JP4194934B2 - カプセル化細胞によるタンパク質の分泌法 - Google Patents
カプセル化細胞によるタンパク質の分泌法 Download PDFInfo
- Publication number
- JP4194934B2 JP4194934B2 JP2003500287A JP2003500287A JP4194934B2 JP 4194934 B2 JP4194934 B2 JP 4194934B2 JP 2003500287 A JP2003500287 A JP 2003500287A JP 2003500287 A JP2003500287 A JP 2003500287A JP 4194934 B2 JP4194934 B2 JP 4194934B2
- Authority
- JP
- Japan
- Prior art keywords
- cells
- cell
- molecule
- secreted
- molecules
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/5432—Liposomes or microcapsules
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/5436—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand physically entrapped within the solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
- G01N33/56972—White blood cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/52—Constant or Fc region; Isotype
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/823—Immunogenic carrier or carrier per se
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29382201P | 2001-05-26 | 2001-05-26 | |
| PCT/US2002/016783 WO2002097122A1 (en) | 2001-05-26 | 2002-05-28 | Secretion of proteins by encapsulated cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2004528574A JP2004528574A (ja) | 2004-09-16 |
| JP2004528574A5 JP2004528574A5 (enExample) | 2005-12-22 |
| JP4194934B2 true JP4194934B2 (ja) | 2008-12-10 |
Family
ID=23130737
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003500287A Expired - Fee Related JP4194934B2 (ja) | 2001-05-26 | 2002-05-28 | カプセル化細胞によるタンパク質の分泌法 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US6806058B2 (enExample) |
| EP (1) | EP1399580B1 (enExample) |
| JP (1) | JP4194934B2 (enExample) |
| AT (1) | ATE410680T1 (enExample) |
| AU (1) | AU2002314820B2 (enExample) |
| CA (1) | CA2447691A1 (enExample) |
| DE (1) | DE60229246D1 (enExample) |
| WO (1) | WO2002097122A1 (enExample) |
Families Citing this family (138)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006507921A (ja) | 2002-06-28 | 2006-03-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 流体分散のための方法および装置 |
| US20060078893A1 (en) | 2004-10-12 | 2006-04-13 | Medical Research Council | Compartmentalised combinatorial chemistry by microfluidic control |
| GB0307428D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Compartmentalised combinatorial chemistry |
| GB0307403D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Selection by compartmentalised screening |
| CA2521862C (en) | 2003-04-10 | 2012-10-16 | President And Fellows Of Harvard College | Formation and control of fluidic species |
| CN1842368B (zh) | 2003-08-27 | 2014-05-28 | 哈佛大学 | 流体物种的电子控制 |
| EP1664784A1 (en) * | 2003-09-17 | 2006-06-07 | Guava Technologies, Inc. | Compositions and methods for analysis of target analytes |
| US20050221339A1 (en) | 2004-03-31 | 2005-10-06 | Medical Research Council Harvard University | Compartmentalised screening by microfluidic control |
| US9477233B2 (en) | 2004-07-02 | 2016-10-25 | The University Of Chicago | Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets |
| US7655470B2 (en) | 2004-10-29 | 2010-02-02 | University Of Chicago | Method for manipulating a plurality of plugs and performing reactions therein in microfluidic systems |
| US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
| US20070054119A1 (en) * | 2005-03-04 | 2007-03-08 | Piotr Garstecki | Systems and methods of forming particles |
| US9039273B2 (en) | 2005-03-04 | 2015-05-26 | President And Fellows Of Harvard College | Method and apparatus for forming multiple emulsions |
| WO2007081385A2 (en) | 2006-01-11 | 2007-07-19 | Raindance Technologies, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
| US9562837B2 (en) | 2006-05-11 | 2017-02-07 | Raindance Technologies, Inc. | Systems for handling microfludic droplets |
| WO2007133710A2 (en) | 2006-05-11 | 2007-11-22 | Raindance Technologies, Inc. | Microfluidic devices and methods of use thereof |
| US9012390B2 (en) | 2006-08-07 | 2015-04-21 | Raindance Technologies, Inc. | Fluorocarbon emulsion stabilizing surfactants |
| WO2008073166A2 (en) * | 2006-08-23 | 2008-06-19 | Xcellerex, Inc. | Multi-variant cell indication technique |
| WO2008097559A2 (en) | 2007-02-06 | 2008-08-14 | Brandeis University | Manipulation of fluids and reactions in microfluidic systems |
| WO2008109176A2 (en) * | 2007-03-07 | 2008-09-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
| EP2136786B8 (en) * | 2007-03-28 | 2012-11-14 | President and Fellows of Harvard College | Apparatus for forming droplets |
| WO2008130623A1 (en) | 2007-04-19 | 2008-10-30 | Brandeis University | Manipulation of fluids, fluid components and reactions in microfluidic systems |
| US20090068170A1 (en) * | 2007-07-13 | 2009-03-12 | President And Fellows Of Harvard College | Droplet-based selection |
| WO2009026448A1 (en) * | 2007-08-21 | 2009-02-26 | Affomix Corporation | Interaction screening methods, systems and devices |
| EP2201021A4 (en) * | 2007-08-29 | 2012-01-25 | Applied Biosystems Llc | METHOD OF ALTERNATING NUCLEIC ACID SEQUENCING |
| JP5738597B2 (ja) | 2007-12-21 | 2015-06-24 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 核酸の配列決定のためのシステムおよび方法 |
| US7842475B2 (en) * | 2008-01-08 | 2010-11-30 | Siemens Healthcare Diagnostics Inc. | Stabilization of solid support assay reagents |
| JP5737707B2 (ja) * | 2008-03-04 | 2015-06-17 | クリスタル バイオサイエンス インク.Crystal Bioscience Inc. | ゲル状マイクロドロップ組成物およびその使用方法 |
| WO2010005593A1 (en) * | 2008-07-11 | 2010-01-14 | President And Fellows Of Harvard College | Systems and methods of droplet-based selection |
| EP4047367A1 (en) | 2008-07-18 | 2022-08-24 | Bio-Rad Laboratories, Inc. | Method for detecting target analytes with droplet libraries |
| US12038438B2 (en) | 2008-07-18 | 2024-07-16 | Bio-Rad Laboratories, Inc. | Enzyme quantification |
| US20110218123A1 (en) | 2008-09-19 | 2011-09-08 | President And Fellows Of Harvard College | Creation of libraries of droplets and related species |
| EP3290531B1 (en) | 2008-12-19 | 2019-07-24 | President and Fellows of Harvard College | Particle-assisted nucleic acid sequencing |
| WO2010111231A1 (en) | 2009-03-23 | 2010-09-30 | Raindance Technologies, Inc. | Manipulation of microfluidic droplets |
| ES2638861T3 (es) * | 2009-04-13 | 2017-10-24 | University Of Washington | Método y aparato de detección de partículas raras |
| EP2473263B1 (en) | 2009-09-02 | 2022-11-02 | President and Fellows of Harvard College | Multiple emulsions created using jetting and other techniques |
| US10520500B2 (en) | 2009-10-09 | 2019-12-31 | Abdeslam El Harrak | Labelled silica-based nanomaterial with enhanced properties and uses thereof |
| EP2493619B1 (en) | 2009-10-27 | 2018-12-19 | President and Fellows of Harvard College | Droplet creation techniques |
| EP2517025B1 (en) | 2009-12-23 | 2019-11-27 | Bio-Rad Laboratories, Inc. | Methods for reducing the exchange of molecules between droplets |
| EP4435111A1 (en) | 2010-02-12 | 2024-09-25 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
| US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
| US10351905B2 (en) | 2010-02-12 | 2019-07-16 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
| US9366632B2 (en) | 2010-02-12 | 2016-06-14 | Raindance Technologies, Inc. | Digital analyte analysis |
| WO2012045012A2 (en) | 2010-09-30 | 2012-04-05 | Raindance Technologies, Inc. | Sandwich assays in droplets |
| WO2012079749A1 (en) | 2010-12-16 | 2012-06-21 | Stichting Dutch Polymer Institute | Process for making cell suspension |
| EP3859011A1 (en) | 2011-02-11 | 2021-08-04 | Bio-Rad Laboratories, Inc. | Methods for forming mixed droplets |
| WO2012112804A1 (en) | 2011-02-18 | 2012-08-23 | Raindance Technoligies, Inc. | Compositions and methods for molecular labeling |
| BR112013029729A2 (pt) | 2011-05-23 | 2017-01-24 | Basf Se | controle de emulsões, incluindo emulsões múltiplas |
| EP2714970B1 (en) | 2011-06-02 | 2017-04-19 | Raindance Technologies, Inc. | Enzyme quantification |
| US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
| EP2729238A2 (en) | 2011-07-06 | 2014-05-14 | President and Fellows of Harvard College | Multiple emulsions and techniques for the formation of multiple emulsions |
| US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
| US20150177115A1 (en) | 2012-04-06 | 2015-06-25 | Slingshot Biosciences | Hydrogel particles with tunable optical properties |
| WO2013155525A1 (en) * | 2012-04-13 | 2013-10-17 | Biolumix, Inc | Ultra rapid blood culturing and susceptibility testing system |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CN114891871A (zh) | 2012-08-14 | 2022-08-12 | 10X基因组学有限公司 | 微胶囊组合物及方法 |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP2708890B1 (en) * | 2012-09-14 | 2015-11-18 | Molecular Devices (New Milton) Ltd | Method of selecting a monoclonal cell colony |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP3567116A1 (en) | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| KR20230003659A (ko) | 2013-02-08 | 2023-01-06 | 10엑스 제노믹스, 인크. | 폴리뉴클레오티드 바코드 생성 |
| EP2972230B1 (en) | 2013-03-15 | 2021-07-21 | Hycor Biomedical LLC | Device and associated method for performing luminescence and fluorescence measurements of a sample |
| JP2016527494A (ja) | 2013-07-05 | 2016-09-08 | ユニバーシティ オブ ワシントン スルー イッツ センター フォー コマーシャライゼーション | マイクロ流体アッセイのための方法、組成物およびシステム |
| US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
| US11901041B2 (en) | 2013-10-04 | 2024-02-13 | Bio-Rad Laboratories, Inc. | Digital analysis of nucleic acid modification |
| WO2015069634A1 (en) | 2013-11-08 | 2015-05-14 | President And Fellows Of Harvard College | Microparticles, methods for their preparation and use |
| US9944977B2 (en) | 2013-12-12 | 2018-04-17 | Raindance Technologies, Inc. | Distinguishing rare variations in a nucleic acid sequence from a sample |
| US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
| WO2015103367A1 (en) | 2013-12-31 | 2015-07-09 | Raindance Technologies, Inc. | System and method for detection of rna species |
| GB201402591D0 (en) * | 2014-02-14 | 2014-04-02 | Memo Therapeutics Ag | Method for recovering two or more genes, or gene products, encoding an immunoreceptor |
| US9694361B2 (en) | 2014-04-10 | 2017-07-04 | 10X Genomics, Inc. | Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same |
| EP4574974A3 (en) | 2014-06-26 | 2025-10-08 | 10x Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| KR20170026383A (ko) | 2014-06-26 | 2017-03-08 | 10엑스 제노믹스, 인크. | 핵산 서열의 분석 |
| EP4235677A3 (en) | 2014-06-26 | 2023-11-22 | 10X Genomics, Inc. | Processes and systems for nucleic acid sequence assembly |
| JP2017532042A (ja) | 2014-10-29 | 2017-11-02 | 10エックス ゲノミクス,インコーポレイテッド | 標的化核酸配列決定のための方法及び組成物 |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| AU2016207023B2 (en) | 2015-01-12 | 2019-12-05 | 10X Genomics, Inc. | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
| CA2968417A1 (en) | 2015-01-13 | 2016-07-21 | 10X Genomics, Inc. | Systems and methods for visualizing structural variation and phasing information |
| DK3256850T3 (da) | 2015-02-09 | 2025-08-18 | Slingshot Biosciences Inc | Hydrogelpartikler med justerbare optiske egenskaber og fremgangsmåder til anvendelse deraf |
| SG11201705996PA (en) | 2015-02-09 | 2017-09-28 | 10X Genomics Inc | Systems and methods for determining structural variation and phasing using variant call data |
| EP4286516A3 (en) | 2015-02-24 | 2024-03-06 | 10X Genomics, Inc. | Partition processing methods and systems |
| AU2016222719B2 (en) | 2015-02-24 | 2022-03-31 | 10X Genomics, Inc. | Methods for targeted nucleic acid sequence coverage |
| FR3033333A1 (fr) * | 2015-03-06 | 2016-09-09 | Commissariat Energie Atomique | Procede et dispositif pour detecter en temps reel un compose secrete et la cible secretrice ainsi que leurs utilisations |
| WO2016145242A1 (en) * | 2015-03-11 | 2016-09-15 | Agenus Inc. | Methods and compositions for high throughput screening of biomolecules using gel microdrops |
| US10647981B1 (en) | 2015-09-08 | 2020-05-12 | Bio-Rad Laboratories, Inc. | Nucleic acid library generation methods and compositions |
| JP6785465B2 (ja) * | 2015-10-02 | 2020-11-18 | 国立大学法人広島大学 | 微生物の分離培養方法及び凝集体含有溶液 |
| US11123297B2 (en) | 2015-10-13 | 2021-09-21 | President And Fellows Of Harvard College | Systems and methods for making and using gel microspheres |
| US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
| KR102723865B1 (ko) | 2015-12-04 | 2024-10-29 | 10엑스 제노믹스, 인크. | 핵산 분석을 위한 방법 및 조성물 |
| WO2017132627A2 (en) * | 2016-01-29 | 2017-08-03 | Achaogen, Inc. | Screening methods for identifying antibodies that bind cell surface epitopes |
| JP6735348B2 (ja) | 2016-02-11 | 2020-08-05 | 10エックス ジェノミクス, インコーポレイテッド | 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体 |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| TW201825511A (zh) | 2016-09-09 | 2018-07-16 | 美商艾斯合顧問有限公司 | 表現免疫檢查點調節子的溶瘤病毒 |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP3343223A1 (en) * | 2016-12-30 | 2018-07-04 | Blink AG | A prefabricated microparticle for performing a digital detection of an analyte |
| EP4310183B1 (en) | 2017-01-30 | 2025-07-09 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| WO2018145041A1 (en) | 2017-02-06 | 2018-08-09 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
| DK4218738T3 (da) | 2017-02-24 | 2024-11-11 | Univ California | Partikeldråbestrukturer og fremgangsmåder til fremstilling og anvendelse af disse |
| EP3615574B1 (en) | 2017-04-26 | 2026-06-24 | Eureka Therapeutics, Inc. | Constructs specifically recognizing glypican 3 and uses thereof |
| WO2018213774A1 (en) | 2017-05-19 | 2018-11-22 | 10X Genomics, Inc. | Systems and methods for analyzing datasets |
| US10844372B2 (en) | 2017-05-26 | 2020-11-24 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| CN116064732A (zh) | 2017-05-26 | 2023-05-05 | 10X基因组学有限公司 | 转座酶可接近性染色质的单细胞分析 |
| EP3625361A1 (en) | 2017-11-15 | 2020-03-25 | 10X Genomics, Inc. | Functionalized gel beads |
| WO2019099908A1 (en) | 2017-11-17 | 2019-05-23 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| EP3752832A1 (en) | 2018-02-12 | 2020-12-23 | 10X Genomics, Inc. | Methods characterizing multiple analytes from individual cells or cell populations |
| WO2019176275A1 (ja) * | 2018-03-12 | 2019-09-19 | ソニー株式会社 | 細胞評価装置及び細胞評価システム |
| EP3775271B1 (en) | 2018-04-06 | 2025-03-12 | 10X Genomics, Inc. | Systems and methods for quality control in single cell processing |
| WO2019217758A1 (en) | 2018-05-10 | 2019-11-14 | 10X Genomics, Inc. | Methods and systems for molecular library generation |
| US20200032335A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
| US11459607B1 (en) | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
| US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
| US11851683B1 (en) | 2019-02-12 | 2023-12-26 | 10X Genomics, Inc. | Methods and systems for selective analysis of cellular samples |
| CN110498858B (zh) * | 2019-07-26 | 2024-01-23 | 深圳市达科为生物工程有限公司 | 一种动态检测单细胞外泌蛋白分泌情况的方法 |
| US12235262B1 (en) | 2019-09-09 | 2025-02-25 | 10X Genomics, Inc. | Methods and systems for single cell protein analysis |
| TW202141019A (zh) | 2020-01-24 | 2021-11-01 | 美商史林夏特生化公司 | 類細胞校正粒子之組合物與方法 |
| EP4104179B1 (en) | 2020-02-13 | 2026-01-28 | 10X Genomics, Inc. | Systems and methods for joint interactive visualization of gene expression and dna chromatin accessibility |
| CA3177834A1 (en) | 2020-05-04 | 2021-11-11 | Jeffrey Kim | Compositions and methods for passive optical barcoding for multiplexed assays |
| WO2022147481A1 (en) | 2020-12-30 | 2022-07-07 | Ansun Biopharma Inc. | Combination therapy of an oncolytic virus delivering a foreign antigen and an engineered immune cell expressing a chimeric receptor targeting the foreign antigen |
| US20250264477A1 (en) | 2021-04-14 | 2025-08-21 | Partillion Bioscience Corporation | Nanoscale reaction chambers and methods of using the same |
| CN113917159A (zh) * | 2021-09-30 | 2022-01-11 | 杭州联科生物技术股份有限公司 | 一种淋巴细胞亚群的流式检测方法 |
| JP2024539956A (ja) | 2021-10-29 | 2024-10-31 | スリングショット バイオサイエンシーズ, インコーポレイテッド | フィーダー細胞としておよび合成抗原提示細胞としてのヒドロゲル粒子 |
| JP2025503454A (ja) * | 2021-12-17 | 2025-02-04 | ウィリアム マーシュ ライス ユニバーシティ | Il-12を発現するカプセル化細胞及びその使用 |
| AU2023265684A1 (en) | 2022-05-05 | 2024-11-21 | Slingshot Biosciences, Inc. | Engineered particles as red blood cell mimics and compositions containing same for hematology |
| CA3257621A1 (en) | 2022-06-02 | 2023-12-07 | Slingshot Biosciences, Inc. | APOPTOTIC CELL MIMETIC |
| WO2024092161A2 (en) | 2022-10-26 | 2024-05-02 | Slingshot Biosciences, Inc. | Size-tunable synthetic particles with tunable optical properties and methods for using the same for immune cell activation |
| US12461007B2 (en) | 2023-08-29 | 2025-11-04 | Slingshot Biosciences, Inc. | CD34 stem cell mimics |
| WO2026115815A1 (ja) * | 2024-11-29 | 2026-06-04 | ソニーグループ株式会社 | 分泌物評価方法、及び分泌物評価システム |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4801529A (en) * | 1985-06-18 | 1989-01-31 | Brandeis University | Methods for isolating mutant microoganisms using microcapsules coated with indicator material |
| US4959301A (en) | 1988-04-22 | 1990-09-25 | Massachusetts Institute Of Technology | Process for rapidly enumerating viable entities |
| US5225332A (en) * | 1988-04-22 | 1993-07-06 | Massachusetts Institute Of Technology | Process for manipulation of non-aqueous surrounded microdroplets |
| US5888728A (en) | 1988-10-17 | 1999-03-30 | Molecular Devices Corporation | Hapten derivatized capture membrane and diagnostic assays using such membrane |
| US5965379A (en) * | 1991-07-19 | 1999-10-12 | Cytimmune Sciences Inc. | Method for measuring endogenous cytokines |
| US6309883B1 (en) * | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US20030215798A1 (en) * | 1997-06-16 | 2003-11-20 | Diversa Corporation | High throughput fluorescence-based screening for novel enzymes |
| US6068979A (en) * | 1998-04-22 | 2000-05-30 | Lumigen, Inc. | Simplified sequential chemiluminescent detection |
| GB9928193D0 (en) | 1999-11-29 | 2000-01-26 | Univ Bristol | Cell treatment method |
| JP2003523180A (ja) | 1999-12-03 | 2003-08-05 | ザ・トラステイーズ・オブ・ザ・ユニバーシテイ・オブ・ペンシルベニア | 多重のパッケージングシグナルを用いる組み換えアデノウイルスのパッケージングおよび収量を増加するための組成物および方法 |
-
2002
- 2002-05-28 WO PCT/US2002/016783 patent/WO2002097122A1/en not_active Ceased
- 2002-05-28 DE DE60229246T patent/DE60229246D1/de not_active Expired - Lifetime
- 2002-05-28 AU AU2002314820A patent/AU2002314820B2/en not_active Ceased
- 2002-05-28 AT AT02741743T patent/ATE410680T1/de not_active IP Right Cessation
- 2002-05-28 US US10/157,351 patent/US6806058B2/en not_active Expired - Fee Related
- 2002-05-28 EP EP02741743A patent/EP1399580B1/en not_active Expired - Lifetime
- 2002-05-28 CA CA002447691A patent/CA2447691A1/en not_active Abandoned
- 2002-05-28 JP JP2003500287A patent/JP4194934B2/ja not_active Expired - Fee Related
-
2004
- 2004-08-13 US US10/917,673 patent/US7556928B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2447691A1 (en) | 2002-12-05 |
| US6806058B2 (en) | 2004-10-19 |
| AU2002314820B2 (en) | 2008-01-24 |
| EP1399580A4 (en) | 2005-06-08 |
| US7556928B2 (en) | 2009-07-07 |
| WO2002097122A1 (en) | 2002-12-05 |
| EP1399580B1 (en) | 2008-10-08 |
| US20030143642A1 (en) | 2003-07-31 |
| EP1399580A1 (en) | 2004-03-24 |
| DE60229246D1 (enExample) | 2008-11-20 |
| ATE410680T1 (de) | 2008-10-15 |
| JP2004528574A (ja) | 2004-09-16 |
| US20050019839A1 (en) | 2005-01-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6806058B2 (en) | Secretions of proteins by encapsulated cells | |
| AU2002314820A1 (en) | Secretion of Molecules by Encapsulated Cells | |
| JP4450998B2 (ja) | フローサイトメトリの全血樹状細胞免疫機能アッセイ | |
| Powell et al. | Gel microdroplets and flow cytometry: rapid determination of antibody secretion by individual cells within a cell population | |
| AU2014232225B2 (en) | Protocol for identifying and isolating antigen-specific B cells and producing antibodies to desired antigens | |
| Gray et al. | Secretion capture and report web: use of affinity derivatized agarose microdroplets for the selection of hybridoma cells | |
| Caraher et al. | Flow cytometric analysis of intracellular IFN-γ, IL-4 and IL-10 in CD3+ 4+ T-cells from rat spleen | |
| WO2009140876A1 (zh) | 从生物体液样本中富集与检测稀有细胞的整合方法 | |
| EP2032695A2 (en) | Cd127 expression inversely correlates with foxp3 and suppressive function of cd4+ tregs | |
| WO2005012505A2 (en) | Nanoparticle and microparticle based detection of cellular products | |
| Tai et al. | Isolation and characterization of human multiple myeloma cell enriched populations | |
| US20190352607A1 (en) | Methods for expanding and differentiating b cells for producing antibody | |
| JP2025114771A (ja) | マルチパラメータサイトメトリーに基づく白血球サブセット化の為の手段及び方法 | |
| WO2009103753A1 (en) | Methods for identifying and/or sorting cells by secreted molecule and kits for performing such methods | |
| Zola | Detection of cytokine receptors by flow cytometry | |
| Collins | Cytokine and cytokine receptor expression as a biological indicator of immune activation: important considerations in the development of in vitro model systems | |
| CN110178034A (zh) | 用于从生物样品中测定免疫球蛋白γ(IgG)抗体同种型浓度的方法和试剂 | |
| US9063145B2 (en) | Multivalent opsonophagocytic assay using bead array and imaging | |
| AU2003243273A1 (en) | Detection of secreted polypeptides | |
| US8771971B2 (en) | Methods and kits for measurement of lymphocyte function | |
| Chesneau et al. | New method for the expansion of highly purified human regulatory granzyme B-expressing B cells | |
| Loza et al. | Multiple color immunofluorescence for cytokine detection at the single-cell level | |
| CN112921004B (zh) | 用于cd3受体激动剂检测的细胞株及其构建方法及cd3受体激动剂检测方法 | |
| KR20230118134A (ko) | CD6 high 세포 선택적 표적화 및 T eff 세포의 활성감소 방법 | |
| HK40119794A (en) | Methods for donor cell analysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20050105 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20050105 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20071205 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20071214 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20080311 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20080318 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20080410 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20080417 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20080512 |
|
| RD04 | Notification of resignation of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7424 Effective date: 20080610 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20080903 |
|
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20080924 |
|
| R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20111003 Year of fee payment: 3 |
|
| LAPS | Cancellation because of no payment of annual fees |