JP2024538792A5 - - Google Patents
Info
- Publication number
- JP2024538792A5 JP2024538792A5 JP2024522383A JP2024522383A JP2024538792A5 JP 2024538792 A5 JP2024538792 A5 JP 2024538792A5 JP 2024522383 A JP2024522383 A JP 2024522383A JP 2024522383 A JP2024522383 A JP 2024522383A JP 2024538792 A5 JP2024538792 A5 JP 2024538792A5
- Authority
- JP
- Japan
- Prior art keywords
- medium
- serum
- cell line
- viral vector
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111190063.4 | 2021-10-12 | ||
| CN202111190063 | 2021-10-12 | ||
| PCT/CN2022/124873 WO2023061409A1 (zh) | 2021-10-12 | 2022-10-12 | 适应无血清悬浮培养的hek293细胞株及其应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2024538792A JP2024538792A (ja) | 2024-10-23 |
| JP2024538792A5 true JP2024538792A5 (https=) | 2025-12-05 |
Family
ID=85987273
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024522383A Pending JP2024538792A (ja) | 2021-10-12 | 2022-10-12 | 無血清浮遊培養に適したｈｅｋ２９３細胞株及びその応用 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20240425880A1 (https=) |
| EP (1) | EP4417689A4 (https=) |
| JP (1) | JP2024538792A (https=) |
| KR (1) | KR20240070587A (https=) |
| CN (1) | CN118660958A (https=) |
| WO (1) | WO2023061409A1 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN121586778A (zh) * | 2023-04-26 | 2026-02-27 | 赛诺菲 | 宿主细胞系以及用于鉴定和使用此类宿主细胞系的方法 |
| CN117089514A (zh) * | 2023-10-13 | 2023-11-21 | 思鹏生物科技(苏州)有限公司 | 提升hek293细胞系aav生产效率的细胞筛选驯化方法 |
| CN117402817B (zh) * | 2023-10-31 | 2024-09-27 | 山东丽山生物科技有限公司 | 一种无血清培养驯化贴壁293t细胞的方法及其应用 |
| WO2025171001A1 (en) | 2024-02-06 | 2025-08-14 | Regeneron Pharmaceuticals, Inc. | Compositions and methods for improved production of aav viral vectors |
| CN118222630B (zh) * | 2024-05-23 | 2024-08-27 | 江西赛基生物技术有限公司 | 一种稳转表达cd16跨膜蛋白细胞系的构建方法及应用 |
| CN119639654B (zh) * | 2024-12-24 | 2025-10-31 | 华元(上海)生物医药有限公司 | 一种人胚胎肾单克隆细胞株及其在生产慢病毒载体中的应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140315294A1 (en) * | 2011-11-24 | 2014-10-23 | Genethon | Scalable lentiviral vector production system compatible with industrial pharmaceutical applications |
| CN102604889B (zh) * | 2012-03-19 | 2013-05-08 | 中国农业科学院哈尔滨兽医研究所 | 适应无血清培养的hek293细胞系及其应用 |
| EP2970920B1 (en) * | 2013-03-15 | 2018-04-25 | The Children's Hospital of Philadelphia | Scalable manufacturing process to produce recombinant lentiviral vectors in serum-free suspension cell culture system |
| CN104450607B (zh) * | 2014-11-14 | 2017-07-11 | 武汉金开瑞生物工程有限公司 | 用于hek293细胞悬浮生长的全化学成分培养基及培养方法 |
| CN110713971B (zh) * | 2019-11-06 | 2021-08-27 | 深圳市菲鹏生物治疗股份有限公司 | 一种无血清悬浮培养型293t细胞、其制备方法以及病毒的包装方法 |
| EP4273236A4 (en) * | 2020-12-29 | 2025-01-08 | Jiangsu Genscript Probio Biotech Co., Ltd. | HEK293T CELL STRAIN WITH HIGH DISPERSIBILITY AND SCREENING METHODS THEREFOR |
-
2022
- 2022-10-12 JP JP2024522383A patent/JP2024538792A/ja active Pending
- 2022-10-12 CN CN202280068776.XA patent/CN118660958A/zh active Pending
- 2022-10-12 US US18/700,983 patent/US20240425880A1/en active Pending
- 2022-10-12 WO PCT/CN2022/124873 patent/WO2023061409A1/zh not_active Ceased
- 2022-10-12 EP EP22880345.8A patent/EP4417689A4/en active Pending
- 2022-10-12 KR KR1020247012818A patent/KR20240070587A/ko active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2024538792A5 (https=) | ||
| CN110713971B (zh) | 一种无血清悬浮培养型293t细胞、其制备方法以及病毒的包装方法 | |
| Kami et al. | Large-scale cell production of stem cells for clinical application using the automated cell processing machine | |
| JP2024504914A5 (https=) | ||
| CA2954245A1 (en) | Method for evaluating quality of human mesenchymal stem cell, and monoclonal antibody for use in said method | |
| JP6463029B2 (ja) | ヒト間葉系幹細胞を特異的に認識するモノクローナル抗体並びにこれを用いたヒト間葉系幹細胞の分離及び／または品質評価を行う方法 | |
| CN113604425A (zh) | 一种适应无血清培养基环境的wayne293 lvpro细胞及其应用 | |
| CN117050928B (zh) | 无弹状病毒污染的Sf9细胞株及其高效筛选方法 | |
| CN110358766A (zh) | 一种基于全基因组CRISPR/Cas9文库筛选异种移植抗原基因的方法 | |
| CN117051046B (zh) | 一种慢病毒载体及其应用 | |
| WO2023072178A1 (en) | Methods for developing a cell line for producing virus in suspension cell culture | |
| CN111154713A (zh) | 人胚肾上皮细胞293T-Clone3单克隆细胞株及其应用 | |
| CN102911911B (zh) | 人胚肾细胞293t-c10单克隆细胞株及其制备和应用 | |
| CN106916780B (zh) | 高密度悬浮培养的bhk21细胞克隆株 | |
| CN104894054A (zh) | 一种猴胚胎肾上皮细胞Marc-145悬浮适应株及其在培养蓝耳病病毒、生产蓝耳病毒疫苗中的应用 | |
| CN116694575B (zh) | 悬浮培养Marc145细胞的方法 | |
| CN119351341A (zh) | 基因工程化的成纤维细胞及其制备方法、增殖和/或分化b细胞的培养基与应用 | |
| CN114250193A (zh) | 一种人胚胎肾细胞系及其应用 | |
| CN108531442B (zh) | 一种无血清悬浮培养的昆虫细胞系及其应用 | |
| US20250188494A1 (en) | A method of cell electroporation | |
| CN116536251A (zh) | 一种无饲养层的化学诱导多能干细胞单克隆建株方法 | |
| CN116515728A (zh) | 无弹状病毒的Sf9-PT细胞及其应用 | |
| CN102453697A (zh) | 悬浮培养Vero细胞及利用其生产病毒疫苗的方法 | |
| CN113322234A (zh) | 永生化人cd34+cd38-造血干细胞系的制备方法及应用 | |
| CN116064371A (zh) | 犏牛肾细胞系及其应用 |