JP2934482B2 - マイコプラズマ含有細胞培養物の汚染除去方法 - Google Patents
マイコプラズマ含有細胞培養物の汚染除去方法Info
- Publication number
- JP2934482B2 JP2934482B2 JP2134256A JP13425690A JP2934482B2 JP 2934482 B2 JP2934482 B2 JP 2934482B2 JP 2134256 A JP2134256 A JP 2134256A JP 13425690 A JP13425690 A JP 13425690A JP 2934482 B2 JP2934482 B2 JP 2934482B2
- Authority
- JP
- Japan
- Prior art keywords
- mycoplasma
- cells
- cell culture
- animal
- amount
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000204031 Mycoplasma Species 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000004113 cell culture Methods 0.000 title claims abstract description 17
- 238000005202 decontamination Methods 0.000 title description 4
- 230000003588 decontaminative effect Effects 0.000 title description 4
- 239000003242 anti bacterial agent Substances 0.000 claims abstract description 11
- SYMHUEFSSMBHJA-UHFFFAOYSA-N 6-methylpurine Chemical compound CC1=NC=NC2=C1NC=N2 SYMHUEFSSMBHJA-UHFFFAOYSA-N 0.000 claims abstract description 10
- 230000003115 biocidal effect Effects 0.000 claims abstract description 5
- 210000004027 cell Anatomy 0.000 claims description 40
- 210000004102 animal cell Anatomy 0.000 claims description 7
- 229940088710 antibiotic agent Drugs 0.000 claims description 7
- 210000005260 human cell Anatomy 0.000 claims description 5
- 238000010367 cloning Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- DYKFCLLONBREIL-KVUCHLLUSA-N minocycline Chemical compound C([C@H]1C2)C3=C(N(C)C)C=CC(O)=C3C(=O)C1=C(O)[C@@]1(O)[C@@H]2[C@H](N(C)C)C(O)=C(C(N)=O)C1=O DYKFCLLONBREIL-KVUCHLLUSA-N 0.000 claims description 3
- 229960004023 minocycline Drugs 0.000 claims description 3
- UURAUHCOJAIIRQ-QGLSALSOSA-N tiamulin Chemical compound CCN(CC)CCSCC(=O)O[C@@H]1C[C@@](C)(C=C)[C@@H](O)[C@H](C)[C@@]23CC[C@@H](C)[C@]1(C)[C@@H]2C(=O)CC3 UURAUHCOJAIIRQ-QGLSALSOSA-N 0.000 claims description 2
- 229960004885 tiamulin Drugs 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 claims 4
- 241001430197 Mollicutes Species 0.000 abstract description 5
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 abstract 1
- 238000011109 contamination Methods 0.000 description 6
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 3
- 231100000433 cytotoxic Toxicity 0.000 description 3
- 230000001472 cytotoxic effect Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 241000699800 Cricetinae Species 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 241000204028 Mycoplasma arginini Species 0.000 description 2
- 241000204003 Mycoplasmatales Species 0.000 description 2
- 108010058516 adenosine phosphorylase Proteins 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- KMEBCRWKZZSRRT-UHFFFAOYSA-N 2-methyl-7h-purine Chemical compound CC1=NC=C2NC=NC2=N1 KMEBCRWKZZSRRT-UHFFFAOYSA-N 0.000 description 1
- 241000203022 Acholeplasma laidlawii Species 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 206010008805 Chromosomal abnormalities Diseases 0.000 description 1
- 208000031404 Chromosome Aberrations Diseases 0.000 description 1
- 241000699802 Cricetulus griseus Species 0.000 description 1
- 102000001690 Factor VIII Human genes 0.000 description 1
- 108010054218 Factor VIII Proteins 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 241000202938 Mycoplasma hyorhinis Species 0.000 description 1
- 241000202894 Mycoplasma orale Species 0.000 description 1
- 208000035415 Reinfection Diseases 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000013452 biotechnological production Methods 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229960000301 factor viii Drugs 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 238000011580 nude mouse model Methods 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- -1 steroid compounds Chemical class 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/06—Anti-neoplasic drugs, anti-retroviral drugs, e.g. azacytidine, cyclophosphamide
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3917163A DE3917163A1 (de) | 1989-05-26 | 1989-05-26 | Verfahren zur dekontamination mycoplasmenhaltiger zellkulturen |
| DE3917163.9 | 1989-05-26 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03130074A JPH03130074A (ja) | 1991-06-03 |
| JP2934482B2 true JP2934482B2 (ja) | 1999-08-16 |
Family
ID=6381444
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2134256A Expired - Lifetime JP2934482B2 (ja) | 1989-05-26 | 1990-05-25 | マイコプラズマ含有細胞培養物の汚染除去方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US5902740A (show.php) |
| EP (1) | EP0399545B1 (show.php) |
| JP (1) | JP2934482B2 (show.php) |
| KR (1) | KR100220988B1 (show.php) |
| AT (1) | ATE121131T1 (show.php) |
| AU (1) | AU617052B2 (show.php) |
| CA (1) | CA2017568A1 (show.php) |
| DE (2) | DE3917163A1 (show.php) |
| DK (1) | DK0399545T3 (show.php) |
| ES (1) | ES2072331T3 (show.php) |
| IE (1) | IE66910B1 (show.php) |
| PT (1) | PT94145B (show.php) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007222155A (ja) * | 2006-01-27 | 2007-09-06 | Shinshu Univ | ヒトリンパ管由来細胞株およびそれを用いた診断キット |
| DE102018003906A1 (de) * | 2018-05-07 | 2019-11-07 | Smart Material Printing | Verwendung von Polyoxometallaten gegen den Befall von Eukaryotenkulturen, Virenkulturen und Mikroorganismenpopulationen durch Mollicuten sowie mollicutenhemmende und -abtötende polyoxometallathaltige Stoffe und Verfahren |
| CN111117962A (zh) * | 2019-12-24 | 2020-05-08 | 武汉博士德生物工程有限公司 | 一种有效去除支原体污染的方法 |
| KR102368575B1 (ko) | 2021-08-17 | 2022-02-28 | 주식회사 퀀텀바이오닉스 | 항 마이코플라즈마 기능을 제공하는 배양용기 및 그 제조방법 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4387161A (en) * | 1981-07-16 | 1983-06-07 | Institute For Medical Research | Detection of mycoplasma infection in cell cultures |
| DE3617803A1 (de) * | 1985-11-07 | 1987-11-19 | Bayer Ag | Verwendung von gyrasehemmern zur dekontamination von mycoplasma-infizierten zellkulturen |
| JP2709604B2 (ja) * | 1988-06-29 | 1998-02-04 | 大日本製薬株式会社 | マイコプラズマ汚染の防止または除去 |
-
1989
- 1989-05-26 DE DE3917163A patent/DE3917163A1/de active Granted
-
1990
- 1990-05-24 AU AU55900/90A patent/AU617052B2/en not_active Ceased
- 1990-05-24 KR KR1019900007513A patent/KR100220988B1/ko not_active Expired - Fee Related
- 1990-05-24 PT PT94145A patent/PT94145B/pt not_active IP Right Cessation
- 1990-05-25 DK DK90109939.0T patent/DK0399545T3/da active
- 1990-05-25 CA CA002017568A patent/CA2017568A1/en not_active Abandoned
- 1990-05-25 AT AT90109939T patent/ATE121131T1/de not_active IP Right Cessation
- 1990-05-25 JP JP2134256A patent/JP2934482B2/ja not_active Expired - Lifetime
- 1990-05-25 ES ES90109939T patent/ES2072331T3/es not_active Expired - Lifetime
- 1990-05-25 IE IE189390A patent/IE66910B1/en not_active IP Right Cessation
- 1990-05-25 DE DE59008875T patent/DE59008875D1/de not_active Expired - Fee Related
- 1990-05-25 EP EP90109939A patent/EP0399545B1/de not_active Expired - Lifetime
-
1993
- 1993-02-03 US US08/012,985 patent/US5902740A/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| Ｊｏｕｒｎａｌ ｏｆ Ｉｍｍｕｎｃｌｏｇｉｃａｌ Ｍｅｔｈｏｄｓ， Ｖｏｌ．１０８，Ｎｏ．１−２，Ｐ．３９−４３（１９８８） |
| ＴＨＥ ＭＥＲＣＫ ＩＮＤＥＸ（ＴＥＮＴＨ ＥＤＩＴＩＯＮ），１９８３，Ｐ．１３５０，Ｍｏｎａｇｒａｐｈ ｎｕｍｂｅｒ９２６４ |
| ＴＨＥ ＭＥＲＣＫ ＩＮＤＥＸ（ＴＥＮＴＨ ＥＤＩＴＩＯＮ），１９８３，Ｐ．８８８，Ｍｏｎａｇｒａｐｈ ｎｕｍｂｅｒ６０６８ |
| バイオテクノロジー事典編集委員会編「バイオテクノロジー事典」（昭６２−５−５）第８３５−８３６頁 |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3917163A1 (de) | 1990-12-06 |
| US5902740A (en) | 1999-05-11 |
| EP0399545B1 (de) | 1995-04-12 |
| ATE121131T1 (de) | 1995-04-15 |
| CA2017568A1 (en) | 1990-11-26 |
| PT94145B (pt) | 1996-12-31 |
| IE66910B1 (en) | 1996-02-07 |
| KR100220988B1 (ko) | 1999-10-01 |
| EP0399545A3 (de) | 1991-01-02 |
| AU617052B2 (en) | 1991-11-14 |
| DK0399545T3 (da) | 1995-08-14 |
| EP0399545A2 (de) | 1990-11-28 |
| ES2072331T3 (es) | 1995-07-16 |
| PT94145A (pt) | 1991-01-08 |
| KR900018367A (ko) | 1990-12-21 |
| AU5590090A (en) | 1990-11-29 |
| IE901893L (en) | 1990-11-26 |
| JPH03130074A (ja) | 1991-06-03 |
| DE59008875D1 (de) | 1995-05-18 |
| DE3917163C2 (show.php) | 1991-04-25 |
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