JP2022540849A5 - - Google Patents

Info

Publication number
JP2022540849A5
JP2022540849A5 JP2022501200A JP2022501200A JP2022540849A5 JP 2022540849 A5 JP2022540849 A5 JP 2022540849A5 JP 2022501200 A JP2022501200 A JP 2022501200A JP 2022501200 A JP2022501200 A JP 2022501200A JP 2022540849 A5 JP2022540849 A5 JP 2022540849A5
Authority
JP
Japan
Prior art keywords
sulfotransferase
sulfo group
enzyme
modified
glucosaminyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2022501200A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022540849A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2020/041404 external-priority patent/WO2021007429A1/en
Publication of JP2022540849A publication Critical patent/JP2022540849A/ja
Publication of JP2022540849A5 publication Critical patent/JP2022540849A5/ja
Pending legal-status Critical Current

Links

JP2022501200A 2019-07-09 2020-07-09 抗凝固性多糖類の合成方法 Pending JP2022540849A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962871980P 2019-07-09 2019-07-09
US62/871,980 2019-07-09
US202063033687P 2020-06-02 2020-06-02
US63/033,687 2020-06-02
PCT/US2020/041404 WO2021007429A1 (en) 2019-07-09 2020-07-09 Methods for synthesizing anticoagulant polysaccharides

Publications (2)

Publication Number Publication Date
JP2022540849A JP2022540849A (ja) 2022-09-20
JP2022540849A5 true JP2022540849A5 (enExample) 2023-07-12

Family

ID=72046997

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022501200A Pending JP2022540849A (ja) 2019-07-09 2020-07-09 抗凝固性多糖類の合成方法

Country Status (9)

Country Link
US (3) US11542534B2 (enExample)
EP (1) EP3997238A1 (enExample)
JP (1) JP2022540849A (enExample)
CN (1) CN114616340A (enExample)
AU (1) AU2020310172A1 (enExample)
BR (1) BR112022000255A8 (enExample)
CA (1) CA3144968A1 (enExample)
MX (1) MX2022000343A (enExample)
WO (1) WO2021007429A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022015794A1 (en) 2020-07-14 2022-01-20 Optimvia, Llc Methods for synthesizing non-anticoagulant heparan sulfate
CN116284499B (zh) * 2022-07-28 2024-07-23 河北常山生化药业股份有限公司 一种羊源低分子肝素钠的制备方法
CN116120484B (zh) * 2022-09-01 2024-11-08 山东大学 一种抗凝血肝素寡糖苯联二聚体及其制备方法与应用
WO2025193841A1 (en) * 2024-03-13 2025-09-18 The Trustees Of Boston College Polysaccharides and methods of preparing same
WO2026116455A1 (ja) * 2024-11-29 2026-06-04 キリンバイオマテリアル株式会社 Ｎ－スルホトランスフェラーゼ活性を有する蛋白質及び該蛋白質をコードするｄｎａ

Family Cites Families (96)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3766167A (en) 1971-03-26 1973-10-16 Research Corp Orally active anticoagulant
DE2426586A1 (de) 1973-06-14 1975-01-09 Opocrin Srl Verfahren zur herstellung von glukuronil-glukosamin-glykan-sulfaten mit antilipaemischer wirkung
IT1083903B (it) 1977-08-09 1985-05-25 Lobo Srl Oligo-eteropolisaccaridi con attivita' eparinosimili,procedimento per il loro ottenimento e relative composizioni terapeutiche
FR2478646A2 (fr) 1980-03-20 1981-09-25 Choay Sa Composition mucopolysaccharidique ayant une activite regulatrice de la coagulation, medicament la contenant et procede pour l'obtenir
US4500519A (en) 1978-11-06 1985-02-19 Choay S.A. Mucopolysaccharides having biological properties, preparation and method of use
FR2440376A1 (fr) 1978-11-06 1980-05-30 Choay Sa Composition mucopolysaccharidique ayant une activite regulatrice de la coagulation, medicament la contenant et procede pour l'obtenir
US4692435A (en) 1978-11-06 1987-09-08 Choay, S.A. Mucopolysaccharide composition having a regulatory action on coagulation, medicament containing same and process of preparation
USRE35770E (en) 1978-11-06 1998-04-14 Choay, S.A. Oligosaccharides having anti-Xa activity and pharmaceutical compositions containing them
CA1136620A (en) 1979-01-08 1982-11-30 Ulf P.F. Lindahl Heparin fragments having selective anticoagulation activity
GB2051103A (en) 1979-04-11 1981-01-14 Choay Sa Heparin fractions
IL61201A (en) 1979-10-05 1984-09-30 Choay Sa Oligosaccharides having no more than 8 saccharide moieties,their obtention from heparin and pharmaceutical compositions containing them
US4826827A (en) 1979-10-05 1989-05-02 Choay S.A. Short chained oligosaccharides having biological properties, a process for making the same and the use thereof as drugs
US4281108A (en) 1980-01-28 1981-07-28 Hepar Industries, Inc. Process for obtaining low molecular weight heparins endowed with elevated pharmacological properties, and product so obtained
FR2482611B1 (fr) 1980-05-14 1986-03-07 Pharmindustrie Nouveaux polysaccharides sulfates, procedes pour leur preparation et leur utilisation comme medicaments
FR2482603A1 (fr) 1980-05-14 1981-11-20 Pharmindustrie Nouveaux esters d'heparine utilisables pour la preparation de medicaments, et procedes pour leur preparation
US4438261A (en) 1980-05-19 1984-03-20 Riker Laboratories, Inc. Anticoagulant substance
US4351938A (en) 1980-05-19 1982-09-28 Riker Laboratories, Inc. Anticoagulant substance
US4554101A (en) 1981-01-09 1985-11-19 New York Blood Center, Inc. Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity
FR2503714B1 (fr) 1981-04-10 1986-11-21 Choay Sa Procede d'obtention de mucopolysaccharides biologiquement actifs, de purete elevee, par depolymerisation de l'heparine
FR2504928A1 (fr) 1981-04-29 1982-11-05 Choay Sa Oligosaccharides a chaines courtes possedant des proprietes biologiques, leur preparation et leurs applications en tant que medicaments
US4396762A (en) 1981-08-24 1983-08-02 Massachusetts Institute Of Technology Heparinase derived anticoagulants
CA1195322A (en) 1982-07-19 1985-10-15 Eduardo Amaya Process for manufacturing low molecular weight heparins by depolymerization of normal heparin
US4533549A (en) 1983-01-04 1985-08-06 Lasker Sigmund E Antithrombotic agent
IT1195497B (it) 1983-03-08 1988-10-19 Opocrin Spa Procedimento per la preparazione di frazioni oligosaccaridiche dotate di proprieta' farmacologiche per degradazione chimica di eparina
US4686388A (en) 1985-03-12 1987-08-11 Pitney Bowes Inc. Integrated circuit substrate bias selection circuit
DK196986D0 (da) 1986-04-30 1986-04-30 Novo Industri As Fremstilling af polysaccharider
US5106734A (en) 1986-04-30 1992-04-21 Novo Nordisk A/S Process of using light absorption to control enzymatic depolymerization of heparin to produce low molecular weight heparin
DK196886D0 (da) 1986-04-30 1986-04-30 Novo Industri As Fremstilling af polysaccharider
IT1213384B (it) 1986-11-24 1989-12-20 Lab Derivati Organici Mediolan Processo per la preparazione controllata di gilcosaminoglicani a basso peso molecolare.
US4833155A (en) 1986-11-25 1989-05-23 Syntex (U.S.A.) Inc. 3-(ω-(3,5,-di-t-butyl-4-hydroxyphenyl)-alkanoyl)pyrroles, and anti-inflammatory uses thereof
ES2003197A6 (es) 1987-01-05 1988-10-16 Rovi Lab Farmaceut Sa Procedimiento de despolimerizacion de la heparina para la obtencion de una heparina de bajo peso molecular dotada de actividad antitrombotica
FR2614026B1 (fr) 1987-04-16 1992-04-17 Sanofi Sa Heparines de bas poids moleculaire, a structure reguliere, leur preparation et leurs applications biologiques
EP0337327A1 (en) 1988-04-09 1989-10-18 Bioiberica, S.A. Process for the preparation of new oligosaccharide fractions by controlled chemical depolimerization of heparin
IT1234508B (it) 1988-06-10 1992-05-19 Alfa Wassermann Spa Derivati eparinici e procedimento per la loro preparazione
US4981955A (en) 1988-06-28 1991-01-01 Lopez Lorenzo L Depolymerization method of heparin
IT1234826B (it) 1989-01-30 1992-05-29 Alfa Wassermann Spa Derivati eparinici e procedimento per la loro preparazione
IE64121B1 (en) 1989-10-04 1995-07-12 Akzo Nv Sulphated glycosaminoglycuronan with antithrombotic activity
US5378829A (en) 1990-04-23 1995-01-03 Akzo N.V. Sulfated glycosaminoglycanoid derivatives of the heparin and heparan sulfate type
USRE38743E1 (en) 1990-06-26 2005-06-14 Aventis Pharma S.A. Mixtures of particular LMW heparinic polysaccharides for the prophylaxis/treatment of acute thrombotic events
FR2663639B1 (fr) 1990-06-26 1994-03-18 Rhone Poulenc Sante Melanges de polysaccharides de bas poids moleculaires procede de preparation et utilisation.
US5821223A (en) 1990-09-14 1998-10-13 The United States Of America As Represented By The Department Of Health And Human Services Method of stimulating cell growth with a novel broad spectrum human lung fibroblast-derived mitogen
US5280016A (en) 1991-03-29 1994-01-18 Glycomed Incorporated Non-anticoagulant heparin derivatives
US5849721A (en) 1992-02-07 1998-12-15 Rhone-Poulenc Rorer S.A. Sulfated polysaccharides obtained from heparin, preparation process, pharmaceutical composition and use thereof
US5541095A (en) 1992-06-16 1996-07-30 University Of Massachusetts Medical Center Glycosaminoglycan specific sulfotransferases
FR2704861B1 (fr) 1993-05-07 1995-07-28 Sanofi Elf Fractions d'héparine purifiées, procédé d'obtention et compositions pharmaceutiques les contenant.
JP3818676B2 (ja) 1994-07-22 2006-09-06 生化学工業株式会社 ヘパラン硫酸６−ｏ−硫酸基転移酵素
US5919673A (en) 1995-03-22 1999-07-06 The Scripps Research Institute One-pot enzymatic sulfation process using 3'-phosphoadenosine-5'-phosphosulfate and recycled phosphorylated adenosine intermediates
JP3672359B2 (ja) 1995-07-24 2005-07-20 生化学工業株式会社 ヘパラン硫酸 ２−ｏ−硫酸基転移酵素
WO1999022005A2 (en) * 1997-10-24 1999-05-06 Massachusetts Institute Of Technology Heparan sulfate d-glucosaminyl 3-o-sulfotransferases, an uses therefor
US6861254B1 (en) 1997-10-24 2005-03-01 Massachusetts Institute Of Technology Heparan sulfate D-glucosaminyl 3-O-sulfotransferases, and uses therefor
US8088604B2 (en) 1998-04-02 2012-01-03 The Board Of Regents Of The University Of Oklahoma Production of defined monodisperse heparosan polymers and unnatural polymers with polysaccharide synthases
US7307159B2 (en) 2001-05-08 2007-12-11 The Board Of Regents Of The University Of Oklahoma Heparin/heparosan synthase from P. multocida and methods of making and using same
US20060188966A1 (en) 1998-04-02 2006-08-24 Deangelis Paul L Natural, chimeric and hybrid glycosaminoglycan polymers and methods of making and using same
DE69934890D1 (de) 1998-06-16 2007-03-08 Univ Oklahoma Glykosulfopeptide und verfahren zu deren herstellung und deren verwendung
US6255088B1 (en) 1999-05-11 2001-07-03 The Scripps Research Institute Enzymatic sulfation of biomolecules
DE10002005A1 (de) 2000-01-19 2001-08-09 Bosch Gmbh Robert Zerstäubungsdüse
CN1311305A (zh) * 2000-03-02 2001-09-05 上海博德基因开发有限公司 一种新的多肽——硫酸乙酰肝素3－0－磺基转移酶19和编码这种多肽的多核苷酸
WO2002023190A2 (en) 2000-09-12 2002-03-21 Massachusetts Institute Of Technology Methods and products related to low molecular weight heparin
FR2814463B1 (fr) 2000-09-22 2002-11-15 Sanofi Synthelabo Nouveaux polysaccharides a activite antithrombotique comprenant au moins une liaison covalente avec la biotine ou un derive de la biotine
ITMI20010397A1 (it) 2001-02-27 2002-08-27 Giorgio Zoppetti Derivati altamente n,o-solfatati del polisaccaride k5 e loro preparazione
CA2441984A1 (en) 2001-03-28 2002-10-10 Massachusetts Institute Of Technology Methods of 6-o-sulfating polysaccharides and 6-o-sulfated polysaccharide preparations
US8580290B2 (en) 2001-05-08 2013-11-12 The Board Of Regents Of The University Of Oklahoma Heparosan-based biomaterials and coatings and methods of production and use thereof
TWI281861B (en) 2002-01-29 2007-06-01 Sanofi Aventis A pharmaceutical composition comprising SANORG 34006 for treatment and secondary prophylaxis of venous thromboembolic events in patients with deep venous thrombosis
WO2004005475A2 (en) 2002-07-05 2004-01-15 University Of North Carolina At Chapel Hill Purified and isolated heparan sulfate 3-o-sulfotransferase isoform 5 nucleic acids and polypeptides and therapeutic and screening methods using same
AU2003262794A1 (en) * 2002-08-23 2004-03-11 Neose Technologies, Inc. Total synthesis of heparin
US20040147469A1 (en) 2002-11-01 2004-07-29 Case Western Reserve University Methods of inhibiting glial scar formation
WO2004050673A2 (en) 2002-11-27 2004-06-17 Massachusetts Institute Of Technology Methods for synthesizing polysaccharides
US7655445B2 (en) * 2003-11-12 2010-02-02 Massachusetts Institute Of Technology Methods for synthesis of sulfated saccharides
US8450297B2 (en) 2004-08-16 2013-05-28 Massachusetts Institute Of Technology Rapid two-step synthesis of anti-coagulants
WO2006083328A2 (en) 2004-09-15 2006-08-10 Massachusetts Institute Of Technology Biologically active surfaces and methods of their use
WO2006076627A2 (en) 2005-01-12 2006-07-20 Massachusetts Institute Of Technology Methods and compositions related to modulating the extracellular stem cell environment
EP1885865A4 (en) 2005-05-12 2010-12-22 Univ North Carolina ENZYMATIC SYNTHESIS OF SULFATED POLYSACCHARIDES
BRPI0612687B1 (pt) 2005-07-05 2019-05-14 Huawei Technologies Co., Ltd. Método de autenticação em subsistema multimídia ip
AU2007267561B2 (en) 2006-05-25 2012-05-17 Momenta Pharmaceuticals, Inc. Low molecular weight heparin composition and uses thereof
US20090035787A1 (en) 2007-07-23 2009-02-05 The University Of North Carolina At Chapel Hill Enzymatic synthesis of sulfated polysaccharides without iduronic acid residues
SG185925A1 (en) 2007-11-02 2012-12-28 Momenta Pharmaceuticals Inc Non-anticoagulant polysaccharide compositions
EP2205642B1 (en) 2007-11-02 2016-01-27 Momenta Pharmaceuticals, Inc. Non-anticoagulant polysaccharide compositions
EP2307440A4 (en) 2008-07-01 2012-12-19 Zacharon Pharmaceuticals Inc HEPARAN SULFATE INHIBITORS
WO2011014793A2 (en) 2009-07-31 2011-02-03 Reliable Biopharmaceutical Corporation Process for preparing fondaparinux sodium and intermediates useful in the synthesis thereof
CN101724086B (zh) 2009-11-25 2012-09-26 深圳海王药业有限公司 低聚凤梨参糖胺聚糖及其制备方法
WO2012088416A2 (en) * 2010-12-23 2012-06-28 The University Of North Carolina At Chapel Hill Chemoenzymatic synthesis of structurally homogeneous ultra-low molecular weight heparins
WO2012116048A1 (en) 2011-02-22 2012-08-30 Rensselaer Polytechnic Institute Single step heparosan n-deacetylation and depolymerization for making bioengineered heparin
US8815529B2 (en) 2011-05-23 2014-08-26 Research & Diagnostics Systems, Inc. Sulfotransferase assay
EP2831237B1 (en) 2012-03-30 2017-11-29 The Board of Regents of the University of Oklahoma High molecular weight heparosan polymers and methods of production and use thereof
CN102660610A (zh) * 2012-05-31 2012-09-12 江南大学 一种酶法制备高活性低分子量肝素的方法
US20140349962A1 (en) 2013-05-24 2014-11-27 Massachusetts Institute Of Technology Rapid two-step synthesis of anti-coagulants
JP6670235B2 (ja) 2013-06-17 2020-03-18 ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒルＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｎｏｒｔｈ Ｃａｒｏｌｉｎａ Ａｔ Ｃｈａｐｅｌ Ｈｉｌｌ 可逆的ヘパリン分子、その製法及びその使用方法
PL3183355T3 (pl) 2014-08-22 2020-06-15 Cysbio Aps Biologiczny sposób wytwarzania siarczanowanego związku fenolowego
EP3399044B1 (en) * 2015-12-28 2021-02-03 Ajinomoto Co., Inc. Method for producing heparan sulfate having anticoagulant activity
EP3420095A1 (en) 2016-02-24 2019-01-02 Danmarks Tekniske Universitet Improved biological processes for the production of aryl sulfates
TWI769176B (zh) 2016-09-07 2022-07-01 瑞瑟勒綜合技術協會 生合成肝素
EP3572522A4 (en) 2017-01-19 2020-10-28 Ajinomoto Co., Inc. PROCESS FOR THE PRODUCTION OF A HEPAROSANE COMPOUND CONTAINING A RESIDUE OF ISOMERIZED HEXULONIC ACID
WO2018165656A1 (en) 2017-03-10 2018-09-13 The University Of North Carolina At Chapel Hill Short-acting heparin-based anticoagulant compounds and methods
JP7200948B2 (ja) 2017-09-05 2023-01-10 味の素株式会社 ２－ｏ－硫酸化酵素変異体、および３－ｏ－硫酸化酵素変異体、ならびにそれらを用いる方法
CN108220365A (zh) * 2018-01-16 2018-06-29 浙江海洋大学 一种提高肝素抗凝血活性的方法
CA3126805A1 (en) * 2019-01-15 2020-07-23 Optimvia, Llc Engineered aryl sulfate-dependent enzymes

Similar Documents

Publication Publication Date Title
EP0789777B1 (en) Polysaccharides having a high iduronic acid content
Chen et al. Tailor design and synthesis of heparan sulfate (HS) oligosaccharide analogs using sequential one-pot multienzyme (OPME) systems
Karst et al. Recent chemical and enzymatic approaches to the synthesis of glycosaminoglycan oligosaccharides
Chavaroche et al. Production methods for heparosan, a precursor of heparin and heparan sulfate
DeAngelis et al. Identification and molecular cloning of a chondroitin synthase from Pasteurella multocida type F
Pettersson et al. Biosynthesis of heparin. Purification of a 110-kDa mouse mastocytoma protein required for both glucosaminyl N-deacetylation and N-sulfation
Casu Heparin structure
Lidholt et al. Biosynthesis of heparin. Relationship between the polymerization and sulphation processes
Douaisi et al. Synthesis of bioengineered heparin chemically and biologically similar to porcine-derived products and convertible to low MW heparin
Kusche et al. Biosynthesis of heparin. Use of Escherichia coli K5 capsular polysaccharide as a model substrate in enzymic polymer-modification reactions
Naggi et al. Toward a biotechnological heparin through combined chemical and enzymatic modification of the Escherichia coli K5 polysaccharide
HUT67208A (en) Anticoagulants and processes for preparing such
Bame et al. Biosynthesis of heparan sulfate. Coordination of polymer-modification reactions in a Chinese hamster ovary cell mutant defective in N-sulfotransferase
CZ279081B6 (en) N,o-sulfated heparosanes, process of their preparation and pharmaceutical compositions containing said n,o-sulfated heparosanes and a strain escherichia coli (k5) sebr 3282
Jin et al. Preactivation-based, iterative one-pot synthesis of anticoagulant pentasaccharide fondaparinux sodium
JP4267916B2 (ja) 多糖体ｋ５から誘導された高度のアンチトロンビン活性を有するグリコサミノグリカン及びその製法
EP0897393A1 (en) Derivatives of k5 polysaccharide having high anticoagulant activity
RU2361881C2 (ru) Производные полисахаридов с высокой антитромботической активностью в плазме
Kusche et al. Biosynthesis of heparin. O-sulfation of D-glucuronic acid units.
US7655445B2 (en) Methods for synthesis of sulfated saccharides
Rodén et al. Heparin—an introduction
Kusche-Gullberg et al. Drosophila heparan sulfate, a novel design
WO2024032575A1 (zh) 一种含双抗凝血酶结合序列的肝素十二糖及其制备方法与应用
US20090005341A1 (en) Process for the preparation of n-acyl-(epi)k5-amine-o-sulfate-derivatives and products thus obtained
EP1694714B1 (en) Low molecular weight polysaccharides having antithrombotic activity