CA3126805A1 - Engineered aryl sulfate-dependent enzymes - Google Patents

Engineered aryl sulfate-dependent enzymes Download PDF

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Publication number
CA3126805A1
CA3126805A1 CA3126805A CA3126805A CA3126805A1 CA 3126805 A1 CA3126805 A1 CA 3126805A1 CA 3126805 A CA3126805 A CA 3126805A CA 3126805 A CA3126805 A CA 3126805A CA 3126805 A1 CA3126805 A1 CA 3126805A1
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CA
Canada
Prior art keywords
seq
sulfotransferase
amino acid
glucosaminyl
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3126805A
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English (en)
French (fr)
Inventor
Tarsis Gesteira Ferreira
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Optimvia LLC
Original Assignee
Optimvia LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Optimvia LLC filed Critical Optimvia LLC
Publication of CA3126805A1 publication Critical patent/CA3126805A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/64—Preparation of S-glycosides, e.g. lincomycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/02—Sulfotransferases (2.8.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/02—Sulfotransferases (2.8.2)
    • C12Y208/02008—[Heparan sulfate]-glucosamine N-sulfotransferase (2.8.2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/02—Sulfotransferases (2.8.2)
    • C12Y208/02023—[Heparan sulfate]-glucosamine 3-sulfotransferase 1 (2.8.2.23)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
CA3126805A 2019-01-15 2020-01-15 Engineered aryl sulfate-dependent enzymes Pending CA3126805A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201962792440P 2019-01-15 2019-01-15
US62/792,440 2019-01-15
US201962797466P 2019-01-28 2019-01-28
US62/797,466 2019-01-28
US201962808074P 2019-02-20 2019-02-20
US62/808,074 2019-02-20
US201962853261P 2019-05-28 2019-05-28
US62/853,261 2019-05-28
PCT/US2020/013677 WO2020150350A1 (en) 2019-01-15 2020-01-15 Engineered aryl sulfate-dependent enzymes

Publications (1)

Publication Number Publication Date
CA3126805A1 true CA3126805A1 (en) 2020-07-23

Family

ID=69529057

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3126805A Pending CA3126805A1 (en) 2019-01-15 2020-01-15 Engineered aryl sulfate-dependent enzymes

Country Status (10)

Country Link
US (9) US11692180B2 (https=)
EP (1) EP3911737A1 (https=)
JP (1) JP2022523638A (https=)
KR (1) KR20210126019A (https=)
CN (1) CN113646427A (https=)
AU (1) AU2020209822A1 (https=)
BR (1) BR112021013890A2 (https=)
CA (1) CA3126805A1 (https=)
MX (1) MX2021008479A (https=)
WO (1) WO2020150350A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
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CN113646427A (zh) * 2019-01-15 2021-11-12 奥普蒂姆维亚有限公司 工程化芳基硫酸酯依赖性酶
WO2022015794A1 (en) 2020-07-14 2022-01-20 Optimvia, Llc Methods for synthesizing non-anticoagulant heparan sulfate
CA3144968A1 (en) * 2019-07-09 2021-01-14 Optimvia Llc Methods for synthesizing anticoagulant polysaccharides
CN114763570B (zh) * 2021-09-10 2024-02-27 江南大学 制备不同分子量肝素的磺酸化修饰体系构建及其应用
CN117467632B (zh) * 2022-07-27 2024-09-24 江南大学 一种肝素n-磺基转移酶突变体构建方法
CN115975040B (zh) * 2022-07-28 2026-02-27 重庆东渝中能实业有限公司 埃氏拟杆菌肝素酶i融合蛋白及其编码基因和制备方法
CN119662591B (zh) * 2025-01-20 2025-11-04 江南大学 一种肝素2-o-磺基转移酶高活性突变体的构建方法

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JP3818676B2 (ja) 1994-07-22 2006-09-06 生化学工業株式会社 ヘパラン硫酸６−ｏ−硫酸基転移酵素
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Publication number Publication date
US20220340888A9 (en) 2022-10-27
US11572549B2 (en) 2023-02-07
US11692180B2 (en) 2023-07-04
AU2020209822A1 (en) 2021-09-09
US11773382B2 (en) 2023-10-03
US20220042062A1 (en) 2022-02-10
US20210363503A1 (en) 2021-11-25
US20240002819A1 (en) 2024-01-04
BR112021013890A2 (pt) 2021-09-21
US20230029317A1 (en) 2023-01-26
CN113646427A (zh) 2021-11-12
US11473068B2 (en) 2022-10-18
US20240093166A1 (en) 2024-03-21
US20230051957A1 (en) 2023-02-16
US11572550B2 (en) 2023-02-07
MX2021008479A (es) 2021-10-13
US20210363505A1 (en) 2021-11-25
JP2022523638A (ja) 2022-04-26
EP3911737A1 (en) 2021-11-24
US11767518B2 (en) 2023-09-26
US20210363504A1 (en) 2021-11-25
KR20210126019A (ko) 2021-10-19
WO2020150350A1 (en) 2020-07-23
US20230092183A1 (en) 2023-03-23
US11708567B2 (en) 2023-07-25

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