JP2020507312A5 - - Google Patents
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- JP2020507312A5 JP2020507312A5 JP2019539756A JP2019539756A JP2020507312A5 JP 2020507312 A5 JP2020507312 A5 JP 2020507312A5 JP 2019539756 A JP2019539756 A JP 2019539756A JP 2019539756 A JP2019539756 A JP 2019539756A JP 2020507312 A5 JP2020507312 A5 JP 2020507312A5
- Authority
- JP
- Japan
- Prior art keywords
- dna
- crispr
- ctag
- digested
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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- 108091033409 CRISPR Proteins 0.000 claims 42
- 108020004414 DNA Proteins 0.000 claims 34
- 238000000034 method Methods 0.000 claims 28
- 238000010354 CRISPR gene editing Methods 0.000 claims 22
- 238000003780 insertion Methods 0.000 claims 14
- 230000037431 insertion Effects 0.000 claims 14
- 230000000415 inactivating effect Effects 0.000 claims 6
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 5
- 102100025570 Cancer/testis antigen 1 Human genes 0.000 claims 4
- 101000856237 Homo sapiens Cancer/testis antigen 1 Proteins 0.000 claims 4
- 238000010367 cloning Methods 0.000 claims 3
- 238000011534 incubation Methods 0.000 claims 3
- 108020004707 nucleic acids Proteins 0.000 claims 3
- 102000039446 nucleic acids Human genes 0.000 claims 3
- 150000007523 nucleic acids Chemical class 0.000 claims 3
- 238000006467 substitution reaction Methods 0.000 claims 3
- 102000053602 DNA Human genes 0.000 claims 2
- 108060002716 Exonuclease Proteins 0.000 claims 2
- 108020005004 Guide RNA Proteins 0.000 claims 2
- 238000006243 chemical reaction Methods 0.000 claims 2
- 102000013165 exonuclease Human genes 0.000 claims 2
- 238000000746 purification Methods 0.000 claims 2
- 238000000926 separation method Methods 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- 102000003960 Ligases Human genes 0.000 claims 1
- 108090000364 Ligases Proteins 0.000 claims 1
- 230000029087 digestion Effects 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 125000006850 spacer group Chemical group 0.000 claims 1
- 230000008685 targeting Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762457493P | 2017-02-10 | 2017-02-10 | |
| US62/457,493 | 2017-02-10 | ||
| PCT/US2018/017573 WO2018148511A1 (en) | 2017-02-10 | 2018-02-09 | A modular universal plasmid design strategy for the assembly and editing of multiple dna constructs for multiple hosts |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2020507312A JP2020507312A (ja) | 2020-03-12 |
| JP2020507312A5 true JP2020507312A5 (cg-RX-API-DMAC7.html) | 2021-03-25 |
Family
ID=63107838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019539756A Withdrawn JP2020507312A (ja) | 2017-02-10 | 2018-02-09 | 複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US11098305B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP3580337A4 (cg-RX-API-DMAC7.html) |
| JP (1) | JP2020507312A (cg-RX-API-DMAC7.html) |
| KR (1) | KR20190116282A (cg-RX-API-DMAC7.html) |
| CN (1) | CN110312797A (cg-RX-API-DMAC7.html) |
| CA (1) | CA3049989A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2018148511A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190116282A (ko) | 2017-02-10 | 2019-10-14 | 지머젠 인코포레이티드 | 복수의 숙주를 위한 다중 dna 구조체의 조립 및 편집을 위한 모듈식 범용 플라스미드 디자인 전략 |
| JP2021533773A (ja) * | 2018-08-15 | 2021-12-09 | ザイマージェン インコーポレイテッド | ハイスループット代謝操作におけるＣＲＩＳＰＲｉの適用 |
| CN113795588B (zh) | 2019-04-04 | 2025-02-25 | 瑞泽恩制药公司 | 用于在靶向性载体中无瘢痕引入靶向修饰的方法 |
| CN114555803B (zh) * | 2019-10-14 | 2024-11-29 | 延世大学校产学协力团 | 新的前间区序列邻近基序序列以及利用其修饰细胞基因组中靶核酸的方法 |
| KR102178813B1 (ko) | 2019-10-18 | 2020-11-13 | 주식회사 다우진유전자연구소 | Dna 구조체를 적용한 간이 키트 |
| WO2021098709A1 (zh) * | 2019-11-18 | 2021-05-27 | 中国科学院遗传与发育生物学研究所 | 衍生自黄杆菌的基因编辑系统 |
| WO2021108324A1 (en) * | 2019-11-27 | 2021-06-03 | Technical University Of Denmark | Constructs, compositions and methods thereof having improved genome editing efficiency and specificity |
| US12018316B2 (en) | 2020-03-03 | 2024-06-25 | Telesis Bio Inc. | Methods for assembling nucleic acids |
| CN113373130B (zh) * | 2021-05-31 | 2023-12-22 | 复旦大学 | Cas12蛋白、含有Cas12蛋白的基因编辑系统及应用 |
| GB202117455D0 (en) * | 2021-12-02 | 2022-01-19 | Academisch Ziekenhuis Leiden | Method of editing nucleic acid |
| US20250223620A1 (en) * | 2022-04-08 | 2025-07-10 | Glaxosmithkline Intellectual Property Development Limited | Novel processes for the production of polynucleotides including oligonucleotides |
| CN116284436A (zh) * | 2022-11-25 | 2023-06-23 | 广州美格生物科技有限公司 | 嵌合体TsCas12a蛋白及其制备方法和应用 |
| CN119432813A (zh) * | 2023-12-29 | 2025-02-14 | 武汉艾迪晶生物科技有限公司 | Cas蛋白、组合蛋白、核酸分子、核糖核蛋白复合物、重组载体、转基因细胞及其应用 |
| WO2025165780A1 (en) * | 2024-02-01 | 2025-08-07 | The Board Of Trustees Of The Leland Stanford Junior University | Combinatorial gene editing and screening |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE246702T1 (de) | 1993-04-12 | 2003-08-15 | Univ Northwestern | Verfahren zur darstellung von oligonukleotiden |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| WO2000026381A2 (en) | 1998-10-30 | 2000-05-11 | Cornell Research Foundation, Inc. | High fidelity thermostable ligase and uses thereof |
| JP2012529287A (ja) * | 2009-06-11 | 2012-11-22 | トゥールゲン インコーポレイション | 部位−特異的ヌクレアーゼを用いた標的ゲノムの再配列 |
| GB2481425A (en) * | 2010-06-23 | 2011-12-28 | Iti Scotland Ltd | Method and device for assembling polynucleic acid sequences |
| WO2013176772A1 (en) | 2012-05-25 | 2013-11-28 | The Regents Of The University Of California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| ES2786193T3 (es) | 2012-12-12 | 2020-10-09 | Broad Inst Inc | Modificación por tecnología genética y optimización de sistemas, métodos y composiciones enzimáticas mejorados para la manipulación de secuencias |
| EP2840140B2 (en) | 2012-12-12 | 2023-02-22 | The Broad Institute, Inc. | Crispr-Cas based method for mutation of prokaryotic cells |
| US20140310830A1 (en) | 2012-12-12 | 2014-10-16 | Feng Zhang | CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes |
| WO2014093655A2 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| PL2771468T3 (pl) | 2012-12-12 | 2015-07-31 | Broad Inst Inc | Inżynieria systemów, metod i zoptymalizowanych kompozycji kierujących dla manipulacji sekwencjami |
| KR102271292B1 (ko) * | 2013-03-15 | 2021-07-02 | 더 제너럴 하스피탈 코포레이션 | Rna-안내 게놈 편집의 특이성을 증가시키기 위한 rna-안내 foki 뉴클레아제(rfn)의 용도 |
| US11414695B2 (en) | 2013-05-29 | 2022-08-16 | Agilent Technologies, Inc. | Nucleic acid enrichment using Cas9 |
| ES2987399T3 (es) | 2013-06-05 | 2024-11-14 | Univ Duke | Edición génica guiada por ARN y regulación génica |
| EP3825406A1 (en) * | 2013-06-17 | 2021-05-26 | The Broad Institute Inc. | Delivery and use of the crispr-cas systems, vectors and compositions for hepatic targeting and therapy |
| ES2777217T3 (es) * | 2013-06-17 | 2020-08-04 | Broad Inst Inc | Suministro, modificación y optimización de sistemas de guía en tándem, métodos y composiciones para la manipulación de secuencias |
| US10287590B2 (en) | 2014-02-12 | 2019-05-14 | Dna2.0, Inc. | Methods for generating libraries with co-varying regions of polynuleotides for genome modification |
| JP6336140B2 (ja) * | 2014-06-23 | 2018-06-06 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． | ヌクレアーゼ媒介ｄｎａアセンブリ |
| WO2016109255A1 (en) * | 2014-12-30 | 2016-07-07 | University Of South Florida | Methods and compositions for cloning into large vectors |
| US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| FI3430134T3 (fi) * | 2015-06-18 | 2023-01-13 | Uusia CRISPR-entsyymejä ja järjestelmiä | |
| WO2016205759A1 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
| US20190330659A1 (en) | 2016-07-15 | 2019-10-31 | Zymergen Inc. | Scarless dna assembly and genome editing using crispr/cpf1 and dna ligase |
| EP3491130B1 (en) * | 2016-07-28 | 2022-07-27 | DSM IP Assets B.V. | An assembly system for a eukaryotic cell |
| KR20190116282A (ko) | 2017-02-10 | 2019-10-14 | 지머젠 인코포레이티드 | 복수의 숙주를 위한 다중 dna 구조체의 조립 및 편집을 위한 모듈식 범용 플라스미드 디자인 전략 |
| JP2021533773A (ja) * | 2018-08-15 | 2021-12-09 | ザイマージェン インコーポレイテッド | ハイスループット代謝操作におけるＣＲＩＳＰＲｉの適用 |
-
2018
- 2018-02-09 KR KR1020197022014A patent/KR20190116282A/ko not_active Withdrawn
- 2018-02-09 WO PCT/US2018/017573 patent/WO2018148511A1/en not_active Ceased
- 2018-02-09 CA CA3049989A patent/CA3049989A1/en active Pending
- 2018-02-09 JP JP2019539756A patent/JP2020507312A/ja not_active Withdrawn
- 2018-02-09 EP EP18751852.7A patent/EP3580337A4/en not_active Withdrawn
- 2018-02-09 CN CN201880012880.0A patent/CN110312797A/zh active Pending
-
2019
- 2019-08-08 US US16/535,245 patent/US11098305B2/en not_active Expired - Fee Related
-
2021
- 2021-08-03 US US17/392,366 patent/US20210371856A1/en not_active Abandoned
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