JP4194934B2 - カプセル化細胞によるタンパク質の分泌法 - Google Patents

カプセル化細胞によるタンパク質の分泌法 Download PDF

Info

Publication number
JP4194934B2
JP4194934B2 JP2003500287A JP2003500287A JP4194934B2 JP 4194934 B2 JP4194934 B2 JP 4194934B2 JP 2003500287 A JP2003500287 A JP 2003500287A JP 2003500287 A JP2003500287 A JP 2003500287A JP 4194934 B2 JP4194934 B2 JP 4194934B2
Authority
JP
Japan
Prior art keywords
cells
cell
molecule
secreted
molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2003500287A
Other languages
English (en)
Japanese (ja)
Other versions
JP2004528574A5 (https=
JP2004528574A (ja
Inventor
ダイアナ ジェスパーセン
エフゲーニャ アクセルバンド
パトリシア マクグラス
ヤン トルノフスキ
フィリップ ティー． ジュニア モーン
Original Assignee
ワン セル システムズ インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ワン セル システムズ インコーポレイテッド filed Critical ワン セル システムズ インコーポレイテッド
Publication of JP2004528574A publication Critical patent/JP2004528574A/ja
Publication of JP2004528574A5 publication Critical patent/JP2004528574A5/ja
Application granted granted Critical
Publication of JP4194934B2 publication Critical patent/JP4194934B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/5432—Liposomes or microcapsules
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/5436—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand physically entrapped within the solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/823—Immunogenic carrier or carrier per se

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2003500287A 2001-05-26 2002-05-28 カプセル化細胞によるタンパク質の分泌法 Expired - Fee Related JP4194934B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29382201P 2001-05-26 2001-05-26
PCT/US2002/016783 WO2002097122A1 (en) 2001-05-26 2002-05-28 Secretion of proteins by encapsulated cells

Publications (3)

Publication Number Publication Date
JP2004528574A JP2004528574A (ja) 2004-09-16
JP2004528574A5 JP2004528574A5 (https=) 2005-12-22
JP4194934B2 true JP4194934B2 (ja) 2008-12-10

Family

ID=23130737

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2003500287A Expired - Fee Related JP4194934B2 (ja) 2001-05-26 2002-05-28 カプセル化細胞によるタンパク質の分泌法

Country Status (8)

Country Link
US (2) US6806058B2 (https=)
EP (1) EP1399580B1 (https=)
JP (1) JP4194934B2 (https=)
AT (1) ATE410680T1 (https=)
AU (1) AU2002314820B2 (https=)
CA (1) CA2447691A1 (https=)
DE (1) DE60229246D1 (https=)
WO (1) WO2002097122A1 (https=)

Families Citing this family (137)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
AU2004229440B2 (en) 2003-04-10 2010-08-12 President And Fellows Of Harvard College Formation and control of fluidic species
WO2005021151A1 (en) 2003-08-27 2005-03-10 President And Fellows Of Harvard College Electronic control of fluidic species
CA2539452C (en) * 2003-09-17 2013-10-29 Guava Technologies, Inc. Compositions and methods for analysis of target analytes
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7655470B2 (en) 2004-10-29 2010-02-02 University Of Chicago Method for manipulating a plurality of plugs and performing reactions therein in microfluidic systems
US9477233B2 (en) 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2006096571A2 (en) 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
US20070054119A1 (en) * 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2530167A1 (en) 2006-05-11 2012-12-05 Raindance Technologies, Inc. Microfluidic Devices
EP2077912B1 (en) 2006-08-07 2019-03-27 The President and Fellows of Harvard College Fluorocarbon emulsion stabilizing surfactants
WO2008073166A2 (en) * 2006-08-23 2008-06-19 Xcellerex, Inc. Multi-variant cell indication technique
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
EP2136786B8 (en) * 2007-03-28 2012-11-14 President and Fellows of Harvard College Apparatus for forming droplets
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
WO2009011808A1 (en) * 2007-07-13 2009-01-22 President And Fellows Of Harvard College Droplet-based selection
WO2009026448A1 (en) * 2007-08-21 2009-02-26 Affomix Corporation Interaction screening methods, systems and devices
JP2010537643A (ja) * 2007-08-29 2010-12-09 アプライド バイオシステムズ， エルエルシー 代替的な核酸配列決定法
JP5738597B2 (ja) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸の配列決定のためのシステムおよび方法
US7842475B2 (en) * 2008-01-08 2010-11-30 Siemens Healthcare Diagnostics Inc. Stabilization of solid support assay reagents
WO2009111014A2 (en) * 2008-03-04 2009-09-11 Crystal Bioscience Inc. Gel microdrop composition and method of using the same
WO2010005593A1 (en) * 2008-07-11 2010-01-14 President And Fellows Of Harvard College Systems and methods of droplet-based selection
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
WO2010033200A2 (en) * 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3150724A1 (en) 2008-12-19 2017-04-05 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
KR101893613B1 (ko) 2009-04-13 2018-08-30 유니버시티 오브 워싱톤 스루 이츠 센터 포 커머셜리제이션 앙상블 결정 부분 표본 순위화
WO2011028764A2 (en) 2009-09-02 2011-03-10 President And Fellows Of Harvard College Multiple emulsions created using jetting and other techniques
US10520500B2 (en) 2009-10-09 2019-12-31 Abdeslam El Harrak Labelled silica-based nanomaterial with enhanced properties and uses thereof
JP5791621B2 (ja) 2009-10-27 2015-10-07 プレジデント アンド フェローズ オブ ハーバード カレッジ 液滴生成技術
US10837883B2 (en) 2009-12-23 2020-11-17 Bio-Rad Laboratories, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
EP4484577A3 (en) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9562897B2 (en) 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
WO2012079749A1 (en) 2010-12-16 2012-06-21 Stichting Dutch Polymer Institute Process for making cell suspension
EP3412778A1 (en) 2011-02-11 2018-12-12 Raindance Technologies, Inc. Methods for forming mixed droplets
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
BR112013029729A2 (pt) 2011-05-23 2017-01-24 Basf Se controle de emulsões, incluindo emulsões múltiplas
EP3216872B1 (en) 2011-06-02 2020-04-01 Bio-Rad Laboratories, Inc. Enzyme quantification
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
CN103764265A (zh) 2011-07-06 2014-04-30 哈佛学院院长等 多重乳剂和用于配制多重乳剂的技术
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US20150177115A1 (en) 2012-04-06 2015-06-25 Slingshot Biosciences Hydrogel particles with tunable optical properties
WO2013155525A1 (en) * 2012-04-13 2013-10-17 Biolumix, Inc Ultra rapid blood culturing and susceptibility testing system
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2708890B1 (en) 2012-09-14 2015-11-18 Molecular Devices (New Milton) Ltd Method of selecting a monoclonal cell colony
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20200140929A (ko) 2013-02-08 2020-12-16 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
HK1220759A1 (zh) 2013-03-15 2017-05-12 Hycor Biomedical Llc 用於进行过敏症和自身免疫性疾病的诊断测定的自动化免疫分析系统
US10527626B2 (en) 2013-07-05 2020-01-07 University Of Washington Through Its Center For Commercialization Methods, compositions and systems for microfluidic assays
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
WO2015103367A1 (en) 2013-12-31 2015-07-09 Raindance Technologies, Inc. System and method for detection of rna species
GB201402591D0 (en) 2014-02-14 2014-04-02 Memo Therapeutics Ag Method for recovering two or more genes, or gene products, encoding an immunoreceptor
BR112016023625A2 (pt) 2014-04-10 2018-06-26 10X Genomics, Inc. dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2015200893A2 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
KR20170023979A (ko) 2014-06-26 2017-03-06 10엑스 제노믹스, 인크. 핵산 서열 조립을 위한 프로세스 및 시스템
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
BR112017008877A2 (pt) 2014-10-29 2018-07-03 10X Genomics Inc métodos e composições para sequenciamento de ácido nucleico-alvo
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
JP6769969B2 (ja) 2015-01-12 2020-10-14 １０エックス ジェノミクス， インコーポレイテッド 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー
WO2016115273A1 (en) 2015-01-13 2016-07-21 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
JP6692834B2 (ja) 2015-02-09 2020-05-13 スリングショット バイオサイエンシーズ， インコーポレイテッド 調整可能な光学特性を有するヒドロゲル粒子およびその使用の方法
WO2016130578A1 (en) 2015-02-09 2016-08-18 10X Genomics, Inc. Systems and methods for determining structural variation and phasing using variant call data
MX2017010857A (es) 2015-02-24 2017-12-11 10X Genomics Inc Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana.
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
FR3033333A1 (fr) * 2015-03-06 2016-09-09 Commissariat Energie Atomique Procede et dispositif pour detecter en temps reel un compose secrete et la cible secretrice ainsi que leurs utilisations
WO2016145242A1 (en) * 2015-03-11 2016-09-15 Agenus Inc. Methods and compositions for high throughput screening of biomolecules using gel microdrops
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
JP6785465B2 (ja) * 2015-10-02 2020-11-18 国立大学法人広島大学 微生物の分離培養方法及び凝集体含有溶液
JP2018537414A (ja) 2015-10-13 2018-12-20 プレジデント アンド フェローズ オブ ハーバード カレッジ ゲルマイクロスフェアの作製及び使用のためのシステム及び方法
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
JP6954899B2 (ja) 2015-12-04 2021-10-27 １０エックス ゲノミクス，インコーポレイテッド 核酸の解析のための方法及び組成物
US20190031743A1 (en) * 2016-01-29 2019-01-31 Achaogen, Inc. Screening methods for identifying antibodies that bind cell surface epitopes
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2018049248A1 (en) 2016-09-09 2018-03-15 Icellhealth Consulting Llc Oncolytic virus equipped with bispecific engager molecules
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3343223A1 (en) 2016-12-30 2018-07-04 Blink AG A prefabricated microparticle for performing a digital detection of an analyte
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP3838268B1 (en) 2017-02-24 2023-05-10 The Regents of the University of California Particle-drop structures and methods for making and using the same
EP3615574A4 (en) 2017-04-26 2021-02-24 Eureka Therapeutics, Inc. SPECIFIC GLYPICAN 3 RECOGNIZING CONSTRUCTS AND THEIR USE
EP3625715A4 (en) 2017-05-19 2021-03-17 10X Genomics, Inc. DATA SET ANALYSIS SYSTEMS AND METHODS
EP3445876B1 (en) 2017-05-26 2023-07-05 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
WO2019176275A1 (ja) * 2018-03-12 2019-09-19 ソニー株式会社 細胞評価装置及び細胞評価システム
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
CN110498858B (zh) * 2019-07-26 2024-01-23 深圳市达科为生物工程有限公司 一种动态检测单细胞外泌蛋白分泌情况的方法
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
KR20220129585A (ko) 2020-01-24 2022-09-23 슬링샷 바이오사이언시즈 인코포레이티드 세포-유사 보정 입자를 위한 조성물 및 방법
CN115428088A (zh) 2020-02-13 2022-12-02 10X基因组学有限公司 用于基因表达和dna染色质可及性的联合交互式可视化的系统和方法
JP7807395B2 (ja) 2020-05-04 2026-01-27 スリングショット バイオサイエンシーズ， インコーポレイテッド 多重化アッセイのためのパッシブ光学バーコード付与のための組成物および方法
WO2022147480A1 (en) 2020-12-30 2022-07-07 Ansun Biopharma, Inc. Oncolytic virus encoding sialidase and multispecific immune cell engager
WO2022221391A1 (en) 2021-04-14 2022-10-20 Partillion Bioscience Corporation Nanoscale reaction chambers and methods of using the same
CN113917159A (zh) * 2021-09-30 2022-01-11 杭州联科生物技术股份有限公司 一种淋巴细胞亚群的流式检测方法
AU2022376541A1 (en) 2021-10-29 2024-04-18 Slingshot Biosciences, Inc. Hydrogel particles as feeder cells and as synthetic antigen presenting cells
US20240041939A1 (en) * 2021-12-17 2024-02-08 William Marsh Rice University Encapsulated cells expressing il-12 and uses thereof
CN119907921A (zh) 2022-05-05 2025-04-29 弹弓生物科学公司 用于血液学的作为红细胞模拟物的工程化颗粒和含有工程化颗粒的组合物
CA3257621A1 (en) 2022-06-02 2023-12-07 Slingshot Biosciences, Inc. APOPTOTIC CELL MIMETIC
CN120712108A (zh) 2022-10-26 2025-09-26 弹弓生物科学公司 具有可调整光学性质的大小可调整合成颗粒和用于使用其进行免疫细胞激活的方法
WO2025049609A1 (en) 2023-08-29 2025-03-06 Slingshot Biosciences, Inc. Cd34 stem cell mimics

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4801529A (en) 1985-06-18 1989-01-31 Brandeis University Methods for isolating mutant microoganisms using microcapsules coated with indicator material
US4959301A (en) 1988-04-22 1990-09-25 Massachusetts Institute Of Technology Process for rapidly enumerating viable entities
US5225332A (en) 1988-04-22 1993-07-06 Massachusetts Institute Of Technology Process for manipulation of non-aqueous surrounded microdroplets
US5888728A (en) 1988-10-17 1999-03-30 Molecular Devices Corporation Hapten derivatized capture membrane and diagnostic assays using such membrane
US5965379A (en) * 1991-07-19 1999-10-12 Cytimmune Sciences Inc. Method for measuring endogenous cytokines
US6309883B1 (en) * 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US20030215798A1 (en) * 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US6068979A (en) * 1998-04-22 2000-05-30 Lumigen, Inc. Simplified sequential chemiluminescent detection
GB9928193D0 (en) 1999-11-29 2000-01-26 Univ Bristol Cell treatment method
CA2392537A1 (en) 1999-12-03 2001-06-07 The Trustees Of The University Of Pennsylvania Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals

Also Published As

Publication number Publication date
US6806058B2 (en) 2004-10-19
US7556928B2 (en) 2009-07-07
US20050019839A1 (en) 2005-01-27
EP1399580B1 (en) 2008-10-08
CA2447691A1 (en) 2002-12-05
DE60229246D1 (https=) 2008-11-20
ATE410680T1 (de) 2008-10-15
JP2004528574A (ja) 2004-09-16
US20030143642A1 (en) 2003-07-31
AU2002314820B2 (en) 2008-01-24
EP1399580A1 (en) 2004-03-24
EP1399580A4 (en) 2005-06-08
WO2002097122A1 (en) 2002-12-05

Similar Documents

Publication Publication Date Title
JP4194934B2 (ja) カプセル化細胞によるタンパク質の分泌法
AU2002314820A1 (en) Secretion of Molecules by Encapsulated Cells
JP4450998B2 (ja) フローサイトメトリの全血樹状細胞免疫機能アッセイ
Powell et al. Gel microdroplets and flow cytometry: rapid determination of antibody secretion by individual cells within a cell population
EP2726879B1 (en) Requlatory t cells and methods of identifying and isolating them using cd6 -expression or the combination of cd4, cd25 and cd127
AU2014232225B2 (en) Protocol for identifying and isolating antigen-specific B cells and producing antibodies to desired antigens
Gray et al. Secretion capture and report web: use of affinity derivatized agarose microdroplets for the selection of hybridoma cells
Caraher et al. Flow cytometric analysis of intracellular IFN-γ, IL-4 and IL-10 in CD3+ 4+ T-cells from rat spleen
WO2009140876A1 (zh) 从生物体液样本中富集与检测稀有细胞的整合方法
WO2005012505A2 (en) Nanoparticle and microparticle based detection of cellular products
Zola Detection of cytokine receptors by flow cytometry
Tai et al. Isolation and characterization of human multiple myeloma cell enriched populations
US20190352607A1 (en) Methods for expanding and differentiating b cells for producing antibody
JP2025114771A (ja) マルチパラメータサイトメトリーに基づく白血球サブセット化の為の手段及び方法
WO2009103753A1 (en) Methods for identifying and/or sorting cells by secreted molecule and kits for performing such methods
EP2290368A1 (en) Quality assays for antigen presenting cells
Collins Cytokine and cytokine receptor expression as a biological indicator of immune activation: important considerations in the development of in vitro model systems
CN110178034A (zh) 用于从生物样品中测定免疫球蛋白γ(IgG)抗体同种型浓度的方法和试剂
US9063145B2 (en) Multivalent opsonophagocytic assay using bead array and imaging
Lanzavecchia et al. Human T cell lines with antigen specificity and helper activity
Chesneau et al. New method for the expansion of highly purified human regulatory granzyme B-expressing B cells
AU2003243273A1 (en) Detection of secreted polypeptides
Loza et al. Multiple color immunofluorescence for cytokine detection at the single-cell level
CN112921004B (zh) 用于cd3受体激动剂检测的细胞株及其构建方法及cd3受体激动剂检测方法
AU2021392748A1 (en) Methods of selectively targeting cd6 high cells and decreasing activity of t eff cells

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20050105

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20050105

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20071205

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20071214

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20080311

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20080318

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20080410

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20080417

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20080512

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20080610

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20080903

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20080924

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20111003

Year of fee payment: 3

LAPS Cancellation because of no payment of annual fees