JP2021501577A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2021501577A5 JP2021501577A5 JP2020524114A JP2020524114A JP2021501577A5 JP 2021501577 A5 JP2021501577 A5 JP 2021501577A5 JP 2020524114 A JP2020524114 A JP 2020524114A JP 2020524114 A JP2020524114 A JP 2020524114A JP 2021501577 A5 JP2021501577 A5 JP 2021501577A5
- Authority
- JP
- Japan
- Prior art keywords
- binding
- item
- nucleic acid
- kit
- polymer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 175
- 239000000126 substance Substances 0.000 claims description 115
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 101
- 229920001184 polypeptide Polymers 0.000 claims description 83
- 229920002521 macromolecule Polymers 0.000 claims description 60
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 claims description 52
- 239000007787 solid Substances 0.000 claims description 51
- 239000011230 binding agent Substances 0.000 claims description 47
- 150000007523 nucleic acids Chemical class 0.000 claims description 45
- 108020004707 nucleic acids Proteins 0.000 claims description 43
- 102000039446 nucleic acids Human genes 0.000 claims description 43
- 102000004169 proteins and genes Human genes 0.000 claims description 37
- 108090000623 proteins and genes Proteins 0.000 claims description 37
- 125000001433 C-terminal amino-acid group Chemical group 0.000 claims description 33
- 239000012634 fragment Substances 0.000 claims description 28
- 238000004458 analytical method Methods 0.000 claims description 23
- 239000012491 analyte Substances 0.000 claims description 22
- 239000003153 chemical reaction reagent Substances 0.000 claims description 19
- 125000000539 amino acid group Chemical group 0.000 claims description 7
- 238000009396 hybridization Methods 0.000 claims description 5
- 230000004481 post-translational protein modification Effects 0.000 claims description 5
- 230000002000 scavenging effect Effects 0.000 claims description 3
- 150000001720 carbohydrates Chemical class 0.000 claims description 2
- 150000002632 lipids Chemical class 0.000 claims description 2
- 230000000295 complement effect Effects 0.000 claims 2
- 229920000642 polymer Polymers 0.000 description 286
- 238000000034 method Methods 0.000 description 181
- 239000011324 bead Substances 0.000 description 52
- 238000012163 sequencing technique Methods 0.000 description 24
- 230000037452 priming Effects 0.000 description 11
- 125000000729 N-terminal amino-acid group Chemical group 0.000 description 10
- 150000004676 glycans Chemical class 0.000 description 10
- 229920001282 polysaccharide Polymers 0.000 description 10
- 239000005017 polysaccharide Substances 0.000 description 10
- 125000006850 spacer group Chemical group 0.000 description 10
- 108020004414 DNA Proteins 0.000 description 9
- 108010085220 Multiprotein Complexes Proteins 0.000 description 8
- 102000007474 Multiprotein Complexes Human genes 0.000 description 8
- 239000000758 substrate Substances 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 7
- 239000011521 glass Substances 0.000 description 7
- 238000003203 nucleic acid sequencing method Methods 0.000 description 7
- 108091023037 Aptamer Proteins 0.000 description 6
- 239000000020 Nitrocellulose Substances 0.000 description 6
- 108010043958 Peptoids Proteins 0.000 description 6
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 6
- 239000012528 membrane Substances 0.000 description 6
- 229920001220 nitrocellulos Polymers 0.000 description 6
- 229910052710 silicon Inorganic materials 0.000 description 6
- 239000010703 silicon Substances 0.000 description 6
- 102000004400 Aminopeptidases Human genes 0.000 description 5
- 108090000915 Aminopeptidases Proteins 0.000 description 5
- 108091005804 Peptidases Proteins 0.000 description 5
- 102000035195 Peptidases Human genes 0.000 description 5
- 239000004365 Protease Substances 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 230000001404 mediated effect Effects 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 150000003384 small molecules Chemical class 0.000 description 5
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 4
- 229920000936 Agarose Polymers 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 4
- 239000004952 Polyamide Substances 0.000 description 4
- 239000004793 Polystyrene Substances 0.000 description 4
- 239000013043 chemical agent Substances 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 230000005298 paramagnetic effect Effects 0.000 description 4
- 229920002647 polyamide Polymers 0.000 description 4
- 229920002223 polystyrene Polymers 0.000 description 4
- 239000011148 porous material Substances 0.000 description 4
- 238000000018 DNA microarray Methods 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 229930193936 anticarin Natural products 0.000 description 3
- 238000003491 array Methods 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 238000003384 imaging method Methods 0.000 description 3
- 235000019689 luncheon sausage Nutrition 0.000 description 3
- 239000004005 microsphere Substances 0.000 description 3
- 239000002105 nanoparticle Substances 0.000 description 3
- 229920001778 nylon Polymers 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 238000012175 pyrosequencing Methods 0.000 description 3
- 239000004065 semiconductor Substances 0.000 description 3
- 101800001415 Bri23 peptide Proteins 0.000 description 2
- 101800000655 C-terminal peptide Proteins 0.000 description 2
- 102400000107 C-terminal peptide Human genes 0.000 description 2
- 108010016626 Dipeptides Proteins 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 108010006035 Metalloproteases Proteins 0.000 description 2
- 102000005741 Metalloproteases Human genes 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 125000003275 alpha amino acid group Chemical group 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000021615 conjugation Effects 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000003278 mimic effect Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- LOTKRQAVGJMPNV-UHFFFAOYSA-N 1-fluoro-2,4-dinitrobenzene Chemical compound [O-][N+](=O)C1=CC=C(F)C([N+]([O-])=O)=C1 LOTKRQAVGJMPNV-UHFFFAOYSA-N 0.000 description 1
- OVOJUAKDTOOXRF-UHFFFAOYSA-N 2,4-dinitrobenzenesulfonic acid Chemical compound OS(=O)(=O)C1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O OVOJUAKDTOOXRF-UHFFFAOYSA-N 0.000 description 1
- GWGBNENHEGYJSN-UHFFFAOYSA-N 2,4-dinitrobenzenesulfonic acid;hydrate Chemical compound O.OS(=O)(=O)C1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O GWGBNENHEGYJSN-UHFFFAOYSA-N 0.000 description 1
- DLZKEQQWXODGGZ-KCJUWKMLSA-N 2-[[(2r)-2-[[(2s)-2-amino-3-(4-hydroxyphenyl)propanoyl]amino]propanoyl]amino]acetic acid Chemical compound OC(=O)CNC(=O)[C@@H](C)NC(=O)[C@@H](N)CC1=CC=C(O)C=C1 DLZKEQQWXODGGZ-KCJUWKMLSA-N 0.000 description 1
- BAILAVMHWMOTOK-UHFFFAOYSA-N 2-fluoro-1-nitro-5-sulfonylcyclohexa-1,3-diene Chemical compound S(=O)(=O)=C1CC(=C(C=C1)F)[N+](=O)[O-] BAILAVMHWMOTOK-UHFFFAOYSA-N 0.000 description 1
- 102100032488 Acylamino-acid-releasing enzyme Human genes 0.000 description 1
- 108010061216 Acylaminoacyl-peptidase Proteins 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 108091008102 DNA aptamers Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108010079855 Peptide Aptamers Proteins 0.000 description 1
- INOFNEWQYCGESC-UHFFFAOYSA-N acetic acid;chromen-2-one Chemical compound CC(O)=O.C1=CC=C2OC(=O)C=CC2=C1 INOFNEWQYCGESC-UHFFFAOYSA-N 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 125000003172 aldehyde group Chemical group 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 239000007806 chemical reaction intermediate Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 101150038575 clpS gene Proteins 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 150000002540 isothiocyanates Chemical class 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 108091008104 nucleic acid aptamers Proteins 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000816 peptidomimetic Substances 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762579844P | 2017-10-31 | 2017-10-31 | |
| US62/579,844 | 2017-10-31 | ||
| US201762582312P | 2017-11-06 | 2017-11-06 | |
| US62/582,312 | 2017-11-06 | ||
| US201762583448P | 2017-11-08 | 2017-11-08 | |
| US62/583,448 | 2017-11-08 | ||
| PCT/US2018/058565 WO2019089836A1 (en) | 2017-10-31 | 2018-10-31 | Kits for analysis using nucleic acid encoding and/or label |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021501577A JP2021501577A (ja) | 2021-01-21 |
| JP2021501577A5 true JP2021501577A5 (enExample) | 2021-12-09 |
| JP7390027B2 JP7390027B2 (ja) | 2023-12-01 |
Family
ID=66333623
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020524114A Active JP7390027B2 (ja) | 2017-10-31 | 2018-10-31 | 核酸エンコーディングおよび／または標識を使用する解析のためのキット |
Country Status (10)
| Country | Link |
|---|---|
| US (6) | US11513126B2 (enExample) |
| EP (1) | EP3704249B1 (enExample) |
| JP (1) | JP7390027B2 (enExample) |
| KR (1) | KR102556494B1 (enExample) |
| CN (2) | CN119716077A (enExample) |
| AU (1) | AU2018358057B2 (enExample) |
| CA (1) | CA3081441C (enExample) |
| MX (1) | MX2020004559A (enExample) |
| SG (1) | SG11202003924YA (enExample) |
| WO (1) | WO2019089836A1 (enExample) |
Families Citing this family (85)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE112012002570B4 (de) | 2011-06-23 | 2023-11-23 | Board Of Regents, The University Of Texas System | Identifizieren von Peptiden auf der Einzelmolekülebene |
| US11435358B2 (en) | 2011-06-23 | 2022-09-06 | Board Of Regents, The University Of Texas System | Single molecule peptide sequencing |
| WO2016069124A1 (en) | 2014-09-15 | 2016-05-06 | Board Of Regents, The University Of Texas System | Improved single molecule peptide sequencing |
| EP4299803A3 (en) | 2016-05-02 | 2024-03-27 | Encodia, Inc. | Macromolecule analysis employing nucleic acid encoding |
| DK3548652T3 (da) | 2016-12-01 | 2024-07-08 | Nautilus Subsidiary Inc | Fremgangsmåder til proteinanalyse |
| AU2018358057B2 (en) | 2017-10-31 | 2023-03-02 | Encodia, Inc. | Kits for analysis using nucleic acid encoding and/or label |
| SG11202003923PA (en) | 2017-10-31 | 2020-05-28 | Encodia Inc | Methods and kits using nucleic acid encoding and/or label |
| IL326759A (en) | 2017-12-29 | 2026-04-01 | Nautilus Subsidiary Inc | Decoding approaches for protein identification |
| CN112236528A (zh) * | 2018-04-04 | 2021-01-15 | 诺迪勒思生物科技公司 | 产生纳米阵列和微阵列的方法 |
| US11302421B2 (en) * | 2018-04-13 | 2022-04-12 | The Hong Kong Polytechnic University | Data storage using peptides |
| CA3110800A1 (en) | 2018-07-12 | 2020-01-16 | Board Of Regents, The University Of Texas System | Molecular neighborhood detection by oligonucleotides |
| CN108866046A (zh) * | 2018-07-25 | 2018-11-23 | 辽宁润基生物科技有限公司 | 一种分离、纯化样品中外泌体和/或游离核酸的方法 |
| CA3117476A1 (en) | 2018-10-05 | 2020-04-09 | Board Of Regents, The University Of Texas System | Solid-phase n-terminal peptide capture and release |
| US10836798B2 (en) * | 2018-11-08 | 2020-11-17 | Government Of The United States Of America, As Represented By The Secretary Of Commerce | Amino acid-specific binder and selectively identifying an amino acid |
| TWI863941B (zh) | 2018-11-15 | 2024-12-01 | 美商寬騰矽公司 | 用於蛋白質定序之方法及組合物 |
| US11427814B2 (en) | 2019-03-26 | 2022-08-30 | Encodia, Inc. | Modified cleavases, uses thereof and related kits |
| CA3137716A1 (en) | 2019-04-23 | 2020-10-29 | Encodia, Inc. | Methods for spatial analysis of proteins and related kits |
| CN114072499B (zh) | 2019-04-30 | 2024-08-06 | Encodia公司 | 用于制备分析物的方法和相关试剂盒 |
| US20220227889A1 (en) * | 2019-04-30 | 2022-07-21 | Encodia, Inc. | Methods and reagents for cleavage of the n-terminal amino acid from a polypeptide |
| EP3973299A4 (en) * | 2019-05-20 | 2023-04-19 | Encodia, Inc. | METHODS AND RELATED KITS FOR SPATIAL ANALYSIS |
| US20240042446A1 (en) * | 2019-10-18 | 2024-02-08 | Encodia, Inc. | Automated treatment of macromolecules for analysis and related apparatus |
| US20210148921A1 (en) * | 2019-10-28 | 2021-05-20 | Quantum-Si Incorporated | Methods of preparing an enriched sample for polypeptide sequencing |
| AU2020376809A1 (en) * | 2019-10-28 | 2022-06-02 | Quantum-Si Incorporated | Methods, kits and devices of preparing samples for multiplex polypeptide sequencing |
| JP2023501932A (ja) * | 2019-10-28 | 2023-01-20 | クアンタム－エスアイ インコーポレイテッド | 単一ペプチドの配列決定及び再構築の方法 |
| WO2021141924A1 (en) | 2020-01-07 | 2021-07-15 | Encodia, Inc. | Methods for stable complex formation and related kits |
| WO2021141922A1 (en) * | 2020-01-07 | 2021-07-15 | Encodia, Inc. | Methods for information transfer and related kits |
| EP4073263A4 (en) | 2020-01-07 | 2023-11-08 | Encodia, Inc. | METHOD FOR STABLE COMPLEX FORMATION AND CORRESPONDING KITS |
| EP3969611B1 (en) * | 2020-02-03 | 2025-12-10 | Illumina, Inc. | Biotin-streptavidin cleavage composition and library fragment cleavage |
| JP7445966B2 (ja) * | 2020-03-24 | 2024-03-08 | 国立研究開発法人産業技術総合研究所 | 糖鎖を解析する方法 |
| WO2021236983A2 (en) | 2020-05-20 | 2021-11-25 | Quantum-Si Incorporated | Methods and compositions for protein sequencing |
| AU2021288692A1 (en) | 2020-06-11 | 2023-02-02 | Nautilus Subsidiary, Inc. | Methods and systems for computational decoding of biological, chemical, and physical entities |
| CN116685725A (zh) * | 2020-08-19 | 2023-09-01 | Encodia 公司 | 顺序编码方法和相关试剂盒 |
| WO2022072560A1 (en) * | 2020-09-30 | 2022-04-07 | Encodia, Inc. | Polypeptide terminal binders and uses thereof |
| WO2022094148A1 (en) | 2020-10-30 | 2022-05-05 | Encodia, Inc. | Conjugation reagents and methods using 1,2-cyclohexanediones |
| KR20230118570A (ko) | 2020-11-11 | 2023-08-11 | 노틸러스 서브시디어리, 인크. | 강화된 결합 및 검출 특성을 갖는 친화성 시약 |
| EP4271997A4 (en) * | 2020-12-31 | 2024-12-04 | Encodia, Inc. | METALLOENZYMES FOR THE BIOMOLECULAR RECOGNITION OF N-TERMINAL MODIFIED PEPTIDES |
| US12092642B2 (en) | 2021-01-20 | 2024-09-17 | Nautilus Subsidiary, Inc. | Systems and methods for biomolecule quantitation |
| EP4281775B1 (en) | 2021-01-21 | 2026-04-29 | Nautilus Subsidiary, Inc. | Systems and methods for biomolecule preparation |
| CN112899152B (zh) * | 2021-02-02 | 2023-11-17 | 中国科学院苏州纳米技术与纳米仿生研究所 | 核酸快速扩增和检测的微流控芯片、检测方法及系统 |
| WO2022173690A1 (en) * | 2021-02-09 | 2022-08-18 | University Of Washington | Methods for protein identification through combinatorial barcoding |
| IL305336B2 (en) | 2021-03-11 | 2025-09-01 | Nautilus Subsidiary Inc | Systems and methods for preserving biomolecules |
| CN113129996B (zh) * | 2021-03-22 | 2022-04-12 | 复旦大学 | 一种基于分子动力学模拟的核酸适配体优化设计方法 |
| WO2022200793A1 (en) * | 2021-03-25 | 2022-09-29 | The University Of Birmingham | Analyte capture |
| WO2022217112A1 (en) * | 2021-04-09 | 2022-10-13 | Ultima Genomics, Inc. | Systems and methods for spatial screening of analytes |
| CN113281507B (zh) * | 2021-05-23 | 2022-08-16 | 吉林大学 | 一种金黄色葡萄球菌快速检测方法及试剂盒 |
| WO2022271983A1 (en) | 2021-06-24 | 2022-12-29 | Nautilus Biotechnology, Inc. | Methods and systems for assay refinement |
| CA3227872A1 (en) | 2021-09-09 | 2023-03-16 | James Henry JOLY | Characterization and localization of protein modifications |
| JP2024537689A (ja) * | 2021-09-22 | 2024-10-16 | ノーティラス・サブシディアリー・インコーポレイテッド | ポリペプチド相互作用を決定するための方法及びシステム |
| WO2023081728A1 (en) | 2021-11-03 | 2023-05-11 | Nautilus Biotechnology, Inc. | Systems and methods for surface structuring |
| US12474346B2 (en) | 2021-11-05 | 2025-11-18 | Encodia, Inc. | Methods and kits for multicycle encoding assay |
| EP4430206A1 (en) | 2021-11-10 | 2024-09-18 | Encodia, Inc. | Methods for barcoding macromolecules in individual cells |
| US12612656B2 (en) | 2021-11-30 | 2026-04-28 | Nautilus Subsidiary, Inc. | Particle-based isolation of proteins and other analytes |
| CN113881753B (zh) * | 2021-12-08 | 2022-02-18 | 南京邮电大学 | 一种非诊断目的的基因定位方法 |
| US20250093339A1 (en) * | 2021-12-15 | 2025-03-20 | BioLegend, Inc. | Compositions and methods for enhanced sample multiplexing |
| EP4206674B1 (en) | 2021-12-28 | 2025-10-22 | Encodia, Inc. | High-throughput serotyping and antibody profiling assays |
| US12092578B2 (en) | 2022-03-29 | 2024-09-17 | Nautilus Subsidiary, Inc. | Integrated arrays for single-analyte processes |
| EP4508435A4 (en) * | 2022-04-15 | 2026-04-15 | Encodia Inc | HIGH-SPEED PEPTIDE IDENTIFICATION USING CONJUGATED BINDERS AND KINETIC CODING |
| WO2023212490A1 (en) | 2022-04-25 | 2023-11-02 | Nautilus Subsidiary, Inc. | Systems and methods for assessing and improving the quality of multiplex molecular assays |
| WO2023241720A1 (zh) * | 2022-06-17 | 2023-12-21 | 上海高探生物科技有限公司 | 样本处理系统及其制备方法 |
| EP4544077A1 (en) | 2022-06-21 | 2025-04-30 | Nautilus Subsidiary, Inc. | Method for detecting analytes at sites of optically non-resolvable distances |
| CA3261351A1 (en) * | 2022-07-12 | 2024-01-18 | Abrus Bio, Inc. | DETERMINATION OF PROTEIN INFORMATION BY RECODED AMINO ACID POLYMERS INTO DNA POLYMERS |
| WO2024030919A1 (en) | 2022-08-02 | 2024-02-08 | Glyphic Biotechnologies, Inc. | Protein sequencing via coupling of polymerizable molecules |
| WO2024040236A2 (en) | 2022-08-19 | 2024-02-22 | Abrus Bio, Inc. | Determination of protein information by recoding amino acid polymers into dna polymers |
| WO2024058967A1 (en) | 2022-09-13 | 2024-03-21 | Nautilus Subsidiary, Inc. | Systems and methods of validating new affinity reagents |
| WO2024059655A1 (en) | 2022-09-15 | 2024-03-21 | Nautilus Subsidiary, Inc. | Characterizing accessibility of macromolecule structures |
| WO2024073599A1 (en) | 2022-09-29 | 2024-04-04 | Nautilus Subsidiary, Inc. | Preparation of array surfaces for single-analyte processes |
| US20240192202A1 (en) | 2022-12-09 | 2024-06-13 | Nautilus Subsidiary, Inc. | Fluidic media for single-analtye arrays |
| WO2024130000A1 (en) | 2022-12-15 | 2024-06-20 | Nautilus Subsidiary, Inc. | Inhibition of photon phenomena on single molecule arrays |
| US20240301469A1 (en) | 2023-02-24 | 2024-09-12 | Nautilus Subsidiary, Inc. | Modifying, separating and detecting proteoforms |
| CN115976655A (zh) * | 2023-03-20 | 2023-04-18 | 深圳市小分子新药创新中心有限公司 | On-DNA酰肼化合物的合成方法、DNA编码化合物库 |
| EP4689173A1 (en) | 2023-03-24 | 2026-02-11 | Nautilus Subsidiary, Inc. | Improved transfer of nanoparticles to array surfaces |
| EP4720641A1 (en) | 2023-05-24 | 2026-04-08 | Nautilus Subsidiary, Inc. | Systems and methods for machine vision |
| EP4732011A1 (en) | 2023-06-21 | 2026-04-29 | Nautilus Subsidiary, Inc. | Compositions and methods for improving affinity reagent avidity |
| CN117019248B (zh) * | 2023-08-17 | 2025-05-02 | 杭州跃真生物科技有限公司 | 一种微孔芯片与单细胞分析方法 |
| US20250066841A1 (en) | 2023-08-24 | 2025-02-27 | Nautilus Subsidiary, Inc. | Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions |
| WO2025080862A1 (en) * | 2023-10-10 | 2025-04-17 | Fluid Discovery | Quantitative partitioned amplification |
| GB202317028D0 (en) * | 2023-11-06 | 2023-12-20 | Oxford Nanopore Tech Ltd | Method |
| WO2025128588A1 (en) | 2023-12-11 | 2025-06-19 | Nautilus Subsidiary, Inc. | Methods and compositions of particle-based arrays |
| US20250205706A1 (en) | 2023-12-20 | 2025-06-26 | Nautilus Subsidiary, Inc. | Fluidic devices for single-analyte assays |
| WO2025199236A1 (en) | 2024-03-22 | 2025-09-25 | Nautilus Subsidiary, Inc. | Plasmonic particle systems for single-analyte assays |
| US20250305034A1 (en) | 2024-04-01 | 2025-10-02 | Nautilus Subsidiary, Inc. | Nanostructures for modulation of analyte conformation |
| GB2642439A (en) * | 2024-07-05 | 2026-01-14 | Gitlife Biotech Ltd | Methods and compositions |
| US20260092914A1 (en) | 2024-09-27 | 2026-04-02 | Nautilus Subsidiary, Inc. | Single analyte characterization under equilibrium binding conditions |
| CN119851760B (zh) * | 2025-01-14 | 2025-09-30 | 大连理工大学 | 一种基于三进制dna编码库的核酸检测方法 |
| CN120078882A (zh) * | 2025-05-07 | 2025-06-03 | 中国人民解放军军事科学院军事医学研究院 | 一种多糖-蛋白缀合物、其纳米颗粒以及疫苗组合物 |
Family Cites Families (161)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4863870A (en) | 1988-03-10 | 1989-09-05 | Bio-Affinity Systems, Inc. | Method of thioacylation peptide sequencing with alcoholysis of thiazolinones |
| US5484735A (en) | 1989-08-23 | 1996-01-16 | Northwestern University | Immunoassay of glycosylated proteins employing antibody directed to reductively glycosylated N-terminal amino acids |
| US5049507A (en) | 1989-12-21 | 1991-09-17 | Applied Biosystems, Inc. | Method of C-terminal peptide sequencing |
| DK0604552T3 (da) | 1991-09-18 | 1997-08-04 | Affymax Tech Nv | Fremgangsmåde til syntese af forskellige samlinger af oligomerer |
| US5573905A (en) | 1992-03-30 | 1996-11-12 | The Scripps Research Institute | Encoded combinatorial chemical libraries |
| SE9400088D0 (sv) | 1994-01-14 | 1994-01-14 | Kabi Pharmacia Ab | Bacterial receptor structures |
| US5834252A (en) | 1995-04-18 | 1998-11-10 | Glaxo Group Limited | End-complementary polymerase reaction |
| GB9421819D0 (en) | 1994-10-29 | 1994-12-14 | Cancer Res Campaign Tech | Sequence analysis of saccharide material |
| CA2176414A1 (en) | 1995-05-18 | 1996-11-19 | S. David Kimball | Acyl guanidine and amidine prodrugs |
| CA2225553A1 (en) | 1995-08-03 | 1997-02-20 | Rijksuniversiteit Te Leiden | Cell derived antigen presenting vesicles |
| JP3124507B2 (ja) | 1996-05-24 | 2001-01-15 | セイコーインスツルメンツ株式会社 | タンパク質あるいはペプチドのカルボキシ末端からのアミノ酸配列を決定する方法 |
| US5900481A (en) | 1996-11-06 | 1999-05-04 | Sequenom, Inc. | Bead linkers for immobilizing nucleic acids to solid supports |
| DK0971946T3 (da) * | 1997-01-21 | 2006-10-30 | Gen Hospital Corp | Selektion af proteiner ved anvendelse af RNA-protein-fusioner |
| US6306588B1 (en) | 1997-02-07 | 2001-10-23 | Invitrogen Corporation | Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof |
| WO2000034527A2 (en) | 1998-12-11 | 2000-06-15 | The Regents Of The University Of California | Targeted molecular bar codes |
| US6875851B1 (en) | 1999-03-05 | 2005-04-05 | University Of Georgia Research Foundation, Inc. | Prolyl tripeptidyl peptidases nucleic acid of Porphyromonas gingivalis |
| DE60029572T2 (de) * | 1999-03-23 | 2007-07-26 | Biovation Ltd. | Isolierung und analyse von proteinen |
| WO2000071755A2 (en) | 1999-05-25 | 2000-11-30 | Praelux Incorporated | Method for sequency and characterizing polymeric biomolecules using aptamers and a method for producing aptamers |
| IL147037A0 (en) * | 1999-07-27 | 2002-08-14 | Phylos Inc | Peptide acceptor ligation methods |
| GB9927320D0 (en) | 1999-11-18 | 2000-01-12 | Chiron Spa | Exosome separation |
| US7306904B2 (en) | 2000-02-18 | 2007-12-11 | Olink Ab | Methods and kits for proximity probing |
| US6511809B2 (en) | 2000-06-13 | 2003-01-28 | E. I. Du Pont De Nemours And Company | Method for the detection of an analyte by means of a nucleic acid reporter |
| CA2314398A1 (en) | 2000-08-10 | 2002-02-10 | Edward Shipwash | Microarrays and microsystems for amino acid analysis and protein sequencing |
| ATE538380T1 (de) | 2001-01-23 | 2012-01-15 | Harvard College | Nukleinsäure-programmierbare protein-arrays |
| US20030087232A1 (en) | 2001-01-25 | 2003-05-08 | Fred Christians | Methods for screening polypeptides |
| US20030118585A1 (en) | 2001-10-17 | 2003-06-26 | Agy Therapeutics | Use of protein biomolecular targets in the treatment and visualization of brain tumors |
| EP1401850A1 (en) | 2001-06-20 | 2004-03-31 | Nuevolution A/S | Nucleoside derivatives for library preparation |
| AU2002330671B2 (en) * | 2001-07-06 | 2008-04-03 | Centre National De La Recherche Scientifique (Cnrs) | Method for obtaining cells with new properties |
| WO2003029462A1 (en) | 2001-09-27 | 2003-04-10 | Pieris Proteolab Ag | Muteins of human neutrophil gelatinase-associated lipocalin and related proteins |
| US7169894B2 (en) | 2001-11-14 | 2007-01-30 | Mirari Biosciences, Inc. | Methods and compositions for reverse translation |
| WO2003044231A1 (en) | 2001-11-23 | 2003-05-30 | Simon Fredriksson | Method and kit for proximity probing with multivalent proximity probes |
| DE10242016A1 (de) | 2002-09-11 | 2004-03-25 | Esplora GmbH c/o TU Darmstadt Institut für Biochemie | Verfahren zur Identifizierung BHS-spezifischer Proteine und Fragmente davon |
| BR0314558A (pt) | 2002-10-10 | 2005-08-09 | Diversa Corp | Proteases, ácidos nucléicos codificando as mesmas e processos para preparar e usar as mesmas |
| US20090246879A1 (en) * | 2002-12-20 | 2009-10-01 | Callida Genomics | Materials and Methods Relating to Nano-Tags and Nano-Brands |
| WO2005015239A2 (en) | 2003-08-07 | 2005-02-17 | Cornell Research Foundation, Inc. | Method for n-terminal labeling of proteins |
| US7198900B2 (en) | 2003-08-29 | 2007-04-03 | Applera Corporation | Multiplex detection compositions, methods, and kits |
| WO2005026387A1 (en) | 2003-09-18 | 2005-03-24 | Nuevolution A/S | A method for obtaining structural information concerning an encoded molecule and method for selecting compounds |
| EP1561755A2 (en) | 2003-10-16 | 2005-08-10 | Shimadzu Corporation | Method for derivatizing protein or peptide to sulfonic acid derivative |
| RU2470077C2 (ru) | 2003-12-17 | 2012-12-20 | ГЛЭКСОСМИТКЛАЙН ЭлЭлСи | Биологически активное соединение, содержащее кодирующий олигонуклеотид (варианты), способ его синтеза, библиотека соединений (варианты), способ ее синтеза и способ поиска соединения, связывающегося с биологической мишенью (варианты) |
| GB0404187D0 (en) | 2004-02-25 | 2004-03-31 | Biotransformations Ltd | Binding agents |
| WO2005090566A2 (en) | 2004-03-22 | 2005-09-29 | Nuevolution A/S | Ligational encoding using building block oligonucleotides |
| US20050260653A1 (en) | 2004-04-14 | 2005-11-24 | Joshua Labaer | Nucleic-acid programmable protein arrays |
| US7393665B2 (en) | 2005-02-10 | 2008-07-01 | Population Genetics Technologies Ltd | Methods and compositions for tagging and identifying polynucleotides |
| US20060199279A1 (en) * | 2005-03-07 | 2006-09-07 | Viorica Lopez-Avila | Methods for identifying post-translationally modified polypeptides |
| US7229769B2 (en) | 2005-03-25 | 2007-06-12 | Illumina, Inc. | Compositions and methods for detecting protease activity |
| CA2541986A1 (en) | 2005-04-21 | 2006-10-21 | Co2 Solution Inc. | Carbonic anhydrase having increased stability under high temperatue conditions |
| US7883848B2 (en) | 2005-07-08 | 2011-02-08 | Olink Ab | Regulation analysis by cis reactivity, RACR |
| US20070020640A1 (en) | 2005-07-21 | 2007-01-25 | Mccloskey Megan L | Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis |
| US7611834B2 (en) | 2005-09-30 | 2009-11-03 | Sandia Corporation | Methods and devices for protein assays |
| LT2336315T (lt) * | 2005-12-01 | 2017-11-10 | Nuevolution A/S | Fermentiniai kodavimo būdai, skirti efektyviai didelių bibliotekų sintezei |
| US20070218503A1 (en) | 2006-02-13 | 2007-09-20 | Mitra Robi D | Methods of polypeptide identification, and compositions therefor |
| DE102006015001A1 (de) | 2006-03-31 | 2007-10-11 | NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen | Verfahren zum Nachweis und/oder zur Anreicherung von Analytproteinen und/oder Analytpeptiden aus einer komplexen Proteinmischung |
| WO2007127848A1 (en) | 2006-04-26 | 2007-11-08 | University Of Louisville Research Foundation, Inc | Isolation of membrane vesicles from biological fluids and methods of using same |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| WO2008085912A1 (en) | 2007-01-05 | 2008-07-17 | Massachusetts Institute Of Technology | Compositions of and methods of using sulfatases from flavobacterium heparinum |
| US8568994B2 (en) | 2007-08-03 | 2013-10-29 | Dkfz Deutsches Krebsforschungszentrum, Stiftung Des Offentlichen Rechts | Method for prenatal diagnosis |
| EP2210109A1 (en) * | 2007-10-19 | 2010-07-28 | Pronota NV | Preparation of samples for proteome analysis |
| WO2010065531A1 (en) | 2008-12-01 | 2010-06-10 | Robi David Mitra | Single molecule protein screening |
| CA2745197A1 (en) | 2008-12-01 | 2010-06-10 | Research Triangle Institute | Concurrent identification of multitudes of polypeptides |
| WO2010127186A1 (en) | 2009-04-30 | 2010-11-04 | Prognosys Biosciences, Inc. | Nucleic acid constructs and methods of use |
| US9566335B1 (en) | 2009-09-25 | 2017-02-14 | The Governing Council Of The University Of Toronto | Protein sequencing method and reagents |
| US9175340B2 (en) | 2009-11-04 | 2015-11-03 | President And Fellows Of Harvard College | Reactivity-dependent and interaction-dependent PCR |
| US20110245101A1 (en) | 2010-04-05 | 2011-10-06 | Prognosys Biosciences, Inc. | Co-localization affinity assays |
| AU2011237729B2 (en) | 2010-04-05 | 2014-04-03 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| WO2011127219A1 (en) | 2010-04-06 | 2011-10-13 | Caris Life Sciences Luxembourg Holdings | Circulating biomarkers for disease |
| US20130059741A1 (en) | 2010-05-13 | 2013-03-07 | Illumina, Inc. | Binding assays for markers |
| WO2012041633A1 (en) | 2010-09-27 | 2012-04-05 | Vipergen | A method for making an enriched library |
| EP2638057B1 (en) | 2010-11-10 | 2019-03-06 | Exosome Diagnostics, Inc. | Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom |
| GB201101621D0 (en) * | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
| EP2675819B1 (en) | 2011-02-18 | 2020-04-08 | Bio-Rad Laboratories, Inc. | Compositions and methods for molecular labeling |
| EP2694709B1 (en) | 2011-04-08 | 2016-09-14 | Prognosys Biosciences, Inc. | Peptide constructs and assay systems |
| AU2012242847B2 (en) | 2011-04-15 | 2017-01-19 | The Johns Hopkins University | Safe sequencing system |
| ES2744589T3 (es) | 2011-05-11 | 2020-02-25 | Exosome Diagnostics Inc | Extracción de ácido nucleico de materiales biológicos heterogéneos |
| WO2016069124A1 (en) | 2014-09-15 | 2016-05-06 | Board Of Regents, The University Of Texas System | Improved single molecule peptide sequencing |
| DE112012002570B4 (de) | 2011-06-23 | 2023-11-23 | Board Of Regents, The University Of Texas System | Identifizieren von Peptiden auf der Einzelmolekülebene |
| WO2013028788A1 (en) | 2011-08-22 | 2013-02-28 | Exosome Diagnostics, Inc. | Urine biomarkers |
| US20130052665A1 (en) | 2011-08-25 | 2013-02-28 | Bruce Xuefeng Ling | Methods for diagnosis of systemic juvenile idiopathic arthritis |
| US9335292B2 (en) * | 2011-10-13 | 2016-05-10 | Auburn University | Electrochemical proximity assay |
| US10174361B2 (en) | 2011-11-10 | 2019-01-08 | Exosome Diagnostics, Inc. | Cerebrospinal fluid assay |
| WO2013101896A1 (en) | 2011-12-30 | 2013-07-04 | Quest Diagnostics Investments Incorporated | Nucleic acid analysis using emulsion pcr |
| US20150087526A1 (en) | 2012-01-24 | 2015-03-26 | The Regents Of The University Of Colorado, A Body Corporate | Peptide identification and sequencing by single-molecule detection of peptides undergoing degradation |
| HUE053360T2 (hu) | 2012-02-17 | 2021-06-28 | Hutchinson Fred Cancer Res | Kompozíciók és eljárások mutációk pontos azonosítására |
| EP2825675B1 (en) | 2012-03-13 | 2017-12-27 | Patel, Abhijit Ajit | Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing |
| ES2828661T3 (es) | 2012-03-20 | 2021-05-27 | Univ Washington Through Its Center For Commercialization | Métodos para reducir la tasa de error de la secuenciación de ADN masiva en paralelo mediante el uso de la secuenciación de secuencia consenso bicatenaria |
| US9005888B2 (en) | 2012-06-14 | 2015-04-14 | System Biosciences, Llc | Methods for microvesicle isolation and selective removal |
| US9267127B2 (en) | 2012-06-21 | 2016-02-23 | President And Fellows Of Harvard College | Evolution of bond-forming enzymes |
| NL2009191C2 (en) | 2012-07-16 | 2014-01-20 | Univ Delft Tech | Single molecule protein sequencing. |
| US20140378322A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| JP6367196B2 (ja) | 2012-08-14 | 2018-08-01 | テンエックス・ジェノミクス・インコーポレイテッド | マイクロカプセル組成物および方法 |
| US9557336B2 (en) | 2012-09-07 | 2017-01-31 | University Of Rochester | Methods and compositions for site-specific labeling of peptides and proteins |
| CN105025878A (zh) | 2012-10-03 | 2015-11-04 | 外来体诊断公司 | 微泡在医学疾病和病况的诊断、预后和治疗中的用途 |
| KR20150090072A (ko) | 2012-10-23 | 2015-08-05 | 카리스 라이프 사이언스 스위스 홀딩스 게엠베하 | 압타머 및 이의 용도 |
| US9605309B2 (en) | 2012-11-09 | 2017-03-28 | Genia Technologies, Inc. | Nucleic acid sequencing using tags |
| EP2925337B1 (en) | 2012-11-21 | 2019-07-03 | The Trustees of Columbia University in the City of New York | Mutant ngal proteins and uses thereof |
| US9435810B2 (en) | 2013-03-15 | 2016-09-06 | Washington University | Molecules and methods for iterative polypeptide analysis and processing |
| EP2971071B1 (en) | 2013-03-15 | 2018-02-28 | Illumina, Inc. | Enzyme-linked nucleotides |
| EP3611262B1 (en) | 2013-03-15 | 2020-11-11 | Lineage Biosciences, Inc. | Methods of sequencing the immune repertoire |
| WO2014145123A2 (en) | 2013-03-15 | 2014-09-18 | Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State Unversity | Peptide array quality control |
| US10053725B2 (en) | 2013-04-23 | 2018-08-21 | President And Fellows Of Harvard College | In situ interaction determination |
| WO2014189768A1 (en) | 2013-05-19 | 2014-11-27 | The Board Of Trustees Of The Leland | Devices and methods for display of encoded peptides, polypeptides, and proteins on dna |
| EP3004385B1 (en) | 2013-05-24 | 2018-11-28 | Quantapore Inc. | Nanopore-based nucleic acid analysis with mixed fret detection |
| US10065988B2 (en) | 2013-05-31 | 2018-09-04 | Stefano Menegatti | Peptoid affinity ligands |
| WO2014201273A1 (en) | 2013-06-12 | 2014-12-18 | The Broad Institute, Inc. | High-throughput rna-seq |
| DK3013983T3 (da) | 2013-06-25 | 2023-03-06 | Prognosys Biosciences Inc | Spatialt kodede biologiske assays ved brug af en mikrofluidisk anordning |
| US10876177B2 (en) | 2013-07-10 | 2020-12-29 | President And Fellows Of Harvard College | Compositions and methods relating to nucleic acid-protein complexes |
| WO2015031694A2 (en) | 2013-08-28 | 2015-03-05 | Caris Science, Inc. | Oligonucleotide probes and uses thereof |
| US10466248B2 (en) | 2013-09-23 | 2019-11-05 | The Trustees Of Columbia University In The City Of New York | High-throughput single molecule protein identification |
| US10288608B2 (en) | 2013-11-08 | 2019-05-14 | Prognosys Biosciences, Inc. | Polynucleotide conjugates and methods for analyte detection |
| WO2015073538A2 (en) | 2013-11-12 | 2015-05-21 | Echogen, Inc. | Interaction with metalloenzymes |
| US9834814B2 (en) | 2013-11-22 | 2017-12-05 | Agilent Technologies, Inc. | Spatial molecular barcoding of in situ nucleic acids |
| WO2015131153A1 (en) | 2014-02-27 | 2015-09-03 | Board Of Regents, The University Of Texas System | Methods and compositions for isolating exosomes |
| US10858698B2 (en) | 2014-03-25 | 2020-12-08 | President And Fellows Of Harvard College | Barcoded protein array for multiplex single-molecule interaction profiling |
| LT3134536T (lt) | 2014-04-21 | 2020-05-11 | President And Fellows Of Harvard College | Sistemos ir būdai, skirti nukleorūgščių brūkšniniam kodavimui |
| CN106459160B (zh) | 2014-04-24 | 2022-03-29 | 南洋理工大学 | Asx特异性蛋白质连接酶 |
| JP6593710B2 (ja) | 2014-05-02 | 2019-10-23 | Ｉｄａｃセラノスティクス株式会社 | 単一細胞由来核酸の解析方法 |
| US20170074855A1 (en) * | 2014-05-15 | 2017-03-16 | Two Pore Guys, Inc. | Scaffold Data Storage and Target Detection in a Sample Using a Nanopore |
| EP4574974A3 (en) | 2014-06-26 | 2025-10-08 | 10x Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| WO2015200869A1 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| WO2016004068A1 (en) | 2014-06-30 | 2016-01-07 | Jae-Won Shin | Hydrogel compositions comprising encapsulated cells and methods of use thereof |
| US10465183B2 (en) | 2014-07-09 | 2019-11-05 | Exosome Diagnostics, Inc. | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| US20160060687A1 (en) * | 2014-07-18 | 2016-03-03 | Cdi Laboratories, Inc. | Methods and compositions to identify, quantify, and characterize target analytes and binding moieties |
| EP4219710A3 (en) | 2014-08-01 | 2023-08-16 | Dovetail Genomics, LLC | Tagging nucleic acids for sequence assembly |
| JP6808617B2 (ja) | 2014-10-17 | 2021-01-06 | イルミナ ケンブリッジ リミテッド | 連続性を維持した転位 |
| US9771412B2 (en) | 2014-10-31 | 2017-09-26 | Wisconsin Alumni Research Foundation | Engineered intein for improved production of protein-intein fusions |
| EP3218477A4 (en) * | 2014-11-10 | 2018-07-18 | The Board of Trustees of The Leland Stanford Junior University | Compositions and methods for expressing polypeptides on the surface of cells |
| BR112017017025B1 (pt) | 2015-02-10 | 2024-01-02 | Illumina, Inc | Métodos e composições para analizar componentes celulares |
| CN107208158B (zh) * | 2015-02-27 | 2022-01-28 | 贝克顿迪金森公司 | 空间上可寻址的分子条形编码 |
| WO2016145416A2 (en) | 2015-03-11 | 2016-09-15 | The Broad Institute, Inc. | Proteomic analysis with nucleic acid identifiers |
| WO2016164530A1 (en) | 2015-04-07 | 2016-10-13 | University Of Maryland, College Park | Compositions and methods for high throughput protein sequencing |
| AU2016276750A1 (en) | 2015-06-09 | 2018-01-18 | The Board Of Regents Of The University Of Texas System | Diagnostic test for early stage cancer |
| CN108449941B (zh) | 2015-09-22 | 2022-03-11 | 豪夫迈·罗氏有限公司 | OmpG变体 |
| EP3766987B1 (en) | 2015-09-24 | 2023-08-02 | F. Hoffmann-La Roche AG | Alpha-hemolysin variants |
| EP3397764A4 (en) * | 2015-12-30 | 2019-05-22 | Bio-Rad Laboratories, Inc. | DIGITAL PROTEIN QUANTIFICATION |
| WO2017125565A1 (en) | 2016-01-21 | 2017-07-27 | Genia Technologies, Inc. | Nanopore sequencing complexes |
| EP3417078B1 (en) | 2016-02-17 | 2021-05-05 | President and Fellows of Harvard College | Molecular programming tools |
| EP4299803A3 (en) | 2016-05-02 | 2024-03-27 | Encodia, Inc. | Macromolecule analysis employing nucleic acid encoding |
| WO2017205719A1 (en) | 2016-05-27 | 2017-11-30 | President And Fellows Of Harvard College | Conditional primer extension for single-molecule detection |
| WO2018005720A1 (en) | 2016-06-30 | 2018-01-04 | President And Fellows Of Harvard College | Method of determining the molecular binding between libraries of molecules |
| EP3488018B1 (en) | 2016-07-22 | 2026-02-18 | President and Fellows of Harvard College | Methods for protein identification |
| DK3548652T3 (da) | 2016-12-01 | 2024-07-08 | Nautilus Subsidiary Inc | Fremgangsmåder til proteinanalyse |
| EP4310183B1 (en) | 2017-01-30 | 2025-07-09 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| EP4324931A3 (en) | 2017-02-02 | 2024-05-29 | New York Genome Center, Inc. | Methods and compositions for identifying or quantifying targets in a biological sample |
| AU2018358057B2 (en) | 2017-10-31 | 2023-03-02 | Encodia, Inc. | Kits for analysis using nucleic acid encoding and/or label |
| WO2019089846A1 (en) | 2017-10-31 | 2019-05-09 | Encodia, Inc. | Methods and compositions for polypeptide analysis |
| SG11202003923PA (en) | 2017-10-31 | 2020-05-28 | Encodia Inc | Methods and kits using nucleic acid encoding and/or label |
| IL326759A (en) | 2017-12-29 | 2026-04-01 | Nautilus Subsidiary Inc | Decoding approaches for protein identification |
| US20190219578A1 (en) | 2018-01-18 | 2019-07-18 | Nanosomix, Inc. | Dectection of exosomes and exosomal biomarkers for the diagnosis and prognosis of diseases and disorders |
| US12270741B2 (en) | 2018-04-03 | 2025-04-08 | Tymora Analytical Operations, Llc | Methods for affinity-based non-antibody capture and purification of extracellular vesicles |
| WO2020006409A1 (en) | 2018-06-28 | 2020-01-02 | Trustees Of Boston University | Systems and methods for control of gene expression |
| TWI863941B (zh) | 2018-11-15 | 2024-12-01 | 美商寬騰矽公司 | 用於蛋白質定序之方法及組合物 |
| CN113474651B (zh) | 2019-01-08 | 2024-05-17 | 麻省理工学院 | 单分子蛋白质和肽测序 |
| US11427814B2 (en) | 2019-03-26 | 2022-08-30 | Encodia, Inc. | Modified cleavases, uses thereof and related kits |
| AU2020247918B2 (en) | 2019-03-26 | 2022-06-30 | Encodia, Inc. | Modified cleavases, uses thereof and related kits |
| CN114072499B (zh) | 2019-04-30 | 2024-08-06 | Encodia公司 | 用于制备分析物的方法和相关试剂盒 |
| US20220227889A1 (en) | 2019-04-30 | 2022-07-21 | Encodia, Inc. | Methods and reagents for cleavage of the n-terminal amino acid from a polypeptide |
| WO2020252404A1 (en) | 2019-06-12 | 2020-12-17 | Obsidian Therapeutics, Inc. | Ca2 compositions and methods for tunable regulation |
| US11834664B2 (en) | 2019-09-13 | 2023-12-05 | Google Llc | Methods and compositions for protein and peptide sequencing |
| WO2022072560A1 (en) | 2020-09-30 | 2022-04-07 | Encodia, Inc. | Polypeptide terminal binders and uses thereof |
| WO2022094148A1 (en) | 2020-10-30 | 2022-05-05 | Encodia, Inc. | Conjugation reagents and methods using 1,2-cyclohexanediones |
| EP4271997A4 (en) | 2020-12-31 | 2024-12-04 | Encodia, Inc. | METALLOENZYMES FOR THE BIOMOLECULAR RECOGNITION OF N-TERMINAL MODIFIED PEPTIDES |
| EP4430206A1 (en) | 2021-11-10 | 2024-09-18 | Encodia, Inc. | Methods for barcoding macromolecules in individual cells |
-
2018
- 2018-10-31 AU AU2018358057A patent/AU2018358057B2/en active Active
- 2018-10-31 CA CA3081441A patent/CA3081441C/en active Active
- 2018-10-31 SG SG11202003924YA patent/SG11202003924YA/en unknown
- 2018-10-31 CN CN202411678798.5A patent/CN119716077A/zh active Pending
- 2018-10-31 EP EP18874783.6A patent/EP3704249B1/en active Active
- 2018-10-31 CN CN201880084553.6A patent/CN112272703B/zh active Active
- 2018-10-31 KR KR1020207015546A patent/KR102556494B1/ko active Active
- 2018-10-31 US US16/760,028 patent/US11513126B2/en active Active
- 2018-10-31 MX MX2020004559A patent/MX2020004559A/es unknown
- 2018-10-31 JP JP2020524114A patent/JP7390027B2/ja active Active
- 2018-10-31 WO PCT/US2018/058565 patent/WO2019089836A1/en not_active Ceased
-
2022
- 2022-08-11 US US17/819,263 patent/US12130291B2/en active Active
-
2023
- 2023-02-08 US US18/166,461 patent/US11782062B2/en active Active
-
2024
- 2024-07-05 US US18/764,943 patent/US12467928B2/en active Active
- 2024-07-29 US US18/787,946 patent/US12292446B2/en active Active
- 2024-10-22 US US18/923,452 patent/US20250060371A1/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP7097627B2 (ja) | 核酸エンコーディングを使用した巨大分子解析 | |
| US20240003892A1 (en) | Heterogeneous single cell profiling using molecular barcoding | |
| JP2019523635A5 (enExample) | ||
| US11634709B2 (en) | Methods for preparing analytes and related kits | |
| JP2021501577A (ja) | 核酸エンコーディングおよび／または標識を使用する解析のためのキット | |
| JP2021501576A5 (enExample) | ||
| CN105264088A (zh) | 提高鉴定细胞中的多个表位的动态范围 | |
| US20240309041A1 (en) | Methods for targeted protein quantification by bar-coding affinity reagent with unique dna sequences | |
| US20210396762A1 (en) | Methods for peptide analysis employing multi-component detection agent and related kits | |
| CN103703143A (zh) | 鉴定细胞中的多个表位的方法 | |
| JP2017525390A (ja) | 組換えタンパク質調製物中の残留宿主細胞タンパク質の検出 | |
| CN113624953A (zh) | 用于发现和分析治疗剂的平台 | |
| CA3137716A1 (en) | Methods for spatial analysis of proteins and related kits | |
| JP2022533226A (ja) | 空間分析のための方法および関連キット | |
| CN120092180A (zh) | 通过可聚合分子偶联进行蛋白质测序 | |
| CN114127281B (zh) | 邻近相互作用分析 | |
| WO2001084146A2 (en) | A sensitive, multiplexed mass-spectrometry-based biosensor based on immuno-pcr | |
| US20240044908A1 (en) | Methods for protein identification through combinatorial barcoding | |
| US12416100B2 (en) | Devices and methods for display of encoded peptides, polypeptides, and proteins on DNA | |
| AU2021329812B2 (en) | Sequential encoding methods and related kits | |
| US20080096767A1 (en) | Method For Expanding The Dynamic Detection Range In Microarrays | |
| US20250163406A1 (en) | Methods for generating nucleic acid encoded protein libraries and uses thereof | |
| US20240042446A1 (en) | Automated treatment of macromolecules for analysis and related apparatus | |
| CN119592667A (zh) | 用于分析生物样品的标志物、试剂盒和方法 | |
| CN120752535A (zh) | 肽测序仪 |