JP2022523638A - 改変型硫酸アリール依存性酵素 - Google Patents

改変型硫酸アリール依存性酵素 Download PDF

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JP2022523638A
JP2022523638A JP2021541244A JP2021541244A JP2022523638A JP 2022523638 A JP2022523638 A JP 2022523638A JP 2021541244 A JP2021541244 A JP 2021541244A JP 2021541244 A JP2021541244 A JP 2021541244A JP 2022523638 A JP2022523638 A JP 2022523638A
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seq
sulfotransferase
amino acid
enzyme
glucosaminyl
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JPWO2020150350A5 (https=
JP2022523638A5 (https=
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フェレイラ、タルシス、ゲステイラ
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Optimvia LLC
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Optimvia LLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/64—Preparation of S-glycosides, e.g. lincomycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/02—Sulfotransferases (2.8.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/02—Sulfotransferases (2.8.2)
    • C12Y208/02008—[Heparan sulfate]-glucosamine N-sulfotransferase (2.8.2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/02—Sulfotransferases (2.8.2)
    • C12Y208/02023—[Heparan sulfate]-glucosamine 3-sulfotransferase 1 (2.8.2.23)

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  • Chemical & Material Sciences (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
JP2021541244A 2019-01-15 2020-01-15 改変型硫酸アリール依存性酵素 Pending JP2022523638A (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201962792440P 2019-01-15 2019-01-15
US62/792,440 2019-01-15
US201962797466P 2019-01-28 2019-01-28
US62/797,466 2019-01-28
US201962808074P 2019-02-20 2019-02-20
US62/808,074 2019-02-20
US201962853261P 2019-05-28 2019-05-28
US62/853,261 2019-05-28
PCT/US2020/013677 WO2020150350A1 (en) 2019-01-15 2020-01-15 Engineered aryl sulfate-dependent enzymes

Publications (3)

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JP2022523638A true JP2022523638A (ja) 2022-04-26
JPWO2020150350A5 JPWO2020150350A5 (https=) 2023-01-20
JP2022523638A5 JP2022523638A5 (https=) 2023-01-20

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JP2021541244A Pending JP2022523638A (ja) 2019-01-15 2020-01-15 改変型硫酸アリール依存性酵素

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US (9) US11692180B2 (https=)
EP (1) EP3911737A1 (https=)
JP (1) JP2022523638A (https=)
KR (1) KR20210126019A (https=)
CN (1) CN113646427A (https=)
AU (1) AU2020209822A1 (https=)
BR (1) BR112021013890A2 (https=)
CA (1) CA3126805A1 (https=)
MX (1) MX2021008479A (https=)
WO (1) WO2020150350A1 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113646427A (zh) * 2019-01-15 2021-11-12 奥普蒂姆维亚有限公司 工程化芳基硫酸酯依赖性酶
WO2022015794A1 (en) 2020-07-14 2022-01-20 Optimvia, Llc Methods for synthesizing non-anticoagulant heparan sulfate
CA3144968A1 (en) * 2019-07-09 2021-01-14 Optimvia Llc Methods for synthesizing anticoagulant polysaccharides
CN114763570B (zh) * 2021-09-10 2024-02-27 江南大学 制备不同分子量肝素的磺酸化修饰体系构建及其应用
CN117467632B (zh) * 2022-07-27 2024-09-24 江南大学 一种肝素n-磺基转移酶突变体构建方法
CN115975040B (zh) * 2022-07-28 2026-02-27 重庆东渝中能实业有限公司 埃氏拟杆菌肝素酶i融合蛋白及其编码基因和制备方法
CN119662591B (zh) * 2025-01-20 2025-11-04 江南大学 一种肝素2-o-磺基转移酶高活性突变体的构建方法

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JP2005507640A (ja) * 2001-03-28 2005-03-24 マサチューセッツ・インスティチュート・オブ・テクノロジー グルコサミニルの６−ｏ硫酸化方法及び６−ｏ硫酸化された多糖製剤

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WO1999022005A2 (en) * 1997-10-24 1999-05-06 Massachusetts Institute Of Technology Heparan sulfate d-glucosaminyl 3-o-sulfotransferases, an uses therefor
JP2005507640A (ja) * 2001-03-28 2005-03-24 マサチューセッツ・インスティチュート・オブ・テクノロジー グルコサミニルの６−ｏ硫酸化方法及び６−ｏ硫酸化された多糖製剤

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US20220340888A9 (en) 2022-10-27
US11572549B2 (en) 2023-02-07
US11692180B2 (en) 2023-07-04
AU2020209822A1 (en) 2021-09-09
US11773382B2 (en) 2023-10-03
US20220042062A1 (en) 2022-02-10
US20210363503A1 (en) 2021-11-25
CA3126805A1 (en) 2020-07-23
US20240002819A1 (en) 2024-01-04
BR112021013890A2 (pt) 2021-09-21
US20230029317A1 (en) 2023-01-26
CN113646427A (zh) 2021-11-12
US11473068B2 (en) 2022-10-18
US20240093166A1 (en) 2024-03-21
US20230051957A1 (en) 2023-02-16
US11572550B2 (en) 2023-02-07
MX2021008479A (es) 2021-10-13
US20210363505A1 (en) 2021-11-25
EP3911737A1 (en) 2021-11-24
US11767518B2 (en) 2023-09-26
US20210363504A1 (en) 2021-11-25
KR20210126019A (ko) 2021-10-19
WO2020150350A1 (en) 2020-07-23
US20230092183A1 (en) 2023-03-23
US11708567B2 (en) 2023-07-25

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