BR112022000255A8 - Métodos para sintetizar polissacarídeos anticoagulantes - Google Patents

Métodos para sintetizar polissacarídeos anticoagulantes

Info

Publication number
BR112022000255A8
BR112022000255A8 BR112022000255A BR112022000255A BR112022000255A8 BR 112022000255 A8 BR112022000255 A8 BR 112022000255A8 BR 112022000255 A BR112022000255 A BR 112022000255A BR 112022000255 A BR112022000255 A BR 112022000255A BR 112022000255 A8 BR112022000255 A8 BR 112022000255A8
Authority
BR
Brazil
Prior art keywords
polysaccharides
anticoagulant
methods
synthesizing
sulfate
Prior art date
Application number
BR112022000255A
Other languages
English (en)
Other versions
BR112022000255A2 (pt
Inventor
H Lajiness Daniel
Ferreira Gesteira Tarsis
Original Assignee
Optimvia Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Optimvia Llc filed Critical Optimvia Llc
Publication of BR112022000255A2 publication Critical patent/BR112022000255A2/pt
Publication of BR112022000255A8 publication Critical patent/BR112022000255A8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
    • A61K31/727—Heparin; Heparan
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0069—Chondroitin-4-sulfate, i.e. chondroitin sulfate A; Dermatan sulfate, i.e. chondroitin sulfate B or beta-heparin; Chondroitin-6-sulfate, i.e. chondroitin sulfate C; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0075—Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C08L5/10—Heparin; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/02—Sulfotransferases (2.8.2)
    • C12Y208/02008—[Heparan sulfate]-glucosamine N-sulfotransferase (2.8.2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/02—Sulfotransferases (2.8.2)
    • C12Y208/02023—[Heparan sulfate]-glucosamine 3-sulfotransferase 1 (2.8.2.23)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03017—Heparosan-N-sulfate-glucuronate 5-epimerase (5.1.3.17)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
    • C08J2305/10—Heparin; Derivatives thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Materials Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Dermatology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Hematology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

métodos para sintetizar polissacarídeos anticoagulantes. a presente invenção inclui métodos para preparar polissacarídeos anticoagulantes usando várias enzimas sulfotransferases modificadas que não ocorrem naturalmente que são designadas para reagir com compostos de sulfato de arila ao invés do substrato natural, 5'-fosfossulfato de 3'-fosfoadenosina, para facilitar a transferência de grupo sulfo para polissacarídeos aceptores de grupo sulfo. os compostos de sulfato de arila adequados incluem, mas não são limitados a sulfato de p-nitrofenila ou sulfato de 4-nitrocatecol. polissacarídeos anticoagulantes produzidos pelos métodos da presente invenção compreendem resíduos de glucosamina n-, 3-o-, 6-o-sulfatada e resíduos de ácido hexurônico 2-o sulfatado, têm atividade anticoagulante comparável em comparação a polissacarídeos anticoagulantes comercialmente disponíveis, e podem ser utilizados para formar polissacarídeos anticoagulantes truncados tendo um peso molecular reduzido.
BR112022000255A 2019-07-09 2020-07-09 Métodos para sintetizar polissacarídeos anticoagulantes BR112022000255A8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962871980P 2019-07-09 2019-07-09
US202063033687P 2020-06-02 2020-06-02
PCT/US2020/041404 WO2021007429A1 (en) 2019-07-09 2020-07-09 Methods for synthesizing anticoagulant polysaccharides

Publications (2)

Publication Number Publication Date
BR112022000255A2 BR112022000255A2 (pt) 2022-03-03
BR112022000255A8 true BR112022000255A8 (pt) 2022-03-22

Family

ID=72046997

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022000255A BR112022000255A8 (pt) 2019-07-09 2020-07-09 Métodos para sintetizar polissacarídeos anticoagulantes

Country Status (9)

Country Link
US (3) US11542534B2 (pt)
EP (1) EP3997238A1 (pt)
JP (1) JP2022540849A (pt)
CN (1) CN114616340A (pt)
AU (1) AU2020310172A1 (pt)
BR (1) BR112022000255A8 (pt)
CA (1) CA3144968A1 (pt)
MX (1) MX2022000343A (pt)
WO (1) WO2021007429A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022015794A1 (en) 2020-07-14 2022-01-20 Optimvia, Llc Methods for synthesizing non-anticoagulant heparan sulfate

Family Cites Families (95)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3766167A (en) 1971-03-26 1973-10-16 Research Corp Orally active anticoagulant
DE2426586A1 (de) 1973-06-14 1975-01-09 Opocrin Srl Verfahren zur herstellung von glukuronil-glukosamin-glykan-sulfaten mit antilipaemischer wirkung
IT1083903B (it) 1977-08-09 1985-05-25 Lobo Srl Oligo-eteropolisaccaridi con attivita' eparinosimili,procedimento per il loro ottenimento e relative composizioni terapeutiche
FR2478646A2 (fr) 1980-03-20 1981-09-25 Choay Sa Composition mucopolysaccharidique ayant une activite regulatrice de la coagulation, medicament la contenant et procede pour l'obtenir
USRE35770E (en) 1978-11-06 1998-04-14 Choay, S.A. Oligosaccharides having anti-Xa activity and pharmaceutical compositions containing them
US4692435A (en) 1978-11-06 1987-09-08 Choay, S.A. Mucopolysaccharide composition having a regulatory action on coagulation, medicament containing same and process of preparation
FR2440376A1 (fr) 1978-11-06 1980-05-30 Choay Sa Composition mucopolysaccharidique ayant une activite regulatrice de la coagulation, medicament la contenant et procede pour l'obtenir
US4500519A (en) 1978-11-06 1985-02-19 Choay S.A. Mucopolysaccharides having biological properties, preparation and method of use
CA1136620A (en) 1979-01-08 1982-11-30 Ulf P.F. Lindahl Heparin fragments having selective anticoagulation activity
GB2051103A (en) 1979-04-11 1981-01-14 Choay Sa Heparin fractions
US4826827A (en) 1979-10-05 1989-05-02 Choay S.A. Short chained oligosaccharides having biological properties, a process for making the same and the use thereof as drugs
IL61201A (en) 1979-10-05 1984-09-30 Choay Sa Oligosaccharides having no more than 8 saccharide moieties,their obtention from heparin and pharmaceutical compositions containing them
US4281108A (en) 1980-01-28 1981-07-28 Hepar Industries, Inc. Process for obtaining low molecular weight heparins endowed with elevated pharmacological properties, and product so obtained
FR2482611B1 (fr) 1980-05-14 1986-03-07 Pharmindustrie Nouveaux polysaccharides sulfates, procedes pour leur preparation et leur utilisation comme medicaments
FR2482603A1 (fr) 1980-05-14 1981-11-20 Pharmindustrie Nouveaux esters d'heparine utilisables pour la preparation de medicaments, et procedes pour leur preparation
US4438261A (en) 1980-05-19 1984-03-20 Riker Laboratories, Inc. Anticoagulant substance
US4351938A (en) 1980-05-19 1982-09-28 Riker Laboratories, Inc. Anticoagulant substance
US4554101A (en) 1981-01-09 1985-11-19 New York Blood Center, Inc. Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity
FR2503714B1 (fr) 1981-04-10 1986-11-21 Choay Sa Procede d'obtention de mucopolysaccharides biologiquement actifs, de purete elevee, par depolymerisation de l'heparine
FR2504928A1 (fr) 1981-04-29 1982-11-05 Choay Sa Oligosaccharides a chaines courtes possedant des proprietes biologiques, leur preparation et leurs applications en tant que medicaments
US4396762A (en) 1981-08-24 1983-08-02 Massachusetts Institute Of Technology Heparinase derived anticoagulants
CA1195322A (en) 1982-07-19 1985-10-15 Eduardo Amaya Process for manufacturing low molecular weight heparins by depolymerization of normal heparin
US4533549A (en) 1983-01-04 1985-08-06 Lasker Sigmund E Antithrombotic agent
IT1195497B (it) 1983-03-08 1988-10-19 Opocrin Spa Procedimento per la preparazione di frazioni oligosaccaridiche dotate di proprieta' farmacologiche per degradazione chimica di eparina
US4686388A (en) 1985-03-12 1987-08-11 Pitney Bowes Inc. Integrated circuit substrate bias selection circuit
US5106734A (en) 1986-04-30 1992-04-21 Novo Nordisk A/S Process of using light absorption to control enzymatic depolymerization of heparin to produce low molecular weight heparin
DK196886D0 (da) 1986-04-30 1986-04-30 Novo Industri As Fremstilling af polysaccharider
DK196986D0 (da) 1986-04-30 1986-04-30 Novo Industri As Fremstilling af polysaccharider
IT1213384B (it) 1986-11-24 1989-12-20 Lab Derivati Organici Mediolan Processo per la preparazione controllata di gilcosaminoglicani a basso peso molecolare.
US4833155A (en) 1986-11-25 1989-05-23 Syntex (U.S.A.) Inc. 3-(ω-(3,5,-di-t-butyl-4-hydroxyphenyl)-alkanoyl)pyrroles, and anti-inflammatory uses thereof
ES2003197A6 (es) 1987-01-05 1988-10-16 Rovi Lab Farmaceut Sa Procedimiento de despolimerizacion de la heparina para la obtencion de una heparina de bajo peso molecular dotada de actividad antitrombotica
FR2614026B1 (fr) 1987-04-16 1992-04-17 Sanofi Sa Heparines de bas poids moleculaire, a structure reguliere, leur preparation et leurs applications biologiques
EP0337327A1 (en) 1988-04-09 1989-10-18 Bioiberica, S.A. Process for the preparation of new oligosaccharide fractions by controlled chemical depolimerization of heparin
IT1234508B (it) 1988-06-10 1992-05-19 Alfa Wassermann Spa Derivati eparinici e procedimento per la loro preparazione
US4981955A (en) 1988-06-28 1991-01-01 Lopez Lorenzo L Depolymerization method of heparin
IT1234826B (it) 1989-01-30 1992-05-29 Alfa Wassermann Spa Derivati eparinici e procedimento per la loro preparazione
IE64121B1 (en) 1989-10-04 1995-07-12 Akzo Nv Sulphated glycosaminoglycuronan with antithrombotic activity
US5378829A (en) 1990-04-23 1995-01-03 Akzo N.V. Sulfated glycosaminoglycanoid derivatives of the heparin and heparan sulfate type
FR2663639B1 (fr) 1990-06-26 1994-03-18 Rhone Poulenc Sante Melanges de polysaccharides de bas poids moleculaires procede de preparation et utilisation.
USRE38743E1 (en) 1990-06-26 2005-06-14 Aventis Pharma S.A. Mixtures of particular LMW heparinic polysaccharides for the prophylaxis/treatment of acute thrombotic events
US5821223A (en) 1990-09-14 1998-10-13 The United States Of America As Represented By The Department Of Health And Human Services Method of stimulating cell growth with a novel broad spectrum human lung fibroblast-derived mitogen
US5280016A (en) 1991-03-29 1994-01-18 Glycomed Incorporated Non-anticoagulant heparin derivatives
US5849721A (en) 1992-02-07 1998-12-15 Rhone-Poulenc Rorer S.A. Sulfated polysaccharides obtained from heparin, preparation process, pharmaceutical composition and use thereof
US5541095A (en) 1992-06-16 1996-07-30 University Of Massachusetts Medical Center Glycosaminoglycan specific sulfotransferases
FR2704861B1 (fr) 1993-05-07 1995-07-28 Sanofi Elf Fractions d'héparine purifiées, procédé d'obtention et compositions pharmaceutiques les contenant.
JP3818676B2 (ja) 1994-07-22 2006-09-06 生化学工業株式会社 ヘパラン硫酸６−ｏ−硫酸基転移酵素
US5919673A (en) 1995-03-22 1999-07-06 The Scripps Research Institute One-pot enzymatic sulfation process using 3'-phosphoadenosine-5'-phosphosulfate and recycled phosphorylated adenosine intermediates
JP3672359B2 (ja) 1995-07-24 2005-07-20 生化学工業株式会社 ヘパラン硫酸 ２−ｏ−硫酸基転移酵素
US6861254B1 (en) 1997-10-24 2005-03-01 Massachusetts Institute Of Technology Heparan sulfate D-glucosaminyl 3-O-sulfotransferases, and uses therefor
US20060188966A1 (en) 1998-04-02 2006-08-24 Deangelis Paul L Natural, chimeric and hybrid glycosaminoglycan polymers and methods of making and using same
US8088604B2 (en) 1998-04-02 2012-01-03 The Board Of Regents Of The University Of Oklahoma Production of defined monodisperse heparosan polymers and unnatural polymers with polysaccharide synthases
DE69934890D1 (de) 1998-06-16 2007-03-08 Univ Oklahoma Glykosulfopeptide und verfahren zu deren herstellung und deren verwendung
US6255088B1 (en) 1999-05-11 2001-07-03 The Scripps Research Institute Enzymatic sulfation of biomolecules
DE10002005A1 (de) 2000-01-19 2001-08-09 Bosch Gmbh Robert Zerstäubungsdüse
CN1311305A (zh) * 2000-03-02 2001-09-05 上海博德基因开发有限公司 一种新的多肽——硫酸乙酰肝素3－0－磺基转移酶19和编码这种多肽的多核苷酸
EP1319183B1 (en) 2000-09-12 2009-03-25 Massachusetts Institute Of Technology Methods and products related to low molecular weight heparin
FR2814463B1 (fr) 2000-09-22 2002-11-15 Sanofi Synthelabo Nouveaux polysaccharides a activite antithrombotique comprenant au moins une liaison covalente avec la biotine ou un derive de la biotine
ITMI20010397A1 (it) 2001-02-27 2002-08-27 Giorgio Zoppetti Derivati altamente n,o-solfatati del polisaccaride k5 e loro preparazione
CA2441984A1 (en) 2001-03-28 2002-10-10 Massachusetts Institute Of Technology Methods of 6-o-sulfating polysaccharides and 6-o-sulfated polysaccharide preparations
US8580290B2 (en) 2001-05-08 2013-11-12 The Board Of Regents Of The University Of Oklahoma Heparosan-based biomaterials and coatings and methods of production and use thereof
WO2002089742A2 (en) 2001-05-08 2002-11-14 The Board Of Regents Of The University Of Oklahoma Heparin/heparosan synthase and methods of making and using same
TWI281861B (en) 2002-01-29 2007-06-01 Sanofi Aventis A pharmaceutical composition comprising SANORG 34006 for treatment and secondary prophylaxis of venous thromboembolic events in patients with deep venous thrombosis
WO2004005475A2 (en) 2002-07-05 2004-01-15 University Of North Carolina At Chapel Hill Purified and isolated heparan sulfate 3-o-sulfotransferase isoform 5 nucleic acids and polypeptides and therapeutic and screening methods using same
WO2004017910A2 (en) * 2002-08-23 2004-03-04 Neose Technologies, Inc. Total synthesis of heparin
US20040147469A1 (en) 2002-11-01 2004-07-29 Case Western Reserve University Methods of inhibiting glial scar formation
AU2003293122A1 (en) 2002-11-27 2004-06-23 Massachusetts Institute Of Technology Methods for synthesizing polysaccharides
US7655445B2 (en) * 2003-11-12 2010-02-02 Massachusetts Institute Of Technology Methods for synthesis of sulfated saccharides
WO2006023487A2 (en) * 2004-08-16 2006-03-02 Massachusetts Institute Of Technology Rapid two-step synthesis of anti-coagulants
US20060154894A1 (en) 2004-09-15 2006-07-13 Massachusetts Institute Of Technology Biologically active surfaces and methods of their use
CA2594013A1 (en) 2005-01-12 2006-07-20 Massachusetts Institute Of Technology Methods and compositions related to modulating the extracellular stem cell environment
EP1885865A4 (en) 2005-05-12 2010-12-22 Univ North Carolina ENZYMATIC SYNTHESIS OF SULFATED POLYSACCHARIDES
EP1853032B1 (en) 2005-07-05 2009-12-23 Huawei Technologies Co., Ltd. An authentication method for the ip multimedia subsystem
CA2652205A1 (en) 2006-05-25 2007-12-06 Mallik Sundaram Low molecular weight heparin composition and uses thereof
US20090035787A1 (en) 2007-07-23 2009-02-05 The University Of North Carolina At Chapel Hill Enzymatic synthesis of sulfated polysaccharides without iduronic acid residues
EP2207811B1 (en) 2007-11-02 2017-01-04 Momenta Pharmaceuticals, Inc. Non-anticoagulant polysaccharide compositions
WO2009059284A2 (en) 2007-11-02 2009-05-07 Momenta Pharmaceuticals, Inc. Non-anticoagulant polysaccharide compositions
JP2011526925A (ja) 2008-07-01 2011-10-20 ザカロン ファーマシューティカルズ，インク． ヘパラン硫酸阻害剤
CN104245718B (zh) 2009-07-31 2016-11-09 可靠生物医药公司 制备磺达肝素钠的方法及其合成中使用的中间体
CN101724086B (zh) 2009-11-25 2012-09-26 深圳海王药业有限公司 低聚凤梨参糖胺聚糖及其制备方法
WO2012088416A2 (en) * 2010-12-23 2012-06-28 The University Of North Carolina At Chapel Hill Chemoenzymatic synthesis of structurally homogeneous ultra-low molecular weight heparins
WO2012116048A1 (en) 2011-02-22 2012-08-30 Rensselaer Polytechnic Institute Single step heparosan n-deacetylation and depolymerization for making bioengineered heparin
US8815529B2 (en) 2011-05-23 2014-08-26 Research & Diagnostics Systems, Inc. Sulfotransferase assay
WO2013149161A1 (en) 2012-03-30 2013-10-03 Deangelis Paul L High molecular weight heparosan polymers and methods of production and use thereof
CN102660610A (zh) * 2012-05-31 2012-09-12 江南大学 一种酶法制备高活性低分子量肝素的方法
US20140349962A1 (en) 2013-05-24 2014-11-27 Massachusetts Institute Of Technology Rapid two-step synthesis of anti-coagulants
EP3011043B1 (en) 2013-06-17 2022-05-11 The University of North Carolina At Chapel Hill Reversible heparin molecules
US20170226543A1 (en) 2014-08-22 2017-08-10 Danmarks Tekniske Universitet Biological processes for the production of aryl sulfates
JP6879216B2 (ja) * 2015-12-28 2021-06-02 味の素株式会社 抗凝固活性を有するヘパラン硫酸の製造法
CN109072262A (zh) 2016-02-24 2018-12-21 丹麦技术大学 用于生产芳基硫酸酯的改进的生物方法
TWI769176B (zh) 2016-09-07 2022-07-01 瑞瑟勒綜合技術協會 生合成肝素
WO2018135027A1 (ja) 2017-01-19 2018-07-26 味の素株式会社 ヘキスロン酸残基が異性化されたヘパロサン化合物の製造方法
CN110446511B (zh) 2017-03-10 2024-04-02 北卡罗来纳大学查珀尔希尔分校 短效的基于肝素的抗凝血剂化合物和方法
JP7200948B2 (ja) 2017-09-05 2023-01-10 味の素株式会社 ２－ｏ－硫酸化酵素変異体、および３－ｏ－硫酸化酵素変異体、ならびにそれらを用いる方法
CN108220365A (zh) * 2018-01-16 2018-06-29 浙江海洋大学 一种提高肝素抗凝血活性的方法
EP3911737A1 (en) * 2019-01-15 2021-11-24 Optimvia, LLC Engineered aryl sulfate-dependent enzymes

Also Published As

Publication number Publication date
US20230013513A1 (en) 2023-01-19
BR112022000255A2 (pt) 2022-03-03
JP2022540849A (ja) 2022-09-20
MX2022000343A (es) 2022-03-11
US11542534B2 (en) 2023-01-03
EP3997238A1 (en) 2022-05-18
CA3144968A1 (en) 2021-01-14
US11629364B2 (en) 2023-04-18
CN114616340A (zh) 2022-06-10
AU2020310172A1 (en) 2022-03-03
US20230340552A1 (en) 2023-10-26
US20220010344A1 (en) 2022-01-13
WO2021007429A1 (en) 2021-01-14

Similar Documents

Publication Publication Date Title
Van Mooy et al. Sulfolipids dramatically decrease phosphorus demand by picocyanobacteria in oligotrophic marine environments
Levander Tissue induction
BRPI1105642A8 (pt) Métodos de preparação de composições de poliglicerila
BR112018008061A2 (pt) métodos para a cultura de células e kits e aparelhos para os mesmos
BR112018010635A2 (pt) linhagens celulares estáveis para a produção retroviral
BR112022001324A2 (pt) Composto, composição farmacêutica e método para tratamento de uma doença mediada por p38 map quinase em um indivíduo necessitando do mesmo
CN105452479A (zh) 可逆肝素分子
Otto et al. Structure/function analysis of Pasteurella multocida heparosan synthases: toward defining enzyme specificity and engineering novel catalysts
BR112022000255A2 (pt) Métodos para sintetizar polissacarídeos anticoagulantes
BR112018007960A2 (pt) conjugado, proteína pcrv, composição imunogênica, métodos de preparação de uma composição imunogênica, de tratamento e de produção de um bioconjugado, polinucleotídeo, vetor, célula hospedeira, e, bioconjugado.
Zhang et al. “Coding” and “Decoding”: hypothesis for the regulatory mechanism involved in heparan sulfate biosynthesis
BR112021024063A2 (pt) Identificação e seleção de um material de partida vegetal de sulfato de condroitina vegetal e ácido hialurônico, e transformação de tal material de partida vegetal para obter ingredientes para uso em alimentos, suplementos, dispositivos médicos ou fármacos
Jønsson et al. Systematics and biogeography of Indo-Pacific ground-doves
MX2021008479A (es) Enzimas dependientes de aril sulfato modificadas por ingenieria genetica.
BR112021026002A2 (pt) Regeneração e transformação melhorada de plantas com a ajuda de um gene de reforço grf1
BR112015007410A2 (pt) processo para a preparação de (co)polímeros de dienos conjugados na presença de um sistema catalítico que compreende um complexo de oxo-nitrogenado de cobalto
BR112016015875A2 (pt) Elementos intensificadores de cpmv
BR112016021298A2 (pt) Composições e métodos para produzir produtos químicos e derivados dos mesmos
Robinson A study of the adenosinetriphosphatase activity of developing chick muscle
Goff Ben Sira and the giants of the land: a note on Ben Sira 16: 7
BR112018075021A2 (pt) composição cosmética capilar
BR112021023242A2 (pt) Método para cultura de cartilagem e esferoides da mesma
BR112018017204A2 (pt) método para detectar ou separar/obter célula tumoral circulante empregando método de proliferação celular
BR112021023282A2 (pt) Composto, método para preparar um composto de fórmula química 1, e, composição farmacêutica
Mendenhall The message of Abdi-ashirta to the warriors, EA 74