JP2022540849A - 抗凝固性多糖類の合成方法 - Google Patents

抗凝固性多糖類の合成方法 Download PDF

Info

Publication number
JP2022540849A
JP2022540849A JP2022501200A JP2022501200A JP2022540849A JP 2022540849 A JP2022540849 A JP 2022540849A JP 2022501200 A JP2022501200 A JP 2022501200A JP 2022501200 A JP2022501200 A JP 2022501200A JP 2022540849 A JP2022540849 A JP 2022540849A
Authority
JP
Japan
Prior art keywords
product
seq
sulfotransferase
glucosaminyl
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2022501200A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022540849A5 (https=
Inventor
ゲステイラ、タルシス、フェレイラ
ラジネス、ダニエル、エイチ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Optimvia LLC
Original Assignee
Optimvia LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Optimvia LLC filed Critical Optimvia LLC
Publication of JP2022540849A publication Critical patent/JP2022540849A/ja
Publication of JP2022540849A5 publication Critical patent/JP2022540849A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
    • A61K31/727—Heparin; Heparan
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0069—Chondroitin-4-sulfate, i.e. chondroitin sulfate A; Dermatan sulfate, i.e. chondroitin sulfate B or beta-heparin; Chondroitin-6-sulfate, i.e. chondroitin sulfate C; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0075—Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C08L5/10—Heparin; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/02—Sulfotransferases (2.8.2)
    • C12Y208/02008—[Heparan sulfate]-glucosamine N-sulfotransferase (2.8.2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/02—Sulfotransferases (2.8.2)
    • C12Y208/02023—[Heparan sulfate]-glucosamine 3-sulfotransferase 1 (2.8.2.23)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
    • C08J2305/10—Heparin; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03017—Heparosan-N-sulfate-glucuronate 5-epimerase (5.1.3.17)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Materials Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Dermatology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2022501200A 2019-07-09 2020-07-09 抗凝固性多糖類の合成方法 Pending JP2022540849A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962871980P 2019-07-09 2019-07-09
US62/871,980 2019-07-09
US202063033687P 2020-06-02 2020-06-02
US63/033,687 2020-06-02
PCT/US2020/041404 WO2021007429A1 (en) 2019-07-09 2020-07-09 Methods for synthesizing anticoagulant polysaccharides

Publications (2)

Publication Number Publication Date
JP2022540849A true JP2022540849A (ja) 2022-09-20
JP2022540849A5 JP2022540849A5 (https=) 2023-07-12

Family

ID=72046997

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022501200A Pending JP2022540849A (ja) 2019-07-09 2020-07-09 抗凝固性多糖類の合成方法

Country Status (9)

Country Link
US (3) US11542534B2 (https=)
EP (1) EP3997238A1 (https=)
JP (1) JP2022540849A (https=)
CN (1) CN114616340A (https=)
AU (1) AU2020310172A1 (https=)
BR (1) BR112022000255A8 (https=)
CA (1) CA3144968A1 (https=)
MX (1) MX2022000343A (https=)
WO (1) WO2021007429A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022015794A1 (en) 2020-07-14 2022-01-20 Optimvia, Llc Methods for synthesizing non-anticoagulant heparan sulfate
CN116284499B (zh) * 2022-07-28 2024-07-23 河北常山生化药业股份有限公司 一种羊源低分子肝素钠的制备方法
CN116120484B (zh) * 2022-09-01 2024-11-08 山东大学 一种抗凝血肝素寡糖苯联二聚体及其制备方法与应用
WO2025193841A1 (en) * 2024-03-13 2025-09-18 The Trustees Of Boston College Polysaccharides and methods of preparing same

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0833483A (ja) * 1994-07-22 1996-02-06 Seikagaku Kogyo Co Ltd ヘパラン硫酸６−ｏ−硫酸基転移酵素
WO1999022005A2 (en) * 1997-10-24 1999-05-06 Massachusetts Institute Of Technology Heparan sulfate d-glucosaminyl 3-o-sulfotransferases, an uses therefor
WO2004017910A2 (en) * 2002-08-23 2004-03-04 Neose Technologies, Inc. Total synthesis of heparin
JP2005507640A (ja) * 2001-03-28 2005-03-24 マサチューセッツ・インスティチュート・オブ・テクノロジー グルコサミニルの６−ｏ硫酸化方法及び６−ｏ硫酸化された多糖製剤
US20050255562A1 (en) * 2003-11-12 2005-11-17 Rosenberg Robert D Methods for synthesis of sulfated saccharides
WO2009014715A2 (en) * 2007-07-23 2009-01-29 The University Of North Carolina At Chapel Hill Enzymatic synthesis of sulfated polysaccharides without iduronic acid residues
WO2017115674A1 (ja) * 2015-12-28 2017-07-06 味の素株式会社 抗凝固活性を有するヘパラン硫酸の製造法

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3766167A (en) 1971-03-26 1973-10-16 Research Corp Orally active anticoagulant
DE2426586A1 (de) 1973-06-14 1975-01-09 Opocrin Srl Verfahren zur herstellung von glukuronil-glukosamin-glykan-sulfaten mit antilipaemischer wirkung
IT1083903B (it) 1977-08-09 1985-05-25 Lobo Srl Oligo-eteropolisaccaridi con attivita' eparinosimili,procedimento per il loro ottenimento e relative composizioni terapeutiche
US4500519A (en) 1978-11-06 1985-02-19 Choay S.A. Mucopolysaccharides having biological properties, preparation and method of use
US4692435A (en) 1978-11-06 1987-09-08 Choay, S.A. Mucopolysaccharide composition having a regulatory action on coagulation, medicament containing same and process of preparation
USRE35770E (en) 1978-11-06 1998-04-14 Choay, S.A. Oligosaccharides having anti-Xa activity and pharmaceutical compositions containing them
FR2440376A1 (fr) 1978-11-06 1980-05-30 Choay Sa Composition mucopolysaccharidique ayant une activite regulatrice de la coagulation, medicament la contenant et procede pour l'obtenir
FR2478646A2 (fr) 1980-03-20 1981-09-25 Choay Sa Composition mucopolysaccharidique ayant une activite regulatrice de la coagulation, medicament la contenant et procede pour l'obtenir
CA1136620A (en) 1979-01-08 1982-11-30 Ulf P.F. Lindahl Heparin fragments having selective anticoagulation activity
GB2051103A (en) 1979-04-11 1981-01-14 Choay Sa Heparin fractions
US4826827A (en) 1979-10-05 1989-05-02 Choay S.A. Short chained oligosaccharides having biological properties, a process for making the same and the use thereof as drugs
IL61201A (en) 1979-10-05 1984-09-30 Choay Sa Oligosaccharides having no more than 8 saccharide moieties,their obtention from heparin and pharmaceutical compositions containing them
US4281108A (en) 1980-01-28 1981-07-28 Hepar Industries, Inc. Process for obtaining low molecular weight heparins endowed with elevated pharmacological properties, and product so obtained
FR2482603A1 (fr) 1980-05-14 1981-11-20 Pharmindustrie Nouveaux esters d'heparine utilisables pour la preparation de medicaments, et procedes pour leur preparation
FR2482611B1 (fr) 1980-05-14 1986-03-07 Pharmindustrie Nouveaux polysaccharides sulfates, procedes pour leur preparation et leur utilisation comme medicaments
US4351938A (en) 1980-05-19 1982-09-28 Riker Laboratories, Inc. Anticoagulant substance
US4438261A (en) 1980-05-19 1984-03-20 Riker Laboratories, Inc. Anticoagulant substance
US4554101A (en) 1981-01-09 1985-11-19 New York Blood Center, Inc. Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity
FR2503714B1 (fr) 1981-04-10 1986-11-21 Choay Sa Procede d'obtention de mucopolysaccharides biologiquement actifs, de purete elevee, par depolymerisation de l'heparine
FR2504928A1 (fr) 1981-04-29 1982-11-05 Choay Sa Oligosaccharides a chaines courtes possedant des proprietes biologiques, leur preparation et leurs applications en tant que medicaments
US4396762A (en) 1981-08-24 1983-08-02 Massachusetts Institute Of Technology Heparinase derived anticoagulants
CA1195322A (en) 1982-07-19 1985-10-15 Eduardo Amaya Process for manufacturing low molecular weight heparins by depolymerization of normal heparin
US4533549A (en) 1983-01-04 1985-08-06 Lasker Sigmund E Antithrombotic agent
IT1195497B (it) 1983-03-08 1988-10-19 Opocrin Spa Procedimento per la preparazione di frazioni oligosaccaridiche dotate di proprieta' farmacologiche per degradazione chimica di eparina
US4686388A (en) 1985-03-12 1987-08-11 Pitney Bowes Inc. Integrated circuit substrate bias selection circuit
US5106734A (en) 1986-04-30 1992-04-21 Novo Nordisk A/S Process of using light absorption to control enzymatic depolymerization of heparin to produce low molecular weight heparin
DK196886D0 (da) 1986-04-30 1986-04-30 Novo Industri As Fremstilling af polysaccharider
DK196986D0 (da) 1986-04-30 1986-04-30 Novo Industri As Fremstilling af polysaccharider
IT1213384B (it) 1986-11-24 1989-12-20 Lab Derivati Organici Mediolan Processo per la preparazione controllata di gilcosaminoglicani a basso peso molecolare.
US4833155A (en) 1986-11-25 1989-05-23 Syntex (U.S.A.) Inc. 3-(ω-(3,5,-di-t-butyl-4-hydroxyphenyl)-alkanoyl)pyrroles, and anti-inflammatory uses thereof
ES2003197A6 (es) 1987-01-05 1988-10-16 Rovi Lab Farmaceut Sa Procedimiento de despolimerizacion de la heparina para la obtencion de una heparina de bajo peso molecular dotada de actividad antitrombotica
FR2614026B1 (fr) 1987-04-16 1992-04-17 Sanofi Sa Heparines de bas poids moleculaire, a structure reguliere, leur preparation et leurs applications biologiques
EP0337327A1 (en) 1988-04-09 1989-10-18 Bioiberica, S.A. Process for the preparation of new oligosaccharide fractions by controlled chemical depolimerization of heparin
IT1234508B (it) 1988-06-10 1992-05-19 Alfa Wassermann Spa Derivati eparinici e procedimento per la loro preparazione
US4981955A (en) 1988-06-28 1991-01-01 Lopez Lorenzo L Depolymerization method of heparin
IT1234826B (it) 1989-01-30 1992-05-29 Alfa Wassermann Spa Derivati eparinici e procedimento per la loro preparazione
DK0421508T3 (da) 1989-10-04 1994-04-11 Akzo Nobel Nv Sulfateret glycosaminoglycuronan med antithrombotisk aktivitet
US5378829A (en) 1990-04-23 1995-01-03 Akzo N.V. Sulfated glycosaminoglycanoid derivatives of the heparin and heparan sulfate type
FR2663639B1 (fr) 1990-06-26 1994-03-18 Rhone Poulenc Sante Melanges de polysaccharides de bas poids moleculaires procede de preparation et utilisation.
USRE38743E1 (en) 1990-06-26 2005-06-14 Aventis Pharma S.A. Mixtures of particular LMW heparinic polysaccharides for the prophylaxis/treatment of acute thrombotic events
US5821223A (en) 1990-09-14 1998-10-13 The United States Of America As Represented By The Department Of Health And Human Services Method of stimulating cell growth with a novel broad spectrum human lung fibroblast-derived mitogen
US5280016A (en) 1991-03-29 1994-01-18 Glycomed Incorporated Non-anticoagulant heparin derivatives
US5849721A (en) 1992-02-07 1998-12-15 Rhone-Poulenc Rorer S.A. Sulfated polysaccharides obtained from heparin, preparation process, pharmaceutical composition and use thereof
US5541095A (en) 1992-06-16 1996-07-30 University Of Massachusetts Medical Center Glycosaminoglycan specific sulfotransferases
FR2704861B1 (fr) 1993-05-07 1995-07-28 Sanofi Elf Fractions d'héparine purifiées, procédé d'obtention et compositions pharmaceutiques les contenant.
US5919673A (en) 1995-03-22 1999-07-06 The Scripps Research Institute One-pot enzymatic sulfation process using 3'-phosphoadenosine-5'-phosphosulfate and recycled phosphorylated adenosine intermediates
JP3672359B2 (ja) 1995-07-24 2005-07-20 生化学工業株式会社 ヘパラン硫酸 ２−ｏ−硫酸基転移酵素
US6861254B1 (en) 1997-10-24 2005-03-01 Massachusetts Institute Of Technology Heparan sulfate D-glucosaminyl 3-O-sulfotransferases, and uses therefor
US20060188966A1 (en) 1998-04-02 2006-08-24 Deangelis Paul L Natural, chimeric and hybrid glycosaminoglycan polymers and methods of making and using same
US7307159B2 (en) 2001-05-08 2007-12-11 The Board Of Regents Of The University Of Oklahoma Heparin/heparosan synthase from P. multocida and methods of making and using same
US8088604B2 (en) 1998-04-02 2012-01-03 The Board Of Regents Of The University Of Oklahoma Production of defined monodisperse heparosan polymers and unnatural polymers with polysaccharide synthases
JP2002518354A (ja) 1998-06-16 2002-06-25 ザ ボード オブ リージェンツ オブ ザ ユニヴァーシティー オブ オクラホマ 糖硫酸ペプチド、その合成方法および使用方法
US6255088B1 (en) 1999-05-11 2001-07-03 The Scripps Research Institute Enzymatic sulfation of biomolecules
DE10002005A1 (de) 2000-01-19 2001-08-09 Bosch Gmbh Robert Zerstäubungsdüse
CN1311305A (zh) * 2000-03-02 2001-09-05 上海博德基因开发有限公司 一种新的多肽——硫酸乙酰肝素3－0－磺基转移酶19和编码这种多肽的多核苷酸
ATE426805T1 (de) 2000-09-12 2009-04-15 Massachusetts Inst Technology Verfahren und produkte, die mit niedermolekularem heparin assoziiert sind
FR2814463B1 (fr) 2000-09-22 2002-11-15 Sanofi Synthelabo Nouveaux polysaccharides a activite antithrombotique comprenant au moins une liaison covalente avec la biotine ou un derive de la biotine
ITMI20010397A1 (it) 2001-02-27 2002-08-27 Giorgio Zoppetti Derivati altamente n,o-solfatati del polisaccaride k5 e loro preparazione
US8580290B2 (en) 2001-05-08 2013-11-12 The Board Of Regents Of The University Of Oklahoma Heparosan-based biomaterials and coatings and methods of production and use thereof
TWI281861B (en) 2002-01-29 2007-06-01 Sanofi Aventis A pharmaceutical composition comprising SANORG 34006 for treatment and secondary prophylaxis of venous thromboembolic events in patients with deep venous thrombosis
AU2003247808A1 (en) 2002-07-05 2004-01-23 Anders Malmstrom Purified and isolated heparan sulfate 3-o-sulfotransferase isoform 5 nucleic acids and polypeptides and therapeutic and screening methods using same
WO2004041197A2 (en) 2002-11-01 2004-05-21 Case Western Reserve University Methods of inhibiting glial scar formation
WO2004050673A2 (en) 2002-11-27 2004-06-17 Massachusetts Institute Of Technology Methods for synthesizing polysaccharides
WO2006023487A2 (en) * 2004-08-16 2006-03-02 Massachusetts Institute Of Technology Rapid two-step synthesis of anti-coagulants
WO2006083328A2 (en) 2004-09-15 2006-08-10 Massachusetts Institute Of Technology Biologically active surfaces and methods of their use
CA2594013A1 (en) 2005-01-12 2006-07-20 Massachusetts Institute Of Technology Methods and compositions related to modulating the extracellular stem cell environment
EP1885865A4 (en) 2005-05-12 2010-12-22 Univ North Carolina ENZYMATIC SYNTHESIS OF SULFATED POLYSACCHARIDES
DE602006011282D1 (de) 2005-07-05 2010-02-04 Huawei Tech Co Ltd Authentifizierungsverfahren für das ip multimedia subsystem
EP2447285A3 (en) 2006-05-25 2013-01-16 Momenta Pharmaceuticals, Inc. Low molecular weight heparin composition and uses thereof
ES2567079T3 (es) 2007-11-02 2016-04-19 Momenta Pharmaceuticals, Inc. Composiciones de polisacáridos que no son anticoagulantes
AU2008318343B2 (en) 2007-11-02 2013-07-18 Dilafor Ab Non-anticoagulant polysaccharide compositions
JP2011526925A (ja) 2008-07-01 2011-10-20 ザカロン ファーマシューティカルズ，インク． ヘパラン硫酸阻害剤
CA2777099C (en) 2009-07-31 2018-03-27 Reliable Biopharmaceutical Corporation Process for preparing fondaparinux sodium and intermediates useful in the synthesis thereof
CN101724086B (zh) 2009-11-25 2012-09-26 深圳海王药业有限公司 低聚凤梨参糖胺聚糖及其制备方法
WO2012088416A2 (en) * 2010-12-23 2012-06-28 The University Of North Carolina At Chapel Hill Chemoenzymatic synthesis of structurally homogeneous ultra-low molecular weight heparins
WO2012116048A1 (en) 2011-02-22 2012-08-30 Rensselaer Polytechnic Institute Single step heparosan n-deacetylation and depolymerization for making bioengineered heparin
US8815529B2 (en) 2011-05-23 2014-08-26 Research & Diagnostics Systems, Inc. Sulfotransferase assay
PL2831237T3 (pl) 2012-03-30 2018-06-29 The Board Of Regents Of The University Of Oklahoma Polimery heparosanu o wysokiej masie cząsteczkowej i sposoby ich wytwarzania i zastosowania
CN102660610A (zh) * 2012-05-31 2012-09-12 江南大学 一种酶法制备高活性低分子量肝素的方法
US20140349962A1 (en) 2013-05-24 2014-11-27 Massachusetts Institute Of Technology Rapid two-step synthesis of anti-coagulants
JP6670235B2 (ja) 2013-06-17 2020-03-18 ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒルＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｎｏｒｔｈ Ｃａｒｏｌｉｎａ Ａｔ Ｃｈａｐｅｌ Ｈｉｌｌ 可逆的ヘパリン分子、その製法及びその使用方法
HUE047658T2 (hu) 2014-08-22 2020-05-28 Cysbio Aps Biológiai eljárás szulfatált fenolvegyület elõállítására
EP3420095A1 (en) 2016-02-24 2019-01-02 Danmarks Tekniske Universitet Improved biological processes for the production of aryl sulfates
TWI769176B (zh) 2016-09-07 2022-07-01 瑞瑟勒綜合技術協會 生合成肝素
JP7088026B2 (ja) 2017-01-19 2022-06-21 味の素株式会社 ヘキスロン酸残基が異性化されたヘパロサン化合物の製造方法
CN116622006B (zh) 2017-03-10 2025-05-30 北卡罗来纳大学查珀尔希尔分校 短效的基于肝素的抗凝血剂化合物和方法
EP3679147B1 (en) 2017-09-05 2024-08-21 Ajinomoto Co., Inc. 2-o-sulfation enzyme mutant and 3-o-sulfation enzyme mutant, and method for using same
CN108220365A (zh) * 2018-01-16 2018-06-29 浙江海洋大学 一种提高肝素抗凝血活性的方法
JP2022523638A (ja) * 2019-01-15 2022-04-26 オプティムヴィア、エルエルシー 改変型硫酸アリール依存性酵素

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0833483A (ja) * 1994-07-22 1996-02-06 Seikagaku Kogyo Co Ltd ヘパラン硫酸６−ｏ−硫酸基転移酵素
WO1999022005A2 (en) * 1997-10-24 1999-05-06 Massachusetts Institute Of Technology Heparan sulfate d-glucosaminyl 3-o-sulfotransferases, an uses therefor
JP2005507640A (ja) * 2001-03-28 2005-03-24 マサチューセッツ・インスティチュート・オブ・テクノロジー グルコサミニルの６−ｏ硫酸化方法及び６−ｏ硫酸化された多糖製剤
WO2004017910A2 (en) * 2002-08-23 2004-03-04 Neose Technologies, Inc. Total synthesis of heparin
US20050255562A1 (en) * 2003-11-12 2005-11-17 Rosenberg Robert D Methods for synthesis of sulfated saccharides
WO2009014715A2 (en) * 2007-07-23 2009-01-29 The University Of North Carolina At Chapel Hill Enzymatic synthesis of sulfated polysaccharides without iduronic acid residues
WO2017115674A1 (ja) * 2015-12-28 2017-07-06 味の素株式会社 抗凝固活性を有するヘパラン硫酸の製造法

Also Published As

Publication number Publication date
CN114616340A (zh) 2022-06-10
US20230340552A1 (en) 2023-10-26
US20230013513A1 (en) 2023-01-19
BR112022000255A8 (pt) 2022-03-22
AU2020310172A1 (en) 2022-03-03
WO2021007429A1 (en) 2021-01-14
MX2022000343A (es) 2022-03-11
US11542534B2 (en) 2023-01-03
US11629364B2 (en) 2023-04-18
US20220010344A1 (en) 2022-01-13
BR112022000255A2 (pt) 2022-03-03
EP3997238A1 (en) 2022-05-18
CA3144968A1 (en) 2021-01-14

Similar Documents

Publication Publication Date Title
US11542534B2 (en) Methods for synthesizing anticoagulant polysaccharides
US11773382B2 (en) Engineered aryl sulfate-dependent enzymes
JP2022003136A (ja) グルコサミン残基の３−ｏ−硫酸化率が高いヘパラン硫酸
US11708593B2 (en) Engineered aryl sulfate-dependent enzymes
US20230151339A1 (en) Engineered aryl sulfate-dependent enzymes
KR20220032086A (ko) 항응고제 다당류의 합성 방법
EP4182452A1 (en) Methods for synthesizing non-anticoagulant heparan sulfate
US20240076634A1 (en) Engineered aryl sulfate-dependent enzymes
HK1261704B (en) Heparan sulfate having high 3-o-sulfation rate of glucosamine residues
HK1261704A1 (en) Heparan sulfate having high 3-o-sulfation rate of glucosamine residues

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230704

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20230704

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20240604

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20240826

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20241224