JP4047170B2 - 酵母発現ベクターおよび酵母細胞における発現による組換えタンパク質の製造方法 - Google Patents
酵母発現ベクターおよび酵母細胞における発現による組換えタンパク質の製造方法 Download PDFInfo
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- JP4047170B2 JP4047170B2 JP2002551170A JP2002551170A JP4047170B2 JP 4047170 B2 JP4047170 B2 JP 4047170B2 JP 2002551170 A JP2002551170 A JP 2002551170A JP 2002551170 A JP2002551170 A JP 2002551170A JP 4047170 B2 JP4047170 B2 JP 4047170B2
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin
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- Chemical & Material Sciences (AREA)
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- Urology & Nephrology (AREA)
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- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0031196A GB0031196D0 (en) | 2000-12-21 | 2000-12-21 | Recombinant human plasminogen and plasmin: methods for high yield production stabilization and use for treatment |
| GB0116690A GB0116690D0 (en) | 2001-07-09 | 2001-07-09 | Human plasminogen and plasmin |
| GB0116702A GB0116702D0 (en) | 2001-07-09 | 2001-07-09 | Human plasminogen and plasmin |
| PCT/BE2001/000217 WO2002050290A1 (en) | 2000-12-21 | 2001-12-20 | A yeast expression vector and a method of making a recombinant protein by expression in a yeast cell |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007067213A Division JP4250660B2 (ja) | 2000-12-21 | 2007-03-15 | 活性化されたミクロプラスミンの産生方法 |
Publications (3)
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| JP2004526427A JP2004526427A (ja) | 2004-09-02 |
| JP2004526427A5 JP2004526427A5 (https=) | 2005-05-19 |
| JP4047170B2 true JP4047170B2 (ja) | 2008-02-13 |
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| JP2002551170A Expired - Lifetime JP4047170B2 (ja) | 2000-12-21 | 2001-12-20 | 酵母発現ベクターおよび酵母細胞における発現による組換えタンパク質の製造方法 |
| JP2007067213A Expired - Lifetime JP4250660B2 (ja) | 2000-12-21 | 2007-03-15 | 活性化されたミクロプラスミンの産生方法 |
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| Application Number | Title | Priority Date | Filing Date |
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| JP2007067213A Expired - Lifetime JP4250660B2 (ja) | 2000-12-21 | 2007-03-15 | 活性化されたミクロプラスミンの産生方法 |
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| Country | Link |
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| US (1) | US7445775B2 (https=) |
| EP (1) | EP1343903B1 (https=) |
| JP (2) | JP4047170B2 (https=) |
| AT (1) | ATE378413T1 (https=) |
| AU (1) | AU2002218890A1 (https=) |
| CY (1) | CY1107866T1 (https=) |
| DE (1) | DE60131450T2 (https=) |
| DK (1) | DK1343903T3 (https=) |
| ES (1) | ES2296705T3 (https=) |
| PT (1) | PT1343903E (https=) |
| WO (1) | WO2002050290A1 (https=) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US6998121B2 (en) * | 2003-01-23 | 2006-02-14 | Milkhaus Laboratory, Inc. | Method of treatment of connective tissue disorders by administration of streptolysin O |
| DE10390418D2 (de) * | 2002-02-06 | 2005-01-13 | N Zyme Biotec Gmbh | Verfahren zur Herstellung von rekombinanten Proteinen in Mikroorganismen |
| AU2003210137A1 (en) * | 2002-02-06 | 2003-09-02 | N-Zyme Biotec Gmbh | Method for producing recombinant proteins in micro-organisms |
| GB0228409D0 (en) | 2002-12-06 | 2003-01-08 | Thromb X Nv | Pharmacological vitreolysis |
| KR100595864B1 (ko) * | 2004-01-27 | 2006-06-30 | 재단법인 목암생명공학연구소 | 형질전환 사카로마이세스 세레비지애 균주 및 이를 이용한ｌｋ8 단백질의 생산방법 |
| CN1938427A (zh) | 2004-02-11 | 2007-03-28 | 比奥莱克斯公司 | 纤溶酶原和微纤溶酶原在浮萍中的表达 |
| CN102660564B (zh) * | 2004-04-22 | 2013-05-08 | 泰勒克里斯生物治疗学公司 | 重组修饰的纤溶酶 |
| CN100368524C (zh) * | 2005-05-17 | 2008-02-13 | 上海大学 | 巴士德毕赤酵母gs115/pfk-k5的高密度发酵方法 |
| US20070134231A1 (en) * | 2005-12-13 | 2007-06-14 | Jani Dharmendra M | Method for prolonging activity of autodegradable enzymes and compositions thereof |
| US20070134230A1 (en) * | 2005-12-13 | 2007-06-14 | Jani Dharmendra M | Method for prolonging activity of autodegradable enzymes |
| US20070196350A1 (en) * | 2006-02-22 | 2007-08-23 | Bartels Stephen P | Compositions and Methods for Effecting Controlled Posterior Vitreous Detachment |
| US20070212358A1 (en) * | 2006-03-10 | 2007-09-13 | Bartels Stephen P | Compositions and Methods for Effecting Controlled Posterior Vitreous Detachment |
| PL2220221T3 (pl) * | 2007-11-29 | 2015-07-31 | Grifols Therapeutics Inc | Rekombinacyjnie zmodyfikowana plazmina |
| WO2009104199A1 (en) * | 2008-02-19 | 2009-08-27 | Biocon Limited | A method of obtaining purified heterologous insulins expressed in yeast |
| MX2010013282A (es) | 2008-06-04 | 2010-12-21 | Talecris Biotherapeutics Inc | Composicion, metodo y kit para la preparacion de plasmina. |
| HUE025670T2 (en) | 2009-03-03 | 2016-04-28 | Grifols Therapeutics Inc | A method for producing plasminogen |
| WO2011004011A1 (en) | 2009-07-10 | 2011-01-13 | Thrombogenics Nv | Variants of plasminogen and plasmin |
| ES2534911T3 (es) | 2009-08-28 | 2015-04-30 | Thrombogenics N.V. | Uso de plasmina para el tratamiento de fallo de filtración después de trabeculectomía |
| JP6085568B2 (ja) | 2011-01-05 | 2017-02-22 | スロンボジェニックス・ナムローゼ・フェンノートシャップＴｈｒｏｍｂｏＧｅｎｉｃｓ ＮＶ | プラスミノーゲンおよびプラスミンの変異体 |
| US9644196B2 (en) | 2011-08-12 | 2017-05-09 | Thrombogenics Nv | Plasminogen and plasmin variants |
| CN110066783B (zh) * | 2019-05-16 | 2021-07-13 | 重庆派金生物科技有限公司 | 一种无自切形式的微纤溶酶制备方法 |
| CN113355309B (zh) * | 2021-08-10 | 2021-11-05 | 迈威(上海)生物科技股份有限公司 | 重组截短型人纤维蛋白溶酶制备工艺 |
Family Cites Families (5)
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| US4774087A (en) * | 1987-08-14 | 1988-09-27 | Northwestern University | Micro-size fibrinolytic plasmin |
| WO1990013640A1 (en) | 1989-05-01 | 1990-11-15 | The University Of Notre Dame Du Lac | Methods and materials for expression of human plasminogen in a eukaryotic cell system |
| GB9222758D0 (en) * | 1992-10-29 | 1992-12-09 | British Bio Technology | Proteins and nucleic acids |
| US5288489A (en) | 1991-08-28 | 1994-02-22 | Orion Therapeutic Systems, Inc. | Fibrinolysis and fibrinogenolysis treatment |
| ATE349226T1 (de) * | 1998-09-29 | 2007-01-15 | Leuven Res & Dev Vzw | Verwendung von verbindungen die alpha2- antiplasmin in vivo reduzieren zur herstellung einer zusammensetzung zur behandlung ischämischer schlaganfälle |
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2001
- 2001-12-20 JP JP2002551170A patent/JP4047170B2/ja not_active Expired - Lifetime
- 2001-12-20 DK DK01271444T patent/DK1343903T3/da active
- 2001-12-20 ES ES01271444T patent/ES2296705T3/es not_active Expired - Lifetime
- 2001-12-20 US US10/450,976 patent/US7445775B2/en not_active Expired - Lifetime
- 2001-12-20 AU AU2002218890A patent/AU2002218890A1/en not_active Abandoned
- 2001-12-20 PT PT01271444T patent/PT1343903E/pt unknown
- 2001-12-20 AT AT01271444T patent/ATE378413T1/de active
- 2001-12-20 WO PCT/BE2001/000217 patent/WO2002050290A1/en not_active Ceased
- 2001-12-20 DE DE60131450T patent/DE60131450T2/de not_active Expired - Lifetime
- 2001-12-20 EP EP01271444A patent/EP1343903B1/en not_active Expired - Lifetime
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2007
- 2007-03-15 JP JP2007067213A patent/JP4250660B2/ja not_active Expired - Lifetime
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2008
- 2008-01-25 CY CY20081100089T patent/CY1107866T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU2002218890A1 (en) | 2002-07-01 |
| DE60131450D1 (de) | 2007-12-27 |
| ES2296705T3 (es) | 2008-05-01 |
| JP4250660B2 (ja) | 2009-04-08 |
| US7445775B2 (en) | 2008-11-04 |
| EP1343903A1 (en) | 2003-09-17 |
| EP1343903B1 (en) | 2007-11-14 |
| PT1343903E (pt) | 2008-02-18 |
| CY1107866T1 (el) | 2013-06-19 |
| JP2004526427A (ja) | 2004-09-02 |
| DK1343903T3 (da) | 2008-03-10 |
| US20040071676A1 (en) | 2004-04-15 |
| DE60131450T2 (de) | 2008-10-16 |
| WO2002050290A1 (en) | 2002-06-27 |
| ATE378413T1 (de) | 2007-11-15 |
| JP2007195558A (ja) | 2007-08-09 |
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Free format text: JAPANESE INTERMEDIATE CODE: R250 |
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| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
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Free format text: JAPANESE INTERMEDIATE CODE: R250 |
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