ES2091897T3 - Procesamiento in vitro de proteinas de fusion. - Google Patents
Procesamiento in vitro de proteinas de fusion.Info
- Publication number
- ES2091897T3 ES2091897T3 ES91810543T ES91810543T ES2091897T3 ES 2091897 T3 ES2091897 T3 ES 2091897T3 ES 91810543 T ES91810543 T ES 91810543T ES 91810543 T ES91810543 T ES 91810543T ES 2091897 T3 ES2091897 T3 ES 2091897T3
- Authority
- ES
- Spain
- Prior art keywords
- protein
- biologically active
- amino acid
- terminal amino
- active protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/585—Calcitonins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors (Somatomedins), e.g. IGF-1, IGF-2
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Abstract
SE PROPORCIONA UN NUEVO METODO PARA LA PRODUCCION DE UNA PROTEINA BIOLOGICAMENTE ACTIVA QUE COMPRENDE EL TRATAMIENTO DE UNA PROTEINA DE FUSION QUE CONSISTE EN 1) UNO O MULTIPLES SEGMENTOS SUCESIVOS DE PROTEINA, CADA UNO COMPUESTO DE LA ANTEDICHA PROTEINA BIOLOGICAMENTE ACTIVA, EL AMINOACIDO C-TERMINAL EL CUAL ESTA UNIDO A UNA SECUENCIA L POLIPEPTIDA DE UNION, Y 2) UN POLIPEPTIDO UNIDO AL AMINOACIDO C-TERMINAL DEL ANTEDICHO SEGMENTO(S) DE PROTEINA SUCESIVO; O CONSISTENTE EN 1) UN POLIPEPTIDO UNIDO A UN AMINOACIDO N-TERMINAL O, 2) MULTIPLES SEGMENTOS DE PROTEINAS SUCESIVOS CADA UNO COMPUESTO DE UNA SECUENCIA L DE POLIPEPTIDO DE UNION Y LA MENCIONADA PROTEINA BIOLOGICAMENTE ACTIVA, CON ENDOPROTEASA DE LEVADURA SOLUBLE YSCF Y CON CARBOXIPEPTIDASA DE LEVADURA SOLUBLE YSC(ALFA), Y AISLANDO LA MENCIONADA PROTEINA BIOLOGICAMENTE ACTIVA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB909015825A GB9015825D0 (en) | 1990-07-18 | 1990-07-18 | In vitro processing of fusion proteins |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2091897T3 true ES2091897T3 (es) | 1996-11-16 |
Family
ID=10679295
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES91810543T Expired - Lifetime ES2091897T3 (es) | 1990-07-18 | 1991-07-09 | Procesamiento in vitro de proteinas de fusion. |
Country Status (19)
Country | Link |
---|---|
EP (1) | EP0467839B1 (es) |
JP (1) | JP3140488B2 (es) |
AT (1) | ATE141645T1 (es) |
AU (1) | AU651830B2 (es) |
CA (1) | CA2047119C (es) |
DE (1) | DE69121489T2 (es) |
DK (1) | DK0467839T3 (es) |
ES (1) | ES2091897T3 (es) |
FI (1) | FI106720B (es) |
GB (1) | GB9015825D0 (es) |
GR (1) | GR3020840T3 (es) |
HU (1) | HU216104B (es) |
IE (1) | IE73209B1 (es) |
NO (1) | NO303132B1 (es) |
NZ (1) | NZ238989A (es) |
PT (1) | PT98332B (es) |
SG (1) | SG49863A1 (es) |
TW (1) | TW209247B (es) |
ZA (1) | ZA915579B (es) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5525491A (en) * | 1991-02-27 | 1996-06-11 | Creative Biomolecules, Inc. | Serine-rich peptide linkers |
WO1997023639A1 (fr) * | 1995-12-22 | 1997-07-03 | Toray Industries, Inc. | Procede de production de proteines fusionnees biologiquement actives |
US6800726B1 (en) | 1996-11-01 | 2004-10-05 | Pioneer Hi-Bred International, Inc. | Proteins with increased levels of essential amino acids |
US6265204B1 (en) | 1997-01-17 | 2001-07-24 | Genencor International, Inc. | DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi |
GB9716790D0 (en) | 1997-08-07 | 1997-10-15 | Creative Peptides Sweden Ab | Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof |
SG11201504771RA (en) | 2013-03-06 | 2015-09-29 | Glaxosmithkline Llc | Host cells and methods of use |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MC1834A1 (fr) * | 1986-07-10 | 1988-06-03 | Transgene Sa | Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation |
US5010182A (en) * | 1987-07-28 | 1991-04-23 | Chiron Corporation | DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides |
DK105489D0 (da) * | 1989-03-03 | 1989-03-03 | Novo Nordisk As | Polypeptid |
-
1990
- 1990-07-18 GB GB909015825A patent/GB9015825D0/en active Pending
-
1991
- 1991-07-09 DE DE69121489T patent/DE69121489T2/de not_active Expired - Fee Related
- 1991-07-09 DK DK91810543.8T patent/DK0467839T3/da active
- 1991-07-09 AT AT91810543T patent/ATE141645T1/de not_active IP Right Cessation
- 1991-07-09 SG SG9607721A patent/SG49863A1/en unknown
- 1991-07-09 EP EP91810543A patent/EP0467839B1/en not_active Expired - Lifetime
- 1991-07-09 ES ES91810543T patent/ES2091897T3/es not_active Expired - Lifetime
- 1991-07-15 FI FI913405A patent/FI106720B/fi active
- 1991-07-16 PT PT98332A patent/PT98332B/pt not_active IP Right Cessation
- 1991-07-16 NZ NZ238989A patent/NZ238989A/en not_active IP Right Cessation
- 1991-07-16 AU AU80463/91A patent/AU651830B2/en not_active Ceased
- 1991-07-16 CA CA002047119A patent/CA2047119C/en not_active Expired - Lifetime
- 1991-07-17 TW TW080105554A patent/TW209247B/zh active
- 1991-07-17 HU HU2398/91A patent/HU216104B/hu not_active IP Right Cessation
- 1991-07-17 ZA ZA915579A patent/ZA915579B/xx unknown
- 1991-07-17 JP JP03176552A patent/JP3140488B2/ja not_active Expired - Fee Related
- 1991-07-17 NO NO912803A patent/NO303132B1/no not_active IP Right Cessation
- 1991-07-17 IE IE250191A patent/IE73209B1/en not_active IP Right Cessation
-
1996
- 1996-08-22 GR GR960402054T patent/GR3020840T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
GB9015825D0 (en) | 1990-09-05 |
HU216104B (hu) | 1999-04-28 |
DE69121489D1 (de) | 1996-09-26 |
NO912803D0 (no) | 1991-07-17 |
DK0467839T3 (da) | 1996-09-16 |
NO912803L (no) | 1992-01-20 |
AU651830B2 (en) | 1994-08-04 |
EP0467839B1 (en) | 1996-08-21 |
AU8046391A (en) | 1992-01-23 |
PT98332A (pt) | 1992-05-29 |
FI913405A (fi) | 1992-01-19 |
JPH04229188A (ja) | 1992-08-18 |
ATE141645T1 (de) | 1996-09-15 |
NZ238989A (en) | 1992-09-25 |
TW209247B (es) | 1993-07-11 |
HU912398D0 (en) | 1991-12-30 |
HUT58369A (en) | 1992-02-28 |
IE912501A1 (en) | 1992-01-29 |
GR3020840T3 (en) | 1996-11-30 |
NO303132B1 (no) | 1998-06-02 |
PT98332B (pt) | 1999-01-29 |
FI913405A0 (fi) | 1991-07-15 |
SG49863A1 (en) | 2002-03-19 |
CA2047119A1 (en) | 1992-01-19 |
DE69121489T2 (de) | 1997-02-27 |
CA2047119C (en) | 2002-10-08 |
EP0467839A1 (en) | 1992-01-22 |
FI106720B (fi) | 2001-03-30 |
JP3140488B2 (ja) | 2001-03-05 |
IE73209B1 (en) | 1997-05-07 |
ZA915579B (en) | 1992-03-25 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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