BR112017011759A2 - métodos para produzir um produto de interesse com células microbianas, para degradar dna e para romper uma célula microbiana. - Google Patents

métodos para produzir um produto de interesse com células microbianas, para degradar dna e para romper uma célula microbiana.

Info

Publication number
BR112017011759A2
BR112017011759A2 BR112017011759A BR112017011759A BR112017011759A2 BR 112017011759 A2 BR112017011759 A2 BR 112017011759A2 BR 112017011759 A BR112017011759 A BR 112017011759A BR 112017011759 A BR112017011759 A BR 112017011759A BR 112017011759 A2 BR112017011759 A2 BR 112017011759A2
Authority
BR
Brazil
Prior art keywords
microbial
producing
product
disrupting
interest
Prior art date
Application number
BR112017011759A
Other languages
English (en)
Inventor
Hoff Birgit
Scholten Edzard
Haefner Stefan
Kyu Jeong Weol
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Se filed Critical Basf Se
Publication of BR112017011759A2 publication Critical patent/BR112017011759A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P25/00—Preparation of compounds containing alloxazine or isoalloxazine nucleus, e.g. riboflavin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

a presente invenção se refere a um método para degradar dna em uma amostra obtida por fermentação microbiana ou biotransformação, compreendendo tratar a amostra com uma combinação de temperatura aumentada e ph baixo. ela também se refere a um método para liberar dna de uma célula microbiana compreendendo incubar a célula microbiana a uma temperatura de 45°c a 55°c por duas a dez horas. finalmente, a presente invenção provê um método para produzir um produto compreendendo uma etapa de liberar o dna de uma célula microbiana e degradar o dito dna.
BR112017011759A 2014-12-10 2015-12-09 métodos para produzir um produto de interesse com células microbianas, para degradar dna e para romper uma célula microbiana. BR112017011759A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14197230 2014-12-10
PCT/EP2015/079174 WO2016091980A1 (en) 2014-12-10 2015-12-09 Method of removing dna from biotechnological products

Publications (1)

Publication Number Publication Date
BR112017011759A2 true BR112017011759A2 (pt) 2018-02-20

Family

ID=52101098

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017011759A BR112017011759A2 (pt) 2014-12-10 2015-12-09 métodos para produzir um produto de interesse com células microbianas, para degradar dna e para romper uma célula microbiana.

Country Status (10)

Country Link
US (1) US10301588B2 (pt)
EP (1) EP3230435B1 (pt)
JP (1) JP2017536834A (pt)
KR (1) KR102079490B1 (pt)
CN (1) CN107002112B (pt)
BR (1) BR112017011759A2 (pt)
CA (1) CA2969046A1 (pt)
DK (1) DK3230435T3 (pt)
MY (1) MY176569A (pt)
WO (1) WO2016091980A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180084135A (ko) 2015-12-02 2018-07-24 바스프 에스이 감소된 clr2 활성을 갖는 사상 진균에서 단백질을 생산하는 방법
US10597429B2 (en) 2015-12-02 2020-03-24 Basf Se Method of producing proteins in filamentous fungi with decreased CLR1 activity
CN106566820B (zh) * 2016-09-20 2019-07-16 江南大学 α-淀粉酶的制备方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4165250A (en) 1975-08-29 1979-08-21 Merck & Co., Inc. Riboflavin purification
DE4037441A1 (de) 1990-11-24 1992-05-27 Basf Ag Verfahren zur erhoehung des riboflavin-gahaltes in spruehgetrockneten fermenteraustraegen bei riboflavin-fermentationen
DE69514549T2 (de) 1995-05-29 2000-08-24 Ni Skij I Vycislitel Nych Komp Verfahren zum erhalt von biomasse aus mikroorganismen mit geringem nukleinsäuregehalt
DE602004023101D1 (de) 2003-07-22 2009-10-22 Dsm Ip Assets Bv Verfahren zur aufreinigung von riboflavin
ITMI20032129A1 (it) 2003-11-05 2005-05-06 Acs Dobfar Spa Procedimento per la frammentazione del dna di cellule fungine, batteriche o di lieviti per la inattivazione di antibiotici residui in biomasse da fermentazione
DE10359660A1 (de) 2003-12-18 2005-07-28 Basf Ag Psod-Expressionseinheiten
EP1731617A1 (de) 2005-06-06 2006-12-13 DSM IP Assets B.V. Rückführung lysierter Biomasse als Nährmedium bei der fermentativen Herstellung von Riboflavin
EP2294217A4 (en) * 2008-05-23 2012-10-31 Life Technologies Corp METHODS FOR ELIMINATING NUCLEIC ACID CONTAMINATION FROM REAGENTS
EP2758514B2 (en) * 2011-09-22 2020-03-18 Danisco US Inc. Endogenous dnase activity to reduce dna content

Also Published As

Publication number Publication date
JP2017536834A (ja) 2017-12-14
KR102079490B1 (ko) 2020-02-20
CN107002112B (zh) 2021-11-02
EP3230435B1 (en) 2023-11-29
EP3230435A1 (en) 2017-10-18
WO2016091980A1 (en) 2016-06-16
US20170369835A1 (en) 2017-12-28
US10301588B2 (en) 2019-05-28
MY176569A (en) 2020-08-17
DK3230435T3 (da) 2024-02-12
CN107002112A (zh) 2017-08-01
CA2969046A1 (en) 2016-06-16
KR20170087511A (ko) 2017-07-28

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]