

NAME Arthropod hemocyanins / insect LSPs signamre 1 CONSENSUS Y [FYW]-x-E-D-[LIVM]-x(2)-N-x(6)-H-x(3)-P

NAME Arthropod hemocyanins / insect LSPs signamre 2 CONSENSUS T-x(2)-R-D-P-x-[FY]-[FYW]

NAME Heavy-metal-associated domain

CONSENSUS [LIVN]-x(2)-[LIVMFA]-x-C-x-[STAGCDNH]-C-x(3)-[LIVFG]-x(3)-[LIV]-x(9, 11 )-

CONSENSUS [IVA]-x-[LVFYS]

NAME ABC transporters family signamre

CONSENSUS [LIVMFYC]-[SA]-[SAPGLVFYKQH]-G-[DENQMW]-[KRQASPCLIMFW]-[KRNQSTAVM]-

CONSENSUS [KRACLVM]-[LΓVMFYPAN]-{PHY}-[LΓVMFW]-[SAGCLIVP]-{FYWHP}-{KRHP}-

CONSENSUS [LIVMFYWSTA]

NAME Binding-protein-dependent transport systems inner membrane comp sign

CONSENSUS [LIVMFY]-x(8)-[EQR]-[STAGV]-[STAG]-x(3)-G-[LIVMFYSTAC]-x(5)-[LIVMFYSTA]-

CONSENSUS x(4)-[LIVMFY]-[PKR]

NAME ABC-2 type transport system integral membrane proteins signamre

CONSENSUS [LIMST]-x(2)-[L]MW]-x(2)-[LIMCA]-[GSTC]-x-[GSAIV]-x(6)-[LIMGA]-[PGSNQ]-

CONSENSUS x(9, 12)-P-[LIMFT]-x-[HRSY]-x(5)-[RQ]

NAME Bacterial extracellular solute-binding proteins, family 1 signamre

CONSENSUS [GAP]-[LIVMFA]-[STAVDN]-x(4)-[GSAV]-[LIVMFY](2)-Y-[ND]-x(3)-[LIVMF]-x-

CONSENSUS [KNDE]

NAME Bacterial extracellular solute-binding proterns, family 3 signamre

CONSENSUS G-[FYIL]-[DE]-[LIVMT]-[DE]-[LIVMF]-x(3)-[LIVMA]-rVAGC]-x(2)-[LrVMAGN]

NAME Bacterial extracellular solute-binding proterns, family 5 signamre

CONSENSUS [AG]-X(6,7)-[DNEG]-X(2)-[STAVE]-[LIVMFYWA]-X-[LIVMFY]-X-[LΓVM]-[KR]- CONSENSUS [KRHDE]-[GDN]-[LIVMA]-[KNGSP]-[FW]

NAME Serum albumin family signamre

CONSENSUS [FY]-x(6)-C-C-x(7)-C-[LFY]-x(6)-[LIVMFYW]

NAME Transthyretin signamre 1

CONSENSUS S-K-C-P-L-M-V-K-V-L-D-[AS]-V-R-G

NAME Transthyretin signamre 2

CONSENSUS S-P-[FY]-S-[FY]-S-T-T-A-[LIVM]-V-[ST]-x-P

NAME Avidin / Streptavidin family signamre

CONSENSUS [DEN]-x(2)-[KR]-[STA]-x(2)-V-G-x-[DN]-x-[F ]-T-[KR]

NAME Eukaryotic cobalamin-binding proteins signamre CONSENSUS [SN]-V-D-T-[GA]-A-[LIVM]-A-x-L-A-[LIVMF]-T-C

NAME Lipocahn signamre

CONSENSUS [DENG]-x-[DENQGSTARK]-x(0,2)-[DENQARK]-[LIVFY]-{CP}-G-{C}-W-[FYWLRH]-x-

CONSENSUS [LIVMTA]

NAME Cytosolic fatty-acid binding proteins signamre

CONSENSUS [GSAIVK]-x-[FYW]-x-[LIVMF]-x(4)-[NHG]-[FY]-[DE]-x-[LIVMFY]-[LIVM]-x(2)-

CONSENSUS [LIVMAKR]

NAME Acyl-CoA-binding protein signamre

CONSENSUS P-[STA]-x-[DEN]-x-[LIVMF]-x(2)-[LIVMFY]-Y-[GSTA]-x-[FY]-K-Q-[STA](2)-x-G

NAME LBP / BPI / CETP family signamre

CONSENSUS [PA]-[GA]-[LΓVMC]-X(2)-R-[IV]-[ST]-X(3)-L-X(5)-[EQ]-X(4)-[LIVM]-[EQK]-

CONSENSUS x(8)-P

NAME Phosphatidylethanolamiπe-binding protein family signamre CONSENSUS [FY]-x-[LIVMF](3)-x-[DC]-P-D-x-P-[SN]-x( 10)-H

NAME Plant lipid transfer proteins signamre

CONSENSUS [LIVM]-[PA]-x(2)-C-x-[LIVM]-x-[LIVM]-x-[LIVMFY]-x-[LIVM]-[ST]-x(3)-

CONSENSUS [DN]-C-x(2)-[LIVM]

NAME Uteroglobin family signamre 1

CONSENSUS [GA]-x(3)-I C-P-x-[LIVMF]-x(3)-[LIVM]-[DE]-x-[LIVMF](2)

NAME Uteroglobin family signamre 2

CONSENSUS [DEQ]-x(4)-[SN]-x(5)-[DEQ]-x-I-x(2) S-[PSE]-[LS]-C

NAME Mitochondrial energy transfer proteins signamre

CONSENSUS P-x-[DE]-x-[LIVAT]-[RK]-x-[LRH]-[LIVMFY]-[QMAIGV]

NAME Sugar transport proteins signamre 1

CONSENSUS [LrVMSTAG]-[LIVMFSAG]-x(2)-[LIVMSA]-[DE]-x-[LIVMFYWA]-G-R-[RK]-x(4,6)-

CONSENSUS [GSTA]

NAME Sugar transport proteins signamre 2

CONSENSUS [LIVMF]-x-G-[LIVMFA]-x(2)-G-x(8)-[LIFY]-x(2)-[EQ]-x(6)-[RK]

NAME LacY family proton/sugar symporters signamre 1

CONSENSUS G-[LIVM](2)-x-D-[RK]-L-G-L-[RK](2)-x-[LIVM](2)-W

NAME LacY family proton/sugar symporters signamre 2

CONSENSUS P-x-[LIVMF](2)-N-R-[LIVM]-G-x-K-N-[STA]-[LIVM](3)

NAME PTR2 family proton/oligopeptide symporters signamre 1

CONSENSUS [GA]-[GAS]-[LIVMFYWA]-[LIVM]-[GAS]-D-X-[LIVMFYWT]-[LΓVMFYW]-G-X(3)-[TAV]-

CONSENSUS [IV]-X(3)-[GSTAV]-X-[LIVMF]-X(3)-[GA]

NAME PTR2 family proton ohgopepnde symporters signamre 2

CONSENSUS [FYT]-X(2)-[LMFY]-[FYV]-[LIVMFYWA]-X-[IVG]-N-[LIVMAG]-G-[GSA]-[LIMF]

NAME Amilonde-sensitive sodium channels signature

CONSENSUS Y-x(2)-[EQTF]-x-C-x(2)-[GSTDNL]-C-x-[QT]-x(2)-[LIVMT]-[LIVMS]-x(2)-C-x-C

NAME Sodium alamne symporter family signamre

CONSENSUS G-G-X-[GA](2)-[LΓVM]-F-W-M-W-[LΓVM]-X-[STAV]-[LIVMFA](2)-G NAME Sodium dicarboxylate symporter family signamre 1

CONSENSUS P-x(0,l)-G-[DE]-x-[LIVMF](2)-x-[LIVM](2)-[KREQ]-[LIVM](3)-x-P

NAME Sodium dicarboxylate symporter family signamre 2

CONSENSUS P-x-G-x-[STA]-x-[NT]-[LIVMC]-D-G-[STAN]-x-[LIVM]-[FY]-x(2)-[LIVM]-x(2)-

CONSENSUS [LIVM]-[FY]-[LI]-[SA]-Q

NAME Sodium galactoside symporter family signamre

CONSENSUS D-x(3)-G-x(3)-[DN]-x(6,8)-G-[KH]-F-[KR]-P-[FYW]-[LIVM](2)-x-[GSTA](2)

NAME Sodium neurotransmitter symporter family signamre 1 CONSENSUS W-R-F-[GP]-Y-x(4)-N-G-G-G-x-[FY]

NAME Sodium neurotransmitter symporter family signamre 2

CONSENSUS Y-[LIVMFY]-x(2)-[SC]-[LIVMFY]-[STQ]-x(2)-L-P-W-x(2)-C-x(4)-N-[GST]

NAME Sodium solute symporter family signamre 1

CONSENSUS [GS]-x(2)-[LIY]-x(3)-[LIVMFYWSTAG](10)-[LIY]-[TAV]-x(2)-G-G-[LMF]-x-

CONSENSUS [SAP]

NAME Sodium solute symporter family signamre 2

CONSENSUS [GAST]-[LrVM]-x(3)-[KR]-x(4)-G-A-x(2)-[GAS]-[LIVMGS]-[LIVMW]-[LIVMGAT]-G-

CONSENSUS x-[LIVMG]

NAME Sodium sulfate symporter family signamre

CONSENSUS [STACP]-S-x(2)-F-x(2)-P-[LIVM]-[GSA]-x(3)-N-x-[LIVM]-V glpT family of transporters signamre CONSENSUS R-G-x(5)-W-N-x(2)-H-N-x-G-G

NAME Ammonium transporters signamre

CONSENSUS D-[FYWS]-A-G-[GSC]-x(2)-[IV]-x(3)-[SAG](2)-x(2)-[SAG]-[LIVMF]-x(3)-

CONSENSUS [LIVMFYWA](2)-x-[GK]-x-R

NAME BCCT family of transporters signamre CONSENSUS [GSDN]-W-T-[LIVM]-x-[FY]-W-x-W-

NAME Flagellar motor protein motA family signamre

CONSENSUS A-[LMF]-x-[GAT]-T-[LIVF]-x-G-x-[LIVMF]-x(7)-P

NAME Formate and mtnte transporters signamre 1

CONSENSUS [LIVMA]-[LIVMY]-x-G-[GSTA]-[DES]-L-[FI]-[TN]-[GS]

NAME Formate and mtnte transporters signamre 2 CONSENSUS [GA]-x(2)-[CA]-N [LIVMFYW](2)-V-C-[LV]-A

NAME Prokaryotic sulfate-binding proteins signamre 1 CONSENSUS K-x-[NQEK]-[GT]-G-[DQ]-x-[LIVM]-x(3)-Q-S

NAME Prokaryonc sulfate-binding proteins signamre 2 CONSENSUS N-P-K-[ST]-S-G-x-A-R

NAME Sulfate transporters signamre

CONSENSUS P-x-Y-[GS]-L-Y-[STAG](2)-x(4)-[LIVMFY](3)-x(3)-[GSTA](2)-S-[KR]

NAME Amino acid permeases signamre

CONSENSUS [STAGC]-G-[PAG]-x(2,3)-[LIVMFYWA](2)-x-[LiVMFYW]-x-[LrVMFWSTAGC](2)-

CONSENSUS [STAGC]-x(3)-[LIVMFYW]-x-[LIVMST]-x(3)-[LIMCTA]-[GA]-E-x(5)-[PSAL]

NAME Aromatic ammo acids permeases signamre

CONSENSUS I-G-[GA]-G-M-[LF]-[SA]-x-P-x(3)-[SA]-G-x(2)-F

NAME Xanthine/uracil permeases family signamre

CONSENSUS [LΓVM]-P-X-[PASIF]-V-ΓLIVM]-G-G-X(4)-[LIVM]-[FY]-[GSA]-X-[LIVM]-X(3)-G

NAME Amon exchangers family signamre 1

CONSENSUS F-G-G-[LIVM](2)-[KR]-D-[LIVM]-[RK]-R-R-Y

NAME Amon exchangers family signamre 2 CONSENSUS [FTJ-L-I-S-L-I-F-I-Y-E-T-F-x-K-L

NAME MIP family signamre

CONSENSUS [HNQA]-x-N-P-[STA]-[LIVMF]-[ST]-[LIVMF]-[GSTAFY] NAME General diffusion Gram-negative poπns signamre

CONSENSUS: [LIVMFY]-x(2)-G-x(2)-Y-x-F-x-K-x(2)-[SN]-[STAV]-[LIVMFYW]-V

NAME: OmpA-hke domain.

CONSENSUS [LIVMA]-x-[GT]-x-[TA]-[DA]-x(2)-[DG]-[GSTP]-x(2)-[LFYDE]-[NQS]-x(2)-

CONSENSUS. [LI]-[SG]-[QE]-[KRQE]-R-A-x(2)-[LV]-x(3)-[LIVMF]-x(4,5)-[LIVM]-x(4)-

CONSENSUS- [LIVM]-x(3)-[SG]-x-G

NAME: Eukaryotic mitochondπal poπn signamre.

CONSENSUS: [YH]-x(2)-D-[SPA]-x-[STA]-x(3)-[TAG]-[KR]-[LIVMF]-[DNSTA]-[DNS]-x(4)-

CONSENSUS: [GSTAN]-[LIVMA]-x-[LIVMY]

NAME Insulin-like growth factor binding proteins signamre CONSENSUS. G-C-[GS]-C-C-x(2)-C-A-x(6)-C.

NAME: GPRl/FUN34/yaaH family signature CONSENSUS: N-P-[AV]-P-[LF]-G-L-x-[GSA]-F.

NAME: GNS1/SUR4 family signamre. CONSENSUS. L-x-F-L-H-x-Y-H-H.

NAME: 43 Kd postsynaptic protein signamre CONSENSUS- G-Q-D-Q-T-K-Q-Q-I.

NAME Actins signamre 1

CONSENSUS: [FY]-[LIV]-G-[DE]-E-A-Q-x-[RKQ](2)-G.

NAME Actins signamre 2

CONSENSUS: W-[IV]-[STA]-[RK]-x-[DE]-Y-[DNE]-[DE] .

NAME Actins and actm-related proterns signamre

CONSENSUS: [LM]-[LIVM]-T-E-[GAPQ]-x-[LIVMFYWHQ]-N-[PSTAQ]-x(2)-N-[KR].

NAME Annexins repeated domain signamre.

CONSENSUS [TG]-[STV]-x(8)-[LIVMF]-x(2)-R-x(3)-[DEQNH]-x(7)-[IFY]-x(7)-[LIVMF]-

CONSENSUS x(3)-[LIVMF]-x(l l)-[LIVMFA]-x(2)-[LIVMF].

NAME. Caveolins signamre. CONSENSUS- F-E-D-V-I-A-E-P

NAME: Clathrm light chain signamre 1. CONSENSUS: F-L-A-Q-Q-E-S

NAME- Clathrm light chain signamre 2.

CONSENSUS: [KR]-D-x-S-[KR]-[LIVM]-[KR]-x-[LIVM](3)-x-L-K

NAME: Clusteπn signamre 1 CONSENSUS: C-K-P-C-L-K-x-T-C

NAME: Clusteπn signamre 2.

CONSENSUS: C-L-[RK]-M-[RK]-x-[EQ]-C-[ED]-K-C.

NAME: Connexins signamre 1.

CONSENSUS. C-[DN]-T-x-Q-P-G-C-x(2)-V-C-Y-D

NAME: Connexins signamre 2.

CONSENSUS: C-x(3,4)-P-C-x(3)-[LIVM]-[DEN]-C-[FY]-[LIVM]-[SA]-[KR]-P.

NAME. Crystallins beta and gamma 'Greek key' motif signamre.

CONSENSUS: [LrVMFYWA]-x-{DEHRKSTP}-[FY]-[DEQHKY]-x(3)-[FY]-x-G-x(4)-[LrVMFCST].

NAME: Dynamin family signamre

CONSENSUS: L-P-[RK]-G-[STN]-[GN]-[LIVM]-V-T-R.

NAME: Dynein light chain type 1 signamre.

CONSENSUS. H-x-I-x-G-[KR]-x-F-[GA]-S-x-V-[ST]-[HY]-E.

NAME: FtsZ protein signamre 1.

CONSENSUS: N-[ST]-D-x-Q-x-L-x(16,18)-G-x-G-[ATV]-G-[GSAN]-x-P-x(2)-G.

NAME: FtsZ protein signamre 2.

CONSENSUS [DNHKR]-[LΓVMF]-X-[LΓVMF](2)-[VSTAC]-[STAC]-G-X-G-[GK]-G-T-G-[ST]-G-

CONSENSUS: [GSAR]-[STA]-P-[LIVMFT]-[LIVMF]-[SGAV] NAME Fungal hydrophobins signamre

CONSENSUS [GN]-[DNQPSA]-X-C-[GSTANK]-[GSTADNQ]-[STNQI]-[PTΓV]-X-C-C-[DENQKPST]

NAME Intermediate filaments signamre

CONSENSUS [IV]-x-[TACI]-Y-[RKH]-x-[LM]-L-[DE]

NAME Involucrin signamre

CONSENSUS < M-S-[QH]-Q-x-T-[LV]-P-V-T-[LV]

NAME Kinesin motor domain signamre

CONSENSUS [GSA]-[KRHPSTQVM]-[LIVMF]-x-[LIVMF]-[IVC]-D-L-[AH]-G-[SAN]-E

NAME Kinesin motor domain profile

NAME Kinesin light chain repeat

CONSENSUS [DEQR]-A-L-x(3)-[GEQ]-x(3)-G-x-[DNS]-x-P-x-V-A-x(3)-N-x-L-[AS]-

CONSENSUS x(5)-[QR]-x-[KR]-[FY]-x(2)-[AV]-x(4)-[HKNQ]

NAME Myelin basic protein signamre CONSENSUS V-V-H-F-F-K-N

NAME Myelin PO protein signamre

CONSENSUS S-[KR]-S-x-K-[AG]-x-[SA]-E-K-K-[STA]-K

NAME Myelin proteo pid protein signamre 1 CONSENSUS G-[MV]-A-L-F-C-G-C-G-H

NAME Myelin proteohpid protein signamre 2

CONSENSUS C-x-[ST]-x-[DE]-x(3)-[ST]-[FY]-x-L-[FY]-I-x(4)-G-A

NAME Neuromoduhn (GAP-43) signamre 1 CONSENSUS <M-L-C-C-[LIVM]-R-R

NAME Neuromodulin (GAP-43) signamre 2 CONSENSUS S-F-R-G-H-I-x-R-K-K-[LIVM]

NAME Osteopontin signature

CONSENSUS [KQ]-x-[TA]-x(2)-[GA]-S-S-E-E-K

NAME Penpheπn / rom-1 signamre

CONSENSUS D-[GS]-V-P-F-[ST]-C-C-N-P-x-S-P-R-P-C

NAME Profilin signamre

CONSENSUS < x(0,l) [STA]-x(0,l)-W-[DENQH]-x-[YI]-x-[DEQ]

NAME Surfactant associated polypeptide SP-C palmitoylation sites CONSENSUS I-P-C-C-P-V

NAME Synapsins signamre 1 CONSENSUS L-R-R-R-L-S-D-S

NAME Synapsins signamre 2 CONSENSUS G-H-A-H-S-G-M-G-K-V-K

NAME Synaptobrevm signamre

CONSENSUS N-[LIVM]-[DENS]-[KL]-V-x-[DEQ]-R-x(2)-[KR]-[LIVM]-[STDE]-x-[LIVM]-x-[DE]-

CONSENSUS [KR]-[TA]-[DE]

NAME Synaptophysin / synaptoporm signamre CONSENSUS L-S-V-[DE]-C-x-N-K-T

NAME Tropomyosins signature CONSENSUS L-K-E-A-E-x-R-A-E

NAME Tubulin subunits alpha, beta, and gamma signamre CONSENSUS [SAG]-G-G-T-G-[SA]-G

NAME Tubulin-beta mRNA autoregulation signal CONSENSUS <M-R-[DE]-[IL]

NAME Tau and MAP proteins tubulin-binding domain signamre CONSENSUS G-S-x(2)-N-x(2)-H-x-[PA]-[AG]-G(2)

NAME Neuraxin and MAP1B proteins repeated region signamre CONSENSUS [STAGDN]-Y-x-Y-E-x(2)-[DE]-[KR]-[STAGCI]

NAME F-actin capping protein alpha subunit signamre 1 CONSENSUS V-H-[FY](2)-E-D-G-N-V

NAME F-actin capping protein alpha subunit signature 2 CONSENSUS F-K-[AE]-L-R-R-x-L-P

NAME F-actin capping protein beta subumt signature CONSENSUS C-D-Y-N-R-D

NAME Vincuhn family talin-binding region signamre

CONSENSUS [KR]-x-[LIVMF]-x(3)-[LIVMA]-x(2)-[LIVM]-x(6)-R-Q-Q-E-L

NAME Vincuhn repeated domain signamre CONSENSUS [LIVM]-x-[QA]-A-x(2)-W-[IL]-x-[DN]-P

NAME Amyloidogenic glycoprotein extracellular domain signamre CONSENSUS G-[VT]-E-[FY]-V-C-C-P

NAME Amyloidogenic glycoprotein intracellular domain signamre CONSENSUS G-Y-E-N-P-T-Y-[KR]

NAME Cadheπns extracellular repeated domain signamre CONSENSUS [LIV]-x-[LrV]-x-D-x-N-D-[NH]-x-P

NAME Insect cuticle proteins signamre

CONSENSUS G-x(7)-[DEN]-G-x(6)-Y-x-A-[DNG]-x(2,3)-G-[FY]-x-[AP]

NAME Gas vesicles protein GVPa signamre 1

CONSENSUS [LIVM]-x-[DE]-[LIVMFYT]-[LIVM]-[DE]-x-[LIVM](2)-[DKR](2)-G-x-[LIVM](2)

NAME Gas vesicles protein GVPa signamre 2

CONSENSUS R-[LIVA](3)-A-[GS]-[LΓVMFY]-X-T-X(3)-Y-[AG]

NAME Gas vesicles protein GVPc repeated domam signamre CONSENSUS F-L-x(2)-T-x(3)-R-x(3)-A-x(2)-Q-x(3)-L-x(2)-F

NAME Bacterial microcompartiments proteins signamre

CONSENSUS D-x(0, 1 )-M-x-K-[SAG](2)-x-[IV]-x-[LIVM]-[LrVMA]-[GCS]-x(4)-[GD]-[SGPD]-

CONSENSUS [GA]

NAME Flagella basal body rod proteins signamre

CONSENSUS [GTARYQ]-x(9)-[LIVMYSTA](2)-[GSTA]-[STADEN]-N-[LIVM]-[SAN]-N-x-[SADNFR]-

CONSENSUS [STV]

NAME Flagella transport protein fliP family signamre 1

CONSENSUS [PA]-A-[FY]-x-[LIVT]-[STH]-[EQ]-[LI]-x(2)-[GA]-F-[KREQ]-[IM]-G-[LIF]

NAME Flagella transport protein fliP family signamre 2

CONSENSUS P-[LIVMF]-K-[LIVMF](5)-x-[LIVMA]-[DNGS]-G-W

NAME Plant viruses icosahedral capsid proteins 'S' region signamre

CONSENSUS [FYW]-x-[PSTA]-x(7)-G-x-[LIVM]-x-[LIVM]-x-[FYWI]-x(2)-D-x(5)-P

NAME Potexviruses and carlaviruses coat protem signamre

CONSENSUS [RK]-[FYW]-A-[GAP]-F-D-x-F-x(2)-[LV]-x(3)-[GAST](2)

NAME Neurotransmitter-gated ion-channels signamre

CONSENSUS C-x-[LIVMFQ]-x-[LIVMF]-x(2)-[FY]-P-x-D-x(3)-C

NAME ATP P2X receptors signamre

CONSENSUS G-G-x-[LIVM]-G-[LrVM]-x-rrV]-x-W-x-C-[DN]-L-D-x(5)-C-x-P-x-Y-x-F

NAME G-protein coupled receptors signamre

CONSENSUS [GSTALIVMFYWC]-[GSTANCPDE]-{EDPKRH}-X(2)-ΓLΓVMNQGA]-X(2)-[LIVMFT]-

CONSENSUS [GSTANC]-[LΓVMFYWSTAC]-[DENH]-R-[FYWCSH]-X(2)-[LIVM]

NAME G-protem coupled receptors family 2 signamre 1

CONSENSUS C-X(3)-[FYWLIV]-D-X(3,4)-C-[FW]-X(2)-[STAGV]-X(8,9)-C-[PF]

NAME G-protein coupled receptors family 2 signamre 2

CONSENSUS Q-G-[LMFCA]-[LrVMFT]-[LrV]-x-[LrVFST]-[LIF]-ryFYH]-C-[LFY]-x-N-x(2)-V NAME G-protein coupled receptors family 3 signamre 1

CONSENSUS [LV]-x-N-[LIVM](2)-x-L-F-x-I-[PA]-Q-[LIVM]-[STA]-x-[STA](3)-[STAN]

NAME G-protein coupled receptors family 3 signamre 2

CONSENSUS C-C-[FYW]-x-C-x(2)-C-x(4)-[FYW]-x(2,4)-[DN]-x(2)-[STAH]-C-x(2)-C

NAME G-protein coupled receptors family 3 signamre 3 CONSENSUS F-N-E-[STA]-K-x-I-[STAG]-F-[ST]-M

NAME Visual pigments (opsins) retinal binding site

CONSENSUS [LIVMWAC]-[PGAC]-x(3)-[SAC]-K-[STALIMR]-[GSACPNV]-[STACP]-x(2)-[DENF]-

CONSENSUS [AP]-x(2)-[IY]

NAME Bacterial rhodopsins signamre 1

CONSENSUS R-Y-x-[DT]-W-x-[LIVMF]-[ST]-T-P-[LrVM](3)

NAME Bacterial rhodopsins retinal binding site

CONSENSUS [FYIV]-x-[FYVG]-[LIVM]-D-[LIVMF]-x-[STA]-K-x(2)-[FY]

NAME Receptor tyrosine kinase class II signamre CONSENSUS [DN]-[LIV]-Y-x(3)-Y-Y-R

NAME Receptor tyrosine kinase class III signamre CONSENSUS G-x-H-x-N-[LIVM]-V-N-L-L-G-A-C-T

NAME Receptor tyrosine kinase class V signamre 1

CONSENSUS F-X-[DN]-X-[GAW]-[GA]-C-[LΓVM]-[SA]-[LIVM](2)-[SA]-[LV]-[KRHQ]-[LIVA]-

CONSENSUS x(3)-[KR]-C-[PSAW]

NAME Receptor tyrosine kinase class V signamre 2

CONSENSUS C-x(2)-[DE]-G-[DEQ]-W-x(2,3)-[PAQ]-[LIVMT]-[GT]-x-C-x-C-x(2)-G-[HFY]-

CONSENSUS [EQ]

NAME Growth factor and cytokines receptors family signature 1 CONSENSUS C-[LVFYR]-x(7,8)-[STIVDN]-C-x-W

NAME Growth factor and cytokines receptors family signamre 2 CONSENSUS [STGL]-x-W [SG]-x-W-S

NAME TNFR/NGFR family cysteine-rich region signamre

CONSENSUS C-x(4,6)-[FYH]-x(5, 10)-C-x(0,2)-C-x(2,3) C-x(7, 1 l)-C-x(4,6)-[DNEQSKP]-

CONSENSUS x(2)-C

NAME TNFR/NGFR family cysteine-πch region domain

NAME Integnns alpha chain signamre CONSENSUS [FYWS]-[RK]-x-G-F-F-x-R

NAME Integnns beta chain cysteine-rich domain signamre CONSENSUS C-x-[GNQ]-x(l ,3)-G-x-C-x-C-x(2)-C-x-C

NAME Natπuretic peptides receptors signamre CONSENSUS G-P-x-C-x-Y-x-A-A-x-V-x-R-x(3)-H-W

NAME Photosynthetic reaction center proteins signamre

CONSENSUS [NH]-x(4)-P-x-H-x(2)-[SAG]-x(ll)-[SAGC]-x-H-[SAG](2)

NAME Antenna complexes alpha subunits signamre

CONSENSUS [LIVFAG]-x-[GASV]-[LIVFA]-x-[IV]-H-x(3)-[LrVM]-[GSTAE]-[STANH]-x(l,3)-

CONSENSUS [STN]-W-[LIVMFYW]

NAME Antenna complexes beta subunits signamre

CONSENSUS [EQ]-x(4)-H-x(5)-[GSTA]-x(3)-[FY]-x(3)-[AG]-x(2)-[AV]-H-x(7)-P

NAME Photosystem I psaA and psaB proteins signamre CONSENSUS C-D-G-P-G-R-G-G-T-C

NAME Photosystem I psaG and psaK proteins signamre

CONSENSUS G-F-x-[LIVM]-x-[DEA]-x(2)-[GA]-x-[GTA]-[SA]-x-G-H-x-[LIVM]-[GA]

NAME Phytochrome chromophore attachment site signamre CONSENSUS [RGS]-[GSA]-[PV]-H-x-C-H-x(2)-Y

NAME Phytochrome chromophore attachment site domain profile NAME Speract receptor repeated domain signamre

CONSENSUS G-x(5)-G-x(2)-E-x(6)-W-G-x(2)-C-x(3)-[FYW]-x(8)-C-x(3)-G

NAME TonB-dependent receptor proteins signamre 1

CONSENSUS < x(10, 115)-[DENF]-[ST]-[LIVMF]-[LIVSTEQ]-V-x-[AGP]-[STANEQPK]

NAME TonB-dependent receptor proteins signamre 2

CONSENSUS [LYGSTANE]-x(3)-[GSTAENQ]-x-[PGE]-R-x-[LIVFYWA]-x-[LrVMFTA]-[STAGNQ]-

CONSENSUS [LIVMFYGTA]-x-[LIVMFYWGTADQ]-x-F >

NAME Transmembrane 4 family signamre

CONSENSUS G-x(3)-[LIVMF]-x(2)-[GSA]-[LIVMF](2)-G-C-x-[GA]-[STA]-x(2)-[EG]-x(2)-

CONSENSUS [CWN]-[LIVM](2)

NAME Bacterial chemotaxis sensory transducers signamre

CONSENSUS R-T-E-[EQ]-Q-x(2)-[SA]-[LIVM]-x-[EQ]-T-A-A-S-M-E-Q-L-T-A-T-V

NAME ER lumen protein retaining receptor signamre 1 CONSENSUS G-I-S-x-[KR]-x-Q-x-L-[FY]-x-[LIV](2)-F-x(2)-R-Y

NAME ER lumen protein retaining receptor signamre 2 CONSENSUS L-E-[SA]-V-A-I-[LM]-P-Q-L

NAME Ephπns signamre

CONSENSUS [KRQ]-[LF]-[CST]-x-K-[IF]-Q-x-[FY]-[ST]-[PA]-x(3)-G-x-E-F-x(5)-[FY](2)-

CONSENSUS x(2)-[SA]

NAME Granulms signamre

CONSENSUS C-x-D-x(2)-H-C-C-P-x(4)-C

NAME HBGF/FGF family signamre

CONSENSUS G-x-L-x-[STAGP]-x(6,7)-[DE]-C-x-[FM]-x-E-x(6)-Y

NAME PTN/MK hepaπn-binding protein family signamre 1

CONSENSUS S-[DE]-C-x-[DE]-W-x-W-x(2)-C-x-P-x-[SN]-x-D-C-G-[LIVMA]-G-x-R-E-G

NAME PTN/MK hepaπn-binding protein family signature 2

CONSENSUS C-[KR]-[LIVM]-P-C-N- -K-K-x-F-G-A-[DE]-C-K-Y-x-F-[EQ]-x-W-G-x-C

NAME Nerve growth factor family signamre

CONSENSUS G-C-[KR]-G-[LIV]-[DE]-x(3)-[YW]-x-S-x-C

NAME Platelet-deπved growth factor (PDGF) family signamre CONSENSUS P-[PS]-C-V-x(3)-R-C-[GSTA]-G-C-C

NAME Small cytokines (intercnne/chemokine) C-x-C subfamily signamre

CONSENSUS C-x-C-[LIVM]-x(5,6)-[LIVMFY]-x(2)-[RKSEQ]-x-[LIVM]-x(2)-[LIVM]-x(5)-

CONSENSUS [SAG]-x(2)-C-x(3)-[EQ]-[LIVM](2)-x(9, 10)-C-L-[DN]

NAME Small cytokines (intercnne/chemokine) C-C subfamily signamre

CONSENSUS C-C-[LIFYT]-x(5,6)-[LI]-x(4)-[LIVMF]-x(2)-[FYW]-x(6,8)-C-x(3,4)-[SAG]-

CONSENSUS [LIVM](2)-[FL]-x(8)-C-[STA]

NAME TGF-beta family signamre

CONSENSUS [LrVM]-x(2)-P-x(2)-[FY]-x(4)-C-x-G-x-C

NAME TNF family signamre

CONSENSUS [LV]-x-[LIVM]-x(3)-G-[LIVMF]-Y-[LIVMFY](2)-x(2)-[QEKHL]-[LIVMGT]-x-

CONSENSUS [LIVMFY]

NAME TNF family profile

NAME Wnt-1 family signamre

CONSENSUS C-K-C-H-G-[LIVMT]-S-G-x-C

NAME Interferon alpha, beta and delta family signamre

CONSENSUS [FYH]-[FY]-x-[GNRC]-[LIVM]-x(2)-[FY]-L-x(7)-[CY]-A-W

NAME Granulocyte-macrophage colony-stimulanng factor signamre CONSENSUS C-P-[LP]-T-x-E-[ST]-x-C

NAME Interleukin- 1 signamre

CONSENSUS [FC]-x-S-[ASLV]-x(2)-P-x(2)-[FYLIV]-[LI]-[SCA]-T-x(7)-[LIVM] NAME Interleukιn-2 signamre

CONSENSUS T-E-[LF]-x(2)-L-x-C-L-x(2)-E-L

NAME Interleukins -4 and -13 signamre

CONSENSUS L-x-E-[LIVM](2)-x(4,5)-[LIVM]-[TL]-x(5,7)-C-x(4)-[IVA]-x-[DNS]-[LIVMA]

NAME Interleukιn-6 / G-CSF / MGF signamre CONSENSUS C-x(9)-C-x(6)-G-L-x(2)-[FY]-x(3)-L

NAME Interleukm-7 and -9 signamre CONSENSUS N-x-[LAP]-[SCT]-F-L-K-x-L-L

NAME Interleukin- 10 family signamre

CONSENSUS [GS]-C-x(2)-[LV]-x(2)-[LIVM](2)-x-F-Y-L-x(2)-V

NAME LIF / OSM family signamre

CONSENSUS [PST]-x(4)-F-[NQ]-x-K-x(3)-C-x-[LF]-L-x(2)-Y-[HK]

NAME Macrophage migration inhibitory factor family signamre CONSENSUS [DE]-P-C-A-x(3)-[LIVM]-x-S-I-G-x-[LIVM]-G

NAME Adipokinetic hormone family signamre CONSENSUS Q-[LV]-[NT]-[FY]-[ST]-x(2)-W

NAME Bombesin-like peptides family signamre CONSENSUS W-A-x-G-[SH]-[LF]-M

NAME Calcitomn / CGRP / IAPP family signamre

CONSENSUS C-[SAGDN]-[STN]-x(0, 1 )-[SA]-T-C-[VMA]-x(3)-[LYF]-x(3)-[LYF]

NAME Corticotropin-releasing factor family signamre

CONSENSUS [PQ]-x-[LIVM]-S-[LIVM]-x(2)-[PST]-[LIVMF]-x-[LIVM]-L-R-x(2)-[LIVM]

NAME Crustacean CHH/MIH/GIH neurohoπnones family signamre CONSENSUS C-[DENK]-D-C-x N-[LIV]-[FY]-R-x(7)-C-[KR]-x(2)-C

NAME Erythropoietin / thrombopoeitin signamre CONSENSUS P-x(4)-C-D-x-R-[LIVM](2)-x-[KR]-x(14)-C

NAME Granins signamre 1

CONSENSUS [DE]-[SN]-L-[SAN]-x(2)-[DE]-x-E-L

NAME Gramns signamre 2

CONSENSUS C-[LIVM](2)-E-[LIVM](2)-S-[DN]-[STA]-L-x-K-x-S-x(3)-[LIVM]-[STA]-x-E-C

NAME Galamn signamre

CONSENSUS G-W-T-L-N-S-A-G-Y-L-L-G-P-H

NAME Gastnn / cholecystokimn family signamre CONSENSUS Y-x(0,l)-[GD]-[WH]-M-[DR]-F

NAME Glucagon / GIP / secretin / VIP family signamre

CONSENSUS [YH]-[STAΓVGD]-[DEQ]-[AGF]-[LIVMSTE]-[FYLR]-X-[DENSTAK]-[DENSTA]-

CONSENSUS [LIVMFYG]-X(9)-[KREQL]-[KRDENQL]-[LVFYWG]-[LIVQ]

NAME Glycoprotein hormones alpha chain signamre 1 CONSENSUS C-x-G-C-C-[FY]-S-R-A-[FY]-P-T-P

NAME Glycoprotem hormones alpha chain signamre 2 CONSENSUS N-H-T-x-C-x-C-x-T-C-x(2)-H-K

NAME Glycoprotem hormones beta chain signamre 1 CONSENSUS C-[STAGM]-G-[HFYL]-C-x-[ST]

NAME Glycoprotein hormones beta chain signamre 2

CONSENSUS [PA]-V-A-x(2)-C-x-C-x(2)-C-x(4)-[STD]-[DEY]-C-x(6,8)-[PGSTAVM]-x(2)-C

NAME Gonadotropin-releasing hormones signamre CONSENSUS Q-H-[FYW]-S-x(4)-P-G

NAME Insulin family signamre

CONSENSUS C-C-{P}-x(2)-C-[STDNEKPI]-x(3)-[LIVMFS]-x(3)-C NAME Natπuretic peptides signamre. CONSENSUS C-F-G-x(3)-D-R-I-x(3)-S-x(2)-G-C

NAME Neurohypophysial hormones signamre. CONSENSUS: C-[LIFY](2)-x-N-[CS]-P-x-G.

NAME Neuromedin U signamre CONSENSUS: F-[LIVMF]-F-R-P-R-N

NAME: Endogenous opioids neuropeptides precursors signamre.

CONSENSUS: C-x(3)-C-x(2)-C-x(2)-[KRH]-x(6,7)-[LIF]-[DN]-x(3)-C-x-[LIVM]-[EQ]-C-

CONSENSUS: [EQ]-x(8)-W-x(2)-C

NAME: Pancreatic hormone family signamre

CONSENSUS: [FY]-x(3)-[LIVM]-x(2)-Y-x(3)-[LIVMFY]-x-R-x-R-[YF]

NAME Parathyroid hormone family signamre. CONSENSUS: V-S-E-x-Q-x(2)-H-x(2)-G.

NAME: Pyrokinins signamre. CONSENSUS F-[GSTV]-P-R-L-[G>]

NAME: Somatotropin, prolactin and related hormones signamre 1.

CONSENSUS: C-x-[ST]-x(2)-[LIVMFY]-x-[LIVMSTA]-P-x(5)-[TALIV]-x(7)-[LIVMFY]-x(6)-

CONSENSUS: [LIVMFY]-x(2)-[STA]-W

NAME. Somatotropin, prolactin and related hormones signamre 2

CONSENSUS: C-[LIVMFY]-x(2)-D-[LIVMFYSTA]-x(5)-[LIVMFY]-x(2)-[LrVMFYT]-x(2)-C

NAME: Tachykinin family signamre. CONSENSUS- F-[IVFY]-G-[LM]-M-[G > ]

NAME: Thymosin beta-4 family signamre CONSENSUS K-L-K-K-T-E-T-Q-E-K-N

NAME Urotensin II signamre CONSENSUS C-F- -K-Y-C.

NAME: Cecropin family signamre

CONSENSUS. W-x(0,2)-[KDN]-x(2)-K-[KRE]-[LI]-E-[RKN].

NAME: Mammalian defensins signamre. CONSENSUS : C-x-C-x(3 ,5)-C-x(7)-G-x-C-x(9)-C-C .

NAME: Arthropod defensins signature

CONSENSUS C-x(2,3)-[HN]-C-x(3,4)-[GR]-x(2)-G-G-x-C-x(4,7)-C-x-C

NAME- Cathelicidins signamre 1

CONSENSUS Y-x-[ED]-x-V-x-[RQ]-A-[LIVMA]-[DQG]-x-[LIVMFY]-N-[EQ]

NAME: Cathelicidins signamre 2.

CONSENSUS: F-x-[LIVM]-K-E-T-x-C-x(10)-C-x-F-[KR]-[KE]

NAME: Endothelin family signamre. CONSENSUS: C-x-C-x(4)-D-x(2)-C-x(2)-[FY]-C.

NAME: Plant thionins signamre CONSENSUS: C-C-x(5)-R-x(2)-[FY]-x(2)-C.

NAME: Gamma-thionins family signamre

CONSENSUS- [KR]-x-C-x(3)-[SV]-x(2)-[FYWH]-x-[GF]-x-C-x(5)-C-x(3)-C.

NAME: Snake toxms signamre.

CONSENSUS: G-C-x(l,3)-C-P-x(8,10)-C-C-x(2)-[PDEN].

NAME: Myotoxins signamre.

CONSENSUS K-x-C-H-x-K-x(2)-H-C-x(2)-K-x(3)-C-x(8)-K-x(2)-C-x(2)-[RK]-x-K-C-C-K-K.

NAME: Scorpion short toxins signamre.

CONSENSUS C-x(3)-C-x(6,9)-[GAS]-K-C-[IMQT]-x(3)-C-x-C.

NAME: Heat-stable enterotoxins signamre. CONSENSUS C-C-x(2)-C-C-x-P-A-C-x-G-C. NAME Aerolysin type toxins signamre CONSENSUS [KT]-x(2)-N-W-x(2)-T-[DN]-T

NAME Shiga/πcin ribosomal inactivating toxins active site signature

CONSENSUS [LIVMA]-x-[LIVMSTA](2)-x-E-[SAGV]-[STAL]-R-[FY]-[RKNQS]-x-[LIVM]-[EQS]-

CONSENSUS x(2)-[LIVMF]

NAME Channel forming cohcins signamre CONSENSUS T-x(2)-W-x-P-[LIVMFY](3)-x(2)-E

NAME Hok gef family cell toxic proteins signamre

CONSENSUS [LIVMA](4)-C-[LIVMFA]-T-[LIVMA](2)-x(4)-[LIVM]-x-[RG]-x(2)-L-[CY]

NAME Staphylococcal enterotoxin Streptococcal pyrogemc exotoxin signamre 1 CONSENSUS Y-G-G-[LIV]-T-x(4)-N

NAME Staphyloccocal enterotoxin/Streptococcal pyrogemc exotoxin signamre 2 CONSENSUS K-x(2)-[LrV]-x(4)-[LIV]-D-x(3)-R-x(2)-L-x(5)-[LIV]-Y

NAME Thiol-activated cytolysins signamre CONSENSUS [RK]-E-C-T-G-L-x-W-E-W-W-[RK]

NAME Membrane attack complex components / perform signamre CONSENSUS Y-x(6)-[FY]-G-T-H-[FY]

NAME Pancreatic trypsin inhibitor (Kunitz) family signamre CONSENSUS F-x(3)-G-C-x(6)-[FY]-x(5)-C

NAME Bowman-Birk serine protease inhibitors family signamre

CONSENSUS C-x(5,6)-[DENQKRHSTA]-C-[PASTDH]-[PASTDK]-[ASTDV]-C-[NDKS]-[DEKRHSTA]-C

NAME Kazal serine protease inhibitors family signamre CONSENSUS C-x(7)-C-x(6)-Y-x(3)-C-x(2, 3)-C

NAME Soybean trypsin inhibitor (Kunitz) protease inhibitors family signamre CONSENSUS [LIVM]-x-D-x-[EDNTY]-[DG]-[RKHDENQ]-x-[LIVM]-x(5)-Y-x-[LIVM]

NAME Serprns signamre

CONSENSUS [LIVMFY]-x-[LIVMFYAC]-[DNQ]-[RKHQS]-[PST]-F-[LIVMFY]-[LIVMFYC]-x-

CONSENSUS [LIVMFAH]

NAME Potato inhibitor I family signamre

CONSENSUS [FYW]-P-[EQH]-[LIV](2)-G-x(2)-[STAGV]-x(2)-A

NAME Squash family of senne protease inhibitors signature CONSENSUS C-P-x(5)-C-x(2)-D-x-D-C-x(3)-C-x-C

NAME Streptomyces subtihsin-type inhibitors signamre CONSENSUS C-x-P-x(2,3)-G-x-H-P-x(4)-A-C-[ATD]-x-L

NAME Cysteme proteases inhibitors signature

CONSENSUS [GSTEQKRV]-Q-[LIVT]-[VAF]-[SAGQ]-G-X-[LIVMNK]-X(2)-[LΓVMFY]-X-[LIVMFYA]-

CONSENSUS [DENQKRHSIV]

NAME Tissue inhibitors of metalloproteinases signamre CONSENSUS C-x-C-x-P-x-H-P-Q-x-A-F-C

NAME Cereal trypsin/alpha-amylase inhibitors family signamre

CONSENSUS C-x(4)-[SAGD]-x(4)-[SPAL]-[LF]-x(2)-C-[RH]-x-[LIVMFY](2)-x(3,4)-C

NAME Alpha-2-macroglobulιn family thiolester region signature CONSENSUS [PG]-x-[GS]-C-[GA]-E-[EQ]-x-[LIVM]

NAME Disintegnns signamre

CONSENSUS C-x(2)-G-x-C-C-x-[NQRS]-C-x-[FM]-x(6)-C-[RK]

NAME Lambdoid phages regulatory protein CIII signamre CONSENSUS E-S-x-L-x-R-x(2)-[KR]-x-L-x(4)-[KR](2)-x(2)-[DE]-x-L

NAME Chaperonins cpn60 signamre

CONSENSUS A-[AS]-x-[DEQ]-E-x(4)-G-G-[GA]

NAME Chaperomns cpnlO signamre

CONSENSUS [LIVMFY]-x-P-[ILT]-x-[DEN]-[KR]-[LιVMFA](3)-[KREQ]-x(8,9)-[SG]-x- CONSENSUS [LIVMFY](3)

NAME Chaperonms TCP-1 signamre 1

CONSENSUS [RKEL]-[ST]-x-[LMFY]-G-P-x-[GSA]-x-x-K-[LIVMF](2)

NAME Chaperomns TCP-1 signamre 2

CONSENSUS [LIVM]-[TS]-[NK]-D-[GA]-[AVNHK]-[TAV]-[LIVM](2)-x(2)-[LIVM]-x-[LIVM]-x-

CONSENSUS [SNHHPQH]

NAME Chaperomns TCP-1 signamre 3

CONSENSUS Q-[DEK]-x-x-[LIVMGTA]-[GA]-D-G-T

NAME Heat shock hsp20 proteins family profile

NAME Heat shock hsp70 proteins family signamre 1 CONSENSUS [IV]-D-L-G-T [ST]-x-[SC]

NAME Heat shock hsp70 proteins family signamre 2

CONSENSUS [LIVMF]-[LIVMFY]-[DN]-[LIVMFS]-G-[GSH]-[GS]-[AST]-x(3)-[ST]-[LIVM]-

CONSENSUS [LIVMFC]

NAME Heat shock hsp70 proteins family signamre 3

CONSENSUS [LIVMY]-x-[LIVMF]-x-G-G-x-[ST]-x-[LIVM]-P-x-[LIVM]-x-[DEQKRSTA]

NAME Heat shock hsp90 proteins family signamre CONSENSUS Y-x-[NQH]-K-[DE]-[IVA]-F-L-R-[ED]

NAME Chaperomns clpA/B signamre 1

CONSENSUS D-[AIHSGA]-N-[LIVMF](2)-K-[PT]-x-L-x(2)-G

NAME Chaperomns clpA/B signamre 2

CONSENSUS R-[LIVMFY]-D-x-S-E-[LIVMFY]-x-E-[KRQ]-x-[STA]-x-[STA]-[KR]-[LIVM]-x-G-

CONSENSUS [STA]

NAME Nt-dnaJ domain signamre

CONSENSUS [FY]-x(2)-[LrVMA]-x(3)-[FYWHNT]-[DENQSA]-x-L-x-[DN]-x(3)-[KR]-x(2)-[FYI]

NAME dnaJ domain profile

NAME CXXCXGXG dnaJ domain signamre

CONSENSUS C-[DEGSTHKR]-x-C-x-G-x-[GK]-[AGSDM]-x(2)-[GSNKR]-x(4,6)-C-x(2,3)-C-x-G-x-G

NAME grpE protem signamre

CONSENSUS [FL]-[DN]-[PHEA]-x(2)-[HM]-x-A-[LIVMTN]-x(16,20) G-[FY]-x(3)-[DEG]-x(2)-

CONSENSUS [LIVM]-[RI]-x-[SA]-x-V-x-[IV]

NAME Bacterial type II secretion system protein C signamre

CONSENSUS P-x(6)-F-x(4)-L-x(3)-D-[LIVM]-A-[LIVM]-x-[LIVM]-N-x-[LIVM]-x-L

NAME Bacterial type II secretion system protein D signamre

CONSENSUS [GR]-[DEQKG]-[STVM]-[LIVMA](3)-[GA]-G-[LIVMFY]-x( 11)-[LIVM]-P-

CONSENSUS [LIVMFYWGS]-[LIVMF]-[GSAE]-x-[LIVM]-P-[LIVMFYW](2)-x(2)-[LV]-F

NAME Bacterial type II secretion system protein E signamre CONSENSUS [LIVM]-R-x(2)-P-D-x-[LIVM](3)-G-E-[LIVM]-R-D

NAME Bacterial type II secretion system protein F signamre

CONSENSUS [KRQ]-[LIVMA]-x(2)-[SAIV]-[LIVM]-x-[TY]-P-x(2)-[LrVM]-x(3)-[STAGV]-x(6)-

CONSENSUS [LMY]-x(3)-[LIVMF](2)-P

NAME Bacterial type II secretion system protein N signamre CONSENSUS G-T-L-W-x-G-x( 11 )-L-x(4)-W

NAME Bacterial export FHIPEP family signature

CONSENSUS R-[LΓVM]-[GSA]-E-V-[GSA]-A-R-F-[STV]-L-D-[GSA]-M-P-G-K-Q-M-[GSA]-I-D-

CONSENSUS [GSA]-D

NAME Protein secA signatures

CONSENSUS [rV]-x-[IV]-[SA]-T-[NQ]-M-A-G-R-G-x-D-I-x-L

NAME Protein secY signamre 1

CONSENSUS [GST]-[LIVMF](2)-x-[LIVM]-G-[LIVM]-x-P-[LIVMFY](2)-x-[AS]-[GSTQ]-

CONSENSUS [LIVMFAT](3)-Q-[LrVMFA](2) NAME Protein secY signamre 2

CONSENSUS [LIVMFY ](2)-x-[DE]-x-[LIVMF]-[STN]-x(2)-G-[LIVMF]-[GST]-[NST]-G-x-[GST]-

CONSENSUS [LIVMF](3)

NAME Protein secE/sec61 -gamma signamre

CONSENSUS [LIVMFY]-x(2)-[DENQGA]-x(4)-[LIVMTA]-x-[KRV]-x(2)-[KW]-P-x(3)-[SEQ]-x(7)-

CONSENSUS [LIVTHLIVGAHLIVFGAST]

NAME Gram-negative pill assembly chaperone signamre

CONSENSUS [LIVMFY]-[APN]-x-[DNS]-[KREQ]-E-[STR]-[LIVMAR]-x-[FYWT]-x-[NC]-[LIVM]-

CONSENSUS x(2)-[LIVM]-P-[PAS]

NAME Fimbπal biogenesis outer membrane usher protein signamre

CONSENSUS [VL]-[PASQ]-[PAS]-G-[PAD]-[FY]-x-[LI]-[DNQSTAP]-[DNH]-[LIVMFY]

NAME SRP54-type proteins GTP-bindmg domain signamre

CONSENSUS P-[LrVM]-x-[FYL]-[LIVMAT]-[GS]-x-[GS]-[EQ]-x(4)-[LIVMF]

NAME Cytochrome c oxidase assembly factor COXlO/ctaB/cyoE signamre CONSENSUS [ED]-x-D-x(2)-M-x-R-T-x(2)-R-x(4)-G

NAME Cyclin-dependent kinases regulatory subunits signamre 1

CONSENSUS Y-S-x-[KR]-Y-x-[DE](2)-x-[FY]-E-Y-R-H-V-x-[LV]-[PT]-[KRP]

NAME Cyclin-dependent kinases regulatory subunits signamre 2 CONSENSUS H-x-P-E-x-H-[rV]-L-L-F-[KR]

NAME Pentaxin family signamre CONSENSUS H-x-C-x-[ST]-W-x-[ST]

NAME Immunoglobulins and major histocompatibility complex proteins signamre CONSENSUS [FY]-x-C-x-[VA]-x-H

NAME Pπon protein signamre 1

CONSENSUS A-G-A-A-A-A-G-A-V-V-G-G-L-G-G-Y

NAME Pπon protein signamre 2

CONSENSUS E-x-[ED]-x-K-[LIVM](2)-x-[KR]-[LIVM](2)-x-[QE]-M-C-x(2)-Q-Y

NAME Cyclins signamre

CONSENSUS R-x(2)-[LIVMSA]-x(2)-[FYWS]-[LIVM]-x(8)-[LIVMFC]-x(4)-[LIVMFYA]-x(2)-

CONSENSUS [STAGC]-[LIVMFYQ]-x-[LIVMFYC]-[LIVMFY]-D-[RKH]-[LIVMFYW]

NAME Proliferating cell nuclear antigen signamre 1

CONSENSUS [GA]-[LIVMF]-x-[LrVMA]-x-[SAV]-[LrVM]-D-x-[NSAE]-[HKR]-rVI]-x-[LY]-

CONSENSUS [VGA]-x-[LIVM]-x-[LIVM]-x(4)-F

NAME Proliferating cell nuclear antigen signamre 2

CONSENSUS [RKA]-C-[DE]-[RH]-x(3)-[LIVMF]-x(3)-[LIVM]-x-[SGAN]-[LrVMF]-x-K-

CONSENSUS [LIVMF](2)

NAME Acπn-depolymeπzing proteins signature

CONSENSUS P-[DE]-x-[SA]-x-[LIVMT]-[KR]-x-[KR]-M-[LIVM]-[YA]-[STA](3)-x(3)-[LIVMF]-

CONSENSUS [KR]

NAME BCL2-hke apoptosis inhibitors (spans part of BH3, BH1 and BH2)

NAME Apoptosis regulator, Bcl-2 family BH1 domain signamre

CONSENSUS [LVME]-[FT]-x-[GSD]-[GL]-x( 1 ,2)-[NS]-[YW]-G-R-[LIV]-[LIVC]-[GAT]-

CONSENSUS [LIVMF](2)-x-F-[GSAE]-[GSARY]

NAME Apoptosis regulator, Bcl-2 family BH2 domain signamre

CONSENSUS W-[LIM]-x(3)-[GR]-G-[WQ]-[DENSAV]-x-[FLGA]-[LIVFTC]

NAME Apoptosis regulator, Bcl-2 family BH3 domam signamre

CONSENSUS [LrVAT]-x(3)-L-[KARQ]-x-[IVAL]-G-D-[DESG]-[LIMFV]-[DENSHQ]-[LVSHROJ-

CONSENSUS [NSR]

NAME Apoptosis regulator, Bcl-2 family BH4 domain signamre

CONSENSUS [DS]-[NT]-R-[AE]-[LI]-V-x-[KD]-[FY]-[LIV]-[GHS]-Y-K-L-[SR]-Q-[RK]-G-

CONSENSUS [HY]-x-[CW]

NAME Apoptosis regulator, Bcl-2 family BH4 domain profile NAME Arrestins signamre

CONSENSUS [FY]-R-Y-G-X-[DE](2)-X-[DE]-[LΓVM](2)-G-[LIVM]-X-F-X-[RK]-[DEQ]-[LΓVM]

NAME AAA-protein family signamre

CONSENSUS [LΓVMT]-X-[LΓVMT]-[LIVMF]-X-[GATMC]-[ST]-[NS]-X(4)-[LIVM]-D-X-A-[LIFA]-

CONSENSUS x-R

NAME Ubiquitin domain signamre

CONSENSUS K-X(2)-[LIVM]-X-[DESAK]-X(3)-[LIVM]-[PA]-X(3)-Q-X-[LIVM]-[LIVMC]-

CONSENSUS [LIVMFY]-x-G-x(4)-[DE]

NAME Ubiquitin domain profile

NAME ADP-ribosylation factors family signamre

CONSENSUS [HRQT]-x-[FYWI]-x-[LIVM]-x(4)-A-x(2)-G-x(2)-[LIVM]-x(2)-[GSA]-[LIVMF]-x-

CONSENSUS [WKHLIVM]

NAME GTP-binding nuclear protein ran signamre CONSENSUS D-T-A-G-Q-E-K-[LF]-G-G-L-R-[DE]-G-Y-Y

NAME SARI family signamre

CONSENSUS R-x-[LIVM]-E-V-F-M-C-S-[LIVM](2)-x-[KRQ]-x-G-Y-x-E-[AG]-[FI]-x-W-[LIVM]-

CONSENSUS x-Q-Y

NAME Band 7 protein family signamre

CONSENSUS R-x(2)-[LIV]-[SAN]-x(6)-[LIV]-D-x(2)-T-x(2)-W-G-[LIV]-[KRH]-[LIV]-x-

CONSENSUS [KR]-[LIV]-E-[LIV]-[KR]

NAME Trp-Asp (WD) repeats signamre

CONSENSUS [LIVMSTAC]-[LIVMFYWSTAGC]-[LIMSTAG]-[LIVMSTAGC]-x(2)-[DN]-x(2)-

CONSENSUS [LIVMWSTAC]-x-[LIVMFSTAG]-W-[DEN]-[LIVMFSTAGCN]

NAME G-protein gamma subunit profile

NAME Ras GTPase-activating proteins signamre

CONSENSUS [GSN]-X-[LΓVMF]-[FY]-[LIVMFY]-R-[LIVMFY](2)-[GACN]-P-[AV]-[LΓV](2)-

CONSENSUS [SGAN]-P

NAME Ras GTPase-activating proteins profile

NAME Guanine-nucleotide dissociation stimulators CDC24 family signamre

CONSENSUS L-x(2)-[LIVMFYW]-L-x(2)-P-[LIVM]-x(2)-[LIVM]-x-[KRS]-x(2)-L-x-[LIVM]-x-

CONSENSUS [DEQ]-[LIVM]-x(3)-[ST]

NAME Guamne-nucleotide dissociation stimulators CDC25 family signamre CONSENSUS [GAP]-[CT]-V-P-[FY]-x(4)-[LIVMFY]-x-[DN]-[LIVM]

NAME MARCKS family signamre 1 CONSENSUS G-Q-E-N-G-H-V-[KR]

NAME MARCKS family phosphorylation site domain

CONSENSUS E-T-P-K(5)-x(0, 1 )-F-S-F-K-K-x-F-K-L-S-G-x-S-F-K-[KR]-[NS]-[KR]-K-E

NAME Stathmin family signamre 1

CONSENSUS P-[KQ]-[KR](2)-[DE]-x-S-L-[EG]-E

NAME Stathmin family signamre 2 CONSENSUS A-E-K-R-E-H-E-[KR]-E-V

NAME GTP-bmding elongation factors signamre

CONSENSUS D-[KRSTGANQFYW]-x(3)-E-[KRAQ]-x-[RKQD]-[GC]-[IVMK]-[ST]-[IV]-x(2)-

CONSENSUS [GSTACKRNQ]

NAME Elongation factor 1 beta/beta '/delta chain signamre 1 CONSENSUS [DE]-[DEG]-[DE](2)-[LIVMF]-D-L-F-G

NAME Elongation factor 1 beta/beta '/delta chain signamre 2 CONSENSUS V-Q-S-x-D-[LIVM]-x-A-[FWM]-[NQ]-K-[LIVM]

NAME Elongation factor 1 gamma chain profile

NAME Elongation factor Ts signamre 1

CONSENSUS L-R-x(2)-T-[GDQ]-x-[GS]-[LIVMF]-x(0,l)-[DENKAC]-x-K-[KRNEQS]-[AV]-L NAME Elongation factor Ts signamre 2

CONSENSUS E-[LIVM]-N-[SCV]-[QE]-T-D-F-V-[SA]-[KRN]

NAME Elongation factor P signamre

CONSENSUS K-x-A-x(4)-G-x(2)-[LIV]-x-V-P-x(2)-[LIV]-x(2)-G

NAME Eukaryotic initiation factor IA signamre

CONSENSUS [IM]-x-G-x-[GS]-[KRH]-x(4)-[CL]-x-D-G-x(2)-R-x(2)-[RH]-I-x-G

NAME Eukaryotic initiation factor 4E signamre

CONSENSUS [DE]-[IFY]-x(2)-F-[KR]-x(2)-[LIVM]-x-P-x-W-E-[DV]-x(5)-G-G-[KR]-W

NAME Eukaryotic initiation factor 5A hypusine signamre CONSENSUS [PTJ-G-K-H-G-x-A-K

NAME Initiation factor 2 signamre

CONSENSUS G-x-[LIVM]-x(2)-L-[KR]-[KRHNS]-x-K-x(5)-[LIVM]-x(2)-G-x-[DEN]-C-G

NAME Initiation factor 3 signamre

CONSENSUS [KR]-[LIVM](2)-[DN]-[FY]-[GSN]-[KR]-[LrVMFYS]-x-[FY]-[DEQT]-x(2)-[KR]

NAME Translation initiation factor SUI1 signamre

CONSENSUS [LIVM]-[EQ]-[LIVM]-Q-G-[DEN]-[KHQ]-[KRV]

NAME Prokaryotic-type class I peptide chain release factors signamre CONSENSUS [AR]-[STA]-x-G-x-G-G-Q-[HNGCS]-V-N-x(3)-[ST]-A-[IV]

NAME Transcription termination factor nusG signamre CONSENSUS [LIVM}-F-G-[KRW]-x-T-P-[IV]-x-[LIVM]

NAME Calponin family repeat

CONSENSUS [LIVM]-x-[LS]-Q-[MAS]-G-[STY]-[NT]-[KRQ]-x(2)-[STN]-Q-x-G-x(3,4)-G

NAME CAP protein signamre 1

CONSENSUS [LIVM](2)-x-R-L-[DE]-x(4)-R-L-E

NAME CAP protein signamre 2

CONSENSUS D-[LrVMFY]-x-E-x-[PA]-x-P-E-Q-[LIVMFY]-K

NAME Calreticuhn family signamre 1

CONSENSUS [KRHN]-x-[DEQN]-[DEQNK]-x(3)-C-G-G-[AG]-[FY]-[LIVM]-[KN]-[LrVMFY](2)

NAME Calreticuhn family signamre 2 CONSENSUS [LIVM](2)-F-G-P-D-x-C-[AG]

NAME Calreticuhn family repeated motif signamre

CONSENSUS [IV]-x-D-x-[DENST]-x(2)-K-P-[DEH]-D-W-[DEN]

NAME Calsequestπn signamre 1

CONSENSUS [EQ]-[DE]-G-L-[DN]-F-P-x-Y-D-G-x-D-R-V

NAME Calsequestπn signamre 2

CONSENSUS [DE]-L-E-D-W-[LIVM]-E-D-V-L-x-G-x-[LIVM]-N-T-E-D-D-D

NAME S-100/ICaBP type calcium binding protein signamre

CONSENSUS [LIVMFYW](2)-x(2)-[LK]-D-x(3)-[DN]-x(3)-[DNSG]-[FY]-x-[ES]-[FYVC]-x(2)-

CONSENSUS [LIVMFSHLIVMF]

NAME Hemolysin-type calcium-binding region signamre CONSENSUS D-x-[LI]-x(4)-G-x-D-x-[LI]-x-G-G-x(3)-D

NAME HlyD family secretion proteins signamre

CONSENSUS [LIVM]-x(2)-G-[LM]-x(3)-[STGAV]-x-[LIVMT]-x-[LIVMT]-[GE]-x-[KR]-x-

CONSENSUS [LIVMFYW](2)-x-[LIVMFYW](3)

NAME P-π protein urydylation site CONSENSUS Y-[KR]-G-[AS]-[AE]-Y

NAME P-II protein C-terminal region signamre

CONSENSUS [ST]-X(3)-G-[DY]-G-[KR]-[IV]-[FW]-[LΓVM]-X(2)-[LIVM]

NAME 14-3-3 proteins signamre 1

CONSENSUS R-N-L-[LIV]-S-[VG]-[GA]-Y-[KN]-N-[IVA] NAME 14-3-3 proteins signamre 2

CONSENSUS Y-K-[DE]-S-T-L-I-[IM]-Q-L-[LF]-[RHC]-D-N-[LF]-T-[LS]-W-[TAN]-[SAD]

NAME ATP1G1 / PLM / MAT8 family signamre

CONSENSUS [DNS]-x-F-x-Y-D-x(2)-[ST]-[LIVM]-[RQ -x(2)-G

NAME BTG1 family signature 1

CONSENSUS Y-x(2)-[HP]-W-[FY]-[AP]-E-x-P-x-K-G-x-[GA]-[FY]-R-C-[IV]-[RH]-[IV]

NAME BTG1 family signamre 2

CONSENSUS [LV]-P-x-[DE]-[LM]-[ST]-[LIVM]-W-[IV]-D-P-x-E-V-[SC]-x-[RQ]-x-G-E

NAME Cullin family signature

CONSENSUS [LIV]-K-x(2)-[LIV]-x(2)-L-I-[DEQ]-[KRHNQ]-x-Y-[LIVM]-x-R-x(6,7)-[FY]-x-

CONSENSUS Y-x-[SA] >

NAME Cullin family profile

NAME Enhancer of rudimentary signamre

CONSENSUS Y-D-I-[SA]-x-L-[FY]-x-F-[IV]-D-x(3)-D-[LIV]-S

NAME G10 protein signamre 1

CONSENSUS L-C-C-x-[KR]-C-x(4)-[DE]-x-N-x(4)-C-x-C-R-V-P

NAME G10 protein signamre 2

CONSENSUS C-x-H-C-G-C-[KRH]-G-C-[SA]

NAME Glucokinase regulatory protein family signamre

CONSENSUS G-[PA]-E-x-[LIV]-[STA]-G-S-[ST]-R-[LIVM]-K-[STGA](3)-x(2)-K

NAME GTP1/OBG family signamre

CONSENSUS D-[LIVM]-P-G-[LIVM](2)-[DEY]-[GN]-A-x(2)-G-x-G

NAME HIT family signature

CONSENSUS [NQA]-x(4)-[GAV]-x-[QF]-x-[LIVM]-x-H-[LIVMFYT]-H-[LIVMFT]-H-[LIVMF](2)-

CONSENSUS [PSGA]

NAME Caserns alpha/beta signamre CONSENSUS C-L-[LV]-A-x-A-[LVF]-A

NAME Clathπn adaptor complexes medium cham signamre 1

CONSENSUS [IVT]-[GSP]-W-R-x(2,3)-[GAD]-x(2)-[HY]-x(2)-N-x-[LIVMAFY](3)-D-[LrVM]-

CONSENSUS [LIVMT]-E

NAME Clathrm adaptor complexes medium cham signamre 2 CONSENSUS [LIV]-x-F-I-P-P-x-G-x-[LIVMFY]-x-L-x(2)-Y

NAME Clathrm adaptor complexes small chain signamre CONSENSUS [LIVM](2)-Y-[KR]-x(4)-L-Y-F

NAME Ependynuns signamre 1

CONSENSUS F-E-E-G-x-[LIVMF]-Y-[ED]-I-D-x(2)-N-[QE]-S-C-[RKH](2)

NAME Ependynuns signamre 2

CONSENSUS [QE]-[LIVMA]-F-X(2)-P-[STA}-[FY]-C-[DE]-[GA]-[LΓVM]-X(2)-[DE](2)

NAME Syntaxin / epimorphin family signamre

CONSENSUS [RQ]-x(3)-[LIVMA]-x(2)-[LIVM]-[ESH]-x(2)-[LIVMT]-x-[DEVM]-[LIVM]-x(2)-

CONSENSUS [LIVM]-[FS]-x(2)-[LIVM]-x(3)-[LIVT]-x(2)-Q-[GADEQ]-x(2)-[LIVM]-[DNQT]-x-

CONSENSUS [LIVMF]-[DESV]-x(2)-[LIVM]

NAME Extracellular proteins SCP/Tpx-l/Ag5/PR-l/Sc7 signamre 1 CONSENSUS [GDER]-H-[FYWH]-T-Q-[LrVM](2)-W-x(2)-[STN]

NAME Extracellular proterns SCP/Tpx-l/Ag5/PR-l/Sc7 signamre 2

CONSENSUS [LIVMFYH]-[LIVMFY]-x-C-[NQRHS]-Y-x-[PARH]-x-[GL]-N-[LIVMFYWDN]

NAME Fetuin family signamre 1

CONSENSUS C-x(56)-C-x(10)-C-x(13)-C-x( 17, 18)-C-x(13)-C-x(2)-C-x(58)-C-x(10, 11)-

CONSENSUS C-x(10,12)-C-x(16,22)-C

NAME Fetuin family signamre 2 CONSENSUS L-E-T-x-C-H-x-L-D-P-T-P NAME Legume lectins beta-chain signamre CONSENSUS [LIV]-[STAG]-V-[DEQV]-[FLI]-D-[ST]

NAME Legume lectins alpha-chain signamre

CONSENSUS [LIV]-x-[EDQ]-[FYWKR]-V-x-[LIV]-G-[LF]-[ST]

NAME Vertebrate galactoside-bmding lectin signamre

CONSENSUS W-[GEK]-x-[EQ]-x-[KRE]-x(3,6)-[PCTF]-[LIVMF]-[NQEGSKV]-x-[GH]-x(3)-

CONSENSUS [DENKHS]-[LIVMFC]

NAME Lysosome-associated membrane glycoproteins duplicated domain signamre CONSENSUS [STA]-C-[LIVM]-[LIVMFYW]-A-x-[LIVMFYW]-x(3)-[LIVMFYW]-x(3)-Y

NAME LAMP glycoproteins transmembrane and cytoplasmic domain signamre

CONSENSUS C-x(2)-D-x(3,4)-[LIVM](2)-P-[LrVM]-x-[LIVM]-G-x(2)-[LIVM] x-G-[LIVM](2)-

CONSENSUS x-[LIVM](4)-A-[FY]-x-[LIVM]-x(2)-[KR]-[RH]-x( 1 ,2)-[STAG](2)-Y-[EQ]

NAME Glycophoπn A signamre

CONSENSUS I-I-x-[GAC]-V-M-A-G-[LIVM](2)

NAME PMP-22 / EMP / MP20 family signamre 1

CONSENSUS [LrVMF](4)-[SA]-T-x(2)-[DNKS]-x-W-x(9, 13)-[LIV]-W-x(2)-C

NAME PMP-22 / EMP / MP20 family signamre 2

CONSENSUS [RQ]-[AV]-x-M-[rV]-L-S-x-[Lη-x(4)-[GSA]-[LIVMF](3)

NAME Oxysterol-binding protein family signamre CONSENSUS E [KQ]-x-S-H-[HR]-P-P-x-[STACF]-A

NAME Yeast PIR proteins repeats signamre

CONSENSUS S-Q-[IV]-[STGNH]-D-G-Q-[LIV]-Q-[AIV]-[STA]

NAME Seminal vesicle protein I repeats signamre

CONSENSUS [IVM]-x-G Q D-x-V K-x(5)-[KN]-G-x(3)-[STLV]

NAME Seminal vesicle protein II repeats signamre CONSENSUS [GSA]-Q-x-K-S-[FY] x Q-x-K [SA]

NAME Serum amyloid A proteins signamre

CONSENSUS A-R-G-N-Y-[ED]-A-x-[QKR]-R-G-x-G-G-x-W-A

NAME Spermadhesins family signamre 1 CONSENSUS C-G-x(2)-[LI]-x(4)-G-x-I-x(9) C-x-W-T

NAME Spermadhesins family signamre 2

CONSENSUS C-x-K-E-x-[LIVM]-E-[LIVM]-x-[DE]-x(3)-[GS]-x(5)-K-x-C

NAME Stress-induced proteins SRP1/TIP1 family signamre CONSENSUS P-W-Y-[ST](2)-R-L

NAME Glypicans signamre

CONSENSUS C-x(2)-C-x-G-[LIVM]-x(4)-P-C-x(2)-[FY]-C-x(2)-[LIVM]-x(2)-G-C

NAME Syndecans signature

CONSENSUS [FY]-R-[IM]-[KR]-K(2)-D-E-G-S-Y

NAME Tissue factor signamre

CONSENSUS W-K-x-K-C-x(2)-T-x-[DEN]-T-E-C-D-[LIVM]-T-D-E

NAME Translaπonally controlled tumor protein signamre 1

CONSENSUS [IA]-G-[GAS]-N-[PA]-S-A-E-[GDE]-[PAGE]-x(0,l)-[DEG]-x-[DEN]-x(2)-[DE]

NAME Translaπonally controlled tumor protein signamre 2

CONSENSUS [FL]-[FY]-[rVT]-G-E-x-[MA]-x(2,5)-[DEN]-[GAS]-x-[LV]-[AV]-x(3)-[FY]-[KR]-

CONSENSUS [DE]

NAME Tub family signamre 1

CONSENSUS F-[KHQ]-G-R-V-[ST]-x-A-S-V-K-N-F-Q

NAME Tub family signamre 2

CONSENSUS A-F-[AG]-I-[SAC]-[LIVM]-[ST]-S-F-x-[GST]-K-x-A-C-E

NAME HCP repeats signamre CONSENSUS H-R-H-R-G-H-x(2)-[DE](7) NAME: Bactenal ice-nucleation proteins octamer repeat CONSENSUS. A-G-Y-G-S-T-x-T.

NAME- Cell cycle proteins ftsW / rodA / spoVE signamre.

CONSENSUS [NV]-x(5)-[GTR]-[LIVMA]-x-P-[PTLIVM]-x-G-[LIVM]-x(3)-[LIVMFW](2)-S-[YSA]-

CONSENSUS G-G-[STN]-[SA].

NAME: Enterobacteπal virulence outer membrane protein signamre 1 CONSENSUS G-[LIVMFY]-N-[LIVM]-K-Y-R-Y-E.

NAME: Enterobacteπal virulence outer membrane protein signamre 2. CONSENSUS. [FYW]-x(2)-G-x-G-Y-[KR]-F >

NAME: Hydrogenases expression/synthesis hypA family signamre

CONSENSUS: F-[CSA]-[FY]-[DE]-[LIVA](2)-x(3)-[ST]-[LIVM]-x(16)-C-x(2)-C-x(12,15)-

CONSENSUS. C-P-x-C.

NAME: Hydrogenases expression synthesis hupF/hypC family signature. CONSENSUS < M-C-[LIV]-[GA]-[LIV]-P-x-[QKR]-[LIV].

NAME: Staphylocoagulase repeat signamre.

CONSENSUS- A-R-P-x(3)-K-x-S-x-T-N-A-Y-N-V-T-T-x(2)-[DN]-G-x(3)-Y-G

NAME: 11-S plant seed storage proteins signamre

CONSENSUS: N-G-x-[DE](2)-x-[LIVMF]-C-[ST]-x(l 1,12)-[PAG]-D.

NAME: Dehydπns signamre 1

CONSENSUS S(5)-[DE]-x-[DE]-G-x(l ,2)-G-x(0, 1)-[KR](4).

NAME: Dehydπns signamre 2

CONSENSUS: [KR]-[LIM]-K-[DE]-K-[LIM]-P-G.

NAME: Germin family signature.

CONSENSUS. G-x(4)-H-x-H-P-x-A-x-E-[LIVM]

NAME: Oleosins signamre

CONSENSUS- [AG]-[ST]-x(2)-[AG]-x(2)-[LrVM]-[SAD]-T-P-[LIVMF](4)-F-S-P-[LIVM](3)-

CONSENSUS. P-A

NAME Small hydrophilic plant seed proteins signamre. CONSENSUS: G-IEQJ-T-V-V-P-G-G-T.

NAME- Pathogenesis-related proteins Betvl family signamre

CONSENSUS G-x(2)-[LrVMF]-x(4)-E-x(2)-[CSTAEN]-x(8,9)-[GND]-G-[GS]-[CS]-x(2)-K-x(4)-

CONSENSUS [FY].

NAME: Pollen proteins Ole e I family signature. CONSENSUS [EQ -G-x-V-Y-C-D-T-C-R.

NAME: Thaumaπn family signature

CONSENSUS G-x-[GF]-x-C-x-T-[GA]-D-C-x(l ,2)-G-x(2,3)-C.

NAME. Mrp family signature

CONSENSUS W-x(2)-[LIVM]-D-[LrVMY](4)-D-x-P-P-G-T-[GS]-D.

NAME: Glucose inhibited division protein A family signamre 1 CONSENSUS [GS]-P-x-Y-C-P-S-[LIVM]-E-x-K-[LIVM]-x-[KR]-F.

NAME- Glucose inhibited division protein A family signamre 2.

CONSENSUS A-G-Q-X-[NT]-G-X(2)-G-Y-X-E-[SAG](3)-[QS]-G-[LIVM](2)-A-G-[LΓVMT]-N-A.

NAME NOLl/NOP2/sun family signamre.

CONSENSUS- [FV]-D-[KRA]-[LIVMA]-L-x-D-[AV]-P-C-[ST]-[GA].

NAME- PET112 family signamre.

CONSENSUS- [DN]-x-[DN]-R-x(3)-P-L-[LrV]-E-[LIV]-x-[ST]-x-P.

NAME: Protein smpB signamre

CONSENSUS: [TA]-G-[LIVM]-x-L-x-G-x-E-[LIVM]-rKQ]-[SA]-[LrVM].

NAME: Hypothetical cof family signamre 1

CONSENSUS [LIVFYAN]-[LΓVMFA]-X(2)-D-[LΓVMF]-[ND]-G-T-[LIV]-[LVY]-[STANLM]. NAME Hypothetical cof family signamre 2

CONSENSUS: [LIVMFC]-G-D-[GSANQ]-x-N-D-x(3)-[LIMFY]-x(2)-[AV]-x(2)-[GSCP]-x(2)-

CONSENSUS [LMP]-x(2)-[GAS]

NAME- RIO1/ZK632.3/MJ0444 family signamre. CONSENSUS- [LΓVM]-V-H-[GA]-D-L-S-E-[FY]-N-X-[LΓVM]

NAME: SUA5/ycιθ/yrdC family signamre

CONSENSUS- [LIVMTA](3)-[LIVMFYC]-[PG]-T-[DE]-[STA]-x-[FY]-[GA]-[LIVM]-[GS].

NAME. Uncharacteπzed protein family UPFOOOl signamre. CONSENSUS: [FW]-H-[FM]-[IV]-G-x-[LIV]-Q-x-[NKR]-K-x(3)-[LIV]

NAME: Uncharacteπzed protein family UPF0003 signamre

CONSENSUS: G-x-V-x(2)-[LIV]-x(3)-[SA]-x(6)-D-x(3)-[LIVT](3)-P-N-x(2)-[LIVMF](2)-

CONSENSUS: x(5)-N

NAME- Uncharacteπzed protein family UPF0004 signamre.

CONSENSUS: [LIVM]-x-[LIVMT]-x(2)-G-C-x(3)-C-[STAN]-[FY]-C-x-[LIVM]-x(4)-G

NAME- Uncharacteπzed protein family UPF0005 signamre.

CONSENSUS: G-[LIVM](2)-[SA]-x(5,8)-G-x(2)-[LIVM]-G-P-x-L-x(4)-[SAG]-x(4,6)-

CONSENSUS: [LIVM](2)-x(2)-A-x(3)-T-A-[LIVM](2)-F.

NAME- Uncharacteπzed protein family UPF0006 signamre 1. CONSENSUS: [LIVMFY](2)-D-[STA]-H-x-H-[LIVMF]-[DN].

NAME- Uncharacteπzed protein family UPF0006 signamre 2 CONSENSUS P-[LIVM]-x-[LIVM]-H-x-R-x-[TA]-x-[DE] .

NAME Uncharactenzed protein family UPF0006 signamre 3.

CONSENSUS: [LVSA]-[LIVA]-x(2)-[LIVM]-[PS]-x(3)-L-[LIVM]-[LIVMS]-E-T-D-x-P

NAME- Uncharactenzed protein family UPF0007 signamre. CONSENSUS. V-L-[IV]-H-D-[GA]-A-R

NAME: Uncharacteπzed protein family UPF0011 signature CONSENSUS S-D-A-G-x-P-x-[LIV]-[SN]-D-P-G.

NAME: Uncharactenzed protem family UPF0012 signamre. CONSENSUS [GTA]-x(2)-[IVT]-C-Y-D-[LIVM]-x-F-P-x(9)-G

NAME. Uncharacteπzed protein family UPF0015 signamre.

CONSENSUS: [DE]-[LIVMF](3)-R-T-[SG]-G-x(2)-R-x-S-x-[FY]-[LIVM](2)-W-Q

NAME: Uncharactenzed protein family UPF0016 signamre. CONSENSUS- E-[LIVM]-G-D-K-T-F-[LIVMF](2)-A

NAME- Uncharactenzed protem family UPF0017 signamre.

CONSENSUS D-x(8)-[GN]-[LFY]-x(4)-[DET]-[LY]-Y-x(3)-[ST]-x(7)-[IV]-x(2)-[PS]-x-

CONSENSUS: [LIVM]-x-[LIVM]-x(3)-[DN]-D.

NAME: Uncharactenzed protein family UPF0019 signamre.

CONSENSUS- L-P-V-[VT]-[NQL]-F-[AT]-A-G-G-[LIV]-A-T-P-A-D-A-A-[LM].

NAME: Uncharactenzed protein family UPF0020 signamre. CONSENSUS: D-P-[LIVMF]-C-G-[ST]-G-x(3)-[LI]-E.

NAME: Uncharactenzed protein family UPF0021 signamre. CONSENSUS. C-K-x(2)-F-x(4)-E-x(22,23)-S-G-G-K-D.

NAME: Uncharacteπzed protem family UPF0023 signamre CONSENSUS: D-x-D-E-[LIV]-L-x(4)-V-F-x(3)-S-K-G.

NAME: Uncharacteπzed protem family UPF0024 signamre. CONSENSUS G-x-K-D-[KR]-x-A-[LV]-T-x-Q-x-[LIVF]-[SGC].

NAME: Uncharactenzed protem family UPF0025 signamre. CONSENSUS: D-V-[LIV]-x(2)-G-H-[ST]-H-x(12)-[LIVMF]-N-P-G.

NAME Uncharactenzed protem family UPF0027 signamre.

CONSENSUS: Q-[LiVM]-x-N-x-A-x-[LrVM]-P-x-I-x(6)-[LIVM]-P-D-x-H-x-G-x-G-x(2)-[IV]-G.

NAME- Uncharacteπzed protem family UPF0028 signamre. CONSENSUS [GA]-[GS]-G-[GA]-A-R-G-x-[SA]-H-x-G-x(9)-[IV]-x-[IV]-D-x(2)-[GA]-G-x-S-

CONSENSUS x-G

NAME Uncharactenzed protein family UPF0029 signamre

CONSENSUS G-x(2)-[LIVM](2)-x(2)-[LIVM]-x(4)-[LIVM]-x(5)-[LIVM](2)-x-R-[FYW](2)-G-

CONSENSUS G-x(2)-[LIVM]-G Uncharactenzed protein family UPF0030 signamre CONSENSUS [GA]-L-I-[LIV]-P-G-G-E-S-T-[STA]

NAME Uncharacteπzed protein family UPF0031 signature 1

CONSENSUS [SAV]-[IVW]-[LVA]-[LIV]-G-[PNS]-G-L-[GP]-x-[DENQT]

NAME Uncharactenzed protein family UPF0031 signature 2 CONSENSUS [GA]-G-x-G-D-[TV]-[LT]-[STA]-G-x-[LIVM]

NAME Uncharactenzed protein family UPF0032 signamre

CONSENSUS Y-x(2)-F-[LIVMA](2)-x-L-x(4)-G-x(2) F-[EQ]-[LIVMF]-P-[LIVM]

NAME Uncharactenzed protein family UPF0033 signamre CONSENSUS L-[DN]-x(2)-[TAG]-x(2)-C-P-x-P-x-[LIVM]

NAME Uncharactenzed protein family UPF0034 signamre

CONSENSUS [LIVM]-[DNG]-[LIVM]-N-x-G-C-P-x(3)-[LIVMASQ]-x(5)-G-[SAC]

NAME Uncharactenzed protein family UPF0035 signamre CONSENSUS L-L-T-x-R-[SA]-x(3)-R-x(3)-G-x(3)-F-P-G-G

NAME Uncharactenzed protein family UPF0O36 signamre

CONSENSUS H-x-S-G-H-[GA]-x(3)-[DE]-x(3)-[LM]-x(5)-P-x(3)-[LIVM]-P-x-H-G-[DE]

NAME Uncharactenzed protein family UPF0038 signamre CONSENSUS G-x-[LI]-x-R-x(2)-L-x(4)-F-x(8HLrvj-x(5)-P-x-[LIV]

NAME Uncharactenzed protein family UPF0044 signamre

CONSENSUS L-[ST]-x(3)-K-x(3)-[KR] [SGA]-x-[GA] H-x-L-x-P-[LrV]-x(2)-[LrV]-[GA]-

CONSENSUS x(2)-G

NAME Uncharactenzed protein family UPF0047 signamre CONSENSUS S-X(2)-[LIV]-X-[LΓV]-X(2)-G-X(4)-G-T-W-Q-X-[LIV]

NAME Uncharacteπzed protein family UPF0054 signamre CONSENSUS H-[GS]-x-L-H-L-[LI]-G-[FYW]-D-H

NAME Uncharactenzed protein family UPF0057 signamre

CONSENSUS [LΓV]-X-[STA]-[LIVF](3)-P-P-[LIVA]-[GA]-[IV]-X(4)-[GKN]

NAME Hypothetical YER057c/yjjV family signamre

CONSENSUS P-[AT]-R-[SA]-X-[LΓVMY]-X(2)-[AK]-X-L-P-X(4)-[LIVM]-E

NAME Hypothetical hesB/yadR/yfhF family signamre

CONSENSUS F-x-[LIVMFY]-x-N-[PG]-[NSK]-x(4)-C-x-C-[GS]-x-S-F

NAME Hypothetical yabO/yceC/sffiB family signamre

CONSENSUS [NHY]-R-[LI]-D-x(2)-T-[ST]-G-[LIVMA]-[LIVMF](2)-[LIVMFG]-[SGAC]

Claims

We claim:

1. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_16cl6; hfbr2_16f21; hfbr2_16gl8; hfbr2_16il2; hfbr2_16k22; hfbr2_16112; hfbr2_22f21; hfbr2_22hl3; hfbr2_22hl3; hfbr2_22i4; hfbr2_22k3; hfbr2_22k8; hfbr2_23bl0; hfbr2_23b21; hfbr2_23f2; hfbr2_23124; hfbr2_23nl6; hfbr2_23o24; hfbr2_23o5; hfbr2_2a2; hfbr2_2bl7; hfbr2_2b5; hfbr2_2cl; hfbr2_2cl7; hfbr2_2cl8; hfbr2_2dl5; hfbr2_2dl7; hfbr2_2d20; hfbr2_2gl8; hfbr2_2hl; hfbr2_2hl0; hfbr2_2il7; hfbr2_2kl4; hfbr2_2kl9; hfbr2_3bl6; hfbr2_3cl8; hfbr2_3fl6; hfbr2_3g8; hfbr2_312; hfbr2_41ml5; hfbr2_62bll; hfbr2_62fl0; hfbr2_62119; hfbr2_62nl0; hfbr2_62ol7; hfbr2_64al l; hfbr2_64al5; hfbr2_64cl6; hfbr2_64c4; hfbr2_64h6; hfbr2_64i20; hfbr2_64jl8; hfbr2_64k24; hfbr2_64ol6; hfbr2_6al7; hfbr2_6b24; hfbr2_6i20; hfbr2_6ol7; hfbr2_71o20; hfbr2_72bl8; hfbr2_72dl3; hfbr2_72112; hfbr2_72ml6; hfbr2_72nl2; hfbr2_78c24; hfbr2_78dl3; hfbr2_78k24; hfbr2_78n23; hfbr2_7a24; hfbr2_7e22; hfbr2_7j4; hfbr2_82c20; hfbrl_10c20; hfbr2_82el7; hfbrl_10el7; hfbr2_82e4;; hfbrl_10e4; hfbr2_82gl4;; hfbrl_10gl4; hfbr2_82il7;; hfbrl_10; hfbr2_82i24;; hfbrl lO; hfbr2_82ml6;; hfbrl_10; hfbr2_82m6;; hfbrl_10; hfkd2_lj9; hfkd2_24al5; hfkd2_24bl5; hfkd2_24e23; hfkd2_24n20; hfkd2_24p5; hfkd2_3il3; hfkd2_3ol7; hfkd2_46a6; hfkd2_46bl0; hfkd2_46dl3; hfkd2_46j20; hfkd2_46kl9; hfkd2_46m4; hfkd2_47a4; hfkd2_4b6; hfkd2_4c8; hfkd2_4kl4; hfkd2_4ml l; hmcfl lall; hmcfl_lc23; hmcfl_lel5; hmcfl_lgl3; hhtes3_ln3; htes3_14g5; htes3_14h21; htes3_14pl4; htes3_14p7; htes3_15al3; Htes3_15c24; htes3_15c6; htes3_15gl4; htes3_15hl; htes3_15i5; htes3_15jl8; Htes3_15j3; htes3_15kll; htes3_17fl0; htes3_17U7; htes3_17nl2; htes3_17nl8; Htes3_18f3; htes3_1817; htes3_19fl9; htes3_19jl7; htes3_lcl; htes3_lgl3; htes3_lkll; htes3_20c21; htes3_20k2; htes3_20ml8; htes3_21d4; htes3_21jl5; htes3_21116; htes3_21n23; htes3_22c23; htes3_22g2; htes3_22nl3; htes3_23111; htes3_23nl9; Htes3_23nl9; htes3_26g22; htes3_27dl; htes3_27k4; htes3_27ol4; htes3_28dl4; htes3_2al l; htes3_2al7; htes3_2dl5; htes3_2el2; htes3_2fl4; htes3_2g7; htes3_2hl; htes3_2hl5; htes3_2119; htes3_2ml8; htes3_2m20; htes3_2n9; htes3_2ol3; htes3_30f4; Htes3_35b4; htes3_35b5; htes3_35e21; htes3_35g6; htes3_35kl6; htes3_35k24; htes3_35nl2; htes3_35n24; htes3_35n9; htes3_35pl7; htes3_35p22; htes3_4b4; htes3_4fl7; htes3_4f5; htes3_4h6; htes3_4ol9; htes3_50j4; htes3_50n06; htes3_50n23; htes3_6b21; htes3_6cll; htes3_6dl6; htes3_72kl l; Htes3_72kl5; htes3_72pl6; htes3_7b22; htes3_7dl7; htes3_7j3; htes3_7j8; htes3_7pl0; htes3_7p9; htes3_8e24; Htes3_8gl l; Htes3_8g5; htes3_8ml0; Htes3_8p7; Htes3_9e22; Htes3_9i20; Htes3_9k22; hutel_17k7; hutel_18cl2; hutel_18il9; hutel_18i4; hutel lδll; hutel_19fl9; hutel_19gl9; hutel_19g22; hutel_19hl7; hutel_19jll; hutel_li2; hutel_20bl9; hutel_20g21; hutel_20hl3; hutel_20mll; hutel_20m24; hutel_21dl5; hutel_22d2; hutel_22el2; hutel_22n2; hutel_22o2; hutel_23el3; hutel_23gll; hutel_24cl9; hutel_24ell; hutel_24j6; hutel_2h3; their complements; and variants thereof.

2. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_16cl6; hfbr2_16f21; hfbr2_16gl8; hfbr2_16il2; hfbr2_16k22; hfbr2_16112; hfbr2_22f21; hfbr2_22hl3; hfbr2_22hl3; hfbr2_22i4; hfbr2_22k3; hfbr2_22k8; hfbr2_23bl0; hfbr2_23b21; hfbr2_23f2; hfbr2_23124; ; hfbr2_23nl6; hfbr2_23o24; hfbr2_23o5; hfbr2_2a2; hfbr2_2bl7; hfbr2_2b5; hfbr2_2cl; hfbr2_2cl7; hfbr2_2cl8; hfbr2_2dl5; hfbr2_2dl7; hfbr2_2d20; hfbr2_2gl8; hfbr2_2hl; hfbr2_2hlO; hfbr2_2il7; hfbr2_2kl4; hfbr2_2kl9; hfbr2_3cl8; hfbr2_3fl6; hfbr2_3g8; hfbr2_312; hfbr2_41ml5; hfbr2_62bll; hfbr2_62fl0; hfbr2_62119; hfbr2_62nl0; hfbr2_62ol7; hfbr2_64all; hfbr2_64al5; hfbr2_64cl6; hfbr2_64c4; hfbr2_64h6; hfbr2_64i20; hfbr2_64jl8; hfbr2_64k24; hfbr2_64ol6; hfbr2_6al7; hfbr2_6b24; hfbr2_6i20; hfbr2_6ol7; hfbr2_71o20; hfbr2_72bl8; hfbr2_72dl3; hfbr2_72112; hfbr2_72ml6; hfbr2_72nl2; hfbr2_78c24; hfbr2_78dl3; hfbr2_78k24; hfbr2_78n23; hfbr2_7a24; hfbr2_7e22; hfbr2_7j4; hfbr2_82c20; hfbrl_10c20; hfbr2_82el7; hfbrl_10el7; hfbr2_82e4; hfbrl_10e4; hfbr2_82gl4; hfbrl_10gl4; hfbr2_82il7; hfbrl lO; hfbr2_82i24; hfbrl lO; hfbr2_82ml6; hfbrl lO; hfbr2_82m6; hfbrl lO; their complements; and variants thereof.

3. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_16f21; hfbr2_16k22; hfbr2_22f21; hfbr2_22hl3; hfbr2_22i4; hfbr2_22k3; hfbr2_22k8; hfbr2_23f2; ; hfbr2_23o24; hfbr2_23o5; hfbr2_2a2; hfbr2_2cl; hfbr2_2cl8; hfbr2_2d20; hibr2_2gl8; hfbr2_2hl; hfbr2_2hl0; hfbr2_2kl9; hfbr2_3fl6; hfbr2_312; hfbr2_62nl0; hfbr2_64al 1; hfbr2_64cl6; hfbr2_64c4; hfbr2_64h6; hfbr2_64i20; hfbr2_64ol6; hfbr2_6al7; hfbr2_6i20; hfbr2_71o20; hfbr2_72dl3; hfbr2_72ml6; hfbr2_72nl2; hfbr2_78dl3; hfbr2_78n23; hfbr2_7a24; hfbr2_7e22; hfbr2_7j4; hfbr2_82ml6; and hfbrl_10.

4. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfkd2_lj9; hfkd2_24al5; hfkd2_24bl5; hfkd2_24e23; hfkd2_24n20; hfkd2_24p5; hfkd2_3il3; hfkd2_3ol7; hfkd2_46a6; hfkd2_46bl0; hfkd2_46dl3; hfkd2_46j20; hfkd2_46kl9; hfkd2_46m4; hfkd2_47a4; hfkd2_4b6; hfkd2_4c8; hfkd2_4kl4; hfkd2_4mll; their complements; and variants thereof.

5. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfkd2_lj9; hfkd2_24e23; hfkd2_46a6; hfkd2_46bl0; hfkd2_46dl3; hfkd2_4b6; hfkd2_4c8; their complements; and variants thereof.

6. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hmcfl_lall; hmcfl_lc23; hmcfl_lel5; hmcfl_lgl3; their complements; and variants thereof.

7. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hmcfl_lc23 hmcfl_lgl3; their complements; and variants thereof.

8. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hhtes3_ln3; htes3_14g5; htes3_14h21; htes3_14pl4; htes3_14p7; htes3_15al3; Htes3_15c24; htes3_15c6; htes3_15gl4; htes3_15hl; htes3_15i5; htes3_15jl8; Htes3_15j3; htes3_15kll; htes3_17fl0; htes3_17117; htes3_17nl2; htes3_17nl8; Htes3_18f3; htes3_1817; htes3_19fl9; htes3_19jl7; htes3_lcl; htes3_lgl3; htes3_lkll; htes3_20c21; htes3_20k2; htes3_20ml8; htes3_21d4; htes3_21jl5; htes3_21116; htes3_21n23; htes3_22c23; htes3_22g2; htes3_22nl3; htes3_23111; htes3_23nl9; Htes3_23nl9; htes3_26g22; htes3_27dl; htes3_27k4; htes3_27ol4; htes3_28dl4; htes3_2al l; htes3_2al7; htes3_2dl5; htes3_2el2; htes3_2fl4; htes3_2g7; htes3_2hl; htes3_2hl5; htes3_2119; htes3_2ml8; htes3 2m20; htes3 2n9; htes3 2ol3; htes3 30f4; Htes3 35b4; htes3 35b5; htes3 35e21; htes3_35g6; htes3_35kl6; htes3_35k24; htes3_35nl2; htes3_35n24; htes3_35n9; htes3_35pl7; htes3_35p22; htes3_4b4; htes3_4fl7; htes3_4f5; htes3_4h6; htes3_4ol9; htes3_50j4; htes3_50n06; htes3_50n23; htes3_6b21; htes3_6cll; htes3_6dl6; htes3_72kll; Htes3_72kl5; htes3_72pl6; htes3_7b22; htes3_7dl7; htes3_7j3; htes3_7j8; htes3_7pl0; htes3_7p9; htes3_8e24; Htes3_8gll; Htes3_8g5; htes3_8ml0; Htes3_8p7; Htes3_9e22; Htes3_9i20; Htes3_9k22; their complements; and variants thereof.

9. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: htes3_14g5; htes3_14pl4; htes3_14p7; htes3_15al3; htes3_15gl4; htes3_15hl ; htes3_15jl8; htes3_17fl0; Htes3_18f3; htes3_19fl9; htes3_19jl7; htes3_20c21; htes3_21n23; htes3_22c23; htes3_22nl3; Htes3_23nl9; htes3_27ol4; htes3_28dl4; htes3_2al l; htes3_2dl5; htes3_2Ω4; htes3_2g7; htes3_2hl5; htes3_2119; htes3_2m20; htes3_2n9; htes3_30f4; htes3_35g6; htes3_35n24; htes3_35pl7; htes3_4b4; htes3_4Ω7; htes3_4ol9; htes3_50j4; htes3_50n23; htes3_50n06; htes3_6b21; htes3_6dl6; htes3_72kl 1; htes3_7dl7; htes3_7j8; Htes3_8gl 1; Htes3_8g5; Htes3_8p7; Htes3_9e22; Htes3_9i20; Htes3_9k22; their complements; and variants thereof.

10. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_16gl8; hfbr2_2kl4; Htes3_35b4; htes3_35p22; htes3_7j3; htes3_7pl0; hutel_20ml l; their complements; and variants thereof.

11. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_16cl6; hfbr2_2b5; htes3_15i5; htes3_1817; htes3_lkll; Htes3_72kl5; htes3_7b22; hutel_19g22; hutel_24j6; their complements; and variants thereof.

12. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_2dl5; htes3_35e21; hutel_2h3; their complements; and variants thereof.

13. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_23124; hfbr2_2il7; hfbr2_41ml5; hfbr2_62fl0; hfbr2_62119; hfbr2_64jl8; hfkd2_24n20; hfkd2_24p5; hfkd2_4kl4; htes3_lgl3; htes3_21116; htes3_23111; htes3_26g22; htes3_4h6; htes3_72pl6; hutel_19hl7; hutel_20hl3; hutel_24el l; their complements; and variants thereof.

14. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_3g8; hfbr2_62ol7; hfbr2_6b24; hfbr2_78k24; hfkd2_24bl5; hfkd2_3ol7; hfkd2_46j20; htes3_17117; htes3_17nl8; htes3_27dl; htes3_2al7; htes3_35b5; htes3_35kl6; htes3_35nl2; htes3_35n9; hutel_20bl9; hutel_20m24; hutel_23el3; their complements; and variants thereof.

15. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_23bl0; hfbr2_3cl8; hfbr2_64al5; hfbr2_6ol7; hfbr2_72bl8; hfbr2_72112; hfbr2_82i24(hfbrl_10)i htes3_14h21; Htes3_15j3; htes3_20ml8; htes3_22g2; htes3_2ml8; htes3_7p9; htes3_8ml0; hutel_1811; their complements; and variants thereof.

16. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_23b21; hfbr2_23nl6; hfbr2_2cl7; hfbr2_62bll; hfbr2_78c24; hfbr2_82e4 (hfbrl_10e4); hfbr2_82il7 (hfbrl_10); hfbr2_82m6 (hfbrl_10);_hfkd2_46m4; htes3_15kll; htes3_lcl; hhtes3_ln3; htes3_20k2; htes3_21d4; htes3_23nl9; htes3_4f5; htes3_6cll; htes3_8e24; hutel_20g21; hutel_22d2; hutel_22el2; their complements; and variants thereof.

17. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_16il2; hfbr2_ 16112; hfbr2_22hl3; hfbr2_2bl7; hfbr2_2dl7; hfbr2_64k24; hfbr2_82c20 (hfbrl_10c20); hfbr2_82el7 (hfbrl_10el7); hfbr2_82gl4 (hfbrl_10gl4); hfkd2_24al5; hfkd2_3il3; hfkd2_4mll; hmcfl_lall; hmcfl_lel5; htes3_15c6; htes3_2ol3; htes3_27k4; htes3_2hl; htes3_35k24; hutel_19fl9; and hutel_24cl9; their complements; and variants thereof.

18. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfkd2_46kl9; hfkd2_47a4; htes3_2el2; htes3_21jl5; htes3_17nl2; hutel_18il9; hutel_H2; their complements; and variants thereof.

19. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hutel_17k7; hutel_18cl2; hutel_18il9; hutel_18i4; hutel_1811; hutel_19fl9; hutel_19gl9; hutel_19g22; hutel_19hl7; hutel_19jll; hutel_li2; hutel_20bl9; hutel_20g21; hutel_20hl3; hutel_20mll; hutel_20m24; hutel_21dl5; hutel_22d2; hutel_22el2; hutel_22n2; hutel_22o2; hutel_23el3; hutel_23gll; hutel_24cl9; hutel_24ell; hutel_24j6; hutel_2h3; their complements; and variants thereof.

20. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hutel_17k7; hutel_18cl2; hutel_18i4; hutel_19gl9; hutel_19jl l; hutel _22n2; hutel_21dl5; hutel_22o2; hutel_23gl l ; their complements; and variants thereof.

21. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_16cl6; hfbr2_16f21; hfbr2_16gl8; hfbr2_16il2; hfbr2_16k22; hfbr2_16112; hfbr2_22f21; hfbr2_22hl3; hfbr2_22hl3; hfbr2_22i4; hfbr2_22k3; hfbr2_22k8; hfbr2_23bl0; hfbr2_23b21; hfbr2_23f2; hfbr2_23124; ; hfbr2_23nl6; hfbr2_23o24; hfbr2_23o5; hfbr2_2a2; hfbr2_2bl7; hfbr2_2b5; hfbr2_2cl; hfbr2_2cl7; hfbr2_2cl8; hfbr2_2dl5; hfbr2_2dl7; hfbr2_2d20; hfbr2_2gl8; hfbr2_2hl; hfbr2_2hl0; hfbr2_2il7; hfbr2_2kl4; hfbr2_2kl9; hfbr2_3cl8; hfbr2_3fl6; hfbr2_3g8; hfbr2_312; hfbr2_41ml5; hfbr2_62bl l; hfbr2_62Ω0; hfbr2_62119; hfbr2_62nl0; hfbr2_62ol7; hfbr2_64al l; hfbr2_64al5; hfbr2_64cl6; hfbr2_64c4; hfbr2_64h6; hfbr2_64i20; hfbr2_64jl8; hfbr2_64k24; hfbr2_64ol6; hfbr2_6al7; hfbr2_6b24; hfbr2_6i20; hfbr2_6ol7; hfbr2_71o20; hfbr2_72bl8; hfbr2_72dl3; hfbr2_72112; hfbr2_72ml6; hfbr2_72nl2; hfbr2_78c24; hfbr2_78dl3; hfbr2_78k24; hfbr2_78n23; hfbr2_7a24; hfbr2_7e22; hfbr2_7j4; hfbr2_82c20; hfbrl_10c20; hfbr2_82el7; hfbrl_10el7; hfbr2_82e4;; hfbrl_10e4; hfbr2_82gl4;; hfbrl_10gl4; hfbr2_82il7;; hfbrl lO; hfbr2_82i24;; hfbrl_10; hfbr2_82ml6;; hfbrl_10; hfbr2_82m6;; hfbrl_10; hfkd2_lj9; hfkd2_24al5; hfkd2_24bl5; hfkd2_24e23; hfkd2_24n20; hfkd2_24p5; hfkd2_3il3; hfkd2_3ol7; hfkd2_46a6; hfkd2_46bl0; hfkd2_46dl3; hfkd2_46j20; hfkd2_46kl9; hfkd2_46m4; hfkd2_47a4; hfkd2_4b6; hfkd2_4c8; hfkd2_4kl4; hfkd2_4mll; hmcfl lall; hmcfl_lc23; hmcfl_lel5; hmcfl_lgl3; hhtes3_ln3; htes3_14g5; htes3_14h21; htes3_14pl4; htes3_14p7; htes3_15al3; Htes3_15c24; htes3_15c6; htes3_15gl4; htes3_15hl; htes3_15i5; htes3_15jl8; Htes3_15j3; htes3_15kll; htes3_17fl0; htes3_17117; htes3_17nl2; htes3_17nl8; Htes3_18f3; htes3_1817; htes3_19fl9; htes3_19jl7; htes3_lcl; htes3_lgl3; htes3_lkl l; htes3_20c21; htes3_20k2; htes3_20ml8; htes3_21d4; htes3_21jl5; htes3_21116; htes3_21n23; htes3_22c23; htes3_22g2; htes3_22nl3; htes3_23111; htes3_23nl9; Htes3_23nl9; htes3_26g22; htes3_27dl; htes3_27k4; htes3_27ol4; htes3_28dl4; htes3_2all; htes3_2al7; htes3_2dl5; htes3_2el2; htes3_2fl4; htes3_2g7; htes3_2hl; htes3_2hl5; htes3_2119; htes3_2ml8; htes3_2m20; htes3_2n9; htes3_2ol3; htes3_30f4; Htes3_35b4; htes3_35b5; htes3_35e21 ; htes3_35g6; htes3_35kl6; htes3_35k24; htes3_35nl2; htes3_35n24; htes3_35n9; htes3_35pl7; htes3_35p22; htes3_4b4; htes3_4fl7; htes3_4f5; htes3_4h6; htes3_4ol9; htes3_50j4; htes3_50n06; htes3_50n23; htes3_6b21; htes3_6cll; htes3_6dl6; htes3_72kl l; Htes3_72kl5; htes3_72pl6; htes3_7b22; htes3_7dl7; htes3_7j3; htes3_7j8; htes3_7pl0; htes3_7p9; htes3_8e24; Htes3_8gll; Htes3_8g5; htes3_8mlO; Htes3_8ρ7; Htes3_9e22; Htes3_9i20; Htes3_9k22; hutel_17k7; hutel_18cl2; hutel_18il9; hutel_18i4; hutel lδll; hutel_19fl9; hutel_19gl9; hutel_19g22; hutel_19hl7; hutel_19jll; hutel_H2; hutel_20bl9; hutel_20g21; hutel_20hl3; hutel_20mll; hutel_20m24; hutel_21dl5; hutel_22d2; hutel_22el2; hutel_22n2; hutel_22o2; hutel_23el3; hutel_23gll; hutel_24cl9; hutel_24ell; hutel_24j6; hutel_2h3; their complements; and variants thereof.

22. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_16cl6; hfbr2_16f21; hfbr2_16gl8; hfbr2_16il2; hfbr2_16k22; hfbr2_16112; hfbr2_22f21; hfbr2_22hl3; hfbr2_22hl3; hfbr2_22i4; hfbr2_22k3; hfbr2_22k8; hfbr2_23bl0; hfbr2_23b21; hfbr2_23f2; hfbr2_23124; ; hfbr2_23nl6; hfbr2_23o24; hfbr2_23o5; hfbr2_2a2; hfbr2_2bl7; hfbr2_2b5; hfbr2_2cl; hfbr2_2cl7; hfbr2_2cl8; hfbr2_2dl5; hfbr2_2dl7; hfbr2_2d20; hfbr2_2gl8; hfbr2_2hl; hfbr2_2hl0; hfbr2_2il7; hfbr2_2kl4; hfbr2_2kl9; hfbr2_3cl8; hfbr2_3fl6; hfbr2_3g8; hfbr2_312; hfbr2_41ml5; hfbr2 62bll; hfbr2 62fl0; hfbr2 62119; hfbr2 62nl0; hfbr2 62ol7; hfbr2 64all; hfbr2_64al5; hfbr2_64cl6; hfbr2_64c4; hfbr2_64h6; hfbr2_64i20; hfbr2_64jl8; hfbr2_64k24; hfbr2_64ol6; hfbr2_6al7; hfbr2_6b24; hfbr2_6i20; hfbr2_6ol7; hfbr2_71o20; hfbr2_72bl8; hfbr2_72dl3; hfbr2_72112; hfbr2_72ml6; hfbr2_72nl2; hfbr2_78c24; hfbr2_78dl3; hfbr2_78k24; hfbr2_78n23; hfbr2_7a24; hfbr2_7e22; hfbr2_7j4; hfbr2_82c20; hfbrl_10c20; hfbr2_82el7; hfbrl_10el7; hfbr2_82e4; hfbrl_10e4; hfbr2_82gl4; hfbrl_10gl4; hfbr2_82il7; hfbrl lO; hfbr2_82i24; hfbrl_10; hfbr2_82ml6; hfbrl lO; hfbr2_82m6; hfbrl lO; complements of the nucleic acid sequences; and variants thereof.

23. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbι2_16£21; hfbr2_16k22; hfbr2_22f21; hfbr2_22hl3; hfbr2_22i4; hfbr2_22k3; hfbr2_22k8; hfbr2_23f2; ; hfbr2_23o24; hfbr2_23o5; hfbr2_2a2; hfbr2_2cl; hfbr2_2cl8; hfbr2_2d20; hfbr2_2gl8; hfbι2_2hl; hfbr2_2hl0; hfbr2_2kl9; hfbr2_3fl6; hfbr2_312; hfbr2_62nl0; hfbι2_64al 1; hfbr2_64cl6; hfbr2_64c4; hfbr2_64h6; hfbr2_64i20; hfbr2_64k24; hfbr2_64ol6; hfbr2_6al7; hfbr2_6i20; hfbr2_71o20; hfbr2_72dl3; hfbr2_72ml6; hfbr2_72nl2; hfbr2_78dl3; hfbr2_78n23; hfbr2_7a24; hfbr2_7e22; hfbr2_7j4; hfbr2_82ml6; hfbrl lO; complements of the nucleic acid sequences; and variants thereof.

24. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfkd2_lj9; hfkd2_24al5; hfkd2_24bl5; hfkd2_24e23; hfkd2_24n20; hfkd2_24p5; hfkd2_3il3; hfkd2_3ol7; hfkd2_46a6; hfkd2_46bl0; hfkd2_46dl3; hfkd2_46j20; hfkd2_46kl9; hfkd2_46m4; hfkd2_47a4; hfkd2_4b6; hfkd2_4c8; hfkd2_4kl4; hfkd2_4mll; complements of the nucleic acid sequences; and variants thereof.

25. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfkd2_lj9; hfkd2_24e23; hfkd2_46a6; hfkd2_46bl0; hfkd2_46dl3; hfkd2_4b6; hfkd2_4c8; complements of the nucleic acid sequences; and variants thereof.

26. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hmcfl_lall; hmcfl_lc23; hmcfl_lel5; hmcfl_lgl3; complements of the nucleic acid sequences; and variants thereof.

27. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hmcfl_lc23; hmcfl_lgl3; complements of the nucleic acid sequences; and variants thereof.

28. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hhtes3_ln3; htes3_14g5; htes3_14h21; htes3_14pl4; htes3_14ρ7; htes3_15al3; Htes3_15c24; htes3_15c6; htes3_15gl4; htes3_15hl; htes3_15i5; htes3_15jl8; Htes3_15j3; htes3_15kl l; htes3_17fl0; htes3_17117; htes3_17nl2; htes3_17nl8; Htes3_18f3; htes3_1817; htes3_19fl9; htes3_19jl7; htes3_lcl ; htes3_lgl3; htes3_lkll; htes3_20c21; htes3_20k2; htes3_20ml8; htes3_21d4; htes3_21jl5; htes3_21116; htes3_21n23; htes3_22c23; htes3_22g2; htes3_22nl3; htes3_23111; htes3_23nl9; Htes3_23nl9; htes3_26g22; htes3_27dl; htes3_27k4; htes3_27ol4; htes3_28dl4; htes3_2all; htes3_2al7; htes3_2dl5; htes3_2el2; htes3_2fl4; htes3_2g7; htes3_2hl; htes3_2hl5; htes3_2119; htes3_2ml8; htes3_2m20; htes3_2n9; htes3_2ol3; htes3_30f4; Htes3_35b4; htes3_35b5; htes3_35e21; htes3_35g6; htes3_35kl6; htes3_35k24; htes3_35nl2; htes3_35n24; htes3_35n9; htes3_35pl7; htes3_35p22; htes3_4b4; htes3_4fl7; htes3_4f5; htes3_4h6; htes3_4ol9; htes3_50j4; htes3_50n06; htes3_50n23; htes3_6b21; htes3_6cl l; htes3_6dl6; htes3_72kl l; Htes3_72kl5; htes3_72pl6; htes3_7b22; htes3_7dl7; htes3_7j3; htes3_7j8; htes3_7pl0; htes3_7p9; htes3_8e24; Htes3_8gll; Htes3_8g5; htes3_8mlO; Htes3_8p7; Htes3_9e22; Htes3_9i20; Htes3_9k22; complements of the nucleic acid sequences; and variants thereof.

29. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: htes3_14g5; htes3_14pl4; htes3_14p7; htes3_15al3; htes3_15gl4; htes3_15hl; htes3_15jl8; htes3_17fl0; htes3_17nl8; Htes3_18D; htes3_19Ω9; htes3_19jl7; htes3_20c21; htes3_21n23; htes3_22c23; htes3_22nl3; Htes3_23nl9; htes3_27ol4; htes3_28dl4; htes3_2al l; htes3_2dl5; htes3_2Ω4; htes3_2g7; htes3_2hl5; htes3_2119; htes3_2m20; htes3_2n9; htes3_30f4; htes3_35g6; htes3_35n24; htes3_35pl7; htes3_4b4; htes3_4fl7; htes3_4ol9; htes3_50j4; htes3_50n23; htes3_50n06; htes3_6b21; htes3_6dl6; htes3_72kl l; htes3_7dl7; htes3_7j8; Htes3_8gl 1 ; Htes3_8g5; Htes3_8p7; Htes3_9e22; Htes3_9i20; Htes3_9k22; complements of the nucleic acid sequences; and variants thereof.

30. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_16gl8; hfbr2_2kl4; Htes3_35b4; htes3_35p22; htes3_7j3; htes3_7pl0; hutel_20mll; complements of the nucleic acid sequences; and variants thereof.

31. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_16cl6; hfbr2_2b5; htes3_15i5; htes3_1817; htes3_lkll; Htes3_72kl5; htes3_7b22; hutel_19g22; hutel_24j6; complements of the nucleic acid sequences; and variants thereof.

32. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_2dl5; htes3_35e21; hutel_2h3; complements of the nucleic acid sequences; and variants thereof.

33. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_23124; hfbr2_2il7; hfbr2_41ml5; hfbr2_62fl0; hfbr2_62119; hfbr2_64jl8; hfkd2_24n20; hfkd2_24p5; hfkd2_4kl4; htes3_lgl3; htes3_21116; htes3_23111; htes3_26g22; htes3_4h6; htes3_72pl6; hutel_19hl7; hutel_20hl3; hutel_24ell; complements of the nucleic acid sequences; and variants thereof.

34. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_3g8; hfbr2_62ol7; hfbr2_6b24; hfbr2_78k24; hfkd2_24bl5; hfkd2_3ol7; hfkd2_46j20; htes3_17117; Htes3_17nl8; htes3_27dl; htes3_2al7; htes3_35b5; htes3_35kl6; htes3_35nl2; htes3_35n9; hutel_20bl9; hutel_20m24; hutel_23el3; complements of the nucleic acid sequences; and variants thereof.

35. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_23bl0; hfbr2_3cl8; hfbr2_64al5; hfbr2_6ol7; hfbr2_72bl8; hfbr2_72112; hfbr2_82i24(hfbrl_10)i_htes3_14h21; Htes3_15j3; htes3_20ml8; htes3_22g2; htes3_2ml8; htes3_7p9; htes3_8ml0; hutel_1811; complements of the nucleic acid sequences; and variants thereof.

36. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_23b21; hfbr2_23nl6; hfbr2_2cl7; hfbr2_62bl l; hfbr2_78c24; hfbr2_82e4 (hfbrl_10e4); hfbr2_82il7 (hfbrl_10); hfbr2_82m6 (hfbrl_10);_hfkd2_46m4; htes3_15kll; htes3_lcl; hhtes3_ln3; htes3_20k2; htes3_21d4; htes3_23nl9; htes3_4f5; htes3_6cl l; htes3_8e24; hutel_20g21; hutel_22d2; hutel_22el2; complements of the nucleic acid sequences; and variants thereof.

37. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_16il2; hfbr2_16112; hfbr2_22hl3; hfbr2_2bl7; hfbr2_2dl7; hfbr2_64k24; hfbr2_82c20 (hfbrl_10c20);_hfbr2_82el7 (hfbrl_10el7); hfbr2_82gl4 (hfbrl_10gl4); hfkd2_24al5; hfkd2_3il3; hfkd2_4mll; hmcfl_lall; hmcfl_lel5; htes3_15c6; htes3_2ol3; htes3_27k4; htes3_2hl; htes3_35k24; hutel_19fl9; and hutel_24cl9; complements of the nucleic acid sequences; and variants thereof.

38. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfkd2_46kl9; hfkd2_47a4; htes3_2el2; htes3_21jl5; htes3_17nl2; hutel_18il9; hutel_li2; complements of the nucleic acid sequences; and variants thereof.

39. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hutel_17k7; hutel_18cl2; hutel_18il9; hutel_18i4; hutel_1811; hutel_19fl9; hutel_19gl9; hutel_19g22; hutel_19hl7; hutel_19jll; hutel_li2; hutel_20bl9; hutel_20g21; hutel_20hl3; hutel_20ml l; hutel_20m24; hutel_21dl5; hutel_22d2; hutel_22el2; hutel_22n2; hutel_22o2; hutel_23el3; hutel_23gll; hutel_24cl9; hutel_24ell; hutel_24j6; hutel_2h3; complements of the nucleic acid sequences; and variants thereof.

40. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hutel_17k7; hutel_18cl2; hutel_18i4; hutel_19gl9; hutel_19j l l ; hutel _22n2; hutel_21dl5; hutel_22o2; hutel_23gl l ; complements of the nucleic acid sequences; and variants thereof.

41. A nucleic acid molecule having the sequence of a clone selected from the group consisting of hfbr2_16cl6; hfbr2_16f21; hfbr2_16gl8; hfbr2_16il2; hfbr2_16k22; hfbr2_16112; hfbr2_22f21; hfbr2_22hl3; hfbr2_22hl3; hfbr2_22i4; hfbr2_22k3; hfbr2_22k8; hfbr2_23bl0; hfbr2_23b21; hfbr2_23f2; hfbr2_23124; hfbr2_23nl6; hfbr2_23o24; hfbr2_23o5; hfbr2_2a2; hfbr2_2bl7; hfbr2_2b5; hfbr2_2cl; hfbr2_2cl7; hfbr2_2cl8; hfbr2_2dl5; hfbr2_2dl7; hfbr2_2d20; hfbr2_2gl8; hfbr2_2hl; hfbr2_2hl0; hfbr2_2il7; hfbr2_2kl4; hfbr2_2kl9; hfbr2_3bl6; hfbr2_3cl8; hfbr2_3fl6; hfbr2_3g8; hfbr2_312; hfbr2_41ml5; hfbr2_62bll; hfbr2_62fl0; hfbr2_62119; hfbr2_62nl0; hfbr2_62ol7; hfbr2_64all; hfbr2_64al5; hfbr2_64cl6; hfbr2_64c4; hfbr2_64h6; hfbr2_64i20; hfbr2_64jl8; hfbr2_64k24; hfbr2_64ol6; hfbr2_6al7; hfbr2_6b24; hfbr2_6i20; hfbr2_6ol7; hfbr2_71o20; hfbr2_72bl8; hfbr2_72dl3; hfbr2_72112; hfbr2_72ml6; hfbr2_72nl2; hfbr2_78c24; hfbr2_78dl3; hfbr2_78k24; hfbr2_78n23; hfbr2_7a24; hfbr2_7e22; hfbr2_7j4; hfbr2_82c20; hfbrl_10c20; hfbr2_82el7; hfbrl_10el7; hfbr2_82e4;; hfbrl_10e4; hfbr2_82gl4;; hfbrl_10gl4; hfbr2_82il7;; hfbrl_10; hfbr2_82i24;; hfbrl lO; hfbr2_82ml6;; hfbrl_10; hfbr2_82m6;; hfbrl_10; hfkd2_lj9; hfkd2_24al5; hfkd2_24bl5; hfkd2_24e23; hfkd2_24n20; hfkd2_24p5; hfkd2_3il3; hfkd2_3ol7; hfkd2_46a6; hfkd2_46bl0; hfkd2_46dl3; hfkd2_46j20; hfkd2_46kl9; hfkd2_46m4; hfkd2_47a4; hfkd2_4b6; hfkd2_4c8; hfkd2_4kl4; hfkd2_4ml l; hmcfl lall; hmcfl_lc23; hmcfl_lel5; hmcfl_lgl3; hhtes3_ln3; htes3_14g5; htes3_14h21; htes3_14pl4; htes3_14p7; htes3_15al3; Htes3_15c24; htes3_15c6; htes3_15gl4; htes3_15hl; htes3_15i5; htes3_15jl8; Htes3_15j3; htes3_15kll; htes3_17fl0; htes3_17117; htes3_17nl2; htes3_17nl8; Htes3_18f3; htes3_1817; htes3_19fl9; htes3_19jl7; htes3_lcl; htes3_lgl3; htes3_lkll; htes3_20c21; htes3_20k2; htes3_20ml8; htes3_21d4; htes3_21jl5; htes3_21116; htes3_21n23; htes3_22c23; htes3_22g2; htes3_22nl3; htes3_23111; htes3_23nl9; Htes3_23nl9; htes3_26g22; htes3_27dl; htes3_27k4; htes3_27ol4; htes3_28dl4; htes3_2all; htes3_2al7; htes3_2dl5; htes3_2el2; htes3_2fl4; htes3_2g7; htes3_2hl; htes3_2hl5; htes3_2119; htes3_2ml8; htes3_2m20; htes3_2n9; htes3_2ol3; htes3_30f4; Htes3_35b4; htes3_35b5; htes3_35e21; htes3_35g6; htes3_35kl6; htes3_35k24; htes3_35nl2; htes3_35n24; htes3_35n9; htes3_35pl7; htes3_35p22; htes3_4b4; htes3_4fl7; htes3_4f5; htes3_4h6; htes3_4ol9; htes3_50j4; htes3_50n06; htes3_50n23; htes3_6b21; htes3_6cl l; htes3_6dl6; htes3_72kll; Htes3_72kl5; htes3_72pl6; htes3_7b22; htes3_7dl7; htes3_7j3; htes3_7j8; htes3_7pl0; htes3_7p9; htes3_8e24; Htes3_8gll; Htes3_8g5; htes3_8ml0; Htes3_8p7; Htes3_9e22; Htes3_9i20; Htes3_9k22; hutel_17k7; hutel_18cl2; hutel_18il9; hutel_18i4; hutel_1811; hutel_19fl9; hutel_19gl9; hutel_19g22; hutel_19hl7; hutel_19jl l; hutel_li2; hutel_20bl9; hutel_20g21; hutel_20hl3; hutel_20mll; hutel_20m24; hutel_21dl5; hutel_22d2; hutel_22el2; hutel_22n2; hutel_22o2; hutel_23el3; hutel_23gll; hutel_24cl9; hutel_24ell; hutel_24j6; hutel_2h3; their complements; and variants thereof.

42. A polypeptide encoded by the nucleic acid molecule according to claim 41.

43. An antibody or fragment thereof that is capable of binding to a specific portion of the peptide according to claim 42.

44. A pharmaceutical composition, comprising (a) an effective amount of a pharmaceutical agent, wherein said pharmaceutical agent is selected from the group consisting of the polypeptide according to claim 42, variants or functional derivatives thereof, and antibodies thereto; and (2) a physiologically acceptable carrier or excipient.

45. An expression vector comprising the nucleic acid molecule of claim 41 or a fragment thereof, and optionally a promoter operably linked to said nucleic acid molecule or said fragment.

46. A method for recombinantly producing a desired peptide, comprising expressing in a host cell a peptide encoded by the nucleic acid molecule according to claim 41.