BR112018017204A2 - método para detectar ou separar/obter célula tumoral circulante empregando método de proliferação celular - Google Patents

método para detectar ou separar/obter célula tumoral circulante empregando método de proliferação celular

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Publication number
BR112018017204A2
BR112018017204A2 BR112018017204A BR112018017204A BR112018017204A2 BR 112018017204 A2 BR112018017204 A2 BR 112018017204A2 BR 112018017204 A BR112018017204 A BR 112018017204A BR 112018017204 A BR112018017204 A BR 112018017204A BR 112018017204 A2 BR112018017204 A2 BR 112018017204A2
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Brazil
Prior art keywords
separating
detecting
circulating
obtaining
circulating tumor
Prior art date
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BR112018017204A
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English (en)
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Umezu Yasuiki
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Umezu Yasuiki
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Publication date
Application filed by Umezu Yasuiki filed Critical Umezu Yasuiki
Publication of BR112018017204A2 publication Critical patent/BR112018017204A2/pt

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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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    • G01N33/483—Physical analysis of biological material
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
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    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
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  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

a presente invenção refere-se a um método para detectar ou separar / obter ctc, que é capaz de detectar e separar/obter de forma fiável e estável uma célula tumoral circulante e uma célula tronco tumoral circulante presente em quantidades vestigiais em um fluido circulante biológico tal como sangue ou linfa, mesmo no estado em que o tipo de câncer das células tumorais não pode ser determinado ainda e no estado em que as células tumorais estão presentes em quantidades vestigiais no fluido circulante biológico. o objetivo é atingido por um método para detectar ou separar/obter ctc e/ou ctsc em um fluido circulante biológico, compreendendo as seguintes etapas de tratamento (1) a (4): (1) uma primeira etapa de pré-tratar uma amostra a partir do fluido circulante biológico para obter uma fase celular mononuclear; (2) uma segunda etapa de proporcionar uma placa de poço na qual foi injetado um meio de cultura consistindo em um meio de crescimento celular livre de soro para células tumorais circulantes e/ou células tronco tumorais circulantes e semear as células mononucleares obtidas na primeira etapa, seguido de incubação; (3) uma terceira etapa de remover o meio de cultura de um poço da placa obtido pela incubação na segunda etapa; e (4) uma quarta etapa de detectar ou separar/obter uma célula tumoral aderente ligada ao poço da placa após a terceira etapa.
BR112018017204A 2016-03-03 2016-03-03 método para detectar ou separar/obter célula tumoral circulante empregando método de proliferação celular BR112018017204A2 (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2016/001172 WO2017149564A1 (ja) 2016-03-03 2016-03-03 細胞増殖法を用いた循環腫瘍細胞の検出・分離取得方法

Publications (1)

Publication Number Publication Date
BR112018017204A2 true BR112018017204A2 (pt) 2019-01-02

Family

ID=59505194

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018017204A BR112018017204A2 (pt) 2016-03-03 2016-03-03 método para detectar ou separar/obter célula tumoral circulante empregando método de proliferação celular

Country Status (14)

Country Link
US (1) US20190049456A1 (pt)
EP (1) EP3406714A4 (pt)
JP (1) JP6173577B1 (pt)
KR (1) KR20180114209A (pt)
CN (1) CN109073626A (pt)
AU (1) AU2016395556B2 (pt)
BR (1) BR112018017204A2 (pt)
CA (1) CA3049519A1 (pt)
IL (1) IL261398B (pt)
NZ (1) NZ745735A (pt)
RU (1) RU2707083C1 (pt)
SG (1) SG11201807509PA (pt)
TW (1) TWI618931B (pt)
WO (1) WO2017149564A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021188578A1 (en) * 2020-03-16 2021-09-23 University Of Southern California Screening methods to identify small molecule compounds that promote or inhibit the growth of circulating tumor cells, and uses thereof
CN113088494B (zh) * 2021-04-13 2022-08-05 武汉大学 一种用于释放被红细胞仿生材料捕获的肿瘤细胞的方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030190638A1 (en) * 1992-05-13 2003-10-09 Board Of Regents, The University Of Texas System Methods of screening for compounds that derepress or increase telomerase activity
AU6128794A (en) * 1993-01-27 1994-08-15 Batle Luminetrics Enterprises, Inc. Methods and kits for determining effects of anti-cancer agents on cancer cells
CN1153064C (zh) * 1996-04-05 2004-06-09 约翰斯·霍普金斯大学 富集稀少细胞的方法
JP5343092B2 (ja) * 2008-01-29 2013-11-13 カリフォルニア インスティチュート オブ テクノロジー 細胞分離を行う精密濾過の方法及び装置
US20130196349A1 (en) * 2011-08-02 2013-08-01 Realbio Technology, Inc. In Vitro Tumor Metastasis Model
MX2014014245A (es) * 2012-05-24 2015-06-17 Rarecells Proceso para multi-analisis de celulas raras extraidas o aisladas de muestras biologicas por filtracion.
CN103571792B (zh) * 2012-07-25 2015-11-18 中国科学院大连化学物理研究所 一种体外扩增肿瘤干细胞的方法
CN103352027A (zh) * 2013-05-07 2013-10-16 中国人民解放军第二军医大学 肿瘤干细胞的悬浮培养方法
CN104745531B (zh) * 2015-02-13 2017-10-27 河南大学 一种构建肿瘤多药耐药细胞模型的方法及通过该法建立的人乳腺癌多药耐药细胞株

Also Published As

Publication number Publication date
TW201732291A (zh) 2017-09-16
EP3406714A1 (en) 2018-11-28
US20190049456A1 (en) 2019-02-14
JPWO2017149564A1 (ja) 2018-03-08
KR20180114209A (ko) 2018-10-17
TWI618931B (zh) 2018-03-21
JP6173577B1 (ja) 2017-08-02
AU2016395556B2 (en) 2018-10-04
AU2016395556A1 (en) 2018-09-13
IL261398A (en) 2018-10-31
IL261398B (en) 2019-06-30
CN109073626A (zh) 2018-12-21
RU2707083C1 (ru) 2019-11-22
CA3049519A1 (en) 2017-09-08
SG11201807509PA (en) 2018-09-27
WO2017149564A1 (ja) 2017-09-08
NZ745735A (en) 2020-01-31
EP3406714A4 (en) 2018-11-28

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2660 DE 28-12-2021 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.