ATE378413T1 - Hefeexpressionsvektor und verfahren zur herstellung eines rekombinanten proteins durch expression in einer hefezelle - Google Patents

Hefeexpressionsvektor und verfahren zur herstellung eines rekombinanten proteins durch expression in einer hefezelle

Info

Publication number
ATE378413T1
ATE378413T1 AT01271444T AT01271444T ATE378413T1 AT E378413 T1 ATE378413 T1 AT E378413T1 AT 01271444 T AT01271444 T AT 01271444T AT 01271444 T AT01271444 T AT 01271444T AT E378413 T1 ATE378413 T1 AT E378413T1
Authority
AT
Austria
Prior art keywords
expression
yeast
producing
recombinant protein
expression vector
Prior art date
Application number
AT01271444T
Other languages
German (de)
English (en)
Inventor
Desire Collen
Nubuo Nagai
Yves Laroche
Original Assignee
Thromb X N V
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0031196A external-priority patent/GB0031196D0/en
Priority claimed from GB0116690A external-priority patent/GB0116690D0/en
Priority claimed from GB0116702A external-priority patent/GB0116702D0/en
Application filed by Thromb X N V filed Critical Thromb X N V
Application granted granted Critical
Publication of ATE378413T1 publication Critical patent/ATE378413T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Vascular Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
AT01271444T 2000-12-21 2001-12-20 Hefeexpressionsvektor und verfahren zur herstellung eines rekombinanten proteins durch expression in einer hefezelle ATE378413T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0031196A GB0031196D0 (en) 2000-12-21 2000-12-21 Recombinant human plasminogen and plasmin: methods for high yield production stabilization and use for treatment
GB0116690A GB0116690D0 (en) 2001-07-09 2001-07-09 Human plasminogen and plasmin
GB0116702A GB0116702D0 (en) 2001-07-09 2001-07-09 Human plasminogen and plasmin

Publications (1)

Publication Number Publication Date
ATE378413T1 true ATE378413T1 (de) 2007-11-15

Family

ID=27256020

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01271444T ATE378413T1 (de) 2000-12-21 2001-12-20 Hefeexpressionsvektor und verfahren zur herstellung eines rekombinanten proteins durch expression in einer hefezelle

Country Status (11)

Country Link
US (1) US7445775B2 (https=)
EP (1) EP1343903B1 (https=)
JP (2) JP4047170B2 (https=)
AT (1) ATE378413T1 (https=)
AU (1) AU2002218890A1 (https=)
CY (1) CY1107866T1 (https=)
DE (1) DE60131450T2 (https=)
DK (1) DK1343903T3 (https=)
ES (1) ES2296705T3 (https=)
PT (1) PT1343903E (https=)
WO (1) WO2002050290A1 (https=)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6998121B2 (en) * 2003-01-23 2006-02-14 Milkhaus Laboratory, Inc. Method of treatment of connective tissue disorders by administration of streptolysin O
DE10390418D2 (de) * 2002-02-06 2005-01-13 N Zyme Biotec Gmbh Verfahren zur Herstellung von rekombinanten Proteinen in Mikroorganismen
AU2003210137A1 (en) * 2002-02-06 2003-09-02 N-Zyme Biotec Gmbh Method for producing recombinant proteins in micro-organisms
GB0228409D0 (en) 2002-12-06 2003-01-08 Thromb X Nv Pharmacological vitreolysis
KR100595864B1 (ko) * 2004-01-27 2006-06-30 재단법인 목암생명공학연구소 형질전환 사카로마이세스 세레비지애 균주 및 이를 이용한ｌｋ8 단백질의 생산방법
CN1938427A (zh) 2004-02-11 2007-03-28 比奥莱克斯公司 纤溶酶原和微纤溶酶原在浮萍中的表达
CN102660564B (zh) * 2004-04-22 2013-05-08 泰勒克里斯生物治疗学公司 重组修饰的纤溶酶
CN100368524C (zh) * 2005-05-17 2008-02-13 上海大学 巴士德毕赤酵母gs115/pfk-k5的高密度发酵方法
US20070134231A1 (en) * 2005-12-13 2007-06-14 Jani Dharmendra M Method for prolonging activity of autodegradable enzymes and compositions thereof
US20070134230A1 (en) * 2005-12-13 2007-06-14 Jani Dharmendra M Method for prolonging activity of autodegradable enzymes
US20070196350A1 (en) * 2006-02-22 2007-08-23 Bartels Stephen P Compositions and Methods for Effecting Controlled Posterior Vitreous Detachment
US20070212358A1 (en) * 2006-03-10 2007-09-13 Bartels Stephen P Compositions and Methods for Effecting Controlled Posterior Vitreous Detachment
PL2220221T3 (pl) * 2007-11-29 2015-07-31 Grifols Therapeutics Inc Rekombinacyjnie zmodyfikowana plazmina
WO2009104199A1 (en) * 2008-02-19 2009-08-27 Biocon Limited A method of obtaining purified heterologous insulins expressed in yeast
MX2010013282A (es) 2008-06-04 2010-12-21 Talecris Biotherapeutics Inc Composicion, metodo y kit para la preparacion de plasmina.
HUE025670T2 (en) 2009-03-03 2016-04-28 Grifols Therapeutics Inc A method for producing plasminogen
WO2011004011A1 (en) 2009-07-10 2011-01-13 Thrombogenics Nv Variants of plasminogen and plasmin
ES2534911T3 (es) 2009-08-28 2015-04-30 Thrombogenics N.V. Uso de plasmina para el tratamiento de fallo de filtración después de trabeculectomía
JP6085568B2 (ja) 2011-01-05 2017-02-22 スロンボジェニックス・ナムローゼ・フェンノートシャップＴｈｒｏｍｂｏＧｅｎｉｃｓ ＮＶ プラスミノーゲンおよびプラスミンの変異体
US9644196B2 (en) 2011-08-12 2017-05-09 Thrombogenics Nv Plasminogen and plasmin variants
CN110066783B (zh) * 2019-05-16 2021-07-13 重庆派金生物科技有限公司 一种无自切形式的微纤溶酶制备方法
CN113355309B (zh) * 2021-08-10 2021-11-05 迈威(上海)生物科技股份有限公司 重组截短型人纤维蛋白溶酶制备工艺

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4774087A (en) * 1987-08-14 1988-09-27 Northwestern University Micro-size fibrinolytic plasmin
WO1990013640A1 (en) 1989-05-01 1990-11-15 The University Of Notre Dame Du Lac Methods and materials for expression of human plasminogen in a eukaryotic cell system
GB9222758D0 (en) * 1992-10-29 1992-12-09 British Bio Technology Proteins and nucleic acids
US5288489A (en) 1991-08-28 1994-02-22 Orion Therapeutic Systems, Inc. Fibrinolysis and fibrinogenolysis treatment
ATE349226T1 (de) * 1998-09-29 2007-01-15 Leuven Res & Dev Vzw Verwendung von verbindungen die alpha2- antiplasmin in vivo reduzieren zur herstellung einer zusammensetzung zur behandlung ischämischer schlaganfälle

Also Published As

Publication number Publication date
AU2002218890A1 (en) 2002-07-01
JP4047170B2 (ja) 2008-02-13
DE60131450D1 (de) 2007-12-27
ES2296705T3 (es) 2008-05-01
JP4250660B2 (ja) 2009-04-08
US7445775B2 (en) 2008-11-04
EP1343903A1 (en) 2003-09-17
EP1343903B1 (en) 2007-11-14
PT1343903E (pt) 2008-02-18
CY1107866T1 (el) 2013-06-19
JP2004526427A (ja) 2004-09-02
DK1343903T3 (da) 2008-03-10
US20040071676A1 (en) 2004-04-15
DE60131450T2 (de) 2008-10-16
WO2002050290A1 (en) 2002-06-27
JP2007195558A (ja) 2007-08-09

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