JP3140488B2 - 融合タンパク質の試験管内プロセシング - Google Patents

融合タンパク質の試験管内プロセシング

Info

Publication number
JP3140488B2
JP3140488B2 JP03176552A JP17655291A JP3140488B2 JP 3140488 B2 JP3140488 B2 JP 3140488B2 JP 03176552 A JP03176552 A JP 03176552A JP 17655291 A JP17655291 A JP 17655291A JP 3140488 B2 JP3140488 B2 JP 3140488B2
Authority
JP
Japan
Prior art keywords
protein
amino acid
lys
arg
ser
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP03176552A
Other languages
English (en)
Japanese (ja)
Other versions
JPH04229188A (ja
Inventor
ハイム ユッタ
シーボツ ペーター
タカバヤシ ケンジ
Original Assignee
ノバルティス アクチエンゲゼルシャフト
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ノバルティス アクチエンゲゼルシャフト filed Critical ノバルティス アクチエンゲゼルシャフト
Publication of JPH04229188A publication Critical patent/JPH04229188A/ja
Application granted granted Critical
Publication of JP3140488B2 publication Critical patent/JP3140488B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Mycology (AREA)
  • Endocrinology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
JP03176552A 1990-07-18 1991-07-17 融合タンパク質の試験管内プロセシング Expired - Fee Related JP3140488B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB909015825A GB9015825D0 (en) 1990-07-18 1990-07-18 In vitro processing of fusion proteins
GB90158254 1990-07-18

Publications (2)

Publication Number Publication Date
JPH04229188A JPH04229188A (ja) 1992-08-18
JP3140488B2 true JP3140488B2 (ja) 2001-03-05

Family

ID=10679295

Family Applications (1)

Application Number Title Priority Date Filing Date
JP03176552A Expired - Fee Related JP3140488B2 (ja) 1990-07-18 1991-07-17 融合タンパク質の試験管内プロセシング

Country Status (19)

Country Link
EP (1) EP0467839B1 (enExample)
JP (1) JP3140488B2 (enExample)
AT (1) ATE141645T1 (enExample)
AU (1) AU651830B2 (enExample)
CA (1) CA2047119C (enExample)
DE (1) DE69121489T2 (enExample)
DK (1) DK0467839T3 (enExample)
ES (1) ES2091897T3 (enExample)
FI (1) FI106720B (enExample)
GB (1) GB9015825D0 (enExample)
GR (1) GR3020840T3 (enExample)
HU (1) HU216104B (enExample)
IE (1) IE73209B1 (enExample)
NO (1) NO303132B1 (enExample)
NZ (1) NZ238989A (enExample)
PT (1) PT98332B (enExample)
SG (1) SG49863A1 (enExample)
TW (1) TW209247B (enExample)
ZA (1) ZA915579B (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5525491A (en) * 1991-02-27 1996-06-11 Creative Biomolecules, Inc. Serine-rich peptide linkers
CA2213512A1 (en) * 1995-12-22 1997-07-03 Toray Industries, Inc. Method for producing bioactive fusion protein
US6800726B1 (en) 1996-11-01 2004-10-05 Pioneer Hi-Bred International, Inc. Proteins with increased levels of essential amino acids
US6265204B1 (en) 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
GB9716790D0 (en) 1997-08-07 1997-10-15 Creative Peptides Sweden Ab Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof
WO2014138371A1 (en) 2013-03-06 2014-09-12 Glaxosmithkline Llc Host cells and methods of use
CN118515782B (zh) * 2024-06-03 2025-03-18 浙江毓昌生物技术有限公司 一种融合蛋白及基于该融合蛋白制备重组人生长激素的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MC1834A1 (fr) * 1986-07-10 1988-06-03 Transgene Sa Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ＦＥＢＳ Ｌｅｔｔｅｒｓ，Ｖｏｌ．２１８［１］（１９８７）ｐ．３１−３４

Also Published As

Publication number Publication date
JPH04229188A (ja) 1992-08-18
DE69121489T2 (de) 1997-02-27
IE912501A1 (en) 1992-01-29
PT98332B (pt) 1999-01-29
GR3020840T3 (en) 1996-11-30
AU651830B2 (en) 1994-08-04
DE69121489D1 (de) 1996-09-26
HU912398D0 (en) 1991-12-30
ZA915579B (en) 1992-03-25
CA2047119C (en) 2002-10-08
TW209247B (enExample) 1993-07-11
NO912803D0 (no) 1991-07-17
FI913405A7 (fi) 1992-01-19
NZ238989A (en) 1992-09-25
AU8046391A (en) 1992-01-23
NO912803L (no) 1992-01-20
EP0467839A1 (en) 1992-01-22
CA2047119A1 (en) 1992-01-19
SG49863A1 (en) 2002-03-19
ATE141645T1 (de) 1996-09-15
FI106720B (fi) 2001-03-30
EP0467839B1 (en) 1996-08-21
ES2091897T3 (es) 1996-11-16
FI913405A0 (fi) 1991-07-15
HU216104B (hu) 1999-04-28
NO303132B1 (no) 1998-06-02
HUT58369A (en) 1992-02-28
GB9015825D0 (en) 1990-09-05
DK0467839T3 (da) 1996-09-16
IE73209B1 (en) 1997-05-07
PT98332A (pt) 1992-05-29

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