CN110312797A - 组装和编辑用于多个宿主的多个dna构建体的模块化通用质粒设计策略 - Google Patents
组装和编辑用于多个宿主的多个dna构建体的模块化通用质粒设计策略 Download PDFInfo
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- CN110312797A CN110312797A CN201880012880.0A CN201880012880A CN110312797A CN 110312797 A CN110312797 A CN 110312797A CN 201880012880 A CN201880012880 A CN 201880012880A CN 110312797 A CN110312797 A CN 110312797A
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3517—Marker; Tag
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762457493P | 2017-02-10 | 2017-02-10 | |
| US62/457,493 | 2017-02-10 | ||
| PCT/US2018/017573 WO2018148511A1 (en) | 2017-02-10 | 2018-02-09 | A modular universal plasmid design strategy for the assembly and editing of multiple dna constructs for multiple hosts |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110312797A true CN110312797A (zh) | 2019-10-08 |
Family
ID=63107838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201880012880.0A Pending CN110312797A (zh) | 2017-02-10 | 2018-02-09 | 组装和编辑用于多个宿主的多个dna构建体的模块化通用质粒设计策略 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US11098305B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP3580337A4 (cg-RX-API-DMAC7.html) |
| JP (1) | JP2020507312A (cg-RX-API-DMAC7.html) |
| KR (1) | KR20190116282A (cg-RX-API-DMAC7.html) |
| CN (1) | CN110312797A (cg-RX-API-DMAC7.html) |
| CA (1) | CA3049989A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2018148511A1 (cg-RX-API-DMAC7.html) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112703250A (zh) * | 2018-08-15 | 2021-04-23 | 齐默尔根公司 | CRISPRi在高通量代谢工程中的应用 |
| CN116284436A (zh) * | 2022-11-25 | 2023-06-23 | 广州美格生物科技有限公司 | 嵌合体TsCas12a蛋白及其制备方法和应用 |
| WO2025139809A1 (zh) * | 2023-12-29 | 2025-07-03 | 武汉艾迪晶生物科技有限公司 | Cas蛋白、组合蛋白、核酸分子、核糖核蛋白复合物、重组载体、转基因细胞及其应用 |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190116282A (ko) | 2017-02-10 | 2019-10-14 | 지머젠 인코포레이티드 | 복수의 숙주를 위한 다중 dna 구조체의 조립 및 편집을 위한 모듈식 범용 플라스미드 디자인 전략 |
| CN113795588B (zh) | 2019-04-04 | 2025-02-25 | 瑞泽恩制药公司 | 用于在靶向性载体中无瘢痕引入靶向修饰的方法 |
| CN114555803B (zh) * | 2019-10-14 | 2024-11-29 | 延世大学校产学协力团 | 新的前间区序列邻近基序序列以及利用其修饰细胞基因组中靶核酸的方法 |
| KR102178813B1 (ko) | 2019-10-18 | 2020-11-13 | 주식회사 다우진유전자연구소 | Dna 구조체를 적용한 간이 키트 |
| WO2021098709A1 (zh) * | 2019-11-18 | 2021-05-27 | 中国科学院遗传与发育生物学研究所 | 衍生自黄杆菌的基因编辑系统 |
| WO2021108324A1 (en) * | 2019-11-27 | 2021-06-03 | Technical University Of Denmark | Constructs, compositions and methods thereof having improved genome editing efficiency and specificity |
| US12018316B2 (en) | 2020-03-03 | 2024-06-25 | Telesis Bio Inc. | Methods for assembling nucleic acids |
| CN113373130B (zh) * | 2021-05-31 | 2023-12-22 | 复旦大学 | Cas12蛋白、含有Cas12蛋白的基因编辑系统及应用 |
| GB202117455D0 (en) * | 2021-12-02 | 2022-01-19 | Academisch Ziekenhuis Leiden | Method of editing nucleic acid |
| US20250223620A1 (en) * | 2022-04-08 | 2025-07-10 | Glaxosmithkline Intellectual Property Development Limited | Novel processes for the production of polynucleotides including oligonucleotides |
| WO2025165780A1 (en) * | 2024-02-01 | 2025-08-07 | The Board Of Trustees Of The Leland Stanford Junior University | Combinatorial gene editing and screening |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120149115A1 (en) * | 2009-06-11 | 2012-06-14 | Snu R&Db Foundation | Targeted genomic rearrangements using site-specific nucleases |
| WO2014204726A1 (en) * | 2013-06-17 | 2014-12-24 | The Broad Institute Inc. | Delivery and use of the crispr-cas systems, vectors and compositions for hepatic targeting and therapy |
| WO2015200334A1 (en) * | 2014-06-23 | 2015-12-30 | Regeneron Pharmaceuticals, Inc. | Nuclease-mediated dna assembly |
| EP3009511A2 (en) * | 2015-06-18 | 2016-04-20 | The Broad Institute, Inc. | Novel crispr enzymes and systems |
| CN106062197A (zh) * | 2013-06-17 | 2016-10-26 | 布罗德研究所有限公司 | 用于序列操纵的串联指导系统、方法和组合物的递送、工程化和优化 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE246702T1 (de) | 1993-04-12 | 2003-08-15 | Univ Northwestern | Verfahren zur darstellung von oligonukleotiden |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| WO2000026381A2 (en) | 1998-10-30 | 2000-05-11 | Cornell Research Foundation, Inc. | High fidelity thermostable ligase and uses thereof |
| GB2481425A (en) * | 2010-06-23 | 2011-12-28 | Iti Scotland Ltd | Method and device for assembling polynucleic acid sequences |
| WO2013176772A1 (en) | 2012-05-25 | 2013-11-28 | The Regents Of The University Of California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| ES2786193T3 (es) | 2012-12-12 | 2020-10-09 | Broad Inst Inc | Modificación por tecnología genética y optimización de sistemas, métodos y composiciones enzimáticas mejorados para la manipulación de secuencias |
| EP2840140B2 (en) | 2012-12-12 | 2023-02-22 | The Broad Institute, Inc. | Crispr-Cas based method for mutation of prokaryotic cells |
| US20140310830A1 (en) | 2012-12-12 | 2014-10-16 | Feng Zhang | CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes |
| WO2014093655A2 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| PL2771468T3 (pl) | 2012-12-12 | 2015-07-31 | Broad Inst Inc | Inżynieria systemów, metod i zoptymalizowanych kompozycji kierujących dla manipulacji sekwencjami |
| KR102271292B1 (ko) * | 2013-03-15 | 2021-07-02 | 더 제너럴 하스피탈 코포레이션 | Rna-안내 게놈 편집의 특이성을 증가시키기 위한 rna-안내 foki 뉴클레아제(rfn)의 용도 |
| US11414695B2 (en) | 2013-05-29 | 2022-08-16 | Agilent Technologies, Inc. | Nucleic acid enrichment using Cas9 |
| ES2987399T3 (es) | 2013-06-05 | 2024-11-14 | Univ Duke | Edición génica guiada por ARN y regulación génica |
| US10287590B2 (en) | 2014-02-12 | 2019-05-14 | Dna2.0, Inc. | Methods for generating libraries with co-varying regions of polynuleotides for genome modification |
| WO2016109255A1 (en) * | 2014-12-30 | 2016-07-07 | University Of South Florida | Methods and compositions for cloning into large vectors |
| FI3430134T3 (fi) * | 2015-06-18 | 2023-01-13 | Uusia CRISPR-entsyymejä ja järjestelmiä | |
| WO2016205759A1 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
| US20190330659A1 (en) | 2016-07-15 | 2019-10-31 | Zymergen Inc. | Scarless dna assembly and genome editing using crispr/cpf1 and dna ligase |
| EP3491130B1 (en) * | 2016-07-28 | 2022-07-27 | DSM IP Assets B.V. | An assembly system for a eukaryotic cell |
| KR20190116282A (ko) | 2017-02-10 | 2019-10-14 | 지머젠 인코포레이티드 | 복수의 숙주를 위한 다중 dna 구조체의 조립 및 편집을 위한 모듈식 범용 플라스미드 디자인 전략 |
| JP2021533773A (ja) * | 2018-08-15 | 2021-12-09 | ザイマージェン インコーポレイテッド | ハイスループット代謝操作におけるCRISPRiの適用 |
-
2018
- 2018-02-09 KR KR1020197022014A patent/KR20190116282A/ko not_active Withdrawn
- 2018-02-09 WO PCT/US2018/017573 patent/WO2018148511A1/en not_active Ceased
- 2018-02-09 CA CA3049989A patent/CA3049989A1/en active Pending
- 2018-02-09 JP JP2019539756A patent/JP2020507312A/ja not_active Withdrawn
- 2018-02-09 EP EP18751852.7A patent/EP3580337A4/en not_active Withdrawn
- 2018-02-09 CN CN201880012880.0A patent/CN110312797A/zh active Pending
-
2019
- 2019-08-08 US US16/535,245 patent/US11098305B2/en not_active Expired - Fee Related
-
2021
- 2021-08-03 US US17/392,366 patent/US20210371856A1/en not_active Abandoned
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120149115A1 (en) * | 2009-06-11 | 2012-06-14 | Snu R&Db Foundation | Targeted genomic rearrangements using site-specific nucleases |
| WO2014204726A1 (en) * | 2013-06-17 | 2014-12-24 | The Broad Institute Inc. | Delivery and use of the crispr-cas systems, vectors and compositions for hepatic targeting and therapy |
| CN106062197A (zh) * | 2013-06-17 | 2016-10-26 | 布罗德研究所有限公司 | 用于序列操纵的串联指导系统、方法和组合物的递送、工程化和优化 |
| WO2015200334A1 (en) * | 2014-06-23 | 2015-12-30 | Regeneron Pharmaceuticals, Inc. | Nuclease-mediated dna assembly |
| EP3009511A2 (en) * | 2015-06-18 | 2016-04-20 | The Broad Institute, Inc. | Novel crispr enzymes and systems |
Non-Patent Citations (5)
| Title |
|---|
| ANDREW A. HORWITZ 等: "Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas", 《CELL SYSTEMS》 * |
| ARTURO CASINI 等: "Bricks and blueprints: methods and standards for DNA assembly", 《NATURE REVIEWS MOLECULAR CELL BIOLOGY》 * |
| ESZTER TÓTH 等: "Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells", 《BIOLOGY DIRECT》 * |
| MATTHEW L. SCHWARTZ 等: "SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans", 《GENETICS》 * |
| TETSUSHI SAKUMA 等: "Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system", 《SCIENTIFIC REPORTS》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112703250A (zh) * | 2018-08-15 | 2021-04-23 | 齐默尔根公司 | CRISPRi在高通量代谢工程中的应用 |
| CN116284436A (zh) * | 2022-11-25 | 2023-06-23 | 广州美格生物科技有限公司 | 嵌合体TsCas12a蛋白及其制备方法和应用 |
| WO2025139809A1 (zh) * | 2023-12-29 | 2025-07-03 | 武汉艾迪晶生物科技有限公司 | Cas蛋白、组合蛋白、核酸分子、核糖核蛋白复合物、重组载体、转基因细胞及其应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| US20200123540A1 (en) | 2020-04-23 |
| KR20190116282A (ko) | 2019-10-14 |
| US11098305B2 (en) | 2021-08-24 |
| WO2018148511A1 (en) | 2018-08-16 |
| EP3580337A4 (en) | 2020-12-02 |
| JP2020507312A (ja) | 2020-03-12 |
| EP3580337A1 (en) | 2019-12-18 |
| US20210371856A1 (en) | 2021-12-02 |
| CA3049989A1 (en) | 2018-08-16 |
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