CN118660958A - 适应无血清悬浮培养的hek293细胞株及其应用 - Google Patents

适应无血清悬浮培养的hek293细胞株及其应用 Download PDF

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Publication number
CN118660958A
CN118660958A CN202280068776.XA CN202280068776A CN118660958A CN 118660958 A CN118660958 A CN 118660958A CN 202280068776 A CN202280068776 A CN 202280068776A CN 118660958 A CN118660958 A CN 118660958A
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cells
cell
medium
culture
serum
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Chinese (zh)
Inventor
王燕
潘晓
丁容娜
惠二京
宣春玲
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Jiangsu Jinsirui Biotech Co ltd
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Jiangsu Jinsirui Biotech Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C12N2750/14152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202280068776.XA 2021-10-12 2022-10-12 适应无血清悬浮培养的hek293细胞株及其应用 Pending CN118660958A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN202111190063 2021-10-12
CN2021111900634 2021-10-12
PCT/CN2022/124873 WO2023061409A1 (zh) 2021-10-12 2022-10-12 适应无血清悬浮培养的hek293细胞株及其应用

Publications (1)

Publication Number Publication Date
CN118660958A true CN118660958A (zh) 2024-09-17

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Application Number Title Priority Date Filing Date
CN202280068776.XA Pending CN118660958A (zh) 2021-10-12 2022-10-12 适应无血清悬浮培养的hek293细胞株及其应用

Country Status (6)

Country Link
US (1) US20240425880A1 (https=)
EP (1) EP4417689A4 (https=)
JP (1) JP2024538792A (https=)
KR (1) KR20240070587A (https=)
CN (1) CN118660958A (https=)
WO (1) WO2023061409A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121586778A (zh) * 2023-04-26 2026-02-27 赛诺菲 宿主细胞系以及用于鉴定和使用此类宿主细胞系的方法
CN117089514A (zh) * 2023-10-13 2023-11-21 思鹏生物科技(苏州)有限公司 提升hek293细胞系aav生产效率的细胞筛选驯化方法
CN117402817B (zh) * 2023-10-31 2024-09-27 山东丽山生物科技有限公司 一种无血清培养驯化贴壁293t细胞的方法及其应用
WO2025171001A1 (en) 2024-02-06 2025-08-14 Regeneron Pharmaceuticals, Inc. Compositions and methods for improved production of aav viral vectors
CN118222630B (zh) * 2024-05-23 2024-08-27 江西赛基生物技术有限公司 一种稳转表达cd16跨膜蛋白细胞系的构建方法及应用
CN119639654B (zh) * 2024-12-24 2025-10-31 华元(上海)生物医药有限公司 一种人胚胎肾单克隆细胞株及其在生产慢病毒载体中的应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2782997T (pt) * 2011-11-24 2018-04-09 Genethon Sistema de produção de vetor lentiviral escalável compatível com aplicações farmacêuticas industriais
CN102604889B (zh) * 2012-03-19 2013-05-08 中国农业科学院哈尔滨兽医研究所 适应无血清培养的hek293细胞系及其应用
RU2709678C2 (ru) * 2013-03-15 2019-12-19 Дзе Чилдрен'З Хоспитал Оф Филадельфия Масштабный производственный способ получения рекомбинантных лентивирусных векторов в системе культивирования клеток в бессывороточной суспензии
CN104450607B (zh) * 2014-11-14 2017-07-11 武汉金开瑞生物工程有限公司 用于hek293细胞悬浮生长的全化学成分培养基及培养方法
CN110713971B (zh) * 2019-11-06 2021-08-27 深圳市菲鹏生物治疗股份有限公司 一种无血清悬浮培养型293t细胞、其制备方法以及病毒的包装方法
KR20230126214A (ko) * 2020-12-29 2023-08-29 지앙수 젠스크립트 프로바이오 바이오테크 컴퍼니 리미티드 고분산성 hek293t 세포주 및 이의 스크리닝 방법

Also Published As

Publication number Publication date
US20240425880A1 (en) 2024-12-26
EP4417689A4 (en) 2025-10-15
KR20240070587A (ko) 2024-05-21
WO2023061409A1 (zh) 2023-04-20
JP2024538792A (ja) 2024-10-23
EP4417689A1 (en) 2024-08-21

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